LOCUS       DASVLL010000001         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_322406,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000001 DASVLL010000000
VERSION     DASVLL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322406"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF402_00005"
     CDS             complement(<1..204)
                     /locus_tag="VF402_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX7798697.1"
                     /translation="MTAEDVERAVEALLFAAAGPLSASELAYRLPEGADVGKALAALR
                     ARYAGRGVSLEVVNDRWQFRTAPD"
     gene            complement(194..1042)
                     /locus_tag="VF402_00010"
     CDS             complement(194..1042)
                     /locus_tag="VF402_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX7798698.1"
                     /translation="MAGLEHDVLQRRLDFDAPGPEIPDDIAADETLVLDLDGYEGPLH
                     VLLALARQQKVDLLRISITRLAEQYLVFIQQARRLRFALAADYLVMASWLAYLKSRLL
                     LPKTERKTGDEPPPEELAQALAYRLKKLEAIRKAVEALTSRPQLKRDVFARGDPEATL
                     VLPSARIEANVFDLMSAYVAQRRREIERSYDPTRRIEAYSLEEARDHLREELPRLNNW
                     TALEEVAPRPVRADGPRRASYLASTLSASLELCKEGQLQLQQLAIFETLYMRKRQPVA
                     GAHNDG"
     gene            complement(1057..>1528)
                     /locus_tag="VF402_00015"
     CDS             complement(1057..>1528)
                     /locus_tag="VF402_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7798699.1"
                     /translation="FHPFRVNADMPLAMTAHVLYTAIDAKNPATTSRACIRLIRDGIG
                     YDGLILCDDLSMNALTGTLGKRARGSLKAGCDVLLHCNGHLGQMREVAEEAPGLKGAA
                     KRRTEAAMRRLLREPEPLDLAAARERFDAALRRVGASEARLDPTEGHHLAPDPA"
BASE COUNT          230 a          551 c          513 g          234 t
ORIGIN      
        1 gtccggcgcg gtgcgaaact gccagcggtc gttgacgacc tcaaggctca cgccgcgccc
       61 cgcatagcgc gcgcgcaggg cggccagcgc cttgccgaca tcggcgcctt cgggcaggcg
      121 ataggccagc tccgaggccg acaaaggccc ggcggcggcg aacagaagcg cctcgacggc
      181 gcgctcgaca tcctcagccg tcattgtgcg cccccgcgac cggctggcgt ttgcgcatgt
      241 acagcgtctc gaaaatcgcc agttgctgca actgcaactg cccctccttg cacagctcca
      301 gcgaggccga cagggtcgag gccagatagg aggcgcggcg cggcccgtcc gcgcgcaccg
      361 ggcgcggcgc gacctcttcc agcgcggtcc agttgttcag gcgcggcaat tcctcgcgca
      421 ggtgatcgcg cgcctcctcc agcgaatagg cttcgatgcg ccgcgtcgga tcgtagctgc
      481 gctcgatctc gcggcggcgc tgggcgacat aggcgctcat caggtcgaag acattggcct
      541 cgatgcgcgc cgacggcagc accagggtcg cctccgggtc gccgcgcgcg aacacgtcgc
      601 gcttcaactg cggacgcgag gtcagcgcct cgaccgcctt gcggatcgcc tccagctttt
      661 tcagccggta ggccagcgcc tgcgccagct cctccggcgg cggctcgtcg ccggtcttgc
      721 gctcggtctt cggcagcaac aggcgcgatt tcagataggc cagccacgac gccatgacca
      781 gatagtccgc cgccagcgcg aagcgcagcc gccgcgcctg ctggatgaag accagatact
      841 gctcggccag ccgggtgatc gagattctca acaggtcgac cttctgctgc cgcgccagcg
      901 ccagcagcac gtgcagcggc ccttcgtagc cgtcgaggtc cagcaccagc gtctcgtcgg
      961 cggcgatgtc gtcgggaatc tccgggccgg gcgcgtcgaa gtcgagccgc cgctgcaaca
     1021 cgtcgtgctc aagcccggcc atgtcgcgct cccggctcat gccggatcag gggccagatg
     1081 atgcccctcg gtcggatcga gacgcgcctc cgaggcgccg acgcgcctga gcgccgcgtc
     1141 gaagcgttcg cgcgccgccg cgaggtccag cggctccggc tcgcgcagca accggcgcat
     1201 cgccgcctcg gtccgtcttt tggccgcgcc tttcaggccg ggcgcctcct cggcgacctc
     1261 gcgcatctgg ccgagatggc cgttacagtg cagcaggacg tcgcaaccgg ccttcaggct
     1321 gccccgcgcg cgcttgccca gcgtgcccgt caaggcgttc atcgacaggt cgtcgcacag
     1381 gatcagtccg tcatagccga tcccgtcgcg gatcagccgg atgcaggcgc gcgaggtcgt
     1441 ggccggattt ttcgcgtcga tggcggtgta aagcacgtgc gcggtcatgg ccagaggcat
     1501 gtcggcattg accctgaacg gatggaaa
//
LOCUS       DASVLL010000002         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_107507,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000002 DASVLL010000000
VERSION     DASVLL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107507"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF402_00020"
     CDS             complement(<1..308)
                     /locus_tag="VF402_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798700.1"
                     /translation="MTSSCARAFTPPKSAPPSPPPVHRLRAEPRPSATETSLMPKTML
                     IDATHAEETRVVVMNGSRVEEFDFESHARKQLRGNIYLANVTRVEPSLQAAFVEYGGN
                     R"
     gene            894..2123
                     /locus_tag="VF402_00025"
     CDS             894..2123
                     /locus_tag="VF402_00025"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7798701.1"
                     /translation="MKRRFRTFVSLKTLFCALVMCASSVPSLIMAAPAEAAASTGGDV
                     VRVRLGGDERQTRIVVELDKSVEGRLVGAENGARQVIALPDVDLDRTMSGQGRGLIAS
                     WKIEKIAGSTRLHLDYNGKARVLRRFLLPPADGISVYRYVVDIAPQGADAAPAQAAPL
                     PVVTQTVAQAAPLPEDSALRKKVIVIDAGHGGKDPGAHGAFSLEKDINLASAKALRDA
                     LEKTGRYKVIMTRDTDSFVDLAGRVRIARNADADLFISLHSDSGGDGSTSGASIYTLS
                     DSGTERAAKKAMVRGDWSLSDTTSSDRIVNRILIDLTQRATKNRSATFAQLVMDHIGE
                     STPLLKSNLRQAGFVVLLAPDVPAVLLEMGYVNNDHDEKMLNDPRHRELMMGEVAKAI
                     DQYFANDVRYASFAGLN"
BASE COUNT          419 a          691 c          731 g          376 t
ORIGIN      
        1 cggttgccgc cgtattcgac gaaggcggct tgcaggctgg gctcgacgcg ggtgacattg
       61 gcgagataga tgttgccgcg cagttgcttg cgcgcgtggc tttcgaaatc aaattcctcg
      121 acgcgggatc cgttcatcac gacgacgcgg gtttcttccg cgtgcgtcgc gtctatcaac
      181 atggttttgg gcattaaaga cgtctccgta gcgctcggac gcggctcggc gcgcaaccgg
      241 tgaaccggcg gcggcgaagg cggggcgctt ttgggtgggg tgaaggcgcg cgcgcatgac
      301 gacgtcatgc tcaaccgagg ctccggtagg gaagatctca aaaggttgcg gggcgcggcc
      361 catgcggttg gtaatccaga ctttggttca gtgcagcgcg aaacgcgctg aaataagggg
      421 cgcgacggcg atttggggcg aaaaattctc gcggcggaca ggcgcttggc catacggtga
      481 accgcacgga tcgcgtcgcc gtgcgagatg gcgtcccggc atcgacatca caagggcgtc
      541 ggacgcggcg gcctgcaaaa attcgcgcga cagcgctgaa ccggccatgc cgcagcatcg
      601 gcgacatcct gacgcaaacc atgtcggtaa aacgcaggat gcgtcaacct atttcttcgc
      661 gtcataagcg taagggcgtc gcgcgcgaaa tttctgtgcg tgaacgcgcc gccttaaccg
      721 gattgcaacc atatttctaa agagaagtga aagcataacg cttaacgcta tttcgacctc
      781 tctcaggcat gatgacgcat acggggcgcg aagccgccgc gtttgacgga tcgacgcatc
      841 tgtccgacac gaaaaatctg gccgacatag aatatgtggt gtgtggagta acaatgaaac
      901 gacggtttcg aaccttcgtc agcctgaaga cgctgttttg cgcgctcgtc atgtgcgcgt
      961 cgtccgtccc gtccctgatc atggccgcgc ccgccgaggc ggcggcgtcc accggcggcg
     1021 acgtggtgcg cgtgcgcctg ggcggcgatg agcgccagac gcgcatcgtg gtcgaactgg
     1081 acaaaagcgt cgagggcagg ctggtcggcg ccgagaacgg cgcgcgtcag gtgatcgcgc
     1141 tgccggacgt ggacctcgac cgcacgatga gcgggcaggg gcgcggactg atcgccagtt
     1201 ggaagatcga gaagatcgcg ggcagcacgc gcctgcatct ggattataac ggcaaggcgc
     1261 gcgtcctgcg ccgtttcctt ctgccgcccg ccgacggcat cagcgtctat cgctacgtcg
     1321 tcgacatcgc gccgcagggc gccgacgcgg cgccggcgca ggccgcgccc ctgccggtcg
     1381 tcacccagac cgtcgcgcag gccgcgccct tgcctgagga cagcgcgctg cgcaagaagg
     1441 tgatcgtgat cgacgccggc cacggcggca aggatcccgg cgcgcacggc gcgttcagtc
     1501 tggaaaagga catcaacctg gcctcggcca aggccctgcg cgacgcgctg gaaaagaccg
     1561 gacgctataa ggtcatcatg acgcgcgaca ccgattcgtt cgtcgatctg gccgggcgcg
     1621 tgcgcatcgc gcgcaacgcc gacgccgacc tgttcatctc gctgcattcc gattccggcg
     1681 gcgacggctc gacctcgggc gcctcgatct acaccctgtc cgattccggc accgagcgcg
     1741 ccgcgaagaa ggcgatggtc cggggcgact ggtccttgtc ggacaccacc tcgtcggacc
     1801 gcatcgtcaa ccgcatcctg atcgacctga cccagcgcgc gaccaagaac cgctcggcca
     1861 ccttcgcgca actggtcatg gaccatatcg gcgagtcgac gccgctgctg aaaagcaatc
     1921 tgcgtcaggc cggtttcgtg gtgctgctgg cgcccgacgt gcccgcggtg ctgctggaaa
     1981 tgggctatgt caacaacgat cacgacgaaa agatgctcaa cgatccgcgc catcgcgaac
     2041 tgatgatggg cgaggtcgcc aaggcgatcg accagtattt cgccaacgac gtgcgctacg
     2101 catcgttcgc cggactgaat taagaacggc gcgcaaagac ctgatatctc gacgcttatg
     2161 gccccgcacg acctgcgggg ccatcggcgt ttccggcccg gcgcgcagag gggcggc
//
LOCUS       DASVLL010000003         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_322434,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000003 DASVLL010000000
VERSION     DASVLL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322434"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /gene="holA"
                     /locus_tag="VF402_00030"
     CDS             complement(<1..959)
                     /gene="holA"
                     /locus_tag="VF402_00030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7798702.1"
                     /translation="MKLAKRAEIDAFFAKPPSAVLGALIYGKDRSQVIERATGLAKQI
                     VPDINDPFNVSVLTDSDIDADPARLEGELQALSMMGGRRLVRLKFFSEKASLDKAVAA
                     ALKAHAAGEFNRDAFIVIEAGALGGDSALRRLADADKALVSVACYEDETGDVVRMARE
                     TLSANGVALTTAAMDIFAQRLPKERGIARQEIERLILFIGPGSHKTLNETELLDFLGV
                     EPEASLFKAAQDAFGGRMKPAQAGLRRAFAEGEGGADALRALSLHYNKLKLLKSLLEK
                     GMGTKEAARAAGVFWKDENDLLRQAQAWRFDALDALAADLIEA"
     gene            complement(956..1468)
                     /gene="lptE"
                     /locus_tag="VF402_00035"
     CDS             complement(956..1468)
                     /gene="lptE"
                     /locus_tag="VF402_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX7798703.1"
                     /translation="MRQTVRALGAAACLAVLPLLSACGFVPLYAQTGVTSGLSAIEVD
                     APQTRTGYFLEQDLRNGLATDATSDKRYTLKIAMKEQHFAVGYQVDDTSLRSEITAQV
                     SYNLIDKTTGKSLYKDAFSDTVTYDTTRSPFTGVVAQQDAQKRIATDISGKIQTALAL
                     YFHGDAPKAP"
     gene            complement(1475..>1690)
                     /locus_tag="VF402_00040"
     CDS             complement(1475..>1690)
                     /locus_tag="VF402_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798704.1"
                     /translation="FDPALAQDDAYVMPVQVNGKRRGELLIRVGASEDEIRDAALADE
                     AVKRHLEGVSIRKIVFVPNRILNFVVG"
BASE COUNT          264 a          551 c          562 g          313 t
ORIGIN      
        1 tcggcttcga tcaggtcggc ggcgagggcg tcgagcgcgt cgaaacgcca ggcctgcgcc
       61 tgacgcaaca ggtcgttttc gtccttccag aagaccccgg cggcgcgcgc ggcctccttg
      121 gttcccatgc ctttttccag cagggatttg agcagtttca gcttgttgta atgcaggctc
      181 agcgcgcgca gggcgtcggc gccgccttcg ccctcggcga aagcgcggcg caaaccggcc
      241 tgcgccggtt tcatgcggcc gccgaacgcg tcctgcgccg ccttgaacag ggacgcttcc
      301 ggctcgacgc cgagaaaatc cagaagttcg gtttcgttca gcgtcttatg cgaaccgggg
      361 ccgatgaaca ggatcagccg ctcgatctcc tgccgcgcga tgccgcgctc tttcggcagg
      421 cgctgggcga agatgtccat cgccgccgtg gtcagggcca cgccgttggc gctcagcgtc
      481 tcgcgcgcca tgcgcaccac gtcgccggtc tcgtcctcgt agcaggcgac gctgaccagc
      541 gccttgtccg cgtcggccag ccgccgcagc gccgaatcgc cgcccagcgc gcccgcttcg
      601 atgacgataa aggcgtcgcg gttgaattcg cccgccgcat gggctttcag cgccgcggcg
      661 accgccttgt cgagcgacgc cttctccgaa aagaatttca accgcaccag ccgccgcccg
      721 cccatcatcg acagggcctg caactcgcct tccagccgcg ccggatcggc gtcgatgtcg
      781 ctgtcggtca gcaccgagac gttgaacgga tcgttgatgt cgggaacgat ctgcttcgcc
      841 agccccgtgg cgcgctcgat cacctggctg cggtccttgc cgtagatcag cgcgccgagg
      901 accgcggacg ggggtttggc gaaaaaggcg tcgatttcgg cccgtttggc gagcttcatg
      961 gcgctttggg cgcgtcgccg tggaaataga gggcgagcgc ggtctggatc ttgccggaaa
     1021 tgtcggtggc gatgcgcttt tgcgcgtcct gctgcgccac cacgccggta aagggcgagc
     1081 gcgtggtgtc gtaggtgacg gtgtcgctga acgcgtcctt atagaggctc ttgccggtcg
     1141 tcttgtcgat caggttgtac gacacctgcg cggtgatttc cgaacgcagc gaggtgtcgt
     1201 cgacctgata gccgacggcg aagtgctgct ccttcatggc gatcttgagc gtatagcgct
     1261 tgtcgctggt ggcgtcggtg gccagaccgt tgcgcaaatc ctgttccaga aaatagccgg
     1321 tgcgggtctg cggcgcgtcg acctcgatgg ccgacaggcc gctggtcacg cccgtttgcg
     1381 cgtagagcgg cacgaagccg cacgccgaca gcagcggaag cacggccaga caggcggcgg
     1441 cgccgagggc tcgaacggtc tggcgcatca ggttttatcc tacgacgaaa ttgagaatcc
     1501 ggttcggcac gaagacgatc ttgcggatcg agacgccttc aagatgacgc ttaaccgcct
     1561 cgtccgcaag cgcggcgtcg cggatttcgt cctcggaggc gccgacgcgg atcagcagtt
     1621 cgccgcgccg cttgccgttg acctgcaccg gcatgacgta ggcgtcgtcc tgcgccagcg
     1681 ccggatcgaa
//
LOCUS       DASVLL010000004         5730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_376310,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000004 DASVLL010000000
VERSION     DASVLL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376310"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VF402_00045"
     CDS             complement(<1..175)
                     /locus_tag="VF402_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7798705.1"
                     /translation="MIDFPHLQFGHGEAIDALREAVRRFAAAEIAPLAEAADREDRFP
                     MPLWRKLGDIGALG"
     gene            complement(185..1891)
                     /locus_tag="VF402_00050"
     CDS             complement(185..1891)
                     /locus_tag="VF402_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7798706.1"
                     /translation="MFTRILIANRGEIARRIIRTCRRMQVETVAVYSEADKHAPHVAE
                     ADRAVLIGPPPAADSYLRGDRIVAVALETGAQAIHPGYGFLSENAGFAQACEAAGLAF
                     IGPPPDAIRAMALKGAAKARMAAAGVPVTPGYHGDDQNPEHLLREAARIGYPVLIKAV
                     AGGGGKGMRRVDAPEDFPDALASAQREAQSAFADAKVLIETCVATPRHVEIQVFADSH
                     GHVVHLFERDCSMQRRHQKVIEEAPAPGLPDALRQAMGAAAVRAAKAIGYRGAGTVEF
                     IVDVSKGAAAANFWFMEMNTRLQVEHPVTEAITGLDLVEWQLRVAAGEPLPKTQGQIA
                     LNGHAVEARLYAEDPHNDFLPATGTLTRLDFPPVRVESAVAAGDAVSVFYDPMIAKLI
                     AHGRDRAAAIAALREALIRTRVEGVTTNLGFLRRLVSDADFAAAKLDTGLIARRLDAL
                     LTPWPDAAPCTDDSQKSDPWTDPTGWRVNLPPADPLWLGTDAAGERVVTGEVFAPMPG
                     AVLDVFVAAGQAVTKGDKLLILGAMKIEHTLKAPKDGVARAVHAAAGDQVADKALLVE
                     IE"
     gene            complement(1891..2661)
                     /locus_tag="VF402_00055"
     CDS             complement(1891..2661)
                     /locus_tag="VF402_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7798707.1"
                     /translation="MKTLMLDSDARGVVRLTLNRPQVHNAFDETMIAELHETFDALGQ
                     QAGVRAIVLTGAGASFSAGADVTWMRRAADMDEAANRADALRMADMFACMNACPRPVI
                     GLVNGAAFGGGVGLVACCDIVIAGPGAVFGLTEVRLGLIPAVIAPFVIARTGAGAARR
                     YMLTGERFDAATARRIGLVHEIMAAPDAAPVLDALLQGAPGAVAEAKALIHDVSRHAP
                     GDDLRHLTARRIAARRVSDEGRAGVDAFLARRKPDWGM"
     gene            complement(2671..4278)
                     /locus_tag="VF402_00060"
     CDS             complement(2671..4278)
                     /locus_tag="VF402_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7798708.1"
                     /translation="MSVLKSQIDTHDDGFLANVAHMSARVRDLRDLTARIAQGGSAAA
                     RERHVARGKLLPRERVTGLLDPGAPFLEFSALAAHGVYDEDAPAAGIITGIGRIGGTE
                     CVVVCNDATVKGGAYFPLTVKKHLRAQEIAMQNNLSCVYLVDSGGANLPHQDEVFPDK
                     DDFGRIFFNQARMSAQGVAQIAVVMGSCTAGGAYVPAMSDESVIVQKQGTIFLAGPPL
                     VKAATGETVSAEDLGGGDVHARVSGVADHLADDDRDALRIARRIVGHLNHRKPAGPVT
                     RPAAEPLYAAEEMYGVVPADARQPFDAREIIARLVDGSEFDEFKALYGETLVCGFAHL
                     YGFPVGIVANNGVLFSESALKGAHFVELCAQRGVPLIFLQNVTGFMVGRRYENEGIAR
                     HGAKMVAAVACADVPKLTVIVGGSYGAGNYGMCGRAFEPRFLWMWPNARISVMGGEQA
                     ANVLATVRRDSIEAGGGHWSAADEAAFKAPIRATYEAQGHPYYASARLWDDGVIDPAD
                     TRRVLGLGLSAALNAPIPPTRFGVFRM"
     gene            4353..5231
                     /locus_tag="VF402_00065"
     CDS             4353..5231
                     /locus_tag="VF402_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7798709.1"
                     /translation="MELYLLRYFLAVVETGGFTRAAAACLITQPTLSAGIKRLEDELG
                     TALFVRSNRRVHLTAAGARFLPRAKAILHEVNMASAEIAGTEAANVLRLGVLQTVPAR
                     LVADLLRGFADAHPGVRFDLFDGDEQALLNRLDERGLDMALSIRRVADDDAAPLLTEG
                     YRLVLPRGHRLAGQAVIAAEDLARDAMIVRSRCEVLSETSRHFTDRNVRPPLAYRTAQ
                     DERALVMVGAGVGVTVMPDSYDAPDVARAVLHGFTPTRTLALFPPRYALSGRLLDAAR
                     AFTQFARLFFKVDDLG"
     gene            complement(5163..>5730)
                     /locus_tag="VF402_00070"
     CDS             complement(5163..>5730)
                     /locus_tag="VF402_00070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7798710.1"
                     /translation="VEGQFDRIVSVGMFEHVGKAAFQTYFDTVYDRLKDDGVALIHSI
                     GRRGPSGARSDWLDKYIFPGGYIPSLSEIMTSVEKSGLWVTDVEPLRLHYAKTLRCWR
                     ERFMDHRLEIEDLYDDRFCRMWEFYLCWCELGFRYSDLMVFQVQVAKRVDTLPITRDY
                     MMAREMSAEVVNFEEEARKLRERPRRVQ"
BASE COUNT          870 a         2034 c         1954 g          872 t
ORIGIN      
        1 gacccagcgc gccgatatcg cccagcttgc gccacagagg catcgggaag cggtcctcgc
       61 ggtcggcggc ctcggccagg ggcgcgatct cggcggcggc gaagcggcgc acggcctcgc
      121 gcaaggcgtc gatcgcctcg ccgtggccga attgcagatg cggaaagtcg atcatggcgg
      181 gcctctattc gatctcgacc agcagcgcct tgtcggcgac ctgatccccg gcggcggcgt
      241 ggacggcgcg cgccacgccg tccttcggcg ccttcagcgt atgttcgatc ttcatggcgc
      301 cgaggatcag cagcttgtcg cccttcgtca ccgcctgccc cgccgcgacg aaaacgtcca
      361 gcacggcgcc gggcatgggc gcgaacacct cgcccgtcac gacgcgctcg cccgccgcgt
      421 ccgtccccag ccacaacgga tcggcggggg gcagattgac gcgccagccg gtcgggtccg
      481 tccacgggtc gcttttttga gaatcgtccg tacacggcgc cgcgtccggc cacggcgtca
      541 gcagcgcgtc gaggcgccgg gcgatcaagc ccgtgtccag cttcgcggcg gcgaaatcgg
      601 cgtcggaaac cagacggcgc agaaagccga gattggtcgt cacaccttcg acgcgcgtgc
      661 ggatcagcgc ctcgcgcaag gccgcgatcg ccgccgcgcg gtcgcgcccg tgcgcgatca
      721 gcttggcgat catcgggtcg tagaacacgc tgaccgcatc gcccgccgcg accgcgctct
      781 cgacgcgcac gggcggaaag tcgagccgcg tcagcgtccc ggtcgcgggc agaaaatcgt
      841 tgtgcgggtc ttcggcgtaa agccgcgcct cgacggcatg gccgttcagc gcgatctgcc
      901 cttgcgtctt cggcagcggc tcgcccgccg cgacgcgcaa ctgccattcg accagatcga
      961 ggccggtgat cgcctccgtc accggatgtt cgacctgaag ccgtgtgttc atctccatga
     1021 accagaagtt cgccgccgcg gcgcctttgg aaacatcgac gatgaactcc acggtcccgg
     1081 cgccgcgata gccgatggcc ttggccgcgc gcaccgccgc cgcgcccatc gcctgacgca
     1141 aggcgtcggg caggccgggc gcgggcgcct cctcgatcac cttctggtgg cggcgctgca
     1201 tcgaacagtc gcgctcgaac agatgcacga catggccgtg gctgtcggcg aacacctgaa
     1261 tctcgacatg gcgcggcgtg gcgacgcagg tttcgatcag caccttcgca tcggcgaagg
     1321 ccgattgcgc ctcgcgctgc gccgaggcca gcgcgtcggg gaaatcctca ggcgcgtcga
     1381 cgcggcgcat ccccttgccg ccgccgcccg ccaccgcctt gatcagcacc ggatagccga
     1441 tgcgcgcggc ctcgcgcaac agatgctccg ggttctgatc gtcgccgtga tagccgggcg
     1501 tgaccggcac gcccgccgcc gccatccgcg ccttggccgc gcctttcagc gccatcgcgc
     1561 ggatcgcgtc gggcggcggg ccgatgaagg cgaggcccgc cgcttcgcac gcctgcgcga
     1621 acccggcgtt ttccgacagg aagccatagc cgggatggat cgcctgcgcg cccgtctcca
     1681 gcgccacggc gacgatgcgg tcgccgcgca ggtacgagtc ggcggcgggc ggcgggccga
     1741 tcagcacggc ccggtcggcc tccgcgacgt gcggcgcgtg tttgtcggcc tcggaataga
     1801 cggccaccgt ctccacctgc atgcggcggc aggtgcggat gatgcggcgg gcgatctcgc
     1861 cccggttggc gatcaggatg cgcgtgaaca tcacatcccc cagtccggtt tgcggcgggc
     1921 gagaaaggcg tcgacgcccg cgcggccttc gtccgacacg cgccgggcgg cgatgcgccg
     1981 cgcggtcaga tggcgcaggt cgtcgccggg cgcatggcgc gaaacgtcat gaatcagcgc
     2041 cttggcctcg gccaccgcgc cgggcgcgcc ttgcagcagg gcgtcgagga ccggcgcggc
     2101 gtcgggcgcc gccatgatct catgcacgag gccgatgcgc cgggccgtcg cggcgtcgaa
     2161 acgctcgccg gtcagcatgt agcggcgcgc ggcgccggcg ccggttctgg cgatcacgaa
     2221 cggcgcgatc accgccggga tcaggcccag ccgcacttcg gtcaggccga acacggcgcc
     2281 gggtccggcg atcacgatgt cgcagcacgc gaccagcccg accccgccgc cgaaggccgc
     2341 gccgttgacg aggccgatca ccggccgggg gcaggcgttc atgcaggcga acatgtcggc
     2401 catgcgcagc gcgtccgcgc ggttggccgc ttcgtccatg tccgccgcgc gccgcatcca
     2461 cgtcacgtcg gccccggcgg agaaactcgc gcccgccccg gtcagcacga tggcgcgcac
     2521 gcctgcctgc tggcccagcg cgtcgaacgt ctcatgcagt tcggcgatca tggtctcgtc
     2581 gaaggcgttg tgaacctgcg ggcggttcag ggtcaggcgg accacgccgc gcgcatcgct
     2641 gtccagcatc agggttttca tctttggacc tcacatgcgg aaaacgccga accgggtcgg
     2701 cgggatcggg gcgttgagcg ccgcgctcag gccgaggccc agcacgcggc gggtgtcggc
     2761 gggatcgatc acgccgtcgt cccacaaacg cgccgaggcg taatagggat ggccctgcgc
     2821 ctcgtaggtc gcgcggatcg gcgccttgaa ggccgcctcg tccgcggcgc tccaatgccc
     2881 gcccccggct tcgatagagt cgcgcctgac cgtggccagc acattggccg cctgctcgcc
     2941 gcccatgacc gagatgcgcg cgttcggcca catccataaa aagcgcggct cgaaggcgcg
     3001 gccgcacatg ccgtaattgc ccgcgccata ggagccgccg acgatgacgg tcagcttggg
     3061 cacatcggcg caggcgaccg ccgcgaccat cttggcgccg tggcgggcta tgccttcgtt
     3121 ctcgtatctg cgcccgacca tgaagccggt gacgttctgc aagaagatca gcggcacgcc
     3181 gcgctgggcg cacagctcga cgaagtgcgc gcctttcagc gcgctttcgg agaacagcac
     3241 gccgttattg gcgacgatcc cgaccggaaa gccgtaaaga tgcgcgaagc cgcagacgag
     3301 cgtttcgccg tacagcgcct tgaactcgtc gaactcagag ccgtcgacca gccgcgcgat
     3361 gatctcgcgc gcgtcgaagg gctggcgcgc gtcggcgggg acgacgccgt acatctcctc
     3421 cgccgcatag agcggctcgg cggcggggcg cgtgaccggc ccggccggtt tcctgtggtt
     3481 gagatggccg acgatgcggc gcgcgatgcg cagggcgtcg cggtcgtcgt cggccagatg
     3541 gtcggccacg ccggagacgc gcgcatgcac gtcgccgccg cccagatcct cggcgctgac
     3601 cgtctcgccg gtcgcggcct tgaccagagg cggtccggcc agaaagatcg tgccttgctt
     3661 ctgcacgatc acgctttcgt cgctcatcgc cgggacatag gcgccgcccg ccgtgcagga
     3721 gcccatgacg acggcgatct gcgcgacgcc ttgcgccgac atgcgcgcct gattgaagaa
     3781 gatgcggccg aaatcgtcct tgtccgggaa cacctcgtcc tgatgcggca ggttggcgcc
     3841 gccggaatcg accagataga cgcacgacag gttgttctgc atcgcgatct cctgcgcgcg
     3901 caggtgcttc ttgaccgtga gcggaaaata ggcgccgccc ttgaccgtcg cgtcgttgca
     3961 cacgaccacg cattcggtcc cgccgatgcg gccgatgccg gtgatgatcc cggcggcggg
     4021 cgcgtcctcg tcatagaccc cgtgcgccgc cagggccgag aactccagaa acggcgcgcc
     4081 gggatcgagc aggccggtga cgcgctcgcg cggcaacagc ttgccgcgcg ccacatgccg
     4141 ttcgcgcgcg gcggccgaac cgccctgcgc gatgcgggcg gtcaggtcgc gcaagtcccg
     4201 gacccgcgcg ctcatatgcg cgacgttggc gaggaacccg tcgtcatgcg tgtcgatctg
     4261 gcttttcagg acgctcatgg cgacagctta actccgccgt atggattgga aaaatcgata
     4321 gaaaaaattc tcatcataga tatgatctat gtatggagct ttacctgctg cgctattttc
     4381 tggccgtggt cgagacgggc ggcttcaccc gcgcggcggc ggcctgcctg atcacccagc
     4441 cgaccctgtc cgccggcatc aagcggctgg aggacgaact ggggaccgcc ctgttcgtgc
     4501 gctcgaaccg gcgcgtacac ctgacggcgg cgggggcgcg cttcctgccg cgcgccaagg
     4561 cgatcctgca cgaggtcaat atggccagcg ccgagatcgc cgggaccgag gcggccaacg
     4621 tgctgcggct cggcgtgttg cagaccgtcc cggcgcgcct cgtcgccgac ctgctgcgcg
     4681 gcttcgccga tgcgcatccc ggcgtgcgct tcgacctgtt cgacggcgac gagcaggccc
     4741 tgctcaaccg gctggacgaa cgcgggctgg acatggcgct gtcgatccgg cgcgtggcgg
     4801 acgacgacgc ggcgccgctg ttgaccgaag gctaccggct ggtcctgccg cgcgggcacc
     4861 ggctggccgg acaggcggtg atcgcggccg aggatctggc gcgcgacgcc atgatcgtgc
     4921 gttcgcgctg cgaggttctg tccgaaacca gccgccactt caccgaccgc aatgtgcgcc
     4981 cgccgctggc ctaccgcacc gcgcaggacg agcgcgccct cgtcatggtc ggcgcgggcg
     5041 tcggcgtcac cgtcatgccg gacagctatg acgcgcccga cgtggcgcgc gccgttctgc
     5101 acggcttcac gccgacgcgc accctggccc tgttcccgcc gcgctacgcc ttgtcggggc
     5161 ggctactgga cgcggcgcgg gcgttcacgc agtttgcgcg cctcttcttc aaagttgacg
     5221 acctcggctg acatttcgcg cgccatcatg tagtcgcgcg tgatcggcag cgtgtcgacg
     5281 cgcttggcca cctgcacctg gaacaccatc aggtcggaat agcggaagcc cagctcgcac
     5341 cagcacagat agaactccca catgcggcag aagcggtcgt cgtacagatc ctcgatctcc
     5401 agccggtgat ccatgaagcg ctcgcgccag cagcgcagcg tcttggcgta gtgcagccgc
     5461 agcggctcga cgtccgtgac ccacaggccg gatttttcca ccgaggtcat gatctcggac
     5521 aggctgggga tatagccgcc ggggaagatg tacttgtcca gccagtccga gcgcgcgccc
     5581 gacggtccgc gccgcccgat cgagtggatc agcgccacgc cgtcgtcttt cagccggtcg
     5641 taaaccgtgt cgaaataggt ctggaacgcc gccttgccga catgctcgaa catgccgacg
     5701 ctgacgatgc ggtcgaactg gccctcgacc
//
LOCUS       DASVLL010000005         5637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_53997, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000005 DASVLL010000000
VERSION     DASVLL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53997"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF402_00075"
     CDS             complement(<1..221)
                     /locus_tag="VF402_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798711.1"
                     /translation="MSGLFDAPGPRWFNIPAGRDVLRDLARGLSEALRDRLSEAQVLT
                     PTRRGARAMARAFSGLASVRDDKALLLPQ"
     gene            complement(218..931)
                     /locus_tag="VF402_00080"
     CDS             complement(218..931)
                     /locus_tag="VF402_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7798712.1"
                     /translation="MSGAPETAFILAAGLGTRMRPLTDDRPKSLVEVAGKTLLDHMLD
                     RLKAAGAKRFVVNVHYFADRLEAHLKTRADLDIVISDERAALLETGGGLKKARDLLGG
                     DAIWVANTDSVWSGDDEALDRLAAAWRPEAMDACLLLAPMARTTGFDGAGDFFLAPDG
                     RLSFRNDAPAAPFNYMGVHITKPAIVDDEPEAAFSLAKIWRRVAPRGRLHGVVMQGDW
                     MHVGDPAARDAADAHLRGR"
     gene            complement(928..2031)
                     /locus_tag="VF402_00085"
     CDS             complement(928..2031)
                     /locus_tag="VF402_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7798713.1"
                     /translation="MTDIIDREAIKQGFLLSAGFAGAHRQPLPGDASTRRYERLTQAN
                     GATFMLMDQPPALESQPCRLHETQDERRASGYNAMARLAAGRIEAFVAASSYLRAQGL
                     SAPEIYEVDAANGLLISEDLGDDLFARLIERGQDETPLYLSAVEAQARLHALTPPDVL
                     SPEGEYGWPLLTYDDLALKTGADLFIEWFPKFDSGARIGDEAAAEWEALWAPLRRRAE
                     AGATAFIHRDYHAENLLWLPQRDGAARVGMIDFQDCLRAHPSWDLLSLLQDARRDVSP
                     ELEAAALDHYFALRPQIDRDAFMADYAALATLNEARILGIFARLVTRDAKPRYAAFMP
                     RMWRHLGRNLRQPGMEGLLGWFKRHGFAERIAA"
     gene            complement(2028..2516)
                     /gene="tsaE"
                     /locus_tag="VF402_00090"
     CDS             complement(2028..2516)
                     /gene="tsaE"
                     /locus_tag="VF402_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7798714.1"
                     /translation="MAEPLDLSRPQRFVLPDEAATARLGAKIAPLLRAGDVVYLRGGL
                     GMGKSSLARGLIRALTAPDQDVPSPTFTLMQIYAAADFDVLHLDLYRLTDPEEAYELG
                     LDELAPASVLLIEWPDRLGHLGFDDRLEIGLEMADKTNSPDAGQGRIAALTPIGRFRD
                     DL"
     gene            complement(2509..3324)
                     /locus_tag="VF402_00095"
     CDS             complement(2509..3324)
                     /locus_tag="VF402_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX7798715.1"
                     /translation="MHIIDELIYERAPRLVKSPFWPFVRPPLYALLGYPKAVGMADAI
                     RDLSGEDALDYVSRLLALEVAARRLDRLPQTGRCLVVCNHPTGIADGVAVRDALKRRR
                     DDIVFFANADALRVNPRFDESLIPVEWVEAKRTRDKTRATLQAAKSAFEREACVVMFP
                     AGRLARRDAAGALIDPPWAGTCASLAQKYGAPIVPAYLSGPDSVWFHLFNRFSRELRD
                     ITLFHEFLNKAGKRFDLSLGPPIDPDTDDLDAERLKAYVETTLRQQPDAPYHG"
     gene            3390..3533
                     /locus_tag="VF402_00100"
     CDS             3390..3533
                     /locus_tag="VF402_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798716.1"
                     /translation="MTTPAPTSKPRPESEDRRHPKAFFPYLILWAVCLACLAFAAVML
                     IAA"
     gene            3661..4422
                     /locus_tag="VF402_00105"
     CDS             3661..4422
                     /locus_tag="VF402_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798717.1"
                     /translation="MSRKALLWAMALVVIVPALIGGGYWIWWDQVEKYAPVAIANPDD
                     ASAIQSLLDRVDYVSPGPRGRWVYLIGYRNCAPCNAYQRSEFARLQAAGVETRVVMFA
                     RPDNGAQKQSTPQERSTVAELWLNRSWPLYLQWFNAPEGDWKAVGLPVADDDMARSGV
                     VWASREFIAELTPVLARNRVHAGYPLVIWRDANRQLRVCACDSDKAYHFIREDFGVKG
                     DLEQAAQNIIDLPGKLIGGTSSAASADYGPDGQAQ"
     gene            complement(4809..5252)
                     /gene="rplS"
                     /locus_tag="VF402_00110"
     CDS             complement(4809..5252)
                     /gene="rplS"
                     /locus_tag="VF402_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX7798718.1"
                     /translation="MNLLQQIEAEEVAKLKGDKTFPQFQPGDTLRINVKIKEGDRERV
                     QAFEGVCIARAGSGINETFTVRKISFGEGVERKFPILSPNIESIEVKRRGVVRRAKLY
                     YLRDRRGKSARIAERSMNSTRGSKGAELRAQMAAAEASGNPDAEA"
     gene            complement(5273..>5637)
                     /locus_tag="VF402_00115"
     CDS             complement(5273..>5637)
                     /locus_tag="VF402_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7798719.1"
                     /translation="RVLDARGFEEISVGDAVLAGGEAAAMVAIEAVVRLLPGVLGAAA
                     SLERESFEDGLLEEPQFTRPRSFEGLDIPDVLLSGDHKKMAQWKQQQREATTKERRPD
                     LWEAYLSNLQANGASKRK"
BASE COUNT          904 a         1871 c         1909 g          953 t
ORIGIN      
        1 atttgcggca gcagcagcgc cttgtcgtcc ctgaccgacg ccagcccgga aaaggcccgc
       61 gccatcgcgc gcgcgccgcg ccgcgtcggc gtcagcacct gcgcctccga cagccggtcg
      121 cgcaaagcct cgctcaggcc gcgcgcgaga tcgcgcaaca cgtcgcggcc cgccggaatg
      181 ttgaaccagc gcggaccggg cgcgtcgaac aggccgctca tcgccccctc agatgcgcgt
      241 ccgccgcatc gcgcgcggcg ggatcgccga cgtgcatcca gtcgccctgc atgacgacgc
      301 catgcaggcg gccccgcggc gcgacccggc gccaaatctt ggccagcgag aaggcggcct
      361 ccggctcatc gtcgacgatg gcgggcttgg ttatatgcac gcccatatag ttgaacggcg
      421 cggcgggcgc gtcgttgcga aagctcaggc gcccgtcggg ggccaggaaa aagtcgcccg
      481 cgccgtcgaa gccggtggtg cgcgccatcg gcgccagcag caggcaggcg tccatcgcct
      541 ccggccgcca cgcggcggcg agccggtcca gcgcttcgtc gtcgccgctc cagacgctgt
      601 cggtattggc gacccagatg gcgtcgccgc cgagcaggtc gcgcgccttt ttcagcccgc
      661 cgccggtctc cagcagcgcc gcgcgctcgt cggaaatcac gatgtcgagg tcggcgcgcg
      721 tctttaaatg cgcctccagc ctgtcggcga aatagtggac attgaccacg aaacgcttgg
      781 cgcccgccgc cttcagccgg tccagcatgt gatcgagcag ggtcttgccc gccacctcga
      841 ccagcgattt gggcctgtcg tcggtcaggg gccgcatccg cgtgcccaga ccggcggcca
      901 gaatgaaggc ggtttcgggc gcgccgctca tgccgcgatc cgttccgcga agccgtgacg
      961 cttaaaccag cccagcaggc cctccatgcc cggctggcgc aggttgcggc cgaggtggcg
     1021 ccacatgcgc ggcatgaagg cggcgtagcg cggcttggcg tcgcgcgtga ccagccgcgc
     1081 gaagatgccg aggatgcgcg cctcgttgag cgtcgccagc gcggcgtagt cggccatgaa
     1141 ggcgtcgcgg tcgatctgcg ggcgcaaggc gaaatagtgg tcgagcgcgg cggcctccag
     1201 ctccggcgag acgtcgcggc gcgcgtcctg caacagcgac agcaagtccc aactggggtg
     1261 ggcgcgcagg cagtcctgaa aatcgatcat gccgacgcgg gccgcgccgt cgcgttgcgg
     1321 cagccacaac aggttctcgg cgtggtagtc gcggtggatg aaggccgtgg cgcccgcctc
     1381 ggcgcggcgg cgcaggggcg cccacaacgc ctcccattcg gcggcggcct cgtcgccgat
     1441 gcgcgcgccg gaatcgaact tcgggaacca ttcgatgaac aggtccgcgc cggtcttcag
     1501 cgccagatcg tcgtaggtga gcagcggcca gccgtattcg ccctccggcg acagcacgtc
     1561 gggcggggtc agtgcgtgca gccgcgcctg cgcctcgacg gcggacaggt agagcggcgt
     1621 ctcgtcctga ccgcgctcga tcaggcgcgc gaacaggtcg tcgcccaagt cttcgctgat
     1681 cagcaggccg ttggcggcgt cgacctcgta aatctccggc gccgacaggc cctgcgctcg
     1741 cagatagctg gacgcggcga cgaaggcctc gatccgtccc gccgccagcc gcgccatcgc
     1801 gttgtagccg gaggcgcggc gctcgtcttg cgtctcgtgc aggcggcagg gctggctttc
     1861 cagcgcgggc ggctggtcca tcagcatgaa ggtcgcgcca ttggcctgcg tcagccgctc
     1921 atagcgccgc gtcgaggcgt cgccggggag gggctggcga tgcgcgccgg cgaatccggc
     1981 gctgagcagg aagccttgct tgatcgcttc ccggtcgatg atgtcggtca taaatcgtcc
     2041 ctgaaccggc cgatgggcgt cagggcggcg atgcgcccct gaccggcgtc cggcgaattg
     2101 gtcttgtccg ccatttctag gccgatctcc agccggtcgt caaagcccaa atggccaagg
     2161 cggtccggcc attcgatcag caggaccgag gcgggcgcca gctcgtccag acccagttcg
     2221 taagcctcct ccgggtcggt caggcggtag aggtcgaggt gcagcacgtc gaaatccgcc
     2281 gccgcgtaga tctgcatcag ggtgaaggtg ggcgagggga cgtcctgatc gggcgcggtc
     2341 agggcgcgga tcaggccgcg cgccagcgac gacttgccca tgcccagacc gccgcgcaag
     2401 taaaccacgt cgcccgcgcg caacagcggc gcgatcttcg cgcccagccg cgcggtggcg
     2461 gcttcgtcgg gcaggacgaa gcgttgcggc cgggacaggt cgagcggttc agccatgata
     2521 gggcgcgtcc ggttgctggc gcagcgtggt ttcgacatag gctttcaggc gctccgcgtc
     2581 gagatcgtcc gtgtccggat cgatcggggg cccgaggctg aggtcgaagc gcttgcccgc
     2641 cttgttgaga aattcgtgga acagggtgat gtcgcgcaat tcccgcgaaa agcggttgaa
     2701 caggtggaac cagaccgagt ccggcccgga cagatacgca ggcacgatcg gcgcgccgta
     2761 cttctgcgcc agcgacgcgc aggttccggc ccacggcgga tcgatcagcg cgcccgccgc
     2821 gtcgcgccgc gccagccgtc cggcggggaa catcaccacg caggcttcgc gctcgaaggc
     2881 ggatttcgcg gcctgcaagg tggcgcgggt cttgtcgcgc gtgcgcttgg cctcgaccca
     2941 ctcgaccggg atcagcgatt catcgaagcg cggattgacg cgcagcgcgt cggcgttggc
     3001 gaagaagacg atgtcgtcgc ggcggcgctt caaggcgtcg cgcacggcga cgccgtcggc
     3061 gatgccggtc ggatggttgc acacgacgag gcagcggccg gtttgcggca ggcggtccag
     3121 ccgccgcgcc gcgacctcca gcgccagcag ccgcgacacg taatcgagcg cgtcctcgcc
     3181 gctcaggtcg cggatcgcgt cggccatgcc gaccgccttc ggatagccga gcagcgcata
     3241 gagcggcggc ctgacgaacg gccagaacgg gcttttgacc agcctcggcg cgcgctcgta
     3301 aatcagttcg tcgataatgt gcatgaggtt ctgatctggc gtgatttcgt gagcttggca
     3361 aggcgcgcgc gccggggtaa cctgtcgcca tgaccacgcc cgcgcccaca tccaagcctc
     3421 gtcccgaaag cgaagaccgc cgccatccca aggcgttttt tccgtatctg atcctgtggg
     3481 ccgtctgcct ggcctgcctc gccttcgccg ccgtgatgct gatcgccgct tagggtgatg
     3541 ttttacgcgc atccgggtcc aaaaagtgcg tcacactttt tgggatgcgc tctaaagact
     3601 ttgcgcgaac gccgttaacc ttttatagtc gcgcggctgt gaacgggagg ggctgcggcg
     3661 atgagcagga aagcgctgtt gtgggcgatg gccctggtgg tgatcgtgcc cgccctgatc
     3721 ggcggcggct actggatctg gtgggatcag gtcgagaaat acgcgccggt cgccatcgcc
     3781 aatcccgacg acgcctccgc cattcaatcg cttctggacc gggtcgatta tgtttctccg
     3841 ggaccgcgcg ggcgctgggt ctatctgatc ggctaccgca actgcgcgcc gtgcaacgcc
     3901 tatcagcgca gcgaattcgc gcgcctgcag gcggcggggg tcgagacgcg cgtggtgatg
     3961 ttcgcccggc ccgacaacgg cgcgcagaag caatcgacgc cgcaggagcg ctcgaccgtc
     4021 gccgaactgt ggctgaaccg gagctggccc ctgtacctgc aatggttcaa cgcgcccgaa
     4081 ggcgactgga aggcggtcgg gctgccggtg gccgacgacg acatggcgcg ctccggcgtg
     4141 gtgtgggcgt cgcgcgaatt catcgccgag ttgacgccgg tgctggcgcg caaccgcgtc
     4201 cacgccggtt atcccctggt catctggcgc gacgccaacc gccagcttcg cgtctgcgcc
     4261 tgcgacagcg acaaggccta ccacttcatc cgcgaggact tcggcgtcaa gggcgacctg
     4321 gaacaggccg cgcagaacat catcgacctg cccggcaagc tgatcggcgg aacgtccagt
     4381 gcggcttcgg ccgactatgg gccggacggg caggcgcagt aaggtttttt gggccacgaa
     4441 gggcacgaaa agacacgaaa aaatgcgata tggaaagata aaccacggaa agcacggaaa
     4501 cgatgcgctt tgcgcatcga cacggaaaga tacggatgcg cacgaagggc caagggtgga
     4561 tatggatgac ggcttcgcct cgaaaccttt tctgtccgcg cctttccgtg tcgccggtca
     4621 aaggccggcg tttccgtgtt ttccgtgtta aaatcggtcg ccaccgtcga gtggcgacaa
     4681 catccgcacg cacttttttt cgtgtttttt cgtgcccttc gtggccaaaa attctttaat
     4741 acggcccaaa acaaaaaccc cgccggcatc gccggcgggg tttgttattt cggatcggtc
     4801 agaaacgctt acgcttcggc gtccgggttg ccggaggctt cggcggcggc catctgggcg
     4861 cgcagctcgg cgcccttgga gccgcgcgtc gagttcatcg agcgctcggc gatacgggcc
     4921 gacttgccgc gacggtcgcg caggtaatag agcttggcgc gacgcacgac gccgcggcgc
     4981 ttgacctcga tcgactcgat gttcggcgac aggatcggga acttgcgctc gacgccttcg
     5041 ccgaacgaga tcttgcgcac cgtgaaggtt tcgttgatgc ccgagcccgc gcgcgcgatg
     5101 cagacgcctt cgaaggcctg gacgcgttcg cggtcgcctt ccttgatctt gacgttgatg
     5161 cgcagcgtgt cgccgggctg gaattgcggg aaggtcttgt cgcctttcag cttggcgact
     5221 tcttcggctt cgatctgctg aagcaggttc atgataatag tcctttaagg gcttacttcc
     5281 gtttggaagc gccgtttgcc tgtaagttgg aaagataggc ctcccacaga tcgggccgcc
     5341 gctctttcgt tgtcgcctct ctttgctgct gcttccattg cgccatcttc ttatggtcgc
     5401 cggacaacag cacatcgggg atgtcgaggc cctcgaagct ccgcggtctt gtgaactgcg
     5461 gctcctcgag gagaccatcc tcgaaactct cgcgctccag gctggccgcc gctcccagca
     5521 cgccggggag cagacgcacg accgcctcga tcgccaccat cgccgccgct tcgccgcccg
     5581 ccaggaccgc gtcgccgacg ctgatttcct cgaacccgcg cgcgtccagc acgcgtt
//
LOCUS       DASVLL010000006         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_215329,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000006 DASVLL010000000
VERSION     DASVLL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215329"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VF402_00120"
     CDS             complement(<1..1342)
                     /locus_tag="VF402_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX7798720.1"
                     /translation="MSPQPAPPASVVTQPQYMAAMPKPKGKASETLAFLKPYLWPSER
                     PDLKIQVALALLAMLVGKLLTVATPFAYKWAANAFALKGAHAPGFWPLVMASPLIMVL
                     AYGVARMFSQVFNNIRDGLFSAVGQHAVRGLANRAFVHLHNLSLRFHLQRRTGGLSRV
                     IERGKAGIETIIRLTMMVGIPTVVEFILTAISLWAQFDLIYVAVVALTVVVYIWFSIV
                     ASNMRISIRKEMNEADSDAMSKAVDSLLNFETVKYFGNEALERGRYDRATAMYEKAAI
                     KTYTSLAWLNIGQAVIFSIGMVTVMLMSAWEVAHGTKTIGHFVLVNAFMIQLAIPLNF
                     IGTLYREISTGLIDMEAMFKLLDEPAEVVDASDAKDLIVTDGAVSFKDVVFSYDPDRE
                     ILKGVSFDVPAGKTVAIVGPSGAGKSTISRLLFRFYDVKGGSIEIDGQDIRGVSQ"
     gene            complement(1430..1852)
                     /locus_tag="VF402_00125"
     CDS             complement(1430..1852)
                     /locus_tag="VF402_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerC family mercury resistance protein"
                     /protein_id="HEX7798721.1"
                     /translation="MQAPEAPHKSRWLDFASVGLSGLCVVHCLALPLLAAALPLAGAL
                     ADAPWVHPLLVLIAAPLSLWAVHTSRAWRKAPVVALVALGLVLLALAAFVPALAALDV
                     ALSVTGALSIAAAHLINYLTGRGAHRHGADCACEAAGE"
     gene            1999..2673
                     /locus_tag="VF402_00130"
     CDS             1999..2673
                     /locus_tag="VF402_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX7798722.1"
                     /translation="MRAKPRIDIWSDVVCPFCYIGETALKQALAETGVEAEIVHHAFR
                     LQPGEAPQPVDDMLARKYGLNGAQVEAQQQRVTDMAAQVGLDFHLAGTLSGDTLDAHK
                     LLALAADKGVDLLSVLYRAYFTDNRDIFARETLLGLGEAAGLSRADMQAALSAPGIAA
                     RVAADEQAARQGGVRGVPFFVVSGAGDERFALSGAQPVSAFAEALRRVQPEAAGPVCG
                     PDGCAE"
     gene            2750..>3051
                     /locus_tag="VF402_00135"
     CDS             2750..>3051
                     /locus_tag="VF402_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX7798723.1"
                     /translation="MIRHIVMFNARTPEDRDAVYRGLSVLTQIPQARVLEIAFNEKID
                     QIANDIDIVVYGEFDSLEDFRAYKAHPLYEESIRQVRHLRNIRVAADYTVSSATREL"
BASE COUNT          553 a         1050 c          969 g          479 t
ORIGIN      
        1 cctgcgacac gccgcgaatg tcctgcccgt cgatctcgat gcttccgccc ttgacgtcgt
       61 agaagcggaa caggaggcgc gaaatcgtcg acttgcccgc gccggacggg ccgacgatgg
      121 ccaccgtctt gcccgccggc acgtcgaacg acacgccctt gaggatctcg cggtcgggat
      181 cgtaggaaaa gaccacgtcc ttgaagctga ccgcgccgtc ggtgacgatc aggtctttgg
      241 cgtccgacgc gtccaccacc tcggcgggtt cgtccagcag cttgaacatc gcctccatgt
      301 cgatcagccc ggtcgagatt tcgcggtaca gcgtgccgat gaagttgagc gggatcgcca
      361 actggatcat gaaggcgttg accagcacga aatggccgat cgtcttggtc ccgtgcgcga
      421 cctcccaggc gctcatcagc atcaccgtca ccatgccgat ggagaagatc accgcctggc
      481 cgatattgag ccaggccagc gaggtgtagg tcttgatcgc cgccttttcg tacatcgccg
      541 tggcgcggtc gtagcgcccg cgctccagcg cctcgttgcc gaaatatttg accgtctcga
      601 agttcagcag cgaatcgacc gccttggaca tggcgtcgct gtcggcctcg ttcatctcct
      661 tgcggatcga gatgcgcatg ttggaggcga cgatggagaa ccagatatag accaccaccg
      721 tcagcgccac gaccgcgaca tagatcaggt cgaactgcgc ccacaggctg atcgcggtca
      781 ggatgaactc gaccacggtc gggatgccga ccatcatggt caggcggatg atggtctcga
      841 tgcccgcctt gccgcgctcg atcacccgcg acagcccgcc ggtgcggcgt tgcaggtgga
      901 agcgcaagga caggttatgc agatgcacga aggcgcggtt ggccagcccc cgcaccgcgt
      961 gctgacccac cgccgagaac aggccgtcgc ggatattgtt gaaaacctgc gagaacatgc
     1021 gcgccacgcc gtaggccagc accatgatga gcggcgaggc catgaccagc ggccagaagc
     1081 ccggcgcgtg cgcgcccttc agggcgaagg cgttggccgc ccacttgtag gcgaacggcg
     1141 tcgccacggt cagcagcttg cccaccagca tggccagcag ggccagcgcg acctgaatct
     1201 tcaggtcggg acgctcggac ggccacagat agggcttgag gaaggccagg gtctcggacg
     1261 ccttcccttt cggcttcggc atggccgcca tatattgcgg ctgcgtcacg acggaggcgg
     1321 gcggcgcggg ctgaggactc atgaggggct tttccggtgt gttcggcgaa tgcgccccta
     1381 tatagggacg cccgccgcgc gcgccaatct ttgcggcgca aaaaagccgc tattcgcccg
     1441 ccgcctcgca ggcgcagtcc gcgccgtgcc tgtgcgcgcc gcgcccggtc agatagttga
     1501 tcaggtgcgc cgccgcgatg ctgagcgcgc cggtcacgct gagcgcgacg tcgagcgcgg
     1561 ccagcgccgg cacgaaggcc gccagcgcca gcaggaccag ccccagcgcg accagcgcca
     1621 ccaccggagc cttgcgccac gcgcgcgagg tatgcacggc ccacaggctg agcggcgcgg
     1681 cgatcagcac cagcagcgga tgcacccacg gcgcatcggc cagcgccccg gccagcggca
     1741 gcgccgccgc cagcagcggc agggccaggc aatgcacgac gcacagcccg gacaggccga
     1801 ccgaggcgaa atccagccat ctcgatttat ggggcgcttc gggagcctgc atgcggcctg
     1861 tcctcgtgaa atgcgttggc gatctccaca tatgttataa agtaactttc cgcaagactc
     1921 tcagggtgaa aaatcgcatc ggcctatcgc atcaaaacgt aaccgtccta tatacggttc
     1981 agccggagga gcttcatcat gcgcgccaaa ccccgaatcg acatctggtc cgacgtcgtc
     2041 tgtccgttct gctatatcgg cgagacggcg ctcaaacagg cgctggccga aaccggcgtc
     2101 gaggccgaga tcgtccacca cgccttccgc ctgcaaccgg gcgaggcgcc gcagccggtc
     2161 gacgacatgc tggcgcgcaa atacggcctc aacggcgcgc aggtcgaggc gcagcagcag
     2221 cgcgtcaccg acatggccgc gcaggtcggg ctcgacttcc atctggccgg gacgctgagc
     2281 ggcgacacgc tcgacgcgca caagctgctg gccctggccg ccgataaggg cgtggatttg
     2341 ctgagcgtcc tctatcgcgc ctacttcacc gacaaccgcg acatcttcgc gcgcgaaacc
     2401 ctgctcggcc tcggcgaggc ggcgggcctg agccgcgccg acatgcaggc ggccctgagc
     2461 gcgcccggca tcgccgcccg cgtcgccgcc gacgaacagg ccgcgcgtca gggcggcgtg
     2521 cgcggcgtgc ccttcttcgt cgtctccggc gccggggacg aacgtttcgc cctgtccggc
     2581 gcgcagcccg tatcggcctt cgccgaggcg ctgcgccgcg ttcagcccga ggccgccggt
     2641 ccggtctgcg gtcccgacgg ctgcgccgaa tagggccgaa tagggcttga cgacgacgcc
     2701 tcaggcttta agtgaggccc gtccgttaaa tgtgatacga gtccgccgca tgatccgcca
     2761 tatcgtgatg ttcaacgcca gaacgcctga ggatcgcgac gccgtctatc gcggcctgtc
     2821 ggtgctgacc cagatcccgc aggcgcgcgt actggagatc gccttcaacg aaaagatcga
     2881 ccagatcgcc aacgacatcg acatcgtggt ctatggcgag ttcgattcgc tcgaagactt
     2941 ccgcgcctac aaggcccatc cgctctacga agaatcgatc cgccaggtgc gccacctgcg
     3001 caacatacgc gtcgccgccg actacaccgt ctcgtccgcc acccgcgaac t
//
LOCUS       DASVLL010000007         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_215331,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000007 DASVLL010000000
VERSION     DASVLL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215331"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(630..1415)
                     /locus_tag="VF402_00140"
     CDS             complement(630..1415)
                     /locus_tag="VF402_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX7798724.1"
                     /translation="MSETDQSQRKPPRKGPPLAYIAFAVSVATPAFLLLVMMGVHVRV
                     LSYNLGFMNLTLDLGPKLAMATLAFAGLSLLVSLFMAPLRYGPWALAAVVLSGATLGG
                     YAAYDKALKMYPPIYDVATNWDRPITLSDALLADRGPDALPAPSAPRVARNESVEWGG
                     KTVAEINAETCPAARTFKGKLPSADRIAAMLSADKRYTVFGTAPWRVEATYQDNFYGF
                     KSDIIIRIDPDGIDVRSVGRDNPRDLGGDCRRVVEILNKLKAI"
     gene            complement(1531..>2043)
                     /locus_tag="VF402_00145"
     CDS             complement(1531..>2043)
                     /locus_tag="VF402_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7798725.1"
                     /translation="LGAAHRFLDPAPARLMAIGGLSGSGKSTRARKSAPELGRATGAV
                     ALRSDEIRKRLWACPEFERLPREAYTADENNRVYTQMFNLAETTLRAGQAVVLDATFR
                     EAEWRDRAERLARDCGVGFDGVWLTAAADVRRSRVEARVRDVSDATPDVAAAQGEVDP
                     AALSWRAEAG"
BASE COUNT          389 a          685 c          601 g          368 t
ORIGIN      
        1 atcaagctgt taacggacta aatccttctg tcgtgaatcc gtcaatcggc cgacatattt
       61 ttgtccatat caattgcaac ctgtttcaat aaagtcacag gagcaacctt tacgtagcgt
      121 caaaaaacgc actgtcccgg aagcgtatta tgcccctcgc ccgctttcgc ctcgtatgcg
      181 gtcgttccgc aatttgtcat attaaagtat atacttattt aaagtcttta aatttttatc
      241 cgaggttttt cccgataaga gactcgcgtt ttacggcccc aggcgagaaa tttattgcgc
      301 cgccgccgcc gtcaggcgca ccttcaccaa atttggccca ggcgcacagc ctctctcaat
      361 ccgggcaaaa tgcaccggcg aatcagcgtt acaggcgaaa aatatttcgc agcgggcgcg
      421 gcttcagtgt ttgacgcgaa tatgcgcgcc ctgagacgcg ctggctggcg gcgagggcgg
      481 catcgtcagg acatgtatca aaaccagact cagacacgcg gcggcgcata tctgacagcg
      541 ctttgaaata tataggaaaa tctgaacatt catgataggg ccggaatctg cgcaagccgg
      601 gccgcagcaa aaatcaggcc ataaaaaagt cagatcgctt tcaatttgtt gaggatttcg
      661 accacgcgcc ggcaatcgcc gcccagatcg cgcgggttgt cgcgcccgac cgagcgcaca
      721 tcgatcccgt cgggatcgat acgaatgata atatctgatt tgaaaccgta gaaattatcc
      781 tgatacgtcg cctcgacgcg ccacggcgcc gtcccgaaca ccgtgtagcg cttgtcggcg
      841 ctcagcatgg cggcgatccg gtcggcgctg ggcagcttgc ccttgaaggt gcgcgccgcg
      901 gggcaggttt cggcgttgat ctccgccacc gtcttgccgc cccattcgac cgactcgttg
      961 cgcgccacgc gcggcgcgga cggcgccggc agggcgtccg gcccgcggtc cgccagcagc
     1021 gcgtccgaca gcgtgatcgg ccgatcccag ttcgtcgcca cgtcatagat cggcgggtac
     1081 atcttcaacg ccttgtcgta ggcggcgtag ccccccaggg tcgcgcccga cagcacgacc
     1141 gccgccagcg cccacggccc gtagcgaagc ggcgccatga acagcgacac cagcagcgac
     1201 agccccgcga acgccagcgt ggccatggcc agcttgggcc ccagatcgag ggtcaggttc
     1261 atgaagccga gattatagct cagcacgcgc acatgcaccc ccatcatgac cagcagcagg
     1321 aaggccggcg tggcgacgga aacggcgaag gcgatatagg ccaggggcgg ccccttgcgc
     1381 ggcggtttcc gctggctctg atcggtctcg ctcatgaaac gcctcccttt ccgcccttca
     1441 gggcgcccag attacccctt gaagcgggca aattcacgtt cttaccttca atgcgcgaac
     1501 ttttttcggg cgcgccgaaa tccgccgccc tcaccccgcc tcggcgcgcc acgacagcgc
     1561 ggccggatcg acctcgccct gcgcggcggc gacgtcgggc gtggcgtcgg acacgtccct
     1621 gacgcgcgcc tcgacgcgcg agcggcgcac gtccgccgcc gccgtcagcc acacgccgtc
     1681 gaagccgact ccgcagtccc gcgccagacg ttcggcgcgg tcgcgccatt ccgcctcgcg
     1741 gaaggtcgcg tcgagaacga ccgcctgccc ggcgcgcagc gtcgtttcgg ccagattgaa
     1801 catctgcgta tagacgcggt tgttctcgtc cgccgtatag gcctcgcgcg gcaggcgctc
     1861 gaattcggga caggcccaca ggcgtttgcg gatttcgtcc gaacgcagcg ccacagcgcc
     1921 cgtcgcccgg cccagttccg gcgcgctctt gcgcgcgcgc gtgcttttgc cagagcccga
     1981 caggccgccg atcgccatca gccgcgccgg cgccggatcg agaaagcggt gggcggcgcc
     2041 gag
//
LOCUS       DASVLL010000008         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_465, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000008 DASVLL010000000
VERSION     DASVLL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="zwf"
                     /locus_tag="VF402_00150"
     CDS             complement(<1..1067)
                     /gene="zwf"
                     /locus_tag="VF402_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7798726.1"
                     /translation="MPSHKASAGSRQQVFVLVGGTGDLAMRMLWPSLAMLDQDGFLSE
                     SLRVISVARENISTDVMADRIAEAVKKRIGKDLEDATLPRFRQRITHVALDAARDDWG
                     PKLKAELGDVANLDIVFFLSVSPSLFKPICEHIVTAGLNHAPNRVVIEKPIGRDLESS
                     KVINDSVGHAFEESRIFRIDHYLGKETVQNLIALRFGNTVFEPLWNAQSIDHVQISIS
                     ETVGTGERLGYYDEYGAIRDMLQNHLLQLLCLIAMEPPANLSSDALRDEKVKVLRSLK
                     PMDDVLHKTARGQYGPGFADGERIKGYEAEKGSPSDTETYVAVKAEIANWRWSGTPFY
                     LRTGKCLPSRTTEIVIQFKPVP"
     gene            1694..4309
                     /locus_tag="VF402_00155"
     CDS             1694..4309
                     /locus_tag="VF402_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo 1,3/1,4-beta-D-glucan glucohydrolase"
                     /protein_id="HEX7798727.1"
                     /translation="MSVTHPGARRLTSSCLKALLGAGVALAVLSAGAADARGVHKPVH
                     KAHKTAAKTVRAKSGVIPVDPAWPKLAPAGLVDPATEKRITAIMAKMTLEEKVGQTIQ
                     ADINFIKPADLTKYPIGSILAGGNSSPGGNERASPDQWLQLADDYWRGAAARPTKAPI
                     PLLFGIDAVHGHSNLVGAVIFPHNVGLGAAHDPDLIQKIGEVTADEMSLAGVDWTFAP
                     TVAVARDKRWGRAYESYSENPADVAAYAGRMVEGLQGVNGGKDGVQPGHIMSSAKHFL
                     GDGGTEGGKDQGDAEMSEADLARIHGAGYPPAIQAGVLSVMVSFSSWNGKKMTGNKEL
                     ITGALKQRMHFDGFTVTDWNAQGQVPGCNNGDCPAAMNAGIDMFMAPDEWKAMYENTL
                     KEVKSGVIPQARLDDAVRRILRAKIKGGLFDRAAPKDRAIAGHWENLGSPEHRAVARQ
                     AVRESLVLIKNENHTLPLKPAGHILVTGDGADNIGKQCGGWTITWQGTGNKNSDFPHG
                     ESIYQGVKETVEAAGGTAEVSTDGSYKQRPDAAIVVIGEDPYAEFQGDRANLVYKPGD
                     TSELDMIQKLKAQGIPVVIVFESGRPLWTNPYINAADAFVEAWLPGTEGGGIADVLIG
                     DASGQPRHDFKGKLTFSWPKKGNQGPLNVGTAGYDPLFAYGYGLTYEDVGDVPHLSED
                     SGVSLDDVVNVDDYFDAGRVKAPWRLYLTDDKGQTQGDRDAFASPGGVVSQTAVDADK
                     QEAGRSLVFNGQGHGEAVFTALKPVDLSRQQGGKMTIAVTYRLDKPLQGPVMLGMGAD
                     AGHEHALDVASVLTAPVGQWKTLKVTLDCMANAGVDMKNVGAAFSLSSDKPLAISYSS
                     VKLASDEGDAVCPAK"
BASE COUNT          853 a         1377 c         1450 g          680 t
ORIGIN      
        1 ggcaccggct tgaactggat gacgatctcg gtggtgcgcg acggcaggca cttgccggtg
       61 cgcaggtaga acggcgtgcc ggaccagcgc cagttggcga tctcggcctt gaccgcgaca
      121 taggtttcgg tgtcggaggg cgagcccttc tcggcctcgt agcccttgat gcgctcgccg
      181 tcggcgaagc cggggccgta ctggccgcgc gcggtcttgt gcagcacgtc gtccatcggc
      241 ttcaggctgc gcagcacctt gaccttttcg tcgcgcagcg cgtccgacga caggttggcg
      301 ggcggctcca tcgcgatcag gcacagaagc tgcaacaggt ggttctgcaa catgtcgcgg
      361 atcgcgccgt actcgtcgta atagccgagg cgctcgcccg tgccgacggt ttcggaaatg
      421 ctgatctgga cgtggtcgat cgactgggcg ttccacaacg gctcgaacac cgtgttgccg
      481 aagcgcagcg cgatcaggtt ctgcaccgtc tccttgccga gatagtggtc gatgcggaag
      541 atgcgcgatt cctcgaaggc atggccgacc gaatcgttga tgactttcga gctttccagg
      601 tcgcgcccga tcggcttttc gatcaccacg cggttcggcg catggttcag gcccgccgtg
      661 acgatatgtt cgcagatcgg cttgaacagg gacggcgaca ccgacaggaa gaagacgatg
      721 tcgagattgg ccacgtcgcc cagctcggcc ttgagctttg gcccccagtc gtcacgcgcg
      781 gcgtcgagcg cgacatgcgt gatgcgctgg cggaaacgcg gcagggtggc gtcttcgaga
      841 tccttgccga tgcgcttctt caccgcctcg gcgatgcggt cggccatgac gtccgtgctg
      901 atgttctcgc gcgcgaccga gatgacgcgc agcgactcgc tcaggaaacc gtcctgatcc
      961 agcatggcca gggacggcca caacatgcgc atggccaggt cgccggtgcc gccgacgagg
     1021 acgaaaactt gctggcgcga accagccgag gctttgtgag acggcatagg cgtatttcat
     1081 tcccggacgt tgggcccctt gagacagggc ttatttttga cagcgctgtc ataacccctg
     1141 tcccgcacaa ggtcaatcgt gaaagtggcg ggcgcggaga tttcaggcga tttctccgcc
     1201 atataggccg gagcggcggg gaaagacaac cggtaaacag attttaaggc ggcggcggcc
     1261 cggcgcccgg agcaaaaaaa gcccgatgaa ccgggggtcc atcgggtcta aaggccagtg
     1321 ccttccaaac aatgccacat ttaagaaggc gctttaaaat caccagacgt tccgggccat
     1381 gaaaaaggga aagaaacccg agacgtccga acggcgattt taaacacaaa cccaacgcga
     1441 cggatcgcgc cgggaaacag ggtgagcgtt ctataatccc atctgacaac gctgtcaaac
     1501 aaataataaa gcaaaaagaa cattgagcgc aaaatccagc cgtggcagcg ctcccaaagc
     1561 cttatgcggc ttgacgctgc gtgatagcgc tgtcaatgaa gacggatgcg ccgcccgcat
     1621 agcggcggtc cgcggcgcct cgcgccgcgg acataaaaac gataatgttg tggaagaacg
     1681 caaggaaaag tccatgtccg tcacgcatcc cggcgcgcgc cgtctcacct cgtcctgcct
     1741 gaaagctctg ctgggcgccg gcgtcgcgct ggccgttctg agcgcgggcg cggcggacgc
     1801 acggggcgtt cacaagccgg tccacaaggc gcataagacc gcggccaaaa ccgtcagggc
     1861 gaaatccggc gtgatccccg tcgatccggc gtggccgaag ctggcgcccg ccggtctggt
     1921 cgatccggcg acggaaaagc gcatcaccgc catcatggcg aagatgacgc tggaggaaaa
     1981 ggtcggccag acgattcagg ccgacatcaa cttcatcaag cccgcggacc tgacgaaata
     2041 tccgatcggc tcgatcctgg cgggcggcaa ctcctcgccc ggcggcaatg agcgcgcctc
     2101 gccggaccag tggctgcaac tggccgacga ctactggcgc ggcgcggcgg cgcggccgac
     2161 caaggcgccg atcccgctcc tgttcggcat cgacgcggtg catggccatt ccaacctcgt
     2221 cggcgcggtg atctttccgc acaatgtcgg cctgggcgcc gcgcacgatc ccgacctgat
     2281 ccagaagatc ggcgaggtga cggcggacga aatgtcgctg gcgggcgtgg actggacctt
     2341 cgcgccgacc gtggcggtgg cgcgcgacaa gcgctggggc cgcgcctatg aaagctattc
     2401 ggagaacccc gccgacgtcg ccgcctatgc gggccgcatg gtcgaggggc tgcaaggcgt
     2461 gaacggcggc aaggacggcg ttcagccggg ccacatcatg tcctcggcca agcatttcct
     2521 cggcgacggc ggcacggagg gcggcaagga tcagggcgac gccgagatga gcgaggccga
     2581 tctggcccgc atccacggcg ccggttatcc gcccgcgatt caggcgggcg tcctgtcggt
     2641 catggtgtcc ttctcgtcgt ggaacggcaa gaagatgacc ggcaataagg agctgatcac
     2701 cggcgcgctg aagcagcgga tgcatttcga cggcttcacc gtcaccgact ggaacgcgca
     2761 ggggcaggtg ccgggctgca acaacggcga ctgtccggcg gcgatgaacg cgggcatcga
     2821 catgttcatg gcgcccgacg aatggaaggc gatgtatgag aacacgctca aggaggttaa
     2881 gtcgggcgtg atcccgcagg cgcgcctcga cgacgccgtg cgccgcatcc tgcgcgccaa
     2941 gatcaagggc gggctgttcg accgcgccgc gcccaaggac cgcgccatcg ccgggcattg
     3001 ggaaaacctc ggctcgcccg aacaccgcgc cgtcgcgcgg caggcggtgc gcgaatcgtt
     3061 ggtgctgatc aagaacgaga accacacgct gccgctgaaa ccggcgggcc atatcctcgt
     3121 caccggcgac ggcgccgaca atatcggcaa gcagtgcggc ggctggacca tcacctggca
     3181 gggcacgggc aacaagaatt ccgacttccc gcacggcgag tcgatctatc agggcgtcaa
     3241 ggagacggtc gaggcggcgg gcggcacggc cgaggtcagc acggacggca gctacaagca
     3301 gcgccccgac gccgccatcg tggtcatcgg cgaagaccct tacgccgaat tccagggcga
     3361 ccgcgccaac ctcgtctata agccgggcga cacgtccgag ctggacatga tccagaagct
     3421 gaaggcgcag ggcattccgg tggtgatcgt gttcgaatcg ggccgccccc tgtggaccaa
     3481 cccctatatc aacgccgccg acgcctttgt cgaagcgtgg ctgcccggca ccgagggcgg
     3541 cgggatcgcc gacgtgctga tcggcgacgc cagcggccag ccgcgccacg acttcaaggg
     3601 caagctgacc ttctcgtggc cgaaaaaggg caatcagggg ccgctcaatg tcggcacggc
     3661 gggttacgat cctctgttcg cctacggcta cggcctgacc tatgaggatg tcggcgacgt
     3721 gccgcacctg tcggaggatt ccggggtcag cctcgacgac gtggtcaatg tcgacgacta
     3781 tttcgacgcg ggccgggtca aggcgccgtg gcggctgtac ctgaccgacg acaagggcca
     3841 gacgcaaggc gaccgcgacg ccttcgccag ccccggcggc gtggtgtcgc aaaccgccgt
     3901 cgacgccgac aagcaggagg ccggacgctc gctggtcttc aacgggcagg ggcatggcga
     3961 agcggtgttc accgcgctca agccggtcga cctgtcgcgc cagcagggcg gcaagatgac
     4021 catcgccgtc acctatcgcc tcgataagcc gctgcaaggc ccggtgatgc tgggcatggg
     4081 cgcggacgcg ggccacgaac acgcgctcga cgttgcgtcc gtcctgaccg cgccggtcgg
     4141 gcaatggaag acgctgaagg tcacgctcga ctgcatggcg aacgcgggcg tggacatgaa
     4201 aaacgtcggc gccgccttca gcctgtcgtc cgataaaccg ctcgcgatca gctattccag
     4261 cgtcaaactg gcctccgacg aaggcgacgc ggtctgcccg gcgaagtgac gctcgtctga
     4321 cgagcgaggg ggcgtcttcc ttccacggga acaaaaaaag
//
LOCUS       DASVLL010000009         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_376582,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000009 DASVLL010000000
VERSION     DASVLL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376582"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VF402_00160"
     CDS             <1..284
                     /locus_tag="VF402_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7798728.1"
                     /translation="YNVVDALDQIAQETGKTLPQVALNWLLSRPGVANVIVGARNEEQ
                     LRANLGAIGWSLTPDQIDRLDAASRQYPPYPYWHQMDFDERNPKPTRWG"
     gene            complement(481..>1895)
                     /locus_tag="VF402_00165"
     CDS             complement(481..>1895)
                     /locus_tag="VF402_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7798729.1"
                     /translation="GAGATSGGAMDASNLLKPALAAGTLRCMGSTTYKEYRQHFEKDR
                     ALVRRFQKIDVNEPTLDDTIKILKGLKPYYETFHHLKYTDAAIKSAVELSARYITDRK
                     LPDKAIDVIDEAGALQMVVPESKRKKVIGPKEIEHVISKIARVPSKSVSRDDTQALRD
                     LKTDLQRVVYGQDEAIEQLSTAIKLSRAGLRDPQKPIGCYLFSGPTGVGKTEVAKQLA
                     ATLGIDLLRFDMSEYMERHTVSRLIGAPPGYVGHDQGGLLTDAVDQQPHAVVLLDEIE
                     KAHQDIYNILLQVMDHGQLTDAVGKKVDFRNVVLIMTTNAGASDNQKNSIGFGREKVS
                     GEDEAAIKRLFTPEFRNRLDAIVTFRQLKPEVISQVVQKFILQLEMQLADRHISIELT
                     EDAAKWLAENGYDELYGARPLARVIQENIKKPLADEILFGKLVKGGHVLVKLSDGKIA
                     FDITPAQKGTDKAAAAEAVE"
BASE COUNT          301 a          625 c          599 g          370 t
ORIGIN      
        1 tctacaacgt cgtcgacgcc ctcgaccaga tcgcgcagga aaccggcaaa accctccccc
       61 aggtcgcgct caactggctg ctctcccgcc ccggcgtcgc caacgtcatc gtcggcgccc
      121 gcaacgaaga acaactccgc gccaatctcg gcgccatcgg ctggtccctc acccccgacc
      181 agatcgaccg cctcgacgcc gccagccgcc aatacccgcc ctacccctac tggcaccaga
      241 tggacttcga cgagcgtaac ccgaagccga cgcggtgggg ataagtttgc agggggcaat
      301 cgccccctgc accccgtaac gggttcgcgc caacccgtca ggttttgcgc ctgtccccat
      361 acgaacaaaa atcccgaccc cttggggccg ggatttttgt tcgttcggaa tagacgcaga
      421 agcccgaagg tccgcgcgaa cccgttacgg gggcgcgggg gacaattgtc ccccgcatcc
      481 ttactccacc gcctcggccg cggccgcctt gtcggtgccc ttctgcgccg gggtgatgtc
      541 gaaggcgatc ttgccgtcgc tgagcttgac gaggacgtgg ccgcccttga ccagcttgcc
      601 gaacaggatt tcgtcggcca gcggcttctt gatgttctcc tggatgacgc gggccagcgg
      661 gcgggcgccg tagagttcgt cgtagccgtt ttcggccagc cacttggccg cgtcctcggt
      721 cagttcgatc gagatatggc ggtcggccag ttgcatctcc agttgcagga tgaacttctg
      781 cacgacctgc gagatgacct ccggcttgag ctggcggaag gtgacgatgg cgtcgagacg
      841 gttgcggaat tccggcgtga acaggcgctt gatggccgcc tcgtcctcgc cggacacctt
      901 ttcgcgtccg aagccgatcg agttcttctg gttgtcggac gcgcccgcgt tcgtggtcat
      961 gatcagcacc acgttgcgga agtcgacctt cttgccgacc gcgtcggtca actgaccatg
     1021 atccatgacc tgcagcagga tgttgtagat gtcctgatgc gccttctcga tttcgtcgag
     1081 cagcacgacc gcgtgcggct gctggtcgac ggcgtcggtc aggaggccgc cctgatcgtg
     1141 gccgacatag cccggagggg cgccgatcag ccgcgaaacg gtgtggcgct ccatatattc
     1201 cgacatgtcg aagcgcagca ggtcgatgcc cagcgtcgcc gccagttgct tggccacctc
     1261 ggtcttgccg acgccggtgg ggccggagaa caggtagcag ccgatcggct tttgcggatc
     1321 gcgcaggccc gcccgcgaca gcttgatcgc ggtggacaac tgctcgatgg cctcgtcctg
     1381 accatagacg acccgttgca ggtcggtctt gaggtcgcgc agcgcctgcg tgtcgtcgcg
     1441 cgacacggat ttggacggca cgcgggcgat cttggagatc acatgctcga tctccttggg
     1501 gccgatgacc ttcttgcgct tcgattccgg cacgaccatc tgcaacgccc cggcttcgtc
     1561 gatcacgtcg atcgccttgt cgggcagctt gcggtcggtg atgtagcgcg ccgacagctc
     1621 gaccgccgac ttgatggcgg cgtcggtgta tttcaggtgg tggaaggtct catagtaagg
     1681 cttgaggccc ttcaggatct tgatggtgtc gtccagggtc ggctcgttga cgtcgatctt
     1741 ctggaagcgc cggaccagcg cccggtcctt ctcgaagtgc tgacggtact ccttgtaggt
     1801 ggtcgagccc atgcagcgca gcgtgcccgc cgccagagcc ggtttcagca ggttggaggc
     1861 gtccatcgcc ccgccggagg tcgcgccggc gccga
//
LOCUS       DASVLL010000010         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_323130,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000010 DASVLL010000000
VERSION     DASVLL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323130"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VF402_00170"
     CDS             complement(<1..680)
                     /locus_tag="VF402_00170"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX7798730.1"
                     /translation="MTVLIDVLKDQQRSENGQRAADLSARYQGWSAQDILRDAIRNQF
                     FGQIALSSSFGADSAALLHIAAQIDPNLPVIFLDTERHFFQTLQYRDELAGRLGLSNL
                     INLRADADEAAAEDGKGTLWRTDPDACCALRKVRPLAREMETYAAWISGRKRHQSATR
                     AALPIVEWDGRHFKVNPLAAWTADDLAAYFQAHDLPPHPLVEQGFPSIGCFTCTKPVA
                     AGEDARSGR"
     gene            complement(693..1169)
                     /locus_tag="VF402_00175"
     CDS             complement(693..1169)
                     /locus_tag="VF402_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX7798731.1"
                     /translation="MSRPNSSYEPQLVNRAGDVLGDDGIETLDFERAVSELPTLNGSC
                     AVRVEPAQDVRELLPYLEHIARFDIVFPGYRDGRGYSSARILRDAGYKGALRAVGDVL
                     RDQVFIMLRCGFDEFLLKDRTPSETVVAAAKRYSVTYQSAADAATPVWALRRAGAV"
     gene            complement(1156..>2766)
                     /locus_tag="VF402_00180"
     CDS             complement(1156..>2766)
                     /locus_tag="VF402_00180"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX7798732.1"
                     /translation="IRDRVADFSDQVARRLSGEITEDQFKPLRLMNGLYLQLHAYMLR
                     IAIPYGLLAPRQMRRFAHIARTYDRGFGHFTTRTNLQFHWIKLAETPQILTELAEVEM
                     HSLQTSGNCIRNTTSDHFAAAAKDEFEDPRPWCELIRQWSSNHPEFSFLPRKFKIAIT
                     AAVKDRAAIRLHDIGLHLTPQGFDVYVGGGMGRTPALAHCIRRGLPGSRLLGYLEACL
                     RVYNRYGRRDNSFKARIKILVNALGPDEYKRQVEAEWATMDASRIDPPSDEIERIRAF
                     FPLPDLSDAAFDAAAHARAETADPGFARWVKNNTHPHKRDNHVSVTISLKPIGGTPGD
                     ASADQMDVMADMAETYGYNELRITHEQNVVLPHVARADLYALYRRLDAAGLGTANAGK
                     ITDIICCPGLDYCSLANARSIPLAQEISKRFEDARLSDKIGDLQIKMSGCINACGHHH
                     VGHIGILGVDKQGVEFYQILLGGRADEKAALGVITGKGLPAEAVPGAVERVAQLYLKL
                     RKDDNERFIDTLERLGREAFAEALHEPA"
BASE COUNT          432 a          857 c         1005 g          472 t
ORIGIN      
        1 cggccggagc gcgcgtcctc gcccgccgcg accggcttgg tgcaggtgaa gcagccgatg
       61 gacggaaagc cctgctcgac cagcggatgc ggcggcaggt cgtgggcctg gaaataggcg
      121 gcgaggtcgt ccgccgtcca cgcggccagc gggttgacct tgaaatggcg cccgtcccac
      181 tcgacgatcg gcagggcggc gcgggtcgcg ctctgatggc gcttgcggcc cgaaatccag
      241 gcggcgtaag tctccatctc gcgcgccagc gggcgcacct tgcgcaaggc gcagcaggcg
      301 tcgggatcgg tgcgccacag cgtgcccttg ccgtcctccg ccgccgcctc gtccgcgtcg
      361 gcccggaggt tgatcaggtt gctcaggccg aggcgtccgg ccagctcgtc gcggtattgc
      421 agcgtctgga agaagtggcg ctcggtgtcg aggaagatca ccggcaggtt cggatcgatc
      481 tgcgcggcga tgtgcagcag cgccgccgaa tccgcgccga aggacgagga cagggcgatc
      541 tggccgaaga actggttgcg gatggcgtcg cgcaggatgt cctgcgcgct ccagccctga
      601 tagcgggcgc tgagatcggc ggcgcgctgg ccgttctccg acctttgctg gtctttcaga
      661 acgtcaataa ggacggtcat ggcaacagct cctcagaccg cgcccgcgcg ccgcaaagcc
      721 cagaccggcg tggcggcgtc ggcggcgctc tgataggtga cggaatagcg tttggccgcc
      781 gcgacgacgg tttcggacgg ggttctatcc ttgagcagga actcgtcgaa gccgcagcgc
      841 agcatgatga acacctgatc gcgcaacacg tcgccgaccg cgcgcagcgc gcctttgtaa
      901 ccggcgtcgc gcaggatgcg cgcgctggaa tagccgcgcc cgtcgcgata gccgggaaac
      961 acgatgtcga aacgcgcgat atgttcaaga tagggcagca gttcgcgcac gtcctgcgca
     1021 ggctcgacgc gcacggcgca ggagccgttc aacgtgggca gttccgaaac ggcgcgctcg
     1081 aagtcgagcg tctcgatccc gtcgtcgccc agcacgtcgc ccgcgcggtt caccagttga
     1141 ggctcataag aggaattagg ccggctcatg cagggcctcc gcaaacgctt cgcgacccag
     1201 acgttcgagc gtgtcgatga accgctcgtt gtcgtccttg cgcagtttca ggtaaagttg
     1261 cgccacgcgc tcgacggcgc ccggcacggc ttcggcgggc aggcccttgc cggtgatcac
     1321 gcccagcgcc gccttttcgt cggcgcggcc gcccagcagg atctggtaga attccacgcc
     1381 ctgcttgtcg acgcccagga tgccgatgtg gccgacatgg tggtggccgc aggcgttgat
     1441 gcagcccgac atcttgattt gcaggtcgcc gatcttgtcg ctgaggcgcg cgtcctcgaa
     1501 gcgtttggaa atctcctgcg ccagcggaat cgagcgggcg ttggccagcg agcagtagtc
     1561 gaggccgggg cagcagatga tgtcggtgat cttgcccgca ttggccgtgc ccagacccgc
     1621 cgcgtcgagg cggcggtaaa gcgcgtacag gtcggcgcgc gcgacgtgcg gcagcacgac
     1681 gttctgctcg tgcgtgatgc gcagctcgtt atagccgtag gtttcggcca tgtcggccat
     1741 gacgtccatc tggtcggcgc tggcgtcgcc cggcgtgccg ccgatcggct tgagcgagat
     1801 ggtgaccgag acgtggttgt cgcgcttgtg cggatgggtg ttgttcttca cccagcgcgc
     1861 gaagcccgga tcggcggttt cggcgcgcgc atgggcggcg gcgtcgaacg cggcgtccga
     1921 caggtccggc agcgggaaga aggcgcggat gcgctcgatc tcgtccgagg gcggatcgat
     1981 gcgcgacgca tccatcgtcg cccattcggc ttcgacctga cgcttgtatt cgtcggggcc
     2041 gagcgcgttg accagaatct tgatgcgcgc cttgaagctg ttgtcgcggc ggccgtagcg
     2101 gttatagacg cgcaggcacg cttcgagata gccgagcaac cggctgcccg gcaggccgcg
     2161 gcggatgcag tgggccaggg ccggggtgcg gcccatgccg ccgccgacat agacgtcgaa
     2221 gccctgcggc gtcaggtgca ggccgatgtc gtgcaggcgg atggcggcgc ggtccttgac
     2281 ggcggcggtg atggcgatct tgaacttgcg cggcaggaag gagaattccg ggtggttcga
     2341 cgaccattgc cggatcagct cgcaccacgg gcgcggatcc tcgaactcgt ccttcgcggc
     2401 ggcggcgaaa tggtccgagg tggtgttgcg gatgcagttg cccgaggttt gcaggctgtg
     2461 catctcgacc tcggccagct cggtcaggat ctgcggcgtt tccgccagct tgatccagtg
     2521 gaattgcagg ttggtgcgcg tggtgaagtg gccgaaacca cggtcgtagg tgcgcgcgat
     2581 atgggcgaag cggcgcatct ggcgcggcgc cagcaggccg taggggatcg cgatgcgcag
     2641 catgtaggcg tgcagttgca ggtacaggcc gttcatcagc cgcagcggct tgaactgatc
     2701 ctcggtgatc tcgccggaca ggcggcgcgc gacctgatcg gagaagtcgg cgacacggtc
     2761 gcggat
//
LOCUS       DASVLL010000011         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_54448, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000011 DASVLL010000000
VERSION     DASVLL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54448"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..1749)
                     /locus_tag="VF402_00185"
     CDS             complement(376..1749)
                     /locus_tag="VF402_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7798733.1"
                     /translation="MSGTTNLFVDVPPEDPRKLSPEARRRHFKEIYARFTAEEARRQA
                     SRCAQCGVPYCQHGCPLQNNIPDWLRLTAEGDFHTAWVLASQTNTLPEICGRICPQDR
                     LCEGACTLEASGWRHVTIGAVERFLGDLAWEEGWILPVEAQARTGQSVGVIGGGPAGL
                     AAVDRLLHAGYDVVVYDRHDKAGGLLTYGIPSFKLEKDVIGRRVERLAQSGARFVLNA
                     EVGTAPAFADLQARHDALLIATGVYRARQLELPGSELGGIAPALDFLIAANRQNLGYT
                     LDAATLALRTATDRDVVVIGGGDTAMDCVRTAVRQKARSVTCLYRKQRADMPGSAREI
                     AHAEEEGVRFLWRSQPTRYLGAGGRIAAVEVAGADAPLKADLVIEAIGFEAEDLPVLF
                     GQPELKIRRNGTLDVAPGTGRTSLPGVFAAGDIVRGASLAVWAVRQGQDAAAAIHAYL
                     SRMRQAA"
     gene            complement(1827..1964)
                     /locus_tag="VF402_00190"
     CDS             complement(1827..1964)
                     /locus_tag="VF402_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798734.1"
                     /translation="MAIRFEHAFGVKADTLMRMQTRYELARAREHEGDLAVEDLTAAS
                     A"
     gene            complement(2028..2618)
                     /locus_tag="VF402_00195"
     CDS             complement(2028..2618)
                     /locus_tag="VF402_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7798735.1"
                     /translation="MRLSREERRSRILNAAIACATRSGFRGASMDEIAREAGISVGVI
                     YRHFKCKEAIIEAIVARDLAELQNKIAGIGELPPRELLALFAADVPGFVQRQREPARA
                     ALRLEIYAEAARNPRVEAILRGFTEAERDIGRQFFRQTLQRPATHAELTARADMLRVI
                     ADGLLASGLYAGDNDAAFVTVLAEVMRRLLTDPART"
     gene            2823..3932
                     /locus_tag="VF402_00200"
     CDS             2823..3932
                     /locus_tag="VF402_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7798736.1"
                     /translation="MAAAVALAALLSACGPKPGGMPQQPAQVGVEIVKTQAVPLTTEL
                     SGRTSSFLVSDVRPQVGGIIKARLFTEGALVKAGQPLYQIDPATYQAALNSAQAALAN
                     AEAALTSAKLKADRYAELVKINAISKQDNDDAQAALQQAQAQVLSAKAQVQSAQINLG
                     YTTVRAPISGRIGKSSYTPGALVTASQADALTTINESDKVYVDISRSSADVLAMKQAI
                     QSGSLGAADSAQVQLILDNGTVYPVTGHLEFSDVTVDEGTGTVNLRAVFDNPNGLLMP
                     GMYVRARINTGVAPNGVLLDPAAVQRDPKGNASVYVVGADNKVAVRPVTLGAGVGQKW
                     LVLSGLNAGDKVIVQGLQKIAPGAPVKAAVVASAE"
     gene            3942..>4742
                     /locus_tag="VF402_00205"
     CDS             3942..>4742
                     /locus_tag="VF402_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7798737.1"
                     /translation="MLSRFFIDRPIFAWVVAIVIMLAGALAILRLPVEQYPDIALPQV
                     TVSASYPGASAKTVEDSVTQVIEQSMKGLDGLQYMSATSTSTGGASLTLTFKSGTDPD
                     IAQVQVQNKLQTVMALLPQSVQAQGVSVVKSTSNFLLVVGVYADDGKTTQTDISDYIG
                     SSIKDQLSRVDGVGDVQLFGAQYAMRIWLDPQKLASYNLMPSDVMAAIQAQNTQVSAG
                     ELGGLPAVPGTMLNATITAQSRLTTPEQFRNIIVKTQSDGAKVYLSDVA"
BASE COUNT          790 a         1672 c         1545 g          735 t
ORIGIN      
        1 ctgcgccctg ctgcgcaaga ccgacccggc ggcggcgcgg ccgctcaacg ccttcctgca
       61 ccgcgcgctg ctgcgccccc gccaagaaaa ttgaagcggg acattaaagc ctggcggcgc
      121 ggcgcagatc gtcgttcaac cggctctgta ataggccagc caatcgggat cgagacgaat
      181 gggcatctgc tggcgtggat taaggaaggg attcaaccac ggacgatggc cttcgtccat
      241 gttttggctg cgccgaactc cgtttccgtg gttaaatccc ttaattcttg caatctgcgt
      301 aatctgcgga cgccccttcc ctccaatggg taggaggcgg caggcagccg tatgatcgtt
      361 ggatgtcgat ccggcctaag ccgcctgccg catccggctc agataggcgt ggatcgccgc
      421 cgccgcgtcc tggccctggc gcaccgccca caccgccagc gaggcgccgc gcacgatgtc
      481 gcccgccgcg aacacgccgg gcagcgaggt gcggccggtt ccgggcgcga cgtccagcgt
      541 gccgttgcgg cggattttca gctccggctg gccgaacagg acaggcagat cctcggcctc
      601 gaagccgatc gcctcgatga ccaggtccgc cttgagcggc gcgtccgcgc ccgcgacctc
      661 gacggcggcg atacgccccc cggcgccgag atagcgcgtc ggctggctgc gccacaggaa
      721 gcgcacgccc tcctcctcgg cgtgggcgat ctcccgcgcc gagccgggca tgtcggcgcg
      781 ctgcttgcgg tagaggcagg tcacgctgcg cgccttctgg cgcaccgccg tgcgcacgca
      841 gtccatcgcc gtgtcgccgc cgccgatgac gacgacgtcg cggtccgtgg cggtcctgag
      901 cgccagcgtg gcggcgtcga gcgtatagcc gagattttgc cggttggccg cgatcaggaa
      961 gtcgagcgcg ggcgcgatcc cgcccagctc cgaccccggc agctccagtt gccgcgcgcg
     1021 atagacgccg gtggcgatca gcagggcgtc gtggcgcgcc tgcaagtcgg cgaaggccgg
     1081 cgccgtcccg acctcggcgt tcagcacgaa gcgcgccccg ctttgcgcca gccgctcgac
     1141 gcgccgcccg atcacgtcct tctccagctt gaacgacgga atgccatagg tcaaaagccc
     1201 gcccgccttg tcgtggcggt catagacgac gacgtcgtaa ccggcgtgca gcagccggtc
     1261 gaccgccgcc agcccggccg ggccgccgcc gatcacgccg accgactggc cggtgcgcgc
     1321 ctgcgcctcg accggcagga tccagccctc ctcccacgcc agatcgccga gaaaacgctc
     1381 gaccgcgccg atggtgacat gccgccagcc cgacgcctcc agcgtgcagg cgccttcgca
     1441 caggcggtcc tgcggacaga tgcggccgca gatttccggc agggtgttgg tctggctggc
     1501 cagaacccag gcggtatgga aatcgccctc ggcggtcagc cgcagccagt cgggaatatt
     1561 gttctgcaac ggacagccgt gctggcaata gggcacgccg cattgcgcgc agcgcgacgc
     1621 ctgacggcgc gcctcctccg ccgtgaaacg ggcgtaaatc tccttgaaat gccgccgccg
     1681 cgcctccggc gacagcttgc gcggatcctc gggcggaaca tcgacgaaca ggttggtcgt
     1741 accggacatg acagggcttc cgcttaaaca tgcattacaa atgcatattt aacaccgccc
     1801 ctcgcccgcc gtaaactgcg acgaaatcac gcagacgcgg cggtcaaatc ctcaaccgcc
     1861 agatcgccct catgctcgcg cgccctggca agctcatagc gagtctgcat gcgcatgagc
     1921 gtgtcggcct tgacgccgaa ggcgtgttca aagcggatcg ccatctccgc ggacagagag
     1981 gcccgcccgt taagcaccgc gccggcctga cgccgccgcc agaccgccta tgtccgggcc
     2041 ggatcggtca gcagccgccg catgacctcg gccagcaccg tgacgaaggc ggcgtcgttg
     2101 tcgccggcgt agaggccgct ggccagcagg ccgtcggcga tgacgcgcag catgtcggcg
     2161 cgggcggtca gttcggcgtg cgtcgccggg cgttgcaggg tctggcggaa gaattgccgc
     2221 ccgatgtcgc gctcggcctc ggtgaagccg cgcaggatcg cctcgacgcg cggattgcgc
     2281 gccgcctcgg cgtagatttc cagccgcaaa gcggcgcggg cgggctcccg ctgacgctgg
     2341 acgaagcccg gcacgtcggc ggcgaacagg gccagcagct cccgcggcgg cagctcgccg
     2401 atcccggcga tcttgttctg caactcggcc agatcgcgcg ccacgatggc ctcgatgatc
     2461 gcttccttgc atttgaaatg gcggtagatg acgccgacgc tgatgcccgc ctcgcgcgcg
     2521 atctcgtcca tgctggcgcc gcgaaagccc gaacgggtcg cgcacgcgat cgccgcgttc
     2581 aggatgcggc tgcggcgctc ctcgcgcgaa agacgcatcg tcgtctcgtt tcgcgtttgc
     2641 gttgatcgcg tcattgtgat gatactgacg gttcctgcat tgcaatatct taataattct
     2701 ccgcttgacc tagcataggc cccccactaa gggaagccac agttgagtga acgttcattc
     2761 tcatttgccg ccttcgacga gtccgcccga tgatcgcccc ttcccgcctc cctctccgtc
     2821 acatggccgc cgccgtcgcg ctggccgctc tcctgagcgc ctgcggcccc aagcccggcg
     2881 gcatgccgca gcaaccggcg caggtcggcg tcgagatcgt caagacgcag gccgtgccgc
     2941 tgacgaccga gctgagcgga cgcacctcgt ccttcctcgt ctcggacgtg cgcccgcagg
     3001 tcggcggcat catcaaggcg cgcctgttca ccgagggcgc gctggtcaag gcgggccagc
     3061 cgctgtacca gatcgacccg gcgacctatc aggcggcgct gaacagcgcg caggcggctc
     3121 tggccaacgc cgaggcggcg ctgacctcgg cgaagctgaa ggccgaccgc tacgccgagc
     3181 tggtcaagat caacgcgatt tccaagcagg acaatgacga cgcccaggcc gccctgcaac
     3241 aggcgcaggc gcaggtgctg tcggccaagg cgcaggtgca gtcggcgcag atcaatctcg
     3301 gctacaccac ggtgcgcgcg ccgatctccg gccgcatcgg caagtcgagc tatacgcccg
     3361 gcgcgctggt cacggccagt caggccgacg ccctgaccac gatcaacgaa tccgacaagg
     3421 tctatgtcga catcagccgc tcgtccgccg acgtgctggc catgaaacag gcgatccagt
     3481 ccggttcgct gggcgcggcg gacagcgcgc aggtgcagtt gatcctcgac aacggaaccg
     3541 tctatccggt caccggccat ctggagtttt ccgacgtcac ggtcgacgaa ggcaccggca
     3601 cggtcaatct gcgcgccgtg ttcgacaatc cgaacgggct tttgatgccg ggcatgtacg
     3661 tgcgcgcccg catcaacacc ggcgtggcgc cgaacggcgt gctgctggac cccgccgccg
     3721 tgcagcgcga cccgaagggc aacgcctcgg tctatgtcgt cggcgcggac aacaaggtcg
     3781 ccgtgcggcc cgtcaccctc ggcgcgggcg tcggccagaa atggctggtc ctcagcgggc
     3841 tgaacgcggg cgacaaggtc atcgtgcagg gcctgcaaaa gatcgcgccg ggcgctccgg
     3901 tgaaagccgc cgtcgtcgcc agcgccgaat aggaagccgc catgctctcg cgtttcttca
     3961 tcgaccggcc gatcttcgcc tgggtcgtcg ccatcgtcat catgctggcg ggcgcgctgg
     4021 ccatcctgcg cctgccggtc gagcagtatc ccgacatcgc cctgccgcag gtcacggtgt
     4081 cggccagcta tccgggcgcc tcggccaaga cggtcgagga cagcgtcacc caggtcatcg
     4141 agcagagcat gaaggggctg gacgggctcc agtacatgag cgccacctcg acctcgaccg
     4201 gcggcgccag cctgaccctg accttcaagt ccggcacgga tcccgacatc gcgcaggtgc
     4261 aggtgcagaa caagctgcag accgtcatgg ccctgctgcc gcagtcggtg caggcgcagg
     4321 gcgtgtcggt cgtgaaatcg acctccaact tcctgctggt cgtcggggtg tacgccgacg
     4381 acggcaagac gacccagacc gacatttccg actatatcgg atcgtcgatc aaggaccagt
     4441 tgtcgcgcgt cgacggcgtc ggcgacgtgc agttgttcgg cgcgcaatac gcgatgcgca
     4501 tctggctcga cccacagaag ctggccagct acaacctgat gccgtccgac gtgatggcgg
     4561 cgattcaggc gcagaacacg caggtgtccg cgggcgagct gggcggcctg cccgccgtgc
     4621 cgggcaccat gctcaacgcc accatcacgg cgcagtcgcg cctgaccacg ccggagcagt
     4681 tccgcaacat catcgtcaag acgcagagcg acggcgccaa ggtttacctg agcgacgtgg
     4741 cg
//
LOCUS       DASVLL010000012         6059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_54484, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000012 DASVLL010000000
VERSION     DASVLL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54484"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3483)
                     /locus_tag="VF402_00210"
     CDS             complement(<1..3483)
                     /locus_tag="VF402_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX7798738.1"
                     /translation="MARKPPKKPLRLDDAPETSPETPPEETPVEQIKAAAETLARKAG
                     FRLSDIRWGRVFRRTAAIAAAILLLLTAFLAALNTPAGRRLLVQFATGIKLNSGLQFQ
                     IDRIDGSLYGKMTIHGLRVVDTKGVFIEAPVVNLDWRPFGYLNKHVDIRDLDAPRIEV
                     LRQPVLNPSQEPEKSGPILPDLRIDLNRVQVGALILDAPVAGQPYTLRFGGAAHILRG
                     RAQAALEALSDKGDRLTLRLDAVPDANKLDLEAHALAPEDGAADRLLGLGKPLSLDLD
                     GKGGWALWRGRLVTEFGGAALSDLQLTAQNGVFHIAGPTHPDLIAGADAAALLRPQLN
                     IDATAKMKDRVFDLNLALASQAVDVKALGVVDLGHNRFRKLEAHARLLRPDALGKDVS
                     ADGLRADLVFDGAFETPRIDYDIAAKRFGYGDIRLSGLDAQGRSHKDGAAIIIPVKAK
                     LDSLSGLGEQVDPLLTHLKLSGELSFDNGKIASKTLKLQSDRLKATGTVSGDVGKGAY
                     AADAQVALNHYTVADLATLNASTRVKVAYGKAGLSAAGTLNARTTKILNDGLAKALGG
                     DASLSGGYAYAPNGAIAVRGLNLKAPKLTMKGDGGLSKSGALSFKASAESADYGPADL
                     TLSGTFDHLQGALHAARPGLGVGVVDLAATFEGVDGGYAVKAAGGSDYGPFSADARIG
                     LDAPLSVDIHHAEFAGIGMAGKLTQSPAGPFTGTLALSGAGLNGSAQLSAAGADQAAA
                     LHAVGNAFTVPGPTPVRIGRALIDASLVLRDQAQIDADVQMANVAYDGVSLATGRAKI
                     ALVGQNGTVQAVANGASGGVPVNLAANAQLAPNLIRVAAQGMANGVAFHTQAPARIAR
                     SGEDWVLSPATVVMDAGRLDVAGRFGASKQAQIRLHDLDLSVANLFQGDLGVTGTANG
                     AIDIKQTGDATPYAHATLKISRFSRASAAVASAPVDMEIEAGLDPDALVRDNYVRAVI
                     RQNGGVIGRVQLGLTPDGGGDWTHRLLNGALSGGVRYNGPAAVPFSLAGLPRQQLTGA
                     VALAADVSGKAADPQLNGVVKADSLTYDNESLGTSITQIALSGRFTSNRLELDSFTGR
                     AGDGTVSASGWLSLAADQHFPMALHADLKNARLARSDAINSVVSGALDVTNDAQNGPL
                     IKGDLRLPQFKYQVV"
     gene            complement(3494..5581)
                     /locus_tag="VF402_00215"
     CDS             complement(3494..5581)
                     /locus_tag="VF402_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7798739.1"
                     /translation="MTGAATGLAMALLAHGAAFAQAAAPAQANPPIITDQAFDAALPP
                     LDAAPPTQVKTQAPQTQASQTQAPQTQAPPTPEQPAEPDALPPVGASDDPDLTAPLPP
                     LQGYDVQPQQAAAPAEAAPEIRYVTQVAGLKAVGVAAEFKALSALENGKGRAANVAMV
                     RARASEDEALILRLLNSRGYFDGAVTTTLKPAPDANGPVTASLVAVPGPLYRFDLIRI
                     DANPTQPPDLIRNALPLTQGDPILADAVLAAEASVSVGMQENGYPFAKIGARDIALDP
                     ADATGDYTLHVDTGDRSRFGGFRSDGKLAFSPKHVGELTRFSRGDLYDNRKVNDLREA
                     LVATGLFRSVSVEPVATGEKAPDGTSYVDLLVHQQAGPVHTLAGEIGYSTGEGFTATG
                     SWQNRNKFPPEGALILTGALGTQQQSLTTTLRRSNAQKRDRTVQLSASLSHNIYDSYE
                     AYAAGLSGSISRVSTPLWQKVWTWSYGFNLEASRESNDLNHNAGTVWQNYYYLDLPVK
                     IGYDRSNDLLNPTTGFRLTSQSTPQISLSAGGSNLSEIFDASYYHPVGAKLVIAMRGR
                     IGTLIGGGLASIAPSRRLYAGGGGSVRGYGYQALGPVDAKGNPTGGLSVVEASLEARY
                     RIGDIGVVPFIDAGQAYASTTPSFSDLRFGAGIGARLYTNFGPIRIDVATPINRHKGD
                     PIVALYVGIGQSF"
     gene            5818..>6059
                     /locus_tag="VF402_00220"
     CDS             5818..>6059
                     /locus_tag="VF402_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX7798740.1"
                     /translation="MTHAVPSAVEADPRWRAVLRRDAADFVYGVMTTGVYCRPSCPSR
                     RPKPENVAFFDSCDAAEAAGLRACLRCRPRGEDPEAR"
BASE COUNT          824 a         1983 c         2243 g         1009 t
ORIGIN      
        1 gaccacctga tatttgaact gcggcaggcg caggtcgccc ttgatcagcg ggccgttctg
       61 cgcgtcgttg gtcacgtcga gcgcgcccga caccaccgaa ttgatcgcgt cggagcgcgc
      121 gaggcgggcg ttcttcaggt cggcgtgcag cgccatcggg aaatgctggt ccgccgccag
      181 gctgagccag ccggaggcgc tgaccgtccc gtcgcccgcg cgcccggtga agctgtccag
      241 ctccagccgg ttggaggtga agcgcccgct cagggcgatc tgggtgatgc tcgtgccgag
      301 gctttcgttg tcgtaggtca ggctgtcggc cttgaccacg ccgttcagtt gcgggtcggc
      361 tgccttgccc gacacgtcgg cggccagagc gacggcgccg gtcagttgct gacgcggcag
      421 cccggccagc gagaacggca cggcggcggg accgttatag cgcacgccgc cgctcagcgc
      481 gccgttcagc agacggtgcg tccagtcgcc gccgccgtcg ggcgtcaggc ccagttgcac
      541 gcgcccgatc acgccgccgt tctggcggat cacggcgcgg acatagttgt cgcggaccag
      601 cgcgtcgggg tccagcccgg cctcgatctc catatcgacc ggcgccgagg cgacggcggc
      661 ggaggcgcgc gagaagcggc tgatcttcag cgtggcgtgg gcgtagggcg tcgcatcgcc
      721 ggtctgtttg atatcgatgg cgccgttggc cgtgccggtg acgccgagat cgccctggaa
      781 caggttggcc accgacaggt cgagatcgtg caggcgaatc tgcgcctgct tgctggcgcc
      841 gaagcggccc gccacgtcca gcctgcccgc gtccatgacc acggtggcgg gcgacagcac
      901 ccagtcctcg cccgacctgg cgatgcgggc gggcgcctgc gtgtggaagg cgacgccgtt
      961 ggccatgccc tgcgccgcca cgcggatcag gttgggcgcc agttgcgcgt tggcggccag
     1021 attgaccggc acgccaccgc tggcgccgtt ggcgacggcc tgaaccgttc cgttctggcc
     1081 gaccagcgcg atcttggcgc ggcccgtggc caggctgacg ccgtcgtagg cgacattggc
     1141 catctgcacg tcggcgtcga tttgggcctg atccctgagg acgaggcttg cgtcgatcag
     1201 ggcgcggccg atgcggaccg gcgtcgggcc gggcacggtg aacgcattgc cgacggcgtg
     1261 cagcgccgcc gcctgatccg cgcccgccgc gctcagttgc gcgctgccgt tcagacccgc
     1321 gccggacagg gccagcgtcc ccgtaaacgg cccggccggg ctttgcgtca gcttgcccgc
     1381 catgccgatc ccggcgaatt cggcgtgatg gatgtcgacg ctgagcgggg cgtcgaggcc
     1441 gatccgcgcg tcggcgctga acgggccgta atcgctgccg cccgccgcct tgaccgcata
     1501 gccgccgtcg acgccctcga acgtggcggc gaggtcgacc acgccgacgc ccagtcctgg
     1561 cctggcggca tgcagcgcgc cttgcagatg gtcgaacgtc ccggacaggg tcaggtcggc
     1621 ggggccgtaa tcggcggact cggcgctggc cttgaagctc agcgcgccgg atttcgacag
     1681 gccgccatcg cccttcatgg tcagcttcgg cgccttgagg ttgaggccgc gcaccgcgat
     1741 cgcgccgttc ggggcatagg catagccgcc gctcaggctg gcgtcgccgc ccagcgcctt
     1801 ggccagtcca tcattgagaa tcttggtcgt gcgcgcgttc agcgtcccgg ccgcgctcag
     1861 cccggccttg ccgtaggcga ccttcacgcg cgtcgaggcg ttgagcgtgg ccagatccgc
     1921 cacggtgtaa tgattgagcg cgacctgcgc gtcggcggcg taggcgccct tgccgacatc
     1981 gccgctgacc gtgcccgtgg ccttgaggcg gtcggactgg agtttcagcg tcttgctggc
     2041 gatcttgccg ttatcgaagc tcagttcgcc gctcagtttc aggtgcgtca acaagggatc
     2101 gacctgttcg ccgaggccgc tcaggctgtc gagcttcgcc ttgacgggga tgatgatggc
     2161 ggcgccgtcc ttgtggctgc ggccctgcgc gtcgaggccg ctcaggcgga tgtcgccgta
     2221 gccgaagcgt ttggcggcga tgtcgtaatc gatgcgcggc gtctcgaagg cgccgtcgaa
     2281 caccagatcg gcgcgcaggc cgtcggcgct gacgtccttg ccgagcgcgt cgggccgcag
     2341 caggcgcgcg tgcgcctcca gctttcggaa gcggttgtgg ccgagatcga ccacgcccag
     2401 cgccttgacg tcgacggcct gcgaggccag cgccaggttc aggtcgaaca cgcggtcctt
     2461 catcttcgcc gtggcgtcga tattgagctg cggcctgagc agggcggcgg cgtccgcgcc
     2521 ggcgatcagg tccgggtgcg tcggtccggc gatatgaaac acgccgttct gcgccgtcag
     2581 ttgcaggtcg ctcagcgccg cgccgccgaa ttcggtgacg aggcggcccc gccacagcgc
     2641 ccagccgccc ttgccgtcga gatcgaggct gagcggcttg ccgaggccca gcagcctgtc
     2701 cgccgcgccg tcctccggcg ccagggcgtg cgcttcgagg tcgagtttat tggcgtccgg
     2761 cacggcgtcg aggcgcaggg tcagccggtc gcccttgtcg ctgagcgcct ccagcgccgc
     2821 ctgcgcgcgc ccgcgcagga tatgcgccgc gccgccgaag cgcagcgtgt agggctggcc
     2881 cgccaccggc gcgtcgagga tgagcgcgcc gacctgcacg cggttcaggt cgatgcgcag
     2941 gtcgggcagg atcgggccgg atttttccgg ctcctgcgac gggttgagga cgggctggcg
     3001 caacacctcg atgcgcggcg cgtcgaggtc gcggatgtcg acatgtttgt tcagatagcc
     3061 gaacgggcgc cagtcaagat tgacgacggg cgcctcgatg aacacgccct tcgtgtccac
     3121 cacgcgcagg ccgtggatgg tcatcttgcc gtacagcgag ccgtcgatgc ggtcgatctg
     3181 gaattgcagg ccggaattga gcttgatccc ggtggcgaac tgcaccagca ggcggcggcc
     3241 cgccggggtg ttgagcgcgg ccagaaacgc ggtcagcagc agcagaatcg ccgccgcgat
     3301 cgccgccgtg cgccggaaga cgcggcccca gcggatgtcg ctcaggcgga acccggcctt
     3361 gcgcgccagg gtttcggcgg ccgccttgat ctgttcgaca ggcgtttcct caggcggcgt
     3421 ttcgggagac gtttcgggcg cgtcgtccag ccggagcggt tttttcgggg gtttcctcgc
     3481 catctgcgcc cccctagaag ctctggccga tgccgacata gagcgccacg atcggatcgc
     3541 ccttgtggcg gttgatcggg gtggcgacgt cgatgcggat cgggccgaaa ttggtgtaga
     3601 ggcgcgcgcc gatcccggcg ccgaagcgca ggtccgagaa ggacggcgtg gtcgaggcgt
     3661 aggcctgtcc ggcgtcgatg aacggcacga cgccgatgtc gccgatgcgg tagcgcgcct
     3721 cgaggctggc ctcgacgacg ctgaggccgc cggtggggtt gcccttggcg tcgacggggc
     3781 cgagcgcctg atagccgtag ccgcgcacgc tgccgccgcc gcccgcgtac agccgccgcg
     3841 acggcgcgat gctggcgagg ccgccgccga tcagcgtgcc gatgcgcccg cgcatggcga
     3901 tgaccagctt ggcgccgacg gggtgataat agctggcgtc gaagatttcg ctgaggttcg
     3961 agccgcccgc cgacagcgag atctgcggcg tggactggct ggtcaagcga aagcccgtgg
     4021 tcgggttcag caggtcgttg gagcggtcgt agccgatctt caccggcaga tcgaggtagt
     4081 aatagttctg ccagaccgtg ccggcgttat ggttcaggtc gttggattcg cggctggctt
     4141 cgaggttgaa gccgtaggac cacgtccaca ccttctgcca cagcggcgtc gagacgcgcg
     4201 agatcgagcc ggacaggccc gccgcatagg cttcgtagga atcgtagata ttgtgactga
     4261 ggctggcgct taactgcacg gtgcggtcgc gcttttgggc gttggagcgg cgcagggtgg
     4321 tcgtcaggct ctgctgctgc gtgccgagcg cgccggtcag gatcagcgcg ccttcgggcg
     4381 ggaacttgtt gcggttctgc caactgccgg tggcggtgaa gccttcgccg gtcgaatagc
     4441 cgatctcgcc cgccagcgta tgcaccggcc cggcctgctg atgcaccaga aggtcgacat
     4501 aggacgtgcc gtcgggcgcc ttttcgcccg tggcgaccgg ctcgaccgag acgctgcgga
     4561 acaggccggt ggcgaccagc gcctcgcgca ggtcgttgac cttgcggttg tcgtagaggt
     4621 cgccgcgcga gaagcgcgtc agctcgccga catgcttcgg gctgaaggcc agcttgccgt
     4681 cgctgcggaa gccgccgaag cgcgagcggt cgccggtgtc gacgtgcagc gtatagtcgc
     4741 cggtggcgtc ggccgggtcc agcgcgatgt cgcgcgcgcc gattttggcg aacggatagc
     4801 cgttttcctg catgccgacg ctgacgctgg cctcggcggc cagcacggcg tcggcaagta
     4861 tagggtcgcc ctgcgtcagc ggcagcgcgt tgcggatcag gtcgggcggc tgggtcggat
     4921 tggcgtcgat cctgatcagg tcgaagcggt agagcgggcc gggaaccgcg accagactgg
     4981 ccgtcaccgg gccgttcgcg tcgggcgccg gtttcagcgt ggtggtcacc gcgccgtcga
     5041 aatagccgcg cgagttcagc aggcgcagga tcagcgcctc gtcctcgctg gcgcgggcgc
     5101 gcaccatggc gacgttggcg gcgcggccct tgccgttttc gagcgcgctc agggccttga
     5161 actcggcggc gactcccacc gccttcaggc ccgccacctg cgtgacgtag cggatttccg
     5221 gcgcggcctc ggcgggcgcg gcggcctgtt gcggctgtac gtcatagcct tgcaacggcg
     5281 gcagcggcgc ggtcaggtcg ggatcgtcgc tggcgccgac gggcggcagg gcgtccggtt
     5341 cggcgggctg ctccggcgtg ggcggcgcct gagtctgagg cgcctgagtc tgagaagcct
     5401 gagtctgggg agcctgagtc ttgacctgag tcgggggggc ggcgtcgagc gggggcaggg
     5461 cggcgtcgaa cgcctgatcg gtgatgatgg gcggattggc ctgcgcgggc gcggcggctt
     5521 gcgcgaaggc ggcgccgtgc gccagcaacg ccatcgccag ccctgtcgcc gcgcctgtca
     5581 tcgggcgtgc gccatgcagg gcggcgaaga tggggcggcg gtgcgaaata gggaacgctc
     5641 ggtattagtg acgaaacaag gcttctatag tcatgcgtgt attaaatttg cacaaaaaat
     5701 cgatacggaa cggcgcgcga tgcgggcgaa tttgaccgca agcggcggag tgcggcgccg
     5761 gcgccgcagg gctagtctgg cgtcgtcccg atcgcgggac tatatctgga gcccgccatg
     5821 acccatgctg ttccctctgc tgtcgaagcc gacccgcgct ggcgcgcggt gctgcggcgc
     5881 gacgccgccg atttcgtcta tggcgtcatg acgaccggcg tctattgccg tccgtcctgc
     5941 ccgtcgcggc ggccgaagcc ggaaaacgtc gcctttttcg acagttgcga cgcggcggag
     6001 gcggcgggat tgcgggcctg cctgcgctgc cgtccgcgcg gcgaagaccc ggaggcgcg
//
LOCUS       DASVLL010000013         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_323277,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000013 DASVLL010000000
VERSION     DASVLL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323277"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF402_00225"
     CDS             complement(<1..186)
                     /locus_tag="VF402_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798741.1"
                     /translation="MLIDASKPARAAPGAHRYNLVYIWAISATAALGGLLFGYDWVVI
                     GGAKPFYEPYFRLTSDAQ"
     gene            598..969
                     /gene="rpsL"
                     /locus_tag="VF402_00230"
     CDS             598..969
                     /gene="rpsL"
                     /locus_tag="VF402_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX7798742.1"
                     /translation="MPTVNQLIRKPRHPKPVRNKVPALKGCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTSQIEAVCYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHILRGVL
                     DTQGVKNRKQRRSHYGAKRPK"
     gene            985..1458
                     /gene="rpsG"
                     /locus_tag="VF402_00235"
     CDS             985..1458
                     /gene="rpsG"
                     /locus_tag="VF402_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX7798743.1"
                     /translation="MSRRHRAEKREVLPDPKFGDLVVTKFMNYVMYEGKKAVAENIIY
                     GAFDILEAKKKDATALETFHSALDNVSPSIEVRSRRVGGATYQVPVEVRPDRRRALAI
                     RWLVNAARKRGENTMTEKLAAELLDAANNRGTAVKKREDTHKMAEANRAFSHYRW"
BASE COUNT          381 a          568 c          500 g          302 t
ORIGIN      
        1 ctgggcgtcc gaggtcaggc ggaaatacgg ctcgtagaac ggcttggcgc cgccgatcac
       61 cacccagtcg tagccgaaca gcagcccgcc cagcgccgcc gtcgcggaga tcgcccatat
      121 atagacgaga ttataacgat gcgcgccggg cgcggcgcgg gcgggcttgg aggcgtcgat
      181 cagcatggcg gtccctgtcg gttatgccga tcagttaaat tcgatcataa gtgaaagtca
      241 ataaatcata aaatatgatc attattgacc gaagcggcgc ggcgcgcgcg gcgttaagaa
      301 ttccggcccg ccgcccttga ccccgccagc cccttcctat ataggccgct gatcattttc
      361 gaggcggtcc cttgcgggac ctttcgggcc ggacttttac aggggctgcg tccccgccgt
      421 aacccccaga gcgcgggttg cggcttttgt gtttgtccgt ctggtctcta aaatagcctc
      481 atgccggaaa gtggtcgcca ctttttggaa aaacatgagg cgtcagataa agacacatcc
      541 caaccgaagc cctccggcca aacggcacag gcttccccta cgaagagttg agcatctatg
      601 cccacagtca accagctgat ccgcaagccg cgtcacccca agccggtccg caacaaggtt
      661 ccggccctga agggctgccc gcagcgccgc ggcgtctgca cccgcgtcta caccaccacg
      721 ccgaagaagc cgaactcggc gcttcgtaag gtcgccaagg tccgcctgac ctcgcagatc
      781 gaagccgtgt gctacatccc cggcgaaggc cataacctgc aggagcactc ggtggtgctg
      841 atccgcggcg gccgcgtcaa ggatcttccg ggcgtccgct atcacatcct gcgcggcgtg
      901 ctcgacacgc aaggcgtcaa gaaccgcaag cagcgccgtt cgcattacgg cgcgaagcgt
      961 ccgaagtaat aggagcactt cactatgtcc cgtcgtcacc gcgcagagaa gcgtgaagtt
     1021 ctgcccgacc ccaagttcgg cgatctggtc gtcaccaagt tcatgaacta cgtcatgtac
     1081 gaaggtaaga aggccgtcgc tgaaaacatc atctatggcg ccttcgacat tctcgaagcc
     1141 aagaagaagg atgcgaccgc gctcgaaacc ttccactcgg cgctcgacaa cgtctcgccc
     1201 agcatcgaag tccgttcgcg ccgcgtcggc ggcgccacct atcaggtgcc ggtcgaggtc
     1261 cgcccggacc gccgccgcgc cctggccatc cgctggctgg tcaacgccgc gcgcaagcgt
     1321 ggcgagaaca ccatgaccga aaagctggcc gctgagctgc tggacgccgc caacaaccgc
     1381 ggcaccgcgg tcaagaagcg cgaagacacg cacaagatgg ccgaagccaa ccgcgccttc
     1441 tcgcactatc gctggtaagc gacggcgaaa gcgggcggcg ggtctttcgg cctgtcgcaa
     1501 aaatatggct ttcaggcgcg ggcggtttgg tttaaaccgc ccgcgcttat tacacccggc
     1561 gatcgccaaa aagtgggacg cacttcttgg aagcggatcg cggtaatacg aaatgaaatc
     1621 tgattgaatt ccgaccgaaa gttgaggcta tcccatgccc cgcagtcaca agattgaaga
     1681 ctatcgcaac ttcggcatca tggcccacat cgatgccggc aagaccacca cgaccgagcg
     1741 catcctgtac t
//
LOCUS       DASVLL010000014         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_323279,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000014 DASVLL010000000
VERSION     DASVLL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323279"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1282)
                     /locus_tag="VF402_00240"
     CDS             complement(77..1282)
                     /locus_tag="VF402_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7798744.1"
                     /translation="MLKRALLTSLGLLLLASPAFAGDAGWSATWASSQMTPTGDNALP
                     AGDTSDVTIRQIVHVSGGGAKIRIRVSNAMSDAPLRIKDVHVALGKLGSDAVDADSDR
                     AVTFGGEDEVLVPSFGEYVSDPISLNVPDGASLAVTFYIPGPISQQTGHPGSRATTYI
                     VHGDHTGAPALPGAEKRANWFNLGAVDVQNDGATIVALGDSITDGHAVDTDSNGRWTD
                     ALAARLADRDISVVNEGIGGNRLHLDGLGPNAVARFDRDALGISGAKWVIVFEGVNDL
                     GTATRDAPIPPEAHDALVNQIISDYQQLIYRAHARGLKIFGATITPFAGNDYYHPGPE
                     TETDRQKINAWIRTSGAFDGVIDFDALIRDPKAPDHILPAYDSGDHLHPGMAGYKAMG
                     EAVNLDLFK"
     gene            1407..>1672
                     /locus_tag="VF402_00245"
     CDS             1407..>1672
                     /locus_tag="VF402_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798745.1"
                     /translation="MLTQERLNAMALELPGTEPGTSYGYPAVKLGKKTLVVWSPKWNA
                     PVFGMDFETRDFYIEADPETFFTTDHHRNWPCVLARPETVDEAWV"
BASE COUNT          266 a          513 c          606 g          287 t
ORIGIN      
        1 ccgacggaac cggcgcaagc tccgttttcg ccgccctgaa gcgcgtaagg ccgcagcgcg
       61 taaggcggcc ttcgcgctat ttgaacaggt ccagattcac cgcttcgccc atcgccttgt
      121 accccgccat gccgggatgc aggtgatcgc cggaatcgta ggcgggcagg atatggtcgg
      181 gggccttcgg atcgcggatc agggcgtcga agtcgatcac cccgtcgaag gcgccggacg
      241 tcctgatcca ggcgttgatc ttctggcggt ccgtctcggt ttccgggccg gggtgataat
      301 agtcgttgcc ggcgaagggc gttatggtgg cgccgaaaat cttcagcccg cgcgcatggg
      361 cgcggtagat cagttgctga taatcgctga tgatctggtt caccagcgca tcgtgcgctt
      421 cgggcgggat cggcgcgtcg cgcgtcgccg tgccaagatc gttgacgccc tcgaagacga
      481 tgacccattt ggcgccgctg atccccagcg cgtcgcggtc gaagcgcgcc accgcgttgg
      541 ggccgaggcc gtcgagatgc agccggttgc cgccgatgcc ctcattgacc accgagatgt
      601 cgcggtccgc cagacgggcg gccagcgcgt cggtccagcg cccgttgctg tcggtgtcga
      661 ccgcatggcc gtcggtgatc gagtcgccca gcgccacgat cgtcgcgccg tcgttctgaa
      721 cgtcgacggc gcccagattg aaccagttgg cgcgcttttc cgcgccgggc agggcggggg
      781 cgccggtgtg gtcgccgtgg acgatatagg tggtggcgcg cgatcccgga tggccggtct
      841 gctggctgat cgggccggga atgtagaagg tgacggccag gctggcgccg tcggggacgt
      901 tgaggctgat cgggtcggag acatattcgc cgaaggacgg gaccagaacc tcgtcctcgc
      961 cgccgaaggt cacggcgcgg tcgctgtcgg cgtcgacggc gtccgagccc agcttgccca
     1021 gcgcgacatg cacgtccttg atgcgcagcg gcgcgtcgct catggcgttg gagacgcgga
     1081 tgcggatttt cgcgccgccg ccggacacat gcacgatctg gcggatcgtg acgtcggacg
     1141 tatcgccggc gggcagggcg ttgtcgccgg tcggcgtcat ctgcgacgac gcccatgtcg
     1201 cgctccagcc cgcgtcgccg gcgaaggcgg gcgaggcgag cagcagcagg ccgaggctgg
     1261 tgagcagagc gcgtttcagc atgatcaaat atccctgtcc ggtcattgtt agcgctatcc
     1321 gtaagacggg ccgcgggtgc tgacaagcgg caaaacggtt gacgcggcct gagcgcggat
     1381 taggctgcga tccggcggag gacgatatgc tgacacagga acgcctgaac gcgatggcgc
     1441 tggaactgcc ggggacggag ccgggaacct cgtacggtta tccggcggtc aagctgggca
     1501 agaagacgct cgtcgtgtgg agcccgaaat ggaacgcgcc ggtgttcggc atggatttcg
     1561 agacgcgcga cttttacatc gaggccgacc cggagacctt cttcaccacg gaccatcacc
     1621 gcaactggcc gtgcgtgctg gcgcggccgg aaacggtgga cgaggcctgg gt
//
LOCUS       DASVLL010000015         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_269765,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000015 DASVLL010000000
VERSION     DASVLL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269765"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..506)
                     /gene="ndk"
                     /locus_tag="VF402_00250"
     CDS             complement(90..506)
                     /gene="ndk"
                     /locus_tag="VF402_00250"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7798746.1"
                     /translation="MSRTFSIIKPDATRRNLTGAINAVIEAAGLRIVAQRRVHMTRAQ
                     AERFYGVHKERPFFGELVEQMTAAPVVVQVLEADNAVPTYREVMGATNPEQAADGTIR
                     KQFALSIGENSVHGSDSDENAAIEIAQFFSEADIVG"
     gene            601..2502
                     /locus_tag="VF402_00255"
     CDS             601..2502
                     /locus_tag="VF402_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7798747.1"
                     /translation="MLQITGLVFDAYGRRFFDGAGLTLAPGVKAGLVGLNGVGKSTLF
                     KLILGQHQPGGGEILMPKAWRVASVDQEIAASPQHLIDAVLALDVRRANLLKALETAD
                     PMHQAEIHHDLYAIQADRAPSRAAEILSGLGFSNADLTRPISDFSGGWRMRAALAGAL
                     LAEPDLLLLDEPTNYLDLEGALWLEARLKRYPNAALMISHDRDLLNEAVDAIVHVVGQ
                     KLDYYSGNYDNFEQMRAEKARLNAANRVKQEAERAHLQSFIDRFGAKASKATQAQSRM
                     KKLAKLPPIAANIEDRVAPFILPSPEKQLAPPVLRLEDVSIGYDDAVILRNVNIRLDP
                     DDRIGVLGVNGAGKSTFAKLLAGALTEKHGVQWRDRRMSVAWFHQHQIEAMDPEDTPL
                     EMMRRVRPEESESKRRARLGSFGMTVEKVETKVKDLSGGERARLLLNMVAMDGPHLLI
                     LDEPTNHLDIDSRRALLDALNDYEGAVLIITHDRSLIELVADRLWLVDQGTVKTFTGS
                     MDDYARLVIERAKTATREEAAAGAAPKSANVNTKEARKAAAAARQAIAPLKKKADDLE
                     RKIETTGNQIKLIDHRLADPDLYVKDKEKAVSFGKEKAKLESDLVRLEAEWMDAAEAY
                     ETARVEAGV"
BASE COUNT          501 a          854 c          776 g          417 t
ORIGIN      
        1 ttacctataa gaaaagcccg gccttttagg ccgggctttt cttatagggt agcgaagatg
       61 tgggggcaat cgcccccacg ccccgttact tagccgacga tatccgcctc cgagaagaac
      121 tgcgcgatct cgatcgccgc gttctcatcc gaatccgaac cgtggaccga gttttcaccg
      181 atcgacaaag cgaactgctt gcggatcgtg ccgtcggcgg cctgttccgg gttggtggcg
      241 cccatgactt cgcggtaggt cggcacggcg ttgtcggctt ccagaacctg caccacgacc
      301 ggcgcggcgg tcatctgctc gaccagctcg ccgaagaacg gacgttcctt gtgcacgcca
      361 tagaaacgct cagcctgagc gcgggtcata tggacgcggc gctgcgccac gatgcgcaag
      421 cccgcggctt cgatcacggc gttgatcgcg ccggtcaggt tgcggcgggt ggcgtccggc
      481 ttgatgatgg agaaggtacg ggacatagga gttccatttt tgcttggtat ttaagattgg
      541 cgcgcttata gcgacctcca ccccgttcgc caaccctgaa ttttccgaag ttttcgtgtc
      601 atgctgcaaa tcaccggtct ggttttcgac gcctacggcc gccgtttctt cgacggcgcc
      661 ggcctgaccc tggcgccggg cgtcaaggcg ggcctcgtcg gcctcaacgg cgtcggcaaa
      721 tcgaccctgt tcaagctgat cctcggccag caccagcccg gcggcgggga aatcctcatg
      781 ccgaaagcct ggcgcgtcgc ctcggtcgat caggaaatcg ccgcctcgcc gcagcacctg
      841 atcgacgccg tgctggcgct cgacgtgcgc cgcgccaacc tgctcaaggc gctggaaact
      901 gccgatccga tgcatcaggc ggaaatccac cacgatctct acgccatcca ggccgaccgc
      961 gccccgtcgc gcgcggccga aatcctgtcc ggcctcggct tctccaacgc cgacctgacg
     1021 cggccgatct cggacttttc cggcggctgg cggatgcgcg cggcgctggc cggagcgctg
     1081 ctggccgaac ccgacctgct gctgctcgac gaaccgacca actatctcga tctcgaaggc
     1141 gcgctgtggc tggaagcccg gctcaagcgc taccccaacg ccgcgctgat gatctcgcac
     1201 gaccgcgacc tgctcaacga agcggtcgac gccatcgtgc atgtggtcgg ccagaagctc
     1261 gactattaca gcggcaatta cgacaatttc gagcagatgc gcgccgaaaa ggcccgcctc
     1321 aacgccgcca accgcgtcaa gcaggaggcc gaacgcgccc acctgcaaag cttcatcgac
     1381 cgcttcggcg ccaaggccag caaggcgacg caggcccagt cgcgcatgaa gaagctggcc
     1441 aagctgccgc ccatcgccgc caatatcgag gaccgcgtcg cgcccttcat cctgccctcg
     1501 ccggaaaagc aattggcgcc gccggtgctg cggctggagg atgtcagcat cggttatgac
     1561 gacgccgtca tcctgcgcaa cgtcaacatc cggctcgatc ccgatgatcg tattggggtt
     1621 ttgggcgtca acggcgccgg taaatcgacc ttcgccaagc tgctggccgg cgccctgacc
     1681 gaaaagcacg gcgtgcaatg gcgcgaccgg cgcatgagcg tcgcctggtt ccaccagcac
     1741 cagatcgagg cgatggaccc cgaggacacg ccgctggaga tgatgcgccg cgtccgcccg
     1801 gaggagagcg aatcgaagcg ccgcgcccgg ctcggctcgt tcggcatgac ggtcgaaaag
     1861 gtcgaaacca aggtcaagga tctgtccggc ggcgaacgcg cgcgcctctt gctcaacatg
     1921 gtggcgatgg acgggccgca ccttttgatc ctcgacgagc cgaccaacca cctcgacatc
     1981 gattcgcgcc gcgccctgct cgacgcgctc aacgattacg aaggcgcggt gctgatcatc
     2041 acccacgacc gctccctgat cgaactggtc gccgaccgcc tgtggctggt cgatcagggc
     2101 acggtcaaga ccttcaccgg cagcatggac gactacgcca gactggtcat cgaacgcgcc
     2161 aagaccgcca cgcgcgagga agccgccgcg ggcgccgcgc cgaaatcggc caacgtcaac
     2221 accaaggagg cgcgcaaggc cgccgccgcc gcgcgtcagg ccatcgcgcc gctgaagaaa
     2281 aaggccgacg atctggagcg caagatcgag acgaccggca accagatcaa gctgatcgac
     2341 catcgcctgg ccgatcccga cctttatgtc aaggataagg aaaaggccgt aagcttcgga
     2401 aaggaaaagg caaaactgga atccgatctc gtccggctcg aagccgaatg gatggacgcc
     2461 gccgaagcct acgaaaccgc gcgcgtcgag gcgggggttt aaagcgtttc cccaaaaagt
     2521 ggttccactt tttggataag gaagagcg
//
LOCUS       DASVLL010000016         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_108403,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000016 DASVLL010000000
VERSION     DASVLL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108403"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1078)
                     /locus_tag="VF402_00260"
     CDS             complement(89..1078)
                     /locus_tag="VF402_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7798748.1"
                     /translation="MASIQDVAKLAGVSTATVSRVLSGYAHVKPDMKARVTQAVDSLG
                     YAPNALARNLRTLKTSRLIVSVPDITNIFFSSIIRGAEEAASAAGYAVLLSDAGSGPE
                     TGDAYAGLLRRREADGLICLGHRLPDALGALADRPGAPIVHGCEFDAAHPVSGVQIDN
                     AGAAREVMAYLHGLGHRRIAVLGGQDDSQVSRARFRGVAGARDGLDALTCLNGDFSID
                     SGAALAAELLSRTPRPTAIFCFSDDMAMGALHAVRQAGLSCPRDVSVVGFDDIAAARF
                     TAPPLTTVRQPMREIGRRCVRVLLAILNGERTERVIETLPHELVTRASTAAPE"
     gene            1164..>2262
                     /locus_tag="VF402_00265"
     CDS             1164..>2262
                     /locus_tag="VF402_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7798749.1"
                     /translation="MRLGMTMMMGAALAGWTGAAAARTTEVVVRANRFAAYSGDAAFS
                     TVDAGADDLRGAPDLDTALKRAAQAGLFRRSSSSTANPTVQGVGLRALAPSGAGRALV
                     TLDGVPQNDPFGNWVIWPAIPQMALSHAHVVRGAGGGAYGAGALTGVIDLDLTPPQAL
                     TPYVRVEGGEHGNGRLDAGGALGDLAVYGMTQSLHGDAPVRGAARGAADVATFGRDAA
                     LNADYQRDLCPLGVCGRLDLLAGGYDSRRDTGLRGATATARGDSYSLSFTRSPAPDVN
                     GFRAQVWRKTSDLSNVSVSIAGDRSATALANAQVKTPATGAGFNIAVRHQTPALEWEV
                     GADGRFYDGESREFYRYMSGAATRYRTAGGRS"
BASE COUNT          309 a          795 c          854 g          304 t
ORIGIN      
        1 ggccggactc gaagcgccgc gccagcacgg ccgccgtcag ccagcccgcc gttccgccgc
       61 ccaggatcag gagggtgcgc atgccgcctt actccggcgc cgccgtgctg gcgcgcgtca
      121 ccagttcgtg cggcagggtc tcgatcacgc gctccgtccg ctcgccgttg aggatggcca
      181 gcagaacccg cacgcagcgg cggccgatct cgcgcatcgg ctggcgcacc gtggtcagcg
      241 gcggggcggt gaaacgcgcg gcggcgatgt cgtcgaagcc gaccaccgag acgtcgcgcg
      301 ggcaggacag gcccgcctgc cgcacggcgt gcagcgcgcc catcgccatg tcgtcgctga
      361 agcagaagat cgccgtcggg cgcggcgtgc gcgaaagcag ttccgccgcc agcgccgcgc
      421 cgctgtcgat gctgaaatcg ccgttcaggc aggtcagcgc gtccagaccg tcccgcgccc
      481 cggcgacgcc gcggaagcgg gcgcggctga cctggctgtc gtcctggccg cccagaacgg
      541 cgatgcgccg gtggccgagg ccgtgcagat aggccatgac ctcgcgcgcg gcgcccgcat
      601 tgtcgatctg cacgcccgac accggatgcg ccgcgtcaaa ttcgcagccg tggacgatcg
      661 gcgcgccggg ccggtccgcc agcgcgccga gcgcgtcggg caggcggtgg ccgaggcaga
      721 tcaggccgtc ggcctcgcgc cgccgcaaaa ggcccgcata ggcgtcgccc gtttccggcc
      781 cggagcctgc gtcgctcagc agcaccgcat agcccgccgc cgaggccgcc tcctcggcgc
      841 cgcggatgat gctggaaaag aagatgttgg tgatgtccgg cacggagacg atcaggcgcg
      901 aggtcttcag ggtgcgcagg ttgcgcgcca gcgcgttggg ggcgtagccg aggctgtcca
      961 ccgcctgcgt cacgcgcgcc ttcatgtccg gcttgacgtg ggcgtagccc gacagcacgc
     1021 gcgatacggt ggccgtcgac acgcccgcca gtttcgccac gtcctgaatg ctcgccaagg
     1081 ccgaagtcct ttttcaaatc gccgtattga ctgtttacgg cgatccggtt tgcgctgcca
     1141 ccggaaaatc cgggaatggc gcgatgcggc tgggcatgac gatgatgatg ggggcggcgc
     1201 tggcgggctg gacgggcgcg gcggcggcgc ggaccaccga ggtggtggtg cgcgccaacc
     1261 gcttcgcggc ctatagcggc gacgcggcct tcagcacggt cgacgccggg gcggacgatc
     1321 tgcgcggcgc gcccgatctc gacacggccc tgaaacgcgc ggcgcaggcc ggtctgttcc
     1381 ggcgctcgtc gtccagcacc gccaatccga ccgtgcaggg cgtcggcctg cgcgcgctgg
     1441 cgccgtcggg ggccgggcgg gcgctggtca cgctggacgg ggttccgcag aacgatccgt
     1501 tcggcaactg ggtgatctgg cccgctattc cgcagatggc gctcagccac gcccacgtgg
     1561 tgcgcggcgc gggcggcggg gcctatggcg cgggcgcgct gaccggggtg atcgacctcg
     1621 acctgacgcc gccgcaggcg ctgacgccct atgtgcgcgt cgagggcggc gaacacggga
     1681 acgggaggct ggacgcgggc ggcgcgctcg gcgatctggc cgtgtacggc atgacgcaga
     1741 gcctgcacgg cgacgcgccg gtgcgcgggg cggcgcgcgg cgcggcggac gtggcgacct
     1801 tcggccgcga tgcggcgctg aacgccgatt atcagcgcga tctttgcccg ctcggcgtct
     1861 gcgggcggct cgacctgctg gcgggcggtt acgacagccg ccgcgacacc ggcctgcgcg
     1921 gcgcgaccgc gacggcgcgt ggcgacagtt acagcctgag cttcacgcgc agcccggcgc
     1981 cggacgtgaa cggctttcgc gcgcaggtgt ggcgcaagac ctcggacctg agcaatgtct
     2041 ccgtctcgat cgccggcgac cgtagcgcga cggcgctggc caacgctcag gtcaagacgc
     2101 ccgcgacggg cgccggtttc aacatcgccg tgcgccatca gacgcccgcg ctggaatggg
     2161 aggtcggcgc ggacgggcgc ttttacgacg gcgagtcgcg cgaattctac cgctacatgt
     2221 cgggcgcggc gacgcgctac cgcacggcgg gcgggcgcag tt
//
LOCUS       DASVLL010000017         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_377024,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000017 DASVLL010000000
VERSION     DASVLL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377024"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VF402_00270"
     CDS             complement(<1..267)
                     /locus_tag="VF402_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX7798750.1"
                     /translation="MDNSPALWDLPALRRDGSKYDRGHCVVLGGPAAHTGAARLAARA
                     ALRIGAGLVSIACDPGSLIVYATALEAVMTRVVRSADAFADLIAD"
     gene            376..1317
                     /locus_tag="VF402_00275"
     CDS             376..1317
                     /locus_tag="VF402_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7798751.1"
                     /translation="MSTVLLCDLSLAPQVVFALSAPGRAIYATARARAADFEAFRDAL
                     RLFLDAQDKPVLDGVAVAARGWEQADGLHPIGIGFPLDRETIRELTGVQQVSFVNNFV
                     ARALAVPHLRGEARQQIHGGDRHAEHALAVLGPHYGLGLAALVADGLGGWTALHGEGG
                     HADMPARDEHEWRILEAIRRKDGYVMRESCISMAGLANIHHALHTLSGVHPPDLSAPE
                     IITLARGGDVAARQAIATMTLWLADLASDVTLILGAGGVYLSGAMLDLMDDLFDADLF
                     VSRFLDKGPRSAYVRNVPVFRTRAADLELAGLATLFD"
     gene            complement(1322..1738)
                     /locus_tag="VF402_00280"
     CDS             complement(1322..1738)
                     /locus_tag="VF402_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798752.1"
                     /translation="MSKPHERRLSEAEIAALPACLPARAARLVDAEHWVSWLARWIGR
                     GPRIVVRGERVFWPGAPDELSRDAARLALLAHELTHVWQYANGMTLLRYALCERGVYR
                     YRLDGRAFAAYGYEQQAAMIEDWTRLAHGLPPRWND"
     gene            1737..>2521
                     /locus_tag="VF402_00285"
     CDS             1737..>2521
                     /locus_tag="VF402_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX7798753.1"
                     /translation="MDLPIGLNAETVHMALRIEAPVAGRPGADYNRALTGEDDMAAKP
                     FYAHLYVQVLIGVALGAAFGLIWPHLAAQPWVRLIGDLFVKLIKMTIAVLIFCTVSAG
                     IGKVRSAGQVGRVGVKALVYFEVMSTLALVLGLVVANLTHPGEGLHAAGDAAAVAQFT
                     APDKQETLSGFLSALIPDSFLSGLVKGDILQTLLIAIFTGFALMRLGERAAKVRDLIE
                     EGSRVIFDIVNMIMRLAPLGAGAAMAFTLGEYGATALGGLLELV"
BASE COUNT          366 a          855 c          903 g          397 t
ORIGIN      
        1 gtccgcgatc aggtcggcga aggcgtcggc ggaccggacc accctggtca tcaccgcctc
       61 caacgccgtg gcgtacacga tcaggctgcc cggatcgcag gcgatggaga ccagccccgc
      121 gccgatgcgc aaggccgcgc gcgcggccag ccgcgccgcg ccggtgtgcg ccgccggtcc
      181 gcccagcacg acgcagtggc cgcgatcgta cttgctgccg tcgcggcgca gggcgggcag
      241 atcccaaagg gcgggcgagt tatccatagg cgtttgcggc tccctcaagg cggtttgcga
      301 attattatga caatgggcgc accgtggacg caagcggcca aaaatgaaag acgccgcgat
      361 tgcgggagta cgcttatgag tacggttcta ttgtgcgacc tgtcgctggc gccgcaggtc
      421 gtgttcgccc tgtccgcgcc gggacgggcg atttacgcca cggcgcgcgc ccgcgcggcg
      481 gacttcgagg cgttccggga cgcgctgcgg ctgtttctcg acgcgcagga caagcccgtc
      541 ctggacggcg tcgccgtggc cgcgcgcggc tgggagcagg cggacgggct gcatccgatc
      601 ggcatcggct ttccgctcga ccgcgagacg atccgcgaac tgaccggcgt gcagcaggtc
      661 agcttcgtca acaatttcgt cgcgcgcgcc ctggccgtgc cgcatctgcg cggcgaggcg
      721 cggcagcaga ttcacggcgg cgaccgccac gccgaacacg ctttggccgt gctggggccg
      781 cactatggcc tcggtctggc ggcgctggtc gcggacgggc taggcggctg gaccgcgctg
      841 cacggcgaag gcggccatgc cgacatgccg gcgcgcgacg agcatgagtg gcgcatcctc
      901 gaagccatcc ggcgcaagga cggctacgtc atgcgcgaat cgtgcatttc gatggccggg
      961 ctggccaata tccatcacgc cctgcacacg ctgagcggcg tgcatccgcc cgatctcagc
     1021 gcgccggaga tcatcacgct ggcgcgcggc ggcgacgtcg cggcgcgcca ggccatcgcg
     1081 acgatgacgc tgtggctggc cgatctggcg tccgacgtga cgctgatcct cggcgcgggc
     1141 ggggtctatc tgagcggcgc catgctggac ctgatggacg acctgttcga cgccgacctg
     1201 ttcgtgagcc gcttcctcga caaggggccg cgcagcgcct atgtgcgcaa cgtgccggtg
     1261 ttccgcaccc gcgccgccga tctggagctg gccgggctgg cgaccctgtt cgactaaaac
     1321 gctaatcgtt ccagcgcggc ggcaggccgt gcgccagccg cgtccagtcc tcgatcatgg
     1381 ccgcctgctg ctcgtagccg taggcggcga aggcgcgccc gtccagccgg tagcgataga
     1441 cgccgcgctc gcacagcgca tagcgcagca gcgtcatgcc gttggcgtac tgccacacgt
     1501 gcgtcagttc atgcgccagc agggccagac gcgccgcgtc acggctcagc tcgtcgggcg
     1561 cgccgggcca gaagacgcgc tcgccgcgca cgacgatccg cggcccgcgc ccgatccagc
     1621 gcgccagcca tgagacccaa tgctcggcgt cgaccagccg cgccgcccgc gcgggcagac
     1681 aggcgggcag ggcggcgatc tcggcttcgc tcaggcggcg ctcatgcggc ttcgacatgg
     1741 accttcctat cggcctgaac gccgaaactg tccatatggc tttgcggatc gaagcgccgg
     1801 ttgccggcag gccgggcgcg gattacaaca gggcgttgac cggcgaggac gatatggcgg
     1861 cgaagccctt ttacgcgcat ctttacgtgc aggtgctgat cggcgtggcg ctgggcgccg
     1921 ccttcgggct gatctggccg catctggcgg cgcagccttg ggtgcggctg atcggcgacc
     1981 tgttcgtcaa gctgatcaag atgaccatcg ccgtgctgat cttctgcacg gtcagcgccg
     2041 ggatcggcaa ggtgcgctcc gccggtcagg tcgggcgcgt cggcgtcaag gcgctggtct
     2101 atttcgaggt catgtccacc ctggctctgg tcctcggcct ggtcgtcgcc aacctgaccc
     2161 atccgggcga ggggctgcac gcggcgggcg acgcggcggc ggtggcgcag ttcaccgcgc
     2221 cggacaagca ggagacgctg agcggcttcc tctcggccct gatcccggac agcttcctga
     2281 gcggcctggt caagggcgac atcctgcaaa ccctgctgat cgccatcttc accggtttcg
     2341 cgctgatgcg gctgggcgag cgggcggcga aggtccgtga cctgatcgag gagggcagcc
     2401 gggtcatctt cgacatcgtc aacatgatca tgcggctggc cccgctgggg gcgggcgcgg
     2461 cgatggcgtt cacgctgggc gaatacggcg cgacggcgct gggcgggctt ctggagctgg
     2521 t
//
LOCUS       DASVLL010000018         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_162188,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000018 DASVLL010000000
VERSION     DASVLL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162188"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VF402_00290"
     CDS             <1..888
                     /locus_tag="VF402_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEX7798754.1"
                     /translation="RAKASLMAASAAHMEQAGEDVDQALDYAVRAAKLQPQFTPAPVI
                     AARLLARANKLGRAEDTLEAAWGANPHPAIWLTYRDLVSDETPRERARRLQGLVDRNP
                     QHRESRLLMLEKALLTGIKPDIAAAMDALNEDAAEDRLTRRLGGLMARGAQALGDIDG
                     ARAWLAAATLSKGEPDWSDIDSEGRAFPYGAADWSRVILTFAETAALAHPRFERGEKG
                     LPEMPDVASRYVPSMPFIRAAQRSAHRGDSLLMPDDPGAPDASIIDAAVSGQDLDQDA
                     PVEAPARAPRRKTAPKTRK"
     gene            982..1057
                     /locus_tag="VF402_00295"
     tRNA            982..1057
                     /locus_tag="VF402_00295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1015..1017,aa:Thr,seq:cgt)
     gene            complement(1028..1336)
                     /locus_tag="VF402_00300"
     CDS             complement(1028..1336)
                     /locus_tag="VF402_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798755.1"
                     /translation="MPLAPFDMILVIAAAIALIALAYNSAGIVRFLKRPWGVSGREIE
                     AKASEMLARNPDAAYRAYLRHEQAWYDQDGAAQIYWLRIRFAIWRKRKKWCRLGDSNT
                     "
BASE COUNT          299 a          532 c          476 g          243 t
ORIGIN      
        1 cgcgccaagg cgtcgctgat ggccgcctcc gccgcccata tggagcaggc gggcgaggac
       61 gtcgatcagg cgctggacta cgccgtgcgc gccgccaagc tgcaaccgca gttcacgccc
      121 gcgccggtga tcgccgcgcg gctgctggcg cgcgccaaca agctgggccg cgccgaggac
      181 acgctggagg ccgcctgggg cgcgaacccg catccggcga tctggctgac ctatcgcgac
      241 ctcgtctcgg acgagacgcc gcgcgagcgc gcccgccgcc tgcaagggct ggtcgaccgc
      301 aacccgcagc accgcgaaag ccgcctgctg atgctggaaa aggcgctgct gaccgggatc
      361 aagcccgaca tcgccgccgc gatggacgcg ctgaacgaag acgcggccga ggatcgcctg
      421 acgcggcggc tgggcgggct gatggcgcgc ggcgcgcagg cgctgggcga catcgacggc
      481 gcgcgcgcat ggctggccgc ggcgacgctc agcaagggcg agccggactg gtccgacatc
      541 gattccgagg gccgcgcctt cccctacggc gccgccgact ggagccgcgt gatcctgacc
      601 ttcgccgaaa cggcggcgct ggcgcatccg cgcttcgagc gcggcgaaaa gggcctgccg
      661 gaaatgccgg acgtcgcctc gcgctacgtg ccgtccatgc cgttcatccg cgccgcgcag
      721 cgttcggccc atcgcggcga cagcctgctg atgcccgacg atcccggcgc cccggacgcg
      781 tccatcatcg acgccgccgt ctccggccag gatctcgatc aggacgcccc cgtcgaagcg
      841 cccgcccgcg ccccacgccg caaaaccgcg cccaagaccc gaaaataagg ctgttaatcc
      901 cgcatttcga cccttggcaa gcccgcgcga catgtgtaaa gaaacgcccc tgtccacggg
      961 gccaaccccc ggacgttccg ggccgcttta gctcagccgg tagagcatcg cattcgtaat
     1021 gcgagggtca ggtgttcgag tcacctaagc ggcaccactt tttccttttt ctccagatgg
     1081 cgaaccggat gcgcagccag tagatttgcg ccgcgccatc ctgatcgtac caagcctgtt
     1141 catgtcgcag atatgcacga taggccgcat ccgggttcct cgccaacatc tcagacgcct
     1201 tggcttctat ttcgcgacct gacacgcccc agggccgctt gaggaagcga acgattcccg
     1261 cactgttata ggcgagcgcg atcaaggcga tagccgcagc tatgaccaaa atcatatcga
     1321 agggagctaa aggcatggaa tctgtcgtga tttaagaatt tatacctctg aagttatata
     1381 atcagacgta taaatccgca atcaccagaa ccagatgttc tgattcatag atttcggaag
     1441 ctgaatctca ccttgcttcg gcaaggagac gaagcgtgga acttcagaag cttttcggcg
     1501 ccaatgtgcg caacctgcgc aaagcccgcg gctggacaca ggaacaactc
//
LOCUS       DASVLL010000019         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_215828,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000019 DASVLL010000000
VERSION     DASVLL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215828"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..713)
                     /gene="ssb"
                     /locus_tag="VF402_00305"
     CDS             complement(192..713)
                     /gene="ssb"
                     /locus_tag="VF402_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7798756.1"
                     /translation="MAGSVNKVILVGNLGRDPEIRTLNSGDRVANLTIATSETWRDKM
                     SGERKEKTEWHRVVIFNKNLAEVAEKYLRKGSSVYIEGSLQTRKWTDQNGVEKYSTEV
                     VLQKFRGELTLLGGRGEGGGAGMSGGSSGGYNDYEDSYGGGGGGGAPASSGPRAQPSG
                     PAQNFDLDDEIPF"
     gene            complement(859..1314)
                     /locus_tag="VF402_00310"
     CDS             complement(859..1314)
                     /locus_tag="VF402_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798757.1"
                     /translation="MRIAALSIMALAGCAALAGCTPRVKSPDAPGVCYFIGHPTPKEN
                     KFNVLASNVPDLEHCAVLVYNLRMDMLKTGTAGPETDGAYMGSFLFADNHEVRASQHY
                     EGPVFPFLVKAPDNRLVAPGSIVQQDDAPSGPQTVDIPKNLPQAPSDKK"
     gene            complement(1398..>2039)
                     /locus_tag="VF402_00315"
     CDS             complement(1398..>2039)
                     /locus_tag="VF402_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7798758.1"
                     /translation="RVLGHAEAMEAGALAIADGPAQAMVRGRVRVSTLASLAQLVLLP
                     ALPEVLAAWPELDVDLLAEARNVSLALREADIALRLGRPGDSDLIGRKVGEARFGFFA
                     APGYDAEARLIAYDEDSQAVPEAVWMAQRFPETRRVFRSNSHLIQAEAARRGVGIAHL
                     PLYVARAFGLAPVDFAAPHPPREVWMLTRAAALRTARVRVCCDAFAAALQALP"
BASE COUNT          333 a          722 c          642 g          342 t
ORIGIN      
        1 cacgctcatc gcgtagtcga gatacgaccg tttcagctcg tcttcgatgg cgacgggact
       61 gatccccgcc ggcaaatgcc ctccgtgctg cccctcgccc tgagtctcgt cggtcgtatc
      121 ggtcatttat tcaatatctt agagttaaat cagcccgcgg aaaaaggcgc gtcacggcgc
      181 ggcgaagcgg cctagaacgg gatttcgtcg tcgaggtcga agttctgcgc cgggccggac
      241 ggctgcgcgc gcggaccgga cgaggccgga gcgccgccgc ccccgccgcc gccataggaa
      301 tcctcatagt cgttatagcc gccggacgat ccgccgctca tgccagcgcc gccgccttcg
      361 ccgcgaccgc ccagcagggt cagctcgccg cggaactttt gcagcaccac ctcggtcgaa
      421 tacttctcga cgccgttctg gtcggtccac ttgcgcgttt gcagcgagcc ttcgatatag
      481 acgctggaac ccttgcgcag atacttctcc gccacctcgg ccaggttctt gttgaagatc
      541 accacgcggt gccattcggt cttttccttg cgctcgccgc tcatcttgtc gcgccacgtc
      601 tcggaggtgg cgatggtcag attggccacg cggtcgccgg agttgagggt gcggatttcc
      661 gggtcgcggc ccagattgcc gaccagaata accttgttga cgctgccagc catcttcact
      721 tgcctttatc gttatgcgcg gaacgccgcc cgttatagaa cgactcgatc cggcacacta
      781 gaatcttaac gcgcccaagc caagagccgc cgggcgcgca aggccgcaaa ggggaaggct
      841 catccacaga aggttaattc acttcttgtc cgaaggcgcc tgcggcaggt ttttggggat
      901 gtcgaccgtc tgcggcccgg acggcgcgtc gtcctgctgc acgatcgatc cgggcgcgac
      961 cagccggttg tcgggcgcct tgaccaggaa cgggaagacc ggcccctcgt aatgctggct
     1021 cgcgcgcacc tcgtgattgt cggcgaacag gaagctgccc atataggcgc cgtccgtctc
     1081 cggccccgcc gtgccggttt tcagcatgtc catgcgcagg ttgtaaacca gcaccgcgca
     1141 gtgttcgagg tccggcacgt tgctggccag cacgttgaac ttgttttcct tcggcgtcgg
     1201 atggccgatg aaatagcaca cgcccggcgc gtccggggac ttgacgcgcg gcgtgcaacc
     1261 cgccagcgcc gcgcagcctg ccagagccat gatggatagt gcggcgatac gcatcggaac
     1321 tccccgttcg cggttgtttt tgacgcgccg atttaatcac gatccggggc cgcggaccaa
     1381 gcgaaaaagc cgcccgctca cggcagggcc tgcaaggcgg cggcgaacgc gtcgcagcac
     1441 acgcgcaccc gcgccgtcct gagcgccgcc gcccgcgtca gcatccagac ctcgcgcggc
     1501 ggatgcggcg cggcgaaatc gaccggcgcc agcccgaagg cgcgcgccac atagagcggc
     1561 aggtgcgcga tcccgacgcc tcgccgcgcc gcctcggcct gaatcaggtg gctgttgctg
     1621 cggaagacgc ggcgcgtctc aggaaaacgc tgcgccatcc agaccgcttc ggggacggcc
     1681 tgactgtcct cgtcataggc gatcagccgc gcctccgcat catagccggg cgcggcgaag
     1741 aagccgaagc gcgcctcgcc caccttgcgc ccgatcagat cgctgtcgcc gggccgcccc
     1801 agccgcagcg cgatgtcggc ctcgcgcagc gccagactga cattgcgcgc ctcggccagc
     1861 aggtcgacgt ccagctccgg ccacgccgcc agcacctccg gcagcgcggg cagcagcacc
     1921 agttgcgcca gcgaggccag ggtcgagacg cgcacccgtc cgcgcaccat tgcctgcgcc
     1981 ggtccgtcgg cgatggccag cgccccggcc tccatcgcct cggcgtggcc cagcacgcg
//
LOCUS       DASVLL010000020         7081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_323378,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000020 DASVLL010000000
VERSION     DASVLL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323378"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2911)
                     /locus_tag="VF402_00320"
     CDS             complement(<1..2911)
                     /locus_tag="VF402_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEX7798759.1"
                     /translation="MLGWVLFMLVVLAWRLAAAPIRVDAIRPVIVSRLESALPGTHAS
                     IGHLDLVWFGDARAVGFRFQDLRILDSHHRIVARAGHMEMALAADGLLMARIAPARLT
                     ADDFFLAASVSREGRYDLGYEANGAPSETGGLDKFFYDLTGRERLSRPASFVRQASLR
                     NGEVRLIEQARDPRHMGVDWTAHVARVDFTKLHGRLASHADLSIAPTGAPAATLRAAA
                     EGQVGLKNMVLSASLSHLDPAYVAPSSGVTRLLAGVRAPVSGLARINYTQGRGFENAW
                     LDVSAGAGRLDIGDMHQTFQEARVRVTYDSASHTARFTTFRITSPRIDGDLSGRIVIQ
                     PHNDKTGANMVIAYDFSGPRLRGNLADDYAPQTLTDAHFRGRYVPRDRRFRIDSGTGL
                     LNGAPLKTQGTLYTDDQGRLGADLTAKIEGRFTKEEVFAFWPQDLAPQTRRMLIERIR
                     QGDFANADFTLKAPPGHFVADGLTNDDLRLDFDFSGLEVAIDPRMHDAEGLSGHGLLL
                     GDSFRMDVTGGRLIKVGLKRGRLSVPSFHDHRTRTLIALDAEGQAADVIEAIDPLADG
                     GLTQHGLTAQRLNGDAAAHVEISFPTLQEITDRNFSVVFDGHIANAGLKQAALGWDLS
                     GGELDVKGDLLADRLDIAGPARVGPFTGDIAYRTQFQPKTQAVDFKGRFNAAQFGGSP
                     RVPVGITGHFTITGGAGQGDVDADIFKGHVDWSGGEKDAQGRPDQVTIAGDTLRSGME
                     AQGLPIFEHLRPQLPTRITLLRSGDIWSGEIDAEALSGDIAYIEGERPRLVYKSTITP
                     LAARELGYGALPMFKAPRRLTVNIALDNDSKEALITLDAMKGVLGWKEIPGSDDVLRR
                     FDMTVTPDDWATLGLPTAFFHPKEDVPVTALWRQSERLLQGQVRLMGQSIDFDMPLHP
                     YGEVENPPPLIADAATPPPHVLQVRGQVTPDMLATLGYTQDPVSVDGPLGVVFTLF"
     gene            3134..3631
                     /locus_tag="VF402_00325"
     CDS             3134..3631
                     /locus_tag="VF402_00325"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7798760.1"
                     /translation="MAKPDKTGDFSPYPAPDFSAASSAGGTVARDDLKGRWTVLFLYP
                     RDDTPGCTQESCEFSAALADFRALGAQLYGLSKDGLKAHEKFIAKYGLAMPLLSDPDN
                     AAIAAFGSWIEKSLYGRKYMGTDRSTFIIDPEGRVRHVWRGVKVKGHVAEVLATLRTL
                     VSVRA"
     gene            3865..5010
                     /locus_tag="VF402_00330"
     CDS             3865..5010
                     /locus_tag="VF402_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7798761.1"
                     /translation="MTAVGEKNVDRLGDWMENVLPERHIYIRSHGETRAFTLTPGRQF
                     TLIFLTAALLTWLAISTGFTLFFAAAHGAGAESEVLMMKAQMERWIADRQARLDIATQ
                     QATNNSGSLEDLAKTVTARNDALVKVLSDFKGVPGAAAALAPAQIDDSLPPIERIYSV
                     RAEQERLVTRAEAFAKTRAERLRLAFRLAGLNPAAFSGGGGSPLASKDPASLAQFLGV
                     DVDFADRIHSAAADLSDMRGLQNSAGHLPFGRPTIGTRESSGFGVRIDPFTHTPHIHT
                     GQDFAGPMLTPVYATAPGIVSFTGVRTGYGNVVEIDHGNGFMTRYAHLAAYSVHPGQR
                     VALDQRIASMGSTGRSTGPHLHYEVWINGRPQNPARFLKAGDYVQQN"
     gene            4994..5569
                     /locus_tag="VF402_00335"
     CDS             4994..5569
                     /locus_tag="VF402_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7798762.1"
                     /translation="MFNKTKPSKPIAKPQPVPPPPLNDAPQVNMARTKPAATTPRPLS
                     SIGSGLLIDGNVTGNGDLHLDGAVRGDVKVSHLIVGESGNIEGKVEAETIEVRGRVVG
                     AIQGRQVKLQATAYVEGDITHDQIAIDVGAFFQGRCVQNRRVETPAAPIPAAATHIAE
                     AAPVTPAPITPAPVGTPSLDAYDLNALSDLK"
     gene            5717..6718
                     /locus_tag="VF402_00340"
     CDS             5717..6718
                     /locus_tag="VF402_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7798763.1"
                     /translation="MTVPDSKPLQARRPLTLAAFWFILCETLREWNDDKAPRLGAALA
                     FYSVLSLGPLLLIVIAVASLFFGRDAVSGYLIAEIQTLVGDRGAHAIQSILRHRQHAQ
                     DHSGGGWATAIGVITLLVSSTGFFAQLQDALNTIWNVDHIPRGHWIWFVKKRLLSFAM
                     ILGVGFLLLIGLVISAALSAFSAPLAVHTSPMTAHLANMVISFVVAAVLFAMIYKFLP
                     DVAIKWRDVAIGAVITALLFEIGKFLIGLYLGHSAFGSAYGAAGSVIVLLVWIYYSTQ
                     VFFLGAEFTQVYARYMGDEVVIKKGRRKMSVKADAHPHHSPDVVNVAKTEKRAPEPK"
     gene            complement(6719..>7081)
                     /locus_tag="VF402_00345"
     CDS             complement(6719..>7081)
                     /locus_tag="VF402_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX7798764.1"
                     /translation="KTDSAVRLTHIPTGIAVACQAGRSQHANRDEAWKMLRARLYELE
                     LQKREAAQAALEEQKTDIGWGHQIRSYVLQPYQMVKDLRTNVETSDSQGVLDGDLDAF
                     MGASLAQRVGLTRDEGAA"
BASE COUNT         1160 a         2480 c         2275 g         1166 t
ORIGIN      
        1 cgaacagggt gaagacgacg cccagcggcc cgtcgacgct caccggatcc tgcgtatagc
       61 cgagcgtggc cagcatgtcc ggcgtcacct gtccgcgcac ctgcaggaca tgcggcggcg
      121 gcgtggcggc gtccgcgatc agcggcggcg ggttttcgac ctcaccgtag gggtgcagcg
      181 gcatgtcgaa atcgatgctt tggcccatca gccggacctg cccctgcaac aggcgctcgc
      241 tctgccgcca cagggcggtg accggcacgt cctctttcgg atggaagaag gccgtcggca
      301 ggcccagcgt ggcccagtcg tccggcgtca ccgtcatgtc gaagcggcgc agcacgtcgt
      361 cgctgcccgg aatctccttc cagcccagca cgcccttcat cgcgtcgagc gtgatcagcg
      421 cctccttgct gtcgttgtcg agcgcgatat tgacggtcag gcggcgcggc gccttgaaca
      481 tcggcagcgc gccatagccg agttcgcgcg ccgccagcgg cgttatggtg gatttataga
      541 ccaggcgcgg acgctcgccc tcgatatagg cgatgtcgcc ggacagggcc tcggcgtcga
      601 tctcgccgct ccagatgtcg ccggagcgca gcagggtgat gcgcgtcggc agttgcggcc
      661 tgagatgctc gaagatcggc agcccctgcg cctccatgcc cgaacgcagg gtgtcgcccg
      721 ctatggtcac ctgatcgggg cgcccctgcg cgtccttctc gccgccggac cagtcgacat
      781 ggcccttgaa gatgtcggcg tcgacatcgc cctgaccggc gccgccggtg atggtgaaat
      841 ggccggtgat gccgaccggc acgcgcggcg agccgccgaa ctgggcggcg ttgaagcggc
      901 ccttgaaatc gacggcctgg gttttgggct ggaactgggt gcgataggcg atgtcgccag
      961 tgaacggccc gacccgcgcc ggtccggcga tgtcgagccg gtcggccagc aggtcgccct
     1021 tgacgtccag ctcgccgccg ctcagatccc agcccagcgc cgcctgtttc agcccggcat
     1081 tggcgatatg gccgtcgaag acgacgctga aattgcggtc ggtgatctcc tgcaaggtcg
     1141 ggaagctgat ctcgacatgg gcggcggcgt cgccgttcag ccgctgcgcc gtcagcccgt
     1201 gctgcgtcag cccgccgtcg gccagcgggt cgatcgcctc gatcacgtcc gccgcctgcc
     1261 cctcggcgtc cagcgcgatc agggtccggg tgcggtgatc gtggaagctg ggcacgctca
     1321 gccgtccgcg tttcaggccg accttgatca gccgcccgcc ggtcacgtcc atcctgaaac
     1381 tgtcgcccag cagcagaccg tgcccgctca ggccctcggc gtcgtgcatg cgcgggtcga
     1441 tggcgacttc gagcccgctg aaatcgaagt cgagccgcag gtcgtcattg gtcaatccgt
     1501 cggcgacgaa atgccccggc ggcgccttga gcgtgaagtc ggcgttggcg aaatcgccct
     1561 gacggatgcg ctcgatcagc atgcggcgcg tctgcggcgc cagatcctgc ggccagaagg
     1621 cgaacacctc ctccttggtg aagcggccct cgatcttcgc ggtcaggtcg gcgccgaggc
     1681 gtccctgatc gtcggtgtag agcgtgccct gcgttttcag cggcgcgccg ttcagcagcc
     1741 ccgtgccgct gtcgatgcgg aagcgccggt cgcgcggcac gtaccgcccg cggaaatgcg
     1801 cgtcggtcag ggtctgcggc gcatagtcgt cggccaggtt gccgcgcagc ctcggaccgg
     1861 aaaagtcgta ggcgatgacc atgttggcgc cggtcttgtc gttgtgcggc tggatgacga
     1921 tgcgacccga caggtcgcca tcgatgcgcg gcgaggtgat gcggaaggtg gtgaagcgcg
     1981 cggtgtgcga ggcggaatcg taggtgacgc gcacgcgcgc ctcctgaaag gtctggtgca
     2041 tatcgccgat gtcgagccgc ccggcgcccg ccgacacgtc gagccaggcg ttctcgaagc
     2101 cgcgcccttg cgtgtagttg atgcgcgcca gcccgctcac cggcgcgcgc accccggcca
     2161 gcagccgcgt cacgcccgag gacggcgcga cataggccgg atcgagatgg ctgaggctgg
     2221 cgctcagcac catgttcttc agcccgacct gcccctcggc ggcggcgcgc agcgtggcgg
     2281 cgggcgcgcc cgtcggcgcg atgctcagat cggcgtggct ggccagacgg ccgtgcagct
     2341 tggtgaaatc gacgcgcgcg acgtgggcgg tccagtcgac gcccatatgg cgcggatcgc
     2401 gcgcctgctc gatcaggcgc acctcgccgt tgcgcaagga cgcctgacgc acgaagctgg
     2461 ccgggcggct cagccgctcg cgcccggtca ggtcgtagaa gaacttgtcc agcccgcccg
     2521 tctccgacgg ggcgccgttc gcctcgtagc cgagatcgta gcgcccctcg cgcgacaccg
     2581 aggcggcgag gaagaagtcg tcggcggtca gccgcgcggg cgcgatgcgc gccatcaaca
     2641 gcccgtccgc cgccagcgcc atctccatgt gcccggcgcg cgccacgatg cgatggtgcg
     2701 aatcgaggat gcgcagatcc tgaaaacgga agccgaccgc gcgcgcgtcg ccgaaccaga
     2761 cgaggtcgag atggccgatg ctggcgtgcg tgcccggcag cgcgctttcc agccgcgaca
     2821 cgatcaccgg gcggatcgcg tcgacccgga tcggcgccgc cgccagccgc cacgccagca
     2881 ccaccagcat gaacagaacc caccccagca cgaacatccg caaccgacgc gccaaaacgt
     2941 cgaatacggg ccggacgggt gcaaaaaatt tcttcacgta aaggtctggc gaaaaggtct
     3001 gaaaacgcgg atgctctgtc gtacccgaaa cggacgaaaa cggcgttaac atgcgcgtcc
     3061 gcgcgtgtca tccgccgcct gatcttttcc acgggccgtt ttccccacct acgcgaattc
     3121 ataaggctaa cttatggcga aaccagacaa aaccggcgac ttttctccct atcccgcgcc
     3181 cgatttcagc gcggcctcca gcgcgggcgg aaccgtcgcg cgcgacgacc tgaaagggcg
     3241 ctggaccgtc ctgttcctct accccaggga cgacaccccc ggctgcacgc aggaaagctg
     3301 cgaattttcc gccgccctgg ccgatttcag ggcgctgggc gcgcagcttt acggcctgtc
     3361 gaaggacggg ctgaaagctc acgaaaagtt tatcgccaaa tacggcctcg ccatgccgct
     3421 gctgtccgat cccgacaacg ccgccatcgc cgccttcggc tcatggatcg agaaaagcct
     3481 atacggccgc aaatatatgg gcacggaccg atcgaccttc atcatcgacc ccgaaggccg
     3541 ggttcgccat gtctggcgcg gcgtcaaggt caagggacac gtcgcggagg ttctggcgac
     3601 gctcaggacg ctggtttccg tccgcgcgta aacaaatgtc gccgttcgcg gttaatgcgg
     3661 cggcggcatc gatcttgaaa ccccggtcat gccgttttca aatcgggaca cttccccgaa
     3721 aagtggaaat atcatgaccc gcgcgccgca caattcgcct gccagtcctt ttcaggaact
     3781 gagccgcgcc ctgcgcgccg cgccgcgctg tacagcccgt caagtccgtg tcaaaacgat
     3841 acagttgaac gggggtttgg cgcaatgacg gccgtaggcg aaaaaaacgt cgatcgtttg
     3901 ggcgactgga tggagaacgt cctgcccgaa cgccacatct acatccgctc gcacggcgag
     3961 acgcgggcct tcacgctgac gccggggcgg cagttcacgc tgatcttcct gacggcggcg
     4021 ctgctgacct ggctggcgat ctcgacgggc ttcaccctgt tcttcgccgc cgcccacggc
     4081 gcgggcgcgg aaagcgaagt gctgatgatg aaggcgcaga tggagcgctg gatcgccgac
     4141 cgtcaggcgc gcctcgacat cgccacccag caggccacca acaattccgg ctcgctggag
     4201 gatctggcca agaccgtgac cgcgcgcaac gacgcgctgg tcaaggtgct gagcgatttc
     4261 aagggcgtgc cgggcgcggc ggcggcgctg gcgcccgccc agatcgacga cagcctgccg
     4321 ccgatcgagc gcatctattc ggtgcgcgcc gaacaggagc gtctggtcac ccgcgccgaa
     4381 gccttcgcca aaacccgcgc cgagcgcctg cggctggcct tccggctggc cgggcttaat
     4441 ccggccgcct tctccggcgg cggcggcagc ccgctggcca gcaaggatcc ggccagtctg
     4501 gcgcaatttc tcggcgtcga cgtcgatttc gccgaccgca tccacagcgc cgccgccgac
     4561 ctgtcggaca tgcgcggcct gcaaaacagc gccgggcatc tgccgttcgg ccgcccgacc
     4621 atcggcacgc gcgaaagctc cggcttcggc gtgcgcatcg atccgttcac gcacaccccg
     4681 cacatccaca ccggccagga tttcgccggc ccgatgctga ccccggtgta cgccaccgcg
     4741 cccggcatcg tcagcttcac cggcgtgcgc accggctatg gcaatgtggt cgagatcgac
     4801 cacggcaacg gcttcatgac gcgctacgcc cacctcgccg cctacagcgt ccatcccggc
     4861 cagcgcgtcg cgctggacca gcgcatcgcc tcgatgggct caaccggccg ctccaccggc
     4921 ccgcacctgc actacgaggt gtggatcaac ggccgcccgc aaaaccccgc tcgcttctta
     4981 aaggcaggag actatgttca acaaaactaa gcccagcaag ccgattgcaa aaccgcaacc
     5041 ggtcccgccg cccccgctca acgacgcgcc gcaggtcaat atggcccgca cgaagcccgc
     5101 cgcgacgacg ccccgcccgc tgtccagcat cggctcgggc ctgctgatcg acggcaacgt
     5161 caccggcaac ggcgacctgc atctggacgg cgccgtgcgc ggcgacgtca aggtcagcca
     5221 cctgatcgtc ggcgaatccg gcaatatcga aggcaaggtc gaggccgaga ccatcgaggt
     5281 gcgcggccgc gtcgtcggcg cgattcaggg ccgtcaggtc aagttgcagg ccaccgccta
     5341 tgtcgagggc gacatcaccc acgaccagat cgccatcgac gtcggcgcct tcttccaggg
     5401 ccgctgcgtg cagaaccgcc gggtcgaaac gcccgcggcg cccattccgg ccgccgccac
     5461 gcacatcgcc gaagccgcgc cggtcacgcc cgcgccaatc acgcccgcgc cggtcgggac
     5521 gcccagcctc gacgcctacg acctgaacgc gctgtccgat ctgaaataac cggccgcgcg
     5581 acaatatgac acggtctcaa aaaagccggg aaagcgtctg atctttcccg gcttttgccg
     5641 tttttaccgc ttcctttttc gacagcggcg cgaaattcgt tatactgcgg cgcctttcgc
     5701 caaccgatga gccctcatga cggtccccga ctcgaaaccg ctacaggcca gacgcccgct
     5761 gaccctggcg gccttctggt tcatcctgtg cgagaccttg cgcgaatgga acgacgacaa
     5821 ggcgccgcgc ctgggggccg cgctggcctt ctacagcgtg ctgtcgctgg ggccgctgct
     5881 gctgatcgtc atcgcggtgg ccagcctgtt cttcgggcgc gacgccgtgt ccggctatct
     5941 gatcgccgag attcagactc tggtcggcga ccggggcgcg cacgcgatcc agtccatcct
     6001 gcgccatcgc cagcatgcgc aggaccattc cggcggcggc tgggccacgg ctatcggcgt
     6061 gatcaccctg ctggtctcct cgaccggctt cttcgcgcag ttgcaggacg cgctcaacac
     6121 catctggaac gtcgatcaca ttccgcgcgg ccactggatc tggttcgtca agaagcggct
     6181 tctgtccttc gccatgatcc tcggcgtcgg cttcctgctg ctgatcgggc tggtgatctc
     6241 ggcggcgctg tcggccttca gcgcgccgct ggccgtccat acctcgccca tgacggcgca
     6301 tctggccaat atggtcattt ccttcgtcgt ggcggcggtc ctgttcgcga tgatctataa
     6361 gttcctgccc gacgtggcga tcaaatggcg cgacgtcgcc atcggcgcgg tgatcaccgc
     6421 gcttctgttc gagatcggca agttcctgat cggcctctat ctcggccatt ccgccttcgg
     6481 ctcggcctac ggcgcggcgg ggtcggtgat cgtgcttctg gtgtggatct attattcgac
     6541 gcaggtcttc ttcctcggcg ccgagttcac gcaggtctat gcgcgctaca tgggcgacga
     6601 ggtcgtgatc aagaagggcc ggcgcaagat gtccgtcaag gccgacgcgc acccgcacca
     6661 ttcgcccgac gtggtcaacg tcgccaagac ggaaaagcgc gcgccggagc cgaaatgatt
     6721 acgccgcccc ttcgtcgcgc gtcaggccga cgcgctgcgc cagcgacgcg cccatgaagg
     6781 cgtcgagatc gccgtccaga accccttgcg aatccgaggt ttcgacgttg gtgcgcaggt
     6841 ccttgaccat ctgatacggt tgcagcacgt aggagcggat ctggtgcccc cagccgatgt
     6901 cggtcttctg ctcctccagc gcggcctgcg cggcctcgcg cttctgcaac tccagctcgt
     6961 acagccgcgc gcgcagcatc ttccacgcct cgtcgcggtt ggcgtgctgc gagcgcccgg
     7021 cctgacaggc gacggcgatg ccggtcggga tgtgcgtcag gcgcaccgcc gaatcggtct
     7081 t
//
LOCUS       DASVLL010000021         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_162276,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000021 DASVLL010000000
VERSION     DASVLL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162276"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /gene="hslV"
                     /locus_tag="VF402_00350"
     CDS             <1..530
                     /gene="hslV"
                     /locus_tag="VF402_00350"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX7798765.1"
                     /translation="PNWHGTTIVAVRHNGKTVIAGDGQVSMGATIVKGGAKKVRTLAN
                     GKVLVGFAGATADAFTLLERLEAKLELFPDQLARACVDLAKDWRTDRYLRRLEAMLLV
                     ADKHHVLTLTGVGDVLEPEDGVAAIGSGGAYALSAARALLEYETDAEIIARKAMKIAA
                     SICVYTNDHLTLERL"
     gene            527..889
                     /locus_tag="VF402_00355"
     CDS             527..889
                     /locus_tag="VF402_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798766.1"
                     /translation="MILVEDAIQRARLSVKMPATLILLALVGQALLLYPSGLLPKPLA
                     MTSLFLAILAWPLSLAYRAVAAPRWKLWAYPQVGNPAHLRDIAVGMRLIPAAGSAMEK
                     LEICSPEERRRILAYEGR"
     gene            1061..2362
                     /gene="hslU"
                     /locus_tag="VF402_00360"
     CDS             1061..2362
                     /gene="hslU"
                     /locus_tag="VF402_00360"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX7798767.1"
                     /translation="MTTFSPREIVSELDRHIIGHADAKKAVAVALRNRWRRKRTDAAI
                     RDEITPKNILMIGPTGVGKTEIARRLARLANAPFLKVEATKFTEVGYVGRDVDQIVRD
                     LVEAALLMVREKNRAGVRAKAEHQAEERLIDALVGATAAAATRESFRRKLRDNELDER
                     EIEIALTETGAPIGFMEIPGQQAPGAFNLGEMLSKAMGNRQKTVKTTVKAAWPSLITE
                     ESDKLLDQDSLTLEALKLAENEGIVFLDEIDKVASRQDRGGADVSREGVQRDLLPLIE
                     GTTVSTKYGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPIRVELKALNEDDFRRIL
                     SEPEANLIKQNQALMATEGVTLTFDDGAIAAMAQAAATVNAKVENIGARRLHTVIEKV
                     MEDLSFTAADMTGQTVAIDAAYVKQRLGEIAGDADLSRYIL"
     gene            2600..>3166
                     /locus_tag="VF402_00365"
     CDS             2600..>3166
                     /locus_tag="VF402_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7798768.1"
                     /translation="MSDPLSPDSAPFWRQHVPAESLGWSPVRSHVGDGRIGRDASVGE
                     LRELWGRAPLIVANAALIRTRLFANRPHREARIFDVLELFAFVRPAQFCAPSAAGLAL
                     ACGFDEPETPQDASRALFDVIDSLVRTLTTAPLGFRLAAASGVKLMEKQGWAWAPLLN
                     AALAETDMPSDYAPPPALDVWTLLEEWED"
BASE COUNT          521 a         1060 c         1061 g          524 t
ORIGIN      
        1 tccccaactg gcacggcacg accatcgtcg ccgtgcgtca taacggcaag acggtgatcg
       61 cgggcgacgg tcaggtgtcg atgggcgcca ccatcgtcaa gggcggcgcg aagaaggtgc
      121 gcacgctggc caatggcaag gtgctggtcg gcttcgcggg cgcgaccgcc gacgccttca
      181 ccctgctgga gcggctggag gccaagctgg agctgttccc ggaccagttg gcgcgcgcct
      241 gcgtggacct cgccaaggac tggcgcaccg accgctatct gcgccgtctc gaagccatgc
      301 tgctggtggc ggacaagcat catgtgctga ccctgaccgg ggtgggcgac gtgctggagc
      361 cggaggacgg cgtggccgcc atcggttcgg gcggcgccta cgcgctgtcg gcggcgcgcg
      421 cgctgctgga atacgaaacc gacgccgaga tcattgcgcg caaggcgatg aagatcgccg
      481 cgtcgatctg cgtttacacc aacgatcatc tgacattgga gcgcctgtga ttctcgtcga
      541 ggacgccatc cagcgcgcgc ggctcagcgt caagatgccc gcgaccctga tcctgctggc
      601 gctggtcggg caggcgctgc ttctgtaccc cagcggcctg ttgcccaagc cgctggctat
      661 gacgtcgctg tttctggcca tactggcctg gccgctcagc ctcgcctacc gcgccgtggc
      721 ggcgccgcga tggaagctgt gggcctatcc gcaggtcggc aatccggccc atttgcgcga
      781 catcgccgtc ggcatgcggc tgatcccggc ggcgggcagc gcgatggaaa agctggagat
      841 ctgctcgccg gaggaacggc ggcggatttt ggcttatgaa ggcagatgat ggcccgaaaa
      901 cacgaaggag cacgaaaatc atgtgtacgt gccgatcctt ctccgcaacc cgtaacgcat
      961 tctgaagcgc tttgcgcttc gttattttca agcctcgaaa cctgaaaacg cctttcgtgt
     1021 tttttcgcgt ctttcgtggc caaacccctt gagtcccctc atgaccacat tctccccgcg
     1081 tgaaatcgtc tccgaactgg accgtcacat catcggccat gccgacgcca agaaggcggt
     1141 cgccgtcgct ctgcgcaacc gctggcggcg caagcggacc gacgcggcga tccgcgacga
     1201 gatcacgccg aagaacatcc tgatgatcgg cccgaccggc gtcggcaaga ccgagatcgc
     1261 ccgccgtctg gcccggctgg ccaacgcgcc cttcctcaag gtcgaggcga ccaagttcac
     1321 cgaggtcggc tatgtcggcc gcgacgtcga tcagatcgtg cgcgatctgg tcgaagccgc
     1381 cctcctgatg gtgcgcgaga agaaccgcgc cggggtgcgc gccaaggcgg agcatcaggc
     1441 cgaggagcgc ctgatcgacg ctctggtcgg cgcgacggcg gcggcggcga cccgcgagtc
     1501 gttccgcagg aagctgcgcg acaacgaact ggacgaacgc gagatcgaaa tcgccctgac
     1561 cgagaccggc gcgccgatcg gcttcatgga gattccgggc cagcaggcgc cgggcgcctt
     1621 caacctcggc gagatgctgt ccaaggcgat gggcaaccgg caaaagacgg tgaaaaccac
     1681 cgtcaaggcg gcgtggccca gcctgatcac cgaggagtcg gacaagctgc tcgatcagga
     1741 ttcgctgacc ctcgaagcgc tgaaactggc cgagaacgag ggcatcgtct ttctcgacga
     1801 gatcgacaag gtcgcctcgc gtcaggatcg cggcggcgcg gacgtgtcgc gcgagggcgt
     1861 gcagcgcgac ctgctgccgc tgatcgaagg cacgaccgtc tcgaccaaat atggcccggt
     1921 caagaccgac cacatcctgt tcatcgcctc gggcgcgttt catgtcgcca agccgtccga
     1981 cctcttgccc gaattgcagg gccgcctgcc gatccgcgtc gagctgaagg cgctgaacga
     2041 ggacgatttc cgccgcatcc tcagcgagcc ggaggccaac ctgatcaagc agaatcaggc
     2101 gctgatggcg acggagggcg tgaccctgac cttcgacgac ggcgcgatcg ccgcgatggc
     2161 gcaggccgcc gccacggtca acgccaaggt cgagaacatc ggcgcccgcc ggctgcatac
     2221 ggtgatcgag aaggtgatgg aggacttgag cttcaccgcc gccgatatga ccggtcagac
     2281 cgtggcgatc gacgcggcct acgtaaagca gcgcctcggc gagatcgcgg gcgacgccga
     2341 cctgagccgg tatattttgt agcgcgctgt tcttgccctc gggtgagggg ccgcctgccg
     2401 accgggcgca aacgtcgaca gcccctcagg ctgcgcgccc cgcccgtcgc cttcgcctga
     2461 cggctcggct ctcgtcctct cccctcgtgg agggggagag gggacttaaa gtcggcgtaa
     2521 gcttttctcc ccccctgagc gtcgccgcct ccgcaagctt gatttccctt tccggcggtt
     2581 ctgttctagg ctcccctccg tgtccgatcc gctttctcct gattccgcgc cgttctggcg
     2641 tcagcatgtt cccgccgaat cgctcggctg gtcgccggtg cgcagccatg tcggggacgg
     2701 gcgcatcggt cgcgacgcat cggtgggcga gctgcgcgag ctttgggggc gcgcgccgct
     2761 gatcgtcgcc aacgcggcgc tgatccgcac ccggctgttc gccaaccgtc cgcaccgcga
     2821 ggcgcggatt ttcgacgtgc tggagctgtt cgccttcgtg cgtccggcgc agttctgcgc
     2881 gccgtcggcg gcggggctgg ccctggcctg cggcttcgac gagccggaga cgccgcagga
     2941 cgcgtcgcgc gcgctgttcg acgtgatcga cagcctcgtc cgtacactga cgaccgcgcc
     3001 gctcggcttc cggctggcgg cggccagcgg cgtgaaactg atggaaaagc agggctgggc
     3061 gtgggcgccg ctgctgaacg cggcgctggc cgaaaccgac atgccgtccg actacgcgcc
     3121 gccgcccgcg ctcgacgtct ggaccctgct ggaggagtgg gaggac
//
LOCUS       DASVLL010000022         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_215968,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000022 DASVLL010000000
VERSION     DASVLL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215968"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF402_00370"
     CDS             complement(<1..369)
                     /locus_tag="VF402_00370"
                     /inference="COORDINATES: protein motif:HMM:NF020302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HEX7798769.1"
                     /translation="MEEAIFFYTPDGPFGDFSNFAKFGVALDGVWWPTTEHYFQAQNF
                     GDPAYQEKTRRASTPKQAADLGRTRALALRPDWDVARVDVMRRVVTTKFETHEALRVL
                     LLSTGDRPLVESAPGDNFWGA"
     gene            909..>1709
                     /locus_tag="VF402_00375"
     CDS             909..>1709
                     /locus_tag="VF402_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7798770.1"
                     /translation="MAWAPSADAAAASRPLRTRANFNADWRFRLGTVDGGERPDFDDR
                     SWEHVGLPHSFSIPYFRAPDFYTGDGWYRKSFVIPSGPPGRRYSLEFEGAFQDARVYV
                     NGVELAHHRGGYTGFPVDITPAARPGRNVVAVKVNNDWSATLAPRAGEHVFSGGLYRD
                     VWLVSTDAVHVPWTGARVTTPDLSAASGKVRVDTEVSNSGAVPVEVTVDTRIVDDKGA
                     VVATLPKGHVRLAPGGMRIVSQLSAPIEKPRLWSPDTPVLYRAVTRLSV"
BASE COUNT          319 a          538 c          539 g          313 t
ORIGIN      
        1 ggcgccccaa aaattatctc ccggtgcgct ttccactaac gggcggtctc cggtcgacag
       61 aagaagaacc cgcaaagcct cgtgggtctc gaatttggtg gtcacaacac gtcgcatgac
      121 atccacccgc gccacatccc agtcaggtcg gagcgcaagc gcacgcgtcc gtccgaggtc
      181 ggccgcctgt ttgggcgtag atgcgcgacg tgttttctcc tggtaagcag gatcgccaaa
      241 gttctgtgcc tgaaaataat gttcagttgt aggccaccat acgccgtcca gcgctacgcc
      301 gaatttagcg aagttggaga aatcgccgaa cgggccgtcc ggggtgtaga agaaaatcgc
      361 ctcttccatc gtcgtcaact cttaggcctc tctgatcgcc agcgggtgag cctacccaca
      421 attcgacatg acaaatagct gccgagtaag tcaggtttgg acgcatttcg atatcttgcg
      481 cgcgaagcgg acactacgga acaatccagg cgcgattcgc gcgcgtcgaa ggcccggatc
      541 tattgggcgt ttggaacagc ggacacgagc gtaccggcga tttcaagcga aatgctcgaa
      601 gccgccggga aaaccgtcac ggcccgccgc gttcccgacg ccgggcatgg cctgaggccg
      661 ctcgacagca atgattgggc gcctgtcgat aaggaataca gggcggccct gagctggttc
      721 tggatgaagc ctttataggg acgacgcgca ctcaacactt ctatcatata tagaatacag
      781 ttccatatat gccagttgta ggctatggtc ccggcaaccg acccataaaa atgcccggag
      841 gaaccatgcg tgtgcgtttg caagcccttt ttgcaattgt ggccgccgcg cttttgacgt
      901 tgggcgccgt ggcatgggcg ccgtcggcgg acgccgccgc cgcgtcccgg ccccttcgga
      961 cgcgcgccaa tttcaacgcc gactggcggt tcaggctcgg cacggtggac ggcggcgagc
     1021 ggccggactt cgacgaccga tcatgggaac atgtcggcct gccgcacagt ttcagcatcc
     1081 cctatttccg ggcgccggac ttctataccg gcgacggctg gtaccgaaag agcttcgtca
     1141 ttccgtcagg gccgcccggc cgccgctatt cgcttgaatt cgagggcgct tttcaggacg
     1201 cgcgcgtcta tgtcaacggc gtggagctgg cgcatcaccg cggcggctac accggctttc
     1261 ccgtcgacat cacgccggcg gcgcggccgg gccgcaacgt cgtcgcggtc aaggtcaata
     1321 acgactggag cgcgaccctg gcgccgcgcg cgggcgaaca cgtttttagc ggcggcctgt
     1381 accgcgacgt ctggctggtg tcgaccgatg cggtgcatgt cccctggacc ggcgcgcgcg
     1441 tgacgacgcc cgacctttcg gcggcctccg gcaaggtcag ggtcgacacc gaggtcagca
     1501 acagcggcgc cgttccggtc gaagtcacgg tggacacgcg catcgtcgac gacaagggcg
     1561 cggtcgtcgc gaccctgccc aagggccatg tgcgactggc gccgggcggg atgcggatcg
     1621 tgagccagct atcggcgccg attgaaaaac cccgtctgtg gtcgccggac acgccggtcc
     1681 tttaccgcgc ggtgacgcgg ctgagcgtc
//
LOCUS       DASVLL010000023         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_1268, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000023 DASVLL010000000
VERSION     DASVLL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..723
                     /locus_tag="VF402_00380"
     CDS             106..723
                     /locus_tag="VF402_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX7798771.1"
                     /translation="MAKIDLKTEYKSLYHAGTRDFALIEVPPLSYLMVDGEGDPNTAP
                     AYAAAVEALYALSYTLKFMSRAAFGRDYVVGPLEGLWWAKDMTAFTRRDKSAWSWTMM
                     ILQPDWIAPEHVRAAKGEVLMKKGLPGLEKARLETLEEGLCAQILHVGPYDDEGPALQ
                     RLHQAWLPAHGLKPRGRHHEIYLSDPRKVAPEKCKTLLRQPVRPI"
     gene            778..3141
                     /locus_tag="VF402_00385"
     CDS             778..3141
                     /locus_tag="VF402_00385"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEX7798772.1"
                     /translation="MRLALLPLLLCLLPGAASAANWISDRPQPGGMALNGAAVVYDPN
                     DAAVVAIAARDLAADIGRVTGHAAPVNTGGARQIWIGVAGHSAPIDALGAKLDLSKLN
                     ACWECYVIAVVDHPKPGVDQALVIAGSDRRGAAYGAYEASRAIGVSPWVWWADVAPKH
                     REALYVAAGARRFGPPSVQYRGLFINDEDWGLEPWAAKTFDPAYGNIGPKTYERVFDL
                     MLRLRANTLWPAMHEVSKPFNLDPANAALADRYAIVMGSSHAEPMLRNNVGEWTDAPG
                     NYDYATHPDEVRDYWRQRLKTNGRYENIYTLGMRGIHDSAMVGAKTTEEKRALLNRIL
                     HDQRQLLADEVGPPQTLPQTFVPYKEVLDVYRTGLDLPADVTIMWPDDNFGYIRQFPD
                     AKERQRSGGSGVYYHLEYLGAPVSYIWLSTTPPALIGEEMGRAYDNGARRMWIFNVGD
                     IKPAEIDMSYAFDLAWNVAATRKLTQKQWLTGFAGETFGPEQAARLGDVLDRYYHLNF
                     ERRPEQLQWYWGKGAVPKASPLSAAAIDARVAAFDALRRDADAMVPHSDAWFELVGYP
                     VAASALANRRYFALECAETGKPCAVSGEAAARAADAALTALGQRYDRLQDGKWKGLIN
                     EEPADKQWSFRTAPPPWPGTKQALGPAPVETVKALPLTPVKGWTRIEGMGRSGFVLRA
                     EDKAPLVTQANLQGAWSLRVAILPIFPADGAKAWRLGVSVNGGPARTLTYARDALDKD
                     WEAGVMANRLVQDAGEISGPVTIRITPLDPGVIVESVEAVREGKAAD"
BASE COUNT          585 a         1045 c         1089 g          530 t
ORIGIN      
        1 acttttgggg ccgagatgcg cgtcaaagca aaataagctt aatgtgctgc tgacgcggat
       61 tgtcgcgcag ggctgtagcc tgcttccatc acagggagcc gcgttatggc gaaaatcgac
      121 ctcaagaccg aatacaagag cctctaccac gccgggacga gggacttcgc gctgatcgag
      181 gttccgccgc tcagttatct gatggttgat ggcgagggcg atccgaacac ggcgcccgcc
      241 tacgccgccg ccgtcgaagc cttgtacgcc ctgtcgtaca cgctgaaatt catgagcagg
      301 gcggcgttcg ggcgcgacta cgtggtcggt ccgctggagg gcttatggtg ggcgaaggac
      361 atgacggcgt tcacgcgccg ggacaagtcc gcgtggtcgt ggaccatgat gatcctgcaa
      421 ccggactgga tcgcgccgga gcatgtgcgc gccgccaagg gcgaggttct gatgaagaag
      481 ggcctgccgg gactggagaa ggcgcgtctg gagacgcttg aggaagggtt gtgcgcgcag
      541 attctgcatg tcgggccgta cgacgacgaa ggcccggcgc tgcaacggct gcatcaggcg
      601 tggctgcccg cgcatggcct taaaccgcgg ggccgccatc acgaaatcta tctcagcgat
      661 ccgcgcaagg tcgcgccgga aaagtgtaag acccttttgc gtcagccggt cagacccatt
      721 tgactccgcg cgccgcgcgt cgtaatcgtt tccgatcaac actgatcgga aattgtcatg
      781 cgactcgccc tgcttccgct gctgctgtgc ctgctgccgg gcgcggcctc ggcggcgaac
      841 tggatttcgg acaggccgca gcccggcggc atggcgctga acggcgcggc cgtcgtttac
      901 gatccgaacg acgccgcggt ggtggcgatc gcggcgcgcg atctggcggc ggatatcggg
      961 cgcgtcaccg gccatgccgc gccggtcaat acgggcgggg cgcggcagat ctggatcggc
     1021 gttgcgggcc acagcgcgcc gatcgacgcc ttgggcgcga agctcgacct gtcgaagctg
     1081 aacgcttgct gggaatgtta cgtcatcgcc gtggtcgatc acccgaagcc gggcgtcgat
     1141 caggcgctgg tcatcgccgg gtccgaccgg cgcggcgcgg cctacggcgc ctatgaagcg
     1201 tcgcgcgcca tcggcgtgtc gccgtgggta tggtgggccg atgtcgcgcc gaagcatcgc
     1261 gaagccctct atgtcgcggc gggcgcgcgc cgcttcggcc cgccctcggt gcaatatcgc
     1321 ggcctgttca tcaatgacga ggactggggg ctggagccgt gggcggcgaa aaccttcgat
     1381 ccggcctatg gaaatatcgg cccgaaaacc tatgagcgcg tgttcgacct gatgctgcgg
     1441 ctgcgcgcca ataccttgtg gcccgccatg cacgaagtgt ccaagccgtt caacctcgac
     1501 ccggccaatg cggctttggc cgaccgctac gccatcgtca tgggctcgtc gcacgccgag
     1561 ccgatgctga ggaacaatgt cggcgaatgg acggacgcgc cgggcaatta cgattacgcc
     1621 actcacccgg acgaggtgcg cgactactgg cgtcaacggc tgaaaaccaa tggccgttac
     1681 gagaacatct acacgctggg catgcgcggc atccacgatt cggcgatggt gggcgccaag
     1741 acgaccgagg aaaaacgggc gctgctgaac aggattctgc acgatcagcg ccagttgctg
     1801 gccgacgagg tcgggccgcc gcagaccctg ccgcagacct tcgtgccgta caaggaggtg
     1861 ctggacgtct atcgcaccgg cctcgacctg cccgccgacg tgacgatcat gtggccggac
     1921 gacaatttcg gctatatccg ccagttcccg gatgcaaaag agcgccagcg ttccggcggc
     1981 tccggcgtct attaccacct cgaatatctc ggcgcgccgg tcagctatat ctggctgtcg
     2041 accacgccgc ccgctttgat cggcgaggag atgggccgcg cttacgacaa cggcgcgcgc
     2101 cgcatgtgga tcttcaatgt cggcgacatc aagcccgccg agatcgacat gtcctacgcc
     2161 ttcgatctgg cgtggaacgt ggcggcgacg cgcaagttga cgcagaagca atggctgacc
     2221 gggttcgcgg gcgaaacctt cgggcctgaa caggccgcgc gcctcggcga cgtgctggac
     2281 cgctattacc atttgaattt cgagcgccgc cccgaacaac tgcaatggta ctggggcaaa
     2341 ggcgcggtcc cgaaggccag cccgctcagc gccgccgcca tcgacgcgcg cgtggcggcc
     2401 ttcgacgccc tgcgccgcga cgccgacgcg atggttccgc acagcgatgc gtggttcgag
     2461 ctggtcggct atccggtcgc ggccagcgcc ttggccaacc gccgctattt cgcgctggaa
     2521 tgcgccgaga cgggcaagcc gtgcgccgtc tccggcgaag ccgccgcccg cgccgccgac
     2581 gccgcgctca ccgccctggg gcaacgctac gaccgtttgc aggacggcaa atggaagggc
     2641 ctgatcaacg aggagcccgc ggacaagcaa tggtcgttcc gcaccgcgcc gccgccgtgg
     2701 ccgggcacga aacaggccct cggccccgcg ccggtcgaga cggtgaaagc cttgcccctg
     2761 acgcctgtca agggctggac gcgcatcgaa ggcatggggc gctcaggctt cgtgctgcgc
     2821 gccgaggaca aggcgccgct cgtgacgcag gccaatttgc aaggcgcatg gagcctgcgc
     2881 gtcgccatcc tgccgatctt cccggcggac ggcgcgaagg cgtggcggct cggcgtctcg
     2941 gtcaacggcg gcccggcgcg aaccctgacc tatgcccgcg acgcgctcga caaggactgg
     3001 gaagccggcg tcatggccaa ccggctggtt caggacgcgg gcgagatcag cggtcccgta
     3061 acgatccgca tcacccccct cgatccgggc gtcatcgtcg aaagcgtcga ggcggtgcgg
     3121 gaaggcaagg ccgcagattg acgcggcgtg gcgtcgcgcc acccaaagcc aagatactgc
     3181 aatcattact ttcggaataa attttgaata atccgggtta ttaagcgtgc agctatcaaa
     3241 ccgaaaaat
//
LOCUS       DASVLL010000024         4185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_216058,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000024 DASVLL010000000
VERSION     DASVLL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216058"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VF402_00390"
     CDS             <1..622
                     /locus_tag="VF402_00390"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7798773.1"
                     /translation="SAHYVVDEDGSVLQLVDEDKRAWHAGRSHWRQWDNVNNRSIGIE
                     IVNPGHEFGYRDFPDAQIDAVIGVLDGVRTRWEIPDHRILGHSDIAPARKQDPGERFP
                     WARLAEHGHGLWVRPDLPPDGVMGPPLGPGDEGLGVFSLQGALGKLGYNILPGGPYDD
                     ETATVVTAFQRHWLPDQIGSELQGRADARLRVTLMALLRHIALLEV"
     gene            646..981
                     /locus_tag="VF402_00395"
     CDS             646..981
                     /locus_tag="VF402_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798774.1"
                     /translation="MLSVAGVWGLFGVVMLAAGAHAPRMGIMATGGMMLLFHAAAAIG
                     LIRTPLLSGWRRALPVALMLLGSGLFALEVGLHAALGVTPFVMLAPVGGTIAILGWLA
                     LIVTPQLEP"
     gene            1016..1413
                     /gene="rnpB"
                     /locus_tag="VF402_00400"
     ncRNA           1016..1413
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF402_00400"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1414..2085)
                     /locus_tag="VF402_00405"
     CDS             complement(1414..2085)
                     /locus_tag="VF402_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX7798775.1"
                     /translation="MDNDHKDESSQENFSLKAQFSERLNLLLRRKGLTQAELSRLTGE
                     GASKINEYSKGRILPSAMTAIALAEALDVSVPFLMLGRAGTADAAQSVPILDASRVLP
                     DSGRDVQLYTAQMTLDDHLLRQVGRATTEGLIMARAEGDSMAPVITDGAHVLIDRLDR
                     RLREGVFAIRIGDDLRIKRLRPVGVGGVEAISDNAIYPPERFEGEMLAAFSIIGRALW
                     TGTRL"
     gene            2179..2604
                     /locus_tag="VF402_00410"
     CDS             2179..2604
                     /locus_tag="VF402_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798776.1"
                     /translation="MQGTSINLDSHGETLARRSRRRLNDAQIAEIAALRERGWGTRAL
                     ALRFGVTVSLVEWHCLRLGADAPKGGYAPRAVPEQPRGYLRAGRPVRLYTRDEDARLL
                     AMAREGRPERQIAEALQRSATSVAGRLMVLARREARLEG"
     gene            2899..3354
                     /locus_tag="VF402_00415"
     CDS             2899..3354
                     /locus_tag="VF402_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7798777.1"
                     /translation="MFLSSHEKPLDAKRRLLVPQDFRASALIPHDGVEPFEGLYCFAA
                     IDAACIECGGAAFFAGYRAVIDAIPKFTPARKAMEHRIYAAMQRLSFDTAGRITLPEA
                     LCERFNLNGNVLIAGLGDSFQIWSPDAYKTYMAEQEALVSAAFTGAGGL"
     gene            3356..>4185
                     /gene="rsmH"
                     /locus_tag="VF402_00420"
     CDS             3356..>4185
                     /gene="rsmH"
                     /locus_tag="VF402_00420"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7798778.1"
                     /translation="MAQDAHVSVLLPEVLDALGDVSGRLVVDGTFGAGGYTRALLEHG
                     ANVVAFDRDARVRPFADAVARDYPQGFRWVNRRFSEMAEGLAELGETACDAIVLDIGV
                     SSMQLDQAERGFSFMRDGPLDMRMSVDGRSAADIVNEDDRDEIARILWLYGEERRSRA
                     IAAAICRRREEKPFTHTLDLAETIEKALGGRKWIKGRGAPVHPATRSFQALRIAVNDE
                     LGELEAALATAETLLRPGGVLAVVTFHSLEDRIVKTFLNARAGRVPAASRHAPQAARA
                     "
BASE COUNT          655 a         1340 c         1480 g          710 t
ORIGIN      
        1 gtcggcgcat tatgtcgtgg acgaggacgg gtcggtcctc cagctcgtcg acgaggacaa
       61 gcgcgcctgg cacgcgggcc gctcgcactg gcggcagtgg gacaacgtca acaaccgttc
      121 gatcgggatc gagatcgtca atcccggcca cgaattcggc tatcgcgact ttcccgacgc
      181 gcagatcgac gcggtgatcg gcgtgctgga cggcgtgcgc acgcgctggg agattcccga
      241 ccaccgtatt ctcggccatt ccgacatcgc cccggcgcgc aagcaggatc ccggcgagcg
      301 cttcccgtgg gcgcggctgg ccgaacacgg ccacggctta tgggtaaggc ccgacctgcc
      361 gccggacggc gtgatggggc cgccgctggg gccgggcgac gaagggctgg gcgtgttctc
      421 gctgcaaggc gcgttgggca agctgggcta taacatcctg cccggcgggc cgtatgacga
      481 cgagacggcc acggtcgtca ccgcgtttca gcgccactgg ctgccggatc agatcggttc
      541 tgagcttcag ggccgggccg acgcgcgcct gcgcgtgacg ctgatggcgc tgctgcggca
      601 cattgctttg ctggaggttt aaggatggaa cgcggacaac gcatcttgct ctccgtcgcc
      661 ggcgtctggg gcctgttcgg cgtcgtcatg ctggcggcgg gggcgcacgc gccgcgcatg
      721 ggcattatgg cgaccggcgg catgatgctt ttgtttcatg cggcggcggc gatcggcctg
      781 atcaggacgc cgctgctcag cggttggcgg cgcgcgctgc cggtggcgct gatgctgctg
      841 gggtcgggcc tgttcgcgct ggaggtcggg ctgcatgcgg cgctcggcgt cacgcccttc
      901 gtcatgctgg cgcccgtcgg cgggacgatc gccatcctcg gttggctggc cctgatcgtg
      961 acgccgcagc ttgagccttg attttaccag cgtttgcgtc cacaagggcg gcggaccaga
     1021 cggacgggcg atcgcaggac cgctttgcgg cggcttgagg aaagtccggg ctccacggta
     1081 atatggcggc gggtaacgcc cgccgggggc gaccccaggg acagcgccac agagagcaga
     1141 ccgccgcgct tcggtgcggc aagggtgaaa gggtgcggta agagcgcacc gcgttcccgg
     1201 taacggcgaa cggcatggca agccccgtca ggagcaagac caaataggaa cggcgcgtcc
     1261 gcttcggcgg acagggcatt accgccccgg accgttcggg taggttgcgt gagccgtcca
     1321 gcaatggccg gccaagagga atgatcgcca aggcggtccc gatcctggat ccgggccgct
     1381 gaacagaacc cggcttacag tccgtctggt ccgttataat ctcgtgcccg tccacaaggc
     1441 gcggccgatg atgctgaacg ccgccagcat ctcgccttcg aagcgctcgg gcggatagat
     1501 ggcgttgtcg gaaatcgcct cgacgccgcc gacgcccacg ggccgcagcc gcttgatgcg
     1561 caggtcgtcg ccgatgcgga tcgcgaacac cccctcgcgc aggcggcggt ccagccggtc
     1621 gatcagcaca tgcgcgccgt cggtgatcac cggcgccatc gaatcgccct cggcccgcgc
     1681 catgatcagc ccctccgtcg tcgcgcgtcc gacctgacgc agcagatggt cgtccagcgt
     1741 catctgcgcc gtgtagagct gcacgtcgcg tccgctgtcc ggcaggacgc ggctggcgtc
     1801 gaggatcggc acgctttgcg ccgcgtccgc cgtcccggcg cggcccagca tcaggaacgg
     1861 caccgacacg tccagcgcct cggccagcgc gatggcggtc atggcggacg gcaaaattcg
     1921 accttttgaa tattcgttga tcttggacgc gccctctccg gtcaggcggc taagctccgc
     1981 ctgggtcagg cccttacgac gcaacaaaag attgagtcgt tccgaaaatt gcgcttttag
     2041 cgagaaattt tcttgactgg attcgtcttt atggtcatta tccattttta cggatatccc
     2101 attcgctttt ctggttgttg gcgtatccgg gcggagcgtc ggccgtggcg acgcaggggc
     2161 gcatgaggag ggacatgtat gcagggtaca tccataaatc tggattcgca cggtgaaact
     2221 ttggcccgcc ggtcgcggcg gcggctgaac gacgcgcaga tcgccgaaat cgccgccctg
     2281 cgcgagcggg gatggggcac gcgcgcgctg gcgctgcgct tcggcgtgac cgtcagcctc
     2341 gtggagtggc actgcctgcg gctgggggcc gacgcgccga agggcggtta tgcgccgcgc
     2401 gccgtgccgg agcagccgcg cggttatctg cgcgctgggc gtccggtgcg gctctatacg
     2461 cgcgacgagg atgcgcgcct gctggcgatg gcgcgggaag gacggcccga acgccagatc
     2521 gccgaggctt tgcaacgcag cgcgacctcg gtggccgggc ggctgatggt gttggcgcgg
     2581 cgcgaggcgc gccttgaggg ctgatcccgg cgccgccgcc gggatcggaa gaaggcggaa
     2641 gggcaggggg ccgcccttcc gccttcgctg tttcccggag gcggctcagg taaccgtgtt
     2701 cgttgtgtgt tctatccaca gggcgtggat aagtagaaaa cgaggtttaa aactcaattt
     2761 ggagtcattt agagtcattg catcccactt aatcccatgt taaccctgac cagcccgagt
     2821 gatttggccg gaggggccta gattgcgcgt tcgggcgcgt gaacagggag cgggcctgaa
     2881 aggcgcggca agcgggcggt gtttctctcc agccatgaga agcctttgga tgcgaagcgg
     2941 cggcttctgg tgccgcagga cttccgcgcg tccgcgctga tcccgcatga cggggtcgag
     3001 ccgtttgagg ggctgtactg cttcgcggcc atcgacgcgg cctgcatcga atgcggcggc
     3061 gcggcgtttt tcgccggtta tcgcgccgtc atcgacgcca ttccgaaatt caccccggcg
     3121 cgcaaggcga tggagcatcg catctacgcc gccatgcagc gcctgagctt cgacaccgcc
     3181 ggacgcatca ccctgcctga ggcgctgtgc gagcgcttca acctgaacgg caacgtgctg
     3241 atcgcggggc tgggcgactc gttccagatc tggagcccgg acgcctacaa gacctatatg
     3301 gccgagcagg aggcgctggt cagcgccgcc ttcacgggcg cgggagggct ttaggatggc
     3361 gcaagacgcg cacgtctccg tcctgctgcc cgaagtgctg gacgcgctgg gcgatgtgtc
     3421 gggcaggctg gtggtcgacg gcaccttcgg cgcgggcggc tatacgcgcg ccctgctcga
     3481 acacggcgcc aacgtcgtcg ccttcgaccg cgacgcccgc gtcaggccgt tcgccgacgc
     3541 ggtggccagg gattatccgc aaggtttccg ctgggtgaac cggcgctttt ccgagatggc
     3601 cgaggggctg gccgaactgg gcgaaacggc ttgcgacgcc atcgtgctgg acatcggcgt
     3661 gtcgtccatg caactcgatc aggccgagcg cggcttctcc ttcatgcgcg acgggccgct
     3721 ggacatgcgc atgagcgtcg acgggcgcag cgccgccgac atcgtcaacg aagacgaccg
     3781 cgacgagatc gcgcgtatcc tgtggctgta cggcgaggag cgccggtcgc gcgccatcgc
     3841 cgccgccatc tgccgccgcc gcgaggaaaa gccgttcacg catacgctcg atctggccga
     3901 aacgatcgaa aaagcgctgg gtggcagaaa atggataaag ggacgcggcg ccccggtcca
     3961 tccggcgacg cgcagctttc aggcgctgcg catcgcggtc aatgacgaac tgggcgagct
     4021 ggaggcggcg ctggcgacgg cggagactct gctgcggccc ggcggcgtgc tggccgtggt
     4081 caccttccat tcgctggagg accgcatcgt caagaccttc ctgaacgcgc gcgccgggcg
     4141 cgtccccgcc gcctcgcgcc acgcgccgca ggccgcgcgc gccga
//
LOCUS       DASVLL010000025         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_270266,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000025 DASVLL010000000
VERSION     DASVLL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270266"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="VF402_00425"
     CDS             complement(<1..1610)
                     /locus_tag="VF402_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7798779.1"
                     /translation="MRIFKAILLASAALTLLSGPAFAQSQKPTEVVVRGEKRVKPVDA
                     PPPLELYANMPRLEEVALSPDGDRVAFLTRIGDTRVLAAYRFSDKSHQIFRVEGGPAS
                     ALSWADNTHVLISDSRLALRGTCQNAPSGPGAKPNARDLPVPDSIAPGSADAAALQAE
                     MQQALDGLQPPACAYFGVRGENAVTSVNILTGKAAAIGFHIGDAPSLPLGLPRRVTMD
                     GRTSLIGAFLEVRAQSVGNQPAQRAYLWKVDPETGGARLIDDHGGDLERENHYVDDWL
                     VDSDGSLAARSVYDFRNRAFRIEMREQGRWKTVLSRTIVAEANTFAPYLIGVSGDGGS
                     IVILDTQAHGADGKGAERRFHYYGLKPSGELSGPLETGEASQARPVFNPRSGRLAGFA
                     YTAEEPRYDISDPGLRDLYDKAVSSTSGQTAEVLSVSDDERKALIRVTGGEDTGSYYR
                     LDYQNPQVDIIGEDYPLIPTPWIASQEMLSYKAQDGTGIDAVLTLPPEPADRNLPLVV
                     LPHDGPQGQDRIGFDWLAQALASRGYLVL"
     gene            complement(1693..>2543)
                     /locus_tag="VF402_00430"
     CDS             complement(1693..>2543)
                     /locus_tag="VF402_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEX7798780.1"
                     /translation="ILGNRAVIAVDQDPAGNQAVLAYDTDDVQILVKTLSDGHKAVAI
                     FNRGLAPEDVTLTARHLKFADNAPVMLTDLWSGAALAAFTGQIRLHVDPRQTLIYDAS
                     GARALSDGLYLSEMTGRVNPAVDGVVRPQPDPTIHRMINPWGATTDTGEPPAYAGWGG
                     AQADATPYRQALRIDGRMYDTGLGVLANSRFEVRADGAFRRFVAEVGVDDSTFDTDDA
                     VTFSVYGDGKLLATSKPARFGQPAQTLRADVTGVKIVELVARGGAAKDKLPTVVTWGK
                     AALLSD"
BASE COUNT          393 a          863 c          881 g          406 t
ORIGIN      
        1 agcaccagat agccgcgcga cgccagcgcc tgcgccagcc agtcgaagcc gatccggtcc
       61 tgcccctgcg gaccgtcgtg cggcagcacg accagcggca ggttcctgtc cgccggttcg
      121 ggcggcaagg tcaggaccgc atcgatgccg gttccgtcct gcgccttata ggacagcatc
      181 tcctgcgacg cgatccacgg cgtcgggatc agcggatagt cctcgccgat gatgtcgacc
      241 tgagggttct gatagtcgag ccggtaatag gagccggtgt cctcgccgcc cgtgacgcga
      301 atcagcgcct tgcgctcatc gtcggacacg gacagaacct ccgcggtctg gcctgaggtc
      361 gacgatacgg ccttgtcgta aagatcgcgc agccccggat cggagatgtc gtagcgcggc
      421 tcctccgccg tgtaggcgaa gcccgccaga cgcccggatc ggggattgaa aacgggccgc
      481 gcctgactgg cctcgcccgt ctccagcgga ccggaaagtt cgcccgacgg cttcaggccg
      541 taataatgaa agcgccgctc cgcccccttg ccgtccgcgc cgtgcgcctg cgtatccagt
      601 atgacgatgg agccgccatc gcccgacacg ccgatcaggt agggcgcgaa ggtgttggct
      661 tccgcgacga tggtgcgcga caggacggtc ttccaccgcc cctgttcgcg catctcgatg
      721 cggaaggcgc ggttgcggaa atcatagacc gagcgcgccg cgaggctgcc gtcggaatcg
      781 accagccagt cgtcgacata atggttttcg cgttcgagat cgccgccgtg atcgtcgatc
      841 aggcgggcgc cgccggtttc gggatcgacc ttccacagat aggcgcgctg ggcgggctga
      901 ttgccgaccg actgcgcgcg cacctccagg aaagcgccga tcaggctcgt cctgccgtcc
      961 atcgtcacgc gccgcggcag gccgagcggc aggctgggcg cgtcgccgat atgaaagccg
     1021 atcgccgccg ccttgccggt gaggatgttg accgaggtga cggcgttctc gccgcgcacg
     1081 ccgaaatagg cgcaggcggg cggttgcagc ccgtccagcg cctgctgcat ctcggcctga
     1141 agcgcggcgg cgtcggcgct gccgggcgcg atgctgtcgg gtacgggcag gtcgcgcgca
     1201 ttcggcttcg cgcccggccc cgacggcgcg ttctggcagg ttccgcgcag ggccagccgc
     1261 gagtccgaga tcagcacgtg cgtgttgtcg gcccagctca gcgcgctggc gggaccgccc
     1321 tcgacgcgga aaatctggtg gctcttgtcg gaaaaccggt aggcggccag cacgcgcgtg
     1381 tcgccgatgc gcgtcaggaa ggccacccgg tcgccgtcgg gcgacagcgc gacctcctcc
     1441 agacgcggca tgttcgcata aagctcaagc ggcggcggcg cgtcgaccgg cttgacgcgc
     1501 ttttcgcccc ggaccaccac ctcggtcggt ttttgggact gcgcgaaggc cgggccggac
     1561 agaagcgtca atgcggcgga agccagcagg atcgccttga agatgcgcat gggtttcccc
     1621 ccgatgacat aatggcgaac gccgctgcga tcgccgttat ggagtggcat ggaccggccg
     1681 gcgccgtcaa tctcagtcgc tcagcaaggc ggccttcccc caggtgacga cggtgggaag
     1741 cttgtccttc gccgccccgc cgcgcgcgac cagttcgacg attttcacgc ccgtgacatc
     1801 ggcgcgcagc gtctgggccg gctggccgaa acgcgccggt ttcgaggtcg ccagcagctt
     1861 gccgtcgcca tagacggaaa aggtcacggc gtcgtccgtg tcgaaggtcg aatcgtcgac
     1921 cccgacctcg gcgacgaaac gccggaaggc gccgtcggcc cgcacctcga agcgcgaatt
     1981 ggcgagcacg cccagccctg tgtcgtacat cctgccgtcg atgcgcagcg cctgacggta
     2041 gggggtggcg tcggcctgcg ccccgcccca gcccgcatag gcgggcggct cgccggtgtc
     2101 ggtcgtggcg ccccacgggt tgatcatgcg gtggatcgtc gggtccggct gcggacggac
     2161 gacgccgtcg accgccggat tgacgcgccc ggtcatctcg gacagataga ggccgtcgct
     2221 cagcgcgcgc gcgccgctgg cgtcgtagat cagcgtctgg cgcggatcga cgtgcagcct
     2281 gatctggccg gtaaaggccg caagcgcggc gccgctccac aggtccgtga gcatcaccgg
     2341 cgcgttgtcg gcgaatttca ggtggcgcgc ggtcagggtc acgtcctcgg gcgccaggcc
     2401 ccggttgaag atcgccaccg ccttatggcc gtcgctcagg gtcttgacca gaatctgcac
     2461 gtcgtcggtg tcgtaggcca gcacggcctg attgcccgcc ggatcctgat cgacggcgat
     2521 gacggcgcga ttaccgagaa tgt
//
LOCUS       DASVLL010000026         4733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_323652,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000026 DASVLL010000000
VERSION     DASVLL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323652"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2289)
                     /locus_tag="VF402_00435"
     CDS             complement(<1..2289)
                     /locus_tag="VF402_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7798781.1"
                     /translation="MSDTLNTTRRAFAAGAGISTLALTLGAASGAAAKAKKADAPLYK
                     DAGQPIEARIDDLMGRMTLEEKVMQMQCVWQAKGAIMDDKGEFDATKAQAVYPNGLGM
                     MARPSDRVGVHAVTGAGDNGATPNRNAFETATYINAVQKWAVEQTRLGIPLIMHEESL
                     HGYVARDATSFPQAIGLASSFDPDMAQKIFAVAAREMRARGANFALAPVVDVAREPRW
                     GRIEETYGEDPHLCGEMGKAAVLGFQGDSLPLAKDKVFATLKHMTGHGQPENGTNVGP
                     ADLGERELREDFFPPFEKIIHETNIRAVMPSYNEIDGVPSHANKWLLTTILRGEWGFK
                     GVTVSDYFAINELITRHHLVPDLTEAAFRAIKAGVDIETPDSAAYPHLIDLVKAGRVS
                     EDEINAPVRRILELKFQSGLFENPYVDAKLADKLTAAPDAVALAREAATRTVVLLKNN
                     GILPFQPKKLGKLLVLGTHARDTPIGGYSDVPRHVVSILDGVTAEGKAQGFEVAYSEA
                     VRITEGHVWGQDEVHFTPDSVNDKLIADALEAAKSADTILMVLGGNEQTSREAWADNH
                     MGDRQSLDLMGRQNELAEKIFALGKPTVVFLLNGRPLSVNLLNEKADALVEGWYMGQE
                     TGWAAADILFGRANPGGKLPVSIAHNVGQLPIYYNYKPTARRGYLDGTTAPLFPFGYG
                     LSYTTFDISAPRLAKPNMGVADSTEVSIDVKNTGAVAGDEVVQLYIRDDVSSVTRPVK
                     ELKHFKRITLQPGEQQTVTFTIS"
     gene            2466..3605
                     /locus_tag="VF402_00440"
     CDS             2466..3605
                     /locus_tag="VF402_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX7798782.1"
                     /translation="MRANLAPANINRRQSLLLGGAALAAAALPAGQTFAAPFEGPSLG
                     DLAAQKGMRFGTAIAAGPTGLRDPQVTAMVEAECNILVPENELKMYVTHNDNPVDYNF
                     APGDAILAFAESHKMAMRGHNLFWARDEFTPKWLKDYKFSGKAEAEKMLRDYIATVTE
                     HYGTRLTSWDVVNETVDPKTGEVRDNAFSRVLGMDALRISFEAAREHLPHMQLVYNDY
                     MSWEAGNEVHRAGVLKLMRWFRDNHVPVDALGVQSHIGNGFDLLGAQVNAWKDFLDEV
                     TGMGYDLLVTEFDVDDQTIPTDDIDKRDAIVATVGRLYLDQILSYKQTKDVLCWGMDD
                     KYSWLQGFTPRKDGARLRCTPYDDAFKPKPLREAIAAAFKTAPAR"
     gene            3771..4310
                     /locus_tag="VF402_00445"
     CDS             3771..4310
                     /locus_tag="VF402_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798783.1"
                     /translation="MFKRCLIAAAAVAMLAPLAARAADDPMQHLINNPNPASLIIYGP
                     QTNKKIKDASVQGGAAIEVKAQGVGESYAAAAQTSLDKPIKAGDQIECDVFLKATRDD
                     GQAPTLHARVQVNEAPYSGVGAETDFNVTNQWALYTLKTTADKDYDKGKLVFVVHLNS
                     ARQTVDIGPVFVFDNSRTF"
     gene            4471..>4733
                     /locus_tag="VF402_00450"
     CDS             4471..>4733
                     /locus_tag="VF402_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7798784.1"
                     /translation="MPVIDLSPQAVAEALQAGEILLVDVREPYEFDAERIKGSISLPL
                     SAIDPAAVPQAEGKTVVLSCAGGVRSARAAEYLQATGLDVKHHL"
BASE COUNT          817 a         1549 c         1529 g          838 t
ORIGIN      
        1 cgagatggtg aaggtcacgg tctgctgttc gcccggttgc agggtgatgc gcttgaaatg
       61 cttcagttcc ttgaccgggc gcgtgaccga ggacacgtcg tcgcggatat agagctgcac
      121 cacttcgtcg cccgccacgg cgccggtgtt cttcacgtcg atgctgacct cggtcgaatc
      181 ggcgacgccc atgttcggct tggccaggcg cggcgcggag atgtcgaacg tggtatagga
      241 aaggccgtac ccgaacggga acagcggcgc ggtcgtgccg tccagataac cccggcgcgc
      301 ggtcggcttg taattgtagt agatcggcaa ctgcccgaca ttgtgcgcga tcgacaccgg
      361 cagcttgccg cccggattgg cgcggccgaa caggatgtcg gcggcggccc agccggtttc
      421 ctgccccatg taccagcctt cgaccagcgc gtcggccttt tcgttgagca ggttgaccga
      481 tagcgggcgt ccgttgagca aaaagaccac ggtgggcttg cccagcgcga agatcttttc
      541 cgccagctcg ttctgacggc ccatcaggtc cagcgattgc cggtcgccca tgtggttgtc
      601 ggcccaggct tcgcgcgagg tctgctcgtt gccgcccagc accatcagga tggtgtccgc
      661 cgacttcgcc gcctccagcg cgtccgcgat cagcttgtca ttgaccgaat ccggcgtgaa
      721 gtgcacctcg tcctggcccc agacgtgacc ttcggtgatg cgcaccgctt cggaataggc
      781 gacttcgaag ccctgcgcct tgccttccgc cgtcacgccg tcgaggatcg agacgacatg
      841 gcgcggcacg tcggaatagc cgccgatcgg cgtgtcgcgc gcatgcgtgc ccagcaccag
      901 cagcttgccc agctttttcg gctggaacgg caggatgccg ttgttcttga gcagcaccac
      961 cgtcctggtc gccgcctcgc gcgccagcgc cacggcgtcc ggcgccgcgg tcagcttgtc
     1021 ggccagtttg gcgtcgacat aggggttttc aaacaggccg gactggaatt tcagctccag
     1081 gatgcggcgc accggcgcgt tgatctcgtc ctcggaaacg cggcccgcct tgaccagatc
     1141 gatcaggtgc ggataggcgg cgctgtccgg ggtttcgatg tcgacgcccg ccttgatggc
     1201 gcggaaggcg gcctcggtca ggtcgggcac gaggtggtgg cgcgtgatca gttcgttgat
     1261 ggcgaaatag tcggacaccg tgacgccctt gaagccccat tcgccgcgca ggatggtggt
     1321 cagcagccac ttgttggcgt gcgacggcac gccgtcgatc tcgttatagg acggcatgac
     1381 cgcgcggata ttggtctcgt ggatgatctt ctcgaacggc gggaagaagt cctcgcgcag
     1441 ttcgcgctcg cccaggtccg ccgggccgac attggtgccg ttttccggct ggccgtggcc
     1501 ggtcatgtgc ttgagcgtgg cgaacacctt gtccttggcc agcggcaggc tgtcgccctg
     1561 gaagcccagc acggcggcct tgcccatctc gccgcacagg tgcgggtctt cgccgtaggt
     1621 ttcctcgatg cggccccagc gcggctcgcg cgccacgtcg accaccggcg ccagcgcgaa
     1681 attggcgccg cgcgcgcgca tctcgcgcgc cgccaccgca aatatcttct gcgccatgtc
     1741 gggatcgaag gacgaggcca gaccgatggc ctgcgggaag gaggtcgcgt cgcgcgcgac
     1801 atagccgtgc aagctttcct cgtgcatgat cagcgggatg ccgaggcggg tctgctcgac
     1861 cgcccacttc tgcacggcgt tgatataggt cgccgtctcg aaggcgttgc gattcggcgt
     1921 cgcgccgtta tcgcccgccc cggtgaccgc atggacgccg acgcggtccg acgggcgcgc
     1981 catcatgccg aggccgttcg gatagacggc ctgcgccttc gtggcgtcga actcgccctt
     2041 gtcgtccatg atcgcgccct tggcctgcca gacgcattgc atctgcatga ccttttcctc
     2101 cagcgtcatg cggcccatca ggtcgtcgat gcgcgcctcg atcggctggc ccgcgtcctt
     2161 gtacagcggc gcatcggcct ttttcgcctt ggcggcggcg cccgaggcgg cgcccagcgt
     2221 cagggccagg gtggaaatgc ccgcgccggc ggcgaaggcg cggcgggtcg tgttgagggt
     2281 gtcgctcata atatcttccc tgtgatgtcg ccgatgcagg ttcggctgtt gttgcgttcc
     2341 gggcgcggct tgtcagaccc atcctaccgt tttcggtccg cgtcaagtcg cggcggagat
     2401 tgacgaagcg cgcgattgct gtcactgtta gcggtaacag aaaaaagttc aacagggaat
     2461 tttccatgcg cgcaaatctc gcacctgcga atatcaatcg ccgtcaatcg cttttgctgg
     2521 gcggggcggc gctggcggcg gcggccctgc cggctggtca gacctttgcg gcgcctttcg
     2581 aaggcccgtc cctgggcgat ctggcggcgc aaaaaggcat gcgtttcggc acggcgatcg
     2641 cggccggccc gaccggcctg cgcgatccgc aggtcacggc gatggtggag gcggaatgca
     2701 acatcctcgt gcccgagaac gagctgaaga tgtacgtcac gcacaacgac aatccggtgg
     2761 attacaattt cgcgcccggc gacgcgattc tggccttcgc cgaatcgcac aagatggcga
     2821 tgcgcggcca caacctgttc tgggcgcgcg acgagttcac gccgaaatgg ctgaaggatt
     2881 acaaattctc cggcaaggcc gaggccgaaa agatgctgcg cgactatatc gccacggtca
     2941 cggagcatta cgggacgcgc ctgacctcat gggacgtggt caacgaaacc gtcgatccca
     3001 agaccggcga ggtgcgcgac aacgcctttt cgcgcgtgct gggcatggac gccttgcgca
     3061 tcagcttcga ggcggcgcgc gaacatctgc cccatatgca actggtttat aacgactata
     3121 tgagctggga ggccggcaac gaagtccacc gcgcgggcgt gctgaaactg atgcgctggt
     3181 tccgcgacaa ccatgtgccg gtcgacgcgc tgggcgtgca gagccatatc ggcaacggct
     3241 tcgaccttct gggcgcgcag gtcaacgcct ggaaggactt tctcgacgag gtcaccggca
     3301 tgggctacga cctgctggtc acggagttcg acgtcgacga ccagaccatc cccaccgacg
     3361 atatcgacaa gcgcgacgcc atcgtcgcca cggtcgggcg gctctatctc gaccagatcc
     3421 tcagctacaa gcagacgaag gacgtgttgt gctggggcat ggacgacaag tatagctggc
     3481 tgcaaggctt cacgccgcgc aaggacggcg cgcgcctgcg ctgcacgccg tatgacgacg
     3541 ccttcaagcc caagcccctg cgcgaggcga tcgccgcggc gttcaaaacc gccccggcgc
     3601 ggtaagccag aggctggcca aaactgacac atgccaaact gacacatgaa tgcaacggtg
     3661 gagcgcaagt tccaccgtgc gtctgcgtcg gtgtggccag tttcgatttt gtgtggcagc
     3721 ctgccctgaa aacaggacgg ataatcgtcc gacagtccag ggagatcacc atgttcaagc
     3781 gctgccttat cgccgccgcc gccgtcgcca tgctcgcgcc gctggcggcg cgcgccgccg
     3841 acgatccgat gcaacatctg atcaacaacc ccaatccggc cagcctgatc atctacggtc
     3901 cgcagaccaa caagaagatc aaggacgcca gcgtgcaggg cggcgccgcc atcgaggtca
     3961 aggcgcaggg cgtcggggaa tcctacgccg ccgccgccca gaccagtctc gacaagccga
     4021 tcaaggccgg cgatcagatc gaatgcgacg tcttcctgaa agcgacccgc gacgacggcc
     4081 aggcccccac cctgcacgcg cgcgttcagg tcaacgaggc cccctattcg ggcgtcggcg
     4141 ccgaaaccga cttcaacgtc accaaccaat gggcgctgta cacgctgaaa accaccgccg
     4201 acaaggatta cgacaagggc aagctggtct tcgtcgtcca cctgaacagc gccaggcaga
     4261 ccgtcgacat cggcccggtc ttcgtcttcg acaacagccg gacgttctga atcgggctga
     4321 accgggctga accgggcctc tccccgtccc gatcggcatg accccgctcg tccgggcgtg
     4381 gacctccacg cctgatatga gcggacaagg cttgccgcgc cacgcggaaa ggcgtacggg
     4441 cggggaacgc tttttcagga gcctgcgccc atgcccgtga tcgatctgtc gccgcaagcc
     4501 gtcgccgaag cgctgcaagc gggcgaaatt ctgctcgtcg acgtgcgcga accgtatgag
     4561 ttcgacgccg agcgcatcaa gggttcgatc agcctgccgc tgtcggccat cgatcccgcc
     4621 gccgtgccgc aggccgaggg caagaccgtc gtgctgtcgt gcgcgggcgg ggtgcgctcg
     4681 gcgcgcgcgg cggaatatct gcaagccacc ggcctcgacg tgaagcacca tct
//
LOCUS       DASVLL010000027         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_484702,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000027 DASVLL010000000
VERSION     DASVLL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484702"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VF402_00455"
     CDS             complement(<1..202)
                     /locus_tag="VF402_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7798785.1"
                     /translation="MTPSDVFCEAVAGWLGERTPLVVGVCAAQGAGKSTLCRTAKAAG
                     IVVTNTPDVLTEATADIALMLML"
     gene            complement(189..1154)
                     /locus_tag="VF402_00460"
     CDS             complement(189..1154)
                     /locus_tag="VF402_00460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7798786.1"
                     /translation="MAKPKILLTRPWPEGAQGWLQDRFDVTVHPGPGLDAAGLVQALR
                     DYDALGVAVNDKMTQEVFAAPGPRARLVANFGVGFEHIDVAAAKAAGIVVTNTPDVLT
                     EATADIALMLMLMASRRAGEGERQVRAGHWKGWGAKALLGRSLQGKTLGLVGFGRIAQ
                     ATARKAHGALDMRVAYYGRRRAAPEIEAETGARYVSSLDDLAAEADVLSLHVPGGGDT
                     HHLIDAVRLERMKPDAILINTARGPVVDEAALAQALRDGRIWAAGLDVYEHEPKVDPD
                     LLALDNVVLLPHLGSATVETREAMGMRATENIALFFNGQPVRDAL"
     gene            complement(1210..2253)
                     /locus_tag="VF402_00465"
     CDS             complement(1210..2253)
                     /locus_tag="VF402_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7798787.1"
                     /translation="MPNFTSGRPPTIYDVAALSGVGRTTVSRVLNGDAHVAGPTRERV
                     LEAVRKLRYRVNPQARLLAGRRPHALMVIYPVNDEVGPNTWYNLLVESGALRACGKAG
                     LQLLMQQVFPHSLSKHARVMELIDNHACDGMILAPPFSDDAELIAMIRARQMPLACLA
                     AGPATRGLAPGVGIDDEAAGYDLTRFLLAQGHRRFAFIDGLREHLSASLRYAGFRRAL
                     EGAGLDPDAARTARGNLTFLGGQEQLEGLLDGRPTAVVCANDDMAAGALYRAHQLGLS
                     VPRDLSVAGFDDAPFTQMIWPPLTTISQPVFAMAQRAVDIVLHVISDPHARADYELVP
                     HRIVKRDSVAAPV"
     gene            complement(2472..>3169)
                     /locus_tag="VF402_00470"
     CDS             complement(2472..>3169)
                     /locus_tag="VF402_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7798788.1"
                     /translation="GRGNGGAAAGRAGPADRAHGQDVVILHADAHLLIAVKPAGLLSV
                     PGRGADKQDCLIARLQADYPDALTVHRLDMATSGVMAFGRGIEAQRALSAAFAERRVA
                     KSYVAVVAGHVAEAAGEVDLPLICDWPNRPLQKVDHERGKRALTRWRVLARGIVGGGG
                     VTRVELEPVTGRSHQLRVHMAQMGHPILGDPFYAPPAVRAMSARLLLHARALPVPHPA
                     DAEMTAFAAEPGF"
BASE COUNT          475 a         1187 c         1043 g          464 t
ORIGIN      
        1 tcagcatcag catcagcgcg atgtccgccg tcgcctcggt cagcacgtcc ggcgtgttgg
       61 tcaccacgat ccccgccgcc ttcgccgtcc ggcacagggt cgacttgccc gccccttgcg
      121 ccgcgcagac gccgacgacc agcggcgtcc gttcgcccag ccatccggcg acggcctcgc
      181 aaaagacgtc agagggcgtc acggaccggc tgcccgttga aaaacagcgc gatattctcc
      241 gtcgcgcgca ttcccatcgc ctcgcgcgtc tcgacggtgg cgctgccgag atgcggcagc
      301 agcacgacat tgtcgagcgc cagcaggtcg ggatcgacct tcggctcatg ctcgtaaacg
      361 tccagacccg ccgcccagat gcgcccgtcg cgcaaggcct gcgccagcgc cgcctcgtcc
      421 accaccggcc cgcgcgcggt gttgatcagg atcgcgtccg gcttcatgcg ttccagccgc
      481 accgcgtcga tcaggtgatg cgtatccccg ccgcccggca cgtgcagcga caaaacgtcc
      541 gcctccgccg ccagatcgtc gagcgaggac acgtagcgcg cgcccgtctc ggcctcgatc
      601 tccggcgcgg cgcggcgacg gccgtaatag gcgacgcgca tgtccagcgc gccgtgcgcc
      661 ttgcgcgccg tcgcctgcgc gatgcgcccg aagccgacca gccccagcgt cttgccttgc
      721 agcgaccgcc ccagcagcgc cttggcgccc cagcccttcc aatgcccggc gcgcacctgc
      781 cgctcgccct cgcccgcccg gcgcgacgcc atcagcatca gcatcagcgc gatgtccgcc
      841 gtcgcctcgg tcagcacgtc cggcgtgttg gtcaccacga tccccgccgc cttcgccgcc
      901 gccacgtcga tatgctcgaa cccgacgccg aaattggcga ccagccgcgc gcgcggcccc
      961 ggcgcggcga acacctcctg cgtcatcttg tcgttcaccg ccacgcccag cgcgtcatag
     1021 tcccgcagcg cctgaaccag ccccgccgcg tccagccccg gccccggatg caccgtgacg
     1081 tcgaagcggt cctgaagcca gccttgcgcg ccttcgggcc agggacgggt gagcagaatt
     1141 ttcggtttcg ccataaggag cctccaatcg cctgatgttt tggacgacag gcggcgggga
     1201 cttcaagcgc taaaccggcg ccgcgaccga atcccgcttc acgatgcggt gcgggaccag
     1261 ttcgtaatcg gcgcgcgcgt gcggatcgga gatgacgtgc agcacgatgt cgaccgcgcg
     1321 ctgggccatg gcgaacaccg gctgcgagat ggtggtcagc ggcggccaga tcatctgggt
     1381 gaacggcgcg tcgtcgaaac cggcgacgct gaggtcgcgc ggcacgctca ggcccaactg
     1441 gtgcgcacgg tacaaggccc ccgccgccat gtcgtcattg gcgcagacca ccgccgtcgg
     1501 gcggccgtcc agcaggccct caagctgctc ctgcccgccg aggaaggtca ggttgccgcg
     1561 cgccgtgcgc gctgcgtcgg ggtcgaggcc cgccccttcc agcgcgcggc ggaacccggc
     1621 gtagcgcagg ctggccgaca ggtgttcgcg cagaccgtcg atgaaggcga agcgccggtg
     1681 cccctgcgcc agcaggaagc gcgtcaggtc gtagcccgcc gcctcgtcgt cgatgccgac
     1741 gccgggcgcc aggccgcgcg tcgccggtcc cgccgccagg caggccagcg gcatctgccg
     1801 cgcacggatc atcgcgatca gttcggcgtc gtccgagaac ggcggcgcca ggatcatgcc
     1861 gtcgcaggca tggttgtcga tcagctccat cacccgcgcg tgcttgctca gcgaatgcgg
     1921 gaacacctgc tgcatcaaaa gttgcaagcc cgccttgccg caggcgcgca gcgcgccgct
     1981 ttcgaccagc aggttgtacc aggtgttcgg cccgacctcg tcattgaccg gatagatgac
     2041 catcagcgcg tgcgggcgcc tgcccgccag cagccgcgcc tgcggattga cgcggtagcg
     2101 cagcttgcgc accgcctcca gcacgcgctc gcgcgtcggg ccggcgacgt gcgcgtcgcc
     2161 gttgaggacg cgcgaaaccg tggtgcggcc gacgccggac aaggcggcga catcgtagat
     2221 ggtcggggga cggccggacg tgaaattggg catgtgacgt ttcgaacctg tggcgagttc
     2281 tgtggcgccg tttttcagtt tttggcgacc ttatatgtcc ccatagtacg cgacggcggc
     2341 ggcgcgtcaa tcgtcctcct gtcgccgatt tgccacaggc cgcctgagcg cgcgaaggca
     2401 atgccctttg ctttcagaaa cttaccgcgc ccgcgaaaac ttccgccctc tgtgggagcg
     2461 agaccatccg gctaaaaccc aggctccgcc gcgaaggccg tcatctccgc gtccgccgga
     2521 tgcggcacgg gcagcgcgcg cgcgtgcagc agcagccgcg cgctcatcgc ccgcacggcg
     2581 ggcggcgcat agaacggatc gccgaggatc ggatggccca tctgcgccat atggacgcgc
     2641 aactgatggg accggccggt gacgggctcc agttcgacgc gcgtgacgcc cccgccgccc
     2701 acgatcccgc gcgccagcac gcgccagcgc gtcagggcgc gcttgccgcg ttcatgatcg
     2761 accttttgca gcgggcggtt cggccagtcg cagatcagcg gcagatcgac ctcgcccgcc
     2821 gcctccgcca cgtgtccggc cacgacggcg acatagctct tcgcgacgcg gcgttcggcg
     2881 aaggcggcgg acagcgcccg ctgcgcctcg atcccgcgcc cgaacgccat cacgcccgag
     2941 gtcgccatgt cgagccggtg gacggtgagc gcgtcgggat agtcggcctg aaggcgggcg
     3001 atcaggcaat cctgcttgtc cgcgccgcgc cccggcacgc tgagcagccc cgccggtttg
     3061 accgcgatca gcaggtgcgc gtcggcgtgc aggatcacaa cgtcttgccc atgcgcacga
     3121 tcggcaggtc cagcccgccc ggcagcggcg ccgccgtttc ctcgaccgg
//
LOCUS       DASVLL010000028         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_216386,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000028 DASVLL010000000
VERSION     DASVLL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216386"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1704)
                     /locus_tag="VF402_00475"
     CDS             complement(<1..>1704)
                     /locus_tag="VF402_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX7798789.1"
                     /translation="LVVEERRALIEPQLRDALYDLPGRRPRVLGKRDASGAPLLRDLM
                     EFDSAHVALALFRVLPEAAKTPERRALAAKLEQAACARDGLHPLHVRSPFYCAGCPHN
                     SSTTVPEGGRALAGIGCHYMAQFMPDRRTDLVTQMGGEGVTWVGQAPFTDEKHVFVNL
                     GDGTYFHSGVLAIRQAVAANVNITYKILYNDAVAMTGGQHVDGQLSPITVAQQVATEG
                     VARIILVSETPERWRNHPKMPARVSFYHRDAFAAAEAELQATKGVTVLIYDQMCATER
                     RRRRKRGLAPQATQRIFINAAVCEGCGDCSAKSNCVAVGPKETELGRKREIDQSACNI
                     DTSCVKGFCPSFVALDGAAPKKAGPAAIGHLIADLPEARPATMTDQPFNVLLTGIGGL
                     GVTSLSAILGMAAHIDGLEAVCVDQTGLAQRGGAVDAHVRLLKSGVVPGGRIPLGETD
                     VLIAADMVTAHGKSCLPLLDKARTHGFLDARLTPTAEFTLDTKTVFDPAAMTRRVRAA
                     VRRLDGFDAGGLARSYLGDAVFSLMVMLGAAWQSGALPLTRAAVEKAIALNGAAVKAN
                     L"
BASE COUNT          264 a          602 c          575 g          263 t
ORIGIN      
        1 cgagattggc cttcaccgcc gcgccgttca acgcgatcgc cttttcgacc gccgcgcgcg
       61 tcagcggcag ggcgcccgac tgccaggccg cgcccagcat caccatcagc gaaaacaccg
      121 cgtcgccgag atagctccgc gccagaccgc cggcgtcgaa gccgtcgagc cgccgcaccg
      181 ccgcgcgcac gcgccgcgtc atcgccgccg gatcgaacac ggtcttcgtg tcgagcgtga
      241 attccgccgt cggcgtcagg cgcgcgtcga ggaagccgtg ggtgcgcgcc ttatcgagca
      301 gcggcaggca cgatttgcca tgcgccgtca ccatgtcggc ggcgatcagc acgtccgtct
      361 cgcccagcgg aatgcgcccg ccgggaacca cgcccgattt cagcaaacgc acatgcgcat
      421 cgaccgcgcc gccgcgctgc gcgaggccgg tctgatcgac gcacaccgcc tccagcccgt
      481 cgatatgggc cgccatgccg agaatggcgc tcaaggacgt gacgcccagc ccgccgatgc
      541 cggtcagaag cacgttgaac ggctgatccg tcatcgtcgc gggccgggct tcgggcaggt
      601 ccgcgatcag gtggccgatg gcggcgggtc cggccttttt cggcgcggcg ccgtccagcg
      661 ccacgaagct ggggcagaag cccttgacgc acgatgtgtc gatattgcac gccgactgat
      721 cgatctcgcg cttgcggccc agctcggttt ccttcgggcc gaccgcgacg cagttggatt
      781 tggccgaaca gtcgccgcag ccttcgcata ccgccgcgtt gatgaagatg cgctgcgtgg
      841 cctgcggcgc cagaccgcgc ttgcggcggc ggcggcgctc ggtcgcgcac atctgatcgt
      901 agatcagcac ggtgacgccc ttcgtcgcct gcaattcggc ctcggcggcg gcgaaggcgt
      961 cgcggtgata aaagctgacg cgcgcgggca ttttcggatg gttgcgccag cgctccggcg
     1021 tttcggacac caggatgatg cgcgccacgc cttcggtggc cacctgctgc gccaccgtga
     1081 tcggactgag ttggccgtcg acatgctggc cgccggtcat ggcgaccgcg tcattataga
     1141 ggatcttgta ggtgatgttg acgttcgccg cgaccgcctg acggatggcc agcacgccgg
     1201 aatggaaata ggtgccgtcg cccaagttga cgaagacgtg cttttcgtcg gtgaacggcg
     1261 cctgaccgac ccaggtgacg ccctcgccgc ccatctgggt gacgaggtcg gtgcgccggt
     1321 ccggcatgaa ctgcgccatg tagtgacagc cgatcccggc cagcgcgcgc ccgccttccg
     1381 gcacggtcgt ggagctattg tgcgggcatc ccgcgcaata aaaaggcgag cgcacatgca
     1441 gcggatgcag gccgtctctg gcgcaggcgg cctgctccag cttcgcggcc agcgcacggc
     1501 gctccggcgt cttcgccgcc tccggcaaca cgcggaacag ggccagcgcc acatgcgccg
     1561 agtcgaactc catcaggtcg cgcagcaagg gcgcgccgga cgcgtccctt ttgcccagca
     1621 cccgcgggcg gcggcccggc aggtcataga gggcgtcgcg cagttgcggc tcgatcaggg
     1681 cgcggcgctc ctcgaccacc agca
//
LOCUS       DASVLL010000029         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_431452,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000029 DASVLL010000000
VERSION     DASVLL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431452"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VF402_00480"
     CDS             complement(<1..656)
                     /locus_tag="VF402_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX7798790.1"
                     /translation="MVEVVERDVDGELWVKWGKPDALKARSVSATENQTPLARLAPLK
                     KNVAQTPPGMGDRLYVRFEKIADGWVAHLIKILDQGAPRIVGVVRRSKRELRLESVNR
                     KEKNSFSLTGAGLEALEDGDVVVAAPTGGAHRYGPRPARVVEVIGKEDSPRIASVMAI
                     HSHGLPIGFAAATEKEAESARAPGLGKRTDLRDLPFVTIDPVDAKDHDDAVYAHADDD
                     A"
     gene            complement(962..1342)
                     /locus_tag="VF402_00485"
     CDS             complement(962..1342)
                     /locus_tag="VF402_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798791.1"
                     /translation="MRLFIALAASLALAAGGAAAVHAAPAQSKMQACAAQWNQLKAAN
                     KTGGQTYKTFSSACMKGNAATPAVKPAPAKVTPAKVAATKVAASKPQDRMKQCAAQWN
                     QLKAANKTGGQTYKQFSASCLKKA"
     gene            complement(1448..>2663)
                     /locus_tag="VF402_00490"
     CDS             complement(1448..>2663)
                     /locus_tag="VF402_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEX7798792.1"
                     /translation="KKMEELGIGRPSTYASILGVLRDREYVNMDKNRFVPSDKGRLVT
                     AFLEEFFRRYVEYDFTADLEEKLDAVSAGKLDWKALLREFWNQFHASVGEIAELRTGQ
                     VLEALNEALGPHIFPDKGDGSNPRACPACANGQLSLKTSRFGAFIGCSNYPECSYTRP
                     VGAPGSEAENAVSGDKDLGVDPETGLTVALKVGRFGPYVQLGEAASKEDKPRRSSLPK
                     AWPPASIDLDRALKLLSLPRAIGVHPEDQKPIVAGLGRFGPFVESGGTYANLSNFDEI
                     FEVGINRAVDLIAQKRAGGKGGRGAAAAPLRELGVYPNSDEKIAVMSGRYGPYVKAGK
                     TNATLPKEVTPETVTLEQAIALIDAKGGLKAAGAKKAPAKKAAAKKAPAKKTPAKKTA
                     RAAPRKTADGPA"
BASE COUNT          353 a          887 c          931 g          492 t
ORIGIN      
        1 gcgtcgtcgt cggcatgggc gtaaacggcg tcgtcatggt ccttggcgtc caccgggtcg
       61 atggtcacga agggcaggtc gcgcaggtcc gtgcgtttgc cgaggccggg cgccctggcg
      121 ctttcggcct ccttctcggt ggcggcggcg aagcctatgg gcaggccgtg gctgtggatc
      181 gccatgaccg aggcgatgcg cgggctgtcc tccttgccga tcacctcgac gacgcgggcg
      241 ggcctggggc cgtagcggtg cgcgccgccg gtcggggccg cgaccacgac atcgccgtcc
      301 tccagcgcct caagaccggc gccggtcagc gaaaagctgt tcttttcctt gcggttgacc
      361 gattccagcc gcagttcgcg ctttgagcgc cgcaccacgc cgacgatacg cggcgcgccc
      421 tgatcgagga tcttgatcag gtgcgcgacc cagccgtcgg cgatcttctc gaagcgcaca
      481 tagagccggt cgcccatgcc gggcggcgtc tgggcgacgt tctttttcag cggcgcgagg
      541 cgggccagcg gcgtctggtt ttcagttgcg ctaacgcttc gcgccttgag cgcgtccggc
      601 ttgccccact tgacccacag ttcgccgtcg acgtcgcgct ccacgacctc gaccacgccg
      661 gtttccggca gggcgcccag cgcgccgaag ctcttgcgcc cgttgcgcgc cagcttgccg
      721 ctttcggcca gcgccttcag ccgctgccgc agggcgcggc ggtcgtcgcc cttgaggccg
      781 aagcgtttgg ccagatcgcc gatatccagc tcgcgttgat ccgcgacggc gtcgagcagc
      841 gccttgtcgt cgggcaggcc gggggacgcg ccttcagggc ggcggacggg ggccgcgcgg
      901 cggcatggaa aagcgccgga cgccgcgggc gtccggcgct taggggcgcg cgtgcgcggc
      961 ctcaggcttt cttcaggcag gaagcagaga actgcttata ggtctggccg ccggtcttgt
     1021 tcgcggcctt gagctggttc cactgggcgg cgcattgctt catgcggtcc tgcggcttgc
     1081 tggccgcgac ctttgtggcg gcgaccttgg cgggggtgac cttggcgggg gcgggcttga
     1141 ccgccggagt ggccgcattg cccttcatgc aggcggacga gaaggtcttg taagtctggc
     1201 cgccggtctt gtttgccgcc ttgagttgat tccactgggc ggcgcaggcc tgcatcttgc
     1261 tttgcgcggg ggcggcgtgg acggcggcgg cgccgccggc ggccagggca agcgaagcgg
     1321 cgagggcgat aaacagacgc atggggctaa ctcccggatt gtcaccggcg aaactgccgg
     1381 tatgtccgtg atgatccgca atgaaggcgg atttagcgcg tggattgttt cagtatcgac
     1441 acaaaactca ggccgggccg tccgccgttt tccggggcgc ggccctggcg gtctttttcg
     1501 caggcgtctt tttggcggga gccttcttcg ccgccgcttt cttcgcgggg gcctttttcg
     1561 caccggcggc tttcaacccg cccttggcgt caatcagcgc gatcgcctgc tccagcgtga
     1621 ccgtctccgg cgtcacctcc ttgggcaggg tggcgttggt cttgcccgcc ttgacatagg
     1681 ggccgtagcg cccgctcatc acggcgatct tctcgtccga gttcggatag acgcccagtt
     1741 cgcgcaaagg cgccgccgcc gcgccgcgtc cgcccttgcc gcccgcgcgc ttttgcgcga
     1801 tcaggtcgac ggcgcggtta atgcccacct cgaagatttc atcgaagttc gacaggttgg
     1861 cgtaggtgcc gcccgattcc acgaacggcc cgaagcggcc gaggcccgcg acgatcggct
     1921 tctgatcctc cgggtggacg ccgatcgcgc gcggcaggct gagcagtttc agcgcgcggt
     1981 cgaggtcgat gctggccggc ggccacgcct tcggcagcga cgagcgccgg ggcttgtcct
     2041 ccttcgaggc ggcttcgcca agctgcacat aggggccgaa gcgcccgacc ttgagcgcca
     2101 cggtgaggcc ggtttcggga tcgacgccga gatccttatc gcccgacacg gcgttctcgg
     2161 cttccgaacc cggcgcgccg acgggccgcg tatagctgca ttcgggatag ttggagcagc
     2221 cgataaacgc cccgaaacgc gaggttttca ggctcaactg accgttggcg caggccgggc
     2281 aggcgcgcgg attcgagccg tcgcccttgt cggggaagat gtgcggtccg agcgcctcgt
     2341 tcagcgcctc cagcacctgg ccggtgcgca gctcggcgat ctcgccgacc gaggcgtgga
     2401 actggttcca gaactcgcgc agcagcgcct tccagtccag cttgcccgcc gacaccgcgt
     2461 cgagcttctc ctccaggtcg gcggtgaagt cgtactccac gtagcggcgg aagaactcct
     2521 ccaggaaggc ggtgaccagc cggcccttgt cggaagggac gaaccggttc ttgtccatgt
     2581 tcacgtattc gcggtcgcgc agcacgccca ggatcgaggc gtaggtggag ggccggccga
     2641 tgcccagctc ctccatcttc ttg
//
LOCUS       DASVLL010000030         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_1648, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000030 DASVLL010000000
VERSION     DASVLL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..678
                     /locus_tag="VF402_00495"
     CDS             28..678
                     /locus_tag="VF402_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEX7798793.1"
                     /translation="MSDPVHPAETPAEKTAEKPASKLELRRQVQLEALLTAAEARVRR
                     DGVASLKARDLAADIGVALGGLYNIVADMDDLMLRVNSRTLARLGTAAAEAADGLPMT
                     TRAEALNRLATVARTYLGFARIDPRLWRMLFETHVASALPAWANEDQLRLFRHIAEPL
                     SVIMPDLPPDALTVRARTLFAAVHGVVTLGLEERLVAVPLDRLESEIDWLTRAACG"
     gene            complement(722..1819)
                     /locus_tag="VF402_00500"
     CDS             complement(722..1819)
                     /locus_tag="VF402_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX7798794.1"
                     /translation="MSRVQTSTLTDLIELAKEPSSAKRRELLRKVTDVFMTHPEQVGE
                     QEMALYDSVMSQLSADMESAVRAEIAGAIAGARTAPLGLLRKLASDEIAVAEPILTRS
                     RALSESDLVHIVSTQGQDHLRAVSRREEVPESVSGVIVKRGDDATLHTLLSNDGARLS
                     RAASEAVVQRAQVNPDLHEVVVNRADLPPDLMNDMYFVVESRLRERILAENAKLDPSV
                     IDSALSKGRNRVAVAYGAFPEDYERIWSEVETLRRNEKLTPSLLAKYMRDPNPTWFLV
                     ALAQMADIDYLTAKHVVDRREIDALAIACKAAGLDKALFLTCAMIMLSDSENAMGRAA
                     EYGKLYQDLPYDTAQRTIRFWRLRRAGGHAA"
     gene            complement(1831..>2138)
                     /locus_tag="VF402_00505"
     CDS             complement(1831..>2138)
                     /locus_tag="VF402_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798795.1"
                     /translation="TAAKPAATVPAPAPSLASQETDHVFIPGEHYTDASDAPRFYSLH
                     REYGYKPDPITVDDNATGALLTVAPDKSAKSDDDSTSDSDSTADSTPDANNGQKASP"
BASE COUNT          313 a          677 c          769 g          379 t
ORIGIN      
        1 tgttcaaaaa atatttgcga gtccgccatg tccgacccgg tccatcctgc cgaaacaccc
       61 gccgaaaaaa ccgccgaaaa acccgcaagc aagctggagc tgcgccgcca ggttcagctt
      121 gaggcgttgc tgaccgccgc cgaggcgcgc gtgcgccggg acggcgtcgc cagtctgaag
      181 gcgcgcgatc tggccgccga tatcggcgtc gcgctgggcg gcctctacaa tatcgtcgcg
      241 gacatggacg acctgatgct gcgggtgaat tcgcgcacgc tggcccggct gggcacggcg
      301 gcggccgagg cggcggacgg cctgcccatg acgacgcgcg ccgaggcgct gaaccggctg
      361 gcgacggtgg cgcggaccta tctcggcttc gcgcgcatcg acccgcggtt gtggcggatg
      421 ctgttcgaga cgcatgtcgc ctcggccctg ccggcctggg ccaatgagga ccagttgcgc
      481 ctgttccgcc atatcgccga gccgctgagc gtcatcatgc ccgacctgcc gccggacgcg
      541 ctcacggtgc gcgcgcgcac cctgttcgcc gccgtgcatg gcgtcgtgac tctggggctg
      601 gaggagcggc tggtggccgt gccgctggac aggctggaaa gcgagatcga ctggctgacc
      661 cgcgcggcgt gcgggtaaag atcaggcggc gcgccccccg gcgcggccca aattttacat
      721 cctatgcggc gtgccccccg gcgcgccgca ggcgccagaa gcggatcgtg cgttgcgcgg
      781 tgtcgtaagg cagatcctga tacagcttgc cgtattcggc ggcgcggccc atggcgtttt
      841 ccgaatccga cagcatgatc atcgcgcagg tcaggaacag cgccttgtcg aggcccgcgg
      901 ccttgcaggc gatggccagc gcgtcgattt cgcgacggtc gacgacgtgc ttggcggtca
      961 ggtagtcgat gtcggccatc tgggccagcg cgaccaaaaa ccaggtcggg ttcggatcgc
     1021 gcatgtattt ggccagcagc gacggcgtca gcttttcgtt gcggcgcagg gtttcgacct
     1081 cggaccagat gcgttcgtag tcctcgggga aggcgccgta ggcgacggcg acgcggttgc
     1141 ggcctttcga cagggcgctg tcgatcacgg atgggtccag tttggcgttc tcggcgagaa
     1201 tgcgctcgcg caggcgggac tcgacgacga aatacatgtc gttcatcagg tcgggcggca
     1261 ggtcggcgcg gttcaccacg acttcgtgca ggtcgggatt gacctgggcg cgctgcacca
     1321 ccgcctcgga cgcggcgcgc gacaggcggg cgccgtcatt ggacagaagc gtgtgcaggg
     1381 tcgcgtcgtc gccgcgcttg acgatcacgc cggagacgct ttcgggcacc tcctcgcggc
     1441 gggacacggc gcgcaaatgg tcctgaccct gggtcgagac gatatgcacg aggtcgcttt
     1501 cgctcagcgc ccgcgagcgc gtcaggatcg gttcagctac ggcgatctcg tccgaggcca
     1561 gcttgcgcaa caggcccagc ggcgccgtcc gggcgcccgc gatggcgccc gcgatctcgg
     1621 cgcgcacggc gctttccatg tcggcggaca actggctcat caccgaatcg tagagcgcca
     1681 tctcctgctc gcccacctgt tcgggatggg tcatgaacac gtcggtcacc ttgcgcagca
     1741 gttcgcggcg cttggcgctg gacggttcct tggccagttc gatgaggtcg gtcagggtcg
     1801 aggtctgcac gcgggacatg ctttctgcct ttagggggag gccttctggc cgttgttcgc
     1861 atccggggtc gaatctgcgg tcgaatccga atccgaggtc gaatcgtcgt cgcttttggc
     1921 gctcttgtcg ggcgcgacgg tcaggagcgc gccggtggcg ttgtcgtcca ccgtgatcgg
     1981 gtccggcttg tagccgtatt cgcgatgcag gctgtagaag cgcggcgcgt ccgaggcgtc
     2041 ggtgtagtgt tcgccgggaa tgaagacgtg gtcggtttcc tgcgaggcca gagacggcgc
     2101 cggcgcgggg acggtcgcgg cgggcttggc cgcggttt
//
LOCUS       DASVLL010000031         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_1766, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000031 DASVLL010000000
VERSION     DASVLL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VF402_00510"
     CDS             <1..144
                     /locus_tag="VF402_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080451.1"
                     /note="CysK; forms a complex with serine acetyltransferase
                     CysE; functions in cysteine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX7798796.1"
                     /translation="MKLARDLGPGKTVVTVLADYGNRYASKIFNPAFLKEKGLPTPPW
                     MAA"
     gene            complement(159..1121)
                     /gene="gcvA"
                     /locus_tag="VF402_00515"
     CDS             complement(159..1121)
                     /gene="gcvA"
                     /locus_tag="VF402_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEX7798797.1"
                     /translation="MSRDRLPPLSALRVFEAAARHESFKQAAEELFVTPGAVSQQIRL
                     LEEHVGVELFVRDGRRVVLSDAGKASAAILREAFEKMFEATRLMKAGVTKGRVTVSAA
                     PSFAAKWLMPRLHDFSAANPDIDVWVSADMAPVDFAVTDIDLAVRYGNGHYPNLHVEH
                     LLAESVVPVCSPLLFEAAPIRKAADLEHHVLLHDVGFETDPSCPDWAMWLKAHGVEGV
                     DATRGPRFNQSSMVIEAAVAGRGVALAKRTIAEADLQSGRLKVLLDEKDAPLQYGYYL
                     VWPQTREPGPGQVRFMDWLRAEAAQTDAPTRTDGERPVFAAMDI"
     gene            1208..2296
                     /locus_tag="VF402_00520"
     CDS             1208..2296
                     /locus_tag="VF402_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7798798.1"
                     /translation="MTDANPLHHVEALEAGQTWTTDVVVIGAGPVGLFSVFELGLLDI
                     KCHLVDILDKDGGQCAELYPEKPIYDIPAWPIISGDDLTKRLLEQIHPFGAKYHLGEM
                     AVAIRKLEDEDLWELTTDQKTVIRAKCIVIAAGGGSFQPKKPPIPGIDGFENLGAGKG
                     VHYAVRKMEAFRDKEIVISGGGDSALDWVLNLQPIAKKVTLIHRRDDFRAAPHSVAKM
                     RELVAEGKMDLVIAQATALKGETSHYLDAVLIEDANGVESELKADAFLPFFGLTMKLG
                     PVAEFGLNLHENLIPVDTEKFETSTPGVFAIGDINHYPGKLKLILSGFHEAALMAQKA
                     FKYVYPDKKLRFQYTTSSSELQKKMGVK"
     gene            complement(2346..2732)
                     /locus_tag="VF402_00525"
     CDS             complement(2346..2732)
                     /locus_tag="VF402_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7798799.1"
                     /translation="MKLLLDTHLLLWAAGLPERLPLAAARLIEDEANTLLFSAASLWE
                     VAIKSGLGREDFRVDARLLRRGLLDNGYEELPILSAHAVMVDTLPEIHKDPFDRMLVA
                     QAQHEGITLLTSDAMAARYPGPVRLV"
     gene            complement(2729..>2938)
                     /locus_tag="VF402_00530"
     CDS             complement(2729..>2938)
                     /locus_tag="VF402_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7798800.1"
                     /translation="AKTHLSRLLDQAAKGDAVVIAKAGKPVAKLTAINAPAPRRRLGF
                     LAGEVRVPDDFDRMGEGEISGLFGL"
BASE COUNT          563 a         1033 c          868 g          474 t
ORIGIN      
        1 atgaagctgg cgcgcgacct cggccccggc aagaccgtcg tgaccgtctt ggccgattac
       61 ggcaaccgct acgcctccaa gatcttcaac cccgccttcc tgaaggaaaa gggcctgccc
      121 accccgccgt ggatggcggc atgagggctt tggcggcgct agatatccat cgccgcgaac
      181 accggccgct cgccgtccgt gcgggtgggc gcgtccgtct gcgcggcctc ggcgcgcagc
      241 cagtccatga agcgcacctg ccccggcccc ggttcgcgcg tctgcggcca gacgaggtaa
      301 tagccgtact gcaacggcgc gtccttctcg tcgagcagca ccttgagccg tcccgactgc
      361 agatccgcct cggcgatggt gcgcttggcc agggcgacgc cgcgcccggc caccgctgcc
      421 tcgatcacca tggacgactg gttgaagcgc gggccgcgcg tggcgtccac gccctcgacg
      481 ccatgcgcct tcagccacat cgcccagtcc gggcaggacg gatcggtctc gaagccgaca
      541 tcgtgcagca gcacatgatg ctccagatcg gcggccttgc ggatcggcgc cgcctcgaac
      601 agaagcggcg aacacaccgg cacgaccgat tcggccagca gatgctcgac atgcaggttc
      661 ggatagtgcc cgttgccgta acgtacggcc agatcgatgt cggtcacggc gaagtccacc
      721 ggcgccatgt cggcgctgac ccagacgtcg atatcgggat tagccgcgct aaagtcatgc
      781 aggcgcggca tcagccactt ggccgcgaag ctcggcgcgg cggacacggt gacgcgcccc
      841 ttggtcacgc ccgccttcat cagccgcgtc gcctcgaaca tcttttcgaa cgcctcgcgc
      901 aatatggcgg cggacgcctt gcccgcgtcg ctcagcacca cgcggcgccc gtcgcgcacg
      961 aacagctcga ccccgacatg ctcctccagc aggcgaatct gctgcgacac cgcgccgggg
     1021 gtgacgaaca gctcctcggc ggcctgtttg aagctttcat gccgggcggc ggcttcgaac
     1081 acgcgcaggg cggacaaggg cggcaaacgg tcgcgtgaca taacgggtaa acattccttt
     1141 caaccggcgc ttccttatag cgcttttcag tttaagaaaa ctatccgata cggatttgga
     1201 gacgattatg acggacgcca acccccttca ccatgtcgaa gcgctggaag cgggccagac
     1261 ctggaccacc gatgtcgtgg tgatcggcgc agggccggtg ggcctgttca gcgtgttcga
     1321 actgggcctg ctcgacatca agtgccacct ggtcgacatt ctcgacaagg acggcggcca
     1381 gtgcgccgag ctttatcccg aaaagccgat ctacgacatt ccggcctggc cgatcatctc
     1441 cggcgacgac ctgaccaagc gccttctgga gcagattcac ccgttcggcg ccaagtacca
     1501 tctcggcgaa atggccgtgg cgatccgcaa gctcgaagac gaggatttgt gggaactgac
     1561 caccgaccag aagaccgtga tccgcgccaa atgcatcgtg atcgcggcgg gcggcggctc
     1621 gttccagccg aaaaagccgc cgatccccgg catcgacggc tttgaaaacc tcggcgcggg
     1681 caagggcgtg cattacgccg tgcgcaagat ggaggccttc cgcgacaagg agatcgtcat
     1741 ttccggcggc ggcgattccg cgctcgactg ggtgctcaac ctgcaaccga tcgccaaaaa
     1801 ggtgaccctg atccaccgcc gcgacgactt ccgcgccgcg ccgcactcgg tcgccaagat
     1861 gcgcgaactg gtcgccgaag ggaagatgga cctcgtgatc gcccaggcca ccgcgctcaa
     1921 gggcgagacc agccactatc tcgacgccgt gctgatcgag gacgccaacg gcgtggaatc
     1981 cgaactgaaa gccgacgcct tcctgccctt cttcggcctg accatgaagc tcggccccgt
     2041 cgcggaattc ggcctcaacc tgcacgaaaa cctgatcccg gtcgacaccg agaagttcga
     2101 aacctccacc cccggcgtct tcgccatcgg cgacatcaac cactatccgg gcaagctgaa
     2161 actgatcctg tccggcttcc acgaagccgc cctgatggcg caaaaagcct tcaaatacgt
     2221 ctatcccgac aagaaactgc gcttccagta cacgacctcg tcgtccgaac tgcaaaagaa
     2281 gatgggcgta aaatgatccc tatacctccc tgaacgcgca gcgtttgggg aggtataggc
     2341 tgctgtcaca ccagccgcac tggccccgga tagcgcgccg ccatcgcgtc cgaggtcagg
     2401 agcgtgatcc cttcatgctg cgcctgcgcc accagcatcc ggtcgaacgg gtccttgtga
     2461 atctccggca acgtatccac catgaccgca tgcgccgaca ggatcggcag ttcctcatag
     2521 ccgttatcga gcagcccccg ccgcaacagg cgcgcatcga cgcggaaatc ctcgcgcccc
     2581 aggccgctct tgatcgccac ctcccacagg ctggcggcgc tgaacagaag cgtattcgcc
     2641 tcgtcctcga tcagccgcgc cgccgccagc ggcaatcgct ccggcaaccc cgccgcccac
     2701 agcagcagat gcgtatcgag cagtagtttc acaggccgaa caatccggaa atctcgcctt
     2761 cgcccatccg gtcgaaatcg tccggcacac gcacctctcc ggccagaaaa ccgagacgcc
     2821 ggcgcggcgc gggcgcgttg atggcggtga gcttcgctac cggtttcccg gccttggcga
     2881 tcaccacggc gtcgcccttc gccgcctgat ccagcaggcg cgacagatgc gtcttggc
//
LOCUS       DASVLL010000032         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_163070,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000032 DASVLL010000000
VERSION     DASVLL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163070"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VF402_00535"
     CDS             <1..610
                     /locus_tag="VF402_00535"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX7798801.1"
                     /translation="LAACQRQPPPHGEEQAAASASASASASAAEASEVSDSAAASSPE
                     AAETEAIGPLGQKPGLWKLTQTLPQMEATSVSKICIDAEQGERLAMIGQDLLGKPEMA
                     RLSCTRRGVVTRDGRLEADTVCMVNDTTLSSHIHADVIGHRVFHQTVETRYTPAFAGH
                     GDQITVTDASWQGACPDGMAPGDFVNRDGVRSNVSKLLAKFR"
     gene            complement(679..1122)
                     /locus_tag="VF402_00540"
     CDS             complement(679..1122)
                     /locus_tag="VF402_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7798802.1"
                     /translation="MSDACPPELRLDNQLCFAVYATAHAFAQAYRPHLEPMGLTYPQY
                     LVMLLLWERDGRSVTELGAPLHLDSGTLTPLLKRMEKAGFVTRRRDARDARVTRVHLT
                     PHGKALREPARAIPAAMACAANLAPDDMLALRDRIAAVGDAMRNA"
     gene            1207..1635
                     /locus_tag="VF402_00545"
     CDS             1207..1635
                     /locus_tag="VF402_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX7798803.1"
                     /translation="MTQSITPLYTAHAAATGGREGTAHTDDNRVSLTLSTPKELGGAG
                     GEGTNPEQLFAMGYAACFVGALKFVGARDKVKVPADTTIRSSVGIGQIPAGFGINVKL
                     EISLPGVEAETADRLIAEAHKVCPYSNATRGNIDVELTRA"
     gene            1714..2595
                     /locus_tag="VF402_00550"
     CDS             1714..2595
                     /locus_tag="VF402_00550"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX7798804.1"
                     /translation="MADDLNGEAAGAADEIDAAHLEAMGDNLALAQTKAARPVDAISG
                     QPLKKKDAVRLDDLRPALADYIRAHHPQVGGDDYVSRKTLDDYRGRYIAGLLSMERGE
                     LSNLETEVVESLKTHDTLAENIEEDYEDHRSLGDRVADVVAAFGGSWTFILSFCFFLA
                     VWMGINLWLGEKRAFDVYPFILLNLILSTIAALQAPVIMMSQRRQEEKDRLRALNDYK
                     VNLKAELEIRHLHEKVDHLLNRQWERLTEIQQIQIEMMLEQAGKAQKIVKVRKVIKRK
                     PRKKPAAKTAPEAEAGA"
     gene            complement(2599..>3194)
                     /locus_tag="VF402_00555"
     CDS             complement(2599..>3194)
                     /locus_tag="VF402_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7798805.1"
                     /translation="VLIDTGLGYRYHGVLLGSLAKAGVAPEAVTDVLITHAHPDHIGG
                     LVSQDGESVFPRAKVRMAAAEWDWVKSQPDLAAFVSVMGPQVETFAPGAEVAPGVVAI
                     ALPGHTPGHTLYEIGSNGARLIDIGDTAHSSIVSLEKPDWKIQFDTDQAEGAAQRRAE
                     LTRLAASGETVFAPHFPFPGIGTVARDGDGFTWIPAK"
BASE COUNT          592 a         1048 c         1077 g          477 t
ORIGIN      
        1 gctggcggcg tgccagcgcc agccgccgcc gcatggcgaa gaacaggcgg cggcctccgc
       61 ctccgcctcc gcctcggcgt ccgccgccga agcgtccgag gtttcggaca gcgccgccgc
      121 gtccagcccc gaagcggcgg agacagaggc catcggcccg ctgggccaga agccgggcct
      181 gtggaagctg acccagaccc tgccgcagat ggaggcgacc tcggtgtcga agatctgcat
      241 cgacgccgag cagggcgagc ggctggccat gatcggccag gacctgctgg gcaagccgga
      301 gatggcgcgg ctgagctgca cccggcgcgg cgtcgtcacg cgcgacggac ggctcgaagc
      361 cgacacggtc tgcatggtca acgacacgac gctgagcagc catatccacg ccgacgtcat
      421 cggccaccgg gttttccacc agacggtcga gacgcgctat acgcccgcct tcgccgggca
      481 tggcgaccag atcaccgtca ccgacgccag ttggcagggc gcctgtcccg acggcatggc
      541 gccgggcgat ttcgtcaacc gcgacggcgt gcgctcgaac gtgtcgaagc tgctggcgaa
      601 attccgctag agcgtttccc caaaaagcag ctctactttt tggacaagga aggtcgacaa
      661 aacaaagact tagagcgctt aagcgttacg catggcgtcg ccgaccgcgg cgatgcggtc
      721 gcgcagggcc agcatgtcgt ccggggccag attggccgcg cacgccatcg ccgccgggat
      781 ggcgcgcgcc ggttcgcgca gcgccttgcc gtggggggtg agatggacgc gcgtgacgcg
      841 cgcgtccctg gcgtcgcggc ggcgggtgac gaaaccggcc ttctccatgc gtttcagcag
      901 cggcgtcagc gtgcccgaat cgagatgcag cggcgcgccc agttcggtga ccgaacgccc
      961 gtcgcgctcc cacaacagca gcatgaccag atattgcgga taggtcagcc ccatcggctc
     1021 cagatgcggc cggtaggcct gcgcgaaggc gtgcgcggtc gcatagacgg cgaagcaaag
     1081 ctggttgtcg agcctgagtt cgggcgggca tgcgtcggac atgacgatta tatagcgcac
     1141 aatcttcttg acatcaaatt gtacacaata taattgttgg ctatcaatta gccgaaggag
     1201 gccgtcatga cccagtccat caccccgctc tacaccgccc acgccgccgc gaccggcggc
     1261 cgcgaaggaa ccgcgcacac cgacgacaac cgcgtgtcgc tgaccctgtc gacgccgaag
     1321 gagctgggcg gcgcgggcgg cgaggggacc aacccggaac agttgttcgc catgggctat
     1381 gcggcctgct tcgtgggcgc gctaaaattc gtcggcgcgc gcgacaaggt gaaggtgccc
     1441 gccgacacga cgatccggtc gagcgtcggc atcggccaga tccccgccgg tttcggcatc
     1501 aatgtgaagc tggagatctc gctgccgggc gtcgaggcgg agaccgccga ccgcctgatc
     1561 gcggaggcgc acaaggtctg tccgtactcc aacgccacgc gcggcaatat cgacgtcgaa
     1621 ctcacgcgcg cttaacaagg ggttgtcata atcctgtcgc ccggctgtca taagtcgcgg
     1681 acaatgtcga atcgcgggag tcgggtgacg gatatggcgg atgatctgaa cggcgaggcc
     1741 gccggggcgg cggacgagat cgacgccgcg catctggagg ccatgggcga caatctggcc
     1801 ctggcccaga ccaaggccgc gcgtccggtc gacgccatca gcggccagcc gctgaaaaag
     1861 aaggacgccg tgcgtctcga cgacctgcgc ccggccctgg ccgactatat ccgcgcgcat
     1921 cacccgcagg tcggcggcga cgactatgtc agccgcaaaa ccttggacga ctatcgcggg
     1981 cgctatatcg ccggcctgct cagcatggag cgcggcgagc tgtccaacct cgagaccgag
     2041 gtggtggaaa gcctgaaaac ccatgacacc ctggccgaga acatcgaaga ggactacgag
     2101 gaccaccgca gcctcggcga ccgcgtggcc gacgtggtcg cggccttcgg cggcagctgg
     2161 accttcatcc tgtccttctg cttctttctg gcggtgtgga tggggatcaa tctgtggctg
     2221 ggcgaaaagc gcgccttcga cgtctatccg ttcatcctgc tcaacctgat cctgtcgacc
     2281 atcgccgccc tgcaggcgcc ggtcatcatg atgagccagc gccgtcagga ggaaaaggac
     2341 cgcctgcgcg cgctcaacga ttacaaggtc aatctcaagg ccgagctgga gatccgccac
     2401 ctgcacgaaa aggtcgacca cctgctgaac cgccagtggg agcggctgac cgaaatccag
     2461 cagatccaga tcgagatgat gctggagcag gccggcaagg cgcagaagat cgtcaaggtg
     2521 cgcaaggtga tcaagcgcaa gccgcgcaag aagcccgccg cgaaaacggc gccggaagcc
     2581 gaagccgggg cgtaagcctt acttcgctgg aatccatgtg aagccgtcgc cgtccctggc
     2641 gacggttccg atgccgggga aggggaagtg cggcgcgaac accgtctcgc cgctggcggc
     2701 cagccgggtc aattcggcgc ggcgttgagc ggcgccctcc gcctgatcgg tgtcgaactg
     2761 aatcttccag tcgggtttct ccagcgagac gatgctggaa tgggcggtgt cgccgatatc
     2821 gatcagccgc gcgccgttcg agccgatctc gtacagggta tgccctggcg tgtggccggg
     2881 cagggcgatg gcgacgacgc ccggcgcgac ctcggcgccc ggcgcgaagg tttcgacctg
     2941 cggccccatc acgctgacga aggccgccag atcgggctgg gacttcaccc agtcccattc
     3001 ggcggcggcc atgcgcacct tcgccctggg gaagacggac tcgccgtcct ggctcacgag
     3061 gccgccgata tgatcgggat gcgcgtgggt gatcagcacg tcggtgaccg cttcgggcgc
     3121 gacgcccgcc ttggcgaggc tgcccagcag cacgccgtga tagcgatagc cgaggcccgt
     3181 gtcgatcagc acga
//
LOCUS       DASVLL010000033         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_431767,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000033 DASVLL010000000
VERSION     DASVLL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431767"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1688
                     /locus_tag="VF402_00560"
     CDS             213..1688
                     /locus_tag="VF402_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX7798806.1"
                     /translation="MRVAAGDQVAVALEDLQAGQSVSAGGVTLTLRDDVPRGHKFALI
                     PIAAGEAVSKYGFRIGTASADIAPGQHVHSHNLRTALEGLEDYVYAPDAPARGEGATG
                     TFQGYRRGWGRSGGGVGIRNEIWILCTVGCVARTAQRLAKEAHERFKGRVDGVYAITH
                     PLGCSQLGDDLGHTRDVLAALASHPNAGAALIVGLGCENNQLSELIKRIDRPEDRLAW
                     FAAQKVDDELEAGMNALEKLVAVIENDRRETCDVSDLVIGVKCGGSDAFSGLTANPVV
                     GRVADKVAFAGGKAILSEIPEIFGAERLLMARARDRATFQGVVDVVNDFKTYFIEHKQ
                     PVFENPSPGNKAGGLTTLEEKSLGAVQKGGTSPLNGVLRYGERVRESGLSLLEAPGND
                     AVSSTALTAAGAVIVLFTTGRGTPLGFPAPTLKIASNSVLAAHKPHWIDFDAGVVLDA
                     GVDGAADALMDKILSVASGAPTRNEINDEREIAIWKNGVTL"
     gene            1685..>2135
                     /locus_tag="VF402_00565"
     CDS             1685..>2135
                     /locus_tag="VF402_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX7798807.1"
                     /translation="MTQLNLTALSSLSPSVSGPSYDPASVRIGIVHFGPGAFFRAHQA
                     AYVDALLAKDGRWGICGVSLHSAGVRDALVPQDGLYTLAVLDEAISYRVIGSLREVLV
                     APEDPEAVFARLTRPEVRVVTSTVTEKGYCLGAGFALDFDHPDIAHDL"
BASE COUNT          334 a          645 c          795 g          361 t
ORIGIN      
        1 tgtcacgccg atttatgtcc ctatatgaaa tttacgaccc catatgaaaa gacagcttgc
       61 ctttgggggg cgatcggatt tatgacaccg gttaccatag ccggggtccg cctccggcgc
      121 aaggtttctg tttctgagcg attgcaaggt gcgtcatgtc cactctcact gattcccgat
      181 ccggcgattc acgggccggc ggccaccatg ccatgcgcgt cgccgccggc gatcaggtgg
      241 cggtggcgct ggaggatttg caggcgggcc agagcgtgag cgcgggcggc gtcacgctga
      301 cgctgcgcga cgacgtgccg agggggcaca agttcgcgct gattcccatc gcggcgggcg
      361 aggcggtcag caagtacggc ttccgcatcg ggacggccag cgccgatatc gcgccgggcc
      421 agcacgtcca cagccacaat ctgcgcaccg cgctggaggg gctggaggac tatgtatatg
      481 cgccggacgc gcccgcgcgc ggcgagggcg ccacggggac gtttcagggc tatcgccgcg
      541 gctgggggcg ttccgggggc ggcgtcggca tccgcaacga aatctggatt ctctgcacgg
      601 tcggctgcgt ggcgcgcacg gcgcagaggc tggcgaagga ggcgcatgag cgtttcaagg
      661 gccgggtcga cggggtttac gccatcaccc atccgctcgg ctgctcgcag cttggcgacg
      721 acctcggcca tacgcgcgac gtgctggccg cgctggcctc gcatccgaac gccggggcgg
      781 cgctgatcgt cgggctgggc tgcgagaaca accagttgtc ggagctgatc aaacgcatcg
      841 accggccgga ggaccggctg gcctggttcg ccgcgcagaa ggtcgatgac gagcttgagg
      901 cgggcatgaa tgcgctggaa aagctggtcg cggtcatcga gaacgaccgg cgcgagacgt
      961 gcgatgtgtc ggacctcgtg atcggggtga agtgcggcgg ttcggacgcc ttttccggcc
     1021 tgaccgccaa tccggtggtc gggcgcgtgg cggacaaggt ggccttcgcg ggcggcaagg
     1081 cgatcctgag cgaaatcccg gaaattttcg gcgccgagcg cctcctgatg gcgcgcgcca
     1141 gggaccgcgc gacctttcag ggcgtcgtcg acgtcgtcaa cgatttcaag acctacttca
     1201 tcgagcataa gcagccggtg ttcgagaacc cgtcgccggg caacaaggcg ggcgggctga
     1261 ccacgctgga ggaaaagtcg ctcggcgcgg tgcagaaggg cgggaccagc ccgctcaacg
     1321 gcgtgctgcg ctacggcgag cgcgtgcggg aaagcggcct gagccttctg gaggcgccgg
     1381 gcaacgacgc ggtgtcgtcg acggcgctga cggcggcggg ggcggtgatc gtgctgttca
     1441 ccacgggacg cggcacgccg ctgggctttc cggcgccgac gctcaagatc gcctcgaact
     1501 cggtgctggc ggcgcataag ccgcactgga tcgacttcga cgccggggtc gtgctggacg
     1561 cgggcgtgga cggcgcggcg gacgcgctga tggacaagat tttgtcggtg gcctcaggcg
     1621 cgccgacgcg caacgaaatc aacgatgagc gcgagatcgc catctggaag aacggagtca
     1681 ccctgtgacg cagcttaacc ttaccgccct gtcctctctg tcgccttcgg tgtcgggtcc
     1741 gtcctatgac ccggcttcgg tcaggatcgg gatcgtgcat ttcgggccgg gggcgttttt
     1801 ccgggcgcat caggcggcct atgtggacgc cttgctggcg aaggacggtc gctggggaat
     1861 ctgcggggtg tcgctgcatt cggcgggggt gcgcgacgcg ctcgtcccgc aggacgggct
     1921 ttacacgctg gcggtgctgg acgaggcgat cagctatcgc gtgatcggtt ccctgcgcga
     1981 ggtgctggtc gcgccggaag acccggaggc ggtgttcgcg cggctgacgc ggcccgaagt
     2041 gcgggtggtg acctcgacgg tgacggagaa gggctattgc ctgggggcgg gcttcgcgct
     2101 ggacttcgat catcccgaca tcgcgcacga tctgg
//
LOCUS       DASVLL010000034         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_485240,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000034 DASVLL010000000
VERSION     DASVLL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485240"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..444)
                     /gene="rpsP"
                     /locus_tag="VF402_00570"
     CDS             complement(10..444)
                     /gene="rpsP"
                     /locus_tag="VF402_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7798808.1"
                     /translation="MLKIRLSRGGSKKRPYYTIVVANATAPRDGKFIEKVGTYNPMLP
                     KDAQRVTLKAERIAEWLKKGAQPTDRVARFLSQEGLVTWQHGNNPKKGLPGKKAQERA
                     AERAQREADRLEAEAAAKAEAEAAAAAPAAEEAPAEEAAASE"
     gene            complement(469..2055)
                     /gene="ffh"
                     /locus_tag="VF402_00575"
     CDS             complement(469..2055)
                     /gene="ffh"
                     /locus_tag="VF402_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7798809.1"
                     /translation="MFDTLNERLSGLFDSLSGRGVLSEKDVEAALREVRVALLEADVA
                     LPVVRQFMTKAKELATGEAIIKAVKPADQVVKIVYDGLIDMLGGETPEPINLAAVPPV
                     VVLMAGLQGSGKTTTSAKLALRLKNFDRKKVMLASLDTRRPAAMEQLKLLAEQVGVEC
                     LPIVAGQSAVEITKRALTAAKLQGFDVLILDTAGRTTLDEELMSEAAQIAKIAQPTET
                     FLVADSLTGQDAVRTATAFHERLPLTGLIMTRADGDSRGGAMLSMRAVTGLPIKYLGA
                     GEKVDALDVFDARRIAGRILGQGDIVALVEKAAQELDAAKTAAMARKMAKGQFDLDDL
                     ADQLRQMKKLGGMSGIMGMLPGVQKVKKQMDEMNLSEKIFDRQVAIITSMTRAERKKP
                     DLLNASRKRRVAAGAGVEVAEINRLLKQHRQMADTFKMMARSNGRGPMGQLAKMFGGQ
                     MGGLSGGLGGGLPPEANMGELAEMAKKMSEGAPTGANPLTGVNPALDLEKLKNLGAGK
                     SPFAGLPGLPGAKPFDPTRK"
     gene            complement(2260..2715)
                     /locus_tag="VF402_00580"
     CDS             complement(2260..2715)
                     /locus_tag="VF402_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7798810.1"
                     /translation="MDETENRIVAEHSRALGWLLANAHGLYRARLAAAIADEPVHMGQ
                     VVLLASLYAENDLTQAQLSKVSGIEKSSIVLFLDALEKDGWVERRRHPGDRRAHLVHL
                     TEDGRARFTQVGLKLKAAQDAALSVFAPDERAAFEGYLLRLIGHLTASA"
     gene            2817..3788
                     /locus_tag="VF402_00585"
     CDS             2817..3788
                     /locus_tag="VF402_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798811.1"
                     /translation="MWRRLPAALSLFVLSPLIAEYLLGSMPMAMIALLPLMAAMYGSG
                     AVLIREAVRRAGRGWPSMILLATAYGLIEEGFVTQSLFNPNYLHLRLLDYGYVPWLGT
                     GLPWLVYVISIHVVWSMSVPIGLSEGLFRNRRDQPWLGPVGIGAFALLFAAGSALIAA
                     YTYKSLPFMASPAQLAATGGAVALLIAAAFALPKTAPASDRAAPHPLVLLGAGVIAGS
                     ALMLLQLLAQHGWRWPWPACVAALVLVEAGFVAFMIACTRGRAWTDLRRCALMSGGLL
                     VYGWSGFVIARDLHGAADLPGHAVIAGLFALLLGWAWVVAARPAKRP"
     gene            complement(3715..>4749)
                     /locus_tag="VF402_00590"
     CDS             complement(3715..>4749)
                     /locus_tag="VF402_00590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7798812.1"
                     /translation="ARLPGKGGVWFLHANAPECLVAFLAHAPGQWADRYRIGYWAWET
                     PRAPEEWVWLADYLHEIWVPSRFVRDALSAAFAAARRDDLNDKLRVMPHPVPVSPPAR
                     YDYVHQRFGMTPELCEVLCLFDVKSSAARKNPWAVLDVWKRAFPAPSDTARLTLKVSD
                     LSGDEATQRRLLRELKGRPDIRLFEERLSAADMQAFLGGFDVLISLHRSEGFGLPLAE
                     AMGAQTAVIATGWSGNLDFMTGDNSRLIPARLVPLRDPDGPYCRQSGRPGQVWAEPDR
                     IAAIDALLELARSEAKRRDLAYAGLRAVRDLSEPWRREALDALPFRAFLPDAPPQPTP
                     SPAKARKARR"
BASE COUNT          678 a         1646 c         1559 g          866 t
ORIGIN      
        1 tgacgccgct tattccgaag cggcggcttc ctcggccggg gcttcctcgg cggcgggcgc
       61 ggcggcggcg gcttcggctt cggccttggc ggccgcttcg gcttccagac ggtcggcttc
      121 gcgctgggcg cgctcggcgg cgcgttcctg cgccttcttg cccggcaggc ccttcttcgg
      181 gttgttgccg tgctgccacg tcaccagacc ctcttgcgac aggaagcgcg cgacgcggtc
      241 ggtcggctgg gcgcccttct tcagccattc ggcgatgcgc tccgccttaa gcgtgacgcg
      301 ctgggcgtcc ttgggcagca tcgggttgta cgtgccgacc ttttcgatga acttgccgtc
      361 gcgcggcgcg gtcgcgttgg cgacgacgat ggtgtagtag gggcgcttct tggagccgcc
      421 gcgggacaaa cggattttca gcattgtgtt ggattccttt gaaagaggct atttcctggt
      481 gggatcgaag ggtttggcgc cgggcaggcc cggcaaaccg gcgaagggcg atttgcccgc
      541 gccgaggttt ttcagtttct caaggtccag cgccggattg acgccggtga gcggattggc
      601 gccggtcggc gcgccttcgc tcatcttttt ggccatctcg gccagttcgc ccatattggc
      661 ttccggcggc aggccgccgc ccagaccacc gcttagaccg cccatctggc cgccgaacat
      721 cttggccagt tgccccatcg ggccgcgccc gttggagcgc gccatcatct tgaaggtgtc
      781 ggccatctgg cggtgctgtt tcaaaaggcg gttgatctcc gccacctcga ccccggcgcc
      841 cgccgcgacg cggcgcttgc gcgaggcgtt gagcaggtcg ggcttcttgc gctccgccct
      901 ggtcatcgag gtgatgatcg ccacctgacg gtcgaagatc ttttccgaca ggttcatctc
      961 gtccatctgc ttcttgacct tctgcacgcc gggcaacatg cccataatgc ccgacatgcc
     1021 gcccagcttc ttcatctggc gcaactggtc ggcgaggtcg tcgaggtcga actgcccctt
     1081 ggccatcttc ctggccatgg cggcggtctt ggcggcgtcc agctcctgcg cggccttttc
     1141 gaccagcgcg acgatgtcgc cctgccccaa aatgcggccc gcgatgcggc gcgcgtcgaa
     1201 cacgtcgagc gcgtcgacct tttcaccggc gcccagatac ttgatcggca ggccggtgac
     1261 ggcgcgcatc gacagcatgg cgccgccgcg cgaatcgccg tcggcgcggg tcatgatcag
     1321 gccggtcagc ggcaggcgct catggaaggc cgtggcggtg cgcaccgcgt cctgaccggt
     1381 caggctgtcg gcgacgagga aggtttcggt cggctgggcg atcttcgcga tctgcgccgc
     1441 ctcgctcatc agctcctcgt ccagcgtggt gcggcccgcc gtgtcgagga tcagcacgtc
     1501 gaagccttgc agcttcgcgg cggtcagggc gcgcttcgtg atctcgaccg cgctctggcc
     1561 cgccacgatc ggcaggcact cgacgccgac ctgctcggcc agcagcttca actgctccat
     1621 cgccgccgga cggcgcgtat cgagcgaggc cagcatgacc ttcttgcggt cgaaattttt
     1681 caggcgcagg gccagcttgg ccgaggtggt cgtcttgccc gagccttgca aacccgccat
     1741 cagcaccacg accggcggca cggcggcgag gttgatcggc tccggcgtct cgccgcccag
     1801 catgtcgatc aggccgtcat agacgatctt gaccacctga tcggcgggct tgaccgcctt
     1861 gatgatcgcc tcgcccgtgg ccagctcctt ggccttggtc atgaactggc gcacgaccgg
     1921 cagggcgacg tcggcctcca gcagcgccac gcgcacttca cgcagcgccg cctcgacgtc
     1981 cttttccgaa aggacgccgc gccccgaaag gctgtcgaac agaccggaca ggcgctcgtt
     2041 aagcgtgtcg aacatgagcc agaaacctct atcttctgcg cccggccctg cattccccgc
     2101 aaacaaaaaa cccccgaggg cgcaacgcgc tgtcggggag ccttcccagc ctctgcggat
     2161 ctaaatccgt tcccgtgccg gtgaaggggt ctgttctcgg agcgcgcaaa gccttacgcg
     2221 cggatgtggc cggttcaatg gcggaaaagg cgcaaagagt caagccgacg cggtcaaatg
     2281 cccgatcagg cgcagaagat agccctcgaa cgccgcgcgc tcgtccggcg caaacaccga
     2341 caaagccgcg tcctgcgccg ccttgagctt caggccgacc tgcgtgaagc gggcgcggcc
     2401 gtcttcggtc agatgcacca gatgggcgcg gcggtcgccg gggtgacggc ggcgctcgac
     2461 ccagccgtcc ttttccagcg cgtcgagaaa cagcacgatg gacgatttct cgatgccgga
     2521 caccttcgac aactgcgcct gcgtcaggtc gttttcggcg tacaggctgg ccagcagcac
     2581 cacctgcccc atatgcaccg gttcgtcggc gatggccgcc gccagacgcg cgcggtaaag
     2641 gccgtgcgcg ttcgccagca gccagcccag cgcccggctg tgttcggcga cgattctgtt
     2701 ttccgtctca tccatgaccc cagccatagg cggcggcggc ttgggcgtca atatcgtcca
     2761 cttgcatatt gtctatttat agattatcta tctacgcaca ttttaaggag gctctcatgt
     2821 ggcgtcgtct gcccgcggcc ctgtcgctgt tcgtcctgtc gccgctgatc gcggaatatc
     2881 tgctcggttc gatgccgatg gcgatgatcg ccctcctgcc gcttatggct gcgatgtacg
     2941 gctccggcgc ggtgctgatc cgcgaagccg tgcgccgcgc cggacgcggc tggccgtcga
     3001 tgatcctgct ggcgaccgcc tacgggctga tcgaggaggg gtttgtcacc cagtcgctgt
     3061 tcaacccgaa ctatctgcac ctgcgcctgc tcgattacgg ctatgtcccg tggctgggca
     3121 cgggcctgcc gtggctggtc tatgtgattt cgatccatgt cgtctggagc atgagcgttc
     3181 cgattgggct gagcgaaggc ctgttccgca accggcgcga ccagccgtgg ctcggccccg
     3241 tcggcatcgg cgccttcgcg ctgctgttcg cagccggatc ggcgctgatc gccgcttaca
     3301 cctataaatc cctgcccttc atggccagcc cggcgcagct tgccgcgacc ggcggcgcgg
     3361 tcgcgctgct gatcgccgcc gccttcgccc tgccgaagac cgcgcccgct tcggatcgcg
     3421 cggcgccgca tccgcttgtc ctgctcggcg ccggtgtcat cgccggttcg gccctgatgc
     3481 tgctgcaact gctggcgcag cacggctggc gctggccgtg gcccgcctgc gtcgcggcgc
     3541 ttgtgttggt cgaggcgggc ttcgtcgcct tcatgatcgc ctgcacgcgc ggacgggcgt
     3601 ggacggattt gcggcgttgc gcgctgatga gcggcgggct gctggtctat ggctggagcg
     3661 gcttcgtcat cgcccgcgac ctgcacggcg cggcggacct gcccggccac gccgtcatcg
     3721 ccgggctttt cgcgcttttg ctgggctggg cgtgggttgt ggcggcgcgt ccggcaaaaa
     3781 ggccctgaac ggcaaggcgt ccagcgcctc gcgccgccac ggttcgctga ggtcgcgcac
     3841 cgcgcgcagc ccggcatagg ccaggtcgcg gcgcttcgcc tccgatcgcg ccagctccag
     3901 cagggcgtcg atggcggcga tgcggtccgg ctcggcccag acctgaccgg gtctgccgga
     3961 ctggcggcaa taggggccgt ccgggtcgcg cagcgggacc agccgcgccg ggatcaggcg
     4021 gctgttgtcg ccggtcatga agtcgagatt gccggaccag ccggtcgcga tcaccgccgt
     4081 ctgcgccccc atcgcctcgg ccagcggcag gccgaacccc tccgaccggt gcagcgagat
     4141 cagcacgtcg aagccgccga ggaaggcctg catgtcggcg gcgctcaggc gctcctcgaa
     4201 caggcggatg tcgggccgac ccttcagttc gcgcagcaga cgccgctgcg tcgcttcatc
     4261 gccgctcagg tccgagacct tcagcgtcag ccgcgccgta tcggacggcg ccggaaaggc
     4321 gcgcttccag acgtcgagaa ccgcccacgg attcttgcgc gccgccgacg atttgacgtc
     4381 gaacaggcac aggacttcgc acagctccgg cgtcatgccg aaacgctgat gcacatagtc
     4441 gtaacgcgcc ggcgggctga ccggcacggg atggggcatg acacgcaatt tatcgttcag
     4501 gtcgtcacgc cgcgccgccg cgaacgccgc gctcagcgcg tcgcgcacga accggctcgg
     4561 aacccagatc tcatgcagat aatccgccag ccagacccat tcctccggcg cgcgcggcgt
     4621 ctcccaggcc cagtagccga tacgatagcg gtccgcccac tgccccggag catgcgcgag
     4681 gaaggcgacc agacattccg gcgcattggc gtgcaaaaac cacacgccgc ccttgccagg
     4741 caagcgcgc
//
LOCUS       DASVLL010000035         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_109529,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000035 DASVLL010000000
VERSION     DASVLL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109529"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /gene="nadC"
                     /locus_tag="VF402_00595"
     CDS             <1..524
                     /gene="nadC"
                     /locus_tag="VF402_00595"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7798813.1"
                     /translation="CHLSGVASLTARYVAALDGLNARIVCTRKTLPSLRALQKHAVRC
                     GGGYNHRYGLDDAILIKDNHIAICGGVEAAMQRAKAGVGHTMKIEIEVDSIAQLKLAL
                     PFKPDVILLDNFTTDMFAQAVKLAAGKTLLEASGGVNLDTVRAIAETGVDVISVGALT
                     HSAPNFDIGLDAV"
     gene            577..831
                     /locus_tag="VF402_00600"
     CDS             577..831
                     /locus_tag="VF402_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7798814.1"
                     /translation="MKHVTYSAFRQNLAKLLDQVNDDHAPLLVTRQNGAPTVVVSLED
                     FTAWQETLYLRASPKNAERLSRSIERLNAGLGEAHDLIEE"
     gene            828..1082
                     /locus_tag="VF402_00605"
     CDS             828..1082
                     /locus_tag="VF402_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEX7798815.1"
                     /translation="MKIIFDPDAWEDYLYWQGHDKITVKRINALIKDIARHPFEGIGQ
                     PEALKYDWSGYWSRRIDQEHRFIYRVPPDAILIAQCRYHY"
     gene            1136..1366
                     /locus_tag="VF402_00610"
     CDS             1136..1366
                     /locus_tag="VF402_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7798816.1"
                     /translation="MQVAKWGNSLAVRLPAAVVEALELKVGDDIAIRAESRRDFVIER
                     QPTRDELIKRLDALRFRMPADYKFDREEANER"
     gene            1356..1766
                     /locus_tag="VF402_00615"
     CDS             1356..1766
                     /locus_tag="VF402_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7798817.1"
                     /translation="MSVKRGFIDTNVLVYALSEGNEKAARAAELRNAGGVISVQVLNE
                     LVNVLRRKLKHPWPETLQRLMIVKSLLEIVPLTLETHEAGLELARRYSLSLYDAMIVA
                     SALEAGCDALWSEDMQDGLRIGDLTIRDPFRRRP"
     gene            complement(1750..2871)
                     /locus_tag="VF402_00620"
     CDS             complement(1750..2871)
                     /locus_tag="VF402_00620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7798818.1"
                     /translation="MKPLNFSITGSAGTPIQARMGGASLDVLRFLAALFILLFHYGSN
                     APRDLETIVPVFRQGWLATDFFLMLSGYVLSRAYGRRLVESGMRRSHFFLRRFARLWP
                     SHVIVLLAFGAFVLASGAVGLMPKHPDKYGAFAFLAQVFMVHGWGMFAAPSWNVPTWT
                     ISALLICYGLFSFYAPWMWRLGRWRLAALALGVLAVAHLFALSVVRHPLADLPFEWGL
                     GRAIPLFVIGSLIERATSGLTTPKPVFWIGSVGVLLALPFLCLLPRNIGMDTLLLGLL
                     ALALAVSGGVTFPETTATRLMGRASFALFLVHSLVGAIWFGVTARLTAHFGLGAAAQW
                     ALWSLGVLLAVIAAFVFDALVDKPLSQRVSRLSFVKGGA"
     gene            complement(2897..3517)
                     /locus_tag="VF402_00625"
     CDS             complement(2897..3517)
                     /locus_tag="VF402_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7798819.1"
                     /translation="MDREDRREAILDVATDVFLKEGYASASMSTIAARVGGSKGTLYN
                     YFKSKEELFEAYVQRSCVFHRGEIAHLLTEEGDARTVLTRFARGYVRAFTAEPALQNW
                     RVISAESHKSPDVGRMFYEAGPLTGARIIAAYIEKARDRGDLIAPDVMTAAHHFTSLI
                     HGRMIKARLLNYMPEPTDAEIEAEVEGAIAVFFAAYGANAAAASKS"
     gene            3702..5111
                     /locus_tag="VF402_00630"
     CDS             3702..5111
                     /locus_tag="VF402_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7798820.1"
                     /translation="MTHLRPSVPLAAALLAATALTACTTLPQSAPYATEPSQAKKVNP
                     IAAITEQDWWKSFGDPQLDGLMTQALNNAPSLAMAEARLNAAQAVVSSSHAAAGPNLG
                     LNASANEAKQSYNYLFPKAYTPKNYQDYGQVTLNFGWDLDFWGKNRAAITAATNSARA
                     AEADAAQARLMLAAAVTSAYVDLDRLYQERDLAARAVDIHSDSASLVRQKLDNGVANR
                     ATLDQAQANVYAARADLAALDEQIDLGKHLIAALIGAAPDAAANIRRPQLKPADSAWT
                     APQKVDLDLIGRRPDIIAARWRAEAASAQIREAHAAFYPNINLAAFVGVQSLHLDQLL
                     QPGSDIGQAGPALSLPLFDGGALKANLKTAEAGHALAIASYNQDVLTAMQQVADALTS
                     QRALHDRLDNAQKAQDAYEEAYRLAKLRYDGGLSDYTSLLVAEQALINQRRAVADLKS
                     RALTLDVALIKALGGQYNA"
BASE COUNT         1035 a         1799 c         1567 g          785 t
ORIGIN      
        1 tgtgccatct gtcgggcgtg gccagcctga ccgcgcgata cgtcgccgcg ctggacgggc
       61 tgaacgcgcg catcgtctgc acgcgcaaga ccctgccctc cttgcgcgca ctgcaaaagc
      121 acgcggtgcg ctgcggcggc ggctacaatc accgctacgg cctcgacgac gccatcctga
      181 tcaaggacaa tcacatcgcc atctgcggcg gcgtcgaagc ggccatgcaa cgcgccaagg
      241 cgggcgtcgg ccacaccatg aagatcgaga tcgaggtcga ttccatcgcc cagctcaagc
      301 ttgccctgcc cttcaagccg gacgtcatcc tgctcgacaa cttcacgacc gacatgttcg
      361 cgcaggccgt gaagctggcg gcgggaaaga ccctgctcga agcgtccggc ggcgtcaatc
      421 tcgacaccgt gcgcgccatc gccgaaaccg gcgtcgacgt catcagcgtc ggcgccctga
      481 cccactcggc gccgaatttc gacatcggtc tggacgcggt ctgattgacc ggcggcgggc
      541 ttcattgtac aatttcgcgt acaaagaggc cccgccatga aacacgtcac ctattccgcc
      601 ttccgccaga atctggccaa gctgctcgat caggtcaatg acgatcacgc gccgctgctg
      661 gtgacgcgcc agaacggcgc gcccaccgtg gtcgtatcgc tggaggattt caccgcctgg
      721 caggaaaccc tgtacctgcg cgccagcccg aagaacgccg aacggctgag ccgatccatt
      781 gagcgtctga atgcgggatt gggtgaagcc cacgacctga tcgaagaatg aagatcatct
      841 tcgatccgga cgcctgggag gattatctct actggcaagg ccacgacaag atcacggtca
      901 agcgcatcaa cgccctgatc aaggacatag cgcgccatcc gtttgaaggc ataggccagc
      961 ccgaagcgtt gaaatatgac tggtcaggtt actggtcgcg ccgcatcgat caggaacatc
     1021 gtttcattta cagagttccg ccggacgcga tcctgatcgc gcaatgccgc taccattatt
     1081 gaccgtagcg gtccaaggat atacattatc gtcacgtata tccaaggagg ccgtcatgca
     1141 ggtcgcaaaa tggggcaatt cgctggccgt gcgcttaccg gccgccgtgg tcgaggcgct
     1201 ggagctgaaa gtgggcgacg acatcgccat tcgcgccgag agccgccgcg acttcgtgat
     1261 cgagcgccag cccacgcggg acgagcttat caagcggttg gatgctctac gtttccgcat
     1321 gcctgcggat tacaagttcg atcgagaaga ggcgaatgag cgttaagcgc ggctttattg
     1381 acaccaatgt gctggtttac gcactgtcgg aaggcaatga gaaagccgcc agagccgcgg
     1441 agttacgcaa tgccggcggc gttatcagcg ttcaggttct gaatgaactc gtgaacgtgc
     1501 tgcgccgcaa gcttaaacac ccgtggccgg aaacgctgca acggctgatg atcgtgaaat
     1561 cgctgcttga gatcgtcccc ctcacgctgg aaacccacga agccggactg gagttggcgc
     1621 gccggtacag tctttccctc tatgacgcca tgatcgtggc gtccgcactc gaagccgggt
     1681 gcgacgccct gtggtcggag gacatgcagg acggcctgcg catcggcgat ctgacgatcc
     1741 gcgatccgtt caggcgccgc ccttaacgaa gctcaaccgc gacacgcgct ggctcagcgg
     1801 cttgtcgacc agcgcgtcga agacgaaggc ggcgataacg gccagcagca cgccgaggct
     1861 ccataaggcc cactgcgcgg cggcgccgag gccgaaatgc gcggtcagtc tggccgtaac
     1921 gccgaaccag atcgcgccga ccagcgaatg cacgaggaac aaagcgaacg aggcgcgccc
     1981 catcagccgg gtcgccgtgg tttccgggaa ggtcaccccg cccgacaccg ccagcgccag
     2041 cgccagcagg cccagcagca gggtgtccat gccgatattg cgcggcagca ggcataaaaa
     2101 cggcagggcc agcagcaccc cgacgctgcc gatccagaag accggcttcg gcgtcgtcag
     2161 gccggaggtc gcccgttcga tcaggctgcc gatcacgaac agcggaatcg cccgccccag
     2221 tccccattcg aacggcaggt cggccagcgg atgacgcacg acgctgagcg cgaacaggtg
     2281 cgcgacggcc agcacgccca gcgccagcgc ggccagccgc cagcgcccca gccgccacat
     2341 ccacggcgcg tagaagctga acaggccgta gcagatcagc agcgccgaga tcgtccatgt
     2401 cggcacgttc cacgacggcg cggcgaacat gccccagcca tgcaccatga agacctgagc
     2461 gaggaaggcg aaggcgccgt acttgtccgg gtgtttgggc atcagcccca ccgcgccgga
     2521 cgccagcacg aaagccccga aggccagcag cacgatcaca tgcgacggcc acagacgcgc
     2581 gaaccggcgc aggaagaagt gcgagcgccg catcccggac tcgaccagcc gccgcccata
     2641 ggcgcgcgac agcacgtaac cggacagcat caggaagaaa tcggtcgcca gccagccttg
     2701 ccggaacacc ggcacgatcg tctccagatc gcgcggcgcg ttgctgccgt aatggaacag
     2761 aaggatgaac agcgccgcca gaaagcgcag cacgtccagc gaagcgccgc ccatacgcgc
     2821 ctggatcggc gtcccggcgg acccggttat ggaaaagttg agcggcttca cgccgcgcat
     2881 cctaatccgg cagcggttaa gattttgaag ccgcggcggc gttcgcgccg taggcggcga
     2941 aaaataccgc tatcgcgccc tcgacctcgg cttcgatttc ggcgtccgtc ggctccggca
     3001 tgtagttgag caggcgcgcc ttgatcatgc gcccgtggat cagcgacgtg aaatggtgcg
     3061 cggccgtcat cacgtcgggc gcgatgaggt cgccgcgatc gcgcgccttt tcgatatagg
     3121 cggcgatgat gcgcgcgccg gtcagcggcc cggcttcgta gaacatgcgg ccgacatccg
     3181 gcgacttatg gctttcggcc gagatcacgc gccagttctg caaggccggt tcggcggtga
     3241 aggcgcgcac atagcccctg gcgaaacggg tcagcacggt gcgcgcgtcg ccctcctcgg
     3301 tcaggaggtg cgcgatctcg ccgcgatgaa agacgcagga gcgctgcaca taggcctcga
     3361 acaactcttc cttgctcttg aaatagttat acagcgtccc cttcgagccg ccgaccctgg
     3421 ccgcgatggt ggacatggag gcgctggcgt agccctcctt gaggaaaaca tccgtcgcca
     3481 catccaaaat cgcctcgcgg cggtcttcgc ggtccatgcg ccccttgcat cccgcctcgc
     3541 ccccgctcgg cgtcatatcg cctgtgtgca ttgcaaaaaa ttcctcaacc gtacggtatg
     3601 gtcagcttga cagatactat gcaccggact atatgtccag caaaatgacc ataccgtacg
     3661 gttatgtgat gcgccgtacc gactgactcc agagtggcaa aatgacgcac ctccgccctt
     3721 ccgtccccct cgccgcggcg ctgctggccg caaccgccct gacggcctgc acgaccctgc
     3781 cgcagagcgc gccctacgcg accgagccga gccaggccaa gaaggtcaat ccgatcgcgg
     3841 cgatcaccga gcaggactgg tggaaaagct ttggcgaccc gcagctcgac ggcctgatga
     3901 cgcaggcgct caacaacgcg cccagcctcg ccatggccga ggcgcgcctg aacgcggcgc
     3961 aggccgtcgt atcgtcctca cacgccgccg ccgggccgaa tctcgggctg aacgccagcg
     4021 cgaacgaagc caagcagagc tacaactacc tgtttccgaa agcctacacg cccaagaact
     4081 atcaggatta cggtcaggtc acgctgaact tcggctggga tctcgacttc tggggcaaga
     4141 accgcgccgc catcaccgcc gcgaccaatt cggcgcgcgc cgccgaggcc gacgccgcgc
     4201 aggcgcggct gatgctggcc gccgccgtga cctcggccta tgtcgatctc gaccgcctct
     4261 atcaggagcg cgatctggcc gcgcgcgccg tcgacatcca cagtgacagc gccagtctgg
     4321 tgcggcaaaa gctcgacaac ggcgtcgcca accgcgccac gctggatcag gcgcaggcca
     4381 atgtgtacgc cgcgcgcgcc gatctggccg cgctggacga gcagatcgat ctgggcaagc
     4441 acctgatcgc cgccctgatc ggcgccgcgc ccgacgccgc cgcgaacatc cggcgcccgc
     4501 agctcaagcc cgccgacagc gcatggaccg cgccgcaaaa ggtcgatctc gacctgatcg
     4561 gccgccgccc ggacatcatc gccgcgcgct ggcgcgccga ggcggcgtcc gcgcagatcc
     4621 gcgaggccca cgccgccttc tatccgaaca tcaatctggc cgccttcgtc ggcgtgcagt
     4681 cgctgcacct cgaccagttg ctccagcccg gttcggacat cggtcaggcc ggtccggcgc
     4741 tcagcctgcc cctgttcgac ggcggcgcgc tcaaggccaa cctgaaaacc gccgaggccg
     4801 gtcacgccct cgccatcgcc agctacaatc aggacgtcct gaccgccatg cagcaggtcg
     4861 ccgacgccct gaccagccag cgcgccctgc acgaccgcct cgacaacgcg caaaaggcgc
     4921 aggacgccta tgaggaagcc tatcgtctgg ccaagctgcg ctatgacggc ggcctttccg
     4981 actacacctc gcttctggtc gccgaacagg cgctgatcaa ccagcgccgc gccgtcgccg
     5041 acctgaagag ccgcgccctg acgctggacg tcgccctgat caaggccctc ggcggccaat
     5101 acaacgctta aaacataacc tccgctctca gagattccgt catgtccgac cccgctcaag
     5161 ccaagccccc cgctcaagcc aacccc
//
LOCUS       DASVLL010000036         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_109539,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000036 DASVLL010000000
VERSION     DASVLL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109539"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VF402_00635"
     CDS             complement(<1..134)
                     /locus_tag="VF402_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798821.1"
                     /translation="MYQYDAFDHAFIRDRVADFSDQVARRLSGELTEDQFKPLRLMNG
                     L"
     gene            complement(136..453)
                     /locus_tag="VF402_00640"
     CDS             complement(136..453)
                     /locus_tag="VF402_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEX7798822.1"
                     /translation="MKLLTANRLSDGVTIWYAAEGWPQGWVEDAAHAARLDDAEAEAL
                     QKAWAQRETEVVAPYLVPLRDNGSPVQREHVREFVRANGPTTGPTRDALARAFARQDV
                     FED"
     gene            complement(450..1859)
                     /gene="cysG"
                     /locus_tag="VF402_00645"
     CDS             complement(450..1859)
                     /gene="cysG"
                     /locus_tag="VF402_00645"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEX7798823.1"
                     /translation="MLALPLSWPLKDKRVALIGDGAWLTRKLTLLRRTPAQLSVYAPF
                     GHVTSLAGETVEQRWPEASELSGAALIVVAFEDRALAERGAALARQTRAPLNVVDYPD
                     LSDFHFPAIIDRGALSIGVATGGTAPVLAREARRRIEAAVPPSETHIAAFAEALSAPL
                     RERFSDVDDRRRVWEQVLASPAAETARAGRVDEAVEQALQALENHAPRKGVVHLVGAG
                     PGDPELLTLKALRLLSEADVIVYDRLVGDGIMDLARRDADRFYVGKQRSNHSVPQAQI
                     HDLLLEQARLGKRVVRLKGGDPFVFGRGGEEVEALRAAGIEVHIVPGITAALGCAASS
                     GVPLTHRDYAQSVTFITGHSRDGDHSNPLMLDWSALSAPNHTLVVYMGVATAREIAGR
                     LMRHGRASDTPVLIIENGTRPDERRTPATLATLEAAVAAVTGPALLIVGEVAALYTSD
                     DAAAGVIEQAQKTIMGMNA"
     gene            complement(1993..2340)
                     /locus_tag="VF402_00650"
     CDS             complement(1993..2340)
                     /locus_tag="VF402_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7798824.1"
                     /translation="MRHTGRCHCGAIAFEAEGAFDSGLSCNCSICRRKGSLLAFIPAS
                     DFTLTTPKDKVATYTFNRRVIRHHFCTACGVSPYAEAPAPDGTPTVALNLRCLPDVDL
                     DALTITHWDGASH"
     gene            2418..2855
                     /locus_tag="VF402_00655"
     CDS             2418..2855
                     /locus_tag="VF402_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEX7798825.1"
                     /translation="MSSEHALEARRKIREDAAAIGVDEAFISRLVDEFYRRVRLHPHL
                     GPVFNGAVEDWPEHLAKLKLFWQSVALNAGVYTGRPMQAHMRVPSIEPAHFGEWLHLF
                     EQTLRDLAEDERAVEYFMMRANRIGESLKLGLFYRPEQDLRGA"
BASE COUNT          464 a          894 c         1027 g          483 t
ORIGIN      
        1 aggccgttca tcagccgcag cggtttgaac tgatcctcgg tgagctcgcc ggacaggcgg
       61 cgcgcgacct gatcggaaaa gtcggcgaca cgatcgcgga tgaaggcgtg gtcgaaggcg
      121 tcgtactgat acatcttaat cctcgaaaac gtcctgacgg gcgaaggcgc gggccagcgc
      181 gtcacgggtc gggccggtgg tcgggccgtt ggcgcgcacg aattcgcgca catgttcgcg
      241 ctgcaccggc gaaccgttgt ccctgagcgg gaccagatag ggcgcgacca cctcggtttc
      301 gcgctgcgcc catgcttttt gcagcgcctc ggcctcggcg tcgtcaaggc gcgcggcgtg
      361 ggccgcgtcc tccacccagc cttggggcca gccctcggcg gcgtaccaga tcgtcacgcc
      421 gtcgctgaga cggttggcgg tcaggagctt catgcgttca tccccatgat agttttttgg
      481 gcttgttcta tgacgcccgc ggcggcgtcg tcggaggtgt agagcgcggc gacctcgccg
      541 acgatcagca gggccgggcc tgtcacggcg gcgacggcgg cttccagcgt ggccagcgtg
      601 gcgggggtgc ggcgctcgtc cgggcgcgtg ccgttctcga tgatcagcac gggcgtgtcg
      661 gacgcgcggc cgtggcgcat caggcgcccg gcgatctcgc gcgcggtggc gacgcccata
      721 tagacgacca gcgtatggtt cggcgccgac agggcggacc agtccagcat cagcgggttg
      781 gaatggtcgc cgtccctgga atggccggtg atgaaggtca cgctctgggc gtaatcgcgg
      841 tgggtcagcg gcacgccgga cgaggccgcg cagccgagcg ccgccgtaat gccgggcacg
      901 atatggacct cgatcccggc ggcgcgcagg gcttcgacct cctcgccgcc gcgtccgaag
      961 acgaacgggt cgccgccttt caagcgcacg acgcgcttgc ccaggcgggc ctgctccagc
     1021 agcaggtcgt ggatctgcgc ctgcggcacg gaatggttcg agcgctgctt gccgacatag
     1081 aagcggtcgg cgtcgcggcg ggccaggtcc atgatcccgt cgccgacgag gcggtcatag
     1141 acgatgacgt cggcctcgga cagaaggcgc agcgccttga gcgtcagcag ttcgggatcg
     1201 ccgggaccgg cgccgacaag atgcacgacg cctttgcggg gcgcatggtt ttcgagcgct
     1261 tgcagcgcct gttccacggc ttcgtcaaca cggcctgcgc gcgccgtctc ggcggcgggg
     1321 gaggccagaa cctgctccca gacgcggcgg cggtcgtcga catcgctgaa ccgttcgcgc
     1381 aaaggcgcgc tcaaggcttc ggcgaacgcg gcgatatggg tttcggacgg cggtacggcg
     1441 gcctcgatgc ggcggcgcgc ctcgcgcgcc agcaccggcg cggttccgcc cgtggcgacg
     1501 ccgatgctca gggcgccgcg atcgatgatg gccgggaaat ggaagtcgct gaggtcggga
     1561 tagtcgacca cgttcagcgg cgcgcgggtc tgacgggcga gggccgcgcc gcgctcggcc
     1621 agcgcgcgat cctcgaacgc caccacgatc agggcggcgc cggataattc ggaagcttcc
     1681 ggccagcgct gttcgacggt ttcgcccgcc aaagacgtca catgcccgaa tggcgcatag
     1741 acgctgagct gcgccggggt gcggcgcagc agggtcagct tgcgcgtcag ccacgcgccg
     1801 tcgccgatca gcgcgacgcg cttatccttc aacggccagg acaggggcag ggccagcatg
     1861 ggcggcgaac tccggggaac cgagttcgcc tttctagtct ctcgcgcctg cgaagaaaag
     1921 cgatggtttg tcgtgagagc gtttagatca actgactcta tccaaaaagc gtcatccttt
     1981 ttgggatgcg ctctaatgac tggcgccgtc ccaatgggtg atggtgagcg catcgagatc
     2041 gacatcgggc aggcagcgca ggttaagcgc cacggtcggc gttccgtccg gcgccggggc
     2101 ctcggcatag ggcgacacgc cgcaggccgt gcagaaatgg tggcggatga cgcgcctgtt
     2161 gaaggtgtag gtcgcgacct tgtccttcgg cgtggtcagg gtgaagtcgc tggccgggat
     2221 aaaggccagc agcgagcctt tgcggcggca gatcgagcag ttgcagctca ggcccgagtc
     2281 gaacgcgccc tcggcctcga aggcgatggc gccgcagtgg catcttccgg tatggcgcat
     2341 ggtttttctc cgttttcgcc gccctatgac aaggcgccgc atggtcaaat tgcgtcagca
     2401 gggctagaat gtcgtccatg agttccgaac acgcgcttga agcccgccgt aaaatccgcg
     2461 aggacgccgc cgctatcggc gtcgacgaag cctttatctc caggctggtc gatgagtttt
     2521 accggcgggt gcggctgcat ccgcatctgg ggccggtgtt caacggcgcg gtcgaggact
     2581 ggccggagca tctggccaag ctcaagctct tctggcagtc ggtggcgctg aacgccgggg
     2641 tctataccgg acggccgatg caggcgcata tgcgggttcc gagcatcgaa ccggcgcatt
     2701 tcggcgaatg gctgcacctg ttcgagcaga ccttacgcga tctggccgag gacgagcgcg
     2761 cggtcgaata tttcatgatg cgcgccaacc ggatcgggga gagcctgaaa ctgggcctgt
     2821 tctaccgccc ggagcaggat ttgcgcgggg cgtgaaaata gactaagc
//
LOCUS       DASVLL010000037         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_216852,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000037 DASVLL010000000
VERSION     DASVLL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216852"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..564)
                     /locus_tag="VF402_00660"
     CDS             complement(58..564)
                     /locus_tag="VF402_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798826.1"
                     /translation="MPRLSFAALAFAALGLAPLMAVAQDYGDGNAPGDRLTPYDRAHD
                     DDDYARQGDSYDGSGVPYAGRYLGYDYVAGKYYRWDSPEPQDRRAREQDGWRDGVTHT
                     RPVGYGWRRYDLCGCGRYSYYRTYGRWGQRGSTQLDPRRLDYTPSYAYAPRAYYRSWI
                     TRRSDFPW"
     gene            729..1967
                     /locus_tag="VF402_00665"
     CDS             729..1967
                     /locus_tag="VF402_00665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX7798827.1"
                     /translation="MDSFDLDIARVGFQGDGLSADGHIVPLTLPGERVRVREGKGQAE
                     LLDILTPSPRRVTPPCPHYAACGGCALQHWDMAAYSDWKREVVIAHLRRAGLEGEVAP
                     IFTVPPHSRRRVGLHARKTGKSVLLGFKARRSWTLVPIETCVIAEPAIVAALPHLRDL
                     AAPLFEHPKSAPILHVTASLTGLDIDISGVERSKSGGLSADARMRIAMTAAEADLARV
                     TMGDDILYQARPAMIAFGRARAALPAGSFLQASREAEVEMARLVTEGIAGAKRVADLF
                     CGAGAFSFPLAEQATVLALDGAAPAIAALRTAIASAPGLKTITAEARDLFRRPLLAEE
                     MKGLDAVVFDPPRAGAEAQSREIARSNLSRAVAVSCNPATFARDAKILTEAGFRLDSV
                     TPIDQFLWSAHVELVAVFSR"
     gene            1992..2648
                     /locus_tag="VF402_00670"
     CDS             1992..2648
                     /locus_tag="VF402_00670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7798828.1"
                     /translation="MPTLNAEDTLARTLSALVPGVVEGLIKEVIVVDGGSQDATLEIA
                     DSTGCVIVHGDAERGLQLWQGCRAARGDWLMILHQDSTLGEGWMDQAQAHIRDYPLRA
                     GYFHLKFDDPSWPVALWAEMLALRARWLAMPSGDHGLLLSRALYDCTGGYKDQAAFED
                     VALSMALGRARLRPMSAALITSAARFHERGWAFGLLGKSFRFVLYLLGIPPKPPVRKA
                     "
     gene            2743..>3188
                     /locus_tag="VF402_00675"
     CDS             2743..>3188
                     /locus_tag="VF402_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7798829.1"
                     /translation="MNRIAVVAAAGDIVDREGFGNLTMAGLAKRLGVALPSLYTHVRS
                     LDHLRRELALVVTEELSRRMGEAIQGRAGRDAVFALAGAYRDYVLRCPGRYTMAMAAQ
                     PAPSEDDARYRDAALKCAGVVYSAVRGYGVAEPALTDAARFLRAAL"
BASE COUNT          490 a         1039 c         1122 g          537 t
ORIGIN      
        1 aaaaaagacc gcccttttga gggggcggtc aggcgctcgg ggggaggaag ggtacgctta
       61 ccagggaaaa tcgctgcgcc gggtgatcca gcttcgatag taggcgcgcg gcgcgtaggc
      121 atagcttggc gtatagtcga gacggcgcgg gtcgagctgc gtagagccgc gctggcccca
      181 gcggccatag gtgcggtagt aactgtaacg tccgcaaccg cacagatcgt agcggcgcca
      241 gccgtaaccg accgggcgcg tatgggtcac gccgtcgcgc cagccgtcct gttcgcgcgc
      301 gcgccggtcc tgcggctccg ggctgtccca gcggtaatac ttgccggcga cgtagtcgta
      361 gccgagataa cgcccggcgt aaggcacgcc gctgccgtcg tagctgtcgc cctgacgggc
      421 gtaatcgtcg tcgtcatgcg cgcggtcgta aggcgtgagg cggtcgcccg gagcgttgcc
      481 gtcgccgtaa tcctgagcca cggccatcaa cggcgcaagg cccagggcgg cgaaggctaa
      541 ggcggcgaag ctcaggcgcg gcattgcggg catccccctt tggttgattc cagtatcgct
      601 gaaaaattga aaaacgggaa tagggcaatg tggtattttt ataagatttt catcaagacg
      661 tcgcgatgcg ttaagttgcg gtttgcgctc cgatgcgtta agcgggcggc ttttccacat
      721 ggggcctcat ggacagcttc gacctcgaca tcgcgcgcgt cggttttcag ggcgacggcc
      781 tgagcgcgga cggccatatc gtcccgctga ccctgccggg cgagcgcgtg cgcgtgcgcg
      841 aaggcaaggg gcaggcggaa ctgctcgaca tcctgacgcc gtcgccgcgg cgcgtgacgc
      901 ccccatgccc gcactatgcg gcctgcggcg gctgcgcgct gcaacactgg gacatggccg
      961 cctacagcga ctggaagcgc gaggtcgtga tcgcgcacct gcgccgcgcg gggctggaag
     1021 gcgaggtcgc gccgatcttc accgtcccgc cgcacagtcg ccgccgcgtc gggctgcatg
     1081 cgcgcaagac gggcaagagc gtgctgctcg gcttcaaggc gcggcggtca tggacgctcg
     1141 ttcccatcga aacctgcgtg atcgccgaac cggccatcgt cgccgccctg ccgcatctgc
     1201 gcgacctggc cgcgcctttg ttcgagcatc cgaagtccgc gccgatcctg cacgtcaccg
     1261 catcgctgac cggcctcgac atcgacattt ccggcgtcga gcgcagcaaa agcggcggcc
     1321 tgtcggcgga cgcgcgcatg cgcatcgcca tgaccgccgc cgaggccgat ctggcgcgcg
     1381 tcacgatggg cgacgacatc ctctatcagg cgcgcccggc catgatcgcc ttcggcaggg
     1441 cgcgcgcagc cttgcccgcc gggtcgttcc tgcaagccag ccgcgaggcg gaagtcgaga
     1501 tggcgcggct ggtcacggaa gggatcgccg gggcgaagcg cgtggccgac ctgttctgcg
     1561 gcgccggggc cttcagcttt ccgctggccg aacaggcgac ggtgctggcg ctggacggcg
     1621 ccgcgcccgc catcgccgcg ctcaggaccg ccatcgccag cgcgccgggc ctcaagacga
     1681 tcacggcgga ggcgcgcgac ctgttccgcc gcccgctgct ggccgaggag atgaaggggc
     1741 tggacgccgt cgtcttcgat ccgccgcgtg cgggcgccga ggcgcagagc cgcgagatcg
     1801 cgcgctcgaa cctgtcgcgc gccgtggcgg tcagttgcaa tccggccacc ttcgcgcgcg
     1861 acgccaaaat tctgaccgag gccggattta ggctggacag cgtcacgccc atagaccagt
     1921 tcttgtggtc cgcccatgtc gagcttgtgg cggtcttttc ccggtaaagg ttcccgatga
     1981 tttccgtcgt catgcccacg ctcaacgccg aagacacgct ggcgcgcacc ttgtcggcgc
     2041 tggtgcccgg cgtggtcgag gggctgatca aggaggtgat cgtggtcgac ggcggctcgc
     2101 aggacgcgac gctggagatc gcggattcga ccggctgcgt catcgtccac ggcgacgccg
     2161 agcgcggcct gcaactgtgg cagggctgcc gggcggcgcg cggcgattgg ctgatgatcc
     2221 tgcatcagga ttcgaccctg ggcgagggct ggatggatca ggcgcaggcc catatccgcg
     2281 actatccgct gcgggccggg tattttcacc tgaaattcga cgatccgtcc tggcccgtgg
     2341 cgctgtgggc cgagatgctg gccctgcgcg cgcgctggct ggccatgccc agcggcgacc
     2401 acggcctcct gttgtcgcgc gcgctgtacg actgcacggg cggctataag gatcaggcgg
     2461 cgttcgagga cgtggccctg tcgatggcgc tgggccgtgc gcgcctgcgc ccgatgtcgg
     2521 cggccctgat caccagcgcc gcccgcttcc acgagcgcgg ctgggcgttc ggcctgctgg
     2581 gcaagagctt ccgcttcgtg ctgtacctgc tcggcatccc gcccaagccg ccggtgcgga
     2641 aagcctgaag cctgcgcgga ttgtcttgac tcgaatgtga ttccgatatt tcctaatatc
     2701 agtaggaaat atcggaattt atataatatg ggcaggccgg gtttgaatcg catcgccgtc
     2761 gttgcagcgg ctggcgacat cgtcgacagg gaaggcttcg gcaatctgac gatggcggga
     2821 ctggccaagc gtctgggggt ggccttgccc agcctttaca cgcatgtgcg cagcctcgac
     2881 cacctgcgcc gggaactcgc gctggtcgtc acggaggaac tgtcccggcg catgggcgag
     2941 gcgatacagg gacgcgcggg gcgcgacgcg gttttcgcgc tggcgggcgc ttaccgggat
     3001 tatgttctgc gatgccccgg acgctacacc atggcgatgg cggcgcaacc ggcgccatcg
     3061 gaagacgatg cgcggtatcg cgacgcggcc ctgaaatgcg cgggcgtcgt ttacagcgcc
     3121 gtccggggct atggcgtggc cgaaccggcc ctgaccgacg ccgcgcgttt cctgcgcgcc
     3181 gcgctgca
//
LOCUS       DASVLL010000038         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_324367,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000038 DASVLL010000000
VERSION     DASVLL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324367"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VF402_00680"
     CDS             <1..1071
                     /locus_tag="VF402_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7798830.1"
                     /translation="AQAWQVTWMFTAYSLGQFFAEPFCGRLSDRIGRKPILIATTALS
                     VVFYVLLAFSPNIWAAILVRFLCGLASGNISTIQGYVSDFSKPEQRASRMSLIGAAFS
                     LGFIVGPVIGGLLTHEHFGGNAFRPPLFAAAVLSGLATLGVVLFVRESRQRTQHSAPP
                     QNMLKTAQEALKSRVIVRVILATLCYMMAFAGLESTFGLWVEVRYNWHAAQIGAVFLF
                     IGATAAVMQMVVMRPLVRRWGESKVLAAGLFVFGISFILQSVNHVSWLIVPLLMLGTA
                     GQAVIFATICAIISMATTPERQGAMLGLNMSTGAVARILGPMAAGFLFSQFGPEAPLW
                     LDAAMTLPAALLALQVGKVQKR"
     gene            1068..>1701
                     /locus_tag="VF402_00685"
     CDS             1068..>1701
                     /locus_tag="VF402_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798831.1"
                     /translation="MTLIDPFETDWRRFHPGRFPLGHMLRHAEGWNGTRFHLLPDNRA
                     AVANRAEFRALIERYNTIGTATLGEDAECWLALVQSPNQDAKSRARMNRIKKRYDMQA
                     GWQFFSATDNLSYTVWTAKVDWRENAFNRLFIQTYHQDVWDVLWMRVADGAVFYPYGS
                     GVDVAQPTPQALIDLVSRYYGWLPVPGAGGYLKFNPKQMAGVRFQVSKPCA"
BASE COUNT          248 a          565 c          568 g          320 t
ORIGIN      
        1 gcgcaggcgt ggcaggtgac gtggatgttc accgcctaca gtctgggcca gttcttcgcc
       61 gagccgttct gcggccgcct gtccgaccgc atcggacgca agccgatcct gatcgccacc
      121 accgccctgt cggtggtttt ctatgtcctg ctggcgtttt cgccgaacat ctgggcggcg
      181 atcctcgtgc gcttcctgtg cgggctggcc tccggcaaca tctcgaccat tcagggctat
      241 gtctcggatt tttccaaacc ggaacagcgc gccagccgca tgagcctgat cggcgccgcc
      301 ttttcgctgg gcttcatcgt cgggccggtg atcggcggcc ttctgacgca tgagcatttc
      361 ggcggcaacg ccttccgccc gcccctgttc gccgccgccg tcctgtcggg tctggcgacg
      421 ctgggcgtgg tcctgttcgt gcgcgaaagc cgccagcgca cgcagcacag cgcgccgccg
      481 cagaacatgc tgaaaaccgc gcaggaggcg ctgaaaagcc gcgtcatcgt gcgcgtgatc
      541 ctggcgaccc tgtgctatat gatggcgttc gcggggctgg aatcgacctt cgggctgtgg
      601 gtcgaggtgc gctacaactg gcacgcggcg cagatcggcg cggtcttcct gttcatcggc
      661 gccaccgccg ccgtcatgca gatggtggtc atgcggccgc tggtgcggcg ctggggcgaa
      721 tcgaaggtgc tggccgccgg gctgttcgtg ttcgggatca gcttcatcct gcaaagcgtc
      781 aaccacgtct cgtggctgat cgtgccgctg ctgatgctgg gcacggcggg gcaggcggtg
      841 atcttcgcca ccatctgcgc gatcatctcg atggccacga cgccggagcg gcagggcgcc
      901 atgctgggcc tgaacatgtc gaccggcgcg gtggcgcgta ttctcggccc gatggcggcg
      961 ggcttcctgt tctcgcaatt cggccccgaa gcgcccctgt ggctcgacgc ggccatgacg
     1021 ctgcccgcgg cgctgctggc gctgcaggtc ggcaaggtgc agaaacgatg accctgatcg
     1081 acccgttcga gaccgactgg cggcgcttcc atccggggcg ctttccgctc ggccacatgc
     1141 tgcgccacgc cgagggctgg aatgggacgc gctttcactt gctgcccgac aaccgcgccg
     1201 ccgtcgccaa ccgcgccgag ttccgggcgc tgatcgagcg ctacaacacg ataggaaccg
     1261 cgacgctggg cgaggacgcc gagtgctggc tggcgctggt gcagtcgccc aatcaggacg
     1321 cgaagtcgcg cgcccgcatg aaccgcatca agaagcgcta tgacatgcag gcgggctggc
     1381 agttcttttc cgccaccgac aatctcagct atacggtctg gacggcgaag gtcgactggc
     1441 gcgaaaacgc cttcaaccgc ctgttcatcc agacctatca tcaggacgtc tgggacgtcc
     1501 tgtggatgcg cgtcgcggac ggcgcggtct tttaccccta cggctccggc gtcgatgtcg
     1561 cgcagccgac gccgcaggcg ctgatcgatc tggtgtcgcg ctattacggc tggctgccgg
     1621 ttcccggcgc gggtggctat ctgaagttca atccgaagca gatggcgggc gtgcgctttc
     1681 aggtcagcaa gccctgcgcc g
//
LOCUS       DASVLL010000039         4310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_163520,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000039 DASVLL010000000
VERSION     DASVLL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163520"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VF402_00690"
     CDS             complement(<1..661)
                     /locus_tag="VF402_00690"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEX7798832.1"
                     /translation="MSAYRNLREFIARLEAEGELVRVKTPVSTHLEMTEIQTRLLAEK
                     GPAVLFENVLLPDGSPSGMPVLVNLFGTVKRVAMGVTLERTPKSGGHFSEEGARSNKK
                     SGKDRATGEELREVGELLAFLRQPEPPRGFKDALELLPLAKTVMGMRPKTVKSAPVQE
                     VVLTGDRIDLTKLPVQGCWPGEPAPLITWPLIVTKGPSDAREDDYNLGIYRMQVLSKN
                     RT"
     gene            719..1585
                     /locus_tag="VF402_00695"
     CDS             719..1585
                     /locus_tag="VF402_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7798833.1"
                     /translation="MENVLIIGARTLVGRRLRAALLAAGWPEAAVWHASRRQTGGQGL
                     VLDMARPDAFAPERAFTHVIVCAPVWLIGDALIRRLAGQGMRRLIAFSSTSRLTKGES
                     DEAAERDVVAALIAGEATVARACDDVGVAWTILRPTLIYDEGRDENVSRIARVIRKLG
                     VFPLYGAATGLRQPVHARDLAAAAAAALRAEASYGKTYNLPGGETLAYRDMVKRICAA
                     LRRPALLLPAPEGLWRSGFAVLSALRGRPLSNNLQMARRMNVDLCFDGGEAARDFGYK
                     PGPFAPDFRRKD"
     gene            complement(1582..2811)
                     /locus_tag="VF402_00700"
     CDS             complement(1582..2811)
                     /locus_tag="VF402_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7798834.1"
                     /translation="MRTQLRARLAGLSLALLLAPVAALADPPPQTPPTPKQQLADLNA
                     KAEGGDAQANYDLAQVYVDGMYGQKKNEQTAAQWVQKAAQLGPADAQADYAHRLEIGK
                     GVKADPAAALDWYVKAADQGQARAVLRVCESFTQGPDADWSRAYPYCDKAADAGAATA
                     LYARGIALTQGKGAAADPAKGLKDLDAAADKDDGAALDALGVIYADGHLVPQDYRQAL
                     TYFRKAAARGDRDGVLRMAQQIETGQGAAADLNEAARLYDTLVRADPNDASGKAAKAW
                     LAAHPRIRTAAFADNILKVSDIARDTIFYAVDNDDPRFQTLDMAGYFDYLSRNSYPGE
                     AQNDGVSGEAIAECRFTATGDFDDCVLMRDAPPGHGFGAALMSIFNRLGQSGNKTDWA
                     KRYQGKVLRLSMRWKIN"
     gene            complement(2816..3160)
                     /locus_tag="VF402_00705"
     CDS             complement(2816..3160)
                     /locus_tag="VF402_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7798835.1"
                     /translation="MSKTHHTIDYIELPVDATGAAKAFYAAAFGWTFQDYGPTYAALS
                     AGIDGGFQADPAEKTPAPLAIVYSADLDASLAAVRLAGGEITRGVFDFPGGRRFHFRD
                     PSGNELAVWSDR"
     gene            complement(3135..3833)
                     /locus_tag="VF402_00710"
     CDS             complement(3135..3833)
                     /locus_tag="VF402_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7798836.1"
                     /translation="MDAAAPDLAARMDFIRETTRPIDVAGVPGVRLYQADEVTPLWLM
                     TEETMASRQLAPPFWAFAWAGGQALAKYLLDHPETVRGKRVLDIACGSGLVGVAAMKA
                     GAARVVCNDIDPYAEAAVGLNARLNGVDIAFQSGDLLDGPAPDADLVMAGDVTYEKAM
                     SAAMLSYFRRAARDKIEVLIGDPHRGYFPRDAGLKKLADYIISTHADIEDAREKPASV
                     WKLEADHEQDAPHH"
     gene            complement(3833..>4310)
                     /locus_tag="VF402_00715"
     CDS             complement(3833..>4310)
                     /locus_tag="VF402_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HEX7798837.1"
                     /translation="VSLYAEEDDYQGGAHPNSAFQALLWDKTKNELIPTASLFAPGAD
                     MSGVNAFLCRQIEAARAQRLGAPVTQAASGFPCPALKDSRLILIPSTLDGKIGAIDAL
                     YAPYEVGAYAEGPYEARIPQSQLKGLIAPAYADQFAGEAIKQEALPDPDSDKDKAQ"
BASE COUNT          612 a         1463 c         1492 g          743 t
ORIGIN      
        1 tggtgcggtt cttactcaaa acctgcatcc ggtagatgcc gagattgtaa tcgtcctcgc
       61 gcgcatcgga cggccccttg gtcacgatca gcggccaggt gatcaacggc gccggttcgc
      121 cgggccagca gccctgcacc ggcagcttgg tcaggtcgat ccgatcgccg gtcagcacca
      181 cctcctgcac cggcgcggac ttcaccgtct tggggcgcat ccccatcacg gtcttcgcca
      241 gcggcaaaag ctccagcgcg tccttgaagc cgcgcggcgg ctccggctgg cgcaaaaagg
      301 ccagaagctc gccgacctcg cgcagttcct cgcccgtggc gcggtccttg cccgattttt
      361 tgtttgatcg cgcaccttct tccgaaaagt ggccaccact tttcggggtg cgctcgagcg
      421 tgacgcccat cgccacgcgc ttgaccgtgc cgaacaggtt gaccagcacc ggcatgccgg
      481 agggggagcc gtcgggcaga agcacgttct cgaacagcac ggcggggcct ttttcggcca
      541 gcagccgcgt ctggatctcg gtcatctcca gatgggtcga gacgggcgtt ttcacccgca
      601 ccagttcgcc ctcggcttcg agtctggcga taaattcccg caggttcctg tacgcgctca
      661 tgacggcctc attgaagtaa cggcttgctg aataggccgc cgcgggcaat atgaccagat
      721 ggaaaacgta ttgatcatcg gcgcgcgcac cctggtcgga cgaaggctgc gcgcggcgct
      781 gctggcggcg ggctggccgg aagcggcggt ctggcatgcc tcgcgccgcc agaccggcgg
      841 gcaggggctg gttctggaca tggcgcggcc cgacgccttc gcgccggagc gcgccttcac
      901 ccacgttatc gtctgcgcgc cggtctggct gatcggagac gcgctgatcc ggcggctggc
      961 cgggcagggc atgcggcggc tgatcgcttt ttcgtcgacg tcgcgcctga ccaagggcga
     1021 atcggacgag gcggcggagc gcgacgtcgt ggcggcgctg atcgcgggcg aggcgacggt
     1081 ggcgcgggcc tgcgacgacg tgggcgtggc gtggacgatc ctgcgcccga ccctgatcta
     1141 tgacgagggg cgcgacgaaa acgtcagccg catcgcgcgc gtgatccgca agctgggcgt
     1201 gtttccgctg tacggcgccg ccacgggcct gcgccagccg gtgcatgcgc gcgatctggc
     1261 cgccgccgcc gccgccgcgc tcagggccga ggccagttac ggcaagacct acaacctgcc
     1321 cggcggcgag actctggcct atcgcgacat ggtgaagcgc atctgcgcgg cgctgcgccg
     1381 gcccgccctg ctgctgcccg cgccggaagg gctgtggcgt tccggcttcg cggtcctgtc
     1441 cgccctgcgc gggcgcccgc tcagcaacaa cctgcaaatg gcgcggcgca tgaacgtcga
     1501 cctgtgcttc gacggcggcg aggccgcgcg cgatttcggc tacaaacccg gcccgttcgc
     1561 gccggatttc cgccggaaag actagttgat cttccagcgc atcgacaggc gcagcacctt
     1621 gccctgatag cgtttggccc agtcggtctt gttgccggac tggcccagcc ggttgaagat
     1681 gctcatcagc gccgcgccga agccgtggcc cggcggggcg tcgcgcatca gcacgcagtc
     1741 gtcgaaatcg cccgtcgcgg tgaagcggca ctcggcgatg gcctcgccgc tgacgccgtc
     1801 gttctgggcc tcgcccggat agctgttccg gctcagatag tcgaaatacc cggccatatc
     1861 gagcgtctgg aagcgcggat cgtcgttgtc gacggcgtag aagatggtgt cgcgcgcgat
     1921 gtcgctgacc ttgaggatat tgtccgcgaa ggcggcggtc ctgatcctgg ggtgcgcggc
     1981 cagccacgcc ttcgccgcct tacccgaagc gtcgttcggg tcggcgcgga ccagcgtgtc
     2041 gtagaggcgc gcggcttcgt tcaggtcggc ggcggcgccc tgaccggttt cgatctgttg
     2101 cgccatgcgc agcacgccgt cgcggtcgcc gcgcgcggcg gccttgcgga aataggtgag
     2161 cgcctgccgg taatcctgcg ggaccagatg gccgtcggcg tagatgacgc ccagcgcgtc
     2221 gagcgcggcg ccgtcgtcct tgtcggcggc ggcgtcgagg tctttcaggc ccttcgccgg
     2281 atcggcggcg gcgcccttgc cctgcgtcag cgcgatcccg cgcgcataga gcgcggtggc
     2341 cgcgcccgcg tcggcggctt tgtcgcaata gggatacgcg cggctccagt cggcgtccgg
     2401 cccttgcgtg aagctttcgc acacgcgcag gaccgcgcgc gcctgcccct gatcggcggc
     2461 cttgacgtac cagtccagcg cggcggcggg gtcggccttc acgcccttgc cgatctccag
     2521 ccggtgcgcg tagtcggcct gcgcgtcggc cggaccgagc tgcgccgcct tctgcaccca
     2581 ctgcgcggcg gtctgttcgt tcttcttctg gccgtacatg ccgtcgacat agacctgcgc
     2641 caggtcgtaa ttcgcctgcg cgtcgccgcc ttcggccttc gcgttcagat cggccagttg
     2701 ctgcttgggc gtcggcggcg tctgcggcgg cggatcggcc agcgccgcga cgggcgccag
     2761 cagcagcgcg aggctcaggc ccgccagtct tgccctcagt tgtgttctca tgcgcttacc
     2821 ggtccgacca gaccgccagc tcgttgccgg acggatcgcg gaaatggaag cggcgcccgc
     2881 ccggaaagtc gaagacgccg cgcgtgatct cgccgcccgc cagccggacc gcggcgagcg
     2941 aggcgtcgag atcggcgctg taaacgatcg ccagcggcgc cggtgtcttt tccgccggat
     3001 cggcctgaaa gccgccgtcg attccggcgc tcagggcggc gtaggtcggg ccgtaatcct
     3061 gaaacgtcca gccgaaggcg gcggcgtaga acgccttggc ggcgccggtc gcgtccaccg
     3121 gaagctcgat ataatcaatg gtgtggtgcg tcttgctcat gatcggcttc cagtttccag
     3181 acgctggccg gtttttcgcg cgcgtcctcg atgtcggcat gggtcgatat aatgtagtcg
     3241 gcgagctttt tcagcccggc gtccctggga aaatagccgc gatgcggatc gccgatcagg
     3301 acctctatct tgtcgcgtgc ggcgcggcga aaatatgaca gcatggcggc gctcatcgcc
     3361 ttttcatagg tgacgtcgcc ggccatgacg aggtcggcgt cgggcgcggg gccgtcgaga
     3421 agatcgccgc tctgaaaggc gatgtccacc ccgttcaacc gcgcattgag tccgacggcg
     3481 gcctcggcgt aaggatcgat gtcgttgcac accacgcgcg ccgcgcccgc cttcatggcc
     3541 gcgacgccga cgaggccgga gccgcaggcg atgtccagca cgcgcttgcc gcgcacggtt
     3601 tcggggtggt ccagcaggta tttcgccagc gcctgaccgc ccgcccacgc aaaggcccag
     3661 aagggcggcg ccaattggcg gctcgccatc gtttcctcgg tcatcagcca caaaggcgtg
     3721 acctcgtcgg cctgatacag gcgcacgccc ggcacgccgg cgacgtcgat cgggcgcgtc
     3781 gtctcgcgga tgaagtccat ccgcgccgcc agatccggcg cagccgcgtc cattactgcg
     3841 ccttgtcctt gtcggaatcg gggtcgggca gggcttcctg cttgatcgcc tcgcccgcga
     3901 actggtccgc ataggcgggc gcgatcaggc ccttgagctg gctttgcggg atgcgcgcct
     3961 catacggccc ctcggcgtag gcgccgacct cgtaaggcgc gtaaagcgcg tcgatcgcgc
     4021 cgatcttgcc gtccagcgtc gacgggatca ggatcaggcg gctgtccttg agggcggggc
     4081 agggaaagcc ggacgcggcc tgcgtgaccg gggcgccgag gcgctgcgcg cgcgccgctt
     4141 cgatctggcg gcacagaaag gcgttgacgc ccgacatatc ggcgcccggc gcgaacaggc
     4201 tggccgtggg gatcagctcg ttcttcgtct tgtcccacag cagggcctga aaggccgaat
     4261 tgggatgcgc gccgccctga taatcgtcct cctcggcgta gagcgagacg
//
LOCUS       DASVLL010000040         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_163569,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000040 DASVLL010000000
VERSION     DASVLL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163569"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VF402_00720"
     CDS             <1..675
                     /locus_tag="VF402_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7798838.1"
                     /translation="IVLPHGGPRARDYIEFDWQAQCLAANGYAVLQPNYRGSTGYGQA
                     FLDAGNGQWGRKMQTDLSDGVRYLAARGLVDPKRVAILGASYGGYAALAGATLDPGVY
                     NCAVSIAGPSDLAAMVAYADSEGGASLVDYWKEQMGPSSQLDAVSPAKQAARADCPIL
                     LIHGTDDTVVPYNQSERMESALKSAGKPVEFITYKGQDHWETVGSSRVEMMKSALAFL
                     QKHNPA"
     gene            853..>1917
                     /locus_tag="VF402_00725"
     CDS             853..>1917
                     /locus_tag="VF402_00725"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX7798839.1"
                     /translation="MKTRQMTCRPTMRKTAALAAFVGLSALCAAAPSLAATATQGEFG
                     KLADGTAIESVTLKNSHGLSARIITYGAMWQSMMVPDRNGKLADVILGYDTIGEYVAR
                     NQYSGMTVGRYANRIAKGQFTLDGKSYQLAINNPPNALHGGPTGFEVRVWKIVNVDKG
                     GAGKPASVTLQYVSADGEEGYPGTMTTDVTYSLSDNDDLTIHYKAVTDKPTVVNLTNH
                     ALFNLEGAASGHSALNATAQVEADTYLPTDKTAIPTGDCVPVKGTAFDFTKPAVINGR
                     AHDGKDPQVLIGTGIDHNFCLRGGETKTPKLAVTLIDHQNGRGLKILTTEPGIQFYSG
                     NFLDGITPGKGGVTTRQGDGV"
BASE COUNT          372 a          679 c          589 g          277 t
ORIGIN      
        1 atcgtcctgc cgcacggcgg gccgcgcgcg cgcgactaca tcgaattcga ctggcaggcg
       61 caatgcctcg ccgccaacgg ctatgcggtg ctgcaaccca attatcgcgg ctccaccggc
      121 tacggtcagg ccttcctcga cgccggcaac ggccagtggg ggcgcaagat gcagaccgac
      181 ctcagcgacg gcgtgcgcta tctggccgcc agggggctgg tcgatccgaa gcgcgtcgcc
      241 atcctgggcg cctcctacgg cggttacgcc gcgctggcgg gcgcgacgct cgatccgggc
      301 gtctataact gcgccgtctc catcgccggg ccttccgatc tggcggcgat ggtcgcctat
      361 gccgacagcg agggcggcgc ctcgctcgtc gactactgga aggagcagat ggggccgtcc
      421 tcccagctcg acgccgtgtc gcccgccaaa caggcggcca gggccgactg cccgatcctg
      481 ctgatccacg gcacggacga caccgtcgtg ccttacaatc agagcgaacg catggaaagc
      541 gcgctcaaat cggcgggcaa gccggtggag ttcatcacct ataaaggtca ggaccactgg
      601 gagacggtcg gctccagccg cgtcgaaatg atgaagtccg ccctcgcctt cctgcaaaaa
      661 cataatcccg cctgatgcgg cttatggacg cggcgcgcag ggaaatttag aaaagtccgc
      721 gcgccctatt gtccgactat tgcgacaggt ctatgagaga cgtgcgcccc gcgcgcgccg
      781 tcgccggcgc gcgcgcggtc ttcgcgtccg aacggcgcca gaaaccacaa aaagacccaa
      841 gggagggact ttatgaagac tcgccaaatg acctgccgcc ccacaatgcg caagacggcg
      901 gcgctggccg ctttcgtcgg cctttcggcc ctgtgcgccg ccgcgccgtc gctggccgcc
      961 accgcgacgc agggcgagtt cggcaagctg gccgacggaa cggcgatcga gtcggtgacg
     1021 ctgaagaaca gccacggcct gtcggcgcgc atcatcacct acggcgccat gtggcagtcg
     1081 atgatggtcc ccgaccgcaa cggcaagctg gccgacgtga ttctcggcta tgacaccatc
     1141 ggcgaatacg tcgcccgcaa ccagtattcc ggcatgacgg tcggccgcta cgccaaccgc
     1201 atcgccaagg gccagttcac gctggacggc aagtcctatc aactggccat caacaatccg
     1261 cccaacgcgc tgcacggcgg cccgaccggc ttcgaggtgc gcgtctggaa gatcgtcaat
     1321 gtcgacaagg gcggcgcggg caagcccgcc agcgtgacgc tgcaatacgt ctccgccgac
     1381 ggcgaggaag gctatccggg gacgatgacc acggacgtga cctattcgct cagcgacaat
     1441 gacgacctga cgatccacta caaggccgtc accgacaagc cgaccgtcgt caacctgacc
     1501 aaccacgccc tgttcaatct cgaaggcgcc gcctccggcc acagcgcgct gaacgccacc
     1561 gcacaggtcg aggccgacac ctacctgccg accgacaaga ccgcgattcc gaccggcgat
     1621 tgcgtgccgg tcaagggcac ggccttcgac ttcaccaagc ccgccgtcat caacggccgc
     1681 gcccacgacg gcaaggatcc gcaggttctg atcggcacgg gcatcgacca taatttctgc
     1741 ctgcgcggcg gcgagaccaa gacgccgaaa ctggccgtca ccctgatcga ccatcagaac
     1801 ggccgcggcc tgaagatcct gaccaccgag ccgggcatcc agttctattc cggcaacttc
     1861 ctcgacggca tcacccccgg caagggcggc gtgaccacgc gtcagggcga cggcgtc
//
LOCUS       DASVLL010000041         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_109685,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000041 DASVLL010000000
VERSION     DASVLL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109685"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF402_00730"
     CDS             <1..290
                     /locus_tag="VF402_00730"
                     /inference="COORDINATES: protein motif:HMM:NF037461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX7798840.1"
                     /translation="EANGAMRLAVTLRVNAAPSGAVKMGVGAANLPITAPLQAAGAGY
                     AQIAVPLSCFKGQDLSKTPTVLRLETAGTLDVSISDVRLTEAQGDGACPAD"
     gene            complement(287..553)
                     /locus_tag="VF402_00735"
     CDS             complement(287..553)
                     /locus_tag="VF402_00735"
                     /inference="COORDINATES: protein motif:HMM:NF022786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HEX7798841.1"
                     /translation="MSDAQDKFVQMASAHIDLANQHGKTAGAELVAIAINHAAARYAA
                     FMVTQTEPERLARDREAIVTRLVDEYRDMLNQHYDEYVKAADKA"
     gene            695..1132
                     /locus_tag="VF402_00740"
     CDS             695..1132
                     /locus_tag="VF402_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798842.1"
                     /translation="MTPAQQHAASLAWQHAHPLMIVAISTIASLVIIALIVLIRWLVS
                     ARAWKYHPGGAGGFLGDECVRWAAILIPYLALSIGFKIFVYDLHPEYNRPQVWIGFVV
                     AAIAFRIGLRRLPFIRAMARHIDAAKAQAKAQARAQAGAAKRS"
     gene            1135..1995
                     /locus_tag="VF402_00745"
     CDS             1135..1995
                     /locus_tag="VF402_00745"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX7798843.1"
                     /translation="MVQAALTAFPPDFRHDGRVAIDLRVPSAPRPAPPPTEIHDARAR
                     QGDQDPAAFFARHGFTLLRHETAITDWDAPPPSRCPPGYLDELETLIRTRLLPGRRIE
                     IPPVASLVRRGRDTTTPAYGTGVHQDCGLGVDDYQQLVGAFAGDQAAAWWRAGYDRAE
                     VESYLLIDFWRTTNMDEPLRHMPLALCDPNSVDPADIVPTEIHGIAPDGRAPHQMALR
                     RNPEQRWYSYPGMTGDEVLAFKLFEARKSDPEPRLRACFHSAFEDPAAPADAQPRQSC
                     ECRVGVFVMR"
     gene            2072..3055
                     /locus_tag="VF402_00750"
     CDS             2072..3055
                     /locus_tag="VF402_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7798844.1"
                     /translation="MKTRKLGQNLEVSALGLGCMGMSWAYGPSDRDACLKLLREAVDL
                     GVTFFDTAEIYGPFTNEELVGEALRPVRDKVTIATKFGFVTNQKPGSLTHVDSRPEHI
                     REVTDASLKRLGVETIDLLYQHRVDPNVPIEDVAGTVADLIEAGKVRHFGLSEASAAT
                     IRRAHEVQPVAAVQSEYSLWTRDPETDILPTLEELGIGFVPYSPLGRGFLTGALQDAP
                     LDPSDYRNTSPRFVGEAAKTNARAVDALKDLAAAKGVTPAQLALAWLLAQKPWIVPIP
                     GTRRPERLKENIASADIELTRADLDAIEDAIAKTGFAGARYTEGGMAMLNR"
BASE COUNT          570 a         1146 c         1045 g          549 t
ORIGIN      
        1 gcgaggccaa cggcgcgatg cggctggccg tcaccctgcg cgtcaacgcc gccccgtccg
       61 gcgcggtcaa aatgggcgtc ggcgcggcca acctgcccat aacagcgccg ttgcaggctg
      121 cgggcgcagg ctacgcgcag atcgccgtgc cgctgtcctg cttcaagggg caggacctgt
      181 cgaaaacgcc caccgtcctg cggctggaga ccgccgggac gctggacgtg tcgatctcgg
      241 acgtgcgcct gaccgaggcg cagggcgacg gcgcctgccc ggcggattaa gccttgtccg
      301 ccgccttcac atattcgtcg tagtgctggt tcagcatgtc gcgatattca tcgaccagtc
      361 gcgtgacgat ggcctcgcgg tcgcgggcca gccgctccgg ctcggtctgc gtcaccatga
      421 aggcggcgta gcgcgcggcg gcgtggttga tggcgatggc gaccagctcc gcccccgccg
      481 tcttgccgtg ctggttggcc agatcgatat gggcggaagc catctgtacg aacttgtcct
      541 gagcgtcgct cacgaagcgc ctccttgtgc ggatgtgatt gttgctgagc agatatgcgc
      601 aggaacaccc tcaagtcaat tcaggatacg cgccgcatcc aaacggcgcg gcggcggaca
      661 ttaaagcggc gaagacacca tgaaaggcct ccgcatgacc cccgcccagc aacacgccgc
      721 ctcactggcc tggcagcacg cccatccgtt gatgatcgtg gcgatctcga ccatcgcctc
      781 gctcgtgatc atcgcgctca tcgtgctgat ccgctggctg gtcagcgcgc gggcgtggaa
      841 atatcatccc ggcggcgcag gcggcttcct cggcgacgaa tgtgtgcgct gggcggcgat
      901 cctgattccg tatctggcgc tcagcatcgg ctttaaaatc ttcgtctatg acctgcatcc
      961 ggagtataac aggccgcagg tgtggatcgg cttcgtcgtc gccgccatcg ccttccgcat
     1021 agggttgcgc cgcctgccct tcatccgggc gatggcccgc catatcgacg cggccaaggc
     1081 gcaggccaaa gcgcaggcca gagcgcaggc cggggccgcg aaacggagct gatcatggtt
     1141 caggccgccc tcaccgcctt tccgccggat ttccgccatg acgggcgcgt cgccatcgac
     1201 ctgcgcgtcc cgtccgcgcc gcgtccggcg ccgccgccga ccgagatcca cgacgcccgc
     1261 gcgcggcaag gcgaccaaga cccggcggcc tttttcgcgc gccacggctt tacgctgctg
     1321 cggcacgaga cggccattac cgactgggac gcgccgccgc ccagccgttg cccgcccggc
     1381 tatctggacg agctggagac gctgatccgc acgcgcctgc tgcccggacg ccggatcgag
     1441 attccgcccg tcgcctcgct ggtccggcgc gggcgcgaca cgacgacgcc cgcctacggg
     1501 accggcgtgc atcaggactg cggcctcggc gtcgacgact atcagcaact ggtcggcgcc
     1561 ttcgcgggcg atcaggccgc cgcgtggtgg cgcgccggct acgaccgcgc cgaggtcgaa
     1621 tcctatctgc tgatcgactt ctggcgcacg accaacatgg acgaaccgct gcgccacatg
     1681 ccactggcgc tgtgcgatcc gaactcggtc gatccggcgg acatcgtgcc gaccgagatt
     1741 cacggcatcg cccccgacgg cagggcgccg caccagatgg ccctgcgccg caacccggag
     1801 cagcgctggt attcttatcc cggcatgacc ggcgacgagg ttctggcctt caagctgttc
     1861 gaggcgagga agtccgaccc cgaaccgcgc ctgcgcgcct gcttccactc ggcgttcgaa
     1921 gaccccgccg cgcccgccga cgcccagccg cgccaaagct gcgaatgccg cgtcggcgtg
     1981 ttcgtcatgc ggtaacattt gtcgtgaagt cctttcgccg cccgcctgcg cgcctcagat
     2041 aaggcgtcgt tcggcgataa ggagccttgt catgaaaaca cgcaagctgg gtcaaaatct
     2101 cgaagtctcg gcgctcggcc tcggttgcat gggcatgagc tgggcctacg ggccgtccga
     2161 ccgcgacgcc tgcctcaaac tgctgcgcga ggcggtcgat ctcggcgtca ccttcttcga
     2221 caccgccgaa atctatggcc cgttcaccaa tgaggagctg gtcggcgagg cgttgcgccc
     2281 cgtgcgcgac aaggtgacga tcgccaccaa gttcggcttc gtcacgaacc agaagccggg
     2341 cagcctgacc catgtcgatt cccgccctga acatatccgc gaggtgaccg acgcctcctt
     2401 aaaacggctc ggcgtcgaga ccatcgacct tctgtaccag caccgcgtcg atccgaacgt
     2461 gccgatcgag gacgtggccg gaaccgtggc cgacctgatc gaggcgggca aggtgcgcca
     2521 tttcggcctc tccgaagcct ccgccgccac catccgccgc gcccacgaag tgcagccggt
     2581 cgcggcggtg cagagcgaat acagcctgtg gacgcgcgac ccggagaccg acatcctgcc
     2641 gactctggag gagctgggca tcggtttcgt gccctattcg ccattggggc gcggcttcct
     2701 gaccggcgcg ctgcaagacg cgccgctcga tccgtcggat tatcgcaaca ccagcccgcg
     2761 cttcgtcggc gaggcggcga aaaccaacgc ccgcgcggtc gacgcgctga aggatctggc
     2821 cgccgccaaa ggcgtgaccc ccgcgcaact ggcgctggcc tggctgctgg cgcaaaagcc
     2881 atggatcgtc ccgattcccg gcacgcgccg tccggagcgg ctgaaggaga atatcgccag
     2941 cgccgacatc gagctgacgc gcgccgatct ggacgctatc gaggacgcga tcgcgaagac
     3001 cggtttcgcg ggcgcgcgct acaccgaggg cggcatggcc atgctcaacc ggtaaacatg
     3061 aaaaaggccc cggtcgtccg ccggggcctc atcgtttcta gagcgctttc cgaaaagtgt
     3121 gacgcacttt tcggattaaa aagcgcgtaa aagcataaaa ttagagcgcc gatctgatgc
     3181 aatcagatcg gaaaacgctc taaattccgg cccgcatccc atgcgcgtag attcgcgcag
     3241 ggattgcaat ccgggaatcg tctcgatcct caaactagtc cggcgagcgt taatgaatgt
     3301 ttaccatttt
//
LOCUS       DASVLL010000042         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_485628,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000042 DASVLL010000000
VERSION     DASVLL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485628"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VF402_00755"
     CDS             complement(<1..532)
                     /locus_tag="VF402_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798845.1"
                     /translation="MKDKNYRFAYDVGTNSLGCAVYELDNKNNPIDLTDTGVRIFSDG
                     RDPKSGESLAVERRGPRAMRRRRDRYLQRRAYLMDLLIKAGLMPQDEAARKALEKLDP
                     YVLRARGLDEALSPHEFGRVLFHLNQRRGFQSNRIADAGKDEDSGKIKTAEKKLMAAL
                     EAQGCRTYGEFLAKRQA"
     gene            complement(973..1737)
                     /locus_tag="VF402_00760"
     CDS             complement(973..1737)
                     /locus_tag="VF402_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7798846.1"
                     /translation="MSEAAEAFVIAQTFSAAPRARFQTDRHYLLYARAGMMILEAGGR
                     RWTLPPARAAIIPADAPIHVTLPRPVEAASILFDPAVFAAPAAALTVIDMSPLALELV
                     RACSVFTDRRRPLDAYARQLFATLAMVVDRLAQRPSPVGMPLARSREVIEAVRIAETR
                     LTRPPEFETVAAMAATSPRSLARKFSCELGLTWRQTVQRLRIIRAIDLLSETSASITT
                     VAFAVGYESLSAFNAGFRGLTGVTPSEYRKAISSAD"
     gene            complement(1751..>2008)
                     /locus_tag="VF402_00765"
     CDS             complement(1751..>2008)
                     /locus_tag="VF402_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX7798847.1"
                     /translation="REAALAELLTDADAVRAMKGNIGFRCYLDPVIPEALCIVHEWQD
                     AEAFAAYTNSRYFSRLNAVLRPMMTSRPISRRMKAALIESV"
BASE COUNT          373 a          628 c          607 g          400 t
ORIGIN      
        1 cggcctgacg tttggccaga aactcaccgt aggtgcggca gccttgcgct tccagggcgg
       61 ccatcagctt cttttcggct gttttgatct tgccgctgtc ctcgtccttg ccggcatcgg
      121 cgatgcggtt ggactggaaa ccgcgccgct gattgagatg gaacaagacg cggccgaact
      181 catgcggaga cagggcctcg tccaatcccc tggctctcag aacatacgga tcgagttttt
      241 cgagcgcctt gcgtgcggcc tcgtcctgcg gcattaggcc tgctttgatc agcaggtcca
      301 tcaggtaggc gcggcgctgt agatagcggt cacgccgccg gcgcatggcg cgcgggccac
      361 gacgctccac agccagcgat tcgccgcttt tcgggtcacg cccatctgaa aagatacgca
      421 cgccggtatc ggtcagatca atgggattgt tcttgttgtc caactcataa acggcgcacc
      481 cgagtgaatt ggtgcccaca tcgtaggcga atctgtaatt tttgtccttc atggccccct
      541 gccatttttg gctgtacatc atccagcttg acacaacttt tgcatcatgt tcaatgagca
      601 atgtcttagc agagacaacg gcggggtccc tccgttaaca agggatttat cccaccaaat
      661 tgagggcagt ccaagggctg ccctttttgc tgcgtcatca agccagacgg acgtagcttc
      721 ggccatcgtc gaaggtttcg atttcgccaa ggcggtccag cgacgccagc acctcttcag
      781 tcgccgacta ccgcaaagac aagcccattc ctcgtcatat tggcctggcg accccccgtt
      841 gcctgataac accaagccgg cgcctgatat gaccggtttt tcctgttttc cctgataaca
      901 gggggaaact cggccatgag gctggatagc tgcgccacca cgtcttcgcc tgatgtttat
      961 tatgcggtca gctcaatccg cggatgaaat cgctttgcgg tattccgatg gcgtgacgcc
     1021 cgtaaggccg cgaaatcccg cattgaaggc cgagagcgac tcgtagccga cggcgaaggc
     1081 cacggtcgtg atgctggccg acgtttccga cagcaggtcg atggcgcgga ttattctcag
     1141 tctttgcacc gtttgccgcc aggtcagccc cagttcgcag gaaaatttgc gggcgagcga
     1201 cctcggactc gtggccgcca tcgccgccac ggtctcgaat tcgggcggcc gcgtcaaccg
     1261 ggtttcggcg atgcgcacgg cttcgatgac ctcgcgcgac cgcgccaagg gcatgccgac
     1321 cggcgacggg cgctgcgcca ggcggtcgac gaccatcgcc aatgtcgcaa acaactgccg
     1381 ggcgtaggcg tcgagcggcc gcctgcgatc ggtgaaaaca ctgcaggccc gcaccagctc
     1441 cagcgcgagc gggctcatgt cgatgaccgt caacgccgcc gccggcgccg cgaacacggc
     1501 gggatcgaac agtatcgagg cggcttcgac cggccgcggc aaggtgacgt ggatgggcgc
     1561 gtcggcggga atgatcgcgg cgcgcgccgg aggcagcgtc cagcgccgcc cgcccgcttc
     1621 cagaatcatc atgcccgcgc gcgcatacag aagatagtga cgatcggtct ggaaccgggc
     1681 gcgcggcgcg gcggaaaaag tctgcgcgat tacaaacgct tccgccgctt cgctcatatc
     1741 cgcatgccct ctaaaccgat tctatcaacg ccgctttcat tctgcggctg atcggcctgg
     1801 acgtcatcat cggacgcaga acggcgttca accggctgaa gtatcgggag ttcgtgtagg
     1861 cggcgaaggc ttcggcgtcc tgccattcat gcacgatgca cagcgcttcg ggtatgacgg
     1921 gatcgaggta gcagcggaag ccgatattgc ctttcatggc cctgaccgcg tcggcgtcgg
     1981 tgagaagttc cgcgagcgcc gcttcccg
//
LOCUS       DASVLL010000043         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_55668, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000043 DASVLL010000000
VERSION     DASVLL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55668"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..986)
                     /locus_tag="VF402_00770"
     CDS             complement(213..986)
                     /locus_tag="VF402_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7798848.1"
                     /translation="MSEPTPEAKPALLREIFTQANIAVGLAVFAVVLAAAPYVVPPVR
                     TWMVREAMLSRPDMLQDASDKLRAQMQAKADQALADGVRTHHDSLFNDASDPVLGDPK
                     APIKIVEFLDYNCAFCRAATPVLKDYLAQNPDVAIIVKEYPVINQDSPLLAAYALAAA
                     QQGKYAQMHYALMTAKAENVAALDDLAGKNGLDLQKAHDFIKSDAARDHIGKVMNLGA
                     DLDITGTPTFVVGDQAINGAKMDDLKAAVAAQRKALKKG"
     gene            complement(1003..2427)
                     /locus_tag="VF402_00775"
     CDS             complement(1003..2427)
                     /locus_tag="VF402_00775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7798849.1"
                     /translation="MQDFRAYLMSALRATRRIALPALGACLILAPAATAQDIDPDSQS
                     VIRDTEIETFLKTQTRPILAAAGLNPDDVHYLLIASSDLNAFSTFRLVIGLNTGLIME
                     ADNPNQLFGVIAHETGHLSGGHMMRTDEIERAGRAPMAISLGLGIVAALAGAPDAGLG
                     IAASSPTFGALNQLHYMQTEESAADVAGVKAMEKAGMSGKGLVDFFYKFRNVETFSHA
                     ERYEFFRTHPLSRERIQALTDFAKKQPHWNTPDDPAVVAQFAIVKAKLAGFLDDPLKT
                     FQLYPESDHSFAARYARVIATYKQGNWDKALSDLDGLIQENPNNPYLWELKGQIYFET
                     GRPQLAKPAHEKSVELMPTAALLQLNLGQTLIAIGDKDDLNEAVQHLQDSIKYEKDDG
                     FAWEQLAQAYDALKQPGLARLATAEAQFYEGDYNSARTSAVWSQKYFDPQSPEFRRAR
                     DIVMATSSELGVPPVNGETKRRRP"
BASE COUNT          394 a          722 c          842 g          476 t
ORIGIN      
        1 ctcgacctga tgggccgcgg ccagaagccc cccgccgaaa aagcggcgcg ggcggtgctg
       61 gagtttttga agatttaagg ttccgccgcg aaccgtccgg ccttgccgaa gtcttttttg
      121 acacggacga tggccttcgg ccatccccac gaaccgccgc tgagtgacgt acacggaagg
      181 gtgacgccac ctgtttccgc cgctgaaaaa tcctatcctt ttttcagcgc cttgcgctgg
      241 gcggcgacgg cggctttgag gtcgtccatc ttggcgccgt tgatcgcctg atcgccgacg
      301 acgaaggtcg gggtgccggt gatgtcgagg tcggcgccga gattcatcac cttgccgata
      361 tggtcgcgcg cggcgtccga tttgatgaag tcgtgcgcct tttgcaggtc gaggccgttt
      421 ttaccggcca gatcgtcgag cgcggcgacg ttttcggcct tggcggtcat cagcgcgtag
      481 tgcatctgcg catacttgcc ctgctgcgcg gcggcgaggg cgtaggcggc cagcaggggc
      541 gaatcctgat tgatgaccgg atattccttg acgatgatgg ccacgtccgg gttctgcgcc
      601 agatagtcct tcagcaccgg cgtcgccgcg cggcagaagg cgcagttgta gtcgaggaac
      661 tccacgatct tgatcggcgc cttcggatcg cccagcaccg gatcgctggc gtcgttgaac
      721 agcgaatcgt gatgggttct gacgccgtcg gccagcgcct gatcggcctt ggcctgcatc
      781 tgcgcgcgca gcttgtcgga ggcgtcctgc aacatgtcgg ggcggctcag catcgcttcg
      841 cgcaccatcc atgtccgcac cggcgggacg acatagggcg cggcggccag cacgacggcg
      901 aaaaccgcga ggccgacggc gatattggcc tgggtgaaaa tttcgcgcag cagggcgggt
      961 ttggcttcgg gcgtgggttc ggacaaagag actctcgtga cgttaagggc ggcggcgctt
     1021 cgtctcgccg ttgacgggcg ggacgccaag ctccgatgag gtggccatga cgatgtcgcg
     1081 cgcgcggcgg aattccggcg attgcggatc gaaatatttt tgcgaccaga ccgccgaggt
     1141 gcgggcggag ttgtagtcgc cttcatagaa ctgcgcctcg gcggtggcca gacgcgccag
     1201 acccggctgc ttcagcgcgt cataggcctg cgccagttgc tcccacgcga agccgtcgtc
     1261 cttttcgtat ttgatcgagt cctgcaaatg ctgcacggct tcgttcaggt cgtccttgtc
     1321 gccgatggcg atcagggtct ggccgagatt gagttgcagc agcgccgccg tcggcatcag
     1381 ctcgaccgac ttttcgtgcg cgggcttggc caattgcgga cggccggtct cgaaatagat
     1441 ctggcccttc aattcccaca gatagggatt gttcggattt tcctgaatca ggccgtcgag
     1501 atcgctcagc gccttgtccc agttgccctg cttataggtg gcgatgacgc gggcgtagcg
     1561 ggcggcgaag ctgtggtcgc tttcgggata gagctggaag gtcttgagcg ggtcgtcgag
     1621 gaacccggcc agcttggcct tgacgatggc gaactgcgcg accaccgccg gatcgtcggg
     1681 cgtgttccag tgcggctgct tcttcgcgaa gtcggtcagg gcctggatgc gctcgcgcga
     1741 cagcgggtgc gtgcggaaga actcatagcg ctcggcgtgg ctgaaggttt cgacattacg
     1801 gaatttatag aagaaatcga ccagcccctt gccggacatg ccggcctttt ccatcgcctt
     1861 gacgcccgcg acgtcggcgg cggattcctc ggtctgcatg tagtgcaact gattgagcgc
     1921 gccgaaggtc ggcgacgagg cggcgatgcc gaggcccgcg tccggcgcgc cggccaaagc
     1981 cgcgacgatg ccgaggccga gtgaaatcgc catcggcgcg cggcccgcgc gctcgatctc
     2041 gtcggtgcgc atcatgtggc cgccgctcag gtggccggtt tcgtgcgcga tgacgccgaa
     2101 caactggttc ggattgtcgg cctccatgat caggccggtg ttcaggccga tgacgaggcg
     2161 gaaggtcgaa aaagcgttca ggtcgctgga ggcgatcagc agataatgca cgtcgtcggg
     2221 gttcagcccg gcggcggcca ggatgggccg cgtctgcgtc ttgaggaagg tttcgatctc
     2281 ggtgtcgcgg atgacgcttt gcgaatccgg gtcgatgtcc tgcgccgtgg cggcgggcgc
     2341 caggatcagg caggcgccca gcgccggaag cgcgatccgg cgcgtcgcgc gcagcgcgga
     2401 catcagatag gcgcgaaaat cctgcatcgc aaag
//
LOCUS       DASVLL010000044         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_324623,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000044 DASVLL010000000
VERSION     DASVLL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324623"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /gene="hisE"
                     /locus_tag="VF402_00780"
     CDS             <1..273
                     /gene="hisE"
                     /locus_tag="VF402_00780"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX7798850.1"
                     /translation="GKLAEVVAARAKAPPADSYTARLMQKGVAKIAQKVGEEGLETAL
                     AGRCGDDEELYNEAADLLYHLSVLLMARGASLENVLAVLRERHAGR"
     gene            329..832
                     /locus_tag="VF402_00785"
     CDS             329..832
                     /locus_tag="VF402_00785"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX7798851.1"
                     /translation="MIDLSWDTHGRIDRDAYRKARWRHFAFCLIPAVLFVLGGIGATT
                     TLIWLSVLAWIAGFVLLSYYRYRFMQVMVRRLHDRNLSGWVMLLSPFVLVLALTVGGV
                     WLFTTVANAASPSWLNDTTTKFGIWIVLLPAIAINVFLRYQLTRPGTPGPNKYGLPPG
                     VGQAQVF"
     gene            complement(829..1818)
                     /locus_tag="VF402_00790"
     CDS             complement(829..1818)
                     /locus_tag="VF402_00790"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX7798852.1"
                     /translation="MTGFAPVRRLTLRREAIADAVRDLDAPVVFTGAVADWPLARQPS
                     ADALIDYLKRFDAGRSAQVFRQAAGGDGRFFYGEGADGFNFQRADVPVAAALERLRAL
                     AKGGDERVYIQSAPLDLYFPGLAAANPLPGIAAGPRAWIGNRAVTRTHFDLYDNLVCM
                     IAGTKRFILFPPEQTKNLYIGPLEHTVSGVPASMADIDAPDFARFPRLREALAAGLIA
                     ELTPGDVLFIPYMWWHQVMSDDAFNMQVNYWWNPAADLPQPMQALAHALLAVRDLPPA
                     QNRAWKAMFDHFVFHQSDPVADHLPEAMRGILGDLTPEQRAAMLKALGQGLQD"
     gene            1900..2304
                     /locus_tag="VF402_00795"
     CDS             1900..2304
                     /locus_tag="VF402_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798853.1"
                     /translation="MLSLYLSPRGRIDRTGFIAGLGGVLLFALLLDLALAALPGVNEA
                     LILSGVWALLAWPAVCLLIKRFRDTGSGPGGLWLFAPQIFVALAALAMSGAWPDALLT
                     GFTWLGVAANLLCWGALVWLAAAPSRRHKSAL"
     gene            2383..2568
                     /locus_tag="VF402_00800"
     CDS             2383..2568
                     /locus_tag="VF402_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798854.1"
                     /translation="MSWLHNLLSSLIAFLLAVVLAHFGAHGASGDASGGALGDGRNAP
                     APHSDASAAPARDSASR"
BASE COUNT          415 a          922 c          874 g          450 t
ORIGIN      
        1 ggcaagctgg ccgaagtggt cgccgcgcgc gccaaggcgc cgcccgccga ttcctacacc
       61 gcccgcctga tgcagaaggg cgtcgccaag atcgcccaaa aggtcggcga ggaaggtctg
      121 gagacggcgc tggccggacg ctgcggcgac gatgaggagt tgtacaacga ggcggcggac
      181 ctgctctatc acctcagcgt gctgctgatg gcgcgcggcg cgagccttga aaacgtgctg
      241 gccgtcctgc gcgaacgcca cgcggggcgg tagtttacaa cccgcccgca tcgtgctttc
      301 cttctccccg acaaaagggg ggacaatcat gatcgatctg agttgggaca cgcacggacg
      361 catcgacagg gacgcgtacc gcaaggcgcg ctggcggcac ttcgcatttt gcctgatccc
      421 cgccgtcctg ttcgttctgg gcgggatcgg cgcgacgacg acgctgatct ggctatccgt
      481 tctggcgtgg atcgcgggct tcgtcctgct ctcttattac cgctaccggt tcatgcaggt
      541 catggtccgg cggctgcacg accgcaacct gtccggctgg gtcatgctgc tcagcccgtt
      601 cgtgttggtt ctggccctga cggtgggcgg cgtctggctg ttcacgacgg tcgccaacgc
      661 ggcgtcgccg tcatggctga acgacacaac gacgaagttc ggcatatgga tcgtgctgtt
      721 gcctgcgatc gccatcaacg tctttctgcg ctatcagttg acgcggcccg gcacgcccgg
      781 cccgaacaaa tacggcttgc cgccgggcgt cggacaggcg caggttttct aatcctgcaa
      841 cccctgcccc agcgccttga gcatcgccgc gcgctgctcc ggcgtgaggt cgcccaatat
      901 gccgcgcatc gcttcgggca gatggtcggc gaccggatcg gactggtgaa agacgaagtg
      961 gtcgaacatc gccttccacg cccggttctg cgcggggggt aggtcgcgca cggccagcag
     1021 ggcgtgcgcc agcgcctgca tcggctgcgg caagtccgcc gccgggttcc accagtaatt
     1081 gacctgcatg ttgaaggcgt cgtcggacat gacctgatgc caccacatat aggggatgaa
     1141 cagaacatcg cccggcgtca gctccgcgat cagccccgcc gccaacgcct cgcgcaggcg
     1201 cggaaagcgc gcgaaatccg gcgcgtcgat atccgccatc gaagccggaa cgcccgacac
     1261 cgtatgctcc agcgggccga tatagaggtt cttcgtctgc tccggcggga acaggataaa
     1321 ccgcttcgtc cccgcgatca tgcagaccag attgtcgtac aggtcgaaat gcgtccgggt
     1381 gacggcgcgg ttgccgatcc aggcgcgcgg ccccgccgcg atccccggca gcggattggc
     1441 cgccgccagc cccggaaaat agaggtccag cggcgccgac tggatataga cgcgctcgtc
     1501 cccgcccttc gccagcgcgc gcagccgctc cagcgccgcc gccaccggca cgtcggcgcg
     1561 ctggaagttg aagccgtccg cgccctcgcc gtagaagaaa cgcccgtcgc cgcccgcggc
     1621 ctgccggaac acctgcgccg accggcccgc gtcaaagcgt ttcagataat cgatcagcgc
     1681 gtccgccgac ggctggcgcg ccagcggcca gtccgccacg gcgcccgtaa agaccaccgg
     1741 cgcgtcgaga tcgcgcacgg cgtccgcgat cgcttcacgg cgcaaggtca ggcgcctcac
     1801 gggcgcaaat ccggtcatgt cctttcctag cccaaaaccg gaccgccttg caatcggcgc
     1861 cgcatttaca gccgcgcgcc cgcccggtat catcgcgcca tgctgagcct ttacctgtct
     1921 ccgcgcggac gcatcgaccg caccggcttc atcgccggtc tgggcggcgt cctgctgttc
     1981 gccctgctgc tcgatctggc gctggccgcc ctgccggggg tcaatgaggc gctgatcctg
     2041 tcgggcgtgt gggcattgct ggcgtggccg gcggtctgcc tgctgatcaa gcgtttccgc
     2101 gacacgggtt ccgggccggg cggcctgtgg ctgttcgcgc cgcaaatctt cgtcgccctc
     2161 gccgcgctgg cgatgtcggg cgcatggccg gacgcgctgc tgaccggctt cacatggctc
     2221 ggcgtcgcgg ccaatctcct gtgctggggc gcgctggtct ggctggccgc cgcgccttcg
     2281 cgccgacata aatccgcact ctaaacgcga gcggcgatgg acagcgaacg cggttcagcg
     2341 catgatgcgc cgggttaagg agtcaggctc aggggaacgg gcatgtcgtg gctgcacaat
     2401 ctgctcagtt cgctgatcgc cttcctgctg gccgtggtgc tggcgcactt cggcgcgcac
     2461 ggcgcctccg gcgacgcttc gggcggcgcc cttggggacg gcagaaacgc cccggcgccg
     2521 catagcgacg cctccgccgc tccggcgcgc gacagcgcct cgcgctgaaa cttttgacaa
     2581 aaagctttgc catcggcggg cctaaaggct agctttcgcc ttgagaggga acctgctgga
     2641 aaaacggccg tttcccgccg g
//
LOCUS       DASVLL010000045         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_217181,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000045 DASVLL010000000
VERSION     DASVLL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217181"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="gltX"
                     /locus_tag="VF402_00805"
     CDS             <1..1094
                     /gene="gltX"
                     /locus_tag="VF402_00805"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7798855.1"
                     /translation="CLMTPEETEAAREQARADGHALRSPWRDRTPSADDLRKPHVVRF
                     KGPLNAPLLIRDAVQGEVAFNTKDMDDLILLRSDGAPTYNLAVVVDDHDMGVTHVIRG
                     DDHLNNAARQTLIYQALDWPTPTFAHIPLIHGPDGAKLSKRHGAQAVGDYEDLGYLPE
                     AMRNYLARLGWSHGDDEIFSDDQAIQWFDIADVNKAPARLDFAKLGSVNAHWIRVCDP
                     ERLTDLVAKRLNLTDAAQRAVLARTLPLVRERAQTLAQLVDLAQFALAKGPFALDEKT
                     KSLLTGDTVARLRRLHENVLSWDAWDAGAIDAALKNFVENEGIGFGKIGPVLRGLLSG
                     GHPAPDISRVFASLGREESLSRLQQGLFI"
     gene            1183..>2326
                     /gene="gltA"
                     /locus_tag="VF402_00810"
     CDS             1183..>2326
                     /gene="gltA"
                     /locus_tag="VF402_00810"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX7798856.1"
                     /translation="MTQTTDANLKLGDKAVDLPVLKGTLGPDVVDIRKLYGATDAFTY
                     DPGFTSTASCESKITYIDGDAGVLLHRGYPIGELAAKSNFLEVCYLLLHGELPNVAEF
                     EDFETTITRHTMLHEQFDKFFSGFRRDAHPMAVMTGAVGALSAFYHDSLDIHDLKQRE
                     ISAHRLIAKMPTIAARAFKYNLGQPFVHPRNNLSYAENFLRMCFAVPAEDYEVNPVLA
                     KAMDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPSHGGANEEALNM
                     LKEIGTPERIPEFIAGVKDRKYKLMGFGHRVYKNYDPRAKVMKESADEVLAAVGDPND
                     PLFQVAKELEKIALNDEYFIERKLFPNVDFYSGITLSAMGFPTTMFT"
BASE COUNT          446 a          773 c          699 g          408 t
ORIGIN      
        1 cctgcctcat gacgccggag gaaaccgagg ccgcgcgtga gcaggcgcgc gccgatggtc
       61 acgcgctgcg ctcgccgtgg cgcgaccgga cgccctccgc cgacgacctg cgaaagccgc
      121 acgtcgtgcg cttcaagggg ccgctcaatg caccgctgct cattcgcgac gcggtgcagg
      181 gcgaggtcgc tttcaatacg aaagacatgg acgatctgat cctgctgcgc tcggacggcg
      241 cgccgaccta caatctggcc gtggtggtgg acgatcacga catgggcgtc acccacgtca
      301 ttcgcggcga cgaccacctc aacaacgccg cgcgccagac cctgatctat caggcgctgg
      361 actggccgac gccgaccttc gcgcacatcc cgctgatcca cgggcccgac ggcgccaagc
      421 tgagcaagcg ccacggcgcg caggcggtcg gcgattacga ggatctcggc tacttgcccg
      481 aagcgatgcg caactatctg gcccggctcg gctggtcgca cggcgacgac gagattttca
      541 gcgacgatca ggcgattcaa tggttcgaca tcgccgacgt caacaaggcg cccgcccgcc
      601 tcgacttcgc caagctgggc agcgtcaacg cccactggat ccgcgtctgc gatccggaac
      661 ggctgaccga tctggtcgcc aaacgtctga atctcaccga cgcggcgcag cgcgccgtgc
      721 tggcgcgcac tctgccgctg gtgcgcgaac gcgcccagac cctggcgcaa ctggtcgatc
      781 tggcgcagtt cgctttggcg aaaggcccgt tcgcgctaga tgagaaaacg aaatccctgc
      841 tgaccgggga cacggtggcg cgcttgcggc gtcttcacga aaatgtgtta agctgggacg
      901 cctgggacgc cggcgccatc gacgcggctt tgaaaaattt cgttgaaaat gaaggtattg
      961 gcttcggaaa gatcggaccg gtgctgcgcg gcctgttgtc gggcggccat ccggcgccgg
     1021 atatttcccg cgttttcgcc agcctcggac gcgaagaaag cctgtcgcgg ctgcaacaag
     1081 gtcttttcat ttagacgcgg tgtttatata gaggcgcgac gctgatttaa gttgcgccgc
     1141 acaaacccga aagggtttca ctaattttct gaggcaagcg taatgaccca gactactgac
     1201 gccaatttga aactgggcga caaggccgtt gatttgcccg tgctgaaagg cacgctcggc
     1261 ccggacgtcg tcgatatccg caaactttac ggcgcgaccg acgcctttac ctacgatccc
     1321 ggctttacct ccaccgcctc ttgcgaaagc aagatcacct atatcgacgg cgacgccggc
     1381 gtgcttctgc accgcggcta tccgatcggc gagctggcgg ccaagtcgaa cttcctcgaa
     1441 gtctgctatc tgctgctgca cggcgaactg ccgaacgtcg cggagttcga ggacttcgag
     1501 acgaccatca cgcgccacac catgctgcat gagcagttcg acaagttctt ctccggtttc
     1561 cgccgcgacg cccacccgat ggccgtcatg accggcgcgg tcggcgccct gtcggccttc
     1621 tatcacgaca gcctcgacat ccacgacctg aagcagcgcg aaatctcggc gcaccgcctg
     1681 atcgccaaga tgccgaccat cgcggcgcgc gccttcaagt acaatctggg ccagccgttc
     1741 gtgcatccgc gcaacaacct gtcctacgcc gagaacttcc tgcgcatgtg cttcgcggtt
     1801 ccggccgagg attacgaggt caatcccgtg ctggccaagg cgatggaccg catcttcatc
     1861 ctgcacgccg accacgagca gaacgcctcg acctcgacgg tgcgtctggc cggatcgtcg
     1921 ggcgccaacc cgttcgcctg catcgccgcg ggcatcgcct gcctgtgggg cccgtcgcac
     1981 ggcggcgcca acgaggaagc gctcaacatg ctgaaggaaa tcggcacgcc ggagcgcatc
     2041 ccggaattca tcgcgggcgt caaggaccgc aagtacaagc tgatgggctt cggccaccgc
     2101 gtgtacaaga attacgaccc gcgcgccaag gtgatgaagg aatcggcgga cgaggttctg
     2161 gccgccgtcg gcgatccgaa tgatcccctg ttccaggtcg ccaaggagct ggaaaagatc
     2221 gcgctgaacg acgagtattt catcgagcgc aagctgttcc cgaatgtcga cttctattcc
     2281 ggcatcaccc tgtcggcgat gggcttcccg accaccatgt tcaccg
//
LOCUS       DASVLL010000046         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_378423,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000046 DASVLL010000000
VERSION     DASVLL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378423"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..675)
                     /locus_tag="VF402_00815"
     CDS             complement(127..675)
                     /locus_tag="VF402_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7798857.1"
                     /translation="MPTYNPDDWQAWMRAAQGGDAGAYRRLLAALQPWLTAYFRRRLS
                     GDARGGDSDDLVQMTLLSVHEKRHTYDANAPFLPWIAAVARHKLIDHVRRSGRHVHVE
                     LNEALGLDDGMQPDLAARDVQVLLAQLPKEQARIIALQKLDGLSVDEVAQRTGRSAAS
                     VKVIVHRGLKRLRGLVGGGTDD"
     gene            complement(693..1727)
                     /gene="ruvB"
                     /locus_tag="VF402_00820"
     CDS             complement(693..1727)
                     /gene="ruvB"
                     /locus_tag="VF402_00820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7798858.1"
                     /translation="MARLISGDASDDYEERAQDRALRPQTFDDFVGQAPLKANLKVFV
                     KAAAQRREALDHVLFYGPPGLGKTTLAQIVARELGVGFRTTSGPVLAKAGDLAAILSN
                     LEPNDVLFIDEIHRLNPAVEEILYPAMEDYVLDLIIGDGPAARTVRIDLAPFTLVGAT
                     TRAGLLSTPLRDRFGIPLRLEFYTPDELVRVVMGTARKMGADLSEDGALEIASRSRGT
                     PRVAGRLLRRVRDFAIMDGADAITRAAASKALARLEVDPSGLDHSDRRYLKALLENYG
                     GGPVGLETLAAAIAEARDAVEDMIEPYLLQQGFIQRTPRGRMACARAWLHLGLNPPAQ
                     PPSPGDLFES"
     gene            complement(1731..>2292)
                     /gene="ruvA"
                     /locus_tag="VF402_00825"
     CDS             complement(1731..>2292)
                     /gene="ruvA"
                     /locus_tag="VF402_00825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7798859.1"
                     /translation="EALVECAGVGYIVRCGVRTLADLPDLGREVILHIDSQTREDGTR
                     LYGFLDKDERQTFIALLGVQGVGPKAALAVLDVLTPAQLAQAVAHEDKTSIGRANGVG
                     PKLAQRIAIELKGKILFSGDFIPAATPIAKAAVPSVAGDAVAALIGLGVAEQQARVAV
                     DAALKAAGEGAELPALIRASLKALGR"
BASE COUNT          357 a          818 c          767 g          350 t
ORIGIN      
        1 gggtgataga agacgaggat atagacgacc gccgcgacga cgccgagcag cgcgccgagc
       61 cacaggcggc gatcgcgcag cggatgcgcg ggccgcaggt tcttcgtcag ggcgtcgagc
      121 agcgcgtcag tcatcggtgc cccctccgac cagcccgcgc agccgtttca ggccgcgatg
      181 cacgatcacc ttgaccgagg cggcgctgcg gccggtacgc tgcgcgacct cgtcgacgct
      241 gaggccgtcg agcttttgca gcgcgatgat gcgcgcctgt tccttcggca attgcgccag
      301 cagcacctgc acgtcgcgcg cggcgaggtc gggctgcatg ccgtcgtcga ggcccagcgc
      361 ttcgttcagt tcgacatgca catgccgccc cgaccggcgc acatggtcga tcagcttgtg
      421 ccgcgccacc gccgcgatcc acggcaggaa cggcgcgttc gcgtcgtagg tatggcgttt
      481 ttcatgtaca ctcagaaggg tcatctgcac cagatcgtca ctgtcgccgc cgcgcgcgtc
      541 cccgctcagg cggcggcgaa aataagcggt cagccacggt tgcagcgccg ccagcaaccg
      601 gcggtaggca cctgcgtcgc cgccctgcgc cgcgcgcatc catgcctgcc agtcatcggg
      661 gttatacgtc ggcatgagag gaaggttacg atctaacttt caaacaggtc gcccggggac
      721 ggcggctgcg cgggcgggtt caggcccaga tgcagccagg cgcgcgcgca ggccatgcgg
      781 ccacgagggg tacgctggat aaagccctgt tgcaacaggt aaggttcgat catgtcctcg
      841 accgcgtcgc gcgcctcggc gatggccgcc gccagggtct ccagcccgac cggcccgccg
      901 ccgtaatttt ccagcagcgc cttgagatag cgccggtcgg aatggtcgag gccggacgga
      961 tcgacctcca gccgcgccag cgccttcgac gccgccgcgc gggtaatcgc gtcggcgccg
     1021 tccatgatcg cgaagtcgcg cacccggcgc agcagccgtc cggccacgcg cggcgtgccg
     1081 cgcgagcgcg aggcgatctc cagcgcgccg tcctcggaca ggtcggcgcc catcttgcgc
     1141 gccgtcccca tcaccacgcg caccagctcg tccggcgtat agaattccag ccgcagcggg
     1201 atgccgaacc ggtcgcgcaa cggcgtggac aacaggcccg cgcgcgtcgt cgccccgacc
     1261 agcgtgaacg gcgccagatc gatgcgcacc gtgcgcgccg ccggtccgtc gccgatgatc
     1321 aggtccagca catagtcctc catcgccgga tagaggattt cctcgaccgc cggattgagg
     1381 cggtgaatct catcgatgaa cagcacgtcg ttcggttcga gattggatag gatggcggcc
     1441 agatcgcccg ccttggccag caccggcccg gaggtggtgc gaaagccgac gcccagttcg
     1501 cgcgccacga tctgcgccag cgtcgtcttg ccgaggcccg gcgggccgta gaacagcacg
     1561 tggtccagcg cctcgcgccg ctgcgccgcc gccttgacga acaccttgag attggccttc
     1621 agcggcgcct gaccgacgaa gtcgtcgaag gtctgcggcc gcaaggcgcg gtcctgcgcg
     1681 cgctcttcgt aatcgtcgct ggcgtcgcct gagatcaatc tggccatgac ctatcgcccc
     1741 aacgctttca gcgaggcgcg gatcagggcg ggcagttcgg cgccctcgcc cgccgccttc
     1801 agcgccgcat cgaccgcgac gcgcgcctgt tgctcggcca cgccgaggcc gatcagcgcc
     1861 gccaccgcgt cgcccgcgac ggacggaacg gcggctttgg cgatgggcgt cgccgccggg
     1921 atgaagtcgc cgctgaacag gatcttgccc ttgagttcga tggcgatgcg ctgcgccagc
     1981 ttcggcccga cgccgttggc gcggccgatg ctggtcttgt cctcgtgggc gaccgcctgc
     2041 gccagttgcg ccggagtcag cacgtccagc acggccagag ccgccttcgg gccgaccccc
     2101 tgcacgccca gcagcgcgat gaaggtctgg cgctcgtcct tgtcgagaaa gccgtaaagc
     2161 cgcgtgccgt cctcgcgcgt ctggctgtcg atatgcagga tcacttcgcg gccaaggtcg
     2221 ggtaaatcgg ccaatgtgcg cacgccgcag cgcacgatat agccgacgcc cgcgcattcg
     2281 acgagcgcct cc
//
LOCUS       DASVLL010000047         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_271473,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000047 DASVLL010000000
VERSION     DASVLL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271473"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VF402_00830"
     CDS             <1..1325
                     /locus_tag="VF402_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEX7798860.1"
                     /translation="DRILDAYIARGVHPYLQLGFMPQALSSAPKDEPYQHSWRPGFAY
                     NLIDGAWNAPPKDYKKWEELAYQWTKHNLARYGAAEMSKWYFEVWNEPNGPDYWTGTP
                     QDFYKLHDYAIAGVRRAFPQARVGGPDVAGSGGAFMDGFLKHVTSGQNEATGGTGAPT
                     DVLSFHAKGSPRFIDGHVEMGIANQLRTADAGFAKIAAVPELKDKPIIIGENDPEGCA
                     ACPGPQNAYRNGTLYASYTAAVYPRLWQMAARDGVHLDGALTWAFEFENQPWFPGYRQ
                     LSTNGVDLPVMNVFRLFAKLGPEQIAATSDAQIPLGQIEKTGVRAAPDIGVLATKAPD
                     GQVAVLVWHYHDDDVKGPVAHVSLHLTGLPASLHQAEQWRVDATHGDAFSAWLDMGSP
                     QSPDEKQYARLEAASVMTPQPLPLAPHDGAADLGFDLPRQGVTLLLLK"
     gene            1707..>2636
                     /locus_tag="VF402_00835"
     CDS             1707..>2636
                     /locus_tag="VF402_00835"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX7798861.1"
                     /translation="MRAWLLTALTFAAPPAAAACHVSSAPFGVMRDGRAVAVWRIDDG
                     AISAGVLTLGGILHDLNVPDAKGRRANVVRNLDSLAAYEGRPNVSSLIGRYAGRIGGG
                     GITLDGRFHPLPGNAKGVITHGGKDSFGSRLWSARRLKHGCGIELSYVSPDGDNGFPG
                     ALGVSVRYTLKGRALRLDYTARTTKPTVVNLTDHAYFNLSGHGDVYGQTLRIAATHYL
                     PLDKLHLPTGQIAPVDATAYDWRAPRPLSAGEKLDDTFVLAGRGLKLAATLRDPASGR
                     RLAVRTTQPALVVYTAGGFDGSLRDSAGDPLVAG"
BASE COUNT          453 a          926 c          801 g          456 t
ORIGIN      
        1 ccgaccgcat tctcgacgcc tatatcgcgc gcggcgtcca tccctacctg caactgggct
       61 tcatgccgca ggccctgtcc tcggctccga aggatgaacc gtaccagcat agctggcggc
      121 ccggcttcgc ctacaacctg atcgacggcg cctggaacgc gccgccgaag gattataaaa
      181 aatgggagga gctggcctac caatggacga aacataatct ggcccgctac ggcgcggcgg
      241 aaatgtccaa atggtatttc gaggtttgga acgagccgaa cggtcccgac tactggaccg
      301 gaacgccgca ggacttctac aagctgcacg actatgcgat cgcgggcgtc cgccgcgcct
      361 tcccgcaggc ccgcgtcggc ggaccggacg tcgcaggctc cggcggcgcc ttcatggacg
      421 gtttcctcaa acacgtcaca tccggccaaa acgaagcgac cggcggcacg ggcgcgccga
      481 ccgacgtcct gtccttccac gccaagggca gcccgcgctt tatagacggg catgtcgaaa
      541 tgggcatagc caatcagctc cgcaccgccg acgccggttt cgcgaaaatc gccgccgttc
      601 ccgaactgaa ggacaagccg attatcatcg gcgagaacga ccccgaaggc tgcgccgcct
      661 gtccggggcc gcagaacgcc tatcgcaacg gaaccctcta cgccagctac accgccgccg
      721 tctatccgcg cctgtggcag atggcggcgc gcgacggcgt gcatctggac ggcgccctga
      781 cctgggcgtt cgagttcgag aaccagccgt ggttccccgg ctaccggcaa ttgtccacca
      841 acggcgtcga cctgccggtg atgaacgtgt ttcgcctgtt cgccaaactc ggccccgaac
      901 agatcgccgc gaccagcgac gcgcaaatac cgctcggcca gatcgaaaag accggcgtgc
      961 gcgccgcgcc cgacatcggc gttctggcca ccaaggcgcc ggacggacag gtcgccgtgc
     1021 tggtctggca ctatcacgac gacgatgtga aaggccccgt cgcgcatgtg tcgctgcatc
     1081 tgaccggcct gcccgccagc ctgcatcagg ccgaacaatg gcgcgtcgat gcgacccacg
     1141 gcgacgcctt ttccgcatgg ctcgacatgg gcagtccgca atcgcccgac gaaaagcaat
     1201 acgcccggct ggaagccgcc tccgtcatga cgccgcagcc cctgccgctg gccccgcacg
     1261 acggcgccgc cgatctcggc ttcgacctgc cgcgtcaggg cgtcacgctg ttgctgctga
     1321 aatagcggcc tgcggcggct tgaaaagcgg ccatgtcgca caacggttct ttgtcggcgt
     1381 gacgtataaa tactgatttc cagccttcac ctgaactgac cggcggcgtc cccgccggtc
     1441 ttttttgcga ttgaatatga tcatatgtgc gaaattttat catcttcgat cattttcttg
     1501 ttgcaatcgc tcatcacctc ttgtagaacg atcaggtcga tgactgacgc ggtccgcttt
     1561 cccggcgggc cacggggtcg cttcaacatc ccccctgaag cggtcccttt cactccaagc
     1621 gtttaggcgt tcctaatttc tccctgttgg aatcgctggc cgttttgctt cgatgcaaaa
     1681 cggcctcttt ttttggagcc tgtcccatgc gcgcctggct gttgaccgcc ctgaccttcg
     1741 ccgcgccgcc cgccgccgcg gcctgccatg tgtcgtccgc gccgttcggc gtgatgcggg
     1801 acgggcgcgc ggtcgccgtc tggcgcatcg atgacggcgc gatctcggcg ggggtgctga
     1861 ccctgggggg catactccat gacctgaacg ttcccgacgc caaaggccgc cgcgccaatg
     1921 tcgtgcgcaa tctcgacagc ctcgccgcct atgagggccg cccgaacgtc tccagcctga
     1981 tcgggcgcta tgcgggccgc atcggcggcg gcgggatcac gctggacgga cgcttccatc
     2041 ccctgcccgg caacgcgaag ggcgtcatca cccacggcgg caaggatagt ttcggttcgc
     2101 gcctgtggtc ggcgcgcagg ctgaagcacg gctgcgggat cgagctgtcc tatgtcagcc
     2161 cggacggcga caacggcttt cccggcgcgc tgggggtcag cgtgcgctac accttaaaag
     2221 gcagggcgtt gcggctcgac tacacggcgc ggacgaccaa gcccaccgtc gtcaacctga
     2281 ccgaccacgc ctatttcaat ctgagcggcc acggcgacgt ctatggccag accctgcgca
     2341 tcgcggcgac gcattacctg ccgctcgata agctgcatct gccgaccggc cagatcgcgc
     2401 cggtcgacgc aaccgcctat gactggcgcg cgccaaggcc gctgagcgcg ggcgaaaagc
     2461 tcgacgacac gttcgtgctg gcggggcgcg gcctgaaact cgccgcaacc ttacgcgatc
     2521 cggcgtcggg ccgccgcctc gccgtgcgca cgacccagcc cgctctcgtc gtctatacgg
     2581 ccggcggctt cgacggctcg ctcagggact ccgcgggcga tccgctcgtc gcgggc
//
LOCUS       DASVLL010000048         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_432587,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000048 DASVLL010000000
VERSION     DASVLL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432587"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..939)
                     /locus_tag="VF402_00840"
     CDS             complement(241..939)
                     /locus_tag="VF402_00840"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEX7798862.1"
                     /translation="MKLIKHLANLGYGSRKEVQKLLRMGVFTDAEGQALDDGAAHGDI
                     RYKGRPLDPDFGVVLMLNKPCGYVCSLKDRGQLVYELLPARFAARKPVLSSIGRLDSD
                     TSGLLLFTDDGDYLHRIISPKNHVAKIYEVTCARPVEQRYKEIFASGELVLESEKDAL
                     KPAFLEITGERTARLTLYEGRYHQVRRMFAALGNHVESLHRAQMGDLTLRDLPSGKWR
                     ILTPAERDGLLKSV"
     gene            complement(936..>1695)
                     /locus_tag="VF402_00845"
     CDS             complement(936..>1695)
                     /locus_tag="VF402_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7798863.1"
                     /translation="QREEYRALLARTVDETAAGGVVIACASNLEGARTIEGDLKALCG
                     GVASLSKNKCRVFWAQVDPARLNRGLIAQWAALDAPRTVDSGYLSRPGVFAWDRLDAG
                     SRFLAAHLPAFAGVGADLGAGIGYLSDVALRASPAITRLDLIEAEGRALDLARANVPD
                     ARAAFHWLDATQGLPERYDFIVSNPPFHIDRADRHDLGQGFIRSAAAALKPGGRFWMV
                     ANRRLPYEETLKAAFKRVEAVAANEQYKIVQAVR"
BASE COUNT          309 a          550 c          536 g          300 t
ORIGIN      
        1 cgcgctcgat ctcatattcg gcgtcaccag catgcccatg tttcgttcgc tggcggtgga
       61 ttacccgccg gacgtcgtgg cgcagatgat gaagaaggcc gctcacgcga tcctgaccgc
      121 ctatggattg caggcggatt agagcgtttc cccaaaaagt ggttccactt tttggataag
      181 gaagagcgac aaaacaaaga cttagagcgc cgatctgatt ctatcagatc gaaaagcgct
      241 ctaaacgctt ttcagcaggc cgtcgcgctc ggcgggcgtt aaaatccgcc acttgccgga
      301 gggcaagtct ctgagggtca gatcgcccat ctgcgcgcgg tgcaggcttt cgacatgatt
      361 gcctaaagca gcgaacatgc gccgcacctg atgatagcgg ccttcataca gcgtcaggcg
      421 cgcggttctt tcgccggtga tctccagaaa ggccggtttc agcgcgtcct tttcgctttc
      481 cagcaccagc tcgccggacg cgaaaatctc cttgtaacgc tgttctaccg gacgcgcgca
      541 agtcacttcg tagatcttgg cgacatggtt cttcggcgag atgatgcggt gcagatagtc
      601 gccgtcgtcg gtgaacagca acaggcccga cgtatcggaa tcgagccgcc cgatgctgga
      661 caggaccggc ttcctcgcgg cgaagcgcgc gggcagaagc tcatagacaa gctggccgcg
      721 atccttgagc gaacagacat agccgcacgg cttgttcagc atcagcacga cgccgaaatc
      781 gggatcgagc gggcggccct tatagcggat gtcgccatga gcggcgccgt catccagcgc
      841 ctgcccttcg gcgtcggtga aaacgcccat gcgcagcagt ttctgcacct ccttgcggct
      901 gccgtagccg agattggcga gatgtttgat cagcttcatc gcaccgcctg cacaatcttg
      961 tactgttcgt tggcggcgac cgcctcgacg cgcttgaagg cggctttcag cgtctcctca
     1021 tagggcaggc gccggttggc gaccatccag aaccggccgc cgggcttcag cgcggcggcg
     1081 gcgctgcgga taaagccctg acccagatcg tggcggtcgg cgcggtcgat atggaagggc
     1141 ggattgctga cgatgaagtc gtaacgctcc ggcaagcctt gcgtcgcgtc cagccagtga
     1201 aacgccgcgc gcgcgtccgg cacgttcgcg cgcgccagat cgagcgcgcg gccctctgcc
     1261 tcgatcaggt cgagccgcgt gatcgcgggc gaggcccgca gcgccacgtc gctcagataa
     1321 ccgatcccgg cgccgaggtc cgcgccgacg ccggcaaagg cgggcagatg cgcggccaga
     1381 aaccgcgacc ccgcgtcgag ccggtcccag gcgaacacgc cggggcggct cagataaccg
     1441 ctgtccaccg tgcgcggcgc gtcgagcgcg gcccattgcg cgatcaggcc gcgattcagc
     1501 cgcgccggat cgacctgcgc ccagaagacg cggcacttgt ttttcgacag gctggcgacg
     1561 ccgccgcaca gcgccttcag gtcgccttcg atggtcctgg cgccttcgag attggacgcg
     1621 caggcgatca cgacgccgcc cgccgccgtc tcatcgacgg tgcgcgccag cagggcgcga
     1681 tattcctcac gctga
//
LOCUS       DASVLL010000049         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_271581,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000049 DASVLL010000000
VERSION     DASVLL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271581"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /locus_tag="VF402_00850"
     CDS             <1..2025
                     /locus_tag="VF402_00850"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEX7798864.1"
                     /translation="TNAVTGGWRLSARGDLDSDAHYGGLDAAAKGRAARVPVTGSPGA
                     RQPEQPADAIVALDRAFDRFHLSARGLSLDLTADDAVSAPALRLRLKGGADAALNGGG
                     HLGLTADGALYDSHGRGGFTATLGGPGLPQAKLIVAGLTPEGGGDWTLSAQGDVAPVA
                     GARFDGHGHFASTQAGFGARLSEPAAFTAASAEIGDHLTGLSARLLPVAAPLLSVTAQ
                     GWRAEAGFDDLKLRAPNEQAGFAAGQGAFAAFAIPGADATGLKAGLTTGLVGDTLPAE
                     KKRFYPLEISGALTQDARVMTGRFTAATPNAKGADGKALPVAQIDLANGVASGKGTLK
                     IRTLGLDFAPGGLQPGALTPMTGAASQVTGKATFDGGFDWDAQTVTSGGTLTLDGLTF
                     LVGQTAARGLNGRIDFTSLSPLQSAPHQHLSVAAIDAGLPLSEVSADMQFLGDHVALE
                     DVHVTTPGGPVRLEPMDLPLDDKPMRGAVAFDGLDFGRIVAATSLSSSMSFEGRLSGR
                     LPFTIADGRATVSDGHVAADAPGRISIRRGSVSGVAATGDVTSENVAPATAALARANA
                     EAAVASDPNFNPFQDLAYQALEHLAYTRLEGTVNSHDGALEGGFTIKGYFDPPQKQKA
                     SISLFDYISGRWLKKPITLPSGTPIELHLDVPLGWDGDLFGLKPAADAEK"
     gene            2103..2282
                     /locus_tag="VF402_00855"
     CDS             2103..2282
                     /locus_tag="VF402_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEX7798865.1"
                     /translation="MKTVIAAAAALGALALAACTPTVNVNVNMSPIYAKIDVNVKVQL
                     EKDVQALAQQNPDLF"
     gene            2308..2694
                     /locus_tag="VF402_00860"
     CDS             2308..2694
                     /locus_tag="VF402_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEX7798866.1"
                     /translation="MMHLSSFKAVVAAGLLAVAAPGAVLVAAAPAFADIAAAKALVDG
                     AKAAGKVGELNTGYLGFVGAADSGALKAAVDEINAGRKQVYAQAAQKNGVSAEAAGIA
                     AFNTVILPKLASGDYYQDNSGKWARK"
BASE COUNT          502 a         1059 c         1057 g          435 t
ORIGIN      
        1 accaacgcag tcaccggcgg ctggcggctg agcgcgcggg gcgatctgga cagcgacgcc
       61 cattacggcg gcctcgacgc ggcggcgaaa ggccgcgccg cgcgcgtgcc ggtgaccgga
      121 tcgcccggag cgcgacaacc ggaacagccc gccgacgcca tcgtcgcgct cgatcgggcg
      181 ttcgaccgct tccacctgag cgcgcgcggc ctgagcctcg acctgacggc ggacgacgcc
      241 gtctcggcgc ccgccctgcg gctgcggctg aaaggcggcg ccgacgcggc gctgaacggc
      301 ggcggacatc tcggcctgac ggcggacggc gcgctttacg acagccacgg acgcggcggc
      361 ttcacggcga ccctgggcgg ccccggcctg ccgcaggcga agctgatcgt cgccggtctg
      421 acgcctgagg gcggcggcga ctggacgctg agcgcgcagg gcgacgtcgc gccggtggcg
      481 ggcgcgcgct tcgacggcca cggccatttc gcatccacgc aggccgggtt cggcgcccgc
      541 ctgagcgaac cggcggcctt cacggcggcg tcggcggaga tcggcgatca cctgaccggc
      601 ctgtcggccc gtctgctccc cgtcgcggcg cctttgctga gcgtcacggc gcagggctgg
      661 cgcgccgagg cgggtttcga cgatctgaaa ctgcgcgcgc ccaacgaaca ggcgggattc
      721 gcggcggggc agggcgcctt cgccgccttc gccattcccg gcgccgacgc gacgggcctc
      781 aaggccggcc tgacgacggg tctggtcggc gacacgctgc cggcggagaa aaaacgcttc
      841 tatccgctgg agatcagcgg cgccctgacg caggacgcgc gcgtcatgac cgggcgcttc
      901 accgccgcga ccccgaacgc caaaggcgcg gacggcaagg ccctgccggt ggcgcaaatc
      961 gacctcgcca acggcgtggc cagcggcaag ggaacgctga aaatccgcac gctgggcctc
     1021 gatttcgcgc cgggcggctt gcagccgggc gcgctgacgc cgatgaccgg cgccgccagt
     1081 caggtcaccg gcaaggcgac gttcgacggc ggcttcgact gggacgcgca gaccgtgacc
     1141 agcggcggga cgctgactct ggacggcctg accttcctcg tcggccagac ggcggcgcgc
     1201 ggcctgaacg ggcgcatcga cttcacctct ttgtcgccgc tgcaatcggc gccgcatcag
     1261 catctgagcg tcgccgccat cgacgcgggc ctgccgctca gcgaagtgag cgcggacatg
     1321 cagttcctcg gcgatcatgt cgcgctggag gacgtgcacg tgaccacgcc gggcgggccg
     1381 gtgcggctgg agccgatgga cctgccgctc gacgacaagc cgatgcgcgg cgcggtcgcc
     1441 ttcgacggcc tcgacttcgg tcgaatcgtc gccgccacca gcctgtcgtc cagcatgagt
     1501 ttcgagggcc gcctgagcgg tcgcttgccg ttcacgatcg cggacggccg ggcgacggtc
     1561 agcgacggcc atgtcgcggc ggacgcgccg gggcgcatct cgatccggcg cggctcggtc
     1621 agcggcgtcg ccgccaccgg cgacgtgacc agcgaaaacg tcgccccggc gacggcggcg
     1681 ctggccaggg cgaacgccga ggcggcggtc gcgtccgatc cgaacttcaa cccgtttcag
     1741 gatctggcct atcaggcgct cgaacatctg gcctacaccc ggctggaggg gacggtgaac
     1801 agccatgacg gcgcgctgga aggcggcttc accatcaagg gctatttcga tccgccgcaa
     1861 aagcagaaag ccagcatcag cctattcgac tatatcagcg gccggtggct gaaaaagccg
     1921 atcacgctgc cgtcgggcac gccgatcgag ctgcatctcg acgtgccgct gggctgggac
     1981 ggcgacctgt tcggcctgaa accggcggcg gacgcggaga aatgacgtaa gcggttccgt
     2041 tcagccccgg ttcagcctat atctgttgtc atgaacgcga agcaacggct gaggagcctt
     2101 agatgaaaac ggtaatcgcc gcggcggcgg cattgggcgc tctggcgctg gcggcctgca
     2161 cgcccacggt caacgtcaat gtcaacatgt cgccgatcta cgccaagatc gacgtcaacg
     2221 tgaaggtcca gctcgaaaag gacgttcagg ccctggcgca acagaacccc gaccttttct
     2281 gatttttaag caacagcgag acacgtcatg atgcatctgt cctccttcaa agccgtcgtg
     2341 gcggccgggc tgctggccgt cgccgcgccg ggcgccgtgc ttgtggccgc cgcgcccgcc
     2401 ttcgccgaca tcgccgccgc caaggcgctg gtcgacggcg ccaaggccgc cggcaaggtc
     2461 ggcgaactga acaccggcta tctcggcttc gtcggcgccg ccgattccgg cgcgctgaaa
     2521 gcggcggtcg acgagatcaa tgcgggccgc aagcaggtct atgcccaggc ggcgcagaag
     2581 aacggcgtct cggccgaagc cgccggcatc gccgccttca acacggtgat cctgccgaag
     2641 ctggcctccg gcgactatta tcaggacaac agcggcaagt gggcgcggaa gtagcggagt
     2701 atgtgaagct cccccttccg tcaccgcctc cggcgacgct gccttccccg tgaacgggga
     2761 aggcgggagt ccttattcgc cctccctgcg aagcggggag gtgggaacgc cgaaggcggt
     2821 gggaggggcc ttcccataga cgacggagga acgcccaacg gcataattcc tgcaccgccg
     2881 ggatgaatcg cgtaaacccg gcgccagatt ggaacagaca tgccgcagga ccacctcaga
     2941 ccgctcacca tcctgcgttt cttcgccgct gcctgggtcg ttctgtacac ctatatccac
     3001 gaattgaagg gcgccttctc catcggcctg ctggacaagg gctatctcgg cgt
//
LOCUS       DASVLL010000050         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_217404,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000050 DASVLL010000000
VERSION     DASVLL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217404"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="VF402_00865"
     CDS             <1..1559
                     /locus_tag="VF402_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7798867.1"
                     /translation="LGHLFNLDAAQIEGVDSYFRIHRPRRVRQPGEFSSYSNFGVALA
                     ARALVQTSHAKDAPSLIEARVFGPLGMAHTTLREPYQPNNLNVYDLPAPLSPQLTQGL
                     SDGFVWDGATYSPQPFDHALPLSGALGASSTAQDMAVFMSMMLSNGQYGGVQLFNADS
                     ARAFRTPMLKVPEGYNGWASGLMVRAAPSGEITYGHGGATLWFNANMVLVPSLNLGVY
                     IAANTQTGGPLTQSFPNLLLDHLSGDLVRPPLMPSPDMAYADHRDYYRAISGQYVSTR
                     RAYGGLEGAVTRLLNTVSVSVDRDGRLILATADGLSAFVPTPAPGFFAQQDAEDPGPA
                     METGGLHFLLSADGRARAFETASNLARYERVGWLHSPQTLGWLSVVTVVLSLLTLLSL
                     ARPRQRHEQPTRAQGWATLVSVGMAVLWLLALFIFHSWRAGLADNPGALFTRWPSGSV
                     RLASGLAFLAALGALYQAASLYFVLADQARYGEGWNAWQKMAHAALVAWSLFYAVVLM
                     LWGALEPWSW"
BASE COUNT          234 a          628 c          559 g          297 t
ORIGIN      
        1 gcctcggcca cctgttcaac ctcgacgccg cgcagatcga aggcgtcgat tcctatttcc
       61 gcatccaccg cccgcgccgc gtgcgccagc cgggcgaatt ttcgtcctac tccaatttcg
      121 gcgtggcgct ggcggcgcgc gctctggtgc agacctcgca cgccaaggac gcgccgtcgc
      181 tgatagaggc gcgcgtcttc ggcccgctgg gcatggcgca caccacgctg cgcgagccgt
      241 accagccgaa caatctcaat gtctacgacc tgcccgcgcc cctgtcgccg caactgacgc
      301 aaggcctgtc ggacggcttc gtctgggacg gcgccaccta ttcgccgcag cccttcgacc
      361 acgccctgcc gctgtccggc gcgctgggcg cctcatcgac cgcgcaggac atggccgtct
      421 tcatgtcgat gatgctcagc aacggccagt atggcggcgt gcaactgttc aacgccgatt
      481 cggcgcgcgc cttccgcacg ccgatgctga aggttcccga aggctataac ggctgggcgt
      541 ccggcctgat ggtccgcgcc gcgccgtcgg gcgagatcac ctacggccac ggcggcgcga
      601 ccctgtggtt caacgccaat atggtgctgg tccccagcct caatctcggc gtctatatcg
      661 ccgccaacac ccagaccggc gggccgctga cgcaaagctt ccccaacctg ctgctcgatc
      721 atctgagcgg cgatctggtc cggccgccgc tcatgccctc acccgacatg gcctatgccg
      781 atcaccgcga ctattaccgc gcgatcagcg ggcagtacgt ctcgacgcgc cgcgcctatg
      841 gcggactgga aggcgcggtg acgcggcttc tgaacaccgt ctcggtcagc gtcgaccgcg
      901 acgggcggct gatcctggcc accgccgacg ggctgtcggc cttcgtgccg acccccgcgc
      961 ccggcttctt cgcgcagcag gacgccgagg atcccggccc ggctatggag accggcggcc
     1021 tgcatttcct gctgtccgcc gacggccggg ccagggcgtt cgagacggcc agcaatctgg
     1081 cgcgttacga gcgcgtcggc tggctgcaca gcccgcaaac cctgggctgg ctcagcgtcg
     1141 tgaccgtcgt gctgtcgctg ctgaccctgt tgtcgctggc gcgcccgcgt cagcgccacg
     1201 aacagccgac gcgggcgcag ggctgggcca cgctcgtctc ggtcggcatg gcggtcctgt
     1261 ggctgctggc cctgttcatc ttccatagct ggcgcgcagg gctggccgac aatccgggcg
     1321 ccctgttcac gcgctggccg tcgggctcgg tgcggctggc ctccggcctg gccttcctcg
     1381 ccgcgctcgg cgcgctgtat caggccgcca gcctctattt cgtgctggcc gatcaggcgc
     1441 gctacggcga gggctggaac gcgtggcaga aaatggcgca cgcggccctc gtcgcgtggt
     1501 cgctgttcta cgccgtcgtg ctgatgctgt ggggcgcgct ggagccctgg agctggtagg
     1561 gcctctcagc gcaccagcga atagctgttg acgaagccgt atttcgcaat cgccgtccgc
     1621 acctccggcc gggccagcat ccgatgcaaa gcggcgcgca tccgcgcctc atagggcgtc
     1681 tgatgggccg gttcgatata ggtctgcgcc tcgccgag
//
LOCUS       DASVLL010000051         6530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_110321,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000051 DASVLL010000000
VERSION     DASVLL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110321"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VF402_00870"
     CDS             <1..642
                     /locus_tag="VF402_00870"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7798868.1"
                     /translation="HFRVEPLKKLGGWDAFNVTEDADLGVRLAAEGYESGLLRSPTGE
                     TPTGSSRAWLPQRTRWIKGYMQTLLVHTRGVKRGARVWAGLGLGVGMSVLAALAYAPL
                     SVMMLATLLLFGLQALGDPARAVEAPGMADLGLFVFGNAAAMYAMIVGQRRAGLKVRV
                     RDVLSAPFYWSLQTLAAAFALWQLFVRPFHWDKTEHAPAAGEGCQSLPMADEV"
     gene            complement(951..1893)
                     /locus_tag="VF402_00875"
     CDS             complement(join(951..1556,1558..1893))
                     /locus_tag="VF402_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7798869.1"
                     /translation="MTRPYSEDLRERALARADAGETVRDIAAALHISPSCVSKWKTLR
                     RETGSISPGKIGGHKSRTLSGATADWLRERMASAPFTTRQLAAELAERSIKTTRRAVW
                     VFVHDEGLSFKKTIYPTEQDRPDVARKRLRWKDHQGQVDPQRLVFIDETWVKTNMAPL
                     WGWAPIGRRLHGKAPYGHWKTMTFIAALRHDRIDAPFVFDGPINAELFTLYVHQILAP
                     TLGQGDIVVLDNLGSHKSKAVRRAIHDRGAHLLFLPPYSPDLNPIEQVFAKVKHFMRK
                     AEERTVEATWKKVGQVLDLFQPNECLNYIRNSGYVSV"
     gene            complement(2256..3119)
                     /locus_tag="VF402_00880"
     CDS             complement(2256..3119)
                     /locus_tag="VF402_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX7798870.1"
                     /translation="MSKSMTKLAIGLIAAGALLTGGATLAESNAAPLVQPPHPFTFEG
                     PFGTFDQAQLQRGYKVYREVCSTCHAMHLMSFRNLGQKGGPFYDPKYKNPNDNPVVKE
                     IASEYDIADIDPDTGDDITRKGTPADRFPSPFKNDEAAKASNNGALPPDMSVLAKARE
                     GGALYIYNVVSGSHTPPAGLELNPGQYYYPHVNGSLRALWKGDPAHVPHGGVLAMPPP
                     LKSNGQVTYDDGTPSTVQQNAEDVATFLAWASDPHAVERKQTGFAVIIYLLLLAGLTY
                     AAYRQVWRGKH"
     gene            complement(3125..4432)
                     /locus_tag="VF402_00885"
     CDS             complement(3125..4432)
                     /locus_tag="VF402_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX7798871.1"
                     /translation="MSEHASTYQPRSGIEKWLDSRLPIVRLAHDSFVDYPTPKNLNYW
                     WTFGGILSMCLALQIVTGVIMAMHYTANSALAFDSVERIMRDVNYGWLLRSAHANGAS
                     MFFFAVYIHMFRGLYYGSYKAPREVLWLLGCIIYFLMIATAFMGYVLPWGQMSYYGAV
                     VITNLIGAIPVVGPSILTWLQGGFAVDNPTLTRFFSLHYLLPFVIAGVVVLHVWALHV
                     VGQNNPTGVEIKSKEDTVPFTPYATVKDGLGMVVFLIMFAVFVFFAPDALGHSDNYIP
                     ANPLQTPAHIVPEWYMLPFYAILRAITFDIGPIPAKLIGVVLMFTAIAAIFLLPWLDT
                     SKVKSMRYRPVMRWFFFLFVVACLGLGWCGAQEPAQIVFKFGVDKLGEPTGVNVTTVA
                     QVLAAYYFAFFFIIMPWIGLKEEPLPVPASISEAVLAAHAKKA"
     gene            complement(4435..4971)
                     /gene="petA"
                     /locus_tag="VF402_00890"
     CDS             complement(4435..4971)
                     /gene="petA"
                     /locus_tag="VF402_00890"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX7798872.1"
                     /translation="MSEPVLDGGTLDPNRRDFIYIAAGAGAAGAAAMALIPLIGQMNP
                     AADTLALASIEYDLSKVVEGQQVVVKWRGKPLFVRYRTPKEIKEAQDTDWKTLREPQP
                     DSARVKAGHEQYLILVGVCTHLGCVPNFGGGEYGGWLCPCHGSVYDTSGRIRQGPAPK
                     NLEVPDYAFLTDSKIKVG"
     gene            5366..5836
                     /locus_tag="VF402_00895"
     CDS             5366..5836
                     /locus_tag="VF402_00895"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX7798873.1"
                     /translation="MRLALYQPDIPQNLGAALRLSAAFAVELDVIEPCGFPLGDKAIR
                     RAAMDYAACAKVSHHASWSAYLAFAAALPDPPRLVLMTTKGARPLYDFTFRPGDVLLM
                     GRESAGVPDDVHERADARLYIPMSAQARSINVINAAAMALGEGLRQLHGFPGQA"
     gene            5854..>6530
                     /locus_tag="VF402_00900"
     CDS             5854..>6530
                     /locus_tag="VF402_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX7798874.1"
                     /translation="MTDTDLIDARRQQAAQWFRRLRDDICAAFEALEDAAAPELYGEG
                     AGRFERTPWSRPEGGVKGAGGGGEMSIMKGRLFEKVGVHISTVHGEFKPEFARSIPGA
                     AEDPRFFATGISLIAHMTNPHVPAVHMNTRFITTTQSWFGGGADLTPLLAGDRREDAP
                     DAVAFHAALKAACDAHDPAYYERFKAWCDDYFYLPHRDEPRGVGGVFYDHLDSGDHAA
                     DFAFTQDV"
BASE COUNT         1170 a         2024 c         2146 g         1190 t
ORIGIN      
        1 catttccgcg tcgagccgct gaagaagctg ggcggatggg acgcgttcaa cgtcaccgag
       61 gacgccgatc tgggcgtgcg gctggcggcg gagggctatg agagcggcct gttgcgcagc
      121 ccgaccggcg agacgccgac cgggtccagc cgcgcctggc tgccgcagcg cacgcgctgg
      181 atcaaggggt atatgcagac gctattggtg catacgcgcg gcgtgaagcg cggcgcaagg
      241 gtgtgggccg gattgggcct cggcgtcggc atgagcgtgc tggcggcgct ggcctatgcg
      301 ccgctgagcg tgatgatgct ggcgaccctg ctcctgttcg gcttgcaggc tttgggcgat
      361 ccggcgcggg cggtcgaggc accggggatg gccgatctgg ggctgttcgt gttcggcaac
      421 gcggcggcga tgtatgcgat gatcgtcggg cagaggcggg cggggctgaa ggtgcgcgtc
      481 cgcgacgtgc tgagcgcgcc gttctactgg agtttgcaga ccctggcggc ggcgttcgcg
      541 ctctggcaac tgttcgtgcg gccgttccac tgggacaaga ccgaacacgc gccggcggcg
      601 ggcgagggct gccagagcct gccgatggcg gacgaggttt agggcgtttc cccaaaaagc
      661 agctccactt tttggataag gaatgtctat aaaacaaagg cttagagcgc cgatctgatc
      721 ccatcggatc gttgctccag ctaaagcggc gtgacggagg cgcaaaacgg taaggctaaa
      781 ccggcgcggc gttggggcgg ctgagggcgg agccgcaggt ttttatccac agatggacac
      841 agatgttcgg ggtggatttc acgctaccgc tatcggcgga ggaaggttgt tatcgcggat
      901 gttggccttt ggctatctgc acggggcgcg ctaactagag catgcctcct ttacacggaa
      961 acataccctg agtttctaat gtagttgagg cattcgttcg gttggaagag gtcgagtacc
     1021 tgaccgacct tcttccatgt ggcctcgacc gtgcgctctt cagccttccg catgaagtgt
     1081 ttgacctttg cgaagacctg ttcgatggga ttcaggtcgg ggctgtaggg cggcagaaag
     1141 agcaggtgcg cacctctatc gtggattgcc ctgcgaacgg cctttgattt gtggctcccg
     1201 agattatcga gcacgacgat gtcgccttgg ccaagcgttg gcgccaggat ttgatgtacg
     1261 tagagagtga acagctcggc attgatgggg ccgtcgaaaa cgaagggggc gtcaatccga
     1321 tcatgccgca aggcggcgat gaaggtcatg gtcttccagt ggccgtaggg ggccttgccg
     1381 tgcaggcgcc gacctatggg cgcccagccc catagcggcg ccatattcgt tttaacccag
     1441 gtttcatcga tgaagaccaa gcgctgcgga tcaacttgcc cttggtgatc cttccatcgc
     1501 agccgttttc gagcgacgtc gggacgatcc tgctcagtcg gataaatcgt tttttttgaa
     1561 gctgaggcct tcgtcgtgga cgaaaaccca gacagcccgc cgcgtcgtct taatgctccg
     1621 ctcagccagc tcggcagcca gttggcgcgt cgtaaacggg gctgacgcca tacgctcgcg
     1681 aagccagtcg gctgtcgctc cagacagggt gcgagattta tggccaccga tcttgcccgg
     1741 agaaatactg cccgtttccc gtctcaacgt cttccacttc gaaacgcacg aagggctgat
     1801 atgaagcgca gcggctatat cgcgaacggt ctcacccgca tcggcgcgcg caagcgcacg
     1861 ctctcgcaaa tcttccgaat aaggtcgtgt catgacaact ggcctcgcta cccagcaatc
     1921 agcttgaatc acgacacgac tgatttggga atcccgattc cgctaaaaaa gagcacgctc
     1981 tagggacaag ccccggaacc ctgagccaaa actgcaaaac ccgccggatc gcttcggcgg
     2041 gttttctgtt tctggtcttc cccgtttacg gggaaggtgg attttggcgg gcgccgcccg
     2101 tcgaaagacg gaagagggac agcggcgcca tcccccctcc accgcttcgc ggtcccatgg
     2161 atttgcaaaa gctatacttt tgcttcgcca aacccccgta aacgggggag gatatagtcg
     2221 tagcgccgcg agatgcggct tgctgcccgc ccccgtcagt gcttcccgcg ccagacctga
     2281 cggtaggcgg cataggtgag tccggccagc agcagcagat agatgatcac cgcgaagccg
     2341 gtctgcttgc gctcgaccgc gtgcgggtcg gaggcccagg ccaggaaggt cgccacgtcc
     2401 tcggcgttct gctgcacggt cgacggcgtg ccgtcgtcat aggtgacctg cccattcgac
     2461 ttcagcgggg gcggcatggc cagcacgccg ccgtgcggca cgtgcgccgg gtcgcccttc
     2521 catagcgcgc gcaggctgcc gttgacgtgc ggatagtagt actggcccgg attcagctcc
     2581 agacccgccg gaggcgtatg cgagccgctg acgacgttgt agatgtacag cgccccgccc
     2641 tcgcgcgcct tggccagcac cgacatgtcg ggcgggaggg cgccgttgtt ggacgccttg
     2701 gccgcttcgt cgttcttgaa cggcgacggg aagcggtcgg cgggcgtgcc cttgcgggtg
     2761 atgtcgtcgc cggtgtcggg atcgatatcg gcgatgtcgt attcggaggc gatctccttc
     2821 accaccggat tgtcgttcgg gttcttgtac ttcggatcgt agaagggccc gcccttctga
     2881 cccagattgc ggaacgacat caggtgcatc gcgtggcagg tcgagcagac ctcgcgatag
     2941 accttgtagc cgcgctgcaa ctgcgcctga tcgaaggttc cgaacggccc ctcgaaggtg
     3001 aagggatgcg gcggctggac caggggcgcg gcgttcgatt cggccagagt tgcgccgccc
     3061 gtcagcaagg cgcccgccgc gatcaggcca atcgccagtt tcgtcatgga tttcgacatg
     3121 gacatcaagc cttcttcgcg tgggcggcca aaacggcttc ggaaatggag gccgggaccg
     3181 gcaggggttc ttctttcagg ccgatccacg gcatgatgat gaagaagaaa gcgaaatagt
     3241 aggcggccag aacctgcgcg accgtggtca cgttgacgcc ggtcggctca cccagcttgt
     3301 cgacgccgaa cttgaacacg atctgcgccg gctcctgcgc gccgcaccag ccgaggccga
     3361 ggcaggcgac cacgaacagg aagaagaacc agcgcatcac cgggcggtag cgcatcgact
     3421 tgaccttgga ggtgtccagc cacggcagca ggaagatggc cgcgatggcg gtgaacatca
     3481 gcaccacgcc gatcagcttg gccgggatcg gcccgatgtc gaaggtgatc gcgcgcagga
     3541 tcgcgtagaa gggcagcatg taccattcgg gcacgatgtg cgcgggcgtt tgcagcgggt
     3601 tggccgggat atagttgtcg ctgtggccca gcgcgtccgg cgcgaagaag acgaagacgg
     3661 cgaacatgat caggaacacc accatgccga ggccgtcctt gaccgtcgcg tagggcgtga
     3721 acggaaccgt gtcctccttc gacttgatct cgacgccggt cggattgttc tggcccacca
     3781 cgtgcagcgc ccagacgtgc agcacgacga cgcccgcgat cacgaacggc agcagatagt
     3841 gcagcgagaa gaagcgcgtc agggtcggat tgtcgaccgc gaagccgcct tgcagccagg
     3901 tcaggatcga cggcccgacg accggaatgg cgccgatcag gttggtgatc accaccgcgc
     3961 cgtaatagga catctggccc cacggcagca catagcccat gaaggccgtg gcgatcatca
     4021 ggaagtagat gatgcagccc aggagccaca gcacttcgcg cggcgccttg tacgagccgt
     4081 aatagaggcc gcggaacatg tggatataga cggcgaagaa gaacatcgac gcgccgttgg
     4141 cgtgggccga gcgcagaagc cagccgtaat tgacgtcgcg catgatgcgt tcgaccgagt
     4201 cgaaggccag cgccgaattg gcggtgtagt gcatcgccat gatcacgccg gtgacgatct
     4261 gcagggccag gcacatcgac aggatgccgc cgaaggtcca ccagtagttc aggttcttcg
     4321 gcgtcggata atcgacgaag ctgtcatggg ccaggcgcac gatcggcagg cgcgaatcca
     4381 gccatttctc gatgcccgat ctgggctgat aggttgaagc atgctcgctc atctttagcc
     4441 caccttgatc ttgctgtcgg tcaggaaggc gtagtccggc acttccagat tcttcggcgc
     4501 cggtccctga cggatgcggc cggaggtgtc gtacaccgag ccgtggcacg ggcagagcca
     4561 gccgccgtat tcgccgccgc cgaaattggg cacgcagccg aggtgggtgc agacgccgac
     4621 caggatcagg tactgctcat gaccggcctt gacgcgggcg gagtcgggct gcggttcgcg
     4681 cagggttttc cagtccgtat cctgggcttc cttgatttcc ttgggggtgc gatagcggac
     4741 gaacaaaggc ttgccgcgcc atttgacgac gacctgctgg ccctcgacga ccttggacag
     4801 gtcgtattcg atcgacgcca gcgccagcgt gtcggcggcc ggattcatct gtccgatcag
     4861 cgggatcagc gccatcgccg ccgcgcccgc ggcgcccgcc cccgccgcta tatagatgaa
     4921 atcgcgacgg ttgggatcga gtgtcccgcc gtccagtaca ggttcgctca tattggcgcc
     4981 tctccatgtc catacgccgg gcgcccaggc ttgtctccgc ctgcctccgc tgcgcccgcc
     5041 ggtccgcgcg gcgcgcgacc ggtcacttta gtttcctccg ccggtccccg tgcgaaacgc
     5101 ggccggcgct ttttcgattc gttgttaagg gaacgtgatt ccgtaaccgt gttaagcctt
     5161 tactgcggcg acctgtcgcg gcttacgtcc ctaccgtcta taatcacatt tccacgaagc
     5221 tgacaaaccg gcaaacactt tctcgcgccg attcccgcca taattgacgt accggcgaga
     5281 tttatcgctg cggcgctttc gctctggacg gcggcggggc cgggcggtta aagcgcgccg
     5341 actttcccca tcggaggccg cgatgatgcg cctggcgctc taccaacccg atattccgca
     5401 aaacctcggc gcggccctgc ggctcagcgc cgccttcgcg gtcgaactgg acgtgatcga
     5461 gccgtgcggc tttccgctgg gcgacaaggc gatccggcgc gcggccatgg attacgcggc
     5521 ctgcgccaaa gtgtcgcacc atgcgtcgtg gagcgcctat ctggccttcg ccgccgccct
     5581 gcccgatccg ccgcgcctcg tgctgatgac cacgaagggg gcgcggccgc tctacgactt
     5641 tacgttccgg cccggcgacg tcctgctgat gggccgcgaa agcgcgggcg ttcccgacga
     5701 cgtgcatgag cgcgccgatg cgcgtcttta tattccgatg agcgcgcagg cgcgctcgat
     5761 caacgtgatc aacgccgccg ccatggcgct gggcgaaggg ctgcgacaat tgcacggctt
     5821 ccccggacag gcctgagcgc gtatagccgg aacatgaccg acaccgacct tatcgacgcc
     5881 cgccgccaac aggccgccca atggttccgc cgactgcgcg acgacatctg cgccgcgttc
     5941 gaggcgctgg aggacgccgc cgcgcccgaa ctgtacggcg aaggggcggg ccgtttcgag
     6001 cgcacgccgt ggtcgcgccc cgaagggggt gttaaaggcg caggaggggg cggcgagatg
     6061 tcgatcatga agggccgcct gttcgagaag gtcggcgtgc atatctccac cgtgcacggc
     6121 gaattcaaac cggaattcgc cagatcgatt ccgggcgccg ccgaggatcc gcgtttcttc
     6181 gccacgggga tcagcctgat cgcgcacatg accaatccgc acgtccccgc cgtgcatatg
     6241 aacacgcgct tcatcaccac gacgcaaagc tggttcggcg gcggggcgga cctgacgccg
     6301 ctgctggccg gcgaccggcg cgaggacgcg cccgacgccg tcgcgtttca cgccgcgctg
     6361 aaggccgcct gcgacgcgca cgatccggcc tattacgagc gcttcaaggc gtggtgcgac
     6421 gactatttct acctgcccca ccgcgacgag ccgcgcggcg tcggcggcgt cttctacgac
     6481 cacctcgatt ccggcgacca cgccgccgac ttcgccttca cgcaggacgt
//
LOCUS       DASVLL010000052         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_56295, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000052 DASVLL010000000
VERSION     DASVLL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56295"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF402_00905"
     CDS             complement(<1..291)
                     /locus_tag="VF402_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX7798875.1"
                     /translation="MRTAIDFSPLYRSAVGFDRLVSLLDSATATSASAPGWPPYNIER
                     LAKETEDQGDSYRIEIAVAGFRPEQLNIEVKENVLTVAGKKDADEANRTFLHR"
     gene            complement(434..1480)
                     /gene="trxB"
                     /locus_tag="VF402_00910"
     CDS             complement(434..1480)
                     /gene="trxB"
                     /locus_tag="VF402_00910"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7798876.1"
                     /translation="MPMSAPRPVRCAIIGTGPAGWTAAIYAARGLLAPVVFTGPQDGG
                     QLTITTDVENYPGFAEVVQGPWLMDQMRAQALHMGAEVIGEIVTTADLSQRPFLLQTE
                     SGAKFLAETVIIATGAQAKWLGLESEKTYQGFGVSACATCDGFFYRNKVVAVVGGGNT
                     AVEEALFLTKFASRVYLIHRRDTLRAEKILVERMLAEPKIEPIWNVAIDEILGETNAF
                     GGAAVTGVRLQDVHTQQHRVIDLDGVFIAIGHAPASSLFEGQLETKQGGYLVVEPGTP
                     KTAIKGVYAAGDVTDDVYRQAVTAAGMGCQAALEAVRLLAEEDHHRAQLTACEVAEEF
                     SHLTLDQRKAATKS"
     gene            complement(1691..>2160)
                     /locus_tag="VF402_00915"
     CDS             complement(1691..>2160)
                     /locus_tag="VF402_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HEX7798877.1"
                     /translation="RAFDLDADHAVLIARDVRAAIEAAGGSVLLTVSRRTPPEARAVL
                     TDGLRALPGMVYDGTGDNPYFAFLHAADRFLVTEDSVNMATEAAATGKPVQIVSLPRR
                     TLGGGAKFEDFHESLRARGVAHPFETHLSGDPYPPLNETARAAQILLERYRKR"
BASE COUNT          337 a          672 c          757 g          394 t
ORIGIN      
        1 gcgatgcagg aaggtgcggt tcgcttcgtc ggcgtccttc ttgccggcga cggtcagcac
       61 gttttccttc acctcgatat tgagctgttc gggccggaag ccggcgacgg cgatttcgat
      121 gcggtaggaa tcgccctgat cttcggtttc cttggcgagg cgctcgatat tatagggcgg
      181 ccagccgggc gccgaggcgc tggtggcggt cgcgctgtcg agcaggctga ccagacggtc
      241 gaagccgacg gcggaacggt acaggggcga gaaatcgata gcggtgcgca taagggctct
      301 ccttaatata agcgaggcct tgcagggccg tcatcgacgg ggcgtgcctg caaaggtttg
      361 attttgaacg gcgtccacat ccccaaatgg gcgatgtcgc cgtccaagaa ggcccgctcg
      421 gcggcgcctt cgcctatgat ttggtggccg ctttgcgctg gtcaagggtc aggtgcgaaa
      481 attcttcggc gacctcgcac gcggtcagtt gcgcgcggtg gtggtcctcc tcggccagca
      541 ggcgtacggc ttccagcgcg gcctgacagc ccatgcccgc cgccgtcacc gcctgacgat
      601 agacgtcgtc ggtgacgtcg cccgccgcat agacgccctt gatcgcggtt ttgggcgtgc
      661 ccggctcgac gacgagatag ccgccttgct tggtttccaa ctggccctcg aacaggctgg
      721 aggccggggc gtggccgatg gcgatgaaca cgccgtcgag gtcgatgacg cgatgctgct
      781 gcgtatgcac gtcctgaagg cgcacgccgg tgaccgccgc gccgccgaag gcgttggttt
      841 cgccgaggat ttcatcgatg gcgacgttcc atatcggctc gatcttcggt tcggccagca
      901 tgcgctcgac cagaatcttt tcggcgcgca gcgtgtcgcg gcggtggatc agatagaccc
      961 tggaggcgaa cttggtcagg aacagcgcct cctcgaccgc cgtattgccg ccgccgacca
     1021 cggccacgac cttgttgcgg taaaagaagc cgtcgcaggt ggcgcaggcc gacacgccga
     1081 agccctgata ggtcttttcc gattcgaggc ccagccattt ggcttgcgcg ccggtggcga
     1141 tgatgacggt ctcggctagg aatttcgcgc cgctttcggt ttgcagcagg aacgggcgct
     1201 gactgagatc ggcggtggtg acgatctcgc cgatcacttc ggcgcccatg tgcagcgcct
     1261 gggcgcgcat ctggtccatc agccacggcc cctgcaccac ctcggcgaag ccgggatagt
     1321 tctcgacgtc ggtggtgatg gtgagctggc cgccgtcctg cgggccggtg aacaccaccg
     1381 gcgccagaag gccgcgcgcg gcgtagatgg cggcggtcca gccggcggga ccggtgccga
     1441 taatggcgca gcgaacggga cggggagcag acatgggcat acctggaatc ggaggtctta
     1501 acggctccct atgtagggag cgggcggcgg tttgtcacct cgaaagcgcg cctttcgcca
     1561 accttcccct ctcccggcct gcggggccga gtaggtttgc gctcgactgg ctggcggccc
     1621 ctcacccgat cgccttcgct gacgctcggc tctcgtcctc tcccctcatg gaggggagag
     1681 ggggaagggt ttatcttttc cggtagcgtt ccagcaggat ttgcgccgcg cgcgccgttt
     1741 cgttgagcgg cgggtaggga tcgccgctca gatgcgtctc gaacggatgc gcgacgccgc
     1801 gcgcgcgcaa ggattcgtgg aaatcctcga acttggcgcc gccgccgagg gtgcggcggg
     1861 gcagggagac gatctgcacc ggcttgcccg tcgcggcggc ctctgtggcc atattgaccg
     1921 aatcctcggt gaccagaaag cggtcggcgg cgtgcaaaaa ggcgaaatag ggattgtcgc
     1981 ccgtcccgtc atacaccatg ccgggcaggg cgcgtaagcc gtccgtcagc acggcgcgcg
     2041 cttcgggcgg cgtgcggcgc gagacggtca gcagcacgct gccgcccgcc gcctctatgg
     2101 ccgcacggac gtcgcgcgcg atcaatacgg catggtcggc gtcgagatcg aaggcgcggg
//
LOCUS       DASVLL010000053         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_271867,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000053 DASVLL010000000
VERSION     DASVLL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271867"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1642)
                     /locus_tag="VF402_00920"
     CDS             complement(56..1642)
                     /locus_tag="VF402_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798878.1"
                     /translation="MISTIRRGLAALIAIAAICAPAARAADWIEAQSAHFTIYSQAPE
                     KKTRLYVRKLEAFHTLTNMLLGASDNGPQTRFTVYLLADNDQMLLVRPNFARQVGGVY
                     FNCAEGTQAYASLQYFDMNDQDPSLVTLLHEYSHYVMFQHARTWYPAWYVEGFAEYLS
                     TADPEKGSITVGEMSQERDFTLSQDRWIAFDKVLDPDFGFAGDKSNNPWEIESFYAQS
                     WLLTHYMLSDDGRARALNAYFARLQNGEAPVAAFEAATGIKVATLESTLKRYAQKTFY
                     LKVPVPDYPDAQIAVTPVPAADAAWLFDASLLTTCPDATQGKAILWRLTALDTPQASV
                     GLRLAVDRARILYGDAAAAAADDLKALTASTPDSFDANYLLGRAYLKQARDKSGDEAR
                     ALTDQARASFLAAYRLKKLDAPNLYFLASSFSDQPGFPDMNVVNAAGGAHVLAPGVLD
                     YAGLAAFADLINDRRDAAVAALEPFIGDPHNRQQAERVKDTVDAIRAGKTTPEIMRIM
                     NGGGPEPTTPALPEKPKDQK"
     gene            complement(1730..2059)
                     /gene="grxD"
                     /locus_tag="VF402_00925"
     CDS             complement(1730..2059)
                     /gene="grxD"
                     /locus_tag="VF402_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX7798879.1"
                     /translation="MTDDVNTFIDDTIKGHDVVLFMKGVPEQPRCGFSGLVVQVLDHL
                     GVDYVGVDVLQDESLREGIKLYSDWPTIPQLYVKGEFIGGADIVREMFQSGELKQTLS
                     EKGLIEA"
     gene            complement(2160..3068)
                     /locus_tag="VF402_00930"
     CDS             complement(2160..3068)
                     /locus_tag="VF402_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7798880.1"
                     /translation="MRYRPFGRSGLALSTIGLRLSRLKIGGNVGLLNKLIMAGLENGV
                     NTYHFDTTAPDFLRAAAQAFSVVERRLLFVSADAQEAGQACDSTGYALRPLKERLRAA
                     IKDTGLQALDLLMFSPPGALQLPADTLSFLDSLKRARMLRYIGARGDAGDMALLIADG
                     HFNVIETSFDIDSTWDKRHQIDRAVQSEITVIGGDFFPDAYRKASDVVPKAARRGWFG
                     ARPENPLAGAGTHAFLHTTPDWTPQELCLGYALAQPSLSCVFIDAETPQQIEALCAVS
                     ERALPSSVPAQIEMARFNDQIGAARR"
     gene            3335..>3625
                     /locus_tag="VF402_00935"
     CDS             3335..>3625
                     /locus_tag="VF402_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798881.1"
                     /translation="MRRFGFLLALAVMAQPAQACPDPAAALDSALAATIAAVRGGDAQ
                     ALLGRMSAEGVSFGPDAPPTPLAVLRAQFADRGGRYCDVFACNGVEGALHRRF"
BASE COUNT          583 a         1131 c         1286 g          625 t
ORIGIN      
        1 atcgcgccca gggtcatggc tgcgcgcgcg cgaaaactgt gggacatgcg catggctatt
       61 tctgatcctt cggtttttcc ggcagggcgg gcgtcgtcgg ctccgggccg ccgccgttca
      121 tgatgcgcat gatttcgggc gtggtcttgc ccgcccggat ggcgtcgacc gtatccttga
      181 cgcgctcggc ctgctggcgg ttgtgcgggt cgccgatgaa cggctccagc gccgccaccg
      241 ccgcgtcgcg ccggtcgttg atcaggtcgg cgaaggccgc cagaccggcg tagtcgagca
      301 cgccgggcgc cagcacatgc gcgccgcccg ccgcattgac cacgttcatg tcgggaaagc
      361 ccggctggtc ggaaaagctg ctggccagaa aatacaggtt gggcgcgtcg agctttttca
      421 gcctgtaggc ggccagaaag gacgcgcgcg cctgatcggt gagggcgcgg gcctcatcgc
      481 cggacttgtc ccgcgcctgt ttcagatagg cgcggcccag caggtagttg gcgtcgaagc
      541 tgtcgggcgt gctggcggtc agcgccttga gatcgtccgc cgcggcggcg gccgcgtcgc
      601 cgtagagaat ccgcgcgcgg tcgacggcca gccgcaggcc gacggacgcc tgcggcgtat
      661 cgagcgccgt cagccgccac agaatggcct tgccctgagt cgcgtcgggg caggtggtca
      721 gcagcgaggc gtcgaacagc cacgccgcgt cggcggccgg aaccggcgtg acggcgatct
      781 gcgcgtcggg atagtcgggg accggaacct tcaggtaaaa ggtcttttgc gcatagcgtt
      841 tcagcgtcga ttccagcgtc gcgaccttga tcccggtcgc cgcctcgaag gccgccaccg
      901 gcgcctcgcc gttctgcaag cgcgcgaaat aggcgttcag cgccctggcc cggccgtcgt
      961 cgctgagcat gtagtgggtc agcagccatg attgcgcgta gaagctttcg atctcccacg
     1021 gattgttgct cttgtcgccc gcgaagccga aatcggggtc cagcaccttg tcgaacgcga
     1081 tccagcggtc ctgcgacagg gtgaagtcgc gctcctgcga catttcgccg acggtgatgg
     1141 agcctttttc gggatcggcg gtggacaggt attcggcgaa gccctcgacg taccaggccg
     1201 gataccaggt ccgggcgtgc tggaacatga cgtaatggct gtattcgtgc agcagggtga
     1261 ccaggctggg atcctgatcg ttcatgtcga aatattgcag gctggcatag gcctgcgtgc
     1321 cttcggcgca gttgaaatag acgccgccga cctgcctggc gaagttgggc cgcaccagca
     1381 gcatctggtc gttgtcggcc agcagataga cggtgaagcg cgtctgcggc ccgttgtccg
     1441 acgcgcccag cagcatgttg gtcagggtgt ggaaggcttc gagcttgcgc acatagaggc
     1501 gcgtcttctt ttccggcgcc tggctgtaga tggtgaaatg cgccgactgc gcctcgatcc
     1561 agtcggcggc gcgcgcggcg ggcgcgcata tcgctgcgat ggcgataagc gcggccaggc
     1621 cgcgccggat cgtggatatc atgaaagcct ccccctttat cgcgggggag gataggacaa
     1681 tccgggaaat ctggaaaggg ggatcaggcc ctcagatcag gccctcagat caagcctcga
     1741 tcaggccctt ttcgctcagg gtctgcttca gctcgcccga ctggaacatc tcgcgcacga
     1801 tgtcggcgcc gccgatgaat tcgcccttga cgtaaagctg ggggatggtg ggccagtcgg
     1861 aatagagctt gattccctcg cgcaaggatt cgtcctgcaa cacgtcgacg ccgacatagt
     1921 cgacgccgag gtggtccagc acctgcacca cgaggccgga gaagccgcag cgcggctgtt
     1981 cggggacgcc cttcatgaac agcaccacgt cgtggccctt gatggtgtcg tcgatgaagg
     2041 tgttgacgtc gtcggtcacg cggaactctc ctgcaagcta tgtccacgat ataggcgcgg
     2101 gcggcgcgaa aatccagagg ccaaaatcca gaggccagaa tccagggatc agggcgcgat
     2161 cagcgccgcg cggcgccgat ctggtcgttg aagcgcgcca tctcgatctg ggcgggcacg
     2221 gaggagggca gggcgcgctc ggacacggcg cacagcgcct cgatctgctg cggcgtctcg
     2281 gcgtcgatga acacgcagct caggctgggc tgggccagcg catagccgag gcacagctcc
     2341 tgcggcgtcc agtcgggcgt ggtgtgcaaa aaggcgtgcg tgcccgcccc ggccagcgga
     2401 ttttccggcc tggcgccgaa ccagccgcgc cgcgcggcct tcggcacgac gtcggacgcc
     2461 ttgcggtagg cgtcgggaaa gaagtcgccg ccgatgacgg tgatctcgct ctgcacggcg
     2521 cggtcgatct gatggcgctt gtcccaggtc gaatcgatgt cgaagctggt ttcgatgacg
     2581 ttgaaatggc cgtccgcgat cagcagcgcc atgtcgcccg cgtcgccgcg cgcgccgatg
     2641 tagcgcaaca tgcgcgcgcg cttgaggctg tcgaggaagc tcagcgtgtc ggcgggcagt
     2701 tgcagcgcgc ccggcggcga gaacatcaga aggtccagcg cctgcaaacc ggtgtccttg
     2761 atcgccgcgc gcaggcgctc cttcagcggg cgcagcgcat agcccgtgct gtcgcaggct
     2821 tgccccgcct cctgcgcgtc ggcgctgacg aacagaaggc ggcgctcgac gaccgaaaag
     2881 gcctgcgccg ccgcgcgcag gaagtccggc gcggtggtgt cgaaatggta ggtgttgacg
     2941 ccgttctcca gcccggccat gatcagcttg ttcagcagcc cgacattacc gccgattttc
     3001 aggcgcgaca ggcgcaggcc gatggtcgac agcgccagac cggaacgtcc gaaggggcgg
     3061 tagcgcatgg ctcagcgccc ccggtccggg ccggtcgcgc aggccgccgt tttcgctgat
     3121 cccttcgtgg tcatggccgc tttgcagaag aaatggtctg ggtagaagaa ctgggcagaa
     3181 gaaatagtct gggaaagggt cggccgccgt ctcgcccctg acgcgacgga accggtccaa
     3241 gtcttggcgg caaaccggtt aacggagttt taaggctgac ggatttggcg cgcgccgcgt
     3301 gcgggcgtat gctgccgcga acgggaggga aaagatgagg cggttcggtt ttctgctggc
     3361 cttggcggtg atggcgcaac cggcgcaggc gtgtcccgat ccggccgccg cgctcgactc
     3421 cgcgctggcc gcgacgatcg ccgcggtgcg cggcggcgac gcgcaggccc tgttaggccg
     3481 gatgagcgcc gaaggcgtca gcttcggccc cgacgcgccg ccgacgccgc tggccgtgtt
     3541 gcgggcgcag ttcgcggatc gcggcgggcg gtattgcgat gtgttcgcct gcaacggcgt
     3601 cgagggggcg ctgcatcggc ggttc
//
LOCUS       DASVLL010000054         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_217593,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000054 DASVLL010000000
VERSION     DASVLL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217593"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF402_00940"
     CDS             complement(<1..291)
                     /locus_tag="VF402_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798882.1"
                     /translation="MPLRIQRQAHIHTLMRDNSLLGLLEDAACARLAADMEVMHLDRG
                     DILVDVGETVKYAYFPCNGTMVSYLVVLPNGSTVETGLIGREGAVSGVVSHGS"
     gene            494..1258
                     /locus_tag="VF402_00945"
     CDS             494..1258
                     /locus_tag="VF402_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7798883.1"
                     /translation="MTTAESELTEALRRAETAERASRAKSDFLAVMSHEMRTPLSSIA
                     GAAYLLTRAPELSPQSRRLVETLRAGADALLTLLNDFLDISKIEAGRLALEPAPFRLS
                     QVLAQVETIMAARALGAALRLEVDCVEPPGRLPVGDARRLQQILLNLAVNAVKFTSQG
                     VVRITGVLTETDSRSGALTLAVADTGVGIAPERLADVFEPFTQVHGGDAHEGTGLGLS
                     IVRHLVAAMHGQTHVESAPGVGTTFTVVIPLPLTPA"
     gene            complement(1337..>1895)
                     /locus_tag="VF402_00950"
     CDS             complement(1337..>1895)
                     /locus_tag="VF402_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX7798884.1"
                     /translation="DEYLHEAGAAAPKVQDGDMAAIASPLDFVALNIYTPEWVRADDG
                     PKGYAVIPHNAESPRMASPWLVVGPEVAYWGPRMVSELWGPKTLYISENGASADDAIV
                     NGHIDDADRVMYLRNYFENVRRLVVEDYPLKGYFLWSLMDNFEWADGYTKRFGIHYVD
                     FATQKRTPKLSALWYKEVIRQSRIV"
BASE COUNT          328 a          605 c          622 g          340 t
ORIGIN      
        1 gctgccgtgc gagacgacgc cgctgaccgc gccttcgcgc ccgatcaggc cggtttcgac
       61 cgtcgagccg ttaggcagga ccaccagata ggacaccatg gtcccgttgc atgggaaata
      121 agcgtatttc accgtctcgc cgacatcgac cagaatatcg ccgcggtcga gatgcatgac
      181 ctccatgtcg gccgccagtc tcgcgcacgc cgcgtcctcc agaagaccca gcaggctgtt
      241 gtcgcgcatc agggtgtgaa tgtgcgcctg tctttgtatc cgcaatggca tgaaactccc
      301 tgcccgcgtg tgtgtactat tggacttaca ggctgaagaa atctgaatat gattacgatg
      361 agcctgcggc ggaagagcgc tttcttttcc ataaaatttc gcctttccag ccagttggcc
      421 gttgtgaaac ggcatagaag gagacggagc gctgctccgc gccatgcatg acgccatgtt
      481 caacgacgca cacatgacca cagcggagag cgaactgacc gaggcgctcc gccgcgccga
      541 aaccgccgag cgcgccagcc gcgccaagtc ggactttctg gccgtgatga gccatgagat
      601 gcgcacgccg ctcagctcga tcgcgggcgc ggcctatctt ttgacgcgcg cgccggagtt
      661 gtcgccgcaa agccgcaggc tggtcgagac cttgcgggcc ggggcggacg cgctgctgac
      721 cctgctgaac gattttctcg acatctcgaa gatcgaggcc gggcggctgg cgctggagcc
      781 ggcgccgttc cggctgtcgc aggtgctggc tcaggtcgag acgatcatgg cggcgcgcgc
      841 cctcggcgcg gccttgcgcc tcgaggtcga ttgcgtcgag ccgcccggac gcctgccggt
      901 cggcgatgcg cgccggttgc agcagatcct gctgaacctc gccgtcaatg cggtcaagtt
      961 cacctcgcaa ggcgtggtgc gtattacagg cgttcttacg gaaacggaca gccggtcagg
     1021 cgccctgact ctggcggtcg ccgacaccgg cgtcggcatc gcgccggagc gtctggcgga
     1081 tgtgttcgag ccgttcacgc aggtgcatgg cggcgacgcg cacgaaggca cgggcttggg
     1141 cctgagcatc gtgcggcatc tggtcgcggc gatgcatggc caaacccatg tcgagagcgc
     1201 gccgggcgtc ggcacgacct tcacggtcgt catccccctg ccgctgacgc ccgcctgagc
     1261 ggtcgcggac aacgcgccga aagcggaaac agttgaggga tgcaaggccg accggccccg
     1321 catccctcgt aacagcctac acgatccggc tctggcggat gacttccttg taccagagcg
     1381 cgctgagctt gggcgtgcgc ttctgggtgg cgaaatcgac gtaatggatg ccgaagcgct
     1441 tggtgtagcc gtcggcccac tcgaaattgt ccatcaggct ccagaggaaa tagcccttga
     1501 gcggatagtc ctcgacgacg aggcggcgga cgttttcgaa atagttgcgc aggtacatga
     1561 cccggtcggc gtcgtcgata tggccgttga cgatggcgtc gtccgccgag gcgccgtttt
     1621 cggagatgta gagcgtcttt ggcccccaca gctcgctgac catgcgcggc ccccaatagg
     1681 cgacctccgg cccgaccacc aaccacggcg aggccatgcg cggcgattcg gcgttgtgcg
     1741 gaatgaccgc atagcccttg gggccgtcgt cggcgcgcac ccattccggc gtatagatgt
     1801 tgagcgcgac gaaatcgagc ggactggcga tggcggccat gtcgccgtcc tgcaccttcg
     1861 gcgcggcggc gccggcttcg tgcagatatt cgtcg
//
LOCUS       DASVLL010000055         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_325212,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000055 DASVLL010000000
VERSION     DASVLL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325212"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VF402_00955"
     CDS             <1..252
                     /locus_tag="VF402_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX7798885.1"
                     /translation="YRSANVAKNFVSSLNAMVASGIFIAQGAVAWPQTLSLMAGMICG
                     GLAGAYVARVIPAKVARGMIVVVGAALTIVFAKLYWFDA"
     gene            complement(257..1102)
                     /locus_tag="VF402_00960"
     CDS             complement(257..1102)
                     /locus_tag="VF402_00960"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7798886.1"
                     /translation="MSVDYEVEYNNRARVPDHPEIFVRWEHEGADYRKASPKAALGLK
                     YGPSERQTLDIFPAANDGPDTPLALFIHGGWWRSLHPSSFSQMAKGPNAHGVTVAVAG
                     YDLCPQVSIATIVEQMRAAGLFLWRKYKKRFVVYGHSAGGHLTACLVATDWKTAAPDA
                     PHDLAPAGYAISGVFDLEPLTKISVNQDLKLTDKSAHDLSPLYWQVPPGRILDAVVGA
                     QESNEFLRQSKTIAEAWRQGMATTRYEAPPGNHFTIVDPLADPASAMTRRVVELARQV
                     QAGNR"
     gene            1192..>1629
                     /locus_tag="VF402_00965"
     CDS             1192..>1629
                     /locus_tag="VF402_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798887.1"
                     /translation="MKVDGKPMRTIWVEDDGAACGIIDQTRLPHRFETLRLSTVEDAA
                     HAIRSMQVRGAPLIGATAAYAVWLAMRADASDEALERAYALLLATRPTAINLKWALDE
                     MVATLRNRPRAERAAAALKRAREIADEDVDINQSIGRHGLKLIE"
BASE COUNT          262 a          551 c          545 g          271 t
ORIGIN      
        1 tatcgctcgg ccaacgtggc gaagaacttc gtctccagcc tcaacgccat ggtcgcctcc
       61 ggcatcttca tcgcgcaggg cgcggtcgcc tggccgcaga ccttgtcgct gatggccggc
      121 atgatctgcg gcggccttgc cggcgcctac gtcgcgcgcg tcatcccggc gaaggtcgcg
      181 cgcggcatga tcgtggtggt cggcgcggcg ctgaccatcg tctttgccaa attgtactgg
      241 ttcgatgcgt gacggttcag cggttcccgg cctgcacctg ccgcgccagt tcgaccacgc
      301 gcctggtcat cgcgctggcc ggatcggcca gcggatcgac gatggtgaag tggttgcccg
      361 gcggcgcttc gtaacgggtc gtcgccatgc cctgccgcca cgcttcggca atcgtcttgc
      421 tctggcgcag gaactcgttc gactcctgcg cgccgaccac cgcatcgaga atgcggccgg
      481 gcggcacctg ccaatagagc ggcgacagat cgtgcgcgct tttatccgtg agcttcaggt
      541 cctggttgac cgagatcttg gtgagcggct ccagatcgaa cacgccggag atcgcatagc
      601 ccgcaggcgc gaggtcgtgc ggcgcgtccg gcgccgcggt cttccagtcg gtcgcgacca
      661 ggcacgcggt cagatggccg ccggcggaat ggccatagac cacgaagcgc ttcttgtatt
      721 tccgccacag gaacagaccc gccgcgcgca tctgctcgac gattgtcgcg atcgacacct
      781 gcggacacag gtcgtagccg gcgacggcga cagtgacgcc gtgcgcgttc gggcccttgg
      841 ccatctggct gaagctcgag ggatggagcg aacgccacca gccgccatgg atgaacagcg
      901 ccagcggcgt gtccggcccg tcattggcgg ccgggaagat gtcgagcgtc tgccgttccg
      961 acggcccgta tttgagaccg agcgcggcct tcggcgaggc cttgcggtag tccgcgccct
     1021 cgtgctccca gcggacgaaa atctccggat ggtccggcac gcgggcgcgg ttgttatatt
     1081 cgacctcgta atcgacgctc atatgtctct ccgcaatttc gcccgcagag tgcggcaaag
     1141 cccgggctgg ggcaaggcga caggcgcgtc aaaaacggct agatcagcga catgaaagtc
     1201 gacggcaaac cgatgcggac gatctgggtg gaggacgatg gcgccgcctg cggcatcatc
     1261 gatcagaccc gcctgccgca tcgcttcgag acgctgcgcc tgtcgaccgt cgaggacgcc
     1321 gcccacgcca tccggtcgat gcaggtccgc ggcgcgccgc tgatcggcgc caccgccgcc
     1381 tacgccgtct ggctcgccat gcgcgctgac gcctccgacg aggcgctgga gcgcgcctat
     1441 gcgttgctgc tggccacgcg cccaacggcg atcaacctga aatgggcgct cgatgaaatg
     1501 gtggcgaccc tgcgcaaccg gccgagggcc gagcgcgccg cggccgcgct caagcgcgcg
     1561 cgggagatcg ccgacgagga cgtcgacatc aatcagtcga tcggccgcca cggtctcaaa
     1621 ttgatcgag
//
LOCUS       DASVLL010000056         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_164549,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000056 DASVLL010000000
VERSION     DASVLL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164549"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VF402_00970"
     CDS             complement(<1..354)
                     /locus_tag="VF402_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7798888.1"
                     /translation="MTRLFLPGVGGDPAFWRPVADRLPPRADDVFFGWPGLGDQPADP
                     KTRGYDDLIALVAARLDRPADLIAQSMGGALAIRLALAHPGKVRRLVLTATSGGMAMR
                     GFGASDWRDEYRRLFP"
     gene            complement(351..1013)
                     /locus_tag="VF402_00975"
     CDS             complement(351..1013)
                     /locus_tag="VF402_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7798889.1"
                     /translation="MPEVILAGAAGRIEARYSQGKSENAPIALILHPHPKAGGHMNNP
                     VTVQLFHLFMTRGFSVLRFNFRGVQKSQGEFDAGIGELADAATALDWLQAKNPTASQF
                     WVAGYDFGAYIGMQLLMRRPETDGFISVSPPTNAYDFSFLAPCPASGMFLHGGSDSIV
                     PTAEVDRVVAKLRTQKGIVIDAEVVPEANHFWADHLSEVESRVGAYIDRRLAERTADS
                     AE"
     gene            1204..1719
                     /locus_tag="VF402_00980"
     CDS             1204..1719
                     /locus_tag="VF402_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7798890.1"
                     /translation="MRLSTKGRYAVMAMTDLALNGQFGANGARPISLSDIAERQQISL
                     SYLEQLFARLRKAGLVRSARGPGGGYALARPAAEMFVSEIVLAVDEPIKATRCSMTSN
                     KLGANKRLSQDEARREIGCMPGGQRCLTHNLWEDLGVAIHDYLSQVSLEDVIRRRTRK
                     TAPSRMEAVVS"
     gene            1716..>2583
                     /locus_tag="VF402_00985"
     CDS             1716..>2583
                     /locus_tag="VF402_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7798891.1"
                     /translation="MSPLYLDHAATSPLRPEARAAFLRASDMGGNASSIHALGRKAKL
                     LLEEARADLLAGLNATGAAGLVFTSGGTEANAMALRQAQGCDYIVLSAGEHDSLYGAA
                     KDAAIAPLTKDGVIDLAALAALLDRPGRPFVGVMLANNETGAINDVAAVAGIVHAHGG
                     WLHVDAVQALGKIAIDFRALGAESLSLSAHKIGGGQGVGAFVHDRDRTPAALLRGSGQ
                     ELGLRAGTENIPGVAAFAAAFKACQPMTESVLSAQASVERSLKALGVTILAEGAGRVP
                     GLTAFAQPHWPSP"
BASE COUNT          419 a          829 c          919 g          416 t
ORIGIN      
        1 cggaaacagg cgccggtact catcgcgcca gtcggacgcg ccgaagccgc gcatggccat
       61 gccgccggac gtggcggtca gcacaagacg gcggaccttg ccgggatggg ccagagccag
      121 acgaatcgcc agcgcgccgc ccatcgactg cgcgatcagg tcggcgggac ggtcgaggcg
      181 cgcggcgacc agagcgatca ggtcgtcata gccgcgcgtc ttcggatcgg cgggttggtc
      241 gcccaagccc ggccagccga aaaacacatc gtccgcgcgc ggcggcagcc ggtcggccac
      301 gggccgccag aaggccggat cgccgccgac gccgggcagg aacagccgcg tcattcggcg
      361 ctgtcggccg ttctttcggc cagccgccgg tcgatatagg cgccgacgcg gctttcgacc
      421 tcgctgagat ggtcggccca gaagtgattg gcctccggca cgacctcggc gtcgatgacg
      481 atgcccttct gcgtgcgcag cttggccacc acgcggtcga cctcggcggt cggcacgatc
      541 gaatcggagc cgccgtgcag gaacatgccg gacgccgggc agggcgccag aaagctgaaa
      601 tcataggcgt tggtcggcgg cgacaccgag atgaagccgt cggtttccgg gcggcgcatc
      661 aggagttgca tgccgatata ggcgccgaaa tcgtaaccgg cgacccagaa ttgcgaggcg
      721 gtcgggttct tggcctgcag ccagtcgagc gccgtcgccg cgtcggccag ttcgccgatc
      781 ccggcgtcga actcgccctg gcttttctgc acgccgcgga agttgaagcg caaaacggag
      841 aagccgcgcg tcatgaacag gtggaacagt tgcaccgtca ccgggttgtt catatggccg
      901 cccgccttgg gatgcggatg caggatcaag gcgatgggcg cgttttccga cttgccctgg
      961 ctgtaacggg cttcgatgcg accagcggct ccggccagga tgacttcggg cattcaatca
     1021 ccttgaggta gttctgcgaa acaggttgtg tgcgccgcaa tacttgactg ctttagtcgg
     1081 aaattgctaa acttccgtcc atagccggcg cgggcagatg cgcctcttag cacaaagact
     1141 tctctggaca caaagaattc tcttgcccgc ccgccgcgtt tcaaccacgg caaggatttg
     1201 tccatgcgcc tgtcgaccaa gggacgttac gccgtcatgg ccatgacgga cctggcgctg
     1261 aacggccagt tcggcgccaa cggcgcgcgt ccgatttcgc tgtccgacat cgccgagcgc
     1321 cagcagattt cgctgtccta tctcgaacaa ttgttcgcgc ggctgcgcaa ggccgggctg
     1381 gtgcgcagcg cgcgcggccc cggcggcggc tatgcgctgg cgcgtccggc ggcggagatg
     1441 ttcgtgtccg agatcgtgct ggcggtcgac gagccgatca aggcgacgcg ctgctcgatg
     1501 acctcgaaca agctgggcgc caacaagcgg ctgagccagg atgaggcgcg gcgcgagatc
     1561 ggctgcatgc cgggcggcca gcgctgcctg acgcacaatc tctgggaaga cctcggcgtc
     1621 gccattcacg actacctgtc gcaggtgtcg ctggaagacg tgatcaggcg gcgcacgcgc
     1681 aagacggcgc cgtcgcggat ggaggccgtc gtttcatgag cccgctctat ctcgatcacg
     1741 ccgcgacgtc gcccctgcgc cctgaggcgc gcgcggcgtt tctgcgcgcg tccgacatgg
     1801 gcggaaatgc gtcgtcgatc catgccttgg ggcggaaagc caagcttttg cttgaggagg
     1861 cgcgcgccga tctgctggcc gggctgaacg ccacgggcgc ggcggggctg gtctttacct
     1921 ccggcgggac ggaggccaat gcgatggcgc tgcgtcaggc gcaagggtgc gattatatcg
     1981 tgctgtcggc gggcgagcat gacagccttt acggcgcggc gaaggacgcg gcgatcgcgc
     2041 cgctgacgaa ggacggcgtg atcgacctgg cggcgctggc ggcgttgctg gacaggccgg
     2101 gcaggccgtt cgtcggcgtg atgctggcca ataacgagac gggcgcgatc aacgacgtcg
     2161 ccgccgtggc cgggatcgtg catgcgcatg ggggctggct gcatgtcgac gcggtgcagg
     2221 ccctgggcaa gatcgctatc gatttcaggg cgctgggcgc ggagtcgctg tcgctgagcg
     2281 cgcacaagat cggcggcggg cagggcgtcg gcgccttcgt ccacgaccgc gaccgcacgc
     2341 ctgcggccct gctgcggggc agcggtcagg aactggggct gcgcgcgggc accgagaata
     2401 ttccgggcgt cgccgccttc gccgccgcct tcaaggcttg ccagccgatg acggagtccg
     2461 tattgagcgc gcaagcgtcc gtcgagcgct cgctaaaggc gctgggcgtc accatattgg
     2521 ccgaaggggc gggccgcgtc cccggcctta cggccttcgc gcagccgcat tggccgtcgc
     2581 cgc
//
LOCUS       DASVLL010000057         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_110532,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000057 DASVLL010000000
VERSION     DASVLL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110532"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF402_00990"
     CDS             complement(<1..555)
                     /locus_tag="VF402_00990"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX7798892.1"
                     /translation="MSRIRLGLNVDHVATVRNARGGRAPDPERAARLALQAGVDGITA
                     HLREDRRHILDRDIAALKAVCDAYHKPLNFEMAVTDEMIGIALDLKPHAACLVPERRQ
                     ERTTEGGLDVAGRIETVGAAVAKFNAAGIRASLFIAADPEQVAAAARIKAPVVEFHTG
                     ALCDAWRDQEDYLFESELNRLQAAA"
     gene            complement(559..1146)
                     /gene="pyrE"
                     /locus_tag="VF402_00995"
     CDS             complement(559..1146)
                     /gene="pyrE"
                     /locus_tag="VF402_00995"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7798893.1"
                     /translation="MTSEDVINEFRAANALREGHFVLSSGLHSPMFLQKNLVFMNGER
                     CAKLCKALAEKITARIGQVDVAISPAVGGIIPGYETARHLGVDSMYVEREGGEFRLRR
                     GFHIEPGAKVVMVEDIVTTGLSSRECIEAIRKAGGNVVAAACIVDRSGGKADVGAPLI
                     ALASLSVPAYPADNLPPELAAIPVEDPGSRRLAKA"
     gene            complement(1347..1837)
                     /gene="eno"
                     /locus_tag="VF402_01000"
                     /pseudo
     CDS             complement(1347..1837)
                     /gene="eno"
                     /locus_tag="VF402_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080195.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
BASE COUNT          302 a          603 c          597 g          335 t
ORIGIN      
        1 agccgccgcc tgcaagcgat tgagttcgct ctcgaaaaga tagtcttcct gatcgcgcca
       61 cgcatcgcag agcgcgccgg tgtggaattc gactaccggc gctttgatcc tggcggcggc
      121 ggcgacctgc tcagggtcgg cggcgatgaa cagcgacgcg cggatgcccg cggcgttgaa
      181 ttttgcgacg gcggcgccga cggtctcgat ccgtccggcg acgtccagcc cgccctcggt
      241 cgtgcgctcc tgacggcgct ccggcaccag gcaggcggcg tgcggcttga gatcgagcgc
      301 gatgccgatc atttcgtcgg tcacggccat ctcgaaattg agcggcttgt gatacgcgtc
      361 gcacaccgct ttcagcgccg cgatgtcgcg gtcgaggata tggcggcggt cttcgcgcag
      421 atgcgccgtg atcccgtcca cgcccgcctg cagggccagc cgcgccgcgc gttcggggtc
      481 gggcgcacgg ccgccgcgcg cgttgcgcac ggtggcgacg tgatcgacgt tcaatcctaa
      541 gcggatgcgc gacatggctc aggctttcgc gagacggcgg ctgccgggat cctcgaccgg
      601 gatggcggcc agttccggcg gcaggttgtc ggcgggatag gcggggacgc tgagcgaggc
      661 cagcgcgatc agcggcgcgc cgacatcggc cttgccgccg gagcggtcga cgatgcaggc
      721 cgccgcgacg acgtttccgc ccgccttgcg gatcgcttcg atgcattcgc gcgacgacag
      781 gccggtggtg acgatgtcct cgaccatgac gaccttcgcg cccggctcga tatggaagcc
      841 gcggcgcagg cggaattcgc cgccttcgcg ttcgacatac atcgaatcga cgccgaggtg
      901 tctggcggtt tcgtagccgg ggatgatgcc gccgaccgcc ggggagatgg cgacgtcgac
      961 ctgtccgatc cgcgccgtga tcttttcggc cagcgccttg caaagcttcg cgcagcgttc
     1021 gccgttcatg aagacgaggt tcttttgcag gaacatcggc gaatgcagac cggaggacag
     1081 gacgaaatgg ccttcgcgaa gggcgttggc ggcgcggaat tcgttgatca cgtcttcgga
     1141 ggtcatggtc ggtcctttgc agctttcggg ggcggtttat cgcgttcggc cggtcgtcgt
     1201 ccactgctaa cttatggggc gtgggggcga ttgcccccac atcttctttt tcaataaaaa
     1261 agccccgccc taagacgagg cttttttatt gaaggacaag agctggggcc aattggcccc
     1321 agaccccgta agttagcagc gcccaatcaa caccgaatga cgccgacgcc cgcatacacc
     1381 gcctcgtcgt ccagcagcgc ctcgatgcgc agcaactggt tgtacttggc gatccggtcc
     1441 gaacgcgcca gcgacccggt cttgatctgg ccgcagttca tcgccaccgc caggtccgcg
     1501 atggtcgaat cctccgtctc gcccgaacgg tgcgacatca ccgacgtata gcccgcccgg
     1561 tgcgccatat cgaccgcgtc catcgtctcc gacaacgtgc cgatctggtt gaccttcacc
     1621 agcagcgaat tggccatgcc ctcgacaatg cccgtttcca gacggtccgg gttggtcacg
     1681 aacaggtcgt cgccgacgat ctgcacgcgg tcgcccagac gatcggtcag cagacgccag
     1741 ccctcctaat cgtcctcggc catgccgtcc tcgatcgagc agatcgggaa cttgtcggcc
     1801 agaccggcca gataatcgac catctgagcg gcgtcca
//
LOCUS       DASVLL010000058         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_486667,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000058 DASVLL010000000
VERSION     DASVLL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486667"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="VF402_01005"
     CDS             complement(<1..1550)
                     /locus_tag="VF402_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7798894.1"
                     /translation="MPYLRHALSLALLLTSASALPALAATPAACADKPCAYLDTSLTP
                     EARAKDLVSRMTLAEKAAQMQDNAPAIPRLGLKRYGWWNEALHGVARAGHTTVYPQAI
                     ALAATWDPGLMNDIGKAIADEGRASHNAALARDPSGTDRYYGVNYWSPNINIFRDPRW
                     GRGQETYGEDPYLTGRMAVGFITGIQQQANGVYEGVATPKHFAVHSGPEPLRHGFNVP
                     VSPFDLEDTYLPAFRAAFTEGHAYSTMCAYNATDSFPMCASPLLAQRIRGDWGFKGFI
                     VSDCDAVDDMVTGHKSEPDAAHAAAAAVKAGTDLDCGTTYDALPQAVKDGLISEAEVD
                     TALERLMVARIRMGLIDGSPYDAIPAGDLNAPEHRRLALRAAQEAIVLLKNGGALPLA
                     GVKKIAVIGPNADLLQSVEGNYTGSPLDPSLPLPALRDAFGAANVLYAAGAPLTEGMR
                     MPVPETYLKPAADSAEHGLSGQYFDNTDFSGAPAFTRTDRVINFDWYHTAPKGLKPLG
                     FSVRWTGV"
     gene            complement(1626..2213)
                     /locus_tag="VF402_01010"
     CDS             complement(1626..2213)
                     /locus_tag="VF402_01010"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX7798895.1"
                     /translation="MADDMTGVVVLAGGRGARMGGDKPFHAWGATTLIEAVLTRLEPQ
                     AADIAVNAGALDAPHATRLAGLGLPVLYDDLPWRGPLSGVLSALQWARLGGDTYVITC
                     PCDMPNLPDDYVARLTNAPDAAVAHYGGADSYPLCARWRADLAEALETALRAAPGGLG
                     VMRFASAFTPLIVPVSDPAAFANINQINPAQRPDA"
     gene            complement(2291..>2474)
                     /locus_tag="VF402_01015"
     CDS             complement(2291..>2474)
                     /locus_tag="VF402_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798896.1"
                     /translation="KLLTHETLAFDPLGDFRAVDYALPPDAAQGGNIHVRFVTKNSDL
                     ALFEARTLRAAAAKTA"
BASE COUNT          371 a          836 c          868 g          399 t
ORIGIN      
        1 ataacgcctg tccaacgcac ggaaaagccg agcggtttca gccccttggg cgcggtgtgg
       61 taccaatcga aattgatcac ccggtcagtg cgcgtaaagg cgggcgcgcc cgaaaaatcc
      121 gtattgtcga aatactggcc gctcagcccg tgttcggcgc tgtccgccgc cggtttcaaa
      181 taggtctccg gcaccggcat ccgcatccct tcggtcagcg gcgcgcccgc ggcgtacagc
      241 acattggccg cgccgaaagc gtcgcgcagc gcgggcaggg gcagggacgg atcgagcggc
      301 gagccggtgt aattgccctc gacgctctgc aacaggtcgg cgttcggccc gatcaccgcg
      361 atctttttca cgcccgccag cggcagggcg ccgccgttct tcagcagcac gatcgcctct
      421 tgcgccgcgc gcagcgccag gcgccggtgt tcgggcgcgt tgagatcgcc cgccgggatc
      481 gcgtcatacg ggctgccgtc gatcagcccc atgcggatgc gggcaaccat cagccgctcc
      541 agcgccgtat cgacctcggc ctcgctgatc aggccgtcct tcaccgcctg cggcagggcg
      601 tcgtaggtgg tcccgcaatc gaggtcggtc cccgccttga ccgccgccgc cgcggcatgg
      661 gcggcgtccg gctcggactt atggccggtg accatgtcgt cgaccgcgtc gcagtcggag
      721 acgataaagc ccttgaagcc ccagtcgccc cggatgcgtt gcgccagcag cgggctggcg
      781 cacatcggga agctgtcggt ggcgttatag gcgcacatgg tcgaataggc gtggccctcg
      841 gtgaaggcgg cgcggaaggc gggcaggtag gtgtcttcga ggtcgaacgg cgacaccggc
      901 acgttgaagc cgtggcgcag gggttccggc ccggaatgga cggcgaaatg cttgggcgtg
      961 gccacgcctt catagacgcc gttcgcctgc tgctggatgc cggtgatgaa gccgaccgcc
     1021 atccggccgg tcagatacgg atcctcgcca taggtttcct gcccgcgccc ccagcgcgga
     1081 tcgcggaaga tgttgatatt gggcgaccag tagttgacgc cgtaatagcg gtccgtgccg
     1141 gacggatcgc gcgccagggc ggcgttgtgg ctggcgcggc cctcgtcggc aatcgccttg
     1201 ccgatgtcgt tcatcaggcc gggatcccac gtcgcggcca gggcgatggc ctgcggatag
     1261 accgtggtgt gcccggcgcg cgccacgccg tgcagcgcct cgttccacca gccgtagcgt
     1321 ttcaggccga ggcgcggaat ggcgggcgcg ttgtcctgca tctgcgccgc cttttccgcc
     1381 agcgtcatgc gcgagacgag gtctttggcg cgcgcctccg gcgtcagcga cgtgtcgaga
     1441 taggcgcacg gcttatcggc gcaggcggcc ggggtcgccg ccagagcggg cagggcggac
     1501 gcgctggtca gcagcagggc cagcgaaaga gcatgacgca gatacggcat cgggggcctc
     1561 ccggaatgtt tcctcgccct tttgcgggct tatctggtac cgctatcatc gcgggtcgcg
     1621 cagcgtcaag cgtccgggcg ttgcgccggg ttgatttggt tgatattggc gaaggcggcg
     1681 ggatcgctta ccggcacgat cagcggcgtg aaagcgcttg caaaacgcat gacgccaagg
     1741 ccgcccggcg ccgcccgcaa cgcggtttcc agcgcctcag ccagatcggc gcgccagcgg
     1801 gcgcacaggg gatagctgtc cgcgccgccg taatgagcga cggcggcgtc cggcgcgttc
     1861 gtcaggcgcg cgacatagtc gtccggcagg ttcggcatgt cgcacgggca ggttatgaca
     1921 taggtatcac cgccgagccg cgcccattgc agcgcgctca gcaccccgct cagcgggccg
     1981 cgccacggca ggtcgtcgta cagcaccggc agccccaggc ccgccagccg cgtggcgtgc
     2041 ggcgcgtcca gcgcgccggc gttgacggcg atgtccgcgg cctgcggctc cagccgcgtc
     2101 agcacggcct cgatcagggt ggtcgcgccc catgcgtgga acggcttgtc cccgcccatg
     2161 cgcgcgccgc gtccgcccgc cagtacgacc acgcctgtca tatcgtccgc cacatggatc
     2221 gcctccgcaa accgccccga ttatgtgcgg cgcagcgatt cccggcaagg ggctcccggc
     2281 aaggggcgtc tcaggccgtc ttcgcggcgg cggcacgcag ggtgcgcgcc tcgaacaggg
     2341 ccagatcgct gttcttcgtc acgaagcgca catggatgtt tccgccttgc gccgcgtccg
     2401 gcggcagggc gtaatcgacg gcgcggaaat cgccgagcgg atcgaaggcc agggtttcgt
     2461 gcgtcagcaa tttg
//
LOCUS       DASVLL010000059         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_110574,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000059 DASVLL010000000
VERSION     DASVLL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110574"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VF402_01020"
     CDS             <1..983
                     /locus_tag="VF402_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7798897.1"
                     /translation="DAALSAMAKVGLTGVGDPGIDLATYRLYRQYADRHKLTARIYAM
                     NRNTGADFDTISRSGPLVGYGGDFLTVRAVKLFADGALGSRGAALLSPYSDDSHNRGL
                     LFQPQPELDTMIEKAFGKGYQVCIHAIGDRANREVLDGFAAAYKVHPEAKAFRNRVEH
                     AQIVAPEDIPRFKTLGLIASMQPTHATSDMNMAEDRIGHDRMAGAYAWRTFLDQGTVI
                     AGGSDFPVESPNPFFGLYAAVTRQDHQGQPPGGWYPAQKMTLVEALRAFTLDAAYAEH
                     AEATQGTLEPGKWADFILVDRDIVAGPPADIWKTRVLQTWVGGKQVYAAE"
     gene            1227..1550
                     /locus_tag="VF402_01025"
     CDS             1227..1550
                     /locus_tag="VF402_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798898.1"
                     /translation="MKTPNLLALSAAVLITAAGLASMGHSVTRIPVAEIDGIRVIDLA
                     PVRVSPTAEDLRNAALTAVTTTVGTPAVNGLGGHADGANPLVGAQMAMPYYSFGNKIG
                     RIGKD"
     gene            1555..1929
                     /locus_tag="VF402_01030"
     CDS             1555..1929
                     /locus_tag="VF402_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7798899.1"
                     /translation="MTITWNDSVPIYRQLRDRVVAMILDGALNEGDPLPSVRQVAADF
                     QINPLTVSKAYQELVDDQLVEKRRGLGMFVNDGAREALLKSERERFLREEWPALFARL
                     QRLGLDLKTLLREAADGPEKTA"
     gene            1926..>2223
                     /locus_tag="VF402_01035"
     CDS             1926..>2223
                     /locus_tag="VF402_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7798900.1"
                     /translation="MNAVITARGLRKQYKNTLALDDVSFSIPSGRIVGLIGPNGAGKT
                     TALKAILGLTPFEGELHVLGLDPRTQRNKLMEQVCFIADVAVLPRWMRVRDAIDF"
BASE COUNT          368 a          814 c          715 g          326 t
ORIGIN      
        1 tggacgccgc gctgtccgcc atggccaagg tcggccttac cggcgtcggc gacccgggca
       61 tcgacctggc cacctaccgc ctctaccgcc agtacgccga ccggcacaag ctcaccgcgc
      121 gcatctacgc gatgaaccgc aacaccggcg ccgacttcga caccatcagc cgcagcggcc
      181 cgctggtcgg ctacggcggc gatttcctca ccgtgcgtgc ggtcaagctg ttcgccgacg
      241 gcgcgctggg cagccgtggc gcggcgctgc tgtcgcccta ctccgacgac tcgcacaacc
      301 gcggcctgct gttccagccg caacccgaac tcgacacgat gatcgagaaa gcgttcggca
      361 agggctacca ggtctgcatc cacgccatcg gcgaccgcgc caaccgcgaa gtgctggacg
      421 gtttcgccgc cgcgtacaag gtgcatcccg aggcgaaggc gttccgcaac cgcgtcgagc
      481 atgcgcagat cgtcgcgccg gaggacatcc cgcgcttcaa gacgctcggc ctgatcgcct
      541 cgatgcagcc cacccacgcc acctcggaca tgaacatggc cgaggaccgc atcggccacg
      601 accgcatggc cggcgcctac gcctggcgca ccttcctcga ccagggcacg gtgatcgccg
      661 ggggctcgga cttcccggtg gagtcgccca acccgttctt cggcctctat gcggccgtga
      721 cccggcagga ccaccagggc cagccgcccg gcggctggta cccggcgcag aagatgaccc
      781 tggtcgaggc cctgcgcgcg ttcaccctgg acgccgccta cgccgagcac gccgaagcca
      841 cccagggcac gctggagccc ggcaagtggg ccgacttcat cctggtcgac cgcgacatcg
      901 tcgccggtcc gcccgccgac atctggaaga cccgcgtgct gcagacctgg gtcggcggca
      961 agcaggtgta cgccgcggag taggcgtcgg gcgggcccgc ccgacgcgcg gcggcgcccg
     1021 gaatccgcgt gctggcgggg ggaacgcggt cgtatggtgt gcgctcctgg ccgtgcgcac
     1081 acccaggcgt gcgaccgcct cggcccgctt cccggcatag gccatcagtc atgttgcgat
     1141 cttcggcttc ggctattgac ggtttcggaa gtggtgttat agttcactac aacaccggtc
     1201 cacgaggtca ctaaaggaga cctgccatga aaacgccgaa cctgctcgcc ctttccgccg
     1261 ccgtcctgat caccgccgcc ggcctggcct cgatgggcca cagcgtcacc cgcatcccgg
     1321 tcgccgagat cgacggcatc cgcgtgatcg acctggcccc ggtccgcgtg tcgccgaccg
     1381 ccgaagacct gcgcaacgcc gcgctgaccg ccgtcaccac caccgtcggc accccggccg
     1441 tcaacggcct cggcggccac gccgacggcg ccaatccgct ggtcggggcg cagatggcca
     1501 tgccctacta ttcgttcggc aacaagatcg gccgcatcgg caaggactaa tttcatgacc
     1561 atcacctgga acgacagcgt cccgatctac cgccaactgc gcgaccgcgt cgtggcgatg
     1621 atcctggacg gcgccttgaa cgagggcgac ccgctgccgt cggtccggca ggtggcggcg
     1681 gatttccaga tcaatccgct gaccgtctcc aaggcctatc aggagctggt cgacgatcaa
     1741 ctggttgaaa aacggagggg gttgggcatg ttcgtcaacg atggagcgcg cgaggcactg
     1801 ctcaagtccg agcgcgagcg cttcctgcgc gaagagtggc ccgccctctt cgcccggctg
     1861 cagcggctgg gcctggacct gaagaccctg ctgcgcgagg cggccgacgg cccggagaaa
     1921 accgcatgaa cgccgtgatc accgctcgcg gtctgcgcaa gcaatacaag aacacgctgg
     1981 ccctggacga cgtgagcttt tccatcccgt ccgggcggat cgtcggcctg atcggcccca
     2041 acggcgccgg caagaccacg gcgctgaagg cgatcctcgg gctgacgccg ttcgagggcg
     2101 agctgcacgt gctcgggctg gacccgcgca cccagcgcaa caagctgatg gagcaggtgt
     2161 gcttcatcgc cgacgtggcc gtgctgccgc gctggatgcg cgtccgcgac gcgatcgact
     2221 tcg
//
LOCUS       DASVLL010000060         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_486692,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000060 DASVLL010000000
VERSION     DASVLL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486692"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF402_01040"
     CDS             <1..362
                     /locus_tag="VF402_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX7798901.1"
                     /translation="AFGQALKLALGDRAGMARFGFVLPMDETEARVSVDLGGRPFCVF
                     EGRFEGSHIGDYPTEMTAHAFRSLSETLGASIHVEVKGGNDHHKTEACFKALGRALRQ
                     ATRVEGDALPSTKGMLA"
     gene            362..958
                     /gene="hisH"
                     /locus_tag="VF402_01045"
     CDS             362..958
                     /gene="hisH"
                     /locus_tag="VF402_01045"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7798902.1"
                     /translation="MTDEIVVIELGCANTASVLFALERLGAQARLSSDAGEISAAKKV
                     VLPGVGAAGFAMRRIHELGLYDVIRGVRAPLLGVCLGQQLLFEGSDEGDVAGLGLIPG
                     RVTKMEVPADLVVPHMGWNQLAVLRDDPLTAGVKDGDYAYFVHSFVCPVNDCTLATSA
                     YGQPFAAMVRNENVWGCQFHPERSSAVGAQILKNFVNL"
     gene            955..1686
                     /gene="hisA"
                     /locus_tag="VF402_01050"
     CDS             955..1686
                     /gene="hisA"
                     /locus_tag="VF402_01050"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX7798903.1"
                     /translation="MILYPAIDLINGECVRLAQGRFDAVTKYDSDPFKRLDLFNAEHA
                     QWVHIVDLDGAKAGSPQQHDLIGRLAKASKAKIQTGGGVRSREHVQTLLDAGVSAVVV
                     GSAAVKDPDEVRGWLSDFGKDRITLALDVLPTKDGDFDAALHGWVEGSGISLWDVLDY
                     YPVGVAQRILVTDVSRDGMLMGPNMELMRALRHKRPDLEIQASGGVRSVEDLYDLKAL
                     GVHGAIVGKAIYEGLIDLKAALNVG"
     gene            1857..2624
                     /gene="hisF"
                     /locus_tag="VF402_01055"
     CDS             1857..2624
                     /gene="hisF"
                     /locus_tag="VF402_01055"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX7798904.1"
                     /translation="MLARRIIPCLDVKDGKVVKGVQFLGHEVLGDAVDLALRYRDEGA
                     DELVLYDITASSEGRTVDYDWVRDIARALDIPFCVAGGIRSAEQAVRCLDSGADKVSI
                     NSPALERPDLIDELAEMAGSQCVVVGVDSREENGDYIVHQYSGDPDKMRTVGRRTLDW
                     IVEAQTRGAGEIVLNCMNQDGVRKGYDLKQLRLARDLLHIPLVASGGAGAPEHFTEVF
                     DKADVSGALAASVFHKKIIAIPDLKRDLRVAGIPVRL"
     gene            2742..>3062
                     /locus_tag="VF402_01060"
     CDS             2742..>3062
                     /locus_tag="VF402_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX7798905.1"
                     /translation="MSESHALPNPLTVADIDRIDFAKDGGLVPAVVQHADTLQVLMLG
                     YMNAEALKATLQGGRVTFWSRSRGRLWMKGETSGDTLELDRVLADCDEDALLVYARPN
                     GPTCH"
BASE COUNT          503 a          993 c         1043 g          523 t
ORIGIN      
        1 tggccttcgg tcaggcgctg aaactggcgc tgggcgaccg ggcgggcatg gcgcgcttcg
       61 gcttcgtgct gccgatggac gagacggagg ccagggtcag cgtcgatctg ggcgggcgtc
      121 cgttctgcgt tttcgagggc cggttcgaag gctcgcacat cggcgactat ccgaccgaga
      181 tgaccgctca cgccttccgc tcgctgtcgg aaaccctggg cgcgtcgatc catgtcgagg
      241 tcaagggcgg caacgaccac cacaagaccg aagcctgctt caaggcgctg ggccgcgcgc
      301 tgcggcaggc gacgcgcgtc gaaggcgacg cgctgccgtc caccaagggg atgctggcgt
      361 aatgacggat gagattgtcg ttatagaact gggttgcgcc aatacggcct cggtgctgtt
      421 cgcgctggaa cgtctgggcg cgcaggcgcg gctatcttcg gacgcgggcg agatttctgc
      481 ggcgaagaag gtggtgctgc ccggcgtcgg cgcggcgggc ttcgccatgc gccgcatcca
      541 tgagcttggg ctttacgacg tcattcgcgg cgtcagggcg ccgctgctcg gcgtctgcct
      601 cggtcagcaa ctcttgttcg aaggctccga cgagggcgac gtcgcgggac tcggcctgat
      661 ccccggccgg gtgacgaaga tggaggttcc cgccgacctc gtcgtgccgc acatgggctg
      721 gaaccagctc gccgtgctgc gcgacgatcc gctcaccgcg ggcgtcaagg acggcgacta
      781 cgcctatttc gtccactcct tcgtctgccc ggtcaatgat tgcacattgg cgacctccgc
      841 ctacggccag cctttcgccg ccatggtccg caacgagaac gtctggggct gccagttcca
      901 cccggagcgc tcatccgccg tcggcgctca aatcctgaag aattttgtga acctatgatc
      961 ctctatcccg ccatcgacct gatcaacggc gaatgcgtcc gcctcgcgca ggggcgtttc
     1021 gacgccgtga cgaaatacga ctccgatccg ttcaaacgcc tcgacctgtt caacgccgag
     1081 catgcgcaat gggtgcatat cgtcgatctc gacggcgcca aggcgggttc gccgcagcag
     1141 cacgacctga tcgggcggct ggcgaaggcg tcgaaggcga agattcagac cggcggcggc
     1201 gtgcgttcgc gcgaacatgt ccagaccctg ctcgacgcgg gcgtctcggc ggtcgtggtc
     1261 ggctcggcgg cggtgaaaga cccggacgaa gtgcgcggct ggctgagcga tttcggcaag
     1321 gaccgcatca cgctggcctt ggacgtgctg cccactaagg acggcgattt cgacgccgcc
     1381 ctgcacggct gggtcgaagg ttcgggcata tcgttgtggg acgtgctgga ctattatccg
     1441 gtcggcgtcg cgcagcgcat cctcgtcacc gacgtgtcgc gcgacggcat gttgatggga
     1501 ccgaacatgg agctgatgcg cgccctgcgc cacaagcgcc cggaccttga aatccaggcg
     1561 tccggcggcg tgcgctcggt cgaggatctg tacgacctca aggcgctcgg cgtccacggc
     1621 gccatcgtcg gcaaggcgat ctacgagggg cttatcgacc tgaaggcggc gctcaatgtg
     1681 ggctaaagtt ctgacatcct cccctgcgca gcgcggaagg cagcgagcaa gcgaagcgcg
     1741 ccgggacgga gggggcgctc tcgcggccgg cctttggccc ctcacccgat cgccttcgct
     1801 tcgctcggct ctcgtcctct ccccacaggt ggggagaggg gaaggatatc ctctcaatgt
     1861 tggctagacg catcatcccc tgcctcgacg tcaaggacgg caaggtcgtc aagggcgtgc
     1921 agtttctcgg ccacgaagtg ctgggcgacg cggtcgacct cgcgctgcgc tatcgcgacg
     1981 aaggcgcgga cgagctggtg ctgtacgaca tcaccgcgtc cagcgagggc cgcaccgtcg
     2041 attacgactg ggtgcgcgac atcgccaggg cgctggatat tccgttctgc gtggcgggcg
     2101 gcatccgctc ggcggagcag gcggtgcgct gcctcgattc cggcgccgac aaggtgtcga
     2161 tcaattcgcc cgcgctggag cgccccgacc tgatcgacga actggccgag atggccgggt
     2221 cgcaatgcgt ggtggtcggg gtcgactcgc gtgaggaaaa tggcgactat atcgtccatc
     2281 aatattccgg cgacccggac aagatgcgca cggtgggccg ccgcacgctg gactggatcg
     2341 tcgaggcgca gacgcgcggc gcgggcgaga tcgtgctgaa ctgcatgaat caggacgggg
     2401 tgcgcaaggg ctacgacctg aaacagttgc ggctggcgcg cgacctgttg catatcccgc
     2461 tggtcgcctc cggcggcgcg ggcgcgcctg agcatttcac cgaggtgttc gataaggccg
     2521 atgtgtccgg cgcgctggcc gcctcggtgt tccacaagaa gatcatcgcc atccccgacc
     2581 tgaagcgcga ccttcgcgtg gcggggattc ccgtccggtt gtaaagacgc cccctccggc
     2641 gcgacaagcg cgccacctcc cccgctgcgc aggggaggat caaagacaac aaccccctca
     2701 tccctccccg tttacgggga gggggggaaa ggcccgaacc catgtccgaa tcccacgccc
     2761 tgcccaatcc cctcaccgtc gccgacatcg accggatcga cttcgctaag gacggcggcc
     2821 tcgtgcccgc cgtggtgcag cacgccgaca cgttgcaggt gctgatgctc ggctacatga
     2881 acgccgaggc gctgaaagcc acgctgcaag gcgggcgggt gaccttctgg tcgcgctcca
     2941 ggggccggtt gtggatgaag ggcgaaacct ccggcgacac gctggagttg gaccgcgtgc
     3001 tggccgactg cgatgaggac gcgcttctgg tttatgcccg cccgaacggc ccgacctgcc
     3061 ac
//
LOCUS       DASVLL010000061         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_272278,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000061 DASVLL010000000
VERSION     DASVLL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272278"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VF402_01065"
     CDS             <1..979
                     /locus_tag="VF402_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7798906.1"
                     /translation="PAFNVTWKLSDGRYRVIVAPKSLIEPWQARLLLGFGLTALLILP
                     LAYLLSQRLSRPITAFAEAAAKLSVEDSAPPVPADGPREVRQAAGVVNAMQTRIRKQI
                     ESRTMLMGAIAHDLKTPLARMRLRIEDLPAPVREKLSQDIAHMDGLIRSAMSFTSAHK
                     LAEQVRPLDLSALTESLIDDMGVVCEIGEASLAPNLMVMGDQVALTRILTNLIENAGR
                     YASGCRVRLAAQGDTVELSVLDDGPGLPEDALEAVFEPFYRLETSRNRDTGGTGLGLS
                     VARALAEAQGGTLSLRNRYDGDRVTGLEARLSLPRLRPKTRRKAAAATA"
     gene            1144..>1944
                     /locus_tag="VF402_01070"
     CDS             1144..>1944
                     /locus_tag="VF402_01070"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7798907.1"
                     /translation="MALRVAINGFGRIGRLVLRSIIEHGRKDIEVVAINDLGPVETNA
                     HLLRYDSVHGRFPAEVKVNGDELTIIANGATYGPIKVTAIRNPAELPHKALNVDIALE
                     CTGIFTAREKALAHIEAGAKKVIVSAPSDGADKTIVFGVNDDTLTADDVVISNASCTT
                     NCLAPVAKVLSDLCGIERGYMTTIHSYTGDQPTLDTMHKDLYRARAAALSMIPTSTGA
                     AKAVGLVLPHLKGKLDGSSIRVPTPNVSVVDLKIVPSRAVTKEEVNAAI"
BASE COUNT          341 a          676 c          635 g          292 t
ORIGIN      
        1 tcccgccttc aacgtgacgt ggaaactgtc cgacgggcgc taccgcgtca tcgtggcgcc
       61 gaaaagcctt atcgagccgt ggcaggcgcg gctgctgctc ggcttcgggc tgacggcgct
      121 gctgatcctg ccgctggcct acctgctgtc gcagcgcctg tcgcgcccga tcaccgcctt
      181 cgccgaagcc gccgccaagc tcagcgtcga ggacagcgcg ccgccggtcc ccgccgacgg
      241 cccgcgcgag gtgcgtcagg ccgccggcgt cgtcaacgcc atgcagacgc gcatccgcaa
      301 gcagatcgaa agccgcacca tgctgatggg cgccatcgcg cacgacctga agacgccgct
      361 ggcgcggatg cggctgcgca tcgaggatct gcccgcgccg gtgcgcgaaa agctgtcgca
      421 ggacatcgcg cacatggacg ggctgatccg ctcggccatg agcttcacca gcgcgcacaa
      481 gctggccgaa caggtgcggc cgctggacct gtcggccctg accgaaagcc tgatcgacga
      541 catgggcgtg gtgtgcgaga tcggcgaggc gagcctcgcg cccaacctca tggtcatggg
      601 cgatcaggtc gcgctgacgc gcatcctgac caatctgatc gagaacgccg gacgctatgc
      661 gtcgggctgc cgggtgcggc tggcggcgca gggcgacacg gtcgagctga gcgtgctcga
      721 cgacggcccc ggcctgcccg aggacgcgct ggaggcggtg ttcgagccgt tctaccggct
      781 ggagacgtcg cgcaaccgcg acaccggcgg cacggggctg ggcctgtcgg tggcgcgcgc
      841 gctggccgag gcgcagggcg ggacgctcag cctgcgcaac cgttatgacg gcgatcgcgt
      901 gaccggcctc gaagcgcgcc tcagcctgcc gcgcctgcgt ccgaagacgc gccgcaaggc
      961 cgcggcggcg accgcctgaa ccgtcttttc ggcaggccgg ataaggactt gatctggaat
     1021 tttcctgata gcgttaccat atagggtgcg gcgcgaaata gggcttgact tgccggccct
     1081 caggttgaag aaccggcgcc aacgtcacca taagacgaag tcagtcgaaa aggaattccg
     1141 cccatggctt tacgtgtcgc catcaatggt ttcggccgca tcggccgcct cgttctgcgc
     1201 tcgatcatcg agcatggccg taaggacatc gaagtcgtcg ccatcaacga tctcgggccg
     1261 gtggaaacca acgcgcacct gctgcgctac gacagcgtgc atggccgctt ccccgccgag
     1321 gtcaaggtca atggcgacga gctgaccatc atcgcgaatg gcgcgaccta cggcccgatc
     1381 aaggtgaccg cgatccgcaa tccggcggaa ctgccgcaca aggcgctgaa cgtcgacatc
     1441 gcgctggaat gcacgggcat cttcaccgcc agggaaaagg cgctggccca catcgaggcg
     1501 ggcgcgaaga aggtcatcgt ctcggccccg tccgacggcg ccgacaagac catcgtgttc
     1561 ggcgtcaacg acgatacgct gaccgctgac gacgtggtga tctccaacgc ttcgtgcacc
     1621 accaactgcc tggcgccggt cgccaaggtc ttgtcggacc tgtgcggcat cgagcgcggc
     1681 tacatgacca ccatccactc ctataccggc gaccagccga cgctggacac catgcacaag
     1741 gatctctacc gcgcccgcgc ggcggccctt tccatgatcc cgacctcgac cggcgccgcc
     1801 aaggccgtcg gcctcgtgct gccgcacctg aagggcaagc tggacggctc gtcgatccgc
     1861 gtgccgaccc cgaacgtctc ggtcgtcgac ctcaagatcg tgccgtcgcg cgccgtcacc
     1921 aaggaggagg tcaacgccgc catc
//
LOCUS       DASVLL010000062         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_3500, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000062 DASVLL010000000
VERSION     DASVLL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VF402_01075"
     CDS             <1..256
                     /locus_tag="VF402_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX7798908.1"
                     /translation="GLQAGVQSYSYAVFLMNDSALKYLHDSSGLEIGVGPNVVVVDQG
                     VARNLSTSTLKDDAYAFIFGQSGLMAGVALEGTKISKIKQ"
     gene            complement(258..1493)
                     /locus_tag="VF402_01080"
     CDS             complement(258..1493)
                     /locus_tag="VF402_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7798909.1"
                     /translation="MSDAAKNRNLTLILLLIVYILNFLDRQILSILKDPIAAELHLND
                     AQTGWLGGLPFALFFSVLALPAARWADRGSRVWLMTGALALWSAFTALCGLAGGYAQL
                     FLARMGVGVGEAGGVAPAYALIADLYPPSRRARAMSVYNLGIPIGGAAGILFGGLMAA
                     RVDWRWAFILTGLAGVAVAPVFRLLAREPERSAEAAAPSLTQTARDLARRPAFWLMAL
                     GASSASLVGYGFLYWFPSFLARSLSMDLSSRSVLFSAAFLISGLIGIGGSGWLADRLG
                     PARRSAYALIPAAALLIAAPLYALAVMAHTPRQAFLLILTPQALGLMWLSPVLTAVQH
                     MAPVRARAQASAVFLLINTLIGLILGPYFFGKVSDLLKASCGADSIKWAFVTGLVFYL
                     LAAALLALAARRMKADWID"
     gene            1621..2055
                     /locus_tag="VF402_01085"
     CDS             1621..2055
                     /locus_tag="VF402_01085"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7798910.1"
                     /translation="MIRTAKAHWAGNGQQGEGKLTTQSGVLNDQPYSFRLRFENADGK
                     AGTNPEELIAAAHSGCFSMALAFALAASGFTADSIDAEAQVSLQPADGGFAVTGITLK
                     LSAKIPNISNDQFQDIAAGAKANCPISKALAAVPITLEASLA"
     gene            complement(2052..3437)
                     /locus_tag="VF402_01090"
     CDS             complement(2052..3437)
                     /locus_tag="VF402_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7798911.1"
                     /translation="MFARLKAKLKAIPCLPLIVVLTGLRLIAAARAGLAPDESYYWLW
                     AQHPAFGYYDHPPMAAWWIGAFTFACKSALCVRLPFVLSFIAVCWLVYDAGKALFDAA
                     VARRALLWLNACLLLGVGTVLATPDPPSVLMWAGGLWALARLVKSGDGRWWLVFGLFA
                     GLGVLAKYTNLFLGLGVLLWLAADRDARRWLASPWLWLGGLVALAVMAPNLVWNAQHG
                     WITVAKQFGRIEASHFTPRYLLEFLISQPLLLNPFVAAFAGLAVMRGFRAKTQAGSLL
                     IALPAPLLAYMLIHVFHDRIQGNWPAPTFPGLVLLAASAGDAWPLARRAAAPFGVGVS
                     ALVLILLACGAAKQTEGWDQLAQRIDAARHATGAAWIATTDYDMQGELSYHLPAATVA
                     ATAERARYAWADRDPAGKALIVVAAGHTPALDRCFSDVQALGTVTRAASHKGDARLYT
                     GVPRAPGCDTD"
     gene            complement(3469..>3894)
                     /locus_tag="VF402_01095"
     CDS             complement(3469..>3894)
                     /locus_tag="VF402_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX7798912.1"
                     /translation="LKRPAVTLALIGAAAMAAAVALYGVCPIIKNIWTPTYAVFAGGA
                     SLLALCLLEPVADTPPVFPLRLYGGNPLLAYILCFLISPAFDMPCPPGGQSLRAAAQN
                     LFARAFEPHFASLLFGLIYVTLMCLLLWVCHRKRWFLKL"
BASE COUNT          725 a         1304 c         1371 g          494 t
ORIGIN      
        1 gggcctgcag gcgggggtgc agtcctattc ctacgcggtg ttcctgatga acgacagcgc
       61 gctgaaatac ctgcacgatt cgtcggggct ggagatcggc gtcggcccga acgtggtcgt
      121 ggtcgatcag ggcgtggcgc gcaacctgtc gacatcgacg ctcaaggacg acgcctatgc
      181 cttcatcttc ggccagtcgg gcctgatggc gggcgtggcg ctggaaggca ccaagatttc
      241 caagatcaag cagtagctta atcgatccag tcggccttca tgcgccgcgc ggccagcgcc
      301 agcaacgccg ccgccagcag gtagaagacc aacccggtca cgaaggccca cttgatcgaa
      361 tcggcgccgc acgacgcttt cagcaggtcg gaaaccttgc cgaaaaagta cgggccgagg
      421 atcaggccga tcagggtgtt gatcagcagg aacacggcgc tggcctgcgc gcgggcgcgg
      481 accggcgcca tgtgctgcac ggcggtcagc accggcgaca gccacatcag gcccagcgcc
      541 tgcggcgtca ggatcagcag gaaggcctgc cgcggcgtat gcgccatcac ggccagcgca
      601 tagagcggcg cggcgatcag cagggcggcg gcggggatca gcgcataggc gctccggcgc
      661 gccggaccca gccggtcggc cagccagccg ctgccgccga tgccgatcag gccgctgatc
      721 aggaacgccg ccgagaacag cacggaacgg ctggataaat ccatgctcag gctgcgcgcc
      781 agaaagctgg ggaaccagta gaggaagccg tagccgacca gcgaggccga ggacgcgccc
      841 agcgccatca gccagaaggc cgggcggcgc gccaggtcgc gcgcggtctg ggtcagagac
      901 ggcgccgccg cctccgcact gcgttcgggc tcgcgcgcca gcagccggaa cacgggcgcg
      961 acggcgaccc cggcaagccc ggtgaggatg aacgcccagc gccagtcgac gcgcgccgcc
     1021 atcagcccgc cgaacaggat gcccgccgcg ccgccgatgg ggatgcccag attatagacg
     1081 ctcatggcgc gggcgcggcg cgacggcgga tagaggtcgg cgatcagcgc ataggccggg
     1141 gccacgccgc ccgcttcgcc gacgccgacg cccatgcgcg ccaggaacaa ctgcgcgtaa
     1201 ccgcctgcca gcccgcacag cgccgtgaac gccgaccata gcgccagcgc cccggtcatc
     1261 agccagacgc gcgatccccg gtcggcccag cgcgcggcgg gcagggccag caccgagaag
     1321 aacagcgcga acggcaggcc gcccagccag ccggtctgcg cgtcgttgag atgcagttcg
     1381 gcggcgatgg gatccttgag gatgctcagg atctgccggt cgaggaagtt gaggatatag
     1441 acgatcagca gcaggatcag cgtcaggttg cggttcttgg cggcgtcgga catgtcggct
     1501 ccccgttttg tgatggtctt ctaaccgttt gctgagcttt gggccaccgt ttcaccgttg
     1561 tgaaaaacgg cgttcgggct tacgcctgac ggatcgcgtc atcagcacag gagacttccc
     1621 atgatccgca cggcaaaagc gcactgggcc ggcaacggcc agcagggcga gggaaaactg
     1681 accacccaga gcggcgtgct taacgaccag ccctacagtt tcaggctgcg tttcgagaac
     1741 gccgacggca aggcgggcac gaacccggag gaactgatcg cggcggcgca ttcgggctgt
     1801 ttttcgatgg cgctggcctt cgcgctggcg gcgtcgggct ttacggcgga cagcatcgac
     1861 gccgaggcgc aggtcagcct gcaaccggcg gacggcggct ttgcggtcac cggcattacg
     1921 ctgaagctga gcgcgaaaat cccgaacatc agcaacgacc agtttcagga catcgccgcc
     1981 ggggccaagg ccaattgccc gatctccaag gcgctggccg ccgtgccgat cacgctggag
     2041 gcgagcctgg cctaatcggt gtcgcagccg ggcgcgcgcg gaacgccggt atagaggcgc
     2101 gcgtcgccct tgtgcgatgc cgcgcgcgtc acggttccga gggcctgcac atcgctgaag
     2161 cagcggtcga gcgcgggcgt atggcccgcc gcgaccacga tcagcgcctt gcccgccgga
     2221 tcgcggtccg cccaggcgta gcgcgcgcgc tcggccgtgg cggcgaccgt tgcagcgggc
     2281 aggtgatagc tcagttcgcc ctgcatgtcg taatcggtgg tggcgatcca cgccgcgccc
     2341 gtcgcgtggc gggcggcgtc gatgcgctgc gccagttggt cccagccctc ggtttgcttc
     2401 gccgcgccgc acgccagcag gatcaggacc agcgccgaga cgccgacgcc gaacggcgcg
     2461 gcggccctgc gcgccagtgg ccacgcatcg cccgccgacg cggccagcag cacgaggccg
     2521 ggaaaggtcg gcgcgggcca gttgccttga atacgatcgt gaaagacgtg gatcagcatg
     2581 taggccagca gcggcgcggg cagggcgatc agcagggagc ccgcctgcgt tttggccctg
     2641 aacccgcgca tgacggccag tccggcgaag gcggcgacga acgggttgag caacaagggt
     2701 tgcgagatca ggaactccag caggtagcgc ggcgtgaaat ggctggcctc gatgcggccg
     2761 aactgcttgg cgacggtgat ccagccgtgc tgcgcgttcc agacgaggtt cggggccatg
     2821 acggccagcg cgaccagccc gcccagccat agccacggcg acgccagcca gcggcgcgcg
     2881 tcgcggtcgg cggccagcca cagcagcacg ccgagaccga ggaacagatt ggtgtacttg
     2941 gccagaaccc ccagccccgc gaacaggccg aagaccagcc accagcgccc atcgccgctt
     3001 ttgaccagcc gcgccagcgc ccacagaccg cccgcccaca tcagcacgct gggcggatcg
     3061 ggcgtggcca gaaccgtccc gacgccgagc agcaggcagg cgttgagcca caaaagcgcc
     3121 cgccgcgcga ccgccgcgtc gaacagggcc ttgcccgcgt catagaccag ccagcacacg
     3181 gcgatgaagc tcagcacgaa cggcaggcgc acgcacaggg ccgacttgca ggcgaaagtg
     3241 aaggcgccga tccaccacgc cgccatcggc ggatggtcgt aatagccgaa ggccggatgt
     3301 tgcgcccaca gccagtaata gctttcgtcc ggcgccagcc ccgcccgcgc cgccgcgatc
     3361 aggcgcaggc cggtcaggac cacgatcagc ggcaggcagg gtatggcttt cagtttggct
     3421 ttcaggcgcg cgaacatgcg cccttgtggc cggtttcgcg gcgcgagtct agagtttgag
     3481 gaaccagcgc ttgcggtggc aaacccacaa aagcaggcac atcaaggtca cgtagatcag
     3541 cccgaacagc agcgaggcga aatgcggctc gaacgcccgc gcgaacaggt tttgcgcggc
     3601 ggcccgcaag ctctggccgc cgggcgggca cggcatgtcg aaggcgggcg agatcagaaa
     3661 acagaggatg taggccagca gcggattgcc gccgtagagg cgcagcggaa agaccggcgg
     3721 cgtatcggcg accggctcca gcaggcacag ggccagcagc gacgcgccgc ccgcgaagac
     3781 ggcgtaggtc ggcgtccaga tgttcttgat gatcgggcag acgccgtaaa gcgccacggc
     3841 ggcggccatc gccgccgcgc cgatgagggc cagggtgacg gcggggcgct tcag
//
LOCUS       DASVLL010000063         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_379181,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000063 DASVLL010000000
VERSION     DASVLL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379181"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF402_01100"
     CDS             complement(<1..212)
                     /locus_tag="VF402_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX7798913.1"
                     /translation="MKDSRPAPPLDPSLTLPCVILDRPQLADNIGSVARVMANFGLQE
                     LRLVNPRDGWPQERAWAMSSGAFWPL"
     gene            complement(257..547)
                     /locus_tag="VF402_01105"
     CDS             complement(257..547)
                     /locus_tag="VF402_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798914.1"
                     /translation="MHLNVNVAAEVGIGLALWFGFIISMAITNGLLTEMRMDIEGDDY
                     RWRWPPPGLFQVTRAYRARHPGTKKPRQMWFANLTAVLCMVAVIVLVWQAGR"
     gene            674..1144
                     /locus_tag="VF402_01110"
     CDS             674..1144
                     /locus_tag="VF402_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7798915.1"
                     /translation="MTQPAVHAAFVIEKTYPHPLARVFAAFADPELKSRWFNPGHRQP
                     DASDEHDFRVGGVERTSWLMDETTPFPGAPMTRVSLYLDIVPEARIVTGSNMAMNGRV
                     FSSSLLTFEFSAEGEGARLILTHQGAFFEHADGPALREDGWRKVLEKLGESLAA"
     gene            1134..1448
                     /locus_tag="VF402_01115"
     CDS             1134..1448
                     /locus_tag="VF402_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7798916.1"
                     /translation="MPPDIARVFHALGDPTRRALIEAVTDAPLSVSKLAERLSVTLTA
                     VGQHLQVLEEAGLVRTEKLGRTRTCRLAQPGLLALEGWLRARRSPLEQALDRLGDVLD
                     EG"
     gene            1487..2632
                     /locus_tag="VF402_01120"
     CDS             1487..2632
                     /locus_tag="VF402_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7798917.1"
                     /translation="MPIPRLCLSLRIALLCGFAPFCGFVNAAAQPVHVGAALTTDFAA
                     NTQGGLRIGGMSMDNLDLTADWRPAPGWEAFGYVLVDAHGGFSARYTGDAQTVSNIDA
                     VPGTRLFEAWLRRTSGDGRHVTTFGLINLNGLFDVQPTGSLFLNASPGIGPDYSQSHP
                     SIFPVSALGVTHEWRPGGGWQARLGLFDGEAGDPRNPAVFTSLSLSARQGYHAVAEVE
                     RDFDDGFVKAGVWGYNRGLARLDGSGDGPRRGGYVQLGRTFTQEAAHPDQGLAGWVRL
                     GTADGAVFPVQDYAGGGFVYTGLLPGCDQDRAGLAVYSARYGAGWRRVTPGARAAETT
                     FEASYQRLPRAGLLLQPDVQYIRHPAAAAGVRDALAVILRVKLSTPG"
     gene            2742..>2971
                     /locus_tag="VF402_01125"
     CDS             2742..>2971
                     /locus_tag="VF402_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX7798918.1"
                     /translation="MAKIKVANPVVDIDGDEMTRIIWQLIKDKLIHPYLDIDLKYYDL
                     GMENRDATDDQVTVDAANAIKQYGVGVKCATIT"
BASE COUNT          516 a         1023 c          921 g          511 t
ORIGIN      
        1 tccagcggcc agaaggcgcc cgacgacatc gcccacgccc gctcctgcgg ccagccgtcg
       61 cgcggattga ccagccgcag ttcctgcaag ccgaaattgg ccatcacgcg cgccaccgag
      121 ccgatattgt cggccagttg cggacgatcg aggatcacgc agggcagcgt caggctgggg
      181 tcgagcggcg gcgcgggtcg ggagtctttc ataggggcgg cttataaacg caacctgcgc
      241 cagatggaaa cggcctttat cggccagcct gccagacaag tacgataacg gccaccatgc
      301 acaaaactgc ggtcaggttc gcaaaccaca tttgacgtgg ctttttggtt ccgggatgcc
      361 gcgcacgata ggcgcgtgtc acttgaaata aaccaggagg cggccatcgc cagcgataat
      421 catcaccctc aatgtccatg cgcatttcgg ttagcagacc attcgtgatc gccatgctga
      481 tgataaagcc aaaccatagc gccagaccga ttccaacctc ggcagccacg ttgacattca
      541 gatgcatgtc gccccccaaa aaatgcctgc atcctacatc tttggatacg cctcctcaat
      601 actgaagttt ccacttgact attgcgcggc atgtggaata ctgaagccac aacttcacta
      661 tggagaagcc accgtgaccc aacccgccgt ccacgccgcc tttgtcatcg aaaagaccta
      721 tccgcatccg ctggccaggg tgttcgccgc cttcgccgac ccggagctga aaagccgctg
      781 gttcaacccc ggccaccgcc agcccgacgc cagcgacgaa cacgatttcc gcgtcggcgg
      841 cgtcgaaaga acaagctggc taatggacga aaccacgccg tttccgggcg cgcccatgac
      901 gcgcgtctcg ctctatctcg acatcgtgcc ggaggcgcgc atcgtcacgg gctcgaacat
      961 ggcgatgaac gggcgggtgt tttcctcatc gctgctgacc ttcgaattta gcgccgaagg
     1021 cgaaggcgcc cgcctgatcc ttacccatca gggcgccttc ttcgaacacg ccgacggccc
     1081 cgccctgcgc gaggacggct ggcgcaaggt gctggaaaag ctcggagaaa gccttgccgc
     1141 ctgatatcgc cagagttttt catgcgctcg gcgacccgac gcggcgcgcg ctgatcgaag
     1201 ccgtgacgga cgcgccgctg tccgtgtcga aactggccga acgcttaagc gtcaccctga
     1261 ccgccgtcgg ccagcatttg caggttctgg aggaggccgg tctggtgagg accgagaagc
     1321 tgggccgcac ccgcacctgc cggctggcgc agcccggcct cctcgcgctc gaaggctggc
     1381 tgcgggcgcg ccgttcgccg ctggagcagg cgctcgaccg gctgggcgac gtgctggacg
     1441 aaggctgagt tgcaaaatct tcaccggttc cgtgtaaggc tcggccatgc cgataccgcg
     1501 cctctgcctc agcttacgga tcgccctgct ctgcggcttc gcgccgtttt gcggcttcgt
     1561 caacgccgcg gcgcagcccg tgcatgtcgg cgcggccctg accaccgatt tcgccgccaa
     1621 tacgcagggc ggcctgcgca tcggcggcat gagcatggac aatctcgacc tgaccgccga
     1681 ctggcgcccc gcgcccggct gggaagcctt cggctatgtg ctggtcgacg cgcatggcgg
     1741 tttcagcgcg cgctatacgg gcgacgcgca gaccgtctcc aatatcgacg cggttccggg
     1801 gacgcgcctg ttcgaggcgt ggctgcggcg cacgtccggc gacggccgcc acgtcactac
     1861 tttcggcctg atcaatctca acggcctgtt cgacgtgcag ccgaccggca gcctgttcct
     1921 caacgcctcg cccggcatcg gccccgacta ttcgcagtcg catccgtcga tcttccccgt
     1981 ctccgcgctc ggcgtcacgc acgaatggcg gcccggcggc ggctggcagg cgcgtttggg
     2041 cctgttcgac ggcgaggcgg gcgatccgcg caaccccgcc gtcttcacca gcctcagcct
     2101 gtcggcgcgt cagggctatc acgccgtcgc cgaggtcgag cgcgatttcg acgacggctt
     2161 cgtcaaggcg ggcgtttggg gctataaccg cggtctggcg cggctggacg gttccggcga
     2221 cggcccgcgc cgcggcggct atgtccagct tggccgcacc ttcacgcagg aagcggcgca
     2281 cccggatcag gggctggcgg gctgggtgcg gctgggcacg gcggacggcg cggtgtttcc
     2341 ggttcaggac tatgccggcg gcggcttcgt ctataccggg ctgctgccgg gctgcgatca
     2401 ggaccgcgcc ggactggccg tgtacagcgc ccgttacggc gccggctggc ggcgcgtcac
     2461 gcccggcgcg cgcgccgccg aaacgacctt cgaggccagc tatcagcgcc tgccgcgcgc
     2521 gggcctcctg ctgcaaccgg atgtgcaata tatccgccat cccgccgccg ccgccggggt
     2581 tcgcgacgcg ctggccgtca tcctgcgcgt gaagctgtcg acgcccggct aaaaccgttt
     2641 tgaccgcacc cgcttttcgt ttatacggct tggcgttcgc cgcccgctcc gaccctgtgc
     2701 gtcctgctgg caggcccggt tctgaaaaag gaatatctca catggctaaa atcaaggtcg
     2761 ccaatccggt cgtcgacatc gacggcgacg aaatgacccg catcatctgg cagctcatca
     2821 aggacaagct gatccacccg tacctcgaca tcgacctgaa atactacgat ctgggcatgg
     2881 agaaccgcga cgccaccgac gatcaggtga ccgtggacgc cgccaacgcg atcaagcagt
     2941 acggcgtcgg cgtcaagtgc gcgaccatca c
//
LOCUS       DASVLL010000064         5309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_379199,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000064 DASVLL010000000
VERSION     DASVLL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379199"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VF402_01130"
     CDS             <1..822
                     /locus_tag="VF402_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7798919.1"
                     /translation="FLQIPAFLVGFGVLAACCLPLPWPTRPGAYFKGAFVAPVADVFK
                     RYENWAAFILALILVYRVSEFILNIMNPFYVDLGFSLDTIAGMRKVWGVVMSMIGLGF
                     ASWIIARFGLMRSLVIGAVVGPLSHAGFMWLACAGHDIRVFAIAMAMDNISAAVSGTV
                     LIAYMSSLTSAPFTASQYALFSSLYSILGKLVASQSGRIVEASARAADHGGVAGLFTG
                     LMGHLHSDSYVAPAAKLGVTPAALGAGYFTFFAYTIVIGFVSVAMSVWLYLRRKT"
     gene            complement(819..3104)
                     /locus_tag="VF402_01135"
     CDS             complement(819..3104)
                     /locus_tag="VF402_01135"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX7798920.1"
                     /translation="MTTEKQTFSDEEALNLHQYPQPGKIALAPTKPMATQRDLALAYS
                     PGVAVPVRRIAENPDAAYDYTAKRNMVAVISNGTAILGLGNLGALASKPVMEGKSVLF
                     KRFADIDSFDIEVATQDPDEFITVVKNITAAFGGINLEDIKSPECFIIESALQDMADI
                     PVFHDDQHGTAIICAAGLINALEITGKRIEDIKVVLCGAGAAGLSSLSLVKALGVRKE
                     NTTVVDIHGVVYEGRGVDMDQWKSVHATATDKRTLAEAMVGADVFLGMSAKGVLTPEM
                     VAGMAADPIIFAMANPDPEIAPEEVAKVRSDAIVATGRSDYPNQVNNVLCFPYIFRGA
                     LDVRARRVNDDMKLAAARALAQLAREDVPDEVASAYQGRHLKFGKDYIIPSPFDPRLI
                     WYVPPFVSQAAMDTGVARKPIDDMDAYRKRLEQRLDPSAGFLQHITSTVRSRPPRRIV
                     FAEGEDIRVIRAAHAFKLQGLGTPILCGRENLIEENMRAAGIDPAEEGIEIINARLSH
                     RNAAYAQLLYSRLQRSGYLKRDVDRLINLDRNSFAAAMLASGHADGMVTGVTRAFDQA
                     LTDVLRVVDPAPDGRIMGLSIVLAKGRTLFIADTTVAETPSPEELGDIAVEAAMAVRA
                     MGHTPRVAFMSYSTFGNPSGERSERVKQAVSLLEQRGVDFEFEGEMPPDVALDPARWT
                     NYPFQRLTAPANVLVMPAIQSASISTKLIEALGGATVVGPLLLGLSKPVQICQLSDSV
                     SKILTMATFAAYDVRATSTRV"
     gene            3379..>5309
                     /gene="mutS"
                     /locus_tag="VF402_01140"
     CDS             3379..>5309
                     /gene="mutS"
                     /locus_tag="VF402_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7798921.1"
                     /translation="MNASQPLADSALDLEGATPVMAQYLQTKAQHPDALLFFRMGDFY
                     ELFFEDAVTAAQALSLALTRRGQFRGEDIPLAGVPFHAADAYIAKLIRQGYRVAVCEQ
                     LEDPAEAKKRGSKSVVKRGIVRVVTPGTLTEETLLEERGANRLVAIASRGGVMALGMV
                     ELSTGDVEVAEIAPEQLPAQLAALRPSESLVADRVLQDEDLYAQVKLYGGVIQPQAPS
                     LSDPAAGETRLLKLYGVQTLDGFGAFSTAEVSALGMLAAYIDVTQAGKIPALKPPARV
                     ISNNHLSIDAATRNSLEIDRTQSGRREGSLIAALDCTVTSAGGRLLLSRLSRPLCRPD
                     AIHARLDAVAWLIERRDLRMALRQQMRSLADQARALSRLSLNRGGPRDLVVIRDALAI
                     GETLAARLNDACHRADPDLIASCPAEIEAVITALIPSPSLSALRERLARAVVDEPPLS
                     LRDGGYIREGFHADLDEARSLRDDSRQIILALEQRLAAETGIAIRIKFNNVLGYFIEL
                     SARLAEQLQAQDAQKQFIHRQSLANQVRFVTTELVELDGRIQRAAAQGLALEQEIFES
                     LRDQVRDNAAGIQAAHDAVCALDVACANAHWAEDHEATRPVVDDSTVLEISRGRHPVV
                     AAALKAQGKPFTPNDVELDA"
BASE COUNT          817 a         1763 c         1791 g          938 t
ORIGIN      
        1 ttcctgcaaa tcccggcctt tctggtcggc ttcggcgtgc tggcggcgtg ctgcctgccg
       61 ctgccgtggc cgacgcggcc cggcgcctat ttcaagggcg ccttcgtcgc gccggtggcc
      121 gatgtcttca agcgttacga aaactgggcg gccttcattc tggcgctgat cctcgtttat
      181 cgggtcagcg agttcatcct caacatcatg aacccgttct atgtcgatct cggcttcagc
      241 ctcgacacga tcgccgggat gcgaaaggtc tggggcgtgg tcatgtcgat gatcggctta
      301 gggttcgcca gctggatcat cgcccgtttc ggcctgatgc ggtcgctggt catcggcgcg
      361 gtggtcgggc cgctcagcca tgcgggcttc atgtggctgg cctgcgccgg acatgacatc
      421 agggtgttcg ccatcgccat ggcgatggac aatatttcgg ccgccgtctc cggcacggtg
      481 ctgatcgcct acatgtcgtc gctgacctcg gcgccgttca cggcgtcgca atacgcgctg
      541 ttctccagcc tctattcgat cctcggcaag ctggtcgcgt cgcagtcggg gcgcatcgtc
      601 gaggcttcgg cccgcgccgc cgatcacggc ggcgtcgccg ggctgttcac cggcctgatg
      661 ggccatctgc acagcgattc ctacgtcgcg cccgccgcga aactgggggt gacgcccgcc
      721 gcgctggggg cgggctattt caccttcttc gcctacacca tcgtgatcgg cttcgtgtcg
      781 gtggcgatga gcgtgtggct ctacctgcgg cgcaaaacct agacacgggt gctggtggcg
      841 cgcacgtcgt aggcggcgaa ggtcgccatg gtcaggattt tcgacaccga atccgacaac
      901 tggcagatct gcacgggctt cgacagaccc agcagcagcg ggccgaccac ggtggcgccg
      961 cccagcgcct cgatcagctt ggtcgagatc gaggccgact ggatggcggg catgaccagc
     1021 acgttggcgg gcgcggtcag gcgctggaac ggatagttgg tccagcgcgc cgggtccagc
     1081 gccacgtcgg gcggcatttc gccctcgaat tcgaaatcga cgccgcgctg ctccaaaagg
     1141 ctcacggcct gcttgacgcg ctcggagcgc tcgcccgacg ggttgccgaa ggtcgaatag
     1201 ctcatgaagg cgacgcgcgg cgtatggccc atggcgcgca ccgccatcgc cgcctcgacc
     1261 gcgatgtcgc ccagttcttc cggcgacggc gtttcggcga ccgtggtgtc ggcgatgaac
     1321 agggtgcggc ccttggccag cacgatggag aggcccatga tgcgcccgtc cggcgccgga
     1381 tcgaccacgc gcaacacgtc ggtcagcgcc tggtcgaagg cgcgcgtcac gccggtgacc
     1441 atgccgtcgg cgtggccgga cgccagcatg gcggcggcga acgagttgcg gtcgagattg
     1501 atcaggcggt cgacgtcgcg cttgagatag ccgctgcgtt gcaggcgcga atacagaagc
     1561 tgggcatagg cggcgttgcg gtgcgacagg cgcgcgttga tgatctcgat gccttcctcg
     1621 gccgggtcga tgcccgccgc gcgcatattc tcctcgatca ggttctcgcg gccgcacagg
     1681 atcggcgtgc ccagtccttg cagcttgaag gcatgggcgg cgcggatgac gcggatgtcc
     1741 tcgccctcgg cgaagacgat gcgccggggc ggacgcgaac gcacggtgga ggtgatgtgc
     1801 tgcaaaaagc ccgccgacgg atcgaggcgc tgctccaggc gcttgcgata ggcgtccatg
     1861 tcgtcgatcg gcttgcgcgc cacgccggtg tccatcgcgg cctgcgacac gaagggcggc
     1921 acgtaccaga tcaggcgcgg atcgaagggc gaggggatga tgtagtcctt gccgaacttc
     1981 aggtgacggc cctgataggc ggaggcgacc tcgtccggca catcctcgcg cgccagttgc
     2041 gccagcgcgc gcgcggcggc cagcttcatg tcgtcgttga cgcggcgggc gcgcacgtcc
     2101 agcgcgccgc ggaagatgta ggggaagcac aggacgttgt tgacctgatt gggatagtcc
     2161 gaccggccgg tggcgacgat ggcgtcggag cgcaccttcg ccacctcttc cggcgcgatt
     2221 tccgggtcgg gattggccat ggcgaagatg atcgggtccg ccgccatgcc cgccaccatc
     2281 tccggcgtca gcacgccctt ggccgacatg cccaggaaca cgtccgcccc gaccatcgcc
     2341 tcggccaggg tgcgcttgtc cgtggcggtg gcgtggaccg acttccactg gtccatgtcg
     2401 acgccgcggc cttcatagac gacgccgtgg atgtcgacga ccgtggtgtt ttccttgcgc
     2461 acccccagcg ccttgaccag agacagcgac gacaggcccg ccgcgcccgc gccgcacagc
     2521 acgaccttga tgtcctcgat gcgcttgccg gtgatttcca gcgcgttgat caggcccgcc
     2581 gcgcagatga tggccgtgcc gtgctggtcg tcgtgaaaga ccgggatgtc ggccatgtcc
     2641 tgcaaggccg attcgatgat gaaacattcc ggcgacttga tgtcttcgag attgatgccg
     2701 ccgaaggccg cggtgatgtt cttgaccacg gtgatgaatt cgtccggatc ctgcgtcgcg
     2761 acctcgatgt cgaagctgtc gatgtcggcg aagcgcttga acagcacgct cttgccctcc
     2821 atcaccggct tggaggccag ggcgccgaga ttgccgaggc cgaggatcgc cgtgccgttc
     2881 gagatcaccg ccaccatgtt gcgcttggcc gtatagtcgt aggccgcgtc ggggttctcg
     2941 gcgatgcggc gcaccggaac ggccacgccc ggcgaatagg ccagcgccag atcgcgctgc
     3001 gtcgccatcg gcttggtcgg cgccagcgca atctttcccg gttgcggata ctggtgcagg
     3061 ttgagcgctt cttcgtcgct gaaggtctgt ttctcggtgg tcatgcgatt gtgactttca
     3121 ctcggccggt ttgggtccgg ctcatcgggc tttttgtatg ataataaaag gcgttatcgc
     3181 gccaagtctt agaacaagat caatagctta taaaggcact aaatcttaca aaaggtttct
     3241 tttcgcgacg cggcgcgcct gccgtttctt ccgtaacggg aattaaattg tctgacaagt
     3301 cgcgggagcg gcttttttgc acatcttcgc gtccgcccgc cccttcccgg cgccgttaac
     3361 acaggttaac ctgcgcgcat gaacgcttcg cagcccctcg ccgattccgc cctcgatctc
     3421 gaaggcgcca cgcccgtcat ggcgcagtac ctgcaaacca aggcgcagca cccggacgcg
     3481 cttttgttct tccgcatggg cgacttctac gagctgttct tcgaggacgc ggtgacggcg
     3541 gcgcaggccc tgtcgctggc cctgaccagg cgcggccagt tccggggcga ggacattccg
     3601 ctggccgggg tgccgttcca cgccgccgac gcctatatcg ccaagctgat ccggcagggc
     3661 tatcgcgtcg cggtgtgcga gcagctggaa gacccggcgg aggccaagaa gcgcggctcc
     3721 aaatcggtgg tcaaacgcgg catcgtgcgc gtggtcacgc ccggcaccct gaccgaggag
     3781 acgctgctgg aagagcgcgg cgccaaccgt ctggtcgcca tcgcgtcgcg cggcggcgtt
     3841 atggcgctgg gcatggtcga gctgtccacc ggcgacgtcg aggtggccga gatcgcgccg
     3901 gagcagttgc ccgcgcaact ggccgccctg cgcccgtcgg aaagcctcgt cgccgaccgc
     3961 gtgttgcagg acgaagacct ctacgcacag gtcaagctct atggcggggt gatccagccg
     4021 caggcgccgt ccctgtccga tccggcggcg ggcgagacgc gcctgctgaa actctacggc
     4081 gtgcagaccc tggacggctt cggcgccttt tcgacggcgg aagtctcggc gctggggatg
     4141 ctggccgcct atatcgacgt gacgcaggcc gggaagattc ccgcgctcaa gccgcccgcg
     4201 cgcgtgatct ccaacaacca tctcagcatc gacgcggcga cgcgcaactc gctggagatc
     4261 gaccgcacgc aaagcggacg gcgcgagggc agcctgatcg cggcgctgga ttgcaccgtg
     4321 acctcggcag gcgggcggct cctgctgtcg cggctgtcgc ggcccctgtg ccggcccgac
     4381 gcgatccacg cgcggctcga cgcggtggcg tggctgatcg agcgccgcga cctgcgcatg
     4441 gcgttgcgcc agcagatgcg cagcctggcc gatcaggcgc gcgccctgtc gcggctgtcg
     4501 ctgaaccggg gcgggccgcg cgatctggtg gtgatccgcg acgcgctggc catcggcgaa
     4561 acgctcgccg cccggctcaa cgacgcctgc catcgcgccg atcccgacct gatcgcctcg
     4621 tgcccggccg agatcgaggc cgttatcacg gccctgatcc cgtcgccgtc gctcagcgcc
     4681 ctgcgcgagc ggctggcgcg cgccgtggtc gacgaaccgc cgctctcctt gcgcgacggc
     4741 ggctatattc gcgaaggctt ccacgccgat ctcgacgagg cgcggagcct gcgcgacgac
     4801 agccgccaga tcatcctcgc cctggagcag cggctggccg ccgaaaccgg catcgccatc
     4861 cgcatcaagt tcaacaacgt cctcggctat ttcattgagc tgtcggcccg gctggccgag
     4921 cagttgcagg cgcaggacgc gcaaaagcag ttcatccacc gccagagtct ggccaatcag
     4981 gtgcgtttcg tcaccaccga actggtcgag ctggacgggc gcatccagcg cgcggcggcg
     5041 cagggtctgg cgctggagca ggagattttc gagtccctgc gcgatcaggt gcgcgacaac
     5101 gccgccggga ttcaggccgc gcacgacgcc gtctgcgcgc tcgacgtcgc ctgcgccaac
     5161 gcgcactggg ccgaggacca cgaggcgacg cggccggtcg tcgacgattc cacggtgctg
     5221 gagatttcaa ggggacgcca cccggtcgtc gccgccgccc tgaaggcgca gggcaagccg
     5281 ttcacgccca acgacgtcga gcttgacgc
//
LOCUS       DASVLL010000065         5509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_218093,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000065 DASVLL010000000
VERSION     DASVLL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218093"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..707
                     /locus_tag="VF402_01145"
     CDS             132..707
                     /locus_tag="VF402_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798922.1"
                     /translation="MAKAVFHRHQRVFVEPVGTWAVVDKVNPVWVKGFAEPVRVTYDC
                     GLGRDFAAEELVAEADAETIDSDGFETRAAHWRLMRARNKWQTPEESAHHPFPGTYPV
                     IVTDENDWGGWRVPGAEYERDPNLIEMQARLIACAPKLLKLARDMIGYGRNNADFDDE
                     TIALIKRAEQICRAIDNDGVTAASPKSKVGA"
     gene            911..3538
                     /gene="pepN"
                     /locus_tag="VF402_01150"
     CDS             911..3538
                     /gene="pepN"
                     /locus_tag="VF402_01150"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX7798923.1"
                     /translation="MRTDTPQPIRLADYRAPDFRIPSIALSFDLHPTQTRVKARLGVE
                     RQGAKDAPLVLMGERLKLISVALDGRTLSDDAYVLTDETLSIADVPDAFTLETEVEID
                     PSNNKTLEGLYMSSGRFCTQCEAEGFRKITYAFDRPDSLSKYRVRIEAPKAGFPHLLS
                     NGNLIGQGDLPGDRHFAQWEDPFFKPCYLFALVAGELDVLEDRFVTVSGRAVDLRIYV
                     DTGMRARAAYAMDALKRAMKWDEDAYGREYDLDLFMIVAVRDFNFGAMENKGLNIFNA
                     SLLLADPQTATDADYERIESVIAHEYFHNWSGNRVTCRDWFQLCLKEGLTVFRDQGFS
                     ADQRGHAVARIKDVRALRARQFPEDSGPLAHPVRPSSYLKIDNFYTATIYEKGAEIVG
                     MLKTYVGPETYRKALDHYFAANDGTAATLEDFLASFEAVTGESFAPWLVWYTQAGTPR
                     LNVAAAYDAAAKTLTLNLDQATRPTPGQPDKTAVPVPVRFGLLTRDGRPQGFTLDGER
                     RDDTLYVLRDAAASLTLHEVAEPPVISALRHFSAPVILSLDEDADAAFARMQGDPDPF
                     NRWEAAQGLARDLILSGDLAQAARFGTALQACLTDAALEPAFQALMLALPSESDLAQA
                     RAPVNPRVLHDNRQALKAALSRQLDPVLRAAYDAVPRPNAFSPDARAAGGRALRNALL
                     DLIMAGYADSPDRPERLALAEAHFAQADNMTDMIGGLGALAYAEPEAFERALAAFYDR
                     FRTEPLVIDKWFALQSGSANADPARTRQRVRALIQHPDFDPKTPNRWRAVVQAFAGNP
                     AAFHAEDGSGYDVLVEQVLMVDAFNPMTAARLVEPLSRFRIYASPWREAMQAALTRIR
                     DAPGLSKNVLELSAKALDG"
     gene            3630..4199
                     /locus_tag="VF402_01155"
     CDS             3630..4199
                     /locus_tag="VF402_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7798924.1"
                     /translation="MASLERVRFLIVDDNAHMINIVKTILRGFGAKHIFDARDAAEGL
                     HSLKHDSVDIVITDYQMEVLDGIDFIKLLRNSSDSPNRYVPVIMLTAHSERSHVVAAR
                     DAGANEFCCKPVTALELRNKVAAVVDHPRPFIKTGNYFGPDRRRKLDPNYQGPERREG
                     WSQDHQPPSRPRRRSATADDMPGDWDDDL"
     gene            4300..>5509
                     /locus_tag="VF402_01160"
     CDS             4300..>5509
                     /locus_tag="VF402_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX7798925.1"
                     /translation="MATGRQNQYGDTAAARRKPGDPSPDTPESRRPQSRAGKRSRKVS
                     AFSIAANYQIAMAMPPWMRVGALSLSLGLAIFFALSTMQLGNSISADKDNEGRALLSD
                     AQLRAARLDARLSASRLALEAAQAQLDNHGDEPLSAAETAMKLARGRLSAVSVVGPKG
                     LLAQSGPDDAELVAAAASEATRPLDLVTASSRLIRTPRPYLTLRDGPFTLVGRLDNSL
                     AAPGDRVVSALIDRDGVVVEASDESLLGQSLKDAFAVTYSQLRARADSQDLIQGALKE
                     GGFLKIAVVRDDAGALDDDGLLLVRAQPSQRFYSPSASLTRAMFFVGGPLLIGLLFGL
                     LLFVQARKNRDDALQFQANEHRYKLAVESARCGIWDWDLEQGLVYMSDVTGAMFGWGG
                     GGVATTAEVLE"
BASE COUNT          967 a         1886 c         1781 g          875 t
ORIGIN      
        1 tttagatttc agagacttac cggcggcaaa aaagtttccc ggacccggac gcgcagccta
       61 aggggaaata ttaagctttc tcccgtaaaa agagcagacc gcgaccccga tagaagatca
      121 ggaaagacct catggccaag gccgtattcc accgtcacca gcgtgttttc gtcgaacccg
      181 tcggcacatg ggccgtcgtc gacaaggtca atcccgtctg ggtcaagggc ttcgccgagc
      241 cggtgcgcgt gacctacgac tgcgggctgg gccgcgactt cgccgccgag gagctggtcg
      301 ccgaggccga cgccgaaacc atcgattccg acggcttcga gaccagggcc gcgcactggc
      361 ggctgatgcg cgcgcgcaac aaatggcaga cgccggagga aagcgcccac catccgttcc
      421 ccggcaccta tccggtcatc gtcaccgacg aaaacgactg gggcggctgg cgcgtgccgg
      481 gcgccgaata cgagcgcgac ccgaacctga tcgagatgca ggcccgcctg atcgcctgcg
      541 cgcccaagct gctgaaactg gcgcgcgaca tgatcggcta cggccgcaac aacgccgatt
      601 tcgacgacga gaccatcgcc ctgatcaagc gcgccgaaca gatctgccgc gccatcgaca
      661 atgacggcgt caccgccgcc agcccgaagt cgaaggtcgg agcttagagc gcttttcgat
      721 ctgatggaat cagatcggcg ctctaagttt ttattttgtc gctcttcctt atccaaaaag
      781 cggaaccact ttttggggaa acgctctagg cgttttttgc ggtcttttga ccggctttta
      841 actggcggaa ccgatgcatg acgcctaaat cggtaaggaa ccgatttcag gccccgacac
      901 agggaagatc atgcgcacag acacgcccca gcctatccgc ctcgccgatt accgcgcgcc
      961 cgatttccgc atcccgtcca tcgccctgag cttcgacctg cacccgacgc aaacgcgggt
     1021 caaggcgcgg ctcggcgtgg agcgtcaggg cgccaaggac gcgccgctcg tgctgatggg
     1081 cgagcggctg aagctgatct cggtcgcgct ggacggacgg acgctgagcg acgacgcgta
     1141 cgtcctgacg gacgaaaccc tgagcatcgc cgatgtcccg gacgccttca cgctcgaaac
     1201 cgaggtcgag atcgatccgt cgaacaacaa gacgctggaa gggctttaca tgtcctccgg
     1261 ccgcttctgc acccagtgcg aggcggaggg cttccgcaag atcacctacg ccttcgaccg
     1321 cccggattcg ctcagcaaat accgcgtgcg catcgaggcg ccgaaggccg gttttccgca
     1381 ccttttgtcc aacggcaacc tgatcgggca aggcgacctg cctggcgatc gccatttcgc
     1441 gcaatgggaa gacccgtttt tcaagccctg ctacctgttc gcattggtgg cgggcgagct
     1501 ggacgtgctg gaggaccgtt tcgtcaccgt gtcgggacgc gcggtcgatc tgaggatcta
     1561 tgtcgacacc ggcatgcggg cgcgcgccgc ctacgcgatg gacgcgctga agcgggcgat
     1621 gaagtgggac gaggacgcct atgggcgtga atacgatctc gacctgttca tgatcgtggc
     1681 ggtgcgcgac tttaatttcg gcgccatgga gaacaagggg ctgaacatct tcaacgcctc
     1741 gctgctgctg gccgacccgc agaccgcgac cgacgccgac tatgagcgca tcgaaagcgt
     1801 gatcgcgcac gaatatttcc acaactggtc cggcaaccgc gtcacctgcc gcgactggtt
     1861 ccagctttgc ctgaaggagg gcctgaccgt gttccgcgat cagggctttt ccgccgacca
     1921 gcgcggccac gcggtcgcgc gcatcaagga cgtgcgggcc ttgcgcgcgc gtcagttccc
     1981 tgaggattcc ggccccctcg cccatccggt gcgcccgtcc tcgtacctga agatcgacaa
     2041 cttctacacc gcgaccatct atgagaaggg cgcggagatc gtcggcatgt tgaagaccta
     2101 tgtcggaccg gaaacctatc gcaaggcgct cgaccattat ttcgcggcga acgacggcac
     2161 ggcggcgacg ctggaggact tcctcgcgag cttcgaggcg gtgaccggcg aaagcttcgc
     2221 gccgtggctg gtctggtaca cccaggccgg gacgccgcgg ctgaacgtcg cagccgccta
     2281 tgacgcggcg gcaaagaccc tgacgctgaa ccttgatcag gcgacccgcc cgacgccggg
     2341 ccagccggac aagaccgccg tgccggtccc ggtccgcttc ggcctcctga cccgcgacgg
     2401 gcgtccgcag ggcttcacgc tggacggcga acgccgggac gacacgctgt acgtgctgcg
     2461 cgacgcggcg gcgagcctga ccctgcatga ggtcgccgaa ccgccggtga tctcggccct
     2521 gcgccatttc tcggcgccgg tcatcctgag cctcgatgag gacgccgacg ccgccttcgc
     2581 gcgcatgcag ggcgatcccg acccgttcaa ccgctgggag gcggcgcagg ggctggcgcg
     2641 cgacctgatc ctgtcgggcg atctggctca ggccgcgcgc ttcggcacag cgttgcaggc
     2701 gtgcctgacg gacgcggcgc tggagccggc gtttcaggcg ctgatgctgg ccctgcccag
     2761 cgaatccgat ctggcgcagg cgcgcgcgcc ggtgaatccg cgcgtgctgc acgacaaccg
     2821 gcaggcgctc aaggccgcgc tgtcgcgcca gctcgatccg gtgctgcgcg cggcttacga
     2881 cgccgttccg cgccccaatg cattcagccc cgacgcgcgc gcggcgggcg gccgcgccct
     2941 gcgcaacgcg ctgctggacc tgatcatggc cggttacgcc gacagccccg accgccccga
     3001 acggctggcc ctggccgagg cgcacttcgc gcaggccgac aacatgaccg acatgatcgg
     3061 cggcctcggc gcgctggcct atgccgagcc ggaggcgttc gagcgcgcgc tggccgcctt
     3121 ctacgaccgc ttcaggaccg agcctctggt gatcgacaaa tggttcgcgc tgcaatcggg
     3181 ctcggcgaac gccgatccgg cccggacgcg ccagcgcgtg cgcgccctga tccaacatcc
     3241 cgatttcgat ccgaagacgc cgaaccgctg gcgcgcggtc gtgcaggcct tcgcgggcaa
     3301 tcccgccgcc ttccacgccg aggacggctc cggctacgac gttctggtcg agcaggtgct
     3361 gatggtcgac gccttcaacc cgatgacggc ggcgcgcctc gtcgagccgc tcagccgctt
     3421 ccgcatctat gcatcgccgt ggcgcgaagc catgcaggcc gcgctgaccc gcatccgcga
     3481 cgcgccgggc ctgtcgaaaa acgtgctgga gctgagcgcg aaggcgctgg acgggtaagc
     3541 gccgccttaa ccattcttaa gccataaggc tttaggaacg actaaggttc ggaaggctgg
     3601 gcgcgagcgc agcctctcag gagagacgag tggccagcct ggaacgcgtg cggtttctga
     3661 tcgtggacga caacgcccat atgatcaata tcgtcaagac catcctgcgc ggcttcggcg
     3721 ccaagcacat cttcgacgcg cgcgacgccg ccgaagggct gcacagtctc aagcacgact
     3781 cggtcgacat cgtcatcacc gactatcaga tggaggtgct ggacgggatc gacttcatca
     3841 agctgttgcg caactcgtcc gacagcccga accgctacgt gccggtgatc atgctgaccg
     3901 cccattcgga gcgctcgcac gtcgtcgccg cgcgcgacgc cggggccaac gaattctgct
     3961 gcaagccggt gaccgcgctg gagctgcgca acaaggtggc ggcggtggtc gaccatccgc
     4021 gccccttcat caagaccggc aactatttcg gccccgaccg ccgccgcaag ctggacccca
     4081 attatcaggg gccggaacgc cgcgagggct ggagccagga tcaccagccg ccgtcgcgcc
     4141 cgcgccgccg cagcgcgacc gccgacgata tgcccggcga ctgggacgac gatctgtaat
     4201 cgtccttacg aatacggttt ataaaagctt aagactctcg acaatagatt aaccgcgcgt
     4261 ttaccctaac cgttaagcac gaatcatcag ggacaacccg tggcgacagg gcgtcaaaat
     4321 cagtacggcg acaccgccgc cgcccgccgc aagcccggcg acccgtcccc cgatacgcca
     4381 gaatcacgcc gcccgcagag ccgcgccgga aaacgctccc ggaaagtttc ggccttctcc
     4441 atcgccgcca attaccagat cgccatggcc atgccgccgt ggatgcgcgt cggcgcgctc
     4501 agcctgtcgc tggggctggc catcttcttc gccctgtcga ccatgcagct cggcaacagc
     4561 atctccgccg acaaggacaa tgagggccgc gccctgctca gcgacgcgca gttgcgcgcc
     4621 gcgcggctcg acgcgcgcct gtcggcgtcg cggctggcgc tggaggcggc gcaggcgcag
     4681 ctcgacaacc acggcgacga gccgctgagc gccgccgaaa cggcgatgaa gctggcgcgc
     4741 ggacgcctga gcgccgtgtc ggtcgtcggc cccaaggggc tgctggcgca aagcggcccg
     4801 gacgacgccg aactggtcgc ggcggcggcg agcgaagcga cgcgcccgct cgatctggtc
     4861 acggcgtcgt cgcgcctgat ccgcacgccg cggccctatc tgacgctgcg cgacggaccg
     4921 ttcaccctgg tcgggcggct ggacaacagt ctggccgcgc ccggcgaccg cgtcgtcagc
     4981 gcgctgatcg accgcgacgg cgtggtcgtc gaggcgtccg acgagagcct gctgggccag
     5041 tcgctgaagg acgccttcgc cgtcacctac agccagttgc gcgcccgcgc cgattcgcag
     5101 gatctgattc agggcgcctt gaaggaaggc ggctttctca agatcgccgt cgtgcgcgac
     5161 gacgccggcg cgctggacga cgacgggctg ctgctggtgc gcgcccagcc gagccagcgc
     5221 ttctattcgc cgtcggccag cctgacgcgc gccatgttct tcgtcggcgg gccgctgctg
     5281 atcgggcttc tgttcgggct tctgctgttc gtgcaggcgc gcaagaaccg cgacgacgcc
     5341 ttgcagtttc aggccaacga acaccgctac aagctggcgg tggaatcggc gcgctgcggc
     5401 atctgggact gggatctgga gcagggcctc gtctatatgt cggacgtgac cggcgccatg
     5461 ttcggctggg gcggcggggg cgtggcgacc acggccgagg tgctggagc
//
LOCUS       DASVLL010000066         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_272773,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000066 DASVLL010000000
VERSION     DASVLL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272773"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VF402_01165"
     CDS             <1..319
                     /locus_tag="VF402_01165"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX7798926.1"
                     /translation="GETTKADYQDVFIPAVDAALKTHDKVALYYETAPDFTGYSPGAM
                     GEDAVLGFRHYFQFSRVAILSDVGWIRGAVAAFRPLFPCPVRLYRPDEAAAARRWIET
                     GQD"
     gene            complement(404..>2157)
                     /locus_tag="VF402_01170"
     CDS             complement(404..>2157)
                     /locus_tag="VF402_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7798927.1"
                     /translation="LYGLFDGVDVRSEGLVDRFSTTFGQVNLNLTHRFSDRWAISAMI
                     GSSDSKYTSPLRLQTYMDAIDTDDFSLDFGGDNRGVPTIKFDGIDVANVANFNYAPAN
                     AAGDVLGGFSLQGRPLFNDTTNKTATFEVKYTANDTIAVKLGAEGRVEDFNSHALSLT
                     PGQAATQSGVDLAGITTTITGMDDLWGHGAPSSWVAIDSGKWARAVGFDKLTYCGVEC
                     GAPKSEIKETTSAAYLMSEFHFDHWLPVPVRGDLGVRYVRTDQLAVGYIPVKAPAGST
                     YPTVGQRNEVKRDYDDVLPSMNLVFDLKPELLLRVSAAKVMARPDLSALTPSSGVNGV
                     TRTGSINNPYLDPIRANTFDASLEWYFKPGSLASVGYFYKDISSYIQKINEQVPFNQL
                     GLPDSLLEGTNTAPTEIFSVSRLKNTPGGPLKGVELNLQTDFDFLPGAWKNVGVLANY
                     THVTSQIEYILTSNNGAPTSTTKNDLVGLSRDSASGTLYYEDKKVSVRATGTWRGSYI
                     RGIPASTGSDLQGNAPTFYLDASASYNLSPRLKLILDAQNLTDEMNRLYIDSTRQDTL
                     FISRVGRTFSLGLTYKY"
BASE COUNT          386 a          667 c          704 g          400 t
ORIGIN      
        1 cggcgagacg acgaaggccg actatcagga cgtcttcatc ccggcggtcg acgcggcgct
       61 gaaaacgcat gacaaggtcg cgctctatta tgagacggcg cccgacttca ccggctacag
      121 ccccggcgcc atgggggagg acgccgttct gggctttcgc cactatttcc agttttcccg
      181 cgtcgcgatt ctcagcgacg tcggctggat tcgcggcgcc gtcgccgcgt ttcgcccgct
      241 ctttccctgc cccgtgcggc tctatcgccc ggacgaggcg gcggcggccc ggcgctggat
      301 cgaaaccggt caggattaaa accgcataag cggacgctag ccttaaataa aaaaaggccg
      361 cccgctttat gcggacggcc gggcagttgg acgggaggaa gcgtcagtac ttgtaggtca
      421 gccccaggct gaaggtccgg ccgacgcgcg agatgaacag ggtgtcctgc cgcgtggagt
      481 cgatatagag ccggttcatc tcgtcggtca ggttctgcgc gtcgaggatc agcttcaggc
      541 gcgggctgag attgtacgag gccgaggcgt cgagatagaa ggtcggcgca ttgccctgca
      601 aatcgctgcc cgtgctggcc gggatgccgc ggatatagct gccgcgccat gtgccggtgg
      661 cgcgcaccga caccttcttg tcctcatagt acaacgtgcc gctggccgaa tcgcgcgaca
      721 ggccgacgag atcgttcttc gtcgtcgagg tcggcgcgcc gttattgctg gtcaggatgt
      781 actcgatctg cgaggtgacg tgcgtgtaat tggccagcac gccgacattc ttccacgcgc
      841 cgggcaggaa gtcgaaatcg gtttgcaggt tcagctccac gcccttgagc ggcccgcccg
      901 gcgtgttctt caggcggctg accgaaaaga tctcggtcgg cgcggtgttg gtgccttcca
      961 gcagcgagtc gggcaggccc agttgattga acggaacctg ctcgttgatc ttctggatat
     1021 aggacgagat gtccttgtag aaatagccga ccgaggccag agaccccggc ttgaaatacc
     1081 attccagcga ggcgtcgaag gtgttggcgc ggatcggatc gagatacgga ttgttgatcg
     1141 agccggtgcg cgtcaccccg ttgacgcccg acgacggcgt cagggccgac aggtcgggcc
     1201 gcgccatcac cttggccgcc gacacgcgca gcagcagttc cggctttaga tcgaagacga
     1261 ggttcatcga tggcagcacg tcgtcgtaat cgcgcttgac ctcgttgcgc tggccgaccg
     1321 tcggataggt cgaaccggca ggcgccttga ccgggatgta gccgaccgcc aactggtccg
     1381 tcctgacgta gcgcacgccc agatcgccgc gcaccggcac gggcagccaa tgatcgaaat
     1441 ggaattccga catcagatag gccgcgctgg tcgtctcctt gatctcggac ttgggcgcgc
     1501 cgcattcgac gccgcaatag gtcagcttgt cgaagcccac cgccctggcc cacttgccgg
     1561 aatcgatggc gacccagctc gacggcgcgc cgtggcccca caggtcgtcc atgccggtga
     1621 tggtggtggt aatgccggcc agatcgacgc ccgactgcgt cgccgcctga ccgggcgtca
     1681 ggctgagcgc gtgcgagttg aagtcctcca cgcgcccctc ggcgcccagc ttgacggcga
     1741 tagtgtcgtt ggccgtgtat ttcacctcga aggtcgcggt cttgttggtg gtgtcgttga
     1801 acaggggccg cccctgcaac gagaagccgc ccagcacgtc gcccgccgca ttggccggcg
     1861 catagttgaa attggcgacg ttggcgacgt cgatcccgtc gaacttgatc gtcggtacgc
     1921 cgcgattgtc gccgccgaaa tcgagcgaga aatcgtcggt gtcgatggcg tccatatagg
     1981 tctgcaaacg cagcggactg gtatatttcg agtcggacga accgatcatc gccgagatcg
     2041 cccaccggtc cgagaagcgg tgcgtcaggt tcagattgac ctgaccgaag gtggtcgaaa
     2101 accggtcgac cagcccttcg gagcgcacgt ccaccccgtc gaacaggccg tagagca
//
LOCUS       DASVLL010000067         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_272807,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000067 DASVLL010000000
VERSION     DASVLL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272807"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VF402_01175"
     CDS             <1..1003
                     /locus_tag="VF402_01175"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX7798928.1"
                     /translation="FAPADGVKNGWQAVFRSRAIDGPYAARDVMDQGPPDDLGPANGP
                     HQGALIDAPDGKSWFLHFQDTDSYGRRVWLEPVTWKDGWPVIGREGENGIGRPVAGGE
                     KPVQGQGVSVPVAGDDFAHGLNLGWQWNANPGADWADAATPGELRLKAVPSSANLWEA
                     GNVLTRKLPGMAFSVTTKLTLDARNVGERAGLVLMGRAYGFIGLVATKDGTRVEQVVR
                     DSAKNGDPETQVLAEAPVTGPVWLRMTLAPYVHPTGPSEYRPQWPAMLSLTDARVRFG
                     YSLDGVTFAPLGDGFVSQPGEWVGAQIGLFAQAPTGTPGASAVRNGVATFGPVRVTP"
     gene            complement(1010..>1601)
                     /locus_tag="VF402_01180"
     CDS             complement(1010..>1601)
                     /locus_tag="VF402_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7798929.1"
                     /translation="VPAVHSIVFTGLLPLSTAGWATLLGEKRPSAPFWLFSGLGAACI
                     LGFALHEGVSASPADVLLALAVIVCGLGYAVGARLGRRLGGWQVICWALLLALPVMAP
                     LMLATLPPHPAQVPPGAWLGLGYVTLFSMLIGFFFWYRGLALGDIAALGQLQLLQPFL
                     GLILAAVTLKETVSHAALIVMTLIVACVLGARRFSA"
BASE COUNT          279 a          531 c          601 g          190 t
ORIGIN      
        1 cttcgcgccc gccgacgggg tcaagaacgg ctggcaggcg gtgttccggt cgcgcgccat
       61 cgacggcccc tacgcggcgc gcgacgtgat ggatcagggg ccgccggacg atcttggtcc
      121 cgccaacggg ccgcatcagg gcgcgctgat cgacgcgccg gacggcaaaa gctggttcct
      181 gcattttcag gacacggaca gttacggccg ccgcgtctgg ctggagccgg tgacgtggaa
      241 ggacggctgg ccggtgatcg ggcgcgaagg cgaaaacggc ataggccgtc cggtggcggg
      301 cggcgaaaag ccggttcagg gccaaggcgt gtcggtcccg gtcgcgggcg acgatttcgc
      361 gcacgggctg aacctcggct ggcagtggaa cgccaatccg ggcgcggact gggcggacgc
      421 ggcgacgccg ggcgagctgc ggctgaaggc ggttccgtcc tcggccaatc tgtgggaggc
      481 gggcaatgtg ctgacgcgga aactgcccgg catggcgttc agcgtgacga cgaagctgac
      541 tctggacgcg cgcaatgtcg gcgagcgcgc ggggctggtc ctgatgggcc gcgcctatgg
      601 ctttatcggg ctggtcgcga ccaaggacgg aacgcgcgtc gagcaggtgg tgcgcgatag
      661 cgcgaaaaac ggcgatcccg agacgcaggt tctggccgag gcgccggtca cggggccggt
      721 gtggctgagg atgacgctgg cgccctatgt ccacccgacc ggcccgtccg aatacaggcc
      781 gcaatggccc gccatgctaa gcctgaccga cgccagggtt cgcttcggct acagcctcga
      841 cggcgtgacg ttcgcgccgc tgggcgacgg cttcgtcagc cagcccggcg aatgggtcgg
      901 cgcgcagatc ggcctgttcg cgcaggcgcc gaccggcacg ccgggcgctt cggcggtgcg
      961 caacggcgtg gcgacgttcg ggccggtgcg ggtgacgccc tgacgcggct caggccgaaa
     1021 agcgccgcgc gccgaggacg cacgccacga tcagcgtcat gacgatcagc gcggcgtggc
     1081 tgaccgtctc tttcagcgtc acggcggcga ggatcaggcc gaggaagggc tgcaacaact
     1141 gcaactgccc cagcgcggcg atgtcgccga gcgccagccc gcgataccag aagaaaaacc
     1201 cgatcagcat ggagaaaagc gtcacatagc ccagtcccag ccacgcgccg ggcggaacct
     1261 gcgccggatg cggcggcagg gtcgccagca tcagcggcgc catgaccggc agggccagca
     1321 gcagcgccca gcagatgacc tgccagccgc cgaggcgccg ccccagccgc gcgccgacgg
     1381 cgtagccgag gccgcagacg atcaccgcca gagccagcag gacgtcggcg ggcgaggcgc
     1441 tgacgccttc atgcagcgcg aagccgagaa tgcaggccgc ccccagaccg gagaacagcc
     1501 agaagggcgc ggacgggcgc ttttcgccca gcagcgtggc ccagcccgcc gtcgacagcg
     1561 gcaaaaggcc ggtgaagacg atggaatgca ccgccggaac c
//
LOCUS       DASVLL010000068         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_110936,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000068 DASVLL010000000
VERSION     DASVLL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110936"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF402_01185"
     CDS             <1..491
                     /locus_tag="VF402_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 36"
                     /protein_id="HEX7798930.1"
                     /translation="ENGSVYNHAAIFYIYALYQVRESEKAFDLLCKILSGPETADYLR
                     RGQLPIFVPNYYRGAHRQFPQASGRSSHLFNTGTAAWLYRTVIEGLYGLKGGREGLWV
                     RPQLPGEWLSARVTRRFRGATFHIDIRRGPPALRVDGVEQADACIRGVEAGRTYAVEV
                     SL"
     gene            complement(486..1772)
                     /locus_tag="VF402_01190"
     CDS             complement(486..1772)
                     /locus_tag="VF402_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX7798931.1"
                     /translation="MNLHYGPENLQALLAIAVILSLCWGLSENRGRFPFGLAAGAIAV
                     QVALILILFAIPQSHALLNGIGAGMNALSAATSQGMQFVFGYMGGGDQPYQVVNSGAL
                     FVFALQVLPLILVIAALSALLWHIGVLKWIIRGFGFVFEKLIGLGGASALGVASNIFV
                     GNVESLIIIRGYLDKLTRSEVFMMMTVGMANVSGSTMVAYVLILNHVLPNAAGHVLAA
                     AIVSAPAGVLLARIMVPELADRKAEKIDYSSSLRYDSAIDAVSKGTQDGMMVATNIAA
                     ILIVMVALVALFNMILGVFPHVGGQPLTIERILGTIFTPLAWLIGIPWKEAATGGGIL
                     GVKMVLTEFVAFLRLATIPVADMTERTRIILTYATCGFANIGSVGISVAGFSALMPSR
                     RKEILGLVWKALLSGFLATCLSAAIVGALPNAVYGL"
BASE COUNT          362 a          622 c          577 g          293 t
ORIGIN      
        1 ccgagaacgg ctcggtctat aaccacgccg ccatcttcta tatctacgcg ctgtatcagg
       61 tgagggaatc cgaaaaagcc tttgatctgc tatgtaaaat cttgagcgga cctgagacgg
      121 cggattatct gcggcgcggg cagctgccga tcttcgtccc caactattac cgcggcgcgc
      181 accgccagtt tccgcaggcg tcgggccgtt cgtcgcacct gttcaacacc ggcacggctg
      241 cgtggctcta tcgcacggtg atcgaggggc tgtacggcct gaagggcggc cgcgaaggtt
      301 tgtgggtgcg cccgcaactg cccggagaat ggctgtcggc gcgcgtgacg cggcgctttc
      361 gcggcgcgac cttccacatc gacatccggc gcggcccgcc cgccctgcgc gtcgacggcg
      421 tcgaacaggc cgacgcctgc atccgcggcg tcgaggcggg ccggacctat gcggtcgagg
      481 tcagcctata gcccgtacac cgcgttcggc agggcgccga cgatggcggc ggacaggcag
      541 gtggccagga agcccgacag cagcgccttc cagacgaggc cgagtatctc cttgcgccgc
      601 gacggcatca gcgccgagaa accggcgacc gaaatgccga ccgagccgat attggcgaag
      661 ccgcaggtcg cgtaggtcag gatgatgcgc gtgcgctcgg tcatgtcggc gaccgggatg
      721 gtggccagcc tgaggaaggc gacgaactcg gtcagcacca tcttcacgcc cagaattccg
      781 ccgcccgtcg ccgcctcctt ccacggaatc ccgatcagcc aggccagcgg cgtgaagatg
      841 gtccccaaaa tgcgctcgat ggtcagcggc tggccgccga cgtgcgggaa gacgccgagg
      901 atcatgttga acagcgcgac cagcgccacc atcacgatca ggatggcggc gatattggtc
      961 gccaccatca tgccgtcctg cgtgcccttg gacacggcgt cgatggcgct gtcgtatctg
     1021 agcgacgacg aatagtcgat cttctccgcc ttgcggtcgg ccagctccgg caccatgatc
     1081 cgcgccagca gcacccccgc cggcgccgag acgatggcgg cggccaggac atgcccggcg
     1141 gcgttgggca gcacgtggtt gaggatcagc acataggcca ccatggtcga tcccgacacg
     1201 ttggccatgc cgaccgtcat catcatgaac acctcggagc gcgtcagctt gtcgagatag
     1261 ccgcgaatga tgatcaggct ctcgacattg ccgacgaaga tgttggacgc cacgcccagc
     1321 gccgaggcgc cgcccagccc gatcagcttc tcgaacacga agccgaagcc gcggatgatc
     1381 catttcagca cgccgatatg ccacagaagc gccgacagcg cggcgatgac caggatcagc
     1441 ggcagcacct gcaaggcgaa cacgaacagc gcgccggaat tgaccacctg atagggctgg
     1501 tcgccgccgc ccatgtagcc gaatacaaac tgcatcccct gactggtcgc cgccgacagg
     1561 gcgttcatgc ccgcgccgat gccgttcagc aaggcgtgcg actgcgggat ggcgaacagg
     1621 atcaggatca gcgccacctg caccgcaatc gcgcccgccg ccagcccgaa cggaaaccgc
     1681 ccgcggttct ccgacaggcc ccagcacagg ctcagaatga ccgctatggc caacagggcc
     1741 tgaagattct ccggtccgta gtgcaaattc atgtacgctt ttcccgaaac agccagacgc
     1801 gcgttatcca atatcatttg aaaccttggc aaggtttatt ttgccccgcc ccga
//
LOCUS       DASVLL010000069         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_487327,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000069 DASVLL010000000
VERSION     DASVLL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487327"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2368
                     /locus_tag="VF402_01195"
     CDS             <1..>2368
                     /locus_tag="VF402_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase/phage tail family protein"
                     /protein_id="HEX7798932.1"
                     /translation="VFRRPRGDVVDMESRIEGVCLIPGAGEFIYATAPNALRTGLTGA
                     AFETQHTGGSATDFIVSLDQLQAQLPQVRRVNLVVSWFGDSLAAGSCTIRPGVESADR
                     VTAPLSWSVAGLSRDEAHVVTHVAGRPAYGGTPSDQVVIDAIAALKARGFEVTLIPFI
                     LMDCDGFPWRGRITSANDRTAAAADDAAHFLGGPDDWGLRRFVLHVASLGVAAGGVGA
                     IVLGSELRGLTWLRDDAGGYPMVAGLRALAADVRAVVGAGTKIGYGADWSEYFGHQPG
                     DGTGHAVFHLDPLWADGNIDFVGVDWYAPLTDWRDGTEHLDAALASSIYDDAYLMSRI
                     RGGEGFDWYYASAGNRDAQVRTPIHDGAYGEDWMFRPKDIVGWWSNPHHDRPGGVRAA
                     AATAWVPQSKPVRFIEIGCAAIDKGPNAPNLFLDPKSSESAVPPYSSGERDDRAQRAF
                     LGAYHEWYAANNPVSPVYGGPMLDGMAVWCWDARPYPAFPQRGDVWGDTDNWRTGHWL
                     NGRVGAGDARNLIAEIAGQAGIAPEQLDLSEVTGGVEGYAFAPPLSAKAALAPVLAWL
                     GLEAAPRGLGLGLVGAAHGVDLAVTRDQLAYRDASPLKVRRDLVAVPGSLTLRCYDPD
                     RDYQTQAVTVRADGRTGAGRTEIDLPLALSEAQAQAYAAYLLQSAQGVRHTLTINADP
                     LLMMTLQIGDGVMLEGDATVYRVTGLDAGETPNVTLTPSPSTRAVVATAPCGGSAMEG
                     GGLSLITGFTLLELPCFGLDESNVLPILVPAASPWLGCDVYAGAGATSL"
BASE COUNT          322 a          743 c          927 g          376 t
ORIGIN      
        1 ggttttccgc cgcccgcgcg gcgacgtcgt cgatatggaa agccggatcg aaggcgtgtg
       61 cctgatcccc ggcgcgggcg aattcatcta cgccacggcg cccaatgcgc tgcgcacggg
      121 cctgaccggg gcggcgttcg agacgcagca caccggcggg tcggcgaccg atttcatcgt
      181 ttcgctcgac cagttgcagg cgcagttgcc gcaggtgagg cgcgtcaatc tggtggtgag
      241 ctggttcggc gacagcctcg cggcgggaag ctgtacgatc aggcccggcg tcgagagcgc
      301 cgaccgcgtc accgcgccgc tgagctggtc ggtggcgggt ctgagccggg acgaggcgca
      361 tgtggtcacg catgtggcgg gccgtccggc ctatggcggc acgccgtccg atcaggtggt
      421 gatcgacgcg atcgcggcct tgaaggcgcg cggcttcgag gtgacgctga tcccgttcat
      481 cctgatggat tgcgacggct ttccgtggcg cgggcggatc acgtcggcga acgaccggac
      541 ggcggccgcc gcggacgatg cggcgcattt tctcggcggg ccggacgatt gggggctgcg
      601 gcggtttgtg ctgcatgtcg cgtccctggg cgtcgcggcg ggcggggtcg gggcgatcgt
      661 gctggggtcg gaactgcgcg gcctgacctg gctgcgcgac gatgcgggcg gctatccgat
      721 ggtggcgggg ctgcgcgccc tggccgccga tgtgcgcgcc gtggtcggcg ccgggacgaa
      781 gatcggctat ggcgccgact ggagcgagta tttcggccat cagccgggcg acgggacggg
      841 ccatgcggtg tttcacctcg atccgttgtg ggcggacggc aatatcgact tcgtgggcgt
      901 cgactggtac gcgccgctga ccgactggcg cgacgggacg gagcatctcg acgccgcgct
      961 ggcttcgtcc atctatgacg acgcctatct gatgagccgg attcgcggcg gcgagggctt
     1021 cgactggtat tacgccagcg cggggaaccg cgacgcgcag gtccggacgc cgatccatga
     1081 cggcgcctat ggcgaggact ggatgttccg gcccaaggac atcgtcggct ggtggtcgaa
     1141 cccgcaccac gaccgtccgg gcggcgtgcg cgcggcggcg gcgacggcgt gggttccgca
     1201 gtccaagccg gtgcgcttca tcgagatcgg ctgcgcggcc atcgacaagg ggccgaacgc
     1261 gcccaacctg ttcctggacc cgaagagttc cgagagcgcg gtcccgccct attcgagcgg
     1321 cgaacgcgac gaccgggcgc agcgcgcgtt cctgggcgcc tatcacgaat ggtatgcggc
     1381 caataatccg gtcagcccgg tctatggcgg cccgatgctg gacggcatgg cggtgtggtg
     1441 ctgggacgcg cggccctatc cggcctttcc gcaacgcggc gacgtgtggg gcgatacgga
     1501 caactggcgc accgggcact ggctgaacgg gcgcgtcggg gcgggcgacg cgcgcaacct
     1561 gatcgccgaa atcgcgggtc aggcggggat tgcgccggag caactggacc tgtccgaggt
     1621 gacgggcggg gtcgaaggct atgcgttcgc cccgccgctg agcgcgaagg cggcgctggc
     1681 gccggtgctg gcctggctgg ggctggaggc ggcgccgcgc gggctgggtc tgggccttgt
     1741 cggcgcggcg catggcgtcg atctggccgt gacgcgcgat caactggcgt accgcgacgc
     1801 ctcgccgctg aaggtccggc gcgatctggt cgcggttccg ggcagcctga ccttgcgctg
     1861 ctacgatccc gaccgcgact atcagacgca ggcggtgacg gtgcgcgcgg acggccggac
     1921 gggcgccggc cggacggaga tcgatctgcc gctggcgctg agcgaggcgc aggcgcaggc
     1981 ctatgcggcc tatcttctgc aaagcgcgca gggcgtgcgg catacgctga ccatcaacgc
     2041 cgatccgctg ctgatgatga cgcttcagat cggcgacggg gtgatgctgg aaggcgatgc
     2101 gacggtctat cgcgtgaccg ggctggacgc cggcgaaacg ccgaacgtaa ccctgacgcc
     2161 gtcgccttcg acgcgcgcgg tcgtggcgac ggcgccgtgc ggcggcagcg caatggaggg
     2221 cgggggcctg agcctgatca ccggcttcac cttgctggag ctgccgtgtt tcgggctgga
     2281 cgaaagcaat gtcctgccga ttctggttcc ggcggccagt ccgtggctgg ggtgcgacgt
     2341 ctatgccggg gcgggggcga cgtccctg
//
LOCUS       DASVLL010000070         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_57345, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000070 DASVLL010000000
VERSION     DASVLL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57345"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VF402_01200"
     CDS             complement(<1..848)
                     /locus_tag="VF402_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7798933.1"
                     /translation="MTAHTINSTHERPGGGWNGGLVLLVLLALLAFCAAAAALDAPLL
                     ATPLGLLALFTLPGFYTVQPNQSVTLIVFGNYKGSDRATGLRWVPFWYGRKKVCLRVR
                     NVTSEALKVNDQRGNPIEIAANVVWRISDAAQALFDVDDYAAFVTIQIETALRETARQ
                     YAYDHGDDGQPTLRDDAEIVGQRLKADLQARVDVAGVAVDEAHLMHLAYAPEIAGSML
                     KRQQAEAVIAARQKIVAGAVGMVEQALEQLSARGVVRLDEERKAAMVSNLLVVLCADR
                     EAQPVV"
     gene            complement(906..1814)
                     /locus_tag="VF402_01205"
     CDS             complement(906..1814)
                     /locus_tag="VF402_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7798934.1"
                     /translation="MSEVGAPTLDQLQVLLCVHETGSFAAAARRMGRATSAISYAIDQ
                     LEFLLGVSLFDRVGTRKPQLTDAGRAVLAEARVVGHGVDVLRARVRSLKQGLEAEVSV
                     AVDVMLPTARLVDALQAFQTAFPAIPLRLHVEALGGITQLVLEKAAVVGVSGPLDLNV
                     EGLDRRHLGGVRLIPVAAPFHPLARAGKHAPGEARQHVQLVLTDRSPLTEGRDFGVIG
                     MKSWRLADLGSKHALLLAGVGWGAMPEFMVRGDLDAGRLVRLDLPDWRDAHYDFNAIW
                     RTDAPPGPAAQWLIERFAMQVGAQVA"
     gene            1903..2766
                     /locus_tag="VF402_01210"
     CDS             1903..2766
                     /locus_tag="VF402_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7798935.1"
                     /translation="MSTLTTARPRAIASTHQAFHTLEGDGFEVARAIPADAYEAIGPF
                     IFLDQFGPIEVAPGEAKGASAHPHAGIETLTLLLEGRSIHKDSLGNESVMQPGEVQWM
                     RAGRGVIHDEGPDDELRAKGGRNHGVQLWLNLPRGHKHDAPMYRHVKASEIPHLQGDK
                     AFARLVAGRSGGAQGPLTSFGDPFVAHVSLQAGGQIEIASDAAELALYVMTGNVAVGG
                     QSVRNGWLARLTPGGAVALSADAPAEALLIGGDPLDAPILRHGPFVMNSIGELEQAVR
                     DYYAGRMGRIL"
     gene            2804..3403
                     /gene="wrbA"
                     /locus_tag="VF402_01215"
     CDS             2804..3403
                     /gene="wrbA"
                     /locus_tag="VF402_01215"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX7798936.1"
                     /translation="MAKVLVLYYSSYGHIEAMAKAVAEGAREAGAEVDIKRVPETAPL
                     EVARAAHFKLDQEAPVATVADLENYDAIIVGVGTRFGRLASQMAAFWDQAGGLWAKGA
                     LHGKVGGAFTSTATQHGGQETTLFSIITSLLHFGMVVVGLDYGHAGQMTLDEITGGSP
                     YGASTITGGDGSRLPSENELTGARYQGRKVAQTAIKLHG"
     gene            3574..>3837
                     /locus_tag="VF402_01220"
     CDS             3574..>3837
                     /locus_tag="VF402_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798937.1"
                     /translation="MPKQITQLAALGFCLCLVACGGGSGGGSGSSSYSSGASSSSTSS
                     SAASSSASVSSPPDWTMFQGDASHTGYVDATFDAANFTKAWDWT"
BASE COUNT          668 a         1360 c         1201 g          608 t
ORIGIN      
        1 ttgacgaccg gctgggcttc gcggtcggcg cacagtacga ccagcaggtt cgacaccatc
       61 gccgccttgc gctcctcgtc cagccggacc acgccgcgcg ccgacaattg ctccagcgcc
      121 tgctcgacca tgccgaccgc gcccgcgacg atcttctgac gcgccgcgat caccgcctcg
      181 gcctgctggc gcttgagcat cgaaccggcg atttccggcg cgtaggccag gtgcatcagg
      241 tgcgcctcgt ccaccgccac gcccgcgacg tcgacgcgcg cctgcaagtc ggccttcagc
      301 ctttggccga cgatctcggc gtcgtcgcgc agggtcggct ggccgtcgtc gccgtgatcg
      361 taggcgtact ggcgcgcggt ttcgcgcagg gccgtctcga tctggatggt cacgaaggcg
      421 gcgtaatcgt cgacgtcgaa cagggcctgc gccgcgtccg agatgcgcca gacgacattg
      481 gcggcgatct cgatcgggtt gccgcgctgg tcgttgacct tgagcgcctc gctggtgacg
      541 ttgcgcaccc gtaagcagac ctttttacgc ccgtaccaga acggaaccca gcgcagcccc
      601 gtcgcgcggt cgctgccttt gtaattgccg aacacgatca gcgtcacgga ctgattgggc
      661 tgcaccgtgt aaaagcccgg cagggtgaaa agggccagca ggcccagagg cgtcgccagc
      721 agcggcgcat ccagggccgc cgccgccgcg cagaacgcca gaagcgccag caggaccagc
      781 aaaaccagcc ccccgttcca gcccccgccc ggacgctcat gtgtgctgtt gatcgtgtgc
      841 gctgtcatat cagcctccct taaccgccga aagtgatatc actttgatgt caggtcggca
      901 agcggttatg cgacctgcgc cccgacctgc atcgcgaagc gctcgatcag ccattgcgcg
      961 gcgggaccgg gcggcgcgtc ggtgcgccag atggcgttga aatcgtaatg cgcgtcccgc
     1021 cagtcgggca ggtccagccg caccagccgc ccggcgtcga gatcgccgcg caccatgaat
     1081 tccggcatgg cgccccagcc gaccccggcc agcagcagcg cgtgtttcga gcccagatcg
     1141 gccagccgcc agcttttcat gccgatcacg ccgaaatcgc gcccttcggt cagcggcgag
     1201 cggtcggtca gcaccagttg cacatgctgg cgcgcctcgc ccggcgcgtg tttgccggcg
     1261 cgcgccagcg gatgaaacgg cgccgccacc gggatcagcc gcaccccgcc cagatggcgg
     1321 cggtccagcc cctccacatt gagatcgagc ggccccgaca cgccgaccac cgccgccttt
     1381 tccagcacca actgggtgat gccgcccagc gcctcgacat gcagccgcag cggaatcgcc
     1441 ggaaacgccg tctggaacgc ctgaagcgcg tcgaccagcc gcgccgtcgg cagcatgacg
     1501 tcgaccgcca ccgacacctc ggcctcaagg ccctgcttca ggctgcgcac ccgcgcgcgc
     1561 agcacgtcga cgccgtggcc gaccacgcgc gcctcggcca gcacggcgcg gcccgcatcg
     1621 gtcagttgcg gctttcgcgt tcccacgcgg tcgaacaggc tgacgccgag caggaactcc
     1681 agttggtcga tggcgtagct gatggccgag gtcgcccggc ccatgcggcg cgcggcggcg
     1741 gcgaaactgc ccgtctcatg cacgcacaat aagacctgca attgatccag cgtcggcgcg
     1801 cccacttctg acatatcgaa tttcctgaaa tgtttgttca atattatacg gattttccga
     1861 aaacgccagc gcgcctataa ggacggccaa ggagacataa gcatgtccac actgacaacc
     1921 gccaggcccc gcgccatcgc ctcgacccat caggcgtttc acacgctgga aggcgacggc
     1981 ttcgaggtcg cgcgcgccat tcccgccgac gcctacgagg cgatcggccc gttcatcttc
     2041 ctcgaccagt tcggccccat cgaggtcgcg ccgggcgaag ccaagggcgc ctcggcgcac
     2101 ccgcacgcgg gcatcgagac cctgaccctg ctgctggaag gccgctcgat ccataaggac
     2161 agcctcggca acgaaagcgt catgcaaccg ggcgaagtgc aatggatgcg cgccggtcgc
     2221 ggcgtcatcc acgacgaagg cccggacgac gaactgcgcg ccaaaggcgg tcgcaatcac
     2281 ggcgtccagc tctggctgaa cctgcccagg ggccacaagc acgacgcgcc gatgtaccgc
     2341 cacgtcaagg cgtccgagat tccgcacctg caaggcgaca aggcgttcgc gcgcctcgtc
     2401 gccgggcgct ccggcggcgc gcagggcccg ctgaccagct tcggcgaccc gttcgtcgcg
     2461 cacgtctcgc tgcaagccgg cggacagatc gagatcgcga gcgacgccgc cgaactggcg
     2521 ctttacgtca tgaccggtaa cgtcgccgtc ggcggccaaa gcgtgcggaa cggctggctg
     2581 gcgcggctga cgcccggcgg cgccgtcgcg ctcagcgccg acgcgcccgc cgaggcgctg
     2641 ctgatcggcg gcgatccgct cgacgcgccg atcctgcgcc acggcccgtt cgtgatgaac
     2701 agcatcggcg aactggagca ggcggtgcgc gactactatg cgggccgcat gggccgcatc
     2761 ctgtaatttc cctttttcca ccaagccttt aaggaatacg accatggcga aggttctcgt
     2821 tctctactac tcctcctacg gccacatcga ggcgatggcc aaggccgtcg ccgagggcgc
     2881 ccgcgaagcc ggcgccgagg tcgacatcaa gcgcgtgccc gaaaccgcgc cgctggaggt
     2941 cgccagggcc gcgcacttca agctggatca ggaagcgccg gtcgccacgg tcgcggacct
     3001 cgaaaactac gacgcgatca tcgtcggcgt cggcacgcgc ttcgggcgcc tcgcctcaca
     3061 gatggcggcc ttctgggatc aggcgggcgg cctgtgggcc aagggcgcgc tgcacggcaa
     3121 ggtcggcggc gccttcacct cgaccgcgac tcagcacggc gggcaggaaa ccaccctgtt
     3181 cagcatcatc acctcgctgc tgcacttcgg catggtggtg gtcggcctgg attacggcca
     3241 tgcgggccag atgacgctgg acgaaatcac cggcggctcg ccctacggcg ccagcaccat
     3301 caccggcggc gacggctcgc gcctgccgag cgagaacgaa ctgaccggcg cccgctatca
     3361 gggccgcaag gtcgcccaaa ccgccatcaa gctgcacgga taagcctctc tccccccggc
     3421 tccgtacaga ttgagcgccg ccgccgaacg cccgttcggc ggcggttttt attgcaattg
     3481 tcgtgatgtt ttctctttgc catattgcgc tgacaacggg taaaatcgta aattgcgaac
     3541 gttcgcctaa gggtgaaagc aggggggaaa ttcatgccga aacagattac gcaattggcc
     3601 gcgctcggct tttgtctttg tctcgtcgct tgtggcggcg gttcaggcgg cggctcaggc
     3661 agcagttcat acagttccgg cgcttcatcg tcttcaacga gttcgagcgc cgcatccagt
     3721 tccgccagcg tatcgtcacc ccctgactgg acaatgtttc aaggcgatgc ttcgcataca
     3781 ggctatgtgg atgcaacttt cgacgccgca aacttcacaa aagcctggga ctggacc
//
LOCUS       DASVLL010000071         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_273127,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000071 DASVLL010000000
VERSION     DASVLL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273127"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VF402_01225"
     CDS             <1..978
                     /locus_tag="VF402_01225"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7798938.1"
                     /translation="DDEGATPTLNSLLDRDFRYRQTLSAAYVTWQRELGAKWTVLGGL
                     RAEQLDLTTGQRTTGATAHIEDTHLNPSFYATYLLSRDAKLRFNYAHRLQRPTPEDYD
                     VSLNYGNGGWMRGNTHLKPQTTDAFEVNYEYAHESLSWSVRGFYQQNGDVITSSSRFI
                     ENPDGTGDPVLETTRGNDGSGDSGGVQLTWNNRVKDKLNLTLSATAAQDHVRAPAVPG
                     TQALTALSGSVNLSWTPTKKDSLSFRYQRAARSLTGEGYTGAFSQGSLNYSHRLGQLS
                     LTVDVGNLFMGKRESVTRTPIANSVAWTRLPGPVVSIGLRRSFKLTAGG"
     gene            complement(1035..1502)
                     /locus_tag="VF402_01230"
     CDS             complement(1035..1502)
                     /locus_tag="VF402_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7798939.1"
                     /translation="MSTCEDRLRALGLTLPAPTAPVANYVPFARTGNLVHISGQLSND
                     GKGGVKGTVGVDVSLEDAVEGARWCGLNLLAQMKAAAGDLDNVVRVIKLNAFVQAGPD
                     FYNIPQVVNGCSDLMVEALGEAGKHARSAVGMYKIPLGFAVEIDALIEVLDRR"
     gene            1729..>1808
                     /locus_tag="VF402_01235"
     CDS             1729..>1808
                     /locus_tag="VF402_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEX7798940.1"
                     /translation="MTQKKVLIVEDNDLNMKLFHDLLDAQG"
BASE COUNT          327 a          620 c          581 g          280 t
ORIGIN      
        1 gacgacgaag gcgcgacgcc gacgctcaac agcctgctcg atcgcgattt ccgctatcgt
       61 cagacgctca gcgccgccta tgtcacctgg cagcgcgaac tcggtgcgaa atggaccgtt
      121 ctgggcggct tacgcgccga acaactggac ctgaccaccg ggcagcgcac gaccggcgcg
      181 accgcgcata tcgaggacac gcatctcaac cccagcttct acgccaccta tcttctgtcg
      241 cgcgacgcca agctgcgctt caactatgcg caccgcctgc aacggccgac gccggaggat
      301 tacgacgtct cgctcaacta cggcaacggc ggctggatgc ggggcaatac gcatctgaag
      361 ccgcagacca ccgacgcgtt cgaggtcaat tacgagtacg cgcacgagtc gctgagctgg
      421 agcgtgcgcg gcttttacca gcagaacggc gacgtcatca cctcatcgag ccgctttatc
      481 gaaaacccgg acggcacggg cgatccggtg ctggagacga cgcgcggcaa tgacggttcg
      541 ggcgattccg gcggcgtgca gctcacctgg aacaaccgcg tcaaggacaa gctgaacctg
      601 accctgagcg cgaccgccgc gcaggaccat gtgcgcgcgc ccgccgtgcc gggaacgcag
      661 gcgctgacgg ccctgagcgg cagcgtcaac ctgtcgtgga cgccgacgaa aaaggattcc
      721 ctgtccttcc gctaccagcg cgcggcgcgc agcctgaccg gcgaaggcta tacgggcgcc
      781 ttttcccagg gcagtctgaa ctacagccac cgcctcggcc aactgagcct gaccgtggat
      841 gtcggcaacc tgtttatggg caagcgcgag agcgtgacgc gcacccccat cgccaacagc
      901 gtcgcgtgga cgcgcctgcc ggggccggtc gtctccatcg gcctgcggcg gtcgttcaag
      961 ctgacggcgg gcggataggc cggagcgcag gccccggcct cccgtcctga ctcagtcggg
     1021 gctcagccgg gggctcagcg ccggtccagc acctcgatca gggcgtcgat ctcgaccgcg
     1081 aagccgagcg gaatcttgta catgccgacc gccgagcgcg cgtgcttgcc cgcttcgccc
     1141 agcgcctcga ccatcaggtc ggagcagccg ttgacgacct gcgggatgtt gtagaagtcc
     1201 ggcccggcct gcacgaaggc gttgagcttg atcacgcgca ccacgttgtc gaggtcgccc
     1261 gccgccgcct tcatctgcgc cagcaggttc aggccgcacc agcgcgcgcc ttccaccgcg
     1321 tcctcaaggc tgacgtcgac gccgaccgtg cccttgacgc cgcccttgcc gtcgttcgac
     1381 aactggcccg aaatatggac gaggttgccc gtgcgcgcga acgggacgta attggccacc
     1441 ggcgcggtcg gcgcgggcag ggtgaggccg agcgcccgca ggcggtcttc gcaagtcgac
     1501 atgaggggtc tccagaaagg ttcgatgcgt cgcatataga cgcaagccgc aacccggcgc
     1561 cagcgccgtt tttacctttt gtttccttct gcttcacctc ggcttaagag agtccggcta
     1621 gattgccggg acgaacggag gcgggggcgc gcaggcgcga ttcgcctcgt cacgatgatg
     1681 gacggccgcc ggggcccatg cccgccgcgc ggccgaaggt aaggcttgat gacgcagaag
     1741 aaagtcctca tcgtcgagga caacgatctc aacatgaagc tgtttcacga cctgctcgac
     1801 gcgcaggg
//
LOCUS       DASVLL010000072         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_218400,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000072 DASVLL010000000
VERSION     DASVLL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218400"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..624)
                     /locus_tag="VF402_01240"
     CDS             complement(7..624)
                     /locus_tag="VF402_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798941.1"
                     /translation="MNATDVFINCPFSADYKERFWAIAYTVIRSGFEPRCALEADNSG
                     ENRYGKICRIIKECRYGVHDISYTGLYGEAAQDADFPLPRFNMPFELGLFLGAQSYGG
                     PKQTAKTTLILDTKPYRFQKFLSDIAGHDPRVYGGDATSLIEQVAAWLRVEGRRAEIP
                     GGEVIAADFAAFMAIFPDVCRAAKLNPDNITFFDFKRFAAAADLS"
     gene            complement(849..2105)
                     /locus_tag="VF402_01245"
     CDS             complement(849..2105)
                     /locus_tag="VF402_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX7798942.1"
                     /translation="MPNEPKPAFTAPRLLLFEYSELNPALREDPHAIFDPQRRERPVE
                     HDGMIPALLISSYGLGREALQATALSRNFNDAAPENPVIANVRRLSQAVEAEFGKPDS
                     ILTLDDPDHGRVRGVVAEAFLKRAAKAQGLVARVIDERLDALAARDGFDVVNDYAGQI
                     PIRVLGTLLGAAEDRLDDLKAWTEAGQLAFDPTKSAEQERLALDGRRGILGYFRELMA
                     QRRAEPRDDLVSDLLAAADRGAPIDDNEILHNLFALLTAGHLTTADLIGNGVHLLLSH
                     PEARRAVAETPKRIQAAVEEILRYEPPISFTARFAKDGGAIGGCPFHKGDALVVSLIS
                     ANRDEKRFDDPHRFDITRKPNHHMTFGAGAHICIGAPLARIEGQMAIGKLFERFPNLR
                     KADDAPPDWRVAPGVRGLARLDVKIA"
     gene            complement(2181..>4079)
                     /locus_tag="VF402_01250"
     CDS             complement(2181..>4079)
                     /locus_tag="VF402_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX7798943.1"
                     /translation="DQVRLIKALLEAENVDTKKWTPKSFAFHLDQWKNKGLTPDKVKN
                     DEGAAFANGRGVKFYQMYQDRLRILNACDFGDLLLHNLEILTRHPDVLERYHQRFKYI
                     LVDEYQDTNVAQYLWLRLLTNADRNLCCVGDDDQSIYGWRGAEVDNILKFERDYPGAK
                     VVRLERNYRSTQHILAAASGLIKANAARLGKTLFTDQTGGDKVRVLGLWDGAAEAQMV
                     AEDIERQKKAGRAWSDMAILVRASFQMRAFEERFVMLQIPYTVVGGPRFFERAEIRDA
                     LAYLRLIQSEDDDLAFERIVNVPKRGVGDATVQKLLQSARLAGVPVMRAVRDLVETDE
                     LPARAKTPLTGFLRDYDRWREQAKTLASDELLDRVLSDSGYYDMQRADKTSGQTRLDN
                     LKELGDSMQAFDNLEAFLEHVSLVMDLERDNGASSVRLMTLHAAKGLEFPVVYLPGWE
                     EGIFPSQRSMDENGVRALEEERRLAYVGLTRARQEARISFVANRQVYGRWTSQIPSRF
                     VDELPIEHVEASSQTGYQSGGGWASQESRWAKEHYGAGYEPNYKKRAPDNDLSGVDMS
                     RLRPKPAPHRPSAAPKPSAKNFRLGERVFHIKFGYGIVAEVEGNKLVVNFETSGDKKV
                     IDSFVERA"
BASE COUNT          693 a         1319 c         1286 g          781 t
ORIGIN      
        1 cctgtgtcaa gacaggtccg ccgcggctgc aaatcgtttg aaatcgaaaa aggtgatgtt
       61 gtcgggattc agcttcgccg cacggcaaac atccgggaaa atcgccataa aggcggcaaa
      121 gtctgcggca atgacctcgc cgccggggat ttcagcgcgg cggccctcga cccgcagcca
      181 cgccgccacc tgttcgatga gcgacgtcgc atcgccgcca tagactctcg gatcatgccc
      241 cgcgatgtcc gataaaaact tttggaaccg atacggtttg gtgtcgagaa ttaacgtcgt
      301 cttggcggtc tgtttcggcc cgccatagct ttgcgcgcca agaaaaagac ccagctcgaa
      361 aggcatgttg aaccgcggca aggggaaatc ggcgtcctga gcggcctcgc cgtacaagcc
      421 agtgtaggag atgtcgtgaa cgccgtagcg gcactccttt atgatccggc agattttacc
      481 gtagcggttt tcgccggaat tatcggcctc gagtgcgcat cgcggctcaa agcccgatct
      541 gatgacggtg taggcgatcg cccaaaaccg ttctttgtaa tctgccgaaa acggacagtt
      601 tatgaagacg tccgtcgcat tcatataggg tttttaatct cttgccgccg ccagattttg
      661 cgcacgcaca gcggcgacgg ctttgcgcgc ttcagccagc gtaatattat ccacgcggcc
      721 cttaggccgg aggattttga tgccttcctt cgtcaccccg atatagggac tccgtctgga
      781 aggcgcggct ttgagcttgg cgggcgcgct tttcatggac ctgaatatgg ccccgaaccg
      841 cttaaaaatc aagcgatctt cacgtccagc cgcgccagtc cgcgcacgcc cggcgccacg
      901 cgccagtcgg gcggggcgtc gtcggccttg cgcaggttcg ggaagcgttc gaacagcttg
      961 ccgatggcca tctggccttc gatgcgggcc agcggcgcgc cgatgcagat gtgcgcgccg
     1021 gcgccgaagg tcatgtggtg gttcggcttg cgcgtgatgt cgaagcggtg cggatcgtcg
     1081 aaccgctttt cgtcgcggtt ggccgagatc aggctgacca ccagcgcgtc gcccttgtgg
     1141 aacggacagc cgccgatagc gccgccgtcc ttggcgaagc gcgcggtgaa cgagatcggc
     1201 ggctcatagc gcaggatttc ctcgacggcg gcctggatgc gtttgggagt ctcggcgacg
     1261 gcccggcgcg cttcgggatg gctgagcagc agatgcacgc cgttgccgat caggtcggcg
     1321 gtggtcaggt gcccggcggt cagcagcgcg aacaggttgt gcaaaatctc gttgtcgtcg
     1381 atcggcgcgc ccctgtccgc ggccgccagc agatcgctga ccagatcgtc gcgcggttcg
     1441 gcccgccgct gcgccatcag ctcgcggaaa tagccgagaa tgccgcgccg tccgtccagc
     1501 gccagtcgct cctgctcggc ggacttggtc ggatcgaagg ccagttgccc ggcctcggtc
     1561 cacgccttga gatcgtcgag acggtcctcg gcggcgccca gcagcgtccc cagcacgcgg
     1621 atcggaatct gcccggcgta atcgttcacg acgtcgaagc cgtcgcgcgc ggccagcgcg
     1681 tccagccgtt cgtcgatgac gcgcgccacc agcccttgcg ccttcgccgc gcgtttcagg
     1741 aaggcttcgg ccaccacgcc gcgcacgcgg ccatgatcgg gatcgtccag cgtcaggatg
     1801 gaatcgggtt tgccgaactc ggcctcgacg gcctggctca ggcggcggac attggcgatc
     1861 accgggtttt ccggcgccgc gtcgttgaag ttacgcgaca gggccgtcgc ctgcaacgct
     1921 tcgcgcccaa ggccgtagga cgagatcagc agcgccggga tcatgccgtc gtgttcgacc
     1981 gggcgctcac ggcgctgcgg gtcgaaaatg gcgtgcgggt cttcgcgcag ggccgggttc
     2041 agctcggaat attcaaacaa aagcagtcgg ggtgcggtaa aggccggttt cggctcgttc
     2101 ggcatcagga ggtctccgat agttcgcccg cggatcaggt cggatgcaat acgcgggcca
     2161 tctcgccggt gcaatataat ctaggcgcgc tcaacgaagc tgtcgatgac ctttttgtca
     2221 ccgctggtct caaaattgac gacaagcttg ttgccctcca cctcggcgac gatcccgtaa
     2281 ccgaacttga tgtggaagac gcgctcgccc agcctgaagt ttttcgccga cggtttcggc
     2341 gccgccgagg gccggtgcgg cgccggtttg ggccgcagcc gcgacatatc cacgccggac
     2401 aggtcgttat ccggcgcgcg ttttttgtaa ttcggctcat acccagcgcc ataatgttct
     2461 ttggcccagc ggctttcctg cgacgcccag ccgccgccgc tctgatagcc ggtttgcgac
     2521 gacgcctcga catgctcgat cggcagttcg tcgacgaagc gtgacggaat ctggctggtc
     2581 cagcggccat agacctgacg gttggcgacg aagctgatgc gcgcctcctg ccgcgcgcgg
     2641 gtcaggccga cataggccag acggcgctcc tcctccagcg cgcgcacgcc gttttcgtcc
     2701 atcgagcgct gcgacgggaa aattccctcc tcccagccgg gcagatagac gaccggaaat
     2761 tccagcccct tggcggcgtg cagcgtcatc agccgcacgg aggacgcgcc gttgtcacgc
     2821 tcaaggtcca tgaccagcga aacgtgttcg aggaaggctt cgagattatc gaacgcctgc
     2881 atcgaatcgc ccagctcctt caggttgtcg agccgcgtct ggcccgacgt cttgtcggcg
     2941 cgctgcatgt cgtaatagcc gctgtcgctc agcacgcggt ccagcaattc gtccgaggcc
     3001 agcgtcttcg cctgctcgcg ccagcggtcg tagtcgcgca ggaacccggt cagcggcgtc
     3061 ttggcgcgtg cgggcaattc gtccgtctcg accagatcgc gcacggcgcg catcaccggc
     3121 acgcccgcca gccgcgccga ttgcaacagc ttctgcaccg tcgcgtcgcc gacgccgcgc
     3181 ttgggcacgt tgacgatgcg ctcgaaggcc agatcgtcgt cctcggactg gatcagccgc
     3241 agataggcca gcgcgtcgcg gatttcggcg cgctcgaaga agcgcggccc gccgaccacg
     3301 gtatagggga tttgcagcat gacgaagcgt tcctcgaacg cccgcatctg gaacgaggcg
     3361 cgcaccagga tggccatgtc ggaccacgcc cgcccggcct tcttctggcg ctcgatgtcc
     3421 tcggcgacca tctgcgcctc cgccgcgccg tcccacaggc ccagcacgcg caccttgtcg
     3481 ccgccggtct gatcggtgaa cagggtcttg cccagccgcg ccgcgttggc cttgatcagc
     3541 cccgacgcgg cggccagaat gtgctgcgtc gagcggtagt tgcgctccag ccgcaccacc
     3601 ttggcgcccg gatagtcgcg ctcgaacttg aggatgttgt cgacctcggc gccgcgccag
     3661 ccatagatcg actgatcgtc gtcgccgacg cagcagaggt tgcggtcggc gttggtcagc
     3721 agccgcagcc acagatactg cgcgacgttg gtgtcctgat attcgtcgac caggatgtat
     3781 ttgaaccgct gatggtaacg ctccagcacg tcgggatgcc tggtcagaat ctccagattg
     3841 tgcagcagca gatcgccgaa atcgcaggcg ttcaggatgc gcagccggtc ctgatacatc
     3901 tgatagaatt tgacgccgcg cccgttcgca aacgccgcgc cctcgtcgtt tttcaccttg
     3961 tccggcgtca gccccttgtt cttccactga tcgagatgga aggcgaagga tttgggcgtc
     4021 catttcttgg tatcgacgtt ttcggcttcg agcagcgcct tgatcagccg cacctgatc
//
LOCUS       DASVLL010000073         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_273231,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000073 DASVLL010000000
VERSION     DASVLL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273231"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VF402_01255"
     CDS             <1..1356
                     /locus_tag="VF402_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX7798944.1"
                     /translation="LEVFHGLLGLDEKELLKQTQGVYSLGQSFANFSGPRPPFFHPYG
                     DHGVALGASSFAQIWFKARQAGLKVDFEDFSPNAAAAKAGRFFVGGETARAFGRCNYA
                     YHLHGQAYVQALKALALRMGVKVTPARGFDAVLDPDSGLIRALALSDGRQVAGDFFID
                     ATGGESRLLGQALQGAFIDWSRWLPMNRTLSAGGDRLRVLPAYSQVRALEAGCLHMTA
                     LQTMTGVTHAYSGDWLSDEQALEALNVTAGIGLSGDATLSVRAPGRRERLWSRNCVAI
                     GEAGCVLDPIDNPDLHVLQIGLSHLIALFPLAVEGGVEAAQYNRLVGGAYERLRDYQI
                     AHYRLNRQNHPFWDALRDVAPPDSLAARLDLYKARGEILLYEDETFQNDDWLSLFIGH
                     GLMPDAWDPRIDMLPEDQLILHVQKLLGFIRDQVQAMDSHDAYIELFAARSFAGFGSQ
                     L"
     gene            1353..>1539
                     /locus_tag="VF402_01260"
     CDS             1353..>1539
                     /locus_tag="VF402_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX7798945.1"
                     /translation="MSEPLLRRIVIVGGGTAGWMAAAMLSKTLGTQNYDITLIESEEI
                     GTVGVGEATVPPINIFNE"
BASE COUNT          245 a          488 c          534 g          272 t
ORIGIN      
        1 ctggaggtct ttcacggcct gctcggcctc gatgagaaag agctgctcaa acagacgcag
       61 ggcgtctaca gcctcgggca gagctttgcg aacttttccg gtccgcgtcc gccgtttttc
      121 catccttacg gcgatcatgg cgtggcgctc ggcgcgtcgt cgttcgcgca gatctggttc
      181 aaggcgcgtc aggcgggcct gaaggtggat ttcgaggact tctcgcccaa cgcggcggcg
      241 gcgaaggcgg ggcgcttctt tgtcggcggc gagacggccc gcgcgttcgg gcgctgcaat
      301 tacgcctatc atctgcatgg ccaggcctat gtgcaggccc tgaaggcgct ggccctgcgc
      361 atgggcgtca aggtcacgcc ggcgcgcggc ttcgacgcgg tgctcgaccc ggacagcggc
      421 ctgatccgcg cgctggccct gagcgacgga cggcaggtcg cgggcgattt cttcatcgac
      481 gcgacgggcg gggaaagccg cctgctggga caggccctgc agggcgcgtt catcgactgg
      541 tcgcgctggc tgccgatgaa ccgcacgctg agcgcgggcg gcgaccgcct gcgcgtcctg
      601 cccgcctatt cgcaggtgcg cgcgcttgag gccgggtgcc tgcacatgac ggcgttgcag
      661 accatgacgg gcgtgaccca cgcctacagc ggcgactggc tgagcgacga acaggcgctg
      721 gaggcgctga acgtgacggc ggggatcggc ctgagcggcg acgcgacctt aagcgtgcgc
      781 gcgccgggcc gccgcgaacg cctgtggtcg cgcaactgcg tggcgatcgg cgaggccgga
      841 tgcgtgctcg atccgatcga caatcccgac ctgcatgtcc tgcagatcgg cctgtcgcat
      901 ctgatcgccc tgttcccgct ggcggtcgaa ggcggggtcg aggcggcgca gtacaaccgt
      961 ctggtcggcg gcgcctatga acgcctgcgc gactatcaga tcgcgcacta caggctgaac
     1021 cgccagaacc atccgttctg ggacgcgctg cgcgacgtcg ccccgccgga cagtctggcg
     1081 gccaggctcg acctctacaa ggcgcgcggc gaaatcctgc tgtatgagga cgagacgttc
     1141 cagaacgacg actggctgtc ccttttcatc ggtcacggcc tgatgcccga cgcgtgggat
     1201 ccgcgcatcg acatgctgcc ggaggatcag ctcatccttc atgtccagaa gctgctgggt
     1261 tttattcgcg atcaggttca ggcgatggat tcgcatgacg cctatatcga actgttcgcc
     1321 gcgcgcagct tcgccggttt cgggagtcaa ttatgagcga gcccctgttg cgcaggatcg
     1381 tgatcgtcgg cggcggcacg gcgggatgga tggccgcagc catgttgtcc aagacgctgg
     1441 gcacgcagaa ttatgacatc accctgatcg aatccgagga gatcggcacg gtcggcgtcg
     1501 gcgaggcgac ggtgccgccg atcaatatct tcaacgagg
//
LOCUS       DASVLL010000074         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_273239,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000074 DASVLL010000000
VERSION     DASVLL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273239"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VF402_01265"
     CDS             complement(<1..779)
                     /locus_tag="VF402_01265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7798946.1"
                     /translation="MGLDSNLPQTPAAAPATGAPQAARATTASMQSLLAQYFADHGPQ
                     GLSPVHESQAALAAALAPPEADAESGWPRAARDEVAGLRAEIAELRAQLERQTRLLQS
                     AQAQADHDVLTPTLNRRAFLRETQRAMADCKRYGEEATLIYIDMDGFKGVNDAYGHAA
                     GDAALIYVAETLNANVREGDSVGRMGGDEFAVLLRHADLKSARVKAMKLEAELSMGTF
                     EHNGLYLKTGASFGVRAWAGQASAESWLAEADAAMFLVKKSS"
     gene            990..1481
                     /gene="purE"
                     /locus_tag="VF402_01270"
     CDS             990..1481
                     /gene="purE"
                     /locus_tag="VF402_01270"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7798947.1"
                     /translation="MPQSPGVAIIMGSRSDWAVMKNAAAVLDQLGVAYTAQVISAHRT
                     PKRMVAFAEGARAAGYKVIIAGAGGAAHLPGMTASMTTLPVLGVPVPVRDGLKGIDSL
                     LSIVQMPGGVPVGTLAVGEAGAKNAGQLAAQILALGDPALAERLEAFRAAQTDGVPET
                     VED"
     gene            1481..2557
                     /locus_tag="VF402_01275"
     CDS             1481..2557
                     /locus_tag="VF402_01275"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX7798948.1"
                     /translation="MLDTPLSLGATIGVLGDGQLGRMMSQAAARLGFKVAVFGPEAGA
                     PAAQVSASATVAGYDDEAALRRFAAQCDVITLEFENIPAATLDVLTGLGKVVAPGAHA
                     LRVTQDRVLEKTFARDHGVATVDFRAVDAAGDLEAALAALGTPALLKTRRDGYDGKGQ
                     AWIRSPAEAGAAWNAVGGKPSILEAKASFTREISVVAARGWNGEIRAFPIGENHHVDG
                     ILATTLAPAPVSAAVTQAAHDIARRVLEGLDYVGVLAVELFVLDGDRLVLNEIAPRVH
                     NSGHWTQEGCVCDQFEQHVRAVAGWPLGDPSARCTVEMRNLLGDDILDWPRLAAEPNA
                     QLHVYGKAEPRPGRKMGHINRIKS"
     gene            2557..2841
                     /locus_tag="VF402_01280"
     CDS             2557..2841
                     /locus_tag="VF402_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7798949.1"
                     /translation="MFLLSLTYKVPLDVVDRHLDAHVAWLKEGYASGMFLASGRKIPR
                     TGGMIFAQGERAAVEAFARLDPFAVNGVADYAVTEVNLTMTAEGLEGLKG"
BASE COUNT          431 a          975 c         1062 g          462 t
ORIGIN      
        1 gacgacttct tgaccaggaa catggcggcg tcggcctcgg ccagccagct ttccgccgag
       61 gcctgacccg cccaggcgcg cacgccgaac gaggcgccgg tcttgagata gaggccgttg
      121 tgctcgaacg tgcccatgct cagctcggcc tccagcttca tcgccttgac gcgcgccgat
      181 ttcaggtcgg cgtggcgcag aagcacggcg aattcgtcgc cgcccatgcg tccgacgctg
      241 tcgccctcgc gcacattggc gttgagcgtc tcggcgacat agatcagcgc cgcgtcgccc
      301 gccgcgtggc cgtaggcgtc gttgacgccc ttgaagccgt ccatgtcgat atagatcagc
      361 gtcgcctcct cgccatagcg cttgcagtcg gccatggcgc gctgcgtctc gcgcaggaag
      421 gcgcggcggt tcagcgtcgg cgtcagcacg tcgtgatcgg cctgcgcctg cgcgctttgc
      481 aaaagccggg tctggcgctc gagctgcgcg cgcaattcgg cgatctcggc gcgcaggccc
      541 gcgacctcgt cgcgcgcggc gcgcggccag ccggattcgg cgtcggcttc gggcggcgcc
      601 agcgcggcgg ccagcgccgc ctggctttca tgcaccggcg acagcccctg cgggccgtga
      661 tcggcgaaat actgcgccag caggctctgc atcgaggcgg tggtggcgcg cgcggcctgc
      721 ggggcgccgg tagcgggcgc cgcggcgggc gtctggggga ggttggaatc gaggcccatc
      781 tgtccgggaa aatctgggga aagcgccgtc cgttctggcc gcgcgggtgg cctacagaaa
      841 agcatggacg ccgcaaaaaa acaatccggc gcttccgatt tggggatatt gccggggcat
      901 gaatgagcgc ttgcagtcgg ggacttcgcc gctatactcc gcgcctttcc gaaaggggct
      961 tatcgcccgc cgtcacaagg gatttcgcca tgccgcaatc gccaggcgtc gccatcatca
     1021 tgggctcgcg ctcggactgg gccgtgatga agaacgccgc cgccgtgctg gaccagctcg
     1081 gcgtggccta tacggcgcag gtgatctccg cgcaccgcac ccctaagcgc atggtcgcgt
     1141 tcgccgaggg cgccagggcg gcgggctaca aggtgatcat cgccggggcg ggcggcgccg
     1201 cgcatttgcc gggcatgacc gcgtcgatga ccaccctgcc ggtgctgggc gtgccggttc
     1261 cggtgcgcga tgggctgaaa ggcatcgatt cgctgctcag catcgtgcag atgccgggcg
     1321 gggtgccggt cggcacgctg gccgtcggcg aggcgggggc gaagaacgcc gggcaactgg
     1381 cggcgcagat tctggcgctg ggcgatccgg ctctggccga acggctggaa gccttccgcg
     1441 cggcgcagac cgatggcgtg cctgagacgg tggaggactg atgctggaca cgccgctttc
     1501 ccttggcgcg accatcggcg tattgggcga cgggcaactg ggccggatga tgagccaggc
     1561 cgccgcgcgc ctcggcttca aggtcgcggt gttcggcccg gaagcgggcg cgcccgccgc
     1621 gcaggtgtcg gcctcggcca cggtcgccgg ttatgacgac gaagccgcgc tgcggcgctt
     1681 cgccgcgcaa tgcgacgtca tcacgctgga gttcgagaac attcccgccg cgactctgga
     1741 cgtgctgacc ggcctcggca aggtggtcgc gcccggcgcg cacgccttgc gcgtcacgca
     1801 ggaccgcgtg ctggaaaaga ccttcgcgcg cgaccacggc gtggcgacgg tcgattttcg
     1861 cgccgtcgat gcggcgggcg atctcgaagc ggcgctggcg gcgctgggga cgcctgccct
     1921 gttgaaaacg cgccgcgacg gctatgacgg caaggggcag gcgtggattc gctcgcccgc
     1981 cgaggccggg gccgcatgga acgcggtcgg cggcaagccg tccattctgg aggcgaaggc
     2041 cagcttcacg cgcgagattt cggtggtggc ggcgcgcggc tggaacggcg agatccgcgc
     2101 ctttccgatc ggcgagaacc accatgtcga cggcatactg gcgacgacgc tggcgcccgc
     2161 gccggtcagt gcggcggtga cgcaggcggc gcacgacatc gcgcgccgcg tgctggaagg
     2221 gctggactat gtcggcgtgc tggcggtcga gctgttcgtg ctggacggcg accgtctcgt
     2281 gctgaacgag atcgcgccgc gcgtacacaa ttccggccac tggacgcagg aaggctgcgt
     2341 gtgcgaccag ttcgagcagc atgtccgcgc cgtggcgggc tggccgctgg gcgatccgtc
     2401 ggcgcgctgc acggtcgaga tgcgcaacct cctgggcgac gacatcctcg actggccgcg
     2461 cctcgccgcc gagccgaacg cgcaactgca cgtctatggc aaggccgaac cgaggccggg
     2521 ccgcaagatg ggccatatca accggatcaa aagctgatgt tccttctctc cctgacctac
     2581 aaggttccgc tcgacgtggt ggaccggcat ctcgacgccc atgtcgcgtg gctgaaggag
     2641 ggctatgcgt cgggcatgtt cctcgcctcg ggccgcaaga tcccgcgcac cggcggcatg
     2701 atcttcgcgc agggcgaacg cgcggcggtc gaagccttcg cgcggctgga cccgtttgcg
     2761 gtcaacggcg tggccgatta cgccgtcacc gaggtcaacc tgaccatgac cgccgagggg
     2821 ctggaggggt tgaaggggta gggtttctcg ctttaggaac aaaaaaaagc ggcccccgcc
     2881 ggagccgcct ttcctctatc ctgccccggc ctttacgccg cccgcagccg
//
LOCUS       DASVLL010000075         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_326238,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000075 DASVLL010000000
VERSION     DASVLL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326238"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF402_01285"
     CDS             complement(<1..559)
                     /locus_tag="VF402_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 36"
                     /protein_id="HEX7798950.1"
                     /translation="MIDFDSRRCRLTSATALPLAAGFLWNPRMMIHMTARGYATAQFM
                     QPEPSKYARAPIMEQAHFMLPEQGVYAHHPGRFFYVKDRETGGLFSAPHEPARAAPDA
                     FAFEVAPDGLSWRVDKDGVRVELRLGLPVDDTVELWELRLVNLDARPRRLSLYPAFPL
                     GLMSWMNQSADFDAELNGVVGRCVTG"
     gene            complement(556..1914)
                     /locus_tag="VF402_01290"
     CDS             complement(556..1914)
                     /locus_tag="VF402_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7798951.1"
                     /translation="MTSPPPTNQPGWFEKLSYGSGDFGFNLYWTTISAFLLIFYTDSF
                     GLNAAAAGTMLLTTKIVDAVADPVMGAVSDRTRTRWGRFRPWLLWGAVPLAASGVLTF
                     TTPNLDSGAKLIYAYITFALMMVIYSVVNIPYGALSGVLTSDGQARTQINSFRFVGGF
                     LGGTFTTYMTPKLVAWLGGADTVLGWQLTMGLYGLIAAALFFNLFRRTRERVTPAQDQ
                     AVSPWRDIADLTRNRPWMVLFALALIVMVTISTRMGASAYYMKYYVGQPERVAGFLTV
                     YGFALAAGSFLTPLLTRFVDKRTLMIVLLIAVGVLSSAFYFIPKDQIGLMYALQIAIG
                     LCLGPKSPLTFAMYADCADYTEWKTGRRATAMTFSAATFAQKLGGALASWVIGLVLAS
                     LGYVANAAQSHASQTGIVLLISVVPGVMALIAAVVMIFYNLSDQRLSAIQTELSQRKI
                     ST"
BASE COUNT          379 a          629 c          683 g          305 t
ORIGIN      
        1 aaccggtgac gcagcgcccg acgacgccgt tcagctccgc gtcgaaatcc gccgactggt
       61 tcatccagct catcaggccc agcgggaagg cgggatagag gctgagcctg cgcgggcgcg
      121 cgtcgagatt gacgaggcgc aactcccaca actcaaccgt atcgtccacc ggcaggccga
      181 ggcgcaactc gacgcggacg ccgtccttgt ccacccgcca actcaggccg tcgggcgcga
      241 cctcgaaagc gaaggcgtcc ggcgcagcgc gcgcgggttc gtgcggcgcg gagaacaggc
      301 cgcccgtttc gcggtccttg acgtagaaga agcggcccgg atggtgggcg taaacccctt
      361 gctccggcag catgaaatgc gcctgctcca tgatcggcgc gcgggcgtac ttgctcggct
      421 ccggctgcat gaactgggcg gtggcgtagc cgcgcgccgt catatggatc atcatgcgcg
      481 ggttccacag aaaaccggcg gccagcggca gcgccgtcgc ggaggtcagg cggcagcgcc
      541 gcgagtcgaa gtcgatcatg tggaaatctt gcgttggctc agttcggtct gaatcgcgct
      601 cagacgttgg tcggacaggt tgtagaagat catgacgacg gccgcgatca gcgccatgac
      661 gccgggaacg acgctgatca gcagcacgat gccggtctgc gaggcatggc tctgcgccgc
      721 gttggcgacg tagccgaggc tggccagcac gaggccgatc acccaagagg ccagcgctcc
      781 gcccagcttc tgcgcgaagg tggcggcgga gaaggtcatg gcggtggccc gccgcccggt
      841 cttccattcg gtgtagtcgg cgcagtcggc gtacatggcg aaggtcagcg gcgacttcgg
      901 gccaagacac agcccgatgg cgatctgcaa ggcgtacatc agcccgatct ggtccttcgg
      961 aatgaagtag aaggccgagg acagcacgcc gaccgcgatc agcagcacga tcatcagcgt
     1021 gcgcttgtcg acgaagcgcg tcagcagcgg cgtcaggaac gatccggccg cgagggcgaa
     1081 gccgtagacg gtcaggaaac cggcgacgcg ctccggctgg ccgacatagt atttcatgta
     1141 ataggccgag gcgcccatgc gcgtcgaaat ggtcaccatg acgatcaggg ccagggcgaa
     1201 cagcaccatc cacggccggt tgcgcgtcag gtcggcgatg tcgcgccacg ggctgacggc
     1261 ctggtcctgc gcgggcgtca cgcgttcgcg cgtccggcgg aacagattga agaacagggc
     1321 ggcggcgatc aggccgtaaa gccccatggt cagttgccag cccagcacgg tatcggcgcc
     1381 gcccagccac gcgaccagct tcggcgtcat ataggtggtg aaggttccgc ccagaaagcc
     1441 gccgacgaag cggaagctgt tgatctgggt gcgcgcctgc ccgtcgctgg tcagcacgcc
     1501 cgacagggcg ccgtagggga tgttgacgac ggaatagatc accatcatca gcgcgaaggt
     1561 gatgtaggcg tagatcagct tggcgccgga atcgaggttc ggcgtggtga aggtcagcac
     1621 ccccgacgcc gccagcggaa ccgcgcccca caacagccac ggccggaagc gcccccagcg
     1681 cgtgcgcgtg cggtcggaca ccgcccccat gaccggatcg gccacggcgt cgacgatctt
     1741 ggtggtcagg agcatggtcc cggcggcggc ggcgttgagg ccgaagctgt cggtatagaa
     1801 gatcagcaga aacgccgaaa tcgtcgtcca gtacaggttg aagccgaaat cgccggagcc
     1861 atagctgagc ttttcgaacc agccgggctg gttcgtcggg ggaggggacg tcatgcttct
     1921 caacctgtat atttctttgt tacgccaacg ctagcaggcc cgaaccggtt tgcaaagctg
     1981 ttcttcccct ctcccc
//
LOCUS       DASVLL010000076         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_165593,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000076 DASVLL010000000
VERSION     DASVLL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165593"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VF402_01295"
     CDS             complement(<1..578)
                     /locus_tag="VF402_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7798952.1"
                     /translation="MQDGFRHDPARMSRYRLKSEDLRLWSLVTATVRPHRPSAGSEPA
                     APEIETLAMPGMPPDKSVMRAQKPKPAPLTPFRIGEAVRPPSGFRLTRAGVIAPDPIE
                     PKRKRRISRERDPIEARLDLHGLNAIAAEARLKAFVQQAFANDYRAVLVITGKGVAET
                     GLLKRMAPEWLADPALSHIVAGLSPAHARHGGA"
     gene            complement(640..>1648)
                     /locus_tag="VF402_01300"
     CDS             complement(640..>1648)
                     /locus_tag="VF402_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798953.1"
                     /translation="GGAEGLTVMALPQAVSETPAQPESQPAAQDAEELADHRGAEAAR
                     AALGVGVWRYDPDSDAIVEDAALRRLIGRESEGGPTRIAEADQARADAAFGELMRGKR
                     DEIALDIPVAQDDGGTRWIALQGRATMTPDGQRSLYGVAYDVTRWREAPDAETAPVEA
                     APEAESEAETETEALQQRVAALEADLSESRNLLALLQRRYVALAETSAPSGDDAHEAR
                     IADLQSQLDAARAELQARQAPDPAPDPAPDPANEARLNALQAALDSAQRFETVGRLTH
                     DVALDFAQMLNVINGALEVMAKQKNSPENLRRLSEAALAAGRRGERLTRQLQAFQSEE
                     F"
BASE COUNT          196 a          585 c          597 g          270 t
ORIGIN      
        1 gcgccgccgt ggcgggcgtg ggcgggggac aggcccgcga cgatgtggga aagcgccggg
       61 tcggccagcc attccggcgc catgcgtttc aggaggccgg tttcggccac gcccttgccg
      121 gtgatgacca gcacggcgcg gtaatcattg gcgaaggcct gctgcacgaa cgccttgagc
      181 cgcgcctcgg cggcgatggc gttgaggccg tgcaggtcga gccgcgcctc gatcgggtcg
      241 cgctcgcggc tgatgcggcg cttgcgtttc ggctctatcg gatcgggcgc gatcacgccg
      301 gcgcgcgtca ggcggaagcc ggacggcggc cggaccgcct cgccgatgcg gaacggggtc
      361 agcggcgcgg gcttcggctt ttgcgcgcgc atcacgctct tgtccggcgg cataccgggc
      421 atggccaggg tctcgatttc gggcgcggcg ggttcggatc cggcggacgg cctgtgcggg
      481 cgcaccgtcg ccgtcaccag cgaccacagc cgcaaatcct cgcttttcaa cctgtatcgt
      541 gacatgcgcg ccggatcgtg acggaaacca tcctgcatct tcgcggatgc aggagaacga
      601 gacgcctaac ctacgtgatc cgcggcgcgg cgtcagcttt taaaactctt ccgactgaaa
      661 cgcctgcaac tgccgcgtca ggcgttcgcc gcgcctgccg gcggccagcg cggcctcgga
      721 caggcggcgc aggttttccg ggctgttctt ctgcttcgcc atgacttcga gcgcgccgtt
      781 gatcacgttc agcatctggg cgaagtccag cgccacgtcg tgggtcaggc gcccgaccgt
      841 ctcgaagcgc tgggcgctgt cgagcgccgc ctgcaaggcg ttcaggcgcg cctcgttggc
      901 cggatcgggg gccggatcgg gggccggatc gggcgcctga cgcgcctgaa gctcggcccg
      961 cgccgcgtcg agctggcttt gcaggtcggc gatgcgcgct tcgtgagcgt cgtcgccgga
     1021 aggcgcggag gtttcggcca gcgccacata gcgccgctgc aacagggcca gcaggttgcg
     1081 gctttcgctc aggtccgcct ccagcgccgc gacgcgctgt tgcaaagctt ccgtttcggt
     1141 ttcggcctcg gactcggcct ccggggcggc ttcgaccggc gccgtctccg cgtccggcgc
     1201 ctcgcgccag cgcgtgacgt cataggccac gccgtagagg ctgcgctgtc cgtccggcgt
     1261 catggtcgcg cggccctgca aggcgatcca gcgggttccg ccgtcgtcct gcgccaccgg
     1321 aatgtcgagc gcgatctcgt cgcgcttgcc gcgcatcagc tcgccgaacg ccgcgtcggc
     1381 gcgcgcctga tccgcctcgg cgatgcgcgt cgggccgccc tcgctctcgc gtccgatcag
     1441 gcggcgcagg gccgcgtcct cgacgatggc gtcggagtcg ggatcgtagc gccagacccc
     1501 gacgcccagc gccgcgcgcg ccgcctcggc gcctcggtgg tcggccagtt cctcggcgtc
     1561 ctgcgccgcc ggttgcgatt ccggctgagc gggcgtttcg gaaaccgcct gcggcagggc
     1621 catcacggtc aggccctcgg cgccgccc
//
LOCUS       DASVLL010000077         5497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_165707,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000077 DASVLL010000000
VERSION     DASVLL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165707"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VF402_01305"
     CDS             <1..1561
                     /locus_tag="VF402_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7798954.1"
                     /translation="PSAGDAAFARLGHQADALDLSAVAPGATASALPAGWSRNAQIME
                     IFVRSYKDSDGDGIGDLKGLTSQLDYLKSLGVTGLWLMPIYRSGDHDHGYAVADYRSV
                     DPDYGTMADFDTLVAEAHKRGIGIILDYVMNHASSQNPLFLDAVSSTANSRRDWFLWN
                     ASDPGWSAFNGASWHVSESGYYYGIFSTDMPDWNLTNAAVVDYHLDNLRFWLNRGVDG
                     FRFDGAEYLIENGPNAYMNQPQTLSLLQRAKAVIDGYANRYMVCEAPDAPDDFGTACG
                     HAFAFGHQDDIKTSAASGKAAAGLIAYLNAPDRAQLPLFLSNHDAFAGARPYPDLSGH
                     GEGDYRAAAAIELLASDTPFTLYGEEVGMADNADSGDNAIRAPMSWTGDAATAGFTTG
                     TPYRGLSVNAATHNVAAEQGVAGSLLETYRGLYAARSHNPVLQSGTLTPLSAAGQSYV
                     AFLRQSGGKTAVVAINLSATAQTVTLNTGLAAQTFTGAYPNVGGSHTADAGGKLSLDL
                     PAEGAVVVTTP"
     gene            2042..5023
                     /locus_tag="VF402_01310"
     CDS             2042..5023
                     /locus_tag="VF402_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7798955.1"
                     /translation="MKSAFLMNAKRASVLMGGASLLAIGLTAAPAFAQDAAQNAAPAK
                     DDNTVVVVTGQRAQLRSAQKIKKDSPVIVDSITAVDIGALPDRSVSEALQRISGLTLE
                     RTNNNRDPARLAVEGGNVEIRGLSWVKSETNGRDIFSANNGRALSFDDISADLLAGVD
                     VYKNPSASMIEGGIGGIVDLRTRLPFDARKQVVAFSVDSNYGDLDKKPHPSINGFYSN
                     RWHTAAGEFGLLVDLSEVNVGNRTDSLSLDKFNATTVSSGNAVTSTDDGKVHNADGSD
                     KTFYIPGSIGWREVTWNAKRSDATVALQYRPNDAWLFTLNYLNVRSREDNTEYALGSY
                     DGSLTSPQPSWNYQYDSNGVLMAGTNPSAGYDADTRYELDHKNTADTSLNVKFTPNAN
                     LTLTGDLQYVESSADMTSMTAYTELGTKTSISFSGLQSDMPTITVNDANPDTASNYYW
                     AAAMDHLEHNTGQQLAARFDGDYHFDTGWLRELKAGVRVTDKHYVTRQTGWNWSLLSQ
                     QYWSPAAGQSGYYPPVYITSTDSSMVTYQTFGNFFHGDANVPGNVWFPSASVVNQGTA
                     HTYDLLKSTETAGWGWTPLAADAWNNYNPEADNQSAGVNRQEEKTAAAYAEASFGGAN
                     LFGHDYDGNFGLRYVQTEAIQDGGVVAFTAPQTACTATDCSAYDAMIAFAAGGITGGY
                     AGASDHKYSNWLPSFNLRVHLSDNWQLRFGASRAMVRPDPSQLINYTTLAFNQNSDGT
                     PNPNATGGAYTGTGGNPFLKPITANQYDLTAEYYFSPTGSVTFDLFDKELQNYIYTGT
                     DSETYTSNGQSVTFNVQRQFNGYKGSVKGFEVAYQQFFDFLPSVWSGLGVQANYTHLN
                     STGGHNTAANVYDSNQITGQNQDLPLEGMSPDSYNFAAMYEKYGISARLAYNWRSTYL
                     LTTSAANVNAPVWSEHYGQIDGSIFYTINKNYKIGVQATNLTHAKTILDVSTPTDISI
                     REHYNWVDTDRRIAIVLRGTF"
     gene            5126..>5497
                     /locus_tag="VF402_01315"
     CDS             5126..>5497
                     /locus_tag="VF402_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX7798956.1"
                     /translation="MPPFIERLIIAGGGTAGWMTAALLRSALPANVEIVLIESEDIAA
                     VGVGEATIPTIRLFNRQIGVNDVAFVQATEATFKLGVEFVGWGHEGNRYFHPFCDYGD
                     SFEGISAHQIWRRLNLAGDDAP"
BASE COUNT         1088 a         1931 c         1576 g          902 t
ORIGIN      
        1 gccgagcgcg ggcgacgccg ccttcgcgcg cctcggccat caggcggacg cgctcgacct
       61 gtccgccgtg gcgcccggcg cgacggccag cgccctgccc gccggctgga gccgcaacgc
      121 gcagatcatg gagatcttcg tgcgctccta taaggactcc gacggcgacg gcatcggcga
      181 cctcaagggc ctgacctcgc agctcgacta cctgaaaagc ctcggcgtca ccggcctgtg
      241 gctgatgccg atctacagga gcggcgacca cgaccacggc tacgccgtcg ccgactaccg
      301 cagcgtcgat cccgactacg ggacgatggc ggatttcgac acgctggtcg ccgaggcgca
      361 caagcgcggc atcggcatca tcctcgacta tgtcatgaac cacgcctcca gccagaatcc
      421 gctctttctc gacgccgtgt cctcgaccgc gaacagccgc cgcgactggt tcttgtggaa
      481 cgccagcgat ccgggctgga gcgcgttcaa cggcgcctcg tggcatgtct ccgagagcgg
      541 ctattattac ggcattttct cgaccgacat gccggactgg aacctgacca acgccgccgt
      601 ggtcgactat cacctcgaca atctgcgctt ctggctcaat cgcggcgtcg acggcttccg
      661 cttcgacggc gcggaatacc tgatcgagaa cggcccgaac gcctatatga accagccgca
      721 gaccctgagc ctgctgcaac gggccaaggc cgtgatcgac ggctacgcca accgctacat
      781 ggtctgcgaa gcgcccgacg cgccggacga tttcgggacc gcctgcggcc acgccttcgc
      841 cttcggccat caggacgaca tcaagacctc cgctgcgtcc ggcaaggcgg cggcgggcct
      901 gatcgcctac ctgaacgcgc cggaccgcgc gcagttgccc ctgttcctct ccaaccacga
      961 cgccttcgcg ggcgcgcggc cctatcccga cctgagcggg catggcgaag gcgactaccg
     1021 cgcggcggcg gccatcgagc tgctggcctc ggacacgccg ttcacgctct acggcgagga
     1081 ggtcggcatg gcggacaacg ccgacagcgg cgacaacgcc atccgcgccc cgatgagctg
     1141 gaccggcgac gcggcgaccg ccggcttcac cacggggacg ccctatcgcg gcctgtccgt
     1201 caacgccgcc acgcacaatg tcgcggcgga acagggcgtt gccggctcgc tgctggagac
     1261 ctatcgcggc ctctacgccg cgcgcagcca taaccccgtg ctgcaatcgg gaaccctgac
     1321 gcctttgtcg gcggcgggcc agtcctatgt cgccttcctg cgccagtcgg gcggcaagac
     1381 cgccgtggtc gcgatcaacc tgtcggccac ggcgcagacg gtgacgctga acaccggcct
     1441 cgccgcgcaa accttcaccg gcgcctatcc gaacgtcggg ggatcgcaca ccgccgatgc
     1501 tggcggcaag ctcagcctcg acctgcccgc tgagggcgcg gtcgtcgtga cgacgcccta
     1561 agcgtcgggc gcggcggttt cgcaaccgtc ccggcggcgt cgcgccgccg ggacggattt
     1621 ccatttccgt tttgcctgca atgttatttt cgcatgtaaa aatcatctca tatgccccgt
     1681 cgcgaataaa aatttatgca taaaaacaat gttttgtgtt gcaggattac tataggcagc
     1741 gctgacatcg agaggccaag gctgcaaatc tggccattat catacaaaat atggtagcgt
     1801 tcccgaagcg cccggcgtct caggacacgg cccaacaggg ccggcatgag accggggccg
     1861 ccacaaataa agtatgggaa gacgtcacag ccgccggata acgatccgct ttttttgcgc
     1921 tttgtttccg tttttacata taaatacggt tttaccggca ctcacgtctc cctgtccgcg
     1981 tacattttcc ctcccgcctg agacacaggc cggaggccac tcatcaggga gccaaccgcc
     2041 tatgaagtcc gctttcttaa tgaacgccaa gcgcgcctcc gtcctcatgg gcggcgcctc
     2101 gctgctggcc atcggcctga ccgccgcgcc cgccttcgcg caggacgccg ctcaaaacgc
     2161 cgcgcctgcc aaggacgaca acaccgtcgt cgtcgtcacc ggccagcgcg cccagctcag
     2221 aagcgcgcag aagatcaaga aggactcgcc ggtcatcgtc gattccatca ccgccgtcga
     2281 catcggcgcc ttgccggacc gttcggtgtc cgaagccctg caacgcattt ccggcctgac
     2341 gctggagcgc accaacaaca atcgcgatcc ggcccgtctg gctgtcgaag gcggcaatgt
     2401 cgaaatccgc ggtctgtcgt gggtcaagtc cgaaaccaac ggacgcgaca tcttctcggc
     2461 caataatggc cgcgccctga gcttcgacga tatttccgcc gatctgctgg cgggcgtcga
     2521 cgtttacaag aacccgtcgg cctcgatgat cgaaggcggc atcggcggca tcgtcgacct
     2581 gcgcacgcgc ctgcccttcg acgccaggaa gcaggtcgtg gccttctcgg tcgacagcaa
     2641 ctacggcgac ctcgacaaga agccgcatcc gtcgatcaac ggcttctatt ccaaccgctg
     2701 gcataccgcc gccggcgagt tcggcctcct ggtcgacctg tcggaagtca atgtcggcaa
     2761 ccgcacggac agcctgagcc tggacaagtt caacgccacg acggtttcca gcggcaacgc
     2821 ggtcacgagc accgacgacg gcaaggtcca caacgccgac ggctcggaca agaccttcta
     2881 catccccggc tcgatcggct ggcgcgaagt gacatggaac gccaagcgca gcgacgccac
     2941 ggtggcgctg caataccgcc cgaacgacgc gtggctgttc accctgaact atctcaacgt
     3001 gcgttcgcgc gaagacaaca ccgaatatgc gctgggcagc tacgacggtt cgctcacctc
     3061 gccgcagccg agctggaact atcagtatga ctcgaacggc gtgctgatgg ccgggaccaa
     3121 cccgtcggcg ggctatgacg ccgatacgcg ctatgagctg gaccacaaga acaccgcgga
     3181 cacgtcgctg aacgtcaagt tcacgccgaa cgccaacctg accctgaccg gcgatctgca
     3241 atacgtcgaa tcctcggccg acatgaccag catgaccgcc tacaccgaac tggggacgaa
     3301 gacgtcgatc tccttctccg gcctgcaaag cgatatgccg accatcacgg tcaacgatgc
     3361 gaatccggac accgccagca actactactg ggccgcggcg atggaccatc tggaacacaa
     3421 caccggtcag caactggcgg cgcgcttcga cggcgactac catttcgaca ccggctggct
     3481 gcgtgagctg aaggcgggcg tgcgcgtcac cgacaagcat tacgtcaccc gccagaccgg
     3541 ctggaactgg agcctgctga gccagcaata ctggagcccc gccgccggcc agtccggcta
     3601 ctatccgccg gtgtacatca ccagcaccga ttccagcatg gtcacctacc agaccttcgg
     3661 caacttcttc cacggcgacg ccaacgtgcc gggcaatgtc tggttcccgt cggcctcggt
     3721 cgtcaatcag ggcacggccc acacctacga cctgctgaaa agcaccgaga cggcgggctg
     3781 gggctggacc ccgctggccg ccgacgcctg gaacaactat aaccccgaag ccgacaacca
     3841 gtcggcgggc gtgaaccgtc aggaagaaaa gaccgccgcc gcctatgccg aagccagctt
     3901 cggcggcgcc aatttgttcg gccacgacta tgacggcaat ttcggcctgc gctacgtgca
     3961 gaccgaagcc atccaggacg gcggcgtcgt cgccttcacg gcgccgcaaa cggcctgtac
     4021 ggcgaccgac tgctcggcct atgacgccat gatcgccttc gcggccggcg gcatcaccgg
     4081 cggttacgcg ggcgcttcgg atcacaaata ttccaactgg ctgcccagct tcaacctgcg
     4141 cgtccacctg agcgacaact ggcaactgcg cttcggcgcc tcgcgcgcca tggtccgccc
     4201 ggatccgagc cagttgatca actacaccac cctggccttc aaccagaact cggacggcac
     4261 gccgaacccg aacgccacgg gcggcgccta taccggcacc ggcggcaatc cgttcctgaa
     4321 gccgatcacc gcgaaccagt acgacctgac ggcggaatac tatttctccc ccaccggcag
     4381 cgtcaccttc gacctgttcg acaaggaact gcaaaactac atctatacgg gcacggattc
     4441 cgaaacctac acctcgaacg gccagagcgt gaccttcaac gtgcagcgcc agttcaacgg
     4501 ctacaaggga tcggtcaagg gcttcgaggt cgcctaccag cagttcttcg acttcctgcc
     4561 cagcgtctgg tccggtctcg gcgtgcaggc caactacacc cacctgaact cgaccggcgg
     4621 ccacaacacg gcggccaacg tctatgacag caaccagatc accggccaga accaggatct
     4681 gccgctggaa ggcatgtcgc cggacagcta caatttcgcg gcgatgtacg aaaagtacgg
     4741 catttcggcg cgcctggcct acaactggcg ctcgacctac ctgctgacga cgtcggccgc
     4801 caacgtcaac gcgccggtct ggtccgagca ttacggccag atcgacggct cgatcttcta
     4861 cacgatcaac aagaactaca agatcggcgt acaggcgacc aacctgaccc acgccaagac
     4921 cattctggac gtaagcacgc cgaccgacat ctcgatccgc gagcattaca actgggtgga
     4981 caccgaccgc cgcatcgcca tcgtgctgcg cggcacgttc tagctcctcg cgaacttctc
     5041 gaccttgcgg acattcgcac cccctttccg ccacaacggg aagggggtat ttttttataa
     5101 gatgcaggat tttaaagata acgccatgcc gccttttatc gaacgcctga tcatcgccgg
     5161 cggcggaacc gccggatgga tgaccgccgc cctgctgcgc tcggccctgc cggcgaatgt
     5221 cgagatcgtg ctgatcgaat ccgaggacat cgccgctgtc ggggtcggcg aggccaccat
     5281 cccgaccatt cgcctgttca accgccagat cggcgtcaac gacgtggcct tcgtccaggc
     5341 caccgaagcc actttcaagc tcggcgtcga attcgtcgga tggggccatg aaggcaatcg
     5401 ctacttccat cccttttgcg attacggcga ctccttcgaa gggatttccg cgcaccagat
     5461 ctggcggcgg ctgaacctgg cgggcgacga cgcgccg
//
LOCUS       DASVLL010000078         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_434166,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000078 DASVLL010000000
VERSION     DASVLL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434166"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VF402_01320"
     CDS             <1..329
                     /locus_tag="VF402_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798957.1"
                     /translation="PRPSFPPHPITMASDARTPQEPLTPEARRDLLRAFADRMLLKIT
                     AMDDPEDLPGVERAVRAAAVIERLYRSCDRAERHADDPRRLRAERAQYESEAIKAQVS
                     LAGALK"
     gene            complement(570..743)
                     /locus_tag="VF402_01325"
     CDS             complement(570..743)
                     /locus_tag="VF402_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798958.1"
                     /translation="MDPAAERMKCVMEAIDHGLTVNETLEECPHCAPQPDAIGLHQER
                     WDFWAPTSFSETL"
     gene            959..>1601
                     /locus_tag="VF402_01330"
     CDS             959..>1601
                     /locus_tag="VF402_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEX7798959.1"
                     /translation="MSQTRILVFGNEKGGAGKSTVAMHVGVHLLHQGHRVAFLDLDLR
                     QRSLARFFSNRRQWAQANGKTLPQAFEPFLHARPAELLNAPESEAKAAFDRALEEALD
                     GADYVIIDTPGGDHALSRRAHAAAHVIVTPMNDSFIDFDLLGEVDPVTLDVKRPSIYA
                     ETVWNSRKQRAVWDGKSIRWIVLRNRLAQLEARNRRRVDERVQALGRRVGFEVL"
BASE COUNT          293 a          546 c          517 g          245 t
ORIGIN      
        1 atccccgccc gtcatttccg ccgcatccta taacgatggc ctcggatgcg cgcacaccac
       61 aggagccttt gacgccggaa gcgcggcgcg acctgctgcg cgccttcgcc gaccggatgc
      121 tgctgaaaat caccgcgatg gacgaccccg aagacctgcc gggcgtcgaa cgcgccgtgc
      181 gcgccgccgc cgtgatcgaa cgcctctaca gaagttgcga ccgcgccgag cgccacgcgg
      241 acgatccgcg caggctgcgg gccgagcgcg cgcaatacga gagcgaagcc atcaaggcgc
      301 aggtctcgct ggccggcgcg ctgaaataga gcgccgaacg ccgccgcgac ctcggccaat
      361 ggtgggacgc cgccggcgaa gcggcgcagg ctgcgaagcc cgcgcaaaag cccgccgaaa
      421 atcccgaagc gccccatgca gccaagccat cgacggcgcc gcaagtcgcc tgcgccgccg
      481 acgacgacgc agcggccgct caggccgtgc cgccatttca gccggaacac gcccggaccc
      541 gctttccgcc ctaaacggaa aggcggcgcc tacagcgtct cggaaaacga tgtcggcgcc
      601 cagaagtccc agcgttcctg atgcagaccg atggcgtccg gttgcggcgc acaatgagga
      661 cattcctcca gcgtctcgtt gaccgtgagg ccgtgatcga tggcctccat cacgcacttc
      721 atccgttccg cggctgggtc cattgctgga tctcccttct gagtgacgga ccgagtgtgc
      781 gcctgaaccc cagggttgga aaatcacatt tgtttgcaca tgcgaacatc cgcatgcggg
      841 cgccgcgacg cccatttacc cacgacaata tgttaaccaa agattgacgc gctctggcgg
      901 cggagggcaa gagcgcttaa gtctggtgga caatttgagt cggcgggagt gggagttcat
      961 gtcgcaaacg cgcattctgg tgttcggcaa cgaaaagggc ggggcgggca agtcgaccgt
     1021 cgccatgcac gtcggcgtgc atctgctgca tcagggccat cgcgtcgcct ttctcgatct
     1081 cgacctgcgg caaagatcgc tggcgcgctt cttctccaac cgcaggcagt gggcgcaggc
     1141 caatggcaag accctgccgc aggcgttcga gccgttcctg cacgccaggc ccgccgaact
     1201 gctgaacgcg cccgaatcgg aggcgaaggc cgcgttcgac cgcgcgctgg aagaggcact
     1261 ggacggcgcc gattacgtca tcatcgacac gccgggcggc gaccacgcct tgtcgcgccg
     1321 cgcgcacgcc gccgcgcacg tcatcgtcac gccgatgaac gacagcttta tcgatttcga
     1381 cctgctgggc gaggtcgatc cggtcacgct cgacgtcaag cgcccgtcga tctacgccga
     1441 gacggtgtgg aattcacgca agcaacgcgc ggtgtgggac ggcaagtcga tccgctggat
     1501 cgtgctgcgc aaccgtttgg cgcagcttga ggcgcgcaat cgccgccgcg tcgatgagcg
     1561 cgtgcaggcg ctgggccgcc gcgtcggctt cgaggtgctg g
//
LOCUS       DASVLL010000079         5207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_434251,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000079 DASVLL010000000
VERSION     DASVLL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434251"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VF402_01335"
     CDS             <1..630
                     /locus_tag="VF402_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7798960.1"
                     /translation="LPDYLLTGGGACSLGPGAGAPCPAQPDTLFSVTRTLNTRGGPID
                     GLEINYQQGLNFLPGRLRNIGVLFNYTHITSSVEYVTALDDPRTAADEGRTVRGDFPG
                     MSRNALNLTVYYEKRGFSGRVSISQKSRAINAALGDVQGADFTFTDPATYVDASFAWR
                     LGPHLRLSLEGANLTDQPVRFGKDTTRDDTLLYVHSGRTMTLGLNWRFN"
     gene            720..3485
                     /locus_tag="VF402_01340"
     CDS             720..3485
                     /locus_tag="VF402_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7798961.1"
                     /translation="MTHVTRLKPNLMLGAGLAALMLAPGLACAQDANADQGKDSTVVV
                     VTGIRAALRSAMNVKRDSDKVMDAITAEDIGKFPDKNLGESLQRVTGVQITRQDGEGR
                     GVSIRGADPSLVRTEIDGATALSLTVGGTDRAVDFRDLPVEFVSRLEVVKSVTPDMTE
                     GGLGGTVRVVMRKPFDSRKPFLAASAQGVYSSLAKTYDPKFSLIGSRQFFDGRLGVLA
                     SVNYEDRHLYNNKSLTTGWVRKVDLNKDGVLDWTPDIPRPDIERRETTRLAYNTIVQW
                     RPNERMEFYVEGNYAQGHEVVDNMLLQLSASSGVVDVSKTTLAATPDAIGAYTVDHVE
                     LVSDATHAMQLQYRNIDGTLTRTQWDAAVGGKWNATDRLEIDARYAYSAGKVRNMEKD
                     TQAGILAVPRAIIDYANSQLTPNMSFTDAAGHPLDVTTGALVDRLDATFTPVTDTASE
                     SDVKLDAEYHPDSPWLTAVKFGFEGHDTQTVQDFWSRVTTLTSAGSVKSSGKTLVYYT
                     DQASVANIVDTYSGTNKEPFFSGGDLGYSGGILHWNDNGLATWQAILDAAGVTDGDSV
                     DAANPNANTGGSYRQWTSNFDVREKTTAFYLQASFAWPVFGVPMSGVIGDRVIKTDTT
                     STGFGQTTNADKSLSFPVVSQDGSYSNDLPSLNLKFDFLPRKLIGRFSIGKVMARPAP
                     SQLSFAQTLDVVGLKGSQGNPNLKPYLATNTDLGIESYFSKDGFVSAALFEKDISRFI
                     INTTIPYLANDGQTYSLTLPVNGTDRVKVSGLELGGQYAFSMLPAPFDGFGVLGNVTL
                     QKDRGFKGTNLLTGELLPYPGLSRTSYNAGVYYEDARISARLSYNWRSRWLITASGRG
                     GLPEYNDAFGTMDMSVDYNLTKQVTVFVDGVNLTNAAYIQENDPLRRLSNETDGLKVF
                     FGVRFRN"
     gene            3715..4152
                     /locus_tag="VF402_01345"
     CDS             3715..4152
                     /locus_tag="VF402_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7798962.1"
                     /translation="MTKTGTKTGTKTGPLRILVVDDNRETASLITESLHDLGAEAEAC
                     LSGEEALAHIAGFRPQILMLDLDMPGMDGFEVAQTLRARPDMGAARIIAQTAYGDADM
                     RARTARAGFDLHLTKPLSLARLGDMLDLLTSALGREGEDWATA"
     gene            4260..>5207
                     /locus_tag="VF402_01350"
     CDS             4260..>5207
                     /locus_tag="VF402_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798963.1"
                     /translation="MFASSRPARALIPVLALLVLAPAAAAAGALKNAVEVFPFPQPIR
                     YDHHNDDFTVRVRTPGGVWQDLYEYNVKVDLDKPQDASFVYFDFDGTVEVSVQKNNED
                     VRSVRVRPDSKGIRARLENGTAYFTLTKPENLSVEFDGDHLHNLHLFSHAIRKDMPAH
                     PAMTSYEIGAAPNPDLKGPVVTFGPGVYTPPDGPGGSYRIGSNTTVYVDGSALIRGTF
                     VIENAENVRIVSDGLFEGQKDLLWVRNSRHVEIDGPIVFNPVHGSMRCAQSQDVTFRD
                     IRIIGGGPWSDGIGNFACQNVTIADSFIRTSDDSVTIYNH"
BASE COUNT          961 a         1722 c         1676 g          848 t
ORIGIN      
        1 ctgcccgact atctgctgac cggcggcggc gcctgctcgc tcggccccgg cgcgggcgcg
       61 ccctgtccgg cccagcccga caccctgttc agcgtcacgc gcacgctcaa cacgcgcggc
      121 gggccgatcg acgggctgga gatcaattac cagcaggggc tgaacttcct gccggggcgg
      181 ctgcgcaata tcggcgtgct gttcaactat acgcacatca cgtcgtcggt cgaatacgtc
      241 accgcgctcg acgatccgcg caccgccgcc gacgagggcc gcaccgtgcg cggcgacttt
      301 cccggcatgt cgcgcaacgc gctgaacctg accgtctatt acgaaaagcg cggcttcagc
      361 gggcgcgtct cgatctcgca gaagtcgcgc gccatcaacg ccgcgctcgg cgacgtgcag
      421 ggggccgact tcaccttcac cgacccggcg acctatgtcg acgccagctt cgcctggcgg
      481 ctggggccgc atttgcgcct cagcctcgaa ggtgccaacc tgaccgacca gccggtgcgc
      541 ttcggcaagg acacgacgcg cgacgacacc ttgctctacg tccattccgg ccgcacgatg
      601 acgctggggc tgaactggcg cttcaactga cttttctttg agggctttcg tctcgtgtcc
      661 gtcgaacagg atgagcgcgc gtataagcgc gccgtcatcc gggcaagagg aacaaggcca
      721 tgacacacgt tacaagactg aagccgaacc tgatgctggg ggcgggcctc gccgcgctga
      781 tgctggcgcc ggggctggcc tgcgcgcagg acgccaacgc ggatcagggt aaggactcga
      841 ccgtggtggt cgtgaccggc atccgcgcgg cgctgcgttc ggccatgaac gtcaagcgcg
      901 attccgacaa ggtgatggac gccatcaccg ccgaggacat cggcaagttc ccggacaaga
      961 atctcggcga atcgctgcaa cgcgtgaccg gcgtgcagat cacccgtcag gacggcgagg
     1021 ggcggggcgt gagcatccgc ggcgccgatc cgtctttggt gcgcaccgag atcgacggcg
     1081 cgacggcgct gtcgctgacc gtcggcggca cggaccgcgc ggtcgatttc cgcgacctgc
     1141 cggtcgagtt cgtcagccgc ctcgaagtcg tcaagtcggt gacgccggac atgaccgagg
     1201 gcggcctcgg cggcacggtg cgcgtcgtga tgcgcaagcc gttcgattcc agaaagccct
     1261 tcctcgccgc ctcggcgcag ggcgtctatt ccagcctcgc caagacctac gatccgaagt
     1321 tctcgctgat cggctcgcgc cagttcttcg acggcaggct gggcgtgctg gccagcgtca
     1381 attacgagga ccgccatctc tacaacaaca agagcctgac gaccggctgg gtgcgcaagg
     1441 tcgacctgaa caaggacggc gtgctggact ggacgcccga cattccgcgc ccggacatcg
     1501 agcggcgcga gacgacgcgg ctggcctaca acaccatcgt gcagtggcgg cccaatgaac
     1561 gcatggagtt ctatgtcgag ggcaattacg cgcaaggcca cgaggtcgtc gacaacatgc
     1621 tgctgcaact gtcggcctcg tccggcgtgg tggatgtgag caagacgacc ctggccgcga
     1681 cgccggacgc catcggcgcc tatacggtcg atcatgtcga gctggtcagc gatgcgacgc
     1741 acgccatgca actgcaatac cgcaatatcg acggcacgct gacgcgcacg caatgggacg
     1801 cggcggtcgg cggcaagtgg aacgcgaccg accggctgga gatcgacgcg cgctacgcct
     1861 attcggcagg caaggtgcgc aatatggaaa aggacacgca ggccggcatc ctggccgtgc
     1921 cgcgcgccat catcgactac gccaacagcc agttgacgcc gaacatgtcg ttcaccgacg
     1981 cggcgggcca tccgctggac gtgaccacgg gcgcgctggt cgaccggctg gacgcgacct
     2041 tcacgccggt caccgatacg gccagcgagt ccgacgtgaa gctcgacgcc gaatatcatc
     2101 cggattcgcc gtggctgacc gcggtgaagt tcggcttcga gggccacgac acccagaccg
     2161 tgcaggactt ctggtcgcgc gtgaccaccc tgacctcggc cggcagcgtc aaaagctccg
     2221 gcaagaccct ggtctattac accgatcagg ccagcgtcgc gaacatcgtc gatacctatt
     2281 cggggaccaa taaggagccg ttcttctccg gcggcgatct cggctattcc ggcggcatcc
     2341 tgcactggaa cgacaacggg ctggcgacgt ggcaggcgat cctcgacgcg gcgggggtga
     2401 cggacggcga ttcggtcgac gccgccaatc cgaacgccaa caccggcggc agctaccgcc
     2461 agtggaccag caatttcgac gtgcgcgaaa agacgaccgc cttctatttg caggcttcgt
     2521 tcgcctggcc ggtgttcggc gtgccgatga gcggcgtgat cggcgaccgg gtgatcaaga
     2581 ccgacaccac ctcgaccggc ttcggccaga ccaccaacgc cgacaagtcg ctgagcttcc
     2641 ccgtcgtgtc gcaggacggc tcctacagca acgacctgcc gtcgctgaac ctgaagttcg
     2701 acttcctgcc gcgcaaactg atcgggcgtt tcagcatcgg caaggtgatg gcccgtccgg
     2761 cgcccagcca gttgtccttc gcccagaccc tggacgtggt gggcctgaaa ggctcgcagg
     2821 gcaatccgaa cctcaagccc tatctggcga ccaataccga cctggggatc gaatcctatt
     2881 tcagcaagga cggcttcgtc tcggcggcgc tgttcgagaa ggacatttcg cgcttcatca
     2941 tcaacaccac gatcccgtat ctggccaatg acggccagac ctacagcctg accctgccgg
     3001 tcaacgggac ggaccgggtc aaggtgagcg gcctcgaact gggcggccag tacgccttct
     3061 cgatgctgcc cgcgccgttc gacggcttcg gcgtgctggg caacgtcacc ttgcagaagg
     3121 atcgcggctt caaggggacg aaccttttga ccggcgagct tctgccctat ccgggcctgt
     3181 cgcgcaccag ctacaatgcg ggcgtctatt acgaggacgc caggatcagc gcgcggctca
     3241 gctacaactg gcgctcgcgc tggctgatca ccgcgtcggg acgcgggggc ctgccggaat
     3301 ataacgacgc gttcgggacg atggacatgt cggtcgatta caacctgacg aagcaggtga
     3361 ccgtctttgt cgacggcgtg aacctgacca acgcggccta tattcaggag aacgatccgc
     3421 tgcgccgcct gtccaacgag acggatgggc tgaaggtctt tttcggcgtg cgcttccgca
     3481 actgatcgag ggcgggggtc gccggagcga ggggcgggac cgcctttcgc tccggcgccg
     3541 ttgcgggcgc gtcttgcgcg tggcggaaac gctcgatctg tcgccgaaca tgcggcgcgg
     3601 ggcgacggac gctcacgaaa aatttgactg aaatcagccg cttttccgcg ccccgccgta
     3661 atgtcttcgc cacattacgc gggtaaagcc gggggcgaag atacggaggc ggtgatgacg
     3721 aagacgggga ccaagacggg gacgaagacg gggcccttgc gcatactggt ggtcgacgac
     3781 aaccgcgaaa ccgccagcct gatcacggag tccctgcacg atctgggcgc cgaggccgaa
     3841 gcctgcctgt cgggcgagga ggctctggcg cacatcgccg ggttccggcc gcagatcctg
     3901 atgctcgacc tcgacatgcc gggcatggac ggcttcgagg tcgcgcagac cctgcgcgcc
     3961 cgcccggaca tgggcgcggc ccggatcatc gcccagaccg cctacggcga cgccgacatg
     4021 cgcgcccgca ccgcgcgcgc gggcttcgac ctgcacctga ccaagcccct gagcctcgcc
     4081 cgcctcggcg acatgctcga cctcctgacc agcgcgctgg gacgggaagg cgaggactgg
     4141 gcgacggcgt aggcggaatt cttacgagcg ggcatgtatg gcttgcataa ggccgcgcga
     4201 atgtgatatc gatatcaaaa tccgtcgcca ggaacggcct ttgacagcag gaaaaaccca
     4261 tgttcgcatc ttcacgcccg gcccgcgccc tgattccggt attggcgctg ctcgttcttg
     4321 cgcccgccgc cgccgcagcc ggggcgttga aaaacgcggt ggaggttttt ccgttcccgc
     4381 agcccatacg ctacgaccat cataacgacg atttcacggt gcgcgtgcgc acgcccggcg
     4441 gggtctggca ggatctttac gagtacaatg tcaaggtcga cctcgacaag ccgcaggacg
     4501 cctccttcgt ctatttcgac ttcgacggga cggtcgaggt gtccgtgcag aagaacaatg
     4561 aagatgtccg ctcggtccgg gtgcggcccg attccaaggg gatcagggcg cggctcgaaa
     4621 acgggaccgc ctatttcacg ctgacgaaac cggaaaacct gagcgtggag ttcgacggcg
     4681 atcacctgca caatctgcac ctgttcagcc acgccatccg caaggacatg cccgcgcatc
     4741 cggccatgac cagctacgaa atcggcgcgg cgccgaatcc cgacctgaaa gggccggtcg
     4801 tgaccttcgg gccgggcgtc tatacgccgc cggacggtcc gggcggcagc tacaggatcg
     4861 gctcgaacac cacggtctat gtcgacggct cggcgctcat ccgggggacg ttcgtgatcg
     4921 agaacgccga gaacgtccgc atcgtcagcg acggcctgtt cgaggggcag aaggatctgt
     4981 tgtgggtgcg caattcccgg catgtcgaga tcgacgggcc gatcgtcttc aatcccgtgc
     5041 atggctcgat gcgctgcgcg cagtcgcagg acgtgacgtt ccgcgacatc cgcatcatcg
     5101 gcggcggccc gtggtcggac ggcatcggca atttcgcctg tcagaacgtg acgatcgccg
     5161 attccttcat ccgcacctcg gacgacagcg tcaccatcta taaccac
//
LOCUS       DASVLL010000080         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_4663, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000080 DASVLL010000000
VERSION     DASVLL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4663"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VF402_01355"
     CDS             <1..751
                     /locus_tag="VF402_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798964.1"
                     /translation="PDSVKPDRGAYAPALIGAGAAAVLLAAAAFGAATYAQSLNKDQN
                     AARAQPVFRLRKPLPPARVEWSAALHQVQAMRAQKLSGGFAARLQKPELDRTRLPVIL
                     PRAGAAISAAKAKLLSFGDAYALNLPQDRGVQITVYGNRTFVPADPGAISRRPMQRLS
                     GVAENVAVEQTEDGWTATFQRFGVVYTVDVSCDDIASPTCASDAYIRKTVADFSDVTL
                     GAQAQAEASAAAPPGLLSRFSQTIDKLVKGH"
     gene            754..>1545
                     /locus_tag="VF402_01360"
     CDS             754..>1545
                     /locus_tag="VF402_01360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7798965.1"
                     /translation="MRALYPLAALLLLAACDQTAPADTGDASSAAAAASEAESAASES
                     AVAQASTEAGAEADASALPDFPYAPPGELVANSGSGYSDATNWAPGICFPLAQTPDYA
                     NSQVYGPGGDHYPSTSATNHGSQCDGANYAYPWHDTFCEARSWTNPVCKSGSGHQGQD
                     IRPTTCKKDAYWAVAAEDGVITQVGSYTVAVTGNASPHRIFRYLHMQSSSVLVKVGDH
                     VARGQKLGLVSNNFGVSNGVQQYTTIHLHFEVRVAQAETMADGTQL"
BASE COUNT          247 a          585 c          506 g          207 t
ORIGIN      
        1 gccggacagc gttaaaccgg atcgcggcgc ctatgcgccc gcgctgatcg gcgcgggcgc
       61 agccgccgtt ctgctggccg cggcggcctt cggggccgcg acctacgccc agagcctgaa
      121 caaggaccag aacgcggcgc gcgcccagcc cgtctttcgc ttgcgcaagc cgctgccgcc
      181 cgcccgcgtc gaatggtccg ccgccttgca tcaggttcag gccatgcgcg cgcagaagct
      241 cagcggcggg ttcgccgccc ggctgcaaaa accggaactg gaccgcacgc gcctgcccgt
      301 catcctgccg cgcgcgggcg ccgcaatcag cgcggccaag gcgaagctgc tgtcgttcgg
      361 cgacgcctat gcgctcaacc tgccgcagga ccggggcgtg cagatcacgg tgtacggcaa
      421 ccgaaccttc gtgcccgccg atccgggcgc gatctcgcgc cgcccgatgc agcggctgag
      481 cggcgtggcc gagaacgtcg cggtcgagca gaccgaggac ggctggaccg ccaccttcca
      541 gcgtttcggc gtggtctata cggtcgacgt gtcctgcgac gacatcgcca gcccgacctg
      601 cgcgtccgac gcctatatcc gcaagacggt cgccgatttc agcgacgtga cgctgggcgc
      661 gcaggcgcag gccgaagcgt ccgccgccgc gccgcccggc ctgctgagcc ggttcagcca
      721 gacgatcgac aagctggtga aggggcattg aagatgcgcg cgctctatcc cctcgccgcc
      781 ctgctgctgc tcgccgcctg cgaccagacc gcgcccgccg ataccggcga cgcttccagc
      841 gccgccgccg cggccagcga ggccgagtcc gccgcctcgg aaagcgccgt cgcgcaagcc
      901 tcgaccgagg ccggcgccga ggcggacgcc tccgccctgc ccgactttcc ctacgcgccg
      961 cccggcgagc tggtggccaa ttccggttcg ggctacagcg acgcgaccaa ctgggcgccc
     1021 ggcatctgct tcccgctggc ccagacgccc gactacgcca acagccaggt gtacggcccc
     1081 ggcggcgacc attatcccag cacgtcggcc acgaaccacg gcagccagtg cgacggcgcc
     1141 aactacgcct atccgtggca cgacaccttc tgcgaggcgc gaagctggac caacccggtg
     1201 tgcaaaagcg gctccggcca tcaggggcag gacatccgcc cgaccacctg caagaaggat
     1261 gcgtactggg ccgtcgccgc cgaggacggc gtgatcaccc aggtcggctc ctatacggtg
     1321 gcggtgacgg gaaacgcctc gccgcaccgc atcttccgct acctgcacat gcagtcgtcg
     1381 tcggtgctgg tgaaggtcgg cgaccacgtc gcgcgcggcc agaagttggg gctcgtctcc
     1441 aacaatttcg gcgtctccaa cggcgtgcag cagtacacca ccatccacct gcatttcgag
     1501 gtgcgcgtgg cgcaggccga gaccatggcc gacggcacgc aactg
//
LOCUS       DASVLL010000081         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_4747, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000081 DASVLL010000000
VERSION     DASVLL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4747"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /gene="acs"
                     /locus_tag="VF402_01365"
     CDS             complement(<1..1861)
                     /gene="acs"
                     /locus_tag="VF402_01365"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX7798966.1"
                     /translation="MDDHATHPASAVFPVPQGFLRPGTLTAEGLQAQHDLFAKDPDAH
                     WRTLGDALDWSKPYTKVRDVSFDREDFRIRWFHDGQLNVSVNCIDRHLATRGDAVAFI
                     FEGDDPGVSYAVTYKQLSAEVNRRANLLKQYGVKKGDRVTIYLPMIPEAAFFMLACAR
                     IGAVHSVVFGGFSPDSIAGRILDCRSDIVITANEGVRGGKTIPLKANVDQALLRCPDV
                     RHVLVVEHTAAHYLTHGGRDVSVAKALEGVSDRCEPEPMNAEDPLFILYTSGSTGKPK
                     GVLHTTGGYLAWAAYTHKTVFDYQDGDIYWCTADVGWVTGHSYVVYGPLANGATSLIF
                     EGVPNYPDASRFWRVIDKHKVNIFYTAPTAIRALMREGEAPVKSASRASLRVLGSVGE
                     PINPEAWLWYHRVVGDERCPVVDTWWQTETGGILISPTPGATALKPGSATRPLPGVLP
                     CLVDAEGKVLDGATEGNLCLTESWPGQMRSVYGDHQRFLDTYFSTYPGKYFTGDGCRR
                     DEDGYYWITGRVDDVLNVSGHRIGTAEIESALVGHAGVTEAAVVGYPHDIKGQGIWCY
                     VTLKSGVTGTDALKKELRDHVRQEIGPIASPDVIQWAPGLPKTRSGKIMRRILR"
BASE COUNT          350 a          673 c          708 g          364 t
ORIGIN      
        1 tgcgcaggat gcggcgcatg atcttgccgg agcgcgtctt gggcaggccg ggcgcccact
       61 ggatcacgtc gggcgaggcg atcgggccga tctcctgacg cacatggtcg cgcagttcct
      121 ttttcagcgc gtccgttccg gtcacgcccg atttgagcgt gacatagcac cagatgccct
      181 gccccttgat gtcgtgcgga tagccgacca ccgccgcctc ggtcacgccc gcatggccga
      241 ccagcgcgct ttcgatctcc gccgtgccga tgcggtggcc ggacacgttc agcacgtcgt
      301 cgacgcggcc ggtgatccag taatagccgt cctcgtcgcg gcggcagccg tcgccggtga
      361 agtacttgcc cggataggtc gagaaatagg tgtcgaggaa gcgctgatgg tcgccataga
      421 cgctgcgcat ctggccgggc caggactcgg tcaggcacag attgccctcg gtcgcgccgt
      481 ccagcacctt gccctcggcg tcgaccaggc acggcagcac gccgggcagg ggccgggtgg
      541 ccgagccggg cttgagcgcg gtcgcgcccg gcgtcggcga gatcaggatg ccgcccgtct
      601 cggtctgcca ccaggtgtcc acgaccgggc agcgctcgtc gccgacgacg cggtggtacc
      661 acagccacgc ttcgggattg atcggctcgc cgaccgagcc cagcacgcgt aaagaggcgc
      721 gcgaggccga cttcaccggc gcctcgcctt cgcgcatcag cgcgcggatg gcggtcggag
      781 cggtgtagaa gatgttgacc ttgtgcttgt cgatcacccg ccagaagcgc gacgcgtccg
      841 ggtagttggg cacgccctcg aagatcagcg aggtcgcgcc gttggccagc ggcccataga
      901 cgacgtagga atggcccgtg acccagccga cgtcggcggt gcaccagtaa atgtcgccgt
      961 cctgatagtc gaagaccgtc ttgtgcgtat aggcggccca ggccagatag ccgcccgtgg
     1021 tgtgcaaaac gcccttcggc ttgccggtcg agccggaggt gtagaggatg aacaggggat
     1081 cctcggcgtt catcggctcc ggctcgcacc ggtcggacac gccttccagc gccttcgcca
     1141 ccgacacgtc gcgcccgccg tgggtcaggt aatgcgccgc cgtatgctcg accaccagca
     1201 cgtgacgcac atcggggcag cgcagcaggg cctgatcgac attggccttc agcgggatgg
     1261 tcttgccgcc gcgcacgcct tcgttggcgg tgatcacgat gtcggagcgg caatcgagga
     1321 tgcgcccggc gatgctgtcc ggcgaaaagc cgccgaacac caccgaatgc acggcgccga
     1381 tgcgcgcgca ggccagcatg aagaaagccg cttcggggat catcggcagg tagatggtga
     1441 cgcggtcgcc cttcttgacg ccgtactgtt tcagcaggtt ggcgcgccgg ttcacctcgg
     1501 cggacagttg tttataggtg acggcgtacg aaacgcccgg atcgtcgccc tcgaagatga
     1561 aggccacggc gtcgccgcgc gtcgccagat gccggtcgat gcagttgacc gagacgttga
     1621 gctggccgtc atggaaccag cggatgcgga aatcctcgcg gtcgaacgag acgtccctga
     1681 ccttggtgta gggcttgctc cagtccagcg cgtcgcccag cgtgcgccag tgcgcgtccg
     1741 ggtctttggc gaacagatcg tgctgcgcct gcaagccctc ggcggtcagc gtgccgggac
     1801 gcagaaagcc ctgcgggacc ggaaaaacgg cggaagcggg gtgcgtggcg tggtcatcca
     1861 ttttaatcct ccgggcgcgt cgattttgcg cggccaattc ttgttgcccc cagtctatat
     1921 aaacctgcgc gccggagtcg atatgaccaa tacgaaaaag tccttgtgac ggcgctgtca
     1981 tatggctttg cggcgcgatg cgcgcccatt gtgccgtaat aacattgttg acacgccgcg
     2041 ccgcggctct ataatcgttg cgatggagac gaagatcatg cgcgccgcat tgcgt
//
LOCUS       DASVLL010000082         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_380561,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000082 DASVLL010000000
VERSION     DASVLL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380561"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VF402_01370"
     CDS             complement(<1..923)
                     /locus_tag="VF402_01370"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX7798967.1"
                     /translation="MELSHPELLGLIPADHYWREDVLAEEMARVFAPSWLCVGFAEDL
                     KNHQDFVTAQFGPHSIVVQNFDGELRAFRNVCSHRFSRIQNAACGNRALQCPYHGWTY
                     GADGRLIGAPLNRQSFDLDDDDKRRLGLQAYAVETVGHFVFVRMSADGPSLRGFLGGL
                     YDDLAHVSEVCPDRFERAAYDWDANWKLGMDNAAEGYHVPLVHADSFGLILSIDLKIS
                     HDLEHTRYTGNLKERSLTWWRQVGKAIDLRPSEIYPQYGNFLIFPNIVVTFSYGAFLT
                     FQWFEPTGPESLRIHTTSWLAKNNGRAARGMT"
     gene            complement(923..>1550)
                     /locus_tag="VF402_01375"
     CDS             complement(923..>1550)
                     /locus_tag="VF402_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX7798968.1"
                     /translation="AGDVSTSKLLAGDRATIPLFGDAGSATALEIEPDAKDDWFGVFG
                     TDGSGARHIMVEAGGLKYPLLPPREPHSPEMAEDLYRKARLHLNGTEVFNFTLRAVPP
                     LVEAIFRTSGVTVEDIDYVLLHQANVFMLNHLRKKAGLPEDKAPIAMETYGNTSSASI
                     PLTIADRLSDVFDRPRQLVLMGFGVGWSWSALKLTAGPMPAPRVVELA"
BASE COUNT          293 a          513 c          485 g          259 t
ORIGIN      
        1 gtcatgcccc gcgccgcgcg gccattgttc ttcgccagcc acgaggtcgt atggatgcgc
       61 aggctttcag gccccgtcgg ctcgaaccac tgaaaggtca ggaacgcgcc gtaggaaaag
      121 gtcacgacga tgttcggaaa gatcaggaaa ttgccgtatt gcgggtaaat ctccgaaggc
      181 cggagatcga tcgccttgcc cacctgccgc caccatgtca gcgagcgctc cttcaggttt
      241 ccggtgtagc gcgtgtgctc cagatcgtgc gaaatcttca ggtcgatgga caggatcagg
      301 ccgaaactgt cggcatgcac caaaggcacg tgatagccct cggcggcgtt gtccatgccc
      361 agcttccagt tggcgtccca gtcataggcg gcgcgctcga aacggtcggg acagacctcg
      421 gagacatgcg ccagatcgtc gtacaggccg ccgagaaagc cgcgcaagga cgggccgtcc
      481 gcgctcatgc gcacgaagac gaaatgcccg accgtctcga cggcgtaagc ttgcaaaccc
      541 agccgcctct tgtcgtcgtc gtcaagatcg aagctctgcc ggttcagcgg cgcgccgatc
      601 agccgcccgt ccgcgccata ggtccagccg tgatacggac attgcagcgc gcggttgccg
      661 caggccgcgt tctggatgcg cgaaaagcgg tgcgagcaga cattgcggaa ggcgcgcaat
      721 tcgccgtcga aattctgcac cacgatgctg tgcgggccga actgcgcggt gacgaaatcc
      781 tgatggtttt tcaggtcttc ggcaaagccg acgcacagcc aggacggcgc gaacacgcgc
      841 gccatctcct cggccagcac gtcctcgcgc cagtaatgat ccgccgggat caggccgagc
      901 aattcgggat gcgacagctc catcacgcca gctcgacgac cctgggcgcg ggcatcggcc
      961 cggcggtcag cttgagcgcg ctccacgacc agccgacgcc gaagcccatc agcaccagtt
     1021 gccgcggccg gtcgaaaacg tccgacaatc tgtccgcgat ggtcagcggg atcgaggccg
     1081 acgaggtgtt gccataggtc tccatcgcga tcggcgcctt gtcctcaggc aggcccgcct
     1141 ttttgcgcag gtgattgagc atgaagacat tggcctgatg cagcagcaca tagtcgatat
     1201 cctcgaccgt gacgcccgac gtcctgaaaa tcgcctcgac cagcggcggc acggcgcgca
     1261 gcgtgaaatt gaacacctcg gtcccgttca gatgcagccg cgccttgcgg tataagtctt
     1321 ccgccatttc cggcgagtgc ggttccctgg gcggcaagag cggatatttg agcccgcccg
     1381 cctcgaccat gatgtgccgc gcgccggagc cgtcggtgcc gaacacgccg aaccagtcgt
     1441 ccttcgcgtc cggctcgatc tccagcgccg tggccgaacc ggcgtcgccg aacagcggga
     1501 tcgtggcgcg gtcgcccgcc agcagcttgg aggtcgaaac atcgcccgcc
//
LOCUS       DASVLL010000083         6282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_273939,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000083 DASVLL010000000
VERSION     DASVLL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273939"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF402_01380"
     CDS             complement(<1..239)
                     /locus_tag="VF402_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7798969.1"
                     /translation="MTQAAEPTSPAAARKPQWRDLALRAASAIVLIPAVLWIVVLGDG
                     LFLAMISVGVALLCIEWGMMAAPESSARVACAVAFA"
     gene            complement(236..1018)
                     /gene="uppS"
                     /locus_tag="VF402_01385"
     CDS             complement(236..1018)
                     /gene="uppS"
                     /locus_tag="VF402_01385"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX7798970.1"
                     /translation="MPSGPTEALVSAPATETKTCAPSKALHVAIIMDGNGRWAKERGQ
                     VRTLGHRAGVQALRRTITAAVELGVTHMTVFAFSTENWRRPQAEVSDLMGLLRAFIRS
                     DLDKLRREGVCVRILGQKEGLPADIVGMVEDAHEKTKDNSRFFLQVALNYGAQADIAA
                     AARRMAEEVAAGRMAPEDITPDSFGGFLSTRDLPPLDLLIRTSGEHRLSNFLLWEAAY
                     AEFVFQDILWPDYGRDALKAALDVFARRDRRYGGVQAAQDGA"
     gene            complement(1057..1614)
                     /gene="frr"
                     /locus_tag="VF402_01390"
     CDS             complement(1057..1614)
                     /gene="frr"
                     /locus_tag="VF402_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7798971.1"
                     /translation="MPLPDLHTYRDRMDKAVAALKDDFGGLRTGRASASLLDQVTVDA
                     YGSTMALNACAAISVPEPRMISVNVWDKGLVVSVEKAIRNAGLGLNPITDGQTLRIPI
                     PPLTEERRKELAKIAGKYAETQRVAVRNVRREAMEDLKRLEKASEISQDEEKRGGTEI
                     QGFTDAAIKRIDEVLKVKEAEIMQV"
     gene            complement(1630..2370)
                     /gene="pyrH"
                     /locus_tag="VF402_01395"
     CDS             complement(1630..2370)
                     /gene="pyrH"
                     /locus_tag="VF402_01395"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7798972.1"
                     /translation="MTSDSLKPAPGLKYKRILLKISGEVLMGEQGFGIDMKTVQAVAE
                     EVADVARLGVELCLVIGGGNIFRGLSKAALGMERSSADYMGMLATVMNALAMQNALEK
                     QGVDTRVQSAIQMNAVCEPFIRRRAIRHLEKGRVVIFAAGTGAPYFTTDTAAALRAAE
                     MNCDALFKGTSVDGVYTADPKKDASAERYDNLKYLEVLSRDLRVMDASAVSLMRENEI
                     PIVVFSIRTPGALNEVIHGRGTHTVITN"
     gene            complement(2501..3413)
                     /gene="tsf"
                     /locus_tag="VF402_01400"
                     /pseudo
     CDS             complement(2501..3413)
                     /gene="tsf"
                     /locus_tag="VF402_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919788.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
     gene            complement(3557..4339)
                     /gene="rpsB"
                     /locus_tag="VF402_01405"
     CDS             complement(3557..4339)
                     /gene="rpsB"
                     /locus_tag="VF402_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7798973.1"
                     /translation="MAMPEISMRLLLEAGAHFGHQTHRWNPKMERYLFGARSNIHIID
                     LSQTLPLFHQALLKVREVAASGGRVLFVGTKRQAQGSVATAAKRSAQYYVNHRWLGGT
                     LTNWRTISGSIQRLRELEQVLDNNPAGRSKKELLTLTREREKLELSLGGIKDMGGIPD
                     LMFVIDTNKEAIAIQEARKLNIPVIAILDSNSDPDGITYPVPGNDDAARALQLYCDLV
                     ADAVLDGLAAGQAASGVDLGAAIAPVEPALDAEVAAEAAGEA"
     gene            complement(4512..>6282)
                     /gene="dnaE"
                     /locus_tag="VF402_01410"
     CDS             complement(4512..>6282)
                     /gene="dnaE"
                     /locus_tag="VF402_01410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7798974.1"
                     /translation="GLVKFDFLGLKTLTTLKRAENYLRKRGVDINYSTLPLDDAPTYE
                     LLASGQSIGVFQLESQGMRDTLRKMRCNSLEEITALISLYRPGPMDNIDTYVDRKFGR
                     AEIDMLHPSLEPVLKETYGVIIYQEQVMQIAQILSGYSLGEADLLRRAMGKKKKEEMD
                     LQKARFISGAREKGVDEAQADSIFELVNKFAGYGFNKSHAAAYAFISYQTGYLKANYP
                     VEFFAATLSLDITNTDKLAVFYQDARRFGVTIRPPDINRSMADFDVEDGEVLYALGAI
                     RNVGFEAMQSVVQTREEGGKFTDLFDFLERIDPKAVNKRAIENLARAGAFDSIHPNRA
                     QIVAAADILIAYAQSIAADRAAAQVSLFGSNEASRPRLPKVAAASGPAQLDEELAAIG
                     FYLSGHPLEDMLDVFKRRNVTLYAEALSLAGEGREAFRMAGIVRRRQERAAAGSGEKF
                     AFVTLSDPSGEYEVLFPPESLRKNRDHLEPGGSIVLKVRSKGRDGEVRFFGDEAMPMS
                     EALKSEDMGLRVHVSAQIIDLADLRARIEASKSPRGGVISLAGDLAGEGEIEFRLPQR
                     YSLDANLRGQLKAAPGVLYFEDV"
BASE COUNT         1041 a         2103 c         1981 g         1157 t
ORIGIN      
        1 gcgaacgcca ccgcgcaggc gacccgcgcc gaggattccg gcgccgccat catgccccac
       61 tcgatgcaca gaagcgccac gccgacgctg atcatggcca ggaacaggcc gtcgcccagc
      121 acgacgatcc acaggaccgc cgggatcagc acgatggccg acgcggcgcg cagggccagg
      181 tcgcgccatt gcggcttgcg cgccgcggca ggagaggtcg gctccgcggc ttgcgtcatg
      241 cgccgtcctg cgccgcctgc acgccgccat agcgccggtc gcggcgcgcg aaaacgtcca
      301 gcgccgcctt cagcgcgtcg cgcccgtagt ccggccacag aatgtcctga aacacgaatt
      361 cggcgtaggc cgcttcccag agcaggaagt tggacaggcg atgctcgccg ctggtgcgga
      421 tcagcaggtc cagcggcggc aggtcgcgcg tcgacaaaaa gccgccgaaa ctgtccggcg
      481 tgatgtcctc tggcgccatg cgcccggccg cgacctcttc ggccatgcgc cgcgccgccg
      541 ccgcgatgtc ggcctgcgcg ccgtaattga gcgccacctg caaaaagaag cgcgaattgt
      601 ccttggtctt ttcgtgcgcg tcctcgacca tgccgacgat atcggcgggc aggccttcct
      661 tctggcccag aatgcgcacg cacacgcctt ccctgcgcag cttgtccaga tcggagcgga
      721 taaaggcgcg caacagcccc atcaggtcgc tgacctcggc ctgcggacgg cgccagttct
      781 cggtcgaaaa ggcgaagacc gtcatgtgcg tcacgcccag ttcgaccgcc gccgtgatgg
      841 tgcggcgcag cgcctgcacc ccggcgcggt ggcccagcgt gcggacctgc ccgcgctcct
      901 tggcccagcg cccgttgccg tccatgatga tggcgacgtg cagggccttc gacggcgcac
      961 aggtcttcgt ttccgtcgcc ggggcgctga ccagggcttc cgtcgggcct gagggcatga
     1021 acgcgtcttt cttacggggg agaggccggc ggacgcctag acctgcatga tctcggcttc
     1081 cttgaccttc agcacttcat cgatgcgctt gatggccgcg tcggtaaagc cctgaatctc
     1141 ggttccgccg cgcttttcct cgtcctggct gatctccgac gccttttcca gcctcttgag
     1201 atcctccatc gcctcgcggc gcacattgcg cacggcgacg cgctgcgtct cggcgtactt
     1261 gcccgcgatc ttggccagct ccttgcggcg ctcctcggtc aggggcggaa tcggaatgcg
     1321 cagggtctgg ccgtcggtga tcgggttcag gccgaggccc gcgttgcgga tcgccttttc
     1381 gaccgagacg accagtccct tgtcccagac gttgacgctg atcatgcgcg gctccggcac
     1441 gctgatcgcc gcgcaggcgt tgagcgccat ggtcgagccg taggcgtcga ccgtcacctg
     1501 atcgagcagc gaagccgagg cgcggccggt gcgcaaaccg ccgaaatcgt ccttgagcgc
     1561 ggcgaccgcc ttgtccatgc ggtcgcgata ggtgtgaaga tcaggcaggg gcatgggctt
     1621 tccctcttgt tagttcgtaa tgacggtatg ggtgccgcga ccgtggatga cctcgttcag
     1681 cgcgcccggc gtgcggatcg agaagaccac gatcggaatt tcgttctcgc gcatcagcga
     1741 caccgccgag gcgtccatca cgcgcaggtc gcgcgacagg acttcgagat atttcagatt
     1801 gtcgtaacgc tcggccgaag cgtccttctt cgggtcggcg gtatagaccc cgtccaccga
     1861 cgtgcccttg aacaaagcgt cgcagttcat ttcggcggcg cgcagcgcgg cggcggtgtc
     1921 ggtcgtgaag tacggcgcgc cggtcccggc ggcgaagatc accacgcggc ccttttccag
     1981 atggcggatg gcgcggcggc ggatgaacgg ctcgcacacg gcgttcatct gaatcgcgga
     2041 ctgcacgcgc gtgtcgacgc cctgcttttc cagcgcgttc tgcatggcca gcgcgttcat
     2101 gacggtggcc agcatgccca tatagtcggc ggacgaacgc tccatgccca gcgccgcctt
     2161 cgacaggccg cgaaagatgt tgccgccgcc gatgacgagg cacagctcga cgccgagccg
     2221 cgcgacgtcc gcgacctcct cggccacggc ctgcaccgtc ttcatgtcga tgccgaagcc
     2281 ctgctcgccc atcagcacct cgccggaaat cttcagcagg atgcgtttgt atttcagtcc
     2341 cggcgcgggt tttagcgagt cggacgtcat gtcgggttcc gggtctttga atcgttgcca
     2401 aggctttagc gcgttcgccg cgccttgcat agtcaaaggg gcgccggcat gtcaaacgcc
     2461 gacgcccctt atcccgttta ttttatcgac aaacgcgcga tcaggcgccc gcggtcatcg
     2521 aggcgacttc ggcggcgaaa tcgtccacct tcttctcgac gccttcgccc agggccagac
     2581 gggtgaagcc cttcagcgtc accggcgcgc ccagcgcctt ggcggtgtcg gccaccagtt
     2641 gcttgaccgt ctggtccggg ttcatcacga acggctgctc cagcagcacg acttcgcgct
     2701 ggaacttgga gatctggccg tcgacgatct tggcgatcat cgcttccggc ttgccttctt
     2761 ccttggcctt ttcggtcagg accgagcgct cgcgctcgac cgccgccgga tcgaggtcgt
     2821 cggacgacag cgacagcggg ctggtggccg cgatgtgcat ggcgatcttg cggcccatat
     2881 ccttcagcgc ttcctgatcg cccgccgatt cgacggcgac cagcaccgcg atgcggccca
     2941 ggtccggcgc gatggcgttg tggatgtagg acgcgaccac gccctgatcg accgagtggc
     3001 gggccatgcg gcgcacgacc atgttttcgc cgatggtggc gatcaggttg gtcagctcgt
     3061 cgccgacggc ggcggcgacg ccctcgacgg tttcgttctt cagaccggcc cggccggcgt
     3121 tgcgcgcggc gttctggaac agctcgttac gcgacacgaa gtcggtttcg gcgttcacct
     3181 cgacggcggc ggcggtcatg cccgcgccgt tcgcttcgct ggcgacggcc accaggccct
     3241 cggcggcgac gcggtcggcc ttcttggcgg ccttggacag gcccttggtg cgcagccagt
     3301 ccatcgcggc ttcgatatcg ccgccgtttt cctgcagggc cttcttgcag tccatcatgc
     3361 ccgcgcccga cttttcgcag atccttaacc agggatgccg tgatttcagc catgaaatcc
     3421 tccaataaaa tcagaaagtc gcgacctcat agccgcgata gatgtcaatt tggcaacagc
     3481 ttgaagccga aaaacggcct caagctgtaa acgtccatga accggcgaaa cgcgccggct
     3541 gtcatttggg aagccttcag gcttcgccgg cggcctcagc ggcgacctcg gcgtccagcg
     3601 ccggttcgac cggcgcgatg gccgcgccca gatcgacgcc cgacgccgcc tgacccgcgg
     3661 ccagaccgtc cagcaccgcg tcggcgacca gatcgcagta cagttgcagc gcgcgcgccg
     3721 cgtcgtcgtt gcccggcacc ggataggtga tgccgtccgg gtcggagttc gaatcgagga
     3781 tggcgatcac cggaatgttc agcttgcgcg cttcctgaat cgcgatggct tccttgttgg
     3841 tgtcgatcac gaacatcagg tcgggaatgc cgcccatgtc cttgatgccg cccaggctca
     3901 gctccagctt ttcacgctcg cgcgtcaggg tcagcagctc cttcttggag cggcccgccg
     3961 gattgttgtc cagaacctgc tccagttcgc gcagacgctg gatcgaaccc gaaatggtgc
     4021 gccagttggt cagggtgccg cccagccagc ggtggttgac atagtattgc gccgaacgct
     4081 tggcggcggt cgcaaccgag ccctgcgcct gacgcttggt gccgacgaac agcacgcgac
     4141 cgccggacgc ggcgacttcg cgcaccttca gcagcgcctg atggaacagc ggcagagtct
     4201 gcgacaggtc gataatatgg atgttggagc gcgcgccgaa caggtaacgc tccatcttcg
     4261 ggttccagcg gtgggtctgg tgaccgaagt gcgcgccggc ttccagcagc agacgcatcg
     4321 agatttcagg catagccatg agtatttgtc cttcttccga taatcctccg cggatcggaa
     4381 agccgccgga acggcggcct cggaacgcag tctccccaag ggacacggcg cttatccgcg
     4441 tgcgagatga cgcgcaccta ttcacaattt caaaaaaaag caagcctcac acgtcaaaaa
     4501 acgacgcaaa cctaaacatc ttcaaagtat agcacgcccg gcgccgcctt gagctgaccg
     4561 cgcaggttcg cgtcgagact atagcgttgc ggcagacgga actcgatctc gccctcgccc
     4621 gccagatcgc ccgccagact gatgacgccg ccgcgcggcg atttcgacgc ctcgatgcgc
     4681 gcgcgcagat cggccagatc gataatctgt gccgagacat gcacgcgcag gcccatatcc
     4741 tcgctcttga gcgcctcgct catcggcatg gcctcgtcgc cgaagaagcg cacctcgccg
     4801 tcgcggccct tggagcgcac cttcagcacg atcgagccgc ccggttcgag atggtcgcga
     4861 ttcttgcgca aggattccgg cgggaacaac acctcgtatt cgccgctcgg gtcggacagg
     4921 gtgacgaagg cgaatttctc gcccgatccc gccgccgcgc gctcctgcct gcggcgcacg
     4981 atcccggcca tgcggaacgc ttcgcgcccc tcgcccgcca gcgacagcgc ctcggcgtag
     5041 agggtgacgt tgcggcgttt gaacacgtcg agcatgtcct ccagcggatg ccccgacagg
     5101 tagaagccga tcgccgccag ttcctcgtca agctgcgccg gaccggacgc cgccgccacc
     5161 ttgggcaggc gcgggcgcga cgcctcgttg gagccgaaca ggctgacctg cgcggcggcg
     5221 cggtcggcgg cgatgctctg ggcgtaggcg atcagtatgt ccgccgccgc gacgatctgg
     5281 gcgcggttgg ggtggatcga atcgaacgcc cccgccctgg cgagattttc gatcgcccgc
     5341 ttgttgaccg ccttgggatc gatgcgttcg aggaaatcga acaggtcggt gaacttgccg
     5401 ccctcctcgc gcgtctgcac cacgctctgc atggcctcga agccgacatt gcggatggcg
     5461 cccagcgcat acagcacctc gccgtcctcg acgtcgaaat cggccatcga gcggttgatg
     5521 tccggcgggc ggatggtgac gccgaagcgg cgcgcatcct gataaaacac cgccagtttg
     5581 tcggtgttgg tgatatcgag actgagcgtc gcggcgaaga actcgaccgg atagttcgct
     5641 ttcagatagc ctgtctggta ggaaataaag gcgtaggcgg cggcgtgcga cttgttgaag
     5701 ccgtaacccg cgaatttgtt gaccagctcg aaaatgctgt cggcctgcgc ttcatcgacg
     5761 cccttttccc tggcgccgga gatgaaccgc gccttttgca ggtccatctc ctccttcttc
     5821 ttcttaccca tggcgcggcg caaaaggtcg gcttcgccca ggctgtatcc cgacaggatc
     5881 tgggcgatct gcatcacctg ttcctgatag ataatgacgc cgtaggtctc cttcagcacc
     5941 ggctccagcg acggatgcag catgtcgatc tcggcccgcc cgaacttgcg gtcgacatag
     6001 gtgtcgatat tgtccatcgg acccggacgg tagagcgaga tcagcgcggt gatttcctcc
     6061 agcgagttgc agcgcatctt gcgcagcgtg tcgcgcatgc cctgactttc aagctggaac
     6121 accccgatcg actgccccga ggccagcaat tcataggtcg gcgcgtcgtc cagcggcagg
     6181 gtcgaatagt tgatgtcgac gccgcgcttg cgcagatagt tttccgcccg tttcagcgtg
     6241 gtcagggtct tgaggccgag gaagtcgaac ttgaccagac cg
//
LOCUS       DASVLL010000084         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_219162,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000084 DASVLL010000000
VERSION     DASVLL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219162"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2574)
                     /locus_tag="VF402_01415"
     CDS             complement(<1..2574)
                     /locus_tag="VF402_01415"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX7798975.1"
                     /translation="MADALFSSLLDRLHPCGPVLAGSNSDFVFDMLYEAAKAEGWRDV
                     LTQAEPALRPVAAASPYLSGLMRRDPQRLRETLITAPEARLKAILLAVEAMGRQALTV
                     ETPDMAAARKILRHLKAECHLLTALADLGGVWSLDQVTAALSRFADAVTCAALALIAR
                     EERDRGRLRPDADLSGERGVLPGLFVLAMGKHGAGELNYSSDIDITFFADMERLPVAE
                     GVDPHSFADRVARGVSTLLSERTADGYVFRVDLRLRPDPSSTPTVVSVPFALNYYESV
                     GQNWERAAFIKARPVAGDMIEAQGFMQDLTPFVWRRSLDYPAIADVHSIKRQIHVYKV
                     DDRLDAAGANLKLGAGGIREIEFFAQTQQLILGGRDPSLRSRRTLDALDGLRRAGHLA
                     PAVAKELKQAYIRLRNWEHRVQMLNDEQTHELPENDEERLRVAALSGFVNLSRFDLAV
                     SRTLRLVNGHYGELFSEDEPLSSSFGSLVFTGIEDDPETLKTLKRMGFDHPEQVSATI
                     RSWHHGRIPATRSERGRELFTRLAPRLLEALHETGNPSIAFTRFAVFFATLNAGVQVQ
                     SLLLANPKLFRTVVEIMGFSPRLAQMLARYPTSFDAMLDAGFFDPLGEELDGIVAAEV
                     ARTPPELEPVMNALRRVGREQQFRIGMQILSGRLTTEAAGAAYARLADACLNHLAPLV
                     MDDMRRQAGDLGGQVAIIALGKLGSMEMTATSDLDLMTIYLPDDPSASSSLKGWAPET
                     FFGRFTQRLIAAVSAPTHEGRLYEIDLKLRPSGAKGPVAVSLAAFENYYTREADTWEF
                     LALTRARVVWASGADFADLARLKIETILRRQRNPAETALDVLAMRALMERERPAKGFW
                     DF"
     gene            complement(2905..>4026)
                     /locus_tag="VF402_01420"
     CDS             complement(2905..>4026)
                     /locus_tag="VF402_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7798976.1"
                     /translation="DAVIRQTLKTVTAANPTTMINYFSPSANDPQHCCRFQGRVLRLD
                     DSFYIYVAMDMSWTDHGFRQEVNAVIGGGLLVIVLGLFAGTLINQEVSRSVQRVTNVL
                     SQVSEGNLKARIAVRESGDEFDALSLRINQTLDRMEKTMGNLKYAGDAIAHDLRSPLS
                     RLRTRIEVSLLEVEKTGGDRVARDALAGALDETDQLLRTFQTVFAISRLQAAGQAPDQ
                     ALFSASDLAADFGELFEAVAADKGLDFKAEIEPGLSAYGNRDFLAQALANLLDNAIKY
                     TPAGGVTFRLRRTSQAEIEFSVTDTGPGVPEKDRVRITERFVRLENSRTLPGVGLGLS
                     MVAAVAAAHGGRFEVSEGPGVYDGTGPGLRTALALPPVG"
BASE COUNT          657 a         1415 c         1353 g          601 t
ORIGIN      
        1 gaagtcccag aaccccttgg ccgggcgctc ccgctccatc agcgcgcgca tggccagcac
       61 gtcgagcgcg gtttcggccg gattgcgctg acgccgcagg atggtctcga tcttcaggcg
      121 cgccagatcg gcgaaatcgg cgccggaggc ccacaccacc cgcgcgcgcg tcagggccag
      181 gaactcccag gtgtcggcct cgcgcgtgta atagttctcg aacgccgcca gcgacaccgc
      241 caccggcccc ttggcgccgg acggccgcag cttcaggtcg atctcgtaca gccgcccctc
      301 gtgcgtcggc gccgacaccg ccgcgatcag gcgctgggtg aagcgcccga aaaacgtctc
      361 cggcgcccag cccttgagcg acgacgacgc cgacggatcg tcgggcaggt aaatggtcat
      421 caggtcgagg tcggaggtcg ccgtcatctc catcgagccc agcttgccca gcgcgatgat
      481 cgcgacctgc ccgcccagat cgcccgcctg acggcgcatg tcgtccatga ccagcggcgc
      541 cagatggttc aggcacgcgt cggccagccg cgcataggcc gcgcccgccg cctcggtcgt
      601 caggcggccc gacaggatct gcatgccgat gcggaactgc tgctcgcgcc cgacgcggcg
      661 cagcgcgttc atcaccggct ccagctccgg cggcgtgcgc gcgacctcgg ccgcgacgat
      721 gccgtccagc tcctcgccca gcgggtcgaa gaaaccggcg tccagcatgg cgtcgaacga
      781 ggtcggatag cgcgccagca tctgcgccag acgcggcgag aagcccatga tctcgaccac
      841 ggtgcggaac agtttgggat tggccagcag cagcgactgc acctgcaccc cggcgttcag
      901 cgtggcgaag aacaccgcga agcgcgtgaa ggcgatcgac ggattgcccg tctcgtgcag
      961 cgcctccaga aggcgcggcg ccagccgcgt gaacagttcg cgcccgcgtt cggagcgcgt
     1021 cgccggaatg cgtccgtggt gccatgagcg tatggtggcc gacacctgtt cgggatggtc
     1081 gaagcccatg cgcttcagcg tcttgagcgt ttcgggatcg tcctcgatgc cggtgaagac
     1141 caggctgccg aacgaggacg acagcggctc gtcctcggaa aacagctcgc cgtaatggcc
     1201 gttgaccagc cgcagggtgc gcgacaccgc cagatcaaaa cgcgacaggt tgacgaagcc
     1261 cgacagcgcc gccacgcgca ggcgctcctc gtcgttttcg ggcagttcgt gcgtctgctc
     1321 gtcgttgagc atctgcacgc gatgctccca gttgcgcagg cggatatagg cctgtttcag
     1381 ctccttggcg acggcgggag ccagatggcc ggcgcggcgc agcccgtcga gcgcgtcgag
     1441 cgtgcgccgc gaccgcagcg acggatcgcg cccgccgagg ataagctgct gcgtctgggc
     1501 gaagaattcg atctcgcgga tgccgcccgc gccgagcttg agattggcgc ccgccgcgtc
     1561 cagccggtcg tcgaccttat agacgtgaat ctggcgcttg atcgaatgca cgtcggcgat
     1621 ggccggatag tcgagcgagc gccgccacac gaacggcgtc agatcctgca tgaagccctg
     1681 cgcctcgatc atgtcgcccg ccacgggccg cgccttgatg aaggcggcgc gctcccagtt
     1741 ctggccgacc gattcgtagt agttgagcgc gaacggcacg ctgaccacgg tcggcgtcga
     1801 ggacggatcg ggccgcaggc gcaggtccac gcggaacacg tagccgtccg ccgtgcgctc
     1861 ggacaacagc gtcgagacgc cgcgcgccac gcggtcggcg aagctgtgcg gatcgacgcc
     1921 ctcggccacc ggcagacgct ccatgtcggc gaagaaggtg atgtcgatgt cggaggaata
     1981 gttcagttcg cccgcgccgt gcttgcccat ggccagcacg aacaggccgg gcaggacgcc
     2041 gcgctcgccg ctcaggtcgg cgtcggggcg cagccgcccg cggtcgcgct cctcgcgcgc
     2101 gatcagggcc agcgccgcgc aggtcaccgc gtcggcgaag cgcgacaacg ccgccgtcac
     2161 ctgatccagc gaccacacgc cgccgagatc ggccagcgcg gtcagcaggt ggcattcggc
     2221 cttgagatgg cgcaggattt tccgcgccgc cgccatgtcc ggggtttcca cggtcagcgc
     2281 ctgccgcccc atcgcctcga ccgccagcag gatcgccttc agccgcgcct ccggggcggt
     2341 gatcagggtt tcgcgcaggc gctgcgggtc gcggcgcatc aggccggaca gatagggaga
     2401 agccgccgcc accggccgca gcgccggttc ggcctgggtc agcacgtcgc gccagccctc
     2461 ggccttcgcc gcctcgtaca gcatatcgaa cacgaaatcg ctgttcgacc ccgccagaac
     2521 ggggccgcac ggatgcagcc ggtcgagaag tgaggaaaac agtgcgtccg ccatgccgcg
     2581 cgtcagtctc cagggatcgg gtttctcact taaacccgac tcgacggaaa atccaaggac
     2641 gacgtaggct tactcccccc tacccctctc ccctccacga ggggagagga cgagagccga
     2701 gcgcagcgaa ggcgatgggc ggggcgcgca gcctgagggg ccgcccgccg accgcgctcc
     2761 gtcgtcgcca gcccctcacc cgaggatcgg cggcttcgcc ttgctcctcg tcctctcccc
     2821 tcatggaggg gagaggggga agaacaacgg cggcgccctt ccttttttga cgtagatccc
     2881 cttccccgtg aggggaagtg gagcctaccc taccggcggc agcgccagcg cggtgcgcag
     2941 gccggggccg gttccgtcat agacgcccgg cccttcgctg acctcgaagc gcccgccgtg
     3001 cgcggcggcg accgccgcga ccatgctgag gcccagcccc acgcccggca gggtgcggct
     3061 gttctccagc cgcacgaagc gttcggtgat gcggacgcgg tccttttccg gcacgccggg
     3121 gccggtgtcg gtgaccgaaa actcgatctc ggcctgcgag gttcggcgca ggcggaaggt
     3181 cacgccgccc gcaggcgtgt acttgatcgc gttgtcgagc aggttggcca gagcctgcgc
     3241 caggaaatcg cggttgccgt aggcgctcag ccccggctcg atctcggcct tgaagtccag
     3301 ccccttgtcg gcggcgaccg cctcgaacaa ttcgccgaaa tcggcggcca gatcgctggc
     3361 ggagaacagc gcctgatccg gcgcctgccc ggcggcctgc aaacgcgaga tcgcgaacac
     3421 ggtctggaag gtgcgcaaaa gctggtccgt ctcgtccagc gccccggcca gcgcatcgcg
     3481 cgcgacgcgg tcgccgccgg tcttttcgac ctccagcagc gacacctcga tgcgcgtgcg
     3541 caggcgcgac aagggcgagc gcaggtcgtg cgcgatggcg tcgcccgcgt atttcaggtt
     3601 gcccatcgtc ttttccatgc ggtcgagcgt ctgattgatg cgcaaggaca gcgcgtcgaa
     3661 ttcgtcgccc gattcgcgca cggcgatgcg cgccttgaga ttgccctcgc tgacctgcga
     3721 cagcacgttg gtgacgcgct gcaccgaacg cgacacctcc tgattgatca aggtgcccgc
     3781 gaacaggccc agcacgatga ccagaagccc gccgccgatc acggcgttga cctcctgccg
     3841 gaagccgtgg tcggtccacg acatgtccat cgcgacatag atgtagaagc tgtcgtccag
     3901 ccgcagcacc cggccctgaa agcggcagca atgctgcgga tcgttggccg acggcgagaa
     3961 atagttgatc atcgtggtcg gattggccgc cgtgacggtt ttcagcgtct gccggatcac
     4021 cgcgtc
//
LOCUS       DASVLL010000085         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_488062,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000085 DASVLL010000000
VERSION     DASVLL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488062"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /gene="dprA"
                     /locus_tag="VF402_01425"
     CDS             complement(<1..967)
                     /gene="dprA"
                     /locus_tag="VF402_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7798977.1"
                     /translation="MFDHIQPAQDTRERFARLRLARTPRVGPVNFAHLMQRFGSAMRA
                     LDELPGLMRRNGGSLTPPDPARVEAEIARGEAVGARLIVAGDDVYPALLAQADAAPPV
                     LWALGPLAVKRRCVAIVGARNASAAGQKMAQMLAHDLGRLGFQIVSGMARGVDARAHE
                     MSLATGAIAVLGGGVDDVYPPDNAGLYERLRAEGLILSESPVGHRAQAQDFPRRNRII
                     SGLSLATVVVEAELRSGSLITARLAAEQNREVFAVPGSPLDPRSRGANDLLRQGVNLC
                     ESADDVVRVLEGPFGVRAPEADYQPFATGPDLEAGIARAVRVLTDL"
     gene            1165..1338
                     /locus_tag="VF402_01430"
     CDS             1165..1338
                     /locus_tag="VF402_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798978.1"
                     /translation="MAFVGFGGAEAFVCFVAKKSCGHRPYGHFTHILIIVVVKRPYGH
                     FTTGGMDDMMDGT"
     gene            complement(1287..>1641)
                     /locus_tag="VF402_01435"
     CDS             complement(1287..>1641)
                     /locus_tag="VF402_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX7798979.1"
                     /translation="YYERFKAWCDDYFYLPHRDEPRGVGGVFYDHLDSGDHAADFAFT
                     QDVGKAFLAVYPELVRGRMNQAWTEAEREAQLIQRGRYVEFNLLYDRGTIFGLKTGGY
                     VPSIMSSMPPVVKWP"
BASE COUNT          263 a          524 c          592 g          262 t
ORIGIN      
        1 tcaggtccgt caggacgcgc acggcgcgcg cgatcccggc ttcgaggtcg ggaccggtgg
       61 cgaacggctg ataatcggct tccggcgcgc ggacgccgaa cgggccttcc agcacgcgca
      121 cgacgtcgtc cgcgctttcg cacagattga cgccctgccg taaaagatcg ttcgcgcccc
      181 ggctgcgcgg gtcgagcggc gagccgggga cggcgaagac ctcgcggttc tgttcggcgg
      241 cgaggcgggc ggtgatcagc gagccggagc gcagttcggc ctcgaccacg acggtggcca
      301 gggacaggcc ggagatgatg cggttgcggc gcggaaagtc ctgcgcctgc gcgcggtggc
      361 cgaccgggct ttccgacagg atcaggccct cggcgcgcag ccgctcgtaa agaccggcgt
      421 tgtccggcgg atagacgtcg tcgacgccgc cgcccaaaac ggcgatggcg ccggtggcga
      481 ggctcatctc gtgcgcgcgg gcgtcgacgc cgcgcgccat gcccgaaacg atctgaaacc
      541 cgagccgtcc gagatcgtgc gccagcatct gcgccatctt ctgcccggcg gcggaggcgt
      601 tgcgcgcgcc gacgatggcg acgcagcgcc gcttgacggc cagcggcccc agcgcccaca
      661 gcaccggcgg cgcggcgtcg gcctgcgcga gcagggccgg atagacgtcg tcgcccgcca
      721 cgatcagccg cgcgccgacg gcctcgccgc gcgcgatctc cgcctccacg cgcgccgggt
      781 cgggcggcgt caggctgccg ccgttgcggc gcatcaggcc gggcagttca tcgagcgcgc
      841 gcatggccga accgaagcgc tgcatcaggt gggcgaaatt gaccggcccg acgcgcggcg
      901 tacgcgccag ccgcagccgc gcgaagcgct cgcgcgtgtc ctgcgccggt tgtatgtgat
      961 cgaacatccc ccgtccctcc gtaggggagg ctaacagggt ttggcgcggc gggaaagcga
     1021 ggttcggctg ttttggccgg atggggcagg cggggggtag tttttttgcc acgaaaagca
     1081 cgaaaggaca cgaaaatcag gatcgcgtcc ttggggttat gtcttgcgaa tctgaatgat
     1141 aaagcgccgc ttcgaggcgt tcagatggct ttcgtgggct tcggcggcgc cgaagccttc
     1201 gtgtgtttcg tggctaaaaa atcttgtggt catcggcctt acggccactt cacccacatt
     1261 ttaattatag tcgtggtgaa gcggccttac ggccacttca ccacgggcgg catggatgac
     1321 atgatggacg gcacgtagcc gccggtcttg aggccgaaga tggtgccgcg gtcgtagagc
     1381 aggttgaact cgacatagcg gccgcgctgg atcagttgcg cctcgcgctc ggcttccgtc
     1441 cacgcttgat tcatgcggcc gcgcacaagt tcgggataga cggccaggaa cgccttgccg
     1501 acgtcctgcg tgaaggcgaa gtcggcggcg tggtcgccgg aatcgaggtg gtcgtagaag
     1561 acgccgccga cgccgcgcgg ctcgtcgcgg tggggcaggt agaaatagtc gtcgcaccac
     1621 gccttgaagc gctcgtaata g
//
LOCUS       DASVLL010000086         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_380758,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000086 DASVLL010000000
VERSION     DASVLL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380758"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VF402_01440"
     CDS             <1..810
                     /locus_tag="VF402_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX7798980.1"
                     /translation="EVQRVQLSNYAAPFAIMGNVYAPKYRQASLYTQLTLKQDAVEAR
                     QFAYGDIAAAFQRFLKTRRGGRGFVVVGLEQGGLLAERLIRDAVVPDPELKKQLVAAY
                     FIDTAAPESQFGLNGAILHACAARQEAGCVVSYISVDANRPDLALQMTQKAAKWQGDT
                     LTALGADKPVCVNPLLGAATTEPVDAKRSQGATNATGLEWGADPALVAHKVSAHCMGG
                     LLFIDKPGSPAFKSPASWEDRRKVRPYNLFYGDLQADIQARWQLYHAEPAA"
     gene            complement(807..2873)
                     /gene="recG"
                     /locus_tag="VF402_01445"
     CDS             complement(807..2873)
                     /gene="recG"
                     /locus_tag="VF402_01445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX7798981.1"
                     /translation="MRPEILFPYFANIASLKGVGPKLAPVLSQHVGPYVRDLVFFPPS
                     GVIRRPLTTLDHAQPGEVQTVAVTIGGYPPSGPKGPQRIETFDGGRRLSLVYFHRIRG
                     FETQHPVGARRLVSGKIESFNGALQMPHPDYIVSEDNAAEIPACEPVYPATADLSSRQ
                     IRRLAQTALGHVPALPEWLDESLLNKEIWPSFNEALQNLHAPAEPETLNVESPPRRRL
                     AYDEALAHQLALKARKSHRQASPARVIDAHGWADRAVDALPFALTGAQRRALDEVRAD
                     LRSGHRMNRLVQGDVGAGKTLVALLAMIDAAECGLQSVMMAPTEILARQHYEKCQPIL
                     DSLGVSSVIMTGRDKGRAREDKRAAVAAGDALIVFGTHAVFQEGVDFHALHLAVIDEQ
                     HRFGVGQRQRLFSKGEAVHYLSMSATPIPRTLALTQYGEADLSLLDEKPAGRQPIHTA
                     VLPRQRLNDVMARLSNAIRDGAQAYWICPLVEETAESDLIAVEARHRELQTALGMEIG
                     LVHGRMPSEDKDAVVARFAAGEVKILCATTVVEVGIDVPKASIIIIEQAERFGLAQLH
                     QLRGRVGRGADKSACILLYDTPLSKSAKARLELLRDTEDGFRIAEMDWRLRGEGDILG
                     ARQSGFPAYRFVDPVAHADLIATAAREAVYLLQHERRLPASRRAALDVLKNLFDWRAD
                     VADKQD"
     gene            2941..>3132
                     /locus_tag="VF402_01450"
     CDS             2941..>3132
                     /locus_tag="VF402_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX7798982.1"
                     /translation="MTSSEAREARLRKLSFRAWRRGFKEADIILGNFADDRLPGMTDA
                     QLDIFEVLLEVPDQDLYGWI"
BASE COUNT          513 a         1002 c         1072 g          545 t
ORIGIN      
        1 gaggtgcagc gtgtgcaact ctccaactat gcggcgccgt tcgccatcat gggcaacgtc
       61 tatgcgccga aatatcgtca ggccagcctc tatacgcaat tgacgctgaa gcaggatgcg
      121 gtcgaggcgc ggcagtttgc ctatggcgac atcgccgccg ccttccagcg ctttttaaag
      181 acgcggcgcg gcgggcgcgg ctttgtcgtc gtcgggctgg agcagggcgg tctgctggcc
      241 gaacgcctga tccgcgacgc ggtcgttccc gaccctgagt tgaaaaagca gcttgtcgcg
      301 gcctatttca tcgacacggc ggcgccggaa tcgcaattcg gcctcaacgg cgccatcctg
      361 cacgcctgcg ccgcgcggca ggaggccggt tgcgtcgtca gctacatctc ggtcgacgcc
      421 aaccggcccg acctggcctt gcagatgacg cagaaggcgg cgaaatggca gggcgacacg
      481 ctgaccgccc tgggcgcgga caagccggtc tgcgtcaatc cgctgctggg cgcggcgacg
      541 acggagccgg tcgacgccaa gcgttcgcag ggggcgacca atgcgacggg gctggaatgg
      601 ggcgccgatc cggccttggt cgcgcacaag gtgtcggcgc actgcatggg cggccttctg
      661 ttcattgaca agcccggatc gccggccttc aagtcgccgg ccagttggga ggatcgccga
      721 aaagtgcgtc cttacaatct gttctacggt gatcttcagg cggatattca ggcgcgctgg
      781 cagctttatc acgccgagcc cgcagcctaa tcctgcttat cggcgacatc ggcgcgccag
      841 tcgaacaggt ttttcagcac gtccagcgcg gcgcggcgcg atgcgggcag tcgccgttcg
      901 tgttgcagca gatagacggc ttcgcgcgcc gccgtcgcga tcaggtcggc gtgcgcgacc
      961 ggatcgacga agcgataggc gggaaagccc gattggcgcg cgcccagaat gtcgccttcg
     1021 ccgcgcagcc gccagtccat ctccgcgatg cggaagccgt cctcggtgtc gcgcagcagc
     1081 tccagccgtg ctttcgcgct tttcgacagc ggcgtgtcgt agagcaggat gcaggcgctt
     1141 ttgtcggcgc cgcgcccgac ccggccgcgc aactgatgca gttgcgccag cccgaaacgc
     1201 tcggcctgtt cgatgatgat gatcgacgcc ttcggcacgt cgatgccgac ctcgaccacc
     1261 gtggtggcgc atagaatctt cacctcgccc gccgcgaaac gcgcgacgac cgcgtccttg
     1321 tcctcggacg gcatgcggcc gtgaacgagg ccgatctcca tgcccagcgc cgtctgcaat
     1381 tcgcggtgac gggcctcgac cgcgatcagg tcggattcgg ccgtctcctc gaccagcggg
     1441 cagatccagt aggcctgcgc gccgtcgcgg atggcgttgc tgagccgcgc catcacgtcg
     1501 ttcaggcgct ggcgcggcag gacggcggtg tggatcggct ggcgaccggc gggcttttcg
     1561 tcgagaaggc tcagatcggc ctcgccgtat tgcgtcaggg cgagggtgcg cgggatcggc
     1621 gtggcggaca tggacaggta gtggacggcc tcgcccttgg agaacaggcg ctgacgctgg
     1681 ccgacgccga agcggtgctg ctcgtcgatg acggccaggt gcagggcgtg gaaatcgacg
     1741 ccttcctgaa acaccgcatg ggtgccgaac acgatcagcg cgtcccccgc agcgacggcg
     1801 gcgcgcttgt cctcgcgcgc gcggcccttg tcgcggcctg tcatgatgac ggaggaaacg
     1861 ccgagagagt ccagaatcgg ttggcatttt tcgtaatgct gccgcgccag aatctcggtc
     1921 ggcgccatca tcacgctttg caggccgcat tcggcggcgt cgatcatggc cagcagcgcc
     1981 accagcgtct tgcccgcgcc gacatcgccc tgcaccagcc ggttcatgcg atggccggaa
     2041 cgcaggtcgg cgcgcacctc gtccagcgcg cgcctctgcg ccccggtcag cgcgaacggc
     2101 aacgcgtcga cggcgcggtc cgcccagccg tgagcgtcga tgacgcgcgc cgggctggcc
     2161 tgccggtgcg atttgcgcgc cttgagcgcc agttggtgcg ccagcgcctc atcgtaggcg
     2221 aggcggcggc gaggcgggct ttcgacgttc agcgtctccg gctcggcggg cgcgtgcagg
     2281 ttttgcagcg cttcgttaaa tgatggccat atctccttgt ttaaaagcga ttcatccagc
     2341 cattcgggca gggcgggaac gtggccgagc gcggtttgcg ccagccgccg gatctgccgc
     2401 gacgacaggt ccgccgtcgc cggataaacc ggctcgcagg ccggaatctc ggcggcgttg
     2461 tcctcgctga cgatatagtc gggatgcggc atttgcagcg cgccgttgaa cgattcgatc
     2521 ttgccgctga ccagacggcg cgcgccgacc ggatgctgcg tctcgaagcc gcgaatgcgg
     2581 tggaaataga cgaggctgag acggcgcccg ccgtcgaacg tctcgatgcg ttgcgggcct
     2641 ttcgggccgg acggcggata gccgccgatg gtgacggcca cggtctgcac ctcgccgggc
     2701 tgcgcatgat cgagcgtcgt cagcgggcgg cggatgacgc cggacggcgg gaaaaagacc
     2761 agatcgcgca cataggggcc gacatgctgg ctcagaacgg gcgccagctt ggggccgacg
     2821 cccttgaggc tggcgatgtt ggcgaagtag ggaaacaaaa tttctggccg catggggctt
     2881 atcaagcatc aaaattgctt tatgagaagc ccttgattat tgcggcaaag gcgaccagct
     2941 atgacctcat ccgaagctcg cgaagcgcgt ttgagaaaat tgtccttccg cgcctggcgg
     3001 cgcggtttca aggaggccga catcatcctc ggcaatttcg cggacgaccg cctgcccggc
     3061 atgacggacg cgcagctcga catcttcgag gttttgctgg aggttcccga tcaggatctc
     3121 tacggctgga tc
//
LOCUS       DASVLL010000087         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_380831,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000087 DASVLL010000000
VERSION     DASVLL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380831"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..848)
                     /gene="gluQRS"
                     /locus_tag="VF402_01455"
     CDS             complement(54..848)
                     /gene="gluQRS"
                     /locus_tag="VF402_01455"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX7798983.1"
                     /translation="MYRTRFAPSPTGYLHLGHAFSALTAYETARAHGGQFLLRVEDID
                     HTRCRPAFEQGIYDDLRWLGLKWPEPVLRQSAHLGDYRAALNRLREMGVLYADARTRK
                     GEAEAALSAPQGMDETAGGAAPAAWRLSLEACRRRLGARYDDLSFCEGAEERRADPGV
                     NGDVILGRKDIGVAYHLCVVVDDARQGISHVHRGRDLYDATHTQVLLQALLGLPTPVY
                     AHHALLLDDQGRRLAKRKGSKALRDYREDGLTPRDVRETITAAPKG"
     gene            1102..1662
                     /locus_tag="VF402_01460"
     CDS             1102..1662
                     /locus_tag="VF402_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7798984.1"
                     /translation="MQILKSPPSDWRALVLNADFRPLSYYPLSTRPWQEVVKAVFEGR
                     VDVVSTYDVEIRSPSMKMRLPSVVSLKHYIDQNRPPAFTRYNVFLRDRFCCQYCGARA
                     ELTFDHVVPVSQGGRSTWTNILTACAPCNLRKGGKTPRQAGMIPAQNPHRPTMYQLQE
                     LGRSFPPNYLHESWIDYLYWDTLLEA"
     gene            1686..1976
                     /locus_tag="VF402_01465"
     CDS             1686..1976
                     /locus_tag="VF402_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798985.1"
                     /translation="MTPVRFETEGFGVVRFAHTPPEPLRRALEAALNVRFEARRERLF
                     GPRVLSFVFMGERVKLKRLGDGGALIDLSEADDEMRETLIERLRQSPDFDSF"
BASE COUNT          418 a          851 c          770 g          417 t
ORIGIN      
        1 cgatccgttc tattcctgtc cgagactgca atactgggcg caaagctacg atctcacccc
       61 ttcggcgccg ccgtgatcgt ctcgcgcacg tcccgcggcg tcaggccgtc ctcacgatag
      121 tcgcgcagcg ccttcgagcc cttgcgcttg gccagccgcc gcccctgatc gtccagcagc
      181 agcgcgtgat gcgcgtaaac cggcgtcggc agccccagca gcgcctgcaa cagcacctgc
      241 gtgtgcgtgg cgtcgtaaag gtcgcgcccg cgatgcacgt ggctgatccc ctgccgcgcg
      301 tcgtcgacga ccacgcacag atgataggcg acgccgatgt ccttgcgccc cagaatcacg
      361 tcgccattga cgcccggatc ggcgcggcgt tcttcggcac cctcgcaaaa ggacagatcg
      421 tcgtaacgcg cgcccaaccg ccgccgacac gcctccagcg acagccgcca cgcggcgggc
      481 gcggcgccgc ctgccgtctc gtccatcccc tgcggcgcgc tcagcgcggc ctcggcctcg
      541 cccttgcgcg tgcgcgcgtc ggcgtacagc acgcccatct cgcgcaagcg gttcagcgcc
      601 gcgcgatagt cgcccagatg cgcgctctgg cgcagaaccg gctccggcca cttcagcccc
      661 agccagcgca ggtcgtcgta aatcccctgc tcgaacgccg ggcggcagcg cgtatggtcg
      721 atatcctcga cgcgcagcag gaactgcccg ccgtgcgccc gcgccgtctc atacgcggtc
      781 agcgcgctga aagcgtggcc gaggtgcagg taaccggtcg gggagggggc gaaacgggtg
      841 cgatacatgg ttatgggttt aaagcgtttc cccaaaaagt ggctccactt tttggataag
      901 gaagagcgac agaacaaaac ttagagcgtc gatctgatga aatcagatcg acgctctaac
      961 ggaagacggc gaaagtttca caaaaaattc ccattccctt tccgcagaat cgggttcact
     1021 ctttccacag gtcagaaccg cgtgcagaag gagatccgtc ttcaaaacgt gccgtttacc
     1081 gccgccttcc cggaggccgc catgcagatc ctgaaaagcc cgccctcgga ctggcgcgcg
     1141 ctggttctga acgccgactt ccggcctttg tcctattacc cgctgtcgac ccgtccgtgg
     1201 caggaggtcg tcaaggcggt gttcgaaggg cgcgtcgacg tggtctcgac ctacgatgtc
     1261 gaaatccgct cgccctcgat gaagatgcgc ctgcccagcg tggtgtcgct gaaacactat
     1321 atcgaccaga accggccgcc cgccttcacg cgctacaacg tcttcctgcg cgaccgcttc
     1381 tgctgccagt actgcggcgc gcgcgcggag ctgaccttcg accacgtcgt gccggtgtcg
     1441 cagggcgggc gcagcacctg gaccaacatc ctgaccgcct gcgcgccgtg caacctgcgc
     1501 aagggcggca agacgccccg gcaggcgggc atgatcccgg cgcaaaaccc gcaccgcccg
     1561 accatgtacc agcttcagga gctgggccgg tcgtttccgc cgaactatct gcacgaaagc
     1621 tggatagatt acctgtactg ggatacgctg ctggaagcgt gatcgcggga acggggggag
     1681 gcggcatgac gccggtgcgt tttgaaaccg agggcttcgg cgtggtgcgg ttcgcgcaca
     1741 cgccgccgga gccgctgcgg cgggcgctgg aggccgcgct gaacgtccgc ttcgaggccc
     1801 ggcgcgaacg cctgttcggc ccgcgcgtcc tgagcttcgt cttcatgggc gagcgcgtga
     1861 agctgaaaag gctgggcgat ggcggcgcgc tgatcgacct gtccgaagcc gacgacgaga
     1921 tgcgcgaaac cctgatcgaa cgcctgcgcc aaagccccga cttcgacagc ttctgatttt
     1981 ctttccctct cccctcatgg aggggagagg ggacgggtta gcgtcaaaac gagaggaggg
     2041 cgccgccatc cctcatgtcc ggtcgcgccg ttctttccct ctcccctcca cgaggggaga
     2101 ggacgagagg agcaaggcga agccgccgat cctcgggtga ggggctgtcg gacgtttgcg
     2161 ctcggtcgtc ggccagccct cacccgatcg ccttcgcctg acggctcggc tctcgtcctc
     2221 tcccctcatg gaggggagag gggacgggtt agcgtcaaaa cgagaggaga gcgccgccat
     2281 ccctcatgtc cggccgcgct gttctttccc tctcccctcc acgaggggag aggacgagga
     2341 tcaaggcgca gccgccgatc ctcgggtgag gggctgtcgg acgtttgcgc tcaaccggcg
     2401 gtcagcccct cacccgatcg ccatcgctgc gctcggctct cgtcctctcc cctcat
//
LOCUS       DASVLL010000088         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_166315,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000088 DASVLL010000000
VERSION     DASVLL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166315"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2716)
                     /locus_tag="VF402_01470"
     CDS             complement(<1..>2716)
                     /locus_tag="VF402_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HEX7798986.1"
                     /translation="GLIGYNQSAGVQNAYATGSVVSSGDSVGGLIGYNNNATLTDAYA
                     TGSIRGNHNVGGLIGFSLGSAYYNVYAMGSVTGGAYVGGLLGTSFGGVYFEDAFATGF
                     VRGQYTGGLVGVSESYTPTLIRSYYIPDTTGQCCTADGFGGGLAVTTAQLQGVLPSGF
                     SSTDWGTGPGLYPYLKVFFPNGAQAISGMAYADTSGDAAVGSVAVYSGGKQLGTSGVG
                     ANGYYYVVTDAGAIGATTGVGADLTLYGDANPSGVGYADTHALTDGNVTDLDVTTGRI
                     DVTTGAATDSSVQGGIASAFGSAIWSGVLANIGNPDYGLTATGAFTVDDDPPGFGNLT
                     IRSGGDLTVAHAITLNGNGSLTLDAHDNLNIDATVTAPGNNAVTLTYADGGAGDYGFG
                     LQSDGFQGSLDYTGSGGGLTINGAAYTLLYSLSDLAGISGGSGAYALARTLDASGATY
                     SASVVGQLSGALTGLGHTVANLTISGAGVDNTGLIGQLKSGGVVRDIGLAGGAITGGT
                     YVGGLVGVNDQGAVTDAYVTASVTGDALVGGLVGFNQSGIISNVIGAGSVSANGSGAG
                     GVAGQNSDQISRARATGAVTSIGGVAGGLAGVNNGAISVSFATGAVTSLGGGAAGGLV
                     GYDYGTISDAFATGAVTSLGGVAGGLAGGVTYGAISNAYATGYVRGATTAGVVGSSNG
                     STLSNLYYDSGTTGQSDVAATGMATAQMQSGGLPSGFDPAVWATGAGLYPYLKSAFPN
                     GVQAISGMATDLAGAPVAGAGVTLYAGGAVLATAGSGANGYYYVARSPGAVTASTLLG
                     ATLTLDGAAHASGLTFSDGRTLCGCNVVGVDIASQVADITSGAATYSGLQSQLGATFG
                     GAWSDLSANLGTSVYMLSLDNAFTFDQAISLDGTLLVTDNGGVTLDHDI"
BASE COUNT          431 a          935 c          925 g          425 t
ORIGIN      
        1 cgatgtcatg atccagcgtg acgccgccat tgtcggtcac cagcagcgtt ccgtcgaggc
       61 tgatcgcctg atcgaaggtg aaggcgttgt cgagcgacag catgtaaacg cttgtgccga
      121 ggttcgcgct gaggtcgctc caggccccgc cgaaggtcgc gccgagctgg ctttgcaggc
      181 cgctataggt cgcggccccc gacgtgatgt cggccacctg cgacgcgata tcgacgccga
      241 cgacgttgca gccgcacagg gtccggccgt cgctgaaggt caggccggag gcgtgggcgg
      301 cgccgtcaag cgtcagcgtc gcccccagca gcgtcgaggc ggtgaccgcg cccggcgacc
      361 gggcgacata ataatagccg ttggcgcccg aaccggccgt cgccagaacc gcgccgcccg
      421 catacagggt gacgccggcg ccggcgaccg gcgcgcccgc aaggtcggtg gccatgccgc
      481 tgatcgcctg cacgccgttg gggaaggcgc ttttcaggta gggataaagt cccgcgcccg
      541 tcgcccatac ggccggatcg aaaccggacg gcagcccgcc cgattgcatc tgcgccgtgg
      601 ccatgccggt agcggcgacg tcgctctgac ccgtcgtgcc gctgtcgtaa tagaggttgc
      661 tcaaggtgga gccgttgctg gacccgacca cgcccgcggt cgtcgcgccg cgaacatagc
      721 cggtggcgta ggcgttgctg atcgcgccgt acgtcacgcc cccggccagc ccgcccgcta
      781 cgccgccgag cgaggtgacc gcgccggtgg cgaaggcgtc gctgatcgtg ccgtaatcgt
      841 atcctaccag cccgcccgct gcgccgccgc cgagcgaggt gaccgcgccg gtggcgaagg
      901 agacgctgat cgcgccgtta ttgacgcccg ccagcccacc cgccacgccg ccgatcgaag
      961 tgaccgcgcc ggtggcgcgc gcacggctga tctggtcgga attctgcccg gctacgccgc
     1021 cggcgccgga gccgttcgcg ctgaccgaac cggcgccgat gacgttgctg ataatgccgg
     1081 actggttaaa cccgaccaga ccgccgacca gagcgtcgcc ggtcacggat gcggtgacgt
     1141 aggcgtcggt cacggcgcct tgatcgttta cgccgaccag tccgccgaca taggttccgc
     1201 cggtgatcgc tccgcccgcc aggccgatat cgcgcacgac gccgcctgat ttgagctggc
     1261 cgatcaggcc ggtattgtcg acgcccgccc cgctgatggt caggttggcg accgtatggc
     1321 cgaggccggt cagcgcgccg ctcaactggc ccaccacgga cgcgctgtag gtcgcgccgg
     1381 acgcgtcgag cgtccgcgcc agggcgtagg cgcccgaccc gccgctgatc cccgccagat
     1441 cgctcaggga atagagcagc gtataggcgg cgccgttgat ggtcaggccg ccgcccgaac
     1501 cggtatagtc gagcgatccc tgaaagccat ccgattgcag gccgaagccg taatcgcccg
     1561 cgccgccgtc ggcataggtc agcgtcaccg cattgttgcc cggcgccgtc accgtggcgt
     1621 cgatgttcag attgtcgtgg gcgtccagcg tcaggctgcc gttaccgttc agcgtgatgg
     1681 cgtgggcgac ggtcaggtcg ccgccgctgc ggatcgtcag attgccgaac cccggcggat
     1741 cgtcgtccac ggtgaaggcg cccgtggccg tgaggccgta gtcgggattg ccgatattgg
     1801 ccaggacgcc gctccagatg gcgctgccga aagccgaggc gatcccgccc tgcacgctgc
     1861 tgtcggtcgc cgcgccggtc gtgacgtcga tccggccggt cgtgacgtcg agatccgtga
     1921 cattgccgtc cgtcagggca tgcgtgtcgg catagccgac gccggaggga ttggcgtcgc
     1981 cgtacagggt caggtcggcg cccacgccgg tcgtcgcgcc gatcgcgccc gcgtcggtca
     2041 cgacgtagta atagccgttg gcgccgacgc ccgatgtccc cagttgcttg ccgccgctat
     2101 agacggcgac gctgccgaca gccgcgtcgc ccgacgtgtc ggcgtaggcc atgccgctga
     2161 tcgcctgcgc gccgttgggg aagaagacct tgagatacgg atagaggccc ggccccgtgc
     2221 cccagtcggt cgagctgaag cccgacggca ggacgccctg caactgcgcc gtggtcacgg
     2281 ccaagccgcc gccgaagccg tcggcggtgc agcattgccc ggtcgtgtcg gggatgtagt
     2341 agcttctgat cagggtgggc gtgtacgact ccgatacgcc gaccaggccg cccgtatatt
     2401 gaccgcgcac aaagcccgtg gcgaaggcgt cttcaaaata gacgcccccg aacgacgttc
     2461 ccagcagacc cccgacatag gcgccgccgg tcaccgaacc catggcgtaa acattgtagt
     2521 acgcactgcc gagactaaaa ccgatcagcc ccccgacatt gtggttgccc cggatcgaac
     2581 cggtggcgta ggcgtcggtg agcgtggcgt tattgttata tccgatcaat ccgccgacag
     2641 aatcgccgct gctgacgacc gaaccggtgg cgtaggcgtt ctggacgcca gcggactgat
     2701 tgtagccgat gagacc
//
LOCUS       DASVLL010000089         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_112506,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000089 DASVLL010000000
VERSION     DASVLL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112506"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VF402_01475"
     CDS             <1..854
                     /locus_tag="VF402_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HEX7798987.1"
                     /translation="WRAPGETLAQLRMGLRLGVKPRLCVTTTPKPVAALKALMAEAGT
                     VVTRSATRANLAGLADDFLSGLTALYGGTRLAAQELDGMVVEDEAAALWRAEDLARCY
                     GARPPRLDALVTAVDPPATSHGDACGIVVAGRLDGRGYVLCDATVSGLSPLGWARRVA
                     AVVRDHGAQQVVAEANQGGEMVRALLAQAGCEAPIRLVHARDGKRRRAEPVAALYEQG
                     RVTHCPGDGGRFNALDEELMALGAGETGRSPAKSLAKSPDRADALVWALTALLVEGRA
                     EPRMRRL"
     gene            1087..>1775
                     /locus_tag="VF402_01480"
     CDS             1087..>1775
                     /locus_tag="VF402_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX7798988.1"
                     /translation="MFKNWFNRERKQSAAGAVTAINLVGRPVWTPRNYACLAAEGFGR
                     NAVAYRCIRMIAEACASVPLRVEFGGRRTPEHPLQTLIDRPNAEQGGADLREALYGGL
                     QTAGNAYVEAAFLDGEAAPFELWSLRSDRMKVIPNRRGWPDAYEYAANGARVRIGRDD
                     EGWLKVLHLKLWHPLDDWYGFSPLEAAAFSIDVHNASGAWNKALLDNAARPSGALVYG
                     AKASERLSDDQ"
BASE COUNT          246 a          514 c          727 g          288 t
ORIGIN      
        1 cctggcgcgc gccgggagag acgctggcgc agttgcgcat ggggttgcgg ctgggggtga
       61 agccgcggct gtgcgtgacc acgacgccga agccggtcgc ggcgctgaag gcgctgatgg
      121 cggaggcggg cacggtggtg acgcgctcgg ccacgcgcgc caatctggcc gggctggccg
      181 acgattttct gagcgggctg accgcgcttt acggcggcac aaggctggcg gcgcaggagc
      241 tggacgggat ggtggtcgag gacgaggccg ccgccctgtg gcgggccgag gatctggcgc
      301 gctgctacgg cgcgcggccg ccgcgtctcg acgcgctggt gacggcggtc gatccgcccg
      361 cgaccagcca cggcgacgcc tgcgggatcg tcgtggccgg gcggctggac gggcgcggct
      421 atgtgctgtg cgacgcgacg gtgagcggtt tgtcgccgct gggctgggcg cggcgcgtgg
      481 cggcggtcgt gcgcgaccac ggcgcgcagc aggtcgtggc cgaggccaat cagggcggcg
      541 agatggtgcg cgccctgctg gctcaggccg ggtgcgaggc gccgatccgg ttggtgcatg
      601 cgcgcgacgg caagcgccgg cgggccgagc cggtggcggc gctctatgag caggggcggg
      661 tgacgcactg tcccggcgac ggcgggcggt tcaacgcgct ggatgaggaa ttgatggcgc
      721 tgggggccgg ggagacgggc aggagtccgg ccaagagtct ggccaagagt cctgacaggg
      781 cggatgcgct ggtgtgggcg ttgacggcct tgctggtcga agggcgggcg gagccgagga
      841 tgcggcggtt atgaggtggg gagagtaaga aaccccacca ccacatctcg cttcgctcgt
      901 ccggtccccc ccgaaatcga agatttcagg gaggtataga cgtgtggcgg gcggttggcc
      961 cctcacccga tcgccttcgc ttcgcttggc tctcgtcctc tccccattgg catggggaga
     1021 ggggaaggtt tcgcgtttgc gatcgcccct cctgttgccc cggataggga tgagaaaggt
     1081 gttttgatgt tcaaaaactg gtttaaccgg gagcgcaagc agagcgcggc gggggcggtg
     1141 acggcgatca acctggtcgg caggccggtg tggacgccgc gcaattacgc ctgtctggcg
     1201 gcggaggggt tcgggcgcaa tgcggtcgcc tatcgctgta tccgcatgat cgccgaagcg
     1261 tgcgcctcgg tgccgctgcg ggtcgagttc ggcgggcggc ggacgccgga gcatccgttg
     1321 cagaccctga tcgaccggcc caacgccgag caaggcgggg cggatctgcg cgaggcgctc
     1381 tatggcggtt tgcagacggc gggcaacgcc tatgtcgagg cggcgtttct ggacggcgag
     1441 gcggcgccgt tcgagctgtg gagcctgcgt tcggaccgca tgaaggtgat cccgaaccgg
     1501 cgcggctggc cggacgccta tgaatatgcg gccaacggcg cgcgcgttcg gatcgggcgc
     1561 gacgacgagg gttggctgaa ggtgctgcat ctgaagctgt ggcatccgct cgatgactgg
     1621 tacggttttt cgccgctgga ggcggcggcg ttttccatcg acgtgcacaa tgcgtcgggg
     1681 gcgtggaaca aggcgctgct cgacaatgcg gcgcggcctt cgggggcgct ggtctatggc
     1741 gccaaggcgt ccgagcgtct gtcggacgat cagtt
//
LOCUS       DASVLL010000090         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_166632,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000090 DASVLL010000000
VERSION     DASVLL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166632"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF402_01485"
     CDS             <1..560
                     /locus_tag="VF402_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX7798989.1"
                     /translation="APKAWLFLPHKVDTDKCFRNVRAGSHEANVEGVIAGVVDAATAN
                     SDTLKELSDTPSGREKLARIKVLWSSQPLPKGVIEYRADLDPVIKEKLRSFFLSYGQG
                     SGPEAERQRANLRTFQWGPMQPADESYLLPERYLEAIVAQDEANQSGDPAKIAAAKAE
                     LDAVEKAQAAQAAKTAPQAPADASR"
     gene            560..1366
                     /gene="phnE"
                     /locus_tag="VF402_01490"
     CDS             560..1366
                     /gene="phnE"
                     /locus_tag="VF402_01490"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX7798990.1"
                     /translation="MDAPDAMTKLAPPTTGMAQRLANWLFVLVFVAFLAVSFRGAHIY
                     DLHKLVDNSANTGAYLSDYLHPDFTDWKRYVKAMWETLEIAIWGTFLSILLAVPISFL
                     CSRNIAPLWVVQPMRALIALLRAFPDIVLGTAFLVAVGLGPLAGVLALTLNNTGTLAK
                     LFSEAVEAIETAPVEGVRATGGTRLHEIVWGVIPQVGPLWTSFALYRFESNARSATVL
                     GLIGAGGIGQLLFDNLNSFAYQQVSAIALIIVVAVTLIDILSQQMRKRLI"
BASE COUNT          282 a          503 c          493 g          281 t
ORIGIN      
        1 tcgcgccgaa ggcgtggctg ttcctgccgc acaaggtcga cacggacaag tgcttccgca
       61 atgtgcgcgc cggttcgcac gaggccaatg tcgaaggcgt gatcgcgggc gtggtcgacg
      121 cggccaccgc caattccgac acgctcaaag aactgagcga caccccgtcg ggccgcgaga
      181 agctggccag gatcaaggtc ttgtggtcgt cgcagcccct gcccaagggc gtgatcgaat
      241 accgcgccga cctcgatccg gtgatcaagg agaagctgcg ctccttcttc ctcagctacg
      301 gtcagggcag cgggccggag gccgagcgcc agcgcgccaa cctgcggacc ttccagtggg
      361 ggccgatgca gcccgccgac gaatcctacc tgctgcccga acgctatctc gaagccatcg
      421 tggcgcagga cgaggcgaac cagtcgggcg atccggccaa gatcgccgcc gcgaaagccg
      481 agctggacgc ggtcgaaaag gcgcaggccg cgcaggcggc caaaaccgcg ccgcaagcgc
      541 ccgcggacgc gagccgttga tggacgcgcc cgacgccatg acgaaactgg cgccgccgac
      601 caccggcatg gcgcagcggc tggccaactg gctattcgtg ctggtcttcg tcgcctttct
      661 ggcggtgagc tttcgcggcg cgcacatcta cgacctgcat aagctggtcg acaattcggc
      721 caataccggc gcctatctga gcgattacct gcacccggac ttcaccgact ggaagcgcta
      781 cgtcaaggcg atgtgggaga cgctggagat cgccatctgg ggcaccttcc tgtcgatcct
      841 gctggccgtg ccgatcagct ttttgtgttc gcgcaacatc gcgcccttgt gggtagtgca
      901 gccgatgcgc gccctgatcg cgttgctgcg cgcctttccc gacatcgtgc tgggcacggc
      961 gtttctggtc gcggtgggct tagggccgct ggccggggtg ctggccctga cgctgaacaa
     1021 caccggcacg ctggccaagc tgttctccga ggcggtcgag gccatcgaga cggcgccggt
     1081 cgaaggcgtg cgcgccaccg gcggcacgcg cctgcacgaa atcgtctggg gcgtgatccc
     1141 gcaggtcggg ccgttgtgga cctcgttcgc gctctaccgc ttcgaatcga acgcgcgctc
     1201 ggcgaccgtg ctgggcctga tcggcgcggg cggcatcggc caattgctgt tcgacaatct
     1261 caacagcttc gcctaccagc aggtgtcggc catcgcgctg atcatcgtcg tggcggtgac
     1321 cctgatcgac atcctgtcgc agcagatgcg caagcggttg atctagcgtt ttccacaaaa
     1381 gcaactttgg aaaagtgtat ccgcagattc acacagatga acacagataa gagacggcga
     1441 tggttcgccg tatcgacccg cgtctgactg tcttcatccc tgttgatgaa caccgatcgt
     1501 tattatccag atgaaatctg tgttcatctg tggataaaaa atcacgccgc cttcttacg
//
LOCUS       DASVLL010000091         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_166655,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000091 DASVLL010000000
VERSION     DASVLL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166655"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="VF402_01495"
     CDS             complement(<1..1698)
                     /locus_tag="VF402_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX7798991.1"
                     /translation="MCLYPKRRLAALLSGAVLLFAPLAAQAEGALQARTGRGVIDFND
                     ALIANFKTRVAANAEARSLWAANKAEADRLLTVPTSKISLNPRGTNETLEPLILAWRM
                     TDDRRYEQKIYEILSALCDRPSWETDLPLTKRDPVWHSDLGMGMYGQDFGRAYSAIRD
                     VLTPDQRRKLVDGYVRGVVAPVRADWLDPATRIHTLDTMGHNWWAHIVFGMGLGLVAI
                     LPDEPRASAWLREVDAAGREWWSYGGSDLETKIATFDADGAYSESINYGELALGSYLQ
                     YRRAWIEGVTTPPAPAPLLPKALDFFIHNAYPASGTSLSVNFGDGNPEANGSPALATA
                     WALGEHRPDYLWYIRLYNEDGGKTDLHNVYGLPHFFLSLPLPSEPGRQGAAPRLPLDA
                     WYRGMGWATLRNGWDKDATMLAIRSGFTWNHNHADTGSFILFDQGQPLLIDSGNSSYA
                     APEYDAYYRQSVAHNVVTFNGEAEPKEDTYLGSQLPGKMPLMMDGAGIRYVLADATGP
                     TSAHFSRNFRHVLWIDDVILIIDDVKAREPGQFEWLIHTAGTAKAQDGALHVVNGGAS
                     "
     gene            complement(1727..2362)
                     /locus_tag="VF402_01500"
     CDS             complement(1727..2362)
                     /locus_tag="VF402_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX7798992.1"
                     /translation="MAPFSPLILRATAPQGVVAALNDAIDAIVASDGEAAARDWSRNL
                     AGNLKTEVRLTDRIRAMPEMCDFLYDTARAYAYRCENALLNYAHYDDTEDLKDRKLEI
                     RINEGWANDMVAGDFNPVHFHQGCLFSCVLFLRLPDAYEAEFQADKARQHTVGCLQFI
                     DGRTVVGGRNLFVVKPEVGAFYLWPAWMLHCVYPFRGAGVRRSVSVNLSLV"
BASE COUNT          408 a          802 c          769 g          421 t
ORIGIN      
        1 gctggcgccg ccattgacga catgcagcgc gccgtcctgc gccttcgcgg tcccggcggt
       61 gtggatcagc cattcgaact ggcccggttc gcgcgccttc acgtcgtcga tgatcaggat
      121 cacgtcgtcg atccataaca catgacggaa attgcgcgag aaatgagccg aggtcggccc
      181 cgtggcgtcg gccagcacgt atcggatacc cgccccgtcc atcatcagcg gcatctttcc
      241 cggcagttgc gagccgagat aggtatcctc cttcggctcg gcctcgccgt tgaaggtgac
      301 gacattgtgc gccacgcttt gccggtaata ggcgtcgtat tccggcgccg cataggagga
      361 attgccggaa tcgatcagca gcggctggcc ctgatcgaac aggatgaacg atcccgtatc
      421 ggcatgatta tggttccagg tgaagccgga ccggatcgcc agcatcgtgg cgtccttgtc
      481 ccagccgttg cgcaatgtcg cccagcccat gccgcgatac caggcgtcga gcggcagcct
      541 gggcgccgcg ccctgcctgc ccggttcgct gggcaggggc aggctcagga agaaatgcgg
      601 caggccatag acattgtgca ggtcggtctt gccgccgtct tcgttataga ggcggatgta
      661 ccacagatag tcgggacgat gctcgcccag cgcccaggcc gtcgccaggg cgggactgcc
      721 gttggcctcc gggttgccgt cgccgaaatt gaccgacagc gacgtccccg acgccggata
      781 ggcgttatgg atgaagaagt cgagcgcctt gggcagcagg ggcgcggggg cgggcggcgt
      841 cgtcacgcct tcgatccacg cgcggcggta ttgcagatag ctgcccagcg ccagctcgcc
      901 gtaattgatc gactcgctgt aggcgccgtc ggcgtcgaag gtcgcaatct tggtttcgag
      961 gtcactgccg ccatagctcc accattcgcg gcccgccgcg tcgacctcgc gcagccacgc
     1021 cgaggcgcgc ggctcgtccg gcaggatggc gaccagcccc agccccatgc cgaagacgat
     1081 atgcgcccac cagttatgcc ccatcgtgtc gagcgtatgg atgcgcgtcg ccgggtcgag
     1141 ccagtcggcc cgcaccggcg cgaccacgcc cctgacatag ccgtcgacca gcttgcggcg
     1201 ctgatcgggc gtcagcacat cgcggatggc gctgtaggcg cggccgaaat cctggccgta
     1261 catgcccatg cccagatccg aatgccagac cggatcgcgc ttcgtgagcg gcaggtcggt
     1321 ctcccagctc ggccggtcgc acagggcgct gaggatttcg tagatcttct gttcgtaacg
     1381 ccggtcgtcc gtcatccgcc aggccaggat cagcggctcc agagtttcgt tcgtcccgcg
     1441 cggattgagg ctgatcttgc tggtcggaac cgtcagcagc cggtcggcct cggccttgtt
     1501 cgccgcccac agcgacctcg cctcggcgtt ggcggcgacg cgcgtcttga aattcgcgat
     1561 cagcgcgtca ttaaaatcga tgacgccgcg cccggttctg gcctgcaacg ccccttcggc
     1621 ctgagcggcc aaaggggcga acagcagcac ggcgccgctc aacagcgcgg ccagacggcg
     1681 tttcggatag aggcacatgg taactcctga aagcttgaag tacgcgtcac accagggaca
     1741 ggttgacgct cacgctgcgc cgcacgcccg cgccccggaa cgggtagacg caatgcagca
     1801 tccatgccgg ccagagatag aaggcgccca cctccggctt gacgacgaac aggttgcggc
     1861 cgcccaccac ggtgcggccg tcgatgaatt gcaggcagcc gaccgtgtgc tggcgcgcct
     1921 tgtcggcctg aaactccgcc tcgtaagcgt cgggcaggcg caggaacaga acgcagctga
     1981 acaggcagcc ctgatggaaa tgcaccggat tgaagtcgcc cgcgaccata tcgttggccc
     2041 agccctcgtt gatgcggatt tcgagcttcc tgtccttcag gtcttcggtg tcgtcatagt
     2101 gcgcgtagtt cagcagcgcg ttttcgcagc gataggcata ggcgcgcgcc gtatcataca
     2161 gaaaatcgca catctccggc atggccctga tccggtcggt cagccgcacc tcggtcttca
     2221 gattgcccgc cagattccgc gaccagtcgc gcgccgccgc ctcgccgtcg ctggccacga
     2281 tggcgtcgat cgcgtcgttc agcgccgcca ccacgccctg cggcgccgtc gccctgagga
     2341 tcagagggct gaacggcgcc acggtgcgga tatcttaagc ctgcgtcatg gccgatcctt
//
LOCUS       DASVLL010000092         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_219695,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000092 DASVLL010000000
VERSION     DASVLL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219695"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..773
                     /locus_tag="VF402_01505"
     CDS             15..773
                     /locus_tag="VF402_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7798993.1"
                     /translation="MKILVPVKRVLDYNVKVRVKADQSGVDLANVKMSMNPFDEIAVE
                     EAVRLKEKGVATEVVAVSIGPAQARDTLITAMTIGADRSLLIQTDQDVEPLGVARLLK
                     AVAEAEKPDLIIMGKQAIDGDNNATGQMLAALLNWPQATFASKVVIENGEAPKEAMVT
                     REVDGGHQTLGVKLPAVITTDLRLNEPRYASLPNIMKAKKKPLETKSVADYGVDVAPR
                     LKVVKVTEPPKRSAGVKVADAAELVSKLKSAGVL"
     gene            775..1710
                     /locus_tag="VF402_01510"
     CDS             775..1710
                     /locus_tag="VF402_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX7798994.1"
                     /translation="MAVLVIADHDGAHLRDTTLKTLTAAQKLSGEIDVLVAGQGVAAV
                     AAAAAQAAGVRKVLTAESAELKADIAEDVAAVVVSIAGGYEVVAFAATASGKNVAPRV
                     AALLDAALIGDVIEVVDAHTFVRPIYAGNALETVQTSDGKIVLTVRTTAFAPVAAEGG
                     SAGVEAVAVPAAFAGARFISEEKVVSDRPELGSARVVVSGGRALGSKEEFDAVLNPLA
                     EKLNAAVGASRAAVDAGYAPNDYQVGQTGKVVAPDLYIAIGISGAIQHLAGMKDSKVI
                     VAINKDAEAPIFGVADYGLVGDYKTIVPELMKALG"
     gene            1722..2042
                     /gene="cutA"
                     /locus_tag="VF402_01515"
     CDS             1722..2042
                     /gene="cutA"
                     /locus_tag="VF402_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX7798995.1"
                     /translation="MDVSEIVTVSIACASDDAAQELARALVEAKLAACAQTAMIRSTY
                     RWRGAVEQAPEVLLTLKTLAAKLPALEALVKARHGYEVPEILAAPVVWASADYAAWLR
                     ESLA"
BASE COUNT          413 a          740 c          829 g          345 t
ORIGIN      
        1 aagaggtgac gaccatgaag atactcgtgc ccgtcaaacg ggtgttggac tataacgtca
       61 aggtgcgcgt caaggcggac cagtcgggcg tcgatctggc gaacgtcaag atgtcgatga
      121 acccgttcga cgagatcgcg gtcgaggaag ccgtgcgcct gaaggaaaag ggcgtggcga
      181 ccgaggtggt cgccgtctcc atcggaccgg cgcaggccag ggacacgctg atcaccgcca
      241 tgaccatcgg cgcggaccgc agcctgctga tccagaccga tcaggatgtc gagccgctgg
      301 gcgtggcccg gctgctcaag gcggtggccg aggcggaaaa gcccgacctg atcatcatgg
      361 gcaagcaggc gatcgacggc gacaacaacg ccacgggcca gatgctggcc gcgctgctca
      421 actggccgca ggcgaccttc gcgtcgaagg tggtgatcga gaacggcgaa gcgccaaaag
      481 aagcgatggt gacgcgcgag gtcgacggcg gccatcagac gctgggcgtc aagctgcccg
      541 ccgtgatcac cacggacctg cgcctgaacg agccgcgcta tgcgtccctg cccaacatca
      601 tgaaggccaa gaagaagccg ctggagacga agtccgtcgc cgattacggc gtcgatgtcg
      661 cgccgcgcct caaggtcgtc aaggtgacgg agccgccgaa gcgttcggcg ggggtcaagg
      721 tggccgacgc cgccgaactg gtgtccaagc tcaagtccgc gggagtgctg taagatggct
      781 gttctggtta tcgccgatca cgacggcgcg cacctgcgcg acacgacgct gaaaaccctc
      841 acggcggcgc aaaagctgtc gggcgagatc gacgtgctgg tcgcgggtca gggcgtcgcc
      901 gccgtggccg ccgccgccgc gcaggccgcg ggcgtgcgca aggtgctgac cgccgaaagc
      961 gccgaactga aggccgacat cgccgaggac gtcgccgccg tggtggtgtc gatcgcgggc
     1021 ggctatgagg ttgtcgcctt cgcggccacc gcgtcgggca agaacgtcgc gccgcgcgtc
     1081 gccgccctgc tcgacgccgc gctgatcggc gacgtgatcg aggtggtgga cgcccacacc
     1141 ttcgtgcgcc cgatctatgc gggcaacgcg ctggagacgg tgcagacctc ggacggcaag
     1201 atcgtgctga ccgtgcgcac cacggccttc gcgccggtcg ccgccgaggg cggttcggcg
     1261 ggcgtcgaag cggtggcggt tccggcggcc ttcgcgggcg cgcgcttcat ttccgaggaa
     1321 aaggtcgtgt cggaccgccc ggaactgggc tcggcgcgcg tcgtcgtgtc gggcggacgc
     1381 gcgctcggct cgaaggaaga attcgacgcg gtgctgaacc cgctggccga aaagctgaac
     1441 gcggcggtcg gcgcctcgcg cgcggcggtc gatgcgggct atgcgcccaa cgactatcag
     1501 gtcggccaga ccggcaaggt ggtcgcgccc gacctctata tcgccatcgg catttccggc
     1561 gcgattcagc atctggccgg gatgaaggac tccaaggtga tcgtcgcgat caacaaggac
     1621 gccgaggcgc cgatcttcgg cgtcgccgat tacggtctgg tcggcgatta caagaccatc
     1681 gtgccggagc tgatgaaggc gctcggctaa ggtcaagggc gatggacgtg agcgagatcg
     1741 tcaccgtgtc catcgcctgc gcgtccgacg acgcggcgca ggaactggcg cgcgcgctgg
     1801 tcgaggcgaa actggcggcc tgcgcgcaga cggcgatgat ccggtccacc tatcgctggc
     1861 gcggcgcggt cgaacaggcg ccggaggttt tgctgaccct caagacgctg gcggcgaagc
     1921 tgcccgcgct ggaggcgctg gtgaaggcgc ggcacggcta tgaggttccc gaaattctgg
     1981 ccgcgcccgt ggtctgggcc agcgccgatt acgccgcctg gctgcgcgaa agccttgcct
     2041 agtccccctc tccccatgac aatggggaga ggacgaggag caaggcgaag ccgccgatcc
     2101 tcgggtgagg ggctggcgac ggttgagcgc taacggcaac agcccctcac ccgatcgcct
     2161 tcgctgcgct cggctctcgt cctctcccct caatgagggg agagggatag tttaaggatt
     2221 agaggccacc ccatgaccga gattcacgga acctgccacg gaacctgcct gccgcaattt
     2281 tccgccgtgc gcgacgcctt cgccgccaat ttcgcggcgg acaggga
//
LOCUS       DASVLL010000093         5737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_274485,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000093 DASVLL010000000
VERSION     DASVLL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274485"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1825)
                     /locus_tag="VF402_01520"
     CDS             complement(128..1825)
                     /locus_tag="VF402_01520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7798996.1"
                     /translation="MSLNFKDQTFKDLGLSPTLLNTLEKAGYARPTPIQAQAIPILLA
                     GHDLLGIAQTGTGKTAAFALPILQHILTDRQIPEPKSVRVLVLSPTRELASQIADAFK
                     TYSAGLGLSIATIYGGMKYGPQYKALAKGLDILVATPGRLIDHIEQKTIDLRGVGFFV
                     LDEADQMLDMGFVKPIRQIAQRLPHKRQNLFFSATMPKEIGVLASELLTDPKKVEITP
                     EATTAEKVEQSVIFIEAQRKRALLSELYTDGALDRTLVFTRTKRGADRVAAYLQAGGV
                     EAAAIHGDKNQSQRERALQAFRAGKVRALVATDIAARGIDVDGVSHVINYELPMVAEA
                     YVHRIGRTARAGRSGVSITFCADDERRLLKDIERKTRQRIPFVDRRKDNALRLLDEAI
                     LASGNTEKPKTPDRLPEHKKPDQKRADARHSDPDAEFVHKRQRNRPGNKGRPGRAEKP
                     QRYDPMTAERAPSQAQDRKPHADRSRPDKNRADKARLVEKVRDMDPAGVFEPRAEGPR
                     KGRGGKPFGQKGGQKGGQNPQKFGERKSHKPNLVKGNAKSNAVKSGEGAQFKRRAKA"
     gene            2324..2782
                     /locus_tag="VF402_01525"
     CDS             2324..2782
                     /locus_tag="VF402_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7798997.1"
                     /translation="MTIPIRRPSPATPAPAAESKVEIIMPSQYADRRAEPRFNCDNRG
                     ALLLLPQGRAMPCRILDQSASGARISISDVNEIPAELWLIDLDTNTVRRGAAAWSMAN
                     RMGLKFNFIQKLTEGQARPAKVPEDVFAAWLKLSGQDESGPDDDGVLYFD"
     gene            2842..3411
                     /gene="moaB"
                     /locus_tag="VF402_01530"
     CDS             2842..3411
                     /gene="moaB"
                     /locus_tag="VF402_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX7798998.1"
                     /translation="MTAISDQDKQWLGGGRLDEDRPFRPVNIAVLTVSDSRDASNDTS
                     GDVLAARVADAGHRLIGRAIVRDDIDAIRARIRAWVASGEVDAIVSTGGTGITGRDVT
                     PEAVEPLLSKRIEGFSSIFHMLSYQTVGLSTLQSRALAGIIDGVFVFCLPGSNGAVKD
                     GWDKVIRWQLDSRHGPCNMVELMPRLKEK"
     gene            3430..4656
                     /gene="glp"
                     /locus_tag="VF402_01535"
     CDS             3430..4656
                     /gene="glp"
                     /locus_tag="VF402_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX7798999.1"
                     /translation="MSKLMPVADAAAAVRAAAPLMPAQKVRLHKAAGRVLAEAITATR
                     DQPPFAASAMDGYAVHTDSLHVETPVLRVIGESQAGRGFAGQVDVGDAVRIFTGAPVP
                     AGADTIVIQENVKREGQHIEVLPDGRNTAPRHIRPAGQDFKAGETLLQGGQRLDAWRL
                     ALVAAAGREAVSARRRPRVVVLCTGDELVAPGRTPGPDQIYESGSAALIALIRAWGGK
                     ATYAGFQADDLEATHKALKNVVKDHAPDLIVTIGGASVGDFDLVRPALNRLGLEAAFD
                     SVKVRPGKPTAFGRLGDGTQALMLPGNPASALVMAQLLLKTWIEAALGMEAGPKFVNA
                     VLQAPAPAAGPRETFLRGRLSSSLHGQLQVEAFSDQDSSLVGVFAQADALIRLPADAP
                     PMHAGGRVEILPLERL"
     gene            complement(4657..5655)
                     /locus_tag="VF402_01540"
     CDS             complement(4657..5655)
                     /locus_tag="VF402_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX7799000.1"
                     /translation="MPNTFVAFGELLIRLTATGGKLLESLPDLALHVGGAEANVCVGL
                     ARLGHATRMISVVPDNALGLAARDELRRWGVDTADVAQVPGRMGLYFLTPGAIHRPSD
                     IVYDRAGSAFAAADFAACDWGAALRGADWLHVSGVTAALGEASFNAALAAVKAANAAG
                     VKVSFDCNYRPKLWAAWGGDAPKLIPQLMAGADLIFGGHRDIELVSGQKFADARAAAD
                     HAFLSFPRLRRLVCTRREQVSADHNRLAGLMFTRDDTLETRMYEVAGIVDRIGGGDAF
                     AAGVLHGLIDGETDAKALDYGIACGCLKHAQPGDLSLATAADLDGFLSGDGFDVRR"
BASE COUNT          899 a         1909 c         1985 g          944 t
ORIGIN      
        1 cgcacacgca gtttgtgcgc ggctatttaa gggcaaaccg cctcatcaca caactgtgaa
       61 aatgccgcat cgccccggct ccccgtttgc acgaaagcgc gggagcgatg cggcgcatcg
      121 ccttcggtca ggccttggcc cggcgcttga attgcgcgcc ttcgccggac ttgacagcgt
      181 tcgatttggc gttgcccttg acgaggttcg gcttgtggct cttgcgctcg ccgaacttct
      241 gcgggttctg gccgcccttt tgaccgccct tttgaccgaa cggcttgccg ccacggccct
      301 tgcgcggacc ttcggcgcgc ggttcgaaca cgcccgccgg gtccatgtcg cgcacctttt
      361 cgacgaggcg cgccttgtcg gcgcggttct tgtcgggacg gctcctgtcg gcgtggggct
      421 tgcgatcctg cgcctgcgac ggcgcgcgct cggcggtcat cggatcgtag cgctgcggct
      481 tttcggcgcg gcccggacgg cccttgttgc cggggcggtt gcgctggcgc ttatgcacga
      541 attcggcgtc ggggtcgctg tggcgcgcat cggcacgctt ctggtccggc tttttgtgtt
      601 cgggcagacg gtccggcgtc ttcggctttt cggtgttgcc cgaggcgagg atggcctcgt
      661 ccagcaggcg cagcgcattg tccttgcggc ggtcgacgaa cgggatgcgc tggcgcgtct
      721 tgcgctcgat gtccttcagc aggcggcgct cgtcgtcggc gcagaaggtg atcgacacgc
      781 ccgaacgccc ggcgcgcgcg gtgcggccga tgcggtggac gtaagcctcg gccaccatcg
      841 gcagctcgta attgatgacg tgcgacacgc cgtcgacgtc gatgccgcgc gcggcgatgt
      901 cggtggcgac cagcgcgcgc accttgcccg cgcggaaggc ttgcagggcg cgctcgcgct
      961 ggctctggtt cttgtcgccg tggatggcgg cggcttcgac gcccccggct tgcaggtagg
     1021 cggccacgcg gtcggcgccg cgcttggtgc gggtgaagac cagggtgcgg tccagcgcgc
     1081 cgtcggtgta aagctcgctc aacagcgcgc gcttgcgctg cgcctcgatg aagatcacgc
     1141 tctgctcgac cttttcggcg gtcgtggctt cgggggtgat ctcgaccttc ttcggatcgg
     1201 tcagcaattc ggaggccagc acgccgatct ccttgggcat ggtggccgag aaaaacaggt
     1261 tctggcgctt gtgcggcagg cgctgggcga tctggcggat cggcttgacg aagcccatgt
     1321 ccagcatctg gtcggcctcg tccagcacga agaagccgac gccgcgcagg tcgatggtct
     1381 tttgctcgat atggtcgatc aggcggcccg gcgtggcgac cagaatgtcg aggcccttgg
     1441 ccagcgcctt atattgcggg ccgtacttca tgccgccgta gatggtggcg atggagaggc
     1501 cgagacctgc ggaataggtt ttgaacgcgt cggcgatctg cgaggccagt tcgcgcgtcg
     1561 gcgacaacac cagcacgcgc acgcttttcg gttcgggaat ctgacggtcg gtcaggatat
     1621 gttgcaggat cggcagggcg aaagccgccg tcttgccggt gccggtctgg gcgatgccca
     1681 gaaggtcgtg tccggccagc aggatcggaa tcgcctgcgc ctgaatcggc gtcgggcggg
     1741 catagcctgc cttttcgagg gtgttcaaca gggtgggcga caggcccaga tctttgaagg
     1801 tttggtcttt aaaattcagc gacacgcgcg aaactttcta atttaaaaac agcgcaaaac
     1861 gcgccgcgcc cgtctccggt ctgcggagcg ggcgatggat gggcgaagcg ttcgcgtggg
     1921 gcgcgtcgga tgatcaggcg gcggagcgtt cgatgacgat cctgaggggg gctggcaaat
     1981 acggacccgc gcatcggtgc ggacatatct cacgcggcca gccgggctca gtgcagaaaa
     2041 actctgggcg agccgttaag gtttcgcaac tgcgaagtcg cctaaatgcc cgcaaagccg
     2101 tgaaaagtca agcggcggta ttatcggcct gaaaaatgcg cctgtggacg gttccgcgcg
     2161 gcacaacgct ttttaaacga aaagggtcta ggctcgaccg cagagccgat ctcccgccgc
     2221 agacttcgtc aggcgggacg gccttcagta tcctggtttc cgcgtacagt gaaaaggctc
     2281 ctgcaaacag cagcgggccg tcgatttgca ggaccgatcc gacatgacca tcccgatccg
     2341 ccgaccatcg cccgcgacgc ccgctcccgc agcggaaagc aaggtcgaga tcatcatgcc
     2401 gtcgcaatac gccgatcgcc gcgccgagcc gcgcttcaac tgcgacaatc gcggcgcgct
     2461 gctgctgctg ccgcagggcc gcgccatgcc gtgccgcatc ctggaccaga gcgcgtccgg
     2521 cgcgcgtatc tccatctccg atgtgaacga aattcccgcc gagctgtggc tgatcgacct
     2581 cgacaccaac accgtgcggc gcggcgccgc cgcctggagc atggcgaacc gcatgggcct
     2641 gaagttcaac ttcatccaga agctgaccga gggtcaggcg cgcccggcca aggtgccgga
     2701 ggacgtgttc gccgcgtggc tgaagctcag cggccaggac gagagcggcc ccgacgacga
     2761 cggcgtgctc tatttcgact gactccgctt gagcggcccg cgcgcagggg ctatgcctga
     2821 cgcgacagtc aggagagcga gatgacggcg atcagcgacc aagataagca atggctcggc
     2881 ggcgggcgtc tcgatgagga ccgccctttc cggccggtca atatcgccgt gctgaccgtg
     2941 tccgacagcc gcgacgcaag caacgacacc tccggcgacg tgctggccgc gcgcgtcgcg
     3001 gacgccgggc accgcctgat cgggcgcgcc atcgtgcgcg acgacatcga cgccatccgc
     3061 gcgcgcatcc gggcgtgggt cgcctcgggc gaggtcgacg ccatcgtctc gaccggcggc
     3121 accggcatca ccgggcgcga cgtcacgccc gaggcggtcg agccgctgct gtccaagcgc
     3181 atcgagggct tttccagcat cttccacatg ctcagctatc agacggtcgg gctgtcgacc
     3241 ctgcaatcgc gcgcgctggc cgggatcatc gacggcgtgt tcgtcttctg cctgcccggc
     3301 tcgaacggcg cggtcaagga cggctgggac aaggtgatcc gctggcagct cgacagccgc
     3361 cacggcccct gcaacatggt cgaactgatg cccagactca aagagaaata atactcaaag
     3421 agaaataaca tgtccaagct catgcccgtc gccgacgccg ccgccgccgt gcgcgccgcc
     3481 gctccgctca tgccggcgca aaaggtccgg ctgcacaagg cggcggggcg cgttctggcc
     3541 gaggcgatca cggcgacgcg cgaccagccg ccgttcgcgg cctcggcgat ggacggttac
     3601 gccgtgcata ccgacagcct gcacgtcgag acgccggtgc tgcgcgtgat cggcgaaagc
     3661 caggccggac gcggcttcgc gggtcaggtc gatgtcggcg acgccgtgcg catcttcacc
     3721 ggcgcgccgg tcccggcggg cgccgacacc atcgtcattc aggaaaacgt caagcgcgag
     3781 ggccagcata tcgaggtgct gcccgacggc cgcaacaccg cgccgcgcca tatccgcccg
     3841 gcggggcagg atttcaaggc gggcgagacg ctgctgcaag gcgggcaaag gctcgatgcg
     3901 tggcggctgg ccctcgtcgc ggcggcgggc cgcgaggcgg tgagcgcgcg ccgccgtccg
     3961 cgcgtggtcg tgctttgcac cggcgacgaa ctggtcgcgc cgggacggac gccggggccg
     4021 gaccagattt acgagtccgg ctcagcggcg ctgatcgccc tgatccgggc gtggggcggc
     4081 aaggcgacct atgccggatt tcaggccgac gatctcgaag cgacccacaa ggcgctgaaa
     4141 aacgtggtga aagaccatgc gcccgacctg atcgtcacca tcggcggcgc cagcgtcggc
     4201 gatttcgatc tggtgcgtcc ggcgctgaac cggctcggtc tggaggccgc tttcgacagc
     4261 gtcaaggtcc gccccggcaa gccgacggcg ttcggccggc tgggcgacgg gacgcaggcg
     4321 ctgatgctgc ccggcaatcc ggcctcggcg ctggtgatgg cgcaactgct gctcaagacc
     4381 tggatcgagg cggcgctcgg catggaggcg gggccgaaat tcgtcaacgc cgtgctgcag
     4441 gccccggcgc ccgccgccgg tccgcgcgaa acgtttttgc gcggccggct gagttcgtcc
     4501 ttacacggac aattgcaggt cgaggctttt tccgatcagg attcctcgct ggtcggcgtg
     4561 ttcgcccagg ccgacgcgct gatccgcctg cccgccgacg ccccgccgat gcatgcgggc
     4621 gggcgcgtgg agatcctgcc gctggagcgg ttgtgatcag cgccggacgt cgaagccgtc
     4681 gccgctgagg aagccgtcca gatccgccgc cgtcgccagc gacagatcgc ccggctgcgc
     4741 gtgcttcagg cagccgcagg cgatgccgta atccagcgcc ttcgcgtccg tttcgccgtc
     4801 gatcagcccg tgcagcacgc ccgccgcgaa ggcgtcgccg ccgccgatgc ggtcgacgat
     4861 gcccgccacc tcgtacatcc gcgtttccag cgtatcgtcg cgcgtgaaca tcagcccggc
     4921 caggcggtta tggtcggcgc tgacctgttc gcggcgcgtg cagacgaggc ggcgcaggcg
     4981 cgggaagctc aggaaggcgt gatcggcggc ggcgcgcgcg tccgcgaatt tttgccccga
     5041 caccagctcg atgtcgcggt gaccgccgaa gatcaggtcg gcgcccgcca tcagttgcgg
     5101 gatcagcttg ggcgcgtcgc cgccccacgc cgcccacagc ttcgggcggt aattgcagtc
     5161 gaacgacacc ttcacgcccg ccgcattggc cgccttgacc gccgccagcg cggcgttgaa
     5221 gctggcctcg cccagcgccg ccgtcacgcc cgacacatgc agccagtccg cgcccctgag
     5281 cgccgcgccc cagtcgcagg cggcgaaatc cgcggcggcg aaggcggagc cggcgcggtc
     5341 gtaaacgatg tcggacgggc gatgaatggc gcccggcgtc aggaaataga ggcccatgcg
     5401 gcccggaacc tgcgccacgt ccgccgtgtc gacgccccag cggcgcagct cgtcgcgcgc
     5461 ggccagcccc agagcgttgt cgggaaccac gctgatcatg cgcgtcgcat ggcccagccg
     5521 cgccagcccg acgcagacat tggcctcggc gccgccgaca tgcagggcca ggtcgggcag
     5581 gctttccagc agcttgccac cggtggcggt gaggcggatc agcagttcgc cgaaggcgac
     5641 gaaggtgttg ggcatggcgt gatttttcct gttttgcggc gttttaaagc gccggaaccg
     5701 gaaagggaag ccccgcccgc gacaatcccg gccctag
//
LOCUS       DASVLL010000094         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_5355, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000094 DASVLL010000000
VERSION     DASVLL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5355"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VF402_01545"
     CDS             complement(<1..351)
                     /locus_tag="VF402_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HEX7799001.1"
                     /translation="MMSWLNPDKTHPISLPDGTEIRNTVYLNKAIDHPRLTAGDFSYA
                     SSFDPVTDWAQTLAPYLFPISREKLVIGRFCQIAHGARFITSSANHPMGGFSTFPFRV
                     LRPETMGDYLNLPFR"
     gene            complement(348..869)
                     /locus_tag="VF402_01550"
     CDS             complement(348..869)
                     /locus_tag="VF402_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799002.1"
                     /translation="MSAPSLDHHRRAAVRLLKALRGDDALRHASAVDRFARLALRIPP
                     QTFQLKHALAVVADEAGYPNWAALKQACEGPDFSEVFAAPGLKDSLNAWFTAYEDAAA
                     HRAETDGVLLPYRHQMFVTSRAVLPRLGFEADDPDWRDIGYDFVRPASDAAHARIAAA
                     LTRRFLLTHEKVR"
     gene            1147..>1915
                     /locus_tag="VF402_01555"
     CDS             1147..>1915
                     /locus_tag="VF402_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7799003.1"
                     /translation="MRPVSEQRIEPGPADFAPEAPPLLVRKPLPYADRWWRSEDGLQL
                     YARDYPAVAGRARLPVICLHGMRRNSADFEAVAPYIAALERRVIAPDLRGRGLSENDP
                     DAGNYHLWTYARDVAQMCDAFGIGQAVVIGSSMGGLAAMVLATLRPNLVRATVLNDVG
                     PKPARKGLARIAARAGADPAPHDDWAGLAAALAEQSGEDYPNYKAADWLNMARRLYRK
                     GRDGRLHPAFDARLTAACRHLTLDLAAYDLEPCYASLA"
BASE COUNT          313 a          616 c          682 g          304 t
ORIGIN      
        1 gcggaacggc aggttcagat agtcgcccat cgtctccggg cgcagcaccc ggaacggaaa
       61 ggtcgagaac ccgcccatcg gatgattggc cgacgaggtg atgaagcgcg cgccgtgggc
      121 gatctggcag aagcgcccga ttaccagctt ttcgcggctg atcgggaaca gatagggcgc
      181 cagggtctgc gcccagtccg tcaccggatc gaagctggac gcataggaaa agtcgcccgc
      241 cgtgaggcgc ggatggtcga tcgccttgtt gagatagacg gtgttgcgga tctcggtccc
      301 gtcgggcagc gagatcggat gggtcttgtc gggattgagc cagctcatca tcggaccttt
      361 tcatgtgtca acagaaagcg ccgcgtcagg gcggcggcga tgcgcgcatg cgccgcgtcc
      421 gaggcgggcc gcacgaaatc gtagccgatg tcgcgccagt cgggatcgtc ggcttcaaag
      481 ccgagccggg gcaggacggc ccttgacgtc acgaacatct gatggcggta gggcagcagc
      541 acgccgtccg tttccgcgcg gtgcgcggcg gcgtcctcgt acgccgtgaa ccacgcattg
      601 agcgaatcct tcaggccggg cgcggcgaag acttcggaaa aatccggccc ttcgcacgcc
      661 tgtttcaacg ccgcccagtt ggggtaaccg gcctcgtcgg cgacgacggc cagggcgtgc
      721 ttgagctgaa aggtctgcgg cgggatgcgc agggccaggc gcgcgaaccg gtccacggcc
      781 gaggcgtggc gcagggcgtc gtcgccgcgc agggccttga gcaggcgcac ggcggcgcgg
      841 cggtgatggt cgagagaagg ggcggacatg acgtctcctt tgcgaaacgg cccggtcccc
      901 atcccggtcg ggccaacaaa gaagtcttta agattgtcgt cggcgaaatc ttgcgtccgg
      961 acggcttgcg ccttgcgtgg ggcggggcct ttccggcagg cccgtcccgg cgatagcgcg
     1021 acggggcgaa aaattcaagc gccaaacgcg cctcgttcac acgtccgatg cacacgtttg
     1081 gggcgttagg cttatcttta ccccctgccc taatgatgcg taaaaactgg aaatatgagc
     1141 ggaaccttgc gccccgtcag cgaacagcgg attgaacccg gccctgcgga cttcgccccc
     1201 gaagcgccgc cgcttctggt ccgcaagccg ctgccgtacg cggaccgctg gtggcgcagc
     1261 gaggacgggc tgcaactcta tgcgcgcgac tatccggcgg tggccgggcg ggcgcgcctg
     1321 ccggtgatct gcctgcacgg catgcgccgc aacagcgccg atttcgaggc ggtcgccccc
     1381 tatatcgccg cgcttgagcg ccgggtcatc gcgccggacc tgcgcgggcg cggcctgtcc
     1441 gagaacgatc ccgacgcggg caattaccat ctgtggacct atgcgcgcga cgtggcgcag
     1501 atgtgcgacg ccttcggcat cggtcaggcc gtggtgatcg gcagctcgat gggcgggctg
     1561 gcggcgatgg tgctggccac cttgcgcccg aatctggtgc gcgccaccgt gctgaacgat
     1621 gtcgggccga agccggcgcg caaggggctg gcgcgcatcg ccgcgcgcgc aggcgccgat
     1681 ccggcgccgc atgacgactg ggccgggctg gccgccgcgc tggccgaaca atcgggcgag
     1741 gactatccga actataaggc ggccgactgg ctgaacatgg cgcggcgtct ttatcgcaag
     1801 gggcgcgacg gccggttgca tccggccttc gacgcgcgcc tgaccgccgc ctgccgccat
     1861 ctgacgctcg atctggccgc ctacgatctg gagccgtgct acgccagtct ggcgc
//
LOCUS       DASVLL010000095         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_435210,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000095 DASVLL010000000
VERSION     DASVLL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435210"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VF402_01560"
     CDS             complement(<1..1040)
                     /locus_tag="VF402_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX7799004.1"
                     /translation="MTHFDSFQHYQDQRQRLIEGHAYDPATEKDACGVGVVCAIDGSP
                     RRDVVELAVKALKALFHRGAVDADGKSGDGAGIMINVPQAFFRKQIEQIGHKPRPGPI
                     LVGQVFLPRTDLGAQETARTIVESEILRSGFYLYGWRQPPVDVAQLGERASATRPEIE
                     QIMMAAPEGLEGEALERAMFLCRRRIEKRVDEANLGDFYLCSFSAKTLIYKGMFRAEL
                     VDDFYLDLKDPEFVSSVAIFHQRFSTNTFPEWRLAQPFRMLAHNGEINTLRGNINWMK
                     SHEIRMAAATFGDQAKDVKPVIQPRGSDSAALDNVFELLVRAGRSAPMAKALLIPEAV
                     AKDANVSAANKAL"
     gene            complement(1043..2515)
                     /locus_tag="VF402_01565"
     CDS             complement(1043..2515)
                     /locus_tag="VF402_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7799005.1"
                     /translation="MLMDTNRFVVEVKKTPKKSDAKTRVANFNEIYADFTPNEASHQA
                     SRCAQCGVPFCQHGCALQNNIPDWLRLTAEGRVEEAYRLSSATSALPEICGRICPQDR
                     LCEGSCVLEQSGWENVTIGAVERYINDKAFEEGWIEAIAPAVERPQSIGIIGSGPAGI
                     AAADRLRTLGYKVTVYDRHDRAGGLLTYGIPSFKLEKEVVARRTDRLAASGVDFVLNV
                     NIGEDVAFEELREKHDAILIATGVYKARRLSVPGCGSRGVAAALDFLITANKIGFGDT
                     IADFDNGRLNAKGKNVVVIGGGDTAMDCVRTSIRQGARSVTCVYRRDRANMPGSDREV
                     ANAEDEGVRFEWLSAPKAVLGDADKVSGLKIQRMRLTTPDAQGRQAVEEIHGAETDLP
                     ADLIIEALGFEPENLPVLFNQPGLEVTRWGTIRTLAGEFQSMVPGVFAAGDIVRGASL
                     VVWAVREGQDAAADIHHYLQTEALSAADADVEALALGAAE"
     gene            complement(2826..4103)
                     /locus_tag="VF402_01570"
     CDS             complement(2826..4103)
                     /locus_tag="VF402_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7799006.1"
                     /translation="MAILSTVLSTPAPLLAHQAALALNSGDTAWMLVSTALVLFMTLP
                     GVALFYGGMVRKKNLLATMAQSVAVTVIASVIWALAGYSLAFGVNASAGVNAVIGSFE
                     ALGLHGVRPDTAFAGAPQLPEYLWIAYQMTFAIITPALIAGSIVERMKFSAFCLFIAL
                     WSVFVYAPVCHWVWGGGFLGKMGVLDFAGGAVVHVNSGVAGLVACLVIGKRKGFGRDN
                     MAPHNLGYTLIGASLLLVGWLGFNAGSELKADGLAAAAALNTILAAMTGAIGWTVPEW
                     IERKQPTLLGMVSGVIAGLVAITPAAGFVGPDGALLIGLITGPICYLSSTYVKNFFRY
                     DDSLDAFGVHAVGGFTGALLTAVFADPAINTAGANANVLKQLIGLIAVIAWSAAVSFL
                     ILMVCKATTGLRVTEEQEIEGLDQSQHGETLHG"
BASE COUNT          770 a         1432 c         1402 g          718 t
ORIGIN      
        1 agcgccttgt tcgccgccga gacgttggcg tccttggcga ccgcttcggg gatcagcagc
       61 gccttggcca tcggggccga gcggcccgcg cgcaccagca gctcgaacac gttgtcgagc
      121 gcggcggagt ccgagccgcg cggctggatc accggcttca cgtccttggc ctgatcgccg
      181 aaggtggcgg cggccatgcg gatctcgtgc gacttcatcc agttgatgtt gccgcgcagc
      241 gtgttgatct cgccgttgtg cgccagcatg cggaacggct gcgccagccg ccattccggg
      301 aaggtgttgg tcgagaagcg ctggtggaag atcgccaccg acgagacgaa ttccgggtct
      361 tttaagtcga gatagaagtc gtcgaccagc tcggcgcgga acatgccctt gtagatcagc
      421 gtcttggccg agaaggaaca caggtagaag tcgccgagat tggcctcgtc gacgcgcttt
      481 tcgatgcggc ggcggcacag gaacatggcg cgctccagcg cctcgccctc aaggccctcc
      541 ggcgccgcca tcatgatctg ctcgatttcc gggcgcgtgg ccgaggcgcg ctcgcccagt
      601 tgcgccacgt ccaccggcgg ctggcgccag ccgtagaggt agaagcccga acgcaggatt
      661 tccgactcca cgatggtgcg cgccgtctcc tgcgcgccga ggtcggtgcg cggcaaaaag
      721 acctggccga ccaggatcgg gccggggcgc ggcttgtggc cgatctgctc gatctgcttg
      781 cggaagaagg cttgcggcac gttgatcatg atgcccgcgc cgtcgcccga cttgccgtcg
      841 gcgtcgaccg ccccgcggtg gaacagggcc ttgagcgcct tgacggccag ctcgacgacg
      901 tcgcggcgcg gcgatccgtc gatggcgcag acgacgccga cgccgcaggc gtccttttcg
      961 gtggccggat cgtaggcgtg gccttcgatc aggcgctgac gctggtcttg gtaatgctga
     1021 aacgagtcaa aatgggtcat gattattccg cggctcccag agccagagcc tccacatcgg
     1081 cgtcggccgc cgagagcgct tcggtttgca gatagtggtg aatgtcggcg gcggcgtcct
     1141 ggccctcgcg cacggcccag acgaccagcg aggcgccgcg cacgatgtcg cccgcggcga
     1201 acacgcccgg caccatcgac tggaactcgc ccgccagcgt gcggatcgtg ccccagcgcg
     1261 tgacctccag ccccggctgg ttgaacagca ccggcaggtt ctccggctcg aagcccagcg
     1321 cctcgatgat caggtcggcg ggcaggtcgg tttcggcgcc gtggatctcc tcgacggctt
     1381 gccttccttg cgcgtccggc gtggtcaggc gcatgcgctg gatcttcagg ccgctcacct
     1441 tgtcggcgtc gcccagcacc gccttgggcg cggacagcca ttcgaagcgc acgccctcgt
     1501 cctcggcgtt ggcgacctcg cggtccgagc ccggcatgtt ggcgcggtcg cggcgataga
     1561 cgcaggtgac cgaccgggcg ccctgacgga tcgaggtgcg cacgcagtcc atcgccgtgt
     1621 cgccgccgcc gatgacgacg acgttcttgc ccttggcgtt gaggcggccg ttgtcgaagt
     1681 cggcgatcgt gtcgccgaag ccgatcttgt tggccgtgat caggaagtcg agcgcggcgg
     1741 ccacgcccct tgagccgcag cccggaacgc tgaggcggcg cgccttatag acgccggtgg
     1801 cgatcaggat ggcgtcgtgc ttttcgcgca gctcctcgaa ggcgacgtcc tcgccgatat
     1861 tgacgttcag cacgaagtcg acgcccgacg ccgccagacg gtcggtgcgg cgcgccacga
     1921 cctccttctc cagcttgaac gacgggatgc cgtaggtcag aagcccgccc gcgcggtcgt
     1981 ggcggtcata gaccgtgacc ttgtaaccga gcgtgcgcag gcggtcggcg gcggcgatgc
     2041 ccgccggacc ggagccgatg atcccgatgg actgcggccg ctcgacggcg ggcgcgatcg
     2101 cctcgatcca gccttcctcg aacgccttgt cgttgatgta gcgctcgacc gcgccgatgg
     2161 tcacgttctc ccagccggac tgctccagca cgcacgagcc ttcgcacaga cgatcctgcg
     2221 ggcagatgcg gccgcagatt tccggcaggg ccgaggtcgc cgaggacagg cggtacgcct
     2281 cctcgacgcg cccttcggcg gtcaggcgca gccagtcggg aatgttgttc tgcaacgcgc
     2341 agccatgctg gcagaacggc acgccgcact gggcgcaccg cgaggcctga tgcgaagcct
     2401 cgttcggtgt gaagtcggcg tagatctcgt tgaagttggc cacgcgcgtc ttcgcgtccg
     2461 acttcttcgg ggtctttttg acttcaacca caaaacggtt cgtatccata agcatagcct
     2521 ttacatgccg ggtaagcccg gctgcgaccg gaacgtccgg ccccgcgtct gcgctgaacc
     2581 ttgctaaaag cgcaaaagtc ccgcgaagaa aagtcttaat cccagccctg cggcatattt
     2641 gtcatgtttt cgcccgttat gacaaaattg ccaaacccgt atcgaaagat acgataaggg
     2701 cctgaagtca acatgcaaac cccctcggct gatcctgaag acggctttaa caggtcttgc
     2761 ctgcctgccg ggcctcaggt caatcgcgag gggggagatt tggtcagccg ctgcaatcta
     2821 gcagattatc cgtgcagcgt ctccccgtgt tgcgattgat cgaggccctc gatctcctgc
     2881 tcctccgtga cgcgaaggcc tgttgtcgcc ttgcatacca tgaggatcag gaagctgacc
     2941 gccgcgctcc acgcgatgac ggcgatcagg ccgatcaact gtttgagcac gttggcgttc
     3001 gcgcccgccg tgttgatcgc cggatcggcg aacaccgctg tcagaagcgc gcctgtaaac
     3061 ccgccgaccg catgcacgcc gaacgcgtcg agcgagtcgt cgtaacgaaa gaagttcttc
     3121 acataggtcg aagacagata gcagatgggg ccggtgatca ggccgatcaa gagcgcgccg
     3181 tccggcccga cgaaaccggc ggcgggcgtg atggcgacaa ggcccgcgat cacgccggac
     3241 accatgccca gaagggtcgg ctgcttgcgc tcgatccact ccggcacggt ccagccgatg
     3301 gcgccggtca tggcggccag gatggtgttg agcgcggcgg cggcggccag cccgtcggcc
     3361 ttcagctccg aaccggcgtt gaagcccagc cagccgacca gcagcagcga cgcgccgatc
     3421 agcgtatagc cgagattatg cggcgccatg ttgtcgcgcc cgaagccctt gcgcttgccg
     3481 atcacgaggc aggcgaccag gcccgcgacg ccggaattga catgcacgac cgcgccgccg
     3541 gcgaaatcca gtacgcccat cttgccgagg aagccgccgc cccagaccca gtggcagacc
     3601 ggcgcataga cgaacacgct ccacagcgcg atgaacaggc agaaggccga gaacttcatg
     3661 cgctcgacga tcgagcccgc gatcagggcc ggggtgatga tcgcgaaggt catctgatag
     3721 gcgatccaca gatattccgg cagttgcggc gcgcccgcga aggcggtgtc ggggcgcacg
     3781 ccgtgcaggc ccagcgcctc gaacgagccg atgaccgcgt tgacgcccgc cgacgcgttg
     3841 accccgaagg ccagcgaata accggccagc gcccagatca ccgaggcgat gacggtgacc
     3901 gccaccgact gcgccatcgt cgccagcagg ttcttcttgc gcaccatgcc gccgtagaac
     3961 agcgccacgc ccggcagggt catgaacagc accagcgccg tcgaaaccag catccacgcc
     4021 gtgtcgcccg aattcagggc aagcgccgcc tgatgcgcca gaagcggcgc aggcgtgctc
     4081 aaaacggtcg acaggatggc catcaggcgt cagctctcca aaagaaaatc aggtcacagc
     4141 gcgacaggcc gcgaagctct ggcgcttcgt gtgaaaccgc gaattgtgac ggatcgcggc
     4201 ctcatagcag gatggaacgt gttttaaaag tcaaagcttc cgacaaatat gcccattctt
     4261 cggaaatttt ccgaacccgc cttcacgatg ccgtcacaat cagcaaagcg acgttatacc
     4321 cc
//
LOCUS       DASVLL010000096         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_58366, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000096 DASVLL010000000
VERSION     DASVLL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58366"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VF402_01575"
     CDS             complement(<1..179)
                     /locus_tag="VF402_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX7799007.1"
                     /translation="MPINRLAKQVVDELRGRRMTIATVESCTGGLLSAAITGVAGASD
                     VFGYGFVTYANAAKT"
     gene            complement(233..847)
                     /locus_tag="VF402_01580"
     CDS             complement(233..847)
                     /locus_tag="VF402_01580"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7799008.1"
                     /translation="MTFELPPLPYAADALEPYMSANTFSFHHAKHHKAYVDNLNKALP
                     GSEFEGKSLVEIVKASEGKNPGVFNNAAQVWNHTFFWHSMKPNGGGAATGKIADRINA
                     DFGSFDAFVEQFKTAGATQFGSGWAWLVLGSDGKLKVVKTPNGENPFTKGDKPLLTLD
                     VWEHAYYLDYQNLRPKFIETFLDKLVNWDFANERLEAPLFEGVK"
     gene            complement(1017..2156)
                     /locus_tag="VF402_01585"
     CDS             complement(1017..2156)
                     /locus_tag="VF402_01585"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX7799009.1"
                     /translation="MTFHAIIVAGGSGSRSGGRKQWMPLGGRAVLDWSVDAFAAAGAA
                     QIVVVVPLDDVARAEAAYGDRAQVTAGGAQRAESVVNGLRALTAEADDIVMIHDAARP
                     LLSASHIGGLLTALATQRAAILALPVTDTLKKGAGGVIADTPPRDDLWRAQTPQAFRY
                     GDIVSAYAAWPQDRNATDEAMVAAHAGIDVALVAGDMRLHKLTYPEDFALLEAFIREE
                     TRVRDIRTGQGFDAHRWGDGDSVWLCGVEIPHDQTLIGHSDADAGLHALTDALLGAAG
                     LGDIGDHFPPGDPQWKGAASDRFLRHAADLIAGRGGRVLNVDVTLICERPKIKPVREA
                     MRRRVAEILGLDVERVSVKATTTEGMGFAGRGEGLAASAVCTIEF"
     gene            2278..>3136
                     /locus_tag="VF402_01590"
     CDS             2278..>3136
                     /locus_tag="VF402_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX7799010.1"
                     /translation="MSHKTLQIGDVAVPGRVLIAPMTGVSDLPFRRMAHRLGAPYAAT
                     EMVACAEMETGRADVARKAQLGEHDGLKIIQLVGRDPAHIARGARLAEDMGAHIVDLN
                     FGCPAKEVTGVLCGSALMRDLDQAERIMTAALGATSRPVTVKMRLGWDDDSRNCAELA
                     LRAQSLGVKALTVHGRTRQQFYKGEADWRAVAEVRQAVRIPVIVNGDVVDLAGARAAL
                     AQSGADGVMIGRGIYGRPWFAHALDRALNDNEPLREPDAARRADILIEHMRDSLDFYG
                     AALRLRMFKK"
BASE COUNT          507 a         1027 c         1078 g          524 t
ORIGIN      
        1 tcggtcttgg cggcgttggc gtaggtgacg aagccatagc cgaacacgtc ggacgcgccc
       61 gccacgccgg tgatcgcggc ggacaaaagc ccgcccgtgc agctttccac ggtcgcgatc
      121 gtcatccgcc tgccgcgcag ttcatcgacg acctgtttcg ccagcctgtt gatgggcatg
      181 tgtgcgtccc ggcgcaataa aggcgcggat cgaaacccgc gccccgcttt tgttacttca
      241 cgccttcaaa caagggcgct tcgaggcgct cattggcgaa gtcccagttg accagcttgt
      301 cgaggaaggt ctcgatgaac ttcggacgca ggttctgata gtcgagatag taggcgtgct
      361 cccacacgtc gagggtcagc agcggcttgt cgcccttggt gaacgggttt tcgccgttcg
      421 gcgtcttcac gaccttgagc ttgccgtccg aaccgaggac cagccacgcc cagcccgaac
      481 cgaactgggt ggcgcccgcc gtcttgaact gttcgacgaa ggcgtcgaag gagccgaaat
      541 cggcgttgat cctgtcggcg atcttgccgg tggccgcgcc gccgccgttc ggcttcatcg
      601 aatgccagaa gaaggtgtgg ttccacacct gggcggcgtt gttgaacacg ccgggattct
      661 tgccttccga cgccttgacg atctcgacca gcgacttgcc ctcgaattcg ctgcccggca
      721 gcgccttgtt caggttgtcg acataggcct tgtggtgctt ggcgtggtgg aagctgaagg
      781 tgttggccga catgtacggc tcaagcgcgt cggcggcgta aggcaggggg ggcagttcaa
      841 atgtcatggc ggacctctca gcgtaaatat agggggaaga tacgaacctt caggtactat
      901 cggccttgct gatatattgg cctgaggacg gtattgcaag gcgggaacgc gcaaattagg
      961 gcgcaggatc accataaaaa ttcaatggtt tttcagcgct ttattgagaa ccgttatcaa
     1021 aactcgatcg tgcagaccgc gctggccgcc agcccttcgc cgcgtccggc gaagcccatg
     1081 ccttcggtgg tcgtggcctt gaccgacacc cgctcgacgt cgaggcccag aatctccgcg
     1141 acgcggcggc gcatggcctc gcgcaccggc ttgatcttcg ggcgctcgca gatcagcgtg
     1201 acgtcgacat tgaggacgcg cccgccgcgt ccggcgatca ggtcggcggc gtggcgcagg
     1261 aaacggtcgg aggcggcgcc cttccattgc ggatcgccgg gcggaaaatg atcgccgata
     1321 tcgcccagcc ccgccgcgcc cagcagcgcg tcggtcaggg cgtgcagccc ggcgtcggcg
     1381 tcggaatggc cgatcagggt ctggtcgtgc ggaatttcga cgccgcacag ccacacgctg
     1441 tcgccgtcgc cccagcggtg ggcgtcgaac ccttgcccgg tgcggatgtc tctcacgcgc
     1501 gtctcctcgc ggataaaggc ttcgagcagg gcgaagtcct cgggataggt cagcttgtgc
     1561 aggcgcatgt cgcccgcgac cagcgcgacg tcgatcccgg catgggcggc gaccatcgct
     1621 tcgtcggtgg cgttgcggtc ctggggccac gcggcgtagg ccgaaacgat gtcgccgtaa
     1681 cggaaggcct gcggcgtctg cgcgcgccac aggtcgtcgc gcggcggggt gtcggcgatg
     1741 acgccgcccg cgcctttctt cagcgtgtcg gtcacgggca gggccaggat ggcggcgcgc
     1801 tgagtcgcca gcgcagtcag caggccgccg atatggcttg cgctcagcaa gggacgcgcg
     1861 gcgtcgtgga tcatcacgat gtcgtccgcc tccgccgtca gcgccctcag gccgttgacg
     1921 accgactccg cccgctgcgc gccgcccgcc gtcacctgcg cgcgatcgcc gtaagccgcc
     1981 tccgcgcgcg ccacatcgtc cagcggaacg accacgacga tctgcgccgc gcccgccgcc
     2041 gcgaacgcgt ccacggacca gtccagcacg gcgcggccgc ccagcggcat ccactgcttc
     2101 ctgccgcccg agcggctgcc ggacccgccg gcgacgataa tggcgtgaaa agtcatggac
     2161 gctgtttagc gcgaaaccgg gaggctgcaa cgaagaaatg cggcggggcg ggtttttgcc
     2221 cgggacggcg tcaaccttcg ttggccatcc gcatggaaat cctgtatgag cgcggcgatg
     2281 agccataaga ccttgcagat cggagacgtc gccgtaccgg gccgggtgct gatcgcgccg
     2341 atgacgggcg tgtcggacct gccgttccgg cgcatggcgc accggctcgg cgcgccctat
     2401 gcggcgaccg agatggtggc gtgcgccgag atggagacgg gccgcgccga tgtggcgcgc
     2461 aaggcgcaac tgggcgagca tgacggcctg aagatcatcc agcttgtcgg gcgcgacccg
     2521 gcgcacatcg cgcgcggcgc gcggctggcc gaggacatgg gcgcgcatat cgtcgacctc
     2581 aacttcggct gtccggccaa ggaggtcacc ggcgtgctgt gcggctcggc cctgatgcgc
     2641 gacctcgatc aggccgagcg catcatgacg gcggcgctgg gcgccacgtc gcggccggtc
     2701 acggtcaaga tgcgcctcgg ctgggacgac gacagccgca actgcgccga gttggcgctg
     2761 cgggcgcaaa gcctcggcgt caaggcgctg accgtgcatg gccgcacgcg ccagcaattt
     2821 tacaagggcg aagccgactg gcgcgccgtg gccgaggtca ggcaggcggt gcgtatcccc
     2881 gtcatcgtca acggcgacgt cgtcgatctg gccggcgccc gcgcggctct ggcgcaatcg
     2941 ggcgcggacg gcgtgatgat cgggcgcggg atctacggcc gcccgtggtt cgcgcacgcc
     3001 ctggaccggg cgctcaacga caatgaacct ttgcgcgaac cggacgcggc gcgccgcgcg
     3061 gacattctga tcgagcatat gcgcgacagc ctcgacttct acggcgcggc gctgcgcctg
     3121 cgcatgttca agaagc
//
LOCUS       DASVLL010000097         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_435399,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000097 DASVLL010000000
VERSION     DASVLL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435399"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF402_01595"
     CDS             complement(<1..559)
                     /locus_tag="VF402_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7799011.1"
                     /translation="MSFDKAEPAPRRPKLKLPYDQTVLVLQGGGALGAYQAGAYEVLH
                     EGGVEPDWIAGISIGAINAAIIAGNAPEDRVPALRAFWQEIATTIPGEILAGQHAHSE
                     FNFRQFSGWLSAAAGVKGFFRPWFLPPWFNAPGTPEATSFYDIAPLRETLSRHVDFDR
                     INKGPLRLSLGAVQVRTGNFVYFDTR"
     gene            complement(626..1492)
                     /locus_tag="VF402_01600"
     CDS             complement(626..1492)
                     /locus_tag="VF402_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7799012.1"
                     /translation="MRRLILIAGLFLAATPALAQTAAPAPSPYDKAPWWMKESVITQT
                     GYVFTELPANRANFSASFQTTDDTADKAQSKAVAQTKALMQALDKLGKDAVRVTTDFS
                     MRTLYEQYRDKNGEKVENQRGDKINGYQVSLNLNVEVRDMTQLERAYALVLAASPDST
                     GQVYFSLQADNATNTWLYTEAVQDAHVRALQAAGAAGAKLGAVKVIDPTGRACETDIL
                     AGKQSYEGDGLRAQNVTVAEMRKVAAPAPSPPSAPGSAEALEAKALQNPFIQTPPMRR
                     LDAKACVVYALD"
     gene            1873..>2932
                     /locus_tag="VF402_01605"
     CDS             1873..>2932
                     /locus_tag="VF402_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7799013.1"
                     /translation="MRADDKGERRSDSRPHALGDSLSGTNLERAGDHNQRVTLQAVRA
                     AGAPITRAEIAELTGLTAPAIANITKRLLGDGMILKAGRLTGGRGQPATKLVVNPDGA
                     FSIGLNIDRDHIAIVALDFLGNVRFRTCREVHFAMPQEVIAFFREQVDMIIAQKAINI
                     ERLIGIGIGIPDDLGRVPVANRPDAYRIWSEIDVAALFAEILPTPVPTPVYVENDAAA
                     ASIGEMQFGHGLQSRSFIYTIISAGLGGGLVIDGHYYRGMNGQSGEIGFLPVRTDSGE
                     EKSLEDLVSLYALYDDLRKGGFDVSTPDDLEQLSEAGQARVEAWLDRAAEHLTQPFLV
                     MNCAIDPEAHYIGGRLPAA"
BASE COUNT          496 a          987 c          934 g          515 t
ORIGIN      
        1 cgcgcgtgtc gaaatagacg aaattgccgg tgcgcacctg cacggcgccg aggctcaggc
       61 gcaaaggccc cttgttgatg cggtcgaaat cgacatggcg cgacagcgtc tcgcgcaagg
      121 gggcgatgtc gtagaagctg gtcgcttccg gcgttccggg cgcattgaac cacggcggca
      181 ggaaccacgg gcggaaaaag cccttcaccc ccgccgccgc gctcagccag ccgctgaact
      241 ggcggaagtt gaactcgctg tgcgcatgct gaccggccag gatttcgccc ggaatggtcg
      301 tggcgatctc ctgccagaag gcgcgcaagg cgggcacgcg gtcttcgggc gcgttgcccg
      361 cgatgatggc ggcgttgatc gcgccgatgg agatgcccgc gatccagtcc ggctcgacgc
      421 cgccctcgtg cagcacctca taggccccgg cctgataggc gcccagcgcg ccgccgcctt
      481 gcaggaccag cacggtctgg tcgtaaggca gtttcagctt cgggcgccgg ggcgcgggtt
      541 cagccttatc gaaagacatg atgcgtctcc gtccatgcga ccggtctcat attgcatcgc
      601 aatatggccg tttcaagccc ggacatcagt cgagcgcgta caccacgcag gctttcgcat
      661 cgaggcgccg catcggcggc gtctggatga acgggttttg cagggccttg gcctcaagcg
      721 cttcggcgga accgggggcc gagggcggag acggcgcggg cgccgcgact ttacgcatct
      781 ccgccaccgt gacgttctgc gcccgtaagc catcgccttc ataactttgc ttacccgcca
      841 gaatgtccgt ctcgcacgcc cgtcccgtcg ggtcgatcac cttgacggcg cccagtttcg
      901 cgcccgccgc cccggccgcc tgaagcgcgc ggacatgcgc gtcctgaacc gcctcggtat
      961 agagccatgt gttcgtggcg ttgtcggcct gaagcgagaa atagacctgt ccggtcgaat
     1021 ccggcgacgc cgccagcacc agcgcatagg cgcgttcaag ctgcgtcatg tcgcgcacct
     1081 cgacattgag gttcaaactg acctgatagc cgttgatctt gtcgccgcgc tggttttcga
     1141 ccttctcgcc gttcttgtcg cgatactgct cgtagagggt gcgcatggaa aaatcggtgg
     1201 tgacgcgcac cgcgtccttg cccagcttgt ccagcgcctg catcagggct ttcgtctgcg
     1261 cgacggcttt cgactgcgcc ttgtccgccg tgtcgtcggt ggtctggaaa ctggccgaaa
     1321 aattcgcgcg gttggcgggc agttcggtga agacgtagcc ggtctgggtg atcacgctct
     1381 ccttcatcca ccacggcgcc ttgtcgtaag gcgacggcgc gggcgcggcg gtctgggcca
     1441 gcgcgggcgt cgcggccaga aaaaggccag cgatcagaat aaggcgtcgc atgatatccc
     1501 ccctgtcggt tgcatcgcgc aggctaacac gcgcccgcgc gggccgccag accttccgcg
     1561 cttcacaaac acatggcgat ctgctaattg taaccgatta caaatatcgc gcccgaccgc
     1621 cgccttccgg ccttcgccgg tctccggcgc gcgcgcgaaa tccagccgga cgatttcagg
     1681 gctgaattaa ttaaatctgt tttacaattc gcgacgacag cgcctataag gttccgttgc
     1741 gcggaaggaa gtcttgcgtc cggcggcgtc cggccggttt cgtccgcgcc atgaacagcg
     1801 cccgataaaa caacgcccgc ccgtcgcggc gggccaacag acgatgcctt accgccccgg
     1861 agggggtctt acatgcgtgc tgatgacaaa ggcgagcgcc gctcggattc gcggcctcat
     1921 gctctcggcg actccttatc cggcaccaat ctcgaacgcg cgggcgacca caatcagcgc
     1981 gtcaccctgc aagccgtgcg cgcggcgggc gcgccgatca cccgcgccga aatcgccgag
     2041 ctgaccgggc tgaccgcccc ggccatcgcc aacatcacca agcgcctgct gggcgacggc
     2101 atgatcctca aggccgggcg gttgaccggc gggcgcggcc agccggcgac caagctggtg
     2161 gtcaacccgg acggcgcctt ttccatcggg ctgaacatcg accgcgacca tatcgccatc
     2221 gtcgcgctgg acttcctcgg caatgtccgc ttccgcacat gccgcgaagt ccatttcgcc
     2281 atgccgcagg aggtcatcgc cttcttccgc gagcaggtgg acatgatcat cgcgcagaag
     2341 gcgatcaata tcgagcggct gatcggcatc ggcatcggga ttcccgacga tctcggccgc
     2401 gtgccggtcg ccaaccgccc ggacgcctat cgtatctggt cggagatcga cgtggcggcg
     2461 ctgttcgccg agattctgcc cacgcccgtc cccacgccgg tctatgtcga gaacgacgcg
     2521 gcggcggcct cgatcggcga gatgcagttc ggccacggcc tgcaaagccg cagcttcatc
     2581 tacaccatca tcagcgcggg cctcggcggc gggctggtca tcgacggcca ctattatcgc
     2641 ggcatgaacg gccaatcggg cgagatcggc ttcctgccgg tgcgcacgga cagcggcgag
     2701 gaaaagtcgc tggaggatct ggtttcgctc tatgcgctgt acgacgacct gcgcaagggc
     2761 ggcttcgacg tctcgacgcc ggacgatctg gagcagttga gcgaagccgg acaggcgcgc
     2821 gtcgaggcgt ggctggaccg cgccgccgaa catctgaccc agccgttcct ggtcatgaac
     2881 tgcgccatcg acccggaggc gcactatatc ggcggtcgcc tgcccgccgc ct
//
LOCUS       DASVLL010000098         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_5573, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000098 DASVLL010000000
VERSION     DASVLL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5573"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VF402_01610"
     CDS             <1..221
                     /locus_tag="VF402_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799014.1"
                     /translation="NEQMLLSMAFGGGGMGMGQGLTGGMTGGLMGGLSGGMAPGDLLL
                     SAQLMSAFGPARDDDDQDAGSAFSGLLG"
     gene            complement(210..2009)
                     /locus_tag="VF402_01615"
     CDS             complement(210..2009)
                     /locus_tag="VF402_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7799015.1"
                     /translation="MTASAPTSAAAPSPLSRDEVSLDYGLKAQSRLALYAAGFFGIGL
                     PLLLWVAHLSLPGWLLGLWLAAFTLGWSAFLALRRQVRRLEPQGEAADRADLRRRLHR
                     HCAASGVWVAIQIGMCLSAALYGARPDMLLLLCAGSAAGVIFFSAPVLIGLLVFGVLA
                     AAAPLLALMLVPHDSQMTQIMSGGLALTFAMAFTLNRHMQEHYLLEHDRLALARERES
                     ARAEAQAQGQARIALMETLSREIRTGLRGVEQNLLHSLTHLTRAPAPRQYVDAALGEI
                     GHLQTMLTTTLDNDTAAAGRIELDTAPLDIGLICRRLCAQFAGLAQGKGLGLSCNLDA
                     LPARGAALGDEHRVEQALAHLLSNALLYTAQGRVELKVLALSDGFLRLEVVDSGPGLS
                     PGELAQAFRPHVRIARTSAGTSGAGLGLSLSRSLAELMGGRAGGESTRDVGSKFWLDL
                     PFDPQAVPPASGDVPTDAAASPAPSLRVLLIARDGLSALELRDMLERLGHKCLSATTR
                     ERGLSLAKKAEIDACVISDGRFDALDDDAGRARLSAFVAALNAGQPAPPRLIALLPAG
                     DQAEALQALGITALILPPSPESLSRALTRVSAQ"
     gene            2193..3350
                     /locus_tag="VF402_01620"
     CDS             2193..3350
                     /locus_tag="VF402_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX7799016.1"
                     /translation="MNAPFAPTQARAPLSEADISRLVASGNADDRAVAAHKVCRAMER
                     RTLSAAERDAAQDIIRLLAGDAAELVRRALAVTLRTSDLLPHDVAVKLAQDALSVAVP
                     LISHSPLFSDDDLTAMIRSGGPVRQVAVAKRETLSETVTTALAESAVEEAVVVACAND
                     NARFTEAGMGRVLDRFGESEVVHSVLIHRERLPVSVSERLIHIVSSALREQLVAQHAI
                     RPETALEIATATRERATLDLADQTGRNRDPEALAKHLAANGRLNASLLLRALARGHMS
                     FFEHALAELAGVPHARTALMVHDAGPLGLRAVYDRAGLPARLFPTFRVAVDTWRALSS
                     EAGHLDAGHFQTLMIERFLTQAPYAPREDLAYLYERLDRRLSAEATPLKAA"
     gene            complement(3416..3910)
                     /locus_tag="VF402_01625"
     CDS             complement(3416..3910)
                     /locus_tag="VF402_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX7799017.1"
                     /translation="MSEKAQVIQVPNTLRAKVGGRMGAIDKDAIAKAEAALADLSSQF
                     GDWLLEEIKKLEDVQALIKSEGLNPENSDQLFYRAHDLKGLGTTYGFPLISRIAGSLC
                     KMLDEPEKRAQAPRMLVDAHLDAIRAIVRDQIKDEGHPVGKILSETLEARVKEHLGKI
                     GMAG"
     gene            complement(4087..4845)
                     /locus_tag="VF402_01630"
     CDS             complement(4087..4845)
                     /locus_tag="VF402_01630"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX7799018.1"
                     /translation="MSLRLDFAASDRPEAQIACARLKARYGASAKPDVVVALGGDGFL
                     LQTVHKYLRDQTPIFGMNRGSVGFLLNDYAEDDLIERILKSESTLISPLNMTARTRSG
                     ETRKALAFNEVSLLRQSHQGAKLRILIDGKARLDELMCDGVMVATPAGSTAYNLSAHG
                     PIIPLTAKALALTPISAFRPRRWRGAILPHTAEVRIETLEADKRPISAAADTFDVRDV
                     ISVDIAEAEDLKATLLFDAGNGYDERVLAEQFAS"
BASE COUNT          741 a         1650 c         1764 g          746 t
ORIGIN      
        1 gcaacgagca gatgctgctg tcgatggcgt tcggcggcgg cggtatgggc atggggcagg
       61 ggctgacagg gggcatgact gggggcctga tggggggcct gtcgggcggc atggcgccgg
      121 gcgatcttct gctgagcgcg caactgatgt cggccttcgg accggccagg gacgacgacg
      181 atcaggacgc cgggtccgcc ttttccggcc tactgggctg acacccgcgt cagggcgcgg
      241 ctcaggcttt ccggcgacgg cggcaggatc agcgccgtga tccccagcgc ctgcaaggct
      301 tcggcctgat cgcccgcggg cagcagggcg atgaggcgcg gcggcgccgg ttggcccgcg
      361 ttcagcgccg cgacgaaggc gctcaggcgc gcgcgtccgg cgtcgtcgtc cagtgcgtcg
      421 aagcgtccgt cgctgatcac gcaggcgtcg atctcggcct ttttcgccag cgacaggccg
      481 cgttcgcgcg tcgtcgcgct caggcatttg tgccccagcc gctccagcat gtcgcgcagc
      541 tccagcgcgc tcaggccgtc cctggcgatc agcagcacgc gcagggacgg cgccggagac
      601 gccgcggcgt cggtcgggac atccccggac gcgggcggga ccgcctgcgg atcgaacggc
      661 aggtcgagcc agaatttcga gccgacgtcg cgcgtgcttt cgccgcccgc gcgtccgccc
      721 atcagttcgg ccagcgaccg cgacaggctc agacccagac cggcgccgga cgtgcccgcc
      781 gaggtgcgcg cgatgcggac gtgcgggcgg aaggcctgcg ccagttcgcc ggggctgagg
      841 ccggggccgg aatcgacgac ttcgaggcgc agaaagccgt cgctgagggc cagaaccttc
      901 agctcgacgc ggccctgcgc ggtgtagagc agggcgttgg acagaaggtg cgccagcgcc
      961 tgctcgacgc gatgctcgtc gcccagcgcc gcgccgcgcg cgggcagggc gtcgagattg
     1021 cagctcaggc cgaggccctt gccctgcgcc aggcccgcga actgcgcgca caggcggcgg
     1081 cagatcaggc cgatgtccag cggcgcggtg tccagctcga tgcggcccgc cgccgccgtg
     1141 tcgttgtcca gcgtcgtggt cagcatggtt tgcagatggc cgatctcgcc cagcgcggcg
     1201 tcgacatatt gccgcggcgc cggggcgcgc gtcaggtggg tcaggctgtg cagcaggttc
     1261 tgctcgacgc cgcgcaggcc ggtccgaatc tcgcgcgaca gggtctccat cagcgcgatg
     1321 cgcgcctgac cctgcgcctg cgcctcggcg cgcgcgcttt cgcgctcccg cgccagcgcc
     1381 agccggtcgt gttcgagcag gtaatgctcc tgcatatgac ggttgagcgt gaaggccatc
     1441 gcgaaggtca gggccagccc gcccgacatg atctgcgtca tctgcgaatc gtgcgggacc
     1501 agcatcagcg ccagcagcgg cgcggcggcg gccagcacgc cgaagaccag caggccgatc
     1561 agcaccggcg ccgaaaagaa gatcaccccg gcggcgctgc ccgcgcacag cagcaacagc
     1621 atgtccggcc gcgcgccgta aagcgccgcg ctcaggcaca tgccgatctg gatcgccacc
     1681 cagacgccgc tggccgcgca atgccggtgc aggcggcggc gcagatcggc gcggtcggcg
     1741 gcctcgccct gcggctccag ccgccggacc tgacggcgca gggccagaaa ggccgaccag
     1801 cccagcgtga acgccgccag ccacaggccg agcagccagc cgggcaggct cagatgcgcg
     1861 acccagagca gcaggggcag gccgatgccg aaaaaaccgg cggcgtacag ggccagccgc
     1921 gactgggcct tcaggccgta atcgagcgac acctcgtcgc gggacagagg cgaaggggcg
     1981 gcggcggacg tcggcgcgga tgcggtcatg gcccccagtc ttaggcggca agcgttgctt
     2041 tcctgttacc cgcgcgggcg gaacggcggc gcgggcgtaa aaaaacgatg cggaaatgcg
     2101 cgcggcgcgg gcgcggcgcg caaaagtaaa ggattcgtta gtcatccggc gcgagacttc
     2161 ggcattacgc ggcttttgaa ggattcgctg ccatgaacgc gccgttcgcc cctacccagg
     2221 cccgcgcccc gctgagcgag gccgacatct cgcggctcgt cgccagcggc aacgccgacg
     2281 atcgcgccgt cgccgcgcac aaggtctgcc gcgccatgga gcgccgcacg ctgagcgcgg
     2341 ccgaacgcga cgccgcgcag gacatcatcc gcctgctggc cggggacgcc gccgaactgg
     2401 tgcggcgcgc gctggccgtg accctgcgca cctcggacct gctgccgcac gacgtcgccg
     2461 tgaagctggc gcaggacgcc ctgagcgtgg ccgtgccgct gatcagccat tcgccgctgt
     2521 tcagcgacga cgacctgacc gcgatgatcc gctccggcgg gccggtgcgt caggtcgcgg
     2581 tcgccaagcg cgagactctg tcggaaaccg tcaccacggc cctggccgaa agcgcggtcg
     2641 aggaggcggt ggtcgtggcg tgcgccaacg acaatgcgcg cttcaccgag gcgggcatgg
     2701 gccgcgtgct ggaccgcttc ggcgagtccg aggtggtgca cagcgtgctg atccaccgcg
     2761 agcgcctgcc cgtatcggtg tccgagcggc tgatccacat cgtgtcgtcg gccctgcgtg
     2821 agcaactggt ggcgcagcac gccatccggc cggagacggc gctggagatc gccacggcga
     2881 cgcgggagcg cgcgacgctg gacctggccg accagacggg ccgcaaccgc gacccggagg
     2941 cgctggccaa gcatctggcg gcgaacgggc ggctgaacgc ctcgctgctg ctgcgggcgc
     3001 tggcgcgcgg ccatatgagc ttcttcgaac atgcgctggc cgaactggcg ggggtgccgc
     3061 acgcgcgcac cgcgctgatg gtgcacgacg ccgggccgct ggggctgcgc gccgtttacg
     3121 accgcgccgg cctgcccgcg cggctgttcc cgaccttccg cgtggcggtc gacacctggc
     3181 gggcgctgtc ctcggaggcc gggcatctgg acgccggcca tttccagacc ctgatgatcg
     3241 agcgcttcct gacccaggcg ccctacgcgc cgcgcgagga tctggcctat ctctacgaac
     3301 ggctggaccg ccgtttaagc gccgaggcga cgccgctcaa ggccgcctaa acggaccgga
     3361 catgaaaaaa gccgccccac cgggcggctt ttttgtttcg tataaaggcg gccgtttatc
     3421 cggccatgcc gatcttgccg aggtgttcct tcacgcgcgc ttcgagggtt tcggacagga
     3481 tcttgccgac cgggtggcct tcgtccttga tctggtcgcg gacgatggcg cggatggcgt
     3541 cgaggtgcgc gtcgaccagc atgcgcggcg cctgcgcgcg cttttccggc tcgtccagca
     3601 tcttgcacag cgaaccggcg atgcggctga tcagcggaaa gccgtaggtc gtgccaaggc
     3661 ccttgaggtc gtgggcgcga tagaacaact gatcgctgtt ttccgggttc aggccctcgc
     3721 tcttgatcag cgcctgcacg tcctccagct tcttgatctc ctccagcagc cagtcgccga
     3781 actggctgga caggtcggcc agcgcggcct cggccttggc gatggcgtcc ttgtcgatcg
     3841 cgcccatgcg tccgccgacc ttggcgcgca gggtgttggg aacctgaatg acctgggctt
     3901 tttccgacat gggaactcct gaaaagccat gactactatg gtaaggttaa gaccgggatg
     3961 tttaagaagc gctaatgcgc ggcgtttttt agagcgttcc cctcaaaagc gactccgctt
     4021 ccgggataag gaagggcggc aaaacaaaga tttagcgcgc cggtccgatt ccattggatc
     4081 ggcgcgctaa gaggcgaact gctccgccag cacgcgctcg tcgtagccgt tgcccgcgtc
     4141 gaacagcagg gtggccttga gatcctcggc ctcggcgatg tcgacgctga tcacgtcgcg
     4201 cacgtcgaag gtgtcggcgg cggcgctgat cgggcgcttg tcggcctcca gcgtctcgat
     4261 gcgcacctcg gcggtgtgcg gcaggatggc gccgcgccag cgccgggggc ggaaggcgct
     4321 gatcggagtc agggccaggg ccttggcggt cagcggaatg atcggcccgt gcgccgacag
     4381 gttgtaggcg gtcgaaccgg cgggcgtggc gaccatgacg ccgtcgcaca tcagctcgtc
     4441 gaggcgcgcc ttgccgtcga tcaggatgcg cagcttggcg ccctgatgcg actggcgcag
     4501 cagggacacc tcgttgaagg ccagcgcctt gcgcgtctcg cccgaacggg tgcgcgcggt
     4561 catgttgagc gggctgatca gcgtgctttc gctcttgagg atgcgctcga tcaggtcgtc
     4621 ctcggcgtag tcgttgagca ggaaaccgac gctgccgcga ttcatgccga agatcggcgt
     4681 ctggtcgcgc aggtatttat gcaccgtttg cagcaggaag ccgtcgccgc ccagcgccac
     4741 caccacgtcc ggtttggccg acgcgccgta ccgggctttc agccgcgcgc aggcgatctg
     4801 ggcttcgggg cggtcggagg cggcgaaatc gaggcgaagg gacattccgg cggatcgttc
     4861 tggctgtgaa gcgtctatgt aagggctttg ccgcgttccg t
//
LOCUS       DASVLL010000099         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_327712,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000099 DASVLL010000000
VERSION     DASVLL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327712"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF402_01635"
     CDS             <1..457
                     /locus_tag="VF402_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7799019.1"
                     /translation="KDNNASCALGPFIRMFDDGFTIDDVRGAVLDLKIDGPEGYELVG
                     RSTMSEISRDPLELVRQAMSEHHYPDGFVLMLGTLFAPTQDRDVPGRGFTHKVGDVVK
                     VATPKLGCLENRVTTSKDARHWTFGITALMQNLAARGLLGAPQGARKAG"
     gene            501..1238
                     /locus_tag="VF402_01640"
     CDS             501..1238
                     /locus_tag="VF402_01640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7799020.1"
                     /translation="MSSAIYPSLKGRKVLITGGGSGIGAGLVAGFAKQGAQVHFIDLV
                     DEISASLTDNRPVFHKCDLTDTPALEKVLANILADGPVNILVNNAARDDRHSLEEITS
                     EFFDRNIGVNLKHLVMCTRVVAPAMKAAGGGVILNFGSISWHLGLPDLALYETAKAGI
                     EGFTRAMARELGRDNIRCNAIIPGNVKTPRQMKWYTPEGEKQIVDAQCLDGRLLPENV
                     ASLALFLASDDASLLTAHSYWIDAGWR"
     gene            1354..>1854
                     /locus_tag="VF402_01645"
     CDS             1354..>1854
                     /locus_tag="VF402_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7799021.1"
                     /translation="MTDLFQPVCVWDLKATLGEGPVWSAHDGAVWFVDIKQSQVHRYD
                     AATGAHRSYAAPDPCGFLAPKQGGGFIVGCKSGLYDFDPLSGDFAFRAAIEPGFPDNR
                     LNDGYVDHQGRLWFGSMHDPEERPTGKLYRLDARGLKLMDDDYVITNGPAVSPDGKTL
                     YHNDTLK"
BASE COUNT          337 a          604 c          596 g          317 t
ORIGIN      
        1 caaggacaac aacgcctcgt gcgcgctggg gccgttcatc cgcatgttcg acgacggctt
       61 caccatcgac gacgtgcgcg gcgccgtgct ggacctcaag atcgacgggc cggaaggcta
      121 tgagctggtc gggcgctcca ccatgagcga gatcagccgc gatccgctgg agctggtgcg
      181 tcaggcgatg agcgagcatc actatcccga cggcttcgtg ctgatgctcg gcacgctgtt
      241 cgcgccgacg caggatcgcg atgtgccggg ccggggcttc acgcacaagg tcggcgacgt
      301 ggtcaaggtg gcgacgccga aactgggctg tctggaaaac cgcgtcacca cctcgaagga
      361 cgcgcgccac tggaccttcg gcatcaccgc gctgatgcag aatctggccg cgcgcggctt
      421 gctgggggcg ccgcagggcg cgcgcaaggc cggctgactg gcccaaacca taaaattgag
      481 aagaaaagcg agtttttttg atgtcgtctg ccatttatcc cagcctgaag ggccgcaagg
      541 tgctgatcac cggcggcggt tcgggcatcg gcgccggtct ggtcgcgggc ttcgcgaagc
      601 agggcgcgca ggtgcatttc atcgatctgg tcgatgaaat ctccgcgtcc ctgaccgaca
      661 accgcccggt ctttcacaag tgcgacctga cggacacgcc cgcgctggaa aaggtgctgg
      721 cgaacattct ggcggacggt ccggtcaaca tcctggtcaa caacgccgcg cgcgacgacc
      781 ggcattcgct ggaagagatc acctcggaat tcttcgacag gaatatcggc gtcaacctca
      841 agcatctggt catgtgcacg cgcgtcgtgg ccccggccat gaaggcggcg ggcggcggcg
      901 tgatcctgaa cttcggttcg atctcgtggc acctcggcct gcccgacctg gcgctgtacg
      961 aaaccgccaa ggcgggcatc gaaggcttca cgcgggcgat ggcgcgcgaa ctgggccgtg
     1021 acaacatccg ttgcaacgcc atcattcccg gcaatgtgaa aacgccgcgc cagatgaagt
     1081 ggtacactcc cgaaggcgaa aagcagatcg tcgacgccca gtgcctcgac ggccgcctgc
     1141 tgccggagaa cgtcgcctcg ctggccctgt tcctggcctc ggacgacgcc agcctgttga
     1201 ccgcgcacag ctactggatc gacgccggct ggcggtaata ccccctttat ccctcctcgt
     1261 ttacggggag gggggaccgc agatgctcag gaagtgaagc cttggcatgc ttccgggcgg
     1321 tgggaggggg cctctttggc aggagttgtg ttcatgaccg atcttttcca acccgtctgc
     1381 gtctgggacc tcaaggccac gctgggcgaa gggccggtct ggagcgcgca tgacggcgcc
     1441 gtctggttcg tcgacatcaa gcaaagtcag gtccaccgct acgacgccgc caccggcgct
     1501 caccgtagct acgccgcgcc cgacccgtgc ggtttcctcg cgccgaaaca gggcggcggc
     1561 ttcatcgtcg gctgcaagag cgggctttac gatttcgatc cgctcagcgg cgatttcgcc
     1621 ttccgcgccg ccatcgagcc gggctttccc gacaaccgcc tcaatgacgg ctatgtcgat
     1681 catcagggcc gcctgtggtt cggctcgatg cacgatccgg aggagcggcc caccggcaag
     1741 ctctaccgcc tcgacgcgcg cggcctgaag ctgatggacg acgattacgt catcaccaac
     1801 ggcccggcgg tcagcccgga cggcaagacc ctctatcaca acgacacgct gaaa
//
LOCUS       DASVLL010000100         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_488926,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000100 DASVLL010000000
VERSION     DASVLL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488926"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..697)
                     /gene="pseF"
                     /locus_tag="VF402_01650"
     CDS             complement(5..697)
                     /gene="pseF"
                     /locus_tag="VF402_01650"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HEX7799022.1"
                     /translation="MSRIAVIPARGGSKRIPRKNIRPFHGRPMIAYPIRAALQSGLFD
                     RIIVSTDDEEIAAVARDSGAETPFVRPPELADDHAGTGEVVVHAIRWLIDHGQPVDAC
                     CCIYATAPFLTPERLREGWRALQTRGKAFAFSATSYAFPIQRAIRLTPGGGVEMFWPQ
                     HRTTRSQDLEPAYHDAAQFYWGLGTAWLSGVDAFSEAGAAVILPRAEVVDIDTPEDWE
                     VAEQAYRLLKGI"
     gene            complement(694..1614)
                     /locus_tag="VF402_01655"
     CDS             complement(694..1614)
                     /locus_tag="VF402_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HEX7799023.1"
                     /translation="MTSFGERLKTRVPDWPRFLARARDDRRNIAYDLLPLLDLKGREA
                     VARALRRLKPHTRPFAPSDRARDLDAQLRRDGVTAPQPPLPDHVLADLVAWFKTQPCH
                     DRYRPHLGEFSWDAPASDETNIGYFTTEQVLRAPHLLDLANDRLLLETAELYLGARPV
                     IDNIGCAWHFPGREQAKGVQRFHRDFDCARNLKAFWYLTDVTETSGPHRYVRGSHADP
                     RLDSGKAQTDAAIIAAFGAGNIISITAPAGTWFLEDVYGFHKGQLPIDRPRLLLAVEY
                     NLYPSSLAPRHPIMRNDGRYDRYVNRLFLK"
     gene            complement(1620..1841)
                     /locus_tag="VF402_01660"
     CDS             complement(1620..1841)
                     /locus_tag="VF402_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7799024.1"
                     /translation="MDKPAFFNAVAEILEADPSGLTGAERIEDVGNWDSLSVISFVAM
                     VDSDLNQIVDGEKLKDAKTLNDLAALVGL"
     gene            1978..>2564
                     /locus_tag="VF402_01665"
     CDS             1978..>2564
                     /locus_tag="VF402_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEX7799025.1"
                     /translation="MTEARISGVALRGMRACVPALTRDLAGEGLIPDEAERERLIKSI
                     GVRSRHIAPPGVLTSDLCRKAADGLLDELGWARDTIDVLIRVTQSADYVIPATACALQ
                     DRLGLGSCLAFDVNLGCSGYAYGLWTAASLLKTLQVGRPARALVLAGDVSTSKLLEGD
                     RATIPLFGDAGSATALEIDPDAQDDWFGVFGTDGSG"
BASE COUNT          413 a          813 c          914 g          424 t
ORIGIN      
        1 gcggtcaaat ccctttgagc agccggtacg cctgctcggc gacctcccag tcctcgggcg
       61 tgtcgatgtc gaccacctcg gcgcgcggca ggatgacggc ggcgcccgcc tcggaaaagg
      121 cgtccacgcc cgacagccac gccgtgccca gcccccagta gaactgcgcc gcgtcgtgat
      181 aggcgggctc cagatcctgc gagcgcgtcg tcctgtgctg tggccagaac atctccacac
      241 cgccgcctgg ggtcagccgg atcgcgcgct ggatcggaaa ggcgtaggag gtcgcggaaa
      301 aggcgaaggc cttgccgcgc gtctgcagcg cccgccaacc ttcgcgcagg cgttccggcg
      361 tcagaaacgg cgcggtggcg tagatgcagc agcaggcatc gaccggctgg ccgtgatcga
      421 tcagccagcg gatcgcatgc acgacgacct cgccggtgcc cgcatggtcg tcggccagct
      481 ccggcgggcg cacgaacggc gtttccgcgc cggagtcgcg cgcgacggcg gcgatctcct
      541 cgtcgtcggt cgagacgatg atccggtcga acaggccgct ttgcaacgcc gcgcggattg
      601 gataggcgat catcggccgg ccgtggaacg gccggatgtt cttgcgcgga atgcgtttgg
      661 agccgccgcg cgcggggatg acggcgatgc ggctcatttc aggaacagcc ggttgacgta
      721 gcggtcatag cgcccgtcgt tccgcatgat cggatggcgc ggcgccagcg acgacggata
      781 gagattatat tcgaccgcca gcaacaggcg cggccggtcg atgggcaact ggcccttgtg
      841 aaagccatag acgtcttcaa ggaaccatgt tcccgccggg gccgtgatgc tgatgatgtt
      901 cccggcgccg aaggcggcga tgatggctgc gtcggtctgg gccttgccgc tgtcgaggcg
      961 cgggtcggcg tgcgagccgc gcacgtatct gtgcgggccg gaggtttcgg tcacatcggt
     1021 cagataccag aaggccttga gattgcgcgc gcagtcgaaa tcgcggtgaa agcgctgcac
     1081 gcccttggcc tgttcgcggc ccggaaagtg ccaggcgcag ccgatattgt cgatgacggg
     1141 ccgcgcgccg agataaagct cggcggtttc gagcagcagc cggtcattgg ccagatcgag
     1201 cagatgcggc gcgcgcagca cctgctcggt ggtgaaatag ccgatattgg tctcgtccga
     1261 agccggcgcg tcccacgaaa attcgccgag gtgcgggcga tagcggtcgt ggcacggctg
     1321 ggtcttaaac caggcgacca gatcggccag cacatgatcg ggcagcggcg gctgcggcgc
     1381 ggtgacgccg tcgcggcgca actgcgcgtc cagatcgcgc gccctgtccg agggcgcgaa
     1441 ggggcgcgta tgcggcttga ggcggcgcaa ggcgcgcgcg acggcctcgc ggcccttcag
     1501 gtccagcagc ggcagcaggt cgtaggcgat gttgcgccgg tcgtcacggg cgcgcgccag
     1561 aaagcgcggc cagtcgggca cacgggtttt cagccgctcg ccgaagctcg tcatgcggtc
     1621 taaaggccga ccagcgcggc cagatcgttg agcgttttgg cgtctttcag cttttcgccg
     1681 tcgacgatct ggttcaggtc ggaatcgacc atggcgacga aggagatcac cgacagggaa
     1741 tcccagttgc cgacgtcctc gatcctttcc gcgccggtca ggccggacgg atcggcttcg
     1801 agaatttccg cgacggcgtt gaaaaaggcg ggcttgtcca tcggtgactc ctgatgcggt
     1861 gttttgccgc cagcaagcgc cgattccgtc aatattccct taaaaccttg ctgccagcct
     1921 tgcgtcctga aagggcgaat cgccctaagg tgcgcggcgt aaaacgggac gacaatcatg
     1981 accgaagcca ggatcagcgg cgtagccctt aggggcatgc gcgcctgcgt gcccgccctg
     2041 acgcgcgatc tggccgggga gggcctgatc ccggacgagg ccgagcgcga gcggctgatc
     2101 aaatccatcg gtgtgcgctc gcgccatatc gcgccgccgg gcgtcctgac ctccgacctg
     2161 tgccgcaagg cggcggacgg gctgctcgat gaactgggct gggcgcgcga caccatcgac
     2221 gtgctgatca gggtgacgca gagcgccgac tatgtgatcc cggcgaccgc ctgcgccttg
     2281 caggaccggc tggggctggg gtcgtgcctg gccttcgacg tcaatctcgg ctgttccggc
     2341 tatgcctacg ggctgtggac ggcggcgtcg ctgctgaaga ccttgcaggt gggccgtccg
     2401 gcgcgcgccc tggtgctggc gggcgatgtc tcgacctcca agctgctgga aggcgaccgc
     2461 gcgacgatcc cgctgttcgg cgacgccggt tccgccacgg cgctggagat cgatccggat
     2521 gcgcaggacg actggttcgg cgtgttcgga accgatggct ccgg
//
LOCUS       DASVLL010000101         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_327847,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000101 DASVLL010000000
VERSION     DASVLL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327847"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF402_01670"
     CDS             <1..543
                     /locus_tag="VF402_01670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7799026.1"
                     /translation="PVTGLDVPPATARVEIDTGEGPIVLELDGRDAPQSTANFLHYVD
                     TGKLDGGTFYRAVRAGKSGFVQFNSGARTYPPIPHEPTTRTHLSHTDGTISTARYAVG
                     TASNEFTIIVGDMTYLDAGGGATPDKQGYAAFGHVVSGMETVRKILRAPLSKAKPAPG
                     DWPDETLARPVVIVKAYRLK"
     gene            complement(565..1290)
                     /locus_tag="VF402_01675"
     CDS             complement(565..1290)
                     /locus_tag="VF402_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX7799027.1"
                     /translation="MQINSSAGLSLSQLLSQLTSSSTSGSSATTSTDASSSTTTTSTS
                     AASFSENLDYFLSALSSKVLDSLMSLQTQSASAGVSSLSGGGHHHHHMGGGVGDSSGE
                     NGPPSLSDIDSNGDGAISQSEMEAYGQSVMGDSDTSKADALFSQMDTNGDGTVSADEK
                     SAFDQQMAAQGSQGPDGPPPGPPPTSGDGDSTSSGDSSSSSSNELASLMQQFIQALQS
                     YSQAGASSGTADTATTSSVAVDA"
     gene            1584..2267
                     /locus_tag="VF402_01680"
     CDS             1584..2267
                     /locus_tag="VF402_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799028.1"
                     /translation="MRPYPLLLAAAALALHVAPAVAQDYLDTGAGQQVFFSPSGQPFR
                     AAPDKPYPSVDWFNAADANHDGKISHDEFVADAMRFFDMLDVNHDGYINSPENTRYET
                     QIAPEIQRVDPRIQQPKNITHNDDDAATQDMPNGGRYLKQIVGASQYGLIDEPQPIRG
                     ADANFDFRVSKAEWLTATNQRFAILGRNGDGYITPDELPRTPAQLAQESGKNDRKDDH
                     RRKKHSGNW"
     gene            2409..>2846
                     /locus_tag="VF402_01685"
     CDS             2409..>2846
                     /locus_tag="VF402_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX7799029.1"
                     /translation="MFKKVHRLGLFGLGAFGRLIVKHLSPYFDIYAYDPSPHARAFAK
                     RHNVTLASLEEVAACRVVLLAAPVRALKSLAESIAPHVHPRGLIIDVGSVKVKPAQWL
                     RDALPPSVYILCTHPLFGPQSARKGIHDLEVVVCPVRIRRLAPI"
BASE COUNT          530 a          972 c          836 g          508 t
ORIGIN      
        1 cccgtcaccg ggctggacgt tccgcccgcc acggcgcgcg tcgagatcga caccggcgaa
       61 ggcccgatcg tgctggagct ggacggccgg gacgcgccgc aaagcacggc caacttcctg
      121 cactatgtcg atacgggcaa gctggacggc ggaaccttct accgcgccgt gcgcgccgga
      181 aaaagcggct tcgtgcagtt caacagcggc gcgcgcacct atccgccgat cccgcacgag
      241 ccgacgacac ggacccacct gtcgcacacc gacggcacga tctcgaccgc gcgctacgcc
      301 gtcggcacgg cgtcgaacga gtttaccatc atcgtcggcg acatgaccta tctcgacgcg
      361 ggcggcggcg ccacgccgga taaacagggc tacgccgcct tcggccatgt cgtttcgggc
      421 atggagacgg tgcgcaaaat cctgcgcgcg ccgctgtcga aggcgaaacc cgcccccggc
      481 gactggccgg acgaaaccct ggcccggccc gtcgtcatcg tgaaggcgta caggctgaaa
      541 taggcggctc ttttgggatg cgcttcaggc gtccaccgcc acgctggagg tcgtcgccgt
      601 gtcggcagtc ccggaggaag cgcccgcctg cgagtaggat tgcagcgcct gaatgaactg
      661 ctgcatcagg ctggccagtt cgttcgagga ggacgaggac gagtcgccgg acgaggtcga
      721 atcgccgtcg ccggacgtcg gcggcggacc cggcggcggg ccatccggtc cttgcgaccc
      781 ctgcgcggcc atctgctgat cgaaggcgct cttttcgtcc gccgacaccg ttccgtcgcc
      841 gttcgtatcc atctggctga acaaagcgtc ggctttcgac gtatcgctgt cgcccatgac
      901 cgactggccg taggcttcca tctcgctctg gctgatcgcg ccgtcgccgt tggaatcgat
      961 gtcgctgagc gagggcggcc cgttttcgcc gctgctgtcg ccgaccccgc cgcccatgtg
     1021 atgatggtgg tggccgccgc cgctcaggct gctgaccccc gccgaggccg actgcgtttg
     1081 cagcgacatc aggctgtcga gcaccttcga cgacagggcg ctgaggaagt aatcgaggtt
     1141 ttcgctgaag ctggccgcgc ttgtgctggt cgtcgtcgtg gacgaggacg cgtcggtcga
     1201 cgtcgtcgcg gaactgccgg acgtcgaact cgacgtcaac tgagacaaca actggctcag
     1261 actcagtccc gccgaactgt tgatttgcat gagaccctcc tgatagaacc gccgggagag
     1321 tggtcccgga caatcatcag aggctctttt taaattaaca agatactaat ttaatgaaga
     1381 tttcagtttt gaaacgcaat cgtcaaattt aaatcgaatt acgcagcgcc attttcattg
     1441 catataacat cgattacaca aacacaacac ctttttactg taggcattat ttgcccggca
     1501 agagctgccg cccgccgata gcctcttgcc tgcccggcga ggcgcggtta agttgccgtc
     1561 cttccttcga gagagacccc ctcatgcgcc cgtatccgct tttgctcgcc gccgccgccc
     1621 tggccttaca cgtcgcgccc gccgtcgcgc aggactatct cgacaccggc gcgggccagc
     1681 aggtcttctt ctcgccgtcg ggccagccct tccgcgccgc gccggacaag ccctacccca
     1741 gcgtggactg gttcaacgcc gccgacgcca accacgacgg caagatcagc cacgacgagt
     1801 tcgtcgccga cgcgatgcgc tttttcgaca tgctggacgt caatcacgac ggctatatca
     1861 acagcccgga aaacacccgc tacgagaccc agatcgcgcc ggagatccag cgcgtcgatc
     1921 cgcgcatcca gcagcccaag aacatcacgc acaacgatga cgacgccgcc acccaggaca
     1981 tgcccaatgg cggccgctac ctgaaacaga tcgtcggcgc ctcgcaatac ggcctgatcg
     2041 acgaaccgca gccgatccgc ggcgccgacg ccaatttcga cttccgcgtc tcgaaggccg
     2101 aatggctgac cgccaccaat cagcgcttcg ccatcctggg ccgcaacggc gacggctata
     2161 tcacgcccga cgaactgccg cgcaccccgg cccaattggc gcaggagtcc ggcaaaaatg
     2221 acaggaagga cgaccatcgc cggaaaaaac attccgggaa ttggtgattt cggggataat
     2281 ccggccaaaa ctgtttgttg cagcgcggaa accgaccttg cgtgatcaca aaagtgcaat
     2341 ttccatctcg ccagcccgaa gcttttgtgc tactgcccgc gtttctttgt taacggaaac
     2401 ggcgctgcgt gttcaagaaa gtacacagac tgggcctttt cggtctcggc gcgttcggcc
     2461 gtctgatcgt caagcacctg tcgccttatt tcgacatcta tgcttacgat cccagcccgc
     2521 acgcgcgggc cttcgccaag cggcacaatg tcaccctggc cagccttgag gaggtcgcgg
     2581 cctgccgcgt cgtgctgctg gccgcgccgg tgcgcgcgct gaaatccctg gcggagagca
     2641 tcgccccgca cgtccatccg cgcggcctga tcatcgacgt cggctcggtc aaggtgaagc
     2701 ccgcgcaatg gctgcgcgac gcgctgccgc ccagcgtcta tatcctctgc acccatcctt
     2761 tgttcgggcc gcaatcggcg cgcaagggga ttcacgacct cgaagtcgtg gtctgcccgg
     2821 tgcgcatccg ccgcctggcg ccgatc
//
LOCUS       DASVLL010000102         4672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_167483,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000102 DASVLL010000000
VERSION     DASVLL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167483"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VF402_01690"
     CDS             complement(<1..1542)
                     /locus_tag="VF402_01690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7799030.1"
                     /translation="MAINTGMSKTYTNPLTRDYIALGLALLAFCLHAAFAGRYDVFRD
                     ELYFIVCGRHPAFGYVDQPPLVPLLSAGFYALGHTVWLLRLPAILAAAALVWLAARFA
                     RLLGGGAFAAVMAGLAVTIAPMLMGMATVFSTTAFDPLAWTLIAYLLVRAVQDEDPKA
                     LIWCGVVAGLDFEAKYALAFWAVSLLTGLVLTPQRRIFREKALWLGAALAALIGLPSL
                     IWQAAHGWPFLELAAAARDKNVATPPLAFLLNQLLIMNPFLAPLWIAGVVAPFTIAAL
                     KPVRFLALAFTTCFLLILLTHGKDYYIAAAYPVMFVIGAVAWAHWMKAVKARAALAVW
                     LAPAAAFSAAAAPLALPVLSVPALRAYMQRLPVKPQQQEKSFKGTLLPQVFADQLGWR
                     DFTAQVGASWRQIPPAERARTAIKVDNYGEASALDVYGKAYGLPPALSGHNQYYLWSL
                     SYLSAQHPVNALVVQNHPERLYPYCVHTTIYRTTFSPDAMHSENGKVIAFCQGLKVDL
                     ARIWPD"
     gene            1725..2462
                     /locus_tag="VF402_01695"
     CDS             1725..2462
                     /locus_tag="VF402_01695"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7799031.1"
                     /translation="MIAGFLLTAILVNISSNMIEQPDLPLWEPALWEVSSYIAILIFV
                     PALWRQWRRFHWTRLGVPRFLAAQAAAFLLFSAGHIAVMALIRHAGYALAGETYDFSH
                     GRLALVLIYESRKDFLTFVLTTGMFWAYERLHRTEAPPPRARIEVRADGRILYLSPDE
                     IVSAEAAGNYVELRLTHGKPLLLRATLADYADRLGPDFVRAHRSRLINRAHIREISVT
                     AAGDPRLTLSDGREVAGSRRYRDRLRL"
     gene            2545..>4672
                     /gene="putA"
                     /locus_tag="VF402_01700"
     CDS             2545..>4672
                     /gene="putA"
                     /locus_tag="VF402_01700"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX7799032.1"
                     /translation="MPDAASDFADAFAVKPTDWNRLDDRKYMSDAAICAWLLRAAPLD
                     AAQRARVRADAEALVAATRRAAKKQGVVESFLKEFSLGTAEGLALMCLAEALLRTPDE
                     ATRDALIHEKIASADWASHLGRSDNWFVNASTWGLMLTGRMIDVEAQAKSDLTGYLTH
                     LAGRVGEPVIRKAAAQAIRIMGEQFVLGRTIEAALKRAKAEGYLCSFDMLGEGARNVA
                     DAERHTEAYAGAIAALARVATKGPEADHGVSVKLSALYPRYEAARFDDVMAALYPRLK
                     RLAAQAARADIHFTLDAEESDRLVLSLTVLDRLAREPELGGWTGLGLAVQAYQKRAPE
                     VIAALRVLAQSSGRRLMVRLVKGAYWDTEIKRAQVMGREDYPVHTTRAATDLSYLVCA
                     RMLIDAAPHLYAQFATHNAHSLAAIRLMAKEKGVVVESQRLHGMGEALYAEAEKAFGA
                     FPLRAYAPVGGHEDLLPYLVRRLLENGANSSFVNVLLDEKVDVKDVISDPVDKVAAHP
                     YRHARLPLPRDLYGDRLNSHGPDLSVAAARARLAAALRKLDGERREANALIGGRPHST
                     YAAHVFAPADLTRIVGEVHEADPAMIEEAHQRAAAAQPAWNALGGDGRARVLEAMADA
                     LEAGRESLCALMVREAGKTWSDAIAETREAADFCRYYAKLAREQFGAPITLTGPVGER
                     NTYGLHGRGVFVCISPWNFPLAIFTGQ"
BASE COUNT          780 a         1495 c         1674 g          723 t
ORIGIN      
        1 gtcgggccag atccgcgcca gatcgacctt gaggccctga cagaaggcga tgaccttgcc
       61 gttttcgctg tgcatggcgt cgggcgaaaa ggtcgtgcgg tagatggtcg tatgcacgca
      121 atagggataa aggcgctccg gatggttctg cacgaccagc gcattgaccg gatgctgagc
      181 gcttaaatag gacaggctcc ataaatagta ctggttatgg ccgctgagcg ccgggggcag
      241 gccgtaggct ttgccgtaaa cgtcgagcgc ggaggcctcg ccgtaattgt cgaccttgat
      301 cgcggtcctg gcgcgttcgg cgggcggaat ttgccgccat gaggcgccga cctgagcggt
      361 gaagtcgcgc cagcccaact ggtcggcgaa gacctgtggc agcagcgtgc ccttgaagct
      421 cttttcctgc tgctgcggct tgacgggcag gcgctgcatg taggcgcgca gggccgggac
      481 gctgaggacg ggcagggcca gaggcgcggc ggcggcggaa aaggcggcgg ccggcgccag
      541 ccagacggcc agcgccgcgc gggccttgac ggccttcatc cagtgcgccc acgccacggc
      601 gccgatgacg aacatcaccg ggtaggccgc cgcgatgtaa tagtccttgc cgtgcgtcag
      661 caggatgagc aggaaacagg tcgtgaaggc gagcgccaga aagcgcacgg gtttcagcgc
      721 cgcgatcgtg aacggcgcga cgacgcccgc gatccacagc ggcgccagaa acgggttcat
      781 gatcagcagt tgattgagca ggaaggcgag tggcggcgtc gccacgttct tgtcgcgcgc
      841 ggcggcggcc agttcgagga acggccagcc gtgcgccgct tgccagatca gggagggcag
      901 gccgatcagg gcggccagcg ccgcgcccag ccacagggcc ttttcgcgga atatccgccg
      961 ctgcggcgtc aggacgaggc cggtcagcag gctgacggcc cagaaggcga gggcgtactt
     1021 ggcctcgaaa tcgagcccgg ccacgacgcc gcaccagatc agcgccttgg gatcttcgtc
     1081 ctgaaccgcc ctgaccagca gataggcgat cagcgtccag gccagcggat cgaaggcggt
     1141 ggtgctgaac accgtggcca tgcccatcag catcggcgcg atcgtcacgg ccagcccggc
     1201 catgacggcg gcgaaggcgc cgccgcccaa gaggcgggcg aagcgcgccg ccagccagac
     1261 cagcgccgcc gccgccagaa tcgcgggcag gcgcaacagc cagacggtgt ggccgagagc
     1321 gtagaaaccg gcggaaagca ggggcaccag cggcggctga tcgacgtagc cgaaggccgg
     1381 atggcggccg cagacgatga aataaagctc gtcacggaac acatcgtaac ggcccgcgaa
     1441 cgccgcgtgc aggcagaagg ccagcagggc cagccccagc gcgatataat cccttgttaa
     1501 gggattcgtg taagtctttg acattccggt gtttatggcc atgttgctgc tcccttgccc
     1561 ggcatcaggc ttgacattcg cgcgttcggg ccgccatctc gaagccgcag gcggcggcgg
     1621 gattgggacg aacggtgatg agcggcgttt catccgggac agacggcggg aaaaacgggg
     1681 caggcggtcg gctgcctcaa agactgccca tacggttgtt cggcgtgatc gccggcttcc
     1741 tgctgaccgc cattctggtc aatatttcct cgaacatgat cgaacagccc gacctgccgc
     1801 tgtgggaacc ggccttgtgg gaagtttcca gctatatcgc catcctgatc ttcgtgcccg
     1861 cgttgtggcg gcaatggcgg cgctttcact ggacgcggct gggggttccg cgctttctgg
     1921 ccgcgcaggc cgcggccttc ctgttgtttt cggcgggcca tatcgcggtc atggccctga
     1981 tccgtcacgc cggttacgcg ctggcgggcg agacttacga tttttcgcac gggcggctgg
     2041 cgctggtgct gatttacgag agccgcaagg atttcctgac cttcgtgctg acgaccggca
     2101 tgttctgggc ctatgagcgg ctgcacagga cggaggcgcc gccgccgcgg gcgcgaatcg
     2161 aggtgcgcgc ggacgggcgc atcctctacc tgtcgccgga cgagatcgtc agcgcggagg
     2221 cggcgggcaa ctatgtcgaa ctgcgcctga cgcacggcaa gccgctgctg ctgcgcgcga
     2281 ccctggccga ctacgcggac cggctggggc cggatttcgt gcgcgcgcac cgttcgcggc
     2341 tgatcaaccg cgcgcatatc cgcgaaatct cggtgacggc ggcgggcgat ccgcgcctga
     2401 ccctgagcga cggccgcgag gtggcgggca gccgccgcta tcgcgatcgc ctcaggctct
     2461 gaacgatcgg agcggcggct atctttgcgc gtcgaacggt agtatcgtcg gtaacgaaaa
     2521 ggtctttgcc cggagcgcta cgccatgccc gacgccgcat ccgatttcgc cgacgccttc
     2581 gccgtgaagc cgacggactg gaatcgtctc gatgatcgca aatacatgtc cgacgcggcc
     2641 atatgcgcat ggctgctgcg cgccgcgccg ctggatgcgg cgcagcgcgc gcgggtaagg
     2701 gcggacgccg aggcgctggt ggccgccacg cgcagggcgg cgaaaaagca gggcgtggtc
     2761 gaaagcttcc tgaaggaatt ttcgctcggc acggcggagg ggctggcgct gatgtgcctg
     2821 gccgaggcgc tgctgcgcac gccggacgag gcgacgcgcg acgcgctgat tcacgagaag
     2881 atcgcctccg ccgactgggc gtcgcatctg ggccggtcgg acaactggtt cgtcaacgcc
     2941 tcgacctggg ggctgatgct caccggccgc atgatcgacg tcgaggcgca ggccaagagc
     3001 gacctcaccg gctatctgac ccatctggcc gggcgcgtcg gcgagccggt gatccgcaag
     3061 gcggcggcgc aggcgatccg catcatgggc gagcagttcg tgctgggccg gaccatcgag
     3121 gcggcgctga aacgcgcgaa ggcggagggc tatctatgtt cgttcgacat gctgggcgaa
     3181 ggcgcgcgca acgtggccga cgccgaacgc cacaccgaag cctatgcggg cgccatcgcc
     3241 gcgctggcca gggtcgcgac caaggggccg gaggcggatc acggcgtttc ggtcaagctg
     3301 tcggcgctct atccgcgcta tgaggcggcg cggttcgacg acgtgatggc ggcgctttat
     3361 ccgcgcctga aacgcctcgc cgctcaggcc gcgcgcgccg acatccattt cacgctcgac
     3421 gccgaggaaa gcgaccggct ggtcctgtcg ctgacggtgc tggaccggct ggcgcgcgag
     3481 ccggagctgg gcggctggac cggcctcggc ctcgccgtgc aggcgtatca gaagcgcgcc
     3541 ccggaagtga tcgccgcctt gcgcgtgctg gcgcaatcga gcgggcggcg gttgatggtc
     3601 cgcctcgtca agggcgccta ttgggacacg gaaatcaagc gcgcgcaggt catgggccgc
     3661 gaggactatc cggtccacac caccagggcg gcgacggacc tcagctatct ggtctgcgcg
     3721 cggatgctga tcgacgcggc gccgcatctt tatgcgcagt tcgccacgca caacgcccat
     3781 tcgctggccg cgatccggct gatggcgaaa gagaagggcg tcgtggtcga atcgcagcgc
     3841 ctgcacggca tgggcgaggc gctgtacgcc gaagccgaaa aggcgttcgg cgcctttccg
     3901 ctgcgcgcct acgcgccggt cggcgggcat gaggatctgc tgccctatct ggtgcgccgc
     3961 ctgctggaga acggcgccaa ttcgtccttc gtcaatgtgc tgctcgacga aaaggtcgat
     4021 gtgaaggacg tgatttccga tccggtcgac aaggtcgcgg cgcatcctta ccgccatgcg
     4081 cgcctgcccc tgccgcgcga cctgtacggc gaccggttga acagccacgg gccggatctt
     4141 tccgtcgcgg cggcgcgcgc gcgccttgcc gccgcgttgc gcaagctgga cggcgagcgc
     4201 agggaggcca acgcgctgat cggcggcagg ccgcattcga cctatgctgc gcacgtgttc
     4261 gcgcccgccg acctgacgcg catcgtcggc gaggtccatg aggccgatcc cgcgatgatc
     4321 gaggaggcgc accaacgcgc cgccgccgcg caaccggcgt ggaacgcgct gggcggcgac
     4381 ggacgggcgc gcgtgctgga ggcgatggcc gacgcgctgg aggccgggcg cgagtccctg
     4441 tgcgcgctga tggtgcgcga ggcgggcaag acgtggagcg acgccatcgc cgaaacgcgc
     4501 gaagccgccg atttctgccg ctattacgcc aaactggcgc gcgagcagtt cggcgcgccg
     4561 ataacgctga ccgggccggt gggcgagcgc aacacctatg gcctgcatgg gcgcggcgtc
     4621 ttcgtctgca tcagcccgtg gaacttcccg ctggccatct tcaccggtca ga
//
LOCUS       DASVLL010000103         6314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_328027,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000103 DASVLL010000000
VERSION     DASVLL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328027"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VF402_01705"
     CDS             complement(<1..438)
                     /locus_tag="VF402_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7799033.1"
                     /translation="MTNKRVRKAVIPVAGLGTRVLPGTKTVPKELLNVVDRPILSYIV
                     EEARAAGIEHIVFVTGRAKGAIEDYFDHQVELEAQLAAKGKTEALDEILAELPRPGAM
                     SYVRQMQPHGLGHAVWCARDIVGDEPFAVILPDMLMDAPVPALK"
     gene            769..1989
                     /locus_tag="VF402_01710"
     CDS             769..1989
                     /locus_tag="VF402_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEX7799034.1"
                     /translation="MPMQNSLKAAAAAMALSVLTLGCAAAAQAEEHGAYTLEKPKPPV
                     AMTPPMGWNAWNHFGCDINEAKIRGAADAMAASGMKDAGYRYVIIDDCWQVGRDAAGN
                     IVADPAKFPSGIKALADYIHARGLKFGIYSDAGLKTCGGRPGSRGHEFQDAKQYAAWG
                     VDYLKYDWCNTGSDDAQADYRIMAMALRESGRDIVFSLCEWGNHKPWEWAGEVGHLWR
                     TTGDIWDSWDRNKDYQHGVTDILDQQVGLEKYAGPNRWNDPDMLEVGNGGMSDTEYRA
                     HFSLWAMLAAPLIAGNDLSNMSAATKAILLNKDVIAVDQDALGQEGRRVSKDGDFEVW
                     ARPLAGGDTAVILLNRSKAAHVMTADWAALGLPAKADVKDLWSKAVKKDVATGYTADV
                     PPHGVVMLRVTPKG"
     gene            complement(1991..2695)
                     /locus_tag="VF402_01715"
     CDS             complement(1991..2695)
                     /locus_tag="VF402_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7799035.1"
                     /translation="MRSEFGVSLAYGLLRTLGQSIVTGEFEKTGFPTEAELCQKFGAS
                     RTVMREAVKMLSAKGLLSSRQRQGTRVEPVENWNLLDPDVLRWLMERPYSNKIFKEFT
                     EMRLAIEPTAAALAAQVQNRLAIAEIREGLEAMGAAKDPDASLQADIDFHVAILKASG
                     NPFFWRLKPMITNALNLSIQLTNKIAGHTADLAQHEAILIAIEKGDATAAEKASEAIL
                     RESLTFIEKTAEAPAA"
     gene            2899..4554
                     /locus_tag="VF402_01720"
     CDS             2899..4554
                     /locus_tag="VF402_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HEX7799036.1"
                     /translation="MKLIGALVASLAAASLLAGTAFAGDMPQLVSRNGKHALMVDGAP
                     YLLLGGQANNSSNYPAVLPKVWPAIADMKANTLVMPVAWEQIEPQEGRFDFAFVDTLV
                     AQAREHKVRLVILWFGTWKNTGPSYTPDWVKTDNKRFPRLLNKDGSTSYALSPLYDST
                     READKKAYVALLTHIKAIDGDRHTVIMMQVENETGVYGAARDYSKKADALMRQPVPAA
                     LIKAMGRRALKASGDWREVFGADADEYFHAWSIARYCDDIAAAGKAVYPLPAYMNDAL
                     KDPLNPAQAAGSYASGGPSYNVLDIYKVAAPHIDILAPDLYTPESKSYEATLDLYARP
                     DNALFIAESGNKPIYARYLFSALGHAAIGFDPFGFDYTGYSNYPLGSQATDKSMVAPF
                     AELYGVFAPMARVWAGLSFNSQVWGVSEPDDHSAQDLDLGPLWSARVTYRQWQFGLPE
                     WDPQNKNGYPEGSDVPSGGVAVARLADNQFLVVGHNARITFGAGPKTKDKGFLMSRVE
                     EGHYDADGQWVFERVWNGDQTDYGLNFVGAPRVLKVTLATYTK"
     gene            4771..6012
                     /locus_tag="VF402_01725"
     CDS             4771..6012
                     /locus_tag="VF402_01725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7799037.1"
                     /translation="MTTPNPANPVTTTDLKALPFTARKGVQLLQKVWEIGALEIIFPD
                     GKSLTLNGRRPGPAGVLLVKDFHFMQRVLASGDIGFCDGFREGEWDTPDLATLLEVLT
                     LNSEKLGQLFTGNIVTRTVNNVLHALRRNTRTGSRRNIFEHYDLGNSFYDQWLDQTMT
                     YSSALFDGERFREHKDLSAAQTAKYARLAELVDLQPGQHVLEIGCGWGGFAQYAAETV
                     GANVTGVTISRAQHDYAVERMARLGLSDKVRIRLMDYRDIQGQFDAVVSIEMFEAVGE
                     SYWGVYFGKLREVLKQGGKAGLQIITIADEMFEDYKSRADFIQKYVFPGGMLPSAQKL
                     VEQTHAAGLKLVSDFGFGHDYAETLKQWGQRFEAAWKEGRIKGFDAQFRKLWLFYLAY
                     CEAGFRTERTNVLQLQLQRAD"
BASE COUNT         1203 a         1941 c         2057 g         1113 t
ORIGIN      
        1 tttcagcgcc ggaaccggcg cgtccatcag catgtcgggc aggatgaccg cgaacggctc
       61 gtcgccgacg atgtcgcgcg cgcaccagac cgcgtggccg aggccgtgcg gctgcatctg
      121 gcgcacatag ctcatcgcgc cgggtctggg cagttcggcc aggatctcgt ccagcgcctc
      181 cgtcttgccc ttggcggcca gttgcgcctc cagctcgacc tgatggtcga aatagtcctc
      241 gatcgcgccc ttggcgcggc cggtgacgaa gacgatatgc tcgatccccg ccgcgcgcgc
      301 ctcctcgacg atgtaggaca ggatgggccg gtcaacgacg ttgagcaact ccttggggac
      361 ggttttggtg ccgggcagga cacgggtgcc gagccccgcc acgggtatga ccgccttgcg
      421 gacacgctta ttcgtcaaga tgacctcttt gcagcaatgg gcgcggccgc ttcctcgccg
      481 ccgcgcgtct tctggacgcc aaggttagag aggcggggcg gaaaaaacaa ccgcatcgcg
      541 gcgaattctt cccggaagcg aaaaaaagcc cccgaacagg gagccgttcg ggggaccatg
      601 tcagacatct gtgcgcaggg gcgcacaggg agggaataca tctgcttcgg ataatatagc
      661 gcgatttacg attaaagtaa attgttttat ttaattggac ttggcggcgc gccctaaaat
      721 tgtaagacta atgcgcagcc agagagacga aatccaggga gacacaccat gcctatgcaa
      781 aacagcctga aagccgccgc cgccgcgatg gccctgtcgg ttctgaccct gggctgcgcc
      841 gctgcggcgc aggccgaaga gcacggcgcc tacacgctgg aaaagcccaa gccgccggtg
      901 gcgatgacgc cgccgatggg ctggaatgcg tggaatcatt tcggctgcga tatcaatgaa
      961 gccaagatca ggggcgcggc ggacgcgatg gcggcgtcgg gcatgaagga tgcgggttat
     1021 cgctatgtga tcatcgacga ttgctggcag gtcgggcgcg acgccgccgg gaatatcgtg
     1081 gccgatccgg cgaagtttcc gtccgggatc aaggccctgg ccgactatat ccacgccagg
     1141 ggcctgaagt tcggcatcta ttccgacgcg gggctgaaga cctgcggcgg gcggcccggt
     1201 tcgcgcggcc atgagtttca ggacgcgaag cagtacgcgg cctggggcgt cgactacctg
     1261 aaatacgact ggtgcaatac ggggtccgac gacgcgcagg ccgattaccg catcatggcg
     1321 atggcgctgc gcgagtcggg gcgcgacatc gtgttttcgc tgtgcgaatg gggcaatcac
     1381 aagccgtggg aatgggcggg cgaggtcggc catctgtggc gcaccaccgg cgacatctgg
     1441 gatagctggg accggaacaa ggattaccag cacggcgtca ccgacattct cgatcagcag
     1501 gtcgggctgg aaaagtacgc cgggccgaac cgctggaacg acccggacat gctcgaagtc
     1561 ggcaacggcg gcatgagcga taccgaatac cgcgcgcact tctcgttgtg ggccatgctg
     1621 gccgcgccgc tgatcgcggg caacgacctg tcgaacatga gcgccgcgac caaggcgatc
     1681 ctgctcaaca aggacgtgat cgccgtcgat caggacgcgc tgggccagga gggccgtcgc
     1741 gtgtccaagg acggcgattt cgaggtctgg gcgcgtccgc tggcgggcgg cgacacggcg
     1801 gtgatcctgc tcaaccgctc gaaggcggcg cacgtcatga cggcggactg ggccgcgctg
     1861 ggcctgcccg cgaaggccga cgtgaaggat ctgtggtcga aggcggtcaa aaaggacgtc
     1921 gcaaccggct ataccgccga cgttccgccg catggcgtcg tgatgctgcg cgtcacgccg
     1981 aaggggtagg ctaggccgcc ggcgcctcgg cggtcttttc gatgaaggtc agtgattcgc
     2041 gcaaaatcgc ttcggacgcc ttttcggcgg cggtggcgtc gcctttttcg atggcgatca
     2101 ggatggcctc gtgctgggcc aggtcggcgg tgtggccggc gatcttgttg gtgagctgga
     2161 tcgacaggtt gagcgcgttg gtgatcattg gcttgagccg ccagaagaac ggattgcccg
     2221 aggctttcag gatggcgacg tgaaaatcga tatccgcctg caaggaggcg tcggggtctt
     2281 tggcggcgcc catcgcctca agcccttcgc ggatttcggc gatggccagc cggttctgca
     2341 cctgggcggc cagcgcggcg gcggtcggct cgatggccag ccgcatctcg gtgaattcct
     2401 tgaaaatctt gttggagtag gggcgctcca tcagccagcg cagcacgtcg gggtccagaa
     2461 ggttccagtt ctcgaccggc tcgacgcgcg tgccctgacg ctggcgcgac gacagcagcc
     2521 ccttggccga cagcatcttg accgcctcgc gcatcacggt gcggctggcg ccgaatttct
     2581 gacacagctc cgcctcggtc ggaaagcctg tcttttcaaa ctctccggtg acgatggact
     2641 ggcccagcgt gcgcaaaagc ccgtaggcca gactgacgcc aaattccgaa cgcatgacgg
     2701 tttcccctca ttaaatacac gacaatcatc gaatcgtcgg cgccgccgac cgatccctga
     2761 gcggagatta cacaaaaagt tcagcgcgcg acataattca tggctggact tgcggcgctg
     2821 ccggagtttt tatatgacaa ttttacaagt aataagccgc atcacctgtg aagcggcgcc
     2881 gggttgggga gaaacgcaat gaaattgatc ggcgctctgg ttgcgtccct ggctgccgcc
     2941 tcgcttctgg ccgggacggc gttcgcgggc gacatgccgc aactggtcag ccggaacggc
     3001 aagcacgccc tgatggtcga cggcgcgccc tatctgctgc tgggcggaca ggcgaacaat
     3061 tcgtccaact atccggcggt cctgcccaag gtctggcccg ccatcgcgga catgaaggcc
     3121 aacaccctgg tcatgccggt ggcgtgggag cagatcgagc cgcaggaggg ccggttcgat
     3181 ttcgccttcg tcgacacgct ggtcgcgcag gcgcgcgagc acaaggtccg cctcgtcatc
     3241 ctgtggttcg ggacgtggaa gaacaccggc cccagctata cgccggactg ggtcaagacc
     3301 gacaacaaac gcttcccgcg cctgctcaac aaggacggct cgacctccta cgccctgtcg
     3361 ccgctgtacg attcgacgcg cgaggcggac aaaaaagcct atgtcgcctt gttgacgcac
     3421 atcaaggcca tcgacggcga ccggcacacg gtcatcatga tgcaggtcga gaacgaaacc
     3481 ggcgtctatg gcgcggcgcg cgactattcc aaaaaggccg acgcgctgat gcgccagccc
     3541 gtgcccgcag cgctgatcaa ggcgatgggc aggcgcgcgc tgaaggcgtc gggcgactgg
     3601 cgcgaggtgt tcggcgccga cgccgacgaa tatttccacg cctggtcgat cgcgcgctat
     3661 tgcgacgaca tcgccgcggc gggcaaggcg gtctatccgt tgccggccta tatgaacgac
     3721 gcgctgaaag acccgctcaa tccggcgcag gcggcgggtt cctacgcttc gggcggcccc
     3781 agctataacg tgctggacat ttacaaggtc gccgcgccgc acatcgatat cctggcgccc
     3841 gacctctaca cgccggaatc gaaatcctac gaggcgacgc tcgatctcta tgcgcggccg
     3901 gacaatgcct tgttcatcgc cgaaagcggc aataagccga tctatgcgcg ctatctgttc
     3961 agcgcgctgg gccacgcggc catcggcttc gatccgttcg gcttcgacta taccggctat
     4021 tccaactacc cgctgggcag tcaggcgacc gacaagagca tggtcgcgcc gttcgccgag
     4081 ctttacggcg tcttcgcccc gatggcgcgc gtctgggccg ggctgagctt caacagtcag
     4141 gtctggggcg tctccgaacc cgacgaccac agcgcgcagg atctcgatct cggcccgctg
     4201 tggtcggcca gggtgaccta tcgccagtgg cagttcggcc tgccggaatg ggatccgcag
     4261 aacaagaacg gctatcccga aggctcggac gttccgtccg gcggcgtcgc cgtggccagg
     4321 ctggccgaca accagttcct cgtcgtcggc cataacgccc gcatcacctt cggcgccggg
     4381 ccgaagacca aggataaggg cttcctgatg tcgcgcgtcg aggaaggcca ttacgacgcg
     4441 gacggtcaat gggtgttcga gcgcgtctgg aacggcgacc agaccgatta cggcctgaac
     4501 ttcgtcggcg cgcccagggt gctgaaagtc acgctggcga cctatacgaa ataaggccgg
     4561 gcgcgaaaca aggtcgggcg cctttcaatt cccccagcgc ggcgagggcg atcgggggag
     4621 gcgccgtccg tcggccgaat gcgagcgtcc gtcggccgca cccccttgcc aacggtaaaa
     4681 tccggtccat attggaagga agcgggccgc aatccgcagg cccgaagttc gcgtatccat
     4741 gcgtgtcact cttctgcatg aggccgcccg atgacgaccc cgaaccccgc caatccggtg
     4801 acgacgaccg acctgaaagc cctgccgttc acggcgcgca agggggttca gctcttgcag
     4861 aaggtgtggg agatcggcgc gctggagatc atctttcccg acggcaagag cctgaccctg
     4921 aacggtcggc ggccgggacc ggcgggcgtg ctgctggtca aggatttcca cttcatgcag
     4981 cgcgtgctgg cctccggcga tatcggcttt tgcgacggct tccgcgaagg cgaatgggat
     5041 acgccggatc tggcgaccct gctggaggtg ctgacgctca attcggaaaa gctggggcag
     5101 ttgttcaccg gcaatatcgt cacgcggacg gtcaacaacg tgctgcacgc cctgcgccgg
     5161 aacaccagga ccggctcccg gcgcaatatt ttcgagcatt acgatctcgg caacagcttc
     5221 tacgatcagt ggctggacca gaccatgacc tattcgtcgg ccctgttcga cggcgagcgc
     5281 ttccgcgaac acaaggatct gagcgcggcc cagacggcga aatacgcgcg tctggccgag
     5341 ctggtcgatc tgcagccggg ccagcatgtg ctggagatcg gctgcggctg gggtggcttt
     5401 gcgcaatatg cggccgagac ggtcggcgcg aacgtgaccg gcgtgaccat ctcgcgcgcc
     5461 cagcacgatt atgcggtcga gcgcatggcg cggctgggcc tcagcgacaa ggtgcgcatc
     5521 cggctgatgg attatcgcga cattcagggc cagttcgacg cggtggtgtc gatcgaaatg
     5581 ttcgaggcgg tcggcgaatc ctactggggc gtctatttcg gcaagctgcg cgaggtgctg
     5641 aaacagggcg gcaaggcggg tttgcagatc atcaccatcg ccgacgagat gttcgaggac
     5701 tataaatcgc gcgccgactt catccagaaa tacgtcttcc ccggcggcat gctgccctcg
     5761 gcgcagaagc tggtcgagca gacccatgcg gcggggctga aactggtgtc ggacttcggc
     5821 ttcggccacg actacgccga gacgctgaaa cagtggggcc agcgcttcga ggcggcgtgg
     5881 aaggaaggcc gcatcaaggg cttcgacgcc cagttccgca agctgtggct gttctacctc
     5941 gcctattgcg aagccggctt ccgcaccgaa cgcaccaatg tcctgcaact gcaattgcag
     6001 cgcgcggact gaggttacgc gcgaggcgac tctccgccaa ggttgcatgc ccggcgttca
     6061 ggccatccga atcccgtctc tctgatttcc gagcggcgtc aaaataccgc aattaaatct
     6121 ttcttcactt ctgataaaca ataaccatga aaatagtgcg gccttttgaa ggggccgccc
     6181 gtttcagggc gcgtaccagc tcgtatttcc caaatgatga tcgacgccgt ccattccggg
     6241 cgcggatttt gtcatgcctg caaaacaata atcaagccac agcccggtaa ggtgaaaaag
     6301 gatgttgtcc cgct
//
LOCUS       DASVLL010000104         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_113236,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000104 DASVLL010000000
VERSION     DASVLL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113236"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF402_01730"
     CDS             complement(<1..268)
                     /locus_tag="VF402_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family peptidase"
                     /protein_id="HEX7799038.1"
                     /translation="MNRSRRFYLGAAAFAALAAGAPRAVLAAAPDMADNPFAQPSALP
                     LQAPPFDRIKDTDYQPAIEEGMRQQLAEIDAIANDPAPPTFENTL"
     gene            433..1959
                     /locus_tag="VF402_01735"
     CDS             433..1959
                     /locus_tag="VF402_01735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799039.1"
                     /translation="MRQSAPTQPAARLDPKDVRSIIFGLMTVLLLAALDQTIVSTALP
                     TIGRALGDAQHLPWMVTAYLLASTVVTPLYGKLADVIGRRTTLLTAIGIFLLGSLLCS
                     LSPNMLALIGARALQGLGGGGLMSLVQTVISDVVTPQERGRIQGVFAAVFTSASLGGP
                     ILGGALSEHLGWASIFWVNLPVGAVALWVVSRGLMKLPRYDRPHRIDWLGAGLMTAAA
                     LILMLAINTRGAGLMGVPVWAAYVVSGVFWLLFGLRLRFAAEPLIPTGILKDGVIVRA
                     ILVATLSMGALMSVGAYNPIYIQLIYHLGPTAAGMVMTPLALGVVGGSILSGQMMARS
                     KGKGYKILPTAGLLVGAVMYAALAVIGDRLSLWAYIGALVVANAAVGSTFPVSTVVVQ
                     NSVERHQMGTATGVMNFFRSLGGAIMVAVFGAVLFGEIYALLGDSFNGALSADTMSHV
                     ADPARIYKPVFIGAAAAMGAAFLCLLTMEARPLRSRDMPAAKAAEEAAGETLSGADFH
                     "
BASE COUNT          287 a          687 c          749 g          383 t
ORIGIN      
        1 ccagcgtgtt ctcgaaggtc ggcggcgccg gatcgttggc gatggcgtcg atttcggcca
       61 gttgctgtct catgccctcc tcgatggcgg gctggtaatc cgtgtccttg atcctgtcga
      121 acggcggcgc ctgcaagggc agggcgctcg gctgggcgaa cgggttatcc gccatgtcgg
      181 gcgcggcggc gagtacggcg cgcggggcgc ccgccgccag cgcggcgaaa gcggcggcgc
      241 ccagataaaa acggcgactg cggttcatga cgacctcctc aaggactgct gataaagtca
      301 ttgcatatga aaccggctga aaggaagata aaaaatggcg ggcgggcgga cgcgccgctc
      361 acaaacacgt tgccaaggcc gccgccttcg cgcaccctgc gcggccccgc caatggatcg
      421 aagagacgga tcatgcgcca gagcgccccg acccagcccg ccgcgcgcct cgatccgaag
      481 gacgtgcgct cgatcatttt cggcctgatg accgtgcttt tgctggcggc gctggaccag
      541 accatcgttt cgaccgccct gccgacgatc gggcgggcct tgggcgacgc gcagcatctg
      601 ccgtggatgg tcaccgccta tctgctggcc tcgaccgtgg tgacgccgct ctacggcaag
      661 ctggccgacg tgatcgggcg gcgcacgacc ctgttgacgg ccatcgggat ttttctgctc
      721 ggctcgctct tgtgcagcct gtcgccgaac atgctggccc tgatcggcgc gcgcgccctg
      781 caagggctgg gcggcggcgg gctgatgtcg ctggtgcaga cggtgatttc cgacgtggtc
      841 acgccgcagg agcgcgggcg gattcagggc gtgttcgccg ccgtcttcac ctcggccagc
      901 ctcggcggcc cgatcctggg cggcgccctg tccgagcatc tgggctgggc gtcgatcttc
      961 tgggtcaacc tgccggtcgg cgccgtcgcc ctgtgggtgg tgtcgcgcgg cctgatgaag
     1021 ctgccgcgct acgaccgtcc gcaccgcatc gactggctgg gggcggggct gatgacggcg
     1081 gcggcgctga tcctgatgct ggccatcaat acgcgcggcg ccgggcttat gggcgtgccg
     1141 gtctgggcgg cctatgtcgt gtccggggtc ttctggctgt tgttcggcct gcgcctgcgt
     1201 ttcgccgccg agccgctgat cccgaccggg attctcaagg acggggtgat cgtgcgcgcg
     1261 attctggtcg ccactttgag catgggcgcg ctgatgtcgg tcggcgctta taatccgatc
     1321 tatatccagc tcatctatca tctcggcccg acggcggcgg gcatggtcat gacgccgctg
     1381 gcgctgggcg tggtcggcgg ctcgatcctg tcggggcaga tgatggcgcg cagcaagggc
     1441 aagggctaca agatcctgcc gaccgccggc ctgctggtcg gggccgtgat gtacgccgcg
     1501 ctggccgtga tcggcgaccg tctgtcgctg tgggcctata tcggcgcgct ggtcgtggcc
     1561 aatgcggcgg tcggctcgac cttccccgtc tcgaccgtgg tggtgcagaa ttcggtcgag
     1621 cggcatcaga tgggcacggc gaccggggtg atgaacttct ttcgcagcct gggcggcgcg
     1681 atcatggtgg cggtgttcgg cgccgtcctg ttcggcgaaa tctacgcgct gctgggcgac
     1741 agcttcaacg gcgcgctttc cgccgatacg atgagccatg tcgccgatcc ggcgcgcatc
     1801 tataagccgg tgttcatcgg cgccgccgcc gccatgggcg cggccttcct gtgcctgctg
     1861 acgatggagg cgcgccccct gcgttcgcgc gacatgccgg cggccaaggc ggcggaggaa
     1921 gccgccggcg agactctcag cggcgcggat tttcattgag aaacctcccc tctccgcgat
     1981 ggcggggaga ggacgaggcg caaggcgaag ccgccgatcc tcgggtgagg ggccgcccgt
     2041 cggccgagcg caaccggcaa acagcccctc acccgatcgc catcgctttc gctcggctct
     2101 cgtcct
//
LOCUS       DASVLL010000105         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_167622,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000105 DASVLL010000000
VERSION     DASVLL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167622"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VF402_01740"
     CDS             complement(<1..825)
                     /locus_tag="VF402_01740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7799040.1"
                     /translation="MKFSLSWLKDYLDTEADIDAVAAAMTRAGLEVEDIHDPAKLLKP
                     FSVARVVEAARHPNADKLQVLQVDTVDGRKEIVCGAPNARAGMTTIYAPIGAYVPGLD
                     VTLVEKPVRGVVSNGMMCSAAELQLSDESDGILDLSGDLPVGAPATEVFGLEPVIDFE
                     VTPNRPDWLGVNGIARDLAATGLGAFRDKTIPPVAGQFPCPISVSVDGEACRHFTGRV
                     IRGVKNGPSPAWLQQKLTAIGLKPISALVDVTNYLSYDRARPLHVYDVAKLTGTHLM"
     gene            complement(880..1953)
                     /gene="pheS"
                     /locus_tag="VF402_01745"
     CDS             complement(880..1953)
                     /gene="pheS"
                     /locus_tag="VF402_01745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7799041.1"
                     /translation="MTAYTDLQHDIEAEIAAASDLAALDAVRVSALGKTGRISGLLKT
                     LGALPPEARKTTGAAINAVRDAVTAALEARKAALEAAHLAQRLENERVDLSLPSARRP
                     RGGVHPTMQVMDEIVAIFAEMGFAVAEGPDIEDDFHNFTALNFPDKHPAREMHDTFFF
                     NPDENGVRKLLRTHTSPVQIRTMLSGKPPFRLIAPGRVFRCDSDQTHTPMFHQVEGLV
                     IDKGAHMGQLKWVLETFISRFFETDSVTTQFRPHHFPFTEPSAEMDVRCSWEKGELKV
                     GTGESWLEILGCGMVHPNVLKACGVDPEEYQGFAWGMGVDRLAMLKYGVPDLRDMFAA
                     DTRWLAHYGFSGFVAPNPASGLS"
     gene            complement(2148..2507)
                     /gene="rplT"
                     /locus_tag="VF402_01750"
     CDS             complement(2148..2507)
                     /gene="rplT"
                     /locus_tag="VF402_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7799042.1"
                     /translation="MARVKRGVTSHAKHKKVLKLAKGFYGRRKNTIRTAKAAVDKAGQ
                     YAYRDRRNKKRNFRSLWIQRINAAARLEGFTYSQFIHGLTVAGIDMDRKVLSDVAMND
                     EAGFKAIADKVRTALAA"
     gene            complement(2524..2721)
                     /gene="rpmI"
                     /locus_tag="VF402_01755"
     CDS             complement(2524..2721)
                     /gene="rpmI"
                     /locus_tag="VF402_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7799043.1"
                     /translation="MPKLKTKSGAKKRFRFTASGKVKAGVAGKRHRLISHNAKYIRQN
                     RGTEVMSDADIVKIKSYMPYA"
     gene            2904..3371
                     /locus_tag="VF402_01760"
     CDS             2904..3371
                     /locus_tag="VF402_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7799044.1"
                     /translation="MKLTLPVFPIHGGCQCGAVRYTLKAAPVVFYLCHCADCQKQSSS
                     AFGESVKARRGDVEISGETARFETTADSGRPKLCEFCPRCGTRMFHGRRPDAETFNIK
                     GGTLDDARWLVPAGHIWTASKQALFVIGEDELSYPRSPDYDALTRRWREMCGV"
     gene            3421..>3806
                     /locus_tag="VF402_01765"
     CDS             3421..>3806
                     /locus_tag="VF402_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEX7799045.1"
                     /translation="MRSVRLLVLPLLLLLTAPLAGCLSIPKILYDTAQTEALSVRLKD
                     GYEQRYIRYMPAHAAADKRLPLIVFLHGSGEAGDDPYAVLANGPWHYAAAHPDFPFII
                     LAPQEDHDDEWSPRRLDEWLIQAEKGL"
BASE COUNT          632 a         1178 c         1287 g          709 t
ORIGIN      
        1 catcagatgc gtcccggtca gcttggccac gtcatagacg tgcagcgggc gggcgcggtc
       61 gtaagacagg tagttggtga cgtccaccag cgcgctgatc ggcttgaggc cgatggcggt
      121 cagcttttgt tgcagccatg cgggcgacgg gccgttcttg acgccgcgaa tgacgcgacc
      181 ggtgaagtgg cggcaagcct cgccgtcgac gctgacgctg atcgggcagg ggaactgacc
      241 cgcaaccggc gggatcgtct tgtccctgaa cgcgcccagc cctgtggcgg ccagatcgcg
      301 cgcgatgccg ttgacgccca gccagtcggg ccggttcggc gtgacctcga agtcgatcac
      361 cggttcgagg ccgaacacct ccgtggcggg cgcgccgacc ggcagatcgc cgctcaggtc
      421 gagaatgccg tcgctctcgt cggacagttg cagttcggcg gccgagcaca tcatgccgtt
      481 cgacaccacg ccgcgcaccg gcttttccac cagcgtgacg tcgaggccgg gcacgtaggc
      541 gccgatgggg gcgtagatgg tggtcatgcc cgcgcgcgcg ttcggggcgc cgcagacgat
      601 ctccttgcgc ccgtcgacgg tgtcgacctg caagacctgc aatttgtcgg cgttcgggtg
      661 tctggcggcc tcgaccaccc tggcgaccga aaagggtttc agcagctttg ccggatcgtg
      721 aatgtcctcg acctccagcc ccgcgcgcgt catggcggcg gcgaccgcat cgatatcggc
      781 ctcggtgtcg agatagtctt tcagccagga gagggagaat ttcatcggat atttcttatg
      841 ttctggggtc aagggggcga tgcccccttg tcttaattct tacgacaggc cgctggccgg
      901 gtttggcgcg acgaagcctg agaagccgta gtgcgccagc cagcgcgtgt cggcggcgaa
      961 catgtcgcgc aggtcgggca cgccgtattt gagcatggcg aggcgatcga cgcccatgcc
     1021 ccaggcgaag ccctgatatt cctccgggtc gacgccgcaa gccttcagca cgttcggatg
     1081 caccatgccg cagccgagga tttccagcca cgactcgccg gtcccgacct tcagctcgcc
     1141 tttttcccac gaacagcgca cgtccatttc ggcggacggc tcggtgaacg ggaagtggtg
     1201 cgggcggaac tgggtcgtca cgctgtcggt ctcgaagaag cgcgagatga aggtttccag
     1261 cacccatttg agctggccca tatgcgcgcc cttgtcgatg acgaggccct cgacctgatg
     1321 gaacatcggc gtgtgggtct ggtcggaatc gcagcggaac acccggcccg gcgcgatcag
     1381 gcggaagggc ggcttgcccg acagcatggt gcggatctgc accggcgagg tatgggtgcg
     1441 cagaagcttg cgcacgccgt tttcatcggg gttgaagaaa aacgtgtcgt gcatttcgcg
     1501 cgccgggtgc ttgtccggga agttgagcgc ggtgaagtta tggaagtcgt cctcgatgtc
     1561 cgggccttcc gccaccgcga agcccatctc ggcgaagatg gcgacgatct cgtccatgac
     1621 ctgcatggtc ggatgcacgc cgccgcgcgg acggcgcgcg ctgggcaggg agaggtcgac
     1681 gcgctcgttt tccagccgct gcgccaggtg cgcggcctcc agcgcggcct tgcgcgcctc
     1741 cagcgcggcg gtcaccgcgt cgcgcacggc gttgatcgcc gcgccggtgg tcttgcgcgc
     1801 ctccggcggc agggcgccga gggttttcaa aagcccggaa atgcgcccgg tcttgcccag
     1861 agccgacacg cgcacggcgt ccagcgcggc caggtccgaa gcggcggcga tttcggcttc
     1921 gatgtcgtgt tgcaggtcgg tataggcggt catggttttg tcgtttgcat aagggatgcg
     1981 tgtgtttagg cgaaagcggt agcggacgca attcggcagg gaaagaaacc ccgccgccac
     2041 atctcagtcg ctgcgcggct tcgtgcggta tgagatgagc gtgaaaagaa aaaccccgcg
     2101 accgtcgccg gcgcggggtt cttcaaaagc gttcaagccg taaggcctta agccgccaga
     2161 gccgtgcgca ccttgtccgc gatggccttg aagccggctt cgtcgttcat cgcgacgtcg
     2221 gacagaacct tgcggtccat gtcgatgccc gcgacggtga ggccgtggat gaattgcgag
     2281 taggtgaagc cttccagacg cgcagccgcg ttgatgcgct gaatccacag gctgcggaag
     2341 ttgcgcttct tgttgcggcg gtcgcgatag gcgtattggc cggccttgtc gacggccgcc
     2401 ttcgcggtgc ggatggtgtt cttgcggcgg ccatagaaac ccttggcgag tttcaggacc
     2461 ttcttgtgtt tggcgtggga cgtgacgcct cttttaacgc gagccatgtt ttactgctcc
     2521 tctttaggcg tacggcatgt aggacttgat cttcacaatg tcggcgtccg acataacctc
     2581 ggtgccgcgg ttctgacgga tgtacttggc gttgtgagag atcaggcggt gacgcttgcc
     2641 ggcgacgccg gccttcacct tgccagaggc agtaaagcgg aagcgcttct tggcgcccga
     2701 cttggtcttc agcttgggca tttttcatac ggccggatta gggccgccct cttgggatta
     2761 ggatgaaccc ccgccaaggc aatgccgtat tggcccggcg gggatggatt tcgagcgccc
     2821 ggcccgaagg cggcgcgcag gaaacgcggc agatacagga aaagggctga cgaagcaagc
     2881 cttgagtcgt tataaaagac cgcatgaagc tgaccctgcc cgtattcccc atccacggcg
     2941 gctgccagtg cggcgccgtg cgttatacgc tcaaagccgc tccggtggtc ttttatctgt
     3001 gtcactgcgc cgattgccag aaacagtcgt cctcggcctt cggcgagtcg gtcaaggcgc
     3061 ggcgcggcga tgtggagatt tcgggcgaga cggcgcgctt tgaaaccacg gcggacagcg
     3121 gcaggcccaa gctgtgcgaa ttttgcccca gatgcgggac gcggatgttc cacgggcggc
     3181 ggccggacgc cgaaaccttc aatatcaagg gcggcacgct ggacgacgcc cgctggctgg
     3241 tcccggcggg tcatatctgg accgcgtcga aacaggccct tttcgtcatc ggcgaggacg
     3301 agctgagcta tccgcgcagc cccgactacg acgccctgac gcggcggtgg cgcgagatgt
     3361 gcggggtatg agctgaccat ccggcttgcc aagcggcggc gtccggcgca tcttcgcgcc
     3421 atgaggtccg tccgtctgct cgtcctgccg ctgctgctgc tgctcaccgc cccgctcgca
     3481 ggctgcctgt cgatcccgaa aatcctttac gacacggcgc agaccgaggc cctgtcggta
     3541 aggctgaagg acggctacga gcagcgctat atccgctaca tgcccgccca cgccgcggcg
     3601 gacaaacgcc tgccgctgat cgtgttcctg cacggttcgg gcgaggcggg cgacgatccc
     3661 tacgccgtgc tggccaacgg gccgtggcat tacgcggccg cgcaccccga ttttccgttc
     3721 atcatcctgg cgccgcagga ggaccacgac gacgaatggt cgcccaggcg cctcgacgaa
     3781 tggctgatac aggcggaaaa gggcct
//
LOCUS       DASVLL010000106         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_113315,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000106 DASVLL010000000
VERSION     DASVLL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113315"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VF402_01770"
     CDS             <1..1680
                     /locus_tag="VF402_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX7799046.1"
                     /translation="HFTAAINGQTKFQSNVGERVSFFVVVGDTPEQIYSAYARVTGKT
                     PIPPKSAFGLIQSKARYDSQAEVLRVANTYRQKGYPLDIMVIDWFYWTRMGQMDIDPA
                     QFPDPDAMNKQLHDMGMQSIISIWPRFETSGRYYNELAAKGYFLKDKNGNPVDGLPFR
                     SDRTGALIDATNPDARKWFWDHARDNILSHGFDYPWLDETEPDLVPDGSFFSIGSGDR
                     YHNVFPLLHVEGVADGMRQWKPDKRVLILSRAAYLGSQRTGALFWSSDINPSWEALRR
                     QIPTGLNMTASGIAYWGNDIGGWQWLPQTTTATKPPLLDPSDARDVVGQNNDYPELLT
                     RWFEYGTFLPTLRLHGDRKHTEIWAFGHEAEAIMARYDKLRYELIPYIYSSAKHTYDT
                     GAPFMRALWMDFPNDPNVSDIGTEYMFGPAFLVAPVTEQGQTEKSVYLPAGSDWYNYW
                     TNQKLTGGQWVKTAAPIDQIPVFVKAGSIVPVGSDIPSTATKQTITALKVYPGANGGF
                     SLYDDDGHTYDYETGKGVTASQLTWDDATGKLSATGALAKSAAGLVQVIGK"
     gene            1867..2631
                     /locus_tag="VF402_01775"
     CDS             1867..2631
                     /locus_tag="VF402_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7799047.1"
                     /translation="MTLDPDKRKRLYRRVAEEIIRDVRGGVYKVGAKLPAERDLADSM
                     DASRTTIREAIIALEMLGVVEVRHGAGVFVTGEGIAATSAYATQDLNVGAFELIEARM
                     VIESETAAIAARVATDKDVEQLAELVRVMETGQPAAIEQADREFHLLIARITENGALI
                     DTVRNLWNLRAASPLASAIISRAHDSGLEARTREHRPILDAIRARDPAAARNAMREHM
                     QCVRDYVLEATEMAEIESLRNQLRSQRDAWASRTAP"
     gene            3557..>4637
                     /locus_tag="VF402_01780"
     CDS             3557..>4637
                     /locus_tag="VF402_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799048.1"
                     /translation="MNDLSRTQIMRRRLTGGTALALVALFAGASLAHAGLAHAQTAAR
                     NSQSTAKSGNDETTLVIVTGTRASQQSAIDRKKRAKTATDSIAAEDIGTFPDNNIDEA
                     ISRIAGIQLDRGDFGEGQSVTVRGQSAENNRVELDGVGVMNTSGGLVSGAGDARGADL
                     REIPADIVKSLDVVKGTTAAMTEGSLGGSIHINTRTGLDFKKPYFQFRASESSNTLDK
                     KWTPSWSAIAARKFLNGRLGVIANVNYSEISNSADSEQPNTSGTAGYYRNVDLDQSPE
                     KTFTYNPAVSEMVGDTKLSNSDNTMLDVIKKSAAAQTPQDCLAAFPVPAWTPTTKAPG
                     PSSKAAEAGYELQSCLNQWNDYTPSL"
BASE COUNT          975 a         1592 c         1346 g          724 t
ORIGIN      
        1 catttcaccg ccgccatcaa cggccagacg aagttccagt ccaatgtcgg cgagcgcgtc
       61 tccttcttcg tcgtggtcgg cgacacgccg gagcagatct attccgccta tgcgcgcgtc
      121 accggcaaga cgccgatccc gcccaaatcc gccttcggcc tgatccagtc caaggcgcgc
      181 tacgattcgc aggccgaggt gctgcgcgtc gccaacacct accgtcagaa gggctatccg
      241 ctcgacatca tggtgatcga ctggttctac tggacccgga tgggccagat ggacatcgac
      301 ccggcgcagt tccccgatcc cgacgcgatg aacaagcaac tgcacgacat gggcatgcag
      361 tcgatcatct cgatctggcc gcgcttcgag acgtccgggc gctattataa cgagctggcc
      421 gccaagggct atttcctcaa ggacaagaac ggcaaccccg tcgacggcct gcccttccgc
      481 tccgaccgca ccggcgcgct gatcgacgcg accaatcccg acgcgcgcaa atggttctgg
      541 gaccacgcgc gcgacaatat cctcagccac ggcttcgact atccgtggct ggacgagacc
      601 gagccggacc tcgtgccgga cggctctttc ttctccatcg gctccggcga ccgctatcac
      661 aacgtcttcc cgctgctgca cgtcgagggc gtggccgacg gcatgcgcca gtggaagccg
      721 gacaagcgcg tgctgatcct gtcgcgcgcc gcctatctcg gctcgcaacg caccggcgcc
      781 ctgttctggt cgtcggacat caatccgtcg tgggaagcgc tgcggcgcca gattccgacc
      841 gggctgaaca tgaccgcgtc cggcatcgcc tactggggca acgacatcgg cggctggcaa
      901 tggctgccgc agaccacgac cgcgacgaag ccgccgctgc tcgacccgtc cgacgcgcgc
      961 gacgtggtcg gccagaacaa cgactatccc gagctgctga cgcgctggtt cgagtacgga
     1021 accttcctgc ccaccttgcg cctgcacggc gaccgcaagc acacggaaat ctgggccttc
     1081 ggtcatgagg ccgaggcgat catggccaga tacgacaagc tgcgctacga actgatcccg
     1141 tacatctatt ccagcgccaa acacacctac gacaccggcg ccccgttcat gcgcgcgctg
     1201 tggatggact tccccaacga tccgaacgtc agcgacatcg gcacggaata catgttcggc
     1261 ccggccttcc tggtcgcgcc ggtcaccgag cagggccaga ccgaaaagtc cgtctatctg
     1321 cccgccggtt cggactggta caattactgg accaaccaaa agctgaccgg cggccagtgg
     1381 gtcaagaccg ccgccccgat cgaccagatc ccggtcttcg tcaaggcggg ctcgatcgtg
     1441 ccggtcggtt cggacatccc gtccaccgcc acgaagcaga ccatcaccgc cctcaaggtc
     1501 tatccgggcg ccaatggcgg cttcagcctc tatgacgacg acggccacac ctacgattac
     1561 gaaaccggca agggcgtgac ggcctcgcaa ctgacctggg acgacgcgac gggcaaactg
     1621 tccgccaccg gcgccctggc caagagcgcg gccggcctcg tgcaggtgat cgggaaatag
     1681 ggggatcggg cggcgcgcgc cgcccgattt cgcgctgttg gcgacgccct gaatcttggg
     1741 ccgaaacgcg gtctggccca cgcggccgac cctgttcgga tcgtatggcg ttatggtaat
     1801 atcggcgacg gatttacgtc ctgcgggtgt taaacccgta tcgttccctt ttcgccagac
     1861 gccgtcatga cgctcgaccc cgacaaacgc aaacgcctgt accgccgcgt ggcggaggag
     1921 atcatccgcg acgtgcgcgg cggcgtgtac aaggtcggcg cgaaactgcc cgccgagcgc
     1981 gatctggccg acagcatgga cgccagccgc accaccatcc gcgaagcgat catcgcgctg
     2041 gaaatgctgg gcgtcgtcga ggtgcgccac ggcgcgggcg tgttcgtcac cggcgaaggc
     2101 atcgccgcca ccagcgccta tgcgacgcag gatctcaatg tcggcgcctt cgagctgatc
     2161 gaggcgcgca tggtgatcga gagcgaaacc gccgccatcg ccgcccgcgt cgccaccgac
     2221 aaggacgtcg aacaactggc cgaactggtc cgggtgatgg agacgggcca gcccgccgcc
     2281 atcgagcagg ccgaccgcga atttcacctg ctgatcgcgc gcatcaccga gaacggcgcg
     2341 ctgatcgaca cggtgcgcaa tctgtggaac ctgcgcgcgg cctcgccgct ggcctcggcg
     2401 atcatcagcc gcgcccacga ttccggcctt gaggcgcgca cgcgcgaaca tcgccccatc
     2461 ctcgacgcga tccgcgcccg cgaccccgcc gccgcccgca acgccatgcg cgaacatatg
     2521 cagtgcgtac gcgactatgt gctggaggcg accgagatgg cggagatcga aagcctgcgc
     2581 aaccagttgc gcagccagcg cgacgcctgg gccagccgca ccgcgccgta aacgccgaaa
     2641 acgccgggac cgaagcatcg ccggaacggg accagcccgt cccggcatgc ggctctgcac
     2701 gcccgctcca tgccctccga acagcgataa aaaagccggc gcccaaactc ggcgccggcc
     2761 atgaaagtcg gtattggaga aaaaaagcat cagacagatc aacgccaatc ccgcttgcag
     2821 gatcgatgat gagccgcggc ccgatcgctt agcgctaagc ggtccagcca cttgcccgac
     2881 acgaatgatt gtttatgatc aaatccgccg agtcaaggcc aaaaatggcg aattatgggc
     2941 gtatcgtgat catattaaga tgatgaagcg ataaaccctt gatacatgac aaatacgcca
     3001 aattcttcat aagtacgtat tttcagaaaa aatcatagac gagtcaataa agcccatatc
     3061 cgcaattctt tataaccgtt ctcttaagat aaattttcac aaatatataa agccgtatat
     3121 ttgaacgcgc ttcaacttaa gacgattcgc cggttgaatc cgatgtgacc tcggaattac
     3181 cacgctgcgg cgcgggccag agggcggttc ggcgtcggaa tcgcaaaaaa tcgccgtaat
     3241 ccggtcaaaa agtatcattt atgccacaat ggattcgcaa ttaggacgaa aacacgacct
     3301 tttgtgcgca attatgcgca aagaggcttg atatgtcatt ttcaatcata caaaactgcc
     3361 gccagcagag agagactgcg ggaggctcgc cgccctgaag cgcgcgcaac cgcgcttcgc
     3421 cgccaatcac cgcccaggtg gttggaccac ggcaagccgc gatggacgcc tcccccggac
     3481 cgatccgccc caagagccgc cgccacacgg ctcccgccaa agtcataacg cgtgaaaccg
     3541 cgccaggagg aactgaatga acgacctatc ccgaactcag atcatgcgcc gccgcctgac
     3601 cggcggcacc gcgctggcgc tggtcgcgct tttcgccggc gccagtctgg cccacgccgg
     3661 tctggcccac gcccagacgg cggcccggaa cagccaatcc accgcgaaat ccggcaatga
     3721 cgaaaccacg ctggtgatcg tcaccggcac gcgcgcctcg cagcaatcgg ccatcgaccg
     3781 caagaagcgc gccaagacgg cgaccgattc gatcgcggcc gaggacatcg gcaccttccc
     3841 ggacaacaat atcgacgaag ccatttcgcg catcgcgggc atccagctcg accgcggcga
     3901 cttcggcgag ggccagagcg tgaccgtgcg cggccagtcg gcggagaaca accgcgtcga
     3961 gctggacggc gtcggcgtga tgaacacctc cggcggtctg gtcagcggcg cgggcgacgc
     4021 gcgcggcgcg gacctgcgcg aaattcccgc cgacatcgtc aagagtctgg acgtggtcaa
     4081 gggcacgacg gcggccatga ccgaaggctc gctgggcggc tcgatccaca tcaacacccg
     4141 caccggcctc gatttcaaga agccctactt ccagttccgc gccagcgagt cgtccaacac
     4201 gctggacaag aagtggacgc cgtcgtggag cgccatcgcc gcgcgcaagt tcctcaacgg
     4261 ccgcctcggc gtcatcgcca acgtcaacta ttcggaaatc tccaattccg ccgactccga
     4321 acagcccaac acctccggca cggcgggcta ttaccgcaac gtcgatctcg accagtcgcc
     4381 ggaaaagacc ttcacctaca atccggcggt gtcggaaatg gtcggcgaca ccaagctgtc
     4441 caattccgac aacaccatgc tggacgtgat caaaaagtcg gcggccgcgc agacgccgca
     4501 ggactgtctg gcggccttcc ccgtgcccgc ctggacgccg acgacgaaag cccccggccc
     4561 ttcgtccaag gccgccgaag ccggttacga gctgcaatcg tgcctgaacc agtggaacga
     4621 ctatacgccg tcgctga
//
LOCUS       DASVLL010000107         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_328256,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000107 DASVLL010000000
VERSION     DASVLL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328256"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VF402_01785"
     CDS             <1..1432
                     /locus_tag="VF402_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799049.1"
                     /translation="RSVTDVFYNEMSDTDLTDAHKTRLNLLARVSEFQVVRPELAADQ
                     FTPYYPCDDGTQYFSTADVNSMNAPGDPVDLNLAFLYMAYNHQRELDRFETALPNWAA
                     IGLNWYFMSMLVHKDRVEVGRPYPRIAAAYDRRSGTTIDLTQERKRIPFAAVMAGRPL
                     GANRQTEVALASWVMVAYLMNDPARRAKFIDFIHRVQKGETPDDAYAAAIGFPPATYE
                     AAMDAYMDKGVPVAAYTLTAATDADVIVTKLPRFKTALPLLDAATRACPSAAYAADLE
                     KRLGEEAARQPDDVMAQEALARARIRAGHAAAALPWLKSRVAAVPDDFDARLLLGQAW
                     LDVAKNSSGEARASGFRAARNALGEAYKLDPASAPALFYFALAHADLPDYPDDNTLKA
                     LDLAQSYSGGHYEDYKAEFQLRRGDYDGALKSVSEIRDHTAPNQKKALALIAPLYDAL
                     AAKRPAAEILPLFDAVHRYHWETEKP"
     gene            complement(1495..2112)
                     /gene="rpsD"
                     /locus_tag="VF402_01790"
     CDS             complement(1495..2112)
                     /gene="rpsD"
                     /locus_tag="VF402_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7799050.1"
                     /translation="MSKRHSAKYKLDRVMGENLWGRPKSPVNKRSYGPGQHGQRRKSK
                     TSDFGLQLKAKQKLKGYYGNITEKQFLRTYEEASRRKGNTSEILISLLESRLDALVYR
                     AKFVPTVWAARQFVNHGHVLVNGKRVNIPSYRVKPEDVVEVRERSRNMALVLEALQSP
                     ERDVPDYIELDAKGMKAKFLRLPELAEVPYPVKMEPNLIVEYYSS"
BASE COUNT          390 a          734 c          681 g          401 t
ORIGIN      
        1 tcgcagcgtc acggacgttt tttacaacga gatgagcgac acagacctga cggacgcgca
       61 taaaacgcgc ctcaatctgc tggcgcgcgt cagtgaattc caggtggtcc gcccggagct
      121 ggccgccgac cagttcaccc cctactaccc ctgcgacgac ggcacgcaat atttttccac
      181 cgccgacgtg aacagcatga atgcgcccgg cgatccggtg gacctgaacc tggccttcct
      241 ctacatggcc tacaatcacc agagggaact cgatcgtttc gagacggccc tgcccaactg
      301 ggccgcaatc ggactcaact ggtatttcat gtcgatgctg gtccacaagg acagggtcga
      361 ggtcgggcgc ccctatccgc gcatcgccgc cgcctacgac cggcgctcgg gaacgacgat
      421 cgatctgact caggaacgca aacggattcc cttcgccgcc gtcatggcgg gacgccctct
      481 cggcgccaat cgtcagaccg aggtcgcgct ggcctcatgg gtcatggtcg cctatcttat
      541 gaacgacccg gcgcggcgcg ccaaattcat cgatttcatc catcgcgtcc agaagggcga
      601 gacgcccgac gacgcctacg ccgccgccat cggctttccg cccgcgacct atgaggcggc
      661 gatggacgct tacatggaca agggcgtgcc cgtcgccgcc tacacgctca ccgccgcgac
      721 ggacgccgac gtcatcgtga cgaaactgcc gcgctttaaa acggcgctgc ccttgctcga
      781 cgcggcgacg cgcgcgtgtc caagcgcggc gtacgccgcc gatctggaaa agcgcctcgg
      841 cgaggaagcc gcccggcagc cggacgacgt catggctcag gaagccctgg cccgcgcgcg
      901 cattcgcgcc ggtcacgccg ccgccgccct gccctggctg aaaagccgcg ttgcggccgt
      961 tcccgacgat ttcgacgccc ggcttttgct ggggcaagcc tggctcgacg tggcgaaaaa
     1021 cagttcgggc gaggcgcgcg cctctggctt cagggcggcg cgcaacgcct tgggcgaagc
     1081 ctataaactc gatccggcct cggcgcccgc cctgttctat ttcgcgctcg cccatgccga
     1141 cctgccggac tatcccgacg acaacacctt aaaggcgctc gatctggcgc aaagctattc
     1201 cggcggtcat tacgaggatt acaaggccga atttcagctc cggcgcggcg actatgacgg
     1261 cgcgctgaaa agcgtcagcg agattcgcga ccataccgcg cccaatcaga aaaaggcgct
     1321 ggcgctgatc gcgccgctct atgacgccct ggccgccaaa cgcccggcgg cggagattct
     1381 gccgctgttc gacgcggtcc atcgttacca ctgggaaacc gagaagccct gacggaaaaa
     1441 gccccggaag atcgctcttc cggggctttt cttttatcgg ctgcggcgcg aagcttacga
     1501 cgaatagtat tcgacgatca ggttcggctc catcttgacc gggtacggca cttcggccag
     1561 ttcgggcagg cgcaggaact tcgccttcat gcccttggcg tccagctcga tatagtccgg
     1621 cacgtcgcgc tcaggcgatt gcagggcttc gaggacgagg gccatgttgc gcgaacgctc
     1681 gcgcacctcg accacgtcct ccggcttgac gcggtaggag gggatgttga cgcgcttgcc
     1741 gttgaccagc acgtggccgt ggttgacgaa ctgacgcgcc gcccagacgg tcggcacgaa
     1801 cttggcgcgg tacaccagcg cgtcgaggcg cgattccagc agcgagatca ggatttccga
     1861 ggtgttgccc ttacggcgcg acgcctcttc gtaggtgcgc aggaactgct tttcggtgat
     1921 gttgccgtaa tagcccttca gcttttgctt ggccttgagc tgcaggccga agtccgaggt
     1981 cttggacttg cggcgctggc cgtgctggcc ggggccgtag gagcgcttgt tgaccgggga
     2041 cttgggacgg ccccacaggt tttcacccat gacgcggtcg agtttgtact tggcgctgtg
     2101 gcgcttggac atgtcgtgcc tttatatcaa ccctggccgg cttcccgcac tctgcggaag
     2161 gcgattgcaa cggcggcgct caggatcacc cgtcatgacc ggtcgg
//
LOCUS       DASVLL010000108         4889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_436082,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000108 DASVLL010000000
VERSION     DASVLL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436082"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF402_01795"
     CDS             <1..212
                     /locus_tag="VF402_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX7799051.1"
                     /translation="SLKAFVDERVRSGGYGSHSEYLRELVRRDEQQAATERLRALIAE
                     GLNSPPGRDWRDLKADLLKRAAAQG"
     gene            209..520
                     /locus_tag="VF402_01800"
     CDS             209..520
                     /locus_tag="VF402_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7799052.1"
                     /translation="MKPARLRQVAERDVEQAFSYYLQEAGPALAVDFIAAVDAALARM
                     AAHPAAGSPRYAEQLDLPCLRSWLVERFPYVALYVECDDHIDVLRVLHQQQDIPAHMQ
                     A"
     gene            complement(517..1446)
                     /locus_tag="VF402_01805"
     CDS             complement(517..1446)
                     /locus_tag="VF402_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX7799053.1"
                     /translation="MTLPLTASPDDAGRLWLDSLKARRFSPRTLEAYGHAFRGYVAHL
                     SGTVAGPLTLEDVVKASSADLRGWMARLRGKTPPLSAQSLAQHLSAVKAFHKWLDLHH
                     DLPNAQIELVRGPRLKTSLPRPLNEDQALGLLNEAEMDTDREAWENARDLAVFLLLYG
                     LGLRISEALSLQAKDAPLGEALRVTGKGGKQRLLPVLPAVRAAVEAYRAQLPYGLQPD
                     DRLFRAKRGGALSPRHVQARVQHLRSRLGLSDRATPHALRHSFATHLLGSGADLRAIQ
                     ELLGHASLSTTQKYTQVDTERLLSAYAKAHPKG"
     gene            complement(1684..2367)
                     /locus_tag="VF402_01810"
     CDS             complement(1684..2367)
                     /locus_tag="VF402_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX7799054.1"
                     /translation="MTHDGGATSIDLDDWQALRRHLVKHVDSLQRDKALLEALGLQPR
                     QRHLIDFGPAALAKLEQRAMKDLDVRRQLELTARANFDAQSETHALTLSLMEARNPSD
                     LARRLNEEARKRFGLIAGVIALEDTAPVPLGWKTLDYGGVDYIVGEDGLSLLGPEGVC
                     RALFDDDVRRIKSAAVLRTAMWREGRPGLVAFGSSDFEGFTADMGAELVAFVARVVER
                     VAERWPVLG"
     gene            2461..4656
                     /locus_tag="VF402_01815"
     CDS             2461..4656
                     /locus_tag="VF402_01815"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7799055.1"
                     /translation="MSRAPHMESLTESLVAKVVVLAPMSEALDYLVPAGMELAPGDHV
                     VAPLGHAKVRGLVVGFDPEADATRANLKPLLQKLDDPPVPDGALHFWLWAANWTLTPP
                     GVFLNGCLRALKSPRPQPRQGYVLTGAEPSRMTKARQAVIDTALAPMGLSELALAAGV
                     STATVTGLEKDGVLRRVDLPRDDAYAPPDPDFAPARLNDGQAAAHELLRMEMARGRFA
                     PVLLDGVTGSGKTEVYLESVADVLRGDPEAQVLILLPEIALTQAVMGRLKARFGVEVA
                     QWHADISNANRRRIWEGVAEGRIRLVVGARSSLFLPYRRLGLIVIDEEHDGSYKQEEG
                     ARYHARDLAVYRARLDGAMAIMASATPSLETLTNAQQGRYAWIRLERRHGTAQLPGIA
                     LIDMKAHPPEKGHWLSDPLVGEMLAALQRREQVMLFLNRRGYAPLVLCKACGEKMTSP
                     RTDSWLVEHRSTGRLVCHLTGFSMKKPKTCPHCGAQDSLIAVGPGVERILEEVQQRFP
                     DARAEVFSSDTTPDPASSAALIHRIERHEIDIVIATQAAAKGHNFPNLTLVGIVDADL
                     GLKGGDLRAAERTFQLLQQATGRAGRKVKPGRALIQTYAPEHPVMQALAHQDRDAFYM
                     YESMARELAKFPPFGRLAALILSAKDQALLNRFARELALFIPNSEGIEVWGPADAPLA
                     LVRGQWRKRFLVRADRNRDLQAFVAHWLAAIKRPSAVRLVVDIDPYSFL"
BASE COUNT          783 a         1621 c         1705 g          780 t
ORIGIN      
        1 attcgctcaa agccttcgtc gatgagcgcg tgcgcagcgg cggttacggt tcgcatagcg
       61 aatacttacg tgaactggtg cgccgcgatg agcagcaggc cgcgacggag cggctgcgcg
      121 cgttgatcgc ggaagggctg aattcaccgc caggtcgtga ctggcgcgat ctcaaagccg
      181 atcttttaaa gcgcgcggcg gcgcagggat gaagcccgcc cgcctgcgtc aggtcgccga
      241 acgcgacgtc gagcaggctt tcagctatta tctacaggaa gccggtccgg ccctggcggt
      301 cgatttcatc gcggcggtgg acgccgcgct ggcgcgtatg gcggcccatc ccgccgccgg
      361 atcgccgcgc tacgccgaac agctcgacct gccgtgcctg cgctcatggc tagtggagcg
      421 ctttccttat gtcgcgctct atgtcgaatg cgacgatcac atcgacgtcc tgcgcgtcct
      481 gcatcagcag caggatattc ccgctcatat gcaggcttaa cccttcggat gcgccttcgc
      541 ataggccgac aagagccgct ccgtatcgac ctgggtgtat ttctgcgtcg tggacaggct
      601 ggcgtgaccc agcaactcct gaatggcgcg caggtcggcg ccggagccga gcaggtgggt
      661 ggcgaaggag tggcgcagcg cgtgcggcgt ggcgcggtcg gacaggccga ggcgcgagcg
      721 cagatgctgc acccgcgcct gcacatggcg cggcgacagc gcgccgccgc gtttggcgcg
      781 gaacagccgg tcatcgggct gcaacccata gggcagttgc gcgcgatagg cctcgaccgc
      841 cgcgcgcacg gcgggcagca ccggcagcag gcgctgtttg ccgcccttgc cggtgacgcg
      901 caaggcctcg cccagcggcg cgtccttcgc ctgtaatgac agcgcctcgg aaatgcgcag
      961 gcccagcccg tacagcagca ggaacacggc cagatcgcgc gcgttttccc acgcctcgcg
     1021 gtctgtatcc atctccgctt cgttcagcag gcccagcgcc tgatcctcgt tcagcggccg
     1081 cggcaggctg gttttcaggc gcggcccgcg caccaattcg atctgcgcgt tcggcaggtc
     1141 gtgatgcagg tcgagccatt tgtgaaaggc cttgacggcg gacaggtgct gggccagcga
     1201 ctgcgccgac aggggcggcg tcttgccgcg taagcgcgcc atccagccgc gcaggtcggc
     1261 cgacgacgcc ttgaccacgt cctccagcgt cagcggcccc gccacggtcc ccgacagatg
     1321 cgcgacataa ccgcgaaagg cgtggccgta ggcttcgagc gtgcgcgggg aaaaacggcg
     1381 cgccttcagg ctgtccagcc agaggcgccc ggcgtcgtcg ggggacgccg taagaggcaa
     1441 tgtcatgaaa atagctcctc tgcgccgggg cggcctccct cctcgtcctt gcgggcgggg
     1501 cggagtggtg agagggctgt ctccttgccg aaccttgggg gggcgtcgag aagggaagcc
     1561 tcagtgacgc agaggagcat ccggataacc ttgaatatag gtaaggttgt gggtcatttt
     1621 aagacgaaaa tgtgcggatt tcatccgcct cgcggtgatt tcacggcgaa ttgaggcgag
     1681 ggctcagccc agcacgggcc aacgctcggc cacgcgctcg accacgcgcg cgacgaaggc
     1741 gaccagttcg gcgcccatat cggcggtgaa gccctcgaaa tcgctggacc cgaaggcgac
     1801 gagaccgggg cggccctcgc gccacatcgc cgtgcgcagc accgccgcgc tcttgatgcg
     1861 gcgcacgtcg tcgtcgaaca gcgcgcggca gacgccttcc ggccccagca gcgacaggcc
     1921 gtcctcgccg acgatgtagt ccaccccgcc atagtcgagc gtcttccagc ccagcggcac
     1981 aggcgcggtg tcctccagcg cgatcacgcc cgcgatcagg ccgaagcgtt tgcgcgcctc
     2041 ctcgttcagg cggcgggcca gatcggacgg attgcgcgcc tccatcagcg acagggtcag
     2101 ggcgtgggtc tcgctctggg cgtcgaaatt ggcccgtgcg gtcagctcca gctggcggcg
     2161 cacgtcgaga tccttcatcg cgcgctgctc cagcttcgcc agcgccgccg ggccgaagtc
     2221 gatcagatgc cgctgacgcg gctgcaggcc cagcgcctcc agcagcgcct tgtcccgctg
     2281 tagcgaatcg acgtgtttca ccagatggcg gcgcagggcc tgccagtcgt cgaggtcgat
     2341 ggatgtcgcg ccgccgtcat gagtcatggg cggcacctta gagtcgccgc gcggcgctgt
     2401 gaagagggtt cgccgcgcgg ccggcgacaa agcggcgatt ttcgtgttag aggcgtcggc
     2461 atgagtcgcg cccctcatat ggaaagcctt acagaaagcc tcgtcgccaa ggtcgtcgtg
     2521 ctggcgccga tgagcgaggc gctggactat ctcgtcccgg cgggcatgga actggcgccg
     2581 ggcgatcacg tcgtcgcgcc gctgggccat gcgaaggtgc gcgggctggt ggtcggcttc
     2641 gatcccgaag cggacgcgac gagggccaat ctcaagccgc tgctgcaaaa gctcgacgat
     2701 ccgccggtcc cggacggcgc gctgcatttc tggctgtggg cggccaactg gacgctgacg
     2761 ccgccgggcg tgttcctgaa cggctgcctg cgcgcgctca aaagcccgcg tccgcagccg
     2821 aggcagggct atgtgctgac gggggccgaa ccgtcgcgca tgaccaaggc gcggcaggcg
     2881 gtgatcgaca cggctttggc gcctatgggt ttgagcgaac tggcgctggc ggcgggggtg
     2941 tcgacggcga cggtgacggg gctggaaaag gacggcgtgc tgcgccgggt cgacctgccg
     3001 cgcgacgacg cctatgcgcc gcccgatccc gatttcgcgc cggcgcggct caatgacggg
     3061 caggcggcgg cgcatgagct tttgcgtatg gagatggcca ggggccgctt cgcgccggtg
     3121 ctgctggacg gggtgaccgg ttcgggcaag accgaggttt atctcgaaag cgtcgccgat
     3181 gtcctgcgcg gcgaccctga ggcgcaggtg ctgatcctgc tgccggaaat cgcgctgacc
     3241 caggccgtga tgggccgcct gaaagcgcgc ttcggcgtcg aggtcgcgca atggcacgcc
     3301 gatatttcca acgccaaccg ccgccgcatc tgggaaggcg tggccgaggg gcgcatccgg
     3361 cttgtcgtcg gggcgcgctc aagcctgttc ctgccctacc ggcgcctcgg cctgatcgtg
     3421 atcgacgagg agcatgacgg ctcctacaag caggaggagg gcgcgcgtta tcacgcccgc
     3481 gatctggccg tctatcgcgc ccggctggac ggggcgatgg cgatcatggc ctcggccacg
     3541 ccgtcgctgg agacgctgac caacgcccag caggggcgct atgcgtggat acggctggag
     3601 cggcggcacg ggaccgcgca actgcccggc atcgcgctga tcgacatgaa ggcgcacccg
     3661 ccggaaaagg gccattggct gtccgatccg ctggtcggcg agatgctggc ggccctgcaa
     3721 cggcgcgagc aggtcatgct gttcctcaac cggcgcggct atgcgccgct ggtgctgtgc
     3781 aaggcgtgcg gcgagaagat gacctcgccc aggaccgatt cgtggctggt cgaacacaga
     3841 tcgacgggcc gcctcgtctg ccacctgacc ggcttttcga tgaagaagcc aaagacctgc
     3901 ccgcattgcg gcgcgcagga cagcctgatc gcggtcgggc cgggcgtcga gcggattctg
     3961 gaggaggtgc aacagcgctt tcccgacgcg cgcgccgagg tgttctcgtc cgacaccacg
     4021 ccggacccgg cgtcctcggc ggcgctgatc caccgcatcg aaaggcatga gatcgacatc
     4081 gtgatcgcca cgcaggccgc ggcgaaaggt cacaacttcc ccaacctgac tctggtgggg
     4141 atcgtcgacg ccgatctggg gctgaagggc ggcgacctgc gcgccgccga gcgcaccttc
     4201 cagcttttgc agcaggcgac cggccgcgcg ggccgcaagg tcaagccggg ccgcgccctg
     4261 atccagacct acgcgcccga acatccggtg atgcaggcgc tcgcgcatca ggaccgcgac
     4321 gccttctaca tgtacgaatc gatggcgcgc gagctggcga aattcccgcc gttcggacgg
     4381 ctggcggcgc tgatcctgtc ggcgaaggat caggccctgc tcaaccgctt cgcgcgcgaa
     4441 ctggccctgt tcatccccaa cagcgagggc atcgaggtct ggggaccggc ggacgcgccg
     4501 ctggccctgg tgcgcgggca atggcgcaag cggtttttgg tgcgcgcgga ccgcaaccgc
     4561 gatctgcagg ccttcgtcgc ccactggctg gcggcgatca agcgccccag cgccgtgcga
     4621 ttggtggtcg acatcgatcc gtatagtttc ctctgacgcc cctctcccct ccacgagggg
     4681 agaggacgag gagcaaggcg cagccgccga tcctcgggtg aggggctgtc ggacgatgga
     4741 gcgtagccgg ctgacagccc ctcaggctgc gcgccccgcc cgtcgccttc gctgcgctcg
     4801 gatctcctcc tctccccgca agcgggggga ggggtcttga atccagcctt caaaaccgcc
     4861 aagtactgcg ccatgacaga ttcccattt
//
LOCUS       DASVLL010000109         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_275383,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000109 DASVLL010000000
VERSION     DASVLL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275383"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VF402_01820"
     CDS             complement(<1..154)
                     /locus_tag="VF402_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEX7799056.1"
                     /translation="MCQPSLYKKTYHATHPDMMQNVSNQDLRDKYLLSGLFEPDAVVL
                     NYTHFER"
     gene            complement(267..1679)
                     /gene="uxaC"
                     /locus_tag="VF402_01825"
     CDS             complement(267..1679)
                     /gene="uxaC"
                     /locus_tag="VF402_01825"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX7799057.1"
                     /translation="MAKPLLVHEDRLFSSDPSQRGIARELYALVKDYPILSPHGHTDP
                     AWFADNHNFGDATELFLAPDHYIYRMLYSQGIAMEDVGVGGRSGPSKADARTAWRLFA
                     KNWRLFRGTPTAMWMSYVFNKIMGFEVQLGEDTADFYFDRIGELLASDAYKPRALFER
                     FNIELLATTEGPTDTLEHHAKIRASGWKGRVVTAYRPDPVIDPEHEDFHAKLDLFADL
                     TGEDVRGWSGYLAAHRKRRDVFMTMGATSTDHGHPTARTANLPRAEAEALFARVLRPD
                     VTAADAELFRAQMLTEMARMSLDDGLTMQIHPGAWRNHNKAVFDTFGRDKGCDIPMQT
                     GYVEELKPLLDAFGNEKNLTVILFTLDETSYARELAPLAGHYPILKLGPSWWFHDSPE
                     GMTRFREQVTETAGFYNTVGFNDDTRAFLSIPARHDVARRIDANFLGRLVAEHRLELD
                     EAAEVIKDLTYTLPKAAYKL"
     gene            complement(1844..>2114)
                     /locus_tag="VF402_01830"
     CDS             complement(1844..>2114)
                     /locus_tag="VF402_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7799058.1"
                     /translation="FASNDEMATGVYRAALEKGLKIPGDLSLVGFDDSPIASWISPPL
                     TTVRMAIPDMGRAAADCLLGEADERLVELESSLVVRGSTSPPAGD"
BASE COUNT          366 a          632 c          727 g          389 t
ORIGIN      
        1 agcgctcgaa atgggtgtag ttcagcacga ccgcgtccgg ctcgaacagg ccggacagca
       61 ggtacttgtc gcgcagatcc tgattgctga cgttctgcat catgtccgga tgcgtggcgt
      121 ggtaggtctt cttgtaaagc gagggctggc acatggggcg aaatatctcc atcgtggccc
      181 gttcgatcag aacgggtcag tttcaatctc tttagagcaa agcggccggt gaaggaaagg
      241 ctttccggcc aatatttttc gacagcctac agtttatacg ccgccttggg cagggtgtag
      301 gtcagatcct tgatcacctc ggcggcctcg tccagttcga gacgatgctc ggccaccaga
      361 cggcctaaga agttggcgtc gatgcggcgg gcgacgtcgt ggcgggccgg gatcgacagg
      421 aaggcgcgcg tgtcgtcgtt gaagccgacc gtgttgtaga agcccgccgt ctcggtcacc
      481 tgctcgcgga agcgcgtcat gccttcgggg ctgtcgtgga accaccacga cgggccgagc
      541 ttcaggatcg gataatgccc ggccagcggc gccagttcgc gcgcatagga ggtttcgtcc
      601 agcgtgaaca ggatgacggt gaggttcttc tcgttgccga aggcgtcgag cagcggcttc
      661 agttcctcga catagccggt ctgcatcggg atgtcgcaac ccttgtcgcg cccgaacgta
      721 tcgaacaccg ccttgttgtg gttgcgccag gcgccggggt gaatctgcat ggtgaggccg
      781 tcgtccagcg acatgcgcgc catttcggtc agcatctggg cgcggaacag ttcggcgtcc
      841 gccgccgtca cgtccggccg cagcacgcgc gcgaacaaag cctcggcctc ggcgcgcggc
      901 aggttggcgg tgcgcgccgt cggatggccg tggtcggtgg aggtcgcgcc catggtcatg
      961 aaaacatcgc gccgcttgcg gtgcgcggcc agatagccgc tccagccgcg cacgtcctcg
     1021 ccggtcaggt cggcgaacag gtcgagtttg gcgtgaaagt cctcatgctc cgggtcgatg
     1081 accggatcgg ggcggtaggc ggtgacgacg cggcccttcc agccggacgc cctgatcttg
     1141 gcgtgatgct ccagcgtgtc ggtcgggcct tcggtcgtgg ccagcagctc gatgttgaaa
     1201 cgctcgaaca gggcgcgcgg cttgtaggcg tcggacgcca gcaattcgcc gatgcggtcg
     1261 aagtaaaaat cggcggtgtc ctcgccaagc tggacctcga agcccatgat cttgttgaaa
     1321 acataggaca tccacatggc ggtcggcgtg ccgcggaaca ggcgccagtt cttcgcgaac
     1381 agccgccacg ccgtgcgcgc gtccgccttg gacgggccgg aacggccgcc gacgccgaca
     1441 tcctccatcg caatgccctg cgaatagagc atccggtaga tgtaatggtc gggggccagg
     1501 aacagctcgg tcgcgtcgcc gaaattgtgg ttgtcggcaa accacgccgg atcggtatgg
     1561 ccgtgcggcg acaggatcgg atagtccttc accagcgcat acagctcgcg cgcgatcccg
     1621 cgctgcgacg ggtcggagga aaacaggcgg tcctcatgca cgaggagggg cttggccatg
     1681 atgcagtacc tgaaaatgaa acgcttggaa tatccctgag gcggcgttgt cgcgcgtggg
     1741 ccgcgctgtc cgtgtcgcgg ttgcttaggt aaccggtgtc aaagactttg acaagggtga
     1801 aacggccctg cgccgaaaat ccgtcgcaga ttcggttgcg gattcagtcc ccggcgggcg
     1861 gactggtcga gccgcgcacc acgaggctgg actccagctc gaccagccgt tcgtccgcct
     1921 cgcccagcag gcagtcggcg gcggcgcggc ccatgtcggg aatggccatg cgcacggtcg
     1981 tgagcggcgg cgatatccac gaggcgatcg ggctgtcgtc gaagccgacc aggctgaggt
     2041 cgccggggat tttcaggccc ttttccagcg ccgcgcgata gacgccggtg gccatttcgt
     2101 cgttggaggc gaag
//
LOCUS       DASVLL010000110         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_328301,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000110 DASVLL010000000
VERSION     DASVLL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328301"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VF402_01835"
     CDS             complement(<1..991)
                     /locus_tag="VF402_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7799059.1"
                     /translation="MLRHNTEAERTHPAKMLSLLKTPRAEFTLDAPSSEDGVAVLRLK
                     GRWVITRMDGVTARLRAATRKYDKVRVEAEELEALDSAGALSLHLALDGRLDGAAFAP
                     DSKFARLFDLVGARAPDLRDWEQDNPQARRAWWRPVYALLVWAGMWVARFARGFMAQT
                     AFLGHVTTVALNSLMRPARIRWPALINIMQRAGIDAIPIVALTSLFIGAVIGFIGVLQ
                     LQQYGGGIFAIDMVAVFSLREFAPLITAILLAGRSASAFAAEVGSMKMNQEIDAMRVM
                     GIEPYEALVLPRILGLVIMSPFITMIAALAAMFGGMLAIWSSLGAGPATFLQRL"
     gene            complement(1072..1335)
                     /locus_tag="VF402_01840"
     CDS             complement(1072..1335)
                     /locus_tag="VF402_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HEX7799060.1"
                     /translation="MSQLIEETIEALLSQARDGESISPNDVAKVLNPDGWRRELPKIR
                     AVVVGLTRRGRLEMLRKGRPTEPEEVRGVYRIRKPAPGADAGR"
     gene            1362..1814
                     /locus_tag="VF402_01845"
     CDS             1362..1814
                     /locus_tag="VF402_01845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7799061.1"
                     /translation="MTERQIETLSEGEFAGWSREVNREGFNGLVGAYYYQADDPANLK
                     VAFKAGKEHLNGGGTVHGGCLLTLADTSMFIFSLPHLQDGGAVTVQLDAQFLSPGREG
                     EVIVATGEVTRAGRSLVYVRGQLTCGERLIMTYSGVMARIPTRPSGDG"
BASE COUNT          357 a          650 c          625 g          324 t
ORIGIN      
        1 gcagccgttg caggaaggtg gccggacccg cgcccaggct ggaccagata gccagcatgc
       61 cgccgaacat cgcggccagc gccgcgatca tggtgatgaa gggcgacatg atgacgaggc
      121 cgaggatacg cggcagcacc agcgcctcat acggctcgat gcccatgacg cgcatggcgt
      181 cgatctcctg attcatcttc atcgagccga cctcggcggc gaaggccgaa gccgagcgcc
      241 cggccagcag gatggccgtg atcagcggcg cgaattcgcg taaggagaac accgccacca
      301 tgtcgatggc gaagatgccg ccgccgtatt gctgaagctg caacacgccg atgaagccga
      361 tcaccgcgcc gatgaacagg ctggtcaggg ccacgatcgg gatcgcgtcg atgcccgcgc
      421 gctgcatgat gttgatcagg gcgggccagc ggatgcgcgc cgggcgcatc aggctgttca
      481 gggcgaccgt cgtgacgtgg cccaggaacg ccgtctgcgc catgaagccg cgcgcgaacc
      541 gcgccaccca catgccggcc cagaccagca gggcgtaaac cggccgccac caggcgcggc
      601 gcgcctgcgg attgtcctgc tcccagtcgc gcaggtccgg cgcgcgcgcg ccgaccagat
      661 cgaacaggcg cgcgaacttg ctgtcgggtg cgaaggccgc gccgtccagc cgcccgtcca
      721 gcgccagatg caggctcagg gcgcccgcgc tgtccagcgc ctccagctct tcggcctcga
      781 cgcgcacctt gtcgtatttg cgcgtcgcgg cgcgcagccg cgccgtcacg ccgtccatgc
      841 gcgtgatcac ccagcgccct ttcaggcgca gcaccgcgac gccgtcctcc gacgatggcg
      901 cgtcaagcgt aaattcggcg cgtggcgttt tcaaaaggct cagcatcttg gccgggtgcg
      961 tccgctctgc ttcggtatta tgccgcagca tacatcgggc taattgataa aatggcaata
     1021 gctaagacga aaaaagcgta ccggactgtt cactttttcc ggcgcaatat gtcatcggcc
     1081 cgcgtcggcg cccggagcgg gtttgcggat gcgatagacg ccgcgcacct cttccggctc
     1141 ggtcggacgg cccttgcgca gcatctccag ccgcccccgg cgcgtcaggc cgacaacgac
     1201 ggcgcggatt ttcggcagtt cgcgccgcca gccgtccggg ttcaaaacct tggccacgtc
     1261 gttggggctt atggactcgc cgtctcgcgc ctgcgataag agcgcctcga tcgtttcctc
     1321 gatcagttgc gacataattt tcagtatccc ggagttggac catgacggaa cgccagatcg
     1381 aaaccctgag cgaaggcgaa ttcgccggct ggtcgcgcga ggtcaaccgc gaaggcttca
     1441 acggtctggt cggagcctat tactatcagg ccgacgatcc cgccaacctc aaagtggcgt
     1501 tcaaggccgg aaaagagcat ctgaacggcg gcggcacggt gcatggcggc tgtcttctga
     1561 ccctggccga cacctcgatg ttcatctttt cgctgccgca tcttcaggac ggcggcgcgg
     1621 tgacggtgca actggacgcg cagttcctgt cgcccggacg cgaaggcgag gtcatcgtcg
     1681 ccaccggcga ggtgacgcgc gcgggcagga gtctcgtcta tgtgcgcggg caactgacgt
     1741 gcggcgagcg cctgatcatg acctattccg gcgtgatggc ccgcatcccg acccgcccca
     1801 gcggcgacgg ctgagcggaa gcttcggatt ttacgaattt tacaagagat gccgccccgg
     1861 tcggactcga aacgacacac cttgcggaaa ctacatattt tacgagagat ggtgcccccg
     1921 gtcggactcg aaccgacaca ccttgcggta ctcgat
//
LOCUS       DASVLL010000111         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_6248, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000111 DASVLL010000000
VERSION     DASVLL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6248"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF402_01850"
     CDS             <1..215
                     /locus_tag="VF402_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799062.1"
                     /translation="FVVLAAKELGDGVRTVTVVNDARNLERIRRVRPDLIIAPTILGG
                     ELLAMTLSGERPTGDDFLNRLLQGGI"
     gene            218..1048
                     /gene="fdhD"
                     /locus_tag="VF402_01855"
     CDS             218..1048
                     /gene="fdhD"
                     /locus_tag="VF402_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX7799063.1"
                     /translation="MEDIERPPGEATRDAPVRRLGFGATPDADLVRPVAIEAPVSIEI
                     GGVAYAVMMATPADLRDYALGFALSEGLVASADEVRSLSAAPAQGGWAVRMRLPPERT
                     EAVYERARRRVSESSCGLCGMDNIETVLRPLPPVASRLATDGAAIARAVACLPDHQPL
                     GRRTGAAHAAAFCSAQGEILHVREDVGRHNALDKLIGALAWGGIDPARGFFLLTARCS
                     YELVEKAARAGCPLLVTISAPTSLAVERAEAAGLTLVSLARHDSALVVCDPAGNIRKA
                     "
     gene            1135..1311
                     /locus_tag="VF402_01860"
     CDS             1135..1311
                     /locus_tag="VF402_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799064.1"
                     /translation="MCLDPVAEGLIVSAAIVSDPDKPMVYVIWDDDRYPILRVDVEEL
                     EVVPGASSAISAGY"
     gene            1347..>1904
                     /locus_tag="VF402_01865"
     CDS             1347..>1904
                     /locus_tag="VF402_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX7799065.1"
                     /translation="MPRTDPPPAAPAATDNHYFRFHAQNAPLQSAFGNDWFALRAEAF
                     ARFFGTPKFLIGQTLFVAAWVLVNATGLVKFDVYPFILLNLAFSLQSAYAAPLILLAQ
                     TRQADRDKVTAEADAKHREDLAMASQKRQAQVETQTAMLLELVQQNTQLTEAVKDLIE
                     RVEGLTKDMHKALCLSDGPNETPADA"
BASE COUNT          309 a          638 c          649 g          308 t
ORIGIN      
        1 cctttgtcgt cctcgccgcc aaggagctgg gggacggcgt caggacggtc accgtggtca
       61 acgacgcgcg caatctggag cgcatccggc gcgtgcggcc ggacctgatc atcgcgccga
      121 cgattctggg cggagaactg ctcgccatga ccctgagcgg ggagcggccc acgggcgacg
      181 attttctgaa tcgccttctt cagggcggca tttgaccatg gaggacatcg aacggccacc
      241 gggcgaggcg acgcgggacg cgccggtccg ccgcctgggc ttcggcgcca cgccggacgc
      301 ggacctcgtg cgcccggtcg cgatcgaggc gccggtttcg atcgagatcg gcggcgtggc
      361 ctatgcggtc atgatggcga cgcctgcgga cctgcgcgac tatgcgctcg gctttgcgct
      421 gagcgaaggg ctggtggcgt cggcggacga ggtgcggagc ctttcggcgg cgccggcgca
      481 gggcggctgg gccgtgcgca tgcgcctgcc gcccgaacgg acggaggccg tctatgagcg
      541 cgcccgcagg cgcgtatcgg aaagcagttg cggcctgtgc ggcatggaca atatcgaaac
      601 cgtgctgcgt cccctgccgc ccgtcgcgtc gcgcctcgcg acggacggag cggccatcgc
      661 gcgcgctgtg gcctgcctgc cggaccatca accgctgggc cgccggacgg gcgcggcgca
      721 cgcggcggcg ttctgctccg cgcagggcga gattctgcat gtgcgtgagg atgtcggacg
      781 gcacaatgcg ctcgacaagc tgatcggggc gctggcctgg ggcgggatcg atccggcgcg
      841 cggctttttc cttttgacgg cgcggtgcag ctatgagctc gtcgaaaagg cggcgcgcgc
      901 agggtgcccg ctgctggtga ccatttccgc gccgaccagc ctggccgtcg aacgcgctga
      961 ggccgcgggg cttaccctgg tgtcgctggc gcgtcacgat tcggcgctcg tcgtttgcga
     1021 cccggcgggc aatatccgca aagcctgaca taggaggacg ctcaaatggc gaacagacgc
     1081 cggaccttta gcgacgcgga acgggtttcc cacgaggtct ggggactggg caaggtgtgc
     1141 ctggaccccg tggccgaggg cctgatcgtg tcggcggcca tcgtctccga cccggacaag
     1201 cctatggtgt atgtgatctg ggacgacgac cgctacccga tcctgcgggt cgatgtcgaa
     1261 gagctggagg tcgttcccgg cgcttcgagc gcgatttcgg ccggctattg agttaagctt
     1321 ccgtcgtatc agagaggaga tcccccatgc cccggaccga tccgccgccc gccgcccctg
     1381 cggcgaccga caaccattat ttccgcttcc acgcgcaaaa cgccccgctg caatcggcct
     1441 tcggcaatga ctggttcgcg ttgcgcgccg aagccttcgc gcgctttttc ggcacgccga
     1501 aattcctgat cggccagacg ctcttcgtcg cggcctgggt tctcgtcaac gccacggggc
     1561 tggtgaaatt cgacgtctat ccgttcattc tgctgaacct ggccttcagc ctgcaatccg
     1621 cctacgccgc gccgctcatc ctgctggcgc aaacccggca ggccgatcgc gacaaggtga
     1681 ccgccgaagc cgacgccaaa caccgcgaag acctcgccat ggccagccag aaacgccagg
     1741 cccaggtcga aacgcaaacc gcgatgctcc tcgaactggt ccagcaaaac acgcaactga
     1801 ccgaagccgt caaggatctg atcgaacgcg tcgaaggcct gaccaaggac atgcacaagg
     1861 ccctgtgcct gtcggacggc ccgaacgaaa cgcccgcaga cgcg
//
LOCUS       DASVLL010000112         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_328327,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000112 DASVLL010000000
VERSION     DASVLL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328327"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2255)
                     /gene="glnD"
                     /locus_tag="VF402_01870"
     CDS             complement(<1..2255)
                     /gene="glnD"
                     /locus_tag="VF402_01870"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX7799066.1"
                     /translation="MSDLLPMLNPTPSGAPAYRIDGAQLRQALTAAYEDSSGDALDLR
                     RRAVILLKDAWDQARAALYVRLDEQKGGAETARALARCADEIVSALWDFTLTHVYRAR
                     NPTEGERLALLAVGGYGRGELAPFSDLDLLFLRAWKETSHSESVIEFILYTLWDLGLK
                     VGHASRTVDEAIKYAKGDHTIMTALLEHRRIAGDKALVQDLDDKLARDGLRTAAFDFV
                     AAKLEERDARHLKAGVTRFVVEPNLKEGKGGLRDLHTLFWIARYLSGENPKIKGGWGA
                     LDALLTDKERGVFKRAFDFLWKVRILVHIVSGRAEERLTFDLQPEIARRMGFLDRNGE
                     PNVERFMRRYFITAKEVGALTRAFCAKLEEQRAKPLARLSSLIQSGLMNLTRADHPGF
                     TVVGGRLSVQSPDIFVKSPVAMFLLFKMADRLELDLHPDAFTAIARHLDLVTPSLRRN
                     PEAAKLFLDLLARGKNPYRTLSLMAETGLLGRYIPEFGRIVAQTQFNMYHAYTVDEHT
                     LRAIDIIHGIDTGRYKDEHPLSSSIMPHLADKETLYLAMLLHDTGKGGEDGQEIGGEI
                     AARKACERLGLEDWKVEQVAWLVRHHLILSDYAQKRDVSDPETVAAFARIVETPERLR
                     QLLILTVADIRAVGPGVWNGWKGQLMRDLFAATESAFRGGRNSDALIGIRERLNEGAS
                     ARRVQLSENNADMAAWLETMDDTYLFAFTPEQVRQHAALVWSSFGSEPAVKAHIDAQR
                     NATAFSISADDR"
     gene            complement(2274..>3813)
                     /gene="mutS"
                     /locus_tag="VF402_01875"
     CDS             complement(2274..>3813)
                     /gene="mutS"
                     /locus_tag="VF402_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7799067.1"
                     /translation="IGETLAARLNDACHRADPDLIASCPAEIEAVIAALIPSPQLSAL
                     RDRLARAVVDEPPLSLRDGGYIRSGFHADLDEARSLRDDSRQVILALEQRLAAETGVA
                     IRIKFNNVLGYFIELSARLAEQLQAQDAQKQFIHRQSLANQVRFVTTELVELDGRIQR
                     AAAQGLALEQEIFESLRDQVRDNAAGIQAAHDAICALDVACANAHWAEDHEATRPVID
                     DSTVLEISRGRHPVVAAALKAQGKPFTPNDVELDASGETAPRLSLVTGPNMAGKSTFL
                     RQNALLIIMAQAGLFVPAKAMRLGVVDRLFSRVGAGDDLAQGRSTFMMEMVETAAILS
                     QATQRSFVVLDEIGRGTATYDGLAIAWACTEHLHEVTGARTLFATHYHELSDLEQRLG
                     HVSNISLTAREHNGELIFLHEAKKGAADRSYGVQVARLAGMPGPVVARARDILSRLEA
                     DNHTELRLDDLPLFSQGAPAAAVTRAPSKAESLLKSLNPDDLSPKEALDLIYNLHKWV
                     KEGG"
BASE COUNT          593 a         1235 c         1344 g          641 t
ORIGIN      
        1 cggtcgtcgg ccgagatcga gaaggcggtg gcgttgcgct gggcgtcgat atgcgccttg
       61 accgccggtt cggagccgaa gctggaccag accagcgcgg cgtgctggcg cacctgttcg
      121 ggcgtgaagg cgaacaggta ggtgtcgtcc atcgtctcca gccacgcggc catgtcggcg
      181 ttgttctcgc tgagctggac gcggcgcgcg gaggcgcctt cgttgaggcg ctcgcggatg
      241 ccgatcagcg cgtcggaatt gcgcccgccg cggaacgcgc tctcggtggc ggcgaacagg
      301 tcgcgcatca actggccctt ccagccgttc cacacgcccg gcccgacggc gcggatatcg
      361 gccacggtca ggatcagcaa ctggcgcagg cgctccggcg tctcgacgat gcgcgcgaag
      421 gccgcgaccg tttccgggtc ggagacgtcg cgcttctgcg catagtcgct gaggatcagg
      481 tggtggcgca ccagccaggc gacctgctcg accttccagt cctccagccc cagccgctcg
      541 cacgccttgc gcgcggcgat ctcgccgccg atctcctgcc cgtcctcgcc gcccttgccg
      601 gtgtcgtgca gcagcatggc cagatagagg gtctccttgt cggccagatg cggcatgatc
      661 gatgaggaca gaggatgctc gtccttgtaa cgcccggtgt cgatgccgtg gatgatgtcg
      721 atggcgcgca gcgtgtgttc gtccaccgta taggcgtggt acatgttgaa ctgggtctgg
      781 gcgacgatgc ggccgaattc ggggatgtag cgccctaaaa gccccgtctc cgccatcagg
      841 ctcaaggtgc ggtacggatt cttgccgcgc gccagaaggt cgagaaacag cttcgccgcc
      901 tccgggttgc ggcgcaggga cggcgtgacc agatcgagat ggcgcgcgat ggcggtgaag
      961 gcgtccgggt gcaggtccag ctccagccgg tcggccattt tgaacagcag gaacatggcc
     1021 acgggcgact tgacgaagat gtccggcgac tggacgctca gccgcccgcc gaccacggtg
     1081 aagcccggat ggtcggcgcg cgtcaggttc atcagcccgg actggatcag gctggacagc
     1141 cgcgccagcg gcttggcgcg ctgctcctcc agcttggcgc agaaggcgcg cgtcagggcg
     1201 ccgacctcct tggcggtgat gaagtagcgg cgcatgaagc gctcgacatt gggttcgccg
     1261 ttgcggtcta aaaagcccat ccgccgcgcg atttccggtt gcaggtcgaa ggtcagccgc
     1321 tcctcggcgc gcccggagac gatgtggacg aggatgcgca ccttccacaa aaagtcgaag
     1381 gcgcgcttga agacgccgcg ctccttgtcg gtcagcaggg cgtcgagcgc gccccagccg
     1441 cccttgatct tcgggttttc gcccgacagg tagcgggcga tccagaacag ggtgtgcagg
     1501 tcgcgcagcc cccccttgcc ctccttcagg ttcggctcga ccacgaagcg cgtgacgccc
     1561 gccttgaggt ggcgcgcgtc gcgctcctcc agcttggccg cgacgaagtc gaaggcggcg
     1621 gtgcgcaggc cgtccctggc cagcttgtcg tcgagatcct gcaccagcgc cttgtcgccc
     1681 gcgatgcggc gatgctctaa aagcgcggtc atgatggtgt ggtcgccctt ggcgtatttg
     1741 atcgcctcat cgaccgtgcg cgaggcgtgg ccgaccttca ggcccagatc ccacagcgta
     1801 tagaggatga attcgatcac cgattcggag tgcgaggttt ccttccacgc gcgcaggaac
     1861 agaaggtcga gatcggaaaa cggcgccagc tcgccgcgcc cgtagccgcc gaccgccagc
     1921 agggccagcc gctcgccctc cgtcgggttg cgcgcgcgat agacgtgggt gagcgtgaag
     1981 tcccacaggg ccgagacgat ctcgtcggcg cagcgggcca gcgcgcgcgc cgtctcggcg
     2041 cccccctttt gctcgtcgag gcggacgtac agggcggcgc gcgcctgatc ccacgcgtct
     2101 ttcagaagga tcacggcgcg gcggcgcagg tcgagcgcgt cgccgctcga gtcctcgtaa
     2161 gcggcggtca gggcctgacg cagttgcgcg ccgtcgatcc ggtaagccgg agcgccggaa
     2221 ggggtcgggt tgagcatggg gaggagatcg ctcatggagg gacgcttgaa aacctaaccc
     2281 ccctccttaa cccatttgtg taaattatag ataaggtcaa gggcctcctt cggacttaaa
     2341 tcgtccgggt tgaggctctt gagcagactt tcggccttcg acggcgcgcg cgtgacggcg
     2401 gcggccggcg cgccttgcga gaacagcggc aggtcgtcga gccgcagttc ggtgtggttg
     2461 tcggcctcca gccggctcag gatgtcgcgc gcgcgcgcca ccaccggacc gggcatcccg
     2521 gccagccgcg ccacctgcac gccgtaggag cggtccgccg cgcccttctt cgcctcgtgc
     2581 aggaagatca gctcgccatt atgctcgcgc gcggtcaggc tgatattgct gacgtggccg
     2641 aggcgctgtt cgaggtcgga cagttcgtga taatgggtgg cgaacagggt gcgcgcgccg
     2701 gtgacctcat gcagatgctc ggtgcaggcc caggcgatgg cgaggccgtc ataggtggcc
     2761 gtgccgcgcc cgatctcgtc cagcaccacg aaggagcgct gcgtcgcctg cgacagaatg
     2821 gcggcggtct cgaccatctc catcatgaag gtcgagcggc cctgcgccag atcgtcgccc
     2881 gcgccgacgc gcgagaacaa acggtcgacc acgcccagcc gcatggcctt cgccgggacg
     2941 aacagcccgg cctgcgccat gatgatcaga agcgcgttct ggcgcagaaa ggtcgattta
     3001 ccggccatgt tcggcccggt gacgaggctc aggcgcggag cggtttcgcc ggaagcgtca
     3061 agctcgacgt cgttgggcgt gaacggcttg ccctgcgcct tcagggcggc ggcgacgacc
     3121 gggtggcgtc ctctcgaaat ctccagaacc gtggaatcgt ctatgaccgg ccgcgtcgcc
     3181 tcgtgatcct cggcccagtg cgcgttggcg caggcgacgt cgagcgcgca gatggcgtcg
     3241 tgcgcggcct gaatcccggc ggcgttgtcg cgcacctgat cgcgcaggga ctcgaaaatc
     3301 tcctgctcca gcgcaagacc ctgcgccgcc gcgcgctgga tgcgcccgtc cagttcgacc
     3361 agttcggtgg tgacgaaacg cacctggttg gccaggctct gacggtggat gaactgcttt
     3421 tgcgcgtcct gcgcctgtag ctgctcggcc agccgggccg acagctcgat gaagtagccg
     3481 aggacgttgt tgaacttgat gcggatggcg acgccggttt cggcggccag tctttgctcc
     3541 agggcgagga tgacctggcg gctgtcgtcg cgcaagctcc gcgcctcgtc gagatcggcg
     3601 tggaagcctg agcggatata gccgccgtcg cgtaaggaca gcggcggctc gtcgaccacg
     3661 gcgcgcgcca gccggtcgcg cagggcgctg agttgcggcg acgggatcag ggccgcgata
     3721 acggcctcga tctcggccgg gcacgaggcg atcaggtcgg gatcggcgcg atggcaggcg
     3781 tcgttgagcc gggcggcgag cgtttcgccg atg
//
LOCUS       DASVLL010000113         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_275489,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000113 DASVLL010000000
VERSION     DASVLL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275489"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VF402_01880"
     CDS             complement(<1..1276)
                     /locus_tag="VF402_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7799068.1"
                     /translation="MLQTRSDEISTAQARLHEVVRAAKAAGADAAEAVFATSRALSIG
                     VRKGEVETVERDETGDLGLRVFIGRRQAVVSVSDFSPAALSRLVERAVAMARLAPEDP
                     YAWLADPARLYRASDDAPDLDLYDTAEPGARSLQDRALAIEAAGLALSARLQSDAANA
                     GYTQNRWLMLTSDGFEGAHRSTLFFQSGRFIASGGDGQMERDGEGRSTRFLSDLPDVA
                     DIGRTGAERALAALGARKLDTQKTAVIFDQRVARSLIGPLLGAISGSMIARGSSFLKD
                     RLGQKIFGDSIQIVDDPLRPRGLGSCLFDDEGVTAERRALIADGVLTTWLLNSSAARQ
                     LGLASTGHASRGLAGAPGVSAHNVVLAAGTRAPQDLMRDAGQGVLITSMFGPSLNSDT
                     GDWSAGASGFAFENGEIAYPVNEITVAGNLIDI"
     gene            1422..>2081
                     /locus_tag="VF402_01885"
     CDS             1422..>2081
                     /locus_tag="VF402_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799069.1"
                     /translation="MFPRWRSAVSKRLSALRDPHADRLRWASEAEEAEAMRPILRQYC
                     LVTGGYYALLIFDRLAHEKGVALVVMLGLTTACMAFGFYAWRLLRDPAPRRRLIALNI
                     GLNVLLYLNPVAYQLLHFDRQRLVYFVFLAFIYAVTGVSLWTVAISVGVTIATLIALA
                     TLNPGVIATNSGAMILVIVPVIVIIFNSIRASMVQAVRARVVAEGLRNEAQKLADCDA
                     LT"
BASE COUNT          319 a          720 c          690 g          352 t
ORIGIN      
        1 agatgtcgat cagattgccc gccacggtga tttcgttgac cggataggcg atctcgccgt
       61 tctcgaacgc gaagcccgag gcgcccgcgg accagtcgcc ggtgtcgctg ttcaaactgg
      121 ggccgaacat cgaggtgatc agcacgccct ggcccgcatc gcgcatcagg tcttgcggcg
      181 cgcgcgtccc ggcggcgagg acgacattgt gcgccgacac gcccggcgcc ccggccagcc
      241 cgcgcgaggc gtggccggtc gaggccagcc ccagttgccg cgcggcggac gagttcagca
      301 gccacgtcgt cagcacgccg tccgcgatca gggcgcggcg ctccgccgtc acgccctcgt
      361 cgtcgaacag gcacgagccg aggccgcgcg gacgtagcgg atcgtcgacg atctggatgg
      421 aatcgccgaa tatcttttgc cccagccggt ctttcaggaa gctggagccg cgcgcgatca
      481 tcgaacccga aatcgcgccg agcagcggcc cgatcagcga gcgcgccacg cgctgatcga
      541 agatcaccgc cgtcttttgc gtgtcgagct tgcgcgcccc cagcgcggcc agcgcgcgtt
      601 cggcgccggt ccgtccgatg tcggcgacgt cgggcaggtc gctcaggaag cgcgtgctgc
      661 ggccttcgcc gtcgcgctcc atctggccgt cgccgccgct ggcgataaag cggcccgact
      721 ggaagaacag ggtgcttcgg tgcgcgccct caaagccgtc cgaggtcaac atcagccagc
      781 ggttctgcgt gtaaccggca ttggccgcgt cgctttgcag ccgcgcgctc agggccagac
      841 ccgccgcctc gatcgccagc gcgcggtcct gaaggctgcg cgcgccgggc tcggccgtat
      901 cgtaaaggtc gagatcgggc gcgtcgtcac tggcgcggta cagccgcgcc ggatcggcca
      961 gccacgcata tgggtcttcg ggcgccagcc gcgccatcgc cacggcgcgc tcgacaagcc
     1021 gcgacagggc ggcgggcgaa aagtccgaca ccgaaaccac cgcctgacgc cgcccgataa
     1081 agacgcgcag gcccagatcg ccggtttcgt cgcgctcgac ggtttcgacc tcgcccttgc
     1141 gcacgccgat gctcagggcg cggctggtcg cgaagaccgc ctcggcggcg tcggcgccgg
     1201 cggccttcgc cgcgcggacc acctcgtgca ggcgcgcctg cgccgtcgaa atctcgtcac
     1261 ttcgcgtttg caacatgaag aatctatagg cccgcccgcc gccggagcgc aagtctcgtt
     1321 tgcgacaatt taactgcgcg aaaatgcgcg gagcttagca gttttttcag ccctcgcggt
     1381 taggtttcac cgtaagagtc cgagcgaagg ttgaaagacg catgttcccg cggtggcgta
     1441 gcgctgtatc caagagactg tcggcccttc gcgatccgca cgcggaccgg ctgcgctggg
     1501 ccagcgaggc cgaggaagcc gaggccatgc ggccgatcct gcgtcagtac tgcctggtca
     1561 cgggcggcta ttacgcgctg ttgattttcg accgtctggc gcacgaaaag ggcgtggcgc
     1621 tggtcgtcat gctgggcctg acgacggcct gcatggcgtt cggtttctac gcctggcggc
     1681 tcttgcgcga tccggcgccg cgccgccgcc tgatcgcgct caatataggg ctgaacgtcc
     1741 ttctgtatct caatccggtc gcctatcaac tgctgcattt cgaccgtcag cgtctggtct
     1801 atttcgtctt tctggccttc atctacgcgg tcaccggcgt cagcctgtgg acggtggcga
     1861 tcagcgtcgg cgtcacgatc gccaccctga tcgcgctggc gacgctgaat ccgggcgtca
     1921 tcgcgaccaa cagcggcgcg atgatcctgg tgatcgttcc ggtcatcgtc atcatcttca
     1981 actcgatccg cgccagcatg gtgcaggcgg tgcgcgcgcg cgtcgtcgcc gagggcctgc
     2041 gcaacgaggc gcagaagctg gccgactgcg acgcgctgac c
//
LOCUS       DASVLL010000114         4303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_275597,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000114 DASVLL010000000
VERSION     DASVLL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275597"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3013)
                     /locus_tag="VF402_01890"
     CDS             complement(<1..3013)
                     /locus_tag="VF402_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX7799070.1"
                     /translation="MSHTLDRLAFFKRKTQTFSQGHGVLNDDDRTWEDAYRDRWRHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRPDLPNHEPRGCARGASYSWY
                     LYSANRVKYPLIRSRLLKLWREARGRLPPAAAWEAIQSDAGKRDSYIRVRGAGGFVRA
                     GWDEVNEIIAAANAYTVRRYGPDRVFGFSPIPAMSMVSYAAGARYLQLLGGVCGSFYD
                     WYCDLPPASPQTWGEQTDVPESADWFNSGFLLLWGSNVPQTRTPDAHFYTEARYKGVK
                     SAVICPDYSEASKFGDIWLAVKQGTDAALGMAFGHVILKEFHQDRHVPYFRDYLRKYS
                     DMPMLVRLVKKGGYFIPERLLRAADFADDLNEANNPDWKTIAVDETTGRIVVPQGSIG
                     FRWGEDGKWNLEEKDASGAETRLRLSLNGVHDDVKGVGFPYFANSASNGFASTDHPDV
                     LVRNIPVKRLALKAGETLVASVYDLFLANYGVDQGFGGDFMPRDFDDPEPYSPAWAEA
                     ITSVPRDKIIAVARGFASNAEKTNGKSMVIIGAAMNHWFHMDMNYRAVINMLVMCGCV
                     GQSGGGWSHYVGQEKLRPQTGWAPIAFATDWAKPPRQQNGTSYFYAHTDQWRYETVSV
                     GELLSPTAPDGAWSGALIDYNVRAERMGWLPSAPALETNSLEIAQAAAAAGLDVRDYV
                     VRSLKDRSLKLSCMDPDKPVNWPRNMFVWRSNLLGSSGKGHEYFLKHLLGATHGVQGK
                     DLGQTGQLKPEEVVWRDEAPEGKLDLLVTLDFRMSTTAVYSDIVLPTATWYEKHDLNT
                     SDMHPFIHPLTAAVDPAWEARSDWAIFKGIAKTFSDIAGEVLGVEQDVVLTPIQHDSA
                     AELAQPFDVRDWYKGECEPIPGRTMSQVTVVERDYPNLYKRFTSLGPLLEKTGNGGKG
                     IGWNTEHEVHALKDLNGRVAEAGVSHGLARIETDIDAAEAIMMLAPETNGEVAVRSWR
                     ALEKQTGRKHDHLALPKEDEKIRFRDIVAQPR"
     gene            complement(3016..>4303)
                     /locus_tag="VF402_01895"
     CDS             complement(3016..>4303)
                     /locus_tag="VF402_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799071.1"
                     /translation="FAVWMVWSMVVAKLKLIGFNFTTDQLFWLTALPALSGATLRVFY
                     SFMVPILGGRLWTVLTTLSLAVPAIGIGLAVQDSNTPYAMFVALAFLCGFGGGNFASS
                     MSNIGFFYPKAQKGNALAVNAGFGNLGVSVMQFLVPLVITSGLFAPVFGAGQLVHDKG
                     VESHLYLQNAGFIWTPFILAAAAAAWFGMNDISTARASFKAQSVVFRRKHNWLMCWLY
                     LGTFGSFIGFSAAFPLLSKTLFPDVLVLKIAFLGPLVGALSRALTGWISDKWGGENVT
                     QVTFAALIAGVLAVLHSVGAFGGTASFPYFFGAFMWLFFWTGVGNASTFQMIPAIVRA
                     SMPRLMPTATAPERLQAAEMESAAIVGFTSAFGAFGGVFIPMAFGQSLKDTGNANTAL
                     FVFLVFYVTCAALNWAVYARKGSLLSRSASTLNPAE"
BASE COUNT          761 a         1383 c         1433 g          726 t
ORIGIN      
        1 tgcgcggctg ggcgacgatg tcgcggaagc ggatcttttc gtcctccttc ggcagggcca
       61 gatggtcgtg cttgcggccc gtctgttttt ccagcgcgcg ccacgacctg accgccacct
      121 cgccattggt ttccggcgcc agcatcatga tcgcctcggc ggcgtcgatg tcggtttcga
      181 tgcgggcgag gccgtgactg acgcccgcct cggccacccg gccgttgagg tctttcagcg
      241 cgtggacctc atgctcggtg ttccatccga tgcccttgcc gccgttgccg gtcttttcca
      301 gcagcggacc gagagaggtg aagcgcttat agaggttggg ataatcgcgc tcgacgaccg
      361 tcacctgcga catcgtcctg cccggtatcg gctcgcactc gcccttgtac cagtccctga
      421 cgtcgaaggg ctgggccagc tcggcggcgc tgtcgtgctg gatgggcgtc agcacgacgt
      481 cctgctccac gcccagcact tcgcccgcta tgtccgaaaa cgtcttcgcg atgcccttga
      541 agatcgccca gtcggagcgc gcctcccacg ccggatcgac ggcggcggtc agcggatgga
      601 tgaacgggtg catgtccgag gtgttcaggt cgtgcttttc gtaccaggtc gccgtcggca
      661 gcacgatgtc ggaatagacc gccgtggtcg acatgcggaa gtcgagcgtg accagaaggt
      721 ccagcttgcc ttccggcgcc tcgtcgcgcc agacgacctc ctccggcttg agctggcccg
      781 tctggcccag atccttgccc tgaacgccgt gcgtggcgcc cagcaggtgc ttcaggaaat
      841 attcgtggcc cttgcccgac gaacccagca ggttcgagcg ccacacgaac atgttgcgcg
      901 gccagttcac cggcttgtcc gggtccatgc acgacagctt cagcgaacgg tccttcagtg
      961 accggacgac gtagtccctg acgtccagtc cggcggcggc ggcggcctgc gcgatctcca
     1021 gcgaattggt ttccagcgcg ggcgcggacg gcagccagcc catgcgctcg gcccggacat
     1081 tgtagtcgat cagggcgccg ctccaggcgc cgtcgggcgc cgtcggcgac agcagttcgc
     1141 ccacggagac ggtttcgtag cgccattggt cggtgtgggc gtagaagtag gaggtgccgt
     1201 tctgctggcg cggcggcttg gcccagtcgg tggcgaaggc gatcggcgcc cagccggtct
     1261 gcggccgcag cttttcctga ccgacatagt gcgaccagcc gccgcccgat tgcccgacgc
     1321 agccgcacat gaccagcatg ttgatgaccg cgcggtaatt catgtccata tggaaccagt
     1381 ggttcatcgc cgcgccgatg atcaccatgg acttgccgtt ggtcttctcg gcgttcgagg
     1441 cgaagccgcg cgccacggcg atgatcttgt cgcgcggcac cgaggtgatg gcttcggccc
     1501 aggccggaga atagggttcg ggatcgtcga agtccctcgg catgaaatcg ccgccgaagc
     1561 cctgatcgac gccgtaattg gcgaggaaga ggtcatagac gctggcgacc agcgtctcgc
     1621 ctgccttgag cgccagccgc ttgaccggta tgttgcgcac cagcacgtcc gggtggtcgg
     1681 tcgaggcgaa accgttgctg gcgctgttgg cgaagtaggg aaagccgacg cctttcacgt
     1741 cgtcatggac gccgttcagg ctgaggcgca ggcgcgtttc cgcgccgctt gcgtcctttt
     1801 cctccagatt ccacttgccg tcctcgcccc agcggaagcc gatcgagcct tgcgggacga
     1861 cgatcctgcc ggtggtttca tcgacggcga tcgtcttcca gtccgggttg ttggcctcgt
     1921 tcaggtcgtc ggcgaagtcg gcggcgcgca gcaggcgttc ggggatgaaa tagccgccct
     1981 tcttcaccag ccggaccagc atcggcatgt cggaatattt gcgcaggtag tcgcggaagt
     2041 acggcacgtg ccggtcctga tggaactcct tcaggatgac gtggccgaac gccatgccga
     2101 gcgcggcgtc cgtgccctgc ttgacggcca gccagatgtc gccgaacttg gacgcctcgg
     2161 aatagtcggg gcagatgacc gccgacttga cgcccttgta gcgcgcctcg gtgtagaagt
     2221 gcgcgtccgg cgtccgggtc tgcggcacgt tggagcccca cagcagcagg aagccggaat
     2281 taaaccagtc cgccgattcc ggcacgtcgg tctgctcgcc ccaggtctgc ggcgacgcgg
     2341 gcggcaggtc gcagtaccag tcgtagaacg acccgcagac gccgccgagc agttgcagat
     2401 aacgcgcgcc cgccgcatag gacaccatcg acatggccgg aatgggcgag aagccgaaca
     2461 cccggtccgg tccgtagcgc ctgaccgtgt aggcgttcgc cgccgcgatg atctcgttga
     2521 cctcgtccca gcccgcgcgc acaaagccgc cggccccgcg cacgcggata tagctgtcgc
     2581 gcttgcccgc gtcgctctgg atcgcttccc acgccgccgc cggcggcagc ctgcccctcg
     2641 cctcccgcca cagcttcagc agccgggagc ggatcagcgg atatttcacg cggttggccg
     2701 aatagaggta ccagctatag ctggcgccgc gggcgcagcc gcgcggctcg tggttgggca
     2761 ggtccggccg ggtgcgcgga tagtcggtct gctgggtttc ccaggtgacg atcccgccct
     2821 tgacgtagat cttccacgag cacgaaccgg tacagttcac gccgtgggtc gaccgcacga
     2881 tcttgtcgtg ccgccagcga tcgcgatagg cgtcctccca ggtgcggtca tcgtcgttca
     2941 gaacgccgtg gccttgcgaa aaggtctggg tcttgcgctt gaaaaaggcg agacggtcaa
     3001 gggtgtggga catggttact cagccggatt taaggttgac gccgacctgc tcagaagcga
     3061 gcccttgcgg gcatagacgg cccagttgag cgcggcgcag gtgacataga agaccagaaa
     3121 gacgaacagg gccgtgttgg cgttgcccgt gtccttcagg gactggccga aggccatggg
     3181 gatgaagact ccgccgaagg cgccgaacgc cgaggtgaag ccgacgatgg ccgcgctttc
     3241 catttccgcg gcctgaaggc gttcgggcgc cgtcgccgtt ggcatcaggc gcggcatcga
     3301 ggcgcggacg atcgccggga tcatctggaa ggtcgaggcg ttgccgacgc cggtccagaa
     3361 gaacagccac atgaaggcgc caaagaagta cgggaaagag gcggtcccgc cgaaggcgcc
     3421 cacgctgtgc agcacggcca gcacgccggc gatcagggcg gcgaaggtga cctgggtgac
     3481 gttctcgccg ccccacttat cggaaatcca gccggtcagc gcgcgcgaca gggcgccgac
     3541 cagcggcccc agaaaggcga tcttcagcac cagcacgtcc gggaacagcg tcttggacag
     3601 cagcggaaag gccgccgaga agccgatgaa cgagccgaag gtgccgagat agagccagca
     3661 catcagccag ttgtgcttcc ggcggaaaac cacggactgc gccttgaagg acgccctggc
     3721 cgtcgagatg tcgttcatgc cgaaccacgc ggcggcggcg gcggccagaa taaacggcgt
     3781 ccagatgaaa ccggcgttct gaaggtacag atggctttcg acgcccttgt cgtgaacgag
     3841 ctggcccgcg ccgaagacgg gcgcgaacag gccgctggtg atcaccagcg ggacgaggaa
     3901 ctgcatcacg ctgacgccga gattgccgaa gcccgcattg acggccagcg cattgccctt
     3961 ctgagcctta ggatagaaga agccgatatt gctcatcgac gaggcgaagt tgccgccgcc
     4021 gaaaccgcac aggaaggcga gcgcgacgaa catcgcatag ggcgtgttcg agtcctgaac
     4081 ggccagcccg atgccgatgg ccggaaccgc cagcgacagc gtcgtcagca ccgtccataa
     4141 acgcccgccg aggatcggca ccatgaagct gtagaaaacg cgcagcgtgg cgccggaaag
     4201 cgcgggcaag gcggtcagcc agaacaactg gtccgtggtg aaattgaagc cgatcagctt
     4261 cagcttcgcc acgaccatgg accagaccat ccagacggcg aag
//
LOCUS       DASVLL010000115         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_59598, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000115 DASVLL010000000
VERSION     DASVLL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59598"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VF402_01900"
     CDS             complement(<1..341)
                     /locus_tag="VF402_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7799072.1"
                     /translation="MRDGRVKEVRPRDGGAKDTPKSRRTRVQILETAMRLFAEVGYDR
                     AGNAAIAEACGLTRGAMLYHFPTREALVEAAAWHIHAAREAAFEAEARKLKPGQDALD
                     GAIDAYWRLLDS"
     gene            685..>1738
                     /locus_tag="VF402_01905"
     CDS             685..>1738
                     /locus_tag="VF402_01905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7799073.1"
                     /translation="MRILLATDAWEPQVNGVVTTLRKTIEECEKLGHEFKIIDYNQFK
                     TVSWPDYPEVKLALGCYEEVREIILDYEPDAIHIATEGFVGMAARRVCLEWKLPFTTS
                     YHTKFPEYISARLPIPLSWGYAFMRWFHKPSGRVMVATPTLQKDLEARGFKNISPWTR
                     GVDTETFRPGLEPIFGDLPRPIMTYIGRVAVEKNLEAFLKLDLPGTKVIVGKGPQLEE
                     LKAKYPDVVFTGPRFGEELARSYADSDVFVFPSLTDTFGLVITEAMAAGTPVAAFPAH
                     GPIDLIPGSGAGAIDEDLSLAIREALKIDRKAVRAYAEKFSWPECAAEFIRNLEPYPE
                     PARTKFWKRLRQLRRKK"
BASE COUNT          323 a          566 c          552 g          297 t
ORIGIN      
        1 gaatcgagca gccgccaata ggcgtcgatg gcgccgtcca gcgcgtcctg accgggtttc
       61 agcttgcggg cctcggcctc gaacgcggcc tcgcgcgcgg cgtggatgtg ccaggcggcc
      121 gcctcgacca gcgcctcgcg cgtcgggaag tggtacagca tggcgccgcg cgtcaggccg
      181 cacgcctcgg cgatggcggc gttgcccgcg cggtcgtagc cgacctcggc gaacagccgc
      241 atcgccgtct ccagaatctg cacgcgggtg cgccgcgact tgggcgtgtc cttggcgccg
      301 ccgtcgcgcg gccggacctc tttcacccgg ccgtcgcgca tcgcggcgtc gcgtttattg
      361 cgcacctgcg gtaaggatgg cgtatctttc ccgtgatcgg tcatctggag ttctttccga
      421 acgcggcggg gaccggacag aacccgcgtt acgtcaaaac atgtttcgct tttaaaaata
      481 agatattttt gctctaaagc aagccgcaac cgcgcctttg cgatcacctc ttgcgacgaa
      541 tttgcaattg tgcaacgcac aaagcgacaa aagcgtcaaa aaaaccggac aaagcccgcg
      601 cccgcggcgg cgcgcattgc cgacttgcca agccgcgccg ctttcggcat ggaagaagca
      661 gggtaaagat taaggatatc cgcattgcga atcttgctgg caacggacgc ctgggagcct
      721 caggtcaacg gcgtggtgac gaccctgcgc aagacgatcg aggagtgcga gaagctcggc
      781 cacgaattca agatcatcga ctacaaccag ttcaagaccg tgtcgtggcc ggactatccc
      841 gaggtcaagc tggcgctggg ctgttacgaa gaagtgcgcg agatcatcct cgactacgag
      901 ccggacgcga tccacatcgc gaccgagggc ttcgtcggca tggcggcgcg gcgcgtgtgc
      961 ctggaatgga agctgccctt caccaccagc tatcacacca agtttcccga atacatctcg
     1021 gcgcggctgc cgatcccctt aagctggggc tacgccttca tgcgctggtt ccacaagccg
     1081 tccgggcgcg tgatggtggc gacgccgacc ttgcagaagg atctggaagc gcgcggcttc
     1141 aagaacatct cgccgtggac gcgcggcgtc gacaccgaga ccttccggcc cggcctggag
     1201 ccgatcttcg gcgacctgcc gcgcccgatc atgacctata tcggccgcgt ggcggtcgag
     1261 aagaatctcg aagccttcct gaaactggac ctgcccggca cgaaggtcat cgtcggcaag
     1321 gggccgcagc ttgaagagct gaaggcgaaa tatcccgacg tggtgttcac cggcccgcgc
     1381 ttcggcgagg agctggcgcg ttcctacgcg gactcggacg tgttcgtgtt tccgtcgctg
     1441 accgacacct tcggcctcgt catcaccgag gcgatggcgg cgggcacgcc ggtcgccgcc
     1501 ttcccggcgc acggcccgat cgacctgatc cccggctccg gcgccggcgc gatcgacgag
     1561 gacttaagcc tcgcgatccg tgaggcgctg aaaatcgacc gcaaggcggt gcgcgcctac
     1621 gccgaaaaat tctcctggcc ggaatgcgcg gcggagttca tccgcaatct ggagccctac
     1681 ccggaaccgg cgcgcaccaa gttctggaaa cgcctgcggc aattgcggcg taagaagg
//
LOCUS       DASVLL010000116         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_59730, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000116 DASVLL010000000
VERSION     DASVLL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59730"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF402_01910"
     CDS             complement(<1..318)
                     /locus_tag="VF402_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799074.1"
                     /translation="MLRRQNWLAPTRAWLGNVHRRPRMLAGLSGLILALAQPPFSFLP
                     GLLGYPLLLWALEQELGPYPKRTAFFMGWLAGFFYFLVSCFWVAEAFLVDAATYGWMA
                     PFAV"
     gene            complement(332..1228)
                     /locus_tag="VF402_01915"
     CDS             complement(332..1228)
                     /locus_tag="VF402_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7799075.1"
                     /translation="MPDPEPPQKKSFFNFLGMGAKPPHGDDRGEAEGTHDGDLISHAR
                     AFQTLTVADVMTPRVDIIGVELGSTLAEVVRLFVESEHSRLPIYRETLDDPVGVIHMK
                     DVLRILSPEADTPIDHWPAFNWNEPVLHRLRREALYVPTSMTTADLLLRMQARRIHMA
                     LVIDEFGGTDGLVTLEDLLEAVVGDIEDEYDEAALEDYRELPNGQVEAAGRMELSDLE
                     ARLGVALYPADAEEEIDTLAGLVAVLAGRVPQRGEVIPYPAAGFDIEVVDADARRIKK
                     LRLHRRGPADNAALPDLTAADD"
     gene            complement(1231..>1579)
                     /gene="ybeY"
                     /locus_tag="VF402_01920"
     CDS             complement(1231..>1579)
                     /gene="ybeY"
                     /locus_tag="VF402_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX7799076.1"
                     /translation="LLCDDAEMRALNRDYRAKDRATNVLSFPAPKSMRAPGMTHLGDI
                     ALGYETCAREAAEQGKSLKNHILHLSVHGALHLLGYDHVRDDEAEEMEALERAILKGL
                     NVPDPYAEDQDAR"
BASE COUNT          260 a          545 c          512 g          262 t
ORIGIN      
        1 gaccgcgaac ggcgccatcc agccataggt ggccgcgtcg actaaaaacg cctcggccac
       61 ccagaagcaa ctgacgagaa aatagaagaa tccggccagc cagcccatga agaaggccgt
      121 gcgcttcgga tacggcccca gctcctgctc cagcgcccag agcagcagcg gatagccgag
      181 caaacccggc agaaaggaga acggcggctg cgccagcgcc aggatcaacc cgctcaggcc
      241 cgccagcatg cgcgggcggc gatggacatt gcccagccat gcgcgcgtcg gcgccagcca
      301 gttttgacgt cgcagcatcg ccggcccgct ttcagtcatc ggcggcggtc aggtccggca
      361 gggcggcgtt gtcggcgggg ccgcgccggt gcaggcgcag cttcttgatg cggcgcgcgt
      421 cggcgtcgac cacctcgata tcgaagcccg ccgccggata ggggatcacc tcgccgcgct
      481 gcggcacgcg cccggccagc acggcgacca gcccggccaa ggtgtcgatc tcctcctcgg
      541 cgtccgcagg gtagagcgcg acgcccagcc tggcctccag atcgctcagc tccatgcgcc
      601 cggcggcctc gacctgaccg ttcggcagtt cgcggtaatc ctccagcgcg gcctcgtcgt
      661 attcgtcctc gatgtcgccg accacggcct ccagcagatc ctccagcgtc accagcccgt
      721 ccgtgccgcc gaactcgtcg atgaccagcg ccatgtggat gcgccgcgcc tgcatgcgca
      781 gcagcaggtc cgccgtcgtc atcgaggtcg gcacatagag ggcttcgcgg cgcaggcggt
      841 gcagcaccgg ctcgttccag ttgaaggcgg gccagtggtc gatcggcgtg tcggcctccg
      901 ggctgaggat gcgcagcacg tccttcatgt ggatcacgcc gaccggatcg tccagcgtct
      961 cgcgatagat gggcaggcgc gaatgttcgc tctcgacgaa cagccgcacg acctcggcca
     1021 gggtcgagcc cagctcgacg ccgatgatgt cgacgcgcgg cgtcatcaca tcggccacgg
     1081 tcagggtctg gaaagcacgg gcgtggctga tcaggtcgcc gtcgtgcgtc ccttccgctt
     1141 cgccccggtc gtcgccgtgc ggcggcttcg cgcccatgcc gaggaagttg aaaaagcttt
     1201 ttttctgcgg cggttccggg tcaggcatga ctatctggcg tcttgatctt cggcgtaagg
     1261 gtcggggacg ttcagcccct tgagaatcgc gcgctccaaa gcctccatct cctcggcctc
     1321 gtcgtcgcgg acatgatcat aacccaataa atgcaaagcg ccatgcacgc tgagatgcag
     1381 aatgtgattt ttcaggctct tgccctgttc ggccgcctcg cgcgcgcagg tttcatagcc
     1441 gagcgcgatg tcgccgagat gcgtcatgcc gggcgcgcgc atcgatttcg gcgccgggaa
     1501 gctcagcacg ttggtcgccc tgtccttggc gcggtagtcc ctgttgagcg cgcgcatctc
     1561 ggcgtcgtcg cacagaagc
//
LOCUS       DASVLL010000117         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_6930, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000117 DASVLL010000000
VERSION     DASVLL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6930"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VF402_01925"
     CDS             complement(<1..863)
                     /locus_tag="VF402_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7799077.1"
                     /translation="MRRVVITGLGIVSSIGTGADEVARSLRDARSGVVFAEDYARLGF
                     KCQVHAAPKIGDWTQLVDRRAARFLAAGTAYGHIAMEQAIASAGLEKADVSNERAGII
                     FGSGGPSTKAIVEAADITREKVSPKRIGPFAVPKAMSSGPSATLATWFEIKGVNYSIS
                     SACATSVHCIGAAAEQIMMGKQDLVFAGGTEELDWTLSDMFDAMGAMSTHFNETPAVA
                     SRAYDRDRDGFVIAGGAGMVVVEDYEHARARGANIIAEIVGYAANSDGYDMVAPSGEG
                     AERCMKIALKEA"
     gene            complement(919..1434)
                     /gene="fabA"
                     /locus_tag="VF402_01930"
     CDS             complement(919..1434)
                     /gene="fabA"
                     /locus_tag="VF402_01930"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEX7799078.1"
                     /translation="MSERPSSFSYEDLLACGRGEMYGPGNAQLPAPPMLMMDRITRID
                     QTGGSHHKGYIEAELDISPDQWFFACHFIGDPVMPGCLGLDAMWQLVGFYLGWLGHPG
                     RGRALGVGEVKFAGQVTPDVRRVAYKIDIKRVMAGKLIMGIGDGVVEADGKPIYQASG
                     LRVGLFDAKSL"
     gene            complement(1574..2146)
                     /locus_tag="VF402_01935"
     CDS             complement(1574..2146)
                     /locus_tag="VF402_01935"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7799079.1"
                     /translation="MQSGGAAIGFALLLLAGSLSLSGCKAHADDAQSYDTPSGLAVPR
                     WAALRAKEVNARNGPSTDNAVLWTYRQPGLPVQIISETRDWRLICDPTGSVAWVSRTL
                     LTNRKQVLTPETQKVQMRAAPNAGAAVKATVEPHALVGLDKCSKSWCRVSVDGVTGWL
                     PQAQLWGAQDAQACQRPGLLTGLGASAAAR"
     gene            2383..3369
                     /locus_tag="VF402_01940"
     CDS             2383..3369
                     /locus_tag="VF402_01940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7799080.1"
                     /translation="MSNAKLKVVLTIKLPDAVETRMRELFNTTLWLDLSPMPRDRLLA
                     AAQDADVLVTSLNDRLDEAFFDAAPERLKMIANFGVGYDHIDIVKAAEKGVIVTNTPG
                     VLTEDTAEMTMGLIMAVSRRFVEGAELVQKGEFSVWSPTFMLGRRIYGKRLGIVGMGR
                     IGQALARRARAFGMSVHYHNRKPVSTRVSDELGATYWDDLDQMLSRMDVVSINCPGGP
                     GTLHLLNAERLSKLQPHAILVNTARGQIVDEKTLAAMLREKRIAGVGLDVYEREPAIN
                     PELIGLPNAILLPHMASSTIEARQDMGDRVILNVKTFQDGHRPPDRVIPAML"
     gene            3456..>4642
                     /locus_tag="VF402_01945"
     CDS             3456..>4642
                     /locus_tag="VF402_01945"
                     /inference="COORDINATES: protein motif:HMM:NF017823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799081.1"
                     /translation="MLRSLSLAALAAFAASAHAETPVTATQGPGTITLDNGLIRAVVD
                     TATGQVTSFRQLGDPPMELIAKGQSLYWDANGEPDAPPAGVDVPKKGYYRPPQHAGLA
                     KLASATPDRADVEVDAAPTPAFPFAVEYHFVMKKGLSGLYAYTALRHPADQPAATLYQ
                     TRFVFRTASDDAIFNYWTVGRGKTIRIPRAGIVSKVTDATWLLADGTVKTKYLNSVYF
                     ARTPVYGTLGVQPSGSHGIWMIEPFGDYHNGGPSRQGQTVHDDVLLRVVQDTHFGSSP
                     VKVAAGEAWSKVYGPFLIYADRAADPNALWDDAERQLAAERAQWPYGFVTASNYVKAR
                     GALNGKVTLDGHVPQNAQAILSDPDPKVAWTAQAKGYNYWSNVDSNGGFALKNIVPGD
                     YTL"
BASE COUNT          817 a         1574 c         1501 g          750 t
ORIGIN      
        1 gcctctttca gcgcgatctt catgcagcgc tcggcgccct cacccgacgg cgcgaccatg
       61 tcgtaaccgt ccgaattggc ggcgtagccg acgatctcgg cgatgatgtt cgcgccgcgc
      121 gccctggcgt gttcgtagtc ttcgaccacc accatgcccg cgccgcccgc gatcacgaag
      181 ccgtcgcggt cgcggtcata ggcgcgtgag gccacggcgg gcgtttcgtt gaagtgggtc
      241 gacatggcgc ccatcgcgtc gaacatgtcc gacagcgtcc agtccagctc ctcggtgccg
      301 cccgcgaaaa ccagatcctg cttgcccatc atgatctgct ccgccgccgc gccgatgcaa
      361 tgcaccgagg tcgcgcaggc cgaggagatc gaatagttga cgcccttgat ctcgaaccag
      421 gtggccaggg tcgcggacgg gccggacgac atggccttgg gcaccgcgaa cggcccgatg
      481 cgcttggggg agaccttttc gcgcgtgatg tcggcggctt cgacgatggc cttggtggac
      541 gggccgcccg aaccgaagat gatgccggcg cgctcgttcg agacatcggc tttctccagc
      601 cccgccgagg cgatcgcctg ctccatcgcg atgtggccgt aggccgtgcc cgcggccagg
      661 aagcgcgcgg cgcggcggtc gacaagctgc gtccagtcgc cgatcttcgg cgcggcgtgc
      721 acctggcact tgaagcccag acgcgcatag tcctcggcaa agacgacgcc agaacgcgca
      781 tcccgcaacg accgggcgac ttcgtcggcg cccgtcccga tggaagatac gatccccaag
      841 cccgtgatga cgactctacg catataaacg tcctaattca gttccctgtg atgatcccgc
      901 gccgtcttga acagcggctt ataaactctt ggcgtcgaac aggccgacgc gcaggcccga
      961 cgcctgataa atgggtttgc cgtcggcttc aacaaccccg tcgccgatgc ccatgatcaa
     1021 tttcccggcc atgacgcgct tgatgtcgat cttgtaggcg acgcgcctga cgtccggcgt
     1081 gacctgaccg gcgaacttga cctcgccgac gcccagcgcg cggccgcgcc ccggatggcc
     1141 cagccagccg aggtagaagc cgaccagttg ccacatggcg tcgaggccga ggcagcccgg
     1201 catgaccgga tcgccgatga agtgacaggc gaagaaccac tggtccggcg agatgtcgag
     1261 ttcggcttcg atatagccct tgtggtggct gccgcccgtc tggtcgatgc gggtgatgcg
     1321 gtccatcatc agcatcggcg gcgccggcag ttgcgcgttg ccggggccgt acatctcgcc
     1381 ccggccgcag gccagcaggt cttcgtagga gaaggaggaa ggacgttcgc tcatgcgctt
     1441 tatcaaggct ccacggcatc ccggcggcgg cgcgtcgtcg atgcgtcata cgtaaaatca
     1501 aggaaggatg cgttacacga ggcgggcctt cggctcaacc ctttataggt gaaatcgcaa
     1561 cgataagcgg ctcttaccgc gccgcggccg aggcgccgag cccggtcagc aggccgggcc
     1621 gctggcaggc ctgcgcgtcc tgcgcgcccc agagctgggc ctgcggcagc cagccggtga
     1681 cgccgtcgac cgagacgcgg caccagcttt tgctgcattt gtcgaggccg accagggcgt
     1741 gcggctcgac cgtcgccttg acggcggcgc ccgcattggg cgcggcgcgc atctggacct
     1801 tctgcgtttc gggcgtcagc acctgcttgc gattggtcag aagcgtgcgg ctgacccagg
     1861 cgacgctgcc cgtgggatcg cagatcagcc gccagtcgcg cgtctcggaa atgatctgca
     1921 ccggcaggcc gggctgccgg taggtccaca gcaccgcgtt gtcggtgctg gggccgttgc
     1981 gggcgttgac ctccttggcg cgcagcgcgg cccagcgcgg cacggccagc ccggagggcg
     2041 tgtcgtagct ctgcgcgtcg tcggcgtgcg ccttgcagcc gctcaaagac agcgaacccg
     2101 ccagcagcag caacgcaaag ccgatcgccg cgcccccgct ttgcacttga aaacgcgcgc
     2161 ccaaaccgct tatgtgcgcg gaggaagcct tatctggaaa ctcgctcagg tcggacatga
     2221 ccgcagaaaa ctatgccggt gaaatgaaaa gggcgcgcat gagcagccgc gcccaagcat
     2281 cggcgtcaag cgttcagatt cggttaacag aagactaatt tttcagcgcg cggacggcga
     2341 agggaaagcg ccgtccgcgc gcccgcacaa caaaaggcgc gcatgagcaa cgctaaactc
     2401 aaggtcgtcc tgaccatcaa actgcccgac gcggtcgaga cgcggatgcg cgaactgttc
     2461 aacaccacgc tgtggctcga cctgtcgccc atgccgcgcg acaggctgct ggcggcggcg
     2521 caggacgcgg acgtgctggt cacctcgctc aacgaccggc tggacgaggc gtttttcgac
     2581 gccgcgccgg agcggctgaa gatgatcgcc aatttcggcg tcggctacga ccatatcgac
     2641 atcgtcaagg cggcggaaaa gggcgtgatc gtcaccaaca cgcccggcgt gctgaccgag
     2701 gacacggcgg aaatgaccat gggcctgatc atggccgtgt cgcgccgctt cgtcgagggc
     2761 gccgagctgg tgcagaaggg cgaattctcc gtctggtcgc cgaccttcat gctggggcgg
     2821 cgcatctacg gcaagcggct cggcatcgtc ggcatgggcc gcatcggtca ggcgctggcc
     2881 cggcgcgcgc gcgccttcgg catgagcgtg cattatcaca accgcaaacc ggtctcgacg
     2941 cgcgtgtccg acgaactggg cgcgacctac tgggacgatc tcgatcagat gctgtcgcgc
     3001 atggacgtgg tgtcgatcaa ctgccccggc ggccccggca cgctgcatct gcttaacgcc
     3061 gagcgcctgt cgaagctgca accgcacgcc atcctcgtca atacggcgcg cggccagatc
     3121 gtcgacgaaa agaccctggc cgccatgctg cgcgaaaagc gcatcgcggg cgtcggcctc
     3181 gacgtctatg agcgcgaacc ggcgatcaat cccgaactga tcggcctgcc caacgcgatc
     3241 ctcctgccgc acatggcctc gtccaccatc gaggcgcggc aggacatggg cgaccgcgtc
     3301 atcctcaacg tcaagacctt tcaggacggc caccgcccgc cggatcgcgt catcccggcg
     3361 atgctgtagg gcgtcgcggc gacggaatct gattgccgga gcgtataatt gcgcataatc
     3421 gatcatttat gcgcaatcgt tcagggatcg ccgtcatgct ccgtagttta agcctcgccg
     3481 cgctcgcggc cttcgccgcg tccgctcacg ccgaaacgcc ggtgacggcg acgcaaggcc
     3541 ccggaacgat cacgctcgac aacggcctga tccgcgccgt ggtcgatacg gccaccggtc
     3601 aggtgacttc gttccgacaa ctgggcgatc ctccgatgga gctgatcgcc aaaggccagt
     3661 cgctgtactg ggacgccaac ggcgagccgg acgcgccgcc cgccggggtc gacgtgccga
     3721 aaaagggcta ttaccgcccg ccgcagcacg ccggtctggc gaagctggcc tcggccacgc
     3781 cggatcgggc cgatgtcgag gtcgacgccg cgccgacccc cgccttcccg ttcgcggtcg
     3841 agtaccattt cgtcatgaag aagggcctgt ccgggcttta cgcctatacg gcgctgcgcc
     3901 atccggcgga ccagcccgcc gcgaccctgt accagacgcg cttcgtgttc cgcaccgcct
     3961 cggacgacgc gatcttcaac tactggacgg tcgggcgcgg caagacgatc cgcatcccgc
     4021 gcgccgggat tgtcagcaag gtcaccgacg cgacctggct gctggcggac ggcacggtca
     4081 agaccaagta cctgaactcg gtctatttcg ccaggacgcc ggtgtacggc acgctgggcg
     4141 tgcagccgtc cggcagccac ggaatctgga tgatcgagcc gttcggcgat tatcacaacg
     4201 gcggccccag ccgtcagggc cagaccgtgc atgacgacgt gctgctgcgc gtggtgcagg
     4261 acacgcattt cggctcgtcc ccggtcaagg tcgccgcggg cgaggcgtgg agcaaggtct
     4321 atggcccgtt cctgatctac gccgaccgcg ccgccgatcc gaacgcgctc tgggacgacg
     4381 ccgagcgcca actggccgcc gagcgcgcgc aatggcccta cggcttcgtc accgccagca
     4441 attacgtcaa ggcgcgcggc gcgttgaacg gcaaggtcac gctggacggc catgtcccgc
     4501 aaaacgcgca ggccatcctg tccgatcccg acccgaaggt ggcgtggacc gcgcaggcga
     4561 agggttataa ctattggtcc aacgtcgatt ccaacggcgg cttcgccttg aaaaatatcg
     4621 ttccgggcga ttacaccctc ta
//
LOCUS       DASVLL010000118         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_6969, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000118 DASVLL010000000
VERSION     DASVLL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6969"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VF402_01950"
     CDS             complement(<1..1827)
                     /locus_tag="VF402_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799082.1"
                     /translation="MSAGAPWSVKGIDAKAREVAKDLARRSGMTLGEWLNQMILEGRD
                     VGALIHDERSRAEAGRARRVAEAAYEDDTYELDAPAPSRSRPASRHTMPPAWADRPRA
                     PYSTGERPLSSRDLRRRSIFEDAPRETEDLTRVARAIESLGTRIETSESRSAGAVRSV
                     SAAVESLLNRLERSEAAHAETRETLEEHTVAAAQRLSRAEADSGLFVERLDQAERLID
                     AQAERLEGLSGHLREERERVARLEAHLKDGPVMDNVRSLEGALGKLANQIYEGEARAR
                     DTLKDVREDMLGLSHRVAQMEMRDPERVAQGLIDKVVAQLSQRLEAAEAQTTGAVRNL
                     EHAFTQLESRMSRAEERGDVTDPEAVRSLKGLADDLSRQVAESRREWRQALDDNRQQT
                     LDQAVRAVETRLHASERRSASALEKMGQDVLRVADNLNRRMTGVETSAQDGLNRMGRE
                     MQRVAEGLDKRMARSETGHAQALERLGGEIARISERLSAKLADSERRTAEVLTSVGEQ
                     VDHQRDHVRDDLAERIRQSEARTAKLLEETRARIDEKLARVQTESLLSEAARAPGAGA
                     GSDDDGDLPSPFAAAGFARPPVAPALAAVEASEPAEPAASPVT"
     gene            2040..2261
                     /locus_tag="VF402_01955"
     CDS             2040..2261
                     /locus_tag="VF402_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799083.1"
                     /translation="MDLTTTLTVILFAVSSLLTVFFGFMGARPMDPRKGPRMVPWRFL
                     MLLSFTAALLLLVHLLTALGLKSDPPMRY"
BASE COUNT          334 a          824 c          802 g          481 t
ORIGIN      
        1 ggttacgggg gacgctgcgg gttcggcggg ttcggatgct tcgacggccg ccagcgccgg
       61 cgcgactggc gggcgggcga aaccggccgc cgcgaacggg ctgggcaggt cgccgtcgtc
      121 atcgcttccg gcgcccgcgc ccggcgcgcg cgcggcttcg gacagcaggc tttcggtctg
      181 gacgcgcgcc agcttctcgt cgatgcgcgc gcgcgtctcc tccagcagct tcgcggtgcg
      241 cgcctcgctc tggcggatgc gttcggccag atcgtcgcgc acatggtcgc gctgatggtc
      301 gacctgctcg ccgacgctgg tcagcacctc ggcggtgcgg cgttcgctgt cggccagctt
      361 ggcgctgagg cgctcggaaa tccgcgcgat ctcgccgccc agacgctcca gcgcctgcgc
      421 gtggccggtt tcgctgcgcg ccatgcgctt gtccagccct tcggcgacgc gctgcatctc
      481 gcggcccatg cggttgaggc cgtcctgcgc ggacgtctcg acgccggtca tgcggcggtt
      541 gagattgtcg gcgacgcgca gcacgtcctg ccccatcttc tccagcgccg aggccgagcg
      601 ccgctccgac gcgtgcaggc gcgtctcgac cgcgcgcacc gcctgatcca gcgtctgctg
      661 gcggttgtcg tccagcgcct gccgccattc gcggcgcgat tcggccacct gacgcgacag
      721 gtcgtcggcg aggcccttca gcgagcgcac ggcttccggg tcggtgacgt cgccgcgctc
      781 ctcggcgcgg ctcatgcggc tttccagttg cgtgaaggcg tgctccagat tgcgcaccgc
      841 gccggtggtc tgggcctcgg cggcctccag ccgctgcgac aactgcgcca cgaccttgtc
      901 gatcaggccc tgcgcgacgc gctcggggtc gcgcatctcc atctgcgcca cccggtgcga
      961 caggcccagc atatcctcgc gcacatcctt cagcgtgtcg cgcgcgcgcg cctcgccttc
     1021 gtagatctgg ttggccagct tgcccagcgc gccttcgagc gagcggacat tgtccatgac
     1081 cggaccgtcc ttgagatgcg cctccagccg cgcgacgcgc tcgcgctcct cgcgcagatg
     1141 gccggacagg ccctccagcc gctcggcctg cgcgtcgatc aggcgttcgg cctgatcgag
     1201 gcgttcgacg aacaggccgc tgtcggcctc ggcgcggctc aggcgctggg cggcggccac
     1261 ggtgtgctcc tccagcgtct cgcgcgtttc ggcgtgggcg gcttcggagc gctccagccg
     1321 gttcagcagc gattcgaccg ccgccgacac gctgcgcacc gcgcccgccg accggctttc
     1381 ggaggtttcg atgcgcgtcc ccagcgattc gatcgcgcgc gcgacgcggg tcaggtcttc
     1441 ggtttcgcgc ggcgcgtcct cgaagatcga gcgacggcgc aggtcgcgcg agctgagcgg
     1501 gcgctcgccg gtggaatagg gcgcgcgcgg acggtcggcc caggccgggg gcatggtatg
     1561 gcgtgaggcg ggtcgcgaac gggacggcgc gggcgcgtcc agttcgtagg tgtcgtcttc
     1621 gtaagccgcc tcggccacgc ggcgcgcgcg tccggcctcg gcgcggctgc gctcgtcgtg
     1681 aatcagcgcg ccgacgtcgc ggccttcgag aatcatctgg ttgagccatt cgcccaaggt
     1741 catgccggaa cggcgcgcca gatccttggc gacttcccgc gccttggcgt cgatgccttt
     1801 aacactccat ggtgcgcccg cgctcatgcg attcccctag caaggtttgg gtcagcctag
     1861 accgcatcat ctagcgtgta aacactccgt taaccacaat atcttgccta tggttaatct
     1921 tctgtggaaa ttcgcggcgc atgactcgga tgaaaaagga gccgcttttg cggtcggcgg
     1981 cgccgcgttt ccggcgctga cggcttgcgg tccggcgcat tccgcgacat aggcgaagca
     2041 tggacctcac aaccacgctt acggtgattc tgttcgccgt gtcatccctg ctgacggtgt
     2101 ttttcggttt catgggcgcc cgcccgatgg acccgcgcaa aggaccgcgc atggtcccgt
     2161 ggcgctttct gatgttgctg agtttcacgg cggccttgct gctgcttgtg caccttctga
     2221 ccgcgctggg cctgaaatcc gacccgccca tgcggtatta ggcttaaact caggccgcat
     2281 cgccgcgtat ataagtgaat aataagacat tagaagtctt ttttattgta ttatgtgaat
     2341 ataaactttt gaatatataa caatcgttga atatatttat aatttttttc ttcagtctac
     2401 atctttaaaa acaacaaaat tacatcgtga tcggtagggt a
//
LOCUS       DASVLL010000119         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_168526,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000119 DASVLL010000000
VERSION     DASVLL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168526"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VF402_01960"
     CDS             complement(<1..882)
                     /locus_tag="VF402_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799084.1"
                     /translation="MRARRNNREYRSLANITESASTSRILNLAQVAAASAGDPDYARR
                     PFFQNGKLNKAILIKHTLRPNELELFARPRRSATKIILPFESQDLRLGGEFLFVNQIG
                     FERQMRAFTDVMEATGQKDLEVLRIIDSLPSLDPFLVRESLGRQGFAPAPCYLKISNS
                     DIEAMIGFANAEIERLIAIAFGGSASGAAMRFAGKILADRLDNELEPLKQTLRLSDDQ
                     FRDGIFSWRGFLYFKWRRLELRDQLRNVADSLMKYRPVGVMDQALRDYIEEVRPRLIE
                     RVLTAMDMVGRSLLYYDD"
     gene            complement(888..2045)
                     /locus_tag="VF402_01965"
     CDS             complement(888..2045)
                     /locus_tag="VF402_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799085.1"
                     /translation="MNAALQIRTSPTAAPRPVKMSRDLRNLANLGETASTSRVLNLSQ
                     IHLVSGDEPEYRARPFFQDSRLNKAIIIKHTLRANEQDAFHRPRRTATKVVLPFDPAD
                     LKLGASSVFVGQIGFDRFCRDFLGLDDINGDNDAQILRLLDSIPSLDPFLVRELLGRH
                     GFKPAPCYLKIANSDIQRMIGFANSEIEKLVRIAFGTTIYGASIKLASKILSNELDNE
                     LLPLKATLRLSDEAFSDGIFSWRGFLYFKWRHLELQEEMNRVIDGVARYQPRGKAEPD
                     VLGYLEETRPRLARRILSAIAAIGRTLLIYDRAYAALVEQGDPTPFRQFLLDGPSLFY
                     ELGESVAILGHISSFWNYRLNQNDYYDKLDSAEYADILMDFDDSLSSLSDV"
BASE COUNT          385 a          659 c          759 g          399 t
ORIGIN      
        1 gtcgtcatag tacagaaggc tgcgtcccac catgtccatc gcggtcagca cgcgctcgat
       61 caggcgcggg cgcacctcct cgatatagtc gcgcagggcc tgatccatca cgccgacggg
      121 ccggtatttc atcaggctgt cggccacgtt gcgcaactgg tcgcgcagtt ccagccgccg
      181 ccatttgaag tacaggaagc cgcgccacga aaagatgccg tcgcggaact ggtcgtcgct
      241 gagccgcagg gtctgtttca gcggctcaag ctcgttgtcc agccggtcgg ccaggatctt
      301 gcccgcgaag cgcatggccg cgccggaggc ggagccgccg aacgcgatgg cgatcagccg
      361 ctcgatctcg gcgttggcga agccgatcat cgcctcgatg tcgctgttgg agatcttcag
      421 atagcacggc gccggggcga agccttgccg ccccagcgat tcgcgcacga ggaacgggtc
      481 cagcgagggc agggagtcga tgatgcgcag gacttcgagg tctttctggc ccgtcgcctc
      541 catgacgtcg gtgaaggcgc gcatctggcg ctcgaagccg atctggttga cgaacaggaa
      601 ctcgccgccc agccgcagat cctgactctc gaacggcagg atgatcttgg tcgccgagcg
      661 gcgcgggcgc gcgaacagct ccaactcgtt cgggcgcagg gtgtgcttga tcaggatcgc
      721 cttgttcagc ttgccgttct ggaagaaggg ccggcgcgca tagtccgggt cgcccgccga
      781 ggccgccgcg acctgcgcca gattcaggat gcggctggtc gaggccgatt cggtgatgtt
      841 ggcgaggctg cggtattcgc ggttgttccg ccgggcgcgc atggcggtta tacgtcggac
      901 aggctggaca ggctgtcgtc gaaatccatc aggatgtcgg cgtattcggc ggagtccagc
      961 ttgtcgtaat agtcgttctg gttcaggcgg taattccaga acgagctgat atggccgagg
     1021 atggcgacgc tttcgcccag ttcgtagaac aggctggggc cgtccagcag gaactggcgg
     1081 aacggcgtcg ggtcgccctg ttcgaccagc gcggcgtagg cgcggtcgta gatcagcagg
     1141 gtgcgcccga tggcggcgat cgcgctcagg atgcggcggg ccagacgcgg gcgcgtctcc
     1201 tcaaggtagc ccagaacgtc cggctcggcc ttgccgcgcg gctgataccg ggccacgccg
     1261 tcgatgacgc ggttcatctc ctcctgcaat tccagatgcc gccacttgaa atagaggaag
     1321 ccgcgccacg agaagatgcc gtcggaaaac gcctcgtccg acaggcgcaa ggtcgccttg
     1381 agcggcagaa gctcgttgtc cagctcgttg gacaggattt tcgacgccag cttgatcgag
     1441 gcgccgtaga tggtggtccc gaaggcgatg cgcaccagct tctcgatctc gctgttggcg
     1501 aagccgatca tgcgctggat gtcgctgttg gcgatcttca ggtagcacgg cgcgggcttg
     1561 aaaccgtgcc gccccaaaag ctcgcgcacc aaaaacgggt ccagcgacgg gatcgaatcg
     1621 agcaggcgca gaatctgcgc gtcgttgtcg ccgttgatgt cgtcgaggcc gaggaagtcg
     1681 cggcagaacc ggtcgaagcc gatctggccg acgaagaccg aactggcgcc cagtttcagg
     1741 tccgccgggt cgaacggcag cacgaccttg gtggcggtgc ggcgcgggcg gtgaaaggcg
     1801 tcctgttcgt tggcgcgcag ggtgtgcttg atgatgatcg ccttgttcag gcgcgaatcc
     1861 tgaaagaagg gccgggcgcg gtattccggc tcgtcgcccg aaaccagatg aatctgcgac
     1921 aggttcagca cgcgcgaggt cgaggccgtc tcgcccagat tggccaggtt gcgcaggtcg
     1981 cgcgacatct tgaccgggcg cggcgccgcc gtcgggctgg ttctgatctg gagcgctgcg
     2041 ttcatgcggc ggggcccttg aagcgggtcg tgaagaaaag ggtcgaaccc gtccggccgc
     2101 ctagggcgcg gaaaggctct taacctgagg ttgtttatgc agagccaaga gtaaataatc
     2161 cctctccgcc gcccgccggt aacggttatt tttcaccatg aa
//
LOCUS       DASVLL010000120         7010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_7129, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000120 DASVLL010000000
VERSION     DASVLL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7129"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..913
                     /gene="rimM"
                     /locus_tag="VF402_01970"
     CDS             416..913
                     /gene="rimM"
                     /locus_tag="VF402_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271087.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7799086.1"
                     /translation="MKPHLVLVAQLGAPHGVQGEIKLLSFTDDPLSVLEYNPLLDDRG
                     EAALSVTAAREHKGALLIRAEEIADRTAAEKAKGLKLYIRREDLPEPDTDEYYVADLI
                     GMDVVDVQGQPVGKVANVDNFGAGDLLDIRPLAGANFYVAFTGDHVPEVKLSERVIVV
                     DLSGV"
     gene            998..2017
                     /locus_tag="VF402_01975"
     CDS             998..2017
                     /locus_tag="VF402_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7799087.1"
                     /translation="MRRPTSFDIAALAGVSQATVSRALSGAPSVSDAIRRRVYEAAAQ
                     LNYTVDINARKLRSKKINTIAVLVSQDLDQPDNRINPFFLPLIGSILDYASDKGVDVL
                     VSLQKDGDDWSADYGFARRADGIIFLGYRNFAAYRQKVESLRAIGEPWVVFGPEMPET
                     PDLFVGSDNEGGAFEAVRHLIGLGRRRIVFLGEASEAHSEFFERYKGYVRAHAAADLR
                     VDPALAIDCFISREEGAAAIDRLISEGAAFDAVFAVTDLLAVGAIQSLQKHGRHVPKD
                     VSVMGFDDQWVCACTWPPLSSVHQDTATAARVLVDTLASLIDNEPVSMTRIPTRLVIR
                     ESCGG"
     gene            complement(2116..4581)
                     /locus_tag="VF402_01980"
     CDS             complement(2116..4581)
                     /locus_tag="VF402_01980"
                     /EC_number="3.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004339 - glucan 1,4-alpha-glucosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009251 - glucan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan 1,4-alpha-glucosidase"
                     /protein_id="HEX7799088.1"
                     /translation="MLTALSGAGARAQDEAVTPENHNPTLNADAPASNGPGLASPWSY
                     SGKQGIGTAYEAYDKDGQFSDKAVTGPVSKVWFSLAQGVLTETMYGRIHEAQIREMQI
                     AVTGHDASGGWTAFEDADTISRVAYIDTDAAGRPLAPAYRLTNTDKQGRFVIIKDVFT
                     DPDRQSVMVRVTVKALKGPVTPYVILDPSMANTSGDDEGAASASALNAWQNNGGQDKV
                     ALSLKASAPFAAHTVGFTGVTDGVADLTQRQTLIAQHDSTGGVKGNIVLIGRLPTVTK
                     AATYDLALGFGADVKAADAEAAGTLKTGYAKVLANFEGRKTRNGGAAVGWEDYLASLS
                     ALKSLIPESEDDGKLLYASALMLKTQEDHTSAGALIASLSNPWGDELKTDVSSTGYKA
                     VWPRDFYQVAMALAALGDRETPVAAYRYLPTVQVRPDTPGNTGVSGWFQQKSHVDGTP
                     EWVGVQLDQTAMPILLGARLEKLGLISHGELMSSYRTMLKPAADFLSKGGQVGLGWNH
                     AVIRPPYSQQERWEEQEGYSPSTTAAVIAGLTAAAGIAAEAGDKDGAALYQAKADDDS
                     AKLEARLFTTDGKVMGEDRPASYYLRISRSEDANNPGVQDERNGHPGLRSDLMIDAGF
                     LELVRYGVRPADDPRIEDSLKVIDDQDLPENLRVRYDFTFPGSDVVIPGFRRYGNDGY
                     GDKTSGGDYAVSGQMSPDQRGRVWPIFTGERGHYQLALDALKPGGATQADIDAIRNIY
                     VRGMEDFANPGLMIPEQVWDGVGSPDPHGYVKGQGTDSATPLAWSHAEYVKLLRSLRD
                     KAVWDRNDATFQRYGRHSASVKD"
     gene            5357..6376
                     /locus_tag="VF402_01985"
     CDS             5357..6376
                     /locus_tag="VF402_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7799089.1"
                     /translation="MDKPSGKIRLEDLAKIAGVSVATVSRALNDSPAVNRVTKRRVWK
                     IARDHNYVLNQDMPLSMSGSQATIAIVIPPPHGREGRFSDPFYQEMISGVGEAARDMG
                     CDLLLSHLTPRSQNDLVALMQNIKADGVIFLGQSYLHDRFNHLVETTRNFIVWGQELP
                     GQKYCTVGSNNLQGGARATAHLARLGRRRIAYFGETEGSEMKLRFQGYLNGLEEAGVE
                     FDPRLVVPTHFELASAEAAVLNLIHRGVAFDGLFACSDIIAFGAIRGLMRAGRDVPRD
                     VSVVGYDNIQLASLTRPALTTISQDMARAGKLMVSKLIGSISNDESHSEIVSTELVVR
                     ESCGA"
     gene            6732..>7010
                     /locus_tag="VF402_01990"
     CDS             6732..>7010
                     /locus_tag="VF402_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799090.1"
                     /translation="MHISKGRKAAYRSRLLTCGATLAIVAACAATGAQAQDANAAKTQ
                     TQASGDTTTVVVTGIRKGIQDAITAKKKSAQIVEAVSAEDIGKLPDQSI"
BASE COUNT         1208 a         2262 c         2298 g         1242 t
ORIGIN      
        1 caaggccgag gcggaagccg ccgccgccgc gcccgccgct gaggaagccc cggttgagga
       61 agcccggccg aggaagccgc cgcttcggaa taagcggcgt tacgacgcct tgacgttcag
      121 ggccggggtg caatcccggc cctttgttgt aagccccttt gtagagtcat atccggcagg
      181 agggggccat gaaacttcga cttatgatta cgatcgtctc tgtggtgctg gcggcgggtc
      241 tggcggcctg cgcgaacctg gccttagcgc cgttgagttc gcccatgccc acgggaccgg
      301 agccgtggca tagggcggga tgctccgcgt cttcgagcgt cagcatgtcg cgcggacaat
      361 atcccagcac gccgaccccg atgcagaagg acgcgctttg cgaatgagcc aagacatgaa
      421 accccatctg gttctggtcg cgcaactggg cgcgccgcat ggcgtgcagg gcgagatcaa
      481 gctgctgagc tttaccgacg atccgctgag cgtgctggaa tataacccgc tgctcgacga
      541 caggggcgag gccgcgctga gcgtcaccgc cgcgcgcgag cataagggcg cgcttctgat
      601 ccgcgccgag gagatcgccg accgcacggc ggcggaaaag gcgaagggcc tgaagctcta
      661 tatccggcgc gaggatctgc ccgaaccgga cacggacgaa tattacgtcg ccgacctgat
      721 cggcatggac gtggtcgatg tgcagggcca gccggtcggc aaggtcgcca atgtcgataa
      781 tttcggcgcc ggcgatctgc tcgacatccg cccgttggcg ggcgcgaact tctatgtcgc
      841 cttcaccggc gaccatgtgc cggaggtgaa gctgtccgag cgcgtgatcg tggtggacct
      901 gagcggcgtt taaggcgcgc cgccgccgga cgcgttatgc cgccttgcct aagcgggcgg
      961 gcaatgccat gctgcgcggc aaaccgataa aaagcagatg agacgcccca cctctttcga
     1021 catcgccgcc ctcgccgggg tcagtcaggc cacggtgtcg cgcgcccttt ccggggcgcc
     1081 gtcggtcagc gacgccatcc gccggcgcgt ctatgaggcg gcggcccagc tcaactacac
     1141 ggtcgacatc aacgcgcgca aactgcgctc gaagaagatc aacaccatcg ccgtgctggt
     1201 gtcgcaggat ctcgaccagc ccgacaaccg catcaatccg ttcttcctgc cgctgatcgg
     1261 ctcgatcctc gactacgcct cggacaaggg ggtcgacgtg ctggtctcgc tgcaaaagga
     1321 cggcgacgac tggagcgccg attacggttt cgcgcgccgc gccgacggca tcatcttcct
     1381 cggctaccgc aatttcgcgg cctaccgtca gaaggtcgaa tccctgcgcg ccatcggcga
     1441 accgtgggtg gtgttcgggc cggaaatgcc ggagacgccg gacctgttcg tcggctccga
     1501 caacgagggc ggcgcgttcg aggccgtccg gcatctgatc gggctggggc ggcggcgcat
     1561 cgtgtttctg ggcgaggcgt ccgaggcgca ttcggagttc ttcgagcgct ataagggcta
     1621 tgtccgcgct catgcggcgg cggatttgcg cgtcgatccg gcgctggcca tcgactgctt
     1681 catctcgcgc gaggaaggcg cggcggcgat cgaccgcctg atctccgaag gcgcggcgtt
     1741 cgacgccgtc ttcgcggtca ccgacctgtt ggccgtcggc gcgatccagt ccctgcaaaa
     1801 acacgggcgg catgtgccga aggacgtctc ggtcatgggc ttcgacgatc agtgggtctg
     1861 cgcctgcacc tggccgccct tgtcgagcgt gcatcaggac acggccaccg ccgcgcgcgt
     1921 gctggtcgac acgctggcca gtctgatcga caacgaaccg gtgtcgatga cgcgcatccc
     1981 gacgcggctg gtcatccggg aatcgtgcgg aggatagcgc ccggcgtcct gacgggcgtt
     2041 gcatgtcttt cgcggccaat aaaaaagccc ccggaacatc cgtccggggg cagttggggt
     2101 caaaggggtt gagacttaat ctttgaccga agcggaatgg cgtccatagc gctggaaggt
     2161 cgcgtcgttg cggtcccaga ccgccttgtc gcgcaggctc cgcagcagct tgacatattc
     2221 ggcgtggctc caggccagcg gcgtggccga gtcggtgccc tggcccttga cgtagccgtg
     2281 cggatcggga gagccgacgc cgtcccagac ctgttccggg atcatcaggc ccggattggc
     2341 gaaatcctcc atgccgcgca catagatgtt gcggatggcg tcgatatcgg cctgcgtggc
     2401 gccgccgggc ttgagcgcgt ccagcgccaa ctggtaatgg ccgcgttcgc cggtgaagat
     2461 cggccagacg cggccgcgct ggtccgggct catctggccg ctaaccgcat agtcgccgcc
     2521 cgacgtcttg tcgccatagc cgtcgttgcc gtagcggcgg aagccgggaa tgacgacgtc
     2581 cgagccgggg aaggtgaagt cgtagcgcac gcgcaggttt tccggcagat cctgatcgtc
     2641 gatgaccttc agcgagtcct cgatgcgggg atcgtcggcc gggcgcacgc catagcgcac
     2701 cagttcgagg aagcccgcat cgatcatcag atcggagcgc agccccggat ggccgttgcg
     2761 ttcgtcctgc acgccggggt tattggcgtc ctcgctgcga gaaatgcgca gataatagga
     2821 tgcgggcctg tcctcgccca tcaccttgcc gtcggtggtg aacaggcgcg cctccagctt
     2881 cgccgaatcg tcgtcggcct tggcctgata gagcgcggcg ccgtccttgt cgcccgcctc
     2941 tgcggcgatg cccgccgcgg cggtcaggcc cgcgatcacg gcggcggtcg tcgagggcga
     3001 atagccttcc tgctcctccc agcgctcctg ctgcgaatag ggcggcctga tcacggcgtg
     3061 gttccagccg aggccgacct gaccgccctt cgacaggaag tcggcggcgg gcttgagcat
     3121 cgtccggtac gacgacatca gctcgccgtg cgagatcagc cccagctttt ccaggcgcgc
     3181 gcccagcagg atcggcatgg cggtctggtc gagctgcacc ccgacccatt cgggcgttcc
     3241 gtcgacatgc gacttctgct ggaaccagcc ggagacgccg gtattgccgg gcgtgtcggg
     3301 cctgacctgc acggtcggca gatagcgata ggcggcgacc ggcgtttccc ggtcgcccag
     3361 cgcggccagc gccatcgcga cctgatagaa gtcgcgcggc cacaccgcct tatagccggt
     3421 cgaggacacg tcggtcttga gttcgtcgcc ccacgggttc gacagcgacg cgatcagcgc
     3481 gcccgccgag gtgtggtcct cctgcgtctt gagcatcagg gccgaggcgt agagcagttt
     3541 gccgtcgtcc tccgattccg ggatcaggct tttcagcgcg ctcagcgagg ccagataatc
     3601 ctcccagccg accgcggcgc ccccattcct ggtctttcgg ccttcaaaat tggccagcac
     3661 cttcgcatag ccggttttca gcgtcccggc ggcttcggcg tcggcggcct tgacgtccgc
     3721 gccgaagccg agggcgagat cgtaggtcgc ggccttcgtc accgtcggca atcggccgat
     3781 cagcacgata ttgcccttca ccccgccggt ggagtcgtgc tgcgcgatca gggtctggcg
     3841 ctgcgtcagg tcggccacgc cgtcggtgac gccggtgaag ccgactgtgt gcgcggcgaa
     3901 cggcgcgctg gccttgagcg acagggcgac cttatcttgc cccccattgt tctgccacgc
     3961 attgagggcc gaagcgctgg ccgcgccttc gtcgtcgccg gaggtgttgg ccatgctggg
     4021 atcgaggatg acgtagggcg tcaccgggcc tttcagcgcc ttgacggtga cgcgcaccat
     4081 cacgctctgg cggtccgggt cggtgaacac gtccttgatg atgacgaagc ggccctgctt
     4141 gtcggtgttg gtcagccgat aggcgggcgc cagcggacgg cccgccgcgt ccgtgtcgat
     4201 ataggcgacg cggctgatcg tgtccgcatc ctcgaacgcg gtccagccgc cggacgcgtc
     4261 atggccggtg acggcgatct gcatttcgcg gatctgcgcc tcgtggatgc gcccgtacat
     4321 ggtttcggtc agcacgccct gcgccagcga gaaccacacc ttcgacaccg gccccgtgac
     4381 ggccttgtcg ctgaactggc cgtccttgtc ataggcttcg taggccgtgc cgatgccctg
     4441 cttgcccgaa taagaccagg gcgaggcgag gccggggcca ttgctggcgg gcgcgtcggc
     4501 gttcagcgtc ggattgtggt tctccggcgt cacggcctcg tcctgcgcgc gggcgcccgc
     4561 gccggaaagc gccgtcagca tcgccgccgc cgtcacaagg gcggttttca gcccgcgtcg
     4621 ctccactcgc tgtttcgccc gatctctcat acgcgtctcc tccggtccgc cgcagcggac
     4681 aacctgccgt tcgcggcgcg cttcggcggc gccggtgttt tggcggagac ataaacgcaa
     4741 agcgccgcgc ccgtcgcgtt gcatacgtat tcacgcgcgg cggcgcggcg tgaatacgaa
     4801 ttccgggcgc gcgcggggca aaaaaatccc cttgttcgtt gccggacaag gggaagttta
     4861 tgttgagaag ataccactta caaagcattc accctgtaat tggcgtatcc cggtcgcgtc
     4921 acaacaaccg gaatggcgga caggtcggat aagcggtgaa tacgccaccg gcatccgacc
     4981 tgaaaatcgc gatgtaaacg tttacggcgc ctatccaaaa caaaagcgtt cacaaatctc
     5041 ggctcgcaga gccgttggaa gcgctttcgg accgtcgtgg cgcgccataa gcgcctactc
     5101 catattcata aagcctaacc gcacttttgc caagatgcaa atttgaatac gtattcaaac
     5161 cgccggataa tcgcggcgaa ttgtcgccgc cgaaatttga gagcgctttc ataaatccgg
     5221 cgcgcccgtc cgcgcccggc ccggcggccg tcggggcgcg taaagccgtc ttgtcgaggc
     5281 ggacgaaatc gtgcgacagg aaggcgtatc ccggttcggc tttccgcgca gagccgcgca
     5341 gcaacaggac gtgcgtatgg acaagccatc cggcaagatc aggctcgagg atctggccaa
     5401 gatcgcgggc gtctcggtcg cgacggtgtc gcgcgcgctc aacgactcgc cggcggtcaa
     5461 ccgcgtgacc aagcgccgcg tctggaagat cgcgcgcgac cacaattacg tgctcaatca
     5521 ggacatgccc ttgtcgatgt ccggctcgca ggccaccatc gccatcgtca ttccgccgcc
     5581 gcacgggcgc gagggccgct tttccgaccc cttctatcag gagatgatct ccggcgtcgg
     5641 cgaggcggcg cgcgacatgg gctgcgacct tctgctgtcg cacctgacgc cgcgcagcca
     5701 gaacgacctc gtggccctga tgcagaacat caaggccgac ggggtgatct tcctgggcca
     5761 gagctatctg cacgaccgct tcaatcatct ggtcgagacg acgcgcaact tcatcgtctg
     5821 ggggcaggag ctgccggggc agaaatactg cacggtcggc tccaacaatc tgcagggcgg
     5881 cgcgcgcgcc accgcgcacc tggcgcggct ggggcggcgg cgcatcgcct atttcggcga
     5941 aaccgaaggc tcggaaatga agctgcgctt tcagggctat ctgaacgggc tggaggaggc
     6001 cggagtggaa ttcgatccgc gcctcgtcgt gccgacccat ttcgagctgg cctcggccga
     6061 ggcggcggtg ctgaacctga tccatcgcgg cgtcgccttc gacggcctgt tcgcctgctc
     6121 cgacatcatc gccttcggcg ccattcgcgg gctgatgcgc gccggacgcg acgtgccgcg
     6181 cgacgtgtcg gtggtgggct acgacaatat ccagctggcc agcctgaccc gtccggccct
     6241 gaccaccatc tcgcaggaca tggcgcgcgc gggcaagctg atggtgtcca agctgatcgg
     6301 ctcgatctcg aacgacgaga gccattcgga gatcgtgtcc accgaactgg tggtgcgcga
     6361 gtcgtgcggg gcgtaggaaa gcgcttctca ttttcatatt tttgcaaagt tttgcatcga
     6421 acgtcaaaaa tacgccgcga cgtcaactcg cgcttttggc ggtgcggacg gcgattatgt
     6481 cggcgtttac gtgcaaaagg cgcgctcctc aggccgaaat cgtagctttg aggccacatt
     6541 cttacgaata gtttagattc aggactgcaa aattttgcaa agcattgcaa aagtctggag
     6601 gctgagagaa tagtcgtccc gaggcagccg tcagagccgc cggtttaaag ggttgagatg
     6661 tcaacggaag ccttcgtcca cgcaaagacg aagctccgac tgccaaaacg caacaccagg
     6721 aggaacttct catgcacatt tccaagggtc gaaaggcagc gtaccgctca cgcctgctga
     6781 cctgcggcgc gacgctcgcc atcgtcgcgg cgtgcgcggc gacgggcgct caggcgcagg
     6841 acgcgaacgc agccaaaact caaactcagg cttcagggga cacgaccacc gttgtggtca
     6901 ccggcatccg caaggggatt caggacgcca tcaccgccaa gaagaagagc gcgcagatcg
     6961 tcgaagccgt gtcggccgag gacatcggca agctgccgga ccagtcgatc
//
LOCUS       DASVLL010000121         4264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_60182, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000121 DASVLL010000000
VERSION     DASVLL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60182"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VF402_01995"
     CDS             <1..1516
                     /locus_tag="VF402_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX7799091.1"
                     /translation="VAMSGQQVAVVCPTTLLARQHFKTFSQRFQGWPIRVRQLSRLVG
                     RKEADETRAGLKAGEIEVVIGTHALLSEQVAFRDLGLVIVDEEQHFGVKHKEKLKTFR
                     ADVHMLALSATPIPRTLQMALSGIRDMSIIATPPIDRIAVRTYVLPYDPVTLREALLR
                     EKYRGGQAYYVVPRISDLNDVADFLRAQVPEVRFVIGHGQLSPTALEDVMTAFYDGEY
                     DVLLSTTIVESGLDVPSANTLIVHRADMFGLAQLYQIRGRVGRAKTRAFAYLTTPQHK
                     TLSIASEKRLKVLQSLDNLGAGFQLASHDLDIRGGGNLLGHEQSGHIREIGVELYQQM
                     LEDAVAELREKSDRSLTDARAWSPQINTGAAVMLPESYIPDLNIRLALYRRVSEAEKM
                     EDREALASELIDRFGPIPEAAEQLLKVVGVKGLCREANVAKLDVGPKGAVIGFRNDSF
                     ANPMGLVRFIPTRPDWKLRPDQKLLVRGDWPDAAQRLAAAEKIMKELARLAKES"
     gene            complement(1513..2841)
                     /locus_tag="VF402_02000"
     CDS             complement(1513..2841)
                     /locus_tag="VF402_02000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7799092.1"
                     /translation="MLSFPARLLLLSRDESWSHALADDLDRLGLRTLCAAQSEAACVA
                     LGDLAIEAVLVDEGLADENDLVERLRAAAAPRRIAVIMMRRDDRHLVPDGWDMVFTRD
                     AHPQQILLSVEHLVRGCVCEEEYATRCASFAADMPDLDAQMASQPPLSLLSVGQPDPE
                     FLAMSHALRSQGVDVVAALTSYGAFDYLHDRNFDAVLLWGGEQPAEALAIAGGMRRNT
                     RLFHTPVLLRLNRLVEIDAGDAYLRGVNDIANPSATVAEIADRLTRLARGYRRRTTIR
                     KTLDAMRHRPEMDTGTGLFGRELFAAHLARLTEACRQRQRALSLCVLRIADTPEIARA
                     RAKGMLERAVPQIGSMVARLVRSEDSAGRLSNEVFAIALPATTLAQARNVGERISAVI
                     SCTAFDSGQGKPPFVVEFTIGAAELQPGETAAAALMRAAQNAGDTAKEAV"
     gene            complement(2950..3213)
                     /locus_tag="VF402_02005"
     CDS             complement(2950..3213)
                     /locus_tag="VF402_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX7799093.1"
                     /translation="MSSPSPDTPPDPPPDWSCVDARGHRCPVPSLRLRRALAQDAVAG
                     VRLFADDPMAQIDVPHFCNQNNYVLTAVHALDAGWAFDVRKPQ"
     gene            complement(3215..4120)
                     /locus_tag="VF402_02010"
     CDS             complement(3215..4120)
                     /locus_tag="VF402_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7799094.1"
                     /translation="MEQAHIDFDDLRAFFRVAETGSFAKAAQRLESSKSLISRRVARL
                     EETLAAQLLQRSARGALLTEAGQAYYEEARAAMAQLECAAESLSGAVGDISGQIRLTG
                     PVFFGSEYLTPALTEFARLYPNIELCVDYSDEKVDLIREGYDLATRVGHLPASSMTAR
                     LLCQSRRVIVASRAYLDGHPPILEPDDLGGQTILHYNGVNAQDLWRYGAGAEARAVAI
                     RPRLRSNNAYMLMEAVRAGLGLTLLPVYIVGRYVQSGEIDIVLGNYDWGVTPVHMLMP
                     PGRAPTRRVRTLIDFLALRFRNKFA"
BASE COUNT          733 a         1372 c         1484 g          675 t
ORIGIN      
        1 ggtggccatg tcgggccagc aggtcgccgt ggtctgcccg accaccctgc tggcgcggca
       61 gcatttcaag accttcagcc agcgctttca gggctggccg atccgggtgc ggcaactgtc
      121 gcggctggtc ggacgcaagg aggccgacga gacgcgcgcc ggtctgaagg cgggggagat
      181 cgaggtggtg atcggcacgc acgccctgct gtccgagcag gtggcgttcc gtgacctcgg
      241 cctcgtcatc gtcgacgagg agcagcattt cggcgtcaag cacaaggaaa agctcaagac
      301 cttccgcgcc gacgtgcata tgctggcctt gtccgcgacg ccgataccgc gcaccttgca
      361 gatggccttg tcgggcattc gcgacatgtc gatcatcgcc acgccgccga tcgaccgcat
      421 cgccgtgcgc acctatgtgc tgccctacga tccggtcacg ctgcgcgaag ccctgctgcg
      481 tgagaaatat cgcggtgggc aggcctatta cgtcgtgccg cgcatcagcg acctgaacga
      541 cgtggccgac ttcctgcgcg cgcaggttcc cgaggtgcgc ttcgtgatcg gccacggcca
      601 gttgtcgccg accgcgctgg aggacgtgat gacggccttc tatgacggcg aatacgacgt
      661 gcttttgtcg accaccatcg tcgaatcggg cctcgacgtg ccgagcgcca acaccttgat
      721 cgtgcatcgc gccgacatgt tcggtctggc gcagctctat cagatccgcg ggcgcgtcgg
      781 gcgggccaag acgcgcgcct tcgcctacct gaccacgccg cagcacaaga ctttgagcat
      841 tgcttcggaa aagcggctca aggtgctgca atcgctcgat aatcttggcg cgggctttca
      901 gttggccagc cacgatctcg acattcgcgg cggcggcaac ctgctgggcc acgagcagtc
      961 cggccatatc cgcgagatcg gcgtcgaact gtaccagcag atgctggagg acgcggtggc
     1021 ggaactgcgc gaaaagagcg accgcagcct gaccgacgcg cgcgcctggt cgccgcagat
     1081 caacaccggc gcggcggtca tgctgccgga aagctatatt cccgacctca atatccgcct
     1141 ggccctgtac cgccgcgtgt cggaggccga gaagatggag gaccgcgagg cgctggcgtc
     1201 ggaactgatc gaccgtttcg ggccgatccc ggaggcggcc gagcagttgc tgaaagtggt
     1261 cggcgtcaag gggctgtgcc gcgaggccaa tgtcgccaag ctggacgtcg ggccgaaggg
     1321 cgcggtgatc ggcttccgca acgacagctt cgccaacccg atggggctgg tgcgcttcat
     1381 tccgacgcgg cccgactgga agctgcgccc ggatcagaaa ctgctggtgc gcggcgactg
     1441 gcccgacgcg gcgcagcgtc tggcggcggc ggagaaaatc atgaaggaat tggcgcggtt
     1501 ggccaaggaa tcttaaaccg cctccttagc cgtgtcgccc gcgttctgcg ccgcgcgcat
     1561 cagggcggcg gcggcggttt cgcccggctg caattcggcg gcgccgatgg tgaactcgac
     1621 cacgaagggc ggcttgccct gaccggaatc gaaggcggtg caactgataa cggcggagat
     1681 gcgctcgccg acattgcgcg cttgcgccag ggtggtggcg ggcagggcga tggcgaacac
     1741 ctcgttcgac aggcggcccg cgctgtcttc cgagcgcacg aggcgcgcga ccatcgaacc
     1801 gatctgcggc acggcgcgct ccagcatccc cttggcgcgc gcgcgggcga tctcgggcgt
     1861 gtcggcgatg cgcagcacgc acaggctcag ggcgcgctgg cgctggcggc aggcttcggt
     1921 caaccgcgcc agatgcgcgg cgaacagctc gcgtccgaac aggccggtcc ctgtgtccat
     1981 ctccgggcgg tggcgcatgg cgtccagcgt cttgcggatg gtcgtccgcc gccggtagcc
     2041 gcgcgccagc cgcgtcagcc ggtcggcgat ctcggcgacc gtcgcgctgg ggttggcgat
     2101 gtcgttgacg ccgcgcaggt aagcgtcgcc cgcgtcgatc tcgaccagac ggttcagccg
     2161 cagcagcacg ggcgtgtgga acaggcgggt gttgcgccgc atgccgcccg cgatggccag
     2221 cgcctcggcg ggctgctcgc cgccccacag cagcacggcg tcgaaattgc ggtcgtgcag
     2281 gtagtcgaag gcgccgtagc tggtcagggc ggcgaccaca tccacgccct gcgaccgcag
     2341 cgcgtggctc atggccagga attccgggtc gggctggccg accgacaaca ggctgagcgg
     2401 cggttgcgac gccatctgcg cgtcgaggtc gggcatgtcg gcggcgaagc tggcgcagcg
     2461 ggtggcgtat tcctcctcgc acacgcagcc gcgcaccaga tgctcgacgc tgagcaggat
     2521 ctgctgcggg tgggcgtcgc gcgtgaacac catgtcccag ccgtcgggga ccagatggcg
     2581 gtcgtcgcgg cgcatcatga tgacggcgat gcggcgcggc gcggcggcgg cgcgcagccg
     2641 ctcgaccaga tcgttttcat ccgccaggcc ctcatcgacc agaaccgcct cgatggccag
     2701 atcgcccagc gcgacgcagg cggcctcgct ctgcgcggcg cagagggtgc gcaggcccag
     2761 ccggtcgaga tcgtcggcca gcgcgtgcga ccagctttcg tcgcggctca gcagcagaag
     2821 gcgcgccggg aaactcagca cggacgcacc gggcgcgaaa cggcgaggga gggaatcagg
     2881 atcatggtcg tcaccttaga tacgccaggt aaacattata acataaaaac cggtgcgcat
     2941 gaggccggtt cactggggct tgcgtacatc gaaggcccag ccggcgtcga gcgcatggac
     3001 ggcggtcagg acgtagttgt tctgattgca aaaatgcggc acgtcaatct gcgccatcgg
     3061 atcgtcggca aaaagcctga cgccggcgac ggcgtcctgc gccaatgcgc ggcgcaggcg
     3121 caggctcggc acgggacagc gatggccgcg cgcgtcgacg caggaccagt cgggcggcgg
     3181 atcgggcggc gtgtcaggcg aaggggacga catgtcaggc gaacttgttg cggaagcgca
     3241 gggccaggaa atcgatcagc gtgcgcaccc gccgcgtcgg cgcccgtccc ggcggcatga
     3301 gcatatgcac cggcgtgacg ccccagtcgt agttgccgag cacgatgtcg atttcgcccg
     3361 attgcacata acggccgacg atatagaccg gcagcagggt caggcccaga ccggcgcgca
     3421 ccgcctccat cagcatgtag gcgttgttgg agcgcaggcg cggacggatg gcgacggcgc
     3481 gcgcctctgc cccggcgccg taacgccaca gatcctgcgc gttgacgccg ttataatgca
     3541 ggatcgtctg gccgccgaga tcgtcgggtt cgagaattgg cggatggccg tccagatagg
     3601 cgcggctggc gacgatgacc cgccgcgact ggcagagcag gcgcgcggtc atgctcgaag
     3661 ccggcagatg gccgacgcgc gtcgccagat cgtagccctc acggatgaga tcgacctttt
     3721 cgtcgctata gtcgacgcat aattcgatgt tcggatacag gcgcgcgaat tccgtcaacg
     3781 cgggcgtcag atattccgag ccgaagaaca ccggcccggt cagccgtatc tggcccgaaa
     3841 tatcgccgac ggcgcccgac aggctttcgg ccgcgcactc cagttgcgcc atggccgcgc
     3901 gcgcctcttc gtaataggcc tgtccggctt cggtaagcag ggcgccgcgc gccgagcgtt
     3961 gcagcaactg cgccgccaaa gtctcctcca gccgcgcgac gcggcgggag atcagcgact
     4021 tggacgattc gagcctctgg gcggccttgg cgaaactgcc ggtctccgcg acacggaaga
     4081 aggcgcgaag atcatcgaag tcgatgtggg cctgttccat tttatgcaac accgtgatgc
     4141 gtttcgctgg gcttcctgaa acgaggctag cgaattatac cgcatccgac aagagtgagt
     4201 tggtgtggca ggcgtcattc acgcggggcg tatttccgca acggagatat ccaatgttca
     4261 tgac
//
LOCUS       DASVLL010000122         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_329125,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000122 DASVLL010000000
VERSION     DASVLL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329125"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1824)
                     /locus_tag="VF402_02015"
     CDS             complement(151..1824)
                     /locus_tag="VF402_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7799095.1"
                     /translation="MTIKFRILALVAGFALMASAITALGLVTINDYNHMLLTADRAHE
                     NAYRGEHLNRLVTGVVMDSRGVYMSQDTSEAAKYGAGIDDQLGQIQTLLTQWKGDLKP
                     GELPEFDGVYQKAQEFVAFRRELARLGRDVSPAAANVQGNNDANRNNRKDFQADIDAM
                     VKHIQTNLDASQTAMQHYKNERSGQFLLVAAAGILLLVGASLWIAIRSISRPLQRVTD
                     SVMRISEGAYDTAIPEARGKDEVSSLWRSIRTLRDRAQEGETLKAHQAEEDERVAANM
                     RAERNRIAAEFEQSMGELARRFAASSRTVADSARELADNAGRAVGLIQSVNGAAVDAA
                     NNVQSVAAATEELSASVHEINQQVARTSQVSQMAVSEAAKTDDAIRSLSAAAGQIGEV
                     INLIQAIAAQTNLLALNATIESARAGEAGKGFAVVASEVKQLAQQTAKATDDIRSKIA
                     EIQSATHITVDSIGAIVRTIEDIGELTTAIAASVEQQGAATQEIASNTNRAADGTRDV
                     TVHMTTVQDGAEGAGQSAQSLLALSADLEQRSEDMHTEVAQFIERLRAA"
     gene            2115..>2254
                     /locus_tag="VF402_02020"
     CDS             2115..>2254
                     /locus_tag="VF402_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HEX7799096.1"
                     /translation="MKTRAAVAFEAKKPLEIVEVDLEGPKAFEVLVEIRATGICHTDA
                     YTL"
BASE COUNT          344 a          691 c          789 g          430 t
ORIGIN      
        1 ccgacgggct ggaggggttg aaggggtagg gggtagataa gggggaccgc acgaagccga
       61 aggctgagat gcggtggtgg ggcgtcctgc tttaggcgcg aaaaaggcgg ctccgccgga
      121 gccgcctttc gtctgtcccg cctccggcgc ttacgccgcg cgcagccgtt cgatgaattg
      181 cgcgacctcg gtgtgcatgt cctcggagcg ttgctccaga tcggcggaca gggccagcag
      241 cgactgggcc gactggcccg cgccctcggc gccgtcctgc acggtggtca tgtgcacggt
      301 gacgtcgcgc gtgccgtcgg cggcgcggtt ggtgttgctg gcgatttcct gcgtcgcggc
      361 gccctgctgc tcgaccgagg cggcgatggc ggtggtcagt tcgccgatgt cctcgatggt
      421 cctgacgatg gcgccgatgc tgtcgaccgt gatgtgggtg gcggactgga tttcggcgat
      481 cttggagcgg atgtcgtcgg tggccttggc ggtctgctgc gccagttgct tgacctccga
      541 cgccacgacc gcgaagccct tgcccgcttc gcccgcgcgc gccgattcga tggtggcgtt
      601 gagcgccagc aggttggtct gggccgcgat ggcctgaatc aggttgatga cctcgccgat
      661 ctgaccggcg gcggcgctga gcgagcggat cgcgtcgtcg gtcttggcgg cttcggacac
      721 ggccatctgc gacacctgcg aggtgcgcgc gacctgctgg ttgatctcgt ggacgctggc
      781 cgacagctcc tcggtggcgg cggcgacgct ctggacgttg ttggcggcgt cgacggcggc
      841 gccgttgacc gactggatca ggccgacggc gcgcccggcg ttgtcggcca gctcgcgcgc
      901 cgaatcggcg accgtgcgcg acgaggcggc gaagcggcgg gccagttcgc ccatgctctg
      961 ctcgaactcg gcggcgatgc ggttgcgctc ggcgcgcatg ttggcggcga cgcgctcgtc
     1021 ctcctcggcc tgatgcgcct tgagcgtctc gccctcctgc gcgcgatcgc gcagggtgcg
     1081 gatcgagcgc cacagcgacg acacctcgtc cttgccgcgc gcttcgggga tggcggtgtc
     1141 gtaggcgcct tcggagatgc gcatcacgct gtcggtcacc cgttgcagcg gccgcgagat
     1201 cgagcggatg gcgatccaca gcgatgcgcc gaccagcagc aggatgcccg cggcggcgac
     1261 cagcaggaac tggcccgaac gctcgttctt atagtgctgc atggccgtct gcgaggcgtc
     1321 gagattggtc tggatatgct tgaccatcgc gtcgatgtcg gcctggaagt ccttgcggtt
     1381 gttgcggttg gcgtcgttat tgccctgcac gttggccgcc gccggcgaga cgtcgcggcc
     1441 cagccgcgcc agttcgcggc ggaaggcgac gaattcctgc gccttctggt aaacgccgtc
     1501 gaattccggc agctcgcccg gcttgaggtc gcccttccac tgtgtcagca gggtctggat
     1561 ctggcccagc tgatcgtcga tgcccgcgcc gtacttggcg gcctcgctgg tgtcctgcga
     1621 catatagacg ccgcgcgaat ccatcaccac gccggtgacg agacgattca ggtgttcgcc
     1681 gcgataggcg ttctcatgcg cccggtcggc ggtgagcagc atgtggttgt aatcgttgat
     1741 ggtgacgagg ccgagggcgg tgatcgccga agccatgagg gcgaaaccgg cgaccagcgc
     1801 cagaatgcgg aatttgatgg tcatttttac gactccccag aacgcggaat actcatacgc
     1861 ggaacagatg cgcgtcagtg tagagtggca gggttaagac tttatgattc tacaacctta
     1921 gagcgccctt cgatccgatg ggatcggatc ggcgctctaa gcctttgttt tgtcgctctg
     1981 ccttatccaa aaagtggagc cactttttgg ggaaacgcta taaagtctga taacgcgccg
     2041 aacgcggttt tgacttgcct tcgtacaggc gccgccgcac aaaaccgtca aaaccccagc
     2101 cggagaaaag cgccatgaaa acccgcgccg ccgtcgcctt cgaggcgaaa aagccgctcg
     2161 aaatcgtcga ggtcgatctg gaaggcccca aggcgttcga ggtgctggtc gagatcaggg
     2221 cgaccggcat atgtcatacc gacgcctaca cgct
//
LOCUS       DASVLL010000123         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_7611, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000123 DASVLL010000000
VERSION     DASVLL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7611"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1976)
                     /locus_tag="VF402_02025"
     CDS             complement(75..1976)
                     /locus_tag="VF402_02025"
                     /inference="COORDINATES: protein motif:HMM:NF022752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX7799097.1"
                     /translation="MRRLAVLTTACAGLGAGAAGAAPIVIHTATVDISVDPASLRIDA
                     TTPDGQALNLMPALHAPEAAAPVRVGAAWRWSDNEGRILTVSGEDGHVRVQIAAPDGQ
                     ALSWPLPVASDGDWITPDGEGMALPVQDAFWRHVERREHCLDAVAGLSFPAWSHLTPE
                     RAVVYALGDGLRSKLCLRDDQGLQARLTHSFDEGANRLELLIAVAPPDPLAPALFYRA
                     LLKARGRFRLLRDKAVPDLDRLYGAPQAYLWGDGRDMAMLDRLKALGIARINLSYDQD
                     PQTDTHLVTPAFLKKARALGYLAGPYDTFDNAQPDDNDAAPYVHWGKALYPSGCIRDA
                     AGRIKAGFANTGCEMSSEALVRAPGPFVPGARYKQHKADGADQVFDDVDATGEFFHDD
                     SPDHPMTMTQDRANRLARLNLAMRYGLVIGSEDVHAWSAGAVHYSHGGAQSHLSAVWP
                     VQSDRKRFGGWWPGDHLPLFFASFQQTPDEARLLFGPGDRLPLFEAVFHDSVISSDRW
                     EFGLMKVAGVERARFAMGLLYGTPSMWPLDQKELARVGPWLKAAQDDFRIAHGWNAPV
                     ALTGFRWETPDRLVQTATYADGRRITANFGAAPYDGLAGGCVRVARPGPHGQDWAARD
                     LCPPPLPAP"
     gene            2115..2342
                     /locus_tag="VF402_02030"
     CDS             2115..2342
                     /locus_tag="VF402_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799098.1"
                     /translation="MIKISNKMLLPLAAAALCLGASACSKTSETTTTTETTTSEMAPA
                     DNGGAMSDMADSASAGAVTTTTTTTTASSSM"
BASE COUNT          387 a          864 c          795 g          371 t
ORIGIN      
        1 gcgggcaagt cgatcaacgg cgccccgctc agcatcgacg gcggctggac ggcggcctga
       61 tcagggttgc gccattatgg cgccggtaag ggcggcgggc acagatcgcg cgccgcccaa
      121 tcctgtccat gcgggcccgg cctcgccacg cgcacgcagc cgccggccag cccgtcatag
      181 ggcgccgcgc cgaaattcgc cgtaatccgc cgcccgtcgg cataggtcgc ggtctggacg
      241 aggcggtccg gcgtctccca gcggaagccg gtcagcgcga ccggcgcgtt ccagccatgc
      301 gcgatgcgga aatcgtcctg cgcggccttc agccacggcc cgacccgcgc cagctccttc
      361 tggtccagcg gccacatgct cggcgttccg tacaacagcc ccatcgcgaa acgcgcgcgc
      421 tccacgcccg ccaccttcat caggccgaac tcccagcggt cgctggaaat gaccgagtcg
      481 tgaaagacgg cctcgaacag cggcaaccgg tcgccggggc cgaacaaaag ccgcgcctcg
      541 tccggcgtct gctggaagga tgcgaaaaag agcggcagat ggtcgcccgg ccaccagccg
      601 ccgaaacgct tgcgatcgct ctgcaccggc cagaccgcgc tcaggtgcga ctgcgcgccg
      661 ccgtggctgt aatgcacggc cccggcgctc cacgcatgca cgtcctccga tccgatcacc
      721 agtccgtagc gcatggcgag attcagccgc gccagccggt tggcgcggtc ctgcgtcatg
      781 gtcatcggat gatcggggct gtcgtcgtgg aaaaactcgc cggtcgcgtc gacgtcgtcg
      841 aacacctgat cggcgccgtc ggccttatgc tgcttatagc gcgcgccggg gacgaacggg
      901 ccgggcgcgc ggaccagcgc ctcgctcgac atctcgcagc cggtattggc gaagcccgcc
      961 ttgatcctgc ccgccgcgtc gcggatgcag ccggacggat agagcgcctt gccccagtgc
     1021 acgtagggcg cggcgtcgtt gtcgtccggc tgggcgttgt cgaacgtgtc gtaaggcccg
     1081 gccagatagc cgagcgcgcg cgcctttttc aggaaggcgg gcgtgaccag atgcgtgtcg
     1141 gtctgcggat cctgatcgta gctgagattg atgcgcgcga tgcccagcgc cttgagtcgg
     1201 tcgagcatgg ccatgtcgcg cccgtcgccc cacagatagg cctgcggcgc gccgtacagg
     1261 cggtcgagat cgggaaccgc cttgtcgcgt aaaagacgga accgcccgcg cgccttgagc
     1321 agggcgcggt aaaacagggc gggcgccagc ggatcgggcg gcgccacggc gatcagcagc
     1381 tccagacggt tggcgccttc gtcgaaactg tgcgtcagcc gcgcctgcaa gccctgatcg
     1441 tcccgcaggc acagcttcga gcgcaggccg tcgcccagcg catagacgac ggcgcgctca
     1501 ggcgtcagat ggctccacgc cggaaaggac agccccgcca cggcgtcgag gcagtgttcg
     1561 cgtctttcga catggcgcca gaacgcgtcc tgcaccggca gggccatgcc ttcgccgtcc
     1621 ggcgtgatcc agtcgccgtc gctcgcgacc ggcagcggcc aggacagggc ctgcccgtcc
     1681 ggcgcggcga tctgcacgcg cacatgcccg tcctcgccgg acacggtcag gatgcggccc
     1741 tcgttgtcgc tccagcgcca cgccgcgccg acccgaacgg gcgccgccgc ttccggcgca
     1801 tgcagggcgg gcatgaggtt cagcgcctgc ccgtccgggg tcgtcgcgtc gatgcgcagg
     1861 ctggccggat cgaccgagat gtccaccgtc gcggtgtgaa tcacgatcgg cgccgccccg
     1921 gctgcgcccg cgcctaagcc tgcgcacgcc gtcgtcagga cggcgagccg tcgcatgatg
     1981 tcgcgccgga aagtcatttt tcgccccgcc cgctttttat ccctttttta tcagcagcgg
     2041 gcggtctccg caaagcttct ctatttcggc ttggccatag tcccgcttca cctgtttgtg
     2101 aatggagagg gactatgatc aagatttcca acaagatgct gctgccgctc gccgccgccg
     2161 ccctgtgcct gggcgccagc gcctgctcga agacgagcga gacgacgacc acgacggaga
     2221 ccacgacctc cgaaatggct ccggcggaca atggcggcgc catgtccgac atggccgaca
     2281 gcgcctcggc gggcgccgtc accaccacga ccacgacgac gaccgcctcg tcttcgatgt
     2341 aagccttacg ggctttttgc atcccccggc ctgttaaggc ggcgcaaaaa agccggtttg
     2401 aacgcaaaat ccctctc
//
LOCUS       DASVLL010000124        10913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_114365,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000124 DASVLL010000000
VERSION     DASVLL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114365"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VF402_02035"
     CDS             complement(<1..1312)
                     /locus_tag="VF402_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7799099.1"
                     /translation="MGNWNFKALLRNKSMLAGGVVGLVLGAGVAGAAVSGLGDAPGHA
                     LGFDGARLVRTADLTPVNPPAGAPMSFADLIEHVSPAVVSIETTGKVKVDASNFPNLP
                     GFSFPGQDGGQPKEQEVRGAGSGFFISADGYVVTNNHVIDGADDITVKLTNDKELKAK
                     VVGRDPATDLAVLKVEGNDFPFVRFELEHKPRVGDWVVAMGNPFNFSNTATAGIVSAY
                     GRDLHDSGSGDYVDYLQIDAAINRGNSGGPTFDMYGKVIGINTAIVTPSGANAGVGFA
                     IPADVAAKITDKLMKGETIQRGYVGITIQPLDKDTAEALGITSLDGAYVTDVNKDGPA
                     GKAGMQPGDIVKTVNGAEVKSPTDLTRRIADVQVGEKVNVGVLRAGKMINLTVTAALR
                     PSEAQLSSLLNGNGGGDDGAGPNAPAGAAAPVIGLSVKAITPDAR"
     gene            complement(1442..1870)
                     /locus_tag="VF402_02040"
     CDS             complement(1442..1870)
                     /locus_tag="VF402_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX7799100.1"
                     /translation="MKRLLLLFLFLPAVAHADPAHDEARAQRLFAEVRCVQCQSESIA
                     DSDAAIAADMRREVRADIAKGMSDGQIRTSLYDHYGDYVLFRPRLSKSNLVLWTLPPL
                     IAIIGAVLLFMRRKTSVIPETSALSDSEQKKLLELLKKHD"
     gene            complement(1867..3831)
                     /locus_tag="VF402_02045"
     CDS             complement(1867..3831)
                     /locus_tag="VF402_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX7799101.1"
                     /translation="MIDEIGNFTLILAFCFSLAQAGLVGLGQARRWRGLERWAEGVSL
                     AAAVSVLISFAVLIFAFVRSDFSVANVYQHSHTDKPLLYKISGAWGSHEGSMLLWCSI
                     LLSYGALITLLGRGLPEGLRLKALGVQGLLGAAFVGFTLFSSNPLDRLDPAPVQGLSL
                     NPALQDPALAFHPPMLYLGYVGFSVVFSFAVAALIEGRLDRAWARWVRPWVLAAWSFL
                     TLGITLGAFWAYYELGWGGWWAWDPVENASFMPWLAATALLHSAIVMEKRDALKAWTV
                     FLGLLAFTFSMMGAFLVRSGLLTSVHAFALDPARGVLLAIILFLTAGSGFALFALRAP
                     ALAPGGLFAPVSREASLVLNNLLLSAALCSVFLGTLYPILMQALLDKTMSVGAPYYMA
                     VFAPIMAVALLILPLSPLLAWKRADLKGALQRLAAAFGAAVLAAVVGTFFYHGPWLSE
                     IAFGLGLLVGFWLIAGTIKALLLRIDFKPSRLRVAPLAFYGMILAHAGLGVFVLGAVV
                     EGHERVSVQQPMAPGDSVRLGRYEMRFTGVSSRPGKNYDAQIGHFDISDAQGRRVCAA
                     TPQRRFYPASNDMQSKVALCFQPLSDLYVVMAQPSVTAAGKAGYQVRFFVNPWVRLIF
                     LGPVLMALGGVLSLSDRRLRLGVAAKRRAA"
     gene            complement(3848..4339)
                     /gene="ccmE"
                     /locus_tag="VF402_02050"
     CDS             complement(3848..4339)
                     /gene="ccmE"
                     /locus_tag="VF402_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX7799102.1"
                     /translation="MGFWPKSHKARRRLTVFLIAAPVLIAAVALSLYALRGTVVYFYT
                     PAQLDAARAPEGRTIRLGGLVKPGSIVKSPDGTVRFVVQDKLAQTPVSYKGDLPDLFR
                     EGQGVVCEGVWRGGALTATQVLAKHDEKYIPKNVEDELKKQGEWRPESGMTTSALPQR
                     PFS"
     gene            complement(4443..5525)
                     /gene="ccmI"
                     /locus_tag="VF402_02055"
     CDS             complement(4443..5525)
                     /gene="ccmI"
                     /locus_tag="VF402_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX7799103.1"
                     /translation="MPLLLFVCVLIAALLAALAILWRRMAPAPAPLAQEKALYAGFLA
                     DVERRLAAGDLDAQAADEERTAAARALLKAGEAAPGAQVRPGYGYAALALTGVLSLVL
                     YGLFGHPGLKDQPFRARLAEWTHKAGTDPDALSPQALALVLRQGRAEHAQDPAYWAFL
                     GRIDMLAGNNYQGAQDYEKARALDPARFDHWSELGETLTFVAGGNVGPDAQAAFETAL
                     ARDPKDARAHFYLGQAAAAAGKYDAARGHYEAALAAMDAHDVSRPMVRQALDALAPAQ
                     KAADAMKARVEGMVASLSAQLQADPDNPDGWARLIRSYDVLGDAQARAKAVAGMQAHY
                     RDRPAVAADILTKAQAAVGAEDTGGR"
     gene            complement(5571..6956)
                     /locus_tag="VF402_02060"
     CDS             complement(5571..6956)
                     /locus_tag="VF402_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799104.1"
                     /translation="MAAPGRTIDPHRLTLLQGLIRSLPAASLRALETALGLSKDAALA
                     GVRDLIAVEMESRQVRDAVFQPFLPLFEARRDGLEGVRFPRRFLSNIWNGLAAREADL
                     FREAFATLRALRPEDPTPVVFFRLTKAGADLCRAEPEAVAGSAPEARNEAVELARYFD
                     LHRIIRQTLSRMPEFMGRIDAEKAAALRVLFKDAAEFEDESGCRFLELIFANLEDGGQ
                     IIKFMATVADRPKDRFLAESELAVFGERILAHIEARVAALRQEMGTRTRPCEDMGLAA
                     QHVAQALAQLQSFEHYVELARDGPWGRRVAAAHKEVAALVEQVMGGADKQLAQILPMR
                     SERVHGRLKRETPDLDAEVAPARLEQALQVMNFIRQARVIAVSGGFSSLHAKTTQALE
                     AYMDDWFGHLLSDANSGDLSDVDRAAEAFERVAGLMAALCGEDKARTARRRVAASELF
                     KPRSGANGDAA"
     gene            complement(7056..8504)
                     /locus_tag="VF402_02065"
     CDS             complement(7056..8504)
                     /locus_tag="VF402_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7799105.1"
                     /translation="MSAAESAPRRGLDPRRFWRRLLRILSARDSLVGRLIRLASTYFT
                     VALIVTGIALTAYFHEVTMHRFQERVGQIADSLFSVTNIDADGVDTSRFVDARSAIVH
                     SGLYWQVGEVGANGAIVSAVTSRSLWDEQLPLPPGVLRDARAHSGSSVFYDGMGPVNE
                     PLRVAVIYSRVNNKPYVFMAGEDSSPVEHDVRTFALLTAAALLVLALGALAAIFIQVR
                     VGLRPLFDLTDEIAHVQSGEQQRLTKTYPAEIRPVAHQLNAFLDHAQEVVERQRMHVG
                     NLAHALKTPLSVLMTSAGDSGDALAQTVRKQAETMRAQVDHHLRRARAAARSKAMGER
                     TPVEPVLDELAVMLEQVFQDKGVVVDWRAPDDLAFRGERQDLQEIAGNLMENACIWCR
                     RKVMIHAEFDAAEQTVSLSVEDDGPGLAESRFDEVLKRGARLDESVPGSGLGLSIVDE
                     LVRAYGGTLSFGRSGWGGLKVTAHLPGGRTAS"
     gene            complement(8501..9181)
                     /locus_tag="VF402_02070"
     CDS             complement(8501..9181)
                     /locus_tag="VF402_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7799106.1"
                     /translation="MRILLAEDDPDLARQLKTSLSDAGYVVDHAPDGEEAHFLGDTEP
                     YDCVILDLGLPKIDGVSVLERWRRDGKTTPVLILTARGAWSDKVSGFDAGADDYLTKP
                     FHTEELLARLRALVRRAAGHAQPMLTCGGLRLDPRAARASVNGEPLRLTSLEYRLLHY
                     LMLHQNRVISRTELVEHLYDQDFDRDSNTIEVFMGRLRKKIGGDRIETVRGLGYRLKP
                     LHDEVGSL"
     gene            complement(9181..9576)
                     /locus_tag="VF402_02075"
     CDS             complement(9181..9576)
                     /locus_tag="VF402_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799107.1"
                     /translation="MKRSALLLSLMLTAAPLAAAWASCVQAAPYYDAADQHRRRGSND
                     DDRRPPAPRPRYDDDQRQPQQPAYRPQPDENRRDDRINRAIAAGRARGRVLDAGQQDG
                     STFWVRVDTGHGRVDLLVDGQTGRVVGER"
     gene            complement(9667..10860)
                     /locus_tag="VF402_02080"
     CDS             complement(9667..10860)
                     /locus_tag="VF402_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX7799108.1"
                     /translation="MRLGKSVAMTAALCLASAALVTPSYAQSSIRRKTEEKKAEIPTC
                     SHRIGTLAIHEPDNRWWEGLGLESPEALLKIFVQRSGCFTLVDRGKGFEMAQQERALA
                     AGDALQGGANIGGGQVRAADYILVPDIVSKNGNSGGTNIGGLLGGFIGGGVGMLVSGI
                     SINSKTADVVLSITNVRTSEQVAMEEGHGSKTDVGFSLGGAGGGFGGFGAAPQKGGDL
                     RLKLDIDLLDTIVGNTRRVLLSDGRTVDVNIPKGAKDGQTLRLKGQGAPSPNGRGPHG
                     DALVELHLKPHPVFRLEGHDLHMDLYVSLPDAVLGGKVQAPTPDGPVSLSIAKGTNSG
                     AIMRLKGRGAYDGKGAARGDLFAHVVIALPDKADGVPTDIQADLTALAEKWRGKGGYT
                     PSRRK"
BASE COUNT         1851 a         3884 c         3506 g         1672 t
ORIGIN      
        1 ggcgcgcgtc cggcgtgatc gccttgacgc tgaggccgat gaccggcgcg gcggcgcccg
       61 ccggagcgtt cggaccggcg ccgtcgtcgc ctccgccgtt gccgttgagc aggctgctca
      121 actgagcctc ggacgggcgc aacgccgccg tgacggtcag gttgatcatc ttgcccgcgc
      181 gcagcacgcc gacattgacc ttttcgccga cctgaacgtc ggcgatgcgg cgcgtcaggt
      241 cggtcgggct cttgacctcg gcgccgttga ccgtcttgac gatgtcgccc ggctgcatcc
      301 cggccttgcc ggcggggccg tccttgttga cgtcggtgac ataggcgccg tccaggctgg
      361 tgatgcccag cgcctcggcg gtgtccttgt cgagcggctg gatggtgatg ccgacatagc
      421 cgcgctggat cgtctcgccc ttcatcagct tgtcggtgat cttggccgcc acgtccgccg
      481 gaatggcgaa gccgacgccc gcattggcgc ccgacggcgt gacgatggcg gtgttgatgc
      541 cgatcacctt gccgtacatg tcgaaggtcg ggccgccgga attgccccgg ttgatggccg
      601 cgtcgatctg gagatagtcg acatagtcgc cgctgcccga atcgtgcagg tcgcgcccgt
      661 aggccgacac gatgcccgcc gtggcggtgt tggagaagtt gaacggattg cccatcgcca
      721 cgacccagtc gccgacgcgc ggcttgtgct caagctcgaa gcgcacgaac gggaagtcgt
      781 tgccctccac cttcagcacc gccaggtcgg tggccggatc gcggcccacc accttggcct
      841 tcagctcctt gtcgttggtc agcttgaccg tgatgtcgtc ggcgccgtcg atgacgtggt
      901 tgttggtgac gacatagccg tccgccgaga tgaagaagcc cgaaccggcg ccgcgcacct
      961 cctgttcctt gggctggccg ccgtcctggc cggggaagga gaagccgggc aggttgggga
     1021 agttgctggc gtcgaccttg accttgccgg tcgtctcgat cgacacgacg gcgggcgaga
     1081 catgctcgat caggtcggcg aacgacatcg gcgcgccggc gggcggattg accggcgtca
     1141 ggtccgccgt cctgaccagc cgggcgccgt cgaagccgag cgcatggccc ggcgcgtcgc
     1201 cgagccccga aaccgccgcc ccggccacgc ccgcgccgag caccagcccg accacgccgc
     1261 ccgccagcat cgacttgttc ctgagcagag ccttgaagtt ccagttcccc atattcgcca
     1321 atccttgtcc tagccgaacg cttcggctgc atctgagcgc gtccgacgcg caaagacaac
     1381 cgaaaaacgc gcaattccac gccttcggac taaactaaag cgatacggca aaatcgtgac
     1441 gtcaatcgtg cttcttcaag agttccagaa gttttttttg ctcactgtcg ctgagggccg
     1501 aagtctctgg aatcacggag gttttgcgcc gcataaacaa cagaacggcg ccgattatgg
     1561 cgatcagcgg cggcagcgtc cacaaaacaa ggttgctttt cgacagtctg ggccgaaaca
     1621 ggacataatc gccatagtgg tcgtaaagcg acgtgcggat ctgcccgtcg ctcatgccct
     1681 tggcgatgtc ggcgcgcacc tcgcggcgca tgtcggcggc gatggcggcg tcggaatcgg
     1741 cgatgctttc gctctggcac tgcacgcagc gcacctcggc gaacagcctc tgcgcccgcg
     1801 cctcgtcatg cgccgggtcg gcatgggcga cggcgggcag aaagagaaag agcagcagca
     1861 gacgcttcac gccgccctcc gtttcgccgc cacgcccagc cgcaaacgcc ggtcgctcag
     1921 cgacagaacg ccgcccagcg ccatcagcac cgggccgagg aagatcagcc gcacccacgg
     1981 attgacgaag aagcgcacct gataccctgc cttgcccgcc gccgtgacgc tgggctgggc
     2041 catgacgacg taaaggtcgc tgagcggctg gaagcacagc gcgaccttgg actgcatgtc
     2101 gttggaggcc gggtagaagc ggcgctgcgg cgtcgcggcg cagacgcggc ggccttgcgc
     2161 gtcgctgatg tcgaaatggc cgatttgcgc gtcgtaattc ttgcccggcc gcgacgagac
     2221 gcccgtgaag cgcatctcat agcggcccag acgcacggaa tcgcccggcg ccatcggctg
     2281 ctgcacgctg acccgttcat gcccctcgac cacggcgccc agcacgaaga cgccgaggcc
     2341 cgcatgggcc aggatcatgc cgtaaaaggc cagcggcgcg acgcgcagcc gcgacggctt
     2401 gaaatcgatg cgcagcagca acgccttgat cgtccccgcg atcagccaga acccgaccag
     2461 caggccgagg ccgaaggcga tctcgctcag ccacggtcca tgatagaaga aggttccgac
     2521 gaccgccgcc agaaccgccg cgccgaacgc ggcggccagc cgctgcaagg cgcccttgag
     2581 gtcggcgcgc ttccacgcca gcagcggcga cagcggcagg atcagcagcg ccacggccat
     2641 gatcggcgcg aacaccgcca tgtaataggg cgcgccgacc gacatggtct tgtccagcag
     2701 cgcctgcatc aggatcgggt acagcgtgcc gaggaacacc gaacacagcg ccgccgacag
     2761 cagcaggttg ttcagcacca gcgaggcttc gcggctgacc ggcgcgaaca gcccgcccgg
     2821 cgccagcgcc ggcgcgcgca gggcgaacag cgcaaagccc gaccccgccg taaggaacag
     2881 gataatggcc agcaaaacgc cgcgcgccgg atcgagcgcg aaggcatgca ccgaggtcag
     2941 aagccccgag cgcaccagaa aggcgcccat catcgagaag gtgaaggcca aaaggccgag
     3001 aaacaccgtc cacgccttga gcgcgtcgcg cttctccatc acgatggcgc tgtgcagcag
     3061 ggcggtcgcc gccagccacg gcatgaagga ggcgttctcg accggatccc acgcccacca
     3121 gccgccccag ccgagttcgt aataggccca gaaggcgccg agcgtaatgc ccagcgtcag
     3181 gaagctccac gccgccagca cccacggcct gacccagcgc gcccaggcgc ggtccagccg
     3241 cccctcgatc agcgcggcga cggcgaacga gaacaccacc gaaaagccga catagccgag
     3301 atagagcatc ggcggatgga aggccagcgc cggatcctgc aaggccggat tgagcgacag
     3361 cccctgcacc ggcgccggat cgagccggtc gagcggattg gacgaaaaca gcgtgaagcc
     3421 gacaaaggcg gcgcccagaa gcccctgcac gcccagcgcc ttgagccgca gaccttcggg
     3481 caggccgcgc cccagcagcg tgatcagcgc gccatagctc aggaggatcg aacaccacag
     3541 cagcatcgag ccttcatggc tgccccacgc gccggagatc ttgtacagca gcggcttgtc
     3601 ggtgtgcgaa tgctgataga cgttggcgac cgaaaagtcc gagcgcacga aggcgaagat
     3661 cagcaccgcg aagctgatca gcacgctgac cgccgccgcc agcgatacgc cctcggccca
     3721 gcgctcaagc ccgcgccatc gccgcgcctg tcccaacccg accagccccg cctgcgccag
     3781 cgaaaaacag aaggcgagga tcagggtgaa attgccgatc tcatcgatca tggtcttggc
     3841 cgctccctca cgaaaacggc ctctgcggca gcgcggacgt cgtcatgccg gactccggcc
     3901 gccattcgcc ctgcttcttc agttcgtcct cgacgttttt gggaatatat ttctcgtcat
     3961 gcttggccag cacctgcgtc gccgtcagcg ccccgccgcg ccagacgccc tcgcacacca
     4021 cgccctgccc ttcgcgaaac aggtcgggca aatcgccctt ataactgacc ggggtttgcg
     4081 ccagcttgtc ctgcaccacg aagcgcaccg tcccgtcggg cgacttgacg atgctgcccg
     4141 gcttgaccag accgcccagc cggatcgtgc gcccctccgg cgcacgggcg gcgtcaagct
     4201 gcgcgggcgt atagaaatag accaccgtgc cgcgcagcgc gtaaaggctc agcgccaccg
     4261 ccgcgatcag caccggcgcg gcgatcagaa acacggtcag cctgcgccgc gccttatgcg
     4321 atttcggcca gaaccccatt acgcttctcc cttcgggatc agcgtaatga tcttagctcg
     4381 cgctaacgct tcgcttcttg agcggctttt tatttgcgct aacgcttcgc ttcttgagcg
     4441 cattaacgtc ctcccgtatc ttccgcgccg accgccgcct gcgccttggt caggatgtcc
     4501 gccgcgaccg cgggccggtc gcgatagtgc gcctgcatcc cggccacggc cttcgccctg
     4561 gcctgcgcgt cgcccagcac gtcatacgac cggatcagcc gcgcccagcc gtcgggattg
     4621 tcgggatcgg cctgcaactg cgccgacagc gacgcgacca tgccttcgac gcgcgccttc
     4681 atcgcgtccg ccgccttttg cgccggggcc agcgcgtcga gcgcctgccg caccatcgga
     4741 cggctgacgt cgtgcgcatc catcgcggcc agcgccgctt cgtaatggcc gcgcgccgcg
     4801 tcgtatttgc ccgccgccgc cgccgcctgc cccagataga agtgcgcgcg cgcgtccttc
     4861 ggatcgcggg ccagcgccgt ctcgaacgcg gcctgcgcgt ccggcccgac attgccgccc
     4921 gccacgaagg tcagcgtctc gcccagttcc gaccagtgat cgaaacgcgc cggatcgagc
     4981 gcccgcgcct tttcgtaatc ctgcgcgccc tgataattat tgcccgccag catgtcgatg
     5041 cgccccagga acgcccagta ggccgggtct tgcgcatgct ccgcccgccc ctgccgcaac
     5101 accagcgcca gcgcctgcgg cgacagcgcg tcgggatcgg tcccggcctt gtgcgtccat
     5161 tcggccagcc gcgcccggaa cggctgatcc ttgaggcccg gatggccgaa caggccgtaa
     5221 aggacaaggc tcagcacacc cgtcagcgca agcgccgcat agccgtaacc cggcctgacc
     5281 tgcgcgcccg gcgccgcctc gcccgccttt aaaagcgcgc gcgccgccgc cgttctttcc
     5341 tcgtccgccg cctgcgcgtc gagatcgccc gccgccagcc tgcgctcgac atcggcgagg
     5401 aagcccgcat acagcgcctt ttcctgcgcc aaaggcgcgg gcgcgggcgc catgcggcgc
     5461 cacaggatcg cgagagcggc cagcagggcg gcgatcagaa cgcagacgaa aaggagcaac
     5521 ggcatggtcg ccgacataat agcaattcgc gcggcttgga aagggccaga tcaggcggcg
     5581 tcgccatttg ccccggatcg cggcttgaac agctcgctgg cggccacccg gcggcgcgcg
     5641 gtcctggcct tgtcctcacc gcacagcgcc gccatcagcc cggccacgcg ctcgaacgcc
     5701 tcggcggcgc ggtcgacatc gctcagatcg ccgctgttgg cgtcgctcag caaatggccg
     5761 aaccagtcgt ccatataggc ttccagcgcc tgcgtcgtct tggcgtgcag gctggagaag
     5821 ccgcccgaaa cggcgatgac gcgcgcctga cggatgaaat tcatgacctg aagcgcctgc
     5881 tccagccgcg cgggcgcgac ctccgcgtcc agatccggcg tctcgcgctt caggcggccg
     5941 tgtacgcgct cgctgcgcat cggcaggatc tgcgccagtt gcttgtccgc cccgcccatc
     6001 acctgctcga ccagagcggc gacctccttg tgcgccgccg ccacgcgccg cccccacggc
     6061 ccgtcgcgcg ccagctcgac ataatgctcg aaggattgca actgcgccaa ggcctgcgcg
     6121 acgtgctgcg ccgccagccc catatcctcg cacgggcgcg tgcgcgtgcc catctcctgc
     6181 cgcagcgccg cgacgcgcgc ctcgatatgc gccaggatgc gctcgccgaa taccgccagt
     6241 tccgattcgg ccagaaaacg gtccttgggc cggtcggcca cggtcgccat gaacttgatg
     6301 atctggccgc cgtcctccag attggcgaag atcagctcca gaaagcggca gccgctctcg
     6361 tcctcgaatt cggcggcgtc cttgaacagg acgcgcagcg ccgccgcctt ctccgcgtcg
     6421 atgcggccca tgaattcggg catccggctc agggtctggc ggatgatgcg gtgcaggtcg
     6481 aaataccgcg ccagttcgac ggcctcgttg cgcgcctccg gcgcgctgcc ggcgacggcc
     6541 tccggttcgg cgcggcacag gtcggcgccc gccttggtca gacggaaaaa caccaccggc
     6601 gtcggatcct ccggccgcag cgcgcgtaag gtcgcgaacg cctcgcggaa caggtccgcc
     6661 tcgcgcgccg ccagcccgtt ccagatattc gacaaaaagc ggcgcggaaa gcggacgccc
     6721 tccagcccgt cccggcgcgc ctcgaacagc ggcaggaacg gctgaaacac cgcgtcgcgc
     6781 acctgacggc tctccatctc gacggcgatc aggtcgcgca ccccggccag cgcggcgtcc
     6841 ttgctcaggc ccagcgccgt ctccagcgcg cgcagcgacg ccgcaggcag gctgcggatc
     6901 agcccctgca acagggtcag gcggtgcggg tcgatcgttc tgccgggcgc agccatgcag
     6961 cgaaccagtc ttgtcgtagt cggatgcggg gaaggccagt gtggatggaa aacggttaac
     7021 aatccctgag tggtaaacgg ggaccagcaa gcgctctaag acgccgtccg tccgcccggc
     7081 agatgcgccg tcaccttcag cccgccccag ccggagcgcc cgaagctcag cgtcccgcca
     7141 taggcgcgca ccagctcatc gacgatcgac agaccaagcc ccgaccccgg cacgctttcg
     7201 tccagccgcg ccccgcgctt caacacctcg tcgaagcgcg attccgccag tcccggcccg
     7261 tcgtcctcga ccgacaggct gacggtctgt tcggcggcgt cgaattcggc gtggatcatc
     7321 accttgcgcc ggcaccagat gcaggcgttc tccatcaggt tgcccgcgat ctcctgcaaa
     7381 tcctgccgct cgccgcgaaa ggccagatcg tcgggcgcgc gccagtcgac gaccacgccc
     7441 ttgtcctgaa acacctgctc cagcatgacg gccagttcgt ccagcaccgg ctcgaccggc
     7501 gtgcgctcgc ccatcgcctt ggagcgcgcg gcggcgcggg cgcggcgcag atggtggtcg
     7561 acctgagcgc gcatcgtctc ggcctgcttg cgcacggtct gggccagcgc gtccccggaa
     7621 tcgcccgccg aggtcatcag caccgacaag ggcgtcttga gcgcatgcgc caggttgccg
     7681 acgtgcatgc gctgacgctc gaccacctcc tgcgcgtgat cgagaaaggc gttgagctga
     7741 tgcgcgaccg ggcggatttc cgccggatag gtcttggtca ggcgctgctg ctcgcccgac
     7801 tggacgtgcg cgatctcgtc ggtcaggtcg aacaagggcc gcaaccccac gcgcacctgg
     7861 atgaagatcg ccgccagcgc gcccagcgcc agcaccagca gcgccgccgc cgtcaaaagc
     7921 gcgaaggtgc gcacatcgtg ctcgaccggg ctggaatcct cgcccgccat gaagacatag
     7981 ggcttgttat tgacccgcga atagatgacc gccacgcgca aaggctcgtt caccggtccc
     8041 atgccgtcgt agaacacgct gctgccgcta tgggcgcgcg cgtcgcgcag cacgccggga
     8101 ggcagcggca actgttcgtc ccacagggag cgcgacgtga ccgcactgac gatggcgccg
     8161 ttcgcgccaa cctcgccgac ctgccagtac agcccggaat gcacgatggc cgagcgcgcg
     8221 tcgacgaagc ggctggtgtc caccccgtcg gcgtcgatat tggtgaccga gaacaggctg
     8281 tcggcgatct ggccgacgcg ctcctgaaag cggtgcatgg tgacttcgtg gaagtaggcg
     8341 gtcagcgcga tgccggtgac gatcagcgcg acggtgaaat aggtcgaggc cagccggatc
     8401 agccgtccga ccagcgaatc gcgcgcgctc agtatgcgca gcagccggcg ccagaagcgg
     8461 cgcgggtcga gaccccggcg cggcgcggac tccgccgcgc tcacaggctg ccgacctcgt
     8521 catgcagcgg cttgaggcgg taaccgagac cgcgcacggt ttcgatccgg tcgccgccga
     8581 tcttcttgcg caagcggccc atgaacactt cgatggtgtt ggaatcgcgg tcgaaatcct
     8641 gatcgtagag atgctcgacc agttcggtgc gcgaaatcac ccggttctga tgcagcatca
     8701 gatagtgcaa aagccggtat tccagcgagg tcagccgcaa aggctcgcca ttgaccgagg
     8761 cgcgcgccgc gcgcgggtcc agccgcagcc ccccgcaggt cagcatgggc tgggcatggc
     8821 ccgcggcgcg gcgcaccagc gcgcgcaggc gcgccagcaa ttcctcggtg tggaacggct
     8881 tggtcaggta atcgtcggcc ccggcgtcga aacccgacac cttgtcgctc caggcgccgc
     8941 gcgccgtcag gatcagcacc ggcgtcgtct tgccgtcgcg gcgccagcgc tccagcaccg
     9001 acacgccgtc gatcttgggc aggccgaggt cgaggatcac gcagtcgtaa ggctcggtgt
     9061 cgccgagaaa gtgcgcctcc tcgccgtcgg gcgcatgatc gacgacatag cccgcgtcgg
     9121 acagcgaggt cttgagctga cgcgccagat cgggatcgtc ctcagccagc agaatacgca
     9181 ctatctttct cccacaaccc ggccggtctg gccgtccacc agcagatcga cgcggccgtg
     9241 cccggtgtcg acgcgcaccc agaaggtcga gccgtcctgc tggcccgcgt ccagtacgcg
     9301 cccgcgcgcc cggccggcgg cgatggcgcg gttgatgcgg tcgtcgcggc ggttctcgtc
     9361 gggctgcggt cggtaggccg gttgctgcgg ctgacgctga tcgtcgtcat agcgcggacg
     9421 cggcgcgggc gggcggcggt cgtcgtcatt gctcccccgg cggcgatgct gatccgccgc
     9481 atcgtaatag ggcgcggcct gcacacagga cgcccatgcg gcggccagcg gggccgccgt
     9541 gagcatgagg ctgagaagga gcgcggaacg tttcataggc caaggtataa gctttgacac
     9601 ctgaacctta gctgaacgaa gtcgccgctc ttgtcaaatt cagggaagga tgcgtgcgat
     9661 cccggcctat ttgcgccgcg acggcgtata accgcccttg ccgcgccatt tttcggccag
     9721 cgccgtcagg tcggcctgaa tgtcggtggg aacgccgtcc gccttgtccg gcagcgcgat
     9781 cacgacgtgc gcgaacagat cgccgcgcgc cgcgcccttg ccgtcatagg cgccgcgccc
     9841 tttcaggcgc atgatggcgc ccgaattggt ccccttggcg atgctcaggg acaccggccc
     9901 gtccggcgtc ggagcctgca ccttgccgcc cagcaccgcg tcgggcagcg acacgtagag
     9961 gtccatatgc aggtcatggc cctccagacg gaagaccgga tgcggcttga gatgcagctc
    10021 gaccagcgcg tcgccgtgcg gtccgcgccc gttcggcgac ggcgcgccct gccccttgag
    10081 gcgcagggtc tggccgtcct tcgcgccctt cggaatattg acgtcgacgg tgcgcccgtc
    10141 cgacaaaagc acgcggcggg tgttgccgac aatcgtatcc agaaggtcga tatccagctt
    10201 caggcgcagg tcgccgccct tctgcggcgc cgcgccgaag ccgccgaaac cgccgcccgc
    10261 gccgcccagg ctgaagccga catcggtctt ggagccatgg ccttcttcca tcgccacctg
    10321 ttcgctggtg cggacattgg tgatcgacag cacgacgtcg gcggtcttgg agttgatcga
    10381 gatgccgctg accagcatgc cgacgccgcc gccgatgaag ccgcccagca ggccgccgat
    10441 attggtgcct ccggaattgc cgttcttgga gacgatgtcg ggcaccagga tgtaatcggc
    10501 cgcgcgcacc tggccgccgc cgatattggc gccgccctgc agcgcgtcgc cggccgccaa
    10561 agcgcgttcc tgttgcgcca tctcgaatcc cttgccgcga tcgaccaggg tgaagcagcc
    10621 ggaacgctgc acgaatatct tgagcagcgc ctcgggcgat tccaggccca ggccttccca
    10681 ccagcgattg tcgggctcat ggatggccag cgtgccgatg cggtggctgc aggtggggat
    10741 ttcggctttc ttctcttcgg tcttgcggcg gatcgacgat tgcgcgtagc tcggcgtcac
    10801 caacgcggcc gacgccagac acagcgcggc ggtcatggcg acggattttc ccagacgcat
    10861 ggatgcaccc tctcaaatgg tttcagacag caattgcccc cgcgcggtca gtt
//
LOCUS       DASVLL010000125         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_221640,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000125 DASVLL010000000
VERSION     DASVLL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221640"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VF402_02085"
     CDS             <1..770
                     /locus_tag="VF402_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799109.1"
                     /translation="SMQVGSIVGPALGGMLIAISTQAAYVVCAVMLIVATALIFAIRA
                     DTRPVGVTGSKTEMVKEGAEFIWKSKMLLGAMSLDLAAVLLGGATALIPVFSRDVLHV
                     SPFYFGLMQAATPAGAILMSMSLSQWPIRRRAGPWMYACVAIFGLATIVFGLSRNIWL
                     SIAALAVLGAADMVSVFVRQTLVQIVTPDHMRGRVSSASFLFIGASNQLGEFESGTVA
                     RFLGPVGAALFGGFGSLLVTLVWIKLFPVLFKADRLE"
     gene            complement(771..2252)
                     /locus_tag="VF402_02090"
     CDS             complement(771..2252)
                     /locus_tag="VF402_02090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7799110.1"
                     /translation="MAQLDPRRQPVRLSPMLFASAAAITAGAIMWRLLQPLSGLHPPM
                     DPAASMALETQAFSEAAARPGYSPAVDVPISIRSGETLADAVARMGVAPAEAKAAVTL
                     MSQAFNLQNLKAGLSLEAAIARPANDADAPAQLLGLTLRTGPAKQLTLTTARDGSMRL
                     RALEESVRDERRVAIGPVSGSLFTSAAALGATPSLTAQVVKLFAHKLDFERDIQPGDT
                     FKLVFDRKVTESGRTVEAGNLLYAEIETRAGADRFYAFQPKGSGEIEYFDENGKNIKG
                     FLLTTPVYGARISSTFGRRSDPFLGFTKMHTGIDFAAPLGTPIMAAGDGVVEDAKWWG
                     GYGRWVRIRHNAEWETGYGHMSAIAVKPGQHVTQGQIIGYVGTTGRSTGPHLHFEVWQ
                     NGHPINPRDARVPDSNVLQGQDMIAFRARKRAIDGMLVMASSHTDGGSGAVAINTGDY
                     HFEGEDATSRPDSTTLARNEDGALRVNSLAPLRSALTTTRGMR"
BASE COUNT          373 a          851 c          942 g          466 t
ORIGIN      
        1 tgtcgatgca ggtcggctcc atcgtcggtc cggcgctggg cgggatgctg atcgcgatct
       61 cgacccaggc cgcctatgtg gtgtgcgcgg tcatgctgat cgtggcgacc gcgctgatct
      121 tcgccatccg cgctgacacc cggcccgtcg gcgtcaccgg ctccaagacc gagatggtga
      181 aggagggggc ggaattcatc tggaaatcga agatgctgct gggcgcgatg tcgctggatc
      241 tggcggcggt gctgctgggc ggcgcgacgg cgctgatccc ggtgttctcg cgtgacgtgc
      301 tgcacgtctc gcccttctat ttcgggctga tgcaggcggc gacgcccgcg ggcgccatcc
      361 tgatgtccat gtcgctgtcg caatggccga tccgccgccg ggccggaccg tggatgtacg
      421 cctgcgtggc gatcttcggg ctggcgacca tcgtgttcgg cctgtcgcgc aatatctggc
      481 tgtcgatcgc ggctctggcc gtgctgggcg ccgccgacat ggtcagcgtc ttcgtgcgcc
      541 agaccctggt gcagatcgtc acgcccgacc acatgcgcgg gcgcgtgtcg tcggcctcgt
      601 tcctgttcat cggcgcctcg aaccagcttg gcgagttcga gagcggcacg gtggcgcggt
      661 tcctggggcc ggtcggggcg gcgctgttcg gcggcttcgg ttcgctgctg gtgacgctgg
      721 tctggatcaa gctgttcccg gtgctgttca aggccgaccg gctggagtga tcagcgcatg
      781 ccgcgcgttg tggtcagggc cgagcgcagc ggcgccaggc tgttgacgcg cagcgcgccg
      841 tcctcgttcc gggccagggt ggtcgagtcg ggtcgcgacg tcgcgtcctc gccctcgaaa
      901 tgatagtcgc cggtgttgat cgcgacggcg ccggaaccgc cgtcggtatg gctggacgcc
      961 atcaccagca tgccgtcgat ggcgcgcttg cgggcgcgga aggcgatcat gtcctgcccc
     1021 tgcaacacgt tggagtcggg cacgcgcgcg tcgcgcggat tgatcggatg gccgttctgc
     1081 cagacctcga aatgcaggtg cggcccggtg gagcgccccg tggtgccgac atagccgatg
     1141 atctggccct gcgtgacatg ctggcccggc ttgaccgcga tggccgacat gtggccgtag
     1201 ccggtctccc actcggcgtt gtgccggatg cgcacccagc gcccgtagcc gccccaccat
     1261 ttggcgtcct cgaccacgcc gtcgcccgcg gccatgatcg gcgtgcccag cggcgccgcg
     1321 aagtcgatgc cggtgtgcat cttggtgaag ccgaggaacg ggtcggaacg ccgcccgaag
     1381 gtcgaggaaa tacgcgcgcc atagaccggc gtggtcagca ggaagccttt gatgttcttg
     1441 ccgttctcat cgaaatactc gatctcgccg ctgcccttgg gctggaaggc gtaaaagcgg
     1501 tcggcgccgg cgcgcgtctc gatctcggca tagagcaggt tgcccgcctc gaccgtgcgg
     1561 ccgctttcgg tcaccttgcg gtcgaacacc agcttgaagg tgtcgccggg ctggatgtcg
     1621 cgctcgaagt ccagcttgtg cgcgaacagc ttgaccacct gcgcggtcag ggacggcgtc
     1681 gcgcccagcg cggcggcgga ggtgaacagc gagccggaca ccgggccgat ggccacgcgg
     1741 cgctcgtcgc gcaccgattc ctccagcgcg cgcagccgca tcgagccgtc gcgcgccgtg
     1801 gtcagggtca gttgcttggc cgggccggtg cgcaaggtca ggcccagcag ttgcgcgggc
     1861 gcgtcggcgt cgttggccgg gcgcgcgatg gcggcctcca ggctcaggcc cgccttgagg
     1921 ttttgcaggt tgaaggcctg cgacatcagc gtgaccgccg ccttggcctc ggcgggcgcg
     1981 acgcccatgc gcgccaccgc gtcggccagc gtctcgcccg agcggatcga gatcggcacg
     2041 tcgacggcgg ggctgtagcc ggggcgcgcg gcggcttcgg aaaaggcctg cgtctccagc
     2101 gccatgctgg ccgccgggtc catcggcggg tgcaggccgg acaggggctg caacaggcgc
     2161 cacatgatcg ctcccgccgt gatcgccgcc gccgacgcga acagcatcgg cgacaatctc
     2221 acgggctgac ggcggggatc gagttgagcc atgtcggcgg cgatgtcctt tgcgcggggc
     2281 cggttcgcgg ccggcgtgaa caggcgcgca gtatggcctg agtcgaaaaa actgtggata
     2341 gctttccata agctaaaaaa gggcgaaaat gaggcgcgtt tacgcctgcg gcgcgagcgc
     2401 gcagcggccg gaaggacgcc gtaacgccgc ctttcatgtc ggtcgccgcg aattcccctg
     2461 tcgcgggaat cgttcacgaa gccttaacat tctgtggaaa actcgccgcc gcccccggcg
     2521 cggcggtcat cttttcgccg cgcggacgga aaacagtggc cgcgaaccgg gcgcgtccgt
     2581 attttcttta ccggtttgga acatggtaaa ttcagaacgc agaaagctct at
//
LOCUS       DASVLL010000126         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_114491,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000126 DASVLL010000000
VERSION     DASVLL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114491"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VF402_02095"
     CDS             complement(<1..295)
                     /locus_tag="VF402_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799111.1"
                     /translation="MAMQTDKRWLWLAGGAVAALAIGVALAVGLSSGHKADKPMNDQP
                     TGLTFNVSDPKPTFDPKKPLRCYVAGAYVGDLTLADCARKNGVAAQALDVGQDD"
     gene            complement(429..>2408)
                     /locus_tag="VF402_02100"
     CDS             complement(429..>2408)
                     /locus_tag="VF402_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7799112.1"
                     /translation="PASQPGDPISKVGAKYWVSGEAGQTALPDSVVNVRATASGPDGS
                     VYVLADVNDATGGQLISGGQDVALMKYDSAGKLVYTRALGAADTASGFALSVSADGKV
                     AVAGSVTGALETAASKTTTYGSGKTAVSFTSTTETGLRGLDDSISDSFVTVLDADGVE
                     MWTQRRGGSGADEATSVAFGDDGSVYVGGRTQSLMPGASGGARGGWDGYVMGFTDKGA
                     VKFTLQTGGSGSDRVSQLAVDGDTLYVAGVNDNQANLTSYRLGSYETTDDKGNPLTKY
                     TAVQTASRDLGGMGGGTISGMAVENGQIYLGGSSSNGGLLGGASVTRAWSGGQDAYAL
                     KVSEDLGDTSADVIAFYGGAGKEADARVQFVGGKAWIAGATDGEIDGVARLGTGEKAQ
                     DGYIARLNLDTGAVEYQQRYTGTDNKVNPGAVTVVRNGASVLDRLGLPQGTIQQKDSD
                     LVIAGTSVRTGDQFYLVDPASGARKTVTIEAVDTLKTLADKIERASGHKLSVDVSKLL
                     GKPVDQLVIKPANAASKMEILAGPAGKDALKGLGIDPGLISNDATKIMDASSSGYLKS
                     RKPIGLNFDASLNLNSDANIQKALDGLQAVMKTVQRAYTYLKYGDPQAADSDKHGKTG
                     GTVPTYLTNQISNYQAALNRLTGGAPASKTGLFGL"
BASE COUNT          325 a          858 c          783 g          442 t
ORIGIN      
        1 cgtcgtcctg cccgacgtcc agcgcctgcg ccgcgacgcc gttcttcctg gcgcagtcgg
       61 ccaaggtcag gtcgccgaca taggccccgg cgacatagca gcgcagcggt tttttcggat
      121 cgaaggtcgg cttgggatcg ctgacgttga aggtcaggcc ggtcggctga tcgttcatcg
      181 gcttgtcggc cttgtgtccc gacgacagcc ccaccgccag cgcgacgccg atcgccaaag
      241 ccgccacggc tccgcccgcg agccacagcc agcgtttatc ggtctgcatc gccatcggcg
      301 tcccccttgt ttcggctcaa cgcgccgtga attagagtcg tgtctgcgcg cgatcatagc
      361 agaggcgcga ggcaggccaa gccggaaagc gcgccttcgc tataggcggg cgccgtgacg
      421 aaaaaatgtc agaggccgaa cagcccggtt ttcgacgcgg gcgcgccgcc ggtcaggcgg
      481 ttcagcgccg cctgatagtt ggagatctgg ttggtcagat aggtcgggac cgtgccgccg
      541 gtcttgccgt gcttgtccga atcggcggcc tgcggatcgc cgtatttcag gtaggtgtag
      601 gcgcgctgca cggtcttcat caccgcctgc aagccgtcga gcgccttctg gatgttggcg
      661 tcgctgttca ggttcaggct ggcgtcgaaa ttaagcccga tgggcttgcg cgatttcaga
      721 tagccgctcg acgaggcgtc catgatcttg gtcgcgtcgt tgctgatcag gcccggatcg
      781 atccccagtc ctttcagcgc gtccttgccc gccggtccgg ccaggatctc catcttgctg
      841 gccgcgttgg ccggcttgat caccagttgg tcgaccggct tgcccagcag cttgctcaca
      901 tcgacgctga gcttgtggcc ggaggcgcgc tcgatcttgt cggccagggt tttcagcgtg
      961 tccaccgcct cgatggtgac ggtcttgcgc gcgccggacg ccggatcgac cagatagaac
     1021 tgatcgcccg tgcgcacgct ggtcccggcg atgacgaggt cggaatcctt ctgctggatc
     1081 gtcccctgcg gcaggcccag ccggtccagc acgctggcgc cgttgcgcac gaccgtgacc
     1141 gcgcccggat tgaccttgtt gtccgtcccg gtatagcgct gctggtattc gaccgcgccg
     1201 gtgtcgagat tgagccgggc gatatagccg tcctgcgcct tttcgcccgt gccgagccgc
     1261 gccacgccgt cgatctcgcc gtcggtggcg ccggcaatcc acgccttgcc gccgacgaac
     1321 tgcaccctgg cgtcggcctc cttgccggcg ccgccgtaga aggcgatgac gtcggcgctc
     1381 gtatcgccga ggtcttcgct gaccttcagc gcgtaggcgt cctgcccgcc cgaccaggcg
     1441 cgcgtcacgc tggcgccgcc gagcaggccg ccgttgctgc tggagccgcc cagatagatc
     1501 tggccgttct cgaccgccat gccgctgatc gtcccgccgc ccatgccgcc cagatcgcgg
     1561 ctcgccgtct ggaccgcggt gtatttggtg agcggattgc ccttgtcgtc ggtcgtttcg
     1621 tagctgccga gccggtacga ggtcaggttc gcctgattgt cgttgacccc ggcgacgtac
     1681 agcgtgtcgc cgtcgaccgc cagttgcgac acccggtcgg aacccgaccc gccggtctgg
     1741 agcgtgaact tgaccgcgcc cttgtcggtg aagcccatga catagccgtc ccagccgccc
     1801 ctggcgccgc cggacgcgcc gggcatcagc gactgcgtgc gcccgccgac atagaccgag
     1861 ccgtcgtcgc cgaacgccac cgaggtcgcc tcgtccgccc cggagccgcc gcgccgctgg
     1921 gtccacatct cgaccccgtc ggcgtccagc accgtcacga agctgtcgct gatcgagtcg
     1981 tccagccccc tgaggccggt ttcggtcgtc gacgtgaagc tcacggcggt cttgccggag
     2041 ccgtaggtcg tggtcttgct cgccgccgtc tccagcgcgc cggtcaccga accggcgacc
     2101 gcgaccttgc cgtccgccga cacgctcagc gcgaagcccg acgccgtgtc cgccgcgccc
     2161 agcgcgcggg tatagacgag cttgcctgcc gaatcgtact tcatcagcgc cacgtcctgc
     2221 ccgccgctga tcaactggcc gcccgtggcg tcgttgacgt cggccagcac atagaccgag
     2281 ccgtccggcc ccgaggccgt ggcgcgcaca ttgaccaccg aatcgggcaa ggcggtctgg
     2341 cccgcctcgc cgctgaccca gtatttggcg ccgaccttcg agatcgggtc gcccggctgg
     2401 ctggcggg
//
LOCUS       DASVLL010000127         3624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_491032,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000127 DASVLL010000000
VERSION     DASVLL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491032"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VF402_02105"
     CDS             complement(<1..238)
                     /locus_tag="VF402_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX7799113.1"
                     /translation="MKKIPTAIFISGRGSNMMALVEAAKAEDFPAEFVLVVANDPDAP
                     GLQWAAERGIRTFALDHKPFGKDREAHERAIDAAL"
     gene            complement(278..1321)
                     /gene="purM"
                     /locus_tag="VF402_02110"
     CDS             complement(278..1321)
                     /gene="purM"
                     /locus_tag="VF402_02110"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX7799114.1"
                     /translation="MSEPSGAPTSSNGLTYAQSGVDIDAGEALVDAIKPLAKATRRPG
                     AEASLGGFGALFDLRAAGYDDPLIVTTTDGVGTKLKIAIETHRHDTVGIDLVAMCVND
                     LLAQGAEPLMFLDYYATSKLDVDTARRVVAGIAEGCKRADCALVGGETAEMPGMYGDS
                     DYDLAGFSVGAVDRNKVLPRFDDQRAGDVIIGLASSGPHSNGYSLIRKVIEVAGLSWA
                     SEAPFARDMSLAQALLEPTRIYIKSVLPLMKAGLIKGGAHITGGGLIENPDRGVAEHL
                     TARFDFDGWAFPPVFQWLMEAGNIDRMEMLRTFNCGVGFVLYVAPKDADAVLAALLNA
                     GEDAFVCGQLAAR"
     gene            1427..2575
                     /gene="rnd"
                     /locus_tag="VF402_02115"
     CDS             1427..2575
                     /gene="rnd"
                     /locus_tag="VF402_02115"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX7799115.1"
                     /translation="MTPITNTNDLIAFCEAIKSAPFITVDTEFMRETTYWPKLCLIQA
                     ASEDHAAIIDPLAADLDMKPFLDILADEKVLKVFHACRQDMEIFVNLGVLPKPVFDTQ
                     VAAMAAGYGDQVAYDSLVRQKLKIDIDKGSRFTDWARRPLSEQQLKYALGDVTHLAKL
                     YPKLRDRLAKQGRLDWVAEEMIALTDPALYATDPNEAWRRLRPRKTSAKYMAAFAQVA
                     AWRERTAQERDMPRGRILKDEGVDEIATQLPTDSAAFDHLRSTSKGFGSSKFGLELAD
                     ILRHVLKDPEAYAPKVEKSGPPAQVPASVVELLKVLLRIRCEEEGVAPKLLATVADLE
                     KIALDDKAKVPALEGWRRQVFGEDALRLKHGELALVLNGTKVELVELE"
     gene            complement(2594..>3624)
                     /locus_tag="VF402_02120"
     CDS             complement(2594..>3624)
                     /locus_tag="VF402_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEX7799116.1"
                     /translation="MAGVTLPLGPFALGAPKPIDVAKAAEQIREQLQPLQGRFVNPHF
                     HAVGGAWRNLALIWMMKQHYPLQIVQQFELPAREALSIARLIASQSLSSLEKLPGVSK
                     RRIENISYAALVLQGLIEELKFETLCFSAFGLREGLLFDAMPEAMRRDDPLIAGCAAL
                     GARLGINDELGVALADWALAFYAGCAPDLTPPSEKLVRAAAKLSDIGARLHPDHRADL
                     ASDQVLRAPIPGQNHEERVFLACALYTRYSGDPYTREPVLTGRILGEDGLAQATRLGL
                     LLRLGCDLSAKSARLLKRSHIRQDGEHLTLSAKTGWEDLLMGEQTRRRAKALAHALKL
                     NLNIISYN"
BASE COUNT          650 a         1178 c         1187 g          609 t
ORIGIN      
        1 gcagggccgc gtcgatggcg cgctcatgcg cctcccggtc cttgccgaac ggcttgtgat
       61 cgagcgcgaa cgtcctgatg ccgcgctccg ccgcccattg caggcccggc gcgtcaggat
      121 cgttggcgac caccagcacg aactcggcgg gaaaatcctc ggccttggcg gcctcgacca
      181 gcgccatcat gttcgagccg cgaccggaga taaagatggc ggtaggtatt tttttcattg
      241 cgctaacgct tcgcttcctg agcgcgggtc ttggacatta acgcgccgcc agttgaccgc
      301 agacgaaggc gtcctcgccc gcgttcagaa gcgccgccag caccgcgtcg gcgtccttcg
      361 gcgcgacata cagcacgaag ccgacgccgc agttgaaggt gcgcagcatc tccatcctgt
      421 cgatattccc ggcctccatc agccactgga acaccggcgg gaacgcccag ccgtcgaaat
      481 cgaaccgcgc cgtcaggtgt tcggccacgc cgcgatccgg gttttcgatc agcccgccgc
      541 cggtgatatg ggcgccgccc ttgatcaggc ccgccttcat cagcggcagc accgacttga
      601 tatagatgcg cgtcggctcc agcagcgcct gcgccagcga catgtcgcgc gcgaacggcg
      661 cctcggacgc ccaggacagg cccgccacct cgatcacctt gcggatcagc gaatagccgt
      721 tggagtgcgg ccccgacgag gcgaggccga tgatcacgtc gcccgcgcgc tgatcgtcga
      781 agcgcggcaa aaccttgttg cggtccacgg cgccgaccga aaaaccggcc agatcgtaat
      841 cgctgtcgcc atacatgccc ggcatttccg ccgtctcgcc gccgaccagc gcgcagtccg
      901 cgcgcttgca gccctcggcg atgcccgcca ccacgcggcg cgcggtgtcc acgtccagct
      961 tcgaggtggc gtaatagtcg aggaacatca acggctcggc gccctgcgcc agcaggtcgt
     1021 tgacgcacat ggccaccagg tcgatgccga ccgtgtcatg gcggtgcgtc tcgatggcga
     1081 ttttcagctt ggtgccgacg ccgtcggtcg tggtcacgat cagcggatcg tcgtaaccgg
     1141 ccgcgcgcag atcgaacagg gcgccgaagc cgccgaggct ggcctcggcg cccggacggc
     1201 gcgtcgcctt ggccagcggc ttgatcgcgt ccaccaacgc ttcgcccgcg tcgatgtcga
     1261 cgccggactg cgcataggtg aggccgttgg aggaggtcgg ggcgccgctg ggttcagaca
     1321 tttctcttcc ttgcgctaac gcttcgctgc ttgagcgcgg tataaatcga atctcgatat
     1381 gacatttaag actttaaagc cgaagcgcgt ttgctatagc tacaaaatga cgccaattac
     1441 aaatacgaat gatctgatcg ccttctgtga agcgataaaa tccgcgccct tcattacggt
     1501 cgacaccgaa ttcatgcgcg agacgaccta ctggcccaag ctgtgtctga ttcaggccgc
     1561 cagcgaggac cacgccgcga ttatcgatcc gctggccgcc gatctcgaca tgaagccgtt
     1621 tctcgatatc ctggccgatg aaaaggtgct gaaagtcttt cacgcctgcc gtcaggacat
     1681 ggagatcttc gtcaatctcg gcgtgctgcc caagcccgtg ttcgacacgc aggtggcggc
     1741 gatggcggcg ggttacggcg atcaggtcgc ctacgattcg ctggtgcggc aaaagctcaa
     1801 gatcgacatc gacaaaggct cgcgcttcac cgactgggcg cgccgtccgc tcagcgagca
     1861 gcagttgaaa tacgcgctgg gcgacgtgac ccatctggcg aagctgtacc ccaagctgcg
     1921 cgaccggctg gcgaagcagg gacggctgga ctgggtggcc gaggagatga tcgccctgac
     1981 cgatccggcg ctctacgcca ccgacccgaa cgaagcgtgg cggcgtttgc ggccgcgcaa
     2041 gacctcggcc aagtacatgg ccgccttcgc gcaggtggcg gcgtggcgcg aacgcacggc
     2101 gcaggagcgc gacatgccgc gcggccgcat cctgaaagac gagggcgtcg acgagatcgc
     2161 cacgcagttg ccgaccgatt cggcggcctt cgaccatctg cgctcgacct ccaaggggtt
     2221 cggatcgtcc aagttcgggc tggagctggc cgacatcctg cgccatgtgc tgaaagaccc
     2281 ggaagcctat gcgcccaagg tcgagaagtc cggcccgccc gcgcaggtgc ccgcttcggt
     2341 cgtggagttg ctgaaggtgc tgctgcgcat ccgctgcgag gaggagggcg tggcgccgaa
     2401 actgttggcg accgtggccg atctggaaaa gatcgcgctg gacgacaagg cgaaggtgcc
     2461 cgcgctggaa ggctggcgcc gtcaggtgtt cggcgaggac gcgctgcggc tcaagcacgg
     2521 cgaactggcg ctggtgctga acggaaccaa ggtcgagctg gttgagctgg aatagaaaaa
     2581 gcgctgcttt ttcttaatta tagctgataa tattcagatt tagcttgagc gcgtgcgcca
     2641 gcgccttagc gcggcggcgc gtctgttcgc ccatcagcag gtcttcccag ccggtcttgg
     2701 cgctcagcgt cagatgctcg ccgtcctgac ggatgtgcga acgtttcagc aggcgcgccg
     2761 acttggccga caggtcgcag ccgaggcgca gcagcaggcc gaggcgcgtc gcctgcgcca
     2821 aaccgtcctc gccgaggatg cgcccggtca gcaccggttc gcgcgtatag ggatcgccgg
     2881 aatagcgggt gtagagcgcg caggccagaa acacgcgctc ctcatggttc tggcccggaa
     2941 tcggcgcgcg cagcacctga tcgctggcca gatcggcgcg gtggtcggga tgcaggcggg
     3001 cgccgatgtc gctgagtttg gcggcggcgc gcaccagctt ttccgacggc ggcgtcaggt
     3061 ccggcgcgca gcccgcgtaa aaggccagcg cccagtcggc cagcgcgacg cccagttcgt
     3121 cattgatgcc gagccgcgcg cccaacgccg cgcaaccggc gatcagggga tcgtcgcggc
     3181 gcatcgcttc gggcatggcg tcgaacaaaa gcccttcgcg caggccgaag gccgagaagc
     3241 acagcgtctc gaacttcagt tcctcgatca gcccttgcag caccagcgcg gcgtaggaaa
     3301 tgttctcgat gcggcgtttg gagacgccgg gcagcttttc cagcgacgac agcgactggc
     3361 tggcgatcag gcgcgcgatg gacagggctt cgcgcgcggg cagctcgaac tgttgcacga
     3421 tctgcaacgg ataatgctgc ttcatcatcc agatcagcgc cagattgcgc cacgcgccgc
     3481 cgacggcgtg gaaatgcggg ttgacgaaac ggccctgcaa aggctgaagc tgctcgcgaa
     3541 tctgctccgc cgctttggcg acgtcgatcg gcttgggggc gccgagcgcg aacggcccca
     3601 gcggcagggt gacgcccgcc atac
//
LOCUS       DASVLL010000128         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_491053,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000128 DASVLL010000000
VERSION     DASVLL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491053"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1915)
                     /locus_tag="VF402_02125"
     CDS             complement(<1..1915)
                     /locus_tag="VF402_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799117.1"
                     /translation="MKFKRHILMTSAVASLGFALSLGAAATAAHAQTAAAAAPSDDST
                     TVVVTGIRKSLQDALRQKRSSDRVEEVITAEDIGKFPDKNVADSLSRATGVNVVTGTS
                     AGGGFGENEHISIRGTDPNLNLTLLDGHNMATGDWFVLDQTAGGRAFNYTMLPSEVVG
                     TINVIKSSSADLPEGGMGGTVDVHVRKPLDLPANTGAFSVEALYSTRPDKTTPQVSGM
                     YSWKNGAHTFGVLVGAFYEQRDFRRDGQEVLGYTTVTNWAGTGQNLNVPSLIGAAYFT
                     QQRTREGGNFVAQWKPSDKLELDFSGLYTHLEANNENHNFMMWGSKLLTANSDHYITD
                     PNDSSKQILVKALGANVPGSLLTGYTVSGDYVTSASWKADPSDSTGTIVQDDIFRQAH
                     SDSYSLNLDGAWQATDKLNVKGQIGFTAGEGDTDDSAAWETFWSGTGASYTLGNPTAK
                     VSYPGLNKDPTSAAYLNNQWSWSWGGAVKSPDREAYGKIDADYSVDAGALRDIAFGAR
                     YTDHTRRLDDIAYSWAGNGAGGVNLATVYKGGITPDNYGSDIGGLPTYTFANGQAVYN
                     YEDTHNGGRVFGFYAPASFAVHEKTDAYYVMAKLGGDRWNGNIGVRAVHTNEISTQYT
                     TNVTPDMTTTPSVF"
BASE COUNT          365 a          661 c          712 g          441 t
ORIGIN      
        1 cgaacaccga aggcgtcgtc gtcatgtcgg gcgtgacgtt ggtggtgtac tgcgtcgaga
       61 tctcgttggt atgcaccgcg cgcacgccga tattgccgtt ccagcggtcg ccgcccagct
      121 tggccatcac gtaataggcg tcggtctttt catgcacggc gaacgaggcc ggggcgtaga
      181 agccgaagac gcggccgccg ttgtgggtgt cttcgtaatt atagaccgcc tgaccgttgg
      241 cgaaggtgta ggtcggcagg ccgccgatat ccgaaccgta attgtccggc gtgatcccgc
      301 ccttatagac ggtggccaga ttgacgccgc ccgcgccgtt gcccgcccag ctataggcga
      361 tgtcgtcgag acggcgggtg tggtcggtgt agcgggcgcc gaaagcgatg tcgcgcagcg
      421 cgccggcgtc gaccgaatag tcggcgtcga tcttgccgta ggcctcgcgg tccggcgact
      481 tgaccgcgcc gccccacgac cagctccact ggttgttcag ataggccgcc gaggtcggat
      541 ccttgttcag gcccggatag gagaccttgg cggtcgggtt gcccagcgta tagctggcgc
      601 ccgtgccgga ccagaaggtt tcccacgccg ccgaatcgtc ggtgtcgcct tcgccggccg
      661 tgaagccgat ctggcccttg acgttcagct tgtcggtcgc ctgccaggcg ccatcgagat
      721 tcaggctgta ggaatcggag tgcgcctgac ggaagatgtc gtcctgcacg atcgtgccgg
      781 tcgaatcgga aggatcggcc ttccaactgg ccgaggtcac atagtcgccc gacaccgtat
      841 agcccgtcag caggctgccc ggcacgttgg cgcccagcgc cttgaccagg atttgcttgc
      901 tcgagtcgtt ggggtcggtg atgtaatgat ccgagttcgc ggtcagcagc ttcgagcccc
      961 acatcatgaa gttgtggttc tcgttattgg cctccaggtg cgtatagaga ccggagaagt
     1021 ccagttcgag cttgtccgaa ggcttccatt gcgcgacgaa attgccgcct tcgcgggtgc
     1081 gctgctgggt gaagtaggcg gcgccgatca gcgacggcac gttcaggttc tgaccggtcc
     1141 cggcccagtt ggtgaccgtg gtgtagccca gcacttcctg accgtcgcgg cggaaatcgc
     1201 gctgctcata gaaggcgccg accagcacgc cgaaggtgtg cgcgccgttc ttccacgaat
     1261 acatgcccga cacctgcggc gtcgtcttgt ccgggcgcgt cgaatagagc gcctcgaccg
     1321 agaaggcccc cgtattggcc ggcaggtcca gcggcttgcg cacatgcacg tcgaccgtgc
     1381 cgcccatgcc gccttcgggc aggtcggcgg acgaggactt gatcacattg atcgtgccga
     1441 ccacttcgga cggcagcatg gtgtagttga aggcccggcc gcccgccgtc tggtccagaa
     1501 cgaaccagtc gccggtcgcc atgttgtggc cgtccagcag ggtcaggttc aggttcggat
     1561 cggtgccgcg gatcgagata tgctcgtttt caccgaagcc gccgcccgcc gaggtgccgg
     1621 tgacgacgtt gacgccggtg gcgcgcgaca gcgagtcggc gacgttcttg tccgggaact
     1681 tgccgatgtc ctcggcggtg atcacttcct cgacgcggtc ggagctgcgc ttctgacgca
     1741 gcgcgtcctg caacgacttg cggatgccgg tgacgacgac cgtggtggaa tcgtcggacg
     1801 gcgcggcggc ggcggcggtc tgcgcgtgcg cggccgtggc ggccgcgccc agcgacagcg
     1861 cgaagccgag gctggcgacc gcgctggtca taagaatgtg gcgtttgaac ttcatcttgg
     1921 tctttcccta ttcgtgccgc ctgcggcgaa ggcgagcccc tgaaacctta tgtctgatcg
     1981 ccgcgtcgat aagtggtgga tcggcggtcc gcatgccagt ctgatacctg tacataccaa
     2041 cctaatacct aagccgggaa aagagctaac tttgacccca ttcaagtgtc aactcaaaac
     2101 tgtcatatct gtaattggtc cttaattgcg ccgaatgcgt gacattaata tcgcagttat
     2161 tgtcatatat tttcgtgaa
//
LOCUS       DASVLL010000129         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_169294,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000129 DASVLL010000000
VERSION     DASVLL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169294"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..414)
                     /locus_tag="VF402_02130"
     CDS             complement(34..414)
                     /locus_tag="VF402_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX7799118.1"
                     /translation="MTLLLNLLWLVFGGFISGCLWLLGGLILACTVVGLPWAFSAWRI
                     AGFVFWPFGRRIVDRPADPVSGCLGVILNIVWFVFAGWYIALAHVLIAIPEAISIVGI
                     PFAFKDLQLAQLALAPVGKEIVDA"
     gene            657..866
                     /locus_tag="VF402_02135"
     CDS             657..866
                     /locus_tag="VF402_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEX7799119.1"
                     /translation="MADLINLNKARKTRARAQKTAEAAQNRVLHGLPGAKRAAARAAR
                     EQEAARLEGHKLTGAVTTKSVDDTQ"
     gene            1012..1251
                     /locus_tag="VF402_02140"
     CDS             1012..1251
                     /locus_tag="VF402_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX7799120.1"
                     /translation="MPGLKKRSVNLAGHATSVALEPEFWAVLEEMSKARKISLAALIA
                     ELDSRRGESLLASFCRLSALAYVQQKADAPRRRKA"
     gene            1322..>2159
                     /locus_tag="VF402_02145"
     CDS             1322..>2159
                     /locus_tag="VF402_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7799121.1"
                     /translation="METSTPAPKVSDLAAARPDFFRGLNPEQEAAVRATQGPVLVLAG
                     AGTGKTRVLTTRIAHILAQGLARPWEILCVTFTNKAAREMRERTVALVGAAGEGLSNL
                     GTFHSIAAQILRRHADLAGLKSSFTILDDDDQVRLIKALLEAENVDTKKWTPKSFAFH
                     LDQWKNKGLTPDKVARSSNVSGSAKQNVDEGAAFANGRGVKFYQMYQDRLRILNACDF
                     GDLLLHNLEILTKHPDVLERYHQRFKYILVDEYQDTNVAQYLWLRLLTNADRNLCCVG
                     DDD"
BASE COUNT          472 a          657 c          683 g          347 t
ORIGIN      
        1 aattaaaagg gagtcatgaa cttgcggaca tcttcaggcg tccacgatct ccttgccgac
       61 cggggccagc gccagttgcg ccagttgcag atccttgaag gcgaacggaa tgccgacgat
      121 ggagatcgct tcggggatgg cgatcagcac gtgcgccagc gcgatgtacc agcccgcgaa
      181 cacgaaccag acgatgttca ggatcacgcc cagacagccg ctgaccggat cggcggggcg
      241 gtcgacgatg cgccgcccga acggccagaa gacgaagccg gcgatgcgcc acgccgaaaa
      301 ggcccacggc agcccgacca ccgtgcaggc cagaatcaaa ccgcccagca gccacaggca
      361 gccggagata aagccgccga aaaccagcca gagaagattg agcaggagag tcatgccgaa
      421 ggctccgcaa taaaaccgcg cgatccggac tgtaaacacg tatcgggcgc cgggccatgt
      481 taaattttgc tcatgtgggc aggatttacc gcgccgcagt tggacgaacg ctgttttttg
      541 aattatgatg caggcgaaag cggagcggcg ccgcaaagcg catcgttatt ttataggcgg
      601 tatgccatga cggtaggctg tcgtattttt cgcatggacg cgggacggga agacgcatgg
      661 cggacctgat caatctcaac aaggcgcgca agacccgcgc ccgcgcgcaa aagacggcgg
      721 aggcggcgca gaaccgcgtc ctgcacggcc tgcccggcgc caaacgcgcc gccgcccgcg
      781 ccgcgcgcga acaggaggcc gcgcggctgg aaggccataa gcttaccggc gcggtaacca
      841 cgaaatcagt tgacgatacg cagtaaaaac cgccagatta ccgccctgtt gaagtaaaat
      901 ggttaaatct taccggggga gcggtagggt ttgaccagta taaatgtggg gggcattgat
      961 ccgaagctaa tcccgggaag gttcgccttc tcaaaagtcg gaggagagca aatgccaggc
     1021 ctgaaaaaac gatcggtgaa tctggccggt cacgccacgt cggtggcgct tgagcccgaa
     1081 ttctgggccg tactggaaga gatgtcgaag gcgcgcaaga tcagcctcgc cgccctgatc
     1141 gcggaactcg attcccgccg gggcgaaagc ctgctggcgt cgttctgccg cctgtcggcg
     1201 ctggcctatg tgcagcaaaa ggccgatgcg ccgcgccgcc gcaaggcgta agtcccggat
     1261 ttctgttcgc gttctgcatt tgtcccttgg cgcggcgggc aagtgggatt aactgaggcg
     1321 catggaaacc tcgacacccg ctcccaaggt cagcgatctg gccgccgcgc gcccggattt
     1381 tttccgcggc ctcaatcccg aacaggaagc cgccgtgcgc gccacgcaag gcccggtgct
     1441 ggtgctggcc ggggcgggca ccggcaagac gcgcgttctg accacgcgca tcgcgcacat
     1501 tctggcgcag ggtctggccc ggccgtggga aatcctgtgc gtgaccttca ccaacaaggc
     1561 ggcgcgcgag atgcgcgaac gcaccgtggc cctggtcggc gcggcggggg aggggctgtc
     1621 gaatctcggc accttccatt ccatcgccgc gcagatcctg cgccgccacg ccgatctggc
     1681 cggcctgaaa tccagcttca cgattctcga cgacgacgat caggtgcggc tgatcaaggc
     1741 gctgctcgaa gccgaaaacg tcgatacgaa gaaatggacg cccaaatcct tcgccttcca
     1801 tctcgaccag tggaagaaca aggggctgac gccggacaag gttgcgcgct caagcaacgt
     1861 aagcggtagc gcaaagcaaa atgttgacga gggcgcggcg tttgcgaacg ggcgcggcgt
     1921 caagttctac cagatgtatc aggaccggct gcgcatcctg aacgcctgcg atttcggcga
     1981 tctgctgctg cacaatctgg agattctgac gaagcatccc gacgtgctgg agcgttatca
     2041 ccagcggttc aaatacatcc tcgtcgacga atatcaggac accaacgtcg cgcagtatct
     2101 gtggctgcgg ctgctgacca acgccgaccg caacctctgc tgcgtcggcg acgacgatc
//
LOCUS       DASVLL010000130         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_60923, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000130 DASVLL010000000
VERSION     DASVLL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60923"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VF402_02150"
     CDS             <1..537
                     /locus_tag="VF402_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX7799122.1"
                     /translation="KNCVAIGLSSGFLEPLESTSINFIQNAAVRLLELFPQKQIDPCL
                     RDEYNRRNQASYDYVRDFIIAHYCLNSRDGDLWRDCARMAIPDDLRWKIELFKRRGEV
                     LVRQFDHFQEASWLSIYHGHGLRAAAYDPLIERIPLQTLRDVMRQRRESIRQTIVKAP
                     RHEDFIRQHCASEAFSVA"
     gene            615..2129
                     /locus_tag="VF402_02155"
     CDS             615..2129
                     /locus_tag="VF402_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7799123.1"
                     /translation="MLNSTPDPIRKIVIVGGGTAGWMTAAPLAQKLGAGCRVELVESA
                     EIGAVGVGEATLPTIRFYNRALGIDAADFIKKTQGTFKLGIEFRDWGHAGNRFFHGFG
                     DYGPRIDNRSPHHDWLRLRALGETAAFEDTSMASVMARHNVFAQPTGERPSPLNAFSY
                     AFHFDAGLYAAYLRDYATARGVTRTEGRIVDVALRPEDGFISHLILADGRRVEGDLFV
                     DCSGFRALLIEGALKSGFEDWSHWLPVNTALAVPSRRTAPLVPYTRCTARAAGWQWRI
                     PLQSRTGNGHVFCDAFTNEDEAARVLAAHLDAEALDAPRRIRFTTGRRKRSWVKNCVA
                     IGLSSGFLEPLESTSIQLIESGAGSLLELFPDLRFSPRLADEYNRRAAIWYENVRDFL
                     ILHYKLNDRPEPFWRYCRDMAIPDRLTHQIETFRETGHAIVYDKDGFTEPSWLALYFG
                     LGLTPCAYDPLADRIEAEAARTHFARIRESVARAVAAMPQAETYIERYLKAVPV"
BASE COUNT          406 a          832 c          761 g          419 t
ORIGIN      
        1 aagaactgcg tcgccatcgg cctgtcgtcg ggcttcctgg agccgctgga atcgacctcg
       61 atcaacttca tccagaacgc cgcggtgcgc ctgctggaac tgttcccgca aaagcagatc
      121 gacccgtgcc tgcgggacga atacaatcgg cgcaatcagg cctcctacga ctatgtgcgc
      181 gacttcatca tcgcccatta ctgcctgaac agccgcgacg gcgatctgtg gcgcgactgc
      241 gcgcgcatgg cgattcccga cgacctgcgc tggaagatcg agctgttcaa acgccgcggc
      301 gaagtgctgg tgcgtcagtt cgaccatttt caggaagcgt cctggctgtc catctatcac
      361 ggccacggcc tgcgcgcggc ggcctacgat ccgttgatcg agcgcatccc gctgcaaacc
      421 ctgcgcgacg tgatgcgcca gcgccgtgag tccatccgcc agacgatcgt caaggcgccc
      481 cgccacgaag atttcattcg tcagcattgc gcctcggaag ccttctcggt cgcctgagcg
      541 gcggcgaacc gggcttgatc atgcggcgcg gccggtttta tatgatgaaa atcatctaaa
      601 atggtccgcg ccctatgctt aattccacgc ctgaccccat tcgtaagatc gtcattgtcg
      661 gcggcgggac cgccgggtgg atgacggcgg cgccgctggc gcagaagctg ggcgcggggt
      721 gcagggtcga gctggtcgag agcgcagaga tcggcgcggt cggggtcggc gaggccacct
      781 tgccgaccat ccgcttctac aaccgcgcgc tggggatcga cgccgcagac tttatcaaaa
      841 agacgcaagg cacgttcaag ctcggcatcg agttccgcga ctggggccat gcgggcaacc
      901 gcttctttca cggcttcggc gattacgggc cgcgcatcga caaccgctcg ccgcatcacg
      961 actggctgcg gctgcgcgcg ctgggcgaga cggcggcgtt cgaggacacc tcgatggcca
     1021 gcgtcatggc gcgccacaac gtcttcgccc agccgaccgg cgagcggccc tcgccgctca
     1081 acgccttttc ctacgcgttc catttcgacg ccgggctgta tgcggcctac ctgcgcgact
     1141 atgcgacggc gcgcggcgtc acgcgcaccg agggccggat cgtcgacgtc gcgctgcggc
     1201 ccgaggacgg cttcatctcg cacctgattc tggcggacgg acggcgcgtc gagggcgacc
     1261 tgttcgtcga ctgttcgggc tttcgcgccc tgctgatcga gggcgcgctg aaaagcgggt
     1321 tcgaggactg gtcgcactgg ctgccggtca atacggcgct ggccgtcccg tcgcggcgca
     1381 cggcgccgct ggttccctat acgcgctgca cggcgcgcgc ggcgggctgg caatggcgca
     1441 tcccgctgca aagccgcacc ggcaacggcc atgtcttctg cgacgccttc acgaacgagg
     1501 acgaggccgc gcgcgtgctg gcggcgcatc tcgacgccga agcgctggac gcgccgcgcc
     1561 gcatccgctt cacgaccggc cgccgcaagc ggtcgtgggt gaagaactgc gtcgccatcg
     1621 gcctgtcgtc gggcttcctg gagccgctgg aatcgaccag catccagttg atcgaaagcg
     1681 gcgcgggcag ccttttggag ctgttccccg acctgcgctt cagcccgcgc ctggccgacg
     1741 aatacaaccg ccgcgcggcg atttggtacg agaacgtgcg cgacttcctc atcctgcatt
     1801 acaagctcaa cgaccgcccc gaaccgttct ggcgctattg ccgcgacatg gccatacccg
     1861 accggctcac gcatcagatc gagacgttcc gcgaaaccgg ccacgccatc gtctatgaca
     1921 aggacggctt caccgagccg tcgtggctgg cgctctattt cgggctgggc ctgacgccgt
     1981 gcgcctacga tccgctggcc gaccggatcg aggccgaggc cgcgcgcacg catttcgccc
     2041 gcatccgcga gtcggtcgcg cgcgccgtcg ccgccatgcc gcaggccgaa acctatatcg
     2101 aacgctacct gaaagcggtc ccggtttaaa tcagaagccg ttgatggcgt taacacatct
     2161 tttacgatac tctgtggaac aattgatcgc aaaagtggta tgaaacttga aagcccgacg
     2221 aatcggaacc ggcctgtgcc ggaaccgcac acgtccttcg gctttcgggt gttcgccatg
     2281 acagttcagc acgtttccct ctcgaacaag ccttccgccg ccagccccgc gccgcgcagc
     2341 ctcgacaccg acatcgccgt tctgggcgcc ggcgcggacg cggccctgac cgcgctggtc
     2401 ctggcgcgcg gcggccgc
//
LOCUS       DASVLL010000131         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_169318,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000131 DASVLL010000000
VERSION     DASVLL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169318"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VF402_02160"
     CDS             complement(<1..1712)
                     /locus_tag="VF402_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7799124.1"
                     /translation="MTLDRRRLLGLAAAAPAALSAVKAFAQAPDDSLVSGHLGQPAAI
                     PDAGWRLWLDPEAAWKDDVIYLPDDAPPLAQLPVNPPTGGWGALTASASREVTLPATV
                     EQFDWGRFGERPYTPDEYRYADDDPVPQNGAYAGVSWWWRDIDIPAAAGDAQVWLHLR
                     GARMRAEVFLNEKRVGYSIMSELPIDCDLTQAMRPGGPNRLAVRITNPGGRYDWKDSN
                     SLTWGHAKLYESHGFGGLDRGLYLSLHPKAGRIRDVWVLNTPQPRVVTAHLELALAEP
                     APVAALKKQVSAALYDDRGQPVAADVTLEDVTVDGATATARFRLGAPAAKLWDLDAPN
                     LYRVRLTWRDGKASDSAEARFGFRWYSPEGVGADAMLRLNGRRIKAYSAISWGYWGYN
                     GLWPTPELARREVTAAKTLGLNSLSFHRNVGKREVLNRQDEMGLLRSMEPGAGRQAIG
                     RDLRPGQTLSPADRFSRDFMLAKCRAMVRAFRSHPSLVHYTLQNEVSANLANPDVAAV
                     LRAMRDLDPSRTIILNDGFVARGAAQAMFLPYDDHLYRSDVEPAGGWWVNHQGAGDQW
                     YDKF"
     gene            complement(1715..2674)
                     /locus_tag="VF402_02165"
     CDS             complement(1715..2674)
                     /locus_tag="VF402_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEX7799125.1"
                     /translation="MKTLFLMTLCAAALIAAPALAQVQPTYFPAPPRLAPDQLPAVVD
                     AAKRAATKHIEPDKIILVGDSTTQVGSGWGGAFCDNHVTSFLACVNLARGGRGTFDYR
                     AEGSWDIALGEMKVPGYRHVYVLIQFGHNDQPGKPGRTTDLYTEYPANLARYVTEARA
                     AGAVPVLVTPLTRRSFRDGSLQADLDAWAKAARKVAADMNVPLVDLNKASTDAVQAMG
                     PVAAIALAETPPPQFVIDAAKTGTTVGAPKDGKPAPETANETLDTPQGHPLVVFDYTH
                     LGPEGAEYFAHMVTAQLAKVAPDLRRELVDKLYPAPYAMPAGY"
     gene            complement(2704..>3222)
                     /locus_tag="VF402_02170"
     CDS             complement(2704..>3222)
                     /locus_tag="VF402_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEX7799126.1"
                     /translation="YVNKGPQGRMSRDYFADCYIEGHVDFIFGNAKAYFRGCEIHGIP
                     HSEVMYTAQSKNAPDEDSGFVFDDCTFTAEPGAQGVSLGRAWRAYATVVLLNAKIETP
                     LIKGGWREWHPGQTQTLKTAYYAEYKSTGVGADPTGREPYSHQLTDAQAAQWKLPAFF
                     KGDRGWLPQDAR"
BASE COUNT          498 a         1080 c         1103 g          541 t
ORIGIN      
        1 tagaatttgt cgtaccactg atcgcccgcg ccctgatggt tgacccacca gccgcccgcc
       61 ggttcgacat cggagcggta gagatgatcg tcatagggca ggaacatcgc ctgcgccgcg
      121 ccgcgcgcga cgaagccgtc attgaggatg atggtgcggc tggggtcgag gtcgcgcatg
      181 gcgcgcaaca ccgccgcgac gtccgggttg gcgaggttgg ccgacacctc gttctgcaag
      241 gtgtaatgca ccaggctggg gtgcgaacgg aaggcgcgga ccatcgccct gcacttggca
      301 agcatgaaat cgcggctgaa gcggtcggcg ggcgacaggg tctgacccgg cctgaggtcg
      361 cggccgatgg cctgacggcc cgcgcccggc tccatgctgc gcagcaaacc catctcgtcc
      421 tgccggttca gcacctcgcg cttgccgaca ttgcggtgga agctgagcga attgaggccc
      481 agcgtcttcg ccgccgtcac ctcgcgccgc gccagctccg gcgtcggcca caggccgtta
      541 tagccccaat agccccatga gatcgccgag tacgccttga tgcgccgccc gttgagccgc
      601 agcatggcgt cggcgccgac gccttcgggg ctgtaccagc gaaagccgaa gcgcgcctcg
      661 gcgctgtccg acgccttgcc gtcgcgccat gtcaggcgca cgcggtagag gttgggcgcg
      721 tcgagatccc acagcttcgc cgccggggcg ccgaggcgga agcgcgccgt ggcggtcgcg
      781 ccgtccaccg tcacgtcctc cagcgtcacg tcggcggcga cgggctggcc ccggtcgtcg
      841 taaagcgcgg cgctcacctg ttttttcagc gccgcgaccg gggccggttc ggccagcgcc
      901 agttcgagat gcgcggtgac gacgcgcggc tgcggcgtgt tcagcaccca cacatcgcgg
      961 atgcggcccg ccttcgggtg caggctgaga taaagccccc ggtccagccc gccgaaaccg
     1021 tgcgattcgt agagcttcgc atggccccag gtcaggctgt tggaatcctt ccagtcatag
     1081 cgtccgcccg gattggtgat gcgcacggcc agccggttcg gcccgcccgg ccgcatggcc
     1141 tgcgtcaggt cgcaatcgat cggcagttcc gacatgatcg aatagccgac ccgcttttcg
     1201 ttgaggaaca cctcggcccg catccgcgcg ccgcgcagat gcagccacac ctgcgcgtcc
     1261 ccggcggcgg cggggatatc gatgtcgcgc caccaccacg acacgccggc ataggcgccg
     1321 ttttgcggca cgggatcgtc gtcggcgtag cggtattcgt ccggcgtata gggccgttcg
     1381 ccgaaccggc cccagtcgaa ctgctcgacc gtggcgggca aggtcacctc gcgcgaagcc
     1441 gacgccgtca gcgcccccca gccgccggtg ggcggattca ccggcagttg cgccagcggc
     1501 ggcgcgtcgt cgggcaggta gatgacgtcg tccttccacg ccgcttcggg gtccagccat
     1561 aaacgccagc ctgcgtccgg gatggcggcg ggctggccga gatggccgga gacgaggctg
     1621 tcgtccggcg cctgcgcgaa ggctttgacg gcggaaagcg ccgcgggcgc ggcggcggcg
     1681 aggcccagaa ggcggcggcg gtcgagcgtc atcctcaata ccccgccggc atggcgtagg
     1741 gcgccggata gagcttgtcg accagctcgc gccgcaggtc gggcgcgacc ttggccagtt
     1801 gcgccgtcac catatgggcg aaatactccg cgccttccgg gccgagatgg gtgtagtcga
     1861 acacgaccag gggatggccc tgcggcgtgt ccagcgtctc attagccgtt tcgggggcgg
     1921 gcttgccgtc cttgggcgcg ccgacggtcg tgccggtttt cgccgcgtcg atgacgaatt
     1981 gcggcggcgg cgtttcggcc agcgcgatcg ccgcgaccgg ccccatcgcc tgcaccgcgt
     2041 cggtcgacgc cttgttgaga tcgaccagcg ggacgttcat gtcggcggcc accttgcgcg
     2101 ccgccttcgc ccaggcgtcg agatcggcct gcaacgagcc gtccctgaac gaccgccgcg
     2161 tcagcggcgt caccagcacc gggaccgccc cggcggcgcg cgcctcggtc acatagcgcg
     2221 ccagattggc cggatactcg gtatagaggt cggtcgtgcg ccccggcttg cccggctggt
     2281 cgttatggcc gaactggatc agcacataga cgtggcggta gcccggaacc ttcatctcgc
     2341 ccagcgcgat gtcccatgag ccttcggcgc ggtaatcgaa ggtgccgcgc ccgccgcgcg
     2401 ccagattgac gcaggcgagg aacgaggtga cgtggttgtc gcagaaggcg ccgccccagc
     2461 cgctgccgac ctgggtggtg gaatcgccga ccagaatgat cttatccggt tcgatatgct
     2521 ttgtggccgc gcgtttggcg gcgtccacga cggcgggcaa ctgatcaggc gccagacgag
     2581 gcggcgcagg aaaataggtc ggctgaacct gcgccagcgc cggggccgct atcagggctg
     2641 cggcgcataa ggtcatcaaa aaaagcgttt tcatgtggcg tttccttttt tgttatgagg
     2701 ggcttagcgg gcgtcttgcg gcagccagcc cctatcgcct ttaaagaagg ctggcagctt
     2761 ccattgcgcg gcctgcgcat cggtgagttg atggctgtac ggttcgcggc ccgtcggatc
     2821 agcgccgacg ccggtcgatt tatattcggc gtaataggcg gtcttcagcg tctgggtctg
     2881 acccggatgc cattcgcgcc aaccgccctt gatcagcggc gtctcgatct tcgcgttcag
     2941 caacacgacg gtggcatagg cgcgccaggc gcggccgaga ctgacgccct gcgcgcccgg
     3001 ctccgccgtg aaggtgcaat catcgaagac gaagccgctg tcctcgtccg gcgcgttctt
     3061 gctctgcgcc gtgtacatga cctcggaatg cgggatgccg tgaatctcac agccccggaa
     3121 ataagccttg gcgttgccga aaatgaaatc gacatggccc tcgatatagc agtccgcgaa
     3181 atagtcgcgg ctcatgcggc cctgcggccc cttgttgacg ta
//
LOCUS       DASVLL010000132         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_437759,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000132 DASVLL010000000
VERSION     DASVLL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437759"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..>1952
                     /locus_tag="VF402_02175"
     CDS             31..>1952
                     /locus_tag="VF402_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7799127.1"
                     /translation="MARETSDFIDDLRLSILNDRLERRAKESDSPFVAAEAGRDTVGE
                     TAEVTQLIVTPKPGQDKAALAAAYPMTRQFATYGVTPDELSRALADQEARFQAAVKGA
                     NTRESRHLADELADSMGNGDVFTSPQQDYDYFEALKPGLTVARLNDGVKALFGGDGPF
                     LWHEGENLNGLDRQALLDTYNAVQSAPVAKPDIQATKPWPYTDFGAPAKVVKRQEVKD
                     LGLVQLTYANGVRATIKTTDFKKDEIGVTVRFAGGLSVLSPAARPPVFQATVSSLMEG
                     GLGKLTAAEIKDTLSGKIYSVQYGLGDDAATLSGGTTKADFATEMQALMAFTTDAAMR
                     DDAFERLKGFIPDYYQQLQSTPGGVFQMQANGVLHDGDARFVMPSQQDFLATKNADVK
                     ALVEGELKTAPVEITIVGDVDEAEAEAEIAKTFAALPARPAAPKTPAADLKVDFPAAN
                     LRRVFEHHGRADQDLSFIAWPGADFTSDPARASGLTVLSEVLNLRLTDVVREQKGIAY
                     SPYASNVNSQTFPGYGYLSATAEVKPENDQAFYDAVAGIVADLKARPIDADELLRAQK
                     PLLDQMDTQLKTNSYWQSVLPGTMSDPRKLDYIRSRRARYQAVTAADIQKLAQEYLNM
                     DKALRIQIKPAADAAPA"
BASE COUNT          374 a          703 c          612 g          263 t
ORIGIN      
        1 gtccgacgtt tccctgacca ttatcaggac gtggccaggg aaacgtcgga cttcatcgac
       61 gacctgcggc tgagcatcct caacgaccgg ctggagcggc gcgccaaaga gagcgactcg
      121 cccttcgtcg ccgccgaggc cggacgcgac acggtcggcg agaccgccga ggtcacgcag
      181 ctcatcgtca cgcccaagcc ggggcaggac aaggccgccc tcgccgccgc ctaccccatg
      241 acgcgccagt tcgcgaccta tggcgtgacg ccggacgagc tgagccgcgc gctggccgat
      301 caggaggccc gcttccaggc cgccgtcaag ggcgccaaca cgcgcgaaag ccgccatctg
      361 gccgacgaac tggccgacag catgggcaac ggcgacgtct tcacctcgcc gcagcaggat
      421 tacgattatt tcgaggcgct gaagcccggc ctgacggtgg cgcgcctcaa tgacggcgtg
      481 aaggctttgt tcggcggcga cggtccgttc ctgtggcacg agggcgaaaa cctgaacggc
      541 ctcgacaggc aggccctgct ggacacctat aacgccgtgc agtcggcgcc ggtcgccaaa
      601 cccgacatcc aggcgacgaa gccgtggccc tacaccgact tcggcgctcc ggccaaggtg
      661 gtcaagcgcc aggaggtcaa ggatctgggc ctcgtgcaac tgacctacgc caacggggtc
      721 cgggccacga tcaagaccac cgatttcaag aaggacgaga tcggcgtcac ggtgcgcttc
      781 gcgggcggcc tctccgtcct gtcgcccgcc gccaggccgc cggtgtttca ggccaccgtc
      841 tccagcctga tggagggcgg cctcggcaag ctgaccgccg ccgagatcaa ggatacgctc
      901 agcggcaaga tctacagcgt gcaatacggc ctcggcgacg atgcggccac cctgagcggc
      961 ggcacgacca aggccgattt cgccaccgag atgcaggcgc tgatggcctt caccaccgac
     1021 gcggcgatgc gcgacgacgc cttcgagcgg ctcaagggct tcatccccga ctattaccag
     1081 caactgcaat ccacgcccgg cggcgtgttc cagatgcagg cgaacggcgt gctgcacgac
     1141 ggcgacgccc gcttcgtcat gcccagccag caggatttcc tcgccacgaa aaacgccgac
     1201 gtcaaggcgc tggtcgaggg cgagctgaag accgcgccgg tcgagatcac catcgtcggc
     1261 gacgtcgacg aagccgaggc cgaggcggaa atcgccaaga ccttcgccgc cctgcccgcg
     1321 cgtcccgccg cgccgaaaac gccggcggcc gacctcaagg tcgacttccc cgccgccaat
     1381 ctgcgccggg tgttcgagca tcacggccgc gccgatcagg atctgagctt catcgcctgg
     1441 ccgggggcgg acttcacctc cgatccggcg cgcgcctcgg gcctgaccgt gctgtccgaa
     1501 gtgctgaacc tgcgcctgac cgacgtggtg cgcgagcaga agggcatcgc ctacagcccc
     1561 tacgccagca acgtcaattc gcagaccttc cccggctacg gctatctgag cgccaccgcc
     1621 gaggtgaagc ccgaaaacga tcaggccttc tatgacgcgg tggccggaat cgtcgccgac
     1681 ctcaaggcca ggccgatcga cgccgacgaa ctcctgcgcg cgcagaagcc gttgctggac
     1741 cagatggaca cccagctcaa gaccaacagc tactggcaga gcgtgctgcc cggcacgatg
     1801 agcgatccga ggaagctcga ctatatccgc agccgccgcg cccgctatca ggcggtcacc
     1861 gccgccgaca tccagaagct ggcgcaagag tacctgaaca tggacaaggc cctgcgcatc
     1921 cagatcaaac ccgccgccga cgccgcgcct gc
//
LOCUS       DASVLL010000133         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_61040, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000133 DASVLL010000000
VERSION     DASVLL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61040"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VF402_02180"
     CDS             complement(<1..1136)
                     /locus_tag="VF402_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799128.1"
                     /translation="MTDRSGARGAMSGYPAGRYRWAICALLFAATAINYVDRQMIGVL
                     KPTLQKDLGWSETAYADIVFWFQAAYAIGYLSFGRIIDRLGARIGYAVTFTIWTLSHM
                     AHGLVGNVTQFALARFSLGLGESGSFPASLKAVSEWFPQKERALAVGLFNAGANVGAI
                     LTPLIVPAITLAWGWRMAFFITGGVSLIWLAAWLGLYRRPERHKRVTPAELAYIRQDA
                     PAAEGEKASFGRILLTRETWAYALVKFLTDPVWWLYLFWLPDFLHKTYGLDLKSFGPP
                     LVAVYVLSDIGSVLGGWGSSRLVAKGVSVNRARKTAMFICALLVLPIVFAQFVSHLWL
                     AVLIVGVAAAAHQAYSANLMTLPSDLFPRAAVGTVVGIGGTAGAV"
     gene            complement(1177..>1878)
                     /gene="kduI"
                     /locus_tag="VF402_02185"
     CDS             complement(1177..>1878)
                     /gene="kduI"
                     /locus_tag="VF402_02185"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEX7799129.1"
                     /translation="QERMVVGSASPLMQPLALPDDGGRPFLERRELGAINIGAGAGQV
                     RVDGVAHEVEPLGALYAGMGARDVVFESPDPARPARFYLASTPAHAASPTRCLPRSLI
                     APQRRGDADSANQRLIYQYVVPGACGSAQLLMGLTLMNAGNVWNTMPPHTHERRSEVY
                     FYFDLPPDKPVFHFMGRRDETRHIVVRNEEAVICPPWSLHMGVGAAAYAFIWAMGGEN
                     LDYDDMQGVDLDGLS"
BASE COUNT          338 a          637 c          630 g          273 t
ORIGIN      
        1 accgcgcccg ccgttccgcc tatgccgacg accgtgccga ccgccgcgcg cggaaacagg
       61 tcggagggca aggtcatcag gttggcggaa taggcctgat gcgcggcggc ggccacgccg
      121 acgatgagga ccgccagcca cagatggctg acgaactgcg cgaacacgat gggcaggacg
      181 aggagcgcgc agatgaacat ggccgtcttg cgcgcgcggt tgacgctgac gcccttggcg
      241 accagacgcg acgagcccca gccgccaagc acgctgccga tatcggaaag aacatagacg
      301 gcgaccagcg gcggcccaaa gctcttcaag tccagcccgt aggtcttatg cagaaaatcc
      361 ggcagccaga acaggtacag ccaccacacg ggatcggtca ggaacttgac cagcgcatag
      421 gcccaggttt cccgcgtcag gaggatcctg ccgaaggacg ccttttcgcc ttcggccgcc
      481 ggcgcgtcct gccggatata ggccaactcg gcgggcgtga cgcgcttgtg acgctccggc
      541 cgccggtaca ggcccagcca tgcggccagc cagatcaggc tgacgccgcc cgttatgaag
      601 aaggccatgc gccagcccca cgccagcgtg atcgccggga cgatcagagg cgtcaggatg
      661 gccccgacat tggccccggc gttgaacagg ccgaccgcca gcgcgcgctc cttctgcggg
      721 aaccactccg acacggcttt caggctggcc gggaagctgc ccgattcgcc gaggcccagc
      781 gaaaagcgcg ccagggcgaa ctgggtcaca ttgccgacca gaccgtgcgc catgtgcgac
      841 agcgtccaga tggtgaaggt caccgcatag ccgatccgcg cgccgagccg gtcgatgatg
      901 cggccgaaac tgagatagcc gatggcgtag gcggcctgaa accagaacac gatgtccgca
      961 taggcggttt cgctccagcc cagatccttt tgcagggtcg gcttcagcac gccgatcatc
     1021 tggcggtcga catagttgat ggcggtggcc gcgaacagga gcgcgcagat ggcccacctg
     1081 tagcgtcccg ccggatatcc actcatcgcg ccgcgcgcgc cggacctgtc cgtcatgttg
     1141 cgtttccctg ttgtcttcgt ctcttggcgg gcgcggtcag ctcaggccgt cgaggtccac
     1201 gccctgcatg tcgtcatagt cgaggttctc gccgcccatc gcccagataa aggcgtaggc
     1261 ggcggcgccg acgcccatgt gcagcgacca cggcgggcag atgaccgcct cctcgttgcg
     1321 cacgacgata tggcgcgtct cgtcgcgccg ccccatgaag tgaaagacgg gtttgtccgg
     1381 cggcagatcg aaatagaaat agacttccga gcgccgttca tgggtgtgcg gcggcatggt
     1441 gttccagaca ttgcccgcgt tcatcagggt cagccccatc agcaactgcg ccgaaccgca
     1501 ggcgccggga acgacgtact gatagatcag gcgctgattg gcgctgtccg cgtcgccgcg
     1561 ccgctgcggc gcgatgagcg aacgcggcag gcagcgcgtc gggctggccg cgtgcgcggg
     1621 cgtgctggcc agataaaagc gcgcgggacg cgcggggtcc gggctttcga acaccacgtc
     1681 gcgcgcgccc atgccggcat agagggcgcc cagcggctcc acctcgtgcg ccacgccgtc
     1741 aacgcggacc tgtccggcgc ccgcgccgat attgatcgcg cccagctccc ggcgctccag
     1801 aaagggcctg ccgccgtcgt ccggcagggc caggggttgc atcaggggcg aggccgagcc
     1861 gacgaccatg cgctcctg
//
LOCUS       DASVLL010000134         4139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_169651,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000134 DASVLL010000000
VERSION     DASVLL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169651"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF402_02190"
     CDS             complement(<1..516)
                     /locus_tag="VF402_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX7799130.1"
                     /translation="MPSLLAAFKALFRPWAAKNGAAKDRAAKDTGARLYAQCVAQARL
                     PVFYLDYAVPDEIGARFELLTFHVGLVIHALRVVKRDDARHEQAQETAQALFDSFLLA
                     LDSTLREQGTGDLSVPKKMKKLGQVVYTRMQRWETLWAAGAGPQADYAARTLYAGTGL
                     DDMGETGEAAAD"
     gene            693..1178
                     /gene="bamE"
                     /locus_tag="VF402_02195"
     CDS             693..1178
                     /gene="bamE"
                     /locus_tag="VF402_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX7799131.1"
                     /translation="MKTSLSLTALAALAALSLSACAPTMTHHGYLAIDARPSTDIKVG
                     ESQATILDKFGAPSQTSIYDPQEWYYIDQVSMKMTYKQPHVTARSVTIVDFDKATQTV
                     SDVRTLSLADGRDLTPDPHKTPTRGRQLTALEQVLGTVGHQQLTNDQDSNPGNNNHRR
                     D"
     gene            complement(1252..3405)
                     /locus_tag="VF402_02200"
     CDS             complement(1252..3405)
                     /locus_tag="VF402_02200"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX7799132.1"
                     /translation="MNIALMLAIAAGILALIYGVAQTLGLMKADAGSERMREIAAAIQ
                     EGASAYLKRQYTTIAIVGVVIFVLAWVFLGQWSAVGFAIGAILSGAAGFAGMLISVRA
                     NVRTAQAASQSLQKGLKLAFSAGAVTGLFVAGGALLGVSGYYAILTLVLKFEPASRDV
                     VNALVSLGFGASLISIFARLGGGIFTKGADVGGDMVGKVEAGIPEDDPRNPATIADNV
                     GDNVGDCAGMAADLFETYAVTTVATMVLATIFFGGSDLLGAMMMLPLAIGGVCIVTSI
                     IGTFFVSLGKSQDIMGALYKGLIATGILSVLAVAGVIWKFVGFDTAIAYTLGGATYSF
                     TGMSLFWCGMVGLAVTAAFIVVTEYYTGTGKRPVVSIAQASVTGHGTNVIQGLAVSME
                     STAIPALIIVAGIIGCYLLAGLFGIAIATSTLLALAGIIVALDAFGPVTDNAGGIAEM
                     AGLPADVRHTTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTSDLKYFAANSTGG
                     IFEGLGNLTFSLSSPWVVVGLLIGGLLPYLFGGMGMTAVGRAAQSVVEEVRRQFREDS
                     GIMAGTSKPNYGRAVDMLTKAAIREMIVPSLLPVLSPIVLFVIVYYIGGKTPAFESLG
                     AMLLGVIVTGLFVAISMTSGGGAWDNAKKSFEDGFVDKDGVKHLKGSEAHKASVTGDT
                     VGDPYKDTAGPAVNPMIKITNIVALLMLAVLAHAG"
     gene            complement(3682..4089)
                     /locus_tag="VF402_02205"
     CDS             complement(3682..4089)
                     /locus_tag="VF402_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799133.1"
                     /translation="MSLSRRRFLLILPFCAAASARAETKNVAAPSVQLATVGIPVMRD
                     NRLVNYLFLSIRVNLTGKANEAKLRDMEPYFRDAIVRAASKTSFAQADNEDVLDAPRF
                     KAAMIPEFEAFAGRGAIKSLDILAQNPKIHHHF"
BASE COUNT          810 a         1397 c         1250 g          682 t
ORIGIN      
        1 atccgccgcg gcctcgcccg tctctcccat atcgtcaaga ccggtcccgg cgtaaagggt
       61 gcgcgcggcg taatccgcct gcggcccggc gccggcggcc cacagcgttt cccagcgctg
      121 catccgcgta tagacgacct gacccagctt tttcatcttt ttcggcacgc tgagatcgcc
      181 cgtcccctgt tcgcgcagcg tcgaatcgag cgccagcagg aacgaatcga acagggcctg
      241 cgcggtttcc tgcgcctgct cgtggcgcgc atcgtcccgc ttcacgaccc gcagcgcatg
      301 gatgacaagc ccgacatgaa aggtaagcaa ctcgaagcgc gcgccgatct cgtccggcac
      361 ggcgtaatcg aggtaaaaga ccggcagccg cgcctgcgcc acgcattgcg catacaggcg
      421 cgcgcccgta tccttggccg ccctatcctt ggcagcccca ttcttggcag cccaagggcg
      481 gaacagggcc ttgaacgcag cgagcagcga cggcatgacg gtgggctccc ctgatgacga
      541 aaccggcgcc ggttgcggcg gcgcggcctt ctttcagcct tgagcttgcc gtaagcttaa
      601 ttttcttata ggtcaaatgt cttagggttt cgggcgcgct tcctgtcttg cgcgcttccg
      661 gccatgttaa caggatttcc atgcggttcg gtttgaaaac ttcgctttcc ctgacggctc
      721 tggcggccct tgcggcgctg agcctgagcg cctgcgcccc gaccatgacg catcatggct
      781 atctggccat cgacgcccgg ccgtcgaccg acatcaaggt cggcgagagc caggccacca
      841 tactcgacaa gttcggcgcc ccgtcgcaaa cctcgatcta cgatccgcag gaatggtact
      901 atatcgacca ggtgtcgatg aagatgacct acaagcagcc gcacgtcacg gcgcgctcgg
      961 tcaccatcgt cgatttcgac aaggcgacgc aaaccgtgtc ggacgtccgc accctgtcgc
     1021 tggccgacgg gcgcgacctg acgcccgacc cgcacaagac cccgacgcgc gggcgtcagt
     1081 tgaccgcgct ggagcaggtg ctcggcacgg tcggccacca gcagctgacc aacgatcagg
     1141 acagcaaccc cggcaacaac aaccaccgcc gcgactgagc cttttcagaa accagagacg
     1201 aaaaaagccc cggagcgatc cggggctttt ctctgtgcat cgaggctcag atcatcccgc
     1261 gtgcgccagc acggccagca tcagcagcgc gacgatgttg gtgatcttga tcatcggatt
     1321 gaccgccggt cccgccgtgt ccttgtacgg atcgccgacc gtgtcgccgg tcaccgacgc
     1381 cttgtgcgcc tccgagcctt tcaggtgctt cacgccgtcc ttgtcgacga agccgtcctc
     1441 gaacgacttt ttcgcattgt cccacgcgcc gccgcccgag gtcatcgaga tggcgacgaa
     1501 caggccggtg acgatcacgc ccagcagcat ggcgcccagc gattcgaacg ccggggtctt
     1561 gccgccgata taatagacga tgacgaacag cacgatcggc gacagcaccg gcagcagcga
     1621 cggcacgatc atttcgcgga tcgccgcctt ggtgagcata tcgaccgcgc ggccgtaatt
     1681 cggcttcgac gtcccggcca tgatgccgga atcctcgcgg aactgacggc gcacctcctc
     1741 gaccacggac tgcgcggcgc ggcccacggc ggtcatgccc atgccgccga acaggtaggg
     1801 caaaagcccg ccgatcagca gcccgaccac gacccacggc gaggacagcg agaaggtcag
     1861 gttgcccagc ccctcgaaaa tcccgccggt cgaattggcg gcgaaatatt tgaggtcgga
     1921 tgtataggct gcgaacagca ccagcgcgcc gaggcccgcc gagccgatgg cgtagccctt
     1981 ggtcaccgcc ttggtggtgt tgccgaccgc atcgagcgcg tcggtggtgt ggcgcacgtc
     2041 ggcgggcagc cccgccattt cggcgatgcc cccggcgttg tcggtcaccg gcccgaaggc
     2101 gtcgagcgcg acgatgatcc cggccagggc cagcagcgtg ctggtggcga tggcgatgcc
     2161 aaacagcccg gccagcaggt aacagccgat aatgcccgcc acgatgatca gcgccggaat
     2221 cgcggtcgat tccatcgaca cggccagccc ctgaatgacg ttggtgccgt ggccggtcac
     2281 cgaggcctgc gcgatcgaaa cgaccgggcg cttgcccgtg ccggtgtaat attcggtcac
     2341 gacgatgaag gccgccgtca ccgccagccc gaccatgccg caccagaaca gcgacatgcc
     2401 ggtgaagctg taggtcgcgc cgcccagcgt gtaggcgatg gccgtgtcga agccgacgaa
     2461 tttccagatc accccggcca ccgccagcac ggacaggatg cccgtggcga tcagcccctt
     2521 gtacagcgcg cccatgatgt cctgcgactt gccgaggctg acgaagaagg tgccgatgat
     2581 cgaggtcacg atgcacacgc cgccgatggc cagaggcagc atcatcatgg cgcccagaag
     2641 atcggacccg ccgaagaaga tggtggccag caccatggtc gccacggtgg tgaccgcata
     2701 ggtctcgaac aggtccgccg ccatgcccgc gcagtcgccg acattgtcgc cgacattgtc
     2761 cgcgatggtg gccgggttgc gcggatcgtc ctccgggatg cccgcctcga ccttgccgac
     2821 catgtcgccg ccgacgtcgg cgcccttggt gaagatgccg ccgcccagac gcgcgaagat
     2881 cgagatcagc gacgcgccga agcccagcga caccaacgcg ttgaccacgt cgcggctggc
     2941 cggttcgaac ttgaggacaa gggtcaggat cgcataataa cccgacacgc ccagcaaagc
     3001 gccgcccgcc acgaacaggc cggtgaccgc ccccgcgctg aaggccagct tcagcccttt
     3061 ttgcaggctc tgcgacgccg cctgcgccgt gcgcacattg gcgcgcaccg agatcagcat
     3121 cccggcgaag cccgccgcgc ccgacaggat cgcgccgatg gcgaagccga cggccgacca
     3181 ctggcccaga aacacccacg ccagcacgaa gatgaccacg ccgacgatgg cgatggtggt
     3241 gtattgacgt ttcagatagg cgctcgcccc ttcctgaatg gcggcggcga tctcacgcat
     3301 acgctcgctt cccgcatcgg ccttcatcag gccgagagtc tgcgcgaccc cgtaaatcag
     3361 ggccagtatg ccggccgcga tggccagcat caaagcaata ttcataatgg tacccctgtc
     3421 ctagtctctc gttatgacgg gcgcgacgcg ccgccggtta tggcgaacca aaaaataatc
     3481 gaaattcaat cgcttacttt ttgattgtca gggagagtgc caaaagtttc agccgaacac
     3541 aagccgggat aacaatgcta tcgcaccgca gcgcaagttt tcgcacacgc gaattacgca
     3601 gcgcggcata tcgaagactt gaaaagcggc cttcatgcgc aaattcagag gcttgaaatg
     3661 cgcgcaatgc agcattcggt gtcagaaatg atgatgaatt ttcggattct gcgccagaat
     3721 atccagcgat ttgatcgcgc cccggccggc gaacgcttcg aattcgggga tcatcgccgc
     3781 cttgaagcgc ggcgcgtcca gcacgtcctc gttatccgcc tgcgcgaacg aggtcttgct
     3841 ggcggcgcgc acgatggcgt cgcggaagta gggctccatg tcgcgcagct tcgcctcgtt
     3901 cgccttgccg gtcaggttca cgcggatgct caggaacagg tagttgacca gccggttgtc
     3961 gcgcatgacc ggaatcccga ccgtcgccag ttgcaccgac ggcgcggcga cgttcttcgt
     4021 ctcggcgcgc gcggaagccg ccgcgcaaaa cggcaaaatc agcaagaaac gacggcgcga
     4081 caggctcatg tccgccacct tggccgcaaa cgctttaaga tgcgcttaaa ataaaagcc
//
LOCUS       DASVLL010000135         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_277099,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000135 DASVLL010000000
VERSION     DASVLL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277099"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VF402_02210"
     CDS             <1..123
                     /locus_tag="VF402_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX7799134.1"
                     /translation="DDADTQAKWYQALKKEWLASRAEKRPFVNPVKPAVLKWTS"
     gene            287..886
                     /locus_tag="VF402_02215"
     CDS             287..886
                     /locus_tag="VF402_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7799135.1"
                     /translation="MKLFVNSGSPFARKARIVVRERGWAPGVEEVFTVPVESPDDLVG
                     ANPVAQIPALIGDDGVCWQDSTLICAWLDAKGEGAPLLPPADSAAYWAVRRLEAAAVA
                     LIEMNVRMVLEDRRPENERSPFWLRRWEDNLVRGFTRVETMCPAADVFDMGTLTLGVA
                     GTYTTFRYPHIGWRDIAPKAAALTDALERRQSFIETFPK"
     gene            928..2001
                     /gene="prfA"
                     /locus_tag="VF402_02220"
     CDS             928..2001
                     /gene="prfA"
                     /locus_tag="VF402_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7799136.1"
                     /translation="MHLPQAKLDQVMDRFRMIEARMESATDGAEIVRLSKEHAEIKPV
                     CEAVEKLVRARAQVPELEEMAGMNDPELSPLAEEELSGLKASLPELERGVALLLAPKD
                     KDENASAILEIRAGTGGDEAALFAGDLFRMYSRYASTQGWRVEVDSVSEGDAGGYKEI
                     IASVTGEGVFGKLKFESGVHRVQRVPATEAGGRIHTSAATVAVLPEAEDVEIEILDKD
                     IRIDTYRASGSGGQHVNKTDSAVRITHLPTGIVATSSEKSQHQNRQIAMRNLKARLYD
                     MQRTALDEARSSARKSQVGSGDRSERIRTYNYPQGRVSDHRINLTLYNLPQFMEGDMD
                     QMIGALIAEDQAERLAMLEEEFG"
     gene            2033..2413
                     /locus_tag="VF402_02225"
     CDS             2033..2413
                     /locus_tag="VF402_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799137.1"
                     /translation="MEKSRLRSVFAIAAGLAAMVAAGVWAAAGFGLRDGILALCLTVV
                     FMGALLPLTPPHRRRLRLCLKAVLVALIATICGVALYGLDVANLLAAAAKSWGTTLMV
                     LYLPVMALIGFGWRAWTRWPKPKA"
     gene            2392..3219
                     /locus_tag="VF402_02230"
     CDS             2392..3219
                     /locus_tag="VF402_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7799138.1"
                     /translation="MAEAKGVNVSGIVSSLWRHPVKGFTPENVPEALLSPGDFFPFDR
                     LFALEVGRSGFDPGDPKTIPKMRFAVLARFPAVARLRTRYDEYDDALELIDEDGKVWR
                     FGMSDPAGRHALARHVETVLARHEDYDPVALPLRLLTAPDFDHARRHFRFTDSGKGFV
                     SLLNLNSLRDLGQRLGREIDPLRLRGNIWVEGWAAFEDHAFVGKSIRVGEAGPEFEVL
                     KPIERCAATHVNPDTAERDIDLCGALFDNYGHRHCGVYARVAKGGVIRPGDRLHVLG"
     gene            3258..3974
                     /gene="gpmA"
                     /locus_tag="VF402_02235"
     CDS             3258..3974
                     /gene="gpmA"
                     /locus_tag="VF402_02235"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX7799139.1"
                     /translation="MPTLVLLRHGQSQWNLENRFTGWVDVDLTAEGEAQARRGGELIK
                     AEGVAIDQAFTSVLTRAIRTCNLALVAAGQSYVPVIKDWRLNERHYGGLTGLDKAETA
                     AKHGDEQVKIWRRSYDVPPPPLGADSPYDFKGDRRYAGANLPDTESLKTTLDRVLPMW
                     EGEIAPELKAGKTVLVAAHGNSIRAILKSLFHLSEQAILDVEVPTGNPLVIQLDNDLK
                     PVSAKYLDAARANPVPVYGA"
     gene            4129..>4750
                     /locus_tag="VF402_02240"
     CDS             4129..>4750
                     /locus_tag="VF402_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX7799140.1"
                     /translation="MRQGDNVAEARLWLALRGRQSGGLKFRRQFPIGPYIADFACPER
                     RLVVELDGGQHIDNPRDVVRDRYLNENGWSVARFSAGDVLSDKSIVLDTLLAICEARI
                     YQPVESYEFRFYPARLEMMEATPHPDPLPLQGEREQDVMYMRRAIELARGQLGRTWPN
                     PPVGCVLVKDGSVIAEAATGDGGRPHAEEAALAVAGAAARGATAYVT"
BASE COUNT          832 a         1511 c         1604 g          803 t
ORIGIN      
        1 gacgacgccg acacgcaggc gaaatggtat caggcgctga aaaaggaatg gctggccagc
       61 cgcgccgaaa agcggccctt cgtcaacccg gtcaagcccg ccgtgctgaa atggacgagc
      121 taagcgtttc ccccaaaaag cagctccacc ttttggataa ggaagagcga caaaacaaaa
      181 acttagagcg ctttccgaaa agtgtgacgc acttttcgga ttaaaaagcg cgtaaaagca
      241 tgaagttaga gcgcccgcca caatccccct gtccacgagg cttcgtatga aactgtttgt
      301 caattccggc tcgcccttcg cgcgcaaggc ccgcatcgtc gtgcgcgaac gcggctgggc
      361 gcccggcgtg gaggaggtct tcaccgtgcc ggtcgaaagc cccgacgatc tggtcggcgc
      421 caatccggtg gcgcagattc cggcgctgat cggcgatgac ggcgtgtgct ggcaggattc
      481 gacgctgatc tgcgcctggc tggacgcgaa gggcgagggc gcgccgcttc tgccgccggc
      541 ggacagcgcg gcgtactggg ccgtgcgccg tctcgaagcc gccgccgtcg cgctgatcga
      601 gatgaatgtg cgcatggtgc tggaggaccg ccgcccggaa aacgagcgct cgcccttctg
      661 gctgcggcgc tgggaggaca acctcgtgcg cggctttacg cgcgtcgaga cgatgtgtcc
      721 ggcggcggat gtgttcgata tggggacgct gaccttaggc gtggccggaa cctacaccac
      781 cttccgttac ccgcatatcg gctggcgcga catcgcgccg aaggcggcgg cgctgaccga
      841 cgcgctggag cggcggcaaa gctttattga aaccttccct aaataagtct aggcaggcgg
      901 cgttgcagct ttgagagaca cgccttgata cacctgcccc aagccaagct cgatcaggtc
      961 atggaccgct tccgcatgat cgaggcgcgc atggaatccg ccacggacgg cgccgagatc
     1021 gtgcgcctgt ccaaggaaca cgccgagatc aagccggtgt gcgaggcggt ggaaaagctg
     1081 gtccgcgcgc gcgcgcaggt tcctgagctt gaggagatgg ccgggatgaa cgatcccgaa
     1141 ctgtcgccgc tggccgagga ggagctgtcc gggctgaagg cgtccctgcc ggagctggag
     1201 cgcggcgtgg cgcttctgct ggcgcccaag gacaaggacg agaacgcctc ggccatcctt
     1261 gaaatccgcg cgggcaccgg cggcgacgag gcggcgctgt tcgcgggcga cctgttccgc
     1321 atgtattcgc gctacgcctc gacgcagggc tggcgcgtcg aggtcgacag cgtgtccgag
     1381 ggcgatgcgg gcggttacaa ggaaatcatc gcctcggtga cgggcgaggg cgtgttcggc
     1441 aagctgaaat tcgagtccgg cgtgcatcgc gtgcagcgcg tcccggccac cgaggcgggc
     1501 ggccgcatcc acacctcggc ggcgaccgtg gccgtgctgc ccgaagccga ggacgtcgag
     1561 atcgagattc tcgacaagga tatccgcatc gacacctacc gcgcctccgg ctccggcggc
     1621 cagcacgtca acaagaccga ttcggcggtg cgcatcaccc acctgccgac cggcatcgtc
     1681 gcgaccagca gcgaaaagtc gcagcaccag aaccgccaga tcgccatgcg caacctcaag
     1741 gcgcgcctct acgacatgca gcgcaccgcg ctcgacgagg cccgctccag cgcgcgcaag
     1801 agccaggtcg gctccggcga ccgctccgaa cgcatccgca cctacaacta tccgcagggc
     1861 cgcgtctcgg accaccgcat caacctgacc ctctacaacc tgccgcaatt catggaaggc
     1921 gacatggacc agatgatcgg cgccctgatc gccgaggatc aggccgaacg gctggccatg
     1981 cttgaggaag aattcggtta ggcgtacatt gccgtaaatg agggagggac gcatggaaaa
     2041 atcgaggtta cgcagcgtgt tcgccatagc cgccggtctc gccgccatgg tcgcggcggg
     2101 cgtatgggcc gccgccggct ttggcctgcg cgacggcata ctggcgcttt gtctgacggt
     2161 tgttttcatg ggcgctcttt tgccgttgac gccgccgcac cggcgccgcc tgcgcctgtg
     2221 tctgaaggcc gtgctggtcg cgctgatcgc gacgatatgc ggcgtggcgc tctatgggct
     2281 ggacgtcgcg aacctcttgg cggcggccgc gaagagctgg gggacgaccc tgatggttct
     2341 gtatcttccg gtcatggcgc tgatcgggtt cggctggcgg gcctggacaa gatggccgaa
     2401 gccaaaggcg tgaacgtttc cgggatcgtc tcctcgctgt ggcggcatcc ggtcaagggg
     2461 ttcacgccgg aaaacgtacc ggaggcgctg ttgtcgcccg gcgatttctt tccgttcgac
     2521 cgtttgttcg cactggaggt cgggcgcagc ggtttcgatc ccggcgatcc gaagaccatc
     2581 cccaagatgc gcttcgccgt gctggcgcgt tttcccgccg tggcgcggct gcgcacccgc
     2641 tatgacgaat acgacgacgc gctggagctg atcgacgagg acggcaaggt ctggcgtttc
     2701 ggcatgagcg atccggcggg ccgtcacgcg ctggcccggc acgtcgagac ggtgctggcg
     2761 cggcatgagg attacgatcc ggtcgccctg ccgctcaggc tgctgacggc gcccgatttc
     2821 gatcatgcgc ggcggcattt ccgcttcacc gattccggca agggcttcgt gtctttgctc
     2881 aacctcaact ccctgcgcga tctgggccag cggctcgggc gcgagatcga tccgctgcgc
     2941 ctgcgcggca atatctgggt cgagggctgg gcggcgttcg aggatcacgc tttcgtcggc
     3001 aaatcgatcc gcgtcggcga ggcggggccg gaattcgagg tgctgaaacc gatcgagcgc
     3061 tgcgcggcca cccatgtgaa cccggacacc gccgagcgcg acatcgatct ctgcggcgcc
     3121 ctgttcgaca attacggcca tcgccattgc ggcgtctatg cgcgcgtcgc gaagggcggc
     3181 gtcatccgcc ccggcgaccg cttgcatgtg ctgggctgaa accttatagc caaggcggat
     3241 atattcagga gccgcccatg cccaccctcg ttcttctccg ccacggccag agccagtgga
     3301 acctcgaaaa ccgcttcacc ggctgggtgg acgtcgatct gacggcggaa ggcgaggcgc
     3361 aggcccggcg tggcggcgag ctgatcaagg ccgaaggcgt cgcgatcgat caggcgttca
     3421 cctccgtgct gacgcgcgcc atccgcacct gcaatctggc cctggtcgcg gcgggtcaga
     3481 gctatgtgcc ggtgatcaag gactggcgtt tgaacgaacg tcattacggc ggcctgaccg
     3541 ggctggacaa ggccgaaacc gcggcgaaac acggcgacga gcaggtgaag atctggcgcc
     3601 gctcctatga cgtgccgccg ccgccgctgg gcgcggacag tccctacgat ttcaagggcg
     3661 accgccgcta tgcgggcgcg aacctgcccg atactgaaag cctgaaaacc acgctggacc
     3721 gcgtgctgcc gatgtgggaa ggcgagatcg cgccggagct gaaggcgggc aagaccgtgc
     3781 tggtcgccgc gcacggcaac tcgatccgcg ccatcctcaa atcgttgttc catctgtcgg
     3841 aacaggcgat cctcgacgtc gaggtgccga ccggcaatcc gctggtcatc cagttggata
     3901 acgacctgaa accggtgtcg gccaaatacc tcgacgccgc ccgcgccaat cccgtgccgg
     3961 tttatggggc gtaaagacgg aagaacccct caccctaacc ctctcccctc aagcggggag
     4021 aggggacagg gtcgtttaac ctcctctccc ctttcaaggg gagagggctg gggtgagggg
     4081 taaaccgtgg gccgccgccg ctcgcgtctg acgcccctgg cgcgcgactt gcgacagggc
     4141 gataatgtcg ccgaggcgcg tttatggctg gccctgcgtg gccgtcagtc cggtgggttg
     4201 aaattccgtc gccagtttcc gatcggcccc tatatcgccg atttcgcctg tcccgaacgg
     4261 cgtctggtag tcgagctgga tggcggccag catatcgata atccgcgcga tgttgtgcgc
     4321 gaccgctacc tcaacgaaaa cggctggtcg gtggcccggt ttagcgcggg cgacgttctg
     4381 agcgataaga gcattgtgct cgacactctc ctcgccatct gcgaggcgcg gatctatcag
     4441 ccggtcgagt cctatgagtt tcggttttat ccggcgaggt tggagatgat ggaggcgacc
     4501 cctcaccccg accctctccc cttgcaaggg gagagggagc aggatgtcat gtatatgcgc
     4561 cgcgccatcg aactggcgcg ggggcaactg gggcggacat ggcccaatcc gccggtcggg
     4621 tgcgtgctgg tgaaggacgg ctccgtgatc gccgaggccg ccaccggcga cggcgggcgg
     4681 ccgcatgccg aggaggcggc gctggccgtc gcgggcgcgg cggcgcgcgg ggcgacggcc
     4741 tatgtcacgc
//
LOCUS       DASVLL010000136         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_491773,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000136 DASVLL010000000
VERSION     DASVLL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491773"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF402_02245"
     CDS             <1..502
                     /locus_tag="VF402_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799141.1"
                     /translation="ESRPVPVSYLDAAAEAFTAGVVARPQAAPYTPLPAGIVHGASTV
                     AAEPAAEPANVVIRPQGDNLQRRVSSLAAMTPDSILAAVEQAGVGLEPRRLSAPQGKA
                     DDLSVISGVTPGAETELNALGIWHYWQIAGWGPEQVAWVANRIRPSDRIARENWMAQA
                     ARLAKL"
     gene            complement(709..1662)
                     /locus_tag="VF402_02250"
     CDS             complement(709..1662)
                     /locus_tag="VF402_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HEX7799142.1"
                     /translation="MSGELAIELSAVVVTVRDNEALVLCLNLPDGDGPAYALPSGPFA
                     PHAHRTFDLALRAFVREQTGFNIGYVEQLYSFGDDDREAVIPGMEAARIISLGYLALT
                     NDNTFRARINAAWAPFQRFFPWEDWRAQSADGRNPALEALRGPLAQWCDAAPTPEDAR
                     LRRRRVDVLFPSDFDDWNEERVLDRYELLYSARLIEEAQRKTPGAALPASGEPMASDH
                     RRILATALSRLRGKLKYRPIIFELTPPRFTLLELQKSLEALTGLRLHKQNFRRGLERT
                     GFVAPTGQMREDTGGRPAELYAYDRDRFRFSPSSGLSLPRA"
     gene            complement(1659..>2166)
                     /locus_tag="VF402_02255"
     CDS             complement(1659..>2166)
                     /locus_tag="VF402_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEX7799143.1"
                     /translation="DDIALGLSRVARWNGQTRGEHGFSVAQHSLIVEAICGHIQPELA
                     PSWRLMALLHDAPEYVIGDMISPFKAALGLDYRRFEDQLEKAIHIRFGLPAVMPAPVK
                     KLIKLADHACAFFEATQLVGFSDAEARRWFGQPPAGYHLVIAPLAPAAASAAFVARFE
                     ALLEQLNA"
BASE COUNT          354 a          735 c          748 g          329 t
ORIGIN      
        1 ggaaagccgt cccgtgccgg tcagctatct cgacgccgcc gccgaagcct tcaccgccgg
       61 cgtcgtcgcg cgtcctcagg ccgcgcccta tacgcccttg cccgcgggca tcgtgcatgg
      121 cgcatcgacg gtggcggcgg aaccggcggc ggaacccgca aacgtcgtga tccggccgca
      181 gggcgacaac ctgcaacgcc gcgtctccag tctggcggcc atgacgccgg acagcatcct
      241 tgcggcggtc gagcaggcgg gcgtcgggct ggagccgcgg cgcctgtccg cgccgcaggg
      301 caaggcggat gatctcagcg tcatttccgg cgtcacgccg ggcgcggaaa ccgaactgaa
      361 cgcgctgggc atatggcact attggcagat cgcgggctgg gggccggaac aggtcgcctg
      421 ggtcgccaac cgcatccgcc cctccgatcg catcgcgcgc gaaaactgga tggcgcaggc
      481 ggcgcggctg gcgaagctgt aggtttgggg gaaggttttt tgccacgaaa ggcacgaaag
      541 ggcacgaaat tcattgagac tggctttttc ggagacgtct cgacacgaat gagcgctttg
      601 cgctcgataa cctgtccggc tttctcaagg caaaagggct actcagcttt cgtgcccttt
      661 cgtgtttttc gtggcaaaac aactaccccc gtccgccagc ccgaaaactc acgcccgcgg
      721 caagctcaac cccgatgacg ggctgaagcg gaagcggtcg cggtcgtagg cgtaaagctc
      781 ggcggggcgt ccgccggtgt cctcgcgcat ctggccggtg ggcgcgacga agccggtgcg
      841 ctccagcccg cgccggaaat tctgcttgtg cagccgcagg ccggtcagcg cctccagcga
      901 cttctgcaat tccagcaggg tgaaacgcgg cggcgtcagc tcgaaaatga tcggccggta
      961 tttcagcttg ccccggagcc gcgacagggc ggtggccagg atgcggcggt ggtcggacgc
     1021 catcggctcg ccgctggcgg gcagggcggc gccgggcgtc ttgcgctgcg cctcctcgat
     1081 cagccgggcg ctgtacagca gttcgtagcg gtccagcacg cgctcctcgt tccagtcgtc
     1141 gaaatcgctg gggaacagca cgtcgacccg gcggcggcgc aggcgcgcgt cttcgggcgt
     1201 gggcgcggcg tcgcaccatt gcgccagcgg cccgcgcagc gcctccagcg cgggattgcg
     1261 cccgtccgcg ctctgggcgc gccagtcctc ccacgggaag aagcgctgaa acggcgccca
     1321 ggcggcgttg atgcgcgcgc ggaacgtgtt gtcgttggtc agcgccagat agcccagcga
     1381 gatgatgcgc gcggcctcca tgccgggaat gaccgcctcg cggtcgtcgt cgccgaacga
     1441 atagagctgc tcgacatagc cgatattgaa ccccgtctgt tcgcgcacga aagcccgcag
     1501 cgccaggtcg aaggtgcggt gcgcgtgcgg cgcgaacggc cccgaaggca gggcgtaggc
     1561 gggaccgtcc ccgtccggca ggttcaggca cagcaccagc gcctcgttgt cgcgcaccgt
     1621 cacgaccacc gccgacagtt cgatggccag ttccccgctc atgcgttcaa ctgctccagc
     1681 agagcctcga agcgcgccac gaaggcggcg gacgcggcgg cgggcgccag cggcgcgata
     1741 acgagatggt aaccggcggg cggctggccg aaccatcgcc gcgcctcggc gtcgctgaac
     1801 ccgaccagtt gcgtggcctc gaaaaaggcg caggcgtggt cggccagctt gatcagcttc
     1861 ttcaccggcg cgggcatgac ggcgggcagg ccgaagcgga tatggatcgc cttttccagt
     1921 tgatcctcga aacggcggta gtccagcccc agcgccgcct tgaacggcga gatcatgtcg
     1981 ccgatcacat attccggcgc gtcgtgcaaa agcgccatca gccgccacga cggcgccaac
     2041 tccggctgga tgtggccgca gatcgcctcg acgatcaggc tgtgctgcgc gacgctgaaa
     2101 ccgtgctcgc cgcgcgtctg gccgttccag cgcgccacgc gcgacaggcc cagcgcgatg
     2161 tcgtcg
//
LOCUS       DASVLL010000137         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_491862,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000137 DASVLL010000000
VERSION     DASVLL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491862"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VF402_02260"
     CDS             <1..1765
                     /locus_tag="VF402_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX7799144.1"
                     /translation="GGGVDYYVLTGATPRDIVEDYTWLTGRPPLTPEWALGFQQSHWS
                     YMTQAEAKGIADRLRADKIPSDAIWLDIDFQDKLRPFTVNTQTYTDLPKLVADLKAEN
                     MHLVVITDLHIAAAPNEGYAPYDDGMKKDIFVHRADGSTFVGPVWPGDAVFPDFSRPA
                     ARDWWGEQYKAFVGMGVSGFWNDMNEPSVFNTPTKTMPLDNVHRIEEPGFAPRNASHA
                     EMHNVFGMLNTEATYDGVLKLAPDRRPFVMTRASYAGGQKFAATWTGDNSSSWAHLQL
                     SVSQLVNLGLSGFAYAGDDIGGFAGDQPSAELLTRWIEVGAFNPIFRDHYQKDKPAQE
                     VWVNGPEQEAIRRRYIDERYRLMPYIYSLADENSRTGLPIMRPVFMDYPKVVAGGDHL
                     GGTDGQFMLGADLLIAPPATWESPAPYKVTLPGSGWYDYWSGEKLASDSTMETPKLDR
                     LPVFVRPGSIIPKQPLVQSTGETPVGPLTLEVYPGGDCRGFVYQDDGISFAYRSGHYL
                     RQSFTCKATATGLSFDFGAREGDVRPWWTGVTLLIHGQDAAATATLNGKALKAGFDAA
                     AHTLRVDLPDQSGPAAVIVGR"
     gene            complement(1773..>2643)
                     /locus_tag="VF402_02265"
     CDS             complement(1773..>2643)
                     /locus_tag="VF402_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7799145.1"
                     /translation="MAAAIGYPLAERLNALANGGAADGGATASAGEAQTVIEQATASI
                     AQVSRERFALSERMLKTALAADRDNVDLAVGLASLQLRGVQMNWYPPADRDKAVANAR
                     RVLEHTLRVQPASAPALTTYCRLLESTNDFADSLVACARALAYNPWDGTARTHIGMDQ
                     LELGRFDDAAASFRQADADGMPEVSRWVWKLDLGMTYLLMGRDAEALPWLNSSIAITP
                     ATGRSYIMLAVAYQGLGRTDEAHAAIARALALRPGTTVANLGFPTKNESPAYLAAAQR
                     VYRLAGAAGLPER"
BASE COUNT          419 a          906 c          909 g          409 t
ORIGIN      
        1 gggcggcggc gtcgactatt acgtcttaac cggcgcgacg ccgcgcgaca tcgtcgagga
       61 ctatacctgg ctgaccggca ggccgccgct gacccccgaa tgggcgctgg gcttccagca
      121 gtcgcactgg agctatatga cgcaggccga ggccaagggg atcgcggatc gcttgcgcgc
      181 cgacaagatc ccgtccgacg ccatctggct cgacatcgac tttcaggaca aactgcgccc
      241 gttcacggtc aacacgcaga cctataccga cctgccgaag ctggtcgcgg acctgaaggc
      301 cgagaacatg catctggtgg tgatcaccga cctgcatatc gccgccgcgc cgaacgaagg
      361 ctatgcgccc tatgacgacg gcatgaagaa ggacatcttc gtgcacaggg cggacggctc
      421 gaccttcgtg gggccggtct ggccgggcga cgcggtcttc ccggatttca gccgccccgc
      481 cgcgcgcgat tggtggggcg agcagtacaa ggccttcgtc gggatgggcg tgtcgggctt
      541 ctggaacgac atgaacgagc cgtcggtgtt caacacgccg accaagacca tgccgctcga
      601 caacgtccac cgcatcgagg agccgggctt tgcgccgcgc aacgccagcc acgccgagat
      661 gcacaatgtc ttcggcatgc tgaacaccga ggcgacctat gacggcgtgt tgaagctcgc
      721 cccggaccgg cgcccgttcg tgatgacgcg cgcctcctac gcgggcgggc agaagttcgc
      781 ggccacctgg accggcgata actcctcgtc gtgggcgcac ctgcaattgt cggtgtcgca
      841 actggtcaat ctgggcctgt cgggcttcgc ctatgcgggc gacgacatcg gcggcttcgc
      901 gggcgaccag ccctcggcgg agctgctgac gcgctggatc gaggtcgggg cgttcaaccc
      961 gatcttccgc gaccactatc agaaggacaa gcccgcgcag gaggtctggg tcaacggccc
     1021 tgagcaggag gcgatccgcc gccgctatat cgacgagcgc tatcgcctga tgccgtacat
     1081 ctacagcctg gccgacgaga acagccgcac cggcctgccg atcatgcgcc cggtgttcat
     1141 ggactacccc aaggtggtgg cgggcggcga tcatctcggc ggcacggacg gccagttcat
     1201 gctgggcgcc gacctgttga tcgcgccgcc ggcgacgtgg gaatcgcccg cgccctataa
     1261 ggtgaccttg cccggcagcg gctggtacga ctactggtcg ggcgagaaac tggcgtcgga
     1321 ctcgacgatg gagacgccga agctcgaccg cctgccggtg ttcgtgcgtc ccggttcgat
     1381 cattccgaag cagcctctgg tgcaatcgac cggcgagacg cccgtggggc cgctgactct
     1441 ggaggtctat ccgggcggcg actgccgcgg cttcgtctat caggacgacg ggatcagctt
     1501 cgcctacagg tccggccact atctgcgcca gagcttcacc tgcaaggcga ccgcgacggg
     1561 gctgagcttc gacttcggcg cgcgcgaagg cgacgtcagg ccgtggtgga ccggcgtgac
     1621 cctgctgatc cacggtcagg acgcggcggc cacggcgacg ctgaacggca aggcgctcaa
     1681 ggccggtttc gacgcggcgg cgcataccct gcgcgtcgac ctgcccgatc agagcgggcc
     1741 cgccgccgtg atcgtcgggc gctgagcggg cctcagcgtt cgggcaggcc cgccgccccg
     1801 gccagacgat agacgcgctg cgccgccgcc agataggcgg ggctctcgtt cttcgtcgga
     1861 aagcccagat tggccacggt cgtgccgggc cgcagggcca gcgccctggc gatggcggcg
     1921 tgcgcctcat ccgtgcggcc cagcccctga taggccacgg ccagcatgat gtagctgcgg
     1981 cccgtcgccg gggtgatggc gatcgagctg ttcagccagg gcagggcctc ggcgtcgcgt
     2041 cccatcagca ggtaggtcat ccccagatcc agcttccaca cccagcgcga aacctccggc
     2101 atgccgtcgg cgtcggcctg tcggaaggac gccgccgcgt cgtcgaaccg ccccagctcc
     2161 agctggtcca tgccgatatg cgtccgggcc gtgccgtccc acggattgta ggccagagcc
     2221 cgcgcgcagg ccaccagact gtcggcgaag tcgttggtcg attccagcag gcggcaatag
     2281 gtggtcaggg ccggcgcgct ggcgggctgg acgcgcaagg tatgctccag cacgcgccgg
     2341 gcgttggcca cggccttgtc gcggtcggcg ggcgggtacc agttcatctg cacgccgcgc
     2401 agttgcaggc tggccagacc gacggccaga tcgacattgt cgcggtcggc ggccagcgcg
     2461 gtcttcagca tccgttccga cagggcgaag cgttcgcgcg acacctgcgc gatggaggcg
     2521 gtggcctgtt cgatcaccgt ctgcgcctcg cccgccgagg cggtcgcgcc gccgtccgcc
     2581 gcgccgccgt tggccagcgc gttgaggcgc tccgccagcg gatagccgat cgccgccgcc
     2641 atc
//
LOCUS       DASVLL010000138         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_383838,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000138 DASVLL010000000
VERSION     DASVLL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383838"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF402_02270"
     CDS             <1..216
                     /locus_tag="VF402_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799146.1"
                     /translation="LLLTDIVMPGADGIEVAKRAAERQPELKIMFITGFAAVALNAKK
                     ASPNAKVLEKPVHLREIVTEVERLIAA"
     gene            complement(395..469)
                     /locus_tag="VF402_02275"
     tRNA            complement(395..469)
                     /locus_tag="VF402_02275"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Val,seq:gac)
     gene            complement(569..1480)
                     /locus_tag="VF402_02280"
     CDS             complement(569..1480)
                     /locus_tag="VF402_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7799147.1"
                     /translation="MILFAAAALMVGAAPAFALQDAVALTDQQRTPETMDIGVLTQKA
                     QAGDARAQVRLGDDYYVGNGVGQDYAQAFRWYDKAARAGDPEAQTELGLMYAKGQGVA
                     KDYAQALSWTRKAADQGYAGAQYNMGIAYDTGVAVAHDAAAAFGWYLKAAKQGYDIAQ
                     FNVANMYELGDGVAQDYAQARAWYQKAVVQGYAPAQYNLGNMDLAGEGAPADAAKALP
                     LIAASAKQGFPLAQSRMAALYYQGDGVAQDKVTAYAWALLAADAGDGDAQALLAAMDS
                     GHELSDDQLARAARTADQLAQSLTGGA"
     gene            complement(1678..>2528)
                     /locus_tag="VF402_02285"
     CDS             complement(1678..>2528)
                     /locus_tag="VF402_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HEX7799148.1"
                     /translation="FSGWLSAAAGVKGFFRPWFLPPWFNPPGTPEATSFYDTAPLRET
                     LLRHVDFDRINKGPLRLSLGAVQVRTGNFVYFDTQEPERVIGPEHVMASAALPPGFPA
                     VHVDGEAYWDGGLVSNTPLSYVLDAGVAKDTLVFQIDLFSAVGPVPQTMDEVQERIKD
                     ITYSSRTRLNTDAFLEKYRLRHAIRALAQYVPEDALERLCDQSLPADVYDGRVSLVHI
                     INRSNRREIQSKDFEFWRVSLDQHWRDGMRDARTAMDAESWREFSDPDTGLAVYDYMR
                     SDNGDD"
BASE COUNT          414 a          853 c          817 g          444 t
ORIGIN      
        1 ctgttgctga ccgacatcgt catgcccggc gccgacggca tcgaggtcgc caagcgcgcc
       61 gccgagcgcc agccggaact gaagatcatg ttcatcaccg gcttcgccgc cgtggcgctc
      121 aacgccaaaa aagcctcgcc caacgccaag gtgctggaaa agcccgtgca tttgcgcgaa
      181 atcgtcaccg aagtcgaacg cctgatcgcg gcgtaaagcg cgcaccggat tgggggctgt
      241 cgcaaacaga attcgccagc cgctttcacg tcccggtcgg cacgctgcgc gactgggaac
      301 aggcccgcgt caccgcgccc gatttcgccg tcgcctgacg ggcggcgcgc aacaaaaaag
      361 cgtccggcga accggacgct tttttgtgtg aagatggtgg gcgctacagg gattgaacct
      421 gtgaccccta ccatgtcaag gtagtgctct cccgctgagc taagcgccca tcttcatctc
      481 gatcccgacg tcgccgccga ggagcgggtg tatagccaag ccccgccgcc gtggcaagcg
      541 ctcaggagat ttttctgcgg aaaaaagatc aggccccgcc ggtcaggctt tgcgccagtt
      601 gatcggcggt ccgggcggcg cgtgcgagct ggtcgtcgct cagttcgtgg ccgctgtcca
      661 tcgccgccag cagcgcctgc gcgtcgccgt cgcccgcgtc cgccgccagc aaggcccagg
      721 cgtaggccgt gaccttgtcc tgcgccacgc cgtcgccctg atagtagagc gcggccatgc
      781 ggctttgcgc cagcgggaag ccctgcttcg ccgaggccgc gatcagcggc agcgccttcg
      841 cggcgtccgc gggcgcgccc tcgcccgcca ggtccatatt gccgagattg tactgcgccg
      901 gggcgtagcc ctgaacgacg gccttttgat accaggcgcg ggcctgcgcg taatcctgcg
      961 ccacgccgtc gcccagttcg tacatgttgg cgacgttgaa ctgggcgatg tcgtagccct
     1021 gcttcgccgc cttgaggtac cagccgaacg ccgccgccgc gtcgtgcgcg accgctacgc
     1081 cggtgtcgta ggcgatgccc atattgtact gcgcgcccgc atagccctga tcggcggcct
     1141 tgcgcgtcca gctcagcgcc tgcgcatagt ccttcgcgac gccctgcccc ttggcgtaca
     1201 tcaggcccag ctcggtctgc gcctccggat cgcccgcgcg cgccgccttg tcgtaccagc
     1261 ggaacgcctg cgcgtaatcc tgacccacgc cgttgccgac atagtagtcg tcgcccagcc
     1321 gcacctgcgc gcgcgcgtcg ccggcctgcg ccttttgcgt cagcacgccg atgtccatgg
     1381 tttcgggcgt gcgctgctgg tcggtcagcg ccaccgcgtc ctgcaaggcg aaggcgggcg
     1441 cggcgccgac catgagcgcg gcggccgcaa ataagatcat gatccgccgc atggcgacag
     1501 ctcccggagg gtcagaaaaa aagaaaaccg tactgttcgg ttttattata tgtgacgcct
     1561 gccggattcc gccagtagcg cggcccgaat ttcgtcattt ttggaaaaat taggtgattt
     1621 ggccacagcg gtgtgttcgc cgcgcaacat taatcgtcgc ccattaatcg tcgcccatca
     1681 atcgtcgcca ttgtcgctgc gcatatagtc atagaccgcc agcccggtgt cggggtcgct
     1741 gaattcgcgc caggattcgg cgtccatcgc ggtgcgggcg tcgcgcatgc cgtcgcgcca
     1801 gtgctgatcg agcgacacgc gccagaactc gaaatccttg gactggatct cgcgccggtt
     1861 gctgcggttg atgatgtgga cgaggctgac gcgcccgtca tagacgtcgg cgggcaggct
     1921 ctggtcgcac aggcgctcca gcgcgtcttc cggcacatat tgcgccagcg cgcgaatggc
     1981 gtggcgcagc cggtacttct ccagaaaggc gtcggtgttc agccgggtgc ggctggaata
     2041 ggtgatgtcc ttgatgcgct cctgcacctc gtccatggtc tgcggcacgg ggccgaccgc
     2101 gctgaacagg tcgatctgga agaccagcgt gtctttcgcc acgcccgcgt ccagcacgta
     2161 ggatagcggc gtgttggaga cgaggccgcc gtcccagtag gcttcgccgt cgacatggac
     2221 cgccggaaaa ccgggcggca gggcggcgga ggccatgaca tgctccggcc cgatgacgcg
     2281 ctccggctcc tgcgtgtcga aatagacgaa attgccggtg cgcacctgca cggcgccgag
     2341 gctcaggcgc agaggcccct tgttgatgcg gtcgaaatcg acatggcgca gcagcgtctc
     2401 gcgcaagggg gcggtgtcgt agaagctggt cgcttccggc gttccgggcg gattgaacca
     2461 cggcggcagg aaccacgggc ggaaaaagcc cttcaccccc gccgccgcgc tcagccagcc
     2521 gctgaact
//
LOCUS       DASVLL010000139         5588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_277496,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000139 DASVLL010000000
VERSION     DASVLL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277496"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..697)
                     /locus_tag="VF402_02290"
     CDS             complement(269..697)
                     /locus_tag="VF402_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7799149.1"
                     /translation="MLVNQLLNAKGRQVFTVTPDDALSSVAALLYTRKVGAFIVTDRI
                     GRVVGIVSERDIIRAIATQGAEALTRSVSHIMTRDVISAGAHETVEALLDRMTDRRIR
                     HLPVMEGARLIGIVSIGDLVKARIAASDHEARTLKEYIAG"
     gene            complement(808..1500)
                     /locus_tag="VF402_02295"
     CDS             complement(808..1500)
                     /locus_tag="VF402_02295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7799150.1"
                     /translation="MTDDPRSQPETPAREPVFNAPFLTLLLPAALIGLYALQASLSPY
                     MQDQVIESFGLSPVLLRQGHYDLLVTHLFLHGSWMHVLANAAFCLAFSAPVLRACGRG
                     PIGALSFLCYFLIGGAVAGAGDCLLNWSSAVPILGASGAVSALMGAAIRIRAWPGHPG
                     RLAPFTDARVISMTVFWCGVNAVAAFVPMLMGVAEGQGIAWQAHVIGYLFGLCSIGAW
                     LRLTNARFFTTS"
     gene            1728..2207
                     /gene="hfaA"
                     /locus_tag="VF402_02300"
     CDS             1728..2207
                     /gene="hfaA"
                     /locus_tag="VF402_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaA"
                     /protein_id="HEX7799151.1"
                     /translation="MSLLKPLIWLPLLTAAAYAGAAQAQFSTSSTVASFETGYGLARN
                     EMQTAINPSMRDANGNLVLQDGMIVTGADNSVYAYSKTLGAGDSYSGAGALGGATAIG
                     NNLQVVVNGNNNTVVVHANQTNNGNVTATANGTAVTGATGSASTDSSLNGQVNLNGF"
     gene            2197..3318
                     /gene="hfaB"
                     /locus_tag="VF402_02305"
     CDS             2197..3318
                     /gene="hfaB"
                     /locus_tag="VF402_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaB"
                     /protein_id="HEX7799152.1"
                     /translation="MASEIDSKTAKAAPASGGHSRKALVGLIGAAALSLGLSGCMTPG
                     VQADGNYKTPIGAAPVTANPTAYSDALVCLGAYARDHHLASPRLAVGRISDYTGKVSL
                     EGGREITQGASLMAMTALAKAGARQVERFDTSVGELELKYANNKLITDDAVANPTHPA
                     DYRRIMAGQVAGSDFFIVGGITELNYNIQSSGLDAYASDTKNTGLGLFSGRTYVMNVA
                     LDLRLVDTRTLEVVDVISYQKQIVGREVKAGVYSIMNGNLFDIDGGKGALEPMQLAVR
                     AMIERATLEFMANLYGVSGPGVCLDPRNDFLENPTLGPTGGLTPAYNNLETNNAQTRA
                     DPNRWTDVVSNRRDAPPPPADDRRNGADRRDAAVNDSRY"
     gene            3296..4483
                     /gene="hfaD"
                     /locus_tag="VF402_02310"
     CDS             3296..4483
                     /gene="hfaD"
                     /locus_tag="VF402_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchor protein HfaD"
                     /protein_id="HEX7799153.1"
                     /translation="MSTTPATSQTVGSAVSNNQDQQGDVLSSQTLNVVENDGLTSATG
                     QATGNALQGGNQDVDATLHSVQNVAGSIRAAANINGVNTGDEDTSLGTPVYGNTQAIG
                     NYMASTADQASLTATAAQTASGPVQAATEITAPNNAIYVSGEGDATAEANHAAFEVEN
                     GRLTSSMTQTSTSDVRADAYAQVHYSPSPNGYYAYATNNYYGAYSGDRGTQEHDVTQS
                     QTGTTTARAEINGGNVWQSDVEATTTGNNIDLQNQGGSLIVAANQTQSGQVTAQAKNT
                     ADQYSEAYATASGVGNNLAAGNNDVYLRLDTTQVSSGGVDVSAEFDGNSGYDGYVAAD
                     AVGNQALAYACSDCQADYGVNSTQVNTSDVNATATASVVQGRSIVSTARAVGNSATYY
                     VSH"
     gene            4717..>5588
                     /locus_tag="VF402_02315"
     CDS             4717..>5588
                     /locus_tag="VF402_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7799154.1"
                     /translation="MIFKKSLLTAFAVAALTSTALTPAAALAQTAAAAPAFTPQVRDL
                     SKGLDFPQNHSDIKADPNVRFGKLANGMTYIVMKNNTPPGTLSIRLRINGGSMMEHDD
                     QQGLAHFLEHMAFNGSKNVAEGDMVKILERHGLAFGADTNAHTGFDETVYELDLPTVK
                     PDDIDTGLFLMRETAGNLTLDDGAIDRERGVILGEERSRDTPGLHAYEKWADDAFRGQ
                     VYPTRLPIGKTDIIQTAKHDRFADFYTNFYRPELATLVVVGDADPDVIEADIKAKFSD
                     WKAPAAPIRATDFGA"
BASE COUNT         1109 a         1926 c         1713 g          840 t
ORIGIN      
        1 aaaacaaaat cctgccgggc tatctttgcg ggatagcctg ccaaggcttc gtgcgaatcg
       61 gaaggtcgct ttcataactc cgaacagcgc caacatcaag ccatgaattg aatttttata
      121 tccaatcgcc agctcgttgt cggcccgtcc ggtgcgcgtc agcgaggagg cggcttttat
      181 gccccctcac cggacggcgc aagcgcaaaa tgcagggaat gcgattctat ccccagcttc
      241 ggccccagct tcagccccaa cttcgggcct aacccgcgat atattccttg agcgtccgcg
      301 cctcatgatc cgaggcggcg atgcgcgcct tgaccagatc gccgatggag acgatgccga
      361 tcagccgcgc cccttccatg accggcagat ggcggatgcg ccggtcggtc atgcggtcca
      421 gcagggcttc gacggtttcg tgcgcgccgg cggaaatgac gtcgcgcgtc atgatatggc
      481 tgacgctccg ggtcagcgcc tcggcgccct gagtcgcgat ggcgcggatg atgtcgcgtt
      541 ccgaaacgat gccgacgacg cgcccgatgc ggtcggtgac gatgaaggcg ccgaccttgc
      601 gcgtatagag cagcgccgcg acggacgaca aagcgtcgtc gggcgtgacg gtaaagacct
      661 gacggccctt ggcgttgaga agctgattga ccagcatgga cgcaccctcc ctatgggtat
      721 tccggattat aaacgccgga taacgaggtt cgcgccgatg ccgtaaaaat gcaataagcc
      781 ggacaacttc atgcctgcgc cgcggcgtca acttgtcgtg aagaagcgcg cgttcgtcag
      841 gcgcagccac gcgccgatgc tgcacaggcc gaacagatag ccgatcacat gcgcctgcca
      901 ggcgatgccc tgcccctcgg cgacgcccat cagcatgggg acaaaggcgg cgaccgcgtt
      961 cacgccgcac cagaagacgg tcatgctgat cacgcgcgcg tcggtgaagg gcgccagccg
     1021 tcccggatgg ccgggccagg cgcggatgcg gatcgccgcg cccatcaggg ccgacaccgc
     1081 ccccgaggcg cccaggatcg gcacggcgga cgaccagttg agcaggcagt cgcccgctcc
     1141 cgcgaccgcg ccgccgatca ggaaatagca caggaaggac agggcgccga tcgggccgcg
     1201 tccgcacgcc cgcagcaccg gcgccgaaaa ggccaggcag aaggcggcgt tggccagcac
     1261 atgcatccag ctgccgtgca ggaacagatg cgtgaccagc aggtcgtaat gcccctgccg
     1321 cagcagcacc ggcgacaggc cgaagctttc gatcacctga tcctgcatat aaggactgag
     1381 cgaggcttgc agcgcataaa gcccgatcag cgccgccggc agcagcagcg tcaggaacgg
     1441 cgcgttgaac accggctcgc gcgcgggcgt ttcgggctgg gagcggggat catctgtcat
     1501 atttcgggac tcgggccgga tcgatttgag cctatatgcc ctttcgagcc cgcgaagaac
     1561 agggccgcgc cgccgcaagg cgctgtaaaa agcgcggaaa tatgaaattc ctgcgccaaa
     1621 tcgggaaaaa tatacctcat ctttatgctt gtcgcccaga ttggcatcca atttgctgga
     1681 cctgcgccat gcctgcggcg ccgcgcccgc aggcctcgga gcgcacgatg tccctgttaa
     1741 agcccctgat ctggctgccc ctgctgaccg ccgccgccta tgccggcgcc gcgcaggcgc
     1801 aattcagcac cagcagcacc gtcgcctcgt tcgagaccgg ctacgggctg gcgcgcaacg
     1861 agatgcagac ggcgatcaat ccgtccatgc gcgacgccaa cggcaatctc gtcttacagg
     1921 acggcatgat cgtcaccggc gccgacaaca gcgtctacgc ctattccaag acgctgggcg
     1981 cgggcgattc ctattccggc gccggcgcgc tgggcggcgc gaccgcgatc ggcaacaatc
     2041 ttcaggtcgt cgtcaacggc aacaacaaca ccgtggtcgt tcacgcgaac cagaccaata
     2101 acggcaacgt caccgccacc gccaacggaa ccgccgtcac cggcgcgacc ggttcggcat
     2161 ccacggactc ttctcttaac ggacaggtaa acctcaatgg cttctgagat cgacagcaag
     2221 accgcgaagg cggccccggc gtcgggcggg cattcgcgca aggccctggt cggtctgatc
     2281 ggcgccgcgg ccctgagcct gggcttgtcg ggctgcatga cccccggcgt tcaggccgac
     2341 ggcaattaca agacgccgat cggcgccgcg ccggtcaccg ccaatccgac cgcctattcg
     2401 gacgcgctgg tctgtctggg cgcctacgcc cgcgatcacc acctcgcctc gccgcgtctg
     2461 gcggtgggcc gcatctccga ctacaccggc aaggtgtcgc tggaaggcgg gcgcgaaatc
     2521 acgcagggcg cgtcgctgat ggcgatgacg gccctggcca aggcgggcgc ccgtcaggtc
     2581 gagcgcttcg acaccagcgt cggcgagctt gagctgaaat acgccaacaa caagctgatc
     2641 accgacgacg ccgtggccaa tccgacccat ccggcggact accgcaggat catggccggt
     2701 caggtcgccg gttccgactt cttcatcgtc ggcggcatca ccgagttgaa ctacaacatc
     2761 cagtcgtccg gcctcgacgc ctatgcgtcc gacacgaaga acaccggcct cggcctgttc
     2821 tccggccgca cctatgtgat gaacgtcgcg ctggacctgc ggctggtcga cacccgcacg
     2881 ctcgaggtcg tggacgtcat ctcctatcag aagcagatcg tcggccgcga agtcaaagcc
     2941 ggcgtttaca gcatcatgaa cggcaacctg ttcgacatcg acggcggcaa gggcgcgctg
     3001 gagccgatgc aactggcggt gcgcgcgatg atcgagcgcg cgacgctgga gttcatggcc
     3061 aatctgtacg gcgtgtccgg ccccggcgtc tgcctcgacc cgcgcaacga cttccttgaa
     3121 aatccgaccc tcggcccgac gggcggcctg acgccggcct ataataatct ggagacgaac
     3181 aatgctcaga cgcgcgctga tccgaaccgc tggacggacg tcgtgtccaa ccgccgcgac
     3241 gcgcctccgc ctccggctga cgaccgccgc aacggcgctg atcgccgcga cgctgctgtc
     3301 aacgactccc gctactagcc agaccgtcgg cagcgccgtc agcaacaacc aggaccagca
     3361 gggcgacgtc ctgtcgagcc agacgctgaa cgtcgtcgag aacgacggcc tgacctcggc
     3421 gacgggtcag gcgaccggca atgcgttgca aggcggcaat caggacgtcg acgccacgct
     3481 gcattcggtg cagaacgtgg ccggttcgat ccgcgccgcg gcgaacatca acggcgtcaa
     3541 caccggcgac gaggacacct cgctgggcac gccggtttac ggtaacacgc aggccatcgg
     3601 caactacatg gcctcgaccg cggatcaggc cagcctgacc gcgacggcgg cccagaccgc
     3661 cagcgggccg gtgcaggcgg cgaccgaaat caccgcgccg aacaacgcca tctatgtcag
     3721 cggcgaaggc gacgccacgg ccgaggccaa ccacgccgcc ttcgaggtcg aaaacggcag
     3781 gctgacctca agcatgaccc agacctcgac cagcgacgtg cgggccgacg cctacgcgca
     3841 ggtgcattat tcgccctcgc ccaacggcta ttacgcctac gcgaccaaca actattacgg
     3901 cgcctacagc ggcgatcgcg gcacgcagga gcacgacgtc acgcaaagcc agaccggaac
     3961 gacgacggcg cgcgccgaaa tcaacggcgg caatgtctgg cagtcggacg tcgaggcgac
     4021 cacgaccggc aataatatcg acctgcaaaa tcagggcggc tccctgatcg tcgccgccaa
     4081 ccagacccag tcgggccagg tgaccgcgca ggcgaagaac accgccgacc agtacagcga
     4141 agcctacgcc accgcgtcgg gcgtcggcaa caatctggcc gcgggcaata acgacgtcta
     4201 tctgcgcctc gatacgactc aggtctcgtc gggcggcgtc gacgtcagcg ccgaattcga
     4261 cggcaacagc ggctatgacg gctatgtcgc cgccgacgcg gtcggcaatc aggcgctggc
     4321 ctacgcctgc tccgactgtc aggccgatta cggcgtgaac tcgacgcagg tgaacacttc
     4381 cgacgtcaac gccaccgcga ccgccagcgt cgtccagggc cgctccatcg tctccaccgc
     4441 ccgcgcggtc ggcaacagcg cgacctatta cgtatcgcac taaacgccgc cctggcggac
     4501 gcctcctttt tatacctccc cgatttatat ctccctgaac gcacagcgtt cggggagagg
     4561 gagcgcacga ggccgcgaag cggctgagaa gtggcggcgg ggcgtcttat cttcccccct
     4621 ccgcaacctt aacaaaagtt aatcctgcaa cttctcgctt tattcgcccc ccgctttgcg
     4681 ccagtgtcgc gcgcacatgc taccgggagt ctgatcatga tcttcaaaaa gtcccttctg
     4741 accgccttcg cggtcgccgc cctgacctcg accgcgctga cgccggcagc ggcgctggcg
     4801 cagacggcgg cggcggcgcc cgccttcacg ccgcaggtcc gcgacctgtc gaaggggctg
     4861 gacttcccgc agaaccattc cgacatcaag gccgatccga acgtgcgctt cggcaagctg
     4921 gccaacggca tgacctatat cgtcatgaag aacaacacgc cgccgggcac tttgtcgatc
     4981 cggctgcgca tcaacggcgg ctcgatgatg gagcatgacg accagcaggg tctggcgcac
     5041 ttcctcgaac acatggcgtt caacggctcg aagaacgtgg ccgagggcga catggtgaag
     5101 attctggagc gccacggcct cgccttcggc gccgacacca acgcccacac cggcttcgac
     5161 gaaaccgtct atgagctgga cctgccgacg gtgaagcccg acgacatcga caccggcctg
     5221 ttcctgatgc gcgagacggc gggcaatctg acgctggacg acggcgccat cgaccgcgag
     5281 cgcggcgtca ttctgggcga agagcgctca cgcgacacgc ccggcctgca cgcctatgaa
     5341 aaatgggcgg acgacgcctt caggggacag gtctatccga cccgcctgcc gatcggcaag
     5401 accgacatca tccagaccgc gaagcacgac cgcttcgccg acttctacac gaatttctac
     5461 cgcccggagc tggcgacgct ggtcgtggtc ggcgacgccg atcccgacgt tatcgaagcc
     5521 gacatcaagg cgaaattctc cgactggaaa gcgcccgcgg cgccgatccg cgcgaccgat
     5581 ttcggcgc
//
LOCUS       DASVLL010000140         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_115771,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000140 DASVLL010000000
VERSION     DASVLL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115771"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            546..713
                     /gene="rpmG"
                     /locus_tag="VF402_02320"
     CDS             546..713
                     /gene="rpmG"
                     /locus_tag="VF402_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7799155.1"
                     /translation="MAKPASIKIRLNSTADTGFFYVTKKNARTKTEKMVLKKYDPVVR
                     KHVEFREGKIK"
     gene            844..1293
                     /locus_tag="VF402_02325"
     CDS             844..1293
                     /locus_tag="VF402_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799156.1"
                     /translation="MPRLPILVLTGLLLAAPAIAQTNPATPNPAADAPPAGAPTLPQA
                     ISSLLSITYACRGVNGPALYDQADDMARRLTQRVTGDANLTVQFMKRAEDQARAACPN
                     TDSCWRELMDAGTPVTTENAKAACDRLSGQAAQTVADAYAALTGRKD"
     gene            complement(1396..>1775)
                     /locus_tag="VF402_02330"
     CDS             complement(1396..>1775)
                     /locus_tag="VF402_02330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7799157.1"
                     /translation="HFKAVNDGYGHDAGDEALREFARRLGLHVRAIDLPCRLGGEEFV
                     VLMPETGAGDALNIAERVRAQIAAEPFAVGGGRLLNVTVSVGVATSGGLGDSPEALLK
                     RADEGVYEAKSSGRNRVILRSAA"
BASE COUNT          360 a          590 c          506 g          319 t
ORIGIN      
        1 gtgaaaggaa acgaccctac tttccgacgg ttcttggaac ccaacacgaa acggtccgta
       61 tccttccgtc gtgactgggg tggctgcccc ggtcacgacc gttcatctct agccaaaatg
      121 aacacgcaaa gcatgagaca aacacggatg atggccttcg gccatctgca aggacaaggg
      181 aaggcgctgc gctttccgcg ctctcatttt tgtctgtgta cgccgcgaca gcggcgatcc
      241 gtgaagatgg ccgaaggcca tcatctgtgc tttccgtggt tcaatccctt aaatggtcgt
      301 taaatgccga tccaacccag tcgagcgccg cccggacagc ttacgcgaac catctgtgct
      361 tatctgtctg atctgtggat aaaatccttc cttctcttta gctcgcgata aaaagaaaaa
      421 ggccccataa cccttgactt cctgcgcttg gtgagtattt tccgcgcctc tgaattattt
      481 gcgcgccatg acggagtctt tgggacacgg tctttgggcg tcacgaaacg caaggacttt
      541 aggtcatggc taagcccgcc tccatcaaga tccgtctgaa ctcgacggcc gacaccggct
      601 tcttctacgt caccaagaag aacgcccgca ccaagaccga aaagatggtg ctgaagaagt
      661 acgatccggt cgtgcgcaag cacgttgaat tccgcgaagg caagatcaag taatctcgct
      721 tttgcaacac ctttgcccgc tggctccccc ccccctcgcc ggcggacaat cgaagccgcg
      781 cctgagttcc ccccaaagaa atctcggcgc ggctttttca tgtcccgacg ccggagatcc
      841 gccatgccgc gcttgccgat tctggttctg acgggcctgc tgctggcggc gcccgcgatc
      901 gcccagacaa accccgcgac gcccaaccct gcggctgatg cgcccccggc aggcgcgccg
      961 accctgccgc aggcgatctc cagcctgctc agcatcacct acgcctgccg cggcgtcaac
     1021 ggcccggcgc tttacgatca ggccgacgac atggcgcgcc gcctgaccca gcgcgtcacg
     1081 ggcgacgcca acctcaccgt gcagttcatg aaacgcgccg aggatcaggc cagagccgcc
     1141 tgccccaaca ccgattcgtg ctggcgcgag ctgatggacg cgggaacccc cgtcacgacg
     1201 gaaaacgcca aggccgcctg cgacaggctc agcggacagg ccgcccagac ggtcgccgac
     1261 gcctacgcgg cgctgacggg caggaaggat tagagcgttt ccccaaaaag tggtcccact
     1321 ttttggataa ggaagagcga caaaacaaag acttagagcg cttttcgatc tgattccatc
     1381 agatcgaaaa gcgctctaag ccgccgagcg caggatgacg cggttgcggc ctgaggattt
     1441 agcctcatag acgccttcat cggcgcgttt cagcaaggct tccgggctgt cgccgaggcc
     1501 gcccgaggtc gcgacgccga cggacacggt gacgttgagc aagcgcccgc cgccgacggc
     1561 gaacggttcg gcggcgatct gggcgcggac ccgttcggcg atgttcagcg cgtccccggc
     1621 gccggtttcc ggcatcagca ccacgaactc ctcgccgccc agacggcacg gcaggtcgat
     1681 ggcgcgcaca tggaggccga gccgccgggc gaattcgcgc agcgcctcgt cgcccgcgtc
     1741 gtggccatag ccgtcattga ccgccttgaa gtgat
//
LOCUS       DASVLL010000141         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_383980,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000141 DASVLL010000000
VERSION     DASVLL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383980"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="VF402_02335"
     CDS             complement(<1..1767)
                     /locus_tag="VF402_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX7799158.1"
                     /translation="MQLLGQNVSPPHLNDLLLGKTVKRPVLRGVALFKRHLQGSEVWF
                     IVLATGVGLLAGVMAALLSHTAHLIQSILYGFSPDLRLSALPSLQPWHLLALPMGGFV
                     LGVLRLIFPRRSRQPIDVIEANALYGGRIPLRDSLMVSLQTLISNGFGASVGLEAAYA
                     QMGGGMASKIGQWLDLKRASLRTLVGAGAGAAIGAAFNAPLAGAFYAFEIVLGAYTPA
                     ALAPVAAASLTAVVAAQLMQNEPFVIASTIGRDISLPGYLLFALLGIICAGVGIMIIR
                     LVAAAESFVRRLPIGDVWRPLVGGICLMPVAFVAPQALSSGHGAMQLFLAQQSLILTL
                     LAVFVLKMCGSVLSLAFGFRGGLFFASLFLGSLLGAAFAMIVNHIWPAANLDPTNSAL
                     VGMAGLAVGIVGGPMTMSLLVLEVTHDFALTAAVVTAALCCSAIMREYFGYSFSTWRL
                     HLRGEAIRTPRDIGWVRALTAARLMRKNHTALPDTISIAALREQVPLGSTSRVLLVDA
                     AGDYRGLVLTAEAYGESVDVDAPASRIARQTDVYVRPDMDIAAIQRVFEHFGVDDLAV
                     VSEDHKLMGILTEKYVTRRYAEE"
BASE COUNT          373 a          656 c          658 g          260 t
ORIGIN      
        1 ctcctcggcg tagcgccggg tgacgtattt ttccgtcaat atgcccatca gcttgtggtc
       61 ctcgctgacc acggccagat cgtcgacgcc gaaatgctcg aacacgcgct ggatggcggc
      121 gatgtccatg tccgggcgca catagacatc ggtctggcgc gcgatccggc tggccggggc
      181 gtccacatcg acgctttcgc cgtaagcctc ggcggtcagg acgaggccgc gataatcgcc
      241 cgccgcgtcg accagcagca cgcggctggt cgaacccagc ggcacctgct cgcgcaaggc
      301 ggcgatgctg atcgtgtcag gcagcgcggt atggttcttg cgcatcagcc gcgcggcggt
      361 cagcgcgcgc acccagccga tgtcgcgcgg cgtgcggatc gcctcgccgc gcaggtgcag
      421 ccgccaggtc gagaaggaat agccgaaata ttcgcgcatg atggccgagc agcacagcgc
      481 cgccgtcacc accgccgccg tcagcgcgaa gtcgtgcgtg acctccagca ccagcagcga
      541 catggtcatc ggcccgccga cgatgccgac cgccagcccc gccatgccga ccagcgccga
      601 attggtcgga tcgagattgg ccgcgggcca gatatggttg acgatcatgg cgaaggccgc
      661 gcccagcagc gagccgagaa acagcgaggc gaagaacagc ccgccgcgaa acccgaaggc
      721 cagcgacagc accgagccgc acatcttcag cacgaacacc gccaaaaggg tcaggatcag
      781 cgattgctgc gccaggaaca actgcatggc gccgtggccg gacgacaggg cctgcggcgc
      841 gacgaaggcg accggcatca ggcatatgcc gccgaccaga ggccgccaca cgtcgcctat
      901 gggcaggcgg cgcacgaagg attcggcggc ggccaccagc cggatgatca tgatgccgac
      961 cccggcgcag atgatgccca gcagcgcgaa caacagatag ccgggcagcg agatgtcgcg
     1021 tccaatggtg ctggcgatga cgaacggctc gttctgcatc agttgcgcgg cgaccaccgc
     1081 cgtcagcgac gccgccgcca ccggcgccag cgcggcgggc gtataggcgc ccagcacgat
     1141 ctcgaacgca tagaaggcgc ccgccagcgg cgcgttgaac gccgcgccga tggccgcccc
     1201 ggccccggcc ccgaccaggg tgcgtaaaga cgcgcgcttc aggtccagcc actggccgat
     1261 cttggacgcc atgccgccgc ccatctgggc gtaggcggcc tcaagcccca ccgaggcgcc
     1321 gaaaccgttg gagatcaggg tttgcagcga caccatcagg ctgtcgcgta aggggatgcg
     1381 cccgccgtac agcgcattgg cctcgatcac gtcgatcggc tggcgcgagc ggcgcggaaa
     1441 gatcagccgc agcaccccca gcacgaagcc gcccatcggc agggccagca gatgccacgg
     1501 ctgcaaggac ggcagggcgc tcaggcgcag gtcgggcgag aagccgtaca ggatcgactg
     1561 gatcaggtgc gcggtatggc tgagcagcgc cgccatcacc ccggccagca gaccgacgcc
     1621 ggtggccagc acgatgaacc agacttccga accttgcaga tggcgcttga acagcgcgac
     1681 gccgcgcagc accggacgct tgaccgtctt gccgagcaga aggtcgttga gatgcggcgg
     1741 gctgacgttc tggccgagca gttgcatcaa gggctccggt ccggcgaagc ggcggcgcga
     1801 agggaagatt cctcaataaa cccttagact tctcctgccg cgatgtgaag ccgcttcgcg
     1861 cgccgggcgg cgattttatc gctgacggca gccggggctt tggggaaggc gctaggctgt
     1921 agtgacaaat tagggattcc cgttttg
//
LOCUS       DASVLL010000142         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_277600,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000142 DASVLL010000000
VERSION     DASVLL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277600"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..488)
                     /locus_tag="VF402_02340"
     CDS             complement(3..488)
                     /locus_tag="VF402_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7799159.1"
                     /translation="MARAPRIRRIGPEELEIVRQLALIIWPKCYRNIIGPDRVDAMLA
                     VLYATEALETEMRGGHVFWVARVGDLDVGYASAWLDGERLWLKKLYVRDDFRGHGLGK
                     ALIDTALGHFPARELALNVNKDNAPAIAYYLRSGFTQEAETPVRMGPYEFTDYVMRKA
                     L"
     gene            complement(583..1680)
                     /gene="ald"
                     /locus_tag="VF402_02345"
     CDS             complement(583..1680)
                     /gene="ald"
                     /locus_tag="VF402_02345"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX7799160.1"
                     /translation="MKISVPREIKNHEYRVGLVPHAVAELTHHGHRVFVETQAGAGVG
                     FSDDDYRAAGAAILPTPEAVFDAGEMIVKVKEPQLPECARLKASQTLFAYLHLAADKP
                     QAEALMASGATCIAYETVTEAQGGLPLLRPMSEVAGRMSVHVGAAYLHKASGGRGVLL
                     GGVPGVAPARVVIIGGGVAGVNAAQMAAGLSADVTLLDIDAHKLIGIDRMFHGRVKTV
                     SSSRAALAEALREADLVIGAVLIPGAAAPRLVTRDMLKTMKPGAVMVDIAIDQGGCFE
                     TSHPTTHEDPVFIVDGVVHYCVANMPGAVARTSTLALNNATLPYVLKMADMGVSAALA
                     ADPHLMNGLNVAGGKIRCKAVAEALRLPFAA"
     gene            1796..2260
                     /locus_tag="VF402_02350"
     CDS             1796..2260
                     /locus_tag="VF402_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7799161.1"
                     /translation="MANGDKFSDLDSFDRELIKVLRSDARATSADLGKAVGLSPSAAH
                     RRVKILEQKGVIAGYRAVIAENVQGKQGTVFVHVTLQDQRRETFEKFEKAAIACSAIE
                     ECHLMSGEADYLLKIVLRESLSYEDVHRDVLSTMPGVSKLVSQFSIRTVKMS"
     gene            complement(2328..2528)
                     /locus_tag="VF402_02355"
     CDS             complement(2328..2528)
                     /locus_tag="VF402_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX7799162.1"
                     /translation="MTIEARVRELDHRHQSLKSIIAREERSPSVDSLYLSELKRKKLK
                     LKEEIERIKTLMRQDASEATLQ"
     gene            2685..3269
                     /locus_tag="VF402_02360"
     CDS             2685..3269
                     /locus_tag="VF402_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX7799163.1"
                     /translation="MNKAKANQRLIVAISGASGAIYGIRALRMLRAADIESHLVVSRA
                     AALSLDAETDMSLADVQALADVVYKPGDVGAAIASGSFRTLGMLVAPCSIKSLSEIAT
                     GVTSSLLSRAADVCLKERRRLVLMVRESPLHLGHIRSMAAVTEMGAIVAPPVPAFYTR
                     PQSLDDMVGQSVARALDLFGIEAPVTRWGEDIAP"
BASE COUNT          558 a         1026 c         1112 g          638 t
ORIGIN      
        1 cgtcagagcg ccttgcgcat cacgtagtcg gtaaattcgt acggccccat gcgcacgggc
       61 gtctcggctt cctgcgtgaa gccggagcgc aggtaatagg cgatggcggg cgcattgtcc
      121 ttattgacgt tgagcgccag ttcgcgcgcc gggaaatggc ccagcgccgt gtcgatcagc
      181 gccttgccga ggccgtggcc gcgaaaatcg tcgcgcacgt agagtttctt cagccacagg
      241 cgctcgccgt ccagccaggc cgaggcgtag ccgacgtcga gatcgccgac gcgcgcgacc
      301 cagaagacat ggccgccgcg catctccgtc tccagcgcct cggtggcgta cagcacggcc
      361 agcatggcgt cgacgcggtc cggcccgatg atgttgcgat aacatttcgg ccagatgatc
      421 agcgccagtt ggcgcacgat ctccagctcc tccggcccga tgcgtcttat gcgtggcgcg
      481 cgcgccatag cctgccccgt ttccttgcaa cctgtctttc tactatgttt tcggaaacgt
      541 cgattgtcca acgaaatctt acggataggg gcgggcggcg gctcaggcgg cgaagggcag
      601 gcgcagcgcc tcggcgaccg ccttgcagcg gatcttgccg cccgcgacgt tcaggccgtt
      661 catcaggtgc ggatcggcgg ccagggcggc ggacacgccc atatcggcca ttttcagcac
      721 atagggcaag gtggcgttgt tcagcgccag cgtcgaggtg cgcgccaccg cgcccggcat
      781 gttggcgacg cagtaatgca cgacgccgtc gacgatgaag accgggtctt cgtgcgtggt
      841 cgggtgcgag gtctcgaagc agccgccctg atcgatggcg atgtcgacca tgaccgcgcc
      901 gggcttcatg gtcttcagca tgtcgcgcgt gaccagcctg ggcgccgccg cgccggggat
      961 cagcacggcg ccgatcacca ggtcggcctc gcgcagcgcc tcggccagcg cggccctgga
     1021 cgacgagacg gtcttcaccc ggccgtggaa catccggtcg atcccgatca gcttgtgcgc
     1081 gtcgatgtcg agcagggtca cgtccgccga caggcccgcc gccatctgcg ccgcgttgac
     1141 gcccgccacg ccgccgccga tgatgacgac cctggccgga gccacgcccg gaacgccgcc
     1201 cagcagcacg ccgcgcccgc cggacgcctt gtgcagatag gcggcgccga catggacgga
     1261 catgcgtccg gcgacctcgg acatcggccg caggaggggc aggccgccct gtgcttcggt
     1321 caccgtctcg taggcgatgc aggtcgcgcc cgacgccatc agcgcctcgg cctgcggctt
     1381 gtcggcggcg aggtgcaggt aggcgaacag ggtctgcgag gctttgagcc gcgcgcattc
     1441 gggcagttgc ggctccttga ccttgacgat catctcgccc gcgtcgaaca ccgcttccgg
     1501 cgtcggcagg atggcggcgc ccgccgccct gtagtcgtcg tcggaaaagc cgacgcccgc
     1561 gccggcttgc gtttcgacga acacccgatg gccgtggtgg gtcagttcgg cgacggcgtg
     1621 cgggacgagg ccgacgcggt attcgtggtt cttgatttcc ctgggaacgc ttattttcat
     1681 ggcttcgctc cgttgagcag cgttgttttt gcttcgatta tgatccgaaa cggattgcaa
     1741 attctggaaa ttttgtgact tgatctggca atctgacgga tatattttta gtttcatggc
     1801 caatggcgat aaattttccg atctggactc cttcgaccgc gagctgatca aggtcttgcg
     1861 cagcgacgcg cgcgccacct cggcggacct gggcaaggcg gtcgggctgt cgccgtccgc
     1921 cgctcaccgc cgcgtcaaga ttctggagca gaagggcgtg atcgcgggct accgcgccgt
     1981 catcgccgag aacgtgcagg gcaagcaggg cacggtgttc gtccacgtca cgctgcagga
     2041 tcagcgccgc gagacgttcg agaagttcga gaaggccgcc atcgcctgct cggccattga
     2101 ggaatgccac ctgatgtcgg gcgaggccga ttatctgctc aagatcgtgc tgcgcgaaag
     2161 cctgtcctat gaggacgtgc atcgcgacgt tttgtcgacc atgcccggcg tgtcgaagct
     2221 ggtcagccag ttctcgatcc gcacggtgaa gatgagctga aaaaaatccc cggcgggacc
     2281 accggggatt ctttgtgacg tcaggttttt tgaagatcgc gacgccttca ctgcaaagtc
     2341 gcctcgctgg cgtcctgccg catgagggtc ttgatccttt cgatttcttc cttgagctta
     2401 agcttctttc gcttcagttc gctaaggtat agagagtcca cagagggaga gcgttcttca
     2461 cgggcaatga tcgatttgag ggattgatga cgatggtcga gttcacgaac tctcgcttcg
     2521 atggtcattg gcggcatcct ccagggttgc tccgccggac cccttgtccg gctccctcag
     2581 ggaacacccg caggacgcga atgtagaatc acaaaccgtt tcatccgatc acggaaacgc
     2641 gagaaattcg gttgatgaac ggttaaacaa aatcttgcgg caaaatgaac aaagcaaaag
     2701 caaatcagcg tcttattgtc gccataagcg gagcgtccgg ggcgatctac ggcattcgcg
     2761 ccctgcggat gctgcgcgcg gcggacatcg agtcgcacct cgtcgtttcg cgcgcggcgg
     2821 cgctgtcgct ggacgcggaa accgacatga gtctcgccga cgtgcaggcg ctggccgacg
     2881 tggtttacaa gcccggcgac gtgggggcgg cgatcgcttc gggttcgttc cgcacgctgg
     2941 ggatgctggt cgcgccgtgt tcgatcaaga gcctgagcga gatcgccacc ggcgtgacgt
     3001 ccagccttct gtcgcgcgcg gcggacgtgt gtctgaagga gcggcggcgg ctggtgctga
     3061 tggtgcgcga aagcccgctg catctgggcc atatccgttc gatggcggcg gtgaccgaga
     3121 tgggcgcgat cgtcgcgccg ccggttccgg cgttttacac ccggccgcaa agcctcgacg
     3181 acatggtggg gcaaagcgtg gcgcgggcgc tggacctgtt cgggatcgag gcgccggtga
     3241 caaggtgggg cgaggatatt gcgccctgat ttctggacct atgatttttg ggattgggcg
     3301 gtgcgcgcct atgcgggcga gggcgtggcc gagg
//
LOCUS       DASVLL010000143         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_384027,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000143 DASVLL010000000
VERSION     DASVLL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384027"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VF402_02365"
     CDS             complement(<1..867)
                     /locus_tag="VF402_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX7799164.1"
                     /translation="MLSFLRDRPEPLTVRAQTRLNGAGFAARLGGWFTRDHACALLSD
                     GGAYGRYSYLTALPDVMDAVAHDVEADVAGLLRAACAGGGEASSVFTGGVIGLAAFEL
                     GARLEGLRRRPFRIEGGAAWPELLLLRYPAVLVFDHAARSLTAYGRGPDAAAARAAMA
                     RLVALYEATPETPPHDGAFLDGPLLSETPDDAHEDKVAALVRQIHAGDLFQANLARGW
                     TGRLRRGLTPGDLAAALHARGPAPFGGYLRFGDRAVVSNSPERFVRLEADGAMETRPI
                     KDTRPRGATPAED"
     gene            complement(1055..2563)
                     /locus_tag="VF402_02370"
     CDS             complement(1055..2563)
                     /locus_tag="VF402_02370"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX7799165.1"
                     /translation="MNKPVKAEDAATLSASQAVSRLRDLYRQAVAHLQADLHAYLNDR
                     ALPSKAARGAGRYCYPKLTVIWRGQHDAEDEALIQHNRHPQVNRAFARFSRPGSYSSS
                     ITRPDIFGAHIEEQLELMIRDYGAEVSVGVSDQEIPYPYVLDGAGLDLNSVSALDLAR
                     HFPATELALIGNEVADGLDIKDAQGHHPLALFDGLRTDFSLARLKHYTGTPAEHVQSY
                     ILMTNYHRYVDVFVTYALEQLRKPDSPYTALSCCGNFVVTKDTPNPEELIANSPWRKH
                     QMPSYHLLAEGRAGISLVNIGVGPSNAKNITDHLAVMRPHVWLMIGHCGGLRPSQSIG
                     DYVLAHAYLRDDHVMDDVLPPEIPIPPIAEVQQALSQAAVAITGEDGDALKKRLRTGT
                     VVTTDDRNWELRYTLSALRFSQSRAVAIDMESATLAAQGYRFRVPYGTLLCVSDKPLH
                     GELKLPGQANHFYERAIREHIDIGIETMKILRGFGPKLHSRKLRAFDEPPFR"
     gene            complement(2623..>3882)
                     /locus_tag="VF402_02375"
     CDS             complement(2623..>3882)
                     /locus_tag="VF402_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7799166.1"
                     /translation="DHPLSSSIMPHLADKETLYLAMLLHDTGKGGEDGQEIGGEIAAR
                     KACERLGLEDWKVEQVAWLVRHHLILSDYAQKRDVSDPETVAAFARIVETPERLRQLL
                     ILTVADIRAVGPGVWNGWKGQLMRDLFAATESAFRGGRNSDALIGIRERLNEGASARR
                     VQLSENSADMAAWLETMDDTYLFAFTPEQVRQHAALVWSSFESEPAVKAHIDAQRNAT
                     AFSISADDRPGLFADLARAFANLGGNVVGAQVYTSRTGHALDVFYVQDAQGLPFAHDD
                     PGRLKQAEAQLEAAAQGRLPPPARWTSPLANRAAVFAIAPTVTFDDDANAAATIVEVS
                     GRDRPGLLADIVEVMAKAHLDIASAHIDCYGERAVDAFYVTDHFRKSQLSGGQKQALK
                     KALLAVMDQAPVAPKRNLARARASTAR"
BASE COUNT          604 a         1286 c         1357 g          635 t
ORIGIN      
        1 gtcctcggcg ggcgtggcgc ctcgcgggcg cgtgtccttg atcgggcgcg tctccatcgc
       61 gccgtccgct tcgaggcgca cgaagcgctc cgggctgttg gagacgacgg cgcgatcccc
      121 gaagcgcaga tagccgccga acggcgccgg gccgcgcgcg tgcagggcgg cggccagatc
      181 gccgggcgtc agcccccggc gcaaccgtcc ggtccagccg cgcgccagat tggcctgaaa
      241 caggtcgccg gcgtgaatct gccgcaccaa ggcggccacc ttgtcctcat gcgcgtcgtc
      301 gggcgtctcc gacagcagcg ggccgtcgag aaaggcgccg tcatggggcg gcgtttccgg
      361 cgtcgcctca tagagcgcga ccagccgcgc catcgcggcc cgcgccgccg ccgcatcggg
      421 gccgcgcccg taagcggtca ggctccgcgc cgcatgatcg aaaaccagaa ccgccggata
      481 gcgcagcagc aaaagctcag gccacgccgc gccgccctcg atgcggaacg gccgccgccg
      541 cagcccttcc agccgcgcgc ccagctcgaa cgccgccagc ccgatcacgc cgccggtaaa
      601 gacggacgag gcttcgccgc cgcccgcgca ggccgcgcgc agcagccccg ccacatcggc
      661 ctcgacatcg tgcgcgaccg cgtccatcac gtcgggcagg gcggtcagat agctgtaacg
      721 gccataagct ccgccatcgg acaatagcgc gcaggcgtga tcgcgcgtga accagccgcc
      781 cagccgcgcc gcaaatcccg ccccgttcag gcgcgtttgc gcccgcaccg tcaggggttc
      841 gggccgatca cgcaaaaagg ataacattca cagcaacttg attcgattgt ggcgccgccg
      901 tccggcatta tgatgaacag gatttgatat ttgagcgccg ccctgcggac tggatttcaa
      961 aacctatgga tggaggtcca ttgatcgact tttcaggagc ggatggcgga ccggccgggc
     1021 gtgccgcgct gtccactcct gatctttctc cccctcatcg gaacggcggt tcgtcgaagg
     1081 cgcgcaactt gcgcgaatgc agcttcgggc cgaagccgcg caggattttc atcgtctcga
     1141 tgccgatgtc gatatgttcg cggatcgcgc gctcatagaa atggttggcc tgtccgggca
     1201 gcttcagctc gccgtgcaaa ggcttgtccg acacgcacaa cagcgtgccg tagggcacgc
     1261 ggaagcggta gccctgcgcg gccagggtcg ccgactccat gtcgatggcc accgcgcgcg
     1321 actgcgagaa acgcagcgcg ctcagggtat agcgcagttc ccagttgcgg tcgtcggtgg
     1381 tgaccaccgt gccggtgcgc aggcgctttt tcagcgcgtc gccgtcctcg ccggtgatgg
     1441 cgacggcggc ctggctcagc gcctgctgca cctcggcgat cggcgggatc ggaatttccg
     1501 gcggcagcac gtcgtccatg acgtgatcgt cgcgcaggta ggcgtgggcc agcacgtaat
     1561 cgccgatgct ctgcgacggg cgcaggcccc cgcaatggcc gatcatcagc cagacatgcg
     1621 ggcgcatcac ggccagatgg tcggtgatgt tcttggcgtt ggacgggccg acgccgatat
     1681 tgaccagcga aatgcccgcc cgtccttcgg ccagcaggtg ataggacggc atctgatgct
     1741 tgcgccacgg tgaattggcg atcagttcct ccgggttcgg cgtatccttc gtcaccacga
     1801 aattgccgca gcacgacagc gccgtgtacg gactgtccgg cttgcgcaac tgctccagcg
     1861 cgtaggtgac gaacacgtcg acataacgat gatagttggt catcaggatg tagctttgca
     1921 cgtgttcggc gggcgtgccg gtatagtgct tcagccgcgc cagcgagaag tcggtgcgca
     1981 gcccgtcgaa cagggccagc gggtgatggc cctgcgcgtc cttgatgtcg aggccgtcgg
     2041 cgacctcgtt gccgatcagc gccagttcgg tggccggaaa gtgccgcgcc agatcgagcg
     2101 ccgacacgct gttgaggtcc agtcccgcgc cgtccagcac gtaggggtag gggatttcct
     2161 gatccgacac gccgaccgac acctcggcgc cgtagtcgcg gatcatcagt tcgagctgct
     2221 cctcgatatg ggcgccgaaa atgtccgggc gggtgatcga ggatgaatag gagccgggcc
     2281 gcgagaaacg cgcgaaggcg cggttgacct gcggatggcg gttgtgctgg atcagcgcct
     2341 cgtcttcggc gtcgtgctgg ccgcgccaga tcacggtgag cttcggatag caatagcggc
     2401 ccgcgccgcg cgccgccttc gacggcaggg cgcggtcgtt gagataggcg tgcaggtcgg
     2461 cctgcaaatg ggcgacggcc tgccggtaga ggtcgcgcag acggctgacg gcttgcgatg
     2521 cggacagcgt cgccgcgtcc tcggctttaa cgggcttgtt catgcgccct tagtatcatg
     2581 cgcgcgcgcg gcccgccaat cggcggcgtg tgaatttttc gcttaccgcg cggtcgaggc
     2641 gcgcgcgcgg gccaggttgc gcttgggcgc gacgggcgcc tgatccatca ccgccagcag
     2701 cgcctttttc aacgcctgct tctggccgcc ggacagttgc gacttgcgga aatggtcggt
     2761 gacgtagaag gcgtcgaccg cgcgctcgcc gtagcagtcg atgtgcgccg aggcgatgtc
     2821 gagatgcgcc ttggccatga cctcgacgat gtcggccaga aggccggggc ggtcgcggcc
     2881 cgacacctcg acgatggtgg cggcggcgtt ggcgtcgtcg tcgaaggtca cggtcggcgc
     2941 gatggcgaag acggcggcgc ggttggccag aggcgaggtc cagcgcgcgg gcggcggcag
     3001 gcgcccttgc gcggcggcct ccagctgcgc ctcggcctgt ttcaggcggc ccggatcgtc
     3061 gtgcgcgaac ggcaggcctt gcgcgtcctg cacgtagaag acgtcgagcg cgtggccggt
     3121 gcgcgaggtg tagacctgcg ccccgaccac gttgccgccc agattggcga aggcgcgcgc
     3181 cagatcggcg aacaggccgg gccggtcgtc ggccgagatc gagaaggcgg tggcgttgcg
     3241 ctgggcgtcg atatgcgcct tgaccgccgg ttcggattcg aagctggacc agaccagggc
     3301 ggcgtgctgg cgcacctgtt cgggcgtgaa ggcgaacaga taggtgtcgt ccatcgtctc
     3361 cagccacgcg gccatgtcgg cgctgttctc gctgagctgg acgcggcgcg cggaggcgcc
     3421 ttcgttcagg cgctcgcgaa tgccgatcag cgcgtcggaa ttgcgcccgc cgcggaaggc
     3481 gctctcggtg gcggcgaaca ggtcgcgcat caactggccc ttccagccgt tccacacgcc
     3541 cggcccgacg gcgcggatgt cggccacggt caggatcagc aactggcgca ggcgctccgg
     3601 cgtctcgacg atgcgcgcga aggcggcgac ggtttccggg tcggacacgt cgcgtttttg
     3661 cgcgtaatcg ctgaggatca ggtgatggcg caccagccac gcgacctgct cgaccttcca
     3721 gtcctccagc cccagccgtt cgcacgcctt acgcgcggcg atctcgccgc cgatctcctg
     3781 accgtcctcg ccgcccttgc cggtgtcgtg cagcagcatg gccagataaa gggtctcctt
     3841 gtcggccaga tgcggcatga tcgaggagga cagcgggtga tc
//
LOCUS       DASVLL010000144         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_330870,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000144 DASVLL010000000
VERSION     DASVLL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330870"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VF402_02380"
     CDS             complement(<1..574)
                     /locus_tag="VF402_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7799167.1"
                     /translation="MAVFKRLWASLALAAAVLAGAAAAHADPQFPDLNGQRVVDDAHI
                     LSDATKTDLTLKLQGLEQATTDQMVIVTVPDLQNYPIEDYGFQLGRHWGIGQSGQEKA
                     SNGQTYKDNGILLIVAPNERKVRIEVGYGLEPVMTDAMSGLIIRNAILPQFKAGDYDA
                     GVEAGTDAIIQQLSLDRGVAIKKAQAAQAQA"
     gene            complement(584..1198)
                     /locus_tag="VF402_02385"
     CDS             complement(584..1198)
                     /locus_tag="VF402_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7799168.1"
                     /translation="MKFTRVLGLIAAVGLIGALAGCGVNTIPTKQENARAKWSEVEVQ
                     YKRRADLIPQLVATVKGEAANERGILTDVINARASATKVSVDASTITDPEKFQKFQQA
                     QDGLSSAIGRLMVIQEQYPTLKSNQGFETLMDQLEGTANRIAIAQKDYNDAAQDYNTT
                     LRTFPSIIWAKTMYSGAKPMQYFTAPAGSDVAPTVDFSAAPASK"
     gene            1366..>2623
                     /locus_tag="VF402_02390"
     CDS             1366..>2623
                     /locus_tag="VF402_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799169.1"
                     /translation="MKRACAAALAAVLWAQPVWADPAAEKAATLPDLKAAAAAGGAEA
                     ENRLGLAYFNGEIVKQDFVQARQWFEKAAAQGYRAAEYNLGVIYDDGDGVAPDAAQAF
                     NWYKKAADQGDVSAAAQVGGMYMRGAGVGADTAAGMRYLLTAAAAGDATAETMLGYAY
                     HNGLGVKADAQAALEWYRLAAGDDDATAMYNLSRMYFDGEGVAEDDKTGFAWMKKAAE
                     RGDSDAQNMLGRCYREGWGVDRDDAQALIWYQKAADQDNAAAQDNLAWMYENGYGVAE
                     DDAKAYALYVKAAANGDDDAQERAALLLLQGIGVTKDADAALKILTERARKTAAAKAT
                     MGLVYLRGLGVAADYARALDWLTQAAEAGDAWAQYKLGDMYAGALGVKQDRRTALTWY
                     LIAAANGSDAARDAAAALRRQISHGDA"
BASE COUNT          425 a          793 c          975 g          430 t
ORIGIN      
        1 cggcctgggc ctgcgccgcc tgcgctttct tgatggcgac gccgcggtcc agcgacagtt
       61 gctggatgat ggcgtcggtt ccggcctcga ccccggcgtc gtaatcgccc gccttgaact
      121 gcggcaggat ggcgttgcgg atgatcaggc cggacatggc gtcggtcatg accggctcaa
      181 ggccgtagcc gacctcgatg cgcaccttgc gctcgttcgg cgcgacgatg agcaggatgc
      241 cgttgtcctt gtaggtctgg ccgttggacg ccttttcctg acccgactgg ccgatgcccc
      301 agtggcggcc gagctgaaag ccgtagtcct cgatcggata gttttgcagg tcgggcacgg
      361 tgacgatgac catctggtcc gtcgtcgcct gctccagccc ctgcaatttc agcgtcaggt
      421 cggtcttcgt cgcgtccgac aggatatgcg cgtcgtcgac gacccgctgc ccgttcaggt
      481 ccgggaattg gggatcggcg tgcgccgccg ccgcgcccgc aagcacggcg gcggccagcg
      541 cgaggctggc ccagaggcgt ttgaaaacgg ccatctcgac cgcttacttc gaggccgggg
      601 cggcggagaa gtcgaccgtc ggcgcgacgt ccgaaccggc gggcgcggtg aagtactgca
      661 tcggcttggc gccggaatac atggtcttgg cccagatgat gctggggaag gtgcgcaggg
      721 tggtgttata gtcctgcgcc gcgtcgttat agtccttctg cgcgatggcg atgcggttgg
      781 ccgtgccttc cagttggtcc atcagcgtct cgaagccctg attggacttc agcgtcggat
      841 actgctcctg aatcaccatc aggcggccga tggccgagga caggccgtcc tgcgcctgct
      901 ggaacttctg gaacttttcc gggtcggtga tggtcgaggc gtcgacgctg accttggtgg
      961 ccgaggcgcg cgcgttgatc acgtcggtca ggatgccgcg ctcgttggcg gcctcgccct
     1021 tcacggtggc gaccagttgc gggatcaggt cggcgcggcg cttgtactgc acctcgacct
     1081 cggaccattt ggccctggcg ttttcctgct tggtcgggat ggtgttgacg ccgcagcccg
     1141 cgagggcgcc gatcaggccg acggcggcga taagacccaa gacgcgcgtg aatttcatgt
     1201 gagcggctcc tgtgctcgaa aacgcaggga gcgtccgtat cgcgcccccc ttgcgtgtcg
     1261 tttatgtggg atgtcttagc gcaaattgaa gcccccgcca atctgggggg tggcgaaaga
     1321 ggcgcgatgc cgtcataaat gacgccggga cgaaggggga cggcgatgaa gcgggcatgt
     1381 gcggcggcgt tggcggcggt tctgtgggcg cagccggtat gggcggaccc ggcggcggaa
     1441 aaggccgcga ccctgcccga cctgaaagcc gcggcggcgg cgggcggcgc cgaggcggag
     1501 aaccggctcg gcctggccta tttcaacggc gagatcgtca agcaggactt cgttcaggcg
     1561 cggcaatggt tcgagaaggc cgccgcgcag ggctatcgcg ccgccgaata taatctcggc
     1621 gtgatctatg acgacggcga cggcgttgcg ccggacgcgg cgcaggcttt caactggtac
     1681 aaaaaggccg ccgatcaggg cgatgtctcc gccgccgcgc aggtcggcgg catgtatatg
     1741 cgcggcgccg gggtcggcgc cgatacggcg gcggggatga gatacctgct gacggcggcg
     1801 gcggcgggcg acgccacggc ggagaccatg ctgggctacg cctatcacaa cggcctcggc
     1861 gtgaaggccg acgcgcaggc ggcgctggaa tggtacaggc tggccgccgg cgacgacgat
     1921 gcgaccgcca tgtacaatct gagccggatg tatttcgacg gcgagggcgt ggcggaggac
     1981 gacaagaccg gcttcgcgtg gatgaaaaag gcggcggagc gcggcgattc cgacgcgcag
     2041 aacatgctgg gacgctgcta tcgcgagggc tggggcgtcg accgggacga cgcgcaggcg
     2101 ctgatctggt atcaaaaggc cgccgatcag gacaatgcgg cggcgcagga caatctggcg
     2161 tggatgtacg aaaacggcta cggcgtcgcc gaggacgacg cgaaggccta tgcgctttac
     2221 gtcaaggccg ccgccaatgg cgacgacgac gctcaggagc gggcggcgct gctgctgttg
     2281 cagggtatag gcgtgacgaa ggacgccgac gcggcgttga agatcctgac cgagcgcgcg
     2341 cgcaaaaccg ccgccgccaa ggcgacgatg gggctggttt acctgcgcgg actgggcgta
     2401 gccgccgatt acgccagggc gctggactgg ctgacgcagg cggccgaggc gggggatgcg
     2461 tgggcgcagt acaagctggg cgacatgtac gccggggcgc tgggcgtgaa gcaggaccgc
     2521 aggacggcgc tgacctggta cctgatcgcc gccgctaacg gcagcgacgc cgccagggac
     2581 gccgccgcgg ccctgcgccg gcagatcagc catggcgacg ccg
//
LOCUS       DASVLL010000145         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_277742,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000145 DASVLL010000000
VERSION     DASVLL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277742"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..992)
                     /locus_tag="VF402_02395"
     CDS             complement(156..992)
                     /locus_tag="VF402_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799170.1"
                     /translation="MRRFGPSFLISVLLHAALLALFFVSWVTVAPPITPPSVPVEIVS
                     EIPSREQAAAPVDQNAVLTPQPIPAPEEPVKPTPPPPEPAPKLPVPTPQKAVTPPAPK
                     PEKKPEPPKPAPAPPDKNGLKKPAPPKPSKARPSLDLDALAQLAAAPSRSPTRRQPQA
                     NTHATNGLSAVGSGPADAGEKQALSMLGDRLRHAWILNCDVPGSNKVNPEISFVLTPN
                     GRALQVNWVNRRSDPVWQAGASLAMAAVKKVAAESTDLPATIYGQKITFTFLGEKACE
                     DQ"
     gene            complement(996..1475)
                     /locus_tag="VF402_02400"
     CDS             complement(996..1475)
                     /locus_tag="VF402_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7799171.1"
                     /translation="MAMGVSGGAGSGRRGRRRSRRASLSEINVTPLVDVMLVLLIIFM
                     ISAPLLTSGVKIQLPKTEAAALKDEGDPLTVSIDEKGSLFVNDDPATYAQLTPRLLAI
                     TNGETDKPVYVRAAGSASYDIVAKVMAKLSTSGFVKINLLTEPMGHVAAASSSEAAQ"
     gene            complement(1488..2207)
                     /gene="tolQ"
                     /locus_tag="VF402_02405"
     CDS             complement(1488..2207)
                     /gene="tolQ"
                     /locus_tag="VF402_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX7799172.1"
                     /translation="MDASTASSASGGLNGALNFVHLFMHADIVVKAVMIVLLLASLWS
                     WAIIVQKIMALGKANKQADAFEGALSTGRALDDIAASLGTQPKEPMQKLLVNVTAAWG
                     EYKGRTISAAQGDLLIALVDRELNQTIAQESDTLEEGLSLLAVIATASPFIGLFGTVW
                     GIMNSFSAIASAGDTNLATVAPAISEALFATATGLFAAIPAYVGYNLFNARVARFSGR
                     METFADELMASLIRRLGGKLG"
     gene            complement(2312..2761)
                     /gene="ybgC"
                     /locus_tag="VF402_02410"
     CDS             complement(2312..2761)
                     /gene="ybgC"
                     /locus_tag="VF402_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX7799173.1"
                     /translation="MTQLSGQIIDHEHRLPIRVYYEDTDFSGVVYYANYLKFFERGRS
                     DYLRLIGIHHHELAALPEPLAFAVADIHVRYRAPARIDEVIETRTRLTALRGAGFTLR
                     QRILRGEQLLTEAEVQAVCIDLKGRPRRLPPALQAALSACAVNEKDA"
BASE COUNT          479 a          961 c         1011 g          509 t
ORIGIN      
        1 ggtcagaccc tgcgcgaagg cggcgggagc catcgccgca gcgaggccgc cggcgccgct
       61 taacagcgcg gcgcggcgcg tgaggttcat cttttcggga gtcgtcttga tcatggcgtt
      121 gctccttagt tgctggattt ccgcggcgga ggacatcact ggtcctcgca cgccttttca
      181 cccaggaagg taaaggtaat tttctgcccg tagatagtgg cgggcaaatc tgtcgattcc
      241 gcggcgacct ttttgaccgc cgccatagcc agactggcgc ccgcctgcca gaccggatcg
      301 ctgcggcgat tgacccagtt gacctgtagc gcgcggccat taggggtcaa tacgaaactg
      361 atctccggat tcaccttatt ggagccggga acgtcgcaat tgaggatcca ggcgtgtcgc
      421 aggcgatcgc ccagcatgct cagcgcctgc ttctcgcccg cgtccgccgg gccggagccg
      481 acggcggaca ggccgttggt ggcgtgggtg ttggcctgcg gctggcggcg ggtcggcgat
      541 ctggacggcg cggcggccag ttgcgccagc gcgtcgagat cgagcgacgg cctggccttg
      601 ctcggcttgg gcggggccgg ctttttcagg ccgttcttgt ccggcggcgc cggggcgggt
      661 ttcggcggtt ccggcttctt ctcaggcttg ggcgcgggcg gcgtcacggc cttttgcggc
      721 gtcggcacgg gcagtttggg cgcaggctcc ggcggcggcg gggtcggctt gaccggctcc
      781 tccggcgcgg gaatgggctg cggcgtcagc acggcgttct gatccaccgg cgccgccgcc
      841 tgttcgcggc tcgggatttc cgacacgatc tcgaccggca cggacggcgg cgtgatcggc
      901 ggcgcgaccg tgacccaact gacgaagaac agcgccagca gggccgcgtg cagcagcacg
      961 gagatcagga atgacggtcc gaacctgcgc atgtcctact gcgcggcctc cgacgacgac
     1021 gcggcggcga catgccccat gggctcggtc agcaggttga tcttgacgaa gccggaggtc
     1081 gacagcttgg ccatcacctt ggccacgatg tcatacgacg ccgaaccggc ggcgcgcaca
     1141 tagaccggct tgtccgtctc gccgttggtg atggccagaa ggcgcggcgt cagttgcgcg
     1201 taggtcgccg gatcgtcgtt gacgaacagg cttccctttt cgtcgatcga cacggtcagc
     1261 ggatcgccct cgtccttgag cgcggcggcc tcggtcttgg gcagttgaat cttcacgccc
     1321 gaggtcagca gcggcgccga gatcatgaag atgatcagca gcaccagcat gacgtcgacc
     1381 agcggcgtga cgttgatctc ggacaaagag gcgcggcggc tgcggcgacg tccgcgccgt
     1441 ccgcttcccg cgcccccgga aacacccatc gccatcgcag cgccccctta acccagcttg
     1501 ccgcccaggc ggcggatcag cgaggccatc agctcgtcgg cgaacgtctc catgcggccg
     1561 gagaagcgcg cgacgcgggc gttgaacagg ttatagccga cataggccgg aatggcggcg
     1621 aacaggccgg tggcggtggc gaacagcgcc tcggaaatag cgggcgcgac ggtggccagg
     1681 ttggtgtcgc ccgcgctggc gatggccgaa aacgagttca tgatgcccca gaccgtgccg
     1741 aacaggccga tgaagggcga ggcggtcgcg atcacggcca gcaggctcag gccctcctcc
     1801 agcgtgtccg attcctgcgc gatggtctgg ttcagttcgc ggtcgaccag cgcgatcaga
     1861 aggtcgccct gcgccgcgct gatcgtccgg cccttgtatt cgccccacgc cgccgtcacg
     1921 ttgaccagca gcttctgcat cggctccttc ggctgcgtgc ccagagaggc ggcgatgtcg
     1981 tcgagcgcgc ggcccgtgct gagcgcgccc tcgaaggcgt cggcctgctt gttggccttg
     2041 cccagcgcca tgatcttctg cacgatgatc gcccacgacc acagcgacgc cagcagcagc
     2101 acgatcatca ccgccttgac gacgatgtcg gcgtgcatga acaggtgcac gaaattcaga
     2161 gcgccgttca ggcccccgga agcggaactg gcggtgcttg catccatgcg catcccctcc
     2221 tcaaaatccg atatggtgaa tccttcaagt cgcgcacctt gcacgacatt tggcgacatt
     2281 tctatggcgc ggcagcatga aaccgcactt ttcaggcgtc tttttcgtta acggcgcagg
     2341 ccgagagcgc cgcctgcaag gcgggcggca gccgccgggg acgccccttc agatcgatgc
     2401 acaccgcctg cacctcggcc tcggtcaaaa gctgttcgcc gcgcaggatg cgctggcgca
     2461 acgtgaagcc ggcgccgcgc agcgcggtca ggcgggtgcg cgtctcgatc acctcgtcga
     2521 tgcgcgcggg cgcccggtag cgcacatgga tgtcggcgac cgcgaaggcc agcggctcgg
     2581 gcagggccgc cagttcgtga tggtgaatcc cgatcaggcg cagatagtcc gaacgcccgc
     2641 gctcgaagaa tttcaggtag ttggcgtaat agaccacgcc ggaaaaatcg gtgtcctcgt
     2701 aatagacccg gatcggcagg cggtgttcat ggtcgataat ctggccggaa agctgggtca
     2761 tgcgaacctt tcgataaggg cgatgctacc ccctctcccc tccatgaggg gagaggacga
     2821 ggagcaaggc gcagccgccg atcctcgggt gaggggccgc ccgccgactg agcgcaggcc
     2881 cctcacccga tcgccttcgc ttacgctcgg ctctcgtcct ctccccgcac gcggggagag
     2941 gggaaggttg cggttatagc
//
LOCUS       DASVLL010000146         4422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_384148,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000146 DASVLL010000000
VERSION     DASVLL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384148"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..760)
                     /gene="lipB"
                     /locus_tag="VF402_02415"
     CDS             complement(47..760)
                     /gene="lipB"
                     /locus_tag="VF402_02415"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7799174.1"
                     /translation="MVSETLNPDAAPPGFLRDDGEKPEWRVEAGLLAYPDAVALMEAR
                     AEAIAKGEARELVWLIEHPPLYTAGVSAKPEDLIAPDRFEVFASGRGGQYTYHGPGQR
                     VVYVMLDLNKRRKDVRAFVQALERWIIDTLWRFNVEGHIREGPVGVWVERRDKGYAPL
                     TEDKIAAIGVKLRRWVSFHGVSLNVEPDLSHFSGIVPCGITEHGVTSLLDLGLPVTMD
                     EVDDALKSSFETIFGPVER"
     gene            849..1106
                     /locus_tag="VF402_02420"
     CDS             849..1106
                     /locus_tag="VF402_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX7799175.1"
                     /translation="MSNSQVGAVPVEISVLLGRSVLPMQQLLRMGRGAVIPLDAHEHD
                     EVWILANNHPVARGEIQIFEEKISIVVTRPANVYDFMALGS"
     gene            1197..1281
                     /locus_tag="VF402_02425"
     tRNA            1197..1281
                     /locus_tag="VF402_02425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Leu,seq:gag)
     gene            1327..2793
                     /gene="mgtE"
                     /locus_tag="VF402_02430"
     CDS             1327..2793
                     /gene="mgtE"
                     /locus_tag="VF402_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX7799176.1"
                     /translation="MSQKTDASLDNDNLRDDETLTRGEDGPALSTPEAIEDFALGDDY
                     ALNPEYISLVIDAADRGDGMRLRELLDALRPADIADLLGFLSGDYREEVIPWIPSDAI
                     ADILPELDEDIREEVVETLHPQDLAEVLQELDSDDAAAVFEDLEEDQRLAVLAVMPEA
                     ERAAIGASMAYEEETAGRLMQREVMAAPSFWSVGQTIDRMRENGDGLPELFFDIYVLD
                     PSHKPLGAVPVSVLMRARRDMPLTQLMEPVTEIRVDQDQEEVAYIFEKYHLISAPVID
                     PSGRLVGQVTVDDIVNIIQEESQEDMLALAGVSDSGRDASIFGVVKSRFWWLLINLGT
                     AVLASSVIGLFQDAIAKLVALAVLNPICASMGGNAGTQTLTVAVRALATRELNAANLL
                     RTIWREIAAGVLNGLAFAAVMGVVTMLWFHDAKMALVIALAMIINLFAAAMAGILIPL
                     GLDRLGYDPAASATVFVTTVTDCVGFFSFLGLAVIILL"
     gene            2931..>4422
                     /locus_tag="VF402_02435"
     CDS             2931..>4422
                     /locus_tag="VF402_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEX7799177.1"
                     /translation="MRARLKVSRAGIELIKSFEGLRQTATRLPDGRWTLGYGHTFSAR
                     EGARVTQEDADALLRFDLLPIVDAVNNLVLVPLNQNQFDALVSFCFNIGVDNFAVSTV
                     LKRLNEGKPAEAAQAMDAWRSAEFNGQTYVLAPLIRRRAAEKNLFQTPETIDPSLGMT
                     VRPVEDVVETPAEAPLTAVRQVIDLNAPAAGDAAARQAEALAEARRAEDARLREQERL
                     ERERLEREAAARLEAERAEQQRLAAEREAALEAERREAARLEAERLAAEHLAREQAAR
                     EDALRQEAARREAERLEAEKAAQAAAEQSAADDAAEKARKAEAAAALMRLYSPYGGGA
                     LGRPLVAPRPVAPSQESFVQPTPIAPAATETAPIVISAQADAAPEPVVAEAEPEAAPP
                     VESPAESPVFQSASVMPPPVITTINPYARPQTPAPQPEAPEVRAAAPAVDVTQAPFVQ
                     ASPLNWREQLQRPLPQAEPDQAETPPPVTAARGASHFDEDYGDDDGW"
BASE COUNT          747 a         1406 c         1538 g          731 t
ORIGIN      
        1 cgggccgagt ctgtacaaac cggtgacgcc gcgcgaggcg taggttttag cgctcaaccg
       61 gtccgaagat cgtctcgaag ctggatttca gcgcgtcatc gacttcgtcc atcgtcaccg
      121 gcaggccgag gtcgagcagg ctggtgacgc cgtgttcggt gatgccgcac ggcacgatgc
      181 cgctgaaatg cgacaggtcg ggctcgacgt tcaggctgac gccgtggaag ctgacccagc
      241 ggcgcagctt gacgccgatg gcggcgatct tgtcttcggt caggggcgcg tagcccttgt
      301 cgcggcgctc gacccagacg ccgacggggc cctcgcggat atggccctcg acgttgaagc
      361 gccacagcgt atcgatgatc cagcgctcca gcgcctgcac gaaggcgcgc acgtccttgc
      421 ggcgtttgtt cagatccagc atgacataga cgacgcgctg gccggggccg tgataggtgt
      481 attgcccgcc gcgcccggag gcgaacacct cgaagcggtc cggcgcgatc aggtcttcgg
      541 gcttggccga cacgcccgcc gtatagagcg gcggatgctc gatcagccag acaagctcgc
      601 gcgcctcgcc tttcgcgatc gcctcggcgc gcgcctccat cagggcgacg gcgtcgggat
      661 aggcaaggag gcccgcctcg acgcgccatt cgggtttttc cccatcgtcg cgcagaaagc
      721 cgggcggggc ggcgtctgga tttaacgtct cggaaaccat gaggacggca tttatcataa
      781 agtcttaaag gatcagcccc taatatgggg cgtcccgaaa gatttggggc ttatccgggt
      841 aaaaacgcgt gtccaattcg caggtcgggg ctgttcccgt cgagatttcc gtgcttctgg
      901 gccgttcggt cctgccgatg cagcagcttt tgcgcatggg gcgcggcgcc gtgattccac
      961 tcgacgcgca cgagcatgac gaggtatgga ttttggccaa caaccacccg gtcgcgcgcg
     1021 gcgaaatcca gatcttcgag gagaagatct ccatcgtcgt cacgcgcccc gccaatgtgt
     1081 acgacttcat ggcgctcggt tcctgaagcc ctgtccacct gatttttcgg aatctgcgat
     1141 aaaaggcttc acaaagcgcc aaagtgttgc tatctgcgcg cctctccgga tgacgtgcgg
     1201 tcgtggcgga attggtagac gcgcagcgtt gaggtcgctg tggggcaacc cgtggaagtt
     1261 cgagtcttct cgaccgcacc atccgaagtt cagcgttata aacgccatag agaaggggcc
     1321 tgagcgatga gccagaagac cgacgcctct ctcgacaacg acaacctgcg tgacgacgag
     1381 accctgacgc gcggcgaaga cggcccggcg ctgagcacgc ccgaagccat cgaggatttc
     1441 gcgctcggcg acgattacgc gctcaacccg gaatatattt cgctggtgat cgacgccgcc
     1501 gaccgcggcg atgggatgcg cctgcgcgaa ctgctggacg cgctgcgccc ggcggacatc
     1561 gccgacctgc tgggcttcct gtccggggat taccgcgagg aggtcatccc gtggattccg
     1621 tccgacgcca tcgccgacat cctgcccgaa ctggatgagg acatccgcga ggaggtcgtc
     1681 gagaccctgc acccgcagga tctggccgag gtgttgcagg aactcgattc cgacgacgcc
     1741 gccgccgtgt tcgaagacct tgaggaggat cagcgcctcg ccgttctggc cgtcatgccg
     1801 gaggccgagc gcgccgccat cggcgcctcg atggcctatg aggaggagac ggccgggcgg
     1861 ctgatgcagc gcgaggtcat ggcggcgccc agcttctgga gcgtgggcca gaccatcgac
     1921 cggatgcgcg agaacggcga cggcctgccc gaactgttct tcgacatcta tgtgctggac
     1981 cccagccaca agccgctggg cgccgtgccg gtgtcggtgc tgatgcgcgc gcgccgcgac
     2041 atgccgctga cgcaactgat ggagccggtg accgagatcc gcgtcgatca ggatcaggag
     2101 gaggtcgcct atatcttcga gaaatatcac ctgatctcgg cgccggtgat cgatccgtcc
     2161 gggcggctgg tgggccaggt gacggtcgac gacatcgtca acatcattca ggaagaaagc
     2221 caggaggaca tgctggccct ggccggggtg tccgattcgg gccgcgacgc gtcgatcttc
     2281 ggcgtggtga agtcgcgctt ctggtggctt ttgatcaatc tcggcacggc ggtgctggcc
     2341 tcctcggtga tcggcctgtt tcaggacgcc atcgccaagc tggtggcgct ggccgtgctc
     2401 aatccgatct gcgcctcgat gggcggcaac gccgggacgc agaccctgac cgtggcggtg
     2461 cgggcgctgg ccacgcgcga actgaacgcg gccaacctgc tgcgcacgat ctggcgcgaa
     2521 atcgccgccg gggtgctgaa cgggctggcc ttcgcggcgg tgatgggcgt ggtgaccatg
     2581 ctgtggttcc acgatgcgaa gatggcgctg gtcatcgcgc tggccatgat catcaacctg
     2641 ttcgcggcgg cgatggcggg cattctgatc ccgctggggc tggaccggct gggctacgac
     2701 ccggcggcgt cggcgaccgt gttcgtgacc acggtgaccg attgcgtcgg ctttttcagc
     2761 ttcctggggc tggcggtcat aatcctgctg taagctccga aatatgcttg taaaatcggg
     2821 ctggtatatg gtttgcttgt tgacggcgcg tccgcccgaa gtgcgccatt tcgggacgga
     2881 tgggatggtc gcatccgcaa acgtttaggg cttggggtca ggattcgggg atgagagcgc
     2941 gtctgaaagt ctcccgcgcg ggaatcgagc ttatcaaaag cttcgagggg ctgcgccaga
     3001 cggcgacgcg cctgccggac gggcgctgga ccctgggcta tgggcacacc ttttccgccc
     3061 gcgaaggcgc gcgcgtcacg caggaggacg ccgacgcgct gctgcggttc gaccttctgc
     3121 ccattgtcga cgccgtcaac aatctggttc tggttccgct gaaccagaat cagttcgatg
     3181 cgctggtctc gttctgcttc aatatcggcg tcgataattt cgccgtctcg acggtgctga
     3241 agcgcctcaa cgagggcaag cccgccgagg cggcgcaggc gatggacgcg tggcgctcgg
     3301 ccgagttcaa cggccagacc tatgtgctgg ccccgctgat tcgccgccgc gcggcggaaa
     3361 agaacctgtt tcagacgccg gagacgatcg atccatcgct gggcatgacg gtgcgcccgg
     3421 tcgaggacgt cgtggaaacg ccggcggaag cgccgctgac cgccgtgcgg caggtgatcg
     3481 atctgaacgc gcccgccgcc ggggatgcgg cggcgcggca ggcggaggcg ctggccgaag
     3541 cgcgccgcgc cgaggacgcg cggttgcgcg agcaggaacg ccttgagcgc gaacggctgg
     3601 aacgcgaagc cgccgcgcgc ctggaggcgg agcgcgcgga acaacaacgg cttgcggccg
     3661 agcgggaagc ggctctggaa gccgaacgcc gggaggccgc gcgtctcgaa gcggaaaggc
     3721 tcgccgccga acatctggcg cgcgaacagg ccgcgcgcga ggacgccttg cgtcaggaag
     3781 cggcgcgccg tgaggccgaa cggctcgaag cggaaaaggc ggcgcaggcc gcggcggagc
     3841 agagcgccgc cgacgacgcg gcggagaagg cgcgcaaggc cgaggccgcc gccgcgctga
     3901 tgcggcttta ttcgccctat ggcggcggcg cgctgggccg tccgctggtc gcgcccagac
     3961 cggtcgcgcc gtcgcaggaa agcttcgtac agccgacgcc gatcgcgccc gccgcgacgg
     4021 aaaccgcgcc gatcgtgatt tcggcgcaag cggacgcggc gcccgaaccg gttgtcgccg
     4081 aagcggaacc ggaagccgcg ccgcctgtcg agtcgcccgc cgaatcgccc gtgtttcaga
     4141 gcgcgtcggt catgccgccg ccggtgatca ccacgatcaa tccctatgcg cgtccgcaaa
     4201 cgcccgcgcc gcagccggaa gcgcccgaag tccgcgccgc cgctccggcc gtcgacgtca
     4261 cgcaggcgcc gttcgtgcag gcttcgccgc tgaactggcg cgagcagttg cagcggccgc
     4321 tgccgcaggc cgagccggat caagcggaaa cgccgccgcc ggtgaccgcg gcgcgcggcg
     4381 ccagccattt cgatgaggat tacggcgacg acgacggctg ga
//
LOCUS       DASVLL010000147         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_492358,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000147 DASVLL010000000
VERSION     DASVLL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492358"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..998)
                     /locus_tag="VF402_02440"
     CDS             complement(228..998)
                     /locus_tag="VF402_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7799178.1"
                     /translation="MSHFNFYAVRALYTFEMSRMWRTLFQSLAAPVLSTSLYFIVFGS
                     AIGGRMTTVDGVAYGAFIVPGLTLLSILTESVSNASFGIYLPRFTGTIYELLSAPVSV
                     IEALLGYVGAAASKSLILGVVILVTARLFVDYQIAHPVWMIVFLLLNSLTFSLFGFIL
                     GVWADGFEKLQIVPLLVITPLTFLGGTFYSIHMLPALWQKIILFNPIVYLVNGFRWAF
                     YDKADVNMAISLGMTLGFMAICVAVIAWIFKTGYRLRT"
     gene            complement(995..1936)
                     /locus_tag="VF402_02445"
     CDS             complement(995..1936)
                     /locus_tag="VF402_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7799179.1"
                     /translation="MDPVIAITGLEKTYKGGFHALKGVDLSIRRGEIFALLGPNGAGK
                     TTLIGIVCGLVRPTGGTVIADGFDIAKDYRAARTRIGLVPQELATDMFETVQHTVAHS
                     RGLFGKPPNPALIEQILRDLSLWDKRNNKIATLSGGMKRRVLIAKALSHEPSILFLDE
                     PTAGVDVELRRDMWAMVRRLREAGVTIILTTHYIEEAEEMADRIGVINKGEIILVEDK
                     HALMKKLGQTRVQITLTQPLTAPPDIGREAQLSEDGLSLSFTLNGEGPDKTDDQGAGL
                     AEALKSLMAQGIDFSGVNAERSSLEDIFVNLVEDRAA"
BASE COUNT          408 a          642 c          687 g          405 t
ORIGIN      
        1 aaaaagatcg ccgtataatt gcgttaataa aattacaact attagttgtt attgtcgtcc
       61 tgtgttccgg aggcccctga aaccggaaca tcaacgcggg gcgcgccggt tttttctccc
      121 cttgtccggc gcgcattttg acccgccccg gaactgctgg tcacagttcc ggggcggagt
      181 ttttttgccg gctccgcgcc gcgaaggggg ctcccttttg gggcgtttca agtcctgagg
      241 cgatagccgg tcttgaatat ccacgcgatg accgccacgc agatcgccat gaagcccagg
      301 gtcatgccga ggctgatcgc catattgacg tcggccttgt cgtagaaggc ccagcgaaag
      361 ccgttgacca gatagacgat cgggttgaac aggatgatct tctgccacag ggcgggcagc
      421 atgtggatcg aatagaaggt gccgcccaag aaggtcagcg gcgtgatgac cagcagcggc
      481 acgatctgca acttctcgaa gccgtcggcc cagacgccga ggatgaagcc gaacaaagag
      541 aaggtcaggc tgttgagcag caggaagacg atcatccaca ccgggtgcgc gatctggtaa
      601 tcgacgaaca ggcgcgcggt gacgaggatc acgacgccga gaatcagcga cttcgacgcc
      661 gccgccccga catagccgag cagggcctcg atcaccgaca ccggcgcgga cagaagctcg
      721 tagatcgtgc cggtgaagcg cggcaggtag atgccgaacg aggcgttgga cacgctctcg
      781 gtcaggatgc tcagaagcgt caggcccggc acgatgaagg cgccgtaggc gacgccgtcc
      841 accgtcgtca tgcgcccgcc gatggccgag ccgaagacga tgaaatagag cgaggtcgac
      901 aaaaccggcg cggccagact ctggaacagg gtgcgccaca tccggctcat ctcgaaggtg
      961 tagagggcgc ggacggcgta aaagttaaaa tggctcatgc ggcgcggtcc tcgaccagat
     1021 tgacgaaaat gtcttcgagc gacgagcgct cggcgttgac gccggaaaaa tcgatcccct
     1081 gcgccatcag cgatttgagc gcctcggcga ggccggcacc ctgatcgtct gttttatctg
     1141 gcccttcgcc gttcagcgtg aaactcaagc tgaggccgtc ctcgctcaac tgcgcctcgc
     1201 ggccgatatc gggcggggcg gtcagcggct gggtcagggt gatctgcacg cgggtttggc
     1261 ccagcttctt catcagggcg tgcttgtcct cgacgaggat gatctcgccc ttgttgatca
     1321 cgccgatgcg gtcggccatt tcctcggcct cctcgatata gtgggtggtc aggatgatgg
     1381 tcacgcccgc ttcgcgcagg cggcgcacca tcgcccacat gtcacggcgc agttcgacgt
     1441 cgaccccggc ggtcggctcg tccaggaaca ggatgctggg ttcgtgcgac agcgccttgg
     1501 cgatcagaac gcgccgcttc atgccgccgc tgagcgtggc gatcttgttg ttgcgcttgt
     1561 cccagagaga caggtcgcgc aggatctgct cgatcagggc cggattgggc ggcttgccga
     1621 acaggccgcg cgaatgggcg accgtgtgct gcaccgtctc gaacatgtcg gtggccagct
     1681 cctgcggcac aaggccgatg cgcgtgcgcg cggcgcggta atccttggcg atgtcgaagc
     1741 cgtcggcgat caccgtcccg ccggtgggcc gcaccaggcc gcagacgatg ccgatcagcg
     1801 tggtcttgcc cgcgccgttg gggccgagca gggcgaagat ttcgccgcgc cttatgctga
     1861 gatcgacgcc tttcagcgcg tgaaatccgc ccttatatgt cttttcaagc cctgtgatgg
     1921 cgatgaccgg gtccatgctg cgtcctgtgc ggtaagctgt gccgctccca tataggcgcg
     1981 tcctgtcaaa atgcgacagg gtatagcgtg acggcgattc atgttctata tgtgttctta
     2041 tgagcagcga tcttttcggc catgccgaac ctcaggcgaa aaccttatcc gtcgacgacg
     2101 cgtccaaacc gctggccgac cgtatgcgcc cgcgcagcct gg
//
LOCUS       DASVLL010000148         5483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_223087,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000148 DASVLL010000000
VERSION     DASVLL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223087"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..433
                     /locus_tag="VF402_02450"
     CDS             11..433
                     /locus_tag="VF402_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX7799180.1"
                     /translation="MIDDLYSREILRRTTQLRHVGHLAAPDGQADRVSKLCGSSIHVE
                     VNMRDGVVADFAQQVEACALGQASAAILSQTVIGASTDEIRAGRDALRALLSGAEAEI
                     PARFADLRVLESVKDFPARHASTLLAWEATLAAVSQAT"
     gene            598..828
                     /gene="yidD"
                     /locus_tag="VF402_02455"
     CDS             598..828
                     /gene="yidD"
                     /locus_tag="VF402_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7799181.1"
                     /translation="MTSLYERSVRAGLRAYKLSLSPYIGQACRFRPTCSEYMAEALIV
                     HGPLKGGWMGVRRLCRCRPGGGHGYDPVPPRS"
     gene            954..2897
                     /gene="thrS"
                     /locus_tag="VF402_02460"
     CDS             954..2897
                     /gene="thrS"
                     /locus_tag="VF402_02460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7799182.1"
                     /translation="MTIDVTFPDGAIRSFEAGTTGRAIAESLSKSLAKKAVLIKLDGE
                     LRDLDRPLETGGKLEILTRDAPEVLEVIRHDTAHILAEAVQELFPGTQVTIGPSIEDG
                     FYYDFARDEPFSSDDFARIEAKMREIVDRDEKIVREVWDRDEAIRHFEAIGEAYKAEI
                     IRDLPAGETITVYRHGENWKDLCRGPHLPSSKFIGKAFRLTKLAGAYWRGDHNNAQLQ
                     RIYGTAWANEADLEAYITRIEEAEKRDHRRLGRQMDLFHMQEEGRGMVFWHPHGWTLW
                     RTLEAYMRRRLDAGGYVEVRTPQVLDRSFWEKSGHWDKYRANMFVCETVEGEVLSLKP
                     MNCPGHVQIFKVGLRSYKELPMRMAEFGACHRYEPSGSLHGLMRVRGFTQDDAHIFCR
                     EDQIVEETEKFIRLTRMIHKDLGMETHRINLATRPEIRAGSDDFWDKAEGMLLEAARA
                     AGVEPVIAEGDGAFYAPKLDFIVKDAIGREWTCGTLQLDYVLPERLEAEYVAEDGSRQ
                     RPVMLHRAILGSFERFIGIMIENFAGRFPMWLAPVQVVVAPITSDANPYAEKVAEAMR
                     AAGLRVEADLRNEKINYKIREHSLAKVPVIAVVGRKEAETGEVALRTLGAEGQNVMAL
                     AAAVAHLRAGATPPDLKPDLREG"
     gene            2902..3909
                     /locus_tag="VF402_02465"
     CDS             2902..3909
                     /locus_tag="VF402_02465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7799183.1"
                     /translation="MTHLPPSVVGLLEARKLETSEARYFMSHLDMRFDDAAEDGVTVV
                     MTVFHTGRVLFDAIDSVLREPLVRQFILIDNGSSEKTQGRLRELEASYEHVLLVQGQG
                     NIGFARAANLGAHLSNQPWLVFLNPDARLQPGCVQGLIEAARTGASPCVVGARVLNPD
                     LSEQRGARRGEITPVTTLLSLTRLSQIAPFLRRFEIHREADDLPLAPFPVPTISGACF
                     CVSKRDFARLKGFDASFFLHVEDVDLCWRAREMDGSVLFHPHAEVIHEGHTSRAEPVF
                     VEWNKGKGLIHYFHKRARGKGLWRKLYVWLLGPLILGVSLLRAASRPRLREDEDDFPA
                     E"
     gene            complement(3926..>5483)
                     /locus_tag="VF402_02470"
     CDS             complement(3926..>5483)
                     /locus_tag="VF402_02470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7799184.1"
                     /translation="MLETHDYIDINVQDVPRYKKPVGIHWLQVLAVKLTSKVERREVF
                     PYRWPSILGAALAAFACAWGASRAFGARVGAKAGLLFAVSFMLSTEAFIAKTDGVLCG
                     LTTLMMACLSQVYLRNRDLERDAVAPKMKAVKLVFWLALAGTILIKGPIGPMILATTL
                     LALWGWDRKIRWARSLGWGWGLILVLLLCGPWFVAITVKSDGQFWVGAVGHDLASKLD
                     GGSEGHFMWPGYHLGLLPITLFPAAWLLGGALQAAVTRRREAAVRFAVAWFLPAFVIF
                     ELSPTKLPHYPLPTFGALIWLCAIGLDAPRKVWANIINIAAGALGGLLIAAAAVIGYR
                     MYGQGALALTCVVLTAIGALGLAGFGGLVLLRRRAPEQTLSRAGFGALLAGGAVAHLA
                     LVGLLTQLHPLWVSKLMEQALVRNHLDPRSGITAGPVAVLGYAEPSFVFAMGTSTELD
                     NDDAKAAADALSDGRPVFVESRFEAPFQAAARAEGLTPHAVAVVKGHNYNGHDVSLTL
                     YDNPPDTAVQ"
BASE COUNT          944 a         1799 c         1862 g          878 t
ORIGIN      
        1 gtaagccggc atgatcgacg acctgtattc gcgtgaaatc ctgcgccgca cgacgcagtt
       61 gcggcatgtc ggccatctgg ccgcgccgga cggccaggcc gaccgcgtgt cgaaactgtg
      121 cggctccagc atccatgtcg aagttaacat gcgcgacggc gtggtggccg atttcgcgca
      181 gcaggtcgag gcgtgcgcgc tggggcaggc cagcgcggcg atcctgagcc agacggtgat
      241 cggcgcctcg acggacgaga ttcgcgccgg acgcgacgcc ctgcgcgcgc tgctgagcgg
      301 cgcggaggct gagattccgg cgcgtttcgc cgatctgcgc gtgctggaat cggtgaagga
      361 ttttcccgcc cgccacgcct cgaccctgct agcgtgggag gcgacgctgg ccgctgtttc
      421 gcaggcgacg taaatcgcgc ctcggcgccc gcgccaaatc gcgctgaaaa atccgcgctg
      481 attttatcac aatccccttg ccatagaggg taaaatgctg tcctatggtt gtgttccgcg
      541 ggggcgaaca cggcaggggg ttgacgcttt ttctcttttt cttcaatgag acgccgcatg
      601 acgtcgttgt acgagcgttc ggtgcgcgcc gggctgcggg cctataagct cagcctgtcg
      661 ccctatatcg gtcaggcctg ccgtttccgg ccgacctgct ccgaatatat ggccgaagcg
      721 cttatcgtcc acgggccgct gaagggcgga tggatgggcg tgcggcgtct gtgccgctgc
      781 cgtccgggcg gcggtcacgg ctacgatccg gtcccgccgc ggtcctgatc gcattccctt
      841 ccgtggggtc gttttcgcgt ttccggcgaa ggaccgcatg gaatctttat aaattttatt
      901 gcgcgatgaa gcccgccgta agggctacag gtcgccagac tgagagtaat gacatgacga
      961 tcgacgtaac cttccccgac ggcgcgattc gcagtttcga ggcgggaaca accggcaggg
     1021 ccatcgccga gagtctgtcg aaatccttgg cgaaaaaggc cgtgctgatc aagctggacg
     1081 gcgagctccg cgacctggac cggccgctgg agacgggggg gaagttggaa atcctgacgc
     1141 gcgacgcgcc ggaagttctc gaggtcattc gtcacgacac ggcccacatc ctggccgagg
     1201 cggtgcagga gctgtttccg ggcacgcagg tgacgatcgg cccgtcgatc gaggacggct
     1261 tctattacga cttcgcgcgc gacgaaccgt tctcgtcgga cgatttcgcc aggatcgagg
     1321 ccaagatgcg cgaaatcgtc gaccgcgacg aaaagatcgt gcgcgaggtc tgggaccgcg
     1381 acgaagcgat ccgccatttc gaggcgatcg gcgaagccta taaggccgag atcatccgcg
     1441 acctgcccgc gggcgagacg atcacggttt accgccacgg cgaaaactgg aaagacctgt
     1501 gccgcgggcc gcatctgccg tcgtcgaaat tcatcggcaa ggcgttccgg ctgaccaagc
     1561 tggccggcgc ctactggcgc ggcgaccaca acaatgcgca gttgcagcgc atctacggca
     1621 ccgcctgggc gaacgaggcc gatctcgaag cctacatcac ccgcatcgag gaggccgaga
     1681 agcgcgatca ccgccgtctg ggccgccaga tggacctgtt ccacatgcag gaagagggcc
     1741 ggggcatggt gttctggcat ccgcacggct ggacgctgtg gcggacgctg gaggcctata
     1801 tgcgccgccg tctcgacgcg ggcggctatg tcgaggtcag gacgccgcag gtgctggacc
     1861 gctcgttctg ggaaaagtcc ggccactggg ataagtaccg cgccaacatg ttcgtatgcg
     1921 agacggtcga gggcgaggtt ctgtcgctca agccgatgaa ctgccccggc catgtgcaga
     1981 ttttcaaggt cggtctgcgc tcgtacaagg agttgccgat gcgcatggcc gagttcggcg
     2041 cctgccatcg ctacgagccg tcggggtcgc tgcacgggct gatgcgcgtg cgcggcttca
     2101 cgcaggacga cgcgcacatc ttctgccgcg aggaccagat cgtcgaggag accgagaagt
     2161 tcatccgcct gacgcgcatg atccacaaag acctcggcat ggagacgcac cgcatcaacc
     2221 tggccacgcg cccggaaatc cgcgcaggca gcgacgactt ctgggacaag gccgagggca
     2281 tgttgctgga ggccgcgcgc gcggcgggcg tcgaaccggt gatcgccgag ggcgacggcg
     2341 ccttctatgc gcccaagctc gacttcatcg tcaaggacgc catcggccgc gaatggacct
     2401 gcggcacctt gcaactcgac tatgtgctgc cggagcggct ggaggcggaa tatgtcgccg
     2461 aggacggctc gcgtcagcgc ccggtcatgc tgcaccgcgc gattctcggc tcgttcgagc
     2521 gcttcatcgg catcatgatc gagaacttcg ccgggcgctt cccgatgtgg ctggcgccgg
     2581 tgcaggtggt ggtggcgccg atcacctcgg acgccaaccc ctacgccgaa aaggtcgccg
     2641 aggcgatgcg cgcggcgggc ctgcgcgtcg aggccgacct gcgcaacgaa aagatcaatt
     2701 acaagatccg cgagcactcg ctggccaagg ttccggtcat cgccgtggtc ggccgcaagg
     2761 aggccgaaac cggcgaggtc gccctgcgca cgctgggcgc cgagggccag aacgtgatgg
     2821 cgctggccgc cgccgtggcg cacttacgcg ccggggcgac gccgccggac ctgaagccgg
     2881 acttgaggga aggttgaacg catgacccac ctgccgccca gcgtcgtcgg tctgttggag
     2941 gcgcgcaagc tggaaaccag cgaagcgcgt tacttcatgt cgcatctcga catgcgcttc
     3001 gacgacgcgg ccgaggatgg ggtgacggtc gtcatgaccg tgttccacac cggcagggtg
     3061 ctgttcgacg ccatcgacag cgtgctgcgc gagccgctgg tccggcaatt catcctgatc
     3121 gacaacggct cgtccgaaaa gacgcaaggg cgcctgcgcg aactggaggc cagctacgaa
     3181 cacgtgctgc tggtgcaggg tcagggcaat atcggcttcg cgcgcgcggc caatctgggc
     3241 gcgcacctgt cgaaccagcc ttggctggtg ttcctgaacc cggacgcgcg tttgcagccg
     3301 ggctgcgtgc agggcctgat cgaggcggcg cgcaccggcg cctcgccctg cgtcgtcggg
     3361 gcgcgcgtgc tcaatcccga cctgagcgag cagcgcggcg cgcggcgcgg cgagatcacg
     3421 ccggtcacga cgctgctgtc gctgacgcgg ctgagccaga tcgcgccctt cctgcgccgc
     3481 ttcgagattc accgcgaggc cgacgacctg ccgctggcgc cgtttccggt gccgacgatt
     3541 tccggcgcct gtttttgcgt atccaagcgc gacttcgccc ggctcaaggg tttcgacgcc
     3601 agcttctttc tgcatgtcga ggatgtcgac ctgtgctggc gcgcgcgcga aatggacggc
     3661 tcggtgctat tccatccgca tgccgaggtc atccacgaag gccacacctc gcgcgccgaa
     3721 ccggtgttcg tggagtggaa caagggcaag gggctgattc actatttcca caagcgcgcc
     3781 aggggcaagg gcctgtggcg caagctctat gtctggctgc tggggccgct gatcctcggc
     3841 gtgtcgctgc tgcgcgccgc gtcgcgcccg cggctgcgcg aggacgagga cgactttccc
     3901 gctgaatagg cccgctgaat aagtcctact gcaccgccgt gtcgggcgga ttgtcgtaga
     3961 gggtcaggct gacgtcgtgg ccgttgtaat tgtggccctt gacgacggcg accgcgtgcg
     4021 gcgtcaggcc ctcggccctg gcggcggcct ggaacggcgc ctcgaagcgc gattcgacga
     4081 agaccgggcg cccgtcgctc agggcgtcgg cggcggcttt ggcgtcgtca ttgtccagtt
     4141 cggtgctggt ccccatcgcg aagacgaagc tcggttcggc atagccgagg acggccaccg
     4201 gaccggcggt gatgccgctg cgcggatcga gatggttgcg caccagcgcc tgctccatca
     4261 gcttcgacac ccacagggga tgcagttgcg tcagcagccc gaccagcgcc agatgcgcga
     4321 cggccccgcc cgccagcagc gcgccgaagc ccgcgcggct cagggtctgc tccggcgccc
     4381 ggcggcgcag caggaccagc ccgccgaaac cggcaagccc cagcgcgccg atcgccgtca
     4441 gcacgacgca ggtcaaggcc agcgcgccct gaccgtacat ccggtagccg atcacggcgg
     4501 cggcggcgat cagcagcccg cccagcgcgc ccgccgcgat gttgatgatg ttggcccaga
     4561 ccttacgcgg cgcgtcgagg ccgatggcgc acagccagat cagcgcgccg aaggtcggca
     4621 gcggatagtg cggcagcttg gtcggcgaca gctcgaagat gacgaaggcg ggcaggaacc
     4681 atgcgacggc gaagcgcacc gccgcctcgc gccgccgcgt gacggcggcc tgcaacgcgc
     4741 cgcccagcag ccacgccgcc gggaacaggg tgatcggcag caggccgaga tgatagcccg
     4801 gccacatgaa atggccttcg gagccgccgt ccagcttgct ggccagatca tggccgacgg
     4861 cgccgaccca gaactggccg tccgacttca ccgtgatcgc cacgaaccac ggtccgcaca
     4921 gcagcagcac caggatcagc ccccagcccc agccgaggct gcgcgcccag cggatcttgc
     4981 ggtcccagcc ccacagcgcc agcagggtcg tcgccaggat catcggcccg atcggcccct
     5041 tgatcaggat ggttccggcc agggccagcc agaagaccag cttgaccgcc ttcattttcg
     5101 gcgcgaccgc gtcgcgctca aggtcgcgat tgcgtaggta aacctgcgac aggcacgcca
     5161 tcatcagcgt ggtcaggccg cacagcacgc cgtcggtctt ggcgatgaag gcctcggtcg
     5221 acagcatgaa gctgaccgcg aacaacaggc ccgccttggc gccgacgcgg gcgccgaaag
     5281 cgcgcgacgc gccccatgcg caggcgaagg ccgccagcgc ggcgccgaga atcgacggcc
     5341 agcgataggg aaagacctcg cgcctttcga ccttcgaggt cagcttgacc gccagaacct
     5401 gcagccagtg gatgccgacc ggcttcttgt agcgcgggac gtcctgcacg ttgatgtcga
     5461 tatagtcgtg cgtctccagc atc
//
LOCUS       DASVLL010000149         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_384263,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000149 DASVLL010000000
VERSION     DASVLL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384263"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..424)
                     /locus_tag="VF402_02475"
     CDS             complement(107..424)
                     /locus_tag="VF402_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX7799185.1"
                     /translation="MDWTQILKAVFALAIVLGLILGLSAILRRYGPQFLAQMQAQRGR
                     KRLSVVETLVLDPARRLVLVRVDDEERLLLLGEGRELAAPGKTAAKSAKAGPASSPED
                     ELF"
     gene            594..1010
                     /gene="flgB"
                     /locus_tag="VF402_02480"
     CDS             594..1010
                     /gene="flgB"
                     /locus_tag="VF402_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX7799186.1"
                     /translation="MSDQGIAFLSALQNRMGWLSDRQKVVAENVANASTPGFKPSDLT
                     AQDFTAMVQGQAPDGGNLGLSMTDAMHIEPQGLGPAHARTVISPDSETTLNGNSVVLE
                     EQMLKMSESRMQFQAAVGFYEKSLAMIRMAAKAPGK"
     gene            1020..1436
                     /gene="flgC"
                     /locus_tag="VF402_02485"
     CDS             1020..1436
                     /gene="flgC"
                     /locus_tag="VF402_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX7799187.1"
                     /translation="MLKPLNSDQTMAVAASALKAQQARMRIIAENIANADSTGRTPGS
                     DPYRRQIPIFTTSPVDGATGVQLARVAQDTTDFTQEYDPSNPAADAKGYVKKPNVDPL
                     IETMDMREAQRAYEANMNVIDTARDMTSKILDILQK"
     gene            1448..1756
                     /locus_tag="VF402_02490"
     CDS             1448..1756
                     /locus_tag="VF402_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX7799188.1"
                     /translation="MTPLVAARAYGAALKQAQTLTGKDDADDAARPDFGKMVQTALAN
                     TVQSTQNAESLMVQQQAGKAELIDAVTAVANAETSLQSVIAIRDQVISAYQQIMSMPI
                     "
     gene            2011..2307
                     /locus_tag="VF402_02495"
     CDS             2011..2307
                     /locus_tag="VF402_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX7799189.1"
                     /translation="MIFELARLSVDPAKAADFEAAVARAAPFFQTAEGCHGMALERII
                     ETPGDYLLRVTWASVEHHMVAFRNSDNFQQWRALAGPFFLTPPQVTHSETISYF"
     gene            complement(2316..2450)
                     /locus_tag="VF402_02500"
     CDS             complement(2316..2450)
                     /locus_tag="VF402_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799190.1"
                     /translation="MALTLLTSATSKDSDAKDADTASSTHETYAASSAATGAAVDRVA
                     "
     gene            complement(2575..>2995)
                     /locus_tag="VF402_02505"
     CDS             complement(2575..>2995)
                     /locus_tag="VF402_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799191.1"
                     /translation="AGIAQDAANWDPNSHVATSGTVTVQQVAARYGLTDAISTDSDTV
                     SLSPAAQLAVANNTASAPAASDTSKSALQTLTGGAATVTAAGVALTLLTSATSKDSDA
                     KDADTAASTQKAYDPTGAAKDVAATSETSGAVVDLVA"
BASE COUNT          579 a          967 c          959 g          490 t
ORIGIN      
        1 tagggcagga tcatcaatgc gaaggcgagg acgaagacgc ccagcacgat gacgttcatc
       61 gccctggggc tgagcttgcg tttcggggcg ggcgcggcgt cggacatcag aacagctcgt
      121 cttcggggct tgaggcggga ccggccttgg cggatttggc ggcggttttg ccgggcgcgg
      181 ccagttcgcg gccttcgccc agcagcagca ggcgctcctc gtcgtcgacg cgcaccagca
      241 ccagccgccg cgctgggtcc agcaccagcg tctcgaccac cgacaggcgc tttctgccgc
      301 gctgcgcctg catctgggcc aggaattgcg ggccgtagcg gcgcaggatc gccgacaggc
      361 ccaggatcag gcccagcaca atcgccagcg cgaacaccgc tttgaggatc tgggtccagt
      421 ccattctcgc ccttgcttgt cacgtccgtt tttacggcgg catggttaac gcggcgttta
      481 cttaaaggct tcgttaacca aaaggtgggc agtatttgcc ctgttcccgg tgcgccgatt
      541 ccccgtttag agcgagtcgc gcgccgtagc gaaacggaaa ggcgagacga acgatgagcg
      601 atcagggcat tgcctttttg tcggcgctgc aaaaccgcat gggctggctg agcgaccgcc
      661 agaaggtggt ggcggagaac gtcgccaacg cctcgacgcc gggcttcaag cccagcgatc
      721 tgacggcgca ggatttcacc gccatggtgc agggccaggc gccggacggc ggcaatctgg
      781 gcctgagcat gaccgacgcc atgcatatcg agccgcaagg tctggggccg gcccacgcca
      841 gaaccgtcat cagccccgat tcggaaacca cgctcaacgg caacagcgtc gtgctggagg
      901 agcagatgct gaaaatgtcg gaaagccgga tgcagtttca ggcggcggtc ggcttctatg
      961 aaaagtcgct ggccatgatc cgcatggccg ccaaggcgcc gggcaagtag gagtaggcga
     1021 tgcttaaacc cctcaattcc gatcagacca tggccgtcgc cgccagcgcg ctcaaggcgc
     1081 agcaggcgcg gatgcgcatc atcgccgaga acatcgccaa cgccgattcg accggcagga
     1141 cgcccggctc cgatccgtac cgccggcaga ttccgatttt caccacctcg ccggtggacg
     1201 gcgcgaccgg cgtgcaactg gcccgcgtgg cgcaggacac cacggacttc acgcaggaat
     1261 acgacccgtc caaccccgcc gccgacgcca agggctatgt caagaagccg aacgtcgacc
     1321 cgctgatcga aaccatggac atgcgcgagg cgcagcgcgc ctacgaggcg aacatgaacg
     1381 tcatcgacac ggcgcgcgac atgaccagca aaattctcga catcttgcag aagtagggag
     1441 ctgaatcatg acgccactgg tcgccgcccg cgcctacggc gccgcgctga aacaggcgca
     1501 gaccctgacc ggcaaggacg acgccgatga cgcggccagg ccggacttcg gcaagatggt
     1561 gcagaccgcc ctggccaaca cggtgcaaag cacgcagaac gccgaaagcc tgatggtgca
     1621 gcagcaggcg ggcaaggccg agctgatcga cgcggtcacc gccgtcgcca acgccgagac
     1681 cagcctgcaa agcgtgatcg ccatccgcga tcaggtgatc agcgcctatc aacagatcat
     1741 gtccatgccg atctgaccca tcaaatcgaa aagcgctctt aaagaccgta aaacaggacc
     1801 gcgccgccat taggggccag gccgcgcgtg aaacgccgcg tagcatggcg ggaggatgaa
     1861 tggccgcgcg gccctgtcct tagaagcggg cgccgcccgc actggtgaac aaaccatata
     1921 ttgtaatcga ttacaagctg gcgccgcgtg acgaattcgc ctaaggcggc gggaccgcag
     1981 ccacaaaagc ccagcagcag gagtctggcc atgattttcg aactcgcccg cctgagcgtc
     2041 gatcccgcca aagccgccga tttcgaggcc gccgtcgccc gcgccgcgcc gtttttccag
     2101 accgccgagg gctgtcacgg catggcgctg gagcgcatca tcgaaacgcc gggcgattat
     2161 ctgctgcgcg tgacatgggc gagcgtcgag caccacatgg tcgccttccg caactccgac
     2221 aatttccagc aatggcgcgc cctggccgga ccgttcttcc tgaccccgcc gcaggtcacg
     2281 cactcggaaa cgatcagcta tttctgatcc cgccctcagg cgacccgatc gaccgccgcg
     2341 cccgtcgccg ccgaactggc ggcgtaggtc tcatgggtgg aggaagcggt atcggcgtcc
     2401 ttcgcatcgc tgtctttcga cgtggcgctt gtcaggagcg tcagagccac gcctgcgccg
     2461 gtgacggcat ccgcgccgcc ggtcagggtt tgcagcgcgg atttcgagga atcggtatcg
     2521 accgggtagg gccgttagac cgccttcctg atatatagaa aggcaaatct gtccttacgc
     2581 gaccagatcg acaacggcgc ccgaagtttc agaagtcgcc gccacgtctt tcgccgcgcc
     2641 ggtggggtcg taggccttct gggtggaggc ggcggtatcg gcgtccttcg catcgctgtc
     2701 tttcgacgtg gcgctcgtca ggagcgtcag agcgacgcct gcggcggtga cggtagccgc
     2761 gccgccggtc agcgtttgca atgccgattt tgacgtatcg gaagcggcgg gcgccgaagc
     2821 cgtgttgttg gcgacggcaa gttgcgccgc tggagacagg gaaaccgtgt cgctatccgt
     2881 cgatatggcg tcggtcaggc catatctggc cgcgacctgc tgtacagtca cggttcctga
     2941 agtcgcaacg tggctgttgg gatcccaatt cgcggcgtcc tgcgctattc ccgct
//
LOCUS       DASVLL010000150         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_9230, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000150 DASVLL010000000
VERSION     DASVLL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9230"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VF402_02510"
     CDS             complement(<1..489)
                     /locus_tag="VF402_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX7799192.1"
                     /translation="MIHNPVLRGFNPDPSILRVGADYYIATSTFEWYPGIQLHHSTDL
                     VNWRLLPRPLRRPSQLDLRGVPDSCGVWAPCLSHDGRRFYLTYSLVRRYGRTTRYGAE
                     GSSLRDFSNFVISCDSIEGEWSDPVFLNSSGFDPSLFHDRDGKTYLLNLLWDHRSGHD
                     RFG"
     gene            complement(486..1754)
                     /locus_tag="VF402_02515"
     CDS             complement(486..1754)
                     /locus_tag="VF402_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799193.1"
                     /translation="MPRFQKGGVRWGIIGLVTIGTIINYLTRSTLSVAAPTLMTSLHM
                     SEKNYGIITGVFQFGIMLQPVAGFLLDIVRLRLGLFLFALAWGLITMAHALAGSWPVL
                     AWLRGLQGFVEGVAQPGGMKVVAEWFPARERGLAGGLFNIGASLGGVLAPPLVAWAIL
                     YYNWRAAFLITGLLAVVWACLWLAAYRAPQRHPALGEAERAYIEAGQERHLASVAVRP
                     SPFRILRNRNFWGIAIPRFLADPTWSTLVFWLPLYLSQARGFDLRHIALFAWLPFVAA
                     DLGCLFGPFVVGLLQKAGAPLIKARRLTFTLGAVMMVGIVFVATVKSPYAAIALLCLA
                     AFAHQTLSVTVITMSSDLFRQSEVATVTGMAGTAANLGALLFSLAIGALVATTGYAPF
                     FYILGGVDLLGALVLWLLVRPGDAPAEGGA"
     gene            complement(2000..>2780)
                     /locus_tag="VF402_02520"
     CDS             complement(2000..>2780)
                     /locus_tag="VF402_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799194.1"
                     /translation="PEIAQNFEFGAVLANPHWAPGFNASIDYYNIKVAGAIIGGLSAA
                     QEVQYCYDGSVPAACSAFNLNGANPYVNVTTFNAGSIQTSGVDYEVSYQHPHPLGLSG
                     NLILRALATNVQDFTTTNSLPGTIPVQSAGVNTGSTPDWKALFVQTYTTRDYSVSVQE
                     RWFSNGVFGTQYVVCDAGDCPASTTNNPTINTNTMKGATYIDLSGTYNLGKHAVAYFK
                     IDNLMNVDPVPSPQTNTGLDVNPALYDTLGRFYHAGVRFRF"
BASE COUNT          528 a          872 c          941 g          439 t
ORIGIN      
        1 gccgaagcgg tcgtggccgc tgcgatggtc ccagagcagg ttgagcagat aggtcttgcc
       61 gtcccggtcg tgaaacagcg acggatcgaa gcccgaactg ttgagaaaga ccgggtccga
      121 ccattcgccc tcgatggagt cgcaactgat gacgaagttg gagaagtcgc gcagcgaact
      181 gccttcggcg ccgtagcgcg tggtgcgtcc gtatcgccgg accagcgaat aggtcaggta
      241 gaaccgcctg ccgtcatggc tgaggcaggg cgcccagacg ccgcaactgt ccggcacgcc
      301 gcgcaggtcc agttgcgacg gccgccgcag cggacgcggc agcaggcgcc agttgaccag
      361 atcggtcgag tgatgcaact ggatgccggg ataccactcg aaggtcgagg tcgcgatata
      421 gtagtccgcg ccgacgcgca ggatcgaggg gtccgggttg aagccgcgca gaacgggatt
      481 atggatcatg cgccgccctc cgccggagcg tcgccgggcc tgaccaaaag ccacagaacg
      541 agcgcgccca gaagatcgac cccgcccaga atgtagaaga aaggcgcata gccggtcgtc
      601 gcgaccagcg cgccgatggc cagcgaaaac aacagggcgc cgagattggc cgcggtcccc
      661 gccatgccgg tcacggtggc gacttcgctt tgccggaaca gatcggacga catggtgatg
      721 accgtgaccg aaagcgtctg gtgcgcgaag gccgcgaggc acagaagcgc gatggcggcg
      781 taggggcttt tgaccgtcgc gacgaagacg atgccgacca tcatcacggc gcccagcgtg
      841 aaggtcaggc gccgcgcctt gatcagcggc gcgcccgcct tttgcagcag gccgaccacg
      901 aaggggccga acaggcagcc gagatcggcg gcgacgaacg gcagccaggc gaacagcgcg
      961 atgtgcctga ggtcgaagcc gcgcgcctgg ctgagataaa gcggcagcca gaagaccagc
     1021 gtgctccagg tggggtcggc cagaaagcgc ggaatcgcga tgccccagaa gttccggttg
     1081 cgcaggatcc tgaacggcga cggacggacg gcgaccgagg cgagatggcg ttcctgcccc
     1141 gcttcgatat aggcgcgctc ggcctcgccc agcgccggat ggcgttgcgg cgcccggtag
     1201 gccgccagcc acagacaggc ccagaccacc gccagcaggc cggtgatcag aaacgcggcg
     1261 cgccagttgt agtaaaggat ggcccacgcc accagcggcg gcgccagaac gccgcccagc
     1321 gacgcgccga tgttgaacag gccgcccgcc agtccgcgct ccctggccgg aaaccactcc
     1381 gcgaccacct tcatgccgcc gggctgcgcc acgccttcga cgaagccctg caggccccgc
     1441 agccacgcca gcaccggcca gctgcccgcc agggcgtgcg ccatcgtgat cagcccccag
     1501 gccagggcga acaggaaaag gccgagccgc aggcgcacga tgtcgagcaa gaagccggcg
     1561 acgggttgca gcatgatgcc gaactgaaag acgccggtga tgatcccgta gttcttctcg
     1621 ctcatatgca ggctggtcat caaggtcgga gccgccacgc tgagcgtgct gcgcgtcaga
     1681 tagttgataa tcgtgccgat cgtgacgagg ccgataatgc cccacctcac gccgcccttt
     1741 tgaaatcggg gcatctggtt cctcgcctgt tttccgcggc ccgcgcgccg tctcctgcga
     1801 taggaaagcg cgacaagccg attatggaaa gcaaatatat gacggcccgg acttgcgtca
     1861 gcgcgccggg ccgtcggtct tggggggcct caggcgccta gagcgctttt cgatctgatg
     1921 ggatcagatc ggcgctctaa gctcttgttt tatcgctctt ccttatccaa aaagtggaac
     1981 cactttttgg ggaaacgctc tagaagcgga accgcacccc ggcgtggtag aagcggccca
     2041 gcgtgtcgta gagcgccggg ttgacgtcga ggccggtatt ggtctgcggc gaaggcacgg
     2101 gatcgacgtt catcaggttg tcgatcttga aataggccac cgcgtgcttg ccgagattat
     2161 acgtcccgga caggtcgata taggtcgccc ccttcatcgt attggtgttg atggtcgggt
     2221 tgttggtggt cgaggccggg cagtcccccg cgtcgcagac cacgtactgc gtgccgaaca
     2281 cgccgttgct gaaccagcgt tcctgcacgc tcacggagta gtcgcgcgtc gtgtaggtct
     2341 ggacgaacag ggccttccag tcgggcgtgg agccggtatt gaccccggcg gactggaccg
     2401 gaatcgtgcc gggcagcgag ttggtcgtgg tgaagtcctg cacgttggtc gccagcgccc
     2461 gcaggatcag attgccgctc aggcccagcg gatgcggatg ctggtagctg acctcgtagt
     2521 ccacgccgct ggtctggatg gagcccgcgt tgaacgtggt gacgttcaca tagggattgg
     2581 ccccgttcag gttgaaggcc gagcaggccg ccggcaccga gccgtcatag cagtactgca
     2641 cctcctgcgc cgcgctgagg ccgccgatga tcgcgcccgc caccttgatg ttgtagtagt
     2701 cgatcgaggc gttgaagccc ggcgcccagt gcggattggc cagcacggcc ccgaattcga
     2761 agttctgcgc gatttccggc
//
LOCUS       DASVLL010000151         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_492554,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000151 DASVLL010000000
VERSION     DASVLL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492554"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /gene="secG"
                     /locus_tag="VF402_02525"
     CDS             complement(<1..452)
                     /gene="secG"
                     /locus_tag="VF402_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7799195.1"
                     /translation="MLFGILLTVQIVICVAMGGLILIQQSEGGALGMGGGPSGFMSAR
                     GAGNLLTRLTGILAFLFFANCVALTAVGNMQNRVKSAVDQVDTSGLTLPVSGASASAA
                     ASSSASSAGPSLSNLPLGAPAPAASQAPAPGKTSSPLTALPPPVTLPQA"
     gene            complement(575..1318)
                     /gene="tpiA"
                     /locus_tag="VF402_02530"
     CDS             complement(575..1318)
                     /gene="tpiA"
                     /locus_tag="VF402_02530"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7799196.1"
                     /translation="MPQTHLIAGNWKMNGLKSALPVARSIAAQSRDHPNARVALFPPA
                     TLLPLMAEALADSDVILGGQDCHHADSGAYTGDVSAAMLKDAGARMVILGHSERRHGH
                     LEPCALVARKARAALRAGLEPIICIGETLDQRQSGLTQQVLERQLTDSLPDELRDAHF
                     HVSYEPVWAIGTGLAATDAQIEAAFTLIRAHLHRRFGPDFVGHLLYGGSVKPQNARHI
                     LALPGISGALVGGASLTADTFMPIVAGAD"
     gene            1546..3480
                     /locus_tag="VF402_02535"
     CDS             1546..3480
                     /locus_tag="VF402_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7799197.1"
                     /translation="MISLFRDFTKSWIFTALMALLIASFAVFGLRDVFGSAGLNSVVT
                     AGDRKVTSQQFKTQFDNYKTQQAQQNNGQSITNEDFVKSGLYLQMLDAVAGQTAFSAW
                     LDQLGVKATPKMVVQQLAQIPAFFNSVTGRFDKDTYAQALAMRQINQADFEKDLGDQL
                     AVQQYSQAALSGVRAPRVLAALVSAYELQSRDARIFTLTPNSVTPPAAPTDAQLQTFY
                     NDRKAQLSLPELRTASVIRFSAGDYEKSVPVTDADLKKLYDQELPTLKSAETRTFTEI
                     TAPDAASANAIAADLKAGKSPADAARAHKGNVIAVADKAQADIPDAKIAAAAFALQKG
                     QVSGAVQGDLGYAVIRMDDVKGGETPSFESQRAKLTDEYRKSKAADTVNDLVHKFQDA
                     HDAGDDFAASAAKLNLKINTLPPMTAQGKTGNPQVDYSPYTDLVKAIFDLPAAGASSD
                     VQRMADGEYFVVKLDEIKPAGPPPLSEIKAELTQYYMMEKMSEAVQAKADEAKARLDK
                     GEDFARVAASYNAPVQTLTGLDRAKVRQTNLPETIANQIFDGQAGDRFETATDNRGVV
                     YILGRIDAIHQADAGAANAMAANARLQMGQLMSRDIAGVTETAARDLVKTRTYPAAAA
                     QALGVTPPAAEKGAGKKAKS"
     gene            3486..>4052
                     /locus_tag="VF402_02540"
     CDS             3486..>4052
                     /locus_tag="VF402_02540"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261930.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX7799198.1"
                     /translation="MTLSISDQAGGASESAFRKAYEAGQPSLVCRKLIDDLETPVSAY
                     LKLGRKRPFSFLFESVEGGALSGRYSILTLSPDLVWRCFGARAEIARGADIAEGRYTP
                     EAEPSLQSLRALVDANRMAIPDGLPPMASGLFGVFGYDMIRLMEPLGAGNPDPLDLPD
                     AVLVRPTVVCVFDNVAHEILLASAVWPDK"
BASE COUNT          734 a         1385 c         1319 g          614 t
ORIGIN      
        1 gcctgcggca gggtcaccgg cggcggcagg gccgtcagcg ggctggacgt cttgcccgga
       61 gcgggcgcct gagacgcggc cggcgccggg gcgcccagcg gcaggttgct cagcgacgga
      121 ccggccgagg acgcgctcga cgacgcggcg gcggaggcgg aggcgccgct gaccggcagg
      181 gtcaggccgg aggtgtcgac ctgatcgacc gccgacttga cgcggttctg catgttgccg
      241 accgccgtca gcgcgacgca gttggcgaag aacaggaagg ccaggatgcc ggtcaggcgg
      301 gtcagcaggt taccggcccc gcgcgcggac atgaagcccg acgggccgcc gcccatgccg
      361 agcgcgccgc cttcggactg ctgaatgaga atcagcccgc ccatggcgac gcagatgacg
      421 atctgaacgg tgagcaggat accaaaaagc atatcttcgt cttctgtggc cttgaaaaag
      481 ttgcacgccc ttagcatccc ccgcggcgct tggccatagg gttgtcggag cctgtgtcac
      541 atttatgcgc gccgcgcgcg attgcaaggc ccgcttaatc cgcgcccgcg acgatcggca
      601 tgaaggtatc ggcggtcagc gaggcgccgc cgaccagggc gccgctgatt cccggcaggg
      661 ccaggatgtg gcgtgcgttc tgcggcttga ccgagccgcc gtacagaaga tgaccgacga
      721 aatcggggcc gaaacggcga tgcaggtgcg cgcggatcag cgtgaacgcc gcctcgatct
      781 gcgcgtccgt cgccgccagc cccgtgccga tggcccagac cggttcgtag gacacgtgaa
      841 aatgcgcgtc gcgcaattcg tccggcaggc tgtcggtcag ttgccgctcc agcacctgct
      901 gcgtcaggcc gctctggcgc tggtccagcg tctcgccgat gcagatgatc ggctccagcc
      961 ccgcgcgcag cgccgcgcgc gccttgcgcg ccaccagcgc gcacggttcc aggtggccgt
     1021 ggcggcgttc ggagtggccc agtatgacca tccgcgcgcc cgcgtccttc agcatggcgg
     1081 cggagacgtc gccggtatag gcgcccgaat cggcgtggtg gcaatcctgc ccgccgagga
     1141 tcacatcgct gtccgccagc gcctcggcca tcagcggcag gagcgtggcg ggcggaaaca
     1201 gggcgacgcg ggcattggga tggtcgcggc tctgcgcggc gatagaccga gcgaccggaa
     1261 gcgcggattt cagtccgttc atcttccagt tgcccgcgat caaatgagtc tgtggcattt
     1321 atttccggtc tttgcggctt ctttttgcga agcggagtct tctccgtctt cgcgcgcgac
     1381 catagcccgc cgccccaagc gcggccaagc ggaaaaagcc aagaaatgct tgaaagcggc
     1441 aaaatctctt tgtaactgcc ttatgggcgc cgtaaagaca cggcgatatg cggcgcgcac
     1501 atctcaatgg cgcgccaacc tttacaggga cctgacgcag cgaccatgat cagccttttc
     1561 cgggacttca ccaaatcttg gatcttcacc gccctgatgg cgttgctgat cgcctccttc
     1621 gcggttttcg gcctgcgcga cgtgttcggc tcggcgggcc tgaacagcgt cgtcacggcg
     1681 ggcgaccgca aggtcacgtc gcagcagttc aagacccagt tcgataatta caagacccag
     1741 caggcgcagc agaacaacgg ccagtcgatc accaacgagg atttcgtcaa gtcgggcctc
     1801 tatctgcaga tgctggacgc cgtcgcgggc cagaccgcct tctcggcctg gctggaccag
     1861 ttgggcgtca aggcgacgcc gaagatggtc gtgcagcaac tggcgcagat tccggccttc
     1921 ttcaactcgg tgaccgggcg cttcgataag gacacctacg ctcaggcgct ggcgatgcgc
     1981 cagatcaatc aggccgactt cgaaaaggat ctcggcgacc aactggccgt gcagcagtac
     2041 agccaggccg cgctcagcgg cgtgcgcgcg ccgcgcgtgc tggccgcgct ggtgtcggcc
     2101 tatgagctgc aatcgcgcga cgcccgcatc ttcaccctga cgccgaacag cgtcacgccg
     2161 cccgccgccc ctacggacgc ccagcttcag accttctaca acgaccgcaa ggcgcagttg
     2221 tcgctgccgg agctgcgcac cgcctcggtc atccgcttct cggcgggcga ctacgaaaag
     2281 tccgtccccg tcaccgacgc cgacctgaaa aagctgtacg atcaggaact gccgacgctg
     2341 aaaagcgccg agacgcgcac cttcaccgag atcaccgcgc cggacgccgc ctcggccaac
     2401 gccatcgccg ccgatctcaa ggcgggcaag tcgcccgccg acgccgccag ggcgcacaag
     2461 ggcaatgtca tcgccgtcgc cgacaaggcg caggccgaca ttcccgacgc caagatcgcc
     2521 gccgccgcct tcgcgctgca aaaggggcag gtttccggcg ccgtgcaggg cgatctcggc
     2581 tacgccgtga tccgcatgga cgacgtcaag ggcggcgaaa cgcccagctt cgagagccag
     2641 cgcgccaagc tgacggacga ataccgcaag agcaaggccg ccgacacggt caacgatctg
     2701 gtgcataaat tccaggacgc gcacgacgcg ggcgacgatt tcgccgccag cgccgccaag
     2761 ctgaacctca agatcaacac gctgccgccg atgaccgcgc agggcaagac cggcaatccg
     2821 caggtcgatt attcgcccta caccgatctg gtgaaggcga tcttcgacct gcccgccgcc
     2881 ggcgccagct cggacgtgca gcgcatggcg gacggcgaat atttcgtcgt caagctggat
     2941 gagatcaagc ccgccggtcc gccgccgctc agcgagatca aggccgagct gacgcagtac
     3001 tacatgatgg aaaaaatgtc cgaagccgtg caggccaagg ccgacgaagc caaggcgcgc
     3061 ctcgacaagg gcgaggattt cgccagggtc gcggccagct ataacgcgcc ggttcagacc
     3121 ctgaccggcc tcgaccgggc gaaggtgcgc cagacgaacc tgcccgaaac catcgccaac
     3181 cagattttcg acggccaggc gggcgaccgg ttcgaaaccg ccacggacaa ccgcggcgtc
     3241 gtctatatcc tcggccgcat cgatgcgatc catcaggccg acgccggcgc cgccaacgcg
     3301 atggccgcca acgcccgcct gcagatgggc cagttgatga gccgcgacat cgcgggcgtc
     3361 accgaaaccg ccgcgcgcga tctggtcaag acccgcacct atcccgccgc cgccgctcag
     3421 gcgctgggcg tcacgcctcc ggccgccgaa aaaggcgccg gcaagaaagc gaaatcgtga
     3481 gcgccatgac cctcagcatc agcgatcagg cgggcggcgc gtccgaaagc gcctttcgca
     3541 aggcctatga ggcgggtcag ccgagtctgg tctgccgcaa gctgatcgac gatctggaga
     3601 cgccggtttc ggcctatctg aaactggggc gcaagcgtcc gttcagcttc ctgttcgaat
     3661 cggtcgaggg cggcgccctg tcgggccgct attcgatcct gaccctgtcg cccgatctgg
     3721 tctggcgctg tttcggcgcg cgcgccgaaa tcgcgcgcgg cgccgacatc gccgagggcc
     3781 gttatacgcc cgaagccgag ccaagcctgc aaagcctgcg cgcgctggtc gacgccaacc
     3841 gcatggcgat tcccgacggc ctgccgccga tggcgtcggg cctgttcggc gtgttcggct
     3901 acgacatgat tcggctgatg gagccgctgg gcgcgggcaa tcccgatccg ctcgacctgc
     3961 ccgacgccgt gctggtgcgc cccaccgtgg tctgcgtgtt cgacaatgtc gcgcacgaaa
     4021 tcctgctggc cagcgcggtc tggccggaca ag
//
LOCUS       DASVLL010000152         3703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_277974,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000152 DASVLL010000000
VERSION     DASVLL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277974"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF402_02545"
     CDS             complement(<1..203)
                     /locus_tag="VF402_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799199.1"
                     /translation="MRRRQNRRQNRHRNSCLSFCLTGALILSGCHAKPPQDAAVTRLP
                     APPQPGQVALYAAPDGISVQSRSG"
     gene            complement(276..1694)
                     /locus_tag="VF402_02550"
     CDS             complement(276..1694)
                     /locus_tag="VF402_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799200.1"
                     /translation="MTLSDITRRLKTLVAAGGVAALLGLGALSPAAYAADQAQDFKID
                     PKVQAQAMTVAPGLIASAHIKCEPTAAYLQGETDLEKDGKKVKGSMYEIACKTGPGFL
                     ATAYSPTEIAQVFTCVMADNIKAKQPNAATCELPENKPSYQWLTPVVQPYLPNCVVDN
                     ARVMGSTPPDSKEPKIDRYEVGCQGAIGGIIDYPQLGSDAPVNFLSCLIGADKGFTCQ
                     YTTNDAILASLKPVAAKADADCQVNNVRFVGVTKESDGFFYEFGCANKPGFIVMTGND
                     NSFKQSVPCAQAMGLGGCKFTDAGAAAADAKTTYSQILKTAGYACTVQDYNIVGSQPQ
                     TKRDYVEFKCPEQPWGLIGFVPQPGSTASVHVKDCFIEQSTRADCTFVTEPQLKAQLD
                     KLIKVAEPGKNCDLSQVRYIGESADTENALITELACVNKRGYIAVVAPDRKSLEATPC
                     KLAKAHGLDQSCTIPGNGTYSD"
     gene            1914..2660
                     /locus_tag="VF402_02555"
     CDS             1914..2660
                     /locus_tag="VF402_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7799201.1"
                     /translation="MIARELNEGVMTLRLDRPAKKNALTEAMYDDLSEGLNAAAADAA
                     VRAVTIAAAGDDFCAGNDIADFARGLPRLHAAADIEDLPVFRFLKALTFFEKPLIAAV
                     QGQAVGVGVTMLLHCDLVAAADDARLSLPFLKLGLVPEAGSTLLLPRRIGHQRAFAWM
                     ALGETVTAGEALEWGLINRVVARAELEETASGMARRVAALPPNALRRTKGFLRDGEAL
                     WQVVRAEGEVFRDLLFSPEAQAAFAAFMNR"
     gene            2767..3312
                     /locus_tag="VF402_02560"
     CDS             2767..3312
                     /locus_tag="VF402_02560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7799202.1"
                     /translation="MALDALAMETPALDQPVLDYVIALEQPSQADAVLALTDRAFGPG
                     RFVKTAERLRENSTPLADLSYVALDGARVIASVRLWPVFVQAADETRAPIAFLGPIVV
                     DEACRGHRIGSRLTEAAVTAAFAKGLRAVLLVGSRAFFEPLGFARAEGLIMPGPVDPN
                     RLLIRYATDGDALSGQVVRVL"
     gene            3353..>3703
                     /locus_tag="VF402_02565"
     CDS             3353..>3703
                     /locus_tag="VF402_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX7799203.1"
                     /translation="MTKIPLSDDMAAPETRAVFAALEAAGGAACVRFVGGCVRNALMG
                     RAISDLDLAAQLTPDETEAALAAAGLRSVPTGKAFGTITAVVDGKPFEITSLREDVET
                     DGRRAVVRYTTDWAK"
BASE COUNT          541 a         1139 c         1340 g          683 t
ORIGIN      
        1 ccggagcggc tctgcacgct gatcccgtcc ggggcggcgt agagcgcgac ctgaccgggt
       61 tgcggcggcg cgggcaggcg cgtgaccgcc gcgtcctgcg gcggtttcgc atggcatccg
      121 ctcaggatca gcgcgcccgt caggcaaaaa ctcaggcagg aattccggtg acgattctgg
      181 cgacgattct ggcggcgacg catagaggat agctccgtaa aaaaacaccc gtggagtccg
      241 gctccacggg tgtctcaaaa catcatgtcc gaattttagt cggaataggt gccgttgccg
      301 gggatcgtgc aggactgatc caggccgtgc gccttggcca gcttgcatgg cgtggcttcg
      361 aggctcttgc ggtccggcgc gacgacggcg atgtagccgc gcttgttgac gcaggccagt
      421 tcggtgatca gcgcgttttc cgtgtcggcg gattcgccga tgtagcggac ctgcgacagg
      481 tcgcagttct tacccggctc ggcgaccttg atcagcttgt cgagctgggc cttgagctgc
      541 ggttcggtca cgaaggtgca gtcggcgcgg gtcgattgct cgatgaagca gtccttgacg
      601 tggaccgagg cggtgctgcc cggctgcggc acgaagccga tcaggcccca gggctgttcc
      661 gggcacttga actcgacata gtcgcgcttg gtctggggct ggctgccgac gatgttgtag
      721 tcctgaaccg tgcaggcgta gccggcggtc ttgaggatct ggctgtaggt cgtcttggcg
      781 tcggccgccg ccgcgcccgc gtcggtgaac ttgcagccgc cgaggcccat ggcctgggcg
      841 cacggcacgg actgcttgaa gctgttgtcg ttgccggtca tgacgatgaa gcccggcttg
      901 ttggcgcagc cgaattcata gaagaagccg tcggattcct tggtcacgcc gacgaagcgc
      961 acattgttga cctggcaatc ggcgtcggcc ttggcggcca ccggcttcag cgaggcgagg
     1021 atggcgtcgt tggtggtgta ctgacaggtg aagcccttgt cggcgccgat caggcagctc
     1081 aggaagttca ccggcgcgtc cgagccgagt tgcggatagt cgatgatgcc gccgatggcg
     1141 ccctggcacc cgacctcata gcggtcgatc ttcggctcct tggaatcggg cggcgtggag
     1201 cccatgacgc gcgcgttgtc gaccacgcaa ttgggcaggt acggctggac caccggggtc
     1261 agccactgat agctgggctt gttttccggc agttcgcagg tcgcggcgtt cggctgcttg
     1321 gccttgatgt tgtccgccat gacgcaggtg aagacctgcg cgatctcggt cggcgaatag
     1381 gcggtggcca ggaagccggg gccggtcttg cacgcgattt cgtacatcga gcccttgacc
     1441 ttcttgccgt ccttttccag atcggtctcg ccctgcagat aggcggcggt cggctcgcac
     1501 ttgatgtgcg ccgaagcgat caggcccggc gcgacggtca tggcctgcgc ctggaccttg
     1561 gggtcgatct tgaagtcctg cgcctgatcg gcggcgtagg cggcggggct gagggcgccc
     1621 agaccgagca gggcggcgac gccgcctgcg gcgaccagcg ttttcagccg gcgggtgatg
     1681 tcggatagtg tcatggcgag cttctccctg ctctggaagg gtgtcgatga ggcgcgacgg
     1741 ttggcccgtc gtagtatcac tcgtaaaaag cgttgttgcg gtttgccccg ccgccggtga
     1801 aatttcaata ggcttgtgca acttttatgg ccgggacaag gcgcggcgct tgtccgaggc
     1861 gcatctctgg tttacgttga cgtaaactat agggcgataa ccgggaggac ggcatgattg
     1921 cgcgcgagtt gaatgagggc gtcatgacgc tgcggctgga ccgtccggcc aagaagaacg
     1981 cgctgaccga ggcgatgtac gacgacctga gcgaagggct gaacgcggcg gcggcggacg
     2041 cggcggtgcg cgcggtgacg atcgcggcgg cgggcgacga tttctgcgcg ggcaacgaca
     2101 tcgccgattt cgcgcggggg ctgccgcgcc tgcacgccgc cgccgatatc gaggatctgc
     2161 cggtgttccg cttcctcaag gcgctgacct tttttgaaaa gccgctgatc gccgccgtgc
     2221 agggtcaggc ggtgggcgtc ggcgtgacga tgctgctgca ttgcgatctg gtcgcggcgg
     2281 cggacgacgc gcgcctcagc ctgccgttcc tgaagctggg cctcgtgccg gaagccggtt
     2341 cgaccctgct gctgccgcgg cgcatcggcc atcagcgcgc cttcgcgtgg atggcgctgg
     2401 gcgaaaccgt tactgccggc gaggcgctcg agtggggcct gatcaaccgc gtcgtggcgc
     2461 gcgcggagtt ggaagaaacg gcgtccggca tggcgcgccg cgtcgccgcc ctgccgccga
     2521 acgcgctgcg ccggaccaag ggcttcctgc gcgacggcga agcgctgtgg caggtcgtgc
     2581 gcgccgaggg cgaggtcttt cgcgaccttt tgttcagccc ggaggcgcag gccgccttcg
     2641 ccgccttcat gaaccggtga catatccggt tgcataatgt gcgggggcgc gggcagggtt
     2701 cggcattgac ctgcgggccg tttggtttta tgccgcgcgc ccagcttatc aacaggagcg
     2761 ccgatcatgg ccctggacgc tctggctatg gaaaccccgg ccttggatca accggtcctc
     2821 gactatgtca tcgccctgga acagccgtcg caggcggacg ccgttctggc gctgaccgac
     2881 cgcgccttcg ggccgggccg cttcgtcaag accgccgagc ggctgcgtga aaattccacg
     2941 ccgctggccg atctcagcta tgtcgcgctg gacggggcgc gcgtgatcgc ctcggtgcgc
     3001 ctgtggccgg tcttcgtgca ggcggcggac gagacgcgcg cgccgatcgc gtttctcggc
     3061 cccatcgtcg tcgacgaagc ctgccgcggc caccggatcg gcagccgcct gaccgaggcc
     3121 gccgtgacgg cggccttcgc caaggggctg cgcgccgtgc tgctggtcgg gtcgcgcgcc
     3181 tttttcgagc cgctcggctt tgcgcgcgcc gagggcctga tcatgccggg gccggtcgat
     3241 ccgaaccgcc tgctgatccg ttacgccacg gacggcgacg cgctgagcgg gcaggtcgtc
     3301 agggttctgt aagaagcttg gacctgtcgc gcgatccatg cgatctaggc gcatgaccaa
     3361 gattccctta tccgatgaca tggccgcgcc ggaaacgcgc gcggtgttcg cggcgctgga
     3421 ggcggcgggc ggcgcggcct gcgtgcgctt cgtcggcggc tgcgtgcgca acgcgctgat
     3481 ggggcgcgcg atttccgacc tcgacctggc tgcgcaactg acgccggacg agacggaggc
     3541 cgcgctggcg gcggcgggcc tgcgcagcgt ccccaccggc aaggcgttcg gcacaatcac
     3601 ggcggtggtg gacggaaagc cgtttgaaat cacttcctta cgcgaagatg ttgagacgga
     3661 cgggcgccgc gccgtggtgc gctacacgac ggactgggcg aag
//
LOCUS       DASVLL010000153         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_492709,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000153 DASVLL010000000
VERSION     DASVLL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492709"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..797)
                     /locus_tag="VF402_02570"
     CDS             complement(93..797)
                     /locus_tag="VF402_02570"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEX7799204.1"
                     /translation="MSGAAFAVNPAAEAVLRRVGREGGPLLLIDDVLADPDAAREVAV
                     AAPFGPPLSAWYPGVNAPVPDAYRQALLPALRASLARAFGTPADAQLAIMSFFALSTR
                     RAEELAPRQRIPHYDQPDPGMLALVHYLAPDQGGGTGFFRHDATGFETISPARRDAYR
                     AAADAELTAHGDALTGFAGPDTPGFTLTGAVEARYNRLIVYPACALHCALFDGARLSP
                     DPRTGRLTANSFIRAV"
     gene            complement(808..>1611)
                     /locus_tag="VF402_02575"
     CDS             complement(808..>1611)
                     /locus_tag="VF402_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7799205.1"
                     /translation="EAVYNPLPNHLHVPLTLQAARAGKHVLCEKPMALNAAEIDALEE
                     AARSVHIMEAFMVRHSLQWIKARELIRAGAVGTPRMIQSYFSYENLDPGNIRNKVEWG
                     GGGLLDIGCYCIVAGRYFFEAEPLRAVALVKRSETFGTDVTASGLLDFGGGRQLSFTV
                     STEAGRSQTINVFGNAARLNLPIPFNQPVTSPSRVVVDNGATLDGQAAVTHTLPVADQ
                     YQLMGEAFAAAVRGEAPLPYGLDDARRNMRVLDALFASERSGGWVDIVQ"
BASE COUNT          255 a          556 c          568 g          232 t
ORIGIN      
        1 gaaggcgcgt cgggccttgc gctgtcgggg atagtgcgtt gcggcccgat aaagaatcag
       61 tacgaaagcc cgcgccggcg cataaatgcg tcttataccg cccggatgaa gctgttcgcg
      121 gtcagccgtc ctgtgcgcgg atcggggctg agccgcgcgc cgtcgaacag ggcgcaatgc
      181 agcgcgcagg ccggatagac gatcagccgg ttatagcgcg cctcgaccgc cccggtcagg
      241 gtgaagcccg gcgtgtcggg accggcgaaa ccggtcagcg cgtcgccgtg cgccgtcagt
      301 tcggcgtcgg ccgccgcccg gtaggcgtcg cgccgcgcgg gcgaaatcgt ctcgaacccc
      361 gtggcgtcgt ggcggaaaaa gccggtgccg ccgccctgat ccggcgccag ataatgcacg
      421 agggccagca tgcccggatc gggctggtcg taatgcggaa tgcgctggcg cggcgccagc
      481 tcttcggcgc ggcgcgtcga cagggcgaag aagctcatga tcgccagttg cgcgtccgcg
      541 ggcgtgccga aggcgcgcgc cagcgacgcg cgcagggccg gcagcagcgc ctgacgatag
      601 gcgtcgggaa ccggcgcgtt gacgcccgga taccaggcgc tgagcggcgg gccgaacggc
      661 gcggcgaccg cgacctcacg cgcggcgtcc gggtcggcca gcacgtcgtc gatcagcagc
      721 agcggcccgc cctcgcgccc gacgcgccgc agaacggcct cggcggcggg attgacggcg
      781 aaggccgcgc cgctcatcgg accccgctca ttgaacgatg tcgacccagc cgccgctgcg
      841 ttcggacgcg aacagggcgt cgagcacccg catattgcgg cgcgcgtcgt cgaggccgta
      901 gggcagcggc gcctcgccgc gcacggcggc ggcgaaggct tcgcccatca actgatactg
      961 atccgccacc ggcagggtgt gcgtgaccgc cgcctgcccg tccagcgtcg cgccgttatc
     1021 gacgacgacg cggctggggc tggtcaccgg ctggttgaac gggatcggca ggttgagccg
     1081 cgccgcattg ccgaagacgt tgatcgtctg gctgcggccc gcctcggtcg agacggtgaa
     1141 ggacagttgc cgccccccgc cgaaatcgag caggcccgac gccgtcacgt ccgtgccgaa
     1201 ggtttccgaa cgcttgacca gcgccacggc gcgcaacggc tcggcctcga agaaataacg
     1261 cccggcgacg atgcagtagc agccgatgtc cagcagcccg ccgccgcccc attcgacctt
     1321 gttgcggata ttgccgggat cgagattttc gtaactgaaa tagctctgga tcatgcgcgg
     1381 cgtcccgacg gcgcccgcgc ggatcagctc gcgcgccttg atccattgca gcgagtgccg
     1441 caccatgaag gcttccatga tatgtacgga gcgcgccgcc tcttccagcg cgtcgatctc
     1501 cgccgcgttc agcgccatcg gcttttcgca cagcacatgc ttgcccgccc gcgccgcttg
     1561 cagggtcagc ggcacgtgca ggtgattggg cagcggatta tagaccgcct c
//
LOCUS       DASVLL010000154         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_492789,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000154 DASVLL010000000
VERSION     DASVLL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492789"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VF402_02580"
     CDS             complement(<1..1011)
                     /locus_tag="VF402_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX7799206.1"
                     /translation="MTLDALKAQAAADANAVIVDQFKADPDRLARMSLDVAGLHIDVS
                     KQGWTRAGFDAAIAAVENSGLAEARERMWTGKDINVSEGRAVLHVALRDTDAENLRLR
                     GPEIARDVKSARDAMRDYAGAIRSGEITGATGKRFKTLIHIGIGGSDLGPRLVFQALA
                     PLQPQIDVRFVANVDGSELALALAGLDPAETLIIAVSKTFTTQETLANFLAARDWLVR
                     GLGEAGARAHMAAVSAAPDKTGAYGVAPDRVFGFKDWVGGRYSLWSAVSLSVIVALGY
                     DVYERLLDGARAMDRHVLTAPLERNAAVWLAAAQLYNRIGLDRPSRAVIPYVHRLRRL
                     AAF"
     gene            complement(1051..2016)
                     /locus_tag="VF402_02585"
     CDS             complement(1051..2016)
                     /locus_tag="VF402_02585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7799207.1"
                     /translation="MSDLTLITLSYRSGAQIARCLEALCAQDADIIHADNGSDDIDMQ
                     ALAARFPRVRQVQHGGNLGFAAGMNKAAALAASEWIGFVNPDAYVAPDWAEAMRAAMR
                     RRPDVSIFTALQLNADEPRRMDGAGDCLTFFGFPYRAGLGHILPDDLREAEAFSPCGA
                     AFVIRRSLFERLGGFDERFFCYCEDADLGFRARLLGEACLFAPAARTLHVGSASTGVR
                     SDFALWHGYRNRLWLYLKSMPLPLLIATLPIHIGLTLIGAVKDTLKGRKALPWSALGA
                     AVAGIGPILAARKNIQRERRITSLRLAKSLTWNLSKIAHRQPDFR"
     gene            complement(2013..>2226)
                     /locus_tag="VF402_02590"
     CDS             complement(2013..>2226)
                     /locus_tag="VF402_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX7799208.1"
                     /translation="YALGNDFRNSKNEEIKAVLTEKLMESNPVLRGAAVWALRQGANT
                     SDFAEMKRRHAAAETDPDVRAEWEAA"
BASE COUNT          371 a          728 c          775 g          352 t
ORIGIN      
        1 gaaggcggcg aggcggcgca ggcgatgcac gtaagggatg accgcgcggc tggggcggtc
       61 gaggccgatg cggttgtaaa gctgcgcggc ggccagccag acggcggcgt tgcgctccag
      121 cggcgcggtc agcacgtgcc ggtccatcgc gcgcgcgccg tccagcaggc gctcatagac
      181 atcgtagccg agcgcgacga tcaccgacag gctgaccgcc gaccacagcg aatagcgccc
      241 gccgacccag tccttgaagc cgaacacgcg gtccggcgcg acgccgtagg cgccggtctt
      301 gtccggcgcg gcggagacgg cggccatgtg cgccctggcg cccgcctcgc cgaggcccct
      361 gaccagccag tcgcgcgcgg cgaggaagtt ggccagagtc tcctgcgtcg tgaaggtctt
      421 ggacacggca atgataagcg tctccgccgg atcgaggccc gccagcgcca gcgccagttc
      481 ggagccgtcg acattggcga cgaaacggac gtcgatctgc ggctggagcg gcgccagcgc
      541 ctgaaacacc aggcgcgggc cgaggtcgga gccgccgatg ccgatatgga tgagggtttt
      601 gaagcgcttg ccggtagcgc cggtgatctc gccggagcgg atcgcgcccg cataatcgcg
      661 catcgcgtcg cgcgccgact tgacgtcccg cgcgatctcc ggcccgcgca ggcgcaggtt
      721 ttccgcgtcc gtgtcgcgca gcgcgacgtg cagcacggcg cggccttcgg agacgttgat
      781 gtccttgccc gtccacatcc gctcgcgcgc ctcggcgagg ccggagtttt cgacggcggc
      841 gatggcggcg tcgaaaccgg cgcgggtcca gccttgcttg gagacgtcga tatgcaggcc
      901 cgccacgtcg aggctcatgc gcgccagacg gtcgggatcg gccttgaact gatcgacgat
      961 cacggcgtta gcgtcggcgg cggcctgggc tttgagggcg tcgagggtca tggcggtctc
     1021 ctggggtgcg cgttcagctt tttcgggcgg ttaccggaaa tcgggctggc gatgggcgat
     1081 tttcgagagg ttccatgtca agcttttggc cagccgtaaa gacgttatcc ggcgttcgcg
     1141 ctgaatattt ttacgcgccg ccagaattgg tccgatgccc gcgacggcgg cgccaagagc
     1201 gctccacggc aaggctttgc ggcctttgag cgtgtccttg accgccccga tcagggtaag
     1261 accaatatgg atcggcaagg tcgcgatcag cagcggcagc ggcatggatt tcaggtacag
     1321 ccacagacga ttgcgatagc cgtgccacag cgcgaagtcc gagcgcacgc cggtcgaggc
     1381 cgatccgaca tgcaaggtcc gcgcggcggg cgcgaacagg cacgcctcgc ccagcagccg
     1441 ggcgcggaaa ccgagatcgg cgtcctcgca atagcagaag aagcgttcat cgaagccgcc
     1501 cagacgctca aacaggctgc ggcggatgac gaaggcggcg ccgcagggcg agaacgcctc
     1561 cgcctcgcgc aggtcgtccg gcaaaatgtg gcccagcccg gcgcggtagg gaaagccgaa
     1621 aaaggtcagg cagtcgcccg ccccgtccat ccggcgcggc tcgtcggcgt tcagttgcag
     1681 ggcggtgaag atcgaaacgt ccgggcggcg gcgcatggcc gcgcgcatcg cctcggccca
     1741 gtccggcgcg acataggcgt cgggattgac gaagccgatc cattcgctgg ccgccagcgc
     1801 cgccgccttg ttcatgcccg ccgcgaagcc gagattgccg ccgtgctgaa cctgccgcac
     1861 gcgcgggaac cgggccgcca gcgcctgcat gtcgatatcg tccgagccgt tgtcggcgtg
     1921 aatgatatcg gcgtcctgcg cgcacagggc ttcgaggcag cgcgcgatct gcgccccgga
     1981 ccggtaggag agcgtgatca gcgtcaggtc gctcacgccg cctcccattc cgcgcgcaca
     2041 tcaggatcgg tttccgcggc ggcgtggcgg cgcttcattt ccgcaaaatc agaagtgttt
     2101 gcgccctgac gcaatgccca caccgccgcg ccgcgcagta caggattgga ttccatgagc
     2161 ttttccgtca ggacggcttt tatttcctca ttttttgaat ttcggaaatc attgcccagc
     2221 gcatag
//
LOCUS       DASVLL010000155         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_116488,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000155 DASVLL010000000
VERSION     DASVLL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116488"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VF402_02595"
     CDS             complement(<1..862)
                     /locus_tag="VF402_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX7799209.1"
                     /translation="MPSLLAQYRRLSAALGQRIRLPEGFARVFIALAVLSLGLLDLAR
                     LFQSVFSQAFTLGALLSLSDTVFSDIIHGAPRVIAGAFLVMMSLGLYLRSRLAWVISV
                     ATVALSVALSLVLKTLPFNGVSVYGVVVLILLLAGRRHFQKSSLAAASLFAATSLTSL
                     LIYAAVGSYILGDQFSPRIRDIGSAAYFVVVTMSTVGYGDVTPQTPEARLFVVSIILL
                     GVTVFATSLSALLVPMVTRRAQILNQLRGRFMKHSDHFIITSQSALARNTSEELRQRG
                     KHVVLIIDGED"
     gene            complement(909..>1555)
                     /locus_tag="VF402_02600"
     CDS             complement(909..>1555)
                     /locus_tag="VF402_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX7799210.1"
                     /translation="DLMPEVEIVCRMAMAALPDSRVDWARYIDDYDAIRDRIAEVYPD
                     IYAGFSEKIKNPQGFHLDVAPRRLVWHTPNGKANFLPLPGLDVNTPVEDPDMLRLATV
                     RSHDQFNTTIYSYNDRYRGVYNDRMVVFMNSDDLADRGVKAGERIALETISADGVKRR
                     VDGLTALDYPMPRGAVAGYYPELNPLLPLDYYDRISGTPAAKSIPVRVVMTAST"
BASE COUNT          304 a          478 c          534 g          239 t
ORIGIN      
        1 cgtcttcgcc gtcgatgatc aggacgacgt gcttgccgcg ctggcgcagc tcctccgacg
       61 tgttgcgcgc cagcgcgctc tggctggtga tgatgaaatg atcggagtgc ttcatgaagc
      121 ggcctcgcaa ctggttcagg atttgggcgc ggcgcgtgac catcggcacg agcagggcgc
      181 tgagcgacgt ggcgaagacc gtgacgccga gcagaatgat cgaaacgacg aacaggcgcg
      241 cttccggcgt ctggggcgtg acgtcgccgt agccgaccgt cgacatggtg accaccacga
      301 aataggcggc ggaaccgatg tcccttatgc ggggcgagaa ctggtccccc agaatatagg
      361 agccgacggc cgcgtagatg agcagcgagg tcaggctggt cgccgcgaac agcgaggcgg
      421 cggccaggct gctcttctgg aaatggcggc gtccggccag cagcaggatc aggacgacga
      481 cgccgtaaac ggagacgccg ttgaacggca gggttttgag gacaaggctg agcgcgaccg
      541 acagcgcgac cgtcgccacc gagatgaccc aggccagccg cgagcgcaga tagagcccca
      601 gcgacatcat gaccagaaag gcgcccgcga tgacgcgcgg cgcgccgtgg atgatgtcgc
      661 tgaaaaccgt gtccgacagg gacagcagcg cgcccagcgt aaacgcctgc gaaaagacgc
      721 tctggaacag ccgggcgagg tcgagcaggc ccagactcag caccgccagc gcgatgaaga
      781 cccgcgcgaa tccttcgggc agccggatgc gctggccgag cgcggcgctt aatctgcggt
      841 actgagccag tagggatggc aagaaatgac ctcaaaatcg atggcgccga agcctcggcc
      901 gcatgccgtc aggtcgacgc cgtcatgacg acgcgcacgg gaatcgactt ggcggcgggc
      961 gtgccgctga tccggtcgta atagtcgagc ggcagcagcg gattgagttc agggtaatac
     1021 ccggccacgg cgccgcgcgg catcggatag tcgagcgccg tcagcccgtc gacgcgccgc
     1081 ttcacgccgt cggcgctgat ggtttccagc gcgatgcgct caccggcttt cacgccccgg
     1141 tccgccagat cgtcgctgtt catgaacacc accatgcggt cgttatagac gccgcgatag
     1201 cggtcgttat agctgtagat ggtggtgttg aactggtcgt gcgagcgcac cgtggccagc
     1261 cgcagcatgt cgggatcctc aaccggcgta ttgacgtcca gacccggcag cggcaggaaa
     1321 ttggccttgc cgttcggcgt atgccagacc agacgccggg gcgccacgtc gagatggaag
     1381 ccctgcgggt ttttgatctt ctccgaaaaa ccggcataga tgtcgggata gacctcggcg
     1441 atccggtcgc ggatggcgtc gtaatcgtcg atatagcgcg cccagtcgac gcggctgtcc
     1501 ggcagggccg ccatcgccat gcggcagacg atctcgacct ccggcatcag gtccg
//
LOCUS       DASVLL010000156         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_62405, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000156 DASVLL010000000
VERSION     DASVLL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62405"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VF402_02605"
     CDS             <1..1054
                     /locus_tag="VF402_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7799211.1"
                     /translation="DCRQDGAAIGPVAAGAARESWLFNSGIAGIEEADAILLVGTDLR
                     HEASLINARIRKVWLQNKTQVGVIGEAVDLTYDYAHVGLGGKALKDLRKHAFMDVLKN
                     ARKPAILVGMGAISGEDGAAVLKEIGALAASLNVVRDDWNGFNIVHTAAGRVAGLDLG
                     FTPVGEALDARAMLSGGVDLLFLLGADEFDASQLGATSVVYIGSHGDKGASVADIVLP
                     GAAYTEKSGIYTNLEGRVQISERAVFPKGEAKEDWAILRALSAHLGATLPYDSLTQLR
                     NKLITDHPVFGRIDVKPAAKAFDVKAIGGKGTVQDRVFASPIADFYLTNPIARASEAM
                     AECSAARRPQISAAAE"
     gene            1065..>1684
                     /locus_tag="VF402_02610"
     CDS             1065..>1684
                     /locus_tag="VF402_02610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX7799212.1"
                     /translation="MNSFWSSPWGWTIITVGETLAVIVGVMVSLAFLLWGDRKVWAGV
                     QMRKGPNVVGPFGLMQSFADFFKFVLKEVIIPAGSDKFIFLLAPLISFVLAFAAWAVI
                     PFAPGWVVSDINVGILYLLGISSLGVYGIIMGGWASNSKYPFLGGLRSAAQMVSYEVS
                     IGFIIVTVILLTGSINLSDIVRHQAGGIWNWNVFGGGHWPLLIVMIP"
BASE COUNT          269 a          522 c          576 g          317 t
ORIGIN      
        1 cgattgccgt caggacgggg ccgccatcgg gcctgtggcg gcgggcgcgg cgcgcgaatc
       61 gtggctgttc aattccggca tcgccgggat cgaggaggcc gacgcgatcc tgctggtcgg
      121 cacggatctg cgtcatgagg cgtcgctgat caatgcgcgc atccgcaagg tctggctgca
      181 aaacaagact caggtcggcg tgatcggcga ggccgtcgat ctgacctacg attacgcgca
      241 tgtcggcctc ggcggcaagg cgctgaaaga cctcaggaag cacgccttca tggacgtgct
      301 gaaaaacgcc aggaagcccg ccattctggt cggcatgggc gcgatctcgg gcgaggacgg
      361 cgccgccgtg ctcaaggaga tcggcgcgct ggccgccagc ctgaacgtcg tgcgcgacga
      421 ctggaacggt tttaatatcg tgcatacggc ggcgggccgc gtggccgggc tggacctcgg
      481 cttcacgccg gtcggcgagg cgctggacgc gcgcgccatg ctgagcggcg gggtcgatct
      541 tctgttcctg ctgggcgcgg acgaattcga cgcctcgcaa ctgggcgcga ccagcgtggt
      601 ctatatcggc tcgcacggcg acaagggcgc ttcggtggcc gacatcgtgc tgccgggcgc
      661 ggcctatacg gaaaagtcgg gcatctacac caacctcgaa ggccgggtgc agatttccga
      721 gcgcgcggtg ttcccgaagg gcgaggccaa ggaagactgg gccatcctgc gcgccctgtc
      781 ggcgcatctg ggcgcgaccc tgccgtacga cagcctgacg cagttgcgca acaaactgat
      841 caccgatcat ccggtgttcg gccgcatcga cgtcaagccg gcggccaaag ctttcgatgt
      901 caaggcgatc ggcgggaagg ggacggttca ggaccgcgtc ttcgcgtcgc ccatcgctga
      961 tttttatctg acgaacccga tcgcccgcgc ctccgaggct atggcggaat gctcggcggc
     1021 ccgcaggccg cagatcagcg cggcggcgga atagaggtaa cgagatgaat agtttctggt
     1081 catcgccctg gggctggacg atcataacgg tcggcgaaac gctggccgtg atcgtgggcg
     1141 tcatggtgtc gctcgccttc cttctgtggg gcgaccgcaa ggtctgggcc ggcgtgcaga
     1201 tgcgcaaggg ccccaacgtg gtcggcccgt tcggcctgat gcagtccttc gccgacttct
     1261 tcaagttcgt gctgaaggaa gtgatcattc ccgccggttc ggacaagttc atcttcctgc
     1321 tggcgccgct gatcagcttc gtgctggcct tcgccgcgtg ggcggtgatc ccgttcgcgc
     1381 cgggctgggt cgtgtcggac atcaatgtcg gcatcctcta cctgctcggc atttcgtcgc
     1441 tcggcgtcta tggcatcatc atgggcggtt gggcgtcgaa ctcgaaatat ccgttcctcg
     1501 gcggcctgcg ctcggcggcg cagatggtgt cctacgaagt ctcgatcggc ttcatcatcg
     1561 tcaccgtcat cctgctgacc ggctcgatca acctgtccga catcgtgcgc catcaggcgg
     1621 gcggcatctg gaactggaac gtgttcggcg gcgggcactg gccgctgctg atcgtcatga
     1681 tccc
//
LOCUS       DASVLL010000157         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_223650,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000157 DASVLL010000000
VERSION     DASVLL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223650"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VF402_02615"
     CDS             complement(<1..928)
                     /locus_tag="VF402_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX7799213.1"
                     /translation="MSSIKTFRRAALSLSPLLLAAAQAPVQAHAQAIERHLPETPQVT
                     TRALVTPNAVPNDQDAAPIGPALSAIVLLGPVEAAHDGAAPGVTVGEVDRLRADKARA
                     DIAARLRPFLGQTLSRKLIAQIEAAVAQDYRDLNYPFVSLSTPEQEIDRGVLQVRVIE
                     FRAGEVSVKGVPDGEAAYVRSKIALRTGDAIDARELTYDLDGLNRYPFRQVQAAFTPG
                     AALGESDMLLSAHKTRPWQAYGGYGNDGSPSTSFDRYFIGGAVGGLLGRDSVLSMQAT
                     ASRDALAGDKHPHYQSAALNYAIPVGRRGLIEA"
     gene            complement(932..>1968)
                     /locus_tag="VF402_02620"
     CDS             complement(932..>1968)
                     /locus_tag="VF402_02620"
                     /inference="COORDINATES: protein motif:HMM:NF037374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HEX7799214.1"
                     /translation="ILAAASDTKTYDAATGSTGAVGVSGLQGSDTVTGLSQSFDSKNA
                     GSRTLIVNGGYVVNDGDSGGNYAVTTQTASGSIAKAALTAGLTGVVEKVYDATADADL
                     TAANYTLSGVMSGDEVALNAPSAGVYEGKDAGSGRSVSVGGIALTGVDAGNYTVNASA
                     SADIGVIDQRPITVAADDLGKIVGAADPALTWKLASGDLVGGDAVTGGLTRASGETPG
                     DYAITQGSLAASSNYKVTFTGATFTITPTGLQNQPIVNPVQNTAAPPPAPAPDNGSGS
                     SASGSSGSDSSASGGSASSASGGEDDPTDPNVHHGSSSSDTCVTGEACSNQPYPGNQQ
                     FSSYVSFLSH"
BASE COUNT          327 a          676 c          654 g          311 t
ORIGIN      
        1 acgcctcgat caggccgcgc cgcccgaccg ggatggcata gttcagcgcc gcgctttgat
       61 aatgtggatg cttgtcgccc gccagcgcgt cgcgcgaagc ggtcgcctgc atggacagca
      121 cgctgtcgcg gcccaaaagg ccgcccacgg cgccgccgat gaaataacgg tcgaagctgg
      181 tcgagggcga gccgtcattg ccgtaaccgc cataggcctg ccacggccgc gtcttgtgcg
      241 cgctcagcag catgtcgctt tcgcccagcg ccgcgccggg cgtgaaggcg gcctgcacct
      301 ggcggaacgg atagcggttg agcccgtcca ggtcataggt tagctcgcgc gcatcgatcg
      361 cgtcgccggt tcgcagcgcg atcttcgagc gcacataggc cgcctccccg tccggcacgc
      421 ccttgaccga cacctcgccc gcgcggaact cgatcacgcg cacctgaagc acgccccggt
      481 cgatttcctg ctccggcgtg gacagggaga cgaacggata gttcaggtcg cgatagtcct
      541 gcgcgacggc ggcctcgatc tgcgcgatca gcttgcgcga cagggtctgg cccagaaacg
      601 gccgcaggcg cgcggcgatg tcggcccgcg ccttgtccgc gcgcagccgg tccacctcgc
      661 cgaccgtgac gccgggcgcg gcgccgtcat gcgcggcttc gaccggcccc agcagcacga
      721 tcgcgctcag ggccgggccg atcggcgcgg cgtcctgatc gttcggcacg gcgttgggcg
      781 tgaccagcgc gcgggtcgtg acctgcggcg tttccggcag gtggcgctcg atggcctggg
      841 cgtgcgcctg aaccggcgcc tgagcggcgg ccagcagcaa cggggacagg ctgagggcgg
      901 cccggcggaa ggtcttgatc gaggacatga tttagtggct cagaaagctg acataggagg
      961 agaattgctg attgcccgga tagggctggt tcgagcaggc ctcgcccgtg acgcaggtgt
     1021 cggacgagga cgaaccgtga tggacattcg gatcggtcgg gtcgtcctcg ccgccggaag
     1081 cgctggaagc ggaaccgcct gaagccgaac tatctgaacc tgaactcccg gaagccgaac
     1141 tgcctgaacc gttatccggc gccggcgccg gcggaggcgc cgccgtgttc tgcaccgggt
     1201 tgacgatcgg ctggttttgc agacccgtcg gcgtaatggt gaaggtcgcg ccggtaaagg
     1261 tgaccttgta gttcgacgaa gccgccagcg agccctgcgt aatcgcgtaa tcgcccggcg
     1321 tctcgcccga agcgcgcgtc aggccgccgg tgaccgcgtc cccgccgacc agatcgccgg
     1381 aggccagctt ccaggtcagg gccgggtcgg cggcgcccac gatcttgccg agatcgtccg
     1441 ccgcgacggt gatcggacgc tgatcgatca cgccgatatc ggccgaggcc gaagcgttga
     1501 ccgtataatt gcccgcatcg acgcccgtaa gcgcgatgcc gccgacgctg acgctccggc
     1561 cggaccccgc gtccttgccc tcatagacgc cggcggaagg cgcgttaagc gccacctcgt
     1621 ccccgctcat cacgccggac aaggtatagt tggccgcagt caaatcggcg tccgcagtcg
     1681 cgtcataaac cttctcgacc acgccggtca gcccagccgt caacgccgcc ttggcgatgg
     1741 agcccgaagc cgtctgcgtc gtcaccgcat agttcccgcc gctgtcgcca tcattgacga
     1801 cataaccgcc gttgacgatg agcgttcgag aaccagcgtt cttcgagtcg aacgactgcg
     1861 ataggccggt aaccgtatcg gagccttgca agcccgaaac gccaaccgcg cccgtcgaac
     1921 ccgttgccgc gtcataagtc ttcgtatcgc ttgccgccgc caaaatca
//
LOCUS       DASVLL010000158         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_62743, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000158 DASVLL010000000
VERSION     DASVLL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62743"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..772
                     /locus_tag="VF402_02625"
     CDS             80..772
                     /locus_tag="VF402_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX7799215.1"
                     /translation="MSERPSPRISSRKSPKQARSSELVAAILQAAAQVLAQDGAPRFT
                     TARVAEKAGVSIGSLYQYFPNKAAILFRLQSDEWRQTSGLLHVILTEQGLPPPERLRR
                     LTHAFVRSECEEAGLRVALHDAAPLYRAAPEARAVRAQGETGMQIFMREALPDAGEAA
                     RILAGELIAATLSAVGKQFSETPRTDAEIETYAGALADMLCAYLDKAASGTTCAIAAS
                     PAALGCRWSPES"
     gene            complement(685..1902)
                     /locus_tag="VF402_02630"
     CDS             complement(685..1902)
                     /locus_tag="VF402_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7799216.1"
                     /translation="MKARAYGFALAGLLFVLPGLSCAQDWVPTWQASPAPSDKAAQTV
                     SDLTLRQIVHVSAGGDSVRVRLSNAYGKAPVTFDAAAVALRAEGAAIRAGSSRPLTFS
                     GKASVTIPAGAYVLSDPAGLDVAQGADLAIDLHVAGPVAPDTIHVIQRDAVYAAQGDV
                     VGRTDLTAIKPPADGLWLWLSEVEVSGGPAKSAVVAFGDSITDGFRQKPDADTAWPDA
                     LAARLAAAGTPMGVINAGISGNRLLHDGQWPPFGAAGLTRFDRDVLAQPNVSSVIVLL
                     GINDIGQVTKGADDPDYVSAEAIEAGLTQLAERAHERGLKAYVATLTPFRDTTIKNYY
                     DDGKEAIRVAVNAWIRGQTAFDGVIDFDKAIEDPARPGHMRAAYDSGDHLHPNAAGDA
                     AMAQVVPLAALSR"
BASE COUNT          340 a          744 c          686 g          323 t
ORIGIN      
        1 aagaacggcg cgggcttgcg ccggaggtaa aaatacgaat aattgttcgc gttttctaat
       61 cgcattgccg gaccgcacca tgagcgagcg cccaagtccc cgtatttctt cacgaaagtc
      121 accgaaacag gcgcgctcca gcgagctggt cgcggccata ttacaggcgg cggctcaggt
      181 tctggcgcag gacggcgcgc cgcgcttcac cacggcgcgc gtcgccgaaa aggcgggcgt
      241 cagcatcggc tcgctgtatc agtatttccc gaacaaggcc gcgatcctgt tccggctgca
      301 aagcgacgaa tggcgccaga ccagcggcct gctgcacgtc attctcaccg aacagggcct
      361 gccgccgccg gagcgcctgc gccgcctgac gcacgccttc gtccgctccg aatgcgagga
      421 ggcggggctg cgcgtggcgc tgcacgacgc ggcgccgctg tatcgcgccg cgccggaggc
      481 gcgggccgtg cgcgcccagg gcgagaccgg catgcagatc ttcatgcgcg aggccctgcc
      541 agacgccggc gaagccgcgc gcatcctggc cggggaactg atcgccgcca ccctgagcgc
      601 ggtcggcaag cagttttccg aaaccccgcg cacggacgcg gagatcgaaa cctatgccgg
      661 cgcgctggcc gacatgttgt gcgcctatct cgacaaggcg gccagcggca caacctgcgc
      721 catcgccgcg tcgcctgcgg cgttggggtg caggtggtcg ccggaatcgt aggcggcgcg
      781 catgtggccg ggacgggccg ggtcttcgat ggccttgtcg aagtcgatca cgccgtcgaa
      841 ggcggtctgg ccgcgaatcc acgcattgac ggcgacgcgg atcgcctcct tgccgtcgtc
      901 ataatagttt ttgatcgtcg tgtccctgaa cggcgtcagg gtggcgacat aggctttcag
      961 tccgcgctca tgcgcgcgtt cggccagttg cgtcagcccg gcctcgatcg cttcggcgga
     1021 cacatagtct ggatcgtccg cgcccttcgt cacctgcccg atgtcgttga tgcccaaaag
     1081 cacgatcacg ctcgacacgt tcggctgcgc cagcacgtcg cggtcgaagc gcgtcaggcc
     1141 cgccgcgccg aacggcggcc actggccgtc gtgcagcagg cggttgccgc tgatcccggc
     1201 gttgatcacg cccatcggcg tccccgccgc cgccagccgc gcggccagcg cgtcgggcca
     1261 ggcggtgtca gcgtcgggct tttggcggaa gccgtcggtg atcgaatcgc cgaaggcgac
     1321 gaccgcgctt ttcgccggtc cgccggacac ctcgacctcg gacagccaca gccacagacc
     1381 gtccgcgggc ggcttgatcg cggtcaggtc cgtccgcccg acgacatcgc cctgcgcggc
     1441 atagacggcg tcgcgctgga tgacatggat cgtgtccggc gcgaccggcc cggccacatg
     1501 caggtcgatg gcgaggtccg ccccctgcgc cacatcgagg ccggccggat cggacagaac
     1561 ataggccccc gccggaatcg tcaccgacgc cttgccggaa aaggtcagcg gccgcgacga
     1621 cccggcccgg atggccgcgc cttccgcccg cagcgccacg gcggcggcgt cgaaggtcac
     1681 cggcgccttg ccgtaggcgt tgctcagccg gacgcgcacc gaatcgccgc ccgcgctgac
     1741 atgcacgatc tgacgcaggg tcaggtcgga gacggtttgc gccgccttgt cggacggcgc
     1801 cgggctggcc tgccaggtcg gaacccagtc ctgcgcgcag ctcagtcccg gcaggacgaa
     1861 cagcagcccc gcaagggcga atccgtatgc gcgcgccttc atcttcatcc cgccgtctcc
     1921 gcgtttgtcg tcccgccaac gcttaaagcc atcccatcgg gacggcaagc cacaggcgcg
     1981 ataacgacgt ttttcatcgc atctccttgt caatgacacc ggtttccttt acacctgccg
     2041 ccaagacgcc gacaacaggc gcgcgaacat caacaggcgg caggactcca acg
//
LOCUS       DASVLL010000159         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_439844,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000159 DASVLL010000000
VERSION     DASVLL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439844"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VF402_02635"
     CDS             <1..756
                     /locus_tag="VF402_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7799217.1"
                     /translation="PAPLPAPRGEIRFEHVCFAYPSRPEAVVLDDFSLHVKPGETVAL
                     VGPSGAGKSSVFRLLLRYYNFTEGRIELDGVDIRACDPGAVRTRLSLVAQDAALFSGS
                     AADNIRFGREGATDEEVRAAAAKAQALGFIEALPQGFDTPLGERAKSLSGGQKQRLSI
                     ARALVRDAPVLLLDEATSALDAENERLVQAALGEAMRDHTTLVIAHRLATVLKADRIV
                     VMEAGRVVDVGTHDELVARGGLYQRLASLQFET"
     gene            764..1129
                     /locus_tag="VF402_02640"
     CDS             764..1129
                     /locus_tag="VF402_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7799218.1"
                     /translation="MSDDAQTLTPILPCNDVQASLAFYLRLGFTVYWRDESDPQGYVI
                     LGHPSGAALHLNKAVEGWLVPGRNPFGLYLYVENVDEMAAAVGVAAEDKPWGMYEAAV
                     SDPDETLVRIGWPTSKRGV"
     gene            1208..1900
                     /locus_tag="VF402_02645"
     CDS             1208..1900
                     /locus_tag="VF402_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX7799219.1"
                     /translation="MKVAYPFLLIGLMAASGAAFSAQAADKPFLKLTGEASVATAGMT
                     KGASDTNGDPQGLIGLKVSHGVWFAGARIKNVKTENGDDQQEWYAGAAGKAGGYDLSL
                     QLVYKVIDGTPAGTDNAYYEWQGAVGRSFGKTSVKLSEQYTGDSAGKTRASYWTEASV
                     AQKLTPKISVSAAYGHRDVTPAKNYNGWNVGGTYALLKATSLDVRWYDTDEHGYGDKY
                     AGRLVAKISQKF"
BASE COUNT          360 a          704 c          743 g          356 t
ORIGIN      
        1 ccggcgccgc ttccggcccc gcgcggtgag atccgcttcg agcatgtatg cttcgcctat
       61 ccgtcgcggc ccgaggcggt ggtgctggac gatttcagcc tgcatgtgaa gcccggcgag
      121 acggtggcgc tggtcgggcc gtccggcgcg ggcaagtcga gcgtattccg gcttttgctg
      181 cgttattaca acttcaccga gggccgcatc gaactggacg gcgtcgatat tcgcgcctgc
      241 gatccgggcg cggtgcggac gcgcctgtcg ctggtcgcgc aggacgcggc gctgttctcc
      301 ggctcggcgg cggacaatat ccgtttcggc cgcgaaggcg ccacggacga ggaggtccgc
      361 gccgccgccg ccaaggcgca ggcgctgggc ttcatcgagg ccctgccgca aggcttcgac
      421 acgcctctgg gcgagcgcgc caagtcgctg tctggcgggc agaagcaacg gctttccatc
      481 gcccgcgccc tggtgcgcga cgcgccggtg ctgctgctgg acgaggcgac ctcggcgctg
      541 gacgcggaga acgagcggct ggtgcaggcg gcgcttggcg aggccatgcg cgaccacacc
      601 acgctggtca tcgcgcaccg gctggccacc gtcctcaagg ccgaccgcat cgtggtcatg
      661 gaggccgggc gcgtggtcga tgtcggcacg cacgacgagc tggtggcgcg cggcgggctt
      721 tatcagcggc tggccagcct gcaattcgaa acatgaggcc gatatgtccg acgacgccca
      781 gactctgacg ccgatcctgc cctgcaacga cgtgcaggcc agcctggcct tttacctgcg
      841 gctcggcttc accgtgtact ggcgggacga aagcgatccg cagggctatg tcattctcgg
      901 ccatcccagc ggcgcggccc tgcacctcaa caaggcggtc gagggctggc tggttccggg
      961 gcgcaatccg ttcgggcttt atctctacgt cgaaaacgtc gacgagatgg ccgccgcggt
     1021 cggcgtcgcg gccgaggaca agccgtgggg catgtacgag gccgccgtct ccgacccgga
     1081 cgaaaccctt gtgcgcatcg gctggccgac ctccaaacgg ggcgtttaac cgctttctaa
     1141 cgcatgggtg gtacttcctg atgcaggaca accgacagac ggttatccgc gttcagggac
     1201 agtatcaatg aaagtcgcgt acccatttct tcttatcggc ctgatggccg cctccggcgc
     1261 cgcgttcagc gctcaggccg ccgacaagcc ttttttgaaa ctcaccggcg aggccagcgt
     1321 ggcgacggcg ggcatgacca agggcgcctc ggacaccaac ggcgatccgc agggcctgat
     1381 cgggctgaaa gtctcgcacg gcgtctggtt cgcgggcgcg cggatcaaga acgtcaagac
     1441 cgagaacggc gacgaccagc aggaatggta tgcgggcgcg gccggtaagg cgggcggcta
     1501 cgacctgtcg ctgcaactgg tgtacaaggt gatcgacggc acgcccgcgg gcacggacaa
     1561 cgcctattac gaatggcagg gcgcggtcgg gcgcagcttc ggcaagacct cggtgaagct
     1621 gagcgagcaa tataccggcg attcggcggg caagacgcgc gcctcgtact ggaccgaagc
     1681 ctcggtcgcg cagaagctga cgccgaaaat ctccgtctcg gcggcctatg gccaccgcga
     1741 cgtcaccccg gcgaagaatt ataacggctg gaatgtcggc ggaacctatg cgctgctgaa
     1801 ggcgaccagc ctcgacgtgc gctggtacga caccgacgag catggctatg gcgataagta
     1861 cgccggccgt ctcgtcgcca agatcagcca gaagttttag cggcggttct tccccctctc
     1921 ccctccgcga ggggagagga cgaggaacaa ggcgaagccg cagggcctcg ggtgaggggc
     1981 tggcggacgt ttgctcgacc gtttggcagc ccctcacccg atcgccttcg ctgcgctcgg
     2041 ctctcgtcct ctccccatgt gtatggggag aggggaagag cggcgccatg tgggcgggaa
     2101 gggataccgg atctcgccgc gatcggtttg tcctcaagcc ttggccagcc gttccccccc
     2161 ccc
//
LOCUS       DASVLL010000160         4793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_116964,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000160 DASVLL010000000
VERSION     DASVLL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116964"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF402_02650"
     CDS             <1..473
                     /locus_tag="VF402_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX7799220.1"
                     /translation="DLVLAFKHGDRLDLVPMLSRWLERAGRDVLAQADLIVPVPLHPS
                     RLRHRRYNQAAELARPVARRLGRAYAPDVLRRTRAATQAGKSFDARWQAVRDAFAVPE
                     RARAKIAGRRIVLVDDVFTTGATLKACAQTLLGAGAGGVDVCVLARTASRTAAL"
     gene            531..785
                     /gene="grxC"
                     /locus_tag="VF402_02655"
     CDS             531..785
                     /gene="grxC"
                     /locus_tag="VF402_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX7799221.1"
                     /translation="MPKIDIYTKPYCPYCERAKALLEEKGAAFHEIVASNDPALRAQM
                     NARSGRYTYPQIFIDDLHVGGCDDLFALDARGGLDPLLAA"
     gene            793..1638
                     /locus_tag="VF402_02660"
     CDS             793..1638
                     /locus_tag="VF402_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7799222.1"
                     /translation="MTGVLNLALVQLRTPAEQGAALAHARPLILEAARNGAQFVLLPE
                     CANFVEARKENKARRVTDEASDAFVLGVRELARTLRTPVLIGSAIVRAESDPEYPQAP
                     RAANRTLLINAHGDITARYDKIHLFDADTPDGKSYRESAAMRPGAQAVVAETPWGGLG
                     LSVCYDVRFAHLYRALARAGARMIAVPAAFTVPTGKAHWEVLLRARAIETGCFVLAPA
                     QGGLHEDGRTTYGHTMAVSPWGEVIGRLDHDRPAVLEATLDFSEVAAARQALPQLRHD
                     REFGV"
     gene            1663..2130
                     /locus_tag="VF402_02665"
     CDS             1663..2130
                     /locus_tag="VF402_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX7799223.1"
                     /translation="MIRYALKCDVGHAFEAWFESSDGYDAQAARGLVECPMCGSANVG
                     KAIMAPMVRTSRQKESAEDSARRSVAEALHKLRRHVESTHDYVGGDFAHEARDIHNGL
                     APDRPIYGEATPDEVKSLVEDGVPVAPLPVFSPGGAKAPPPVASGAPEGKKLN"
     gene            complement(2238..3074)
                     /locus_tag="VF402_02670"
     CDS             complement(2238..3074)
                     /locus_tag="VF402_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEX7799224.1"
                     /translation="MKRQRSRNRKPSGNSNNPNRAYESNGPEGTKVRGNAQTIYEKYQ
                     QLARDANSSGDRVLAENHLQHAEHYFRMIRQMQPQRPVSEFVQRDPFSNGFDDFDEDL
                     DGEPAPEADNAEAAETQGESGEARDNRNRDRNRDNRERYDRDNRPDNRNDNRNDNRAD
                     NRPGARNDNRDDENGGRRETRRERYERRRAQRLQDQEKNLSSAETDAPLSPSAIASTE
                     PLGAPKAAAPAAPEPDVSHLPAFLRVPTPVEPAPAEAAEDAPKPKRGRRKKTEEVSAE
                     EA"
     gene            complement(3399..4247)
                     /gene="prmC"
                     /locus_tag="VF402_02675"
     CDS             complement(3399..4247)
                     /gene="prmC"
                     /locus_tag="VF402_02675"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7799225.1"
                     /translation="MTTLVKAWTDAQKRLKAAAIDSPAIDARLLLEAATGATRADILT
                     DPYRELTAEQEATLDACLDKREKRMPVARILGRKGFWKLLLNLSEHVLIPRPETECLV
                     DMVLKAYPEGESFSIADLGTGSGAILLSILSERPAAKGLGTDISEEALAVARDNAAHL
                     GIAARAAFLRTSWGDGLHDGGFDVVVSNPPYIRSDVIPTLEPEVKNHDPMLALDGGSS
                     GLDAYEALAPEIFRLLKTGGRTWLEIGFDQSADVEKLMKSAGFEDVATYLDLSNLPRV
                     VAARKP"
     gene            complement(4259..>4793)
                     /gene="ubiG"
                     /locus_tag="VF402_02680"
     CDS             complement(4259..>4793)
                     /gene="ubiG"
                     /locus_tag="VF402_02680"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX7799226.1"
                     /translation="LLSEPMRRMGFAVTGLDASLRNIGTAKAHAAESGLDIRYLNSTV
                     EQLAQGGEVLYDVVLTMEVIEHVADPDAFLKTCASLVKPGGLLFVATLNRTLKAHALA
                     IVGAEYILRWVPRGTHDWRKFLTPEEIAGFLAATPLEPQAPVGVSYNPLNQQWRLSGD
                     ASVNYMVVATYPASAAF"
BASE COUNT          676 a         1511 c         1740 g          866 t
ORIGIN      
        1 gcgacctcgt gctggcgttc aagcatggcg accggctcga tctcgtcccc atgctgtcgc
       61 gctggctgga gcgggcgggg cgggacgtgc tggcgcaggc cgacctgatc gttccggttc
      121 cgctgcatcc gtcgcggctg cgccatcgcc gctacaatca ggccgccgaa ctggcccggc
      181 cggtggcgcg gcggctgggg cgcgcctatg cgccggacgt gttgcgccgg acgcgggccg
      241 cgacccaggc gggcaagagc ttcgacgcgc gctggcaggc ggtgcgcgac gccttcgccg
      301 tgccggagcg cgcgcgggcg aagatcgcgg ggcggcgcat cgtgctggtc gacgacgtgt
      361 tcaccacggg cgcgaccctg aaggcgtgcg cgcagaccct gctcggggcg ggcgccggcg
      421 gggtcgatgt ctgcgtgctg gccagaacgg cgagccggac ggcggcgctt taatcttcgc
      481 ggcaaatcgc tatatagggg gatcagtcgc caaccctttt tgtgtgcgtt atgccgaaga
      541 tcgacatcta caccaagccc tactgcccct attgcgagcg cgccaaggcg ctgctggaag
      601 agaagggggc ggcgttccat gaaatcgtcg cctcgaacga tccggccctg cgcgcgcaga
      661 tgaacgcgcg ttcggggcgt tacacctatc cgcagatctt catcgacgac ctgcatgtcg
      721 gcggctgcga cgacctgttc gcgctggacg cgcgcggcgg gctcgacccg ctgctggcgg
      781 cctgaggcgc gcatgacggg ggtgctgaac ctggcgctgg tgcagttgcg cacgcccgcc
      841 gagcagggcg cggcgctggc ccatgcgcgt ccgctgatcc tggaggccgc gcgcaacggg
      901 gcgcagttcg tgctgctgcc cgaatgcgcc aatttcgtcg aggcgcgcaa ggagaacaag
      961 gcgcggcgcg tgacggacga ggcgtccgac gccttcgtgc tgggggtgcg cgaactggcg
     1021 cggaccttgc gcacgccggt gctgatcggt tcggccatcg tgcgcgcgga gagcgatcct
     1081 gaataccctc aggcgccgcg cgccgccaac cgcaccctgc tgatcaacgc gcacggcgac
     1141 atcacggcgc gctacgacaa gatacacctg ttcgacgccg atacgccgga cggcaagagc
     1201 tatcgcgaaa gcgccgccat gcggccgggc gcgcaggcgg tcgtggccga aacgccgtgg
     1261 ggcggtctgg gcctgagcgt ctgctacgac gtgcgcttcg cgcatcttta ccgcgcgctg
     1321 gcgcgggcgg gggcgcgcat gatcgcggtt ccggccgcct tcaccgtgcc gaccggcaag
     1381 gcgcactggg aggtgctgct gcgcgcccgc gccatcgaga cgggctgttt cgtgctggcg
     1441 cccgcgcagg gcgggctgca cgaggacggg cgcacgacct acggccacac gatggcggtc
     1501 agcccgtggg gcgaggtgat cggacggctt gaccacgacc gcccggccgt gctggaagcg
     1561 acgctcgatt tcagcgaggt cgccgccgcg cgtcaggcgc tgccgcaact gcgccacgat
     1621 cgcgaatttg gagtttaggg gcgccgcccc catatctgga acatgatccg ttacgctttg
     1681 aaatgcgatg tcggccatgc gttcgaggcc tggttcgaat cgtcggacgg ctacgacgcg
     1741 caggccgcgc gcgggctggt cgaatgcccg atgtgcggct cggcgaacgt cgggaaggcg
     1801 atcatggcgc cgatggtgcg cacctcgcgc cagaaggaga gcgccgagga cagcgcccgg
     1861 cgcagcgtgg ccgaggcgct tcataagctg cgccggcatg tcgaaagcac gcacgactat
     1921 gtcggcgggg atttcgcgca tgaggcgcgc gacatccata acggcctggc gcccgaccgt
     1981 ccgatctatg gcgaggcgac gcccgacgag gtgaagtcgc tggtcgagga cggggtgccg
     2041 gtcgcgccgc tgccggtgtt cagccccggc ggggcgaagg ctccgccgcc ggtggcgagc
     2101 ggggcgccgg aggggaagaa gctgaattag gggaggacgc gaggcccctc ccacccttgc
     2161 tgcgcaggga gggcgaatat ctcgccttcc ccgccaaatc aaaacccctc cggcgctgcc
     2221 ggaggggttt ttcagtttca ggcttcctcg gcgctgacct cttcggtctt tttgcggcgg
     2281 ccgcgtttcg gtttgggcgc gtcttcggcg gcctcggccg gggccggttc gacgggcgtg
     2341 gggacgcgca ggaaggcggg caggtggctg acgtccggtt ccggcgccgc gggcgccgcc
     2401 gctttcgggg cgcccagagg ctcggtcgag gcgatggcgc tcggcgagag cggggcgtcg
     2461 gtttcggcgc tgctcaggtt cttttcctga tcctgcaaac gctgggcgcg gcggcgctcg
     2521 tagcgttcgc ggcgggtttc gcggcggccg ccgttctcgt cgtcgcgatt gtcgttacgg
     2581 gcgccgggac ggttatcggc ccgattgtcg ttacggttat cgttacgatt atctggccgg
     2641 ttgtcgcggt cgtagcgttc gcggttgtcg cggttgcgat cccggttgcg gttgtcgcgc
     2701 gcttcgccgg attcgccctg cgtctcggcg gcttcggcgt tgtcggcttc cggcgcgggc
     2761 tcgccgtcca ggtcttcgtc gaaatcgtcg aagccgttgg agaacgggtc gcgctgcacg
     2821 aattccgaca ccgggcgctg cggctgcatc tggcggatca tgcggaaata gtgttcggcg
     2881 tgttgcagat ggttttcggc cagcacccgg tcgccggacg agttggcgtc gcgcgcgagc
     2941 tgctggtact tttcataaat ggtctgggcg ttgccgcgca ccttcgtgcc ttcgggtccg
     3001 ttcgactcgt aggcgcggtt gggattgttg ctgttgccgg acggcttgcg gttacggctg
     3061 cgttgccgtt tcatgccccg aaagtctttc atataactta cgtctctctg cgcgccgcca
     3121 tgatctctgg gctgggcggc gtagcggtta ggggtgtgca aggcgccgtc ggatcttccc
     3181 gcatttacgg ggccttcagt cggcgattaa ccttgtctcg tcttgttgga aaagcgttgg
     3241 ggtcgctgtc ggcccggtta tctctcacgc tctcgcacgt ccctgccgca agaaaaccgc
     3301 gcgtaccgaa ggccgggagg tttttaccgc ttgcgatggg gtgaagacag tttctagtta
     3361 gcgcctcttt gcgcgtctgc caagaggctt tttttgcgtc agggtttgcg cgccgccacc
     3421 acgcgcggca ggttggacag gtcgagatag gtggccacat cctcgaaacc ggctgatttc
     3481 atgagctttt ccacgtccgc ggactggtcg aaaccgatct ccagccaggt ccgtccgccc
     3541 gttttcaaga ggcggaaaat ttccggcgcc agcgcttcat aggcgtccag gccgctcgat
     3601 ccgccgtcaa gggccagcat cggatcgtga ttcttcacct ccggctcgag ggtgggaatc
     3661 acgtcggagc ggatataggg agggttggac accacgacgt cgaagccgcc atcatgcagg
     3721 ccgtcgcccc atgaggtgcg caaaaacgcc gcgcgcgccg cgatgccgag atgggcggcg
     3781 ttgtcgcgcg ccacggccag cgcctcctcg ctgatgtccg tgccgaggcc cttggccgcc
     3841 gggcgctcgc tgaggatcga cagcaggatc gcgccggagc ccgtgccgag gtcggcgatg
     3901 gagaagctct cgccttcggg gtaggctttc agcaccatgt cgaccagaca ttccgtctcc
     3961 gggcgcggga tcagcacgtg ttcggacagg ttcagcagca gcttccagaa gcccttgcgg
     4021 cccaggatgc gcgcgaccgg catgcgcttt tcgcgcttgt cgagacaggc gtcgagggtc
     4081 gcttcctgtt cggcggtcag ttcgcgatag gggtcggtca ggatgtccgc gcgcgtcgcg
     4141 ccggtcgccg cttccagcag caggcgggcg tcgatggcgg gggaatcgat ggcggcggcc
     4201 ttcaggcgtt tctgcgcgtc ggtccaggct tttacgagtg tggtcatggt cttcgggttt
     4261 aaaacgcggc gctggcggga taggtcgcca ccaccatgta gttgacggac gcgtcgcccg
     4321 acaggcgcca ctgttggtta agcgggttgt agctgacgcc gaccggggct tgcggctcca
     4381 gcggcgtggc ggcgaggaag ccggcgatct cctccggcgt gaggaatttg cgccagtcgt
     4441 gcgtgccgcg cggcacccag cgcaggatgt attccgcgcc gacgatggcc agggcgtgcg
     4501 ccttcagcgt gcggttcagc gtggcgacga acaaaagccc gccgggcttg accagcgagg
     4561 cgcaggtctt gaggaaggcg tccgggtcgg cgacgtgttc gatcacctcc atcgtcagca
     4621 cgacgtcgta gagaacctcg ccgccctgcg ccagttgctc gacggtggaa ttgaggtagc
     4681 ggatgtcgag gccgctctcc gccgcgtgcg ccttggcggt gccgatattc ctcaaagagg
     4741 cgtcgaggcc ggtgacggcg aaacccatgc gccgcatcgg ctcggacaac agc
//
LOCUS       DASVLL010000161         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_224152,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000161 DASVLL010000000
VERSION     DASVLL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224152"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VF402_02685"
     CDS             complement(<1..1539)
                     /locus_tag="VF402_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272757.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEX7799227.1"
                     /translation="MLDASRLSFSDVAVFGLYLLGLLAAGLIGRNRALKAREHFLGGQ
                     SASWLRIGFALYATTISATSLVGLTGSSYAHGVSSAFSYEWPAAVVLTLFCAVVLPTY
                     LKSRIFTVPEFLEMRYGRFVRTYVSGLGIGLGVFLDASGSLFAGAVLFQTLFPGWPLW
                     EVCALLAGLAGAFLLMGGLRAVLMVEGVQGLVMVVSCAALAYFTFSAIGGGDPLHGWG
                     RVMREVDPSHLKLILPASDPVMPWTGLVTGVPLIGFYFWCANQVMVQRVLAAKSLDDG
                     RLGSLLAGALKLTSLFLVCLPAAGAGLVFPHLARPDQVFSHILFDILPHGLTGLFLAA
                     CLVSVLSSLSGVYNSVSTLVTMDFVRRLKPELGDAALVHIGKAVTVGVMLISIVWAPQ
                     IVHFKDTLWQYLQAVLCYFVPPIAAVFVAGLFWRRANARGAGLALVAGTTASFALFYA
                     IEIAHALPLHFLVAAMAIFLIAMAALVAGSLSAPPPDMRRVTPLMFTAAVWAEESAHL
                     RTVRW"
     gene            1700..2530
                     /locus_tag="VF402_02690"
     CDS             1700..2530
                     /locus_tag="VF402_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799228.1"
                     /translation="MTHAEQEQADEAALNARLARLRAFAEDIAARLQTLPPPETWLDA
                     ARAARAMQTADRALSLLPETPPPSCEAHASPAAAPAIAGEDKSTASVSPVYGGGGVCA
                     ANDGGGTTPQAAHTLRHHLRACADRLLDAADRIPYPETHLEAARAQRYALTAQAMLRQ
                     LYTTPRPAPVKRPRDAGDFHIFYHAWDDAGGRDDRLPGDPDFRVDNINCIPLPGEDDR
                     DVWEIIEATDREQERADDAEDCGYWPDGVEFDPGDPFYSCPRLQYWAQSHAFTPSAPP
                     "
BASE COUNT          507 a          982 c          829 g          324 t
ORIGIN      
        1 ccagcgcacg gtgcgcaggt gcgcgctttc ctcggcccag acggcggcgg tgaacatcag
       61 cggcgttacg cgccgcatgt ccggcggcgg cgcgctcagg ctgcccgcga ccagcgccgc
      121 catcgcgatc aggaagatcg ccatcgccgc gaccagaaaa tgcagcggca gggcgtgggc
      181 gatctcgatg gcgtagaaca gcgcgaagct ggccgtcgtc ccggccacca gagccagacc
      241 cgcgccgcgc gcattggccc gccgccagaa cagcccggcg acgaagacgg cggcgatggg
      301 cggcacgaaa tagcacagga ccgcttgcag gtactgccac agcgtgtcct tgaaatgcac
      361 gatctgcggc gcccagacga tcgagatcag catgacgccg accgtcaccg ccttgccgat
      421 atgcaccagc gccgcgtcgc ccagctccgg cttcaggcgg cggacgaaat ccatcgtcac
      481 cagcgtcgag acggaattat agacccccga cagcgacgac agcaccgaca ccaggcacgc
      541 ggccagaaac agcccggtca ggccgtgcgg caggatgtcg aacaggatgt gcgaaaacac
      601 ctgatcgggc cgggccagat gcggaaagac gaggcccgcg cccgccgccg gcaggcagac
      661 cagaaacagg ctggtcagtt tcagcgcccc ggccagcagc gagccgagcc gcccgtcgtc
      721 gagcgatttc gccgccagca cgcgctgcac catgacctga ttggcgcacc agaagtagaa
      781 gccgatcaac ggcacgccgg tgacgaggcc ggtccacggc atgaccggat cggacgcggg
      841 caggatcagt ttcaggtgcg acggatcgac ctcgcgcatg acccgccccc agccgtgcag
      901 cggatcgccg ccgccgatgg ccgaaaaggt gaaataggcc agcgccgcgc acgacaccac
      961 catgacgagg ccctgcaccc cctcgaccat cagcacggcg cgcaatcccc ccatgagcag
     1021 gaacgctccg gccagcccgg ccagcagcgc gcacacctcc cacaacggcc agcccggaaa
     1081 cagggtctga aacagcaccg cgcccgcgaa cagcgacccc gaggcgtcga ggaacacgcc
     1141 cagcccgatg cccagacccg acacataggt gcgcacgaag cgcccgtagc gcatctccag
     1201 aaactccggc acggtgaaga tgcgcgattt cagataggtc ggcagcacga cggcgcagaa
     1261 cagggtcagc accacggcgg cgggccattc gtaggaaaag gccgacgaca cgccgtgcgc
     1321 gtaggacgag ccggtcagcc cgaccagcga ggtcgccgaa atggtggtgg cgtagagcgc
     1381 gaagccgatg cgcagccacg aggccgactg tccgcccaga aaatgctcgc gcgccttcaa
     1441 ggcccggttg cgtccgatca gccccgccgc cagcaggccc agcaggtaaa ggccgaacac
     1501 ggcgacgtcg ctgaaactga gacggctggc gtcgagcatg atggcccctg cggtgctcca
     1561 ggtttttgtt ctgtcgctgt tccttgtcca aaaagtgaag ctgcttttcg gggaaacgct
     1621 ctgtcgcgcg aaggtaacgg atttgcgccg ccgcgcaatg ccttatcccc gccgcccgat
     1681 ccggccttat gcttgtcgta tgacgcatgc ggaacaggaa caggcggacg aggcggcgct
     1741 gaacgcgcgg ctggcgcgcc ttcgcgcctt cgccgaggac atcgccgccc gcctgcaaac
     1801 cctgcccccg ccggaaacct ggctggacgc cgcccgcgcc gcccgcgcca tgcagacggc
     1861 cgacagggcg ctgtcgctgc tgccggaaac gccccctccg tcgtgtgagg ctcacgcctc
     1921 acccgctgcc gcccccgcta tcgcagggga ggataagagc accgcatccg tctcccccgt
     1981 ttacggggga ggtggcgttt gcgcagcaaa cgacggaggg gggaccacac cccaggccgc
     2041 tcacaccctg cgccaccact tacgcgcctg cgccgaccgc ctgctcgacg ccgccgaccg
     2101 gattccctac cccgaaaccc acctcgaagc cgcccgcgcc cagcgctacg ccctgaccgc
     2161 gcaggccatg ctgcgccaac tctacacgac ccccagaccc gcccccgtca aacgcccgcg
     2221 cgacgccggg gacttccaca tcttctacca cgcctgggac gacgcgggcg gccgcgacga
     2281 ccgcctgccc ggcgatcccg atttccgcgt cgacaacatc aactgcatcc cgctgcccgg
     2341 cgaggacgac cgcgacgtct gggagatcat cgaggccacc gaccgggagc aggaacgcgc
     2401 cgacgacgcc gaggactgcg gctactggcc cgacggcgtg gaattcgacc ccggcgatcc
     2461 gttctattcc tgtccgagac tgcaatactg ggcgcaaagc cacgctttta cgccttcggc
     2521 gccgccgtga tcgtctcgcg cacgtcccgc ggcgtcagac cgtcctcgcg atagtcgcgc
     2581 agcgccttcg agcccttgcg cttggccagc cgccgtccct gatcgtccag cagcagcgcg
     2641 tg
//
LOCUS       DASVLL010000162         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_385331,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000162 DASVLL010000000
VERSION     DASVLL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385331"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF402_02695"
     CDS             complement(<1..345)
                     /locus_tag="VF402_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX7799229.1"
                     /translation="MLVIYGIKNCDTMQKAFKWLDAHGAAYRFHDYKAEGIDAARLRT
                     WCDRLGWEAVLNRAGTTFRKLPDADKQGLDAGKAVALMQAQPSMIKRPMIEAGGALIA
                     GFKPDAVAKLEAA"
     gene            586..789
                     /locus_tag="VF402_02700"
     CDS             586..789
                     /locus_tag="VF402_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7799230.1"
                     /translation="MANGTVKWFNPTKGFGFIQPEDGGNDVFVHISAVERAGLRSLNE
                     GQKVAYEIAQDRGKSSAVNLQTA"
     gene            905..1267
                     /locus_tag="VF402_02705"
     CDS             905..1267
                     /locus_tag="VF402_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7799231.1"
                     /translation="MVQYLSDRLDLAFTALSDATRRGVLEQLARRDASITDLADRFHM
                     TLTGMKKHIGVLENAGLVATEKVGRVRTCRLGPQALAEETAWLEGYRKLWAARFDELD
                     KIVEDLKQKDRLNERNAD"
     gene            1248..1763
                     /locus_tag="VF402_02710"
     CDS             1248..1763
                     /locus_tag="VF402_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7799232.1"
                     /translation="MSETQTNNAATAPSRYPTSVERASGREVVVRRRFHAPARLVFQA
                     WTTPELFQRWWTPKSSGMTMLSCEMDVRVGGGYRLTFGHANFERPMAFFGKYIEVTPP
                     TRLVWTNDEGEDDGAVTTVTFEEQDGYTEVVLRDLYPSEAALDEAIASGSTGGFSEQF
                     DQLDACLAENR"
     gene            complement(1789..3339)
                     /gene="guaA"
                     /locus_tag="VF402_02715"
     CDS             complement(1789..3339)
                     /gene="guaA"
                     /locus_tag="VF402_02715"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7799233.1"
                     /translation="MSQHEALLILDFGSQVTQLIARRVRECGVYCEIHPFTKAAEALE
                     KLQPRAVILSGSPHSVVDAGPRIDADLFARKLPVFGVCYGEQLICEMLGGKVESGHDR
                     EYGRAEIEVKTASPLFKGLAAPGEKETVWMSHGDRVTALPPGFEVIAVSEGAPFAAIA
                     DEARKVYGVQFHPEVVHTANGAAMIRNFLFEVAGFKGDWSMASFRDEQIAKIRAQVGK
                     GRVICGLSGGVDSSVAAVLIHEAIGEQLTCVFVDTGLLRKNEGEQVASLFRDHYNIPL
                     VHVQAQGLFLGALKGVADPETKRKTIGKLFIDVFDAEAAKIEGAEFLAQGTLYPDVVE
                     SVSPHGGPSTVIKSHHNVGGLPDYMKLKLVEPLRELFKDEVRALGRELGLSEQFVGRH
                     PFPGPGLAIRIPGEVTPEKVRTLQDADAIYLDEIRKAGLYDRIWQAFAVLLPVKTVGV
                     MGDARTYEDVLALRAVTSSDGMTADFYEFPWPVLGRCATRIVNEVRGVNRVVYDVTSK
                     PPGTIEWE"
     gene            complement(3344..4645)
                     /locus_tag="VF402_02720"
     CDS             complement(3344..4645)
                     /locus_tag="VF402_02720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX7799234.1"
                     /translation="MTPAARLQAAAEILDQLAGARLTAEAVLKNWGQANRYAGSKDRR
                     AIADRVYRCLRAKQRLLWAMQAPERGSGRALVLGSLALIDGLPIAEIEALFSGQGYGP
                     APLSAEERQRLTAGEGEAPDWVKSGLPPFVVARFKAQFGDDWAAEATALMSPRAPIDL
                     RVNGLRATRDAMLDVLREAGLEPAPTPFSAFGIRLPSEPPPNLVKLDAYQNGLIEIQD
                     EGSQLAAFIAGSGVLPGAQKVVDFCAGGGGKTLALAQMMAGQGEIWACDVVEKRLRAI
                     EPRLARANQSAHFLHFSDPADEGLLAPLRDADLVLVDAPCSGSGTWRRHPEEAHRLTE
                     DEVRRLHVLQVQILNRAATCVKPGGRLVYVTCSVLDDENAESADAFAAGRPEFAPLDV
                     AALADARYFDSTNLNPVGHRLSLSPHKTQTDGFFLSAWQRR"
BASE COUNT          798 a         1593 c         1504 g          797 t
ORIGIN      
        1 cgcggcttcc agcttcgcca cggcgtccgg cttgaaaccc gcgatcagcg ctccgcccgc
       61 ctcgatcatc ggacgcttga tcatcgacgg ctgcgcctgc atcagggcga cggccttgcc
      121 cgcgtcgagg ccctgcttgt cggcgtcggg cagcttgcgg aaggtggtcc cggcgcgatt
      181 gagcacggcc tcccagccca gtcggtcgca ccacgtcctc aggcgcgccg cgtcgatccc
      241 ctcggccttg taatcgtgaa agcgataggc ggcgccgtgc gcgtccagcc atttgaacgc
      301 cttttgcatg gtgtcgcaat tcttgatgcc gtagatgaca agcatggccg tatcccgttc
      361 gttgccgcgg ctttcgcata acggtccgcc cgcgcccgga caaggcgccg cagacatatt
      421 tgcatccgcc gcatctctat gatagagtac atatattcgg aattcaggtt ttgacttgtc
      481 tgttcgcctg aataggcctc tccaagacat cgctactttc ggatccggtc gcgcgctctt
      541 gcgcgatcga aaatgcaccg aggtgcgata ataaaggagg ccacgatggc caacggtact
      601 gtcaagtggt tcaacccgac caagggtttc ggtttcattc agcctgaaga cggcggcaac
      661 gacgtgttcg tccacatcag cgcggtcgaa cgcgcgggcc tgcgttcgct gaacgaaggc
      721 cagaaggtcg cttacgagat cgcgcaggat cggggcaaga gctcggcggt caatctgcaa
      781 accgcctgat cccgaatcgg aatcagaaga aagcccggtg tttcgccggg ctttttttat
      841 gcctgcgcgc ctgtgccgat atccccgctt gccattcccc tgttatcgcc atatagtgaa
      901 ccatatggtt cagtatttat cggatcgcct cgacctcgcc ttcacggccc tgtccgacgc
      961 cacgcggcgc ggcgtgctgg agcaactggc gcgccgggac gcctcgatca cggacctcgc
     1021 cgacaggttc cacatgaccc tcacgggcat gaagaaacat atcggcgtgc tggaaaacgc
     1081 cggactcgtc gccaccgaaa aggtcgggcg cgtgcgcacc tgccgactgg ggccgcaggc
     1141 gctggccgag gaaacggcgt ggctcgaagg ctaccgcaag ctatgggcgg cgcgcttcga
     1201 cgaactggac aagattgtgg aagacctgaa acagaaggac aggctcaatg agcgaaacgc
     1261 agactaacaa cgccgcgacg gcgccgtcgc gctatccgac cagcgtcgaa cgcgcctccg
     1321 gccgcgaagt ggtggtcagg cgccgctttc acgcgcccgc ccgcctcgtc tttcaggcct
     1381 ggacgacgcc ggagcttttc cagcgctggt ggacgccgaa gtcttcgggc atgaccatgc
     1441 tgtcgtgcga gatggacgtg cgcgtcggcg gcggctaccg cctgacgttc ggccacgcca
     1501 atttcgagcg cccgatggcg tttttcggca aatatatcga ggtgacgccg ccgacgcgcc
     1561 tggtctggac caatgacgaa ggcgaagatg acggcgccgt caccacggtg accttcgagg
     1621 aacaggacgg ctataccgaa gtcgtgctgc gcgacctcta tccgtccgaa gccgcgctcg
     1681 acgaagcgat cgcttcgggc agcaccggcg gattcagcga acagttcgac caactggacg
     1741 cctgcctcgc cgagaaccgg tgacgttgac gaccttcgag ccgcccggtt attcccactc
     1801 gatcgtgccg ggcggcttgg aggtcacgtc atagacgacg cggttgacgc cgcgcacctc
     1861 gttgacgatg cgcgtggcgc agcggcccag caccggccac ggaaattcgt agaagtccgc
     1921 cgtcatgccg tccgatgagg tgacggcgcg cagggccagc acgtcctcat aggtgcgcgc
     1981 gtcgcccatc acgccgaccg tcttgaccgg cagcagcacg gcgaaagcct gccagattcg
     2041 atcgtacagg cccgccttgc ggatttcgtc cagatagatg gcgtcggcgt cctgaagcgt
     2101 cctgaccttt tccggcgtga cctcgcccgg aatgcggatg gcgaggcccg gtccggggaa
     2161 cggatggcgc ccgacgaact gttcggacag ccccaactcg cggcccagcg cccgcacctc
     2221 gtccttgaac aactcgcgca acggttcgac cagtttcagc ttcatatagt cgggcaggcc
     2281 gccgacattg tggtgcgact tgatgaccgt ggacggcccg ccgtgcggcg agacgctttc
     2341 caccacgtcg ggatagagcg tgccctgcgc gaggaactcc gcgccctcga tcttggcggc
     2401 ctcggcgtcg aacacgtcga tgaacagctt gccgatggtc ttgcgcttgg tttcgggatc
     2461 ggccacgccc ttgagcgcgc cgaggaacaa tccttgcgcc tgcacatgca ccagcgggat
     2521 gttgtaatgg tcgcggaaca gcgacgccac ctgctcgccc tcgttcttgc gcagaaggcc
     2581 ggtgtcgacg aacacgcagg tcagttgctc gccgatggct tcgtggatca gcacggcggc
     2641 gaccgaggaa tcgaccccgc ccgacaggcc acagatcacg cgccccttgc cgacctgagc
     2701 gcggattttc gcgatctgct cgtcgcggaa cgacgccatc gaccagtcgc ccttaaagcc
     2761 cgcgacctcg aacaggaagt tgcggatcat ggcggcgccg ttggcggtat gcacgacctc
     2821 tgggtggaac tgcacgccat agaccttgcg cgcctcgtcg gcgatggcgg cgaacggcgc
     2881 gccttccgac acggcgatca cctcgaagcc cggcggcagg gcggtgacgc ggtcgccgtg
     2941 gctcatccac acggtttcct tttcgcccgg cgcggccagc cctttgaaca gcggcgacgc
     3001 cgtcttcacc tcgatctcgg cgcggccgta ttcgcggtcg tggccggact cgaccttgcc
     3061 gcccagcatc tcgcagatca gttgctcgcc gtagcagacg ccgaacaccg gcagcttgcg
     3121 cgcgaacagg tccgcgtcga tgcgcggccc ggcgtcgacc acgctgtgcg ggctgcccga
     3181 caggatcacc gccctgggct gcaatttctc cagcgcttcc gccgctttgg tgaacggatg
     3241 gatttcgcag tacacgccgc attcgcgcac ccggcgcgcg atgagctgcg tgacctgcga
     3301 gccgaaatcg aggatcaaca gggcttcgtg ttgcgacatg agcttacctt cgttgccagg
     3361 cggacaggaa gaatccgtcc gtctgcgtct tgtgcgggct gaggctgagc ctatggccca
     3421 cggggttgag atttgtcgag tcgaaataac gcgcatccgc cagcgcggcc acatccagcg
     3481 gcgcgaattc gggacgcccc gccgcgaagg cgtcggcgct ttcggcgttt tcgtcgtcca
     3541 gcaccgaaca ggtgacatag accagccgcc cgcccggctt cacgcaggtc gcggcgcggt
     3601 tcagaatctg cacttgcagg acgtgcaggc ggcggacctc gtcctcggtc aggcgatgcg
     3661 cctcctccgg gtggcgccgc catgtgccgg agccggaaca gggcgcgtcg accagcacga
     3721 gatcggcgtc ccgcagcggc gccagcaggc cctcatccgc cgggtcggaa aaatgcagga
     3781 aatgcgcgct ctggttcgcg cgcgccagtc gcggctcgat ggcgcgcagg cgcttctcca
     3841 ccacgtcgca ggcccagatc tcgccctgcc cggccatcat ctgcgccagg gccagcgtct
     3901 tgccgccgcc gcccgcgcag aaatcgacca ccttctgcgc gccgggcagc acgcccgatc
     3961 ccgcgatgaa ggccgccagt tgcgagcctt cgtcctgaat ctcgatcagg ccgttctgat
     4021 aggcgtcgag cttgaccaga ttgggcggcg gctcggacgg caggcggatt ccgaaggccg
     4081 agaacggcgt cggcgcgggc tccagcccgg cttcgcgcaa cacgtccagc atggcgtcgc
     4141 gcgtggcgcg caggccgttg acgcgcagat cgatcggcgc gcgcggcgac atcagcgcgg
     4201 tcgcctccgc cgcccagtcg tcgccgaact gcgccttgaa acgcgcgacc acaaacggcg
     4261 gcaggccgga tttcacccag tccggcgcct cgccttcgcc cgccgtcaac cgttgacgct
     4321 cctcggcgga gagcggcgcc gggccgtagc cctgcccgct gaacaaggct tcgatctccg
     4381 ctatcggcaa accgtcgatc agggccagcg accccagcac cagcgcgcgc ccggagccgc
     4441 gttccggcgc ctgcatcgcc cacagcaggc gctgcttcgc gcgcaggcag cggtacacgc
     4501 ggtcggcgat ggcgcggcgg tccttggacc cggcgtagcg gttggcctgc ccccagtttt
     4561 tcagcaccgc ctcggcggtc aggcgcgcgc ccgcaagctg gtcgaggatc tcggcggcgg
     4621 cttgaagtcg ggcggctggg gtcaaacggg cttcctaggc gaacaaggcg gacaggcaca
     4681 tgacgatgcc tg
//
LOCUS       DASVLL010000163         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_493555,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000163 DASVLL010000000
VERSION     DASVLL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493555"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF402_02725"
     CDS             <1..237
                     /locus_tag="VF402_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HEX7799235.1"
                     /translation="TYMTGTLVKMGQKIAAALMGGDPWAWTPYALLWLGLMAGAVCGA
                     ALYGLFGLNSLWAATAFSALALIYATFDRRVLAD"
     gene            complement(234..452)
                     /locus_tag="VF402_02730"
     CDS             complement(234..452)
                     /locus_tag="VF402_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799236.1"
                     /translation="MDRKQNGGERIAPDGGFIKPGYNARIAPLPLDVEPLPRSRFGNA
                     IDVRRLNGSVAVDDDDETILDADEGLKD"
     gene            complement(540..>1566)
                     /locus_tag="VF402_02735"
     CDS             complement(540..>1566)
                     /locus_tag="VF402_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7799237.1"
                     /translation="AYHFDSALYGKYLRGLSEAAGVKQIEGQIVSVQQHPETGFITSV
                     TLASGQVVEGDLFIDCTGFRGLLIEQTLKAGYEDWGHWLPTDSALAVQTRSDAPVTPY
                     TGVYAHGEGWRWRIPLRNRVGNGLIYCSAYLSDDAAMERLHASLNEPMSNEPRLIRYK
                     TGRRKKFWDKNCIAMGLSSGFIEPLESTSIHLFQRSVLHLVQNFPFSGFSDTLANYYN
                     SLSNKELEYARDFVILHYKLNERPEAFWRDRAAMSVPDSLQERIDLFRSSGTVYQGPG
                     ELFAVDSWMQVMLGQRLKPEGWHRLGALMNREEIGGVFDGLKKTVDHTVAQMMTHDAF
                     LAQYVSA"
BASE COUNT          238 a          498 c          531 g          299 t
ORIGIN      
        1 acctacatga ccggcacgct ggtcaagatg gggcagaaga tcgcggccgc gctgatgggc
       61 ggcgatccgt gggcgtggac gccgtatgcg ctgctgtggc tgggcctgat ggcgggcgcg
      121 gtgtgcggcg cggcgctgta cggcctgttc ggcctgaaca gcctgtgggc cgcgaccgcc
      181 ttttccgccc tggcgctgat ctacgccacc ttcgaccggc gcgtgctggc ggattagtcc
      241 ttcagccctt cgtccgcgtc gaggatcgtc tcgtcgtcgt catcgacggc gacgctgccg
      301 ttcagcctgc gcacgtcgat ggcgttgccg aagcggctgc gcggcaacgg ctcgacatcg
      361 agcggcagcg gcgcgatccg cgcgttgtag ccgggcttta taaagccgcc gtccggcgcg
      421 atgcgctcgc cgccgttctg tttcctgtcc atgatcgcct ccttcgtcgg atgaggcgat
      481 tatagccgcg ccgccgtcag gttcggctgc ggatcaggcc gcacgatata aggcgcggat
      541 caggccgaaa cgtactgcgc caggaaggcg tcgtgcgtca tcatctgcgc cacggtgtga
      601 tcgacggtct ttttcaggcc gtcgaagacg ccgccgatct cctcgcggtt catcaaggcg
      661 ccgaggcggt gccagccttc cggcttcagc ctctggccca gcatgacctg catccaggaa
      721 tccacggcga acagctcgcc gggcccctgg taaaccgtgc cggaactgcg gaacaggtcg
      781 atgcgctcct gtaaggagtc gggcacggac atggccgcgc ggtcgcgcca gaaggcttcg
      841 ggccgctcgt tcagcttgta gtgcaggatg acgaagtcgc gggcgtattc cagctccttg
      901 ttcgacaggc tgttgtaata gttggccagc gtgtcgctga agccggagaa ggggaagttc
      961 tgcaccagat gcagcacgct tctctggaac aggtggatgc tggtcgattc cagcggctcg
     1021 atgaagccgc tcgacagccc catggcgatg cagttcttgt cccagaactt cttgcgccgt
     1081 ccggtcttgt agcggatcag ccgcggctcg ttgctcatcg gctcgttcag gctggcgtgc
     1141 aggcgctcca tcgccgcgtc gtccgacagg taggcgctgc aatagatcag gccgttgccg
     1201 acgcggttgc gcaacggaat acgccagcgc cagccttcgc cgtgcgcgta aacgccggta
     1261 tagggcgtga ccggcgcgtc gctgcgcgtc tgcaccgcca gcgcgctgtc ggtcggcagc
     1321 cagtggcccc aatcctcgta accggccttg agcgtctgct cgatcagcag gccgcggaag
     1381 ccggtgcagt cgatgaacag gtcgccttcc acaacctgac ccgaggccag ggtcacggag
     1441 gtgatgaagc cggtttccgg gtgctgctgg acgctgacga tctggccttc gatctgtttg
     1501 acgcccgccg cctcgctcag gccgcgcagg tatttgccgt agagcgcgga gtcgaagtgg
     1561 taggcg
//
LOCUS       DASVLL010000164         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_385393,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000164 DASVLL010000000
VERSION     DASVLL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385393"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF402_02740"
     CDS             <1..216
                     /locus_tag="VF402_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HEX7799238.1"
                     /translation="RGFTRVSLKPGEHREVTFTLSPRDLSFVTHDGVRQLTPGDYTLS
                     LGSGQPDTGVRTSDAPLKIARQVVIKD"
     gene            complement(273..>2120)
                     /locus_tag="VF402_02745"
     CDS             complement(273..>2120)
                     /locus_tag="VF402_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEX7799239.1"
                     /translation="ASAFLIQSPDKTLAMQVSRDGSQFTVRRKGEEIIAPSPLGLIPA
                     DAPDYGGLTPVEARRDHVRQATPLIATKAAATVEDYNRLILTYREKTGLRRRISLEIR
                     AYNEGVAFRYLLPKGQAYKVRDERTAFRFGYDPVCEVSDYTASHESTWRATPVSALKD
                     GAALDIPVVCASASQRTHFAITQSALDGYTGSSLRAAPGGFQVHLAPHPDDAHIAVAA
                     ADGLTSAWRVVMTADRAGDLIPSELVGELAPAPQGDFSWVKPGKAAWDWWSGPTAGEK
                     PSMARYERFIDFAAASGFPYFLIDAGWAYGSTPCCTVDPNTDITRAEPGIDMPELVRY
                     AAERHVGLILWAHWRHIAPRVDQVLDVYQRWGIKGVKIDFTERDDQEMVDFFHKMAAA
                     TAQRHMLLDMHGAYPPAGLERTYPNFITQEGVLGAEYNKFSDRVTPAHNVRLAYTRML
                     LGPMDYTPGGFHNATPQTFKVREVMPMTQSTRGQALAMYVVYDSPLQMVSDDPASYDG
                     QAGFDFIKSVPTAWDETRFLDGTPETCVALARRKGKVWYIGAMTNDEGRRLALPLGFL
                     GKGAYSAKLWQDGADANAVATTTQSVTANDSLTLNLKPAGGAVVVLTPQ"
BASE COUNT          325 a          688 c          758 g          349 t
ORIGIN      
        1 cgcggcttca cgcgcgtgtc gctcaagccg ggcgagcatc gcgaggtgac cttcaccctg
       61 tcgccgcgcg atctgagctt cgtcacgcac gacggcgtgc ggcaactgac gccgggcgac
      121 tatacgctca gcctcggctc cggccagccg gacacgggcg tgcggacgtc ggacgcgccg
      181 ctgaagatcg cgcgtcaggt cgtcatcaag gactgagttg aggggggacc ggccgccgcc
      241 ccgccgtcga ggcggggcgg cggccgtctg ttttattgcg gcgtcagcac cacgacggcg
      301 ccgcccgccg gtttcaggtt cagggtgagg ctgtcgttcg cggtcacgct ctgcgtcgtg
      361 gtcgcgacgg cgttcgcgtc ggcgccgtcc tgccagagtt tcgcgctgta ggcgcccttg
      421 ccgaggaagc cgaggggcag ggcaaggcgg cggccttcgt cgttggtcat cgcgccgata
      481 taccacacct tgcccttgcg gcgggccagc gcgacacagg tttccggcgt gccgtcgaga
      541 aagcgcgtct cgtcccacgc ggtcgggacg gacttgatga aatcgaaacc ggcctgtccg
      601 tcataggacg caggatcgtc ggacaccatc tgaagcgggc tgtcatagac gacgtacatg
      661 gccagcgcct gtccgcgcgt cgactgggtc atcggcatga cctcgcgcac cttgaaggtc
      721 tgcggcgtcg cgttgtggaa gccgcccggc gtatagtcca tcggccccag cagcatgcgc
      781 gtataggcca gccggacatt gtgggcgggc gtgacgcggt cggagaactt gttgtactcg
      841 gcgcccagca cgccttcctg cgtgatgaag ttgggatagg tgcgctccag tcccgcgggc
      901 ggataggcgc cgtgcatgtc gagcagcatg tggcgctgcg cggtggcggc ggccatcttg
      961 tggaagaagt cgaccatctc ctgatcgtcg cgctcggtga agtcgatctt gacgcccttg
     1021 atgccccagc gctgatagac gtccaacacc tgatcgacgc gcggcgcgat gtgccgccag
     1081 tgcgcccaca ggatcaggcc gacatggcgc tcggcggcgt agcgcaccag ctccggcatg
     1141 tcgatgccgg gttcggcgcg cgtgatgtcg gtattgggat cgacggtgca gcagggcgtc
     1201 gatccgtagg cccagcccgc gtcgatcaga aaatagggaa agcccgaagc ggcggcgaag
     1261 tcgatgaagc gttcatagcg cgccatgctc ggcttttcgc ccgcggtcgg gccggaccac
     1321 cagtcccatg ccgccttgcc gggcttgacc caggaaaaat ccccctgcgg cgcgggcgcc
     1381 agctcgccga ccagctccga agggatcaga tccccggcgc ggtcggcggt catcaccacg
     1441 cgccatgcgc tggtcaggcc gtccgccgcg gcgacggcga tatgggcgtc gtcgggatgg
     1501 ggggccagat ggacctgaaa gccgcccggc gccgcgcgca aggaactgcc ggtatagccg
     1561 tccagcgcgg actgggtgat ggcgaaatgg gtgcgttggc tggccgaggc gcacacgacg
     1621 ggtatgtcca gcgcggcgcc gtccttgagc gcggacaccg gcgtcgcgcg ccatgtcgac
     1681 tcgtgcgagg cggtatagtc gctgacctcg cacaccggat cgtagccgaa gcggaaggcc
     1741 gtccgttcgt cgcgcacctt ataggcctgc cccttgggca gcaggtagcg gaaggcgacg
     1801 ccttcgttat aggccctgat ctcaagggag atgcggcggc gcaggcccgt cttctcgcga
     1861 taggtcagga tcaggcggtt ataatcctcc accgtcgcgg ccgccttggt ggcgatcagc
     1921 ggggtcgcct gccggacgtg atcgcgccgc gcctcgacgg gcgtcaggcc gccatagtcc
     1981 ggcgcgtcgg cggggatcag gcccaggggc gagggcgcga tgatctcctc gcccttgcgc
     2041 cggacggtga actgcgatcc gtccctcgac acctgcatgg ccagggtctt gtccggcgac
     2101 tggatcagaa aggcggacgc
//
LOCUS       DASVLL010000165         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_171458,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000165 DASVLL010000000
VERSION     DASVLL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171458"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VF402_02750"
     CDS             <1..335
                     /locus_tag="VF402_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799240.1"
                     /translation="ASMAAPEAGAQTNGGEVVPQTGPAPASAPTAACLRYNSNSWNRL
                     ADNITLGQCAVLLYDGRCVSPGQASYGRWGGKTLRLVPRRVEQSDDNTNFRPLVDQGQ
                     GCSVPMVR"
     gene            complement(355..1296)
                     /gene="ispH"
                     /locus_tag="VF402_02755"
                     /gene_synonym="lytB"
     CDS             complement(355..1296)
                     /gene="ispH"
                     /locus_tag="VF402_02755"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7799241.1"
                     /translation="MTQPALKVLLATPRGFCAGVDRAIQIVERALERYGAPVYVRHEI
                     VHNKHVVDRLKDMGAVFVKELDQCPPDRPVVFSAHGVPKSVPAAAEARAMFYLDATCP
                     LVSKVHVEAERHHAEGREIVLIGHAGHPEVIGTMGQLPPGVVKLIETEAQARAFTPQD
                     PDNLAFVTQTTLSVDDTAGIVAVLKERFPSIRLPHKEDICYATTNRQDAVKAVSADCD
                     LVLVIGSAKSSNSQRLVEVALRGGAKAAHLVDDAGHVDWTWFEGVKTLGVSAGASAPE
                     NLIADLIAAVAERFDAEVIENSGVRETVTFKLPKAVA"
     gene            1487..2164
                     /locus_tag="VF402_02760"
     CDS             1487..2164
                     /locus_tag="VF402_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence domain protein"
                     /protein_id="HEX7799242.1"
                     /translation="MRRILTGVCLGLLVNTTAIALAHAQGQGQGDQSDADRAADRDKK
                     DKEWGNTNLHLKKIKAEGPCPYVKVLYDAARYEEFQDNKEASDMAQWTGQINGVNSDC
                     AYKGTEPIHVAMDISFSLGRGPKAESKDKIYHYWVAVTERDKTVLAKKEFDLPVTFAD
                     GQQAQDVNTRIEDIEIPRADITVSGANFEVLVGFDVTPQMADFNRQGKHFRYVSDAEK
                     KDGADGQ"
     gene            2240..>3504
                     /gene="argS"
                     /locus_tag="VF402_02765"
     CDS             2240..>3504
                     /gene="argS"
                     /locus_tag="VF402_02765"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7799243.1"
                     /translation="MSKDAFADSHDFRHQLTRLAARAFEAEGFDAAAGRVNPSDRPDL
                     ADFQCNGALAVAKSAKANPREVAGRVLAHIGGDERLKAADIAGPGFLNFVVSEAALSQ
                     RANALRADDRLGAPRVAHARKVIIDFGGPNVAKEMHIGHLRTAVIGESLKRVFRFMGD
                     TVVGDAHFGDWGYQMGLLIVALSEEQPDLPYFDAGFEGDYPAGAPVSLDDLGRLYPMA
                     SAKGKADADYRDRARRATKELQAGRRGYRALWRHFVAVSREALERDYAALGVSFDLWN
                     GESDADPYIEPMIEDLKAKGLLVPDQGAQIIRVAREDDKKEIPPLLVVSSEGSAMYGT
                     TDLATIVQRKKEFGPDLALYVVYQRQAGHFEQVFRAAYLAGYAHEGELEHAANGTVNG
                     PDGAPFKTRDGGTLKLHELLAQAIAKATER"
BASE COUNT          605 a         1150 c         1189 g          560 t
ORIGIN      
        1 cggccagcat ggccgcgccg gaagcggggg cgcagaccaa tggcggcgag gtcgtgccgc
       61 agaccggacc cgcgcccgcc tccgcgccga cggcggcgtg cctgcgctat aactcgaaca
      121 gctggaaccg tctggccgac aacatcaccc tggggcaatg cgccgtgctg ctgtacgacg
      181 ggcgctgcgt gtcgccgggg caggcctcct acggccgctg gggcggcaag accctgcgcc
      241 tggtgccgcg ccgcgtcgag cagtcggacg acaacaccaa tttccgcccg ctggtcgatc
      301 aggggcaggg ttgctcggtg ccgatggttc ggtagggctt ttggggaggt atcttcaggc
      361 caccgccttg ggcagcttga acgtcaccgt ctcgcgcacg ccggaatttt cgatcacctc
      421 ggcgtcgaag cgttcggcga cggcggcgat caggtccgcg atcaggttct ccggcgccga
      481 ggcgcccgcc gacacgccca gcgtcttgac cccctcgaac cacgtccagt cgacatggcc
      541 cgcgtcgtcg accagatggg cggccttcgc gccgccgcgc agcgcgacct cgaccaggcg
      601 ctgcgaattg gacgacttgg ccgagccgat caccagcacg agatcgcagt cggcgctgac
      661 cgccttgacc gcgtcctgac ggttggtggt ggcgtagcag atgtcttcct tgtgcggcag
      721 ccggatcgac ggaaaacgct ctttcagcac cgcgacgatg cccgccgtgt cgtccaccga
      781 cagcgtggtc tgggtcacga aggcgaggtt gtcggggtct tgcggcgtaa aggcgcgcgc
      841 ctgcgcctcc gtctcgatca gcttcaccac gccgggcggc aactggccca tcgtgccgat
      901 gacctcgggg tggccggcgt ggccgatcag cacgatctcg cggccctcgg cgtggtggcg
      961 ctcggcctcg acatgcacct tggagaccag cgggcaggtg gcgtcgagat agaacatggc
     1021 gcgcgcctcg gcggcggcgg gcaccgactt gggcacgccg tgcgccgaga acaccaccgg
     1081 acggtcgggc gggcactggt ccagttcctt gacgaacacc gcgcccatgt ccttcagccg
     1141 gtcgacgacg tgcttgttgt gcacgatctc gtggcggaca tagaccggcg cgccgtagcg
     1201 ctccagcgcg cgttcgacga tctggatggc gcggtccacc ccggcgcaga aaccgcgcgg
     1261 cgtggccaga aggactttga gagcgggctg ggtcatgcgc ctccacataa tcgctttcgc
     1321 cgcccggcgc cagcgcgcgc aatacaagcg ccgcactttg gccgcaactg ctccataaaa
     1381 ccctttgaaa agccgcgccg agacgctaca catcctgcca actggatgtt ccgcgacggc
     1441 gggccgtctt cacggatcag gggtcgtctt acctatgtcg gttgtcatgc gtcgtattct
     1501 gacgggggtt tgcctcggtc tgctcgtcaa caccacggcc atcgcgctgg cgcacgccca
     1561 gggtcagggg cagggcgatc aaagcgacgc cgaccgggcc gccgaccgcg acaagaagga
     1621 caaggagtgg ggcaacacca acctgcacct taaaaagatc aaggccgaag gcccgtgccc
     1681 ctatgtcaag gtgctgtacg acgccgcgcg ctacgaggag ttccaggaca acaaggaagc
     1741 ctcggacatg gcgcagtgga ccggccagat caacggcgtc aattccgact gcgcctataa
     1801 ggggaccgag ccgatccacg tcgcgatgga catctccttc tcgctggggc gcggccccaa
     1861 ggccgagagc aaggacaaga tctatcacta ctgggtggcg gtgaccgagc gcgacaagac
     1921 cgtgctggcg aaaaaggaat tcgacctgcc cgtgaccttc gccgacggcc agcaggcgca
     1981 ggacgtcaac acccgcatcg aggatatcga gattccgcgc gccgacatca cggtttcggg
     2041 cgccaatttc gaggtgctgg tcggtttcga cgtcacgccg cagatggccg acttcaaccg
     2101 tcagggcaag catttccgct atgtcagcga cgccgagaag aaggacgggg cggacggcca
     2161 gtaacggccg tttttccttg tcaggcgcgg gggcggcgct tatgacggcg aagatttttc
     2221 aattttcgcc cctgagatca tgtccaaaga cgccttcgcc gattcgcacg attttcgtca
     2281 tcaactgacc cgtctggccg cgcgcgcctt cgaggccgag ggcttcgacg cggcggcggg
     2341 ccgggtcaat ccgtccgacc gtcccgacct ggccgatttt cagtgcaacg gcgcgctggc
     2401 cgtcgccaag tcggcgaagg ccaatccgcg cgaggtcgcc gggcgcgtgc ttgcccatat
     2461 cggcggcgac gaacggctga aagcggcgga catcgccggg ccgggtttcc tcaacttcgt
     2521 cgtctcggaa gcggcgctga gccagcgcgc caacgccctg cgcgcggacg accgcctcgg
     2581 cgcgccgcgc gtcgcgcatg cgcgcaaggt gatcatcgac ttcggcggcc cgaacgtcgc
     2641 caaggagatg catatcggcc acctgcgcac ggcggtgatc ggcgaaagcc tcaagcgcgt
     2701 gttccgcttt atgggcgaca cggtggtcgg cgacgcgcat ttcggcgact ggggctatca
     2761 gatggggctg ctgatcgtgg ccctgtcgga ggagcagccg gacctgccgt atttcgacgc
     2821 cgggttcgag ggcgactacc cggccggggc gccggtaagc cttgacgatc tgggccgcct
     2881 ctatccgatg gcctcggcca agggcaaggc ggacgccgat tatcgcgacc gcgcgcgccg
     2941 ggcgacgaaa gaacttcagg ccggacggcg cggctaccgc gctttgtggc ggcatttcgt
     3001 cgccgtgtcg cgtgaggcgc tggagcgcga ttacgcggcg ctgggtgtaa gcttcgacct
     3061 gtggaacggc gaatccgacg ccgatcctta tatcgagccg atgatcgagg atctgaaagc
     3121 caagggcctg ctggtccccg atcagggcgc gcagatcatc cgcgtggcgc gcgaggacga
     3181 taaaaaggag attccgccgc tgctggtcgt gtcgtcggaa ggctcggcca tgtacggcac
     3241 cacggacctc gccaccatcg tgcagcggaa aaaggaattc ggcccggacc ttgcgctcta
     3301 tgtcgtctat cagcgtcagg ccggacattt cgagcaggtg ttccgggcgg cgtatctggc
     3361 cggctatgcg catgagggcg aactggagca cgccgccaac ggcacggtca acgggccgga
     3421 cggcgcgccg ttcaagacgc gcgacggcgg cacgctgaaa ctgcacgaac tgctggcgca
     3481 ggcgatcgcc aaggccaccg agcg
//
LOCUS       DASVLL010000166         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_440307,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000166 DASVLL010000000
VERSION     DASVLL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440307"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VF402_02770"
     CDS             complement(<1..142)
                     /locus_tag="VF402_02770"
                     /inference="COORDINATES: protein motif:HMM:NF023264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTA-gp10 family protein"
                     /protein_id="HEX7799244.1"
                     /translation="MRPCNPARGEVAAAIGGREARLCVTLGALAALEGHFGVSGFAAL
                     GER"
     gene            complement(129..542)
                     /locus_tag="VF402_02775"
     CDS             complement(129..542)
                     /locus_tag="VF402_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEX7799245.1"
                     /translation="MAVQAGRDMLLKISDGGETPVFQTVAGLRARTVSLNAKTVDATD
                     SDSGGWRELLAGGGVKSLAVSGSGVFRDAASDALMQAAFFAQDARGWQIVVPGFGVFS
                     GAFLIAALEYAGQHDGEATFAISLASTGEVSFAAV"
     gene            complement(603..1016)
                     /locus_tag="VF402_02780"
     CDS             complement(603..1016)
                     /locus_tag="VF402_02780"
                     /inference="COORDINATES: protein motif:HMM:NF022810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEX7799246.1"
                     /translation="MTLDPMRDLQGALLAFLRAQDSLTAWLGTPPRVWDQPPGEPVFP
                     YVTFGRGRMQAIGGVDAEVAEQTLNLMCVSRFGGAEEARAVAGALRALLDGAALSLSD
                     QVLCSLRVVFVDVFRAADMRTTYALIRLRAVSEPK"
     gene            complement(1033..1350)
                     /locus_tag="VF402_02785"
     CDS             complement(1033..1350)
                     /locus_tag="VF402_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799247.1"
                     /translation="MSLTARDVRTPAGVYALDEVETDFGGRVRDLTITATVWGDFRPG
                     APGVETPPDGQPFVSQTADFICRSAEGLSVGGWLNARGYDWRIASLDEIADGQTRLRL
                     ERL"
     gene            complement(1347..1616)
                     /locus_tag="VF402_02790"
     CDS             complement(1347..1616)
                     /locus_tag="VF402_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HEX7799248.1"
                     /translation="MTDAVSLDEAKVFLRVSHTAEDELIAMLIAAAQTKLAGETGMVM
                     DENAPADLRLCALYLIAQAYDGRGETGFDPAALEPWIAPYREARL"
BASE COUNT          276 a          658 c          474 g          234 t
ORIGIN      
        1 gccgctcgcc cagcgccgcg aaacccgata cgccgaaatg accttccagc gccgccagcg
       61 cgcccagcgt cacgcacaga cgcgcctcgc gcccgccgat cgccgccgcg acctcgccgc
      121 gcgccggatt acacggccgc aaagctcacc tcgcccgtcg aggccagcga gatggcgaac
      181 gtcgcctcgc cgtcgtgctg cccggcatat tccagcgccg cgatcaggaa cgccccggaa
      241 aacacgccga agcccggcac cacgatctgc cagccgcgcg cgtcctgcgc gaagaacgcc
      301 gcctgcatca gcgcgtcgga cgccgcgtcg cgaaacaccc ccgaacccga cacggccagc
      361 gacttcaccc cgccgcccgc cagcagttcg cgccacccgc ccgagtccga atccgtcgca
      421 tcgaccgtct tggcgttcag cgacacggtc cgcgccctga ggcccgccac cgtctgaaac
      481 accggcgtct cgccgccatc ggaaatcttg agcagcatat ccctgcccgc ctgcacagcc
      541 atctgtttct ccttggtttt tttacgctaa cgctgcgcgc cttgagcgca gcggcgcggc
      601 tcttacttag gttcggaaac cgccctcaaa cggatgagcg cataggtcgt gcgcatatcc
      661 gccgcgcgga acacatcgac gaacaccacg cgcagactgc acaggacctg atcgctcagg
      721 ctcagcgccg cgccgtccag cagcgcgcgc aaggcgcccg ccaccgcccg cgcttcctcc
      781 gcgccgccga agcgcgacac gcacatcagg ttcagcgtct gctcggcgac ctcggcgtcg
      841 acaccgccga tcgcctgcat ccgcccgcgc ccgaaggtca cgtagggaaa gaccggctcg
      901 ccgggcggct ggtcccagac gcgcggcggc gtccccagcc aggcggtcag gctgtcctgc
      961 gcgcgcagga aggcgagaag cgcgccctga aggtcgcgca tcggatcgag cgtcatggtc
     1021 atgtctcttg ttttaaagcc gttccagccg cagccgcgtc tggccgtcgg cgatctcgtc
     1081 gaggctcgcg atgcgccagt cgtaaccgcg cgcattgagc cagccgccga cgctcagccc
     1141 ctccgccgag cggcagatga aatccgccgt ctggctgacg aagggctggc cgtccggcgg
     1201 cgtttccacc cccggcgcgc cgggccggaa atcgccccac accgtcgccg tgatggtcag
     1261 gtcgcgcacc cgcccgccga agtccgtctc gacctcgtcc agcgcataga ccccggcggg
     1321 cgtgcgcaca tcgcgcgccg tcaggctcat agccgcgcct cgcgataagg cgcgatccac
     1381 ggctccagcg ccgccggatc gaaccccgtc tcgccgcgcc cgtcataggc ctgcgcgatc
     1441 aggtaaagcg cgcacagccg caaatccgcc ggcgcgttct catccatgac catgcccgtc
     1501 tcgccggcca gcttcgtctg agccgccgcg atcagcatcg cgatcagctc atcctccgcc
     1561 gtatgcgaca cgcgcaaaaa caccttcgcc tcatccagcg acaccgcatc tgtcatcgaa
     1621 attcgtcctt acatttcttg ta
//
LOCUS       DASVLL010000167         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_171618,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000167 DASVLL010000000
VERSION     DASVLL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171618"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VF402_02795"
     CDS             <1..254
                     /locus_tag="VF402_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799249.1"
                     /translation="WTAGAGVEWMVLPKWSIKAEYLYYNLGTANTGNVGPLFYTSTTA
                     GYAGGSVVAHTGLFDGHVVRLGLNYHFNKAGPAPIVAKY"
     gene            complement(515..1162)
                     /locus_tag="VF402_02800"
     CDS             complement(515..1162)
                     /locus_tag="VF402_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799250.1"
                     /translation="MRPSTENLNAASLTTRAESKNALPPTVWKTNASAPAGGHEKPLI
                     SSSVLRSLVRESPYIAMSVTALIGVGVMSFTGQSLSFYWLILTPLYGAMCIVAGWPRA
                     QSPEQRFRLIWKQALHWFAFLIAMLLVASPEVRGVENNNAAGLNLMTILASGAFVAGV
                     HAEAWQISLVGALLALSVPAIAWIEQSAFLLLLVVALAVFVLAMILWSLRREKSR"
BASE COUNT          352 a          560 c          545 g          315 t
ORIGIN      
        1 gttggacggc gggcgcaggc gtcgaatgga tggtgctgcc gaaatggagc atcaaggccg
       61 agtatctcta ctataacctc ggcacggcga acacggggaa cgtcggccca ttgttctaca
      121 ccagcaccac cgctggctac gccggcggct cggtcgtggc ccatacgggc ctgttcgatg
      181 ggcatgtcgt ccgcctgggc ttgaactacc acttcaacaa ggccggtccg gcgcccatcg
      241 tcgccaaata ttgagagtgc ggcgcaacaa cgcctggggt ttgcaacacg cgtcggccaa
      301 atcgagatgg ctctcatgcg cgcctgtaag ctcgcggtcc atgggcgcgc ctggatcgcg
      361 agccctgcgg cgcgctcgcc gccctcgccg aagcggcatc tgcatccgct tcggcgccaa
      421 gcgctcgaag acgctccaag ggttttgtat cggcggctcc gccatggctg ggaggccggc
      481 gcccttgaag agcctccgga agcgaagcaa gatttcagcg gcttttttct cgacgcagcg
      541 accataagat cattgcgagc acgaaaaccg ccaacgcgac gacgagcagg aggagaaaag
      601 ccgattgctc gatccacgct atcgccggga ccgaaagggc caacaacgct cccacaaggg
      661 agatctgcca cgcttcggcg tggactcccg ctacgaaagc gcccgaggcc aagatcgtca
      721 tgagattgag gcctgcggcg ttgttgttct ccacgccgcg gacttctggc gaggcgacaa
      781 gaagcatcgc gatcaggaag gcgaaccaat gaagagcctg cttccaaatc aagcggaagc
      841 gctgttccgg gctctgagcg cgcggccatc ccgcaacaat gcacatggct ccatataacg
      901 gagtcaatat caaccagtag aagctcaggg actgccccgt gaagctcatg acgcccacgc
      961 cgatcaacgc cgtcaccgac attgcgatgt aaggcgattc cctaactagg ctccgtaaga
     1021 cggatgagct gattagcggc ttttcatgac cgccggcggg cgccgacgca ttcgtcttcc
     1081 aaactgttgg cggcagggca ttcttggatt ctgcacgcgt cgtcagactc gcggcgttga
     1141 ggttttcagt ggaggggcgc atggctcaag acctttttcg gcgcatcttt tttgggcccg
     1201 gtagagatat ggggcgtggc cgcggcgacc gccagactgc gacgcgcttc gccaacccga
     1261 cggcctctgc ttttttgtgc gttttgaatg ggccggcggg ctggaaaatt gggcccgcat
     1321 cacggtccac aagattatat cgtgacgcga tacagcgccg cgcgcagtac ggcgccttcg
     1381 ggtcgcccag cgcgcgaccc atgcggcgaa gaacatccgc acgtttgctc ctgcttcgag
     1441 actgggaacc gaggggatgc ggctcgcacc cctttcaccg cgcgcggcgt tgcatccgac
     1501 attgcatccg gacgggcgta acgctgtctc ggcttccggc ttgccgaaga aagcgggatg
     1561 attctcacac taacacgctg atcgcctatg ccgcgcgcgc cggcgccatc gcaggggacg
     1621 gctcgggtca aaacagcctc ttcacggcgg cgctgccgca taatctcacg cgcacgcccg
     1681 agtgcgactt cgcaattttg cggcccgcgc aagaaaacgg ccacgtcata atcggaatcg
     1741 gggcgcggat cgccccgcgc gcgatccaaa ca
//
LOCUS       DASVLL010000168         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_385747,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000168 DASVLL010000000
VERSION     DASVLL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385747"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1424)
                     /locus_tag="VF402_02805"
     CDS             complement(216..1424)
                     /locus_tag="VF402_02805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7799251.1"
                     /translation="MSTFTESETLKRVKPSPTLAVTAKARELARQGKKVISLGAGEPD
                     FDTPENICDAAIKAIKRGETRYTDVDGILELKQAIVDKFKRENGLEYKTSQIHVAPGG
                     KPVIYNAMMASLNPGDEVIVPAPYWVSYPDMVLLAGGTPVVAVGEMETGFKLKPETLE
                     AAITPKTKWLILNSPSNPSGAAYTADELRALGEVLKRHPQVWVLTDDMYEHILYDGFE
                     YATIAQVVPELYDRTLTMNGVSKAYSMTGWRIGYCAAPEGLIKAMGKIESQSTSNPCS
                     IAQWAAVEALNGPQDFIPQRAAVFQKRRDLVVAMLNNAEGVTCPTPEGAFYVYPSIAG
                     CIGKQTPSGKIIDSDETFASELLQTEEVAVVHGAAFGLSPFFRISYATSEAVLTEACS
                     RIQRFCASLK"
     gene            1787..>2271
                     /locus_tag="VF402_02810"
     CDS             1787..>2271
                     /locus_tag="VF402_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7799252.1"
                     /translation="MWRNLTRFLVNLDARAVRAVWISALLLALVAATFMIGKSPWGRD
                     MTAALETWMESYQHSPLAVVIVTAVFCVSALFGAPQFVLIAACVVAFGPVRGFSYSWF
                     ATVVSAAMTFYMGRFAGQKLLARFGGKRLDRLSGYLGQNAFSASFIVRNIPSAPFIVV
                     N"
BASE COUNT          347 a          711 c          757 g          456 t
ORIGIN      
        1 ccggtcagca ggatatgagg cggcaaaagc cacaccgcgt tcggattatg cgtcatggaa
       61 atgcgtcatg gaaatctgta tctcccgttc ccccaaagcc gccccccggc gtctccttca
      121 gagatatgtc atgcacgtcc gcgcaacgga cgtgaataag cggcgacaga aggctccgcc
      181 gccgcttttg tcaagtcacg cgtttgcgcg cagtcttatt tcaggctggc gcagaagcgc
      241 tgaatccgcg agcaggcctc ggtcagcacc gcttcggagg tcgcgtacga aatgcggaag
      301 aacggcgaca ggccgaaggc ggcgccgtgg acgacggcga cttcctcggt ttgcagcagt
      361 tcggacgcga aggtttcgtc gctgtcgatg atcttgcccg acggcgtctg cttgccgatg
      421 cagcctgcga tcgacggata gacgtagaag gcgccttccg gcgtcgggca ggtcacgccc
      481 tcggcgttgt tcagcatggc caccaccagg tcgcggcgct tctggaacac ggcggcgcgt
      541 tgcgggatga agtcctgcgg tccgttgagc gcctcgacgg cggcccattg cgcgatcgag
      601 cagggattgg aggtcgattg cgactcgatc ttgcccatcg ccttgatcag accttccggc
      661 gcggcgcagt agccgatgcg ccagccggtc atcgaatagg ccttggacac gccgttcatg
      721 gtcagggtgc ggtcatagag ttccggcacg acctgcgcga tggtggcgta ctcgaagccg
      781 tcgtacagga tatgctcgta catgtcgtcg gtcagcaccc agacctgcgg gtggcgcttc
      841 agcacctcgc ccagcgcgcg cagctcgtcg gcggtgtagg ccgcgcccga cgggttggac
      901 ggcgagttga ggatcagcca cttggtcttc ggcgtgatcg cggcttcgag ggtttccggc
      961 ttcagcttga agcccgtctc catctcgccg accgcgacca ccggcgtgcc gccggccagc
     1021 agcaccatgt cgggatagga cacccagtag ggcgccggca cgatcacctc gtcgccgggg
     1081 ttcagcgacg ccatcatggc gttgtagatc accggcttgc cgcccggcgc gacgtggatc
     1141 tggctggtct tgtactccag gccgttctcg cgcttgaact tgtcgacgat ggcctgcttc
     1201 agctccagga tgccgtcgac gtcggtgtag cgcgtctcgc cgcgcttgat cgccttgatc
     1261 gcggcgtcgc agatgttttc cggcgtgtcg aagtccggct cgcccgcgcc gaggctgatg
     1321 accttcttgc cttggcgggc cagttcgcgc gccttggcgg tcacggccag cgtcggggac
     1381 ggcttgacgc gcttgagggt ttcggattcg gtgaaggtcg acatgatggt gctcggctgc
     1441 tctgcggacc cggacggggt cttgcgtgga aaatgcaggc ccctctttac tcgcccgcgc
     1501 cggtttgtcc aaccgtcatc cgtcaacata acgcaaaatc tcgacgcggc gccgcaagtg
     1561 cgtccccggc gccgcgcttg gttgcgtgtg atccggtttc cggagacttt ggcgaagggc
     1621 agggcgggaa ggcgccgccg ccggaggcgt taacggaagg agggctttag caaatcgccg
     1681 ctatggctcc cggcggacgg ctctgaaaaa aaccgattca atctcaaccc attctgctct
     1741 aaggactagg tctcaggccg tcgaggcgca ggtgagttca ggacgtatgt ggcggaatct
     1801 aacgcgcttt ctggtcaatc tcgacgcgcg cgccgtgcgg gcggtgtgga tcagcgcgct
     1861 gctgctggcc ctggtcgccg cgaccttcat gatcggcaag tcgccctggg ggcgcgacat
     1921 gacggcggcg ctcgaaacgt ggatggagtc ctatcagcat tcgccgctgg ccgtggtgat
     1981 cgtgacggcg gtgttctgcg tctcggccct gttcggcgcg ccgcaattcg tgctgatcgc
     2041 cgcctgcgtg gtggcgttcg ggccggtgcg gggcttcagc tattcgtggt tcgccaccgt
     2101 cgtgtcggcg gccatgacct tctatatggg ccgtttcgcc gggcagaagc tgctggcgcg
     2161 cttcggcggc aagcggctgg accgcctgtc cggctatctg ggccagaacg ccttttccgc
     2221 ctccttcatc gtgcgcaaca tcccctcggc gccgttcatc gtcgtcaaca t
//
LOCUS       DASVLL010000169         5652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_440546,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000169 DASVLL010000000
VERSION     DASVLL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440546"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VF402_02815"
     CDS             complement(<1..502)
                     /locus_tag="VF402_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799253.1"
                     /translation="MIVDDSAVVRGLISRWLEQEPDMVLAGLAIDGAKGVEKVREIQP
                     DVLILDIEMPNMGGLEALPKLLAAKPGLKVLMASTLTTRGANVTIRALELGAADYIPK
                     PDSTRIGGADGFRTELLTKIRALCGRTRAWPVPPAPDKPAPDKSATDKPATLERAAHA
                     HAPLGAP"
     gene            complement(576..941)
                     /locus_tag="VF402_02820"
     CDS             complement(576..941)
                     /locus_tag="VF402_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799254.1"
                     /translation="MKTCLVVDDSRVIRKVARRILENLSFEVSEANDGLEALKACKEK
                     MPSAILLDWNMPVMDGITFLRQLRKEEGGKAPVVVFCTTENDLAHITEALTEGASEYI
                     MKPFDGEILEAKFSEVGLI"
     gene            complement(967..1410)
                     /locus_tag="VF402_02825"
     CDS             complement(967..1410)
                     /locus_tag="VF402_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7799255.1"
                     /translation="MSLRDYVTLRVGGQLFGVDAARVHDVFHPRGLTPVPLSRSEIAG
                     VLNLRGRIVTAVCARRRLGLPPREDGAPEPLAIGLEVQGDSYGLVIDSVDEVLKLDDE
                     KFHAPPGNLPPRWSDIIVGVYQLEKELLIVLDPHTLLDNSHLKAA"
     gene            complement(1407..3962)
                     /locus_tag="VF402_02830"
     CDS             complement(1407..3962)
                     /locus_tag="VF402_02830"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX7799256.1"
                     /translation="MDDLIAEFLTETAESLEVVDSELVKFEANPNERKTLDNIFRLVH
                     TIKGTCGFLGLSRLEAVAHAGETLLGRFRDGKLEVTPDAVTLVLHSIDRIKVILAALE
                     ATGNEPDGDDHDLISQLEAMAEGHAVDLNAVHKVEVPDRPIGGDIEPAELNIPAVSTA
                     AAAEAAATGLQAGDVDPATGRTLRIGEVSEAELEAAFAAADGPDQENDQENSLKPGDV
                     DPATGRTLRPGEVSEADLEAAFAAAEAPDWMDEAPADVKPEPAAEKAPEPVKAAAPAP
                     RPEAAPSAPAGQSPMGDDPQGIATTQSIRVSVDVLEGLMTLVSEMVLTRNQLLQISRN
                     REDSAFATPLQRLSTLTGELQDSVMKTRMQPIGAAWKKLPRLVRDLSYELNKKIDLVM
                     EGEGTELDRQVLELIRDPLTHMVRNSADHGLEPTEERVQLGKPANGTIKLAAFHEGGY
                     IVIRISDNGRGLNTGRIREKIIEKGLASRGEVDGMSDQQIHRFIFAPGFSTAAKVTNL
                     SGRGVGMDVVRTNIEQIGGQIDLTSAPGQGTTFTIKIPLTLAIVSALIVGAGGQKFAV
                     PQTSVLELVRTGAAAEHRIEKINDSLVLRLREKLLPLVQLGPTLQLEAATDEDATFVM
                     VIQVGERRYGLVVADVLDTEEIVVKPLATILRDVDVFSGATILGDGSVVLILDPNALS
                     ERAGNMLEEKQAEDGEDAVEAGDSAKVAMLLFKAGGGAPKAVELEHITRLEHVEVEKI
                     ERMDGRAALQYRGKLMPILGLNGTQDILNEGIQPLLVFTGEGYAMGLAVDEIVDVVED
                     RLQIELSADRPGVRGTAVVSGRACEILDVDYYHLQGLAEHQRRPGASSERAVA"
     gene            complement(4185..4886)
                     /locus_tag="VF402_02835"
     CDS             complement(4185..4886)
                     /locus_tag="VF402_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEX7799257.1"
                     /translation="MSMDDLAPDLTEAREIPVAAIPVEMDAHELATQLVAKLCHDFIS
                     PAGAIISGLDLLEDPSAQDMRQEALNLISASAKKMVTLVHFARVAFGAATSAEAFNGA
                     QLQKILDDVFATMRAELAFDIPGDAVFTKPAARALLNLGLIAGNALPVGGVATLGMAR
                     DEDGLTLTAEAKGARARLKPEAVEGLKGLALGDGLSGQWIQPHWLHSVVRAANGSLDF
                     TSEPDSLSFTIRLPA"
     gene            5055..>5652
                     /locus_tag="VF402_02840"
     CDS             5055..>5652
                     /locus_tag="VF402_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7799258.1"
                     /translation="MTDTSRFARAGRGKIFDSILDTMGDTPLVRLPRLSAALGGEKGL
                     KATVLAKLEFFNPLASVKDRIGIAMIEAAEAAGRLKPGGTVIEATSGNTGIALAFVAA
                     AKGYKCILVMPESMSIERRKMLLLLGARLELTPAEKGMRGAINRAQELLAETPGGIIP
                     AQFENAANPYIHRISTAEEIWNDTNGAVDVVVSGVGTGG"
BASE COUNT          904 a         1832 c         1905 g         1011 t
ORIGIN      
        1 ggggggcgcc gagcggcgcg tgcgcgtgcg ccgcgcgctc cagcgtcgcc ggcttgtccg
       61 tggcggactt gtcgggggca ggcttgtcgg gagcgggcgg cacgggccag gcgcgcgtgc
      121 ggccgcacag ggcgcggatc ttggtcagaa gctcggtgcg gaagccgtcg gcgccgccga
      181 tgcgggtcga atcgggcttt gggatatagt ccgccgcgcc cagctccagc gcgcggatgg
      241 tgacgttggc cccgcgcgtc gtcagggtcg aggccatcag caccttcagc cccggcttgg
      301 cggccagaag cttgggcagg gcttcgagcc cgcccatgtt cggcatctcg atatcgagga
      361 tcagcacgtc cggctggatt tcgcgcacct tctcgacgcc cttggcgccg tcgatggcga
      421 ggcccgccag aaccatgtcc ggctcctgtt cgagccagcg cgagatcagg ccgcgcacga
      481 ccgccgaatc gtcgacgatc atgattttga ggggtctgag ggaagcggcg gtgtgaggat
      541 taagcgccat acgggaaagc ccggaacaga ggaaactaga taaggccgac ttccgagaac
      601 ttcgcttcca ggatttcgcc gtcgaagggc ttcatgatgt attccgacgc gccttcggtc
      661 agcgcctcgg tgatgtgcgc caggtcgttt tcggtggtgc agaacaccac gaccggcgcc
      721 ttgccgcctt cctccttgcg caactggcgc aggaaggtga tgccgtccat gaccggcatg
      781 ttccagtcga gcaggatggc cgagggcatt ttctccttgc aggctttgag ggcctccagc
      841 ccgtcattgg cctcgctgac ctcgaaagac aggttttcca ggatgcggcg cgcgacttta
      901 cggatcacgc ggctatcatc gacgacgaga caggtcttca tcctgcttgt ttcccgatac
      961 taaagattag gccgccttca gatgcgaatt gtccagcagc gtatgcgggt ccaacactat
     1021 cagaagttcc ttttccaatt gataaacgcc gacgataata tcggaccagc gcggcggcag
     1081 attgcccggc ggcgcgtgga acttttcgtc gtccagcttc agcacctcgt cgaccgaatc
     1141 gatgacgagg ccgtaggaat cgccctgcac ctcaagcccg atggccagcg gttcgggcgc
     1201 gccgtcctcg cgcggcggca agcccagacg gcggcgcgcg cacacggcgg tgacgatgcg
     1261 cccgcgcagg ttcagcacgc ccgcgatctc ggagcgcgac agcggcacgg gcgtcaggcc
     1321 gcgcggatgg aacacgtcgt ggacgcgggc ggcgtcgacg ccgaacagtt gtccgccgac
     1381 gcgtaaggtg acgtaatcac gcaggctcat gccacagccc tttcactgga ggcgcccggc
     1441 cggcgctgat gctcggccag accttgcagg tggtaataat cgacatcgag gatttcgcag
     1501 gcgcggcccg aaaccaccgc cgtgccgcgc acgccggggc ggtccgccga cagttcgatc
     1561 tgcaggcggt cctcgaccac gtcgacgatc tcgtccacgg ccagacccat cgcatagccc
     1621 tcgccggtga agaccagcag cggctggatg ccttcgttca ggatgtcctg ggttccgttc
     1681 aggcccagga tcggcatcag cttgccgcgg tattgcagcg cggcgcggcc gtccatgcgc
     1741 tcgatcttct cgacctcgac atgttcgaga cgggtgatgt gctccagctc gaccgccttg
     1801 ggcgcgccgc cgccggcctt gaacagcagc atggcgacct tggcggaatc gcccgcctcg
     1861 acggcgtcct cgccgtcctc ggcctgcttt tcctccagca tgttgcccgc gcgttcggac
     1921 agggcgttcg gatcgaggat cagcacgacc gagccgtcgc ccagaatggt ggcgccggaa
     1981 aagacgtcga cgtcgcgcag gatggtggcc agcggcttga cgacgatttc ctcggtgtcc
     2041 agcacgtcgg cgacgacgag gccgtagcgg cgctcgccga cctgaatgac catgacgaag
     2101 gtggcgtctt catccgtggc cgcctccagt tgcagggtcg gcccgagctg caccagcggc
     2161 aacagctttt cgcgcaggcg cagcaccagc gaatcgttga tcttctcgat gcggtgctcg
     2221 gcggcggcgc cggtgcgcac cagctccagc accgaggtct gcggcaccgc gaacttctgc
     2281 ccgcccgcgc cgacgatcag cgccgagacg atggccaggg tcagcggaat cttgatggtg
     2341 aaagtcgtgc cctgaccggg cgcggaggtc aggtcgatct ggccgccgat ctgctcgata
     2401 ttggtgcgca ccacgtccat gccgacgccg cgccccgaca ggttggtgac cttggcggcg
     2461 gtcgagaagc cgggcgcgaa gatgaagcgg tgaatctgct ggtcgctcat cccgtcgact
     2521 tcgccgcgcg aggccagacc cttttcgatg atcttctcgc ggatgcggcc cgtgttcagg
     2581 ccgcgcccgt tgtcggaaat gcggatgacg atgtagccgc cttcgtggaa ggccgccagc
     2641 ttgatcgtgc cgttggcggg cttgcccagt tgcacgcgct cctcggtcgg ctcaaggccg
     2701 tggtcggcgg agttgcgcac catgtgggtg agcggatcgc ggatcagctc cagcacctgc
     2761 cggtccagct cggtgccttc gccctccatc acgaggtcga tcttcttgtt cagttcgtag
     2821 gacaggtcgc gcacgaggcg cggcagcttc ttccacgccg cgccgatcgg ctgcatgcgg
     2881 gtcttcatca cgctgtcctg caattcgccg gtcagggtcg acaggcgctg caacggcgtg
     2941 gcgaaggcgg aatcctcgcg gttgcggctg atctgcaaaa gctggttgcg cgtcagcacc
     3001 atttccgaca ccagcgtcat cagcccttcc agcacgtcga ccgacacgcg gatcgactgg
     3061 gtggtggcga tgccctgcgg atcgtcgccc attggcgact gaccggcggg cgccgacgga
     3121 gcggcttccg gcctgggcgc cggggcggcg gccttcaccg gttcgggcgc cttttcggcg
     3181 gccggttcgg gcttgacgtc ggcgggggcc tcgtccatcc agtcgggcgc ctcggcggcg
     3241 gcgaaggcgg cttcgagatc ggcctcggag acttcgccgg ggcgcagggt ccggcccgtc
     3301 gccggatcga cgtcgcccgg cttcaggctg ttttcttggt cgttttcctg atcgggaccg
     3361 tcggcggcgg cgaaggcggc ttccagctcg gcctcggaga cttcgccgat gcgcagggtc
     3421 cgcccggtcg ccggatcgac atcgccggct tgcaggccgg tggcggcggc ttccgcggcg
     3481 gcggcggtcg agacggcggg gatgttcaac tcggcgggtt cgatgtcgcc gccgatcgga
     3541 cggtccggca cctcgacctt atgcacggcg ttgaggtcga cggcgtggcc ctcggccatc
     3601 gcctccagtt gcgagatcag gtcgtgatcg tcgccgtccg gctcgttgcc ggtggcttcg
     3661 agcgccgcca gaatgacctt gatgcggtcg atggagtgca gaaccagcgt caccgcgtcg
     3721 ggcgtgacct ccagcttgcc gtcgcggaag cggcccagca gggtctcgcc cgcgtgcgcc
     3781 accgcttcga ggcgcgacag ccccagaaag ccgcaggtgc ccttgatggt gtggaccagc
     3841 cggaaaatat tgtcgagcgt cttgcgctcg ttgggattgg cttcgaactt gaccagttcg
     3901 gagtcgacca cctcaagact ttcggcggtt tccgtcagga attcggcaat taaatcatcc
     3961 acagcgcaac cccgtactcg atggcttgtc ggcagaaccc tgaccgcgga cacacgtcgg
     4021 gcgggcctgt tcggggttca ctatcggagg gtttggttaa gagagggttg gcaagagatt
     4081 gacaatttct gaatttgtcg aaacggcggc gctttttcgc gggtggcgcg caaaaaaata
     4141 ccgcgccggg cggcgcggtg tttttatacg atcgggacgc gcctttaagc gggcaggcgg
     4201 atcgtgaagc tcaggctgtc cggctcggac gtgaagtcga ggctgccgtt cgccgcgcgc
     4261 acgacgctgt ggagccagtg cggctgaatc cactgcccgg acaggccgtc gcccagggcc
     4321 agccctttca ggccctcgac cgcttccggc ttgagacgcg cgcgcgcgcc tttggcctcg
     4381 gcggtcagcg tcaggccgtc ctcgtcgcgc gccatgccca gcgtggccac gcccccgacc
     4441 ggcagcgcat tgcccgcgat caggccgaga ttgagcaggg cgcgcgcggc gggcttggtg
     4501 aagaccgcgt cgcccggaat atcgaacgcc agttcggcgc gcatggtggc gaacacgtcg
     4561 tcgagaatct tttgcaattg cgcgccgttg aacgcctccg ccgaggtcgc cgcgccgaag
     4621 gcgacgcgcg cgaaatgcac cagcgtgacc atcttcttcg ccgaggccga gatcaggttc
     4681 agcgcctcct ggcgcatgtc ctgcgccgac gggtcttcca gcaggtcgag gccggaaatg
     4741 atcgcgcccg ccggggagat gaaatcgtgg cacagcttgg cgaccagttg cgtggccagt
     4801 tcgtgcgcgt ccatctcgac cgggattgcc gcaaccggga tttcgcgcgc ttccgtaaga
     4861 tcaggcgcca gatcgtccat gctcatatcg cgtcctttag ctgggctcac ctgtaatgtg
     4921 aggtgatttg ccttaaactt gaagccaaga cgtaataata tcctttaaga aggcgttaat
     4981 gcggattaag cctttacgga cgcgcgctga cagcgccggt tcaatgacaa aaacctccat
     5041 cggagacagc ccccatgaca gacacgtccc gtttcgcccg cgccggccgc ggcaagatct
     5101 tcgattcgat cctcgacacc atgggcgata cgccgctggt ccgtctgccg cgcctgtccg
     5161 cggcgctggg cggcgaaaag ggcctcaagg cgacggtgct ggccaagctg gagttcttca
     5221 atccgctggc ctcggtcaag gaccgcatcg gcatcgccat gatcgaggcc gccgaggcgg
     5281 cgggccggtt gaagcccggc gggaccgtca tcgaggcgac ctccggcaat accggcatcg
     5341 cgctggcctt cgtcgccgcc gccaaggggt acaagtgcat tctggtcatg cccgaatcga
     5401 tgtcgatcga gcgccgcaag atgctgctgc tgctgggcgc ccggcttgag ctgacccccg
     5461 ccgagaaggg gatgcgcggc gccatcaacc gcgcgcagga gctgctggcc gaaacgccgg
     5521 gcggcatcat tccggcgcag ttcgagaacg ccgccaaccc ctatatccac cgcatctcga
     5581 ctgccgagga aatctggaac gacaccaatg gcgcggtcga cgtcgtggtg tcgggcgtcg
     5641 gcaccggcgg ca
//
LOCUS       DASVLL010000170         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_332834,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000170 DASVLL010000000
VERSION     DASVLL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332834"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VF402_02845"
     CDS             <1..149
                     /locus_tag="VF402_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7799259.1"
                     /translation="SESEQFVHGFLMTTEGIELAEAFPRIRNPKFRRRILDLVRALAE
                     DGDA"
     gene            329..1504
                     /gene="metK"
                     /locus_tag="VF402_02850"
     CDS             329..1504
                     /gene="metK"
                     /locus_tag="VF402_02850"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX7799260.1"
                     /translation="MRSSYIFTSESVSEGHPDKVADRISDVVVDAFLARDPEARVACE
                     TLVTTNRIVLAGEVRAGDEEIEGIIQGLEEKVRHAVKDIGYAQKGFHWAIADYACHLH
                     AQSADIAMGVDSGHNKDEGAGDQGIMFGYATSETEELTSAPLQWSHNILRRLAEARHS
                     GQAPQLEPDAKSQVTVQYENGVPARILKILVSHQHNDGLTPKDVEAIIKPYALEVLPE
                     GMVTDETEWLVNPTGNFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVD
                     RSAAYALRYLAKNVVAAGLAKKCTLQVSYAIGVSRPLSFYVDTHGTGQVDEEKLEAIL
                     PELIGGLTPRAIRTHLGLNKPIYQRTTAYGHFGRAPDADGGFSWERTDLIEALKGAF"
     gene            complement(1542..2324)
                     /locus_tag="VF402_02855"
     CDS             complement(1542..2324)
                     /locus_tag="VF402_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7799261.1"
                     /translation="MFRSALFVPSANARAVARAPGLRCDALIFDLEDAVGAREREAAL
                     CNLRGVLSQGFAARSVLVRIHPGSLNAIHAALDGLRVDGLVAPKVNGTEDARAVMMAW
                     PKIPVWAMIETAEGVANLWEIAGMPLRGLIAGPNDLRKSLRTRPMADRADILFCLSQI
                     VVHARANGLVALDGVYNRFRDAEGFKAECEQGRALGFDGKTLIHPDQIAPCEAAFSPS
                     QAEIAWAKAVVAAFADTDAGVVAVEGEMVERLHLAQAQAILA"
     gene            2486..3241
                     /locus_tag="VF402_02860"
     CDS             2486..3241
                     /locus_tag="VF402_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX7799262.1"
                     /translation="MNDFVGQNVRARPTVEDQARDLGLKRFLLGIYQKMALGLLLTGA
                     TAWAVAHSPMFLHMLYRVTPGGQIAGYTGVGYLFLFAPLVLSFLSGAVMSRMNVAAMA
                     LFYWAFVAIMGVSLSSIFLLYTATSLASTFFITAAAFGALSLWGYTTKTNMTGWGSFL
                     IMAVFGLIIASLVNVFLLKSGMFDLVISAAGVLIFSGLLAYQTQQLKQVYYSLEQNAT
                     GLAAMTYYGALSLYINFINLFLSILRLFGGARR"
     gene            complement(3299..>3823)
                     /locus_tag="VF402_02865"
     CDS             complement(3299..>3823)
                     /locus_tag="VF402_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7799263.1"
                     /translation="MARRGQLKGGGLVATVMSNLGLERYMAAQGLALERTRVGDRYVM
                     ERMREGGFNIGGEQSGHVILSDYATTGDGLIAALQVLAERVETGRPMSELGRRFATVP
                     QLLKNVKYTGANPMTREDVQAAIKAGEERLNGSGRVLVRPSGTEPLIRVMAEGDDAAL
                     VKSVVEDIAAAMAG"
BASE COUNT          644 a         1337 c         1178 g          664 t
ORIGIN      
        1 aatcggagtc cgagcagttc gtgcacggct tcctgatgac caccgaaggc atcgagctgg
       61 ccgaagcctt cccgcgcatc cgcaatccga aattccgccg ccgcattctc gatctggtgc
      121 gcgcgctggc cgaagacggc gacgcctgag ccgtctcgcg gcgccgggcc gtttatcact
      181 gaaacctgcg cggtcacgcg ccggatgagg cctcgtcact gcatgacggg gcctttttgc
      241 atcgtaaaaa ccgcgcaaaa atcccttgat gatataaaca tatctttata tggttatggc
      301 cagcttttaa agattggaaa gtccggccat gcgttcatcc tatatcttca cctccgaaag
      361 cgtctccgaa ggccatcccg acaaggtcgc cgaccgtatc tccgacgtcg tggtcgacgc
      421 ctttctggcc cgcgatcccg aggcgcgcgt ggcgtgcgag acgctggtga cgaccaaccg
      481 catcgtgctg gccggcgagg tgcgcgcggg cgacgaggag atcgaaggca tcattcaggg
      541 cctcgaggaa aaggtgcgcc acgccgtcaa ggatatcggc tacgcgcaaa agggctttca
      601 ctgggcgatc gccgattacg cctgccacct gcacgcccag tccgccgaca tcgccatggg
      661 cgtcgattcc ggccacaaca aggacgaggg cgcgggcgat cagggcatca tgttcggcta
      721 tgcgacctcg gagaccgagg aactgacctc ggcgccgttg caatggtcgc acaacatcct
      781 gcgccgcctc gccgaggcgc gccattccgg ccaggcgccg caactggagc ccgacgccaa
      841 gagccaggtg acggtgcagt acgaaaacgg cgtcccggcg cgcatcctga aaatcctcgt
      901 ctcgcatcag cataatgacg gcctgacgcc caaggacgtc gaggcgatca tcaagcccta
      961 cgcgctcgaa gtcctgcccg agggcatggt gacggacgag acggaatggc tggtcaaccc
     1021 gaccggcaat ttcgtcatcg gcggaccgga cggcgacgca gggctgaccg ggcgcaagat
     1081 catcgtcgac acctatggcg gcgcggcccc gcacggcggc ggcgcgtttt cgggcaagga
     1141 tcccaccaag gtcgaccgct cggcggccta tgcgctgcgc tatctggcca agaacgtggt
     1201 cgcggcggga ctggcgaaga aatgcacctt gcaggtgtcc tacgccatcg gcgtgtcgcg
     1261 cccgctgagc ttctatgtcg acacccacgg cacgggtcag gtcgacgagg aaaagctgga
     1321 ggcgatcctg ccggagctga tcggcggcct gacgccgcgc gcgatccgca cccatctggg
     1381 gctcaacaag ccgatctatc agcgcaccac cgcctacggc cacttcggcc gcgcgcccga
     1441 cgccgacggc ggcttctcgt gggaacgcac cgatctgatc gaagcgctta aaggcgcgtt
     1501 ctaagccccc cccctctccc ctccatgagg ggagaggggt ttcaggccag aatcgcctgc
     1561 gcttgcgcaa gatgcaggcg ctcgaccatt tcgccctcga cggcgacgac gcccgcgtcg
     1621 gtgtccgcga aggccgcgac caccgccttc gcccacgcga tctcggcttg cgacggcgaa
     1681 aaggcggctt cgcacggcgc gatctggtcc gggtggatca gggtcttgcc gtcgaagccg
     1741 agcgcgcggc cctgttcgca ctcggccttg aacccttcgg cgtcgcggaa ccggttgtaa
     1801 acgccgtcca gcgcgacaag cccgttggcg cgcgcatgca ccacgatctg cgacaggcag
     1861 aacagaatgt cggcgcgatc cgccattggc cgcgtgcgca aggatttgcg cagatcgttc
     1921 ggcccggcga tcaggccgcg caacggcatc ccggcgatct cccacagatt ggcgacgccc
     1981 tcggcggtct cgatcatcgc ccacaccgga atcttcggcc acgccatcat caccgcgcgc
     2041 gcatcctcgg tcccgttgac cttgggcgcg accagcccgt cgacgcgcag gccgtccagc
     2101 gccgcatgga tggcgttcag actgcccgga tggatgcgca ccagcaccga ccgcgccgcg
     2161 aacccctgag acaaaacgcc gcgcaggttg caaagcgccg cctcgcgctc ccgcgccccg
     2221 accgcgtcct ccagatcgaa gatcagcgcg tcgcagcgca ggcccggcgc cctggcgacg
     2281 gcgcgggcgt tggcggacgg aacgaacagc gcggaacgga acatggcgga tctcctcggc
     2341 cccgctatag cagaccggcg ggaagcggcg caaatcccct tgccgcactc tccctcttgc
     2401 cacacctttg ccgcaggccc atattagcct catcgaacgg tgacggcgtt ttcctcgtcg
     2461 tcacgtctat atcaggggtt ttccaatgaa cgactttgtc ggtcaaaatg tgcgcgcccg
     2521 gcctacggtt gaggatcagg cccgcgatct gggcctgaag cgcttcctgc tcggcatcta
     2581 tcagaagatg gcgctggggc tgctgctgac cggcgcgacg gcctgggcgg tcgcccattc
     2641 gccgatgttc ctgcacatgc tctatcgcgt gacaccgggc gggcagatcg ccggttacac
     2701 cggggtcggc tacctgttcc tgttcgcgcc gctggtgctg tccttcctgt cgggcgcggt
     2761 gatgtcgcgc atgaacgtgg cggcgatggc gctgttctac tgggccttcg tggcgatcat
     2821 gggcgtgtcg ctgtcgtcga tcttcctgct ctacaccgcg acctcgctgg cctcgacctt
     2881 cttcatcacc gcggcggcct tcggcgccct gtcgttgtgg ggctatacga ccaagaccaa
     2941 catgaccggc tggggctcgt tcctcatcat ggccgtgttc gggctgatca tcgcctcgct
     3001 ggtcaatgtc ttcctgctca agagcgggat gttcgacctc gtcatctcgg cggcgggcgt
     3061 gctgatcttc tccggcctgc tggcctatca gacccagcaa ctcaagcagg tctattactc
     3121 gctggagcaa aacgcgaccg gcctcgcggc catgacctat tacggcgcgc tgtcgctcta
     3181 catcaacttc atcaacctgt tcctgagcat cctgcgcctg ttcggcggcg ctcgccgctg
     3241 atcgcgggac ggctgaaaaa gaaaaagccc tccggcgcga gccggggggc ttttttcatt
     3301 acccggccat cgccgccgcg atgtcctcga ccaccgactt gaccagcgcc gcgtcgtcgc
     3361 cctcggccat gacgcggatc agcggctcgg tccccgaagg ccgcaccagc acccggcccg
     3421 acccgttcag ccgttcctcg cccgccttga tcgccgcctg cacgtcctcg cgcgtcatcg
     3481 gattggcgcc ggtatatttc acgtttttca gaagctgcgg cacggtggcg aaccgccgcc
     3541 ccagctcgct catcggcctg cccgtctcga cgcgctcggc cagcacctgc aaggccgcga
     3601 tcagcccgtc gccggtggtg gcgtagtcgc tcaggatcac atgccccgac tgctcgccgc
     3661 cgatattgaa gccgccctcg cgcatccgct ccatcacgta gcggtcgccg accctggtgc
     3721 gctccagcgc gaggccctga gcggccatgt agcgttcgag gccgagattg gacatgacgg
     3781 tggccaccag cccgccgccc ttgagctggc cccggcgcgc cat
//
LOCUS       DASVLL010000171         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_10857, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000171 DASVLL010000000
VERSION     DASVLL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10857"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VF402_02870"
     CDS             <1..1339
                     /locus_tag="VF402_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7799264.1"
                     /translation="SGWAFDRSAYFEGALREAVNNGYVPQARLDDMARRILYAMFATG
                     AVDDQVKGDQSATIDYAAHAKVTQADAEAGIVLLKNTKGLLPIAKTAKKIVVIGGHAD
                     FGVLSGGGSSQVYTPGWKRFDGEGPDGFPGPMTYYPSSPVKGIEARSSASVTYLDGKD
                     VKAAAKAARGADLVIVFGTQWTGESVDVPDLNLPDNQDALISAVAKANKKTVVVLETG
                     GPVVMPWLNSVGAVLEAWYPGASGGEAIARVLTGEVNPSGHLPATFPAGVDQLPRTVL
                     DGDPKLDENSHPHTDYDIEGAAVGYKWFDKKGYKPLFPFGYGLSYTSFQLGGLTAQAD
                     GKGLKASFSIRNTGASDGKEVAQIYVAPAADAGWEAPKRLGGFVKADLKPGESRTLTA
                     SVDPRLLGTYDSASKTWTIKAGDYKVMLATSAADIAQTVTVHLDQQTLNVKGQ"
     gene            1549..2343
                     /locus_tag="VF402_02875"
     CDS             1549..2343
                     /locus_tag="VF402_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX7799265.1"
                     /translation="MNLRLILALFAASFAAFGAAAQDYQSSPPRQDATPRDFGPYLGP
                     YRARVTAKMLEDFGEQYLYAPLNAVLPAPKPGEDRVVFLGDSITDRWNLAKFFPGRPY
                     VNRGIGGQITTQMIVRFHADVAALHPKAVVILAGVNDVQGVFQVESEQQIETNYLTLA
                     ELAEANHIVPIFTKITPLNNYTPNAGTVLQDRHADELARLNTWLEGFCAGRGYTLIDY
                     GPTLRDAKGLMRADYTTDGIHPTDSAYAVMAPVAEKAIESALAVKR"
BASE COUNT          460 a          854 c          782 g          398 t
ORIGIN      
        1 atcgggctgg gccttcgacc gctcggccta tttcgagggc gcgctgcgcg aggcggtcaa
       61 taacggctat gtgccgcagg cgcgcctcga cgacatggcc cgccgcatcc tctacgccat
      121 gttcgccacc ggcgcggtcg acgatcaggt caagggcgac cagtcggcca cgatcgacta
      181 cgccgcccac gccaaggtga cgcaggccga cgccgaggcc ggcatcgtgc tgctcaagaa
      241 caccaaaggt ctgctgccca tcgccaaaac cgcgaaaaag atcgtggtga tcggcggcca
      301 cgccgatttc ggcgtgctgt cgggcggcgg ctcgtcgcag gtctatacgc cgggctggaa
      361 acgctttgac ggcgaaggcc cggacggctt ccccggaccg atgacctatt atccgtcctc
      421 gccggtgaag gggatcgagg cgcgctcttc ggcgtccgtg acctatctcg acggcaagga
      481 cgtcaaggcg gcggcgaagg ccgcgcgcgg ggccgacctg gtgatcgtgt tcggtacgca
      541 atggaccggc gaaagcgtcg acgttcccga cctgaacctg cccgacaacc aggacgcgct
      601 gatctcggcg gtggcgaagg ccaacaagaa gaccgtggtg gtgctggaaa ccggcggccc
      661 ggtcgtcatg ccgtggctga acagcgtcgg cgccgtgctg gaggcatggt atcccggcgc
      721 cagcggcggc gaggccatcg cgcgcgtgct gaccggcgag gtcaatccgt ccggccatct
      781 gcccgccacc ttcccggctg gcgtcgatca gttgccgcgc accgtactgg acggcgatcc
      841 caagctggac gagaattcgc atccgcacac cgattacgac atcgaaggcg cggcggtcgg
      901 ctacaagtgg ttcgacaaga agggctataa gcctttgttc ccgttcggct acggcctcag
      961 ctacaccagc ttccagttgg gcggccttac ggcgcaagcg gacggcaagg gcctgaaggc
     1021 gtcgttcagc atcaggaaca ccggcgccag cgacggcaag gaggtcgcgc agatctacgt
     1081 cgcgcccgcc gccgacgcgg gctgggaagc gcctaagcgc ctcggcggct tcgtcaaggc
     1141 cgacctgaaa cccggcgaga gccggaccct gaccgccagc gtcgatccgc gcctgctcgg
     1201 aacctacgat tccgccagca agacctggac gatcaaggcc ggcgattaca aggtgatgct
     1261 ggcgacctcg gccgccgaca ttgcacaaac ggtcaccgtc catctcgacc agcaaaccct
     1321 gaacgtcaag gggcaataag cgcctctgtc acccctctcc cctccacgag gggagaggac
     1381 gaggagcaag gcgcagccgc cgatcctcgg gtgaggggcc gccagacatc tgagcgtaac
     1441 aggcgtctga gcgtaacgtc gaacaggccc ctcacccgat cgccttcgct acgctcggct
     1501 ctcgtcctct ccccgcaagc ggggagaggg atagttatga gacttcccat gaatctccgc
     1561 ctgatcctcg ccctattcgc cgcgtccttc gcggctttcg gcgccgcggc acaggactat
     1621 cagtcctcgc cgccgcgtca ggacgcgacg ccgcgcgatt tcggcccgta tcttggcccc
     1681 taccgcgcca gggtcaccgc caagatgctg gaggatttcg gcgaacaata tctctacgcg
     1741 cccttgaacg ccgtcctgcc cgcgccgaaa cccggcgagg atcgcgtggt gtttctgggc
     1801 gattcgatca ccgaccgctg gaatctggcg aaattctttc ccggcaggcc ctatgtcaat
     1861 cgcggcatag gcggccagat caccacccag atgatcgtgc gcttccacgc cgacgtcgcc
     1921 gccctgcatc cgaaggccgt ggtcattctg gcgggcgtca acgacgtgca gggcgtgttt
     1981 caggtcgaga gcgaacagca gatcgaaacc aattacctga ccctggccga gctggccgag
     2041 gccaatcaca tcgtcccgat cttcaccaag atcacgccgc tcaacaatta cacgcccaac
     2101 gccggaaccg tgctgcaaga ccgccacgcc gacgagttgg cgcggctcaa cacctggctg
     2161 gaagggttct gcgccgggcg gggctatacg ctgatcgact acggcccgac tttacgcgac
     2221 gccaagggcc tgatgcgcgc cgactacacc accgacggca ttcatccgac cgattcggcc
     2281 tatgcggtca tggcgccggt ggcggaaaag gccatcgaat cggcgctggc ggttaagcgc
     2341 taagcagctt ctccagccgc tccgccgcgt cggagatcgc gaaggccgcc ttgcgctcca
     2401 tctcgtgcgc gcccgcattg atgcgcgcat tggccgaatg cgcctcggcc agttgcgcgc
     2461 gcagatcctg catctcgtcg atcatcaaaa gcgc
//
LOCUS       DASVLL010000172         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_385858,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000172 DASVLL010000000
VERSION     DASVLL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385858"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /gene="proB"
                     /locus_tag="VF402_02880"
     CDS             <1..912
                     /gene="proB"
                     /locus_tag="VF402_02880"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX7799266.1"
                     /translation="KRARLKLDEKQAAAAAGQPRLMSAWEAAFAAVGLKSAQVLLTRE
                     DTEKRRRWLNARATVDTLLALGCVPVVNENDTLATEEIRYGDNDRLAARTAQLVRAET
                     LLLLSDIDGLYTDDPRRNPEAEHIAVVEKLDARIEAMAGGANLDAGVGTGGMATKIAA
                     ARIAGSAGCATLIAFGDVARPLSQDRRFTLIRPPSTTAAAYKAWIAGTVVPAGHLTLD
                     EGAVRALKNGKSLLPSGIVEVAGAFEKGDSVAILTPAGAEIARGVVTYSADAIRQIRG
                     RASKDIEAILGYTSGDEVIHRDDMVLM"
     gene            952..1371
                     /locus_tag="VF402_02885"
     CDS             952..1371
                     /locus_tag="VF402_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX7799267.1"
                     /translation="MRLVLDTSTIVAAMRSPAGVSAHLLRRARLKQISLLANIALALE
                     YEAVCLRPEHLTAAQLTAAEANIFVTAVIALCEPVESYFLWRPQLRDPADEFVLEAAV
                     NGRAEAIITFNRRDFLPAAQRFGIEIVRPDEILRRLS"
     gene            1368..>1609
                     /locus_tag="VF402_02890"
     CDS             1368..>1609
                     /locus_tag="VF402_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799268.1"
                     /translation="MNEARAKRPTQNYALRLPASLKAEVERVAHDEGASINQFIATAV
                     AEKLAALKTAAYFAEKRALADFEAFDRIMARSGGQPP"
BASE COUNT          281 a          516 c          554 g          258 t
ORIGIN      
        1 aagcgggcgc ggctcaagct ggatgaaaag caggccgccg ccgccgccgg gcagccccgg
       61 ctgatgagcg cgtgggaagc cgccttcgcc gccgtcgggc tgaaaagcgc gcaggtgctg
      121 ctgacgcgcg aggacaccga aaagcgccgc cgctggctca atgcgcgcgc cacggtggac
      181 acgctgctgg cgctgggctg cgtgccggtg gtcaacgaga acgacacgct ggcgaccgag
      241 gaaatccgct acggcgacaa cgacagatta gcggcgcgca cggcgcaact ggtgcgcgcc
      301 gagacgctgc tgctgctgtc ggacatagac ggtctgtaca cggacgatcc gcgccgcaac
      361 ccggaggccg agcatatcgc cgtggtcgaa aagctcgacg cgcgcatcga ggcgatggcg
      421 ggcggcgcca atctcgacgc cggggtcggc acgggcggca tggcgaccaa gatcgccgcc
      481 gcgcgcatcg ccggttcggc gggctgcgcg acgctgatcg cgttcggcga cgtggcgcgg
      541 cccctgtcgc aggaccggcg cttcaccctg atccgcccgc catcgaccac ggcggcggcc
      601 tataaggcgt ggatcgcggg cacggtggtc ccggcgggtc atctgacgtt ggacgaaggc
      661 gcggtgcggg cgctgaaaaa cggcaagtcg ctgctgccgt ccggcattgt cgaggtcgcg
      721 ggcgcgttcg agaagggcga cagcgtcgcc atcctgaccc cggcgggcgc ggaaatcgcg
      781 cgcggcgtcg tcacctactc cgccgacgcc atccgccaga tcagaggccg cgcctcaaag
      841 gacatcgagg cgattttagg ctataccagc ggcgacgagg tcatccatcg cgacgatatg
      901 gtgctgatgt agtctatttt catatcgcat gcgatatgat atgaaggcgc tatgcggctc
      961 gttctcgaca ccagcacgat tgtcgccgcc atgcgcagcc cggcgggcgt ttcggcgcat
     1021 ctgctgcgtc gcgcgcgtct gaaacagata agcctgctgg ccaatatcgc gttggcgctg
     1081 gagtacgagg cggtctgtct gcgtcctgag catctgacgg cggcacaatt gacggcagcg
     1141 gaggcgaata tctttgtcac cgccgtcatc gctctgtgcg agccggtcga gtcgtacttt
     1201 ctgtggcgtc cgcaattgcg cgatccggcg gacgagtttg ttctcgaagc cgccgtcaat
     1261 ggccgcgccg aggccattat caccttcaac cggcgcgatt tcctgcccgc tgcgcaacgc
     1321 ttcggcatcg agattgtcag gccggacgag attttgagga gattgtcatg aacgaagcca
     1381 gagccaagcg tccgacgcag aactatgcgc tgcgtctgcc ggcgtccctg aaggccgagg
     1441 tcgaacgggt cgcgcacgat gaaggcgcca gcatcaacca gttcatcgcc accgccgtgg
     1501 ccgagaagct ggcggcgctc aagacggcgg cctattttgc tgaaaagcgc gcgctggccg
     1561 atttcgaggc gttcgaccgc atcatggcgc gctccggcgg ccagccgcc
//
LOCUS       DASVLL010000173         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_63706, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000173 DASVLL010000000
VERSION     DASVLL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63706"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..2331)
                     /locus_tag="VF402_02895"
     CDS             complement(106..2331)
                     /locus_tag="VF402_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7799269.1"
                     /translation="MKAFLTVSVCALALTGAAHAQTRAWMDRTQSAEARARAAVQAMT
                     EDEKLSLVMGYTDPKTLAKQSDADVSPALKADVAAHAIDGAAGYVPGVPRLGIPPQWQ
                     TDASIGVRVEGMERTALPSSLATAASFDPAVPYAGGVMIAHEARLSGFNTFLAGGANL
                     AREPRNGRNFEYAGEDPWLAGQMAGSLIQGIQSTHMVSTMKHFAVNDQESQRTTVDVT
                     ISPEAMRQSDLLAFEFVYEKGHPGSVMCAYNLVGGRWTCENEYLLQKTLKHDWGFKGY
                     VMSDWGAVHSTADAVNGGLDQMTGWPCCGDKVPYFNAPRLKAALADGQITQQRLDDMA
                     TRVLWPLFEDGVIDDPVKVGPIDFKADAAVSQDAAEQSLTLLKNNDDLLPLSGVKTVA
                     VIGGHADKGVLSGGGSSGVHPVGGSALPVEKGWPGSAIWMPSAPLDALKAELPDAKIA
                     YASGDDIAAAATAAAKAEVAVVFVTDWKGEGFDTPLELKDNQDALIAAVVKANPKTVV
                     VLETGGAVLMPWADQVPAILEAWYPGIRGGAAIARVLSGKVDPSGHTPISFPASNAQL
                     AHADIPGFDQPGDTPVHITYDEGAAIGYKWYDVKGYTPLFAFGHGLSYTTFAASDARA
                     EVSDDAITVTASLANTGHKDGKGVLMAFVAPADWKAAGWEAPKRLAGFAKAGLTPGAR
                     QTFTFRIDPRLLATWEAADDSWRIKAGTYRILLGQASDDLPLSLDVTLSGKTWSAVHA
                     Q"
     gene            complement(2404..2700)
                     /locus_tag="VF402_02900"
     CDS             complement(2404..2700)
                     /locus_tag="VF402_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7799270.1"
                     /translation="MAYRVDLTARAVRDLRHIFETIDAQNSTAAAEWFSGLEAAVLAL
                     ENQPHRHPAAPEKDGLRHILHPSRSYVYRVIFKIDDARRIVSVLHIRHGSRRAL"
BASE COUNT          438 a          891 c          990 g          487 t
ORIGIN      
        1 ttgcgtttat gacgatctac aggccggttc ctccggcgcg aagattagcg cggatcggca
       61 aggcggcaat cggaatacgt attcacagag aggacgacgc ggatgctact gcgcgtggac
      121 ggcgctccag gtcttgccgc tgagggtcac gtcgaggctg agcggcaggt cgtcggacgc
      181 ctggccgagc aggatgcgat aggttccggc cttgatgcgc caactgtcgt cggcggcttc
      241 ccaggtggcc agcaggcgcg ggtcgatcct gaaggtgaag gtctggcggg cgccgggggt
      301 caggccggcc ttggcgaaac cggcgaggcg tttgggcgcc tcccatccgg cggccttcca
      361 gtcggcgggg gcgacgaagg ccatgagcac gcccttgccg tctttatggc cggtgttggc
      421 gaggctggcg gtgacggtga tcgcgtcgtc gctgacctcg gccctggcgt ccgacgcggc
      481 gaaggtcgtg tagctcaggc cgtggccgaa ggcgaacagc ggcgtgtaac ccttcacatc
      541 gtaccatttg tagccgatgg ccgcgccttc gtcataggtg atgtggacgg gcgtgtcgcc
      601 gggctgatcg aagccgggaa tgtcggcatg ggccagttgc gcgttcgagg ccgggaagct
      661 gatcggcgtg tggccggacg gatcgacctt gccgctcagc acgcgcgcga tggccgcgcc
      721 gccgcgaatg cccggatacc aggcttcgag aatcgccgga acctgatcgg cccacggcat
      781 cagcacggcg ccgccggttt ccagcaccac gaccgtcttc ggattggcct tgaccacggc
      841 ggcgatcagc gcgtcctgat tgtccttcag ctccagcggg gtgtcgaagc cttcgccctt
      901 ccagtcggtg acgaacacca cggcgacctc ggctttcgcg gcggcggttg cggcggcggc
      961 gatgtcgtcg ccggaggcgt aggcgatctt cgcgtcgggc agttcggcct tcagcgcgtc
     1021 gagcggcgcg gacggcatcc agatcgccga accgggccag cccttttcca ccggcagggc
     1081 gctgccgccg accggatgca cgccggacga gccgccgccg gacagcacgc ccttgtcggc
     1141 atggccgccg atcacggcga cggttttcac gccggaaagc ggcaagaggt cgtcattatt
     1201 cttaagcagg gtcagcgact gctcggcggc gtcttgcgac acggcggcgt cggccttgaa
     1261 gtcgatcggg ccgaccttca ccggatcgtc gatcacgccg tcttcgaaca agggccacag
     1321 gacgcgcgtc gccatgtcgt cgaggcgctg ctgcgtgatc tggccatcgg ccagcgcggc
     1381 cttgaggcgc ggcgcgttga aatacggaac cttgtcgccg cagcacggcc agccggtcat
     1441 ctggtcgagg ccgccattga ccgcgtcggc ggtcgaatgc accgcgcccc agtcggacat
     1501 gacatagccc ttgaagcccc agtcgtgctt gagcgtcttt tgcagcaggt attcgttctc
     1561 gcacgtccag cggccgccga cgagattgta cgcacacatg accgagccgg gatggccctt
     1621 ttcatagacg aattcgaagg ccagcaggtc ggactggcgc atggcttcgg gcgagatcgt
     1681 gacgtcgacc gtggtgcgct ggctttcctg atcgttgacg gcgaagtgct tcatggtcga
     1741 gaccatgtgc gtcgactgga tgccctgtat caacgatccg gccatctggc ccgccagcca
     1801 cggatcttcg ccggcatatt cgaaattgcg cccgttgcgc ggctcgcgcg cgagattggc
     1861 gccgccggcc aggaaggtgt tgaagccgga taacctggcc tcatgcgcga tcatcacgcc
     1921 gcccgcatag ggcacggcgg ggtcgaacga ggcggcggtg gccaaagacg agggcagggc
     1981 ggtgcgctcc atgccttcga cgcgcacgcc gatcgaggcg tcggtctgcc actgcggcgg
     2041 gatgccgagg cgcggcacgc cggggacata gcccgccgcg ccgtcgatgg cgtgggcggc
     2101 gacatcggcc ttcagcgcgg gcgagacgtc ggcgtccgac tgtttggcga gggttttcgg
     2161 gtcggtatag cccatgacga ggctgagctt ttcgtcctcg gtcatggcct gtaccgccgc
     2221 cctggcgcgc gcctcggcgg actgcgtcct gtccatccag gcccgcgtct gcgcatgggc
     2281 ggcgccggtc agcgccagcg cgcacacgct tacggttaga aacgctttca tcgaacggcc
     2341 tccgctgtct tatcgttagg gcgcaggcta taaggaaagc gctgtcaggg gaagggcaaa
     2401 ccgtcacaag gcccggcgcg atccgtgacg gatatggagc acgcttacga tccgccgcgc
     2461 gtcgtcgatc ttgaaaatga cgcgatagac gtaagagcgg ctggggtgca ggatatggcg
     2521 caggccgtcc ttttccggcg cggcgggatg gcggtgaggc tggttttcga gcgcgagaac
     2581 ggctgcttcc agtccgctga accattcggc cgcggcggtc gagttctgcg cgtcgatcgt
     2641 ctcgaaaata tgcctgaggt cgcggacggc gcgcgcggtc aggtcaacgc gataggccat
     2701 agtcgcggcg cagctcatcg aaggcgtccg aagccccccg gacgcgaccc gcctcaagat
     2761 cttcgaggcc ctgccgcacg ccttccccgg cgtcggcctg cgcggc
//
LOCUS       DASVLL010000174         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_385905,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000174 DASVLL010000000
VERSION     DASVLL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385905"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1589)
                     /locus_tag="VF402_02905"
     CDS             complement(<1..>1589)
                     /locus_tag="VF402_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7799271.1"
                     /translation="RHGRGADIVVADVEKANPNALFEYDLGNAPLGEVPSSWIGYDPT
                     LRYYNGLMWYQKRFDAGPELIRKDGRVFLHFDAADYHAMIYLNGHKVGEHFGGFTPFT
                     FEVTDIIRAKDNNLVVGVDSTRTADTIPPTVTDWETYGGITRDVKLISTPATYIDDAF
                     IRLTRDGQIKADMALDGAGKAGQAVEIAVAGTPLVLKGTTDAQGRVSMSAAAPKGLKL
                     WSPDTPTLYNVTFRAGADAITDQIGFRTIEVQGAKILLNGKPIFLRGVAMHEEEFGPN
                     PSRRMTEEASRALLTEIKDGLHGNYVRLAHYPHNEITTRMADKLGLLVWSEIPVYWSV
                     NFSSPDTLKTAQAMLAENILRDRNRAAIALWSVGNETPVSDARDHFMGTLAQEARDLD
                     GTRLVTAALNTATVNGPTDISIKDPLAADLDVIGINTYNGWYGNNTLDDVKKTVWHSD
                     YNKPMIFSEFGAGARFGYRDPEQQHRFSEDYQASYYRATLAMADNIPFLAGMSPWILK
                     DFRSPRRQNIYQQGWNRKGLV"
BASE COUNT          249 a          509 c          518 g          313 t
ORIGIN      
        1 caccaggccc ttgcggttcc agccctgctg atagatgttc tgacggcgtg gcgagcggaa
       61 gtccttgaga atccacggcg acatcccggc caggaacgga atgttgtccg ccatggccag
      121 cgtcgccctg taatagctgg cctgatagtc ctcgctgaaa cggtgctgct gctccgggtc
      181 gcgatagccg aacctggcgc ccgcgccgaa ctccgagaag atcatcggct tgttgtagtc
      241 ggaatgccag acggtcttct tcacgtcgtc cagcgtgttg ttcccgtacc agccgttata
      301 ggtgttgatg ccgatgacgt cgagatcggc ggccagcggg tctttgatcg aaatatcggt
      361 cgggccgttg accgtggccg tgttcagcgc cgccgtcacc agccgcgtgc cgtccagatc
      421 gcgcgcctcc tgcgccagcg tccccatgaa gtggtcgcgc gcgtccgaca ccggcgtctc
      481 gttgccgacg ctccacagcg cgatcgccgc ccggttgcgg tcgcgcagga tgttctcggc
      541 cagcatggcc tgcgcggttt tcagcgtgtc ggggctggag aagttcacgc tccaataaac
      601 cgggatttcg ctccacacca gaagccccag cttgtcggcc atgcgcgtgg tgatctcgtt
      661 gtgcggatag tgggccagcc gcacatagtt gccgtgcagc ccgtccttga tctcggtcag
      721 cagcgcgcgc gaagcttcct cggtcatgcg gcgcgacgga ttggggccga actcctcctc
      781 gtgcatggcg acgccgcgca ggaagatcgg cttgccgttg agcaggattt tcgcgccctg
      841 aacctcgatg gtgcgaaagc cgatctggtc cgtgatcgcg tccgcgcccg ccctgaaggt
      901 cacgttgtac agagtcggcg tgtccggcga ccacagtttc agccccttcg gcgccgccgc
      961 gctcatcgag acgcggcctt gcgcgtcggt ggtccctttc aggaccagcg gcgttccggc
     1021 gaccgcgatc tcgaccgcct gaccggcctt gcccgcgccg tccagcgcca tgtccgcctt
     1081 gatctggccg tccctggtca ggcggatgaa ggcgtcgtcg atataggtgg cgggcgtgga
     1141 aatcagcttc acgtcgcggg tgatgccgcc ataggtctcc caatcggtga cggtcggcgg
     1201 aatcgtgtcg gcggtgcgcg tcgaatcgac cccgacgacg aggttgttgt ccttggcgcg
     1261 gataatgtcc gtgacctcga aggtgaacgg ggtgaagccg ccgaaatgct cgcccacctt
     1321 atggccgttg agatagatca tggcgtgata gtcggcggcg tcgaaatgca ggaagacgcg
     1381 cccgtccttg cggatcagct ccggcccggc gtcgaagcgc ttttggtacc acatcagacc
     1441 gttgtaatag cgcaaggtgg gatcgtagcc gatccacgac gacggcacct cgcccagcgg
     1501 cgcgttgccg agatcgtact cgaacagggc gttgggattg gccttctcca cgtccgcgac
     1561 cacgatgtcg gcgccgcgtc cgtggcgca
//
LOCUS       DASVLL010000175         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_224858,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000175 DASVLL010000000
VERSION     DASVLL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224858"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VF402_02910"
     CDS             complement(<1..694)
                     /locus_tag="VF402_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799272.1"
                     /translation="MSKLRPSLIPLVLLCLAPAARAQDTSVTVTREALPISKIGQSVD
                     VLTADDIQSYQSVFVSDLLLHTTDLSIARNGGPGEAAAASIRGAGADHTLYILNGIRL
                     NDPSQVGGGTNLGLLNTGDAARIEVLRGPLSTLWGPGALGGVVSVVTRTAQAPLEGDL
                     NLEGFDHYGAARLGFGGKDDRLNWRFSTGLINDQGVSAFAGGTEKDGFSQGDASLTAG
                     YAFSDDLKLNGLY"
     regulatory      complement(802..991)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1068..2384)
                     /locus_tag="VF402_02915"
     CDS             complement(1068..2384)
                     /locus_tag="VF402_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7799273.1"
                     /translation="MFNHLRRFPRARAGNAVLIFALVMPVLLMAIGGAIDFGRAVQLR
                     VAMQDAADVASLGAVAINSAAYKVALQMGDGEITAGTQQAHDIYVSDMISAPELSTPI
                     ITTQVSKSGTVLTSNVSVSANYKPYLLQLFGFATLPLSVTSKSSSTIPPYIDFYLLLD
                     NSPSMGLGATTADINKLVSATSKMSSDANCAFACHLSGSTTDYYSLARKVGVTMRIDV
                     VREATQNLMTTAKNTQTLNGQYRMAIYDFGVAADKISQTAPPAYQVSALTSNLTQSAS
                     DAGTIDLMTIPAQNYNSDRQSNFSSVLASMNTAIPTPGNGTSATNPQKVLFMVSDGLN
                     DGYDCAYGSCRRITPLDISQCTAMKARGVRIAVLYTTYLLLDKGQNSFFDGNVRKYVT
                     PTDQIATNMQACASPGLYFAVGPDEGISEAMTALFNKVVQVVRINS"
BASE COUNT          445 a          742 c          825 g          472 t
ORIGIN      
        1 tgtaaaggcc gttcagcttc aggtcgtcgc tgaacgcgta tccggcggtc aggctggcgt
       61 cgccttgcga aaagccgtcc ttttccgtgc cgcccgcgaa ggccgacacg ccctgatcat
      121 tgatcaggcc cgtgctgaaa cgccagttca ggcggtcgtc cttgccgccg aagccgaggc
      181 gggccgcgcc gtaatggtcg aagccctcaa ggttgagatc gccttccagc ggcgcctgcg
      241 cggtccgggt gacgacgctg accacgccgc ccagcgcgcc gggcccccac agggtcgaaa
      301 gcggcccgcg cagcacctcg atgcgcgcgg cgtcgccggt gttcagcagg ccgagattgg
      361 tcccgccgcc gacctgcgac ggatcgttca ggcgaatgcc gttcaggatg tacagcgtat
      421 ggtccgcccc ggcgccgcgg atcgaggcgg cggcggcttc gcccggaccg ccgttgcgcg
      481 cgatgctgag atcggtggtg tgcagcaaca ggtcggacac gaagacggac tgataggact
      541 ggatatcgtc ggcggtcagc acgtcgacgc tctggccgat cttcgagatc ggcagggcct
      601 cgcgcgtgac ggtgacgctg gtgtcctgcg cgcgcgcggc gggcgccagg cacaacagga
      661 cgagcggaat cagggaagga cgcagtttgg acacgggttt cgctccacag gacggcgaaa
      721 gccgtatgcg gcggcatacg gctgacaaca catcgtcgct caggaaacaa agacctgctc
      781 gccccggctg gacccggccg gacaagggac gacagccaag gcaggtctcc tgacttgcgc
      841 ctcgaacgcc gggcggcgcc ttcccccgtt aacggagtga cgtttgccgt ccggctcggc
      901 gcttacagtt gcgggcacag tccgggattt tcacccggtt ccctattctc ccacggtcgt
      961 gaccgcaggc tccttggcac ggcgcgggcg atagggcttt cgcccccgcc ggtcaagcga
     1021 aaaccgggcc gggacggcgg aaaggcgcgc cctgttgcgc gattgcgcta gctgttgatg
     1081 cgcaccacct gcacgacctt gttgaacaag gcggtcatgg cctcgctgat gccttcgtca
     1141 gggccgacag cgaaatacag gcccggcgag gcgcacgctt gcatattggt cgcaatctgg
     1201 tcggtcggcg tgacatactt gcgcacatta ccgtcaaaga agctgttttg ccctttatct
     1261 agaagtagat aggtcgtgta tagcaccgcg atgcggacgc cgcgcgcctt catggctgta
     1321 cattggctga tgtcgagtgg agtaatgcgg cggcaggagc cgtaagcgca gtcgtaaccg
     1381 tcattcagtc catccgatac catgaacagg actttctgtg ggttggtggc tgacgttcca
     1441 ttgccgggcg tagggatggc cgtattcatg ctcgccaaga ctgagctgaa attgctctgc
     1501 cgatccgaat tataattctg ggcggggatc gtcatcaggt cgatcgtgcc ggcgtcactg
     1561 gccgattgcg tcaggttgct ggtcagcgcg ctgacctgat atgctggggg cgccgtctgg
     1621 ctgatcttat ctgcggccac gccgaaatcg taaatcgcca tgcgatactg accgttgagc
     1681 gtttgcgtgt ttttcgctgt cgtcatcaga ttttgcgtgg cctcacgcac cacgtctatg
     1741 cgcatggtca cgccgacttt tctggccaga gaataatagt ccgtggtcga accgcttaga
     1801 tggcaggcga aagcgcagtt ggcatcactg gacatttttg aggtggcgga cacaagcttg
     1861 ttgatatccg ccgtcgtcgc ccccagtccc atcgacggtg aattatccag cagcaggtag
     1921 aagtcgatat agggcgggat ggtcgaggac gatttcgacg tgacgctgag cggcagggtg
     1981 gcgaagccga acagttgcag caggtagggc ttgtaattgg cgctgacgct gacgttcgag
     2041 gtcagcaccg tgccggactt gctgacctgg gtggtgatga tcggggtcga cagctccggc
     2101 gcgctgatca tgtcggacac gtagatgtcg tgcgcctgct gcgtcccggc ggtgatctcg
     2161 ccgtcgccca tttgcagcgc gaccttgtag gcggcggaat tgatcgccac ggcgccgagg
     2221 ctggcgacat cggcggcgtc ctgcatcgcc acgcgcagtt gcaccgcccg gccgaagtcg
     2281 atggcgccgc cgatggccat caagaggacc ggcatgacca gtgcaaagat cagaaccgca
     2341 ttgcccgcgc gggcgcgcgg aaaacggcgc aagtggttga acacgtcagg cccccgacat
     2401 tctgtagagt cggggcagta tagggcgaca ggattaagcc ttgcctaatc cgctgaattc
     2461 ttccggcgtc agcgcgcgca ggga
//
LOCUS       DASVLL010000176         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_224865,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000176 DASVLL010000000
VERSION     DASVLL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224865"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1657
                     /gene="thrC"
                     /locus_tag="VF402_02920"
     CDS             257..1657
                     /gene="thrC"
                     /locus_tag="VF402_02920"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7799274.1"
                     /translation="MNYIGTRGFSEPRSFAGALLDGLAPDGGLYVPQTYPQPGFDLAA
                     AAAGDYGALTEKLLTLFGVDVLGAEAVTAAAARTKAAFLRMTPSGGQTPLTQLDDNLW
                     LLELYHGPTLAFKDMAMQMIAPLTDAALAQTGEKLTLVTATSGDTGAAAVRAFAGSAH
                     VRLVVFHPLGRVSPVQRLQMTTVDADNVLNVGVEGDFDDCQRIVKRLLGEASLKERGL
                     ISSVNSINWGRLLGQVPYYLAASAASGADRPDFVAPTGNFGDAFAGWVARRIGGPKNQ
                     LVGDLHAAVNSNDALALAVNTGHYLRRPAVETASVSMDVQAPSNFERLVFEASGRHAS
                     GTQALFNDFNATGDVRLSPDLQNALQAEVSASRVDEATTKATIKTVYERYGKVICPHT
                     AVAVAAAMERGLDKPQIALATAHPAKFPGFVTEALGFAPEVPEAIAKLHGQKETIRDI
                     RNDEFAALEVVKAFAH"
     gene            1657..>1803
                     /locus_tag="VF402_02925"
     CDS             1657..>1803
                     /locus_tag="VF402_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX7799275.1"
                     /translation="MSEVAKRLDDLLEEFDLFDDWEERYRYIIDLGKDLAPLSEDEKT
                     EATRV"
BASE COUNT          314 a          607 c          599 g          283 t
ORIGIN      
        1 atgtcggcgg ccagccccgc catccatgca tcgcgcgtcc gtcccgtcgc ggcgtccacc
       61 accagcgccg agccgacctt gaccgtcagg cgcgccccct gcgccaggac ttgtggcaaa
      121 tttgcggaca aggtcatcta aaagtctttt cccaaacggc ataaggtgct aggccgcgcc
      181 ttcaaaagcc tgcgcgcttt tagcggcaaa gccagagggc gcgcaagcat ctaccttcgt
      241 cggacaggcg ctacagatga attacattgg cacccgaggc ttttcagaac cgcgcagctt
      301 cgccggcgcg ctgctggacg ggctggcgcc cgacggcggg ctttatgtgc cgcagaccta
      361 tccgcagccc ggtttcgacc ttgccgccgc cgcggcgggc gattacggcg cgctgaccga
      421 aaagctgctc accctgttcg gcgtcgacgt gctgggcgcc gaggcggtga cggcggcggc
      481 ggcgcgcacc aaggccgcct tcctgcgcat gacgccttcc ggggggcaga cgccgctgac
      541 gcaactggac gacaatctgt ggctgctgga gctgtatcac ggcccgaccc tggccttcaa
      601 agacatggcc atgcagatga tcgcgccgct gaccgacgcg gcgctggcgc agaccggcga
      661 gaagctgacc ctggtgaccg cgacctccgg cgacaccggc gcggcggcgg tgcgggcgtt
      721 cgcggggtcg gcgcatgtgc ggctggtggt gtttcatccg ctgggccgcg tctcgccggt
      781 gcagcgtctg caaatgacca ccgtggacgc cgacaatgtg ctgaacgtcg gggtcgaggg
      841 cgatttcgac gactgccagc gcatcgtcaa gcgcctgctg ggcgaggcgt ccctgaagga
      901 acgcggcctg atctccagcg tgaattcgat caactggggc cgtctgttgg ggcaggttcc
      961 ctactatctc gccgccagcg cggccagcgg cgcggacagg cccgatttcg tcgcgccgac
     1021 cggcaatttc ggcgacgcct tcgcgggctg ggtcgcgcgc aggatcggcg gaccaaagaa
     1081 ccaacttgtc ggcgacctgc acgcggcggt caacagcaac gacgcgctgg cgctcgccgt
     1141 caataccggc cattacctgc gccgtccggc ggtcgaaacc gcctcggtgt cgatggacgt
     1201 gcaggcgccg tccaatttcg agcggctggt gttcgaggcg tccggccgtc acgccagcgg
     1261 cacgcaggcc ctgttcaacg atttcaacgc caccggcgac gtgcgcctgt cgcccgacct
     1321 gcaaaacgcc ctgcaagccg aggtgtcggc cagccgcgtc gacgaagcga cgaccaaggc
     1381 gacgatcaag acggtgtatg agcgttacgg caaggtgatc tgtccgcaca ccgccgtggc
     1441 ggtcgcggcg gcgatggaac ggggtctgga caagccgcag atcgccctgg ccaccgcgca
     1501 tccggccaag ttccccggct tcgttaccga ggcgctcggc ttcgcgccgg aagtccccga
     1561 agcgatcgcg aagctgcatg gccagaagga aaccatccgc gacatccgca acgacgagtt
     1621 cgccgcgctg gaagtcgtca aggcgttcgc gcactgatgt cggaagtcgc aaaacgcctc
     1681 gatgacctgc tcgaagaatt cgacctgttc gacgactggg aggagcgcta ccgctacatc
     1741 atcgacctcg gcaaggatct cgcgccgctc agcgaggatg aaaagaccga ggcgacgcgc
     1801 gtg
//
LOCUS       DASVLL010000177         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_63817, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000177 DASVLL010000000
VERSION     DASVLL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63817"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /gene="eno"
                     /locus_tag="VF402_02930"
     CDS             complement(<1..682)
                     /gene="eno"
                     /locus_tag="VF402_02930"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX7799276.1"
                     /translation="MANRIKTIRSLEVLDSRGFPTVRTWVELDGGAVGVATVPSGAST
                     GENEAVELRDGDRNRYGGKGVLKAVANVETVIATALTGMDAADQRGLDEAMIALDGTR
                     NKAKLGANAILSVSMAAACAAAKAEGCPLYRYLDRGFGLKLPVPMMNILNGGKHADNS
                     VDFQEFMVMPLGAPRFSEAVRYGAEVFHALKAILHRKGYATSVGDEGGFAPDLTSNDE
                     ACDLIVEAI"
     gene            complement(675..1214)
                     /gene="ppa"
                     /locus_tag="VF402_02935"
     CDS             complement(675..1214)
                     /gene="ppa"
                     /locus_tag="VF402_02935"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX7799277.1"
                     /translation="MDLSKIPAGVAPPDDINVIIEIPQGGTPVKYEMDKASGALFVDR
                     FLHASMTYPANYGFIPDTLSADGDPCDALVICQQPVATGSVIRSRPIGALLMEDEHGK
                     DEKILAVPVDRLHPYYSQVRSHEDLPRILLDQIAHFFQHYKDLEKGKWVEITRWVGAD
                     AARNLIMDAVKAAQETHHG"
     gene            complement(1342..1689)
                     /locus_tag="VF402_02940"
     CDS             complement(1342..1689)
                     /locus_tag="VF402_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX7799278.1"
                     /translation="MTTPLSGTASLMRVFVGERDSLEGKPVFEWIVLKARESGIAGAT
                     VLRSPMGYGHSSHVHNATILRLSTDLPMVVELIDVEARILAFADLLKASLKGGLITLE
                     KITVIPLGQEPRD"
     gene            complement(1686..>2085)
                     /gene="crcB"
                     /locus_tag="VF402_02945"
     CDS             complement(1686..>2085)
                     /gene="crcB"
                     /locus_tag="VF402_02945"
                     /inference="COORDINATES: protein motif:HMM:NF010802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX7799279.1"
                     /translation="AYLWVGLGGALGAMLRYWLSGVVATAWGETFPWGTLIINVTGSI
                     FISFFATLTGPDGRAIVSPDFRTFVMIGICGGYTTFSSFSLQTLNLARNGEIAAAGWN
                     SLLSFGLCLLGAWLGYLGAAFINQLEGVRL"
BASE COUNT          367 a          674 c          682 g          362 t
ORIGIN      
        1 ggatcgcctc cacgatcagg tcgcacgcct cgtcattgga ggtcagatcg ggcgcgaagc
       61 cgccctcgtc gcccaccgag gtcgcatagc ccttgcgatg caggatcgcc ttcagcgcat
      121 gaaagacctc ggcgccgtag cgcacggctt cggaaaagcg cggcgcgccc agcggcatga
      181 ccatgaattc ctgaaaatcg acgctgttgt cggcgtgctt gccgccgttg aggatgttca
      241 tcatcggcac gggcagcttc aggccgaaac cgcgatcgag atatctgtaa agcggacagc
      301 cctccgcctt cgccgccgcg cacgccgccg ccatcgacac gctgaggatg gcgttggcgc
      361 cgagtttcgc cttgttgcgc gtgccgtcga gcgcgatcat ggcttcgtcc agcccgcgct
      421 gatccgccgc gtccatgccg gtcagggcgg tcgcgatgac ggtttcgaca ttggcgaccg
      481 ccttcaggac gcccttgccg ccatagcggt tcctgtcgcc gtcgcgcagt tcgacggctt
      541 cgttctcgcc cgtggaggcg cccgacggga ccgtggcgac gccgacggcc ccgccgtcca
      601 gctcgaccca tgtgcgcacc gtgggaaagc cgcgcgagtc gaggacttcg agcgagcgga
      661 tggtcttgat gcggttagcc atgatgcgtc tcctgcgccg ccttgacggc gtccatgatg
      721 aggttgcggg cggcgtccgc gccgacccac cgggtgatct cgacccactt gcccttttcg
      781 agatccttgt agtgctgaaa gaagtgcgcg atctgatcga gcaggatccg gggcaggtcc
      841 tcatgactgc gcacctggct gtaataggga tgcagccggt cgaccggcac ggcgaggatc
      901 ttttcgtcct tgccatgctc gtcctccatc agaagggcgc cgatcgggcg cgagcggatg
      961 accgaaccgg tggcgaccgg ctgctggcag atcacgagcg cgtcgcacgg atcgccgtcc
     1021 gcggacaacg tatcggggat aaatccgtag ttcgcgggat aggtcatcga ggcgtgcagg
     1081 aagcgatcga cgaacagggc gccggacgcc ttgtccatct cgtacttgac cggcgttccg
     1141 ccttgcggaa tctcgatgat gacattgatg tcgtccggcg gcgcgacgcc cgccggtatc
     1201 ttgctcaggt ccatgacgtc cgtttcttcc tgtatgaaaa gggcgagtcc gcgcaggggg
     1261 caaacccgcg cccggccggg aaacacggtc ggccgggccg ccccggaggt cgggaacggc
     1321 ggactcatga aaccgcccgc atcagtcccg cggctcctgt ccgagcggga tgacggtgat
     1381 tttttccagc gtgatcaggc cgcctttcag ggacgccttg agcaggtcgg cgaaggcgag
     1441 aatgcgcgcc tcgacgtcga tcagctcgac caccatcggc aggtcggtgg acaatctcag
     1501 gatcgtggcg ttgtggacat ggctggaatg gccatacccc atcggactgc gcagcaccgt
     1561 ggcgccggcg atgccggatt cgcgcgcttt cagcacgatc cactcaaaga cgggcttgcc
     1621 ttcgaggctg tcgcgctcgc cgacgaagac gcgcatcagc gaggcggttc cggataaggg
     1681 cgttgtcata gcctaacgcc ctccagctgg ttgatgaagg ccgcgccgag atagcccagc
     1741 caggcgccga gcaggcatag gccgaacgac aacaggctgt tccatcccgc cgccgcgatt
     1801 tcgccgttgc gcgccagatt cagggtttgc aggctgaagg acgaaaaggt ggtgtaaccg
     1861 ccgcagattc cgatcatcac gaaggtgcgg aagtcgggac tgacgatggc ccttccgtcc
     1921 ggtccggtca gcgtggcgaa gaagctgatg aagatcgagc ccgtgacgtt gatgatcagg
     1981 gtgccccacg gaaacgtctc gccccacgcc gtcgccacga cgcccgacag ccaataccgg
     2041 agcatcgcgc ccagcgcgcc gccgagtcca acccatagat aagcc
//
LOCUS       DASVLL010000178         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_440725,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000178 DASVLL010000000
VERSION     DASVLL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440725"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF402_02950"
     CDS             complement(<1..260)
                     /locus_tag="VF402_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799280.1"
                     /translation="MLQDFFERIPPELMQESGSVFYSGRAAFSKPAPVYLLGVNPGGS
                     PASQQEPRIGTHCRSVLYDENYENWSAYLDLAWGARTEKGGAP"
     gene            551..1192
                     /locus_tag="VF402_02955"
     CDS             551..1192
                     /locus_tag="VF402_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7799281.1"
                     /translation="MILIGQYDSPFVRRVAIAMTLYGVAFRHRPWSTFGDGDKIAAYN
                     PLRRAPTLILNDGRVIIESAYILDWLDEQVRDGDADVMPLIAAAGPERRTTLYYTALA
                     TGFADKAVALFYETVLHEVHSPVWLERCRTQLAAVLDQLETIRVAAKSRWLQGAEMTH
                     ADIALGCALRHARDAHGGTIGWEDWPALAFHSAQCEELDVFKAIQQPFAAPKV"
     gene            1250..2422
                     /locus_tag="VF402_02960"
     CDS             1250..2422
                     /locus_tag="VF402_02960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7799282.1"
                     /translation="MTAHSDIKAAPSVLRIETLRPQDLTAEDARLWRSFLARRPELSG
                     PYFDIRYVQAAASAVPDAGVARFYDEDRVVGYFPYQIRSGALQPFGAPLSDYHGVIAA
                     PGCDMPFGELLAATGARRLEFQGWIGPMCERARALPLRRRVAETSRGFAHWWATQDAE
                     HHKFFKNIGRCERNVEKDFGGFAFTWERVTPDVLDWVLRLKREQYRKSGMHDVFDCGW
                     TYAMLSALAALDDPDYGLRAGVFRHEGRIVAAEISLTDAEHVHLWFPAYDPAYYRYSV
                     GILLTVAIIRHCAPLGIKSFDFGTGGEEYKSPLTTDGGGCLEGNLKYAPRLGSQVLDM
                     TARALPVGGARFTQARLSLKRRVNLIRATETGLAGWGRAGLSLMRRAVMRLTPGRS"
BASE COUNT          447 a          833 c          942 g          525 t
ORIGIN      
        1 ggagcgccgc ctttctcggt tcgggcgccc catgcgagat ccagataggc ggaccagttt
       61 tcgtaatttt cgtcatacag gacggaccgg caatgcgtcc cgatgcgtgg ctcctgctgc
      121 gaagccggag agcctccggg attgacgccc agaagataaa cgggagcggg ttttgaaaac
      181 gccgcccgcc cggaataaaa gacggaaccc gattcttgca tgagttcagg gggaatgcgc
      241 tcaaaaaaat cttgtagcat attgcgtccg tacacctgtt gcggtcagcg cgatttcgac
      301 ggctccataa catcggcgcg gacgtaaagg tcgcgttaag ccgccattgg cggcgcgacc
      361 catgcgccct tttatcgcct tttgttttga caagcccgac cggaccgtga cctcatagtc
      421 ccgctgggtt tggagacaag tcgatgcgca agctcatcct gttggccctg atcgccgtcg
      481 cctgcgccgg gtgcgcgcag gggggctggt atggcggcgg cggcatcggc gctttggcgg
      541 aggcgcggcc atgatcctga tcggtcagta cgattcgccg ttcgtgcgcc gtgtggccat
      601 cgccatgacg ctgtacgggg tcgcgttccg gcatcgtccg tggtcgacct tcggcgacgg
      661 ggacaagatc gccgcctaca atccgttgcg gcgcgcgccg accctgattc tgaacgacgg
      721 gcgggtgatc atcgaaagcg cctatattct cgactggctg gacgagcagg tcagggacgg
      781 ggacgccgac gtcatgccgc tgatcgccgc cgccgggccg gagcggcgga cgacgcttta
      841 ttatacggcg ctggcgaccg ggttcgcgga caaggcggtc gcgctgtttt acgagacggt
      901 cctgcacgag gtccattccc cggtgtggct ggagcgctgc cgcacgcagc ttgccgccgt
      961 gctcgatcag ctcgaaacga tccgcgtcgc cgccaaaagc cgttggctgc aaggggcgga
     1021 gatgacgcat gccgacattg ccctgggttg cgctttgcgt cacgcgcgag acgcgcacgg
     1081 cgggacgatc ggctgggagg actggcccgc cctggccttc catagcgcgc aatgcgagga
     1141 actagacgtt ttcaaggcga tccagcagcc gttcgccgcg ccgaaagttt aaggcgtgtt
     1201 aaccctgtcg cttaggggcg tggcaatctg atcgtgctag ggttgcgcca tgacagccca
     1261 tagcgacatc aaggccgcgc cttccgtgct ccgcatcgag accctgcgtc cgcaggattt
     1321 gacggcggag gacgcccgcc tgtggcggtc gttcctggcg cggcgcccgg agctgagcgg
     1381 cccctatttc gatatacgct atgtgcaggc ggcggcttcg gccgtgcccg atgcgggcgt
     1441 ggcgcgcttt tacgacgagg atcgcgtcgt cggctatttc ccgtatcaga tccgctccgg
     1501 cgccttgcag ccgttcggcg cgccgttgag cgactatcac ggcgtcatcg cggcgcccgg
     1561 ctgcgacatg ccgttcggcg agcttctggc cgccacgggg gcgcggcggc tggagtttca
     1621 gggctggatc gggccgatgt gcgaacgggc gcgcgcgctg cctttgcgcc gccgcgtggc
     1681 cgagacgagc cggggcttcg cgcattggtg ggcgacgcag gacgccgagc atcacaagtt
     1741 cttcaagaat atcggccgct gcgagcgcaa tgtcgaaaag gatttcggcg gcttcgcctt
     1801 cacgtgggag cgcgtgacgc cggacgtgct ggactgggtg ctgcggctca agcgcgagca
     1861 gtaccgcaag agcggcatgc acgacgtgtt cgactgcggc tggacctacg ccatgctgtc
     1921 ggcgctggcg gcgctggacg atcccgatta cggcctgcgc gcgggcgtgt tccggcacga
     1981 gggccgcatc gtcgccgccg agatttcgct gaccgacgca gagcatgtgc atctgtggtt
     2041 tccggcctac gacccggcct attaccgcta cagcgtcggc atcctgctga ccgtggcgat
     2101 catccggcat tgcgcgcctc tgggtataaa gagtttcgat ttcggcacgg gcggcgagga
     2161 gtacaagtcg ccgctgacca cggacggcgg cgggtgtctc gaaggcaatc tgaaatacgc
     2221 gccgcgcctc ggctcgcagg tgctggatat gacggcgcgc gccctgccgg tgggcggcgc
     2281 gcgctttacg caggcgcgcc tgagcctgaa acgccgcgtc aacctgatcc gcgccaccga
     2341 aaccggtctg gccgggtggg gcagggcggg gctgagcctg atgcgccgcg ccgtcatgcg
     2401 cctgacgccg gggcggtcgt aggcggatca tcacgcgctt attttttccg ggattcgccg
     2461 tattcggggt tgactaggct gagtcctttg ggcatatagc gcgctctgtt ggatcggctg
     2521 tggcgcggcg acgctgccaa acgcggtctg caagcctaca ccaggttcgc tggcggcctt
     2581 ttttaaaagg aagatgcgaa tttgataatg gcccgcgcgg cgacaaaccg cgagggcttt
     2641 gtttttgcgg ataagcgttc gtgatcccgt ctcgggagat cgaagccggt ctttgaaatg
     2701 gttcacggaa cgcgttagtc gcaacctaac aggaatacga gcgatat
//
LOCUS       DASVLL010000179         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_279634,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000179 DASVLL010000000
VERSION     DASVLL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279634"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /gene="lnt"
                     /locus_tag="VF402_02965"
     CDS             <1..1479
                     /gene="lnt"
                     /locus_tag="VF402_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7799283.1"
                     /translation="LGPYPKRTAFFMGWLAGFFYFLVSCFWVAEAFLVDAATYGWMAP
                     FAVMLLPAGVGLFWGAFGVIYSRFRPAGAKRFVFFACAFTTFEYLRGMALSGFPWDPA
                     GSTWRAGDAMSQTAALVGVYGLGFLTVLIFASLAVIRRGRGWRGYAPALFGAGLFLAC
                     FAFGEVRLRTTPVTFSDYTVRIVQPDIGQAAKWDADNFGDLFDAYVTMSKAAPAPGHK
                     APDLIVWPEGALPFPAEAVFSADAWTAPVMADLLRQNQSLIVGFSRRDITPQGRDVWR
                     NSLLVLTRHQDRTVIEGAYNKFKLVPFGEFTPFESVLKPLGVEALTHFDDSFTRGART
                     RPMSVGAIPRFLPLICYEGIFPSLDQRLYRNNGDKMRPKWIVNISNDAWFGPTSGPRQ
                     HLNLASYRAIEEGLPMIRSTPTGISVLVDPLGRVVKGSEIGMGQRGYRDVRLPEPAPV
                     TAYEGQRNVSLWLITILGLIIAGFDTCMPWIKRNLVTKKSSR"
     gene            1553..>1819
                     /locus_tag="VF402_02970"
     CDS             1553..>1819
                     /locus_tag="VF402_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7799284.1"
                     /translation="MDENFKADRGPNPVDLHVGARVRMRRKFLGMSQEGLAETIDLTF
                     QQVQKYERGSNRISASKLWEIAKALKAPVAYFFEGYGENEAVEGF"
BASE COUNT          318 a          563 c          571 g          367 t
ORIGIN      
        1 ctggggccgt atccgaagcg cacggccttc ttcatgggct ggctggccgg attcttctat
       61 tttctcgtca gttgcttctg ggtggccgag gcgtttttag tcgacgcggc cacctatggc
      121 tggatggcgc cgttcgcggt catgctgctg cccgccgggg tcgggctgtt ctggggcgcg
      181 ttcggcgtga tctacagccg cttccggccc gcgggcgcca aacgtttcgt gttcttcgcc
      241 tgcgccttca ccacgttcga atatctgcgc ggcatggcgc tgtccggctt tccgtgggat
      301 ccggcgggat cgacctggcg cgcgggcgac gcgatgtcgc agacggcggc gctggtcggg
      361 gtttacggcc tcggcttcct taccgtgctg atctttgcca gcctcgccgt catccggcgc
      421 gggcggggct ggcgcggcta tgcgcccgcg cttttcggcg ccggtctgtt cctggcctgc
      481 ttcgcgttcg gcgaagtccg gctgcgcacg acgccggtga cgttcagcga ctatacggtg
      541 cgcatcgtgc agcccgacat cggtcaggcg gccaagtggg acgccgacaa tttcggcgac
      601 ctgttcgacg cctacgtcac gatgagcaag gcggcgcccg cgcccggcca caaggcgccc
      661 gacctgatcg tctggcccga aggcgcgctg ccgtttcccg ccgaggcggt gttctcggcc
      721 gacgcctgga ccgcgccggt catggcggac ctgctgcgcc aaaaccagtc tctgatcgtc
      781 ggtttcagcc gccgcgacat cacgccgcag ggccgggacg tgtggcgcaa ttcgctgctg
      841 gtcctgaccc gtcatcagga ccggaccgtc atcgaaggcg cctacaacaa gttcaagctg
      901 gtgccgttcg gcgagttcac gccgtttgaa agcgttttga aaccgctggg cgtcgaggcc
      961 ctgacccatt tcgacgacag cttcacgcgc ggcgcgcgca cgcggcccat gagcgtcgga
     1021 gccataccgc gcttcctgcc gctgatctgc tacgaaggca tcttccccag cctggatcag
     1081 cgcctgtacc gcaataacgg cgacaaaatg cggcccaaat ggatcgttaa catctccaac
     1141 gacgcatggt tcggtccgac cagcggcccc cggcaacacc tcaatctggc cagttatcgc
     1201 gcaatagaag aggggctgcc catgataaga tcaactccga cgggaatatc tgttctggtc
     1261 gatcccctcg gacgtgtggt caaaggcagc gaaatcggca tgggtcagcg cggttataga
     1321 gatgtaaggc tgcccgaacc ggcgccggtt accgcatacg aagggcaacg caatgtcagc
     1381 ctctggctta ttactattct gggcttgatt attgctggat ttgatacttg tatgccgtgg
     1441 atcaaaagaa acctagtgac gaaaaaatcg tcgcgttagg aactgttccg cccccgctgg
     1501 acgtataaat tgatccggcc ctgacaggcc gttcagatga taagaggcta agttggacga
     1561 aaactttaaa gcggaccgtg gccccaaccc ggtcgatttg cacgtcggcg cgcgcgtcag
     1621 gatgcgccgc aagtttctgg gtatgagtca ggaaggtctg gccgaaacca tcgacctcac
     1681 ctttcagcag gtgcagaagt atgagcgcgg ttccaaccgc atcagcgcgt cgaagctgtg
     1741 ggaaatcgcc aaggcgttga aggcgccggt cgcctacttc ttcgaaggct acggcgaaaa
     1801 cgaagccgtc gagggattc
//
LOCUS       DASVLL010000180         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_494663,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000180 DASVLL010000000
VERSION     DASVLL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494663"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..974
                     /gene="pstB"
                     /locus_tag="VF402_02975"
     CDS             165..974
                     /gene="pstB"
                     /locus_tag="VF402_02975"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7799285.1"
                     /translation="MTDTTSPDFKSVIVPPPADSLVLEVKKLDFFYGAHQSLFGVDLP
                     VKRNAITALIGPSGCGKSTLLRTINRIYDLYPGQRAEGEILFDGEDILNGKADVAALR
                     ARIGMVFQKPTPFPMSIYDNVAFGVRLHQSKSKSEMDELVESSLRKAALWDEVKDKLT
                     KSGLGLSGGQQQRLCVARGIAVNPEILLLDEPASALDPVSTAKLEDTLEELKRDYSIV
                     IVTHNLQQAARLSDYTGFMFLGKMIEFGPTEELFVKPKVDKTGEYIGGRFG"
     gene            complement(1176..3170)
                     /locus_tag="VF402_02980"
     CDS             complement(1176..3170)
                     /locus_tag="VF402_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7799286.1"
                     /translation="MPEFYAPNETPPHTPLFGDDRRLKQVALALWAVMLVWKFAAGLI
                     SHVIWEESHFVVSGQYLAFGYPDIPAGFPWLARLITTLFGWHVWPLRIVALAIGMAIP
                     WAVWFMAKPVTTQRNAIWAAIIAMLLPAIALNGTIFYPEGALQLLLALMLGCILRGMR
                     EPGLKWWLWAGVCAGIGQLVHFRFLVPGLAVVVFMLAEPRGRRLWTHPGVWITAALGV
                     VGLIPALLYNAAEGWPAIEFHVIRRPNYDLEPKHVLSLLATQFGVWTPAFFIAIWGAV
                     KHALWPKKDGQRAERGPETLLAWQALVIFVFYALEATVNKKVMPHWPFMAAVPMLPMV
                     PGVLRRFADGARSPGARRFRAALIATGPLIGVAIGVAATGYQILYDNSAKLNSQGRAY
                     NILKNEDWTLLDAPLRQAMARAEARFGAEPAVATNGHIAAVHLEFPADGHRDLRVYTL
                     GDPYDDVSMFSVARRDWSLDRQALLRNHAGGPVVLALLEPSYIYHEPEQVALYTNLCK
                     DFESVEREGITALPPYRTAIDFYTAKVRATPLSDAQVDAAPCPFVPQLYIAHPTRGDF
                     IKRDDTRIKTGMAADPKGVKAVDILLDGKVVAHAHYGIDEPTHRAPDLLRYDPNWPNL
                     QFTYSFPKGSLKVGTHTLSARALRSDGTSFEADPRVLYVE"
     gene            complement(3229..4080)
                     /locus_tag="VF402_02985"
     CDS             complement(3229..4080)
                     /locus_tag="VF402_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7799287.1"
                     /translation="MRSVITALLTLAIAAPASAQTVIPLWAKGQPGFESRRDIPEQHA
                     DWWWRSINDPSLTVFAPAAGKNTGTAVIVVPGGGHQNLVFHAEGEAPGQYLAGIGVTA
                     FALKYVLAREKGSPYSIEGDAKADLVRAIRYVRAHAADYGIDPHRIGVMGFSAGGELT
                     SMAAFNPPAADPNAADPVDRTDARPDFLIEIYPGPLGIVPFTDHAPPPVFLLNGSADR
                     MAMADTDAIERLYDAFPDVPFERHVMASVPHAFNMGDRSTRKTVRDWPQRLTDWLSDM
                     GLLTPAK"
     gene            complement(4224..>4377)
                     /locus_tag="VF402_02990"
     CDS             complement(4224..>4377)
                     /locus_tag="VF402_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX7799288.1"
                     /translation="YGEFDSLEDFRAYKAHPLYEESIRQVRHLRNIRVAADYTVSSAT
                     RELIKG"
BASE COUNT          734 a         1380 c         1529 g          734 t
ORIGIN      
        1 ccctgccggt cgtcatgttc aacatggcgc tcagccccta cagcgactgg aacaggctgg
       61 cctgggccgc cgcgcttctg gtggccgtcg ccgtccttat cgtcaacatt gtcgggcgct
      121 ggctggcgcg cgagaaacaa cagcattaac cgggcctcag actcatgacc gacaccacgt
      181 cccccgactt caagagcgtg atcgtcccgc ctccggccga cagcctcgtg ctcgaagtga
      241 aaaagctcga cttcttctac ggcgcgcacc agtcgctgtt cggcgtcgac ctgccggtga
      301 agcgcaacgc catcaccgcc ctgatcggcc cgtcgggctg cggcaagtcg acgcttctgc
      361 gcaccatcaa ccgcatctac gacctctatc cgggccagcg cgccgagggc gagatcctgt
      421 tcgacggcga ggacatcctg aacggcaagg ccgacgtcgc cgccctgcgc gcccgcatcg
      481 gcatggtgtt ccagaagccg accccgttcc cgatgtcgat ctacgacaat gtcgcctttg
      541 gggtgcgcct gcatcagtcg aagtcgaagt ccgagatgga cgagctggtc gaatcctccc
      601 tgcgcaaggc ggccctgtgg gacgaggtca aggacaagct gaccaagtcg ggcctcggcc
      661 tgtcgggcgg gcagcagcag cgcctgtgcg tggcgcgcgg catcgcggtc aacccggaga
      721 tcctgctgct ggacgaaccg gcctcggcgc tcgacccggt ctcgaccgcc aagctggagg
      781 acacgctgga ggagttgaag cgcgactatt cgatcgtgat cgttacgcac aacctgcaac
      841 aggcggcgcg cctgtccgac tatacgggct tcatgttcct aggcaagatg atcgagttcg
      901 gcccgaccga ggaactgttc gtcaagccga aggtcgacaa gaccggcgaa tatatcggcg
      961 gccgcttcgg gtaggttatc acaaaatctt cccctctccc catgtccatg gggagaggac
     1021 gaggagcaag gcgcagccgc cgatcctcgg gtgaggggct ggcgacggtt gaaacggcgg
     1081 acagcccctc aggctgcgcg ccccgcccgt cgccttcgcc tgacggctcg gctctcgtcc
     1141 tctccccatg tgcatgggga gaggggtagt tttttttatt ccacgtacag cactcttggg
     1201 tcggcttcga agcttgtgcc gtcgctgcgc agggcgcggg ccgagagggt gtgggtcccg
     1261 accttcaaag accctttcgg gaagctgtag gtgaattgca ggttcggcca gttcgggtcg
     1321 tatctcaaca ggtccggcgc gcggtgcgtc ggctcgtcga tgccgtaatg cgcgtgggcg
     1381 accaccttgc cgtccagtaa aatatcgacc gccttcacgc ccttcgggtc ggcggccatg
     1441 ccggtcttga tcctcgtatc gtcgcgtttg atgaagtcgc cgcgcgtcgg gtgggcgatg
     1501 tagagctgcg gcacgaacgg gcagggcgcc gcatcgacct gcgcgtcgct cagcggcgtc
     1561 gcgcgcacct tggcggtata gaagtcgatg gcggtgcggt agggcggcag ggcggtgatc
     1621 ccttcgcgct cgaccgactc gaaatccttg cacaggttgg tgtagagcgc gacctgctcc
     1681 ggctcgtgat agatgtagga cggctccagc agcgccagca cgaccggccc gcccgcatgg
     1741 ttccgcaaaa gggcctgccg gtcgaggctc cagtcgcggc gcgccaccga gaacatgctg
     1801 acgtcgtcat agggatcgcc cagcgtatag acgcgcagat cgcgatggcc gtcggcgggg
     1861 aactccagat gcacggcggc gatatggccg ttggtcgcca cggcgggttc ggcgccgaag
     1921 cgggcttcgg cgcgcgccat cgcctgacgc agcggcgcgt cgagcagggt ccagtcctcg
     1981 tttttcagga tgttgtaggc gcgcccttgc gagttgagct tcgccgaatt gtcgtagagg
     2041 atctgataac cggtcgccgc gacgccgatg gccacgccga tcagcggccc cgtcgcgatc
     2101 agggcggcgc ggaaacggcg ggcgccgggg ctgcgcgcgc cgtcggcgaa gcggcgcagc
     2161 acgcccggaa ccatcggcag catcggcacg gcggccatga acggccagtg cggcatgacc
     2221 ttcttgttga ccgtggcttc cagcgcgtaa aagacgaaga tgaccagggc ctgccacgcc
     2281 agcagggttt ccgggccgcg ctccgcgcgc tggccatcct ttttcggcca gagcgcatgt
     2341 ttgaccgcgc cccagatggc gatgaagaag gcgggcgtcc acacgccgaa ctgcgtcgcc
     2401 agcaggctca ggacgtgctt cggttcgagg tcgtaattgg ggcggcggat gacgtggaac
     2461 tcgatggcgg gccagccctc ggcggcgttg taaagcaggg ccgggataag cccgacgacg
     2521 ccgagcgccg ccgtgatcca cacgcccgga tgcgtccaca gcctgcgtcc gcgcggctcg
     2581 gccagcatga agaccacgac cgccagcccc ggcacgagga agcggaaatg caccagttgc
     2641 ccgatcccgg cgcatacccc cgcccagagc caccatttca gccccggctc gcgcatgccg
     2701 cgcaggatgc agcccagcat cagggccagc agcagttgca gggcgccttc gggatagaag
     2761 atggtgccgt tgagcgcgat ggcgggcagc agcatggcga tgatcgccgc ccagatcgcg
     2821 ttgcgctgcg tcgtcacggg cttcgccatg aaccagaccg cccacgggat cgccatgccg
     2881 atggccaggg ccacgatgcg cagcggccag acatgccagc cgaaaagcgt ggtgatcagc
     2941 cgcgccagcc acggaaagcc cgccggaatg tcgggatagc cgaaggccag atattgcccg
     3001 gagacgacga agtggctttc ttcccagatg acgtggctga tcaggccggc ggcgaacttc
     3061 cagaccagca tgacggccca cagggccagg gcgacctgtt tcaggcgcct gtcgtcgccg
     3121 aacaacggcg tgtgcggcgg cgtttcgttc ggtgcgtaaa actccggcat ggcgtctttc
     3181 cctgttcgcc gcccttttgg caggccgcgg gagcgaggta aagggccgct atttcgcggg
     3241 cgtcagcagg cccatgtcgg acagccagtc ggtcaggcgc tgtggccaat cgcgcacagt
     3301 tttgcgcgtg gagcggtcgc ccatgttgaa ggcgtgcggc acggaggcca tcacatgccg
     3361 ctcgaacggc acgtcgggga aggcgtcgta caggcgctcg atggcgtcgg tgtcggccat
     3421 cgccatgcgg tccgccgagc cgttgagcaa aaacaccggc ggcggcgcgt ggtcggtgaa
     3481 cggaacgatg cccagcgggc cgggatagat ttcgatcagg aagtccgggc gcgcgtcggt
     3541 ccggtcgacg ggatcggcgg cgttcgggtc ggcggcgggc ggattgaagg ccgccatcga
     3601 ggtcagctcg ccgcccgccg aaaagcccat cacgccgatg cggtgcggat cgatcccata
     3661 gtcggcggcg tgggcgcgca cgtagcggat ggcgcgcacg aggtcggcct tggcgtcgcc
     3721 ttcgatggag tagggcgagc ccttttcccg cgccagcacg tatttgagcg cgaaggcggt
     3781 gacgccgatc ccggccagat attgtcccgg cgcctcgccc tcggcgtgga agacgaggtt
     3841 ctgatgcccg ccgccgggga cgacgatgac ggcggttccg gtgtttttac cggcggcggg
     3901 cgcgaacacg gtcagggacg gatcgttgat cgagcgccac caccagtcgg cgtgctgttc
     3961 agggatgtcg cggcgcgatt cgaagcccgg ctggcccttg gcccataacg gtatgacggt
     4021 ctgcgcggag gcgggcgcgg cgatggcgag ggtgagcaga gcggtgatga cggaacgcat
     4081 ggcgtcctcc cggttttatg gttttgggag gagactagcg tattatgagg gagatggcga
     4141 tgcgcttcgt ggcgtggcca agacctctgc gtcgttcgcc gcgcaaacgc cgctccccgt
     4201 ggcatgggca ggtgggcggg gggtcagcct tttatgagtt cgcgggtggc ggacgagacg
     4261 gtgtagtcgg cggcgacgcg tatgttgcgc aggtggcgca cctggcggat cgattcttcg
     4321 tagagcggat gggccttgta ggcgcggaag tcttcgagcg aatcgaactc gccatag
//
LOCUS       DASVLL010000181         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_333092,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000181 DASVLL010000000
VERSION     DASVLL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333092"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1573)
                     /locus_tag="VF402_02995"
     CDS             complement(155..1573)
                     /locus_tag="VF402_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX7799289.1"
                     /translation="MRRDVRDFGARGDGATLDTDAIRAAIAAAGPGDTLVFGRGHYLS
                     FSIGLKSHLHLVFEDGCVLEAADPERHGGHYDAAEDNPFDLYQDFGHSHWRNSLIYGI
                     DVEDVTLSGPGRIDGRGLTREGPGSRWRKQAGEFPESMRGMSAEQMAVLSPHVTEMAG
                     QGNKAVAFCRARGVRIRDLSIVNGGHFAVLATGVDDLLLETLNIDSNRDGIDIDGCRR
                     VIVRGCRVNTPNDDAIVLKSSFALGERRPCEDVLIEDCAVSGFDLGTMIDGTRACTQV
                     FSPDRDRVTGRIKIGTESSGDFRRIKINRCLFERSRGLALETVDGGVIEDVEATHLTL
                     REVTTAPLFLRLGARLRAPEGAVPGAIRRVAIRDMTAEDILPDYSALIVGMEGHPVED
                     VTLERVRLTYRGGGVAVDGSPPEKDDAYPEPSMFGRTPAYGLWTRHVRGLTLKEVALS
                     AETPDARPRTLFEDTTLHENGA"
     gene            complement(1574..>2063)
                     /locus_tag="VF402_03000"
     CDS             complement(1574..>2063)
                     /locus_tag="VF402_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX7799290.1"
                     /translation="GLEDYIGWLAANFDPGIAWSDLQWIRDAWDGPMVIKGILDPQDA
                     KDALVFGADGLVVSNHGGRQLDGAMSSARALPVIADAVKGDMTIFADGGVRTGLDVMR
                     MLALGADGVLIGRAFVYALAAAGEAGVRNLLALLEKDMKTCMVLTGCKSIADISPDIL
                     AL"
BASE COUNT          342 a          760 c          627 g          334 t
ORIGIN      
        1 gcgcagacca tcgacgatga cgtcgttgac gtcggcgtat tcgcccgatt cgatcttgtc
       61 gcgcaccagc cgcgccaggt cttcgggcag ggtgatattg aactgaacgt cgtcggccat
      121 gacggaaatc ctcacatttc cgctcagtct aacactaggc cccgttctcg tgaagcgtcg
      181 tgtcctcgaa cagggtccgc gggcgcgcgt cgggcgtctc ggcgctcagc gccacctcct
      241 tcagcgtcag gccgcgcaca tgccgcgtcc acagcccgta agcgggcgtg cggccgaaca
      301 tggacggctc cggataggcg tcgtcctttt ccggcgggga gccatccacc gccacgccgc
      361 cgccgcgata cgtcaggcgc acccgctcca gcgtcacgtc ctcgaccgga tggccttcca
      421 tgccgacgat caaggcgctg tagtcaggca ggatgtcctc cgccgtcatg tcgcggatcg
      481 cgacgcgccg gatcgcgccc ggaaccgccc cttccggcgc gcgcagccgc gcgcccagcc
      541 gcaggaacag gggcgccgtc gtcacctcgc gcaaggtgag gtgcgtcgcc tcgacgtcct
      601 cgatcacccc gccgtcgacc gtctccagcg ccagcccgcg cgagcgctca aataagcaac
      661 ggttgatttt gatgcgccgg aaatcgccgc tcgattcggt gccgatcttg atgcggcccg
      721 tcacccggtc gcggtcgggc gaaaagacct gcgtgcaagc gcgcgtcccg tcgatcatcg
      781 tgccgaggtc gaagcccgac accgcgcaat cctcgatcag cacgtcctcg cacggccgcc
      841 gctcgcccag cgcgaagctc gacttcagca cgatggcgtc gtcattgggc gtattgacgc
      901 ggcagccgcg cacgatcact cggcggcagc cgtcgatgtc gatcccgtcg cgattgctgt
      961 caatgttgag cgtctccagc agcagatcgt ccacgccggt cgccagcacg gcgaaatgcc
     1021 cgccgttgac gatgctgaga tcccggatcc ttaccccgcg cgcgcggcag aaggcgaccg
     1081 ccttgttgcc ctgcccggcc atctcggtca catgcggcga caacacggcc atctgttcgg
     1141 cggacatacc gcgcatgctt tcgggaaact cgcccgcctg tttgcgccag cgcgaccccg
     1201 gcccctcgcg cgtcaggccg cgtccgtcga tgcggcccgg cccggacagc gtcacgtcct
     1261 cgacgtcgat cccgtagatc aggctgttgc gccaatggct gtggccgaag tcctgataga
     1321 ggtcgaacgg attgtcctcg gcggcgtcgt aatggccgcc gtggcgctca ggatcggcgg
     1381 cctccagcac gcaaccgtct tcaaagacaa gatgaagatg gcttttcaac cctatggaaa
     1441 agctgagata atgccccctg ccgaacacaa gcgtatcgcc cggcccggcg gcggcgatgg
     1501 cggcgcggat cgcgtccgtg tccagcgtcg cgccgtcgcc cctggcgccg aaatcgcgca
     1561 cgtcgcgccg catctaaagc gccagaatat cggggctgat gtcggcgatc gatttgcagc
     1621 cggtcagcac catgcaggtc ttcatatcct tctccagcag ggccaaaagg ttgcgcaccc
     1681 ccgcctcgcc cgccgccgcc agcgcataga cgaaggcccg cccgatcagc acgccgtccg
     1741 cccccagcgc cagcatgcgc atcacgtcca gcccggtgcg cacgccgccg tcggcgaaga
     1801 tcgtcatgtc gcccttcacc gcgtccgcga tcacgggcaa ggcgcgcgcc gacgacatgg
     1861 cgccgtccag ttgccgcccg ccgtggttgg acacgacgag gccgtccgcg ccgaagacta
     1921 aagcgtcctt cgcgtcctgc ggatcgagaa tccccttgat caccatcggc ccgtcccagg
     1981 cgtcccggat ccattgcagg tccgaccacg caatgcccgg atcgaagttc gccgccagcc
     2041 agccgatata gtcctcaagc ccg
//
LOCUS       DASVLL010000182         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_333105,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000182 DASVLL010000000
VERSION     DASVLL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333105"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VF402_03005"
     CDS             complement(<1..1061)
                     /locus_tag="VF402_03005"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7799291.1"
                     /translation="MPTPENSAYIYDHARTPRGKGKKDGSLHDLTALNLSKQVLEAVR
                     DRNGLDTALVDDVLWGCVTPVGEQGGDIARAAVLAANYAETTGGVQLNRFCASGLEAV
                     NVAAAKVVSGEATMAIGGGVECMSRVPMGSDGGAWAIDVAAAFPTHFVPQGVSADMIA
                     TKWGFSRDDVDAYSVESHKRAAKSWAEGRFKHAIVPVKDQMGVIALDHDETIRPNTDM
                     QTLAGLNPSFAAFGEMAFDSVIQQRYPEIERVNHVHTPGNSSSIVDGAAAVLVGSQAA
                     GKITGLKPRARILGAASIGSEPSIMLTGPSYVTEKLLKKLGMKTTDIDIYELNEAFAA
                     VVLRYMQVLDIPHDKINVCG"
     gene            1271..2452
                     /gene="dgoD"
                     /locus_tag="VF402_03010"
     CDS             1271..2452
                     /gene="dgoD"
                     /locus_tag="VF402_03010"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEX7799292.1"
                     /translation="MDTSHTAKLNPGKTRLARIETFAVPPRWLFVRIETDDGAVGWGE
                     ASLEGHTEATLAAFAEIRERFIGHDAGRIEDMWQVFYRLGFYRGGPVLMSALSGFDQA
                     LWDLKARRLGVPVWQLLGGQVRERVQVYAWIGGDRPSEVVDAGRARLKQGFKAVKMNA
                     SEDMGWLDSPRVIDGVCERLTSVQALGLDVGLDFHGRVHKPMARQIAHALEPLRPLFI
                     EEPLLSEHPEAIAQFAAATTTPVALGERLYSRWDFKPFFERACVDIIQPDLSHAGGIS
                     EVRRIAAMAEAYDVAIAPHSPLGPLALGACMQIGIATPNHAIQEMSLGIHYNTNGHDL
                     LFYMKNPEVFDVKDGYVEALTGPGLGVEIDEDKVREMAKDCPPWRNPAWRGPDGYVRE
                     W"
     gene            complement(2490..3182)
                     /locus_tag="VF402_03015"
     CDS             complement(2490..3182)
                     /locus_tag="VF402_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX7799293.1"
                     /translation="MKHARIIALFAVGLTLGLGGCVNVLPKVKPVQLYRFGYHPDLLK
                     DKDAAAAAATTTAAPVPLMLGAISFPPASAGDQVMTTEDNEVSYVAQARWASSASALF
                     AAAVSEGFARSDQTVMLVPRGPTAADYRLDVSVRRFEASYSHGRPTVYIGVDARLLRL
                     SDRSVLAQRYISASVSVRRNDMTLISQGFDQATTEVVAGLTGFADASLKKAAPPPTPT
                     QPGDKGRKVEGL"
BASE COUNT          521 a         1068 c         1081 g          562 t
ORIGIN      
        1 ccgcagacat tgatcttgtc gtgcggaata tcgagaacct gcatgtagcg cagcaccacg
       61 gcggcgaagg cttcgttgag ttcgtagatg tcgatgtcgg tcgtcttcat gccgagtttc
      121 ttcagcagct tttccgtcac ataggacggg ccggtcagca tgatcgacgg ctccgagccg
      181 atggaggccg cgccaagaat gcgcgcgcgc ggcttcaggc cggtgatctt tcccgccgcc
      241 tgcgagccga ccagcaccgc cgcggcgccg tcgacgatgg aggacgaatt gcccggcgta
      301 tgcacatggt tgacgcgctc gatttcggga tagcgctgct gaatgaccga atcgaaggcc
      361 atttcgccga aggccgcgaa ggacgggttc agcccggcca gggtctgcat gtcggtgttc
      421 ggacggatgg tctcgtcgtg gtcgagcgcg atcacgccca tctggtcctt gaccggcacg
      481 atggcgtgtt tgaagcggcc ctcggcccac gatttcgcgg cgcgcttatg gctttcgacc
      541 gaataggcgt cgacgtcgtc gcgcgaaaag ccccacttgg tcgcgatcat gtcggcggac
      601 acgccctgcg gcacgaaatg ggtcgggaag gccgccgcca cgtcgatggc ccaggcgccg
      661 ccgtccgagc ccatcggcac gcggctcatg cactcgaccc cgccgcctat ggccatggtc
      721 gcttcgcccg acacgacctt ggcggcggcg acgttgacgg cctcaagccc ggaggcgcag
      781 aagcggttga gctgcacccc gccggtggtt tcggcgtagt tcgcggccag cacggcggcg
      841 cgggcgatgt cgccgccctg ctcgcccacc ggcgtcacgc agccccacaa aacatcgtcg
      901 accaaagccg tgtcgaggcc gttgcggtcg cgcacggcct ccagcacctg tttcgacagg
      961 ttgagcgccg tgaggtcgtg caggctgccg tccttcttgc ccttgccgcg cggcgtgcgc
     1021 gcatggtcgt agatgtaggc ggaattttca ggggtgggca tggattttca tcctccgggg
     1081 ctggcccgcg ctgcggcggg cttctgatgg ggaaatatct gcgctttgtt tacgcgagcg
     1141 tcaagataag cggaaaaaga ttctgaaatc ccggcggaaa cttgtgcatg gcgcgtctta
     1201 attatattac aattcttgac cctcacccgc aaagcggtta cgcgaggtca aacatctctg
     1261 gaaatacgac atggacacgt cacataccgc taagcttaac cccggcaaga cccggctggc
     1321 gcgcatcgaa accttcgccg tgccgccgcg ctggctgttc gtgcgcatcg agaccgacga
     1381 cggcgcggtc ggctggggcg aagcctcgct ggagggccat accgaggcca cgctagccgc
     1441 cttcgccgag atccgcgagc gcttcatcgg ccacgacgcc gggcgcatcg aggatatgtg
     1501 gcaggtgttt taccgcctcg gcttctatcg cggcgggccg gtgctgatgt cggccctgtc
     1561 cggcttcgat caggccctgt gggatctgaa ggcgcgccgc ctcggcgtgc cggtgtggca
     1621 attgctgggc ggtcaggtgc gcgagcgcgt gcaggtctat gcctggatcg gcggcgaccg
     1681 cccatccgag gtggtcgacg cgggccgcgc gcgcctgaag cagggcttca aggccgtcaa
     1741 gatgaacgcc agcgaggaca tgggctggct cgattcgccc agggtgatcg acggggtgtg
     1801 cgagcgcctg acctcggtgc aggccctcgg cctcgatgtc gggctggact tccacggccg
     1861 cgtgcataag ccgatggcgc ggcagatcgc gcacgcgctg gagcccctgc gcccgctgtt
     1921 catcgaggag ccgcttctgt ccgagcatcc cgaagcgatc gcgcagttcg ccgccgcgac
     1981 caccacgccg gtggcgctgg gcgagcggct ctattcgcgc tgggacttca agccgttctt
     2041 cgagcgcgcc tgcgtcgaca tcatccagcc cgacctcagc catgcgggcg gcatctccga
     2101 ggtccgccgc atcgccgcca tggccgaagc ctacgacgtc gccatcgcgc cgcattcgcc
     2161 gctggggccg ctggcgctgg gcgcctgcat gcagatcggg atcgcgacgc cgaaccacgc
     2221 cattcaggaa atgtcgctcg gcatccatta caacaccaac ggccacgacc ttctgttcta
     2281 catgaagaac ccggaagtct ttgacgtgaa ggacggctat gtcgaggccc tgaccggtcc
     2341 cggcctcggc gtcgagatcg acgaggacaa ggtgcgcgag atggcgaagg actgcccgcc
     2401 gtggcgcaac ccggcctggc gcgggccgga cggctacgtg cgcgaatggt gatgaaaaag
     2461 gccggcgtcc cgaacgccgg cctttgtttt tacagcccct cgaccttgcg tcccttgtcg
     2521 ccgggttgcg tcggcgtcgg cggcggcgcg gcttttttca ggctggcgtc ggcgaagccg
     2581 gtcagcccgg ccaccacctc ggtggtcgcc tggtcgaagc cctgcgagat cagcgtcatg
     2641 tcgttcctgc gcaccgacac gctggccgag atgtagcgct gcgccagcac cgaccggtcg
     2701 gacagccgca gcaggcgcgc gtcgacgccg atatagacgg tcggacggcc atgactgtag
     2761 ctggcctcga agcggcgcac gctgacgtcc agccgataat ccgccgccgt tggcccgcgc
     2821 gggaccagca tcaccgtctg gtccgagcgc gcgaaacctt ccgacaccgc cgccgcgaac
     2881 agcgccgagg ccgaggacgc ccagcgcgcc tgcgcgacat aggagacttc gttgtcctcg
     2941 gtggtcatca cctgatcgcc cgcgctggcg ggcgggaagc tgatcgcgcc cagcatcagc
     3001 ggaaccggcg ccgccgtcgt cgtggcggca gccgccgccg cgtccttatc cttgagcagg
     3061 tcgggatggt agccgaagcg ataaagctgc accggcttga ccttgggcag gacgttgacg
     3121 cagccgccca atcccagagt cagtcccacc gcgaaaaggg cgatgatgcg cgcgtgtttc
     3181 attggctgac ctttctttcc ttggcttccg gacgggcgat caggccgcgc gg
//
LOCUS       DASVLL010000183         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_333109,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000183 DASVLL010000000
VERSION     DASVLL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333109"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VF402_03020"
     CDS             <1..871
                     /locus_tag="VF402_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEX7799294.1"
                     /translation="KHKVRMAFGNGMRPEVWDHFKRRFKIPAIVEFYGSTEGNVSLFN
                     LDGQPGAVGRVPKILRSKFNVRLVRFDVETEMPIRGMNGLCQECKPGEIGEAIGIIGH
                     DVRHSYSGYADKAATQKKILTDVLKKGDAWFRTGDLMRQDKHGYLYFVDRIGDTFRWK
                     GENVSTSEVAEYCASAPGVEEAIVYGVPVANHDGKAGMVALITREGFDLAAFKAHVDS
                     HLPDFARPRFVRLLSEVETTGTFKYKKMDLVSEGFDLKRVRDPMFVCAHNGAYEPLTA
                     DLLGAIDAGLLRL"
     gene            930..2135
                     /gene="nhaA"
                     /locus_tag="VF402_03025"
     CDS             930..2135
                     /gene="nhaA"
                     /locus_tag="VF402_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX7799295.1"
                     /translation="MKTPFLIPSFTLAFLKTEAGSGFLLAACALLALLWANSPWAGAY
                     FGFIDHPIALQIGAWRVEESVLGWTKEGLMSVFFFVVGLEIKYEAMRGALSDKRKLAL
                     PVIAALGGMIAPALVCLAVNAALPHGDARGWSVPTATDIAFALAVLAMAGKGLPPALR
                     VFLLTLAIVDDLGAVVIIGVFYNNRFDPPYIAALIVLLGVMAGLKYRLRGKGLILAYA
                     VLFAAVWCVSLPAGVAPSLTAVAAAFCVGLEPSRPGEDGPLKRFMHALHPVNAYLILP
                     FFAFTAAGFSLKGLGAGLLLDPRCLGVLLGLFVGKQAGVFLACRLAIRLRLAHMPDGA
                     DVRQLYAVCALCGVGFTMSLYMAALAFRDGDPRALIAVKAGVIAGSLLAAATGAALLS
                     WRRRPDKTP"
     gene            2158..3525
                     /locus_tag="VF402_03030"
     CDS             2158..3525
                     /locus_tag="VF402_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX7799296.1"
                     /translation="MRSMSYRPAPLLSRSGLDLFDPVAAADFPQAVLRCRNVRAARGV
                     GLDALSDAGWINHFGRFAPLPDNQPQPLALRYHGHQFRHYNPDLGDGRGFLFAQMYDD
                     DHRLLDLGTKGSGRTPYSRGGDGRLTLKGGVREVLATAMLEALGVPTSKSFSLIETGE
                     ALTRGDEPSPTRSSVLVRLSHSHIRFGTFERLAFLNDADTMRGLVAFIREHYYPDAGN
                     AADLFDSIAANTARLAARWMAAGFVHGVLNTDNMNVTGESFDYGPYRFLPSCQPGFTA
                     AYFDHAGLYAYARQPDAALWNLGQLGRCLSLITDPEPLVASLNVFAGRYQSELEQAFC
                     ARLGVVPTGADEALIAAFFTLAYGLRFDGAFEGLIYDACTRRLGDSPRAGHYRGPEFD
                     AFKAALDAYEPMQRKHPRLLQPDPETLLYDEIEAIWARIAEADDWSLFDAKLAAIDVY
                     REALA"
     gene            complement(3522..3623)
                     /locus_tag="VF402_03035"
     CDS             complement(3522..3623)
                     /locus_tag="VF402_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7799297.1"
                     /translation="MRFPDESHWVSKPQNSLMWHRTVFDWLDKHLKP"
BASE COUNT          525 a         1291 c         1163 g          655 t
ORIGIN      
        1 gaagcacaag gtgcgcatgg ccttcggcaa cggcatgcgc ccggaggtgt gggaccattt
       61 caagcgccgc ttcaaaatcc cggccatcgt cgaattctac ggctcgaccg agggcaatgt
      121 ctccctgttc aacctcgacg gccagcccgg cgcagtcggg cgcgtgccca agatcctgcg
      181 cagcaagttc aacgtccgcc tcgtgcgctt cgacgtcgag accgagatgc cgatccgcgg
      241 catgaacggc ctgtgtcagg agtgcaagcc cggcgagatc ggcgaggcca tcggcatcat
      301 cggccacgac gtgcgccact cctattccgg ctacgccgac aaggcggcga cgcagaagaa
      361 gatcctcacc gacgtgctga aaaagggcga cgcctggttc cgcaccggcg acctgatgcg
      421 tcaggacaag cacggctatc tctatttcgt cgaccgcatc ggcgacacct tccgctggaa
      481 gggcgagaac gtctcgacct ccgaggtggc cgaatactgc gcctccgcgc cgggggtcga
      541 ggaggccatc gtctacggcg tgccggtcgc caaccacgac ggcaaggcgg gcatggtcgc
      601 gctgatcacg cgcgagggct tcgatctggc tgcgttcaag gcgcacgtcg attcgcacct
      661 gcccgacttc gcgcgcccgc gcttcgtgcg cctgctcagc gaggtcgaga cgaccggcac
      721 cttcaagtac aagaagatgg atctggtcag cgagggcttc gacctgaagc gcgtgcgcga
      781 tcccatgttc gtgtgcgccc acaacggcgc ctacgagccg ctgacggcgg acctgctggg
      841 cgccatcgac gccgggctgt tgcggctctg aaacgggcgg ggaccgaaaa cgcttaagct
      901 ttccggcggg caacggtaca ttccttggaa tgaagacccc gttcctgatc ccgtctttca
      961 ccctcgcttt tttaaaaacc gaggccggtt ccggcttcct gctggccgcc tgcgcgctgc
     1021 tggcgctgct gtgggccaat tcgccgtggg ccggcgccta tttcggcttc atcgaccacc
     1081 ctatcgccct acagatcggg gcgtggcgcg tcgaggaaag cgtgctgggc tggaccaagg
     1141 aggggctgat gtcggtcttc ttcttcgtcg tcgggcttga gatcaagtac gaggcgatgc
     1201 gcggcgccct gtcggacaaa cgtaagctgg ccctgccggt gatcgcggcg ctgggcggca
     1261 tgatcgcccc ggctctggtc tgtctggcgg tcaacgccgc cctgccgcac ggcgacgcgc
     1321 gcggctggtc cgtgccgacc gccaccgaca tcgccttcgc gctggccgtg ctggcgatgg
     1381 cgggcaaagg tttgccgccc gccttgcgcg tctttctgct caccctcgcc atcgtcgacg
     1441 atctcggcgc ggtcgtcatc atcggcgtgt tctacaacaa ccgtttcgac ccgccctata
     1501 tcgccgcgct catcgtcctg ctcggcgtca tggcggggtt gaaatacagg ctgcgcggca
     1561 agggcctgat cctcgcctac gccgtgctgt tcgccgccgt ctggtgcgtc agcctgcccg
     1621 ccggggtcgc gccgtccctg accgccgtgg ccgccgcctt ctgcgtcggc ctggagccgt
     1681 cgcgccccgg cgaggacggc ccgctcaagc gcttcatgca cgcgctgcac ccggtcaacg
     1741 cctatctgat cctgcccttc ttcgccttca cggcggcggg cttttcgctc aaggggctgg
     1801 gcgcgggcct gctgctggac ccgcgctgcc tcggcgtgct gctgggcctg ttcgtgggca
     1861 agcaggcggg cgtgttcctc gcctgccgtc tggcgatccg gctgcgcctc gcgcacatgc
     1921 ccgacggcgc cgacgtcagg cagctttacg ccgtctgcgc gctgtgcggc gtcggcttca
     1981 ccatgagcct ctatatggcg gcgctggcct tccgcgacgg cgatccgcgc gcgctgatcg
     2041 ccgtcaaggc cggggtgatc gccggttcgc tgctggccgc cgccacgggc gccgcgctgc
     2101 tgtcgtggcg gcgtcggccc gacaagacgc cttgacttgt ccgccgcgcg cgccgacatg
     2161 cgcagcatgt cctatcgccc cgccccgctt ctgagccgga gcggcctcga cctgttcgat
     2221 ccggtcgccg ccgccgattt tccgcaggcc gtcctgcgct gccgcaatgt ccgcgccgcg
     2281 cgcggcgtcg ggctggacgc cttgagcgac gccggctgga tcaatcattt cggccgcttc
     2341 gcgcccctgc ccgacaacca gccgcagccg ctggccctgc gctatcacgg ccatcagttc
     2401 cgccactaca atcccgacct cggcgacgga cgcggcttcc tgttcgcgca gatgtacgac
     2461 gacgaccacc gcctgctcga cctcggcacg aaaggctccg gccggacgcc gtattcgcgc
     2521 ggcggcgacg ggcggctgac gctgaaaggc ggcgtgcgcg aggttctggc caccgccatg
     2581 ctggaggcgc tgggcgtgcc gacctcgaaa tcgttttcgc tgatcgagac gggcgaggcg
     2641 ctgacgcgcg gcgacgagcc gtcgccgacg cgctcgtccg tgctggtccg gctcagccac
     2701 agccatatcc gcttcggtac gttcgagcgg ctggccttcc tcaacgacgc ggacacgatg
     2761 cgcggcctcg tcgccttcat ccgcgagcat tactatcccg acgccgggaa cgccgccgac
     2821 ctgttcgact cgatcgcagc caataccgcc cggttggccg cgcgctggat ggcggcgggc
     2881 ttcgtgcatg gcgtgctgaa caccgacaat atgaacgtca ccggcgaaag cttcgattac
     2941 ggcccttacc gtttcctgcc gtcctgtcag cccggcttca ccgccgccta tttcgaccat
     3001 gccgggcttt acgcctatgc gcgccagccc gacgcggcgt tgtggaatct cggccagttg
     3061 gggcgctgcc tgagcctgat cacggacccg gagccgctgg tcgccagcct caacgtcttc
     3121 gccggacgct atcaaagcga gctggagcag gcgttctgcg cgcggctcgg cgttgttcct
     3181 accggcgccg acgaggcgct catcgccgcc tttttcacgc tggcctatgg cttgcgcttc
     3241 gacggcgcgt tcgagggcct gatctacgac gcctgcacgc gtcgcctcgg cgacagcccg
     3301 cgcgccgggc actatcgcgg gccggagttc gacgccttca aggccgcgct cgacgcctat
     3361 gaaccgatgc aacgcaagca tccgcgcctg ctgcaacccg atcccgaaac cctgctctac
     3421 gacgagatcg aagccatctg ggcgcgcatc gccgaggccg acgactggtc cctgttcgac
     3481 gccaagctgg ccgccatcga cgtctatcgc gaggcgctgg cttaaggttt caggtgtttg
     3541 tccagccagt cgaacaccgt cctgtgccac atcagcgaat tctgcggctt gctcacccag
     3601 tggctttcat cgggaaaacg caggaattcc gatt
//
LOCUS       DASVLL010000184         5401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_494965,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000184 DASVLL010000000
VERSION     DASVLL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494965"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VF402_03040"
     CDS             complement(<1..615)
                     /locus_tag="VF402_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7799298.1"
                     /translation="MKKSLAFAALLLTAAPVCVPACAQARTRVFVSMARVDPFLSLIR
                     DSLQAEAAKNPDLDMTIAVANDQVDTQLSQVKAAIADHTDVLVVLSVDDNAARQLAAA
                     SAASKTPLVYLNVLPPIDRFTTPTSIVASNDLVAGRLQMRLLADKIGNKGNVVILRGA
                     DSHPAARDRTAGVKEILAQRPDIHVVGDVSCDWKRDEAEKQVTAW"
     gene            complement(631..2316)
                     /locus_tag="VF402_03045"
     CDS             complement(631..2316)
                     /locus_tag="VF402_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7799299.1"
                     /translation="MLRFVQSIRGKIALAFGFLMIVSLATGAFSIAKLGEVSKISARL
                     SDETAAVSVLGDLARTSQTLSVGSLLEHFAQDDTARQTYAQHGAEARSAFAKSWSQYS
                     DMVSGPVESAKAATLRKAWQHFLAVQEEVADLDKAGLHDEAAGVLMNDLQQEGAAFFA
                     AVQDVQTFRQTQADAAKAQAKRVNASARFWILTAMGSLTAVCVLTGVTLTLSISRPLG
                     RLTEAMLKLAAQDMRANIPDTRRRDEIGGMARSLQVFREKMIEAETLRSGQAEAEAQM
                     QARRRAEIAQMADDFETTVGGIVTLVSAAASQLQGSAQSLSAAAEQTSMQSAAVENGA
                     RQAADNVRSVAAAIEELAASARQVGSDVARSSQISNAAVQQAGRTRASMDALGDDAQQ
                     VGSVVGVISDIASQTNLLALNATIEAARAGEAGKGFAVVASEVKGLADQTAKSTTTIN
                     VSIAKMQASTQQAAGEISGIEQTISEVDEIAAAIAGAVSQQEATTSEIARNIHEVSQN
                     TSEVTDNINGVTTAARESSRGAMQVLSAASDLAQQSEALKGEVDRFLSRVRAA"
     gene            complement(2419..3600)
                     /locus_tag="VF402_03050"
     CDS             complement(2419..3600)
                     /locus_tag="VF402_03050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7799300.1"
                     /translation="MPLLMRKPETAATKPGDASRPADPQPDQPPAPPLKDWPSLDALG
                     ERMVKLRFTPGLSLSVMHDGVMLYSKGFGSGDLQTGARVTPRTGFRIASVTKQFTAAA
                     ILKLQEQGKLSVHDNLARFMPEFPQADQVTLAQMMSHTSGMNDYLNHQAQSVLVAAQT
                     RDYTSPELLRIISAVHPVYSIRPGQRWLYSNSAFSLLGIVVERVSGQNLADFYQTHLF
                     APAGLRQTAMDERCSAFDGCDGYRPNYRIAAGFDPILPVSPTFAGGAGGIRSNTEDLT
                     RWHDALFGGKVLKAASFAQMITPMLLKDGSPAWEYVGPEKLNYGFGQGIGHLDGRAFI
                     AHGGRLNGYTSHLRSLVHERLTVAVLYNSDGTGVPGFTGAQRALRIEAMKLGMKEIGI
                     A"
     gene            3880..4317
                     /gene="mutT"
                     /locus_tag="VF402_03055"
     CDS             3880..4317
                     /gene="mutT"
                     /locus_tag="VF402_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEX7799301.1"
                     /translation="MGISRVKTIGDLAVKIVLVVAAALVDSDGRVLIAQRPQGKQLAG
                     LWEFPGGKVEPGETPEDALIRELAEELGVSVKKACLAPFVFASHTYEDFHLLMPLYLI
                     RRWEGEPERREHAALKWVRPNAMRDYPMPPADAPLVAYLQDFI"
     gene            complement(4321..4587)
                     /locus_tag="VF402_03060"
     CDS             complement(4321..4587)
                     /locus_tag="VF402_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799302.1"
                     /translation="MSLTDVDLPALKLLMRRILEARHAGDRTEAFSLFRAQLLRHIDT
                     GEDAKRQPVRLYLDRLSRLPCGSEPWLDIFRALQRTLESHALAA"
     gene            4787..5356
                     /locus_tag="VF402_03065"
     CDS             4787..5356
                     /locus_tag="VF402_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX7799303.1"
                     /translation="MPLTVRIEGFPAGAAIPERFAHCAKNGSGGVKPGGNLNPAIQWE
                     NAPFDTQSFALIVVDRDVPADFGPANKPGQSIAADAPRRSFYHWLVVDIPPAIARIEP
                     GETPDGQIGRNDFGDGAKGANGYDGPCPPFNDERLHTYRFMVYALDVTTLNLPDGYTG
                     EQAERALAGHVLAEGEVTGLYTTNPKLTA"
BASE COUNT          843 a         1737 c         1896 g          925 t
ORIGIN      
        1 ccaggccgtc acctgctttt cggcctcgtc gcgcttccag tcgcacgaga cgtcgccgac
       61 gacgtggatg tccgggcgct gggccagaat ctccttcacg cccgccgtgc ggtcgcgcgc
      121 cgccgggtgg ctgtcggcgc cgcgcaggat cacgacattg cccttgttgc cgatcttgtc
      181 ggccagcagc cgcatctgca ggcgcccggc cacgaggtcg ttcgacgcca cgatcgaggt
      241 cggcgtggtg aagcggtcga tcggcggcag cacgttgaga tagacgagcg gcgtcttcga
      301 ggccgccgac gccgccgcca gttgccgggc cgcgttgtcg tcgaccgaca gcacgaccag
      361 cacgtcggtg tgatcggcga tggcggcctt gacctggctg agctgggtgt cgacctgatc
      421 gttggcgacc gcgatggtca tgtcgagatc gggattcttc gccgcctcgg cctgcaacga
      481 atcgcggatc agcgacagga acgggtcgac gcgcgccatc gagacgaaca ccctggtcct
      541 ggcctgagcg caggcgggga cgcagacggg cgcggcggtc agaagcaggg cggcgaacgc
      601 aagggacttt ttcatggctg tctccttcgg tcaggcggcg cgcacgcgcg acaggaagcg
      661 gtcgacctcg cccttgagcg cctcggattg ctgcgccaga tcgctggcgg cgctcagcac
      721 ctgcatggcg ccccggctcg attcgcgcgc ggcggtggtc acgccgttga tattgtccgt
      781 cacctcggac gtgttctgcg acacctcgtg gatgttgcgc gcgatctcgg aggtggtcgc
      841 ctcctgctgg ctgaccgcgc cggcgatggc ggcggcgatt tcgtcgacct cggaaatggt
      901 ctgttcgatg ccggagatct cgcccgccgc ctgctgggtc gaagcctgca ttttcgcgat
      961 ggagacattg atggtggtgg tcgatttggc ggtctggtcg gccagcccct tgacctcgga
     1021 cgcgacgacg gcgaagccct tgcccgcctc gcccgcgcgc gcggcctcga tggtggcgtt
     1081 gagcgccagc aggttggtct gcgaggcgat gtcgctgatc acgccgacga ccgagccgac
     1141 ctgctgggcg tcgtcgccca gcgcgtccat gctggcgcgg gtgcggcccg cctgctgcac
     1201 ggcggcgttg ctgatctggc tggagcgcgc cacgtccgag ccgacctgac gcgccgaggc
     1261 ggccagttcc tcgatggcgg cggcgaccga gcgcacattg tcggcggcct gacgcgcgcc
     1321 gttctcgacg gcggcggact gcatcgaggt ctgctcggcg gcggcggaca gggactgcgc
     1381 cgagccctgc aactggctgg cggcggcgga gacgagggtg acgatgccgc ccacggtcgt
     1441 ctcgaaatcg tcggccatct gcgcgatttc ggcgcggcgg cgggcctgca tctgcgcctc
     1501 ggcctcggcc tggccggagc gcagggtttc ggcctcgatc atcttttcgc ggaagacctg
     1561 caacgagcgc gccatgccgc cgatctcgtc ccggcgccgg gtgtcgggaa tgttggcgcg
     1621 catgtcctgc gcggccagtt tcagcatggc ctccgtcagg cggcccagcg ggcgcgaaat
     1681 gctcagggtc agggtcacgc cggtcagcac gcacaccgcc gtcagcgagc ccatcgcggt
     1741 cagaatccag aagcgcgccg aggcgttgac gcgcttcgcc tgcgccttgg cggcgtcggc
     1801 ctgcgtctgg cggaaggtct gcacgtcctg cacggcggcg aagaaggcgg cgccttcctg
     1861 ttgcaggtcg ttcatcagca cgcccgcggc ttcgtcgtgc aggcccgcct tgtcgaggtc
     1921 ggcgacctct tcctgcacgg ccaggaagtg ctgccacgcc ttgcgcaacg tcgcggcctt
     1981 ggcgctttcc accggcccgg acaccatgtc ggaatactgg ctccagctct tggcgaaggc
     2041 gctgcgcgcc tcggcgccgt gctgggcgta ggtctggcgg gccgtatcgt cctgcgcgaa
     2101 atgttcgagc agcgagccga cgctcagcgt ctggctggtc cgcgccagat cgcccagcac
     2161 cgacacggcg gcggtctcgt ccgacaggcg ggcgctgatt ttcgacacct cgcccagctt
     2221 ggcgatcgag aaggccccgg tcgccagcga tacgatcatc agaaagccga acgccagggc
     2281 gatcttgccc cgaatcgact gcacgaacct cagcatggcc cttgcctccc aagccgcttc
     2341 atcttgtttt tggagggcag gttagggggg taaacagaaa ttttctgtta accgcgatag
     2401 cgctaccata gcgtgtaatc aggcgatgcc gatctctttc atgcccagtt tcatcgcctc
     2461 gatgcgcagc gcgcgctgcg cgccggtgaa gccgggcacg ccggttccgt cgctgttgta
     2521 cagaacggcg acggtcaggc gctcatgcac gaggctgcgc aggtggctgg tgtagccgtt
     2581 gagccgcccg ccgtgggcga tgaaggcgcg gccgtcgaga tggccgatgc cctgaccgaa
     2641 gccgtagttg agcttttccg gtccgacata ttcccaggcg ggcgagccgt ccttcagcag
     2701 catcggcgtg atcatctgcg cgaagctggc ggctttcagc accttgccgc cgaacagggc
     2761 gtcgtgccag cgcgtcagat cctcggtgtt ggagcggatg ccgcccgcgc cgcccgcgaa
     2821 ggtcggcgac accggcagga tcggatcgaa acccgccgcg atgcggtagt tgggccggta
     2881 gccgtcgcag ccgtcgaagg ccgaacagcg ttcgtccatc gcggtctggc gcaggcccgc
     2941 cggggcgaac aggtgagtct ggtagaagtc ggccaggttc tggcccgaaa cgcgctcgac
     3001 cacgatgccg agcaggctga aggcgctgtt ggaatagagc cagcgctggc cggggcggat
     3061 gctgtagacc ggatgcacgg cgctgatgat ccgcagcagt tccggcgagg tgtagtcgcg
     3121 cgtctgggcc gccaccagca cgctttgcgc ctgatggttg agatagtcgt tcatgccgga
     3181 ggtgtggctc atcatctgcg ccagagtcac ctgatcggcc tgcggaaatt ccggcatgaa
     3241 gcgcgccagg ttgtcatgca cgctcagctt gccctgctcc tgcaatttga ggatggcggc
     3301 ggcggtgaac tgcttggtga ccgaggcgat gcggaagccg gtccgcggcg tgaccctggc
     3361 gccggtttgc aggtcgccgg agccgaagcc cttggaatag agcatcacgc cgtcgtgcat
     3421 cacgctcagg ctcaggcccg gcgtgaagcg cagcttcacc atccgctcgc ccagcgcgtc
     3481 gaggctgggc cagtccttca gcggcggcgc gggcggttgg tcgggttgcg gatcggcggg
     3541 ccgggacgcg tcgccgggct tcgtcgccgc cgtttccggc ttgcgcatca gcagcggcac
     3601 ggtgagggcg cccgccgcca gaccggcggc gataaagccg agcgccgcgc ggcgggacgg
     3661 gcgttcggtc ggatcgaagg gcgtgagatc ggtcttggga tatcaggcgg gtttcaggct
     3721 acgcgggtta caagataaca gataacttgc catcatcggc gcggcgcggc aagcgctgcg
     3781 gcgatgcggg gatatggaat tgaagacgtc cagagcgcgc cggaacggtt aagaaagcgt
     3841 tgccaattgt ggaaattcga tggcggcgcg gtcacgaata tgggaataag ccgcgtgaag
     3901 accatcggag acttagctgt gaagatcgtt ctcgtcgtcg ccgccgccct tgtcgacagc
     3961 gacgggcgcg tgctgatcgc gcagcgcccg cagggcaagc aactggcggg cctgtgggag
     4021 tttccgggcg gcaaggtcga gccgggcgaa acgccggagg acgccctgat ccgcgaactg
     4081 gcggaggagc tgggcgtttc ggtcaaaaaa gcctgtctgg cgccgttcgt cttcgcctcg
     4141 cacacctatg aggattttca cctgctgatg ccgctgtacc tgatccggcg ctgggaaggc
     4201 gagccggagc ggcgcgagca cgcggcgctg aaatgggtgc ggcccaacgc catgcgcgac
     4261 tatccgatgc cgcccgccga cgcgccgctg gtggcctatt tgcaggattt tatctgagcc
     4321 ttacgccgcc agcgcgtgag attccagcgt cctttgcagg gcgcggaaga tgtcgagcca
     4381 cggctctgaa ccgcacggca ggcgcgacag gcggtcgaga tagaggcgca ccggctggcg
     4441 cttcgcatcc tcgccggtat cgatatgacg cagcagttgc gcgcggaaca gggagaaggc
     4501 ttcggtgcgg tcgcccgcgt ggcgcgcttc gaggatgcgg cgcatcagca gcttcagggc
     4561 gggcaggtcg acgtccgtca ggctcatggc gatctccatt cctaaatatt tgaaaagtcg
     4621 cggaaatcta tcaggcggtc cataagattg ccgttgcgtc gcccgcgcgg ggatatcagg
     4681 aaacgataag gacggagcgg ggcggacgat cgggccggcg ccgcattgac tccttatcgt
     4741 ttccgcgcga agcctgtcct gtcgacaggt agattgggag aggatcatgc cgcttaccgt
     4801 tcgcatcgag ggctttccgg cgggcgccgc cattcccgaa cgcttcgccc actgcgcgaa
     4861 gaacggttcc ggcggcgtga agcccggcgg caatctcaat cccgcgatcc agtgggagaa
     4921 cgcgccgttc gacacgcaat ccttcgcgct gatcgtggtc gaccgcgacg tgcccgccga
     4981 tttcggcccg gcgaacaagc ccggtcaatc gatcgccgcc gatgcgccgc gccgcagctt
     5041 ctatcactgg ctggtcgtcg acattccgcc cgccatcgcg cgcatcgagc cgggcgagac
     5101 gccggacggc cagatcggcc gtaacgattt cggcgacggc gcaaagggcg ccaacggcta
     5161 tgacggcccg tgcccgccgt tcaacgacga acggctgcac acctatcgct tcatggtcta
     5221 tgcgctggac gtgacgacgc tgaacctgcc ggacggctat accggcgagc aggccgaacg
     5281 cgcgctggcc ggacacgtgc tggccgaggg cgaagtcacc gggctttaca ccaccaaccc
     5341 gaagctgacg gcgtaggcgc tgtttccccc tctcccctcc acgaggggag aggacgaggc
     5401 g
//
LOCUS       DASVLL010000185         9462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_118227,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000185 DASVLL010000000
VERSION     DASVLL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118227"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VF402_03070"
     CDS             <1..1064
                     /locus_tag="VF402_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7799304.1"
                     /translation="LKPGCGWLPGDWMPQEELCRALRALARRPDANLVDYDLPAGHAG
                     LRQHLSRRLADKDIDAPPSQIVLMDSATQAIELLCRFLLKPGDTVLVDDPCFFNFLAK
                     LRAHRVDILGVPMTPGGPDVAALADILSAHRPRLYLTNSALHNPTGASLSPLVAHRVL
                     KLAEAHDLIIIEDDIYSDFELEPGPRLAAFDGFERVVYIGGFSKTLSASLRLGFIAAR
                     ADWIEPLTDLKIATVFSSQGFAADVAHQVLSSGGYRRHVESLRQKLAVRRAPTAGRLA
                     DLGVTPWLTPQAGMFLWCELPEGMDASDLARRALDERLVLAPGNAFSVGQRAARCLRF
                     NIAQSQDARLYSFLRKHVC"
     gene            1186..3387
                     /locus_tag="VF402_03075"
     CDS             1186..3387
                     /locus_tag="VF402_03075"
                     /inference="COORDINATES: protein motif:HMM:NF026034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HEX7799305.1"
                     /translation="MKPTQALALVAALTFCAGPLAARAQTPPPVTVTDNGASWTLDNG
                     VVKVTVQKDTGYMPSLIYKGKELMSANKGGSWEHTPAGAPHLTNSVTIDPSTNGGARA
                     EVSVKGVTDGVYTLSPHAPGGGAQADMEIRYALGRGDSGVYVYAIYDHPASYPAGGTG
                     AEDRFITRLSHDFDWITVDKDRNMLEASPADWGAGVVVHAKEQRILSTGVYKNSVEHK
                     YSYSGMQYRQPAYGWSSTKDHIGVWFINPTVEYLSGGPTRIDLDAHFDSDNDPDPIIL
                     DYWHSGHYAGANTPLKAGEAWRKVIGPIFVYVNALDRPQPTTDAELKTLAETAGDPTV
                     PQSWTANADALYADALAQAKKENAAWPYGWVKAEGYTPADQRGTVTGRFVLNDPLAPA
                     GTPKTFPDLVVGLTQAGDPDDWAPQHAMPGRNAAAFMAQYKALHTGPDAYRHDARFYQ
                     FFNDGKPDGEFAITKVRPGTYTLHAYSDGILGEYLKADVTVEPGKTLDLGALDWTPER
                     HGRQLWQIGYPNRSASEFFKGDQAWLWGWNLRYALLFPHDVTYTVGKSDPAKDWFFEE
                     VPHATDLSFVNPAAKDPANQKFGWVKAESLAQYPQSDHTGPWRVYGNGRATVWKVRFK
                     LPKAEQGTAALRVALAGEDGMDGQLPVALNGQSLGAVGDGSTPDNARMIQTNAIRYNT
                     DRGLWQERTLHFDAAKLKKGWNEMTFTVPAGSLQSGVVWDYLRLEVDEAGK"
     gene            complement(3888..4237)
                     /gene="ssrA"
                     /locus_tag="VF402_03080"
     tmRNA           complement(3888..4237)
                     /gene="ssrA"
                     /locus_tag="VF402_03080"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            complement(4236..5081)
                     /locus_tag="VF402_03085"
     CDS             complement(4236..5081)
                     /locus_tag="VF402_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX7799306.1"
                     /translation="MSIRARIMALVACFALMALVVTGLGLATIADYNRMMKDYGRAYE
                     NAWRGERLNHLVSNVVMESRGVYIARNREEMDTFVTGLATNLDEIDDLIKTWKADSSP
                     QELRGLQPILNDTQTFLTVRRDLIRLATDGDQKGAETRGVASRDDRIAFQNEIERQVD
                     DLRAKLASAKAKADSYNRRRAGEFLATALVGIALMLGLSLWIVAHFITRPLHDVASAI
                     IKTSKGDYDTPIDDSLDAQDEVSHVWQALAVLKARAQEAERLAAAKAEAEHREEMKLR
                     EILLD"
     gene            complement(5170..6552)
                     /locus_tag="VF402_03090"
     CDS             complement(5170..6552)
                     /locus_tag="VF402_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7799307.1"
                     /translation="MKTLALTSLALTSLALTSLALSACTLAPKYVRPALPVAQDWPVA
                     DAAQGRNAGDLPWKQVFLDARLQHVIDAALLNNRDLRVAALNIETARASYLMTRSGLL
                     PALNGNLGETRTHQAGVDSDLYSAALAVPAWELDLFGKARSQAQAAKESFLASDETRR
                     ATQISLISETAQAWFTLAADQDLLRLARETLRTRQEGYDLTQRKFDLGAAAETDLRAL
                     EILTEQARADAAQYETQVQQDKDALNLLAGTQLSEADLPRTLPDGAVLSDLPAGLPSD
                     VLTRRPDVLAAEHAIKAADGDIGAARAAFFPSVSLTGSTGSASDSLNDLFTSGTGAWS
                     FAPSLSLPIFRGGYNLANLKGARASQQIAVASYEKTVQAAFRDVADALAVRAGIETRL
                     TATGKAANAAGAALTLGQARYDKGVDSYLNLLDAQRTAYSAQQALIAVQLARAANLAG
                     LYAALGGGVS"
     gene            complement(6575..>9462)
                     /locus_tag="VF402_03095"
     CDS             complement(6575..>9462)
                     /locus_tag="VF402_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7799308.1"
                     /translation="DIAQVQVQNKLQTVMALLPQSVQAQGVSVVKSTSNFLLVVGVYA
                     DDGKTTQTDISDYIGSSIKDQLSRVDGVGDVQLFGAQYAMRIWLDPQKLASYNLMPSD
                     VTAAIQAQNTQVSAGELGGLPAVPGTMLNATITAQSRLTTPEQFRNIIVKTQSDGAKV
                     YLSDVARVELGAESYASSATYNGHPASGIAVKLTPGGNALNTAKAVKARMDELSRSFP
                     AGMHYVVPYDTTPFISLSIEEVVKTLVEAVVLVFLVMYVFLQNWRATLIPTIAVPVVL
                     LGTFGILAAVGYSINTLTLFGLVLAIGLLVDDAIVVIENVERIMSEEGLPPRVATRKS
                     MGEITGALIGIALVLAAVFVPMAFFTGSQGMIYRQFSVTLVSAMALSVTVALILTPAL
                     CATLLKPVKKGEGHIQTGLAGWFNHRFDAASNRYQGTVRKILTQPARWMIGFALIIGA
                     VALLFTRLPTAFLPEEDQGIVFTLIQLPAGATQERTQKVVDDVRRYFQKDPAVSAVFG
                     VTGFSFGGQGQNMAMAFVRLKDFDKRHASKDKAQAVAMRGIMHFAGYPNATIYAIVPP
                     AISSLGASSGFTMMLEDQGGVGHDKLIQARNQLLGMAAKDPLLSAVRPNGQEDTAQLD
                     VDIDQAKAGALNLTLSDVNSVLSTAWGGAYVNDFIDRGRVKKVYVQADAPFRMKSEDL
                     DRWFVRNSGNQMVPLSSIATEKWSFGAPSLTRYNGLPAVELDGAPAPGKSSGAAMAEM
                     EKLAKQLPPGVGYEWTGISLQEQQSGNQASALYALSIAVVFLLLAALYESWSIPLAVI
                     MVIPLGVLGALLAMSFRGLNNDVYFQVGLLTIIGLSAKNAILIVEFAKSLHEEGRTLT
                     EATLEAVRIRLRPIIMTSLAFTFGVLPLAISNGAGSGAQHAIGTGLIGGILSATFLAI
                     FFVPLFFVLVQKLFKADEKHVLKLQRMRDDAELERTSGGDDTPPAAH"
BASE COUNT         1593 a         3049 c         3207 g         1613 t
ORIGIN      
        1 atctcaagcc cggctgcggc tggctgcccg gcgactggat gccgcaggag gagctgtgcc
       61 gcgcgctgcg cgccctggcg cggcgcccgg acgccaatct ggtcgattac gacctgcccg
      121 cgggccatgc gggcctgcgg caacacctgt cgcgccgtct ggcggacaag gatatcgacg
      181 cgccgccgtc gcagatcgtg ctgatggatt cggcgaccca ggccatcgag cttctgtgcc
      241 gcttcctgct caagcccggc gatacggtgc tggtcgacga tccgtgcttt ttcaattttc
      301 tggccaagct gcgcgcccat cgggtcgaca tcctcggcgt gccgatgacg cccggcggcc
      361 ccgacgtcgc cgcgctggcc gatatcctga gcgcgcacag gccgcgcctt tacctgacca
      421 attcggccct gcacaatccg accggcgcca gcctgtcgcc gctggtcgcg caccgggtgc
      481 tgaaactggc cgaggcgcac gacctgatca ttatcgagga cgacatctac agcgatttcg
      541 aactggagcc gggaccgcgc cttgcggcct tcgacggctt cgagcgcgtc gtctatatcg
      601 gcggtttctc caaaaccctg tcggcctcgc tgcggctcgg cttcatcgcg gcgcgcgccg
      661 actggatcga gccgctgacc gatctgaaga tcgccaccgt cttctccagc cagggcttcg
      721 ccgccgacgt cgcgcatcag gttctgagca gcggcggtta tcgccgtcat gtcgaaagcc
      781 tgcgtcagaa gctggcggtg cggcgcgcgc cgaccgccgg gcgtctggcc gatctggggg
      841 tgacgccgtg gctgacgccg caggcgggca tgttcctgtg gtgcgagctg cccgaaggca
      901 tggacgcctc cgatctggcg cgccgcgcgc ttgacgagcg gctggtgctg gcgcccggca
      961 acgccttcag cgtcggccag cgcgcggccc gctgcctgcg cttcaacatc gcccagtcgc
     1021 aggacgcgcg cctgtacagc ttcttgcgca aacatgtttg ctgatgaagt atattgcgca
     1081 taagtgacaa aatctgtgac aatgcgcgca ggatttgtca tgcttcggcg tgaaagaccg
     1141 gatgcgggcg gcaagacccg cgccgtcagg ctcccggagg catgtatgaa accgactcag
     1201 gctctcgcgc tcgttgcggc gctgaccttt tgcgccggcc cgctggcggc gcgcgcgcaa
     1261 accccgccgc cggtcaccgt gaccgacaac ggcgcgtcat ggacgctgga caacggcgtg
     1321 gtgaaggtga cggtgcagaa ggacaccggc tacatgccct ccttgatcta caagggcaag
     1381 gagctgatgt cggccaataa gggcggctcg tgggagcata cgcccgcggg cgcgccgcac
     1441 ctgacgaaca gcgtcaccat cgatccgtcg accaacggcg gggcgcgcgc cgaggtgtcg
     1501 gtcaagggcg tgaccgacgg cgtctatacg ctcagcccgc acgcgccggg cggcggcgcg
     1561 caggccgaca tggagatccg ctacgcgctg gggcgcggcg attccggcgt ctatgtctat
     1621 gcgatctacg accatccggc cagctatccg gcgggcggca ccggggcgga ggaccgcttc
     1681 atcacccggc tgagccacga tttcgactgg atcacggtcg acaaggaccg caacatgctg
     1741 gaggcgtcgc ccgccgactg gggcgcgggc gtcgtcgtcc acgccaagga gcagcgcatc
     1801 ctgtccaccg gggtctataa gaactcggtc gagcataagt acagctattc gggcatgcag
     1861 taccgccagc ccgcctatgg ctggtccagc acaaaagatc atatcggcgt ctggttcatc
     1921 aatccgaccg tcgagtatct gagcggcggg ccgacgcgca tcgatctcga cgcgcatttc
     1981 gattccgaca acgatcccga tccgatcatt ctcgattact ggcattccgg ccattatgcg
     2041 ggcgccaata cgccgctcaa ggcgggcgag gcgtggcgta aggtgatcgg gccgatcttc
     2101 gtttacgtca acgcgctgga caggccgcag ccgaccacgg acgccgagct gaagacgctg
     2161 gccgaaacgg cgggcgatcc caccgtgccg caaagctgga ccgccaacgc cgacgccctc
     2221 tatgccgacg cgctggcgca ggcgaagaag gaaaacgccg cgtggcccta tggctgggtc
     2281 aaggccgaag gctatacgcc cgccgatcag cgcgggaccg tcaccgggcg cttcgtgctg
     2341 aacgatcctt tggccccggc ggggacgccg aagacctttc ccgatctcgt cgtcggcctg
     2401 actcaggcgg gcgatcccga cgactgggcg ccgcagcatg ccatgccggg ccgcaacgcc
     2461 gccgccttca tggcgcagta caaggcgctg cacaccgggc cggacgccta ccgccacgat
     2521 gcgcgcttct atcagttctt caacgacggc aagccggacg gcgaattcgc gatcaccaag
     2581 gtccggcccg gaacctatac gctgcacgcc tattctgacg gaatcctcgg cgaatatctc
     2641 aaggccgacg tcacggtcga gccgggcaag acgctggatc tgggcgcgct cgactggacg
     2701 ccggagcggc atggccgcca gttgtggcag atcggctacc ccaaccgctc ggccagcgag
     2761 ttcttcaagg gcgatcaggc gtggctgtgg ggctggaacc tgcgttacgc gctgctgttc
     2821 ccgcatgacg tgacctatac ggtcggcaaa agcgatccgg cgaaggactg gttcttcgag
     2881 gaagtgccgc acgcgaccga tttgtccttc gtcaatccgg cggcgaaaga cccggccaac
     2941 cagaaattcg gctgggtgaa ggccgaatcg ctggcgcagt atccgcagag cgaccacacc
     3001 ggtccgtggc gcgtgtacgg caatggccgc gcaaccgtct ggaaggtcag gttcaaactg
     3061 ccgaaagccg aacaggggac ggcggccctg cgcgtggcgc tggcgggcga ggacggcatg
     3121 gacggccagt tgccggtggc gctgaatggt cagagcctcg gcgcggtggg cgacggctcg
     3181 acgccggaca atgcgcgcat gatccagacc aacgccatcc gctacaacac cgaccggggg
     3241 ctgtggcagg agcggaccct gcatttcgac gcggcgaagt tgaagaaggg ctggaacgag
     3301 atgaccttca ccgtcccggc gggcagcctg caaagcgggg tggtgtggga ttatctgcgg
     3361 ctggaggtcg atgaggcggg gaaataggct gattgacggt ccccctttcg tcattggctg
     3421 cgccaacgcc gcctctcccg ttcacgcagg gcgcccgccc atagccgcac agcggcgaac
     3481 cttgcgactt caattaccgg cgatagcggt agcgtgaaag ggataagaac atctgtctta
     3541 tctgtggata ccccttcctg ctgtcgtcag agcgcgcgcg aaggtcggtt ttttgatcca
     3601 cagataagac agatgttcat aatcaccgca cgctaccgct atcgccaaag tccgtttcaa
     3661 gcgatggaaa ggtggagttg tcacatccac gccatatgcg atagccctgt ctccgcgagg
     3721 gggagggacc gcgaagcggt gggaggcgcc tctgcctatg ggtggaaaag tgacaggctt
     3781 ctgttgccag gcgcctgcca tttttcatca gatttcggag aggcgcgcgc atggttgcaa
     3841 gtggagaggc ttctgttgcc aggcgcctca gcataacttt ttaaggaagt gacaggcttc
     3901 tgttgccagg cgcctgtcgg gccccgcctg acgaggctaa tcgtcaggag tttggttcgg
     3961 aattactccg ctgcattacg cagcaagagc gtactccgga gcgaagttat cgttcgcagt
     4021 taaaaaatga ggcgatcaca gagcctcacg ctgagggaaa gcttcacctt tacacgcctg
     4081 tcgatgctga tcggccccat aacgtcccgg aagggaatgg tggagccgcc gggaatcgca
     4141 cccgggtcca gttcgcttat tacgtgggcg tttatcccca tagtccggtc gcccggacgc
     4201 gcctaatata ggcgctggcg cgggcgaggg gaagcctagt cgagcagaat ttcgcgcagc
     4261 ttcatttcct cgcggtgttc ggcctcggcc ttggcggcgg ccagccgctc ggcctcctgc
     4321 gcgcgcgcct tgagcacggc cagcgcctgc cagacgtgcg acacctcgtc ctgcgcgtcg
     4381 agcgaatcgt cgatcggcgt gtcgtagtcg cctttcgagg tcttgatgat ggccgaggcg
     4441 acgtcgtgca gcggccgggt gatgaaatgc gcgacgatcc acagcgacag gcccagcatc
     4501 agcgcgatgc cgaccagcgc cgtcgccagg aattcgccgg cgcggcggcg gttgtagctg
     4561 tcggccttcg ccttggcgct ggccagcttg gcgcgcaggt cgtcgacctg acgctcgatc
     4621 tcgttctgga aggcgatgcg gtcgtcgcgg ctggcgacgc cgcgcgtttc ggcccccttc
     4681 tggtcgccgt ccgtcgccag ccggatcagg tcgcggcgca cggtgaggaa ggtctgcgtg
     4741 tcgttcagga tcggttgcag gccgcgcagt tcctgcggcg aggaatcggc cttccacgtc
     4801 ttgatcaggt cgtcgatctc gtcgagattg gtggccaggc cggtgacgaa ggtgtccatc
     4861 tcctcgcggt tgcgcgcgat atagacgccg cgcgattcca tgaccacgtt ggagacgagg
     4921 tggttcagcc gctcgccgcg ccaggcgttc tcataggcgc ggccgtaatc cttcatcatg
     4981 cggttgtagt cggcgatggt ggccaggccg aggccggtca ccaccagcgc catcagcgcg
     5041 aaacaggcca ccagggccat gatgcgggcg cgtatcgaca tgaagatccc tttgcgcggc
     5101 gcaatgcctc agggccggag tctacgctca aacggtaata aatccataaa aaagaccggc
     5161 ggatcgcgct cagctcacgc cgccgcccag cgcggcgtag agaccggcca gattggcggc
     5221 gcgggccagt tgcacggcga tcagggcctg ttgcgcgctg taggcggtgc gctgggcgtc
     5281 gagcaggttg aggtaggaat cgacgccctt gtcgtagcgc gcctgaccga gggtcagggc
     5341 ggcgccggcg gcgttggcgg ccttgcccgt ggcggtcagc cgcgtttcga tgcccgcgcg
     5401 cacggccaga gcgtcggcca cgtcgcggaa ggcggcctgc acggtctttt cgtagctcgc
     5461 gaccgcgatc tgctgcgacg ctctggcgcc cttgaggttg gcgaggttgt agccgccgcg
     5521 gaagatcggc aggctgagcg acggcgcgaa gctccaggcg ccggttcccg acgtgaacaa
     5581 atcgttcagg ctgtcgctgg ccgaaccggt cgagccggtc aggctgacgc tggggaagaa
     5641 ggcggcgcgc gcggcgccga tgtcgccgtc ggcggccttg atcgcgtgtt cggcggccag
     5701 cacgtccggg cggcgggtca gcacgtccga gggcagaccg gcgggcaggt cgctcagcac
     5761 cgcgccgtcc ggcagggtcc gcggcaggtc ggcctcgctc agttgcgttc cggccagcag
     5821 gttgagcgcg tccttgtcct gctgcacctg agtctcgtac tgcgcggcgt cggcgcgcgc
     5881 ctgctcggtc aggatttcca gcgcgcgcag gtcggtttcc gccgccgcgc cgaggtcgaa
     5941 cttgcgctgg gtgaggtcat agccctcctg ccgcgtgcgc agggtttcgc gcgccagccg
     6001 cagcagatcc tgatcggcgg cgagggtgaa ccacgcctgc gcggtttcgc tgatcagcga
     6061 gatctgcgtc gcgcggcgcg tctcgtccga ggcgaggaag ctttccttcg cggcctgcgc
     6121 ctgcgaccgc gccttgccga acaggtccag ctcccatgcg ggaacggcca gggcggcgga
     6181 atagaggtcg ctgtcgacgc ccgcctgatg cgtgcgcgtt tcgccgagat tgccgttcag
     6241 cgccggcaac aggccggagc gcgtcatcag gtacgaggcg cgcgccgtct cgatgttcag
     6301 cgccgccacg cgcaggtcgc ggttgttgag aagcgcggcg tcgatcacat gttgcaggcg
     6361 cgcatcgaga aagacctgct tccacggcag gtcgcccgcg ttccggcctt gagcggcgtc
     6421 ggcgacgggc cagtcctgcg ccaccggcag ggcgggccgc acatatttcg gcgccagcgt
     6481 gcaggcgctc agggccaaag acgtcagggc caaagacgtc agggcgagag aggtcagggc
     6541 aagagttttc atcactatat cctcgatcct cgccttaatg cgccgccgga ggcgtgtcgt
     6601 cgccgcccga tgtgcgttca agctccgcgt cgtcgcgcat ccgttgcagc ttcagcacgt
     6661 gcttttcgtc ggccttgaac agcttttgca ccagcacgaa gaacagcggc acgaagaaga
     6721 tggccaggaa ggtcgccgac aggatgccgc cgatcaggcc ggtgccgatg gcgtgctggg
     6781 cgccggagcc cgcgccgttg gagatggcca gcggcagcac gccgaaggtg aaggccagcg
     6841 aggtcatgat gatcgggcgc aaccggatgc gcacggcctc caaagtcgcc tcggtcaggg
     6901 tgcggccttc ctcgtgcagc gacttggcga attcgacgat caggatcgcg ttcttcgccg
     6961 acaggccgat gatggtcaaa agcccgacct gaaaatagac gtcgttgttc aggccgcgga
     7021 agctcatcgc cagcagcgcg cccagcacgc cgagcgggat gaccatgatg acggccagcg
     7081 ggatcgacca gctttcgtac agcgccgcca ggagcaggaa gacgaccgcg atcgacaggg
     7141 cgtagagggc cgaggcctga ttgccggact gctgttcctg caacgagatg ccggtccatt
     7201 cgtagccgac gccgggcggc agttgcttgg ccagcttctc catctcggcc atggccgcgc
     7261 cggagctttt gccgggcgcc ggggcgccgt cgagctcgac ggcgggcagg ccgttatagc
     7321 gcgtcaggct gggggcgccg aagctccact tttcggtggc gattgaggac agcggcacca
     7381 tctggttgcc gctgttgcgc acgaaccagc ggtcgagatc ctcgctcttc atgcggaacg
     7441 gcgcgtcggc ctgcacatag accttcttga cgcggccgcg gtcgatgaag tcgttgacat
     7501 aggcgccgcc ccacgcggtc gacaacacgc tgttgacgtc ggacagggtc aggttcagcg
     7561 ccccggcctt ggcctgatcg atatcgacgt cgagctgggc ggtgtcctcc tgaccgttcg
     7621 ggcgcacggc gctcaaaagc gggtctttcg ccgccatgcc cagcaactgg ttgcgcgcct
     7681 gaatcagctt gtcgtggccg acgccgccct gatcctcaag catcatcgta aagcccgacg
     7741 aggcgccgag cgacgagatg gcgggcggca cgatcgcata gatggtcgcg ttcggataac
     7801 cggcgaagtg catgatgccg cgcatggcca cggcctgcgc cttgtccttc gaggcgtggc
     7861 gcttgtcgaa gtccttgagg cgcacgaagg ccatcgccat gttctgcccc tggccgccga
     7921 agctgaagcc ggtgacgccg aacacggcgc tgaccgccgg gtctttctgg aagtatcgcc
     7981 gcacgtcgtc gacgaccttc tgcgtgcgct cctgcgtggc cccggcgggc aactggatca
     8041 gggtgaagac gatgccctga tcctcctccg gcaggaaggc ggtcggcagg cgcgtgaaca
     8101 gaagcgcgac cgccccgatg atcagcgcga agccgatcat ccagcgcgcg ggctgggtca
     8161 ggatcttgcg caccgtcccc tgatagcggt tgctggcggc gtcgaagcgg tggttgaacc
     8221 agccggcgag gccggtctgg atatggccct cgcccttttt caccggcttg agcagggtcg
     8281 cgcacagggc gggcgtcagg atcagcgcca ccgtgaccga cagcgccatg gccgacacca
     8341 gggtcaccga gaactggcgg tagatcatgc cctgcgagcc ggtgaagaag gccatcggca
     8401 cgaacacggc ggccagcacc agcgcgatgc cgatcagggc gccggtgatc tcgcccatcg
     8461 acttgcgcgt cgccacgcgc ggcggcagcc cttcctcgga catgatgcgc tcgacgttct
     8521 cgatcaccac gatggcgtcg tcgaccagca gcccgatggc cagcacgagg ccgaacaggg
     8581 tcagggtgtt gatcgaataa ccgaccgcgg ccagaatccc gaacgtgccc agaagcacga
     8641 ccggcacggc gatggtcggg atcagggtcg cgcgccagtt ttgcaggaag acgtacatga
     8701 cgaggaagac cagcaccacg gcctcgacca gcgtcttgac gacctcctcg atcgacaggc
     8761 tgatgaacgg cgtggtgtcg tagggcacga catagtgcat gcccgccggg aagctcctgg
     8821 acagctcgtc catccgcgcc ttgaccgcct tggcggtgtt gagcgcgttg ccgcccggcg
     8881 tcagcttgac cgcgatgccg gacgccggat ggccgttata ggtcgccgac gaggcgtagc
     8941 tttccgcgcc cagctcgacg cgcgccacgt cgctcaggta aaccttggcg ccgtcgctct
     9001 gcgtcttgac gatgatgttg cggaactgct ccggcgtggt caggcgcgac tgcgccgtga
     9061 tggtggcgtt gagcatggtt cccggcacgg cgggcaggcc gcccagttcg cccgcggaca
     9121 cctgcgtgtt ctgcgcctga atcgccgccg tcacgtcgga cggcatcagg ttgtagctgg
     9181 ccagtttctg cgggtcgagc cagatgcgca tcgcgtactg ggcgccgaac aactgcacgt
     9241 cgccgacgcc gtcgacgcgc gacaactggt ccttgatcga cgagccgata tagtcggaaa
     9301 tgtcggtctg ggtcgtcttg ccgtcgtcgg cgtacacccc gacgaccagc aggaagttgg
     9361 aggtcgattt cacgaccgac acgccctgcg cctgcaccga ttgcggcagc agcgccatga
     9421 cggtctgcag cttgttctgc acctgcacct gcgcgatgtc gg
//
LOCUS       DASVLL010000186         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_495140,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000186 DASVLL010000000
VERSION     DASVLL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495140"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VF402_03100"
     CDS             complement(<1..834)
                     /locus_tag="VF402_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7799309.1"
                     /translation="MSSFQAELTRLKTAGRYRALRPRAGIDLSSNDYLGLSDHPDLRR
                     AAIAALQGGLVLGSGGSRLLSGHHDAHADLEAFAARHFGVPRALYFATGFQANYALTT
                     ALIGRHDVAIFDALSHASLRDGLHAVHGRAIKVRHNDLDGFEAALRRPRPARAFVLVE
                     SAYSMDGDLAPVADLLRLCERYDATLIVDEAHGTGVLGATGKGLTEGLASERLITLHT
                     CGKALGVAGALVCAGDDVIDYLINRARPFIYSTAPMPLQALLTQKALQIAAREGWRRE
                     RL"
     gene            complement(928..1569)
                     /locus_tag="VF402_03105"
     CDS             complement(928..1569)
                     /locus_tag="VF402_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7799310.1"
                     /translation="MTTAKNDPIAMRISRVLADRIISGAFAPGEKLPQDRIAEEFGAS
                     HVPVREAFRRLEAQGLVVSEPRRGVRVAGFSADEVREVAEMRAALETLALRRAAPHLT
                     PAILDHAEEATLAGDRAGDVQAWEAANRRFHRLILTPCAMPRLLKAIDDLHAVSARFL
                     FSGWRAEWEAPTDRDHRAILTALRAGDVEAAALILSHHVLGAGQKPAKRRTKG"
     gene            1743..>2347
                     /locus_tag="VF402_03110"
     CDS             1743..>2347
                     /locus_tag="VF402_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799311.1"
                     /translation="MSYRFSSARRLRGVLIVTTALSALAGGAFAASVDHYTVTKNGVT
                     LEISAPRADIIRVRAGDPNLPEDASWAVSQDVRARHAPLDVTEAAGVATVSTGAASVK
                     IDETTLAVTVYDKAGHVVLADAKGAALQFDPDRLGQGFRLRKAMPVDAHYFGLGDKTG
                     PLDRRNEAFTLWNTDNFGFGVATDPLYKSIPFVLGVDEKGQ"
BASE COUNT          363 a          778 c          836 g          370 t
ORIGIN      
        1 caggcgctcg cggcgccagc cctcgcgcgc ggcgatctgc aacgcctttt gcgtcagcag
       61 cgcctgcaac ggcatcggcg cggtcgaata gatgaacggg cgcgcgcggt tgatcaggta
      121 gtcgatcacg tcgtcgcccg cgcagaccag cgccccggcg acgcccagcg ccttgccgca
      181 ggtgtgcagc gtgatcaggc gctccgacgc cagcccttcg gtcagcccct tgccggtggc
      241 gcccagcacg cccgtgccgt gcgcctcgtc gacgatcagg gtcgcgtcat agcgttcgca
      301 caaacgcaac aggtccgcca cgggcgcgag gtcgccgtcc atgctgtatg ccgactcgac
      361 cagaacgaag gcgcgcgccg ggcgcgggcg gcgcagcgcg gcctcgaagc cgtcgagatc
      421 gttgtgccgg accttgatcg cgcggccatg aacggcgtgc aggccgtcgc gcaggctggc
      481 gtggctgagg gcgtcgaaga tcgccacgtc atgccgcccg atcagggcgg tggtcagcgc
      541 gtaattggcc tgaaaaccgg tggcgaaata gagcgcgcgg gggacgccga aatgccgcgc
      601 ggcgaaggct tcgagatcgg cgtgggcgtc gtgatggccg ctgagcaggc gcgagccgcc
      661 cgaaccgagg acgaggccgc cttgcagcgc cgcgatggcg gcgcggcgca ggtcggggtg
      721 atcgctcagg cccagatagt cgttggacga caggtcgatt ccggcgcgcg gacgcagggc
      781 gcgatagcgt ccggccgttt tgagacgcgt cagttcggcc tgaaaggagg acatgcggca
      841 aaattataga taaaattacg gtttctgcgc caaggagtcc ataatttacg cgccgacgca
      901 agccgtttgg cgataaaaat agataattta gccttttgtg cgccgtttcg ccggtttttg
      961 cccggcgccc agcacatgat gcgacaggat cagcgccgcc gcctcgacgt cgcccgcgcg
     1021 cagggcggtc aggatggcgc ggtggtcgcg gtcggtcggc gcctcccatt cggcgcgcca
     1081 gccggagaac aaaaagcggg cgctgaccgc gtgcaggtcg tcgatggcct tcaacaggcg
     1141 cggcatggcg cacggcgtca ggatcaggcg gtgaaagcgc cggttggccg cttcccacgc
     1201 ctgcacgtcg cccgcgcggt cgcccgccaa agtcgcctcc tcggcatggt cgagaatcgc
     1261 cggggtcagg tgcggcgcgg cgcggcgcag ggccagcgtc tccagagcgg cgcgcatctc
     1321 ggcgacctcg cgcacctcgt ccgcggaaaa accggcgacg cgcacgccgc ggcgcggctc
     1381 gctgaccacc aggccctgcg cctccagccg ccggaaggct tcacgcaccg gcacgtggct
     1441 ggcgccgaac tcctcggcga tgcggtcctg cggcagcttc tcgccgggcg cgaaggcgcc
     1501 ggaaatgatg cggtcggcca atacgcggct gatgcgcatg gcgatggggt cgttcttggc
     1561 ggtcgtcatg gttttataga taatttgcgc tggccgcttc gtccagccgc ttccgcgcgg
     1621 cgggcggcgc gattgaattc gtattcactg ccgccgcccg cgtttgcgcc ttgattttcg
     1681 cggcgcgcgc cgttcttgtg cgcacaacaa ggccgcgccg tcccagcggt cgggaggatg
     1741 tcatgtctta tcgcttttcg tccgcgcgcc gtctgcgcgg cgtcctgatc gtcacgaccg
     1801 ccttgtcggc gctggcgggc ggggccttcg ccgccagcgt ggatcattac accgtgacga
     1861 agaacggcgt gacgctggag atcagcgcgc cgcgcgccga catcatccgg gtgcgcgcgg
     1921 gcgatccgaa cctgccggag gacgcctcat gggcggtttc gcaggacgtg cgcgcgcggc
     1981 atgcgccgct cgatgtgacc gaggcggcgg gcgtggccac cgtctcgacc ggcgcggcct
     2041 cggtgaagat cgacgagacg acgctggccg tcaccgtcta tgacaaggcg ggccatgtcg
     2101 tgctggccga cgccaaaggg gcggcgctgc aattcgatcc cgaccgtctg gggcagggct
     2161 tccggctgcg caaggcgatg ccggtcgacg cgcactattt cggcctcggc gacaagaccg
     2221 ggccgctgga ccgccgcaac gaagccttca ccctgtggaa caccgacaat ttcggcttcg
     2281 gcgtcgcgac cgacccgctc tataagtcga tcccgttcgt gctgggcgtc gatgagaagg
     2341 ggcagag
//
LOCUS       DASVLL010000187         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_225427,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000187 DASVLL010000000
VERSION     DASVLL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225427"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..614)
                     /gene="trxA"
                     /locus_tag="VF402_03115"
     CDS             complement(288..614)
                     /gene="trxA"
                     /locus_tag="VF402_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7799312.1"
                     /translation="MATHKITDESFESDVLKSDTPVLVDFWAEWCGPCKQIAPILEEL
                     AEGLGEKVKIAKLNIEDSPMTPSRFGVRGIPTMILFKGGQMASMKVGAMPKAKLLEWL
                     GENEIA"
     gene            complement(671..>1661)
                     /locus_tag="VF402_03120"
     CDS             complement(671..>1661)
                     /locus_tag="VF402_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX7799313.1"
                     /translation="ARLRALREDKAARDEQEGLRLLYVALTRARDRLTLCGFAGKRAP
                     KPGRFPGWYDLLDQAFDQLESGEEVMALRAPFDPQEMTREAVIRTFGARSPVQTPEAG
                     AVAPPVDLPAFALAEVPQAQVDLERWRAVSQMGDDERPDDERAPSPLAQANGLGRYRR
                     GELIHKLFEILPEIAPDRRAGIAARYLERQPDLNPSQRREMAQAVMTVLTHERFAEAF
                     GPGSRPEVALAGQIGVNASGEAVMLSGRIDRLVIAPGRVLIIDYKSNRPAPDRAEDAA
                     VAYQRQMAGYVALLRRIHPDKAVEAALLWTDGPKLTPLSEALVNLRLAEILAR"
BASE COUNT          269 a          582 c          528 g          282 t
ORIGIN      
        1 gccctgccgc cgggaccgcg ccgcgtgctg atctgcggct cgctctatct ggcgggcgac
       61 gtgctggcgc tgtcgccgca gacgtggccg aggtaagaat tttcgccacg aaaggcacga
      121 aaaggcacga aaatctggcg aagccgacca ctcaatttat ttcaatgacg ccggcagaca
      181 tgaacgcagc gcacacactc tctttcgtgc cttttcgtgc ttttcgtggc caataaaaaa
      241 ccccgccgca aagcgacggg gcttcatctc cacggtcttg cccgaaatca cgcgatttcg
      301 ttttcgccca gccattccag cagcttggcc ttgggcatgg cgccgacctt catcgaggcc
      361 atctggccgc ccttgaacag gatcatggtc gggatgccgc gcacgccgaa gcgcgacggg
      421 gtcatcgggg aatcctcgat gttcagcttg gcgatcttga ccttttcgcc cagcccttcg
      481 gccagttctt cgagaatcgg cgcgatctgc ttgcacgggc cgcaccattc ggcccagaaa
      541 tcgaccagaa ccggcgtgtc ggacttcagc acgtcgcttt caaagctttc gtcggtgatc
      601 ttatgcgtcg ccatgtctgg ctccctaaaa acgagttcgg ccctgaatgt aggcatggcg
      661 gcggcggaaa tcaacgcgcc agaatttccg cgagcctgag atttactaac gcctcgctta
      721 agggcgtcag cttcggcccg tcggtccaca acagcgcggc ctcgaccgcc ttgtccgggt
      781 ggatgcgccg caacagcgcg acatagcccg ccatctggcg ctgataggcg acggcggcgt
      841 cctcggcgcg gtcgggcgcg gggcggttgg acttataatc gatgatcagc acccggccgg
      901 gcgcgatcac cagccggtcg atgcgcccgg acagcatgac cgcctcgccc gacgcgttga
      961 cgccgatctg tccggccagc gcgacctccg gccgcgatcc cggcccgaag gcttcggcga
     1021 agcgttcatg cgtaagcacc gtcatgacgg cctgcgccat ctcccgccgc tgcgacggat
     1081 tgaggtccgg ctggcgctcc agatagcgcg ccgcgatccc ggcgcgccgg tccggggcga
     1141 tctcgggcag gatctcgaac agcttgtgga tcagttcgcc gcgccggtag cggcccagcc
     1201 cgttcgcctg cgccagcggc gacggcgcgc gttcgtcgtc ggggcgctcg tcgtcgccca
     1261 tctgcgacac cgcgcgccag cgttcgagat cgacctgcgc ctgcggcacc tcggcgaggg
     1321 cgaaggcggg caagtcgaca ggcggcgcga cggccccggc ctccggcgtc tgaacggggg
     1381 agcgcgcgcc gaaggtgcgg atcacggcct cgcgcgtcat ctcctgcggg tcgaaaggcg
     1441 cgcgcagggc catgacctcc tcgccgctct caagctgatc gaaagcctgg tccagcaagt
     1501 cataccagcc ggggaaacgg cccggcttcg gcgctctttt ccccgcgaag ccgcacaggg
     1561 tcagccggtc gcgcgcccgc gtcagcgcca cgtacagaag ccgcagcccc tcctgctcgt
     1621 cgcgcgcggc cttgtcctcg cgcagggccc gcagccgcgc c
//
LOCUS       DASVLL010000188         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_225491,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000188 DASVLL010000000
VERSION     DASVLL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225491"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF402_03125"
     CDS             <1..215
                     /locus_tag="VF402_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX7799314.1"
                     /translation="AYHRTRPLSEAEIAALPLLARGAAVRFTLTRLYDLLNHDPSWVV
                     KPKDPEAFYRRLDYHRRIADGRAYFG"
     gene            661..1753
                     /locus_tag="VF402_03130"
                     /pseudo
     CDS             661..1753
                     /locus_tag="VF402_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
BASE COUNT          330 a          558 c          626 g          318 t
ORIGIN      
        1 acgcctatca caggacgcgc cccctgtcgg aggccgagat cgccgccctg cccctgctcg
       61 cgcgcggcgc ggcggtgcgg tttacgctga cgcggctcta tgaccttttg aaccacgatc
      121 caagctgggt ggtgaagccg aaagacccgg aagcctttta ccggcggctg gactatcacc
      181 ggaggatcgc ggacgggcgg gcctatttcg ggtagtgcgg caaagccaca caggtatcat
      241 ttgaaatcgg accgcttgac cggcgcgcgc gccgggcgca aaacgcggtt atgtttacga
      301 aacgcagcca tgcccagacc ctttgcgcgc ggacagttcg ctctgtcggc gccaataggg
      361 ctgcgtccgt tccgccgcaa gcggcggaga cggaggttct gtcaatgggc tgtcggccga
      421 ggctttagac tcgcgccgac atcgtggttt tcgcacagcc cttttgacct ccgtcaaaag
      481 ggctttttcg tacgcatatc ttcatcaaca ccaagaagga tcaaagacat gcaagtcttc
      541 aaaggcgcca ggggcgggct gatcaaggcc tggatcgacg gcgtggccgt ggaagatact
      601 gcacgcgccc agttggacaa catcgccgcc atgccgttca ttcacgggca cattgcggtg
      661 atgccggacg ttcactgggg tatgggcgca accgtgggat cggtcatccc gacgcgcggc
      721 gcgatcattc cggcggcggt cggcgtcgat ctcggctgcg gcatgatggc tgtgcgcacg
      781 accttgcggg ccgccgatct gcccgacgat ctgtcgggac tgcggacggc gatcgagcaa
      841 cggattccgc acgggcggac ggacaacggc ggccgcaacg accgcggcgc ctggggcgac
      901 gccccgcagg ccgtggacgc ggttttcggg gaactgagcg acggactgaa caagatcgtc
      961 gagaagcatc ccaagatcgc gcaggcggcg gtccgggcct ccagccatct cggcacgcta
     1021 gggaccggca accatttcgt cgaggtgtgc ctcgatgaaa acgacgccgt gtggatcatg
     1081 ctgcattccg gttcgcgcgg aatcggcaac aggatcggcg cctacttcat cgagcgcgcc
     1141 aaggccgaaa tgcggcggtg gttcatcaac ctgccggatg aggatctggc ctatctgccg
     1201 caaggctcgg aactgttcgg cgactatatc ggcgccgtgt cgtgggcgca gcgttttgcg
     1261 cgcgccaacc gggagctgat gatgacggcg gcgcttgagg cgttgtcggc ggcggttccc
     1321 aagcgtttca cctgcgactg cgaggctgtg aactgtcacc acaattatgt ggcgcaggaa
     1381 cggcatttcg gcgcggacgt ctatgtgacg caagggcgcg gtgcgcatca cgtcgcagac
     1441 gctgggcatc attcccggtt cgatgggggc gaagtccttc atcgtgcggg gcgtggacgg
     1501 cgcggcggcg gcggactccc tgtgttcgtg ttcgcacggc gccgggcggg ccatgtcccg
     1561 cgccgaggcc agacggcgct tttcggtcgc cgaccacatc gcggcgaccg aaggcgtcga
     1621 gtgccgcaag gacgccgacg tgatcgacga aacgccggcg gcctataagg acatagacac
     1681 ggtcatgaag gcgcaggccg atcacgtcga aatcgtgcat accctcaagc agatcgtctg
     1741 cgtgaagggc taaaactaaa gccggcggga ggctcccgcc ggctttttca gatatccggc
     1801 gtcagttggc cgccgccggg gaggggccag gt
//
LOCUS       DASVLL010000189         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_495274,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000189 DASVLL010000000
VERSION     DASVLL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495274"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..921)
                     /locus_tag="VF402_03135"
     CDS             complement(46..921)
                     /locus_tag="VF402_03135"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX7799315.1"
                     /translation="MTIQTVGVIGAGQMGAGIAQVCAQGGYAVKLFDQSPDAVRAALG
                     RVEKGLNRLVEKEAIADADAKAALGRLSAADDVAGLADCDLVIEAATENEAVKNAIFK
                     SVCAHLKPEALLASNTSSISITRLAAATDRPGKFIGLHFMNPVPVMKLVEIIRGIATE
                     AGTYEAAVAFAQSLGKTTANSEDFPAFIVNRVLIPMINEAVYTLYEGVGTVEAIDTAM
                     KLGANHPMGPLELADFIGLDTVLAIMNVLYDGLADSKYRPCPLLVKYVEAGWYGRKTG
                     RGFYDYRGEKPVPTR"
     gene            1122..>2175
                     /locus_tag="VF402_03140"
     CDS             1122..>2175
                     /locus_tag="VF402_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX7799316.1"
                     /translation="MPDTTAAPQHLVLIDGSGYIFRAYHGLPPLTRKSDGLPVGAVAG
                     FCNMLYKLIKEHRGDDAPSHLAVIFDAGAKTFRNEIYDQYKAHRPPAPEDLIPQFPLI
                     REATRAFGLPAIELEGFEADDLICTYTKLAVEAGMAVTIVSSDKDLMQLVDDARHVRL
                     MDPVKSTPVHAAQVIEKFGVPPELVVDAQALIGDSSDNVPGAPGIGVKTAAQLLSDFG
                     DLDGVLSRAAEIRQEKRRQTLIDFKDQILMSRELVRLRCDVAPPAPIDDFVLRDADGE
                     TLTAFLTTMEFATLARRVGGTLSALQSGTAMDKIPTAPILRPLYGAKPAAPAEAATAP
                     FDRDAYACVQTLDDLNA"
BASE COUNT          362 a          749 c          692 g          372 t
ORIGIN      
        1 cggcgaaggc cagccacaaa ccgtcaggtt gccgcaggcg caggctcacc gcgtcggaac
       61 cggcttttcg ccgcgatagt cgtagaagcc gcgcccggtc ttgcgcccgt accagcccgc
      121 ctcgacatat ttgaccagca gcggacacgg gcggtacttg gaatcggcca gcccgtcata
      181 cagcacgttc atgatcgcca gcaccgtgtc cagcccgatg aagtcggcca gttccagcgg
      241 ccccatcggg tgattggcgc ccagcttcat cgccgtgtcg atggcctcga ccgtgccgac
      301 gccttcatac agcgtataga ccgcctcgtt gatcatcggg atcagcacgc ggttgacgat
      361 gaaggccgga aaatcctcgg aattggccgt ggtcttgccc aagctctgcg cgaaggcgac
      421 ggcggcttca taagtgccgg cctcggtggc gatgccgcgg atgatctcga ccagcttcat
      481 caccggcacc gggttcatga agtgcaggcc gatgaacttg cccggccggt cggtggcggc
      541 ggccagccgc gtgatcgaga tcgacgaggt gttcgaggcc agcagcgcct cgggcttgag
      601 atgcgcgcac accgacttga agatggcgtt cttgaccgcc tcgttctcgg tcgccgcctc
      661 gatgacgagg tcgcagtcgg ccagccccgc tacgtcgtcg gcggcgctca ggcggcccag
      721 cgcggctttg gcgtcggcgt cggcgatcgc ctctttctcg accagccggt tcagtccctt
      781 ctcgacgcgg cccagcgcgg ccctgacggc gtcgggcgac tggtcgaaca gtttgaccgc
      841 atagccgccc tgcgcgcaca cctgcgcgat gccggcgccc atctggcccg cgccgatcac
      901 gcctacggtt tggattgtca tactttttag tccttgcgga aattcatcca ttggccgcga
      961 ttagggcgca aatttcacgc gaaggcaagg cgcggcgcgc ggcctgagcg aggccgcgga
     1021 caaagctgtg gatatttcca ctgacattta cggtcgcttt gatttggcag cctccccata
     1081 tgccgctatg aacagcgcct atgccgacac aagagccccc catgcccgat acgaccgccg
     1141 cgccccaaca tctcgtcctg atcgacgggt cgggctatat tttccgcgcc tatcacggcc
     1201 tgccgcccct gacgcgcaag tcggacgggc tgccggtcgg cgcggtggcg ggcttttgca
     1261 acatgctcta caagctgatc aaggagcatc gcggcgacga cgcgccgtcg catctggccg
     1321 tcatcttcga cgccggggcc aagaccttcc gcaacgagat ctacgaccag tacaaggcgc
     1381 accgcccgcc cgcgccggaa gacctgatcc cgcaattccc gctgatccgc gaggcgacgc
     1441 gcgccttcgg cctgcccgcg atcgagcttg agggtttcga ggccgacgac ctgatctgca
     1501 cctacacgaa gctggcggtc gaggccggga tggcggtgac catcgtctcg tccgacaagg
     1561 atctgatgca actggtcgac gacgcgcgcc acgtccggct gatggacccg gtcaaatcga
     1621 cgccggtgca tgccgcgcag gtgatcgaga agttcggcgt gccgccggag ctggtggtcg
     1681 acgcgcaggc cctgatcggc gattcgtccg acaatgtgcc cggcgccccc ggcatcggcg
     1741 tcaagaccgc cgcgcagttg ctgagcgatt tcggcgatct cgacggcgtt ctgtcgcgcg
     1801 ccgcggagat caggcaggag aagcgccgcc agaccctgat cgactttaag gaccagattc
     1861 tgatgtcgcg cgagctggtg cgcttacgct gcgacgtcgc gccgcccgcg ccgatcgacg
     1921 acttcgtgct gagggacgcc gacggcgaga cgctgaccgc ctttttgacc acgatggagt
     1981 tcgccacgct ggcgcgccgc gtcggcggga cgctgagcgc cctgcaatcg ggcacggcga
     2041 tggacaagat tcccaccgcg ccgatcctgc gcccgctcta cggcgccaaa ccggccgcgc
     2101 ccgccgaggc cgcgaccgcg ccgttcgatc gcgacgccta tgcgtgcgta cagacgctgg
     2161 acgacctcaa cgcct
//
LOCUS       DASVLL010000190         4468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_441339,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000190 DASVLL010000000
VERSION     DASVLL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441339"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VF402_03145"
     CDS             complement(<1..994)
                     /locus_tag="VF402_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799317.1"
                     /translation="MSAGLEKAAPGAGMALWASTIAFTACFAAWTIFAIIGVQIKTDL
                     GLSDAEFGLLAGAPILTGSLARIFLGVWADQFGGRTVNLLVMLTSSAATFLLSYARTY
                     PQFLLAALGVGLAGGAFAVGVAYVSRFYPKSRQGAVLGIFGAGNIGAAFTKIAAPIVM
                     AAAGWTGVARAWAVGLAVIGVAAFLLTRDDPDLTARRRVKARPVSTFAQLAPLRNMQV
                     WRFALYYFFVFGGFVSLALWLPHYLVGVYRLDLKTAGLFAAAYSIPASLFRAAGGALA
                     DKIGARKVMYLTFGVSLICCFLLAYPATSYVVDGVRGPIRFRLATGLIPFTIIIF"
     gene            1287..2051
                     /locus_tag="VF402_03150"
     CDS             1287..2051
                     /locus_tag="VF402_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7799318.1"
                     /translation="MRTADLDRIRALTLFSGMSDESFHAVTAGAYMQNFPAGTTLLYE
                     GDPVDFLYIVMDGAVELQGTWNDKESTLAILRPISTFILAAVVLDANGLMSARTLERS
                     QILMLPAETFRKVMQENAHFAMTVAREVSGCYRGLVRSIKNQKLRNGAERLANYLLTQ
                     QVRQGGGPSITLPHEKRTLASLLGMTPENLSRAFATLTDYGVEINGPEVTITRPVPLK
                     RLAKPSPLIDNHAPASATFAGKAEKELWPDDAQLLH"
     gene            complement(2052..3044)
                     /gene="moaA"
                     /locus_tag="VF402_03155"
     CDS             complement(2052..3044)
                     /gene="moaA"
                     /locus_tag="VF402_03155"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX7799319.1"
                     /translation="MGFDNRSLMDAQGRRFPYVRLSITEVCNFRCAYCLPDGWKKTGP
                     LHFLRPEEIGRLASALTEVGVGKVRLTGGEPSVRKDLPEILATLAAVSGMAKIAMTTN
                     GWNLERRSASWRESGLTHLNVSVDALDADAFHRITGHDRLTSVLRGIDDALARDYASV
                     KLNAVLLRETAEKGFADWARFVKDRPVAVRFIELMRTADNADYFAAHHVSGAGLRDWL
                     TERGWTPLQRRSDDGPAVEFVHADHRGRIGLIAPYAPGFCDSCNRLRVTARGKLRLCL
                     FGEGGVDLRDLLQVPDDRSELVRRIHAALYGKAAGHRLHQGLPGDLRNLAELGG"
     gene            complement(3137..4303)
                     /locus_tag="VF402_03160"
     CDS             complement(3137..4303)
                     /locus_tag="VF402_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX7799320.1"
                     /translation="MRSYRGPAVLSAGFRPFFLAASIWAAVMAPLWVWVYTGKGRIPP
                     GASLMWHGHEMLFGYLGGVIAGFLLTAIPNWTGRLPVTGTPLLLLFLSWLAGRAVMMT
                     PWWTGWPGLLGDGLFLTVFAGVVGREVWIGKNWRNLPVAVMVGALALCNIAFHLGVRE
                     ASIRLAAGVITTLICLIGGRIIPSFTTNWLKKRGAETLPTPFNRFDLIVVSGTAAAML
                     VWALAPVAVPTGLLLCAVGVGHAIRLCRWRGGKVWGEALLWILHVGYAWVAAGLILVG
                     LASLTGAAGLGFALPAAGCLHALTAGAMTVMSLAVMTRAILGHTGRELHAGPGVTALY
                     VLANLAAVVRVAAALFPAAYVFGLTLSGYLWAGAFGLFLCLFGPKLVGPRLAKA"
BASE COUNT          859 a         1538 c         1425 g          646 t
ORIGIN      
        1 cgaagatgat gatcgtgaag gggatgaggc ccgtggcgag ccggaaacgg atcggccccc
       61 ggacgccgtc gacgacatag ctggtcgcgg gataggccag caggaagcag cagatcaggc
      121 tgacgccgaa cgtcaggtac atgaccttgc gcgcgccgat tttgtcggcc agggcgccgc
      181 cggcggcgcg gaaaagggac gcggggatcg aataggcggc ggcgaacaga cccgccgttt
      241 tcagatcgag acgatagacg cccaccagat agtgcggcag ccacagcgcc agcgacacga
      301 agccgccgaa cacgaagaaa tagtagagcg caaaacgcca gacctgcatg ttcctgagcg
      361 gcgccagttg cgcgaaggtg gagaccggcc gggccttgac ccggcgccgc gccgtcaggt
      421 cgggatcgtc gcgcgtcagc aggaaggcgg cgacgccgat gacggccagc ccgaccgccc
      481 aggcccgcgc cacgccggtc cagcccgccg ccgccatgac gatgggcgcc gcgatcttgg
      541 tgaaggccgc gcctatattg ccggcgccga atatgcccag cacggcgccc tgacgggact
      601 tgggatagaa gcgcgagaca taggcgacgc cgaccgcgaa ggcgcccccg gccagaccga
      661 cgcccagcgc ggccagcagg aattgcggat aggtgcgcgc gtaggacagc aggaaggtcg
      721 ccgccgacga cgtgagcatg accagcaggt tgaccgtccg gccgccgaac tgatcggccc
      781 atacgccgag gaaaatccgc gccagcgaac ccgtcagaat cggcgccccg gccagcaggc
      841 cgaactcggc gtcgctcagg cccaggtccg tcttgatctg cacgccgatg atggcgaaga
      901 tcgtccaggc ggcgaagcag gccgtgaacg cgatcgtgct cgcccataag gccatgcccg
      961 cgcccggagc cgctttctca agtcctgccg acatgcctct gtccttccgg gcgatagctc
     1021 gcgtcggggt tagagatatg tgaagggcgg cgcggatcgc ttgacagtta tcaatccgtc
     1081 ggacgactag cgtcagatgg ccgcatatac tgtgtaagac ggttggaaag actggtcttt
     1141 ttctttgata cggatgcccg tcgctgcaag ggcggccgtc ttcgcccgca ggcctacgtg
     1201 tttatacgta ggggctttca tcaattgaca attatcaacc ggaaacgacc ctgcgcatgt
     1261 catgagtgga ggagtaggag ttcccaatgc gcaccgctga tcttgaccgc atccgcgccc
     1321 tgaccctgtt ttccggcatg tcggacgaaa gcttccatgc ggtgaccgcc ggcgcctata
     1381 tgcagaactt cccggcgggc acgaccctgc tctacgaagg cgatccggtc gatttcctct
     1441 atatcgtcat ggacggcgcc gtcgaattgc aggggacgtg gaacgacaag gaaagcacgc
     1501 tggccatcct gcgtccgatc tccaccttca tcctggccgc cgtcgtgctg gacgccaacg
     1561 ggctgatgtc ggcgcgcacg ctggaacggt cgcagatcct gatgttgccc gccgaaacct
     1621 tccgcaaggt gatgcaggag aacgcccatt tcgccatgac ggtggcccgc gaggtgtcgg
     1681 gctgttatcg cggcctcgtg cgttcgatca agaaccagaa gctgcgcaac ggcgccgagc
     1741 ggctggccaa ctatctgctg acccagcagg tgcggcaggg cggcggcccg tccatcaccc
     1801 tgccccatga aaagcgcacc ttagcctctc ttctgggcat gacgccggaa aatctgtccc
     1861 gcgcctttgc gaccctgacg gactacggcg tcgagataaa cgggccggag gtcacgatta
     1921 cccgcccggt cccgctaaaa cgcctcgcca agcccagccc gctcatcgac aatcacgcgc
     1981 ccgcctcggc caccttcgcc ggcaaggcgg aaaaggaact gtggcccgac gacgcgcaac
     2041 ttctgcactg atcagccgcc aagctcggcg aggttgcgca ggtcgcccgg caggccctga
     2101 tgcaggcgat gcccggcggc cttgccgtac agggccgcat ggatacggcg cacaagctcg
     2161 gatcgatcgt ccgggacttg cagcaggtcg cgcagatcca cgccgccttc gccaaagagg
     2221 cacaggcgca gttttcccct cgcggtgacg cgcagccggt tgcagctatc gcaaaaaccg
     2281 ggcgcataag gggcgatcag gccgatccgg ccccggtggt ccgcatggac gaattcgacg
     2341 gccgggccgt cgtccgatcg cctctgcaaa ggcgtccagc cgcgttccgt cagccagtcg
     2401 cgcagcccgg cgccggaaac atggtgggcc gcgaaatagt cggcgttgtc cgccgtccgc
     2461 atcagttcga taaagcggac cgcgacgggc cggtccttta cgaatcgcgc ccagtcggcg
     2521 aagccttttt ccgccgtctc tctcagcagg accgcgttca gcttgaccga cgcatagtcc
     2581 cgcgccagcg cgtcgtcgat cccgcgcagc acagaggtca ggcggtcatg gccggtgatg
     2641 cggtgaaacg catcggcgtc gagcgcatcg acgctgacgt tcaggtgggt caatccgctt
     2701 tcgcgccagc tcgccgagcg gcgctccaga ttccatccgt tggtcgtcat ggcgattttc
     2761 gccatgccgg aaacggccgc gagggtggcg agaatctccg gcagatcctt cctgacggac
     2821 ggctcgccgc cggtcaggcg gaccttgccg acgccgacct cggtcagggc cgaggccaga
     2881 cggccgattt cctccggccg gagaaagtgc agcgggccgg tctttttcca tccgtccggc
     2941 agacagtagg cgcagcggaa attgcagacc tcggtgatgg acagcctgac atacgggaaa
     3001 cggcggccct gcgcgtccat gaggctgcgg ttgtcgaaac ccatcttgct tcccgtactc
     3061 ctgatcatca ggctcaggct tactacaagt ccgcccgctc cgcctttgac aggcgccgcc
     3121 cgccggttga tgattatcaa gccttggcca gccgggggcc gacaagcttc ggtccgaaaa
     3181 ggcagaggaa caggccaaag gcgcccgccc ataaataacc cgaaagcgtc agcccgaaga
     3241 cgtaggcggc gggaaacagc gcggcggcca cgcgaaccac ggcggcgagg ttggccagaa
     3301 cgtacagcgc ggtcacgccc ggaccggcat gaagctcgcg gcccgtatgg cccaggatcg
     3361 cccgcgtcat gaccgccagc gacatgacgg tcatggcccc cgccgtcagg gcgtgcagac
     3421 agcccgccgc cggaagcgca aagcccagtc cggcagcgcc cgtcaggctg gccagtccca
     3481 cgaggatcag gcccgccgcc acccaggcgt agccgacatg cagtatccac agcagagcct
     3541 cgccccagac cttgccgccg cgccagcggc aaagccggat cgcatggccg acgcccacgg
     3601 cgcacaggag aaggccggtc gggaccgcga ccggcgccag cgcccagacc agcatcgccg
     3661 ccgccgtgcc ggacaccacg atgaggtcga acctgttgaa cggcgtcggc aaggtttccg
     3721 cgccgcgctt tttcagccag ttggtggtga agctcggaat gatccggccg ccgatgaggc
     3781 agatcaaggt ggttataacc cctgccgcca gccggatcga cgcttcccgg acgccgagat
     3841 ggaaggcgat attgcatagg gccagggcgc cgaccatgac ggccacgggc aggttgcgcc
     3901 agttcttccc gatccacacc tcgcgcccca cgacgccggc gaacacggtc aggaaaaggc
     3961 cgtcgcccag cagtccgggc cagcccgtcc accacggcgt catcatgacc gcccgcccgg
     4021 ccagccagct cagaaacagc agcagcaagg gcgtccccgt caccggcagg cgcccggtcc
     4081 agttgggtat cgccgtcagc agaaaaccgg cgatcacccc gcccagatag ccgaacagca
     4141 tctcatggcc gtgccacatc aggctcgccc ccggcggtat ccggcccttg cccgtgtaaa
     4201 cccagaccca cagcggcgcc atgaccgccg cccagatcga ggcggccaga aagaacggac
     4261 gaaagcccgc gctcaggacc gccggtcccc gataggaacg catttgccgc gcgctgcccg
     4321 ccattttgcc gctcctaact ccgggccgtc gccaggtccg gcaggacgtc gcgcaagtcg
     4381 cgatgccccc gcactccggc cagataaccc ccgacgagcg gtcccggcaa ccgcaggccc
     4441 tccagaaccg cgaccagctc ctcgacgg
//
LOCUS       DASVLL010000191         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_333670,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000191 DASVLL010000000
VERSION     DASVLL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333670"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VF402_03165"
     CDS             complement(<1..994)
                     /locus_tag="VF402_03165"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7799321.1"
                     /translation="MVRRVVITGLGLVTPLGAGVEVSWKRLIDGQSGAGPITAFDASD
                     YACQVACEIPSVDGRGGGTPDMEGVFDPSLVLSNKERRKVDDFILYAIAAADEALNDA
                     DWHPESEEDQERTGVMIGSGIGGLGVIAETALEMQAKGHKRISPFFIPSALINLASGQ
                     VSIRHKLKGPNHAVVTACATGAHAIGDAARLIRSGDADVMVAGGAESSIVPIGIAGFI
                     ACRALCTAFNDNPQAASRPYDKDRDGFVMGEGAGIVVLEDYEHARARGAKIYGEVKGY
                     GLSGDAFHITAPSEDGDGGFRAMQMALKKSGFAPSDIDYVNAHGTSTMADYIEAG"
     gene            complement(1085..1324)
                     /locus_tag="VF402_03170"
     CDS             complement(1085..1324)
                     /locus_tag="VF402_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7799322.1"
                     /translation="MSDVLERVRKIVIDHLDADPDKVTEKASFIDDLEADSLDIVELV
                     MAFEEEFDIEIPDDSAEHILTVGDAVNYINEKLKA"
     gene            complement(1496..>2176)
                     /gene="fabG"
                     /locus_tag="VF402_03175"
     CDS             complement(1496..>2176)
                     /gene="fabG"
                     /locus_tag="VF402_03175"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX7799323.1"
                     /translation="RAIASTLHAQGAHVVLSGTRREALDALAADLGERTSVAPCDLGD
                     GAAVDALVGQAEAGAAPIDILVSNAGLTRDGLILRMKDEDWETVIKVNLEAYFRLARA
                     ATKGMMKRRHGRIIGVTSIVGVTGNPGQTNYAASKAGMIGFSKALAQEVGSRGITVNC
                     IAPGFIASPMTDELNDAQRAAILAKIPAGDLGKGADIAAAAIYLASAEAGYVTGQTLH
                     VNGGMAMI"
BASE COUNT          371 a          719 c          685 g          401 t
ORIGIN      
        1 caccggcctc gatatagtcc gccatggtgg acgtgccgtg ggcgttgacg tagtcgatgt
       61 ccgacggcgc gaagccggat ttcttgagcg ccatctgcat ggcgcggaag ccgccgtcgc
      121 cgtcttccga cggcgcggtg atgtggaagg cgtcgccgga aaggccgtag cccttcacct
      181 cgccgtagat cttggcgccg cgcgccctgg cgtgttcgta gtcctccagc acgacgatgc
      241 ccgcgccctc gcccatgacg aagccgtccc ggtccttgtc ataggggcgc gacgccgcct
      301 gcggattgtc gttgaaggcg gtgcagagcg cgcggcaggc gatgaagccc gcgatgccga
      361 tcggcacgat cgacgattcg gcgccgcccg ccaccatcac gtcggcgtcg cccgaacgga
      421 tcagccgggc cgcgtcgccg atggcgtgcg cgccggtggc gcaggccgtc accaccgcat
      481 ggttcgggcc tttgagttta tgacggatcg acacctgacc ggaagccaga ttgatcaggg
      541 ccgagggaat gaagaagggc gagatgcgct tgtggccctt ggcctgcatc tccagcgcgg
      601 tctcggcgat cacgcccaga cccccgatgc cggagccgat catcacgccg gtgcgctcct
      661 gatcctcttc gctttcggga tgccagtccg cgtcgttcag cgcttcgtcc gccgccgcga
      721 tggcgtacag gatgaaatcg tcgaccttgc gccgctcctt gttggacagc accagagacg
      781 ggtcgaacac gccttccatg tcgggcgtcc cgccgccgcg tccgtcgacg ctggggattt
      841 cacaggccac ctgacaggcg tagtcgctgg cgtcgaaagc tgtgatcgga ccggcgcccg
      901 actgaccgtc gatcagccgt ttccaggaaa cctcgactcc cgcgcccagg ggcgtaacca
      961 gacccaaacc cgtaatgacg acgcgacgaa ccatgcttac ccctgtcgtt cagccctaaa
     1021 gaaaccgagc gcggccagac ccccggcggg agcctgaaaa ccgcgctcgc gcaaaagctt
     1081 tcgcttaggc cttcagcttt tcgttgatat agttgacggc gtcgccgacc gtcaggatgt
     1141 gctcggcgga gtcatccgga atttcgatat cgaattcttc ttcgaaagcc atgaccagtt
     1201 cgacgatgtc gagcgaatcg gcttccagat cgtcgatgaa gctggccttt tcggtcacct
     1261 tgtccggatc ggcgtccagg tggtcgatca cgatcttgcg aacacgttca agaacatcag
     1321 acatttatag tcccttcaga cgttttaact atcccaaacc cctgacccgc aaaggggctt
     1381 ggaggggtca tcagaatcca gatttggtct tgtaggcaca gatgcgcctt gtttcaagcc
     1441 atttcaagcg cgccggacag actcttgcgt tacgcaaaag catgcgggcc gcaaatcaga
     1501 tcatcgccat gccgccgttg acgtgcaggg tctgaccggt gacgtaaccc gcttcggcgc
     1561 tggccagata gatcgccgcc gccgcgatgt cggcgccctt gccgaggtcg cccgccggga
     1621 ttttcgccag aatcgccgcg cgctgcgcgt cgttgagttc gtccgtcatc ggcgaggcga
     1681 tgaagcccgg cgcgatgcag ttgaccgtaa tgccgcgcga accgacctcc tgcgccagcg
     1741 ccttggaaaa gccgatcatg cccgccttgg aggcggcgta attggtctgg cccggattgc
     1801 cggtcacgcc gacgatcgag gtgacgccga tgatgcggcc gtggcggcgc ttcatcatgc
     1861 ctttcgtggc cgcgcgcgcg aggcggaaat aggcttcgag attgaccttg atgaccgtct
     1921 cccagtcctc gtccttcatg cgcaggatca ggccgtcgcg cgtcaatcct gcgttggaca
     1981 ccaggatgtc gatgggcgcg gcgccggcct cggcctggcc gacgagtgca tccaccgccg
     2041 ccccatcacc caggtcgcag ggcgccaccg atgtccgttc gcccagatcc gcggctaggg
     2101 cgtccagcgc ctcgcgccgc gtgccggaca agacgacgtg cgcgccctgc gcgtgcaggg
     2161 tggaggcgat ggcccg
//
LOCUS       DASVLL010000192         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_11681, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000192 DASVLL010000000
VERSION     DASVLL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11681"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..2054)
                     /locus_tag="VF402_03180"
     CDS             complement(33..2054)
                     /locus_tag="VF402_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX7799324.1"
                     /translation="MSQSAPAQTRPVRHVEFTVRGVVLGILITLVFTAAQVYLGLKVG
                     LTFATSIPAAVISMALLQAFRNASIQENNIVQTIASAAGTLASVIFVLPGLMIIGWWR
                     DVHLVTSFAACAIGGVLGVMYTMPLRRALVSQSSLPYPEGVAAAEVLRVGTSSREGGA
                     EGRAGLWAVIWGSLASALYAALAGAKLLAAEISVYFKVGSKGATGLAGSSSLALIGAG
                     HLMGITVGVAMFVGLALAWGVFVPVLSLHFPMPDLSAADHAGAVWSTKVRFLGAGAIG
                     AAAIWTLGKLALPVWSGLMSALETSRLSRTQGAVTPRTEQDLPVWIVGLVVLISMIPA
                     GWLLMSFVSGGPLNDLALPLVVTGVLYILIAGLLAAAVCGYMAGLIGSSNSPVSGIAI
                     LTVIGAAFITGMIGYKFTGPDTGQALIAFALLVTTVVLAVAVIGNDNLQDLKTGQLVD
                     ATPWKQQVALIIGILAGSLVVPPVLELLNNSNGFAGDPHYHAITQPPLPAPQATLIST
                     LAKGVIGGDLDWGLLGIGALVGLGLVVADIVLRKASKDRLSLPPLGVGLAIYLPSAVT
                     APVVVGAVAGWLFEKLVTKRGQNWGEAAKRLGVLVASGFIVGESLLNVALAGIIVATG
                     DAAPFAGPFKAFAPSEGVSMLIALVLAFIGVAGLYRWAAARAKKVTAGA"
     gene            complement(2150..2557)
                     /locus_tag="VF402_03185"
     CDS             complement(2150..2557)
                     /locus_tag="VF402_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799325.1"
                     /translation="MIRTVRLVFAAAALTGLLLAPVAFAQDDDGGDDDGGDDAVMAAA
                     IGCVATYDLVLARGLAGSHVAAVQKSRAQAREIYKEAAGLDDDAADADIAQADARLPS
                     LLEDGNASLQKYRHICDEMLSDDDEDAPQATSA"
     gene            complement(2621..>2941)
                     /locus_tag="VF402_03190"
     CDS             complement(2621..>2941)
                     /locus_tag="VF402_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7799326.1"
                     /translation="IAEDFTAAYQKCDVILTPATPSAAFELGDQAKAADPVQMYLNDV
                     FTVTTNLAGLPGMSVPAGLDKNGLPLGLQVIGKALDEATVLNVGQAIERAAGFTARPN
                     RWWA"
BASE COUNT          504 a         1042 c          983 g          412 t
ORIGIN      
        1 cccgctttag aaaagcgccc ggaggcgttt tttcacgccc ccgccgtcac ctttttggcg
       61 cgcgccgccg cccagcggta cagcccggcg acgccgatga aggccaagac cagcgcgatc
      121 agcatcgaga cgccctcgga cggcgcgaaa gccttgaacg gaccggcgaa cggcgcggcg
      181 tcgccggtgg cgacgatgat cccggccaga gcgacgttga gcaggctttc gccgacgatg
      241 aagcccgacg cgaccagcac gccgagccgc ttggccgcct cgccccagtt ctggccgcgc
      301 ttcgtcacca gcttctcgaa cagccagccc gccacggcgc cgaccaccac gggcgcggtc
      361 acggcggacg gcaggtagat ggccagcccc acccccagcg gcggcaggct gagcctgtcc
      421 ttgctggcct tgcgcagaac gatgtcggcg accacgaggc ccagcccgac cagcgcgccg
      481 atccccaaca gtccccagtc gagatcgccg ccgatcaccc ccttggccag ggtcgagatc
      541 agcgtcgcct gcggcgcggg caacggtggc tgggtgatgg cgtgatagtg cggatcgccc
      601 gcgaagccgt tggagttgtt gagcagctcc agcaccggcg gcacgaccag cgacccggcc
      661 aggatgccga tgatcagcgc gacctgctgc ttccacggcg tcgcgtcgac cagttgcccg
      721 gtcttgagat cctgaagatt gtcgttgccg atcaccgcca cggccagcac caccgtggtg
      781 accagcagcg cgaaggcgat cagcgcctgc cccgtgtcgg gaccggtgaa cttatagccg
      841 atcatgccgg tgatgaaggc cgcgccgatc acggtcagga tggcgatgcc cgacaccggc
      901 gagttggacg agccgatcag cccggccatg tagccgcaca ccgccgccgc cagcagcccg
      961 gcgatcagga tgtacagcac gccggtgacg accagcggca gggccagatc gttgagcggc
     1021 ccgccggaca cgaagctcat caaaagccag cccgccggga tcatgctgat cagcacgacg
     1081 aggccgacga tccacaccgg caggtcctgc tcggtgcgcg gcgtgacggc gccttgcgtg
     1141 cggctcaggc ggctggtctc cagcgccgac atcaggcccg accataccgg cagggccagc
     1201 ttgcccagcg tccagatcgc cgccgcgccg atggcgcccg cgccgaggaa gcgcaccttg
     1261 gtcgaccaga ccgcgcccgc atggtccgcg gcggacaggt ccggcatggg gaaatgcagg
     1321 ctgaggaccg gcacgaacac gccccacgcc agggccagcc cgacgaacat ggcgacgccg
     1381 accgtgatgc ccatcagatg accggcgccg atcagggcca gcgaggacga accggcgagg
     1441 cccgtcgcgc ccttgcttcc gaccttgaaa tagaccgaga tttccgccgc cagcagcttg
     1501 gcgcccgcca gcgcggcgta gagcgccgaa gccaggctgc cccagatcac cgcccacaga
     1561 ccggcgcggc cctccgcccc gccttcgcgc gacgaggtgc cgacgcgcag cacctcggcg
     1621 gcggcgacgc cttcgggata cggcagcgac gactggctga ccaaggcgcg gcgcagcggc
     1681 atggtgtaca tcacgcccag cacgccgccg atggcgcagg cggcgaagct ggtgaccaga
     1741 tgcacgtcgc gccaccagcc gatgatcatc agccccggca gcacgaagat caccgaggcc
     1801 agcgtgcccg ccgccgaggc gatggtctgg acgatgttgt tttcctgaat cgaggcgttc
     1861 ctgaacgcct gcaacagcgc catcgagatc accgccgccg ggatcgaggt ggcgaaggtc
     1921 agcccgacct tgaggcccag atagacctgc gcggcggtga agaccagcgt gatcaggatg
     1981 ccgagcacga cgccgcgaac ggtgaattcg acgtgtctga cggggcgggt ctgggcgggc
     2041 gcggactggc tcatgaagga ctccggtgga tcgctggccc tgatttagcc gcaagtcgcg
     2101 gcgttcgcca agatgtttgt tgcaaaagat atttttgcgc cgtccccggt cacgcgctcg
     2161 tcgcctgcgg cgcgtcctcg tcgtcgtcgc tcagcatctc gtcgcagata tggcggtatt
     2221 tttgcaggct ggcgttaccg tcctccagca gcgacggcag ccgcgcgtcg gcctgggcga
     2281 tgtcggcgtc cgccgcgtcg tcgtcgaggc ccgccgcttc cttgtagatt tcgcgcgcct
     2341 gcgcccgcga tttctggacc gcggcgacat ggctgccggc cagaccgcgc gccagcacca
     2401 gatcgtaggt cgccacgcag ccgatggcgg cggccatgac cgcgtcgtcg ccgccatcgt
     2461 catcgccgcc gtcatcgtcc tgcgcgaagg cgacgggcgc caggagcagc ccggtcaggg
     2521 cggcggcggc gaaaacgaga cggactgtgc ggatcatgac gcgcccttgc gaaaacggat
     2581 cacaggtaac gccgcgcggc gattccggca agacgcggct ttacgcccac catctgttcg
     2641 gacgcgccgt gaaaccggcg gcgcgctcga tcgcctgacc gacgttcagc accgtggctt
     2701 cgtccagcgc cttgccgatc acctgcaagc ccagcggcag gccgttcttg tccagaccgg
     2761 cgggcacgct catgcccggc agaccggcca aattggtggt gaccgtgaac acgtcgttca
     2821 ggtacatctg caccggatcg gcggccttgg cctgatcgcc cagctcgaag gcggcggacg
     2881 gcgtggccgg ggtcaggatc acgtcgcact tttgataggc cgccgtgaaa tcctcggcga
     2941 t
//
LOCUS       DASVLL010000193         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_280438,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000193 DASVLL010000000
VERSION     DASVLL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280438"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..>2578)
                     /locus_tag="VF402_03195"
     CDS             complement(185..>2578)
                     /locus_tag="VF402_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEX7799327.1"
                     /translation="AVLDNRAAPNAFSSELDLSLPDAPLPQGWSLYFGMVNAVKAMDS
                     DAFDIAHVNGDLYRLTPKPGAALRPGQTYAIRLTAEGHYYSRFHALPNAYIAADGLLP
                     RVIAATKPVYDPATKMEDLPFVAPMTDAAKLEVASPQDQTRWLTPQRAFAENASHQAD
                     ADAPGIIVLPTPVSARHLAGAALDLGRGVNLSLSGLTRADVAAALRDGGWRESAHGVP
                     LSLSVTGQGAPESYSLKAEDGRILIKAADAAGAFYALESLAQQAAFEHQRLKPLEIED
                     APRFAFRGLHLDLARNFESKAQILKIIAVMGQEKLNKLHLHLGDDEGWRLAIPGLPEL
                     TEIGAARCHDLAENHCLLPELGSGPQNTGGVNGYLTRDDYIEILQAAKARHIEVIPSF
                     DMPGHSRAAIRSMEARARRLIAAGQPEAAAEYRLYDPDDKTVYDSIQHYDDNTLNVCM
                     PSTYRFIGHVIDAIKAMHDAAGEPLRIYHIGTDETAGAWKDSPLCQKVMADEHLTPEQ
                     LGQRFIVKVAKMLADKGIEPAGWSDGMGAVDPAQLPRVVQSNAWGGLFTGGAMDAQKQ
                     ANQGWNVVLSTPDVLYLDMPYAPDPNERGYDWASRQTPVFKLFAFMPDNLPANASIMK
                     DTLDRPAKIVDDEPLTDGHRVAGMQGQLWSETVRSPLIADYMLFPRVEALSERAWRKA
                     DWEPDYKPEQSYAYGDGQVNAAKLNADWSAFNVKLNARLAALDAEGIVYRLPPPGGRI
                     TGGVLEANVAYGDLVVEYRETGGGWKRYYGAVAVKAPVALRSVSPDGRRVSRTVSIQ"
BASE COUNT          378 a          903 c          856 g          441 t
ORIGIN      
        1 gacgacgaac tgtacgacga gccgccgccg cctgagcctc cgccgcccga tccgccgccg
       61 caagccgatg tcgcgctgat cagaacgacc agcgccgccg ccatgccggc ggcgcgcata
      121 tccttagcca tgttttcctc ccgcgccgtc cggcggcagg ccggcggcgg gccggcggcg
      181 attattattg gatgctgacc gtgcggctga cgcgtctgcc gtccggtgac accgaacgta
      241 gcgcaaccgg cgccttgacc gcaaccgcgc cgtaatagcg cttccagccg ccgcccgttt
      301 cgcgatattc gacgaccaga tcgccatagg cgacattggc ctccagcacg ccgccggtga
      361 ttcgtccgcc cggcggcggc aggcgataga cgatcccttc cgcgtccagc gcggccagcc
      421 gcgcgttcag cttgacgttg aaagcgctcc agtcggcgtt cagcttcgcc gcgttcacct
      481 gcccgtcgcc ataggcgtag ctctgctccg gcttgtagtc cggctcccag tccgccttgc
      541 gccatgcccg ctcgctcagg gcctcgacgc gcgggaacag catatagtcg gcgatcagcg
      601 gcgagcgcac cgtctcggac cacaactgcc cctgcatccc ggcgacgcga tggccgtccg
      661 tcagcggctc gtcgtcgacg atcttcgccg ggcggtccag cgtgtccttc atgatcgagg
      721 cgttggcggg caggttgtcc ggcatgaagg cgaacagctt gaacaccggc gtctggcgcg
      781 aggcccagtc atagccgcgc tcgttcggat cgggcgcgta gggcatgtcg agataaagca
      841 cgtccggcgt cgacaggacg acgttccagc cctgattggc ctgcttctgc gcgtccatgg
      901 cgccgccggt gaacagcccg ccccaggcgt tcgactgcac caccctgggc aattgcgccg
      961 gatcgaccgc gcccatgccg tcgctccagc ccgccggttc gatccccttg tccgccagca
     1021 tcttcgcgac cttgacgatg aaacgctggc ccaactgttc cggcgtcaga tgctcgtcgg
     1081 ccatgacctt ctggcagagc ggcgagtcct tccacgcgcc cgccgtttcg tccgtgccga
     1141 tatggtagat gcgcaaaggc tcgcccgccg cgtcgtgcat cgccttgatc gcgtcgatca
     1201 catggccgat gaagcggtag gtcgagggca tgcagacatt gagcgtgttg tcgtcgtaat
     1261 gctggatgct gtcatagacc gtcttgtcgt ccgggtcgta gaggcggtat tcggcggcgg
     1321 cctccggctg gcccgccgcg atcaggcggc gcgcgcgcgc ctccatcgaa cggatggcgg
     1381 cgcgcgaatg gccgggcatg tcgaaggacg gaatcacctc gatatggcgc gccttcgcgg
     1441 cctgcaaaat ctcgatatag tcgtcgcgcg tcagatagcc gttgacgccg cccgtgtttt
     1501 gcggcccgga gccgagttcc ggcagcaggc aatgattctc ggccagatcg tgacagcgcg
     1561 ccgcgccgat ctcggtcagc tccggcaggc ccggaatcgc cagccgccag ccctcgtcgt
     1621 cgccgagatg caggtgcagc ttgttgagct tttcctgccc catgacggcg atgatcttga
     1681 gaatctgcgc cttgctctcg aaattgcgcg ccagatcgag atgcaggccg cggaacgcaa
     1741 agcgcggcgc gtcctcgatc tccagcggct tcaggcgctg atgctcgaac gccgcctgct
     1801 gggccagcga ttccagcgcg taaaacgccc cggcggcgtc cgccgccttg ataaggatgc
     1861 gcccgtcctc agccttgagg ctgtaggact ccggcgcgcc ctgaccggtg acgctcaggc
     1921 tgagcggcac gccgtgcgcg ctttcgcgcc agccgccgtc gcgcagcgcc gccgccacgt
     1981 cggcgcgcgt cagcccgctc agggacaggt tcacgccccg gccgaggtcg agcgccgccc
     2041 ctgccagatg gcgcgcgctg accggggtgg gcaggacgat gatgccgggc gcgtccgcgt
     2101 ccgcctgatg cgaggcgttt tccgcgaagg cgcgctgcgg cgtcagccag cgcgtctggt
     2161 cctgcgggct ggcgacctcc agcttcgccg cgtcggtcat cggcgcgacg aacggcagat
     2221 cctccatctt ggtcgccgga tcgtaaaccg gcttcgtcgc cgcgatcacg cgcggcagca
     2281 ggccgtcggc ggcgatatag gcgttgggca gggcgtggaa gcgcgaataa taatgcccct
     2341 ccgccgtcag cctgatcgca taggtctggc cgggccgcag cgccgcgccg ggtttcggcg
     2401 tcaggcggta gaggtcgccg ttgacatgcg cgatgtcgaa ggcgtcgctg tccatcgcct
     2461 tgaccgcgtt gaccatgccg aaatagagcg accagccctg cggcagcggc gcgtcgggca
     2521 ggctcaggtc cagttcgctg ctgaaggcgt tgggcgcggc gcggttgtcc agcaccgc
//
LOCUS       DASVLL010000194         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_118910,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000194 DASVLL010000000
VERSION     DASVLL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118910"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF402_03200"
     CDS             <1..180
                     /locus_tag="VF402_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799328.1"
                     /translation="FYAKFRASRCAGAVVEPDSEMVRASRYAVGQVCFDHGKHGKHGK
                     KREALYLAAGGLRVI"
     gene            complement(238..1527)
                     /locus_tag="VF402_03205"
     CDS             complement(238..1527)
                     /locus_tag="VF402_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEX7799329.1"
                     /translation="MLIATFATLIPILLLILAVSALFSAGETSLTAVSRGRMHQLERD
                     GDRAARRVNRLLTDREKMIGAILLLQNVLNIGASAVATVSIDRVIPGPWGAAISTGLM
                     TVLVLVFGEVLPKTLAIMRPDDVARFLSRPVLWAVVLLGPIITAVQWFVRGTLRLFGV
                     KLSMEVDVLAAHEEIRGAVEYHHEEGAVRSEDRNMFRGVLDLADLDVSQIMVHRKSID
                     MIDADLSTAEIIEAALNFSHTRLPLYRDNTENIIGVLHARDLMAAITRAGGQTDKVDV
                     AAVLREPWFIPDTTNLKDQLAAFLKKKMHFALVVDEYGALQGLVTLEDILEEIVGEID
                     DEHDAVMNAIRRQGDGSVHVDGDLPIRDLNRYMDWELPDDHAVTVAGLVIHEARTIPE
                     PGQTFIFYDRKFQILRKQRNQITAIKITVVPRVQDED"
     gene            complement(1521..>1926)
                     /locus_tag="VF402_03210"
     CDS             complement(1521..>1926)
                     /locus_tag="VF402_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7799330.1"
                     /translation="GHTFGHALEAEVGFGDALLHGEAVAIGMCQAFRYSALSGECTEA
                     DVARAVRPIAHAGLPTKMSDIKWRNGRNAPFDAQRLIEHMSHDKKAEGGHLTFVLAGG
                     IGHAFVARKVDRASICDFLVMDGAILNEDAKC"
BASE COUNT          320 a          614 c          637 g          355 t
ORIGIN      
        1 ttttacgcga agttcagggc ttcgcgttgc gcaggcgccg tcgtagaacc ggattcggag
       61 atggtgcgcg cttcgcgata tgccgtcgga caggtttgtt ttgaccacgg aaaacacgga
      121 aaacacggaa aaaagcgcga agcgctctat ctggctgcgg gcggcttacg cgtgatttaa
      181 ggcgctgcgc gcaagaccta tgtccgtgtt ttccgtgttt tccgtggtca aaaatgctta
      241 gtcttcgtcc tgaaccctgg gcaccacggt gatcttgatg gcggtgatct ggttgcgctg
      301 tttgcgcagg atctggaact tgcggtcgta gaagatgaag gtctggcccg gctccgggat
      361 ggtgcgcgct tcgtggatga cgaggccggc cacggtgacg gcgtggtcgt cgggcagctc
      421 ccagtccatg tagcggttga ggtcgcggat cggcaggtcg ccgtcgacat gcaccgagcc
      481 gtcgccctgg cggcggatgg cgttcatgac ggcgtcgtgt tcgtcgtcga tctcgccgac
      541 gatctcctcc agaatgtcct ccagcgtgac gaggccctgc aacgcgccgt actcgtcgac
      601 caccagcgcg aagtgcatct tcttcttcag gaaggccgcc aactggtcct tgaggttggt
      661 ggtgtcgggg atgaaccacg gctcgcgcag caccgccgcg acgtcgacct tgtccgtctg
      721 gccgcccgcg cgggtgatcg ccgccatcag gtcgcgcgcg tgcagcacgc cgatgatgtt
      781 ctcggtgttg tcgcgataga gcggcaggcg ggtatggctg aaattcagcg ccgcctcgat
      841 gatctcggcg gtcgagaggt cggcgtcgat catgtcgatc gacttgcgat gcaccatgat
      901 ctggctgacg tcgaggtcgg ccaggtccag cacgccgcgg aacatgttgc ggtcctcgct
      961 gcgcacggcg ccttcctcat ggtggtattc gacggcgccg cggatttcct catgcgcggc
     1021 cagcacgtcg acctccatcg acagcttcac gccgaacagg cgcagcgtgc cgcgcacgaa
     1081 ccattgcacg gcggtgatga tcggccccag cagcaccacg gcccacaaaa ccggtcgcga
     1141 caggaagcgc gccacgtcgt cgggcctcat gatggccagc gtcttgggca gcacctcgcc
     1201 gaacaccagc accagcacgg tcatcagccc ggtcgagatc gccgcgcccc acggccccgg
     1261 aatcacccgg tcgatggaga cggtcgccac ggcggaggcg ccgatgttca gcacgttctg
     1321 caacagcagg atcgcgccga tcatcttttc gcggtcggtc aaaagccggt tgacccggcg
     1381 cgcggcgcgg tcgccgtcgc gctccaactg gtgcatccgc ccgcgcgaca cagcggtcag
     1441 cgacgtctcg cccgcgctga acaaggccga caccgccaga atcagcagca ggatcgggat
     1501 cagggtggcg aatgtggcta ttaacatttt gcatcctcgt tgaggatggc gccgtccatc
     1561 accagaaaat cgcagatcga ggcgcggtcg accttcctgg cgacgaaggc atggccgatg
     1621 ccgccggcca gcacgaaggt cagatgcccg ccctcggcct tcttgtcgtg gctcatatgc
     1681 tcgatcaggc gttgcgcgtc gaacggcgcg ttgcggccgt tgcgccattt gatgtcgctc
     1741 atcttcgtcg gcagaccggc gtgggcgatg gggcggacgg cgcgcgccac gtccgcctcg
     1801 gtgcattcgc cgctcagggc gctgtagcgg aaggcctgac acatgccgat ggcgaccgcc
     1861 tcgccgtgca gcagcgcgtc cccgaagccg acctcggcct ccagcgcgtg accgaaggtg
     1921 tggccg
//
LOCUS       DASVLL010000195         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_64731, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000195 DASVLL010000000
VERSION     DASVLL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64731"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..526)
                     /locus_tag="VF402_03215"
     CDS             complement(236..526)
                     /locus_tag="VF402_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX7799331.1"
                     /translation="MPKAYLIAQASVSDPDAYARYVAGTKAAFEKYGARPLARGGRTE
                     VMEGQGRPRVVVLEFADLDTARAYYHSAEYQAAKQERLGAADFDSIIVEGVA"
     gene            623..1456
                     /gene="dapF"
                     /locus_tag="VF402_03220"
     CDS             623..1456
                     /gene="dapF"
                     /locus_tag="VF402_03220"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7799332.1"
                     /translation="MTRAFLKMNGLGNDFIVVDAREAAFAPAPDQIRAWGDRASGIGF
                     DQLISIEKSDAGDAFMRVWNSDGGTVETCGNALRCVAWVLNRDAADRTLKIDTLGGAT
                     RARVLRADATTGTASVDMGRPRLNWREIPLSEEMDTLRLELKVGPYDAPLYHTPCAVS
                     MGNPHVVFFVDDVEAVDVAATGSLIENHPLFPEGVNVEFAQVIDRNTIRMRVWERGAG
                     ITKACGTGACATLVACARRGLTDRAAAVIMDGGPLHIQWADNDHVFMTGPVEVEFEGA
                     L"
     gene            1453..>2190
                     /locus_tag="VF402_03225"
     CDS             1453..>2190
                     /locus_tag="VF402_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7799333.1"
                     /translation="MSPKNQDKLDIVTFGCRLNSYESEVIRKTATEDGLSNAIIFNTC
                     AVTGEAVRQARQAIRRARKENPDARLIVTGCAAQTDPDTFAQMAEVDFVIGNADKQKK
                     GAYALTPDSARVVVNDVFSVKETAGHLIDGLKDRARAFVEVQNGCDHRCTFCIIPYGR
                     GNSRSAAAGDVIAQTQKLVAEGYNEVVLTGVDLTSWGADLPGTPQLGHLVARILKLVP
                     DLKRLRLSSIDAAEIDDALLNVLRDEER"
BASE COUNT          392 a          729 c          707 g          362 t
ORIGIN      
        1 tttcggcgcc ttcttcaagt tcgtcgaagc ggcggcggtc aacgacgccg acgccatctc
       61 cgtgcgctcc gatcccaatg gcgaccatca ggtcaaggtc gtgaccttcg aggacgcgtg
      121 tcaggccgac cagttcaaga cctactggac gcagcgccgc cgctggctgg ggctttagag
      181 cgctttccga aaaatgtgac gcacttttcg gattaaaaag cgctctaacc cccgctcacg
      241 ccacgccttc gacaataatc gaatcgaagt ccgccgcgcc gagacgctcc tgtttcgccg
      301 cctgatattc ggcggaatga taataggcgc gggccgtatc cagatcggcg aattccagca
      361 ccaccacgcg cggacggccc tgtccctcca tcacctccgt gcgtccgccg cgcgccagcg
      421 gccgggcgcc gtatttttcg aacgcggctt tcgtaccggc gacatagcgc gcataggcgt
      481 cgggatcgga cacgctggcc tgcgcgatca ggtaggcttt gggcatggga aggctccttg
      541 tctgggcgcc ccttcatagc cttgcggcat tttgcggaaa agcctgtaag tttcgcggct
      601 atgcgactat atacgcaaaa tcatgacgcg cgcatttctg aagatgaacg gcctcggcaa
      661 tgatttcatt gtcgttgacg cccgcgaagc cgccttcgcg cccgcgccgg accagatccg
      721 ggcgtggggc gaccgcgcgt ccggcatcgg cttcgaccag ttgatcagca tcgaaaagag
      781 cgatgcgggc gacgccttca tgcgcgtgtg gaacagcgac ggcggcacgg tcgaaacctg
      841 cggcaacgcg ctgcgctgcg tggcgtgggt gctgaaccgc gacgccgccg accgcaccct
      901 gaagatcgac acgctgggcg gcgcgacaag ggcgcgcgtg ctgcgcgccg acgcgacgac
      961 cggcacggcc agcgtcgaca tgggcaggcc gcgcctgaac tggcgcgaga ttccgctgtc
     1021 ggaggagatg gacacgctgc ggctggagct gaaggtcggg ccgtacgacg cgccgctcta
     1081 tcacacgccc tgcgccgtct cgatgggcaa tccgcacgtc gtctttttcg tcgatgacgt
     1141 cgaagcggtc gacgtcgcgg cgaccggctc gctgatcgaa aaccatccgc tcttccccga
     1201 aggcgtcaat gtcgagttcg cgcaggtgat cgaccgcaac acgatccgca tgcgcgtctg
     1261 ggagcgcggc gcgggcatca ccaaggcgtg cggcaccggc gcctgcgcca cgctggtcgc
     1321 ctgcgcgcgc cgcggcctga cggatcgcgc cgctgccgtc atcatggacg gcggtccgct
     1381 gcatattcag tgggcggaca acgaccacgt cttcatgacc ggccccgtcg aggtcgagtt
     1441 cgagggcgcg ttgtgagccc caaaaaccag gacaagctcg atatcgtcac cttcggctgc
     1501 cgcctcaaca gctatgagag cgaggtcatc cgcaagaccg cgaccgagga cgggctgtcc
     1561 aacgccatca tcttcaacac ctgcgccgtc accggcgaag cggtgcgtca ggcgcgtcag
     1621 gccatccgcc gcgcgcgaaa agagaatccc gacgcccggc tgatcgtcac cggctgcgcg
     1681 gcgcaaaccg acccggacac gtttgcgcag atggccgagg tcgatttcgt catcggcaac
     1741 gccgacaagc agaaaaaggg cgcctatgcc ctgaccccgg acagcgcgcg cgtcgtggtc
     1801 aacgacgtgt tctcggtgaa ggagacggcg ggccacctga tcgacggatt gaaggatcgc
     1861 gcccgcgcct ttgtcgaggt gcagaacggc tgcgaccacc gctgcacctt ctgcatcatc
     1921 ccctacgggc gcggcaattc gcgctcggcg gcggcgggcg acgtgatcgc gcaaacgcag
     1981 aagctggtgg ccgagggtta taacgaggtc gtgctgaccg gggtggacct gacctcgtgg
     2041 ggcgccgacc tgcccggaac gccgcaactg ggtcatctgg tcgcgcgcat cctcaagctg
     2101 gtccccgatt tgaagcgcct gcgcctgtcg tccatcgacg ccgccgagat cgacgatgcg
     2161 ctgctcaacg tgctgcgcga cgaggagcgc
//
LOCUS       DASVLL010000196         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_280679,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000196 DASVLL010000000
VERSION     DASVLL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280679"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VF402_03230"
     CDS             <1..1174
                     /locus_tag="VF402_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX7799334.1"
                     /translation="PAIYDGKAFARDGIVFVSFNYRVGRFGFFAFPELTKEDADHGQL
                     GDYAYMDMIAALKWVKANIAAFGGDPDQVTIFGESAGGAAMHTLLTSPQARGLFNRAI
                     IESGGGRGNLMGPRYLRDDRPGRPSGENIGVAFARAHGVDGTGPEALKALRALPADAL
                     VGGLGLADYQPDTYAGPMLDGHIVVEDPDKAYAAGHDAGVSVMVGANSGDLMPQSAAS
                     KDALFAAFGADADAARKVYDPDGAAPLARLNAEVGMDRLMVEPARLTARLLSAHGRPV
                     YEYRFDYTASQKKAGAPFGAPHASELPFVFETVDARYGEAATAQDHAASRLMHAYWAN
                     FAKTGDPNGAGLPQWPVYTADKDDLLIFTQDAGAGAVADPWKARLDVTAKAASEQH"
     gene            complement(1160..>1698)
                     /locus_tag="VF402_03235"
     CDS             complement(1160..>1698)
                     /locus_tag="VF402_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799335.1"
                     /translation="QATPQAAPAPLPPPVAVGDVPAPVASLPVQPLSAPPPPEPQNPP
                     QSSPQALAEISLKCETRVTAGRRVVSQGTFFIDLFPSPVFPDQQADFKFLFVDPAHKS
                     LIRDSICLDATCSATVSGAAYALVARRTRHGEALRITLDRSNGAFYAEEIDKGMTGGA
                     HLGEQGWCAPQKLPSVLF"
BASE COUNT          264 a          577 c          605 g          252 t
ORIGIN      
        1 gcccgccatc tatgacggca aggcgttcgc ccgcgacggc attgttttcg tcagcttcaa
       61 ctaccgcgtc gggcgcttcg gcttcttcgc cttcccggaa ctgaccaagg aggacgccga
      121 tcacggccag ctcggcgact acgcctatat ggacatgatc gcggcgctga aatgggtgaa
      181 ggccaatatc gcggccttcg gcggcgatcc cgatcaggtg accatcttcg gcgaatcggc
      241 gggcggggcg gcgatgcaca ccctgctgac ctcgccgcaa gccagggggc tgttcaaccg
      301 cgccatcatc gaatcgggcg gcgggcgcgg caatctgatg gggccgcgtt acctgcgcga
      361 cgacaggccg ggccgtccgt ccggcgaaaa catcggcgtc gccttcgcgc gggcgcacgg
      421 cgtcgacggg accggaccgg aggcgctgaa ggcgttgcgc gccctgcccg ccgacgccct
      481 ggtcggcggc ctcggccttg ccgactatca gccggacacc tatgccgggc cgatgctgga
      541 cggccatatc gtcgtcgaag acccggacaa ggcctatgcc gccggacacg acgccggcgt
      601 ctccgtcatg gtcggcgcca acagcggcga cctgatgccg caatccgccg ccagcaagga
      661 cgccttgttc gccgccttcg gcgccgacgc cgacgcggcg cgcaaggttt acgacccgga
      721 cggcgcagcg ccgctggccc ggctcaacgc cgaggtcggc atggatcggc tcatggtgga
      781 gcccgcgcgc ctgaccgcgc ggcttctgag cgcgcacggc cgtcccgttt acgaataccg
      841 cttcgactat acggcctcgc aaaagaaggc gggcgcgcct ttcggcgccc cgcacgccag
      901 cgagttgccc ttcgtgttcg agacggtgga cgcgcgctac ggcgaggcgg cgacggcgca
      961 ggatcatgcg gcttcgcgcc tgatgcacgc ctactgggcc aatttcgcca agaccggcga
     1021 tccgaacggc gcgggcctgc cgcaatggcc ggtctatacg gcggacaagg acgacctgct
     1081 gatcttcacc caggacgcgg gcgcgggtgc cgtcgccgat ccgtggaagg cgcggctcga
     1141 cgtgacggcg aaggcggcgt cagaacagca ctgacggcag tttctgcggc gcgcaccagc
     1201 cctgttcgcc cagatgcgcg ccgcccgtca tgcccttgtc gatctcctcg gcgtagaagg
     1261 cgccgttgct gcggtccagc gtgatgcgca gcgcctcgcc gtgccgggtg cggcgggcga
     1321 ccagcgcata ggccgcgccg gacaccgtgg ccgagcaggt cgcatcgagg cagatcgaat
     1381 cgcggatcag cgatttatgc gccggatcga cgaacaggaa tttgaagtcg gcctgctggt
     1441 ccgggaagac cggcgacggg aacaggtcga tgaagaaggt tccctgcgag acgacgcgcc
     1501 tgcccgcggt gacgcgggtt tcgcatttca ggctgatttc ggcgagagcc tgcggcgaac
     1561 tttgcggcgg attttgcggt tccggcggcg gcggcgcgct cagcggctgg accggcaggg
     1621 acgcgacggg cgccggaacg tcgccgaccg ccacgggcgg cggcagcgga gcgggcgccg
     1681 cttgcggagt cgcttgcg
//
LOCUS       DASVLL010000197         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_333988,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000197 DASVLL010000000
VERSION     DASVLL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333988"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VF402_03240"
     CDS             <1..458
                     /locus_tag="VF402_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7799336.1"
                     /translation="MLTLLGARAESVAGVDLSQHMLNIARAKTFEAGLTGADLRHGDI
                     YDTRLPAASADLIVVHQVLHFLADPGRAVLEAGRLLAPGGRLLIVDFAHHDLEVMREQ
                     YQHRRLGMLDEDMAAWLRAAGLMPRTSLALPPQGEGGLTVKIWLASRPA"
     gene            486..1415
                     /gene="metF"
                     /locus_tag="VF402_03245"
     CDS             486..1415
                     /gene="metF"
                     /locus_tag="VF402_03245"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX7799337.1"
                     /translation="MSPVLNPQLADLAPIARSLAGGGQNLNVSFEFFPPKTEEMEATL
                     WDSIRRLAPLNPSFVSVTYGAGGSTRERTHRTVKRILDETRLKPAAHLTCVDASRDEV
                     DEVIRAYRDAGVRHIVALRGDPPSGFGDYTPREDGYKNATELTAAIRRIGDFEVSIGV
                     YPEKHPQSPSQAFDLDVLRQKVDAGATRGISQFFFEPETYLRYRDAVADAGIGIDLTP
                     GIMPVTNFKGLRRMAAACAAHIPDWLEALFDGLDNDAETRKLLASAVAVELCLGLERE
                     GVETFHFYTLNRADLVYAICRILGLKETRHVES"
     gene            1402..>1790
                     /locus_tag="VF402_03250"
     CDS             1402..>1790
                     /locus_tag="VF402_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX7799338.1"
                     /translation="MLRAERIEALKQAAKERILILDGSWGVMIQRRELDEADFRAERF
                     KTYPGQLKGNNDILCITRPDIIADLHDQYYAAGADISETNTFSATTIAQDDYRLTLVD
                     VIDINREGAKIARAAADRWTQKEPGKPR"
BASE COUNT          309 a          598 c          584 g          299 t
ORIGIN      
        1 ggatgctgac ccttctgggg gcgcgcgccg aaagcgtggc gggcgtcgac ctgtcgcagc
       61 acatgctgaa catcgcgcgc gccaagacct tcgaggcggg cctgacgggc gccgacctgc
      121 gccacggcga catctacgac acgcgcctgc ccgccgcgtc cgccgacctg atcgtcgtgc
      181 atcaggtgct gcacttcctg gccgatccgg ggcgggccgt gctcgaagcc gggcggctgc
      241 tggcgccggg cgggcggctg ctgatcgtcg atttcgccca tcacgatctc gaagtcatgc
      301 gcgagcagta tcagcaccgc cgtctgggca tgttggacga ggacatggcg gcgtggctgc
      361 gcgccgccgg cctcatgccg cggacctcgc tggccctgcc gccgcaaggc gaaggcgggc
      421 tgaccgtcaa aatctggctg gcctcgcgtc cggcctaatt tcacttatat tttccgagcc
      481 ttatcatgag ccctgtgctg aatccccaat tggccgatct tgcgcccatc gcccgttcgc
      541 tggcgggcgg cggacaaaat ctcaatgtgt cctttgaatt cttcccgccc aagaccgagg
      601 agatggaggc gacgctttgg gactcgatcc gccgtctggc gccgctgaac ccgtccttcg
      661 tgtcggtgac ctacggcgcg ggcggctcga cgcgcgagcg cacgcaccgc acggtgaagc
      721 gcatcctcga cgaaacgcgc ctgaagcccg ccgcgcacct gacctgcgtc gacgcctcgc
      781 gcgacgaggt ggacgaggtg atccgcgcct accgggacgc gggcgtgcgg catatcgtgg
      841 ccctgcgcgg cgatccgcct tccgggtttg gcgactatac gccgcgcgaa gacggttata
      901 agaacgccac cgagctgacc gccgccatcc ggcgcatcgg cgatttcgag gtcagcatcg
      961 gcgtctatcc cgaaaagcac ccgcaaagcc cgtcgcaagc cttcgacctc gatgtgctgc
     1021 gccagaaggt cgacgcgggc gcgacgcgcg gcatcagcca gttcttcttc gagccggaaa
     1081 cctatctgcg ctatcgcgac gcggtggcgg acgcaggcat cgggatcgac ctgacgccgg
     1141 gcatcatgcc ggtgaccaat ttcaagggcc tgcgccgtat ggcggcggcg tgcgcggcgc
     1201 acattcccga ctggctggag gccctgttcg acggactgga caacgacgcg gagacgcgca
     1261 agctgctggc ctcggcggtg gcggtcgagc tgtgcctcgg ccttgagcgc gaaggggtcg
     1321 agactttcca tttctatacg ctcaaccgcg ccgatctggt gtatgcgatc tgccggattc
     1381 tgggtttgaa ggagacgcgc catgttgaga gctgaacgca tcgaagcctt aaaacaggcc
     1441 gccaaggagc ggattctgat cctcgacggc tcgtggggcg tgatgatcca gcgccgcgaa
     1501 ctggacgagg ccgatttccg cgcggagcgc ttcaagacct atccgggtca gctcaagggc
     1561 aataacgaca tattatgcat cacccggccg gacatcatcg ccgacctgca cgatcagtat
     1621 tacgcggcgg gcgcggacat ttccgagacc aacacctttt cggccaccac catcgcgcag
     1681 gacgactatc gcctgaccct ggtcgatgtg atcgacatca accgcgaggg cgcgaagatc
     1741 gcccgcgccg ccgccgaccg ctggacgcaa aaggagccgg gcaagccgcg
//
LOCUS       DASVLL010000198         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_441984,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000198 DASVLL010000000
VERSION     DASVLL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441984"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1601
                     /locus_tag="VF402_03255"
     CDS             <1..>1601
                     /locus_tag="VF402_03255"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEX7799339.1"
                     /translation="LVCDDFEAVADRYPTRPAIGFEGKTVTYQQLDTLANRYAHWARQ
                     RGLKAGDTVALFMPNRIDYIAIWLGLNKIGVITALINNSLSGAGLAHCINISMASLTL
                     VDTTTRAAFDEIEGQIERFQTLWVLDLPREDESEHCRSLDTALKGASTVRPDRALRAG
                     LTAGSVALYIYTSGTTGLPKAAKISNARAQLYMQAFAGLSHMKPEDKLYCVLPLYHAT
                     GGLCGVGAALMNGAEVVLKRRFSASQFWSDVRKEGVTHIVYIGELCRYLVNSPPAPNP
                     DDETKHKVRMAFGNGMRPEVWDHFKRRFKIPAIVEFYGSTEGNVSLFNLDGQPGAVGR
                     VPKILRSKFNVRLVRFDVETEMPIRGMNGLCQECKPGEIGEAIGVIGHDVRHSYSGYA
                     DKAATQKKILTDVLKKGDAWFRTGDLMRQDKHGYLYFVDRIGDTFRWKGENVSTSEVA
                     EYCASAPGVEEAIVYGVPVANHDGKAGMVALITREGFDLAAFKAHVDSHLPDFARPRF
                     VRLLSEVETTGTFKYKKMDLVSEGF"
BASE COUNT          291 a          563 c          504 g          243 t
ORIGIN      
        1 ctggtctgcg acgatttcga ggcggtggcg gaccgttacc cgacgcgccc cgccatcggc
       61 ttcgagggca agaccgtcac ctatcagcag ctcgacaccc tggccaaccg ctacgcccac
      121 tgggcgcgtc agcgcggcct caaggcgggc gacacggtgg cgctgttcat gcccaaccgc
      181 atcgactaca tcgccatctg gctggggctg aacaagatcg gcgtgatcac cgccctgatc
      241 aacaattcgc tcagcggcgc cggcctcgcc cactgcatca acatctcgat ggccagcctc
      301 accctggtgg acacgacgac ccgcgccgcc ttcgacgaga tcgagggcca gatcgagcgc
      361 ttccagaccc tgtgggtgct ggacctgccg cgcgaggacg aaagcgagca ctgccgcagc
      421 ctcgacaccg cgctcaaggg cgcctcgacc gtgcgccccg accgcgccct gcgcgcgggc
      481 ctgaccgcgg gctcggtcgc gctctacatc tacacctccg gcacgaccgg cctgcccaag
      541 gcggcgaaaa tctccaacgc gcgcgcccag ctttacatgc aggcgttcgc gggcctcagc
      601 cacatgaagc cggaggacaa gctgtactgc gtgctgccgc tgtaccacgc caccggcggg
      661 ctgtgcggcg tcggcgcggc gctgatgaac ggcgccgagg tcgtgctcaa gcgccgcttc
      721 tcggccagcc agttctggag cgacgtgcgc aaggaaggcg tgacccacat cgtctatatc
      781 ggcgagctgt gccgctatct cgtcaactcg ccgcccgcgc cgaacccgga cgacgaaacg
      841 aagcacaagg tgcgcatggc cttcggcaac ggcatgcgcc cggaggtgtg ggaccatttc
      901 aagcgccgct tcaaaatccc ggccatcgtc gaattctacg gctcgaccga gggcaatgtc
      961 tcattgttca acctcgacgg ccagcccggc gcggtcgggc gcgtgcccaa gatcctgcgc
     1021 agcaagttca acgtccgcct cgtgcgcttc gacgtcgaga ccgagatgcc gatccgcggc
     1081 atgaacggcc tgtgccagga atgcaagccc ggcgagatcg gcgaggccat cggcgtcatc
     1141 ggccacgacg tgcgccattc ctattccggc tacgccgaca aggccgcgac gcagaagaag
     1201 attctcaccg acgtgctgaa aaaaggcgac gcgtggttcc gcaccggcga cctgatgcgt
     1261 caggacaagc acggctacct ctatttcgtc gaccgcatcg gcgacacctt ccgctggaag
     1321 ggcgagaacg tctcgacctc ggaggtggcg gaatactgcg cgtccgcgcc gggggtcgag
     1381 gaggccatcg tctacggcgt gccggtcgcc aaccacgacg gcaaggcggg catggtcgcg
     1441 ctgatcacgc gcgaaggctt cgatctggcc gcgttcaagg cgcacgtcga ttcccacctg
     1501 cccgacttcg cgcgcccgcg cttcgtgcgc ctgctcagcg aggtcgagac gaccggcacc
     1561 ttcaagtaca agaagatgga tctggtcagc gagggcttcg a
//
LOCUS       DASVLL010000199         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_173499,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000199 DASVLL010000000
VERSION     DASVLL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173499"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="pseC"
                     /locus_tag="VF402_03260"
     CDS             complement(<1..1048)
                     /gene="pseC"
                     /locus_tag="VF402_03260"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HEX7799340.1"
                     /translation="MSFLPYGRQSIDDADIAAVVAALKSDFLTTGPLVERFEQALAQA
                     VGAQEAVVVANGTAALHLAVLSEDLGPQDVAVVPAVTFAASANCVAYCGARVVFADVD
                     PLTGLMTDESFNDALNLIARDHPDHRFRGVIPVHYAGRALDLGHIAEVAGTHGAFVIE
                     DACHALGSVGAQGPVGSCAISDMTCFSFHPVKTVTSGEGGAITLNDPQRAARLRRLRS
                     HGVERDPAHFTGDARGPWVYEMQELGYNYRLPDLNCALGLSQLARLDAFVARRKKLVG
                     AYLKALGASNLPVRWIAPDHDPALHLFAVSIDFASLGVTREAMMNGLRARGIGSQVHY
                     IPVPDMPYWRTRALG"
     gene            complement(1045..>1728)
                     /locus_tag="VF402_03265"
     CDS             complement(1045..>1728)
                     /locus_tag="VF402_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7799341.1"
                     /translation="TGAENVVWACLHNRVARVIALSTDKACSPVNLYGATKLASDKIF
                     VAANNLAGDIGTRFSVVRYGNVVGSRGSVAPYFKRLLENGARELPITDPRMTRFWISL
                     DQGVAFVLSSLDLMQGGEIFVPKIPATLVTDLARALAPGAAHRIVGVRPGEKLHEMMI
                     SADDARATVELDDRYVIEPEFVEYQRKSFRAAAGARTVEEGFSYASDNNRERLDAEGI
                     RAMMDAYLS"
BASE COUNT          282 a          564 c          625 g          257 t
ORIGIN      
        1 gtcccagcgc gcgcgtccgc caatagggca tgtccggcac ggggatatag tggacctgcg
       61 agcctatgcc gcgcgcgcgc aggccgttca tcatcgcctc gcgcgtcacg ccgaggctgg
      121 cgaagtcgat ggacacggcg aacaggtgca gcgccggatc gtggtcgggc gcgatccagc
      181 gcaccggcag gttggacgcg cccaaggcct tgagataggc gccgaccagt tttttgcgcc
      241 gcgcgacgaa ggcgtccagc cgcgccagtt gcgacaggcc gagcgcgcag ttcaggtcgg
      301 gcaggcggta attatagcca agctcctgca tctcatagac ccacgggccg cgcgcatcgc
      361 cggtgaagtg cgccggatcg cgctccacgc cgtgcgagcg caggcggcgc aggcgggcag
      421 cgcgttgcgg gtcgttgagc gtgatcgcgc cgccttcgcc gctggtcacg gtcttgaccg
      481 gatggaagga gaagcaggtc atgtcggaga tggcgcacga cccgaccgga ccttgcgcgc
      541 cgacggagcc gagcgcgtgg caggcgtcct cgatcacgaa ggcgccgtgc gtcccggcga
      601 cctcggcgat gtggccgagg tcgagcgcgc gaccggcgta atgcaccggg atcacgccgc
      661 gaaagcgatg atcgggatgg tcgcgcgcga tcaggttcag cgcatcgttg aagctttcgt
      721 ccgtcatcag gccggtcagc ggatcgacgt cggcgaacac aacccgcgcg ccgcaatagg
      781 cgacgcagtt ggcgctggcc gcgaaggtga cggccgggac gaccgccacg tcctgcgggc
      841 cgaggtcttc gctcaggacg gccagatgca gcgccgccgt gccgttcgcg accacgaccg
      901 cctcctgcgc gccgacggct tgcgccaacg cctgctcgaa ccgctcgacc agcggcccgg
      961 tggtcaggaa atcggatttg agcgccgcca cgaccgccgc gatgtcggcg tcgtcgatcg
     1021 actggcggcc atagggcagg aagctcacga caggtaggcg tccatcatcg cccggatgcc
     1081 ctcggcgtcg aggcgctcgc gattgttgtc ggaggcgtag gaaaagcctt cttcgaccgt
     1141 gcgggcgccg gcggcggcgc ggaagctttt gcgctggtat tcgacgaatt ccggctcgat
     1201 cacatagcgg tcgtccagtt cgacggtcgc gcgcgcgtcg tcggcggaga tcatcatctc
     1261 gtgcagcttt tcgccggggc ggacgccgac gatccggtgc gccgcgccgg gcgccagcgc
     1321 ccgcgccaga tcggtgacca gggtggccgg aatcttcggc acgaagatct cgccgccctg
     1381 catgaggtcg aggctggaca gcacgaaggc gacgccctga tcgagcgaga tccagaagcg
     1441 cgtcatgcgc gggtcggtga tcggcagttc gcgcgcgccg ttctccagca ggcgcttgaa
     1501 atagggcgcg accgaaccgc gcgagccgac gacattgccg tagcgcacga cggagaagcg
     1561 cgtgccgatg tccccggcca gattgttggc ggcgacgaag atcttgtccg aggccagctt
     1621 ggtggcgccg tagagattga ccggcgaaca cgccttgtcg gtcgagagcg cgatcacgcg
     1681 cgccacgcgg ttgtgcaggc aggcccagac gacgttttcg gcgccggt
//
LOCUS       DASVLL010000200         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_226154,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000200 DASVLL010000000
VERSION     DASVLL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226154"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VF402_03270"
     CDS             <1..285
                     /locus_tag="VF402_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX7799342.1"
                     /translation="RGLPTMAQTGYPVMFDATHSVQQPGGQGTTSGGQREFAPVLARA
                     ACAVGVSAVFIETHEDPDHAPSDGPNMIPIGKMRELIGVLRAFDDLAKGR"
     gene            complement(416..1429)
                     /locus_tag="VF402_03275"
     CDS             complement(416..1429)
                     /locus_tag="VF402_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX7799343.1"
                     /translation="MILRARLDKLIGYFRPFDWRHMLVAAPGMAACLIYGLVTGDTLT
                     AAIAAGSAFSVGFGLRRYRSVRSMLGCVALMTAAALIGSLTAGNFALYMVLMAGAAAA
                     CACFALVDDDLWWVALQATIALMLASHYAGDFHAAFARASVVMAAGLFQMACVMALRR
                     IPLGHPETVLNIPIPATKRALLAYGGVAALSVVGAMLIAYGLHMDKAYWAPMTALIIL
                     KPKYHLTRQRGFERLAGNVLGCAIATGITFVIPQNILMGANLDVVLCVLGSGASYALI
                     KARYAAFSLAVSFTVVMLLYSAHASALAGSEQRIYATVIGGLTSIAVMWLASRTIARD
                     YAM"
     gene            1555..>2746
                     /locus_tag="VF402_03280"
     CDS             1555..>2746
                     /locus_tag="VF402_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX7799344.1"
                     /translation="MTNINRRQLGQGFAAALAAAGLAPAAAKAAPMDLSFPKTFRWGV
                     ATASYQIEGAVHEDGRGQTNWDVFSHTPGKTANGDTGDVACDSYHLYPEDVKLMQNLG
                     VGTYRMSLAWSRIFPEGRGQPNQKGIDYYNKVVDTLLAAGIDPYITLFHWDLPQALPG
                     GWQNRDTALAFADYAGFMADKLSDRVHNFMTVNELRCFTDLGHMVGIHAPGLKLDPAG
                     VNQVRHHGVLAHGLGVQAIRAHARAGTQVGLADNTTFYCPVIETPEHIAAAKKATREM
                     NAMFLTAIMEGRYIDEYLHEAGAAAPKVRDGDMAAIASPLDFVALNIYTPEWVRADDG
                     PKGYVVIPHNAESPRMASPWLVVGPEVAYWGPRMVSELWGPKTLYISENGASADDAIV
                     NGHID"
BASE COUNT          545 a          944 c          868 g          389 t
ORIGIN      
        1 cgcggcctac cgacgatggc gcagaccggc tatccggtga tgttcgacgc cacccattcg
       61 gtgcagcagc cgggcgggca ggggacgacc tccggcggcc agcgcgagtt cgcgccggtg
      121 ctggcgcggg cggcctgcgc ggtcggcgtg tcggcggtgt tcatcgagac gcacgaagac
      181 ccggaccacg cgccgtcgga cggcccgaac atgatcccga tcggcaagat gcgggagctg
      241 atcggcgtgc tgcgcgcctt cgacgatctg gcgaagggcc gctgacactt acggggtctg
      301 gggccaattg gccccagtcc tttccttcca acaaaaagcc ctcgccaaag gcgggggctt
      361 tttgttggaa aagaagactt gggggcaatc gcccccaaac cccgtaagtg tcgcgtcaca
      421 tcgcatagtc gcgcgcgatc gtccggctgg ccagccacat caccgcgatc gacgtcagcc
      481 cgccgatcac cgtggcgtag atccgttgct ccgacccggc cagggccgag gcgtgcgccg
      541 aatagagcag catcaccacg gtaaagctga ccgccagcga gaaggccgca taacgcgcct
      601 tgatcaacgc atacgacgcg ccgctgccca gcacgcacaa caccacgtcg agattggcgc
      661 ccatcaggat gttttgcggt ataacaaagg ttatacccgt cgcgatcgcg cagcccagca
      721 cattgcccgc cagccgctcg aaccccctct gtcgcgtcag gtgatatttc ggcttcagga
      781 tgatcagcgc cgtcatcggc gcccaatacg ccttgtccat atgcaggccg taggcgatca
      841 gcatggcgcc gaccacactc agcgccgcca cgccgccata ggccagcagg gcgcgcttcg
      901 tcgccgggat cgggatgttc agcacggttt ccgggtggcc cagcggaatc cgccgcaacg
      961 ccatcacaca cgccatctgg aacaggcccg ccgccatcac cacgctggcg cgcgcgaagg
     1021 cggcgtggaa atcgcccgca taatggctgg ccagcatcag cgcgatcgtc gcctgcaagg
     1081 ccacccacca caggtcgtcg tcgaccagcg cgaaacaggc gcaggccgcc gccgcgcccg
     1141 ccatcaggac catgtagagc gcgaaattcc cggcggtcag cgacccgatc agcgccgccg
     1201 ccgtcatcag cgccacgcag cccagcatcg agcgcacgct gcggtaacgc ctgaggccga
     1261 agccgaccga aaaggcgctg cccgccgcga tcgccgccgt cagcgtgtcg ccggtgacga
     1321 ggccatagat caggcacgcc gccatgcccg gcgccgccac cagcatgtgc cgccagtcga
     1381 agggacggaa ataaccgatc agcttgtcga ggcgcgcgcg caggatcata cccgtcagtt
     1441 aagcgcgcaa cggtaaaaag acgataacaa atcgagttgc ggcgggcgct gaaaagttgg
     1501 atgctcgccc gaccacacca agccgatcat aaacaacaaa atagagagga caccatgaca
     1561 aacatcaaca gacgtcaact ggggcagggc tttgcggcgg cgctggcggc ggcgggactg
     1621 gcgcccgcgg cggcgaaggc ggcgccgatg gacctcagct tccccaagac cttccgctgg
     1681 ggcgtcgcca ccgcatccta ccagatcgaa ggcgcggtcc acgaagacgg tcgcggccag
     1741 accaactggg acgtgttcag ccatacgccg ggcaagaccg ccaacggcga caccggcgac
     1801 gtggcctgcg acagctatca cctctatccc gaagacgtga agctgatgca gaatctcggc
     1861 gtcggaacct atcgcatgtc gctggcctgg tcgcgcatct tccccgaagg acgcggccag
     1921 ccgaaccaga agggcatcga ctactacaac aaggtggtcg atacgctgct ggcggcgggg
     1981 atcgatccct atatcaccct gttccactgg gatctgccgc aggccctgcc gggcggctgg
     2041 cagaaccgcg acacggcgct ggccttcgcc gactatgcgg gcttcatggc ggacaagctg
     2101 tcggaccgcg tgcataactt catgacggtc aacgaactgc gctgcttcac cgatctgggc
     2161 catatggtcg gcatccacgc gccgggcctg aagctggacc cggcgggcgt caatcaggtc
     2221 cgccaccacg gcgtgctggc gcacggtctg ggcgtgcagg cgatccgcgc gcacgccagg
     2281 gcgggaaccc aggtcgggct ggccgacaac accaccttct attgcccggt catcgaaacg
     2341 cccgaacaca tcgccgccgc gaaaaaggcg acgcgcgaga tgaacgccat gttcctgacc
     2401 gcgatcatgg aaggccgcta tatcgacgaa tatctgcacg aagccggcgc cgccgcgccg
     2461 aaggtccggg acggcgacat ggccgccatc gccagcccgc tcgatttcgt cgcgctcaac
     2521 atctatacgc cggaatgggt gcgcgccgac gacggcccca agggctatgt ggtcattccg
     2581 cacaacgccg aatcgccgcg catggcctcg ccgtggctgg tggtcgggcc ggaggtcgcc
     2641 tattgggggc cgcgcatggt cagcgagctg tgggggccga agacgctcta catctccgaa
     2701 aacggcgcct cggcggacga cgccatcgtc aacggccata tcgacg
//
LOCUS       DASVLL010000201         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_226259,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000201 DASVLL010000000
VERSION     DASVLL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226259"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..592)
                     /locus_tag="VF402_03285"
     CDS             complement(179..592)
                     /locus_tag="VF402_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX7799345.1"
                     /translation="MNPKIDWAELFFSSSGRTGQGPSTLAAAVLLLILAMYEALMAGP
                     LQYLTGWIVYPVLLFCGACVLSKRLHDRGRSGWWAAVILAAVIMVWPAPKGFFDFVAM
                     VVLIWAAVDLCVMPGERGDNRYGPSLYKPVTPREA"
     gene            complement(663..1487)
                     /locus_tag="VF402_03290"
     CDS             complement(663..1487)
                     /locus_tag="VF402_03290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7799346.1"
                     /translation="MSKHSPLHLKALARRGPRPQQQPKSVPASKLPRAISAEDRAFVT
                     GMIVYQDELIMGFNKPSNLSSQGGRGPDSHNLDDLMWAFVKPSGRRPMLVHRLDRDTS
                     GIILAARTHPATSYLGKAMIRRAFSKTYLAVVSNPHNLADQVTVEAPLRREEIGREAW
                     SRVCAADHADAQTAVTHIEVLSRGEEAALVRCRPVTGRMHQIRVHLAHLGSPIAGDAR
                     YGGALALGGVAVPRLMLHALSLTFPHPGHGADMTLRAPVPDDMRAVCAALELTLPV"
     gene            complement(1519..>1964)
                     /locus_tag="VF402_03295"
     CDS             complement(1519..>1964)
                     /locus_tag="VF402_03295"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX7799347.1"
                     /translation="FTLLLAWNVIGNLIGMVLVLIVKPKGRNRFGKPGAPCNPIQSIT
                     ACMTQYSSAAGRSSRSEFWWFFLAALVISLLLDGLDSALKVRIFHYGGLGLFLPLICA
                     QVRRLHDINRSGWWVLLNVTLLPIVLWVLYAWPPTKDDEQIANVF"
BASE COUNT          406 a          689 c          566 g          303 t
ORIGIN      
        1 tttgaacacg gacgctggcc tccggccatc cccacggacc gccgctgcac ggcgcgcacg
       61 gaaaacacgg ataagagcag gacgggcaac ggcagcgcca aatctcccgt ccgtgttttc
      121 cgtgccgctg gccgaaggcc agcgcttccg tggtttccgt ggttgaaata cttgaaacct
      181 acgcctcgcg cggcgtcacc ggcttgtaca ggctcggccc gtaacggttg tcgccgcgct
      241 cgcccggcat cacgcacagg tcgacggcgg cccagatcaa caccaccatc gcgacgaaat
      301 cgaaaaagcc cttcggcgcc ggccacacca tgatcacggc ggccaagatc accgccgccc
      361 accagcccga acggccccgg tcgtgcagcc gcttggacaa tacgcacgcg ccgcagaaca
      421 gcagcaccgg atagacgatc cagccggtca gatattgcag cggccccgcc atcagcgctt
      481 cgtacatggc cagaatcagc agcagaaccg ccgccgccag ggtcgacggc ccctgccccg
      541 tccgcccgga tgaggaaaag aacagttcgg cccagtctat cttcggattc atcgccgcgg
      601 cgtccttcac atgcttcgtc tgcccttata gcataaggac aacggagaaa agccgttttc
      661 cttcagaccg gaagcgtcag ctccagcgcc gcgcacacgg cgcgcatgtc gtccgggacc
      721 ggcgcgcgca acgtcatgtc ggcgccgtgt ccgggatgcg gaaaggtcag gctcagcgcg
      781 tgcagcatca ggcgcggaac cgcgacgccg cccagcgcca gcgcgccgcc gtaacgcgca
      841 tcgcccgcta tgggactgcc cagatgcgcc agatgcacgc gaatctggtg catccgcccg
      901 gtgaccgggc ggcagcgcac cagcgccgcc tcctcgcccc ggctcagcac ctcgatatgc
      961 gtcacggcgg tctgcgcgtc cgcatggtcg gcggcgcaga cccgcgacca cgcctcgcgc
     1021 ccgatctcct cgcgccgcag cggcgcctcc accgtcacct gatcggcaag gttgtgcgga
     1081 ttggagacga cggccagata ggtcttggaa aaggcccgcc ggatcatcgc cttgcccaga
     1141 tacgaggtcg ccggatgcgt cctcgccgcc agaatgatcc ccgacgtgtc gcggtccagc
     1201 cggtgaacca gcatcggcct gcgcccgctc ggcttgacga aggcccacat caggtcgtcg
     1261 agattatggc tgtccggccc gcgcccgccc tgcgacgaca ggttggacgg cttgttgaag
     1321 cccatgatca gctcgtcctg atagacgatc atgccggtga cgaaggcccg gtcctccgcc
     1381 gaaatcgcgc gcggcagctt cgacgccgga accgacttcg gctgctgctg aggccgcggc
     1441 ccgcgccgcg ccagcgcctt cagatgtagc ggggaatgtt tggacatacg ccgcctttag
     1501 cgcgagagcg cacaggcgtc aaaagacatt agcgatctgc tcgtcgtcct tcgttggcgg
     1561 ccacgcgtag agcacccata aaacgatcgg caacagggtc acattcagca atacccacca
     1621 gccgcttcga ttgatgtcat gcagacgccg cacctgcgcg caaatcagcg gcagaaacag
     1681 gcccaaccca ccataatgaa aaatccttac cttcaaagcc gagtcaagac cgtccagcag
     1741 cagactgatc acgagagccg cgagaaagaa ccaccaaaac tccgaacgac tgcttcgccc
     1801 cgccgcactg ctgtattgcg tcatgcaggc cgttattgac tgtatcggat tacaaggcgc
     1861 tcccggttta ccgaagcgat tgcggccttt aggcttaacg atcaacacca gaaccatgcc
     1921 gatgagatta ccgatcacat tccaagctaa cagcaaggta aaca
//
LOCUS       DASVLL010000202         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_119410,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000202 DASVLL010000000
VERSION     DASVLL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119410"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VF402_03300"
     CDS             <1..977
                     /locus_tag="VF402_03300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX7799348.1"
                     /translation="DRMEIIRIPGYTEDEKVEIAKRHVLPSLMKEHGLTAEEFVVPEK
                     AIRDVIRYYTREAGVRSLERELGNLARKTIRDLARETKNGEGVKSIEIDDARLARYAG
                     VRRFHYGATDETDQVGIVTGLAWTEFGGDILTIEAIKMPGKGGMKVTGNLKEVMKESV
                     SAANSYVKALAPKFGILPPVFDKTDVHVHVPEGATPKDGPSAGVAMVTAMVSVLTGIP
                     VRKDIAMTGEITLRGRVLPIGGLKEKLLAALRSGIKTVLIPKENEKDLVDLPANVKSG
                     LEIIPVAQVEEVLQHALTKPLTPVDWIEPVIAASASGGGDDADSATAH"
     gene            1330..1602
                     /locus_tag="VF402_03305"
     CDS             1330..1602
                     /locus_tag="VF402_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7799349.1"
                     /translation="MTKAELVAAIADQAGLTKDQAKKALDAFTDSVVAALKKGDDVRL
                     VGFGTFLAVKREAGTARNPRTGETVQRPASVTARFRVGEGLKGALN"
     gene            1739..1814
                     /locus_tag="VF402_03310"
     tRNA            1739..1814
                     /locus_tag="VF402_03310"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1772..1774,aa:Val,seq:tac)
     gene            complement(1985..>2984)
                     /locus_tag="VF402_03315"
     CDS             complement(1985..>2984)
                     /locus_tag="VF402_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX7799350.1"
                     /translation="AAGQDSGASTSLISQTTKAGFDQVYNQYFPDYSGESLLTLVQGA
                     QALTQSLGDLTLVPDNTAGQWWFQEHGFHIKRDYGATAGFNANGFSFAGGRERQVYGN
                     QMLGMYMSYTSASPLPTFAIAYTNMSTSDLTLGAYWRINSGNFKGWAHVGAGYTQFDT
                     TRELITPYVSHIAKAKWNGNSTSAGAGVSYQINAGGIGFRPEVSADYYNLSEDKHSET
                     GGSDYFDLNIADRSGHMLTSRAVLNVSYNRSFVRPEIWAGWKQNISADLPDTVANFTG
                     GDPFTLSAGDLKGGGPVAGFRVSVDNRYSYLGLEGEYEKQDAYTDYTVALRARFQF"
BASE COUNT          554 a          916 c          975 g          539 t
ORIGIN      
        1 tggaccgcat ggagatcatc cgcatccccg gctacaccga ggatgaaaag gtcgagatcg
       61 ccaagcgcca cgtcctgccc agcctgatga aggaacacgg cctgacggcc gaggaattcg
      121 tcgtgccgga aaaggcgatc cgcgacgtaa tccgctatta cacccgtgag gcgggcgtgc
      181 ggtcgctgga gcgcgaactg ggcaatctgg cgcgcaagac gatccgcgac ctggccagag
      241 aaacgaagaa tggcgaaggc gtcaagtcga tcgagatcga cgatgcgcgc ctggccagat
      301 atgcgggcgt gcgccgcttc cattacgggg cgacggacga gaccgatcag gtcggcatcg
      361 tcaccggtct ggcctggacc gagttcggcg gcgacatcct gaccatcgag gcgatcaaaa
      421 tgcccggcaa gggcggcatg aaggtgaccg gcaacctgaa agaggtgatg aaggaatccg
      481 tctcggcggc caattcctac gtcaaggcgc tggcgccgaa gttcggcatc ctgccgccgg
      541 tgttcgacaa gaccgacgtt cacgtccacg tccccgaagg cgccacgccc aaggacgggc
      601 cgtcggcggg cgtcgccatg gtgaccgcca tggtgtcggt gctgaccggc attccggtgc
      661 gcaaggacat cgccatgacc ggcgaaatca ccctgcgcgg ccgcgtgctg ccgatcggcg
      721 gcctgaagga aaagctgctg gcggccctgc gctccggcat caagaccgtg ctgatcccca
      781 aggagaacga gaaggatctc gtcgacctgc cggccaacgt caagtcgggg ctggagatca
      841 ttccggtcgc tcaggtcgag gaggtgctgc aacacgcgct gaccaagccg ctgacgccgg
      901 tggactggat cgagccggtg atcgccgcct cggcctccgg cggcggggac gacgccgaca
      961 gcgccacggc gcattaaaat ggcaagagcc cgtcgcgtca gcggcgggct ttttcacagg
     1021 gttttggcgc gaatcagtgc gcggatacct gattcgagtc cttgcgcgcc aaggcgttgc
     1081 cggggatttg ccgatccggc ggcgtcccgt ttggggacaa aaggcgcaaa acccgtcgtc
     1141 aatgcgccga aactcgcgca aaccctttaa aaaacacggt ttgcagcgca gcaaacgcga
     1201 tttctgtttt gacctccctt aatttttgta gtctctatcg tctcgacccg ttgacgactc
     1261 aaggccatgc cggcgcagcg ggagcaagct gaagccaacc gccccaagcc gggtaaggga
     1321 gagagactta tgaccaaagc tgaactcgtg gccgctatcg ccgatcaggc cggtctgacg
     1381 aaggatcaag ccaagaaggc gctcgacgcc ttcaccgaca gcgttgtggc cgctctgaag
     1441 aagggcgacg acgtgcgtct cgtcggcttc ggcaccttcc tggccgtgaa gcgcgaagcc
     1501 ggcacggcgc gcaatccgcg caccggcgag acggttcagc gtcccgccag cgtcactgcc
     1561 cgcttccgcg tcggtgaggg cctgaagggc gcgctgaact aagccgtccg gcgtatccct
     1621 acgcttttga gagacgtttc cgccagggcg atacgccgga agcggaaacg tctcttgact
     1681 tatgagggcg gagttggcaa agccaccctt ccttgtgacc ctgctttcct tgtgacaggg
     1741 gcgattagct cagctggcag agcggctcgt ttacaccgag taggtcggcg gttcgatccc
     1801 gtcatcgccc accaaattcc cccgacattt ttctgaaata acggatgaac ctaccgtgct
     1861 tttcgcgccg atgcgcgaag cgcgtcgctt ccgtactttc catgttttcc cttctatgtc
     1921 gcaagcgcgc gaaaaagccg ccggggtcgt ccccggcggc tttgcaggct gattttagcc
     1981 cggcttagaa ctggaagcgg gcgcgcaggg cgacggtgta gtccgtatag gcgtcctgct
     2041 tttcgtattc gccttccagg ccgagatagc tgtagcgatt gtcgaccgag acgcggaagc
     2101 ccgccaccgg gccgccgccc ttgaggtcgc cggcgctcag ggtgaacgga tcgccgccgg
     2161 tgaaattcgc caccgtatcg ggcaggtcgg cggaaatgtt ctgcttccag cccgcccaga
     2221 tttccggccg cacgaagctg cggttatagc tgacgttcag cacggcgcgc gaggtcagca
     2281 tatggcccga acgatcggcg atgttcaggt cgaaataatc gctgccgccc gtttcgctgt
     2341 gcttgtcctc gctcagattg tagtagtcgg cgctgacttc ggggcggaag ccgatgccgc
     2401 ccgcgttgat ctggtacgag acgcccgcgc ccgcgctggt cgagttgccg ttccacttgg
     2461 ccttggcgat gtgggagaca tagggggtga tcagctcgcg cgtggtgtcg aactgcgtat
     2521 agcccgcgcc gacatgcgcc cagcccttga aattgccgga attgatccgc cagtaggcgc
     2581 cgagcgtcag gtccgacgtg ctcatattgg tgtaggcgat ggcgaaggtc ggcagcggcg
     2641 aggccgaggt gtacgacatg tacatgccca gcatctggtt gccgtaaacc tgacgctcgc
     2701 ggccgccggc gaagctgaag ccgttggcgt tgaagccggc ggttgcgccg tagtcgcgct
     2761 tgatgtggaa gccgtgttcc tggaaccacc actggccggc ggtgttgtcc ggcaccaggg
     2821 tcaggtcgcc caggctctgg gtcagcgcct gcgcgccctg cacgagggtc agcaggcttt
     2881 cgccggaata gtcggggaaa tactggttat agacctgatc gaaaccggcc ttggtcgtct
     2941 ggctgatcag cgaggtggag gcgccgctgt cctgccccgc cgcc
//
LOCUS       DASVLL010000203         4163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_119419,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000203 DASVLL010000000
VERSION     DASVLL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119419"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2754
                     /locus_tag="VF402_03320"
     CDS             <1..2754
                     /locus_tag="VF402_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799351.1"
                     /translation="DAVIALPGILQTQGFGDEITAETQFVAALPRDMRWYLLPQYKPY
                     TVPGNIQVPYLSQPVRHYLGVAWYQRDIEIPADWSGRRTELLLERTRWRTDVYVDDRR
                     IGDDRSLVAPHSFDLGVLRPGTHRLSIRIDNRMLAPPYRPDGHAVSDAEGSTWNGIVG
                     RIELSATSPVWIEDVQAFPNLSDKSAQVRVTIGNLTGKAGKGVLSAGGASVPVAWDAA
                     GGKAVLDVAMPGARPWSEFTPELQRLSVSLRGDGADHAREVVFGMREIRTDGDKILLN
                     GELFHLRATHDGGGFPLTGYPATDVATWKRIIGICKAWGLNGMRFHSWCPPEAAFIAG
                     DELGFYFQAECGMWNSFDADGAMLAVLNDETARLLKAYGNHPSLIMLGASNEPAGHYA
                     EQLPGWDKRWREADPRRLYTDGTGRWAPPPGGAGAPFAADYLVTGSPARGPRGWFGAD
                     YEDSLNAMRRGAAIPCVGHEIGQYCAYPDFAVIEKFKGEQARYDAYPEGVGAGRTPYM
                     HPGNYIIMRDSAEAHGLLERNKALAHASGRFQLACYKEEIEAQLRTPSYSGYQLLDLH
                     DYLGQGGALIGVLDAFWEEKGYVDAQEFRRFNNATVPLIRLGTRIFTTGQTLSARAET
                     AHFGPEALKAVTPTWRIVDRDGKTMMAGALPVRDIARGKGQALGTIEASLAGLPAPAV
                     YTLVLDLGDAGFSNQWSFWLYPAATDANIPPDVLVTASWEAARARLSGGGKVLFLSGA
                     PENPTRDLALTTTPIFWNRLMNPSRTWMLGLLNASGHGALGGFPTEDNCDWQWVDLLP
                     KTTAMNMEALPPDLTPVVQPIDDWNRNLRLSMLFECQAGKGRLMVTSFDLSDAGVAAK
                     PAAAALRRSVLDYMASPRFAPQHRLTLAALDAWAPTRYVAPVIPTSPPAASDVADPGQ
                     VRQ"
     gene            complement(2765..>4163)
                     /locus_tag="VF402_03325"
     CDS             complement(2765..>4163)
                     /locus_tag="VF402_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX7799352.1"
                     /translation="KNLTGHKILGLAFAGLLYLICLSGAATVFYQTLQLWETAGAPDA
                     AFSAQGVAAAIADGRLRQPKGAVSLSASLPTPDLPRLTVMAGETHLAYDGAGRFAGKT
                     GAPATDGLCALHYELNIPGVAGLILVGIGGVAIVALLIGGLLAHPRIFKDAFVWRFRA
                     GARLNRSDLHNRVGVWASPFHLAIGLTGALIGLSQVILLVAGLTFYKGDLTAAAAPLY
                     GDMSAKTVAGRMDATALTAAFDALRRDFPGARPSYLTLNHLNTDHESLTVWADMPDRL
                     VYAEQFEFDGKGRLSARHHLDDGALGKQVYSSLYRVHFGAFGGVWILWAWCLLGFGLT
                     AICATGMDIWLLKSAQKGKSHPRLHRLWVGFVWAAPAAMCAAALASLIAGAPFAAVFW
                     PALALISVAAVALPNAALAAIVGRATLAALLLMLIGAHIARFGAAGGAGAALWVNLSL
                     LAIALICLLSTLKKA"
BASE COUNT          668 a         1409 c         1477 g          609 t
ORIGIN      
        1 gacgccgtca tcgccctgcc cggcattttg cagacgcagg gattcggcga cgagatcacc
       61 gccgaaacgc agttcgtcgc ggcgctgccg cgcgacatgc gctggtatct tctgccgcag
      121 tacaagccct acaccgttcc cggcaatata caggtgccct acctgtcgca gcccgtgcgc
      181 cactatctcg gcgtggcctg gtaccagcgc gacatcgaga ttccggcgga ctggtctggc
      241 cggcggacgg agctgctgct ggagcggacg cgctggcgca ccgacgtcta tgtcgacgac
      301 cggcggatcg gcgacgaccg ctcgctggtg gcgccgcaca gtttcgatct gggcgtcctc
      361 aggcccggaa cgcaccgcct cagcatccgg atcgacaacc gcatgctggc gccgccctat
      421 cgtcccgacg gccatgcggt ttcggacgcc gaaggctcga cctggaacgg gatcgtgggc
      481 cggatcgagc tgtcggcgac gtcgcccgtc tggatcgagg atgtgcaggc gttcccgaac
      541 ctgagcgata aatcggctca ggtgcgcgtg acgatcggca acctgaccgg caaggcgggc
      601 aagggcgtcc tgtccgcggg cggcgcttcc gttcccgtcg cgtgggacgc ggcgggcggc
      661 aaggccgtcc tcgacgtcgc catgcccggc gcccggccgt ggtcggaatt cacccccgaa
      721 ctccagcgcc tgtccgtgag cctgcgcggg gatggggccg accatgcgcg cgaggtcgtc
      781 ttcggcatgc gcgagatcag gacggacggc gataaaatcc tgctgaacgg cgagctgttc
      841 cacctgcgcg ccacccacga cggcggcggc ttcccgctga cgggctatcc ggccacggac
      901 gtcgcgacgt ggaaacggat catcggcatc tgcaaggcgt ggggcctgaa cgggatgcgc
      961 ttccattcgt ggtgtccgcc cgaggcggcc tttatcgcgg gcgacgagct gggcttctac
     1021 tttcaggccg aatgcggcat gtggaacagc ttcgacgccg acggcgcgat gctggccgtg
     1081 ctgaacgacg agacggcgcg cctgctcaag gcctacggca accatccgtc cctgatcatg
     1141 ctgggcgcca gcaacgagcc cgccggtcat tatgcggaac aactccccgg ctgggacaag
     1201 cgctggcgcg aagccgatcc gcgccgcctc tacaccgacg ggacgggccg ctgggcgccg
     1261 ccgccgggcg gcgcgggcgc gccgttcgcg gcggattacc tcgtcaccgg ctcaccggcg
     1321 cgcggcccga ggggctggtt cggcgcggac tacgaagaca gcctgaacgc gatgcggcgg
     1381 ggggcggcca ttccgtgcgt cggccatgaa atcggtcaat attgcgccta tccggatttc
     1441 gccgtcatcg aaaagttcaa gggcgaacag gcgcgctacg acgcctatcc cgaaggcgtc
     1501 ggcgcgggcc ggacgcccta catgcacccc ggcaattaca tcatcatgcg cgattccgcc
     1561 gaggcgcacg gccttctgga gcgcaacaag gcgctggccc acgcgtcggg gcgctttcag
     1621 ctcgcctgct acaaggagga gatcgaggcc cagctgcgca cgccgtccta ttcggggtat
     1681 caactgctgg atctgcacga ctatctgggg cagggcggcg cgctcatcgg cgtgctggac
     1741 gccttctggg aggaaaaggg ctatgtcgac gctcaggaat tccgccgctt caacaacgcg
     1801 accgtgccgc tgatccggct ggggacgcgg attttcacga ccggccagac cctgagcgcg
     1861 cgcgcggaaa cggcgcattt cggccccgag gcgctgaaag cggtgacgcc gacatggcgg
     1921 atcgtcgacc gcgacggcaa gacgatgatg gcgggcgcct tgcccgtccg cgacatcgcg
     1981 cgcggcaagg ggcaggcgct ggggaccatc gaagcctcgc tcgccggtct gcccgcgccc
     2041 gccgtttata cgctggtgct ggacctcggc gacgccgggt tcagcaatca gtggtctttc
     2101 tggctgtatc cggccgcgac cgacgcgaac attcccccgg acgtgctggt gacggcgtca
     2161 tgggaagcgg ccagggcgcg gctgagcggc ggcggtaagg ttctgttcct gtcgggcgcg
     2221 ccggaaaacc cgacgcgcga tctggccctg accaccacgc cgatcttctg gaaccggctg
     2281 atgaatccga gccggacatg gatgctgggg ctgctgaacg cctccggtca cggcgcgctc
     2341 ggcggatttc cgaccgagga caattgcgac tggcagtggg tcgatctgct gcccaagacg
     2401 acggcgatga atatggaggc cctgccgccc gacctgaccc cggtggtcca gccgatcgac
     2461 gactggaacc gcaatctgcg gctttcgatg ctgttcgagt gtcaggccgg gaagggcagg
     2521 ctgatggtca cgtcgttcga tctgtcggac gcaggcgtcg cggcgaaacc ggcggcggcg
     2581 gcgctgcgcc ggtcggtcct cgactacatg gcctcgccgc gcttcgcgcc gcagcaccgg
     2641 ctcaccctcg ccgcgctcga cgcctgggcg cccacgcgct acgtcgcgcc tgtcatcccg
     2701 acctccccgc ccgccgccag cgacgtcgcc gatcccggcc aggtgcggca atagggcggc
     2761 aggctcacgc ctttttcagc gtcgacagga ggcagatcag ggcgatcgcg agcaggctca
     2821 gattgaccca cagggccgcc ccggcgccgc cggcggcgcc gaatctggcg atatgcgcgc
     2881 cgatcagcat caggagcagg gccgcgaggg ttgcgcgtcc cacgatcgcg gccagggcgg
     2941 cgttcggcag ggcgacggcg gcgacgctga tcagggcgag cgccggccag aagacggcgg
     3001 cgaagggcgc tccggcgatc aggctggcca gcgccgccgc gcacatggcg gcgggcgcgg
     3061 cccagacgaa gccgacccac aggcgatgca ggcgcggatg gctcttgccc ttctgcgccg
     3121 atttcagcag ccatatgtcc atgccggtcg cgcagatcgc ggtcaggccg aagcccagca
     3181 ggcaccacgc ccacaggatc cacacgccgc cgaacgcgcc gaaatgcacg cggtagaggc
     3241 tggaatagac ctgtttgccg agcgcgccgt catcgaggtg atggcgcgcg ctcaggcggc
     3301 ccttgccgtc gaactcgaac tgctcggcat agaccagccg gtccggcatg tcggcccaga
     3361 cggtcagcga ttcatggtcg gtgttcagat ggttgagcgt cagatagctg ggccgggcgc
     3421 cgggaaagtc gcgccgcaag gcgtcgaagg cggcggtcag cgccgtcgcg tccatgcggc
     3481 ccgcgacggt tttggcgctc atgtcgccgt acagcggcgc ggcggcggcg gtcaggtcgc
     3541 ccttgtagaa ggtcaggccc gccaccagca ggatgacctg cgacaggccg atcagcgcgc
     3601 cggtcaggcc gatggcgaga tggaacggcg aggcccagac cccgacgcgg ttgtgcaggt
     3661 cggagcggtt gagccgcgcg cccgcgcgga agcgccacac gaatgcgtcc ttgaagatgc
     3721 gcggatgggc cagcaggccg ccgatcagca gcgccacgat ggccacgccg ccgatcccga
     3781 ccaggatcag gcccgcgacg ccgggaatgt tcagttcgta atgcagggcg cacagcccgt
     3841 ccgtcgcggg cgcgccggtc ttgcccgcga agcggcccgc gccgtcatag gccagatgcg
     3901 tttcgcccgc catgacggtc aggcgcggca ggtccggggt gggcagggag gcggacaggc
     3961 tgacggcgcc tttcggctgg cgcaggcggc cgtccgcgat ggcggcggcg acgccctgcg
     4021 cggaaaaggc cgcgtcgggc gcgcccgccg tctcccagag ttgcagggtc tggtaaaaga
     4081 cggtggccgc gccgctcagg cagatcaggt acagaagccc ggcgaaggcc agaccgagaa
     4141 tcttgtggcc agtcaggttc ttc
//
LOCUS       DASVLL010000204         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_496371,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000204 DASVLL010000000
VERSION     DASVLL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496371"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VF402_03330"
     CDS             complement(<1..210)
                     /locus_tag="VF402_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX7799353.1"
                     /translation="MAAGGDDDNAPRKTVLGFLKSIGPGLITGASDDDPSGVATYSQA
                     GAQFGLLTLWTALITFPLMAAVQEMC"
     gene            complement(214..768)
                     /locus_tag="VF402_03335"
     CDS             complement(214..768)
                     /locus_tag="VF402_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX7799354.1"
                     /translation="MTAAPKRPGSGEQRRRLEEMLRVDHAGELAAVHIYRGQSRVFSD
                     TPHTELSRQFTKMQGEEQVHLDAFETLLRERGVRPTLMTPVWRVAATALGVGTALLGE
                     KAAHACTEAVEAVIGEHYAGQVAELEASDPELAARLAQFRDDEMGHHDQAVAQGAHEA
                     PAYPLLSAVVQAGCRLAIRISEKV"
     gene            complement(765..1319)
                     /locus_tag="VF402_03340"
     CDS             complement(765..1319)
                     /locus_tag="VF402_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX7799355.1"
                     /translation="MQSARSNKTGKKLFLAFGRWWSITALVLSLAMLATAWWFQLFKH
                     LDPCHLCLKQRDIYWIASGVALVASVWALFTGAKGPPRVFSFVLFAIFATGATLALFH
                     AGVELKWWRGPESCTASGGEISLDQMAAFLAGAATRAPMCDVAQWRMFGVSMAGYNAM
                     ISTVLALLSLIASLRFRPRRRRAA"
     gene            complement(1327..1908)
                     /locus_tag="VF402_03345"
     CDS             complement(1327..1908)
                     /locus_tag="VF402_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX7799356.1"
                     /translation="MLRPLYNWMIRQAGGPHAEKALAVIAFAEASFFPLPPDVMLAPM
                     VMARPEKAWRLALICSVFSVIGGFLGYAIGYYVQPLGHWILALFGHPDGLAEFRAFFA
                     KWGVAVILVKGLTPIPYKLVTIAAGLAQFSLLWFALSSAVTRTVRFFFVAFLFRKFGP
                     ELAELIEKRIYVVTAVVLAVIVLGFVIVKWMAK"
     gene            2194..2616
                     /locus_tag="VF402_03350"
     CDS             2194..2616
                     /locus_tag="VF402_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7799357.1"
                     /translation="MPYRLAVASTAVSALIATAYESLYGLKIPEWRLIFVLREDGPTT
                     QQALVKRSGMDKVTISRAAQSLAKRRLITRAPHEHDGRSHHLILTAEGERLYADVAPA
                     AAEYERIMLAEFTPDEVVQFKKLLRRVEEAAVKAHNGR"
     gene            2702..>3356
                     /locus_tag="VF402_03355"
     CDS             2702..>3356
                     /locus_tag="VF402_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799358.1"
                     /translation="MQDGFDAPSAALSDFPSDPVVHHHVLAWLSVAALLVVATATLTL
                     VYKSAERMARNRLGDGVDARVKAVRKTLEAAARARAGDQVELAQAARAALREHFGGVL
                     ELSAHLGKATDGLNKALEGMREEDAKPSSGGAVHGGTIINIAVANGAAAPLPGFGPGP
                     GPEPGPAEPAPESHAESPPESQTDALWRAVQKLFAYWSHMNVVAAAFRAAQTQLLQSP
                     "
BASE COUNT          609 a         1107 c         1139 g          501 t
ORIGIN      
        1 gcacatctcc tgcaccgccg ccatcagcgg aaaggtgatc agcgcggtcc acagggtcag
       61 caggccgaac tgggcgcccg cctgcgaata ggtcgccacg cccgacggat cgtcgtccga
      121 cgcgccggtg atcaggccgg gtccgatgga tttgagaaaa ccgaggacgg tcttgcgcgg
      181 cgcgttgtcg tcgtctcccc ccgccgccat cggtcagacc ttctcgctga tgcggatggc
      241 gaggcggcac ccggcctgca ccaccgccga cagcaacgga taggcgggcg cctcatgcgc
      301 gccctgcgcg acggcctgat cgtgatggcc catctcgtcg tcgcggaatt gcgccagtcg
      361 cgcggccagt tcgggatcgg acgcctcaag ctcggcgacc tgcccggcgt aatgctcgcc
      421 gatcaccgcc tcgaccgcct cggtgcaggc gtgggcggcc ttttcgccga gcagggccgt
      481 gccgacgccc agcgcggtcg ccgccacgcg ccacaccggc gtcatcaggg tcggacggac
      541 gccgcgttcg cgcaacagcg tctcgaaggc gtcgagatgg acctgctcct cgccctgcat
      601 cttcgtgaac tggcggctga gttcggtgtg cggggtgtcg gaaaagaccc ggctctggcc
      661 ccggtagata tgcacggcgg ccagctctcc ggcgtgatcg acgcgcagca tctcctccag
      721 acggcggcgc tgctcgccgg aaccgggacg tttcggcgcg gcggtcatgc ggccctcctg
      781 cggcgcggac ggaagcgcag cgaagcgatc aggctcagta gcgccagcac ggtggaaatc
      841 atggcgttat atccggccat cgacacgccg aacatgcgcc attgcgccac gtcgcacatc
      901 ggcgccctgg tcgcggcgcc ggccaggaag gcggccatct ggtcgaggct gatctcgccg
      961 cccgaagcgg tgcagctttc cggcccgcgc caccatttca gctcgacccc ggcgtggaac
     1021 agggccagcg tcgcgccggt ggcgaagatg gcgaacagca cgaaggagaa gacgcgcggc
     1081 ggccccttgg cgccggtgaa cagggcccag acgctggcga ccagcgccac gcccgaggcg
     1141 atccagtaga tgtcgcgctg cttcaggcac agatggcagg gatcgaggtg tttgaacaac
     1201 tggaaccacc acgccgtcgc cagcatggcc agcgacagca cgagggcggt gatgctccac
     1261 caccggccaa aagcgagaaa caacttttta ccggtcttgt tcgatctggc ggactgcatc
     1321 tcgatcctac ttcgccatcc atttgacgat gacgaagcct agcacgatca ccgccagaac
     1381 aacagccgtg acgacataga tgcgcttctc gatcagctcg gccagctccg gcccgaactt
     1441 gcggaacagg aaggcgacga agaagaagcg caccgtgcgg gtgacggccg acgacagcgc
     1501 gaaccacaga agcgagaact gcgccagacc ggcggcgatg gtcaccagct tgtacgggat
     1561 cggcgtcagg cccttgacga ggatcaccgc cacgccccac ttcgcgaaga aggcgcgaaa
     1621 ctccgcaagg ccgtcgggat ggccgaacag ggccaggatc cagtggccca gcggctggac
     1681 gtaataaccg atcgcatagc cgaggaagcc gccgattacc gaaaagaccg aacagatcag
     1741 ggccagccgc cacgccttct ccggccgcgc catcaccatc ggcgccagca tgacgtcggg
     1801 cggcagcgga aagaaggagg cttcggcgaa ggcgatgacg gccagcgcct tttcggcgtg
     1861 cggtccgccg gcctgacgga tcatccagtt atagaggggg cgcagcacgg gaaactcgga
     1921 aaaaacagcg attccccctc cttaacaggc ggcgcgcggc ttgtcgcgga gaaggcggcg
     1981 ggacggggga aaaattccgc cgccgcgccg aattgacgca gggccggcgc ggctccggcg
     2041 cattagtctt tgtgcagaca gcctttataa ttaatgattt gtcactggac ggatggtttg
     2101 ccattgggtt atgcggccgt atcctttata aacggggccg aggttgagga cacaagatcg
     2161 atgacgcacg gttcgcttac ccttgatgag tacatgcctt atcgcctcgc cgtggcgtcg
     2221 accgcggtga gcgcgctgat cgcgacggcc tatgagagcc tttatggcct gaagataccg
     2281 gaatggcggc tgatcttcgt gctgcgcgag gacggtccga cgacgcagca ggcgctggtc
     2341 aagcgttcgg gcatggacaa ggtgacgatc tcgcgcgcgg cgcagagcct ggccaagcgc
     2401 cgcctgatca cgcgcgcgcc gcatgagcat gacgggcgtt cgcaccacct gatcctgacc
     2461 gccgagggcg agcgcctgta cgccgacgtc gcccccgccg ccgccgagta cgagcgcatc
     2521 atgctggccg aattcacccc ggacgaggtg gtgcagttca agaagctgct gcgccgcgtc
     2581 gaggaagccg ccgtcaaggc gcataacgga cgctgatcat ttaccgcttg cccgctggcc
     2641 tgcggcgggg caatctgtta acctttccgt agcgacgcag atgagcgggg agggggctga
     2701 catgcaggac ggttttgacg cgccttccgc cgcgctcagc gactttccgt ccgatccggt
     2761 cgtgcatcat catgtgctgg cctggctaag cgtggcggcg ctgctggtcg tcgcgacggc
     2821 gaccctgacc cttgtttaca aaagcgccga gcgcatggcg cgcaaccggc tgggcgacgg
     2881 cgtcgacgcg cgcgtcaagg cggtgcgcaa gacgctggag gccgccgcgc gcgcgcgcgc
     2941 gggcgatcag gtcgaactgg cgcaggcggc gcgcgctgcg ctgcgcgagc atttcggcgg
     3001 cgttctggaa ctcagcgcgc atctcggcaa ggcgacggac gggctgaaca aggcgctgga
     3061 aggcatgcgc gaggaggacg cgaagccgtc gtccggcggc gccgtccacg gcggcacgat
     3121 catcaatatc gccgtcgcca acggcgcggc cgcgccgttg cctgggtttg ggcctgggcc
     3181 tgggcctgaa cctgggcctg ccgaaccggc gccggaaagc cacgccgaaa gcccgccgga
     3241 gagccagacc gacgcgctct ggcgcgcggt gcaaaagctg ttcgcctact ggagccatat
     3301 gaacgtggtc gccgccgcct tccgcgccgc gcagacgcaa ttgctgcaat ccccgg
//
LOCUS       DASVLL010000205         4231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_496523,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000205 DASVLL010000000
VERSION     DASVLL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496523"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VF402_03360"
     CDS             <1..363
                     /locus_tag="VF402_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7799359.1"
                     /translation="DGRVMDLSRGAAKALGVYATGTARVRITWLGPADRVAAAPVYTA
                     SAATGDDARYIVQLGAFSQKDNARRAQDRMETAHMDRRNGLYVVYLGPFRGAAAAEGQ
                     RQAAIDAGFHDAVLTHAD"
     gene            418..1086
                     /gene="tmk"
                     /locus_tag="VF402_03365"
     CDS             418..1086
                     /gene="tmk"
                     /locus_tag="VF402_03365"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7799360.1"
                     /translation="MSRIETIEQSRGRFIVFEGGEGAGKSTQLKALVARLRDGGLEVV
                     ATREPGGSPGAEALRDLLVRGEATRWSSLSELLMMYAARSDHLEKVIRPALARGAWVV
                     CDRFADSSRAYQGAGGGVDAELIEQIDAAVVNESQPDLVLVMDMPPEAGLARAQARGD
                     TENRFETKGLAFHARLREGFLRRAAAAPERYRVIDADRSVDAIASDVWARVAETFPEV
                     SRAI"
     gene            1083..2078
                     /locus_tag="VF402_03370"
     CDS             1083..2078
                     /locus_tag="VF402_03370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX7799361.1"
                     /translation="MSEDVGLIAPRANFAFEHGEALDRAFLTAFAKGRLHHAWLLCGP
                     QGLGKASFAYRCARFLMGQRRDPRDLAMSPDDPDVRLITAGSHPDLLVLEREMGDGKL
                     KKSISVDAVREVGEFFSKAPSRSAYRVCIIDSVDDLNINSANALLKILEEPPQNGVLF
                     LISHSPGRLLATIRSRCRRLTFTPWTDEQVAGFVRRRIDLDDDSRTRLVHLAHGAPGR
                     ALSLLEEGALELDAFAERLLSQPLPPRAELTAVATGFKANSAKNNGAQKFATFMACLT
                     DRVHERALSAETPQKGQGLAQLWSRLSQAVGETEAVNLDRADYFWSVYNELARIN"
     gene            2097..2888
                     /locus_tag="VF402_03375"
     CDS             2097..2888
                     /locus_tag="VF402_03375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7799362.1"
                     /translation="MRSRQYIDSHVNLHAPQFDEDREAVIARAREAGVRLMLNICDRV
                     SNFDAVYALAEAHGDIWATVGTHPHEARENPDLTARDLLALAGKPKVVGIGECGLDFH
                     YDYSPRDVQAQVFRAHIAAARDSGLPLVVHTREADDAMGEILEDEFAKGAFKMLLHCY
                     TSGADLARRCAVRGAWFSVSGIASFRAAEEVRAVIRDMPADRIIVETDCPYLAPMPMR
                     GRRNEPAFVPYVYDKLAEIRGWTIEDTITRSDDAFFRLFDRIPRS"
     gene            2885..3688
                     /locus_tag="VF402_03380"
     CDS             2885..3688
                     /locus_tag="VF402_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7799363.1"
                     /translation="MSRHLRVRILGSGCSTGVPRIDGYWGACDPENPRNRRTRCSLWL
                     GLYDGDAATSVVVDTSPEFREQMVRAGVRKLDAVLWTHDHADQTHGIDDMRAYTFARE
                     GVIAGYMDAVTHATLTQRFGYIFEGKFGYPPICAAHILPPHGTPWRIAGEGGELPVLT
                     FDQAHGPIRSVGYRFGPVAYSSDVSDIPEDSFAALQGVKVWIVDALRLRSHPTHAHLD
                     LALQWAERVRPELTILTNLHQDMDYEALQAQLPPGVVPAYDQMELNLTL"
     gene            complement(3692..3973)
                     /locus_tag="VF402_03385"
     CDS             complement(3692..3973)
                     /locus_tag="VF402_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799364.1"
                     /translation="MAISSVHFEPTTSTFGDLNVGTAPPQTAILSANVTPQQRQALVH
                     ILIALGFILAFVVALSALYLGDQLLFSHSHIVTASQPAATYNGFPVDYD"
BASE COUNT          747 a         1277 c         1429 g          778 t
ORIGIN      
        1 gacgggcgcg tgatggatct gtcccgcggc gcggccaagg cgctgggcgt gtacgccacc
       61 ggcacggcgc gggtgcgcat cacctggctc ggtcctgcgg accgggtcgc ggccgcgccg
      121 gtctataccg ccagcgcggc gaccggcgac gatgcgcgct atatcgtgca actgggcgcc
      181 ttttcgcaaa aggacaacgc ccggcgcgcg caggatcgca tggagaccgc gcacatggac
      241 cggcgcaacg gcctctatgt cgtctatctc ggtccgttcc gtggcgccgc cgccgccgaa
      301 gggcaaaggc aggcggcgat cgatgcgggc tttcacgacg ccgtccttac gcacgccgat
      361 taaattcctg tcgattgggt cttgagaatt gcggcgaaac ccggtctatc tgcggctgtg
      421 agcaggatcg aaaccataga gcaaagccgt ggacgtttca tcgtcttcga aggcggcgag
      481 ggcgcgggca agtccacgca gttgaaagcg ctcgtcgcgc gcctgcgtga cggcgggctg
      541 gaggtcgtcg cgacgcgcga acccggcgga tcgcccggcg ccgaggccct gcgcgacctg
      601 ctggtgcgcg gcgaggcgac gcgctggtcg tcgctgagcg aacttttgat gatgtacgcg
      661 gcgcgcagcg accacctcga aaaggtgatc cggcccgccc tggcgcgcgg cgcctgggtc
      721 gtctgcgacc gcttcgccga ttcgtcgcgc gcctatcagg gcgcgggcgg cggcgtcgac
      781 gccgaattga tcgagcagat cgacgccgcg gtcgtcaatg agtcgcagcc cgatctggtg
      841 ctggtcatgg acatgccgcc agaagcgggt ctggcgcggg cgcaggcgcg cggcgatacg
      901 gaaaatcgtt ttgaaaccaa gggattggcc tttcatgccc gtctgcgcga gggctttttg
      961 cgccgcgcgg cggccgcgcc ggagcgatac cgcgtcatcg acgccgaccg atcggtcgac
     1021 gccatcgcca gcgacgtctg ggcgcgggtc gcggaaacgt tcccggaagt gagcagggcg
     1081 atatgagcga ggacgtcggc ctgatcgctc cgcgcgccaa cttcgccttc gagcatggcg
     1141 aggcgctgga ccgcgccttt ctgaccgcct tcgccaaggg gcggttgcat catgcctggc
     1201 tgctgtgcgg gccgcaggga ttgggcaagg ccagtttcgc ctatcgctgc gcgcgcttcc
     1261 tgatgggaca gcggcgcgat ccgcgcgatc tggccatgtc gccggacgat cccgacgtgc
     1321 ggctgatcac cgccggttcg cacccggatc tgctggtgct ggagcgcgaa atgggcgacg
     1381 gcaagctgaa gaagtcgatc agcgtcgacg cggtgcgcga ggtcggcgaa ttcttctcca
     1441 aggcgccgtc gcggtcggct taccgggtct gcatcatcga ctccgtcgat gatttgaata
     1501 tcaattccgc caacgcctta ttgaagattc ttgaggagcc gccgcaaaac ggcgtgctgt
     1561 tcctgatctc gcattcgccg ggccgcctgc tggcgaccat ccgctcgcgt tgccgtcgcc
     1621 tgaccttcac gccgtggacg gacgaacagg tggcgggctt cgtgcgccgg cgcatcgatc
     1681 tcgacgatga cagccggacg cggctcgtgc atctggcgca tggcgcgccg gggcgggcgc
     1741 tgtcgctgct ggaggagggc gcgctggaac tggacgcctt cgccgaacgg ctgctgagcc
     1801 agcccttgcc gccgcgcgcc gagctgacgg cggtcgcgac cggtttcaag gcgaattcgg
     1861 ccaagaacaa cggcgcgcag aagttcgcca ccttcatggc ctgcctgacg gaccgcgtac
     1921 atgaacgcgc cctgagcgcc gagacgccgc aaaaagggca aggtctggcg caactgtggt
     1981 cgcgcctcag tcaggcggtg ggggaaaccg aggcggtcaa tctggacaga gcggattatt
     2041 tctggtctgt ttacaatgaa ttggcgcgta taaattaagg tagatcagga gctttggtgc
     2101 gttcgcgtca atatattgat tctcacgtca acctgcatgc gccgcagttc gatgaggacc
     2161 gcgaggcggt catcgcgcgt gcgcgtgagg cgggcgttcg gctgatgttg aatatctgcg
     2221 accgcgtctc gaatttcgac gcggtttacg cgctggccga ggcgcacggc gatatctggg
     2281 cgacggtggg cacgcatccg catgaggcgc gcgaaaaccc ggacctgacg gcgcgcgacc
     2341 tgctggcgtt ggccgggaag cccaaggtgg tcggcatcgg cgaatgcgga ctcgattttc
     2401 attacgatta cagcccgcgc gacgtgcagg cgcaggtttt ccgcgcccat atcgccgccg
     2461 cgcgcgacag cggcctgccg ctggtcgtgc atacgcgcga agccgacgac gcgatgggcg
     2521 aaattctcga ggacgaattc gccaagggcg cttttaagat gctgctgcac tgctacacca
     2581 gcggcgcgga tctggcgcgg cggtgcgcgg tgcgcggcgc ctggttttcc gtgtcgggca
     2641 tagccagttt ccgggcggcg gaagaggtgc gcgcggtgat ccgcgacatg cccgccgacc
     2701 ggatcatcgt cgagacggat tgtccctatc tggcgcccat gccgatgcgc gggcggcgga
     2761 acgaaccggc cttcgttcct tatgtctatg acaagctggc cgagattcgc ggctggacga
     2821 tcgaggatac gatcacgcgc agcgacgacg cttttttccg tttgttcgac aggattccac
     2881 gctcatgagc cgccatctgc gcgtcaggat tctgggcagc ggctgctcca ccggcgtgcc
     2941 gcgcatcgac ggttattggg gggcgtgcga cccggaaaac cccaggaacc gccgcacgcg
     3001 ctgttcgcta tggctggggc tttatgacgg agacgcggcg acctctgtgg tggtcgacac
     3061 ctcgccggaa ttccgagaac agatggtccg cgccggggtg cgcaagctgg acgcggtgct
     3121 gtggacgcac gatcacgccg accagacgca cggcatcgac gatatgcgcg cctacacctt
     3181 cgcgcgcgaa ggcgtgatcg caggttacat ggacgcggtc acgcacgcca ccttgacgca
     3241 gcgtttcggc tatattttcg aaggaaaatt cggctatccg ccgatctgcg cggcgcatat
     3301 cctgccgccg cacggaacgc cgtggcgcat cgcgggcgag gggggcgaac tgcccgtcct
     3361 gaccttcgat caggcgcacg ggccaatccg ctcggtcggt tatcgtttcg gaccggtggc
     3421 ctattcgagc gacgtttcgg acattccgga agacagcttc gcggccttgc agggggtgaa
     3481 ggtgtggatc gtcgacgcct tgcggctgcg gtcgcatccg acccatgcgc atctcgatct
     3541 ggccctgcaa tgggccgagc gcgtcaggcc ggagctaacc atcctgacca acctgcatca
     3601 ggatatggat tacgaagcct tacaggcgca attgccgccc ggcgtcgttc cggcctatga
     3661 ccagatggaa cttaatctca cgctttaaga tttagtcgta atcgacggga aaaccgttat
     3721 aggtcgccgc cggttgcgag gccgtgacga tatggctatg cgagaaaaga agctgatcgc
     3781 cgagatacag cgcgctgagc gccacgacga aggccaggat gaaaccgagc gcgatcagaa
     3841 tgtgaaccag cgcctgacgc tgctgcggcg tcacattggc gctcaagata gcggtctgcg
     3901 gcggcgcggt tccgacgttc agatcgccga aggtgctggt ggtcggctca aaatgcacgc
     3961 ttgagatggc catgtcggtt tatccttgta cgaaaacaac aaggccaacc taacggcatg
     4021 cggataaaag gtatgtgccg aagccattta agccgtgtaa gcggacgata agccggattc
     4081 aaggcgtttc atatgtcata atttctcata atctttctta ggcgaaaata ctatgtagct
     4141 gggtggattc caatttgaag tgcgacttgt cttggttttc aatgccctac gggcatcaag
     4201 ggacaagcgc tatgggagcc tgttattcgc a
//
LOCUS       DASVLL010000206         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_173917,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000206 DASVLL010000000
VERSION     DASVLL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173917"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VF402_03390"
     CDS             <1..503
                     /locus_tag="VF402_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799365.1"
                     /translation="LKRQAIPAILQSAITKPYDLTGLDTCEAIAGEVAQLDVLLGADF
                     DEPPPPANDDSMTEKGQRMAGHAAVGAVRSASRSIIPFRGIVRKMTGAERHEREVDTA
                     IQAGKVRRAYLKGVGMNKNCAPPAAPSWFKPRVYMQTYTDGQQAQPAAAPPPAPQKRK
                     SNRRYR"
     gene            complement(494..1609)
                     /gene="tgt"
                     /locus_tag="VF402_03395"
     CDS             complement(494..1609)
                     /gene="tgt"
                     /locus_tag="VF402_03395"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7799366.1"
                     /translation="MSSFPFEIHASQGQARTGVLKTPRGDIPTPIFMPVGTAATVKAL
                     TLDMVRQAHAKIILGNTYHLMLRPGPERVARLGGLHKFMGWDGPILTDSGGFQVMSLS
                     NISKVTEDAVTFRSHIDGSKHALSPERSIEIQADLLGSDIVMQLDECVSWPAEEKRAQ
                     AALQLSARWGLRSKQAFGTRDAQALLGIQQGSTFERLRRESTERLLDIGFDGYAIGGL
                     AVGEGHEAMCDTLDYAAPLLPWDRPRYLMGVGKPIDLLEGVARGVDMFDCVLPTRSGR
                     HGQAWTWDGPVNMKNARFAEDDAPLDPDSDCPASRDYSRAYLHHLVRAEEILGQVLLS
                     WHNIAFFQALMIRMRAAILDGTFDALRRDLGARWRQR"
     gene            complement(1606..2664)
                     /gene="queA"
                     /locus_tag="VF402_03400"
     CDS             complement(1606..2664)
                     /gene="queA"
                     /locus_tag="VF402_03400"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7799367.1"
                     /translation="MRISDFDFDLPEDRIALRPMQPREAARLLRVDGGGLTDARVGDI
                     GRFLRPGDLLVVNDTRVLPVQLQGRRVRDDGEVMLEATLIKALDGARWQAFLKPGRRV
                     KPGDRVAFGDNSCELQADVESKGEDGVYTLAFRHAPDIVLERLHTIGAMPLPPYIRSK
                     RREDEQDKSDYQTVFAREEGSVAAPTAGLHFTTDLLDSLSAQGVGVERITLHVGAGTF
                     LPVKVEDTDDHVMHAEFGVISAGSAARINAARAAGGRIVCVGTTSLRLLESACDDRGV
                     IHPFAAETAIFITPGVRVRSCDLLMTNFHLPKSTLFMLVCALAGTETMKRAYAHAIAG
                     GYRFFSYGDACLLTNTSK"
     gene            complement(2696..3106)
                     /locus_tag="VF402_03405"
     CDS             complement(2696..3106)
                     /locus_tag="VF402_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799368.1"
                     /translation="MPLRPRVSLSVDKALNILTLRYIGDMDGATLYAQLLDQFLRVGD
                     PWTYDLIVDFSRYEGVLLTSDNEAFGRVWEDLAQGRDAGRLMAIVSRDPLIIARKAVR
                     AYIFPNFIIDSFITCAEAQDWICDHRGLPRAVCA"
BASE COUNT          556 a         1037 c         1096 g          494 t
ORIGIN      
        1 acctgaaacg gcaggccatt ccggcgatct tgcagagcgc gatcaccaag ccttacgatc
       61 tgaccggact ggatacgtgc gaggcgatcg cgggcgaggt cgcgcagctc gacgtgctgc
      121 tgggcgcgga tttcgacgag ccgccgccgc ccgccaatga cgacagcatg acggaaaaag
      181 ggcagcgcat ggccgggcac gccgccgtcg gcgccgtgcg cagcgccagc cggagcatca
      241 tccccttccg cggcatcgtg cgcaagatga ccggcgccga gcgccacgag cgcgaggtcg
      301 acaccgcgat tcaggcgggc aaggtgcggc gcgcctatct caagggcgtc ggcatgaaca
      361 agaattgcgc cccgcccgcc gcgccgtcat ggttcaagcc gcgcgtctat atgcagacct
      421 ataccgacgg ccagcaggcc cagcccgcgg ccgcgccgcc gcccgcgccg cagaaacgca
      481 agagcaatcg ccgctatcgc tgacgccagc gggcgccgag gtcgcggcgc agggcgtcga
      541 acgtcccgtc gagaatggcc gcgcgcatgc gaatcatcag cgcctgaaag aaggcgatgt
      601 tgtgccacga cagcagcacc tggcccagaa tctcctcggc gcgcaccaga tggtgcagat
      661 aggccctgga ataatcgcgg ctggccggac agtcgctgtc ggggtccagc ggcgcgtcgt
      721 cctcggcgaa gcgggcgttc ttcatattga ccggcccgtc ccaggtccac gcctgaccgt
      781 ggcggcccga gcgcgtcggc aacacgcagt cgaacatgtc gacgccgcgc gccacgcctt
      841 ccagcaggtc gatcggcttg cccaccccca tcaggtagcg cgggcggtcc cacggcagca
      901 gcggcgcggc gtagtccagc gtgtcgcaca tcgcctcatg gccttcgccg accgccagcc
      961 cgccgatggc gtagccgtcg aagccgatgt cgagcaggcg ctccgtcgat tcgcggcgca
     1021 ggcgttcgaa ggtcgagcct tgctggatgc cgagcagggc ctgcgcgtcg cgcgtcccga
     1081 acgcctgttt cgagcgcagc ccccagcgcg ccgacagttg cagcgcggcc tgggcgcgtt
     1141 tttcctcggc gggccatgac acgcattcgt cgagttgcat cacgatgtcc gaaccgagca
     1201 ggtcggcctg aatctcgatg gagcgttccg gcgacagcgc gtgtttcgag ccgtcgatat
     1261 ggctgcggaa ggtgacggcg tcttccgtga ctttggagat gttcgacagg ctcatgacct
     1321 gaaaaccgcc ggaatcggtc aggatcgggc cgtcccagcc catgaactta tgcaggccgc
     1381 ccagccgcgc cacgcgctcc ggtccgggac gcagcatcag gtggtaggtg ttgccgagaa
     1441 tgatcttcgc atgcgcctga cgcaccatgt cgagcgtcag cgccttgacc gtcgctgccg
     1501 tgccgaccgg catgaagatc ggcgtcggaa tgtcgccgcg cggcgttttc agaacgcctg
     1561 tgcgggcctg gccttgcgag gcatggattt caaaaggaaa gctactcatt tcgatgtgtt
     1621 cgtcagcaaa caggcgtcgc cataggagaa gaagcgatag ccgcccgcga tggcgtgggc
     1681 gtaggcgcgc ttcatcgttt ccgttccggc cagcgcgcac accagcatga acagggtcga
     1741 tttcggcagg tggaagttgg tcatcagcag gtcgcaggac cgcacgcgca cgcccggcgt
     1801 gatgaagatg gcggtttcgg cggcgaacgg atggatgacg ccccggtcgt cgcaggccga
     1861 ttccagcagg cgcaagctgg tggtgccgac gcagacgatg cggcccccgg cggcgcgcgc
     1921 ggcgttgatg cgggcggcgc tgccggcgga gatcacgccg aattcggcgt gcatgacgtg
     1981 atcgtccgtg tcctcgacct tgaccggcag gaaggtgccc gcgccgacgt gcagggtgat
     2041 gcgctcgacg cccacgccct gcgccgacag gctgtccaga aggtcggttg tgaaatgcaa
     2101 gcccgccgtc ggggcggcga cggagccttc ctcgcgcgcg aatacggtct ggtaatccga
     2161 cttgtcctgt tcgtcctcgc ggcgtttgga gcggatatag ggcggcagcg gcatggcgcc
     2221 gatcgtatgc agccgttcca gcacgatgtc gggcgcgtgg cggaaggcga gggtgtaaac
     2281 gccgtcctcg cccttgcttt cgacatcggc ttgcaattcg caactgttgt cgccgaacgc
     2341 cacgcggtcg cccggcttca cgcgccgacc gggcttcagg aaggcctgcc atcgggcgcc
     2401 gtccagcgcc ttgatcagcg tggcctccag catgacttcg ccgtcgtcgc gcacgcggcg
     2461 gccctgcaac tgcacgggca ggacgcgcgt atcgttgacc accagcagat cgccggggcg
     2521 caggaagcgg cctatgtcgc cgacgcgcgc gtcggtcagg ccgccgccgt cgacgcgcaa
     2581 gaggcgcgcc gcctcgcgcg gctgcatggg acgcagggcg atgcggtctt cgggcaggtc
     2641 gaaatcgaaa tcggaaatgc gcatcgcgcc gtcaaacgcg caaacgccgc tcaggtcaag
     2701 cgcaaacggc gcgcggcagg ccgcgatggt cgcagatcca gtcctgcgcc tcggcgcaag
     2761 tgatgaagga gtcgatgatg aaattgggga aaatgtaggc gcggacggct ttgcgcgcga
     2821 tgatcagcgg gtcgcgcgac acgatggcca tcaggcggcc cgcgtcgcgg ccctgcgcca
     2881 ggtcttccca gaccctgccg aaagcctcgt tgtcgctggt cagcaggacg ccttcatagc
     2941 gggagaaatc gacgatcagg tcgtaggtcc acggatcgcc gacgcgcaga aactgatcga
     3001 gcagttgcgc gtaaagcgtc gcgccgtcca tgtcgccgat atagcgcagg gtcaggatat
     3061 tgagggcctt gtcgacggac aggctgacgc ggggacgcag gggcatgacg ggccggggtt
     3121 acggaaacac cggcgtatgt taccgccaac cctttaacgc cgcgttagct ggccagaagc
     3181 aag
//
LOCUS       DASVLL010000207         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_442664,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000207 DASVLL010000000
VERSION     DASVLL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442664"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF402_03410"
     CDS             complement(<1..408)
                     /locus_tag="VF402_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799369.1"
                     /translation="MKSTGKAWLLAFAALASAPAAARPQQTDAARVLQVSAQARTPED
                     VFGPLFRAIQLAHVFPDNKTFADMVPRQSPDAILAAYRQQNPTGKEALTAFVARYFVE
                     QNGAPDKNLGLRAHIRSLWPVLARAPLAVTPGSS"
     gene            complement(480..>1571)
                     /locus_tag="VF402_03415"
     CDS             complement(480..>1571)
                     /locus_tag="VF402_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799370.1"
                     /translation="NNILLALEPQARVLNATLDDGTILTRDGFTTGNSYDVNARYTSS
                     NVAGYLSDEWKLGKLRLDGGVRVEHTKLHTVFENSSTGDLDGNPLTAYNNDAAYLNGT
                     YTVAGYHKTQVSWTAGADYAFTPRLNGFVRANGGALLPQFDDLRGNLGSLGDIKPQTV
                     KQYEVGLKTSTPFYDLYLTGFYNRFHNLFFQDIQIVNGQEQNVTAFGGSRAYGLEFDG
                     AVRPVRGFEIATHGVVQNGKFKDFGDNTGNDVNRQPRFMASVTPSYTFDTDWGRARLF
                     GTYTHVGDRYADRENLQKLGSYNTLDLGASVDINQRFSIQATVENVTNTLALTEGNIR
                     TATAGNTNGYFLGRPIFGRHATITAAVKL"
BASE COUNT          279 a          462 c          521 g          309 t
ORIGIN      
        1 ggacgatcct ggcgttaccg ccagcggcgc ccgcgcaagg accggccata gcgagcggat
       61 atgggcgcgc agccccaaat tcttgtccgg ggcgccattc tgttcgacga aatagcgcgc
      121 gacgaaggcg gtgagcgcct ccttgccggt cgggttctgt tgccgatagg cggcaaggat
      181 cgcgtccggc gactgacgcg ggaccatatc ggcaaaggtc ttgttgtccg gaaagacatg
      241 cgcgagttga atcgcgcgaa acagtggccc gaacacgtcc tcgggcgtcc tggcctgtgc
      301 tgaaacctgc agcacgcgcg ccgcgtccgt ctgctgcggc cgcgctgccg caggcgcgct
      361 cgccagtgcg gcgaatgcca gcagccatgc cttgccagtc gatttcatcg tcggaaccct
      421 gcaatcgaaa aaagagtcgg ccgggggcgg ttaggccacc cggccgacag ggggaggtat
      481 cagagtttca ccgcggcggt tatcgtcgcg tgacgcccga agatcggccg gcccaggaaa
      541 tagccgttcg tattgccagc ggtcgccgtg cggatattgc cttcggtgag cgcgagcgtg
      601 ttggtgacgt tctcgacagt cgcctggatc gagaagcgct ggttgatatc aaccgacgct
      661 cccagatcga gcgtgttata gctgccgagc ttctgcaggt tttcacgatc ggcatagcga
      721 tcgccgacat gcgtataggt gccgaacaat cgcgcccgac cccaatcggt gtcgaacgtg
      781 taggacggcg tgaccgacgc catgaatcgc ggctgccgat tcacgtcatt gccggtgttg
      841 tcgccgaaat ccttgaactt gccgttctgg accactccgt gcgtcgcgat ttcgaacccg
      901 cgcaccggcc gcacggcgcc atcgaactca agcccatagg cgcgagagcc accgaaggcg
      961 gtgacgttct gttcctgccc gttgacgatc tggatgtcct ggaagaacag attgtggaag
     1021 cggttgtaga agcccgtcag atagagatcg tagaatgggg tcgatgtctt caggccgact
     1081 tcatattgct tcacggtctg cggcttgatg tcgccgagcg agccgaggtt gccgcggaga
     1141 tcgtcgaact gaggcaacaa tgcgccgccg tttgcacgaa cgaagccgtt caggcggggc
     1201 gtaaaggcgt aatcggcgcc cgccgtccag gatacctgcg tcttgtggta gccggcgacg
     1261 gtgtaggtgc cgttcagata ggcggcgtcg ttgttatagg ccgtcagcgg attgccatcg
     1321 agatcccccg tcgagctgtt ctcgaacacg gtgtgcagct tggtgtgttc cacgcgcacg
     1381 ccgccgtcga ggcgcagctt gcccagcttc cattcgtcgg acaggtagcc cgcgacgttg
     1441 ctgctcgtat agcgggcatt gacgtcgtag ctgttgcctg tggtgaagcc gtcacgagtg
     1501 aggatcgtgc cgtcgtccag cgtcgcgttg agcacgcgcg cttgcggctc gagagcgagc
     1561 aggatgttgt t
//
LOCUS       DASVLL010000208         4564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_442716,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000208 DASVLL010000000
VERSION     DASVLL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442716"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VF402_03420"
     CDS             complement(<1..1356)
                     /locus_tag="VF402_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7799371.1"
                     /translation="MAPSRILILGGGAAGWLSAAYLARSLPDAAITLVESPAIATVGV
                     GEGSFPSLRNTLSFIGLDERAFLRDADAAFKQGVVFDGWKAAGTSYFHPFNAPHRAGP
                     VDLAPYWLLRRAAGAATPFADAVTLQGEVVRQGRAPKRLSDPDFAGPMNYAYHFDAAK
                     LAEALAARARTLGVRHLQGEADGVTLAPDGRIAGVHVAGHGDLSADLYLDCTGFRARL
                     IGEALGARFRPAGDSLFNDRAVALQLPYDRPDAPIVPATVATAHEAGWTWDIGLPSRR
                     GIGYVYSSAHCDDDRAEATLRAYAGPAADGLSARRLRFDTGYRETPWIGNCVAVGLAA
                     GFFEPLESTGVMLIEIAAAMIADLFTPDPDGMAAAARRYNAMMSERHARIIDFLKLHY
                     ALSRRPEPYWRDNADPRSWSETLRDHLAQWARRPVSRFDFAVDHETFLPPSYQFILYG
                     MD"
     gene            complement(1471..3855)
                     /locus_tag="VF402_03425"
     CDS             complement(1471..3855)
                     /locus_tag="VF402_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799372.1"
                     /translation="MKLRTALAATTALICLSATASAFAQAAAPDTSATPKNGSASDDS
                     TVVVVTATKRATKLQDTPIAISAFSQAQLSQNHVRDVTDLAAFVPSVAFTRQGDQAAI
                     MVTMRGIGNDSAFTEVADPEVAIYVDGVYSARAQGASVLMYDMNRVEVLRGPQGTLFG
                     RNATVGAINLITAKPDLSGYSASAEVTMGNYARFGVQGVVNMPLSDTFGLRAAFISDR
                     HDGYIDYQPAPNVPGVNPSAFVTSGKKYYAGDQRSARLSALWKPSDRFTWNLAVEGYQ
                     DTGSPVLALLQTPRAGTKRWSAQIDTAPEQDRHSIALRSQIDWNFSDNVGLTYITGVS
                     RISGGGDSDADVGATPPTVADPNPQGAFNENRTVWSQYDSTSHEVQLKSLGTQTVDWI
                     LGAYYSHEVNAIRFDIDQRNGYRDGTYAWAGSFIQAHREINSSAAFAQGVWHVNDRLR
                     LTAGLRYTQDSKQDVGGRNVTSNCPANTDCSDYPGIFDLAPDATAQQLVAALGQTGQV
                     FAISDNDVKHSWSKMTWLLRADADLWKGTMGYVSVGTGFKGGNIEDGGFTAGPETLTN
                     YEVGTKSTLFGGKATLNLAAYSEDFVGYQVNQVVTLRDASGNTLSTSLRTVNAKGAKA
                     YGFEAEFNARLTPVDHLQITLDAQHTELEDLLTIDNRIYDGADFAHAQQLKGNELPHA
                     PHLAGTIGYDHVFALGSGAAITPRFTTHFEDKSWLSYFNVPTYDEQKAYTKSDLSLKY
                     DEPDRRWSLEAFVLNVEDATIKTNSGTFGPIGAKVWTSVYAPPRTWGVRLRASF"
     gene            4124..>4564
                     /locus_tag="VF402_03430"
     CDS             4124..>4564
                     /locus_tag="VF402_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7799373.1"
                     /translation="MGATIGSALTSLAAQAEAPAPAPPAAARKAGPDARAGALLQRMT
                     RAEKLSLVFGYFGADIPGKYKRPQASRWSSAGYAPGVARLGIPAQWQTDAGIGVATQH
                     DATHWRERTALPSGLGTAATWNTQLAYEGGAMIGAEARDSGFNVL"
BASE COUNT          760 a         1447 c         1595 g          762 t
ORIGIN      
        1 gtccatgccg tacaggatga actgatagct gggcggcaga aaggtctcgt gatcgacggc
       61 gaagtcgaag cgcgacaccg ggcggcgcgc ccattgcgcc agatggtcgc gcagcgtttc
      121 ggaccagctg cgcggatcgg cgttgtcgcg ccagtacggt tccgggcggc ggctcagcgc
      181 atagtgcagc ttgaggaagt cgatgatgcg cgcatggcgt tcgctcatca tcgcgttgta
      241 gcggcgcgcg gcggcggcca tgccgtccgg gtccggcgta aaaagatcgg cgatcatcgc
      301 cgccgcgatc tcgatcagca tgacgccggt cgattccagc ggctcgaaaa agcccgccgc
      361 cagcccgacc gccacgcaat tgccgatcca cggcgtctcg cggtagccgg tgtcgaagcg
      421 cagccgccgc gcgctcaggc cgtccgccgc cggaccggca taggcgcgca gcgtcgcctc
      481 ggcgcggtcg tcgtcgcaat gcgcgctgga atagacatag ccgattccgc gccgcgacgg
      541 caggccgatg tcccacgtcc agcccgcttc gtgcgcggtg gccacggtcg ccggaacgat
      601 cggcgcgtcg ggccggtcgt agggcagttg cagcgccacc gcccggtcat tgaacaggct
      661 gtcgcccgcc ggacggaagc gcgcgccgag cgcctcgccg atcagccggg cgcgaaagcc
      721 ggtgcaatcg agatagagat cggcgctcag atcgccgtga ccggcgacat gcacgcccgc
      781 gatgcgcccg tccggggcca gcgtcacgcc gtccgcctcg ccttgcagat gacgcacgcc
      841 cagcgtccgc gcgcgcgccg ccagcgcctc ggccagcttc gccgcgtcga aatggtaggc
      901 gtagttcatc ggcccggcga agtccgggtc gctgagccgc ttgggggcgc ggccctgacg
      961 cacgacctcg ccctgcaagg tcacggcgtc ggcgaagggc gtcgccgccc ccgccgcgcg
     1021 gcgcaacagc cagtagggcg caagatcgac cggcccggcg cggtgcggcg cgttgaaggg
     1081 atgaaaataa gacgtccccg ccgccttcca gccgtcgaac acgacgccct gcttgaatgc
     1141 cgcgtcggcg tcgcgcagga aggcgcgctc gtccagaccg atgaaggaca aggtgttgcg
     1201 caggctgggg aagctgccct cgccgacgcc gaccgtggcg atggccgggc tttcgaccag
     1261 ggtgatcgcc gcgtcgggca ggctgcgcgc cagataggcc gcgctgagcc agcccgccgc
     1321 gccgccgccc agaatgagga tgcgtgaagg cgccatatcg gctttcccta taaaaatcgg
     1381 gcctatgaaa aaaaagccgg gcggacgcgc cgcccggcga gggaaaaggg agagacgagc
     1441 cggcgcaaca caccggctcg cacaggcagg ttagaacgac gcgcgcaggc gcacgcccca
     1501 ggtgcgcggc ggcgcataga ccgaggtcca gaccttggcg ccgatcgggc cgaaggtgcc
     1561 ggaattggtc ttgatcgtgg cgtcctcgac attgagcacg aaggcttcca gcgaccagcg
     1621 gcggtccggt tcgtcgtact tcaggctgag gtcgctcttg gtgtaggcct tctgctcgtc
     1681 ataggtcggc acgttgaagt aggacagcca gctcttgtcc tcgaagtggg tggtgaagcg
     1741 cggcgtgatc gccgccccgc tgccgagcgc gaagacgtgg tcgtagccga tcgtgcccgc
     1801 caagtgcggc gcgtgcggca gttcgttgcc cttgagctgc tgggcgtggg cgaagtccgc
     1861 gccgtcatag atgcggttgt cgatggtcag caggtcttcc agctcggtgt gctgggcgtc
     1921 cagcgtgatc tgaaggtgat cgaccggcgt caggcgggcg ttgaactcgg cctcgaagcc
     1981 gtaggccttg gcgcccttgg cgttgaccgt gcgcagcgag gtcgacaggg tgttgccgct
     2041 ggcgtcgcgc agggtgacga cctgattgac ctgatagccg acgaagtcct cggaataggc
     2101 ggccaggttc agcgtcgcct tgccgccgaa cagggtcgat ttggtgccga cttcgtagtt
     2161 ggtcagggtt tcggggcccg cggtgaagcc gccgtcctcg atattgccgc ccttgaaacc
     2221 ggtgccgacg ctgacatagc ccatcgtgcc cttccacaga tcggcgtcgg cgcgcaggag
     2281 ccaggtcatc ttgctccagc tatgcttgac gtcgttgtcc gagatggcga acacctgacc
     2341 ggtctggccc agcgcggcga cgagctgctg cgccgtggcg tccggcgcca ggtcgaaaat
     2401 gcccggatag tccgaacagt cggtgttggc cggacagttg gacgtcacgt tgcgcccgcc
     2461 gacgtcctgc ttgctgtcct gcgtgtagcg caggcccgcc gtcaggcgca ggcggtcgtt
     2521 gacgtgccag acgccctgcg cgaaggccgc cgaggaattg atctcgcggt gcgcctgaat
     2581 gaacgaaccg gcccaggcgt aggtgccgtc gcgatagccg ttgcgctggt cgatgtcgaa
     2641 gcggatcgca ttgacctcgt gcgaatagta ggcgcccagg atccagtcga cggtctgcgt
     2701 ccccagcgac ttcaactgca cttcgtggct ggtggagtcg tattgcgacc agacggtgcg
     2761 gttctcgttg aacgcgccct gcgggttggg gtcggcgacg gtcggcggcg tcgcgccgac
     2821 gtcggcgtcg gaatcgccgc caccggagat gcgcgacacg cccgtgatat aggtcaggcc
     2881 gacattgtcg ctgaagttcc agtcgatctg gctgcgcagc gcgatggagt ggcggtcctg
     2941 ctccggcgcg gtgtcgatct gggccgacca gcgtttcgtg cccgcgcgcg gcgtctgcaa
     3001 cagggccaga accggcgagc cggtgtcctg atagccctcg accgccaggt tccaggtgaa
     3061 gcggtcgctc ggcttccaca gggcgctcag gcgggcggag cgctgatcgc ccgcatagta
     3121 cttcttgccc gaggtcacga aggccgacgg gttgaccccc ggcacgttcg gcgcgggctg
     3181 atagtcgata tagccgtcat gccggtcgct gatgaaggcc gcgcgcaggc cgaaggtgtc
     3241 ggacagcggc atgttgacca cgccctgcac gccgaagcgc gcgtaattgc ccatcgtgac
     3301 ctcggcgctg gcgctgtagc ccgacaggtc cggcttggcg gtgatcaggt tgatcgcgcc
     3361 gaccgtggcg ttgcgtccga acagggtgcc ctgcgggccg cgcagcactt cgacgcggtt
     3421 catgtcgtac atcagcacgc tggcgccctg agcgcgcgcc gaatagacgc cgtcgacata
     3481 gatggccact tccgggtcgg cgacctcggt gaaggccgag tcgttgccga tgccgcgcat
     3541 ggtgaccatg atcgccgcct gatcgccctg acgggtgaag gccacgctcg gcacgaaggc
     3601 ggccaggtcg gtgacgtcgc ggacgtggtt ctggctcagt tgcgcctgac tgaaggccga
     3661 gatggcgatc ggggtgtcct gaagcttggt cgcgcgcttg gtcgcggtga cgaccacgac
     3721 ggtcgaatcg tcggaagcgc tcccattctt cggtgtggcg gacgtgtccg gcgcggcggc
     3781 ctgcgcgaag gccgaagccg ttgcggacaa acagataagc gccgtcgtcg ccgccagagc
     3841 ggtgcgtaat ttcattgatt tcctccgttg tattgatccc cccgttatgg gatcgatcgg
     3901 cgacaggacg tattttaaat tgacaacatt gtcaaggatg ctatgtattc accatataca
     3961 tcgatcatgc agcatataca ctaaatatag tgtgtaaatg gatgaataat ggatcgtttt
     4021 ccagaattat ccccgatgta aatagaatat acatgaaata aacggtatcg gaacggatat
     4081 gcaaaagagc tggaaatggt gcaaagcggc cacagtggcc atcatggggg ctacaattgg
     4141 aagcgctctc acatcgcttg ccgcgcaagc cgaagcgccc gctccggcgc ccccggcggc
     4201 ggcccggaag gccggtcccg acgcgcgcgc cggggcgctg ctgcaacgca tgacgcgggc
     4261 ggaaaagctt tcgctggtct tcggttactt cggcgccgac attccgggta agtataaacg
     4321 cccgcaggcg tcgcgctggt cctcggcggg ctatgcgccg ggcgtggcgc ggctggggat
     4381 tccggcgcaa tggcagaccg acgcgggcat aggcgtggcg acccagcacg acgcgaccca
     4441 ctggcgcgag cgcaccgccc tgccctccgg cctcggcacg gcggcgacgt ggaacacgca
     4501 actggcctat gagggcggcg cgatgatcgg cgccgaggcg cgcgattccg gcttcaacgt
     4561 cctg
//
LOCUS       DASVLL010000209         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_12648, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000209 DASVLL010000000
VERSION     DASVLL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12648"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VF402_03435"
     CDS             complement(<1..439)
                     /locus_tag="VF402_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7799374.1"
                     /translation="MRRLLPVIALFALAASARAQDLPQDLPQDLPQPAAQDFKVGALT
                     VTALHDDDFSPANDGKTFAVGQSPDAVAQVLSAHGAPTAAIHMSVDALLLHDGGRVVL
                     IDTGLGYRYHGVLLGSLAKAGVAPEAVTDVLITHAHPDHIGGLV"
     gene            594..1562
                     /locus_tag="VF402_03440"
     CDS             594..1562
                     /locus_tag="VF402_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7799375.1"
                     /translation="MHDHAHSHHPDHGHGHSHGHSHGGGHGHSHAPADFGRAFLIGIV
                     LNSVFILAEVVYGLTAHSLSLLADAGHNLSDVLGLFMAWGATALAKQPPSARYTYGLR
                     GASIMAALGNAVFLLVAIGGIGWEAVLRLMHPQAAQGLTVMIVAGIGILVNGATAFLF
                     MSGVKGDLNIRGAFLHMLSDAVVSLGVVIAGGVILVTGWLWLDPLVSLIIGAVILWGT
                     WGLLRDSVKLSLHGVPPGIELQKIRALVAGRPGVSEVHDLHVWGMSTTETALSAHIVM
                     PGGHPGDGFLHEMAHDLEHLGVQHVTIQIEVADGGQPCHSCPDTNV"
     gene            1628..>2194
                     /locus_tag="VF402_03445"
     CDS             1628..>2194
                     /locus_tag="VF402_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7799376.1"
                     /translation="MSDLDKIIRRSEPGAVLGEGPLWSKRDQCVYWVDILSNKLHACY
                     LDGRARTWDFPEHVTWVIERDISAFPGGGFIAGLMSGFAEVTLEPFSIKHIANPYPHE
                     PENRMNDAKADDRGRIWAGSMHKPITKTSGAFYRLNADLSVDEVDGPYKIANGPTFSA
                     DHSKVWHTDTGKSEIYLFDVIDGELANKR"
BASE COUNT          388 a          694 c          722 g          390 t
ORIGIN      
        1 tcacgaggcc gccgatatga tcgggatgcg cgtgggtgat cagcacgtcg gtgaccgctt
       61 cgggcgcgac gcccgccttg gcgaggctgc ccagcagcac gccgtgatag cgatagccga
      121 ggcccgtgtc gatcagcacg acgcggccgc cgtcgtggag cagcagcgcg tcgacgctca
      181 tatggatcgc cgccgtgggc gcgccgtgcg cgctcagcac ctgcgccacc gcgtccgggc
      241 tttggccgac ggcgaaggtc ttgccgtcat tggccgggct gaaatcgtca tcgtgcagcg
      301 ccgtgacggt gagcgcgccg accttgaaat cctgcgcggc aggctggggc aagtcctggg
      361 gcaagtcctg gggcaagtcc tgcgcccgcg ccgaagcggc gagggcgaac agggcgataa
      421 cgggaagaag gcggcgcatg atgaagctcc gtgatttggc catgatgcgg ccatgatcgg
      481 gttatgagat aacgtacttc aaacgcaata aaaacaagac tttattttga gaattcattt
      541 gaccggcggc gcgcaatttg tcaatccgtc ggacatgagc cggagccgcc gccatgcacg
      601 atcacgcgca ttcccatcac cctgatcacg gacacggcca ttcgcatggc cattctcacg
      661 gcgggggaca cggccacagc catgcgccgg ccgatttcgg cagggccttc ctgatcggca
      721 tcgttctcaa cagcgtgttc attctggccg aggtcgtcta tggcctgacc gcccattccc
      781 tgtcgctgct ggccgacgcc gggcataatc tgtcggacgt gctgggcctg ttcatggcgt
      841 ggggcgcgac ggcgctggcg aaacagccgc ccagcgcgcg ctacacctac ggcctgcgcg
      901 gcgcctcgat catggcggcg ctgggcaatg cggtgtttct gctggtcgcc atcggcggca
      961 tcggctggga ggcggtcctg cgcctgatgc acccgcaggc cgcgcaaggg ctgacggtga
     1021 tgatcgtggc ggggatcggc atcctcgtca acggcgccac cgccttcctg ttcatgagcg
     1081 gcgtcaaggg cgacctcaat atccgcggcg ccttcctgca catgctgtcc gacgcggtgg
     1141 tgtcgctggg cgtggtgatc gcgggcgggg tgattctggt caccggctgg ctgtggctcg
     1201 atccgctggt cagcctgatc atcggcgcgg tcatcctgtg ggggacgtgg ggactcttac
     1261 gcgactcggt caagctgtcg ctgcacggcg tgccgccggg gatcgagttg cagaagatcc
     1321 gcgccctggt cgcgggcagg ccgggggtga gcgaggtcca cgacctgcac gtctggggca
     1381 tgagcacgac cgagacggcg ctgtcggcgc atatcgtcat gccgggcggc catccgggcg
     1441 acggcttcct gcacgagatg gcgcacgatc tcgaacatct cggcgtgcag cacgtcacga
     1501 tccagatcga ggtcgccgac ggcggccagc cgtgtcattc ctgcccggac accaacgtat
     1561 aaatccatat tgtatgattt tatatcctgc ggtaagacgc cggtaaaatc agcgacaggg
     1621 aaacagcatg agcgacctcg acaagatcat ccgccgttcc gagccgggcg ccgttctggg
     1681 cgaagggcct ttatggtcga agcgcgatca gtgcgtctat tgggtcgata tcctgtcgaa
     1741 caagctgcac gcctgctacc tcgacggccg ggcgcggacg tgggacttcc ccgaacacgt
     1801 cacctgggtg atcgagcgcg acatttctgc ttttcctggg ggcggcttca tcgccgggct
     1861 gatgagcggt ttcgccgagg tcacgctgga gccgttcagc atcaaacaca tcgccaaccc
     1921 gtatccgcac gagccggaaa accgcatgaa cgacgccaag gcggacgacc gtggccgcat
     1981 ctgggccggt tcgatgcaca agccgatcac caaaacgtcc ggcgccttct accgcctgaa
     2041 cgccgatctg tcggtcgatg aggtcgacgg cccgtacaag atcgccaacg gcccgacctt
     2101 ttccgccgac catagcaagg tctggcacac cgacaccggc aagtcggaaa tctacctctt
     2161 cgacgtcatc gacggcgaac tggccaacaa gcgt
//
LOCUS       DASVLL010000210         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_119691,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000210 DASVLL010000000
VERSION     DASVLL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119691"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VF402_03450"
     CDS             <1..546
                     /locus_tag="VF402_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX7799377.1"
                     /translation="IYVVEPRAGARGEMEDLDVGAFELLEARIIIEGGAAAVAATAAS
                     EADIARLAGLLDAMERSAAAQSEALDREFHLELARLTRNGPLLDAIETLWDLRTKSKL
                     ARMIERRAHGGGRLQRDEEHRAILDALTRRDPAGARAAMQSHLEKVRDFLLESTETEE
                     IQKLRDQQRQAREALTRRSVY"
     gene            965..1507
                     /locus_tag="VF402_03455"
     CDS             965..1507
                     /locus_tag="VF402_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7799378.1"
                     /translation="MDLSSQTLPAGRPLTAEWVYRRYAKAIRQYFLKRVRNPAEAEDL
                     TSELLLRVSQNADRSVQNPEGFIFSIAANLLRDRYRHQQVVDRYRRDQAAPAAGIEVL
                     SPERVLTGKQSLDALMRALDGLDARARNVFVLSRIDGMSYAGIARLLGLSVSSIEKDM
                     MRALAALSRHVAQSQEQDGF"
     gene            1504..>1747
                     /locus_tag="VF402_03460"
     CDS             1504..>1747
                     /locus_tag="VF402_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4880 domain-containing protein"
                     /protein_id="HEX7799379.1"
                     /translation="MTGQDAHNRPQGTPDEAAASWWMARETGHHFDPAGFEAWLAADP
                     ENAAAWERVRAIWQGFGDHRAAPELAEARLDARRWAR"
BASE COUNT          316 a          551 c          609 g          271 t
ORIGIN      
        1 atctatgtcg tcgaaccgcg cgccggagcg cgcggcgaga tggaggatct cgacgtcggc
       61 gccttcgagc ttctggaagc gcgcatcatc atcgagggcg gcgcggcggc ggtggccgcg
      121 acggcggcgt cggaagcgga catcgcgcgg ctggccgggc tgctcgacgc gatggagcgc
      181 agcgcggcgg cgcagtcgga ggcgctggac cgcgaatttc acctcgaact ggcgcggctg
      241 acgcgcaacg ggccgctgct cgacgcgatc gagacgctgt gggatctgcg cacgaaatcg
      301 aagctggcgc gcatgatcga acggcgcgcg cacggcggcg ggcggctgca acgcgacgag
      361 gagcatcgcg ccatcctcga cgccctgacg cgccgcgacc cggccggggc gcgcgcggcg
      421 atgcagagcc atctggaaaa ggtgcgcgac ttcctgctgg aatcgaccga gaccgaggaa
      481 atccagaaac tgcgcgacca gcagcgtcag gcgcgcgaag cgctgacgcg gcggtcggtg
      541 tattgatggc cgtatagcgg gtcggactgg ctataagaag ggcccctctc accccctccc
      601 ttcacgaaaa gcccccggag cctttcgtga actcgacccc gctgcgcagg gagggcgatt
      661 ctcctttggc cttccccgtt cacggggaag gtggcatttg cggagcaaat gacggaaggg
      721 ggtctttgca atcgaggctg caaggcgcgg ttgtgatcga atgtgaacat tcgcgcatcg
      781 gcttctaatg ctttaattac aagccttatc gcacgaccac ccccaccccg gccctgagtc
      841 gccaatcaaa acgcttgctc taatttgcgc gttttgctac aaaataatca aaaaagatca
      901 tgcgccggag cgtcgcgcgc aagacaacac acgaggcaac tcaggggcca aggaagcaaa
      961 gggtatggac ctgtccagtc agactctgcc cgcgggccgt ccgctgacgg cggaatgggt
     1021 gtaccggcgc tacgccaagg cgatccggca gtatttcctc aagcgggtgc gcaaccccgc
     1081 cgaggccgag gatctgacgt ccgagctttt gctgcgcgtg tcgcagaacg cggaccgcag
     1141 cgtgcagaat cccgaaggct tcatcttcag catcgccgcc aacctgctcc gggaccgtta
     1201 ccgccatcag caggtcgtcg accgctaccg ccgcgatcag gccgcgcccg ccgccggaat
     1261 tgaggtcttg tcgcccgagc gcgtcttaac gggcaagcag tcgctcgacg ccctgatgcg
     1321 cgcgctggac ggacttgacg cgcgcgcgcg aaacgtcttc gttctgagcc ggatcgacgg
     1381 tatgagctat gccgggatcg cgcgcctgtt ggggttgtcg gtctcctcga tcgaaaagga
     1441 catgatgcgc gcgctggcgg cgctgtcgcg ccatgtcgcg caaagtcagg aacaggacgg
     1501 gttttgacgg gtcaggacgc gcataacagg ccgcagggca cgcccgacga agcggcggcg
     1561 tcgtggtgga tggcgcgcga gacgggccat catttcgacc cggcgggctt cgaggcgtgg
     1621 ctggccgccg atcccgaaaa cgcggcggcg tgggagcgcg tgcgcgcgat ctggcagggc
     1681 ttcggcgatc accgcgccgc gccggaattg gctgaggcgc ggctggacgc acggcgctgg
     1741 gcgcgcg
//
LOCUS       DASVLL010000211         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_174085,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000211 DASVLL010000000
VERSION     DASVLL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174085"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VF402_03465"
     CDS             <1..526
                     /locus_tag="VF402_03465"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX7799380.1"
                     /translation="TVPLATVSACDLLCVPGGLAATEVALDRGFVAQIRRLGLGARYV
                     TSVCTGALILGAAGLLIGKRAACHWAWRDLLPLFGAIPEADRIVRDGDVITGGGVTAG
                     IDFALAVLAEISGADVAEAIQLGLEYDPQPPFRSGSPQSARPEVLARWRAAAEPMMEK
                     RRAEAAEAARRLGG"
     gene            complement(619..>1558)
                     /locus_tag="VF402_03470"
     CDS             complement(619..>1558)
                     /locus_tag="VF402_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7799381.1"
                     /translation="PKDYEQAAKYFAYAAQRGVANAQKNLGVMYMNGFGAPRNPEEAV
                     KWFRLAAQQGDPIAEYELGAAYARGVGIQQDSAEAARWYKRSADQDYPRAENNLGVMY
                     LFGQGVAEDDLMAVTLFKRSVAHGNALSERNLGFAYARDGGPAQNYVEALRWYRRAAD
                     HGDIKALSDVGAIYATGRGVKQDYGEAQKWYQRAADRGDSGAAQIVQHIEAARAAQSA
                     GAAEAEAARAAARNNAFAPTPTIARSATAPQRVAASSPPRGGWTPMDPAVFRFSSPMA
                     EHHSSSIGAAFAPHLTAPIFGGGGHAAFAAGFHGRR"
BASE COUNT          242 a          523 c          495 g          298 t
ORIGIN      
        1 tacggttccg ctcgccaccg tctccgcttg cgatcttctc tgcgttccgg gcgggcttgc
       61 agcgacggaa gtcgcgctcg atcgcggctt tgtcgcgcag atccgccgcc tgggccttgg
      121 ggcgcgctat gtgacgtccg tttgcaccgg cgcgctcatc cttggcgctg cgggtcttct
      181 gatcggcaag cgcgcggcct gtcattgggc ttggcgcgat cttctgccgc tcttcggcgc
      241 cattccggaa gccgatcgga ttgttcgcga cggcgatgtg atcaccggcg gcggcgtcac
      301 ggccgggatc gatttcgccc tcgccgttct cgcagaaata tccggcgctg acgtcgctga
      361 agctatccaa ctcggcctcg aatatgaccc gcagccgcct ttccgctctg ggtctcccca
      421 atcggcgcgg cctgaagtcc tcgcccgctg gcgggctgca gccgaaccga tgatggagaa
      481 gcgtcgcgcc gaggccgcgg aagccgcgcg ccgtctcggc gggtaacaac tggaaacgac
      541 ctatagggcc tcccgggtgt ccccggtcag ctcaaagtaa ttcctccacg cgacgctcaa
      601 tcagcggaca agcagagatc accgccttcc gtgaaagccg gccgcgaatg cggcatgacc
      661 gccaccgccg aaaatgggcg ctgtcagatg cggcgcgaaa gccgcgccga tcgatgaaga
      721 atgatgctcg gccatgggcg aagagaaccg gaagaccgca gggtccatcg gcgtccagcc
      781 gccgcgcggc ggagatgacg cggcaacccg ctgcggggct gtggcgcttc ttgctatggt
      841 cggcgtcggc gcaaaggcgt tgttccgcgc ggcggcgcgg gctgcctccg cctccgccgc
      901 gcctgcggac tgcgccgctc gcgccgcctc tatatgttgg acgatctggg ccgctccgga
      961 atcaccccga tcagctgcgc gctggtacca tttctgcgcc tcgccgtaat cctgcttcac
     1021 cccgcggccc gtcgcataga tggcgccgac gtccgacagg gccttgatgt ccccatgatc
     1081 ggcggcgcgg cgataccatc tgagcgcctc gacgtaattt tgcgcggggc cgccatcgcg
     1141 cgcataagcg aagccgagat tcctttcgga gagggcgttt ccatgcgcga cggagcgctt
     1201 gaacagggtc acggccatga ggtcgtcttc agcaacgccc tggccaaaca gatacattac
     1261 gccgaggttg ttctccgctc tcggatagtc ctggtcggcg gaacgcttgt accagcgtgc
     1321 ggcttccgcg ctgtcttgtt gaatgccgac gcccctggcg taagctgcgc caagctcata
     1381 ttcggcgatg ggatcccctt gctgcgccgc gaggcggaac cacttcaccg cttcctcggg
     1441 attccgcggc gcgccgaagc cgttcatata catgacgccg agatttttct gggcgttggc
     1501 cacgccacgt tgggcggcgt aagcaaaata ttttgcagcc tgctcgtaat ccttcgga
//
LOCUS       DASVLL010000212         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_281621,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000212 DASVLL010000000
VERSION     DASVLL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281621"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VF402_03475"
     CDS             complement(<1..551)
                     /locus_tag="VF402_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7799382.1"
                     /translation="MPHPDRRDILIGASALALLPAISAPARAQTAGDDDRRVSFDDGW
                     RFAFGHLDDVDKDFGWGKDQRTYAKQGPDSGAAAQAGFDDSGWQAVNLPHDWAVALPF
                     VHKEPNPPLKPDAHGAVWDGPANHGYKPLGRDYPETSVGWYRKTFSLTPADKDRKVFL
                     EFDGVFQACAVMVNGYIVREHTGG"
     gene            complement(649..>2047)
                     /locus_tag="VF402_03480"
     CDS             complement(649..>2047)
                     /locus_tag="VF402_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799383.1"
                     /translation="KPTVSVSSAPEDNWQTGDAGQWQTGHVKDTLSAVQADATRHFGD
                     GVLTAVSFGARYADRKKALGSASGTVAALSGAGIDGGDLTAYHFDNFDIPTMLTGDFD
                     SIAQTLYGQKLSYDPKAYPDTDRVEEKVAEAYIEGLYATTMGDIPVDGNLGVRVLNVH
                     TDSHGTSTVAGDWVETPPGSGTWVQQMITTPVSGGTNYTRLLPSFQMRFDFGNGHYLK
                     AAAAQVVSRPPLNDMIITRQITSTAPYTGSSGNPYLMPFEATQFDLSYEWYFSKDSLF
                     ALAGYYKNVANYVGYATRAETIDGHPYTLTSPVNASKGGHIAGTELTFQTPFYFIPGG
                     EHFGVYSNWAYVASDLREMSANLPMNGLAKNTATLDLWYSNHGIDARLGTKYHSTYTA
                     IYGWDDTALVRVRPETTMDFSIGYTINKHVQVRFQAYNMLNTPLKTYEDNQIDRLGRW
                     DYYGRSYLADVTFRY"
BASE COUNT          322 a          628 c          701 g          396 t
ORIGIN      
        1 ccgcccgtat gttccctgac gatatagccg ttgaccatga ccgcgcaggc ctgaaacacg
       61 ccgtcgaatt cgagaaacac cttcctgtct ttgtcggcgg gcgtcaggct gaaggtcttg
      121 cggtaccagc cgacgctggt ttcaggatag tcgcggccca gcggcttata gccgtgattg
      181 gccgggccgt cccagaccgc gccgtgcgcg tcgggtttga gcggcggatt cggttcctta
      241 tgcacgaagg gcagggcgac ggcccagtcg tgcggcaggt tcacggcctg ccagccggaa
      301 tcgtcgaacc cggcctgcgc cgccgcgccg gaatccggcc cctgcttggc gtaggtgcgc
      361 tggtccttgc cccagccgaa atccttgtcc acgtcgtcga gatggccgaa ggcgaagcgc
      421 cagccgtcgt cgaagctgac ccgccggtcg tcgtcgcccg ccgtctgcgc gcgcgccggg
      481 gcggatatgg cgggcagcag ggccagggcg gaggcgccga tgagaatgtc gcggcggtcg
      541 ggatgcggca tggtggattt cccctttttg tatgatttat tcggatcgca tagaaaaggg
      601 ccgggccttg catgtcaagg cccggccctt tcgccccccg caggcttttt aataccggaa
      661 cgtgacgtcc gccagatagg agcggccgta atagtcccag cggcccagac ggtcgatctg
      721 gttgtcctca taggtcttga gcggcgtgtt cagcatgttg tacgcctgaa aacggacctg
      781 aacgtgtttg ttgatcgtat agccgatcga gaagtccatc gtcgtttccg gccgcacccg
      841 caccagcgcc gtatcgtccc agccgtagat ggcggtgtag gtcgaatggt acttggtccc
      901 cagccgcgcg tcgatcccgt ggttggagta ccacaggtcc agcgtggcgg tgttcttggc
      961 cagcccgttc atcggcaggt tggcgctcat ctcgcgcagg tccgaggcga cataggccca
     1021 gttggaatag acgccgaaat gctcgccgcc ggggatgaag tagaacggcg tctggaaggt
     1081 cagctcggtc ccggcgatat ggccgccctt gctggcgttg accggcgagg tcagggtgta
     1141 gggatggccg tcgatcgtct cggcgcgcgt cgcatagccg acatagttcg cgacgttctt
     1201 gtaataaccg gccagggcga acagcgaatc cttgctgaaa taccattcgt agctgaggtc
     1261 gaactgcgtc gcctcgaacg gcatcaggta gggattgccg gacgagccgg tatagggcgc
     1321 cgtcgacgtg atctggcgcg tgatgatcat gtcgttgagc ggcgggcgcg acacgacctg
     1381 cgcggcggcg gccttcaggt aatggccatt gccgaagtcg aagcgcatct ggaacgacgg
     1441 cagcaggcgg gtgtagttcg tcccgcccga caccggcgtg gtgatcatct gctgcaccca
     1501 cgtgccgctg cccggcgggg tttcgaccca gtcgcccgcc acggtcgagg ttccgtggct
     1561 gtcggtatgc acgttcagca cgcgcacgcc cagattgccg tcgaccggaa tatcgcccat
     1621 cgtggtggcg tacaggccct cgatataggc ttcggcgacc ttttcctcga cgcggtcggt
     1681 gtcgggatag gctttcggat cgtagctcag cttctggccg tacagggtct gggcgatgga
     1741 gtcgaaatcg ccggtcagca tggtcgggat gtcgaaattg tcgaagtggt aggcggtcag
     1801 gtcgccgccg tcgatgcccg ccccggacag ggcggccacc gtgccggacg cgctgcccag
     1861 cgccttcttg cggtcggcgt agcgcgcgcc gaagctgacc gcggtcaaaa cgccgtcgcc
     1921 gaagtggcgc gtggcgtcgg cctgcacggc gctgagcgtg tccttgacat ggccggtttg
     1981 ccactggccc gcgtcgccgg tctgccagtt gtcctccggc gcggacgaga cgctgaccgt
     2041 cggcttg
//
LOCUS       DASVLL010000213         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_281758,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000213 DASVLL010000000
VERSION     DASVLL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281758"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VF402_03485"
     CDS             complement(<1..930)
                     /locus_tag="VF402_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7799384.1"
                     /translation="MSQNFHSPATHGFVRVAAVTPRVHIGDPAANLREHVELARRCGA
                     AGADVVVYPELGLSAYALDDLFLQDTLLDAARAALEGLIQASEGLRPVLAAGAPLKVG
                     DAVYNCAVVIHDGRALGVVPKTFLPNYREFYEKRWFAPGADLTADSVELAGRSVAAGT
                     DLLFQAHGLPDFRLGVEICEDMWSPVTPSTGLALGGATLIVNLSASPVTVGKSRTRKA
                     LCAAISERLFCAYAFSASGPGESTTDLAWDGQSLIYELGALLAEGERFASDTITLADA
                     DIGRVAAERLRTGTFNDARNDARRRQGATARTVT"
     gene            1163..>2036
                     /locus_tag="VF402_03490"
     CDS             1163..>2036
                     /locus_tag="VF402_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7799385.1"
                     /translation="MPAVTFADIEAAAERIAGRIVPTSLPFSQRLSEAFGQQLWIKAE
                     NRQYTSSFKERGALNKLMLLSETERRRGVFAASAGNHAQGLAYHATRLGVPSTIVMPE
                     GTPFVKIERTQSFGAKVVIEGASYDEASAAAQALCAGQGGVYVHPFDDPAVIAGQGTI
                     GLEMLTQAPELDTLVAPIGGGGLIAGIAIAAKTMNPAIRVIGVEAAMYPSFHARRHGL
                     PNPAGGVTIAEGIAVKAVGDLTFALADPLVDEALLLDEADFERGVAAFANIEKTVAEG
                     AGAAALAAVMRYPEL"
BASE COUNT          344 a          711 c          681 g          300 t
ORIGIN      
        1 ggtcaccgtg cgcgccgtcg cgccctgacg gcggcgcgcg tcgttgcgcg cgtcgttgaa
       61 cgtgccggtg cgcagccgct ccgccgccac gcggccgata tcggcgtcgg ccagcgtgat
      121 cgtatcggag gcgaaccgct cgccctcggc cagcaacgcg cccagttcgt agatcagcga
      181 ctgcccgtcc caggccaggt cggtggtcga ttcgcccggc cccgacgcgc tgaacgcata
      241 ggcgcagaac aggcgctcgg atatcgccgc gcacagggct ttacgcgtgc gcgacttgcc
      301 gaccgtgacc ggcgaggccg acaggttgac gatcagcgtc gcgccgccga gcgccagacc
      361 ggtcgagggc gtcaccggcg accacatgtc ctcgcagatc tcgaccccca gccggaagtc
      421 gggcaggccg tgcgcctgaa acagcaggtc cgtccccgcc gccacgctcc gtccggccag
      481 ttcgacgctg tccgccgtca ggtcggcgcc gggcgcgaac cagcgctttt cgtagaattc
      541 gcggtaattg ggcaggaagg tcttgggcac gacgccgagc gcgcgaccgt catgaatgac
      601 gacggcgcaa ttatagaccg cgtcgccgac cttcagcggc gcgcccgccg ccagcaccgg
      661 acgcaggccc tcggacgcct gaatcagccc ctccaacgcc gcgcgcgccg cgtccagcag
      721 cgtgtcctgc aagaacaggt cgtcgagcgc ataggccgac aggcccagtt cgggataaac
      781 gaccacatcc gcgcccgccg cgccgcagcg ccgcgccagt tcgacatgtt cgcgcaggtt
      841 cgccgccgga tcgccgatat gcacgcgcgg cgtgacggcg gcgacgcgca cgaagccatg
      901 tgtggcgggc gaatggaaat tctggctcat ttcggcgctt tcctcgacat cgaccgctga
      961 aacttagtcg ctgagggctt aaaattcaaa gccgaaacgc cgaaacgccg aaaatcggcg
     1021 cgaaaatgcg cagattcgca tagcgcaatc gacaggacgg ccttattggt atttattgaa
     1081 accatatcgc gcccgcattt tttgcgcgcc gggcgggtgc ggtaatgcta agaggacggc
     1141 ccgcatcgac ctgagtcttg acatgcccgc cgtcaccttc gccgatatcg aggccgccgc
     1201 cgaacggatc gcgggccgga tcgtgcccac ctccctgccg ttctcgcagc gcctgagcga
     1261 agccttcggg cagcaattat ggatcaaggc cgagaaccgg caatacacct ccagcttcaa
     1321 ggagcgcggc gcgctcaaca agctcatgct gctgagcgag acggaacggc ggcgcggcgt
     1381 gttcgcggcc tcggcgggca atcacgcgca gggtctggcc tatcacgcca cgcggctggg
     1441 cgtccccagc accatcgtca tgcccgaagg cacgccgttc gtgaagatcg agcgcacgca
     1501 aagcttcggc gcgaaggtgg tgatcgaggg cgcctcctac gacgaagcct cggcggcggc
     1561 gcaggcgctg tgcgccgggc agggcggcgt ctatgtccat ccgttcgacg atcccgccgt
     1621 gatcgcgggg cagggcacga tcgggctgga gatgctgacc caggcgccgg agctggacac
     1681 gctggtcgcg ccgatcggcg gcggcgggct gatcgccggg atcgccatcg ccgccaaaac
     1741 catgaatccc gccatccgcg tcatcggcgt cgaggcggcc atgtacccgt ccttccacgc
     1801 gcgccgccac ggcctgccca atccggcggg cggcgtcacc atcgccgagg gcatcgcggt
     1861 caaggcggtc ggcgacctga ccttcgcgct ggccgacccg ctggtcgacg aggcgctgct
     1921 gctggacgag gccgatttcg agcgcggcgt cgccgccttc gccaatatcg aaaagaccgt
     1981 cgccgaaggc gcgggcgcgg cggcgctggc ggcggtgatg cgctatcctg agcttt
//
LOCUS       DASVLL010000214         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_334905,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000214 DASVLL010000000
VERSION     DASVLL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334905"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF402_03495"
     CDS             <1..292
                     /locus_tag="VF402_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7799386.1"
                     /translation="QWSLDSDRRVLAQAFYEDLTEDARPGLPALTAPVDVIYPVDAAQ
                     TPEATAAFYKAAWAGAPRVAFTPIAASRHFEMLDQPAAFYGALDGALDAALK"
     gene            392..1780
                     /locus_tag="VF402_03500"
     CDS             392..1780
                     /locus_tag="VF402_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HEX7799387.1"
                     /translation="MPPETTPQTDKTALQAYEPIRPLEIASYSLGDAGFNLYWAPITA
                     FLMIYLSDVAGISMAAIALLLLVMRLVSAVAEPVFAAIADRTDTAYGRYRPWFLWLGL
                     PMAAAGIATFSSGALVPEQRLPAIAFGLIGLNLIYSAANVAYNALSGVITPDSAQRDT
                     ILSWRYGTAFLGAVAVTWLTPKLIDWAGRGDHALGWQFTMTVYGVAAVGCLIMVFLNT
                     RERFSLATQPRPNPLRDIADLFCSRPWIVLFALGAVETAAFMLHTSATPYFIKYVAGR
                     ADLVGGFMMVFYVGLAAGSGACSTLTRFATRQVWIAVMLGVAALVGLALYLCPGRALT
                     VLFVLQGLSGAAFGVVSTLSFAMYADAADYNAWKTGYRATAMTYSMINVGKKIAAAAA
                     GAALGWLLSRHGYVAGDAPQAALSQMRAALGLFPAALCVLGIAVILIYDLTPAKVSRL
                     QAQLLGRFMQKT"
     gene            complement(1784..3229)
                     /locus_tag="VF402_03505"
     CDS             complement(1784..3229)
                     /locus_tag="VF402_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7799388.1"
                     /translation="MAKTILICDDDPTQRRLIQAVLEREGFSVMHAENGAQAYERLTQ
                     NSGIDAVILDLVMPGQSGIDTLKDIRAAGVRTPVVVLTASGGIDTVVKAMQAGAQDFF
                     IKPASPERILVSVRNALQMGDLSAEVQRLQKRNDNRASFDDLIGSSPAMQMVRRLGER
                     AAKSNIPVLILGESGVGKELIARAIQGASDRAGKPFVAVNCGALPENLVESILFGHEK
                     GSFTGASDKHLGKFQEANGGTLFLDEVGELPLDMQVKLLRALQEGEIDPVGGKRPVKV
                     DVRIISATNRNLADQVRAGAFREDLFYRLNVFPLEAPALRDRREDIPALVDHFVRRFN
                     VEEGKRVTGVSAEAMQMLSAYDWPGNVRQLENAVYRAIVLADSPYLQPFDFPAISGQS
                     APLAASGAVHTAIAEAHEAISALHDAPVRILDEAGHLRKLEEIERDLIEHAISVYSGH
                     MSEVARRLGIGRSTLYRKVRDCGLENLVKES"
BASE COUNT          497 a         1157 c         1096 g          594 t
ORIGIN      
        1 gcaatggtcg ctggactccg accgccgcgt gctggcgcag gccttctacg aggatctgac
       61 cgaggacgcg cgccccggcc tgcccgcgct gacggcgccg gttgacgtca tctatccggt
      121 ggacgccgcg cagacgcccg aagcgaccgc cgccttctac aaggccgcct gggccggcgc
      181 gccgcgcgtc gccttcacgc cgatcgccgc cagccgccat ttcgagatgc tggatcaacc
      241 cgccgccttt tacggggcgc tcgatggggc gctcgatgca gcgctgaaat gaaccgcgtt
      301 cgccggaggc ccacaacctc cggctttacg gtttcattaa gacctgcggc ggcacaatcg
      361 cgcatccccg cgggaacgaa tccgaagcgc catgccgccc gaaacaacgc cccagaccga
      421 caagaccgcg ttgcaggcct atgaaccgat ccgcccgctg gagatcgcca gctacagtct
      481 gggcgacgcg ggcttcaacc tgtactgggc gccgatcacc gccttcctga tgatctatct
      541 gagcgacgtg gcgggcatat cgatggccgc catcgcgctg ctgctgctgg tcatgcggct
      601 ggtcagcgcg gtggccgagc cggtcttcgc cgccatcgcc gaccgcaccg acaccgccta
      661 tggccgctat cgcccgtggt tcctgtggct gggcctgccg atggcggcgg cggggatcgc
      721 gaccttttcc agcggcgcgc tggtcccgga acagcgcctg cccgccatcg cgttcggcct
      781 gatcggcctc aacctgatct acagcgccgc caatgtcgcc tacaacgccc tgtccggcgt
      841 catcacgccc gattcggcgc agcgcgacac catcctgtcg tggcgctacg gcacggcctt
      901 tctcggcgcc gtcgccgtga cctggctgac gccgaagctg atcgactggg ccgggcgcgg
      961 cgatcacgcg ctgggctggc agttcaccat gacggtttac ggcgtcgccg cggtcgggtg
     1021 cctgatcatg gtgttcctca acacgcgcga gcgcttctcg ctggcgacgc agccgcgccc
     1081 caatccttta cgcgacatcg ccgacctgtt ctgcagccgc ccgtggatcg tgctgttcgc
     1141 gctcggcgcg gtcgagacgg cggccttcat gctgcacacc tcggccacgc cctatttcat
     1201 caaatacgtc gccgggcgcg ccgacctggt cggcgggttc atgatggtgt tctatgtcgg
     1261 cctggccgcc gggtcgggcg cctgctcgac cctgacgcgc ttcgccacgc ggcaggtgtg
     1321 gatcgcggtc atgctggggg tcgcggcgct ggtcgggctg gccctgtacc tgtgtccggg
     1381 gcgcgccctg accgttttgt tcgtgttgca gggcctgtcg ggcgcggcgt tcggcgtcgt
     1441 ctcgaccctg agcttcgcca tgtacgccga cgccgccgac tacaacgcct ggaagaccgg
     1501 ctatcgcgcc acggccatga cctattccat gatcaatgtc ggcaagaaga tcgccgccgc
     1561 cgccgcgggc gccgcgctgg gctggctgct gtcgcggcac ggctatgtcg ccggggacgc
     1621 gccgcaggcc gcgctgagcc agatgcgcgc ggcgctgggc ctgtttcccg ccgcgctgtg
     1681 cgtgctgggg atcgccgtca tcctgatcta cgacctgacc ccggccaagg tcagccgtct
     1741 gcaggcgcag ttgctgggcc gcttcatgca gaagacttga tcgtcacgac tccttgacga
     1801 ggttctccag accgcagtcg cgcaccttgc ggtacagggt cgagcgtccg atgccgagac
     1861 ggcgcgcgac ttccgacatg tggccggaat agacgctgat cgcgtgttcg atcaggtcgc
     1921 gctcgatctc ctccagcttg cgcagatggc ccgcctcgtc gaggatgcgc accggcgcgt
     1981 cgtgcagggc gctgatcgcc tcgtgcgcct cggcgatcgc cgtatgcacc gccccggatg
     2041 cggccagagg cgccgactgg ccgctgatcg cggggaagtc gaacggttgc agatacgggc
     2101 tgtcggccag cacgatggcg cgatagacgg cgttttcgag ctggcgcaca ttgccgggcc
     2161 agtcgtaggc gctcagcatc tgcatcgcct cggcggacac gccggtgacg cgcttgccct
     2221 cctcgacgtt gaagcggcgc acgaaatgat cgaccagagc cggaatgtcc tcgcgacggt
     2281 cgcgcagggc gggggcttcg agcggaaaca cgttcaggcg atagaacaga tcctcgcgga
     2341 aggcgcccgc gcgcacctga tcggccagat tgcgattggt cgccgagatg atgcgcacat
     2401 cgaccttgac cggacgcttg ccgccgaccg gatcgatctc gccttcctga agtgcgcgca
     2461 gcagcttgac ctgcatgtcg agcggcagtt cgccgacctc gtcgaggaac agagtgccgc
     2521 cgttggcctc ctgaaacttg cccagatgct tgtccgaagc gccggtgaac gaaccctttt
     2581 cgtggccgaa caggatggat tcgacgagat tttccggcag ggcgccgcag ttgaccgcca
     2641 cgaacggctt gcccgcgcgg tcgctggcgc cctgaatggc gcgcgcgatc agctccttgc
     2701 cgacgccgct ttcgccgagg atcagcaccg ggatgttgga tttcgccgcg cgctcgccca
     2761 ggcggcgcac catctgcatg gcgggcgagg aaccgatcag gtcgtcgaac gaggcgcggt
     2821 tgtcgttgcg cttttgcagc cgctgcacct cggcggacag atcgcccatc tgcagcgcgt
     2881 tgcgcaccga gacgaggatg cgctccggcg aggcgggctt gatgaagaag tcctgcgccc
     2941 cggcctgcat cgccttgacc acggtgtcga tgccgcccga cgcggtcagc accaccaccg
     3001 gcgtgcgcac gcccgccgcg cggatgtcct tcaaggtgtc gatgcccgac tggcccggca
     3061 tgaccagatc gaggatgacc gcgtcgatgc cggagttctg cgtcagccgc tcataggcct
     3121 gcgccccgtt ctcggcgtgc atgaccgaaa agccctcgcg ctccagcacg gcctggatca
     3181 ggcggcgctg cgtcgggtcg tcgtcacaga tcaggatcgt cttggccata tcgaaatcac
     3241 caccatcgtt ctgatgcggc ctcgcctcat ggacacggcc tgttttggga catgtgtatc
     3301 gtcttggggt aaaaatcggg tgtagctcta tccttaacgc atcc
//
LOCUS       DASVLL010000215         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_387869,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000215 DASVLL010000000
VERSION     DASVLL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387869"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VF402_03510"
     CDS             <1..321
                     /locus_tag="VF402_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HEX7799389.1"
                     /translation="GGYQLALSLDIFRGRYVHGFATPGPLTPGKVTEYAWSLPNVDHV
                     FLPGHRIMIQVQSTLFPLYDRNPQTWVDNIFNARPGDYRKATQSVFYGGGARSAVFLP
                     VVKD"
     gene            complement(325..1062)
                     /locus_tag="VF402_03515"
     CDS             complement(325..1062)
                     /locus_tag="VF402_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX7799390.1"
                     /translation="MSRATRLLDLLQLLRRHRYPVAGAELARELEISLRTLYRDIAAL
                     QAQGAHIEGEPGVGYVLRPGYMLPPLMLSPEELEALVLGARWVSDRGDARLGAAAQNA
                     LTKIVAVLPKDLADELDSTGLMVVPSWSAPRVQVDLGDVRAAIRAESRVNVRYVDLKE
                     QASERVVWPIALGFFEHVRILVVWCELRQAFRHLRADRIVAWEPLAGRYPKRRAALMK
                     AWKIEREEENARYEAEYPRPGEMATVQ"
     gene            1195..1812
                     /locus_tag="VF402_03520"
     CDS             1195..1812
                     /locus_tag="VF402_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX7799391.1"
                     /translation="MKHERLSRNPARTRDHLVAAAFAEMYRCGYRGADLNSILGRAGV
                     TKGALYHHFDSKDGLGHAVIEQAIAAITTGKWLAPLDDVDDPVDALKAIVAGTRLDPE
                     AVNGGCPLNNLAQEMSPLDEGFRLRLAQIFADWIGGVADALRLGQINGKVRRDLNARD
                     AATFFIAAYEGYISLAKTAQSADLLASGLRQLDTWLEGLRACPKP"
     gene            1797..>2519
                     /locus_tag="VF402_03525"
     CDS             1797..>2519
                     /locus_tag="VF402_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7799392.1"
                     /translation="MPETLNPIVSREAWLEARTALLAQEKALTRRLDALRAERMKLPW
                     VKVDKLYIFDTPGGPKTLADLFAGRSQLLVQHFMFAPDWDEGCVGCSLGADHIDGVLP
                     HLENHDVKFTAVSRAPLGKLEAFRRRMGWRFDWISSGDTDFNADYGVAFRDGAGVYNY
                     RAGQDLPDDLPGLSAFFRDADGTVYHTYSCYARGTEVAGGVFGLLDICPKGRNEAGNM
                     TGWLRHHDRYDGAAHACGCGSQG"
BASE COUNT          429 a          876 c          820 g          394 t
ORIGIN      
        1 ggcggctacc agctcgcgct cagcctcgac attttccgcg gccgctacgt gcacggcttc
       61 gccacgccgg gaccgctgac gcccggcaag gtcaccgagt acgcatggtc gctgccgaac
      121 gtcgatcacg tcttcctgcc cggccacagg atcatgatcc aggtgcagtc caccctgttc
      181 ccgctctacg accgcaatcc gcagacctgg gtcgacaaca tcttcaacgc caggcccggc
      241 gactaccgca aggcgacgca gagcgtcttt tacggcggcg gcgccagaag cgcggtcttc
      301 ctgccggtcg tgaaggattg acgattactg caccgtcgcc atctcgccgg ggcgcggata
      361 ttcggcttcg tagcgcgcgt tttcctcctc gcgctcgatc ttccacgcct tcatcagcgc
      421 cgcgcggcgc ttcgggtagc gtccggccag cggctcccac gccacgatgc ggtcggcgcg
      481 caggtggcgg aacgcctgcc gcagctcgca ccacacgacg aggatgcgca catgctcgaa
      541 gaagccgagc gcgatgggcc agaccacgcg ctcgctggcc tgctccttca ggtcgacata
      601 gcgcacattg acccggcttt cggcgcggat cgcggcgcgc acgtcgccga gatcgacctg
      661 cacgcgcggc gccgaccaac tgggcacgac catcaggccg gtggaatcga gttcgtcggc
      721 gaggtctttc ggcagcacgg ccacgatctt ggtcagggcg ttctgcgccg ccgcgcccag
      781 ccgggcgtcg ccccggtccg acacccagcg cgcgcccagc accagcgcct cgagctcctc
      841 cggcgacagc atcagcggcg gcagcatata gccgggccgc agcacatagc cgacgcccgg
      901 ctcgccctcg atatgcgcgc cctgcgcctg caaggcggcg atgtcgcggt aaagggtgcg
      961 cagggaaatc tccagctccc gcgccagctc cgccccggcc accggatagc ggtggcggcg
     1021 cagaagctgc aacagatcga gcagacgcgt ggcgcgcgac atggcgaaaa cccctcttga
     1081 aaccgtagcg aagtctgcgc gcatcttaat gccagtttct gtcagcaaaa agcgccttct
     1141 gccgccattt tttgtcactt gacggcggga taacaaagtt acataccgtt tggtatgaag
     1201 cacgaacgcc tctcccgcaa tcccgcccgc acgcgcgatc atctggtcgc cgccgccttc
     1261 gccgagatgt accggtgcgg ttatcgcggc gccgatctca acagcattct cggccgcgcg
     1321 ggcgtgacca agggcgcgct gtatcatcat ttcgacagca aggacggcct cggccacgcg
     1381 gtgatcgagc aggccatagc cgccatcacc accggcaagt ggctggcgcc gctcgacgac
     1441 gtcgacgatc cggtcgacgc gctcaaggcc atcgtggccg ggacgcggct cgacccggag
     1501 gcggtcaacg gcggctgccc gctcaacaat ctggcgcagg aaatgtcgcc gctggacgaa
     1561 gggttccgcc tgcggctggc gcagatcttc gccgactgga tcggcggcgt ggccgacgcg
     1621 ctgcggctgg gccagatcaa cggcaaggtg cgccgcgacc tgaacgcgcg cgacgccgcg
     1681 accttcttca tcgcggccta tgagggctac atctcgctgg ccaagaccgc gcaaagcgcc
     1741 gacctgctgg cgtcgggcct gcgccaactc gacacatggc tggaaggatt aagagcatgc
     1801 ccgaaaccct gaacccaatc gtatcgcgcg aagcgtggct cgaggcgcgc acggcgctgc
     1861 tggcgcagga aaaggcgctg acccggcgac tggacgcgct gcgcgccgag cggatgaaac
     1921 tgccgtgggt gaaggtggac aagctctaca tcttcgatac gccgggcggg ccgaagaccc
     1981 tggccgacct gttcgcgggc cgcagccagc ttctggtgca gcatttcatg ttcgcgcccg
     2041 actgggacga aggctgcgtc ggctgctcgc tgggcgcgga tcatatcgac ggcgtgctgc
     2101 cgcatctgga aaaccacgat gtgaagttca ccgccgtatc gcgcgcgccg ctgggcaagc
     2161 tggaggcgtt caggcggcgc atgggctggc gcttcgactg gatttccagc ggggacaccg
     2221 atttcaacgc cgattacggc gtggcgttcc gcgacggcgc gggcgtctat aattaccgcg
     2281 cgggccaaga cctgcccgac gacctgcccg gcctgagcgc ctttttcagg gacgcggacg
     2341 ggacggtcta tcacacctat tcctgctacg cgcgcggcac ggaggtcgcg ggcggcgtgt
     2401 tcggcctgct cgacatctgc ccgaaagggc gcaacgaggc cgggaacatg accggctggc
     2461 tgcgccacca cgaccgttac gacggcgcgg cgcacgcctg cggctgcggt tctcaaggc
//
LOCUS       DASVLL010000216         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_120220,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000216 DASVLL010000000
VERSION     DASVLL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120220"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VF402_03530"
     CDS             complement(<1..142)
                     /locus_tag="VF402_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEX7799393.1"
                     /translation="MSQTRILVFGNEKGGAGKSTVAMHVGVHLLHQGHRVAFLDLDLR
                     QRS"
     gene            342..515
                     /locus_tag="VF402_03535"
     CDS             342..515
                     /locus_tag="VF402_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799394.1"
                     /translation="MDPAAERMKCVMEAIDHGLTVNETLEECPHCAPQPDAIGLHQER
                     WDFWAPTSFSETL"
     gene            complement(528..926)
                     /locus_tag="VF402_03540"
     CDS             complement(528..926)
                     /locus_tag="VF402_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799395.1"
                     /translation="MLRAFADRMLLKITAMDDPEDMPGVERAVRTAAVIERLYSRCDR
                     AERCVDDPRKRRAERARHADAAVKAQASLAHTLKWSDKTRRSLGKWWDAAEKAAEAPQ
                     APTATRAACAIAAPDLKPSRTQSDETPLPP"
     gene            1136..1954
                     /gene="map"
                     /locus_tag="VF402_03545"
     CDS             1136..1954
                     /gene="map"
                     /locus_tag="VF402_03545"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7799396.1"
                     /translation="MTTDSDYMTRSDTIRIHTDADFEGMRKAGRIAADCLDMLIPYVQ
                     PGVATQALDDLAREFIFDHGALPACVFYRGYQNTVCISPNHVVCHGIPSEKSLRDGDI
                     INIDVTCVADGWHGDTSRMYEVGQVPAKAKRLIDVTYEAMRLGLEQIRPGNTFGDIAA
                     AIQRYAEAQRCSVVRDFCGHGVGRVFHDTPNVLHFGKAGTGPVLQKGMFFTVEPMINL
                     GRYEVKVLNDGWTAVTRDKTLSAQCEHSCGVTDDGVELFTASRTGQFRPTPVAG"
BASE COUNT          336 a          641 c          715 g          390 t
ORIGIN      
        1 gcgatctttg ccgcaggtcg agatcgagaa aggcgacccg gtggccctga tgcagcagat
       61 gcacgccgac gtgcatggcg acggtcgact tgcccgcccc gcccttttcg ttgccgaaca
      121 ccagaatgcg cgtttgcgac atgaactccc actcccgccg actcaaccgt ccaccagact
      181 taagcgctct tgccgtccgc cgccagagcg cgtcaatctt tggttaacat attgtcgtgg
      241 gtaaatggcg tgcggatgtt cgcacgtgca aacaaatgtg attttccaac ccattcgatc
      301 gagcgcacac tcggcctgtt gctcataagg gagatccggc gatggatcct gccgcggaac
      361 ggatgaagtg cgtgatggag gccatcgatc acggcctcac ggtcaacgag acgctggagg
      421 aatgtcctca ttgtgcgccg caaccggacg ccatcggcct gcatcaggaa cgctgggact
      481 tctgggcgcc gacatcgttt tccgaaacgc tgtaagcgcc tgtccgccta gggcgggagg
      541 ggcgtttcat cgctttgcgt gcgggacggt ttcaggtccg gcgccgcgat ggcgcaggcg
      601 gcgcgcgttg cggtcggcgc ttgcggagct tccgccgcct tttcggcggc gtcccaccat
      661 ttgccgaggc tgcggcgggt cttatcgctc catttcaggg tgtgggccag ggacgcctgc
      721 gccttgaccg ccgcgtccgc atgccgggcg cgttcggcgc gccgtttgcg cggatcgtcc
      781 acgcagcgtt cggcgcggtc gcagcggctg tagaggcgtt cgatgacggc ggctgtgcgc
      841 acggcgcgtt cgacgccggg catgtcttcg gggtcgtcca tcgccgtgat tttcagcagc
      901 atccggtcgg cgaaggcgcg tagcatctcg cggcgttctt ccggggtcag ggggtgctga
      961 ggcgggcgtg ggtccgaggg catggggaca ggatgcgccg ggaaatcggg gcggggatag
     1021 atttttgctg gaggccgcct cccacccttg ccttgggcgg gcgcgtggtt ggcttttacg
     1081 ggcggggggc gtcgctgctc tggatatatg ccaggcttgc ggctatagtg gccggatgac
     1141 cacagattcc gattatatga cccgttccga cacgatccgt attcatacgg acgcggattt
     1201 cgagggtatg cgcaaggcgg gccgcatcgc ggcggattgc ctcgacatgc tcatacccta
     1261 tgtgcagccg ggcgtggcga cgcaggcgct cgacgatctg gcgcgcgaat tcatcttcga
     1321 tcacggcgcc ctgcccgcct gcgtgtttta ccgcggctat cagaacacgg tctgcatctc
     1381 gcccaaccat gtggtctgtc acggcattcc gtcggaaaaa agcttacgtg acggcgatat
     1441 catcaatatc gacgtcacct gcgtggccga cggctggcac ggcgacacct cgcgcatgta
     1501 cgaggtcgga caggttccgg ccaaggcgaa gcgcctgatc gacgtgacct atgaggcgat
     1561 gcgcctcggc ctcgaacaga tccggccggg aaacaccttc ggcgacatcg cggcggccat
     1621 tcagcgctat gcggaggcgc agcgctgctc ggtggtgcgc gacttctgcg gccacggcgt
     1681 cgggcgcgtg ttccacgaca cgcccaacgt gctgcatttc ggcaaggccg ggaccgggcc
     1741 ggtgctgcaa aagggcatgt tcttcaccgt cgagccgatg atcaatctcg gccgctacga
     1801 ggtgaaggtg ctgaacgacg gctggaccgc cgtgacgcgc gacaagacgc tgagcgcgca
     1861 gtgcgaacat tcgtgcggcg tgaccgacga cggcgtggag ctgttcaccg cctcgcgcac
     1921 gggccagttc cggccaacgc cggtcgcggg ataagtatcg tctggggggg acggatgggg
     1981 gcggacgccg caagcgatag tggacttccc cagcatggcg ggcatcgcca gcgcctgcgc
     2041 gaccgcgcgc gcaagggcgg cgtgtccgtg ctgcccgact at
//
LOCUS       DASVLL010000217         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_443403,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000217 DASVLL010000000
VERSION     DASVLL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443403"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF402_03550"
     CDS             <1..381
                     /locus_tag="VF402_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX7799397.1"
                     /translation="AARAQEPPPRPDFAALDANHDGRLDLSEFTAPMLARAKAMFDRM
                     DANHDGVVTESEFNAFRPEGGPPRDGPPRMGPPPPDFKRLDANGDGKVSFAEFSAREK
                     ARFDRLDANHDGVLEPAELSPPQN"
     gene            complement(597..>2212)
                     /locus_tag="VF402_03555"
     CDS             complement(597..>2212)
                     /locus_tag="VF402_03555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX7799398.1"
                     /translation="MGLIFFPPPLLKALVKSQFFTTLYLSNFAFARESGYFDLQAELK
                     PLLHTWSLAVEEQFYLIYPPVLYALYRWARPLMWPVLALLALASLYVCQINLERYPAP
                     AFYWPISRAMELLIGALAAGVPPHLKLNARAQSLLGGAGLAAILAGYVLIDDETPFPG
                     LWALLPCLGTAALLLAPQGLPGRLLSVKPLVRVGDVSYSLYLWHWPLLVFARFLFPDA
                     VWPVIAALVLSAGLSWLSWRYIEAPFLRRPDKPIRWFALLCMAASIAACLLIFYNGSL
                     PQRFSPEARAYLAASNDYNHDRKTCHMESDKPIPYAQTCVYGDRNAAPAYAVWGDSHG
                     AELSRALGRKLGAYGQSLRELTMSGCPATLSRKPVCRQHNLDMMAAIKADPNMQTVIL
                     VGNLHDDDPSARAATAGMEQTALDLQKAGKTAIIVYPIPIYRFDPPSQLALSSRARRP
                     ATAVGQPVSEYLARHQAIIDEFDRFTARNGIQHVEPQSLFCDDDLCHVYQPGVGVLYF
                     NAGHLSLTGAGVLADRILSQLALSDQPEK"
BASE COUNT          409 a          739 c          753 g          311 t
ORIGIN      
        1 gcggcgcggg cgcaggagcc gccgccgcgc cccgacttcg cggcgctgga cgccaatcac
       61 gacggcaggc tggacctgtc cgaattcacc gcgccgatgc tggcgcgcgc caaggccatg
      121 ttcgacagga tggacgccaa ccacgacggc gtcgtcaccg agagcgaatt caacgccttc
      181 cgccccgaag gcggtcctcc ccgcgacggc ccgccccgca tgggaccgcc gccgccggac
      241 ttcaagcgcc tcgacgccaa cggcgacggc aaggtcagct tcgcggaatt ctcggcgcgc
      301 gaaaaagccc gcttcgaccg cctggacgcc aatcacgacg gcgtgctgga gcccgccgaa
      361 ctgtccccgc cgcaaaactg acgcccgaat tcgccctccc cgttcacgcg gtgggagggg
      421 caagccggtc ccgaaaattt tttccagccg ccgcgacgga attttcctga acgagcgttc
      481 aacttcgcga acgtcgccct tccggacggc gtaagaggcc gtgcccgccg gtatctcccc
      541 ggagccgtcg cggaaacgga agagccgtga cagccgccgc tgccacggct ctttttttat
      601 ttctccggct gatcactcag cgccagttgc gacaggatcc ggtcggccag cacgcccgcg
      661 ccggtcaggc tcagatggcc cgcattgaag tacagcaccc cgacgccggg ctgatagacg
      721 tggcacagat cgtcgtcgca gaacaggctc tgcggctcga catgctggat gccgtttcgc
      781 gccgtgaagc ggtcgaactc gtcgatgatc gcctgatggc gcgccagata ttccgacacc
      841 ggctggccga ccgccgtggc gggccgccgc gcgcggctgg acagggccag ttgcgacggc
      901 gggtcgaacc tgtagatcgg gatcggatag acgatgatgg ccgtcttgcc cgccttttgc
      961 aggtcgagcg cggtctgctc catgcccgcc gtcgccgcgc gcgccgaagg atcgtcgtcg
     1021 tgcagattgc cgaccagaat cacggtctgc atgttcggat cggccttgat cgcggccatc
     1081 atgtcgagat tgtgctgacg gcagaccggc ttgcggctca gcgtggccgg acagcccgac
     1141 atggtcagct cgcgcaggga ttgcccgtag gcgcccagct tccggcccag cgcgcgcgac
     1201 aactcggcgc cgtggctgtc gccccagacc gcataggcgg gcgcggcgtt tctgtcgcca
     1261 tagacgcagg tctgcgcgta ggggatcggc ttgtccgact ccatgtggca ggtcttgcgg
     1321 tcgtggttgt agtcgttcga cgcggccaga taggcgcgcg cctcgggcga aaagcgctgc
     1381 ggcagactgc cattgtagaa gatcagcagg caggcggcga tggaggcggc catgcagagg
     1441 agcgcgaacc accggatcgg cttgtccggg cggcgcagga acggcgcctc gatatagcgc
     1501 cacgacagcc agctcagccc ggcggacagg accagcgccg cgatgaccgg ccagaccgcg
     1561 tcgggaaaca ggaagcgcgc gaacaccagc agcggccagt gccacaggta gagcgaatag
     1621 gagacgtcgc cgacgcggac cagcggcttt acgctcagca ggcggcccgg caggccctgc
     1681 ggcgccagca gcagcgcggc ggttccgagg cagggcaaaa gcgcccaaag gccgggaaac
     1741 ggcgtctcgt cgtcgatcag cacgtaaccg gcgagaatgg cggccagacc cgcgccgccc
     1801 agcaggcttt gcgcgcgggc gttcagtttc aggtggggcg ggacgcccgc cgccagcgcg
     1861 ccgatcagca gttccatggc gcgcgagatc ggccagtaga aggccggggc cgggtaacgc
     1921 tccagattga tctggcagac atagagcgag gccagcgcca gcagggccag aaccggccac
     1981 atcagcgggc gcgcccagcg ataaagcgca tacaggaccg gcggatagat caggtagaac
     2041 tgctcctcga ccgccagcga ccaggtgtgc agaagcggct tcaactcggc ctgcaagtcg
     2101 aaatagccgc tctcgcgcgc gaaggcgaag ttggacagat aaagcgtggt gaagaactgg
     2161 ctcttgacca gcgccttgag cagcggcggc gggaaaaaga tcagccccat ga
//
LOCUS       DASVLL010000218         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_66378, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000218 DASVLL010000000
VERSION     DASVLL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66378"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VF402_03560"
     CDS             <1..359
                     /locus_tag="VF402_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7799399.1"
                     /translation="FVRIRDHGDGVPTDMQAAVFDKYMRLQKADQQNAGTGLGLTICQ
                     SIMRLLDGRIELDNHPDGGAVFTLYYPDKKRAYDGPAHEKSRPGRERLFYRPFLSSVR
                     APDQTGGLALRPVIMR"
     gene            complement(317..499)
                     /locus_tag="VF402_03565"
     CDS             complement(317..499)
                     /locus_tag="VF402_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX7799400.1"
                     /translation="MLNWIITFLILAVIAAFFGFGGLAGTFAGIAKFIAIVFVVLFIA
                     SLVYRMITGRSANPPV"
     gene            complement(621..1316)
                     /locus_tag="VF402_03570"
     CDS             complement(621..1316)
                     /locus_tag="VF402_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7799401.1"
                     /translation="MLEKKNTILVVDDEEEIRKMLGIFLDAADFKVCECDSGKQALRM
                     SASVRPDLILLDLGLPDIDGKDVIAKVREWSNVPIVVLTARAEDMDAAPALNAGADDY
                     VTKPFSAEVLLARINANLRKSAVREVGEPEIVHGPIRMDLVRHEVFIDDEKVSFTPKE
                     YDLLRFFLVNRGRMLTHKQILKEVWGPAHLEDTQYLRVYIGQVREKLEARPGLGKSIV
                     SESGIGYRMDQVA"
     gene            complement(1406..1705)
                     /locus_tag="VF402_03575"
     CDS             complement(1406..1705)
                     /locus_tag="VF402_03575"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX7799402.1"
                     /translation="MQKLSADMAAQSLADLDGWTLAPDAGAMTRDFRFPDFKAAFAFM
                     SEVAAEAERMDHHPEWSNVYNRVSVRLTTHDAGGLTGRDVRLARFIDAAARRQAL"
     gene            1849..>2993
                     /locus_tag="VF402_03580"
     CDS             1849..>2993
                     /locus_tag="VF402_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEX7799403.1"
                     /translation="MKFATASVLAALWLASGPALAGPAGGAASAAYKDIVPKLVMVDA
                     DLRGGLNLDGAWRYSVDPYKDGLADFLGKSTDVRHGRGADIVVADVEKANPNALFEYD
                     LGNAPLGEVPSSWIGYDPTLRYYNGLMWYQKRFDAGPDLIRKDGRVFLHFDAADYHAM
                     IYLNGHKVGEHFGGFTPFTFEVTDIVRAKDNNLVVGVDSTRTADTIPPTVTDWETYGG
                     ITRDVKLIATPATYIDDAFIRLTKDGRIAADMALDGAGKAGQAVEIAVAGTPLVLKGT
                     TDAEGRVSMSAAAPKGLKLWSPDTPTLYDVTFKAGADAITDRIGFRTIEVQGAKILLN
                     GKPIFLRGVAMHEEEFGPNPSRRMTEEASRALLTEIKDGLHGNYVRLA"
BASE COUNT          570 a          956 c          936 g          531 t
ORIGIN      
        1 gcttcgtgcg catccgcgat cacggcgacg gcgttccgac cgacatgcag gccgccgtgt
       61 tcgacaaata catgcgcctg cagaaggccg accagcagaa cgccggcacg ggcctcggcc
      121 tgaccatctg ccagtcgatc atgcggctgc tggacggccg catcgagctg gacaaccacc
      181 cggacggcgg cgcggtcttc accctctact accccgacaa gaagcgcgcg tatgacggac
      241 cggcccacga aaaaagccgc cccggaaggg agcggctttt ttatcgtccg tttttatcgt
      301 ccgtgcgggc gccggatcag accggcggat tggcgctgcg gcccgtgatc atgcgataga
      361 cgaggctggc gatgaacagc accacgaaga cgatcgcgat gaacttggcg atgccggcga
      421 aggttccggc cagtccgcca aagccgaaaa aggccgcgat gaccgccaga ataagaaagg
      481 tgatgatcca gttaagcatg agattctcca aaacctgaga cttatcatca gattaggagg
      541 accgcggtaa ggaagcaaag ctgtcgccga gcgccgccgt aagaaaaacg taagcgcggc
      601 gcttacgctt cttgccgccc ctacgccacc tgatccatgc ggtagccgat gccggattcc
      661 gacacgatcg acttgcccag acccggacgg gcctccagct tttcgcgcac ctgaccgata
      721 tagacgcgca aatattgcgt gtcctcgagg tgcgccggac cccagacctc tttgagaatc
      781 tgcttgtgcg tcagcatgcg gccgcgattg accaggaaga agcgcagcag gtcgtattcc
      841 ttgggcgtga agctgacctt ctcgtcgtcg ataaagacct cgtgacggac cagatccatg
      901 cggatcgggc catgcacgat ctccggttcg ccgacctcgc gcaccgccga cttacgcagg
      961 ttggcgttga tgcgcgccag cagcacctcg gcggaaaacg gcttggtgac gtagtcgtcg
     1021 gcgccggcgt tgagcgcggg cgcggcgtcc atgtcctcgg cgcgcgcggt cagcaccacg
     1081 atcggcacgt tcgaccattc gcgcaccttg gcgatcacgt ccttgccgtc gatgtcgggc
     1141 aggccgaggt cgagcaggat caggtccggg cgcaccgaag cgctcatgcg caacgcctgc
     1201 ttgcccgaat cgcattcgca gaccttgaaa tcggcggcgt cgagaaaaat gcccagcatc
     1261 ttgcggattt cttcttcgtc atccaccacc aggatcgtat tcttcttttc gagcatcgca
     1321 ggttccgtat gttgattacg tgctacgggt gtaggttcag aaaaataaaa atgaaatgcg
     1381 gtagtcggac gcctgttttg gcgccttaca gggcctgcct tcgcgccgcc gcgtcgatga
     1441 agcgcgccag ccgcacgtcg cgcccggtca gccccccggc gtcgtgcgtg gtcagccgca
     1501 ccgacacccg gttatagacg ttcgaccatt cgggatggtg gtccatgcgc tcggcctcgg
     1561 cggccacctc gctcatgaag gcgaaggccg ccttgaaatc cggaaagcgg aaatcgcgcg
     1621 tcatcgcccc ggcgtccggc gccagggtcc agccgtccag atcggcgaga gattgcgcgg
     1681 ccatatcggc ggatagcttt tgcattccgc gtcctcctct gcgaaattat aaatccgggg
     1741 aaggtttgac cgcaagcccg acgctttagg gcttgaaatt tgctccggcc tgaccgattg
     1801 gtcagaccgg tttagacaac aattcatgat ctgccggagg aaaccctgat gaaattcgca
     1861 accgcatccg ttctggcggc gctttggctc gcctccggtc ccgccctggc cggtccggcg
     1921 ggcggcgccg cttcggccgc ctataaggac atcgtgccga agctcgtcat ggtcgacgcc
     1981 gacctgcgcg gcggcctcaa tctggacggc gcctggcgtt attcggtcga tccgtacaag
     2041 gacgggctgg ccgacttcct cggcaagtcg accgatgtgc gccacggacg cggcgccgac
     2101 atcgtggtcg cggacgtgga gaaggccaat cccaacgccc tgttcgagta cgacctcggc
     2161 aacgcgccgc tgggcgaggt gccgtcgtcg tggatcggct acgatcccac cttgcgctat
     2221 tacaacggcc tgatgtggta tcagaagcgc ttcgacgccg ggccggatct gatccgcaag
     2281 gacgggcgcg tgttcctgca tttcgacgcc gccgattatc acgccatgat ctatctcaac
     2341 ggtcacaagg tgggcgagca tttcggcggc ttcacgccgt tcaccttcga ggtcacggac
     2401 atcgtccgcg cgaaggacaa caatctcgtg gtcggcgtcg attcgacgcg caccgccgac
     2461 acgattccgc cgaccgtgac cgactgggag acctatggcg gcattacgcg cgacgtgaag
     2521 ctgattgcca cccccgccac ctatatcgac gacgccttca tccgcctgac gaaggacggc
     2581 cgaattgcgg ccgacatggc gctggacggc gcgggcaagg ccggtcaggc ggtcgagatc
     2641 gccgtcgccg ggacgccgct ggttctgaaa gggaccacag acgccgaggg ccgcgtctcg
     2701 atgagcgcgg cggcgccgaa agggctgaaa ctgtggtcgc cggatacgcc gacgctgtac
     2761 gacgtgactt tcaaggcggg cgcggacgcg atcacggatc ggatcggctt tcgcaccatc
     2821 gaggtccagg gcgcgaaaat cctgctcaac ggcaagccga tcttcctgcg cggcgtcgcc
     2881 atgcacgagg aggagttcgg tcccaacccg tcgcgccgca tgaccgagga ggcttcgcgc
     2941 gcgctgctga ccgagatcaa ggacgggctg cacggcaact atgtgcggct ggc
//
LOCUS       DASVLL010000219         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_497517,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000219 DASVLL010000000
VERSION     DASVLL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497517"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VF402_03585"
     CDS             <1..1009
                     /locus_tag="VF402_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7799404.1"
                     /translation="KDKSFPDLPPQWRSRPQYVYTQGPHVHDYLQEMRREVLDAYDTM
                     NVGEAFGVTLARTPDFIDERRGELDMAFQFAAVEVDRDENGHWKPWSLPELKAVFARQ
                     DAAFDTSCWPAVFLSNHDNPRLVSRYGDDSPAWRVKSAQVLATLLLTQEGTPFLYQGD
                     EIGLTNLRFTSPDQFNDVWSKNGWRDQVESGRMRAGDFLAEQNHLSRDHARAPMPWNG
                     GCNGGFTTAPTPWFALHPDFGVINAAGSGDIYRYYTRMIALRRKHLALIYGDYNDLAP
                     EHPRLFAYERRTDGERLRTYLNMSAQPLDTSLPDGDCILCNYGAPAPMLRGWEARIYR
                     L"
     gene            1031..>1502
                     /locus_tag="VF402_03590"
     CDS             1031..>1502
                     /locus_tag="VF402_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HEX7799405.1"
                     /translation="MTDPVLETLMLPFHDGALDWPDAPALFLRARPVEAPRDRLICEQ
                     SFKPMHAALTRAGFVLRGDQAGPFPMVLVLPPRQREEYRALLARAVDETAAGGVVVAS
                     ASNLEGARTIESDLKALCGGVASLSKNKCRVFWAQVDPARLNRDLLAQWAALDAP"
BASE COUNT          251 a          478 c          500 g          273 t
ORIGIN      
        1 caaggacaaa agctttcccg acctgccgcc gcaatggcgt tcacgcccgc aatacgtcta
       61 tacgcagggg ccgcacgtgc atgactattt gcaggagatg cggcgcgagg ttctggacgc
      121 ctacgacacg atgaatgtcg gcgaggcgtt cggcgtgacg ctggcgcgga cgccggactt
      181 catcgatgag cggcgcggcg agttggacat ggcgtttcag ttcgccgccg tcgaggtcga
      241 ccgcgacgaa aacggccatt ggaagccgtg gagcctgccc gaattgaagg cggtgttcgc
      301 gcgtcaggac gcggcgttcg atacctcctg ctggcccgcc gtctttttgt ccaaccacga
      361 caatccgcgc ctcgtctcgc gctatggcga cgacagcccg gcgtggcgcg tgaagtccgc
      421 gcaggttctg gccacgctgc tgctgacgca ggagggaacg cccttcctct atcagggcga
      481 cgagatcggg ctgaccaatc tgcgcttcac ctcgccggat cagttcaacg atgtctggtc
      541 aaagaacggc tggcgcgatc aggtcgaaag cgggcgcatg agggccgggg atttcctcgc
      601 cgaacagaac catctgagcc gcgatcacgc ccgcgcgccg atgccgtgga acggaggttg
      661 caacggcgga ttcaccacgg cgccgacgcc gtggttcgcg ctgcaccccg atttcggcgt
      721 tataaacgcg gctggttcgg gggatattta tcgttattac acacgtatga tcgccttgcg
      781 tcgtaagcac ttggcgctga tctacggcga ttataacgat ctggcgccgg agcatccgcg
      841 cctgttcgcc tatgagcgcc gcactgacgg cgaacggttg cggacctatc tcaacatgtc
      901 ggcgcaaccg ctcgatacgt ccttgccgga cggcgattgc atcctgtgta attacggcgc
      961 accggcgccg atgctgcgcg gctgggaggc gcggatttac aggctgtgac gcctgtgcga
     1021 taggcaggcc atgaccgatc ccgtgcttga aaccctgatg ctgccgttcc atgacggcgc
     1081 gctcgactgg ccggacgcgc ctgccctgtt cctgcgcgcg cgacccgtcg aggcgccgcg
     1141 cgaccggctg atctgcgagc agagttttaa gccgatgcac gctgcgctga cgcgcgcggg
     1201 cttcgtcctg cgcggcgatc aggccgggcc gttcccgatg gtcctcgtgc tgccgccacg
     1261 tcagcgtgag gaatatcgcg ccctgctggc gcgcgccgtc gatgagacgg cggcgggcgg
     1321 cgtcgtggtc gccagcgcgt ccaatcttga aggcgccagg accatcgaaa gcgacctgaa
     1381 ggcgctgtgc ggtggcgtcg ccagcctgtc gaaaaacaaa tgccgcgtct tttgggcgca
     1441 ggtcgatccg gcgcggctga atcgcgatct gctcgcgcaa tgggccgcgc tcgacgcgcc
     1501 gc
//
LOCUS       DASVLL010000220         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_66432, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000220 DASVLL010000000
VERSION     DASVLL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66432"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..864)
                     /locus_tag="VF402_03595"
     CDS             complement(235..864)
                     /locus_tag="VF402_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX7799406.1"
                     /translation="MSDKSVYLAALAACVLLAACHKAPPKEAAAPAVPPPPPPPPLQP
                     LPARAPGLWETSLTEIGSAEAPQTLQICIDAQVDKRLGILGNDLSGEACTEKGYRPQG
                     DGSLGFLAECHTGAGVVTEYSGSIDGDYTHDYTMKARLQTTGANLNRVASYEVVSRRV
                     GACKPDQKPGDMISDGVKVNLVDMAGQGTGQGKDKGKSDGDKMSAAAPD"
     gene            complement(951..1334)
                     /locus_tag="VF402_03600"
     CDS             complement(951..1334)
                     /locus_tag="VF402_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799407.1"
                     /translation="MVPPASKEVALGQVIDGDKVMSLPTQVTHKADIEVAEGPVVRES
                     RTQRVRRLQEEARELAAEQISEFETLLDATARMALEIADGGEVYAIGAREICRRLAEE
                     LPRTLQTLQVITKKTTNFAEAVKRG"
     gene            complement(1638..>2378)
                     /locus_tag="VF402_03605"
     CDS             complement(1638..>2378)
                     /locus_tag="VF402_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7799408.1"
                     /translation="NQVDDVIAEVRKVAQAGYRSLVTVLTKKMAESLTDYMHEQGVRV
                     RYMHSDIDTLERIEIIRDLRLGAFDVLVGINLLREGLDIPECGFVAILDADKEGFLRS
                     ETSLIQTIGRAARNVDGRVILYADRVTGSMERALAETTRRREKQLAYNEAHGITPESV
                     KRGIADILDSPYEKGDRYQAPMGVAEKDAKPFLGSNFQTTLKDLEARMREAAGNLEFE
                     EAARLRDEIKRLKTLDLAFAKDMLDSDG"
BASE COUNT          377 a          768 c          755 g          478 t
ORIGIN      
        1 ccttcgtgcg caggcacggc gcgacgcccg aactgaacat cgccatgctg ggactggtgc
       61 tgctctgcct cggctatacg gtcgcgcggg cggtcatggg cttcaaccac ctgctctatt
      121 tccgcccgtt gcccaatccg aagacgtggc tggtctagcc tgatcggggc tctaagtctt
      181 tgttttgtcg ctcttccgta tccaaaaagt ggagccactt tttggggaaa cgctctaatc
      241 cggggcggcc gccgacatct tgtccccgtc gcttttgccc ttatccttac cttgcccagt
      301 tccctggcca gccatatcga cgaggttgac cttgacgccg tcgctgatca tgtcgccggg
      361 cttctggtcc ggcttgcagg cgccgacgcg gcgggagacg acctcgtaac tggccacgcg
      421 gttcaggttg gcgcccgtgg tctgcaaccg cgccttcatg gtgtaatcat gcgtatagtc
      481 gccgtcgatc gagccggaat attccgtcac cacgcccgcg ccggtgtggc attcggccag
      541 aaagccgagg ctgccgtcgc cttgcggccg atagcccttc tctgtgcagg cctcgccgct
      601 cagatcgttg ccgagtatgc cgaggcgctt atccacctgc gcgtcgatgc agatttgcag
      661 cgtctgcggc gcctcggcgc tgccgatttc ggtcaggctc gtctcccaca ggcccggcgc
      721 gcgcgcgggc agaggctgca agggcggcgg cggaggcggg ggcggaacgg cgggcgcggc
      781 ggcctccttc ggcggcgcct tatggcaagc cgccaacagg acgcaggccg cgagggccgc
      841 caggtaaacc gacttgtccg acatgctcgc ctgtttccgc tgctgacagg cggcaaggct
      901 aagggaaacg gaccgcgagc gccagcgata aagttcgcgc cagcggcgaa tcagccgcgc
      961 ttcacggctt cggcgaaatt ggtggtcttt ttggtgatca cctgcaaggt ttgcagcgtg
     1021 cgcggcagct cctcggccag acggcggcag atttcgcgcg cgccgatggc gtacacctcg
     1081 ccgccgtcgg cgatttccag cgccatccgg gccgtggcgt cgagcagggt ctcgaattcg
     1141 ctgatctgtt cggcggccag ttcgcgggct tcttcctgaa gacggcggac gcgctgggtg
     1201 cggctttcgc gcacgacagg cccttccgcg acctcgatgt cggccttgtg agtcacctgc
     1261 gtcggcaggc tcatcacctt gtcgccgtcg atgacctgcc ccagcgccac ttccttggaa
     1321 gcgggcggca ccacattcaa tcgagtccta cgcatcttca aaaccccgct gcgaaacacc
     1381 ggtccgtaca cgctgggccg tctgatggcg tgacgccact tccggtagag tctccggggg
     1441 gaaggttaag aagcgcttaa atcccccggc gtcgacgcga actgcggccc gatgctgagg
     1501 gtctcgcgtt tcaaaataaa aatccagagg aaatatggcg cgattacgtc aggaagcgcg
     1561 cttttatcca cagggtgcga caaaaacgac gctcctggcg cgccatatac gaggcttata
     1621 tgagactttt ccggatttca tccgtccgaa tcgagcatgt ccttggcaaa cgccaggtcc
     1681 agcgtcttga gtcgcttgat ctcgtcgcgc aggcgcgccg cctcctcaaa ctccagattg
     1741 cccgccgcct cgcgcatccg cgcttccaga tccttcagcg tcgtctggaa gttggagccg
     1801 aggaacggct tggcgtcctt ttcggccacg cccatcggcg cctgataacg gtcgcccttt
     1861 tcataggggc tgtcgagaat gtcggcgatg ccgcgcttga ccgattcggg cgtgatgccg
     1921 tgcgcttcgt tataggccag ttgcttctcg cggcggcgcg tggtttcggc cagggcgcgc
     1981 tccatcgagc cggtgacgcg gtcggcgtag aggatgacgc ggccgtcgac gttccgcgcg
     2041 gcgcggccga tggtctggat cagcgaggtt tccgagcgca ggaagccttc cttgtcggcg
     2101 tcgagaatgg cgacgaagcc gcattcggga atgtccagcc cttcgcgcag caggttgatg
     2161 ccgaccagca cgtcgaaggc gcccagccgc aggtcgcgga tgatctcgat gcgctccagc
     2221 gtgtcgatgt cggagtgcat gtagcgcacg cgcacgccct gctcgtgcat atagtcggtc
     2281 aggctctcgg ccatcttctt ggtcagcacg gtgaccagcg agcggtaacc ggcctgcgcc
     2341 accttgcgca cctcggcgat gacgtcatcg acctgatt
//
LOCUS       DASVLL010000221         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_443613,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000221 DASVLL010000000
VERSION     DASVLL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443613"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VF402_03610"
     CDS             <1..465
                     /locus_tag="VF402_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799409.1"
                     /translation="WGAALCLMGLTAGDGGDFSLSLNKAGSGHSAQILMQDNYVTRAI
                     IGLMGDDRLSFKVSPDGSSYITALSVGSDGAVATPAGTVIDAGGRLALPVCAVAALPV
                     SGVAAGTLAYASDARVFDGAGVLEAAGSGTGGLVCWSGSGWRLAGTHVAVSA"
     gene            462..1199
                     /locus_tag="VF402_03615"
     CDS             462..1199
                     /locus_tag="VF402_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEX7799410.1"
                     /translation="MKMLRSEAVRHWSTWLWGAALCLMGLTASLPGWLVQFLPGVLPV
                     LHGPARDRAVLILGVAGFLAKFVAQAAVRERIVRLRDWLKHMAASGGAVRAQHAAGAA
                     LAAALALAVPLIARWEGRANDPYLDMARIRTVCFGHTGGVEARRYGDAECEALLRKDA
                     LDHARPVLACSPRLAGCVGMLAAFVSFDFNTGAYCRSAIPALVAKGEDRAACAQLSRW
                     VYVRGRRVPGLVNRRAAERALCERGLS"
     gene            1211..1648
                     /locus_tag="VF402_03620"
     CDS             1211..1648
                     /locus_tag="VF402_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799411.1"
                     /translation="MGAAILLLLAAFGAGYLTRVFTARPQIKTEIRAAARAQKAETKL
                     IVIHDKVAQAAKEAHDGIAGAAAQARPGCDESGVVSAWRDGLERVRQAAGAAADSAGG
                     ASAVPLAGPAQRQDDAGSGRLLDRAGTRADDLRSRTQGADQGD"
BASE COUNT          233 a          568 c          695 g          318 t
ORIGIN      
        1 tggggcgcgg ccctgtgtct gatggggctg acggcgggcg acggcggcga tttcagcctg
       61 agcctgaaca aggcgggcag cggccacagc gcgcagattc tcatgcagga caattacgtc
      121 acccgcgcca ttatcggcct catgggcgac gaccgcctga gcttcaaggt gtcgccggac
      181 gggtccagct atatcacggc gctcagcgtc ggttccgacg gcgcggtggc gacgcccgcc
      241 gggacggtga tcgacgcggg cgggcggctg gccctgccgg tgtgcgcggt ggcggccctg
      301 ccggtttcgg gcgtggcggc ggggacgctg gcctacgcca gcgacgcgcg cgtcttcgac
      361 ggcgccggcg ttctggaggc ggcaggttcc ggcacgggcg gactggtgtg ctggagcggg
      421 tcgggctggc ggctggccgg cacccatgtg gcggtttcgg catgaaaatg ttgcgatcgg
      481 aggcggttcg gcactggtcg acatggctct ggggcgcggc cctgtgcctg atggggctga
      541 cggcgtcgct gccgggctgg ctggttcagt tcctgccggg cgtgctgccg gtgctgcacg
      601 gcccggcccg cgaccgggcg gtgctgatcc tcggcgtggc gggctttctg gccaagttcg
      661 tcgcgcaggc ggcggtgcgt gagcgcatcg tcaggctcag ggactggctg aaacacatgg
      721 ccgcgtccgg cggcgcggtc agggcgcagc acgcggcggg cgcggcgctg gcggcggcgc
      781 tggctttggc cgtcccgctg atcgcgcgct gggagggccg ggcgaacgat ccctatctcg
      841 acatggcgcg gattcgcacg gtctgtttcg gccacaccgg cggggtcgag gcgcggcgct
      901 acggcgacgc ggagtgcgag gccctgctgc gcaaggacgc gctggaccac gcgcgaccgg
      961 tgctggcctg ttcgccgcgg ctggccgggt gcgtcggcat gctggcggcg ttcgtcagct
     1021 tcgacttcaa caccggcgcc tattgccgct cggcgatccc ggccctggtg gccaaaggcg
     1081 aggaccgcgc cgcctgcgcg cagttgtcgc gctgggtcta tgtgcgcggg cggcgcgttc
     1141 cgggcctcgt caaccgccgc gccgccgagc gcgccttgtg cgagcggggg ctgtcatgac
     1201 ggtaagggat atgggcgcgg ccatcctgct gctgctggcg gcgttcggcg cgggctatct
     1261 gacgcgcgtt ttcacggcgc gtccgcagat caaaacggag attcgcgccg cagcgcgcgc
     1321 gcagaaggcc gaaacgaagc tgatcgttat tcacgacaag gtcgcgcagg cggcgaagga
     1381 ggcgcacgat gggattgccg gagcggctgc gcaggcgcgg ccgggttgtg atgagtctgg
     1441 tgttgtgtct gcctggcgcg acgggcttga gcgcgtgcgt caggccgccg gcgccgctgc
     1501 tgattccgcc ggcggcgcgt cagccgtgcc gctcgccgga cccgcgcaac gtcaggacga
     1561 cgcaggatct ggccgacttc tcgatcgagc aggaacgcgc gctgacgacc tgcgaagccg
     1621 aacgcaaggc gctgatcaag gcgattgacg ggaaatgaag ggcggtaatt ttaagtgccg
     1681 ccgcttcatc agcatcgcat tcatcatttc attgcttaat cgtaaataaa ccccgctggc
     1741 tcgcacctgc ggggttttta ttcatcattg cttttcgggt tgcgcagtct tgtccacttc
     1801 ctgtttccgt ccct
//
LOCUS       DASVLL010000222         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_497593,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000222 DASVLL010000000
VERSION     DASVLL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497593"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..898
                     /locus_tag="VF402_03625"
     CDS             77..898
                     /locus_tag="VF402_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX7799412.1"
                     /translation="MLDADPSIALIRATVQIDQPINADRRMVGTGFLISAPKPGGGVE
                     VVLVTAAHVFEKMPAPQVRLGWRIQNAKGEWVYTPESLTIRAADGPLWYQHPDQDIAV
                     IPVELPDGFREAAAPESWLADDRSFAAQPIEPGAEMMTLGYPHGLSANTAGFPILRAG
                     RVASYPLAPSGRYPTFLIDLTAAPGNSGGPVFMSRAGAEPLVTGVLIKEVEDDGQRLE
                     LGVVADAVFVRQTIDLMLKAQAEGHGPFVRPTAPAAPAPMRTPGTGGDMTGSNPK"
     gene            complement(885..>1636)
                     /locus_tag="VF402_03630"
     CDS             complement(885..>1636)
                     /locus_tag="VF402_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7799413.1"
                     /translation="LMLGLMKAKGEAARAVCMATAAAVDAARMAGDEASRQRHRRRED
                     VLVPIAKAWSTDRAVEAASLGVQVHGGMGFIEETGAAQYYRDARIAPIYEGTNGIQAG
                     DLVGRKLGGDGQAVKELEADIADFLQNKDLAADFNSEVTQLRAALAAFSDATDHLLRC
                     KADAPLDVAAAATTYLTLGGDVAGGWLLLKGAARAKALIDAGDPDAAWLGDRIRIMRL
                     YFAHVLSHAGAHLAAIRSGFGALDGLELTLD"
BASE COUNT          240 a          558 c          583 g          255 t
ORIGIN      
        1 agggcgtatt gacccttcgc ggaaatgccg tcaaatgtcg agagggcttt tgatggccgg
       61 aaggggaggg ggcggcgtgc tggatgcgga cccgagcata gcgctgatca gggcgacggt
      121 gcagatcgat cagccgatca acgcggaccg gcgcatggtc ggcacgggat tcctgatctc
      181 cgcgccgaag cccggcggcg gcgtcgaggt ggtgctggtc accgccgcgc acgtgttcga
      241 aaagatgccc gcgccgcagg tgcgtctggg ctggcgcatc cagaatgcga agggcgagtg
      301 ggtctatacg cccgaatcgc tgaccatacg cgcggcggac gggccgttgt ggtatcagca
      361 tcccgatcag gacatcgccg tcatcccggt ggagttgccc gacggtttcc gcgaggcggc
      421 ggcgccggaa agctggctgg ccgacgaccg cagcttcgcc gcgcagccca tcgaaccggg
      481 cgcggagatg atgacgctgg gctatccgca cggcctgtcg gccaataccg cgggctttcc
      541 cattctgcgc gccgggcggg tcgcctccta tccgctggcg ccgtccggcc gctatccgac
      601 cttcctgatc gacctgacgg cggcgccggg caattccggc gggccggtct tcatgagccg
      661 cgcgggcgcc gaacctctgg tgacgggcgt gctgatcaag gaggtcgagg acgatggcca
      721 gcggctggag ctgggcgtcg tggccgacgc ggtgttcgtg cgccagacga tcgacctgat
      781 gctgaaggcg caggccgaag ggcatggccc cttcgtgcgt ccgaccgcgc ccgccgcgcc
      841 cgcgccgatg cggacgccgg ggaccggcgg cgacatgacc gggtctaatc caaagtaagc
      901 tccagcccgt ccagcgcgcc gaagccgctg cggatcgcgg ccagatgcgc gccggcatgg
      961 ctgaggacat gcgcgaagta caggcgcatg atacggatgc ggtcgcccag ccacgccgcg
     1021 tcgggatcgc ccgcgtcgat cagcgccttc gccctggccg cgcccttgag cagcagccag
     1081 ccgccggcca cgtcgccgcc cagcgtcaga taggtcgtgg ccgccgcggc gacgtcgagc
     1141 ggcgcgtcgg ccttgcatcg cagcaggtgg tccgtggcgt cggaaaaggc ggcgagggcg
     1201 gcgcgaagct gcgtaacctc gctattaaag tctgcggcca agtccttgtt ttgcaaaaaa
     1261 tccgcgatgt cggcttcaag ctctttcacc gcctgtccgt cgccgcccag cttgcgcccg
     1321 accagatcgc ccgcctgaat gccgttcgtg ccttcataga tcggggctat gcgcgcgtcg
     1381 cggtaatatt gcgccgcgcc ggtctcctcg atgaagccca tgccgccgtg gacctgcacg
     1441 cccagcgagg cggcctcgac ggcgcggtcc gtgctccacg ccttggcgat ggggacgagc
     1501 acgtcctcgc gccgcctgtg gcgctggcgg ctggcttcgt cgccggccat gcgcgcggcg
     1561 tcgacggcgg cggcggtcgc catgcagacg gcgcgcgccg cttcgccctt cgccttcatc
     1621 agccccagca tcaggc
//
LOCUS       DASVLL010000223         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_497637,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000223 DASVLL010000000
VERSION     DASVLL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497637"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VF402_03635"
     CDS             <1..966
                     /locus_tag="VF402_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7799414.1"
                     /translation="QDRALAIEAAGLALSPRLQSDAANAGYTQNRWLMLTSDGFEGAH
                     QSTLFFQSGRFIASGDDGQMERDGEGRSTRFLADLPDVADTGRTGAERALAALGARKL
                     DTQKTAVIFDQRVARSLIGPLLGAISGAMIARGSSFLKDRLGRKIFGDAIRIVDDPLR
                     PRGLGSCLFDDEGVTAERRALIEDGVLTTWLLNTSAARQLGLASTGHASRGLAGAPGV
                     SAHNVVLAAGTRAPRDLMRDAGKGVLITSMFGPSLNSDTGDWSAGASGFAFENGEIAY
                     PVNEITVAGNLIDIYARLIPASDLDIRGALDAPSLLVDALSIGGK"
     gene            966..1766
                     /locus_tag="VF402_03640"
     CDS             966..1766
                     /locus_tag="VF402_03640"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX7799415.1"
                     /translation="MSAPVRASDLDLIVDKVRRAGALAQHLKSHGLITKYKADGSIVT
                     NADTEVNLWLKEALLDARPGYGWQSEEEADNAHRLDAARTFVLDPIDGTMGLSRGSPY
                     WTIALAVIENNQPVAAVVYAPDAAELYSAAIGEGATLNGDAIRASRRAELEGAEALGD
                     SRLFGKDIWRLPWPRLSVHSRPSVAYRMACVAAGKADFTLALTPKRDWDVAAATLIAR
                     EAGAVVSDHLGHPYGFNAVDSLKPSLICSGPALFDDIIRRCAHLDTLN"
     gene            1782..2012
                     /locus_tag="VF402_03645"
     CDS             1782..2012
                     /locus_tag="VF402_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX7799416.1"
                     /translation="MSDKQLLHIVIGGELTDLNSTNFKDLSKIEFVGAYPNNAEAVKA
                     WRAKAQSTVDNALMRYFVIHAHKLIDPEAEGH"
BASE COUNT          381 a          793 c          798 g          356 t
ORIGIN      
        1 caggaccgcg cgctggcgat cgaggcggcg ggcctggccc tgagcccccg cctgcaaagc
       61 gacgcggcca acgccggtta cacgcagaac cgctggctga tgctgacctc ggacgggttc
      121 gagggcgcgc atcaaagcac cctgttcttc cagtcgggcc gcttcatcgc cagcggcgac
      181 gacggccaga tggagcgcga cggcgaaggc cgcagcacgc gcttcctcgc cgatctgccc
      241 gacgtcgccg acaccgggcg gaccggggcc gaacgcgcgc tggccgcgct gggcgcgcgc
      301 aagctcgata cgcagaagac ggcggtgatc ttcgatcagc gcgtggcgcg ttcgctgatc
      361 gggccgctgc tcggcgcgat ttcgggcgcg atgatcgcgc gcggctccag cttcctgaaa
      421 gacaggctgg ggcgaaagat attcggcgac gccatacgga tcgtcgacga tccgttgcgt
      481 ccgcgcggcc tcggctcgtg cctgttcgac gacgagggcg tgacggcgga acgccgcgcc
      541 ctgatcgagg acggcgtgct gacgacgtgg cttctgaaca cctccgccgc gcggcaactg
      601 ggactggcct cgaccggcca cgcctcgcgc gggctggccg gggcgccggg cgtgtcggcg
      661 cacaatgtcg tcctcgccgc cgggacgcgc gcgccgcggg atctgatgcg cgacgcgggc
      721 aagggcgtgc tgatcacctc gatgttcggc cccagcctga acagcgacac cggcgactgg
      781 tccgcgggcg cttcgggatt cgcgttcgag aacggcgaga tcgcctatcc ggtcaatgag
      841 atcaccgtgg cgggcaatct gatcgacatc tatgcgcggc tgatcccggc cagcgatctc
      901 gacattcgcg gcgcgctgga cgcgccgtcc ctgctggtcg acgcgctcag catcgggggg
      961 aaatgatgtc cgcccccgtc cgcgcctccg acctcgacct gatcgtcgac aaggtccgcc
     1021 gggcgggcgc gctggcgcag catctgaaaa gccacggcct gatcaccaag tacaaggccg
     1081 acggctcgat cgtcaccaat gccgacaccg aggtcaatct gtggctgaag gaggccctgc
     1141 tggacgcgcg gcccggttac ggctggcaga gcgaggaaga ggccgacaac gcccaccggc
     1201 tcgatgcggc gcgcaccttc gtgctggacc cgatcgacgg cacgatgggc ctgtcgcgcg
     1261 gcagcccgta ctggaccatc gcgctggccg tgatcgagaa caaccagccg gtcgccgccg
     1321 tggtctatgc gcccgacgcc gccgagcttt acagcgccgc catcggcgaa ggcgcgaccc
     1381 tgaacggcga cgcgatccgc gccagccgcc gcgccgaact ggaaggcgcc gaggcgctgg
     1441 gcgattcgcg cctgttcggc aaggatatct ggcgcctgcc gtggccgcgc ctgagcgtgc
     1501 attcgcgccc gtcggtcgcc tatcgcatgg cgtgcgtggc ggcgggcaag gccgacttca
     1561 ccctggccct gacgcccaag cgcgactggg acgtggccgc cgccaccctg atcgcgcgcg
     1621 aggccggggc cgtggtcagc gaccatctgg gccatcctta cggattcaac gccgtcgaca
     1681 gcctcaagcc cagcctgatc tgctcaggtc cggccctgtt cgacgacatc atccggcgct
     1741 gcgcccatct ggacaccctc aactaaaaaa gagccaagct tatgagtgac aaacagcttc
     1801 ttcatatcgt gatcggcggg gaattgaccg atctcaacag cacgaatttc aaggatctga
     1861 gcaagatcga gttcgtcggg gcctatccca acaacgccga agccgtgaag gcgtggcgcg
     1921 ccaaggcgca atcgacggtg gacaacgcgc tgatgcggta tttcgtgatc catgcgcata
     1981 agctgatcga ccctgaagcg gaagggcact gaggggggaa gcaaaggccc cctcccaccg
     2041 cttcgcggtc ccccctcccc gtaaacgggg agggcgaatg tgcctctcct ccaccgttta
     2101 cgggggaggc ggcgtttgcg gagcaaacga cggagggggg aagcggcgcg cgccccccgt
     2161 cttacttatg attggtcaca tcgtccgccg cattatccag attgcgcttg acgttctggg
     2221 cgggcggctg gtcgtcgagc ttgctcgcgg ccttgttcag cccgttcggc agggcggcga
     2281 cggcgtcgcc cgcgcgttcg gcggggccgc ggtggtcctg catcaggt
//
LOCUS       DASVLL010000224         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_227282,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000224 DASVLL010000000
VERSION     DASVLL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227282"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..930
                     /gene="moeB"
                     /locus_tag="VF402_03650"
     CDS             193..930
                     /gene="moeB"
                     /locus_tag="VF402_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX7799417.1"
                     /translation="MTDLTDDELDRYARHIVLREIGGQGQMKLKAARVALVGMGGIGA
                     PAALYLAAAGVGTLGLIDDDHVSLSNLQRQVLYATRDAGASKAESAATHLNDLNPYCE
                     CRVHRLRLDEDNAAQVLGDYDLVIDGCDNFPTRLLVNRVCHALQKPLVSAAIGRFSGQ
                     LATYQGSPCYQCLVPEIPPDAETCERVGVVGALAGVMGTLAALEAVKRITGAGETLQG
                     RLLIFDGLSFESRIVKLAADPACPVCG"
     gene            complement(927..1202)
                     /locus_tag="VF402_03655"
     CDS             complement(927..1202)
                     /locus_tag="VF402_03655"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEX7799418.1"
                     /translation="MTQKKWSHEVTEHSNAMDTRPDIFTSHDPHKIALSLKQSAEASH
                     RRKSSPYRSAMSMLTFYINRAGDNLSDSQRRVLEKAKDELREAFGKA"
     gene            1354..2604
                     /gene="rlmN"
                     /locus_tag="VF402_03660"
     CDS             1354..2604
                     /gene="rlmN"
                     /locus_tag="VF402_03660"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7799419.1"
                     /translation="MAPVLDLSVKPAALPVNITGLTREGLVAALIASETVEPRKAKMR
                     ATQIWRWIHHYGVTDFDLMTDIAKDQRALFKDRFTLARPEIVERQISRDGTRKYLIRM
                     APGIEVESVYIPDVGRAGALCVSSQVGCTLNCSFCHTGTQKLVRNLSAAEIVAQVQVA
                     RDDLGEWPSPKEDRRLSNIVFMGMGEPLYNLDNVADAIDIISDHEGIALSRRRITVST
                     SGVVPELPMLGQRTAAMPAISLHATNDALRDVLVPLNKKYPIAQLMAGIRAYPGLSNA
                     RRVTFEYVMLKGVNDSPAEARELIRLLKGIPAKINLIPFNPWPGTDYQCSDWKTIEAF
                     AAILNKAGYASPIRTPRGRDILAACGQLKSESEKKRASVVRREAQEAEVKAAEVAAET
                     ASEQDSAEGEDHLPYAHLAAGMIG"
     gene            2750..3205
                     /locus_tag="VF402_03665"
     CDS             2750..3205
                     /locus_tag="VF402_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX7799420.1"
                     /translation="MTSDFNFNFLSPEVLRPEMQAFASGFPVTLLHAGVTLLLLLVGA
                     TVYSILTPYKEIQQIREGNSAAAVAYGGVIIGLAIPLAASMAASTSLREILLWGGATI
                     ILQLFVFRLVDFLLTGLPARINEGEVSAAVLLVAAKLAAALVLAAAVAG"
     gene            3269..4027
                     /locus_tag="VF402_03670"
     CDS             3269..4027
                     /locus_tag="VF402_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX7799421.1"
                     /translation="MARALHAPPAPPPPGVGEMSLFATYTPFGAMSIINLPLDNTKVM
                     TGTAFSVARTGEWIVASASVRACPHPFINIGGNLAAPMTLRPIRGVDNYVMGVTDGAG
                     RPLPLADPKEVKPGMRAFMPGFPGGAVGEATARLIGQTLIERSKRGGRNEVALAWAKA
                     GQTLGLKGDLNQLLGGPAIDDASRVVGVTLKESPRRGRIYTSTPQTVLAIANPPERTP
                     DYDSEATVTKRNYGIVADTLRREYRVAQVGCIKS"
BASE COUNT          720 a         1334 c         1397 g          762 t
ORIGIN      
        1 ggtcgagggc gcgaaggggc tggggtagtt agagaaagga tttggccacg aaaggacacg
       61 aaagcaaaag cttcatcggg acggctgaac atcgaaagcc ttggctttga tttcgtgtcc
      121 tttcgtgttt ttcgtggcga aatcttatgc agcctctccc gttgaaggcg gagagggatt
      181 aagtaagcac gcatgaccga cctcaccgac gacgaactgg accgttatgc gcggcatatc
      241 gtgctgcgcg agattggcgg gcaggggcag atgaagctca aggccgccag ggtcgcgctg
      301 gtcggcatgg gcgggatcgg ggcgccggcg gcgctgtatc tggcggcggc gggggtcggg
      361 acgctggggc tgatcgacga cgatcatgtc tcgctgagca acctgcaacg gcaggtgctg
      421 tacgcgacgc gcgacgcggg cgcatcgaag gcggaaagcg cggcgacgca tctgaacgat
      481 ctcaatccgt actgcgaatg ccgcgttcac cggctgcggc tggatgagga caatgcggcg
      541 caggtgctgg gcgattacga tctggtcatc gacggttgcg acaacttccc gacgcggctg
      601 ctggtcaacc gggtctgcca tgcgctgcaa aagccgctgg tgtcggcggc gatcgggcgg
      661 ttttccgggc aactggccac ctatcagggt tcgccctgct atcaatgcct ggtgccggag
      721 attccgcccg acgccgagac gtgcgaacgc gtcggcgtgg tcggggcgct ggcgggcgtg
      781 atggggacgc tggcggcgct ggaggcggtc aagcggatca ccggcgcggg cgagacgctg
      841 caaggacgcc tgctgatctt cgacgggctg agcttcgaga gccgcatcgt caagctggcc
      901 gccgatccgg cgtgtccggt gtgcggttaa gccttgccga acgcctcgcg caattcgtcc
      961 ttggcctttt ccagtacgcg gcgctggctg tcgctgaggt tgtcgcccgc gcggttgatg
     1021 tagaaggtca gcatcgacat ggccgagcgg taggggctgg acttgcgccg gtggctggcc
     1081 tcggcggact gtttcagcga cagcgcgatc ttgtgcggat cgtgcgaggt gaagatatcc
     1141 ggcctggtgt ccatcgcatt ggaatgttcg gtgacctcat gcgaccattt tttctgcgtc
     1201 atggcggctc tccttttgcg gcgaaagcat aggccgaccg tcatgagaaa acgatgcgca
     1261 tcgcacggcg cgccgtcagg aagccgcaag attagacttt tgccgccgct tcctgtatag
     1321 gacgcgcctt cccgaaatat cgagtatccc gccgtggccc ctgtgcttga cctgtccgtg
     1381 aaacccgccg ccttgcccgt gaacattacg ggtctgacgc gcgaggggct ggtggcggcg
     1441 ctgatcgcgt cggagacggt cgagccgcgc aaggcgaaga tgcgcgcgac gcagatatgg
     1501 cgctggatcc accattacgg cgtcaccgat ttcgacctga tgaccgacat cgccaaggat
     1561 cagcgcgcgc tgttcaagga ccgtttcacc ctggcgcgcc cggagatcgt cgagcgccag
     1621 atttcgcgcg acggcacgcg caaatacctg atccgcatgg cgccgggcat cgaggtcgag
     1681 agcgtctata ttcctgacgt gggccgcgcg ggcgccttgt gcgtgtcgtc gcaggtcggc
     1741 tgcacgctca actgctcgtt ctgccatacc ggcacgcaga agctggtgcg caacctgagc
     1801 gcggccgaaa tcgtggcgca ggtgcaggtc gcgcgcgacg acctcggcga atggccgtcg
     1861 cccaaggaag accgccgcct cagcaacatc gtcttcatgg gcatgggcga gccgctctac
     1921 aatctcgaca acgtcgccga cgccatcgac atcatctccg accacgaagg catcgcgctg
     1981 tcgcgccgcc gcatcacggt gtcgacctcc ggcgtcgtgc ccgaactgcc catgttgggc
     2041 cagcgcacgg cggctatgcc ggcgatttcg ctgcacgcca ccaacgacgc cttgcgcgac
     2101 gtgctggtgc cgctgaacaa gaagtatccg atcgcgcagt tgatggcggg catccgcgcc
     2161 tatccgggcc tgtcgaacgc gcgccgcgtg accttcgaat atgtgatgct caagggcgtc
     2221 aacgattccc ccgccgaggc gcgcgagctg atccggctgt tgaaaggcat accggccaag
     2281 atcaacctga tcccgttcaa tccgtggccg ggaaccgatt atcagtgttc ggactggaag
     2341 accatcgagg ccttcgccgc catcctcaac aaggccggtt acgcctcgcc gatccgcacg
     2401 ccacgcggcc gcgacatcct ggccgcctgc ggccagctca agtccgagag cgaaaagaag
     2461 cgcgccagcg tcgtacgccg cgaagcccag gaggcggagg ttaaggcggc ggaagttgcg
     2521 gctgagactg cttcagaaca ggacagcgcg gagggcgaag accacctgcc ctacgcgcac
     2581 ctcgcggcgg gcatgatcgg ctaagccctt accggacaag gcttctccgc tgtcatcata
     2641 attttatcca caggcggtcc cggctggact tgatcttgca cggcgtctgt tccaagactg
     2701 ttcgccgtcc cgcaaacggc gtccggtcgg cgtgacccag gggtaagcga tgacgtccga
     2761 tttcaacttc aacttcctgt cgccggaagt gctgcgcccg gaaatgcagg ctttcgcttc
     2821 cggtttcccg gtgaccctgc tgcacgcggg cgtgacgctg ctgctgctgc tggtcggcgc
     2881 gaccgtctat tccatcctga cgccctacaa ggaaatccag cagatccgcg aaggcaattc
     2941 cgccgccgcc gtcgcctatg gcggggtgat catcggtctg gccataccgc tggccgcgtc
     3001 gatggcggcc tcgacgtcct tgcgcgaaat cctgttgtgg ggcggcgcga ccatcatcct
     3061 gcaactgttc gtctttcgtc tggtcgattt cctgctgacc ggcctgcccg cgcgcatcaa
     3121 cgagggcgag gtgtcggcgg cggtgctgct ggtcgcggcc aagctggcgg cggcgctggt
     3181 gctggcggcg gcggtggcgg gctgaaatga aggccgactg gctcctttac ggcgcggcgc
     3241 tggccgtgct ggtggcggtt ccgggcaagt ggcacgagcg ctccacgcgc cgcccgcgcc
     3301 gccgccgccg ggcgtcggcg agatgtcgct gttcgcgacc tataccccgt tcggcgccat
     3361 gtcgatcatc aacctgccgc tggacaacac caaggtgatg accggcacgg ccttctccgt
     3421 cgccaggacc ggcgaatgga tcgtggccag cgcctcggtg cgcgcatgtc cgcatccctt
     3481 catcaatatc ggcggcaatc tggccgcgcc gatgacgctt aggccgatcc ggggcgtcga
     3541 taactatgtg atgggcgtca ccgacggcgc cgggcggccg ctgccgctgg ccgatccgaa
     3601 agaggtcaag ccgggcatgc gcgctttcat gccgggcttt cccggcggcg cggtcggcga
     3661 ggcgacggcg cggctgatcg gccagaccct gatcgagcgc tccaagcgcg gcgggcgcaa
     3721 cgaggtggcg ctggcctggg ccaaggcggg ccagacgctc ggcctcaagg gcgacctgaa
     3781 ccaactgctc ggcggccccg ccatcgacga tgcgtcgcgc gtcgtcggcg tgaccctgaa
     3841 ggaatccccg cgccggggcc gcatctacac ctcgacgccg caaaccgtgc tggccattgc
     3901 caacccgccg gagcgcacgc ccgactacga ctccgaagcc accgtcacca aacgcaatta
     3961 cggcatcgtg gcggacactt tacgccgcga ataccgcgtg gcgcaggtgg gctgcatcaa
     4021 gtcgtagttt tttcagccac gaaacgaagt tcggcgaagc caaaggcgca aaggaagcga
     4081 agcttcattc ggatcgctat tcgcatagcc atgcgtgatt gggcttcgcc aaaccttttt
     4141 cgtgtctttt cgcgcctttg gcttcaccga acttcgtttc gtggctaacg tctttcaatg
     4201 tccacaaacg ccc
//
LOCUS       DASVLL010000225         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_335471,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000225 DASVLL010000000
VERSION     DASVLL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335471"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>2477
                     /locus_tag="VF402_03675"
     CDS             102..>2477
                     /locus_tag="VF402_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7799422.1"
                     /translation="MTAFPDLIRQDGALTLSGCPEGYDSLIMADIARGAGLGLFVARD
                     FSRMNAVLEALRFFAPDLETLTFPAWDCLPYDRMSPTPGVVAQRMFALSRLAERKDGA
                     APLLVVATAAAAMQRVPPRAVIDEGRLSLRPGQTLDPANLETYFLRHGYTRVSTVSER
                     GEFAVRGGLLDVFSPTHDEPMRLDFFGDSLESIRSFDAETQRSLSQIKRLDFTAVSEI
                     RLDEAGVSRFRQGYLENFGASADDPMYAALSSGIRRQGVEHLLPLFYDRLDTLFDYLT
                     PDAVVMLDALAENAVAERLASAQDAFSLRRDAAEGRYRALPPERLYLSEADWRRTLGD
                     FRLRRFEPFAHEGGVDMGGRAGRDFAEARRLDATNLFTTVTDHARALAKQGKRVLFAS
                     WTDGSSDRLGQMLADHGLEGMRLAKDWPDAQSFGVAGTKVKPIQRAVIPLDHGFETES
                     LAVISETDILGDRLARPRKRRRAQNFLAEASSLSPGDLVVHIEHGIGRYEGLKTLDVA
                     GAPHDCLDLQYANDAKLYLPVENIDLLTRYGADSETAQLDRLGSASWQARKAKAKERL
                     REMADGLIQLAAARATKEGPQIDPPSGLYDEFCAQFPYEETDDQLSAIADVLEDLSRG
                     HPMDRLICGDVGFGKTEVALRAAFVVAMSGQQVAVVCPTTLLARQHFKTFSQRFQGWP
                     IRVRQLSRLVGRKEADETRAGLKAGEIEVVIGTHALLSEQVAFRDLGLVIVDEEQHFG
                     VKHKEKLKTFRADVHMLALSATPIPRTLQMALSGIRDMSIIATPPIDRIAVR"
BASE COUNT          413 a          807 c          842 g          415 t
ORIGIN      
        1 ggatcatcga acgcgatccg accccggttg agcatcaatc ggaaatcatg acggaactca
       61 acagctatta caaaacagct tacacaaaac tttagtctgg tatgaccgct tttcccgatc
      121 tgatccgcca ggacggcgcg ctgaccctca gcgggtgccc cgaaggctat gacagcctga
      181 tcatggccga catcgcgcgc ggcgcgggcc tcggcctgtt cgtggcgcgc gatttcagcc
      241 gcatgaacgc cgtgctggag gcgctgagat tcttcgcgcc cgatctggag acgctgacct
      301 ttccggcgtg ggactgcctg ccttacgacc ggatgagtcc gacgccgggc gtggtcgcgc
      361 agcgcatgtt cgccttgtcg cgattggcgg aacgcaagga cggcgccgcg ccgctgctgg
      421 tggtcgccac ggcggcggcg gcgatgcaga gggttccgcc gcgcgcggtg atcgacgagg
      481 ggcgtctgag cctgcggccc ggccagacgc tggacccggc gaatctcgaa acctatttcc
      541 tgcgccacgg ctatacgcgc gtctcgaccg tgtcggagcg cggcgaattc gcagtgcgcg
      601 gcggtctgct cgacgtcttt tcgccgacgc atgacgagcc gatgcggctc gacttcttcg
      661 gcgacagcct ggaatcgatc cgcagcttcg atgcggaaac ccagcgctcg ctgagccaga
      721 tcaagcgcct cgacttcacc gccgtgtcgg aaatccggct cgacgaggcg ggcgtcagcc
      781 gcttccggca gggctatctg gaaaatttcg gcgcatccgc cgacgatccg atgtacgcgg
      841 cgctgtcgtc gggcatccgg cgtcagggcg tcgagcatct gctgccgctg ttttacgacc
      901 gtctcgacac gctgttcgat tatctgacgc cggatgcggt ggtcatgctc gacgcgctgg
      961 ccgaaaacgc ggtcgcggaa cggttggcct cggcgcagga cgcgttcagc ctgcgccgcg
     1021 acgccgccga gggccgctat cgcgccctgc cgccggagcg gctctatctc agcgaggccg
     1081 attggcgccg gacgctgggc gatttccgcc tgcgccgttt cgagccgttc gcgcacgaag
     1141 gcggcgtcga tatgggcggg cgcgcgggcc gcgatttcgc cgaggcgcga aggctggacg
     1201 ccaccaacct gttcaccacg gtgaccgatc atgcgcgcgc tctggccaaa cagggcaagc
     1261 gcgtcctgtt cgcgtcgtgg acggacggct cgtccgaccg gctggggcag atgctggccg
     1321 atcacgggct ggaggggatg cggctggcga aggactggcc ggacgcgcaa agcttcggcg
     1381 tggccggaac gaaggtcaag ccgatccagc gcgccgtcat cccgctcgac cacggcttcg
     1441 agacggaaag cctggccgtc atttccgaaa ccgatatctt aggcgacagg ttagcccgtc
     1501 cgcgtaaacg tcggcgcgcg cagaattttc tggcagaagc gtcatcgttg tcgcccggcg
     1561 atctggtcgt gcatatcgag catggcatcg gccgttacga ggggctgaaa acgctcgacg
     1621 tcgccggggc gccgcacgat tgcctcgacc tgcaatacgc caacgacgcc aagctgtacc
     1681 tgccggtgga gaatatcgac ctgctgacgc gctacggcgc cgacagcgag acggcgcagc
     1741 tcgaccggct cggctcggca agctggcagg cgcgcaaggc caaggcgaaa gagcgcctgc
     1801 gcgagatggc cgacggcctg atccagctgg cggcggcgcg cgccaccaag gaagggccgc
     1861 agatcgatcc gccttcgggc ctctatgacg aattctgcgc ccagttcccg tatgaggaga
     1921 ccgacgacca actcagcgcc atcgcggacg tgctggagga tctgtcgcgc gggcatccga
     1981 tggaccggct gatctgcggc gacgtcggct tcggcaagac cgaggtggcg ctgcgggcgg
     2041 ccttcgtggt ggccatgtcg ggccagcagg tcgccgtggt ctgcccgacc accctgctgg
     2101 cgcggcagca tttcaagacc ttcagccagc gctttcaggg ctggccgatc cgggtgcggc
     2161 aattgtcgcg gctggtcgga cgcaaggagg ccgacgagac gcgcgccggt ctgaaggcgg
     2221 gcgagatcga ggtggtgatc ggcacgcacg ccctcttgtc cgagcaggtg gcgttccgcg
     2281 atctcggcct cgtcatcgtc gacgaggagc agcatttcgg cgtcaagcac aaggaaaagc
     2341 tcaagacctt ccgcgccgat gtgcatatgc tggccttgtc cgcgacgccg ataccgcgca
     2401 ccttgcagat ggccttgtcg ggcattcgcg acatgtcgat catcgccacg ccgccgatcg
     2461 accgcatcgc cgtgcgc
//
LOCUS       DASVLL010000226         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_13462, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000226 DASVLL010000000
VERSION     DASVLL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13462"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VF402_03680"
     CDS             complement(<1..1472)
                     /locus_tag="VF402_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7799423.1"
                     /translation="MPAVMSAPMPGVAHAEKLTSLATEAIRSASQDYYVFIIVFLLAS
                     AIIVPLAQRFKVSSVLGFLLVGVLMGPDALGRLSHHLPGLDAFSMIRTHAVHRLAELG
                     VVFLLFSIGLELTFERLKAMRRMVLGLGASQIVLSSIALAVVFFVMGRSVVGSTLLGM
                     ALALSSTAVIIPVLADRKALNSASGRSVFAVLLAQDVAVAPIMVTAMILTGSPESPGG
                     HGVGAQGLLALIPALIGMGVLILGGRWLLRPLFNTVALSRSRELFMAACLLVILLAGQ
                     VATMTGMSMALGAFVAGVLLAETEYRREIEVMIEPFKGLLLGLFFVTVGARLDFGTVM
                     AQPVLVLGLAAGLIALKFAVIFPLARAFGLSTPRAIETAAVLGPAGEFAYVIIDEAIN
                     RHVVPEAFGHAIILSATLSLFSVPLLAAVAGRVAARFDRQRNAPPPEAPDVVSDADPV
                     IIVGFGRVGELVADMLAAHDIPFTVVDFNPQVTKRARARGL"
     gene            1696..>3791
                     /locus_tag="VF402_03685"
     CDS             1696..>3791
                     /locus_tag="VF402_03685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX7799424.1"
                     /translation="MMTPSRLLAATALAGTLALLSVAPAATAGCIDAHCHLLSLESFD
                     GVIPANAADMQGGADGASTEAPRYGSWGIDLGGMDPSVKPGDNFFDYVNGKAVAKMVI
                     PADRSSYGSFVQLIDLSEARSRAIVTGLAARGDLSGDDAKIAALYKAMMDDKTVEALD
                     VAPVKPLLADIAAISDKAGMAAWMGKTSGTLGSSFFGLYVDADADNPKINVLYAGQSG
                     LGLPDRDYYLKDAFADKKAKYQAYVAQLLGMAGYPNADKAAADIVAMETEIAKVSWSR
                     IEQRDPIKTYNPMTEAEVATMAPGFDWAGFFAGAGVANAAKVVVGEKSAFPAIAKVFD
                     ATPLDTIKAWETFRTLDQAAPYLSERFVTAQWTFRAHELSGAQEQRPRWKRAIGLVER
                     AMGEPLGRDYVAQYFPPESKAKMEQLVADLQTALRARIQNLTWMTPETKAKALEKLSK
                     FGVKIGYPDKWRDYSGLEVKADDLVGDVQRSAAFEWDYNRNKIDKPVDPTEWDMTPQT
                     VNAYYNPTRNEIVFPAAILQPPFFDPQADMAVNYGGIGGVIGHEITHGFDDQGRHYDG
                     DGKLADWWTAADSAKFDAEAKKYGEQYDQFEPLPGVHVQGGLTMGENIADLGGNLLGY
                     DAYHLYLKRQGKTEGPVLNGFSGDQRVFMGFAQVWRSKYRDDAIKQQVTTDPHSPAVF
                     RVIGPVRNIDAWYKAF"
BASE COUNT          716 a         1315 c         1228 g          532 t
ORIGIN      
        1 aggccgcgcg cgcgggcgcg cttggtgacc tgcgggttga aatcgaccac ggtgaacggg
       61 atgtcgtgcg ccgcgagcat atcggcgacc agctcgccga cgcggccgaa gccgacgata
      121 atcaccggat cggcgtcgct caccacgtcg ggcgcctcgg gcggcggcgc gttgcgctgg
      181 cggtcgaagc gcgcggcgac gcggcccgcc acggcggcca gcagcggcac gctgaacagc
      241 gacagggtag ccgacaggat gatggcgtgg ccgaaggctt cgggcacgac gtggcggttg
      301 atggcttcgt cgatgatgac ataggcgaat tcgcccgccg gccccagcac cgccgccgtt
      361 tcgatggcgc gcggcgtcga cagcccgaag gcgcgcgcca gcggaaagat gaccgcgaat
      421 ttcagcgcga tcagccccgc cgcgaggccc agcaccagaa ccggctgcgc catcaccgtg
      481 ccgaaatcga gccttgcccc gaccgtgacg aagaacaggc ccagcagcag ccccttgaac
      541 ggctcgatca tcacctcgat ctcgcgccgg tactcggttt cggccagcag cacgcccgcg
      601 acgaacgcgc ccagcgccat cgacatgccg gtcatggtcg ccacctgtcc cgccagcagg
      661 atgaccagca ggcaggccgc catgaacagt tcgcggctgc gcgacagcgc caccgtgttg
      721 aacagcggac gcagcagcca gcgcccgcca aggatcagca cgcccatgcc gatcagcgcc
      781 gggatcagcg ccagcagccc ctgcgcgccg acgccgtgcc cgccggggct ttcgggcgag
      841 ccggtcagga tcatcgccgt caccatgatc ggcgccaccg ccacgtcctg cgccagcagc
      901 acggcgaaca cgctgcggcc cgaggccgag ttcagcgcct tgcggtcggc cagcaccgga
      961 atgatcaccg ccgtcgagga cagggccagc gccatgccca gcagggtcga gccgaccacg
     1021 ctgcggccca tgacgaagaa caccacggcc agcgcgatcg aggacagcac gatctgcgag
     1081 gcgcccagcc ccagcaccat gcgccgcatc gccttaaggc gctcgaaggt cagctccagc
     1141 ccgatggaga acagcaggaa caccacgccc agctccgcca gccggtgcac ggcgtgggtg
     1201 cggatcatcg agaacgcgtc cagccccggc aggtggtggc tgagccgccc cagcgcgtcc
     1261 ggccccatca gcacgccgac cagcaggaag cccagcacgc tcgacacctt gaagcgctgc
     1321 gccagcggca cgatgatcgc cgaggccagc aggaacacga tgatgaagac atagtagtcc
     1381 tgcgacgcgc tgcgtatcgc ctcggtggcc agcgaggtca gtttctcggc gtgcgcgacg
     1441 ccgggcatgg gggcggacat gacggctggc atgggggcgc ggatctcctt ttcatgtctg
     1501 ttcgtgaggc gcttttggaa actatggggt atgcgcgccg caggggaaac ccccgcgagg
     1561 tattttttca cgccaagatg acgaaaaatt aatgtttgca aaaggccgtt tggcgcttag
     1621 acgttacatc gttacaaatt ttatcagttc cggcccgtcc ggcctgagcc tcgttcatct
     1681 cgcaaagaag gacacatgat gacgccctcc cgtctcctcg ccgccaccgc cctcgcaggg
     1741 acgctggccc tcctgagcgt cgcgcccgcc gccaccgccg gctgcatcga cgcccattgc
     1801 catctgctga gcctggaaag cttcgacggc gtgatcccgg ccaacgccgc cgacatgcag
     1861 ggcggcgcgg acggcgcctc gaccgaggcg ccgcgttacg gcagctgggg catcgatctc
     1921 ggcggcatgg acccgtcggt caagccgggc gacaacttct tcgattacgt caacggcaag
     1981 gccgtggcga aaatggtcat ccccgccgac cgcagctcct acggcagctt cgtgcaactg
     2041 atcgacctgt ccgaggcgcg ctccagggcg atcgtcaccg ggctggccgc gcgcggcgac
     2101 ctgagcggcg acgacgccaa gatcgccgcc ctctacaagg cgatgatgga cgacaagacg
     2161 gtcgaggcgc tggacgtcgc gccggtcaag ccgctgctgg ccgacatcgc cgccatctcc
     2221 gacaaggcgg gcatggccgc gtggatgggc aagacgtccg gcacgctggg ctcgtccttc
     2281 ttcggcctct atgtcgacgc cgacgccgac aatccgaaga tcaacgtgct gtacgccggt
     2341 cagtcgggcc tgggcctgcc ggaccgcgac tactatctga aggacgcctt cgccgacaag
     2401 aaggccaagt atcaggccta tgtcgcccag cttctcggca tggcgggcta tccgaacgcg
     2461 gacaaggccg ccgccgacat cgtggcgatg gagacggaga tcgccaaggt cagctggtcg
     2521 cgcatcgaac agcgcgatcc gatcaagacc tataacccga tgaccgaggc cgaggtcgcc
     2581 acgatggcgc cgggcttcga ctgggccggt ttcttcgccg gcgccggcgt cgccaacgcc
     2641 gccaaggtgg tcgtcggcga aaagagcgcc ttcccggcca tcgccaaggt gttcgacgcc
     2701 acgccgctcg acacgatcaa ggcgtgggag acgttccgca cgctggatca ggccgcgccc
     2761 tacctgtccg aacgcttcgt gaccgcgcaa tggaccttcc gcgcgcacga actgtccggc
     2821 gcacaggagc agcgtccgcg ctggaagcgg gccatcggcc tggtcgagcg cgcgatgggc
     2881 gagccgctgg gccgcgacta tgtggcgcag tatttcccgc ccgaatccaa ggccaagatg
     2941 gagcagctcg tcgccgacct gcaaaccgcc ttgcgcgccc gcatccagaa cctgacctgg
     3001 atgacgccgg aaaccaaggc gaaggcgctg gagaagctgt ccaaattcgg cgtcaagatc
     3061 ggctatcccg acaagtggcg cgactatagc gggcttgagg tcaaggccga cgacctcgtg
     3121 ggcgacgtgc agcgttcggc ggcgttcgag tgggactata accgcaacaa gatcgacaag
     3181 ccggtcgatc cgaccgagtg ggacatgacg ccgcagacgg tcaacgccta ctacaatccg
     3241 acgcgcaacg agatcgtctt cccggcggcc atcctgcaac cgcccttctt cgatccgcag
     3301 gccgacatgg cggtcaatta cggcggcatc ggcggcgtga tcggccacga aatcacccac
     3361 ggcttcgacg atcagggccg ccactacgac ggcgacggca agctggccga ctggtggacc
     3421 gccgccgatt cggccaagtt cgacgccgag gccaaaaagt acggcgaaca gtacgaccag
     3481 ttcgagccgc tgcccggcgt gcatgtgcag ggcggcctga ccatgggcga gaacatcgcc
     3541 gatctgggcg gcaacctgct cggctacgac gcctatcacc tatacctgaa gcgtcagggc
     3601 aagacggaag gcccggtgct gaacggcttc agcggcgacc agcgcgtctt catgggcttc
     3661 gcgcaggtct ggcgctccaa gtaccgcgac gacgccatca agcagcaggt caccaccgac
     3721 ccgcactcgc ccgccgtctt ccgcgtcatc ggcccggtgc gcaatatcga cgcctggtac
     3781 aaggcgttca a
//
LOCUS       DASVLL010000227         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_120587,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000227 DASVLL010000000
VERSION     DASVLL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120587"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /gene="infC"
                     /locus_tag="VF402_03690"
     CDS             <1..228
                     /gene="infC"
                     /locus_tag="VF402_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX7799425.1"
                     /translation="IDKHDYEVKAKAMHRFFEEGDKVKVTLRFRGREMAHPELGMKLL
                     NQVKADFEPTTRVEYEPRMEGKQMIMILAPK"
     gene            314..661
                     /locus_tag="VF402_03695"
     CDS             314..661
                     /locus_tag="VF402_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7799426.1"
                     /translation="MNFLKFDRCDLPEPEHGAPAPDKVIDGDPRFTSWTLDSTAPNTF
                     AGYWAATPGAWRVAYDEWEYCTLVEGHAVVVEDGHAPVDLRPGDHFIFRPGYQGQWRV
                     VEPVLKTFVVILP"
     gene            complement(688..1968)
                     /locus_tag="VF402_03700"
     CDS             complement(688..1968)
                     /locus_tag="VF402_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HEX7799427.1"
                     /translation="MSDDVTDGGLNWLYLDMNSYFASCEQQDDARLRGRPVIVVPVMS
                     DFTSAIAASQEAKALGVRTGTRVREAKERIPGLLIREARPDRYVELHDRILAEVDHVL
                     PLDRVCSIDEVACRLTGPQRHEAAARALGHRIQRRILENVGECLTASVGIAPSRLLAK
                     TAADMKKPLGLTVLRLDRLPGDLLGLKLDDFAGIGGSMLKRLNAARIHDVRQLWETPP
                     SRLRRIWGGVTGEQFWYALHGVDPPETETQRSSISHGHVLPAELRPLAQARTVARRLT
                     AKCGSRLRRMGYKCTGIYLSLSGGGRDGEGQRRRAGAEARFELTSDSFVMLRALEAAW
                     TEAVARLRDDRARKVSVTCLGLRATDAPPDLFGWTPEAREDAKHMKLLGALDSLNQKF
                     GKDAITIGPRTKLHDFVGAKIAFNRIPEKPEFWE"
     gene            2082..2648
                     /locus_tag="VF402_03705"
     CDS             2082..2648
                     /locus_tag="VF402_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX7799428.1"
                     /translation="MCSTAPRPSPVLTLSLCALLLLSGCGRQRFVVTTDSATNGRFHQ
                     PQFASTGGCTGANASPHLSWSGAPKAAQSFAVTMFDPDAKTGHGGFWHWAIFNLPATV
                     NDLPDNASGAPARLPPSATQARNDYGDVGYGGPCPPAGEDHRYVITVWALKAPALLVA
                     PTASAQDAAAQIRANAVAHTQITVRAGN"
BASE COUNT          455 a          973 c          868 g          439 t
ORIGIN      
        1 atcgacaagc acgattacga ggtcaaggcc aaggcgatgc accgcttctt cgaggaaggc
       61 gacaaggtca aggtgacctt acgcttccgc ggccgcgaaa tggcgcaccc ggagctgggc
      121 atgaaactgc tcaatcaggt caaggccgat ttcgagccga ccacgcgcgt cgagtacgag
      181 ccgcgcatgg aaggcaagca gatgatcatg atcctggcgc cgaagtaaga gacgcctggc
      241 gcgccgatca cgcatatgcg ctttgcaaaa ccctccgatc cgtcggaggg tttttgcgtg
      301 atgggagcct tgcatgaact ttctgaaatt cgaccggtgc gacctgcccg aaccggaaca
      361 cggcgcgccc gccccggata aggtgatcga cggcgatccg cgctttacgt cgtggacgct
      421 ggattccacc gcgcctaaca ccttcgccgg ttactgggcc gcgacgccgg gggcgtggcg
      481 ggtcgcctac gacgaatggg agtattgcac cctggtcgag ggccacgccg tcgtcgttga
      541 ggacggccac gcgccggtcg atctgcggcc cggcgatcat ttcatcttcc ggcccggcta
      601 tcaggggcaa tggcgggtgg tggagcctgt gctcaagacg tttgtggtga tcctgccgta
      661 ggaagacgcc ccgccaccgc atctcgccta ctcccaaaac tccggcttct ccggtatgcg
      721 gttgaacgcg atcttggcgc cgacgaagtc gtgcaatttg gtgcgcgggc cgatggtgat
      781 ggcgtccttg ccgaacttct ggttcaggct gtcgagcgcg cccagcagct tcatgtgttt
      841 cgcgtcctcc cgcgcctcgg gcgtccagcc gaacaggtcg ggcggcgcgt ccgtcgcgcg
      901 cagccccaga caggtcaccg acaccttgcg cgcgcgatcg tcccggagcc gcgccacggc
      961 ctccgtccac gccgcctcca gcgcgcgcag catgacgaag ctgtccgagg tcagctcgaa
     1021 gcgcgcctcg gccccggccc gccgccgctg gccctcgccg tcgcgccctc cgccgctcag
     1081 cgacagatag atccccgtgc atttatagcc catgcggcgc aggcgcgagc cgcacttggc
     1141 ggtcagccgc cgcgccaccg tccgcgcctg cgccagcggc cgcaactcgg cgggcaggac
     1201 gtggccgtgg ctgatcgacg aacgctgcgt ctccgtctcg ggcggatcga cgccgtgcag
     1261 cgcgtaccag aactgctcgc ccgtcacgcc gccccagatg cggcgcaggc gcgacggcgg
     1321 cgtctcccac aattgccgca cgtcatggat ccgcgccgcg ttcagccgtt tcagcatcga
     1381 gccgccgatc ccggcgaaat cgtccagctt cagccccagc agatcgccgg gcagccggtc
     1441 gagacgcagc acggtcaggc ccagcggctt cttcatatcg gcggcggtct tggccagcag
     1501 ccgcgacggc gcgatgccga ccgaggcggt cagacattcg ccgacgtttt cgagaatccg
     1561 ccgctggatg cggtggccca gcgcgcgcgc cgccgcctca tgccgctgcg gccccgtcag
     1621 gcggcaggcg acttcgtcga tcgagcagac ccggtcaagc ggcagcacat gatcgacctc
     1681 ggccagaatc cggtcgtgca gttcgacata gcggtccggg cgcgcctcgc ggatcagcag
     1741 gcccggaata cgctctttcg cctcgcgcac gcgcgttccg gtcctgacgc ccagcgcctt
     1801 ggcctcctga ctggcggcga tggcgctggt gaagtcgctc atcaccggca cgacgatgac
     1861 cggccgcccg cgcaggcgcg cgtcgtcctg ctgctcgcaa ctggcgaaat agctgttcat
     1921 atcgagatac agccagttca gcccgccatc ggtcacatcg tcgctcatgg cgcgaacata
     1981 aagtgaacat ttgtccacgt caagcgcgcg gcgcttacga aacccccatt gcattttgat
     2041 ccgattaccg gacaatcggg cggatgacag gaggtcgttc catgtgctcc accgctcccc
     2101 gcccgagtcc ggttctgacc ttgagcctgt gcgccctgct gctcctgagc ggctgcggac
     2161 ggcagcgatt cgtcgtcacg accgacagcg cgaccaacgg gcgctttcat caaccgcagt
     2221 tcgccagcac gggcggctgc accggcgcca acgccagccc gcacctgagc tggtccggcg
     2281 cgccgaaggc cgcgcagagc ttcgccgtca ccatgttcga tcccgacgcg aagaccggtc
     2341 acggcggctt ctggcactgg gcgattttca acctgcccgc cacggtgaac gacctgcccg
     2401 acaacgcctc cggcgcgccc gcgcgcctgc cgcccagcgc gactcaggcg cgcaacgatt
     2461 acggcgacgt cggctatggc ggcccctgcc cgcccgccgg ggaggaccac cgctatgtca
     2521 tcaccgtctg ggcgctcaag gccccggccc tgctggtcgc gccgaccgcg tcggctcagg
     2581 acgcggcggc gcagatccgc gccaacgccg tcgcccatac ccagatcacg gtgcgcgcgg
     2641 ggaactgagt cgtgaaccgg aacgggcgaa gataagtttc gcattgcgaa acaaatcggc
     2701 tataaaccgc gcatgtcgaa tttgaacgcc cggtc
//
LOCUS       DASVLL010000228         4683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_174866,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000228 DASVLL010000000
VERSION     DASVLL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174866"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VF402_03710"
     CDS             <1..1126
                     /locus_tag="VF402_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7799429.1"
                     /translation="LAKPYEALDWDEALKIAFPVVQDKAMRYAAGQGMPVEVAALAKG
                     YQTTLGAPTKLRTAPVLKRRIRLAVMGAGGVGAKFLERCLEWSDLIEVDRVLVRDPGK
                     RRDHPLAARFTSDARAFVRDDVDVFVDIGTGVSPSAELLEGFLKAGVSVTSANKQAVA
                     AAGDRLRAAAKASGAMLTYSASVGGGAPIIEALKRAVKDHKIKAFCGVLNGTSNFVID
                     QVEAGKSFDAAMDAARRLGFAEPDSTADLDGTDVGAKLKLLREIAFPGETPAHIEVGA
                     ITAESLVIPQGKGLRYVARCYRTQHGLEASMKLEALDADHYLVGARGEQNRAIIELEN
                     SGEKGGETWCVTGKGAGAWPTTEALLADVLQIAREHPLKA"
     gene            1126..1479
                     /locus_tag="VF402_03715"
     CDS             1126..1479
                     /locus_tag="VF402_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX7799430.1"
                     /translation="MTPDRANAFCAALPASAHVVQWGGADVWKVGGKVYVICREENGH
                     FAASFKVSEMAFEILREHPDCRPAPYLASRGMRWIQYYRGDSLRDGEVLDLIAESHRI
                     VAAGLPKKTRAALGL"
     gene            1476..2312
                     /gene="rlmJ"
                     /locus_tag="VF402_03720"
     CDS             1476..2312
                     /gene="rlmJ"
                     /locus_tag="VF402_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX7799431.1"
                     /translation="MNYRHGYHAGNFADLAKHAAVLALLRAMRDGGPLLVVDTHAGAG
                     AYDLSDAAFARSKEAEAGVQTLLAGDAPDSLGPLADYVRIKNAQAGFRGRIGLYPGSP
                     MLALDHLRAGDAWLGCELREDDFEALRERLGRRGEAVRKDGYKVAVAQAQDARRLLYL
                     IDPPFERPDDYDRITAGVHDILTLRPDAVFLIWLPLKDLETFDSWLRQMEREVLDVSE
                     ASLLVAEVRLRPLTNPMKMNGCALACLNAPEGFAAQLAAIVADVAAVLGEDGALGRTR
                     LL"
     gene            2421..2909
                     /locus_tag="VF402_03725"
     CDS             2421..2909
                     /locus_tag="VF402_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799432.1"
                     /translation="MTSATETPFYLMTPPVMQPEDVRKASERMMNLWLGAMSPLWAPF
                     WAASSFGVGVWAVSHNLKPAEGLLTELPAADGWLSLFRSWGLEAGELHEKAVEAVGNA
                     AEKAESSIEAASVEAAPAEAVAVDPVTEAPVVTPEAAPEPVAPKPIARAVSAARKPRA
                     KT"
     gene            complement(2906..3820)
                     /locus_tag="VF402_03730"
     CDS             complement(2906..3820)
                     /locus_tag="VF402_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7799433.1"
                     /translation="MSRPASRPLHVIGLSLAVPALIVALICLSDPRPTWLYMLDIFTV
                     PLLSACVALTAVLALLRQRLAAVVALAACLVLFAAAAPQILPPRPPAKDGAPALHVVF
                     ANLYFRNPDPGRLAAWAEAQRADIVVTAETTPRLWEMLRARMDAHYPYANQQGEIAIF
                     ARYPFPYQRHPDGHFGVAAMRTPLGPLRLIGAHFDHPDSHREPRRPGFDARLRQRLDA
                     GDPAATLLVGDFNSDMSAYMLKGLARDYRLTALPALNGSWPSPLPGLLRLSIDNALAG
                     ADWRLSGRVVGPDIGSDHRPIAFTLQRN"
     gene            3891..4370
                     /locus_tag="VF402_03735"
     CDS             3891..4370
                     /locus_tag="VF402_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX7799434.1"
                     /translation="MRARTHEDILKIHDSVDTVKRLSDRIIGIGPINLIGLDGILSWV
                     PVPGLDLAYSVLAGGFILVQGARARCDATTLVAAALVLAVDAGLSLGDTVIPVLGAAA
                     DTLFQGQLYAAHMIQRNIDKTHYVEGGEAEARRNGDHARHVADMRAMKGKTRVVYLG"
     gene            4439..>4683
                     /locus_tag="VF402_03740"
     CDS             4439..>4683
                     /locus_tag="VF402_03740"
                     /inference="COORDINATES: protein motif:HMM:NF022436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEX7799435.1"
                     /translation="MSDLTPRLCLPLIGDHAQKQLVMNAALMRLESLVQARVVSRSTA
                     AEPSAPADGDAYILPAGASGATWGGLSAGGVVRAEGGV"
BASE COUNT          743 a         1485 c         1741 g          714 t
ORIGIN      
        1 tctggccaag ccctacgagg cgctggactg ggacgaggcg ttgaagatcg ccttcccggt
       61 ggtgcaggac aaggcgatgc gctacgccgc cgggcagggc atgccggtcg aggtcgcggc
      121 gctggccaaa ggctatcaga ccacgctggg cgcgccgacg aagctgcgca cggcgccggt
      181 cctgaaacgc aggatccgcc tcgccgtcat gggcgcgggc ggggtcgggg cgaaattcct
      241 cgaacgctgc ctggagtgga gcgacctgat cgaggtcgac cgcgtgctgg tgcgcgatcc
      301 gggcaagcgc cgcgatcatc cgctggcggc gcgcttcacc tcggacgcgc gcgccttcgt
      361 gcgcgacgac gtcgatgtgt tcgtcgacat cggcacgggc gtgtcgccgt cggcggagct
      421 gctggaaggc ttcctgaagg cgggcgtcag cgtgacctcg gccaacaagc aggccgtggc
      481 ggcggcgggc gaccgcctgc gcgcggcggc gaaagcctcc ggcgccatgc tgacctattc
      541 ggccagcgtc ggcggcggcg cgccgatcat cgaggcgctc aagcgcgcgg taaaagatca
      601 taagatcaag gccttctgcg gcgttcttaa cggcacgtcg aacttcgtca tcgatcaggt
      661 cgaggcgggc aagagcttcg acgccgcgat ggacgcggcg cgccgtctgg gcttcgccga
      721 gccggattcg accgccgatc tcgacggcac cgatgtcggc gcgaaactga agctgctgcg
      781 tgaaatcgcc ttccccggcg aaacgcccgc gcatatcgag gtcggggcga tcaccgcgga
      841 atcgctggtc atcccgcagg gcaaggggct gcgctacgtc gcgcgctgct accgcacgca
      901 acacgggctg gaggcgtcga tgaagctgga ggcgctggac gccgatcact atctggtcgg
      961 ggcgcgcggc gagcagaacc gcgcgatcat cgaactggaa aattctggtg agaagggggg
     1021 cgagacctgg tgcgtgaccg gcaagggcgc gggcgcgtgg ccgaccaccg aggcgcttct
     1081 ggccgacgtg ttgcagatcg cgcgcgagca tccgcttaaa gcctgatgac gcccgaccgc
     1141 gccaacgcct tctgcgccgc cttgcccgcc agcgcgcacg tcgtgcagtg gggcggggcg
     1201 gacgtatgga aggtcggcgg caaggtctat gtcatctgcc gcgaggaaaa cggccatttc
     1261 gccgccagtt tcaaggtctc ggagatggct tttgagattt tacgcgaaca tcccgactgc
     1321 cgccccgcgc cctatctggc gtcgcgcggc atgaggtgga ttcagtatta tcgcggcgac
     1381 tccttacgcg acggcgaggt gctggacctg atcgcggagt cgcaccggat cgtggcggcg
     1441 gggctgccga agaagacgcg ggcggcgttg ggactatgaa ttatcgtcac ggctatcatg
     1501 cgggcaattt cgccgatctg gccaagcacg ccgccgtgct ggccctgctg cgggcgatgc
     1561 gcgacggcgg gccgctgctg gtggtggaca cccatgcggg ggcgggcgcc tacgatctga
     1621 gcgacgcggc gttcgcgcgt tcgaaggagg ccgaggcggg cgtgcagacc ctgctggcgg
     1681 gcgacgcgcc ggacagcctc gggccgctgg ccgactatgt gcggatcaag aacgcgcagg
     1741 ccgggtttcg cggccggatc gggctttatc ccggctcgcc catgctggcg ctcgatcacc
     1801 tgcgcgcggg cgacgcctgg ctcggctgcg aattgcggga ggacgatttc gaggcgttgc
     1861 gcgaacggct ggggcggcgc ggcgaggccg tgcgcaagga cggctacaag gtcgccgtgg
     1921 cgcaggcgca ggacgcgcgc cgcctgttgt acctgatcga tccgccgttc gagcgcccgg
     1981 acgattacga ccgcatcacc gccggcgtgc atgacattct gacgctgcgg cccgatgcgg
     2041 ttttcctgat ctggctgccg ctgaaagacc ttgagacctt cgattcctgg ctgcggcaga
     2101 tggagcgcga ggtgctggac gtcagcgagg cgtccctgct ggtcgccgag gtcaggctgc
     2161 ggccgctgac gaatccgatg aagatgaacg gctgcgcgct ggcctgcctg aacgcgccgg
     2221 aaggattcgc ggcgcaactg gcggccatcg ttgcggatgt ggcggcggtt ctgggcgagg
     2281 acggcgcgct ggggcggacg cggcttctgt aggcgcctgc gtcacatcgt cgtgaggacg
     2341 attctgcgcc ttggagtccg gcgatcaccg tgttatgctg cgttgcaata tggtccatcc
     2401 cccaaatctt cgaggccgag atgacgagcg cgaccgaaac ccccttctat ctgatgacgc
     2461 cgccggtcat gcagcctgag gacgtcagga aggcgtccga gcgcatgatg aacctgtggc
     2521 tgggcgcgat gtcgccgctg tgggcgccgt tctgggcggc gtcgagcttc ggcgtcggcg
     2581 tctgggccgt gtcgcataat ctgaagcctg ccgaggggct gctgacggaa ctgcccgccg
     2641 ccgacggttg gctgagcctg ttcaggtcat ggggcctcga agcgggcgaa ctgcacgaaa
     2701 aggcggtcga ggcggtcggg aacgccgccg aaaaggctga gtcgtccatc gaggcggcgt
     2761 ccgtcgaagc ggcgcccgcc gaggccgtgg cggtcgatcc cgtgaccgag gcgccggtcg
     2821 tgacgcctga ggcggcgccg gaacccgtcg cgccgaagcc gatcgcgcgc gcggtgtcgg
     2881 cggcgcgcaa gccccgcgcc aagacttaat tccgctgtaa ggtaaaggcg atcgggcgat
     2941 ggtccgatcc gatgtccggc ccgacgacgc ggccggacag gcgccagtcc gcgcccgcca
     3001 gcgcgttatc gatgctcagg cgcagcaggc cgggcagcgg cgacggccat gagccgttca
     3061 gcgcgggcag cgccgtcagg cggtagtccc gcgccagccc cttgagcata taggcgctca
     3121 tgtcgctgtt gaagtcgccg accagcaggg tcgcggcggg atcgcccgcg tcgaggcgct
     3181 ggcgcaggcg cgcgtcgaag ccggggcggc gcggctcgcg atgcgaatcg ggatggtcga
     3241 aatgcgcgcc gatcaggcgc agcggcccca gcggcgtccg catcgccgcc acgccgaaat
     3301 ggccgtccgg gtgacgctga taagggaagg gatagcgcgc gaaaatggcg atctcgccct
     3361 gctgattggc gtaaggataa tgcgcgtcca tccgcgcgcg caacatctcc cacaggcgcg
     3421 gggtcgtctc cgccgtgacg acgatgtcgg cccgctgcgc ctcggcccag gcggcgaggc
     3481 ggccgggatc gggattgcgg aaatagagat tggcgaaaac gacatgcaag gcgggcgcgc
     3541 cgtccttcgc cggcggccgc ggcggcagga tctgcggcgc cgcggcggcg aacaggacga
     3601 ggcaggccgc cagcgccacg accgccgcca gcctttgccg cagcagggcc agaaccgccg
     3661 tcagcgcgac gcaggcgctc agcagcggca cggtgaagat gtcgagcatg tagagccatg
     3721 tcggacgcgg atcgctcagg cagatcaggg cgacgatcag ggcggggacg gccaggctca
     3781 ggccgattac atgcagcgga cgcgaagccg gtcgggacat gggttgaaca tctgttgaca
     3841 ggagcgaggt caggccgcac ccttgcgccg gtcgcaacgg gaggcgggga atgcgggcgc
     3901 ggacgcacga ggacattctc aaaattcacg actcggtcga cacggtcaag cgcctgtccg
     3961 accgcatcat cggcatcggt ccgatcaatc tgatcgggct ggacggcatc ctgtcgtggg
     4021 ttccggttcc ggggctcgac ctcgcctatt cggtgctggc gggcgggttt atcctcgtgc
     4081 agggcgcgcg ggcgcgttgc gacgcgacca cgctggtcgc cgccgcgctg gtgctggcgg
     4141 tcgatgcggg gctgagcctc ggcgacacgg tgatcccggt gctgggcgcg gcggcggata
     4201 cgctgtttca ggggcagctc tatgcggcgc acatgattca gcgcaatatc gacaagacgc
     4261 actatgtcga aggcggcgaa gccgaggcgc gccgcaacgg cgaccatgcg cgccatgtcg
     4321 ccgacatgcg cgcgatgaag ggcaagacgc gggtcgtcta tctgggctga ataatcccca
     4381 tcgggcgcac cggcccgcag actgcatgga cattcgtttt cagtccatga ggtctttcgt
     4441 gagcgatcta acgccccgtc tttgtctgcc gctgatcggc gaccacgcgc agaagcagct
     4501 tgtcatgaac gccgcgctga tgcggctgga gagcctggtg caggcgcgcg tcgtcagccg
     4561 ctcgaccgcc gccgaaccgt ccgcgcccgc ggacggcgac gcctatatcc tgcccgccgg
     4621 ggccagcggc gcgacctggg gcgggctgag cgcgggcggc gtcgtgcgcg ccgagggcgg
     4681 cgt
//
LOCUS       DASVLL010000229         4855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_120618,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000229 DASVLL010000000
VERSION     DASVLL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120618"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF402_03745"
     CDS             <1..253
                     /locus_tag="VF402_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX7799436.1"
                     /translation="LLDAMSVYERAGAMAAIPLLGSVALALALWRGFLSGAASFAASR
                     VGEDFQAEQWGRDAEAEQRADALRAQAVSLETWFRALSY"
     gene            complement(366..698)
                     /locus_tag="VF402_03750"
     CDS             complement(366..698)
                     /locus_tag="VF402_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEX7799437.1"
                     /translation="MTERYTTYSAFFDFYLSEHSHPGTRALHYLGSTCGIAALILIVW
                     TRNPLWILAGLIAGYGCAWIGHAFIEHNRPATFKYPLWSFMGDYHMFFLWLTGQLGKR
                     RAAARRSV"
     gene            complement(853..2004)
                     /locus_tag="VF402_03755"
     CDS             complement(853..2004)
                     /locus_tag="VF402_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7799438.1"
                     /translation="MSPDMTADLALKLHSRIAEIGESAWNALRTDDNPFISYAFLEAL
                     EATGCVGDGTGWQPVHLSLSDASGPRGVMPLYVKTHSMGEYVFDQGWARAYEQAGGDY
                     YPKLLSAVPFTPVAGPRLMSPDPETRAALIRAAVGVCEANKLSSLHVNFPTQAEWQAM
                     GETGLLQRQDQQFWFDNPGYAGFDDFLADLSANRRKVIRRERRAVQSTVDLRWITGDD
                     LTPAHMDRLYAFISDTYERKWGTGRPYLTRAFFHRLLETMRMHIALVFAYADGEAVAG
                     AINFIGGDVLYGRQWGALIDIPFLHFEVCYYQAIDFAIARGLRRVEAGTQGEHKLSRG
                     YRPQPVYSAHYIRDPRLRAPVRRFLEAEREAVAEQMAMLSEAASPFRKG"
     gene            complement(2024..3415)
                     /locus_tag="VF402_03760"
     CDS             complement(2024..3415)
                     /locus_tag="VF402_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX7799439.1"
                     /translation="MTAAAATPVPQKEFLGHPVGLLYLVFAEAFERFSYYGMKTLLTL
                     YLVQQLLLPGHVEKVLGFVPFHHFLTGVYGPLSTVGLASVIVGLYGFTYLTPLLGGLI
                     ADRWLGRTTTITVGALIMTFGHFLMAFEQSFLIALVLILLGVGCFKGNISSQVGRLYG
                     PEDERRGSAFQIFYLVFNTAVIIAPIICGTLGQKVAWHWGFGAAGVSMLFGLAVYLLG
                     RRYLPPDEPKGAAARVEKTPLTGADWKKIILLLLMIPFIGLALVMNEQLGIGYLIWGN
                     DHLDLRFFGYDLPSTWLVSVDAFVSMITLSTSIAFWKWWSLKRKEPDELTKIAFSGIL
                     CALAPLALAAAAATTAHGHKASLGWVFLFEFLNDYGFSNLVPVAMALFVRVAPQQVNG
                     TMIGVFMLNFFLADFLAGALGEQLGPMGGMNFWLLHFGIVAFAVVALFLFWLIFGRTL
                     IPESGPHLRRQAH"
     gene            3442..3789
                     /locus_tag="VF402_03765"
     CDS             3442..3789
                     /locus_tag="VF402_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799440.1"
                     /translation="MTILGVKPGGCQARGPSSTARRELFDFSLELALIFISYNWHDRR
                     GAKMSLIARASGRCIRAAMLSGLSILALAAAAARAQDAPAQTSDQKADKADKDKATTT
                     VTVKGRNPASTID"
     gene            3838..>4855
                     /locus_tag="VF402_03770"
     CDS             3838..>4855
                     /locus_tag="VF402_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799441.1"
                     /translation="MDALNKVPGVVADSDGNVTYRGKAVAIYLNGHPSLMLQGDNRGI
                     ALQSMPSGYIGSIEVISNPGAQYGSAAGGGPILNIVTRHTLPPGGTVALMPKIRSDGG
                     ASLDGSIFYHVGKLTVMAFAGQTELRSSTHGDRSLQSFDATGALTGVTTSASDSRNRN
                     SAPHLFSNFEYKLTKDDVLNGQLMDFSGGGRSRSTSGVASYDGAGAPVNIYQSRSEGE
                     YTWGGPQLSLNWLHYGRLPDQTLKVNMQVSGGDNGSSGESRNAYQASSLPQNTGVRVT
                     RYDNKTDKRSGEFSLDYNTPVGDDQFATGVQVNLDSSHATNESYGPDDEGAAPTLNSL
                     LSRDF"
BASE COUNT          952 a         1530 c         1600 g          773 t
ORIGIN      
        1 gctgctcgac gccatgtcgg tctatgagcg cgccggggcg atggcggcga ttccgctgct
       61 cggttcggtg gcgctggcgc tggcgctgtg gcgcgggttc ctgtccggcg cggcgtcgtt
      121 cgcggcgtcg cgcgtcggcg aggactttca ggccgaacag tggggccgcg acgccgaggc
      181 cgagcagcgc gccgatgcgc ttcgcgcgca ggcggtcagt ctcgagacgt ggttccgggc
      241 tttgagttat tgaccacgga aagcacggaa aacacggact tcagggtgtg gccggcatct
      301 gcgcgataaa tcgtcatttc gctatgaggt ccgtgttttc cgtgttttcc gtggctaaat
      361 ctcttttaaa ccgatcgccg ggccgcggcc cggcgtttgc ctagctgacc tgtcagccac
      421 aggaagaaca tgtggtagtc gcccatgaac gaccacagcg ggtatttgaa ggtcgccggg
      481 cggttatgct cgataaaggc atgcccgatc cacgcgcagc cgtaaccggc gatcagaccg
      541 gccagaatcc ataacggatt gcgcgtccag acgatcagga tcaacgccgc gatcccgcag
      601 gtcgagccga gatagtgcag ggcgcgcgtg ccgggatggc tgtgttcgct caggtagaag
      661 tcgaagaagg ccgaataggt ggtgtagcgt tccgtcatgg cgcgcctcct gacggcatta
      721 tagcgtaaga gttggccacg aaacacacga aagaacacga aagcttcggc gccgccgaaa
      781 tattcgtgtt caggcgtggg cgttcgcagg acatagcgct tgcgcgcagg agtcctttcg
      841 tgtggttcgt ggctatccct tgcgaaacgg cgacgccgcc tcgctcagca tcgccatctg
      901 ctcggccacg gcttcgcgct cggcctccag aaagcgccgc accggcgcgc gcaggcgcgg
      961 gtcgcggata tagtgcgccg aatagaccgg ctggggccgg tagccgcgcg acagcttgtg
     1021 ctcgccctgc gtcccggcct cgacgcgcct caggccgcgc gcgatggcga agtcgatggc
     1081 ctgatagtag cagacctcga aatgcaggaa cggtatgtcg atcaaggcgc cccattgccg
     1141 cccgtacagc acgtcgccgc cgatgaagtt gatcgcgccc gcgaccgctt cgccgtccgc
     1201 ataggcgaag accagcgcga tatgcatgcg catcgtctcc agcaggcggt ggaagaaggc
     1261 gcgcgtcaga tagggacggc ccgtgcccca tttgcgctca taggtgtcgg agatgaaggc
     1321 gtagagccgg tccatatgcg ccggcgtcag gtcgtcgccg gtgatccagc gcagatcaac
     1381 ggttgactga accgcgcgcc gctcacgccg gatcaccttg cgccggttgg cggacaggtc
     1441 ggccaggaag tcgtcgaagc ccgcgtagcc cggattgtcg aaccagaact gctggtcctg
     1501 acgctgcaaa aggccggtct cgcccatcgc ctgccattcg gcctgcgtcg ggaagttgac
     1561 gtgcagcgag gacagcttgt tcgcctcgca gacgccgacg gcggcgcgga tcagggcggc
     1621 gcgcgtctca gggtcgggcg acatcaggcg cggcccggcg accggcgtga acggcacggc
     1681 gctcagcagc ttggggtaat agtcgccgcc cgcctgttcg taggcgcgcg cccagccctg
     1741 atcgaagacg tattcgccca tcgaatgggt cttgacgtag agcggcatga cgccgcgcgg
     1801 gccggaagcg tcgctcaggc tcagatggac cggctgccag cccgtgccgt cgccgacgca
     1861 acccgtcgct tccagagcct caagaaaggc ataggatata aaggggttgt cgtcggttct
     1921 taaagcgttc catgcggatt cgccgatctc agcgatgcgg ctgtgcagtt tcagggcgag
     1981 gtcggcggtc atgtctggtg acatagggcc gccgccgtcg aattcaatgg gcctgacgcc
     2041 tgagatgcgg tcccgattcc gggatcagcg tcctgccgaa gatcagccag aacaggaaca
     2101 gggccacgac ggcgaaggcg acgatgccga agtgcagcag ccagaagttc atgccgccca
     2161 tcggaccgag ctgctcgccc agcgctccgg ccagaaagtc ggccagaaag aagttgagca
     2221 tgaagacgcc gatcatcgtg ccgttgacct gctgcggcgc gacgcgcacg aacagggcca
     2281 tcgccacagg cacgaggttg gagaagccgt aatcgttgag aaactcgaac aggaacaccc
     2341 agccgagcga cgccttgtgc ccgtgggcgg tcgtcgcggc ggcggcggcc agagccagcg
     2401 gcgccagcgc gcacaggatg ccggaaaagg cgatcttggt cagttcgtcc ggctccttgc
     2461 gtttcagcga ccaccatttc cagaaggcga tcgaggtcga cagggtgatc atcgacacga
     2521 aggcgtccac cgacacaagc catgtcgagg gcaggtcgta gccgaaaaat ctcaggtcaa
     2581 gatgatcgtt gccccagatc aggtagccga tgccgagctg ctcgttcatc accagcgcca
     2641 gaccgatgaa cgggatcatc agcagcagca ggatgatctt cttccagtcc gcgccggtca
     2701 gcggcgtctt ttccaccctg gccgccgcgc ctttcggctc gtccggcggc aggtagcggc
     2761 ggcccagcag atagaccgcc aagccgaaca gcatggagac gcccgccgcg ccgaagcccc
     2821 agtgccacgc caccttctgc cccagcgtgc cgcagatgat cggcgcgatg atgacggcgg
     2881 tgttgaacac cagatagaag atctggaacg ccgagccccg ccgctcgtcc tccgggccgt
     2941 acaggcgtcc gacctgcgac gagatattgc ccttgaagca gccgacgccg agcaggatca
     3001 gcaccagcgc gatcaggaac gattgctcga acgccatcag gaaatggccg aaggtcatga
     3061 tcagggcgcc gacggtgatg gtcgtcgtgc gccccagcca gcggtcggcg atcaggccgc
     3121 ccagcagcgg cgtcaggtag gtgaagccgt aaagcccgac gatcaccgag gccagcccca
     3181 ccgtgctcag cgggccgtaa accccggtca ggaaatggtg gaacggaacg aagcccagca
     3241 ccttttcgac atggccgggc agcagaagct gctgcaccag atagagggtc agcagggttt
     3301 tcatgccgta ataggaaaac cgctcgaacg cctcggcgaa caccagatag agcaggccga
     3361 cggggtggcc gagaaactct ttctgcggga cgggcgttgc ggcggcggcg gtcatgcggg
     3421 catccatgaa aagttacgca agtgacgatc cttggcgtca agcccggcgg ctgtcaagcg
     3481 cgcggcccgt cttccaccgc aaggcgcgaa cttttcgatt tcagccttga attggccctg
     3541 atttttatct catacaattg gcatgatcga aggggggcga aaatgagtct tattgcgcgc
     3601 gcgtcgggac gatgcattcg ggccgcgatg ttgtcgggcc tgtccatcct tgcgctggcc
     3661 gccgccgcgg cgcgggcgca ggacgcccct gcgcagacat ccgatcagaa agccgacaag
     3721 gcggacaagg acaaggcgac caccacagtg acggtgaagg gccggaatcc tgcctcgacc
     3781 atcgactagc agatttatga cgttggcaag gatcccgaca gccagaccag cacggcggtg
     3841 gacgcgctga acaaggtgcc gggcgtcgtc gccgacagcg acggcaatgt cacctatcgc
     3901 ggcaaggcgg tcgccatcta cctgaacggc caccccagcc tgatgttgca gggcgacaac
     3961 cgggggatcg cgctgcaatc catgccgtcg ggctatatcg gctcgatcga ggtgatcagc
     4021 aatccgggcg cgcagtacgg ttcggcggcg ggcggcgggc cgatcctgaa catcgtcacg
     4081 cgccacaccc tgccgccggg cggcacggtc gccctgatgc cgaagattcg cagcgacggc
     4141 ggcgcctcgc tggacgggtc gatcttctat catgtcggca agctgaccgt gatggccttc
     4201 gcgggccaga ccgagcttcg cagctccacg cacggcgacc gaagcctgca aagcttcgac
     4261 gccacgggcg ccctgaccgg cgtgacgacg agcgcgagcg attcgcgcaa ccgcaattcg
     4321 gcgccgcatc tgttcagcaa tttcgaatac aagctgacca aggacgacgt gctgaacgga
     4381 caactgatgg atttcagcgg cggcggccgt tcccgctcca cgtccggcgt cgccagctat
     4441 gacggcgccg gggcgccggt gaatatctat caaagccggt ccgaaggcga atacacctgg
     4501 ggcgggccgc aactcagcct caactggctg cattacggcc gcctgccgga tcagaccctg
     4561 aaggtcaaca tgcaggtctc cggcggcgac aacgggtcca gcggggaaag ccgcaacgcc
     4621 tatcaggcgt ccagcctgcc gcagaacacc ggcgtgcgcg tcacgcgtta cgacaacaag
     4681 acggataaac gcagcggcga gttcagcctc gactacaaca cgccggtggg cgacgaccag
     4741 tttgcgaccg gcgtgcaggt caatctggat tccagccacg cgaccaacga aagctacggc
     4801 ccggacgacg aaggcgcggc gccgacgctc aacagcctgc tcagccgcga tttcc
//
LOCUS       DASVLL010000230         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_443947,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000230 DASVLL010000000
VERSION     DASVLL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443947"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF402_03775"
     CDS             complement(<1..215)
                     /locus_tag="VF402_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799442.1"
                     /translation="MAATGASNNNNLVVILAVALIAIVAVAAVYLMQDHRGPAERAGD
                     AVAALPNGLGKAANKLDDQPPAQNVKR"
     gene            complement(309..941)
                     /locus_tag="VF402_03780"
     CDS             complement(309..941)
                     /locus_tag="VF402_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEX7799443.1"
                     /translation="MLFTTYLHYFWADHAYLRLGFTNAHWIGDKMVRTNQPWPFQLKW
                     WRDKGVKTVINLRGGKGSFYYLEKHACEKLGLRLEDFGLTSRSLPTGAEILAARALFD
                     RIEYPALLHCKSGADRAGIMSVLYRHLHLGHPIREAAQELGLRTLHMKAGMTGVLDYL
                     FEVYLRDIEPQGIGFLDWVTGPDYDPDRLKAEFKGAWWGKLLTDRLLRRE"
     gene            1120..2946
                     /locus_tag="VF402_03785"
     CDS             1120..2946
                     /locus_tag="VF402_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7799444.1"
                     /translation="MPLSETPPASLSRTALLARVWRDYLSPQKARFGVALICAVVVAA
                     TTSLLLGGLNPAVNLLLHVPAPDAVRLPGWLTAHPVTIIPLFFIAAALARFVAQRGLT
                     LSLNHVGNTLVGRIQSQLFSRLVHADLGRLQKSHSGEYLSSVLYDADLVREAASASAV
                     SYLQNLLIAAGAFFAMFHADWTMALSVLVLGPFLSLVMGRYLKLTRKAAKAAMAETSS
                     LSTDIMESLDGIRIVKIANQEAFEEQRVGAVIERRQAHIIKGSNARTMAAPATELLTG
                     LLLAGLLAYVGWRSLHGGMTAGALLSFLAALGVAGQALRQLAGLQGAFSQGMTAARRL
                     FAALDIRPEIVSAPGAAELPAHIGPIRFIEARFAYGEAPVLDGVSFTVEPGQSVALVG
                     PSGSGKSTLLNLIPRFFDLTSGDIRFGDVSTRAVTLPSLRARIALVTQDPFLFDDTIR
                     ANIAYGNGAPTQAEVEQAAKDAAAHDFITALPQGYDTRAGEAGSRLSGGQKQRIAIAR
                     AFLKNAPILLLDEATSALDTESEQRVQEALERLMRGRTTFVVAHRLSTVRHADQILVL
                     DHGRIAERGTHDALIRQGGLYARLAAAQFDLPAETATGEAAR"
     gene            2943..3671
                     /locus_tag="VF402_03790"
     CDS             2943..3671
                     /locus_tag="VF402_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7799445.1"
                     /translation="MKSLLDSVLFRRLIASSVSGYLKFCYATTRWTYEGRAEVERVWA
                     EGGPVILMFWHERLHLGHAGWPYPGLAKDRAQPVAVLTSLNKFGDVIELINSYFEHHT
                     IRGSTAKKSDPAKNKGGAQAFRDLLRWVRKGACAATTPDGPRGPRRYLQEGSLKLSQM
                     CDAKLVCLGQATHNHLRFNSWDRMRLPLPFGKGAMVWRVLPAVPKDAGEAELERLRRE
                     AEAALNAVTDRADALAGLTPEPAA"
BASE COUNT          589 a         1290 c         1239 g          612 t
ORIGIN      
        1 ttgcgcttga cgttctgggc gggcggctgg tcgtcgagct tgttggcggc cttgcccagc
       61 ccgttcggca gggcggcgac cgcgtcgccc gcgcgctcgg cggggccgcg atggtcctgc
      121 atcaggtaaa cggcggcgac cgcgacgatg gcgatcagcg ccacggccag aatgacgacc
      181 agattgttgt tattggatgc gcctgtagcg gccatgacga actcccggat tgctgtctgt
      241 gatccagacc tagcagaccg ggcgcaattt tgcggtaacg gcctgacgcc cgccccgtaa
      301 ggatttcatt attcccgccg cagcaacctg tcggtgagca gcttgcccca ccacgcgccc
      361 ttgaactcgg ccttgagcct gtccgggtcg tagtccgggc cggtcaccca gtcgaggaag
      421 ccgatcccct gcggctcgat atcgcgcagg taaacctcga acagatagtc cagcacgccg
      481 gtcatgcccg ccttcatgtg cagggtgcgc aagcccagtt cctgcgccgc ctcgcggatc
      541 ggatggccca gatgcaggtg gcggtaaagc acgctcatga tccccgcccg gtccgcgccc
      601 gacttgcagt gcaaaagcgc cggatattcg atgcggtcga acagcgccct ggcggccaga
      661 atctccgccc ccgtcggcag cgagcgcgag gtcaggccga aatcctccag ccgcagcccc
      721 agcttttcgc aggcgtgctt ttcgagataa tagaacgacc ccttgccgcc gcgcaggttg
      781 atcaccgtct tcacgccctt gtcgcgccac cacttaagct ggaacggcca cggctggttc
      841 gtgcgcacca tcttgtcgcc gatccagtgc gcgttggtga agccgagccg caggtaagca
      901 tggtcggccc agaaataatg cagataggtg gtgaaaagca tccacggcgt atcggtgcgg
      961 aacggctgcg tatggacttt ctttttcagc agtttcaaac ggaaatccga tatggcgaag
     1021 gcgtgtgcgc ggcgcgccgt taacgcataa agacgcgaca ggcgcaatga aatgcctata
     1081 taacggctgc cacgcttttt gccatagcca agacgactta tgccgctttc cgaaacgccg
     1141 cccgcctcat taagccgcac cgcgctgctg gcccgcgtct ggcgcgatta cctcagtccg
     1201 cagaaggcgc gcttcggcgt ggccctgatc tgcgccgtcg tcgtggcggc gacgacctcg
     1261 ctgctgctcg gcgggctgaa cccggcggtc aacctgctgc tgcatgttcc ggcgcccgac
     1321 gcggtcaggc tgccgggctg gctgaccgcg catccggtca cgatcattcc gctgttcttc
     1381 atcgcggcgg cgctggcgcg cttcgtggcg cagcgcggcc tgaccctgtc gctgaaccat
     1441 gtcggcaaca cgctggtcgg caggatccag tcgcaactgt tctcgcgcct cgtccacgcc
     1501 gatctggggc ggctgcaaaa atcgcactcc ggcgaatatc tgtcgtcggt gctgtacgac
     1561 gccgacctcg tgcgcgaagc cgcctcggcc agcgcggtgt cctatctgca aaacctgctg
     1621 atcgcggcgg gggcgttttt cgccatgttc cacgccgact ggacgatggc gctgagcgtg
     1681 ctggtcctgg ggccgttcct gtcgctggtc atgggccgct acctgaagct gacgcgcaag
     1741 gcggccaagg cggcgatggc cgagacgtcc agcctgtcca ccgacatcat ggagagcctc
     1801 gacggcatcc gcatcgtcaa gatcgccaat caggaagcgt tcgaggaaca aagggtcggc
     1861 gcggtgatcg agcgcaggca ggcgcatatc atcaagggct cgaacgcgcg caccatggcc
     1921 gcgcccgcca ccgagctgct gaccgggctg ctgctggccg ggcttttggc ctatgtcggc
     1981 tggcgcagcc tgcacggcgg catgacggcg ggcgccctgc tctcgttcct ggccgctctg
     2041 ggcgtggcgg ggcaggcgct gcggcaactg gccggattgc agggcgcgtt cagccagggc
     2101 atgaccgccg cgcgccgcct gttcgccgcg ctcgacatcc gcccggagat cgtcagcgcg
     2161 cccggcgccg ccgaactgcc cgcgcatatc ggcccgatcc gcttcatcga ggcgcgcttc
     2221 gcctatggcg aggcgccggt gctggacggg gtgagcttca ccgtcgagcc gggccagagc
     2281 gtggcccttg tcgggccgtc gggatcgggc aagtcgaccc tgctcaacct gatcccgcgc
     2341 tttttcgacc tgacctccgg cgacatccgc ttcggcgacg tttcgacgcg cgccgtcacc
     2401 ctgccgtcgc tgcgcgcgcg catcgcgctg gtgacgcagg atccgttcct gttcgacgat
     2461 acgatccgcg ccaatatcgc ctacggcaac ggcgcgccca cgcaggccga ggtcgaacag
     2521 gccgcgaagg acgccgccgc gcacgacttc atcacagccc tgccgcaggg ttacgacacg
     2581 cgcgcgggcg aagccggatc gcgcctgtcg ggcggccaga agcagcgcat cgccatcgcg
     2641 cgcgccttcc tgaaaaacgc gccgatcctg ctgctcgacg aagcgacctc ggcgctcgac
     2701 accgaatcgg aacagcgcgt gcaggaggcg ctggagcggc tgatgcgcgg gcgcaccacc
     2761 ttcgtggtcg cgcaccggct gtcgaccgtg cgccacgccg accagattct ggtgctggac
     2821 cacggccgga tcgccgagcg cggaacgcac gacgcgctga tccgccaggg cggcctctac
     2881 gcccgcctcg ccgccgccca gttcgacctg cccgccgaga ccgcgaccgg ggaggccgcg
     2941 cgatgaagag cctgctcgac agcgtcctgt tccgccgcct gatcgcctcc agcgtgtccg
     3001 gctacctgaa attctgctac gcgacgacgc gctggaccta tgagggccgg gccgaggtcg
     3061 aacgggtctg ggccgaaggc gggccggtga tcctgatgtt ctggcacgag cggctgcatc
     3121 tgggccatgc gggctggccg tatccggggc tggcgaagga ccgcgcccag ccggtggccg
     3181 tgctgacctc gctcaacaag ttcggcgacg tgatcgagtt gatcaacagc tatttcgagc
     3241 accacaccat tcgcggctcg accgccaaga agtccgaccc ggccaagaac aagggcggcg
     3301 cgcaggcgtt ccgcgacctg ctgcgctggg tgcgcaaggg cgcctgcgcc gccaccacgc
     3361 cggacgggcc gcgcgggccg cgccgttatc tgcaagaggg ttcgctcaag ctgtcgcaga
     3421 tgtgcgacgc gaagctggtc tgcctgggtc aggcgacgca caaccatctg cgcttcaact
     3481 catgggaccg gatgcgcctg cccctgccct tcggcaaggg cgcgatggtg tggcgcgtgc
     3541 tgcccgccgt gccgaaggac gccggcgagg ccgaactgga gcgcctgcgc cgcgaggccg
     3601 aggcggcgct caacgccgtc accgaccgcg ccgacgcgct ggccgggctg acgccggagc
     3661 cggcggcgtg acggcggcga aagacacgcc ggccctggcc ctttatcgtc aggcgatgcg
     3721 cgcctttcac
//
LOCUS       DASVLL010000231         4399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_335777,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000231 DASVLL010000000
VERSION     DASVLL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335777"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VF402_03795"
     CDS             <1..1132
                     /locus_tag="VF402_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEX7799446.1"
                     /translation="FTLLGLYADGEDGLARGMIGDFESLIERYGHIPNGARSYYLSRS
                     QPPFFALMTELKPADDPDPARTLKALRAEHDYWMKGASCAVVMKPCRNVVRMPDGGLL
                     NRYWDARDTPREESYAEDVATAAKSARPQADVYRDLRAAAESGWDFSSRWLRDPMKLE
                     TIHATDIVPVDLNSLLWKDETIIAARCEAAGDADCARDYKAMAQARKAEITRWLWRAA
                     QHRYADYDERARRPTPVISAATLYPLFVGLADPRQAAAVATLTRLQLTAPGGLRATQI
                     ASGQQWDDPNGWPPLQWIAMSGLDAYGFHKQARDLAARFVGTVDAAYRGTGKMLEKYD
                     VEQRRPGGGGEYPLQDGFGWTNGVTRAILAKYPDLDPEGDAE"
     gene            1179..1430
                     /locus_tag="VF402_03800"
     CDS             1179..1430
                     /locus_tag="VF402_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX7799447.1"
                     /translation="MARTTSVSLGDHFSSFIAEKVETGRYGSSTDVIRAGLRLLEERE
                     ARIENLRQALAAGEASGEPRRFDADAFLKRMRAQHDDAV"
     gene            1414..1713
                     /locus_tag="VF402_03805"
     CDS             1414..1713
                     /locus_tag="VF402_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7799448.1"
                     /translation="MTTQFDGYRLRPQAEADLETIWRYSARRWSIRQAEIYIGEIMTA
                     LEDLALGRCYGRRKEEVPDYLNYAVGSHNIWFRTAGFIDVVRILHQSMDVDAYLF"
     gene            complement(1884..2585)
                     /locus_tag="VF402_03810"
     CDS             complement(1884..2585)
                     /locus_tag="VF402_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HEX7799449.1"
                     /translation="MFTEPVIDLTVHHQPRGVSDRFAYGFTKLLRFCADTFFARRYGH
                     RAIVLETVAAVPGMVGATITHLKCLRRMKDDKGWIRTLMEEAENERMHLMTFIEVAKP
                     TFFERFVIIAVQWVFYLAFFALYLISSRTAHRVVGYFEEEAVISYTHYLKEIDEGRSP
                     NIPAPAIARRYWKLPETATLRDVVLMVRADEAHHRDVNHAFSNELKGGAADLAAEVAP
                     YPAHDESLCVKKIAA"
     gene            complement(2717..3493)
                     /locus_tag="VF402_03815"
     CDS             complement(2717..3493)
                     /locus_tag="VF402_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7799450.1"
                     /translation="MKPDNLDRIRRLQLFAGMSGDSFTQVTRGALMQKYPAETVLLYE
                     ADAVDFLYIVLDGSVELHARWHHHETTLAVLRPVSTFILAAVVLEAPALMSARTLERA
                     EIVMIPAETLREAMRTDAGFAFAVAQELSGCYRGLVRTIKNHKLRSGTERLANYLLTQ
                     QVRQGGPERIALPHEKRILAALLDLTPESLSRAFAALARYGVAVQGANVTIGDAAALR
                     RMARPDALIDNHMPPRAHKEGKAAKEAWPPFERSCSEYRD"
     gene            3572..>4399
                     /locus_tag="VF402_03820"
     CDS             3572..>4399
                     /locus_tag="VF402_03820"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7799451.1"
                     /translation="MPMFRSILPVAVLLAAAPVHAEILRADARAQAPAPVSAQLHMGT
                     AAGPNGPITVNNRYLMKDGQPWLPVMGEMHFTRLPAGEWDAELQKMKAAGVDIVAAYV
                     IWSHHEEKPGVYDFSGDRDIRRFLDLAKKNGLYVVLRLGPWDHAEVRFGGIPDWVVAQ
                     VPTRGDDPQYLAYVRDYWSHLAGQTRGEMYKDGGPVIGVQLENEYNLTGPGRGAEHIA
                     TLKRMARDLGFDVPLYTVTGWDGAVYPKGEVLPVQGGYPDTPWGTSPKMSPPNEVYAF
                     "
BASE COUNT          739 a         1326 c         1560 g          774 t
ORIGIN      
        1 tttcaccctg ctgggcctct atgccgacgg cgaggacggg ctggcgcgcg gcatgatcgg
       61 cgatttcgag agcctgatcg aacgctacgg ccatatcccc aacggcgcgc gcagctatta
      121 tctcagccgc tcgcagccgc cgtttttcgc gctgatgacc gaactgaaac cggcggacga
      181 tcccgatccg gcgcgcacgc tgaaagccct gcgcgccgaa cacgattact ggatgaaggg
      241 cgcgtcctgc gcggtcgtga tgaagccgtg ccggaacgtg gtcaggatgc cggacggcgg
      301 gcttctgaac cgctactggg acgcgcgcga tacgccgcgc gaggagtcct acgccgagga
      361 cgtggcgacg gcggcgaagt ccgcgcgtcc acaggcggac gtgtatcgcg acctgcgcgc
      421 ggcggcggaa agcggctggg acttttcctc gcgctggctg cgcgatccga tgaagctgga
      481 gacgatccac gccacggaca tcgttccggt cgatctcaac agcctgttgt ggaaggacga
      541 gacgatcatc gccgcgcgct gcgaggcggc gggggacgct gactgcgcgc gcgattacaa
      601 ggcgatggcg caggcgcgca aggcggagat cacgcgctgg ctgtggcggg cggcgcagca
      661 tcgctacgcc gattatgacg aacgcgcccg gcgtccgacc ccggtgattt ccgccgcgac
      721 gctttatccc ttgttcgtcg ggctggccga tccgcggcag gcggcggcgg tggcgacctt
      781 gacgcggctg caactgaccg cgccgggcgg gctgcgcgcc acgcagatcg cgtcggggca
      841 gcaatgggac gatccgaacg gctggccgcc gctgcaatgg atcgcgatgt ccgggctgga
      901 cgcctacggc ttccacaagc aggcgcgcga tctggccgcg cgcttcgtcg gcacggtcga
      961 cgcggcctat cgcgggaccg gcaagatgct ggagaagtac gatgtcgagc agcgcaggcc
     1021 cggcggcggc ggcgaatacc cgttgcagga cggcttcggc tggaccaacg gcgtgacccg
     1081 tgcgattctg gcgaaatatc ccgatctcga cccggagggg gatgccgaat agcacattat
     1141 gtgctaatat tcagcacaac atgaatcagg atattgttat ggcgcgcaca acgtcggttt
     1201 cgctcggaga ccatttctcc agtttcattg ctgaaaaagt cgagacgggt cgttatggct
     1261 cgtcaaccga cgtgatccgg gcgggattgc gtttgctgga agagcgagag gccagaatcg
     1321 agaatttgcg acaggccttg gcggcgggcg aagcttcggg cgagccgaga cgcttcgacg
     1381 ctgacgcttt cctgaagcgg atgcgcgcgc aacatgacga cgcagtttga cggctatcgt
     1441 ctgcgtccgc aggccgaagc ggatttggag acgatatggc gttacagcgc aaggcgctgg
     1501 tcgattcggc aggcggagat ttatatcggt gagataatga cggcgctgga agatttggcg
     1561 ttggggcggt gttatggtcg tcggaaagag gaagtccccg actatctgaa ctatgccgtc
     1621 ggctcgcata atatttggtt ccgtacagca ggttttatcg atgtcgtgcg tattctgcat
     1681 cagagcatgg atgtggatgc gtacttgttt tagacttttg cctgtcttct gacagcgcca
     1741 gcctgcgggc gaaggcgatg agggcggcgg aggttatgcc tccgccgccg tcgacgcgcc
     1801 acaggccttc gccgtcggat tcgagcctgt ggccggccgc ctgcaccgcg acgatcgcct
     1861 tgctgaccga ggcggcgcgc atgtcaggcc gcgatcttct tcacgcacag gctttcgtcg
     1921 tgcgcggggt agggggcgac ttcggcggcg aggtcggccg cgccgccttt cagctcgttc
     1981 gagaaggcgt ggttgacgtc gcggtgatgg gcctcgtcgg cgcgcaccat cagcaccacg
     2041 tcgcgcaagg tggcggtttc gggcagcttc cagtagcgcc gggcgatggc cggggccggg
     2101 atgttcgggc tgcggccctc gtcgatctcc ttcaggtaat gggtgtagct gatcacggcc
     2161 tcctcctcga aatagccgac gacgcggtgc gcggttctgg acgagatcag gtacagggcg
     2221 aagaaggcga ggtagaagac ccactgcacg gcgatgatga cgaagcgctc gaagaaggtc
     2281 ggtttggcga cctcgatgaa ggtcatcagg tgcatccgct cgttttcggc ctcctccatc
     2341 agggtgcgga tccagccctt gtcgtccttc atgcggcgca ggcacttcag gtgggtgatg
     2401 gtcgccccga ccatgcccgg cacggcggcg accgtctcca gcacgatggc gcggtggccg
     2461 tagcgcctgg cgaagaaggt gtcggcgcag aagcgcagca gcttggtgaa gccgtaggcg
     2521 aagcggtcgg aaacgcccct gggctgatgg tggacggtga gatcgatgac gggttcggtg
     2581 aacatggacg ctggccttta cggatgcttg aacatgtccg caggtgtagc gcgcggcgcg
     2641 gcggtgcgcc ttaattacgg gtaagcgggc gcttgagcgt gcgcaagcgg cggcttgata
     2701 tatgtcaagc ggcggctcag tcgcggtatt cgctgcacga gcgctcgaac ggcggccacg
     2761 cttccttggc ggccttgcct tccttgtgcg cgcgcggcgg catgtggttg tcgatcagcg
     2821 cgtcgggccg ggccatgcgg cgcagcgcgg cggcgtcgcc gatggtgacg ttcgcgccct
     2881 gcacggcgac gccgtagcgg gccagggcgg cgaaggcgcg cgacaggctt tccggcgtca
     2941 ggtccagcag cgcggccagg atgcgctttt cgtgcggcag cgcgatgcgc tcaggcccgc
     3001 cctgacgcac ctgctgggtc aggagatagt tggccagccg ctcggtgcct gagcgcagct
     3061 tgtggttctt gatggtgcgc acgaggccgc gatagcagcc ggacagttcc tgcgccacgg
     3121 cgaaggcgaa gcccgcgtcg gtccgcatcg cctcgcgcag cgtttcggcg gggatcatca
     3181 cgatctcggc gcgctccagc gtccgcgccg acatcagggc cggggcctcc agcacgacgg
     3241 cggccagaat gaaggtcgag accggccgca acaccgccag cgtcgtctcg tggtgatgcc
     3301 agcgcgcgtg cagttcgacg ctgccgtcca gcacgatata gaggaaatcg accgcgtcgg
     3361 cctcgtacag cagcaccgtc tccgccgggt atttctgcat cagcgcgccg cgcgtcacct
     3421 gcgtgaagct gtccccggac atgcccgcga acagttgcag gcggcggatg cggtcgagat
     3481 tgtctggctt catgacgcga ctctatacgc ttggcttgcg cgcgggaagc ggctattgaa
     3541 ttgtcataca tataacagga aatgccgtcc catgcccatg ttccgctcga tcctgccggt
     3601 cgccgtcctg ctggcggcgg cgcccgttca tgccgaaatc ctgcgcgccg acgcccgcgc
     3661 gcaggcgccc gcgcccgtat ccgcgcaact gcatatggga acggcggcgg ggccaaacgg
     3721 gccgattacg gtcaacaacc gctacctgat gaaggacggc cagccgtggc tgccggtgat
     3781 gggcgagatg cacttcacgc gcctgcccgc cggcgagtgg gacgccgagt tgcagaagat
     3841 gaaggcggcg ggcgtcgata tcgtcgcggc ctatgtcatc tggagccacc atgaggaaaa
     3901 gccgggcgtc tatgatttca gcggcgaccg cgacatccgg cgcttcctcg atctggcgaa
     3961 gaagaacggg ctttacgtcg tgctgcggct ggggccgtgg gaccatgccg aagtgcgctt
     4021 cggcggcatt cccgactggg tggtcgcgca ggttccgacg cgcggcgacg atccgcagta
     4081 tctggcctat gtgcgcgatt actggagcca tctggccgga cagacgcgcg gcgagatgta
     4141 caaggacggc ggaccggtca tcggcgtgca gctggagaac gaatacaacc tgaccgggcc
     4201 ggggcggggg gctgagcata tcgcgaccct gaaacgcatg gcgcgcgatc tcggcttcga
     4261 cgttccgctc tacacggtga ccggctggga cggggcggtc tatccgaagg gcgaggtctt
     4321 gccggtgcag ggcggctatc ccgacacgcc gtggggaacg tcgccgaaga tgtcgccgcc
     4381 gaacgaggtt tacgccttc
//
LOCUS       DASVLL010000232         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_227634,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000232 DASVLL010000000
VERSION     DASVLL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227634"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1086)
                     /locus_tag="VF402_03825"
     CDS             complement(97..1086)
                     /locus_tag="VF402_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX7799452.1"
                     /translation="MKAISVHNNGEKPDDLYPVDAPAPEVPDGHVLIRTRFAGVNRPD
                     VLQRRGLYPPPPGASPILGLEVAGVIAQGAPGWPEGTEVCALVNGGGYAEYVSVDARH
                     LLPVPKGLTLAEAASLPENVLTVYANLIEHGGLRPGETVLVHGGNSGIGAMTIQIAKA
                     IFNDKGGAYVIATARGAAKCAYARRSGADLVIDSAAGDFVAPVKAAGGADVALDIVGG
                     DFVAKNLLCLNMRGRIVQVGFGKSAVVEIDLRRVIAKQAVLTGSMLRPRSADEKARLT
                     RAVRERVWPLIESGMIRPILSQTVPFAQAAEAHRWLEAGDHCGKAVLELPTGP"
     gene            1207..1416
                     /locus_tag="VF402_03830"
     CDS             1207..1416
                     /locus_tag="VF402_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX7799453.1"
                     /translation="MVEDDLPARIGRDSALFAVAREDLDPYSVEDLRLRIEMLEAEIA
                     RSRAAMDSKTHRRSAADALFSFKGS"
     gene            1546..2058
                     /locus_tag="VF402_03835"
     CDS             1546..2058
                     /locus_tag="VF402_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX7799454.1"
                     /translation="MSDSNSSPLSARVHLVRDFAASDLFDRTFREGMALVEETAAYLD
                     GDGRRDSRLLSREDALLYAGESMRLTTRLMQIASWLLVQRAVREGDMEAQDACDDKYR
                     LAPVRGGEAGEAVTLPEGLLNLLDRSNRLYERISHMDRRMFAEGEVVEADINPVMSQL
                     QMLQTAFGGE"
     gene            2192..>2493
                     /locus_tag="VF402_03840"
     CDS             2192..>2493
                     /locus_tag="VF402_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7799455.1"
                     /translation="MFFRLRGLGAFLLWLVATPALCATAAQTAFPWAVSDEPLDPAIH
                     AGRLSNGLSYIIVHNATPSGTATVRMRVAVGSLMESPGEQGIAHYLEHMAFNGSTH"
BASE COUNT          403 a          876 c          795 g          419 t
ORIGIN      
        1 ccgccgtgtc gggcgaggag tcgggcctca acggcttcat gatcagggac gggcgctacg
       61 acgccggcac ggacccgcgc cgcgaaggcg tggcgctcac gggccggtag gcagctccag
      121 caccgccttg ccgcagtgat cgcccgcctc cagccagcgg tgcgcctcgg cggcctgcgc
      181 gaacggcacg gtctggctca ggatcggccg gatcatcccg ctttcgatca gcggccagac
      241 gcgctcgcgc accgcccgcg tcagacgcgc cttctcgtcc gccgaacgcg ggcgcagcat
      301 cgagccggtc aggaccgcct gcttcgcgat cacccggcgc aggtcgatct cgaccacggc
      361 gcttttgccg aagccgacct gcacgatgcg gccccgcatg ttcaggcaca gcaggttctt
      421 cgcgacgaaa tcgccgccga cgatgtcgag cgcgacgtcg gcgccgcccg ccgccttgac
      481 cggcgcgacg aagtctccgg cggcgctatc gatcacgagg tcggcgcctg acctgcgcgc
      541 ataggcgcat tttgccgccc cgcgcgccgt cgctatgaca tatgcgccgc ccttatcgtt
      601 aaaaatggct ttcgcgatct ggatggtcat cgccccgatg ccggaattgc cgccgtggac
      661 cagcacggtt tcccccggcc gcaagccgcc atgctcgatc aggttggcgt acacggtcag
      721 cacgttttcc ggcagcgacg ccgcctcggc cagcgtcagc cccttcggaa ccggcagcag
      781 gtggcgcgcg tcgacgctga cgtattccgc atagccgccg ccgttgacca gcgcgcagac
      841 ctccgttccc tccggccatc cgggcgcccc ctgcgcgatc acgcccgcga cctccagccc
      901 cagaatcggc gaggcgcccg gcggcggcgg ataaagcccg cgccgttgca aaacgtcggg
      961 ccggttcacg cccgcaaagc gcgtgcggat caggacatgc ccgtcgggaa cctccggcgc
     1021 cggtgcgtca accggataca ggtcgtcggg tttttccccg ttattatgaa cagagatggc
     1081 cttcatgccg attcgttttc cttgatttct tcgcgcgaca ggcgtttcat gacgccccgg
     1141 accatgggcc gttcctgcgg cccgtgtcca gccctgaagc gaggtcatga aagggagatt
     1201 tgggggatgg tggaagacga cctgcccgcc agaatcggtc gcgacagcgc gcttttcgcc
     1261 gtggcgcgcg aggatctgga cccttacagc gtcgaggatc tgcgcctgcg catcgagatg
     1321 ctggaagccg agatcgcccg cagccgcgcc gcgatggact ccaagaccca ccggcgctcc
     1381 gccgccgacg cgcttttctc ctttaagggt agttaagcct ttctaaccat aatgacgcct
     1441 catgactggc acaggcgttg cagacgcctt tccgaggttt tcacgatgac acgcaacagg
     1501 cgggtccaag gcgaaacctg accgatttag cacaaggccc gtttgatgtc cgacagcaat
     1561 tcctccccgc tcagcgcccg cgtgcatctg gtgcgcgatt tcgccgcctc cgacctgttc
     1621 gaccgcacct tccgcgaagg catggcgctg gtggaggaaa ccgccgccta tctcgacggc
     1681 gacgggcgcc gcgactcgcg ccttctgagc cgcgaggacg cgctgctcta tgccggggaa
     1741 agcatgcgcc tgaccacgcg cctgatgcag atcgcctcgt ggctgctggt gcagcgcgct
     1801 gtgcgcgaag gcgacatgga ggcgcaggac gcctgcgacg acaagtaccg cctggcgccg
     1861 gtgcgcggcg gcgaagccgg cgaagcggtg accctgcccg agggcctgct gaacctgctc
     1921 gaccgctcca accgcctcta tgaacgcatc tcgcacatgg accgccgcat gttcgccgag
     1981 ggcgaggtgg tcgaagccga catcaacccg gtcatgagcc agctgcaaat gctgcaaacc
     2041 gccttcggcg gcgaataggc ccgcggcggc cgacaagcgg ctgccgcttt ccacccgaaa
     2101 cctgttgaca ggattccgcc aaggctccag cctgacgcgg agcatgtccg cccgaggcca
     2161 tgcgcctgag gggattttgg ggggaatttt catgtttttt cggcttcggg ggctgggcgc
     2221 cttcctgttg tggctggtcg cgacgcccgc attgtgcgcg acggcggcgc agacggcgtt
     2281 tccctgggcg gtcagcgacg agccgctgga ccccgccatc cacgccggac gcctgtccaa
     2341 cggcctgagc tacatcatcg tccataacgc cacgccgtcc ggcacggcga cggtccgtat
     2401 gcgcgtcgcc gtcggctcgc tgatggaatc tccgggcgag caaggcatcg cgcattatct
     2461 ggagcatatg gccttcaacg gctcgactca tat
//
LOCUS       DASVLL010000233         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_66957, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000233 DASVLL010000000
VERSION     DASVLL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66957"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VF402_03845"
     CDS             <1..982
                     /locus_tag="VF402_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX7799456.1"
                     /translation="LREQADQIQKLSHQNGQVDLADLNAADSGAPLDAGAMSIAERFS
                     RFDHQGADNARQAQAMNALRTTSYIRGEKAQALQASLQTSLRGALIDDAQAASVSDHP
                     VAARAASAFKFANHNQNDLDCLSQAVYYEARGEGQDGMRAVAQVIVNRVRNPNYPHSI
                     CAVVYQGASNGGSCQFSFACDDAIGAPVERSAWRRARAVARAALDGYVMTGVGTATSF
                     HTVSVHPGWSATMQRVATIGSHVFYTFRGRSTQLNGVVRPSDTPAPVTVAERSDDNAR
                     TALLNALARDAGAEKAARPVAASAQIEGRAAHDIAAAVEKPAKAPAPAAS"
     gene            1234..2952
                     /locus_tag="VF402_03850"
     CDS             1234..2952
                     /locus_tag="VF402_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7799457.1"
                     /translation="MLKPSSTAGGLKISNWSFTIKFMAPAAIATAMIAVLTIGAVMVM
                     NGQGQTIDRINNVVMPQVQELGDVKAAIKEDNGQLFRALTLKATDPTANASSKVNAVA
                     TDLGKIADRVKKDVDMTKDPAQKKLLTDLGKEVKTYKEAVEFAGSVMDVDFTSVPPLM
                     SQFDDGYAKMSALSDQLITANVAAAKKAAADAKAAQATGISLLTAFAVIMAVVSGAIA
                     FLFSRVTVTGINRIAKTTEELAKGNLDVDISTLARRDELKSVVDSLNVFKSNAEEKQR
                     LMAIEAATAKTREERARQMSELAERFRHEAQDMLDALSSAASDLDANGRTLLTIAQEN
                     ERRSQDAVGSIRNSADNVQNVASATTELSASIGVIGDQAVRSVEIAAEAVSEADRTNE
                     SMAELSRAADQIGEVVDLINAIAQQTNLLALNATIESARAGEAGKGFAVVASEVKSLA
                     QQTAKATDEIRDRIKDIQAAAQNGVGAIRGIGETIKHMNEIAASIADSVHQQGDATNE
                     IARNVNEASDGTSMASSSVAQLSASAADTEKASTEMLGAANQLTQRTQAMSENIRRFL
                     SELTAA"
BASE COUNT          572 a         1077 c          895 g          414 t
ORIGIN      
        1 ccttcgtgaa caggcggacc agattcagaa gctgtcccat cagaacggac aggtcgatct
       61 ggccgatctc aacgccgccg acagcggcgc gccgctcgac gcgggcgcga tgagcatcgc
      121 cgaacgattt tcgcgtttcg accatcaggg cgccgacaac gcccgccagg cgcaagcgat
      181 gaatgcgctg cgcaccacca gctatatccg cggcgaaaag gcgcaggctt tgcaagcctc
      241 gttgcagacc tcgctgcgcg gcgcgttgat cgacgacgcg caggccgcct cggtttccga
      301 ccatccggtc gccgcgcgcg ccgcctcggc gttcaagttc gccaaccata accagaacga
      361 tctcgactgc ctgagtcagg ccgtctatta cgaagcgcgc ggcgaaggcc aggacggcat
      421 gcgcgccgtg gcccaggtca tcgtgaaccg cgtgcgcaat cccaactatc cgcacagcat
      481 ctgcgccgtc gtctatcagg gcgcgtcgaa cggcggttcg tgccagttca gcttcgcctg
      541 cgacgacgcc atcggcgcgc cggtcgaacg cagcgcctgg cggcgcgcgc gcgccgtcgc
      601 cagggccgcg ctggacggct atgtgatgac cggcgtcggc acggcgacca gcttccacac
      661 ggtcagcgtc catccgggct ggtccgcgac catgcagcgc gtggccacca tcggcagcca
      721 cgtcttctac accttccgcg gccgcagcac gcaactgaat ggcgtcgtgc ggccgtccga
      781 tacgcctgcg cccgtcaccg tcgccgagcg tagcgacgac aacgccagaa ccgccctgct
      841 caacgcgctg gcccgcgacg ccggcgcgga aaaagccgcc cggccggtcg ccgcctcggc
      901 gcagatcgag ggccgcgccg cgcacgacat cgccgccgcc gtcgaaaagc ccgccaaggc
      961 gcccgcgccc gccgcctcct gatcgcgctt tcaccgatcc ccacgacgcc cgccgcgacc
     1021 cggcgggcgt tttcgttttc ccgcccttgc accgcctgcc tccgcgtccc cggcgcgcgt
     1081 tagggattcg acaaccttaa tctgacactt tgaccctcaa ccgtcttaac catgacgcgc
     1141 cgcggcgact ccgcgcgcgt cccggtcggg acggaaggca aaaaacgaat acgacctttt
     1201 gctctcagtc aggttatttt tcaggggccg ataatgctga aacccagttc caccgcgggc
     1261 ggtttgaaga tttcaaactg gtcgttcacc atcaagttca tggcgcccgc cgccatcgcc
     1321 accgccatga tcgccgtgct gaccatcggc gccgtcatgg tcatgaacgg ccagggccag
     1381 accatcgacc gcatcaacaa cgtcgtgatg ccgcaggtgc aggaactcgg cgacgtcaag
     1441 gcggcgatca aggaggacaa cgggcaactg ttccgcgccc tgacgctcaa ggccacggac
     1501 ccgacagcca acgcctccag caaggtcaac gccgtcgcca cggacctcgg caagatcgcc
     1561 gaccgcgtca agaaggacgt cgacatgacc aaggatccgg cgcagaagaa gctgctgacc
     1621 gacctcggca aggaggtcaa gacctataag gaagccgtcg aattcgccgg ttcggtgatg
     1681 gacgtcgatt tcacctcggt gccgccgctg atgtcgcagt tcgacgacgg ctacgccaag
     1741 atgtccgcgc tcagcgacca gctgatcacc gccaacgtcg ccgccgccaa gaaggccgcc
     1801 gccgacgcca aggccgccca ggccaccggc atcagccttt tgaccgcctt cgccgtcatc
     1861 atggccgtgg tgtcgggcgc catcgccttc ctgttctcgc gcgtcacggt caccggcatc
     1921 aaccgcatcg ccaagacgac cgaggaactg gccaagggca atctcgacgt cgacatctcg
     1981 accctggccc gccgcgacga actgaaaagc gtcgtggaca gcctgaacgt gttcaagtcg
     2041 aacgccgagg aaaagcagcg cctgatggcc atcgaggccg ccaccgccaa gacccgcgaa
     2101 gagcgcgcgc gtcagatgtc ggaactggcc gaacgcttcc gtcacgaggc gcaggacatg
     2161 ctggacgcgc tgtcgtccgc cgcttcggac ctcgacgcca acggccgcac gcttctgacc
     2221 atcgcgcagg agaacgagcg ccgctcgcag gacgccgtcg gctcgatccg caattcggcc
     2281 gacaatgtgc agaacgtcgc ctcggcgacc accgagctgt ccgcctcgat cggcgtgatc
     2341 ggcgaccagg ccgtgcgttc ggtggaaatc gccgccgaag ccgtgtcgga agccgaccgc
     2401 accaacgaat cgatggccga gctgagccgc gccgccgacc agatcggcga ggtcgtcgac
     2461 ctgatcaacg ccatcgcgca gcagaccaac cttctggccc tcaacgccac catcgagtcg
     2521 gcgcgcgccg gcgaggcggg caagggcttc gcggtcgtgg cctcggaagt gaagtcgctg
     2581 gcgcaacaga ccgccaaggc gaccgacgaa atccgcgacc gcatcaagga catccaggcc
     2641 gccgcgcaaa acggcgtcgg cgccatccgc ggcatcggcg agacgatcaa gcacatgaac
     2701 gagatcgccg cctcgatcgc cgactcggtg catcagcagg gcgacgccac caacgaaatc
     2761 gcccgcaacg tcaacgaggc ctccgacggc acttcgatgg cttcgagctc ggtggcgcaa
     2821 ctgtccgcct cggccgccga caccgaaaag gcgtcgaccg aaatgctggg cgccgccaac
     2881 cagctgaccc agcgcaccca ggcgatgagc gagaatatcc gccgcttcct gagcgaactg
     2941 accgctgcct aagcccag
//
LOCUS       DASVLL010000234         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_498508,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000234 DASVLL010000000
VERSION     DASVLL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498508"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VF402_03855"
     CDS             <1..363
                     /locus_tag="VF402_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator protein"
                     /protein_id="HEX7799458.1"
                     /translation="PGYDLKLTEAAASREPLLHMTRAGGGGQPPAGDVVQAKPAAAPV
                     THTPGRNDSEPRNYLTDETLAHLRRHYRGWDFHALHAEFRAWVREAADREPDRYQSAF
                     IGFVKRYHDKHRHQLRAG"
     gene            complement(847..2451)
                     /locus_tag="VF402_03860"
     CDS             complement(847..2451)
                     /locus_tag="VF402_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HEX7799459.1"
                     /translation="MTPKIGRRGVLAGMAAVAALPSAAAAQAPSTGAPAALPDPAQAD
                     APVYRTDNARWQAAYDKALGVLAGNVQVLPRFSAPVLIEGADYAGVWMECGPHEALVY
                     RRFRPDVARNTHMTFFALQRMDGQLPYNNKATGTGFGQIQMVVPIAATAWELARATGD
                     QELLETAYRAGAAWDGWLTRWRNTRGTGLVEGFCTYDTGMDNSPRWAGIPPQCPNQDA
                     KRFPAGLALPRLCPDLSATSFGARTALSQMAAALGRPDAAAHWAEQADAIRARILSVL
                     YVAGDAAFYDLDANGAFVKTRSDILSRVCGEHVVDQALFDDLWTRQIHNPSAFWARYP
                     LPSVALDDPAFVRPIAANSWGGPSQALTALRAGRWLDHYGRGSAFAQMMERWCEAIMA
                     DMTFRQQIDPLSGQFSDGLPGYSPCALVMIDYTWRLAGVVETPDALHWNVRPGHAAAQ
                     GARFAMKTDAGVTAQMVYDGKGADLTLGGKALGRVEGAARLVTDKAGRPLSLRGVSEQ
                     AQAVVLRRPGHPVLRARLLPDQDLTF"
     gene            complement(2448..>2690)
                     /locus_tag="VF402_03865"
     CDS             complement(2448..>2690)
                     /locus_tag="VF402_03865"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HEX7799460.1"
                     /translation="GPAAGRIVQLRAEVSPDQSVRFSYSLDDGRSFAPLGEAVPLSRF
                     SWWKGSRPALFSYTTGKAKAGYADFDWLHVETEDRP"
BASE COUNT          467 a          926 c          874 g          423 t
ORIGIN      
        1 cccggctacg acctgaaact gaccgaagcc gccgcctcgc gcgagcccct cctccacatg
       61 acccgcgcgg gcggcggcgg gcaaccgccc gcgggggatg ttgttcaggc taaacccgcc
      121 gccgcgccgg tgacgcatac gccgggtcgc aacgattccg aaccacgcaa ctatctcacc
      181 gacgagacgc tcgcccatct gcgccggcat tatcgcggct gggacttcca tgccctgcac
      241 gccgagttcc gcgcctgggt ccgcgaagcg gccgaccgcg agcccgaccg ctaccaaagc
      301 gccttcatcg gcttcgtaaa gcgctatcac gacaagcacc gccatcagct ccgcgccggt
      361 taaggcgatt ttcgccacga aaagcacgaa aaggcacgaa atgaaaaaag cctcaaagca
      421 aagctttgag gatcgaaaag gcgcaagcaa gatatagttt tcgtgttttt tcgtgctttt
      481 cgtggcgaaa aaactacctc tctcgcacgc cgttcccgaa gtgacgaatc acccgccgct
      541 atcctctcgt cccgccgggg ataagcttcg gcatccgcat cgttttgaac gagtttcgcg
      601 cgccgattcg cgaaagagat tcaaatctat ccccgctgcc tgcgggtttc gacacatcgg
      661 gaacgggttt cgttctttcg ggaactatcc cctgacacat cgggaacgaa aacccgacac
      721 atcgggaacg acgtatcggg ctaaggcctt gataaaactg gtttaaaagc gcaaatgcag
      781 gcgtctaact tttctaaaag actctataac ccctctaacc ctctctcagg gatttttaaa
      841 gatatttcag aaggttaagt cttgatccgg cagcaaccgc gcccgcagga ccggatggcc
      901 gggacgccgc aacacgaccg cctgcgcctg ttcggaaacg ccgcgcaggg acagcggcct
      961 gcccgccttg tcggtgacca gccgcgccgc gccctcgacg cggcccagcg ccttgccgcc
     1021 caaggtgagg tcggcgccct tgccgtcata gaccatctgc gccgtcacgc ccgcatccgt
     1081 cttcatcgca aaacgcgcgc cctgcgccgc cgcgtggccg ggccggacgt tccagtgcag
     1141 cgcgtccggc gtctcgacca ccccggccag ccgccaggtg tagtcgatca tgaccagcgc
     1201 gcagggcgaa tagccgggca ggccgtcgct gaattggccg ctcagcggat cgatctgctg
     1261 gcggaaggtc atgtcggcca tgatcgcttc gcaccagcgc tccatcatct gcgcgaaggc
     1321 gctgccgcgg ccgtaatggt cgagccagcg cccggcgcgc agggcggtca aggcctgcga
     1381 cggcccgccc cagctattgg ccgcgatcgg gcgcacgaag gccggatcgt cgagcgcgac
     1441 cgagggcagg ggatagcgcg cccagaaggc ggacggattg tgaatctgcc gcgtccacag
     1501 atcgtcgaac agggcctgat cgacgacatg ctcgccgcac acgcgcgaca ggatgtcgct
     1561 gcgcgtcttg acgaaggcgc cgttcgcgtc gaggtcgtag aaggcggcgt ccccggcgac
     1621 atagaggacg ctcaggatcc tggcgcggat cgcgtcggcc tgttcggccc agtgcgcggc
     1681 ggcgtccggc cggcccagcg ccgccgccat ctgcgacagg gccgtgcgcg cgccgaagct
     1741 cgtcgccgac aggtccgggc acaggcgggg cagggcgagc ccggcgggaa agcgtttggc
     1801 gtcctgattg gggcattgcg gcggaatccc ggcccagcgc gggctgttgt ccatgccggt
     1861 gtcgtaggtg cagaagccct cgaccagccc cgtgccgcgc gtgttgcgcc agcgcgtcag
     1921 ccatccgtcc cacgccgcgc cggcgcgata ggccgtctcc agcagctcct gatcgccggt
     1981 ggcgcgcgcc agctcccagg cggtggcggc gatggggacg accatctgga tctggccgaa
     2041 gccggtcccg gtcgccttat tgttgtaggg caactgcccg tccatgcgtt gcagggcgaa
     2101 gaaggtcatg tgcgtgttgc gcgccacgtc ggggcggaag cggcgataga cgagggcttc
     2161 gtgcggcccg cactccatcc acacgcccgc atagtcggcg ccttcgatca gcaccggcgc
     2221 ggaaaagcgc ggcagcacct gcacattgcc cgccagcacg cccagcgcct tgtcgtaggc
     2281 ggcctgccag cgggcgttat cggtgcgata gacgggcgcg tcggcctgcg cagggtccgg
     2341 caaggcggct ggagcgcccg tggagggcgc ttgcgcggcg gcagcgctgg ggagggccgc
     2401 gacggcggcc atgcccgcca gaacgcctct gcggccgatt ttgggcgtca tggccgatcc
     2461 tccgtctcga catgcagcca gtcgaagtcg gcgtaaccgg ccttcgcctt gcccgtcgtg
     2521 tagctgaaca gggccgggcg gctgcccttc caccacgaaa agcgcgacag cggcacggcc
     2581 tcgcccagcg gcgcgaagct ccggccatcg tcgaggctgt agctgaagcg cacgctctgg
     2641 tccgggctga cctcggcgcg cagttgcacg atgcggcccg ccgccggtcc
//
LOCUS       DASVLL010000235         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_336109,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000235 DASVLL010000000
VERSION     DASVLL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336109"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="VF402_03870"
     CDS             complement(<1..1397)
                     /locus_tag="VF402_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799461.1"
                     /translation="MSRILSNGGLRGRLLTCAGLAALSACLPLSATAQTADAADPAPA
                     NAKAKAADTTTVVVTGIRKGIQDAVTAKKKDSSIIEAVSAEDIGKLPDASIAESIGRL
                     PGIAAQRDKGRAQTLSIRGLGPDFTVTTLNGREQASTNNNRSVEYDQYPSEIISQVKV
                     YKTPDAGMSYQGIAGTVDLETVRPLSYGKRALSTSYQYEDNAYKAVVPGMPTHGDRYN
                     ITYIDQFADKTIGLALGVAHTKAPYQARTHEPWGYPACGQCSAADANDLVIGGDKSGM
                     QSSYYDRTSYFGTLEFKPNDRFHAVFDAFHSDFTELQTVRRMEYGTIWGTGVLQPGYT
                     VADGRIASGEFDGVTTIVENYNYLRHAKIDNFGLNLNYDLNDKWTLNADISDSKVHRT
                     DLRLESTGGTGPAGSVVKNDVTFTTDADGVTHFHSDFDFSDFDKMFLTDPGGWGGPDT
                     RAGYVGNPTVNDEIKA"
     gene            1889..2905
                     /locus_tag="VF402_03875"
     CDS             1889..2905
                     /locus_tag="VF402_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7799462.1"
                     /translation="MNRRPTSLDIAAQAGVSQSTVSRVINRSALVSDDVRRRVLDAAQ
                     QLHYKIDANARQLRSRRINTIALLVLEDMEDERSNINPFFLPMIGAIAQYAADKGFDL
                     VFSLQREDNGWGAGYCLSRPAEGIIFLGSKNFETYAENFRGYNHSNDCWVVWGLDKAP
                     DGTVCIASDNEGGAFSAVNHLLRSGRRRIAFIGNLHSPHWEFVERFAGYRRALRAAGI
                     EIDSGLCVDSCLTLEDGAEAAARLMASGTAFDAVFAQTDMLAVGAMRYLLSRGVRIPQ
                     QVAVMGFDDLWIGRAVAPALSTVRQDTQNAGRLLVERVAALIDGQKIDGARIPTELAL
                     RDSA"
     gene            3031..>3337
                     /locus_tag="VF402_03880"
     CDS             3031..>3337
                     /locus_tag="VF402_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799463.1"
                     /translation="MTSSIAFRRLLLTTSFLALAAPVLAHADDAPGKPDKAPAEITVI
                     GKRVIKGSAGATGLDLSLRQTPQSVTVVDEQQIKDFSLTTANQVLTSIPGIQVDAAET
                     "
BASE COUNT          561 a         1060 c         1058 g          658 t
ORIGIN      
        1 gccttgatct cgtcgttcac ggtcggattg ccgacatagc ccgcgcgcgt gtccgggccg
       61 ccccacccgc cgggatcggt caggaacatc ttgtcgaagt cgctgaagtc gaaatcggaa
      121 tggaaatgcg tgacgccgtc cgcgtcggtc gtgaaggtga cgtcgttctt gaccaccgac
      181 cccgccggtc ccgtgccgcc ggtcgattcc agccgcaggt cggtgcggtg gaccttcgag
      241 tcgctgatgt cggcgttcag cgtccacttg tcgttcagat cgtaattcag gttgaggccg
      301 aaattgtcga tcttggcgtg gcgcagatag ttgtagttct cgacaatggt ggtgacgccg
      361 tcgaattcgc ccgaggcgat gcgtccgtcc gcgaccgtat agcccggttg cagcacgccc
      421 gtgccccaga tcgtgccgta ctccatgcgc cgcacggtct gcaattcggt gaagtcggaa
      481 tggaaggcgt cgaacaccgc gtggaagcgg tcgttcggct tgaactccag cgtgccgaaa
      541 taggaggtgc ggtcgtaata ggacgactgc atcccgctct tgtcgccccc gatgaccaga
      601 tcgttggcgt cggcggcgga acattgaccg caggccggat agccccacgg ttcgtgcgtg
      661 cgcgcctgat agggcgcctt ggtgtgcgcg acgcccagcg ccagaccgat cgtcttgtcg
      721 gcgaactggt cgatataggt gatgttgtag cggtcgccgt gcgtcggcat gcccggcacg
      781 acggccttgt aggcgttgtc ctcgtactgg tagctcgtgg acagggcgcg cttgccgtag
      841 gacagcgggc gcaccgtctc cagatcgacc gtcccggcga tgccctgata ggacatgccc
      901 gcgtccgggg tcttatagac cttgacctgg ctgatgattt ccgacgggta ctggtcgtat
      961 tcgaccgagc ggttgttgtt ggtgctggcc tgttcgcggc cgttcagcgt ggtgacggtg
     1021 aagtccggcc caagaccccg gatcgacagg gtctgggcgc ggcccttgtc gcgctgcgcc
     1081 gcgatgccgg gcaggcgtcc gatggattcg gcgatcgagg cgtccggcag cttgccgatg
     1141 tcctcggcgg acaccgcctc gatgatcgac gagtccttct tcttggccgt gacggcgtcc
     1201 tgaatcccct tgcggatgcc cgtgaccacg accgtggtcg tgtccgcggc cttggctttg
     1261 gcgttcgcgg gcgccgggtc ggcggcgtcc gccgtttgcg ccgtagcgct caacggcagg
     1321 caggcgctca gggcggccag ccccgcgcag gtcagcaggc ggccgcgcag gccgccgttc
     1381 gataagatac gcgacatagt ttctctccct tcctgaccga tgcgccgggc ttgcgcgcgg
     1441 cggcatccgt cccggcgttt aagccggata tgtaggtctc aaccctctgg ccgttatggt
     1501 tttgtttcgg ctctgtcgcg acctgataca gatcgccggg gaagggagca atattggtaa
     1561 cgttatcgta tgttataaat aaacataagt tgcaatattg caacttaatt tacatatttt
     1621 tatagtaata cgtattcata aatattgcaa tatattatat tgaatcgaga aatcagacgc
     1681 atttacctga ccctaaattc acaatcatgc cgcatatcgc gtccgataaa aaccctttat
     1741 aagggtgtgg gtacgtattc atggcgtttc gcgccgcgaa cctttgaacc ggggcgcggg
     1801 aatacgtatt cttgttgccg ggcgcgcggc gcgcttctta actgcgcccg cgaaagcgca
     1861 accggacggg ccggatacgg ggatccagat gaacagacga ccgacatcgc tcgacatcgc
     1921 cgcccaggcg ggcgtttcgc agtccaccgt ttcgcgcgtg atcaaccgct cggccctggt
     1981 cagcgacgac gtgcgccgcc gcgtgctcga cgccgcccag cagctacact acaagatcga
     2041 cgccaacgcc cgtcagttgc gctcgcgccg catcaacacc atcgccctgc tggtgctgga
     2101 ggacatggag gacgagcgca gcaacatcaa tcccttcttc ctgccgatga tcggcgcgat
     2161 cgcgcaatac gcggcggaca agggcttcga tctcgtcttc tccttgcagc gcgaggacaa
     2221 tggctggggc gcgggctatt gcctgtcgcg gcccgccgag ggcatcatct tcctcggctc
     2281 gaagaatttc gagacctatg ccgagaattt tcgcggctat aatcacagca acgactgctg
     2341 ggtggtgtgg ggactggaca aggcgccgga cggcacggtc tgcatcgcca gcgacaatga
     2401 gggcggcgcc ttttcggcgg tcaaccacct gctgcgctcc gggcggcggc gcatcgcctt
     2461 tatcggcaac ctgcacagcc cgcactggga gttcgtcgag cgcttcgcgg gctatcgccg
     2521 cgccctgcgc gccgccggga tcgagatcga ctccggcctg tgcgtcgaca gttgcctgac
     2581 cttggaggac ggcgccgagg ccgccgcgcg gctgatggcg tcggggaccg cgttcgacgc
     2641 cgtcttcgcc cagaccgaca tgctggccgt cggcgccatg cgctatctcc tgagccgggg
     2701 cgtgcgcatc ccgcagcagg tcgccgtcat gggtttcgac gacctgtgga tcggccgcgc
     2761 cgtcgccccc gccctgtcca cggtgcgtca ggacacccag aacgccgggc gcctgctggt
     2821 cgagcgggtc gccgccctta tcgacggcca gaagatcgac ggcgcgcgga ttccgaccga
     2881 actggcgttg cgggattccg cctgaggcgc aaagccgggc tttgcgccat gtgtcgcggc
     2941 tgaaatgaga ataattcgca aatgctgttg acgtggcgcg tctgagcgcg taaccacgcc
     3001 gctattgcaa atcattctca caagtgcgcc atgacctcct ccatcgcctt ccgccgccta
     3061 ctcctgacga cctcgttcct ggccctcgcc gcgcccgttc tggcgcacgc cgatgacgct
     3121 ccgggcaagc cggataaggc gcccgcggaa atcaccgtca tcggcaagcg cgtgatcaag
     3181 ggctcggcgg gcgccaccgg cctcgacctc agcctgcgcc agacgccgca atcggtcacc
     3241 gtggtggacg agcagcagat caaggacttt tcgctgacca cggccaatca ggtgctgacc
     3301 tccataccgg gcatccaggt cgacgccgcc gaaaccg
//
LOCUS       DASVLL010000236         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_498553,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000236 DASVLL010000000
VERSION     DASVLL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498553"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..463
                     /locus_tag="VF402_03885"
     CDS             164..463
                     /locus_tag="VF402_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799464.1"
                     /translation="MTVLRVLLAAVWLLFGFAAGAHAQPSAPPCHMAMTTHHAPAPSG
                     DPGVMPCCSQPALIAPDAVLVPQAHRVAPLRLSPPPVRAMAGLSTPAEPRPPKNA"
     gene            508..1674
                     /locus_tag="VF402_03890"
     CDS             508..1674
                     /locus_tag="VF402_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799465.1"
                     /translation="MSYRCLAPAFTAALLPLSAHAQMSGMDMTPKSGWTVMAHTTLTL
                     AADDQQGPRGGSKTFLEGMSMIMADRALSADTDLELQAMLSPDPFMGKSGYPLLLQTG
                     ETADGATPLIDRQHPHDLFMGLTASVRHRFGDGVSGFVRAGWPGENGFGPQTFMHRPS
                     GEMFPTAPITHHWFDSGHITMGVVTAGLSKGPLQLEVSGFTGREPDQHRFNLDRPRLD
                     SASARVSWRITNDLSAQASWAHVVSPEQIEPDVNLIQKSVSLAYGHSFSAGTLDSTLA
                     FGRKSVTGGDEKPSDALLFENTFHFNGPWLGLLRHERVYNDELVPGAAYWVGKTEIGV
                     ARDIALPRGIKLSVGVVRQFNAIPGALRGVYGGHPDGTVAFLRLTQHWMPGMEM"
     gene            1732..2544
                     /gene="hutG"
                     /locus_tag="VF402_03895"
     CDS             1732..2544
                     /gene="hutG"
                     /locus_tag="VF402_03895"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEX7799466.1"
                     /translation="MPNLPPTDETPPYEFQAGDGPLVVAAPHVGTHLPPGVAAAMNPV
                     GLSVGETDFHVHRLFDFAPEMGASTLFATHSRYVVDLNRPPDDARLYPGRFETGLCPL
                     SDFDRNPIYPPGREPDAAEIAARRERYWRPYHDRLRAALDAAKARHGFALLIDAHSIR
                     PRIPSLFDGALPDLNFGTDSGRTLPPRLTGALNGWRAAQARWSHVLDGRFTGGYTTRH
                     YGQPRDGIFALQIEIVQDAYLDLKAPRLYDAAIARPLSLALRALTETLLAAL"
     gene            2557..>3067
                     /locus_tag="VF402_03900"
     CDS             2557..>3067
                     /locus_tag="VF402_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX7799467.1"
                     /translation="MTTSRGNIIDGAGYAAALRERLKSHVADLKAAHGLTPCLVVVIV
                     GEDPASQVYVKSKGEQTLAIGMESRTIRLPDTTSEAELLSLIAELNAERAVNGILVQL
                     PLPPHMSSLKVIDAIHPDKDVDGFHIANAGRLAVGLPGIVPCTPLGSLMLLQDAMRTH
                     EDSFAGKHAV"
BASE COUNT          490 a         1134 c          961 g          482 t
ORIGIN      
        1 gcctgacgca aggccgcgac gcgcaggagg accagatccg gcgcaacatc gggcaggtca
       61 tggcgttcga aaaattcaag gccaggctgc cgaaaaagcc cgaagacctg ctgctcgacc
      121 tcggcaaggc gctggggcgg ctgagattcg cccgcgacgc cgcatgaccg tgttgcgcgt
      181 cctgctggcg gcggtctggc tgctgttcgg tttcgccgcg ggcgcgcacg cccagccttc
      241 ggcgccgccc tgccatatgg cgatgaccac gcaccacgcg cccgcgccgt ccggcgatcc
      301 gggcgtcatg ccctgttgca gccagcccgc cctgatcgcg ccggacgccg tcctcgtccc
      361 gcaggcgcac cgcgttgcgc cgctgcgcct gtcgcctccg cccgtccgcg ccatggccgg
      421 actgtccacg cccgccgagc cgcgcccgcc gaaaaacgcc tgatggtctg agcttcatcc
      481 catcgaccgt ttttcggaga cttttccatg tcttatcgtt gcctcgcgcc cgccttcacg
      541 gcggcgcttc tgcccctgtc cgcccacgcc caaatgtccg gcatggacat gacgcccaaa
      601 tccggctgga ccgtcatggc gcacaccacc ctcaccctcg ccgccgacga ccagcagggg
      661 ccgcgcggcg gctccaagac cttcctcgaa ggcatgagca tgatcatggc cgaccgcgcc
      721 ctgagcgccg acaccgatct ggagcttcag gccatgctgt cgcccgatcc gttcatgggc
      781 aagagcggct atccgctgct gctgcaaacc ggcgaaacgg cggacggcgc gacgccgctc
      841 atcgaccgcc agcacccgca cgacctgttc atgggcctga ccgcaagcgt gcgccaccgc
      901 ttcggcgacg gcgtcagcgg cttcgtgcgc gccggatggc cgggcgaaaa cggcttcggg
      961 ccgcagacct tcatgcaccg gccttcgggc gagatgttcc cgaccgcgcc gatcacgcac
     1021 cactggttcg attccggcca catcaccatg ggcgtggtca cggcggggct gagcaaaggc
     1081 ccgctccagc tcgaggtcag cggcttcacc ggacgcgagc cggaccagca tcgcttcaac
     1141 ctcgaccgcc cgcgcctcga ttccgcctcg gcgcgcgtaa gctggcggat cacgaacgac
     1201 ctgtcggcgc aggcgtcctg ggcgcacgtc gtcagccccg aacagatcga accggacgtc
     1261 aacctgatcc agaaaagcgt cagcctcgcc tatggccaca gcttttccgc cgggacgctc
     1321 gattcgaccc tggccttcgg gcgcaagagc gtcaccggcg gggacgaaaa gccgtccgac
     1381 gcccttttgt tcgagaacac gtttcacttc aacggtccgt ggctcggcct tttgcgccat
     1441 gagcgcgttt ataatgacga gcttgtcccc ggcgccgcct actgggtggg caagaccgag
     1501 atcggcgtcg cgcgcgacat cgcccttccg cgcggcatca agctgagcgt cggcgtcgtg
     1561 cgccagttca acgccatccc cggcgcgctg cgcggcgttt acggcggcca tcccgacggg
     1621 acggtcgcct tcctgcgcct gacgcagcac tggatgcccg gcatggagat gtagaggcgc
     1681 ggaaccggcg tccggcttga cagacgcgcg gcggaaggct caactgaaac catgccgaac
     1741 cttccgccca cggacgaaac gccgccgtat gagtttcagg cgggcgacgg cccgctggtc
     1801 gtggcggcgc cgcatgtcgg cacgcatctg ccgccgggcg tcgccgccgc gatgaacccg
     1861 gtggggttga gcgtcggcga aaccgatttt cacgtccacc gcctgttcga cttcgcgccg
     1921 gagatgggcg cttcgacgct gttcgccacc cattcgcgct atgtcgtcga tctcaaccgc
     1981 ccgcccgacg acgcgcgcct gtatccgggc cggttcgaga cggggctttg cccgctcagc
     2041 gatttcgacc ggaacccgat ctatccgccg ggccgcgaac ccgacgccgc cgaaatcgcc
     2101 gcgcggcgcg aacgttattg gcgcccttat cacgaccgcc tgcgcgccgc gctcgacgcg
     2161 gcgaaggcgc ggcatggctt cgcgctgctg atcgacgccc attcgatccg cccgcgcatc
     2221 cccagcctgt tcgacggcgc cctgcccgac ctcaatttcg ggacggattc aggccgcacc
     2281 ctgccgccgc gcctgaccgg agcgctgaac ggctggcgcg cggcgcaggc gcgctggagt
     2341 cacgtcctcg acgggcgctt caccggcggc tacaccacgc gccattacgg ccagccgcgg
     2401 gacgggattt tcgcgctgca aatcgagatc gtgcaggacg cctatctgga cttgaaagcg
     2461 ccgcgcctgt atgacgccgc catcgcccgt cctttaagtc tggccctgcg cgcgctgacc
     2521 gaaacgctgc tcgcggccct ttgagagagc ctccccatga cgacatcccg cggcaatatc
     2581 atcgacggcg ccggttacgc cgccgccttg cgcgaacggc tgaaaagcca tgtcgccgat
     2641 ctgaaggccg cgcacggcct gacgccctgc ctcgtggtgg tgatcgtcgg cgaggatccg
     2701 gccagtcagg tctatgtcaa gagcaagggc gagcagaccc tggccatcgg catggaatcg
     2761 cgcaccatcc gcctgccgga cacgacctcc gaagccgaac tgctgagcct gatcgcggag
     2821 ctgaacgccg agcgcgcggt caacggcatt ctcgtgcaac tgccgctgcc cccacacatg
     2881 tcgtcgctca aggtgatcga cgcgattcac ccggacaagg acgtcgacgg cttccacatc
     2941 gccaacgccg gacgtctggc cgtgggcctg cccggcatcg tcccctgcac gccgctgggt
     3001 tcgctgatgc tgctgcaaga cgcgatgcgg acccatgaag acagcttcgc gggcaagcac
     3061 gcggtca
//
LOCUS       DASVLL010000237         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_175305,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000237 DASVLL010000000
VERSION     DASVLL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175305"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /gene="trmD"
                     /locus_tag="VF402_03905"
     CDS             complement(<1..649)
                     /gene="trmD"
                     /locus_tag="VF402_03905"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7799468.1"
                     /translation="MSDAPPPYQVTVMTMFPEAFPGPLGVSLIGTAWKESARWALETL
                     DIRDFSSDKRGFLDDTPAGGGPGQVLKADVIAKALDSLEVSGKPLVYMSARGRKLTQE
                     KVREWAKADGLIVLCGRFEGVDQRVLDARGFEEISVGDAVLAGGEAAAMVTIEAVVRL
                     LPGVLGAAASLDRESFEDGLLEEPQYTRPRRFEGLDIPDVLLSGDHKKVAQWKQQQ"
     gene            complement(689..>2695)
                     /locus_tag="VF402_03910"
     CDS             complement(689..>2695)
                     /locus_tag="VF402_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799469.1"
                     /translation="VGVVIDGVSRARTATAMSDLGPLDRIEILKGPQSDVYGKGASAG
                     VIQVVSKLPSFTPDTEVTLDAGNMGAYGASLYTTGKLSDHWAGSLFVTKRKRDGQYHV
                     HTGDGPATRRDDGDQNYWSARGQLLYVPNDRASLRLIADYTDRDEACCAAVNIAIGGT
                     AGYIDALANGDGTAASVDPKARQVWSNRPLNQRLKDGGVSAQLDLKLNDAIQLTSITA
                     VRRWDHANGYDGDFTAADVLYRDPGGLRNRFDTFSQEVRLNGTGERYNWMAGLYLDQS
                     ELDRRDETLYGADYESYVSLLLSGGASLSKVADYTGLPVGDSFVAGQGSHDAYDQREK
                     NFAVFGHGEWKLSDSLTLLGGLRWNHQIKRVVSRFSNSDGGLACALATGGQGVLCLPW
                     SNPAFNGLTLYQRNAEDATTGSLRLKWQATTNLMAYASYATGWKGTGFNLDREQNADM
                     SVDRDSSFKPETSRSYEAGVKGRWFGNRLSVNLAAFDEAFRNFQLNTYLGTTFLVYSV
                     PHLRSRGVELDGRYYLGGLSLNAGLTYDDAKFGPEAVPGLPLIANGTASFAPKWSAAY
                     GGDYSRDFGLYRLGVSLSAKYNSAYNTGSDLAPVKTQKAYTLLTGRVALSRPDQGWSL
                     ELWGQNLSDATYYQVAFAAPFQSGTYDAFLGQPRTYGVTLKLSY"
BASE COUNT          441 a          892 c          895 g          467 t
ORIGIN      
        1 tttgctgctg cttccattgc gcgacctttt tgtggtcgcc ggacaacaac acatcgggga
       61 tgtcgaggcc ctcgaagcgc cgcggtcgcg tgtactgcgg ctcctcgagg agaccatcct
      121 cgaaactctc gcgatccaga ctggccgccg cccccagaac gccggggagc agacgcacga
      181 ccgcctcgat cgtcaccatc gccgccgctt cgccgcccgc aaggaccgcg tcgccgacgc
      241 tgatttcctc gaacccgcgc gcgtccagca cgcgctgatc gaccccttcg aagcgcccgc
      301 acaagacgat cagcccatcg gccttcgccc attcgcgaac cttctcttgc gtcagcttcc
      361 ggccgcgcgc gctcatatag acgagcggct ttccagaaac ctcaagcgaa tcaagcgctt
      421 tggcgatcac gtccgccttg agcacctgac ccggcccgcc gccggcgggg gtgtcgtcga
      481 gaaagccgcg cttatcgctc gaaaagtcgc gaatgtccag cgtttccaag gcccagcgcg
      541 cagattcctt ccacgccgtc ccgatcagcg acacgccgag cggccccgga aacgcttccg
      601 ggaacatggt catcaccgtg acctgatagg gtgggggcgc gtcggacatg gccgcgcccc
      661 ttagcacacc tgcccctaaa ggcaaaatct aatagctcag tttcagcgtc acgccatagg
      721 tgcgcggctg gcccaggaag gcgtcgtagg ttccgctctg gaacggcgcg gcgaaggcga
      781 cctgataata ggtcgcgtcg ctgaggttct ggccccacag ctccagcgac cagccctgat
      841 ccgggcggct gagcgcgacg cggccggtga gcagggtata ggccttctgc gtcttgaccg
      901 gcgcgaggtc cgagccggtg ttgtaggcgc tgttgtactt ggcgctcagg ctgacgccga
      961 gccggtagag gccgaaatcg cggctgtagt cgccgccata ggccgccgac catttcggcg
     1021 cgaacgaggc ggtcccgttc gcgatcagcg gcaggcccgg cacggcttcg gggccgaatt
     1081 tggcgtcgtc ataggtcagg cccgcattga ggctgaggcc gccgagataa tagcgcccgt
     1141 ccagctccac gcccctggag cgcagatgcg gcacggaata gacgaggaag gtcgtgccca
     1201 gataggtgtt gagctggaag ttgcggaagg cttcgtcgaa cgcggccaga ttgacgctca
     1261 gccggttgcc gaaccagcgg cccttgacgc ccgcctcata ggatcgcgag gtttccggct
     1321 tgaacgacga gtcgcggtcg acgctcatat cggcgttctg ctcgcggtcg agattgaagc
     1381 ccgtgccctt ccagccggtg gcgtaggacg cataggccat caggttcgtc gtcgcctgcc
     1441 atttgaggcg caaggagccg gtggtggcgt cctcggcgtt gcgctgatag agcgtcaggc
     1501 cgttgaaggc cgggttcgac cacggcaggc acagcacgcc ctgaccgccg gtggccagcg
     1561 cgcaggcgag gccgccgtcg gaattggaga agcggctgac gacgcgcttg atctgatggt
     1621 tccagcgcag gccgcccagc agggtcaggc tgtcgctgag cttccattcg ccgtggccga
     1681 agacggcgaa gttcttttcg cgctggtcat aggcgtcgtg gctgccctga cccgcgacga
     1741 aggagtcgcc gaccggcagg ccggtatagt ccgcgacctt cgacaggctg gcgccgcccg
     1801 acagaagcag gctgacatag gattcgtaat cggcgccgta cagcgtctcg tcgcggcgat
     1861 ccagttcgct ctggtcgaga taaagcccgg ccatccagtt gtagcgctcg cccgtcccgt
     1921 tcaggcgcac ctcttgggaa aaggtgtcga agcggtttct gagcccgccc ggatcgcggt
     1981 acagcacgtc ggcggcggtg aaatcgccgt catagccgtt ggcgtggtcc cagcggcgca
     2041 cggcggtgat cgaggtcagt tgaatcgcgt cgttcagttt caggtcaagc tgcgccgaca
     2101 cgccgccgtc cttcagccgc tggttcaacg ggcggttgga ccagacctga cgcgccttcg
     2161 gatcgacgct ggcggcggtc ccgtcgccgt tggccagggc gtcgatatag cccgccgtgc
     2221 cgccgatggc gatattgacc gccgcgcagc acgcttcgtc gcggtcggtg tagtcggcga
     2281 tcaggcgcaa ggaggcgcgg tcgttcggca catagagaag ctgcccgcgc gccgaccaat
     2341 agttctggtc gccgtcatcg cggcgcgtcg ccgggccgtc gccggtgtgg acgtgatatt
     2401 ggccgtcgcg cttgcgcttg gtcacgaaca gcgatccggc ccagtggtcg ctcagcttgc
     2461 cggtggtgta gagcgaggcg ccgtaagcgc ccatattgcc ggcgtcgagc gtgacctcgg
     2521 tgtccggcgt gaacgacggc agcttcgaca ccacctgaat cacgcccgcc gaggcgccct
     2581 tgccgtacac gtccgactgc ggccctttca ggatttcgat gcggtccagc gggccgaggt
     2641 cgctcatcgc cgtggcggtg cgggcgcgcg acacgccgtc gatcaccacg ccgac
//
LOCUS       DASVLL010000238         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_175466,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000238 DASVLL010000000
VERSION     DASVLL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175466"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..2158
                     /gene="ftsH"
                     /locus_tag="VF402_03915"
     CDS             245..2158
                     /gene="ftsH"
                     /locus_tag="VF402_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7799470.1"
                     /translation="MNLRSMAIIGVIAALVIGVVSFMHMKSGPLMTAEGGTTELAYSD
                     LLNAVQAGQVKDITIHNDEARGALSGNKTFHAVIPLYDHKTLDDALVAKGVKTNYQTT
                     RASILMSVLTTIVPVLLMVGLWFIIMRQMQGGGRGGAMGFGKSKARLLTEHKNRVTFA
                     DVAGVEEAKEELQEVVDFLKDPSKFQKLGGKIPKGALLVGPPGTGKTLLAHAVAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGHGGGN
                     DEREQTLNQLLVEMDGFEAQEGIIIIAATNRPDVLDSALLRPGRFDRQVTVPNPDLTG
                     REAILRVHMKQVPLAVDVDVKVIARGTPGFSGADLANLVNEAALMAARKDRKLVTMRD
                     FEDAKDKVMMGAERRSMAMSEDEKKLTAYHEGGHAIVALKAPQADPVHKATIIPRGRA
                     LGMVMQLPEGDRYSQNKIQMESRLAILMGGRVAEELIFGKDAVTSGASSDIQQATKLA
                     KAMVTRWGYSDELGLVNYRDSEDEYGAYARDVSEETSRKIDAEIKALVQKGYDEAKRI
                     LTEYNDGLHRLARTLLELETLTGDEIAKVLNGEAIERDEDTGVAIELSSNSAVPETET
                     PKAEPSIVPVVAE"
BASE COUNT          409 a          680 c          750 g          331 t
ORIGIN      
        1 tgcgcggcgc gctgccggtg gtggacggcg ccctggcgcc ggcggcgtgc tgggtgacgt
       61 cgcgcttcaa ggcggcggcg ggcgccttcg ccaccgagcg cgatccggcc gccgtcccga
      121 aaagaaccgg cgcgtgaaaa cctctgtggc gaagcgcgca tttcctccct atatgtgagg
      181 acaagatgaa aaagcccccc ggaggggcgg cctcgatcgg gccagtttaa ggattgaata
      241 aggcatgaac ctgcggtcca tggcgattat cggcgttatc gcggcgctcg tgatcggcgt
      301 cgtttccttt atgcacatga agtcggggcc gctgatgacg gcggaaggcg gcacgaccga
      361 gctggcctat tccgacctgc tgaacgcggt gcaggccggg caggtcaagg acatcaccat
      421 ccataacgac gaagcgcgcg gcgcgctgag cggcaacaag accttccacg ccgtcattcc
      481 gctgtacgat cacaagacgc tggacgacgc gctggtcgcc aagggcgtca agaccaatta
      541 ccagacgacg cgcgcctcga tcctgatgag cgtcctgacc accatcgtgc cggtgctgct
      601 gatggtcggc ctgtggttca tcatcatgcg ccagatgcag ggcggcggac gcggcggcgc
      661 gatgggcttc ggcaagtcca aggcgcggct tctgaccgag cacaagaacc gcgtcacctt
      721 cgccgacgtc gcgggcgtcg aggaggccaa ggaggagttg caggaggtcg tcgatttcct
      781 gaaagatccg tccaagttcc agaaactggg gggcaagatt ccgaagggcg ccctgctggt
      841 cggtcctccg ggcacgggta agaccctgct ggcgcacgct gtcgccggcg aggcgggcgt
      901 gcccttcttc tcgatctccg gctccgactt cgtggaaatg ttcgtcgggg ttggcgcatc
      961 tcgtgtgcgc gatatgttcg agcaggccaa gaagaacgcg ccgtgcatca tcttcatcga
     1021 cgaaatcgac gcggtcgggc gccatcgcgg cgctggtcac ggcggcggca atgacgagcg
     1081 cgagcagacg ctgaaccagt tgctggtcga gatggacggc ttcgaggcgc aggaaggcat
     1141 catcatcatc gccgccacca accgcccgga cgtgctggac tcggcgctgc tgcgccccgg
     1201 ccgcttcgac cgtcaggtga cggtgccgaa ccccgacctg accgggcgcg aggcgatcct
     1261 gcgcgtccac atgaagcagg tgccgctggc cgtcgacgtc gacgtcaagg tgatcgcgcg
     1321 cggcacgccg ggcttttccg gcgccgacct cgccaacctc gtcaacgagg cggccctgat
     1381 ggcggcgcgc aaggaccgca agctggtcac catgcgcgac ttcgaggacg ccaaggacaa
     1441 ggtgatgatg ggcgccgagc gccgctcgat ggccatgagc gaggacgaga agaagctgac
     1501 cgcctatcac gagggcggcc acgccatcgt cgcgctcaag gcgccgcagg ccgatccggt
     1561 gcataaggcg accatcatcc cgcgcggccg cgcgctgggc atggtcatgc aactgcccga
     1621 aggcgaccgc tattcgcaga acaagatcca gatggagtcg cgtctggcca tcctgatggg
     1681 cgggcgcgtg gccgaggagc tgatcttcgg caaggacgcc gtgacctccg gcgcctcgtc
     1741 cgacattcag caggcgacca agctggccaa ggcgatggtc acgcgctggg gctattcgga
     1801 cgagctgggc ctcgtcaact accgcgattc cgaggacgag tacggcgcct atgcgcgcga
     1861 cgtgtccgag gagacctcgc gcaagatcga cgccgagatc aaggcgctgg tgcagaaggg
     1921 ctatgacgag gccaagcgca tcctgaccga atataatgac gggctgcacc gtctggccag
     1981 gacgctgctc gaactcgaaa ccctgaccgg ggacgagatc gccaaggtgc tgaacggcga
     2041 ggcgatcgag cgcgacgagg atacgggcgt cgccatcgaa ctcagctcga actcggcggt
     2101 gccggagacg gagacgccga aggccgagcc gagcattgtg ccggtggtcg ccgagtagta
     2161 gaatcaggcc
//
LOCUS       DASVLL010000239         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_13903, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000239 DASVLL010000000
VERSION     DASVLL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13903"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF402_03920"
     CDS             <1..460
                     /locus_tag="VF402_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799471.1"
                     /translation="NDNGRRIPLRIAPDGRITPLPTRAQLNGGATITTVRPESCSVAM
                     KLKVTTTQGLKTSYDAQGLAQGADQGNRAMSKVAGILAIGLAKLDRVYFIGGDDAVAI
                     LGNGQKQPLPRTAGKGEYPAGTPYYVPAQLRGATRIELSHTPSNVLYDTN"
     gene            complement(465..962)
                     /locus_tag="VF402_03925"
     CDS             complement(465..962)
                     /locus_tag="VF402_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799472.1"
                     /translation="MKSSALSRFQDWLSEWSDAKTRQVLVVVLGVLCGLLLGMVVYAA
                     AKYALDQMFPFPPGLYMAHGDEKAALMKTVAVQLFIVIPLTWAVGALAGGYCAARMGR
                     AGQFPAWIAGILLVAYDWIDLLSLPNDAALFVLCPVVVGLCAFAGGWLGMYVEARKLM
                     SSVAE"
     gene            complement(1026..1451)
                     /locus_tag="VF402_03930"
     CDS             complement(1026..1451)
                     /locus_tag="VF402_03930"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7799473.1"
                     /translation="MAKSASDPSELPDPADLGILTTAMPSDTNPSGDIFGGWLMSQMD
                     LAAGNVAARYSQGRVATIAVDGMVFLTPVQVGDEVTVYAKMLSVGRTSMRMKVDAYRR
                     PREGEQKTRVTEAIFTFVAIDEAGRPRPVRPQAKDSPAD"
     gene            complement(1571..1813)
                     /gene="rpsU"
                     /locus_tag="VF402_03935"
     CDS             complement(1571..1813)
                     /gene="rpsU"
                     /locus_tag="VF402_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7799474.1"
                     /translation="MNGEIPLVQIFVRDNNVDQALKALKKKMQREGSFREMKRHVHYE
                     KPSEKRARQQAEAVRRARKLARKRMQREGGLTGRTK"
     gene            2048..>2518
                     /locus_tag="VF402_03940"
     CDS             2048..>2518
                     /locus_tag="VF402_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEX7799475.1"
                     /translation="MRSMLRRMTDAASPLRAAERQLALAAADSIPSLGLNRHSVLAGA
                     RKAGLSEAEADLIAPNGAADIAAVLWRLHDEVLDAPETAESLAGMKVRDRIGYLLNLR
                     LDTAAAQPELARRLMGFFALPTHAALYHKLLWATADRIWKLAGDQALDENHYSKR"
BASE COUNT          455 a          867 c          810 g          386 t
ORIGIN      
        1 caacgacaac ggccgccgca tcccgctgcg catcgcaccg gacggccgca tcacgccgct
       61 gccgacccgc gcgcaactca atggcggcgc gacgatcacc accgtgcgcc cggaaagctg
      121 ctcggtggcc atgaagctca aggtcaccac gacgcagggg ttgaaaacca gctatgacgc
      181 gcagggcctg gcgcagggcg ccgatcaggg caaccgcgcc atgagcaagg tcgccggcat
      241 cctggccatc ggcttggcca agctggaccg cgtctatttc atcggcggcg acgacgccgt
      301 ggcgatcctc ggaaacggcc agaagcagcc tctgccgcgc acggcgggca agggcgaata
      361 tccggcgggc acgccttatt acgtccccgc gcaactgcgc ggcgcgacgc gcatcgagct
      421 gtcgcacacg ccgtcgaacg tgctttacga cacgaactga cgcgctattc ggcgacgctg
      481 ctcattagct tgcgcgcctc gacatacatg cccagccagc cgcccgcgaa ggcgcacagg
      541 ccgaccacca ccgggcacag cacgaacagc gccgcgtcgt tgggcaggct cagcaggtcg
      601 atccagtcat aggcgaccag caggatgccc gcgatccacg ccggaaactg gcccgcccgg
      661 cccatgcgcg cggcgcaata gcccccggcc agcgccccga ccgcccaggt cagcggaatg
      721 acgatgaaca gttgcacggc caccgtcttc atcagcgcgg ccttctcgtc gccgtgcgcc
      781 atgtagaggc ccggcgggaa cgggaacatc tggtccagcg cgtacttggc ggcggcgtac
      841 accaccatgc ccagcaacag cccgcacagc acgcccagca ccaccaccag aacctgtcgc
      901 gtcttcgcgt cgctccattc gctgagccag tcctgaaaac ggctgagagc ggaagatttc
      961 atggcgtgtc ccctgcggag atcggcgtct gcgcgcgccg gcgatcgcgt cagtattaac
     1021 acaatttaat ccgcgggcga gtcctttgct tgcggacgca ccggacgcgg gcggcccgcc
     1081 tcgtcgatgg cgacgaaggt gaagatggcc tcggtcacgc gggtcttctg ctcgccctcg
     1141 cgcgggcggc ggtaggcgtc gaccttcatg cgcatcgagg tgcgcccgac cgacagcatt
     1201 ttcgcgtaca ccgtcacctc gtcgccgacc tgcaccggcg tcaggaacac catgccgtcc
     1261 accgcgatgg tggcgacgcg gccctgagaa tagcgggcgg cgacgttgcc cgccgcaagg
     1321 tccatctgcg acatcagcca gccgccgaaa atatcgcccg acgggttggt gtcggacggc
     1381 atggccgtgg tcaggatgcc cagatcggcg ggatcgggca gttcggaagg atcggatgcg
     1441 gatttggcca tgtgacgccc ccagacgacg caaaacaaag atgacgcgga acaaaagaaa
     1501 acgccgggac agccatgtgt cccggcgccg atgacaaatc ttcagagcgc ggcgcgcatc
     1561 tgcgtccgtc ttacttcgtg cggcccgtca ggccgccttc gcgctgcatg cgcttgcggg
     1621 ccagcttgcg ggcgcgacgc acggcttccg cctgctggcg ggcgcgcttc tcggacggct
     1681 tttcgtagtg gacgtggcgc ttcatctcac ggaaggagcc ttcgcgctgc attttcttct
     1741 tcagggcctt cagggcctga tcgacattgt tgtcgcggac gaaaatctgt accagaggta
     1801 tctctccatt cagggtcgaa ggcgggagga ggaacaggcc ttgcgccgcc gttcattccg
     1861 ccgaaagtga ataaaaacct gtccccgcca acaggagctg gcgggaaaat cgggactgcc
     1921 atacatgagc cgcgccgcca tgtccagatc agccccgcgt gaaaagggaa tttcggcgcg
     1981 atttttaccg aaccggcgcc ggattgcggc tttatgcggc gcaaagcggc gcttggcggc
     2041 gcgcgcgatg cggtctatgc tgcggcgcat gaccgacgcc gcctccccgc tcagggccgc
     2101 cgaacgccag ttggcgctgg ccgccgccga ttccatcccg tcgctgggcc tgaaccgcca
     2161 cagcgtgctg gccggcgcgc gcaaggccgg gctgtccgag gccgaggccg acctgatcgc
     2221 gcccaacggc gccgccgata tcgccgccgt gctctggcgg ctgcacgacg aagttctgga
     2281 cgcgccggaa acggccgaat cgctggcggg catgaaggtg cgcgaccgga tcggctatct
     2341 tctcaacctg cgcctcgata cagcagcggc tcagcccgaa ctggcgcggc gcctgatggg
     2401 cttcttcgcc ctgccgacgc atgcggcctt gtaccacaag cttctgtggg cgacggcgga
     2461 ccgcatctgg aagctggcgg gcgatcaggc gctcgacgaa aaccactatt ccaagcgg
//
LOCUS       DASVLL010000240         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_13950, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000240 DASVLL010000000
VERSION     DASVLL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13950"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VF402_03945"
     CDS             complement(<1..412)
                     /locus_tag="VF402_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7799476.1"
                     /translation="MMTVLQSSLIQVMVGAVRKACKGVARDFGEVSELQVSKKGPGDF
                     VTAADKRVEAALFEELMRVRPGYGFLGEEQGEVAGTDKTHRWIVDPIDGTTNFMHGIP
                     HFACTVALERDGEIVAGVTYNPVANDLYWAEKGKG"
     gene            complement(583..1086)
                     /gene="efp"
                     /locus_tag="VF402_03950"
     CDS             complement(583..1086)
                     /gene="efp"
                     /locus_tag="VF402_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX7799477.1"
                     /translation="MKINANTIKPGKGGAFAQVEMKNLVTGTKLNERFRSDDTVERVT
                     LEQRDHSFLYEDGDMLVFMDTENYEQISLSKEWVGEDQVRFLHDGMLVVIESHEEKPI
                     GMSLPDTVVLEVVETEPTIKGQTASSSYKPAKASNGMRVMIPPFMGVGERIVVSTETG
                     EYMKRAD"
     gene            1239..2210
                     /locus_tag="VF402_03955"
     CDS             1239..2210
                     /locus_tag="VF402_03955"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7799478.1"
                     /translation="MRHIVRHISRRETLLVGLAAGAAASLPALARADATPRAVTPPGM
                     ILGEGPIWSARDNALYWVDIRGKKIHRLNFKDDSQQSWDTPEIVPWIVERKSGGFLCA
                     IVRTVYAVSFDPEFKLAPLFSIDGDDKAVRLNDAKVDPMGRLWTGTMDLPFTDRVAKL
                     YRVDADLSVHTMDTGYICTNGPAFSADGKTLYHNETHDGIVYAFDLAPDGSLSHKRVF
                     VKFPEGVGFPDGCTTDAQGGLWVSHYGGARVSRYTPDGKLDFDVPVPAKQSTSLTFAG
                     PNLDRLFVTTAAQPPVDADDKLAGTLFEIPSSSLRGHTGLPMGVFAG"
     gene            complement(2207..>2754)
                     /locus_tag="VF402_03960"
     CDS             complement(2207..>2754)
                     /locus_tag="VF402_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7799479.1"
                     /translation="PDVILLDLGLPDMDGMDVIRRVRDWGATPIVVLSARDRERDKVA
                     ALELGADDYLTKPFSAAELIARIRVAQRHAGRRGGDAAFVFDQGGLFIDSAARRVTLD
                     GAEVRLTPTEYRLLLTLARHADKVLPHSTLLAEVWGKHGQDTQTYLRIHTQHLREKLK
                     DDPLKPRFIVTEPGIGYRLRA"
BASE COUNT          458 a          941 c          876 g          479 t
ORIGIN      
        1 cgcccttgcc cttttcggcc cagtagaggt cgttggcgac cggattatag gtcacgcctg
       61 ccacgatctc gccgtcgcgc tccagggcca ccgtgcaggc gaagtgcgga atgccgtgca
      121 tgaagttggt ggtgccgtcg atcggatcga cgatccagcg gtgcgtcttg tcggtgcccg
      181 cgacctcgcc ctgctcctcg ccgaggaagc cgtagccggg acgcacgcgc atcagttcct
      241 cgaacagggc ggcctcgacg cgcttgtcgg cagcggtgac gaaatcgccc ggccccttct
      301 tcgagacctg caattcggac acctcgccga aatcgcgcgc cacgcccttg caggccttgc
      361 gcaccgcgcc gaccattacc tgaatgaggg aggattgcag aacagtcatc atgaaaccta
      421 tctatcctcc cctgcgacag cgggggaggg ggaccgcgaa gcggtagagg gggcgtcctc
      481 gcggatcccc tctccatcat ctcgcattgc tcgacgccgc ctcccccact tttcgtcgtg
      541 ctaacgcttc gctgcctgag cacgatgcag gggaggatta atttagtcag ctctcttcat
      601 gtattcgccg gtctcggtcg agacgacgat gcgctcgccc acgcccatga agggcgggat
      661 catgacgcgc atgccgttgg aggccttggc gggcttgtag gaggacgagg cggtctggcc
      721 cttgatggtc ggctcggtct cgacgacctc cagcaccacc gtgtcgggca ggctcatgcc
      781 gatcggcttt tcctcgtgcg attcgatgac cacaagcatg ccgtcgtgca ggaagcggac
      841 ctgatcctcg ccgacccatt ccttggaaag gctgatctgc tcgtaattct cggtatccat
      901 gaacaccagc atgtcgccgt cctcgtacag gaacgaatgg tcgcgctgct ccagcgtgac
      961 gcgctcgacc gtgtcgtcgg aacggaagcg ctcgttcagc ttggtgccgg tgacgaggtt
     1021 cttcatctcg acctgggcga aggcgccgcc cttgccgggc ttgatcgtat tggcgttgat
     1081 tttcatgaga tacgacttgt caaaaaaggg gtggttcggc gcgttactac ccggatcggc
     1141 gcggaaaggc aaggggcggc gccagattgc aaacgcttga cgcgccacgc gtctgcgggc
     1201 cttatgcgcg gcataaaacc ataatgagag gatacgtcat gcgtcacatc gtccgtcaca
     1261 tcagccgccg tgaaaccctg ctcgtcggtc tcgccgccgg cgcggccgca agcctgcccg
     1321 ccctggcgcg cgccgacgcg acgccgcgcg ccgtcacgcc gccgggcatg atcctgggcg
     1381 aaggcccgat ctggtcggcg cgcgacaacg cgctctactg ggtcgacatc cgcggcaaga
     1441 agatccaccg cctgaatttc aaggacgaca gccagcaaag ctgggacacg cccgaaatcg
     1501 taccgtggat cgtcgagcgc aaatcgggcg gctttctgtg cgccatcgtg cgcaccgtct
     1561 acgccgtcag cttcgacccg gaattcaaac tggcgccgct gttcagcatc gacggcgacg
     1621 acaaggccgt gcggctcaac gacgccaagg tcgatccgat ggggcgtctg tggaccggca
     1681 cgatggacct gcccttcacc gaccgcgtcg cgaagctcta ccgcgtcgat gcggacctga
     1741 gcgttcacac gatggacacc ggctatatct gcaccaacgg cccggccttc tcggcggacg
     1801 gcaagacgct ctatcacaac gagacgcacg acggcatcgt gtacgccttc gacctggccc
     1861 ccgacggctc gctcagccac aagcgcgtgt tcgtcaagtt ccccgaaggc gtcggcttcc
     1921 ccgacggctg caccaccgac gcgcagggcg gcctgtgggt gtcgcactac ggcggcgcgc
     1981 gcgtcagccg ctatacgccc gacggcaagc tcgacttcga cgtccccgtc ccggccaagc
     2041 aatcgaccag cctgaccttc gccggtccga acctcgaccg cctgttcgtc accaccgccg
     2101 cccagccgcc ggtggacgcc gacgacaagc tggccggaac cctgttcgag attccgtcca
     2161 gttccttacg cggccacacc ggcctgccga tgggcgtgtt cgcgggctag gcccgcaggc
     2221 ggtagccgat tccgggctcc gtgacgatga agcgcggctt gagcggatcg tccttcagct
     2281 tctcgcgcag gtgctgggtg tggatgcgca gataggtctg cgtgtcctgc ccgtgcttgc
     2341 cccagacctc ggccagcagg gtcgaatgcg gcagcacctt gtcggcgtgg cgcgccaggg
     2401 tgagcagcag cctgtattcc gtcggggtca ggcgcacctc ggcgccgtca agcgtcaccc
     2461 tgcgggcggc gctgtcgatg aacaggccgc cctgatcgaa gacgaaggcg gcgtcgccgc
     2521 cgcgccgccc ggcgtggcgc tgcgccaccc ggatgcgcgc gatcagctcc gcggcggaga
     2581 acggcttggt caggtaatcg tccgccccca gttcgagcgc ggcgaccttg tcgcgctcgc
     2641 ggtcgcgcgc cgacaggacc acgatcggcg tcgcgcccca gtcgcgaacg cggcggatga
     2701 cgtccatgcc gtccatgtcg ggcaggccca gatcgagcag gatcacgtcc gggc
//
LOCUS       DASVLL010000241         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_14035, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000241 DASVLL010000000
VERSION     DASVLL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14035"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1293)
                     /locus_tag="VF402_03965"
     CDS             complement(97..1293)
                     /locus_tag="VF402_03965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX7799480.1"
                     /translation="MKPLDALRRMDGLNAERARLYSRFGAAGFAILAGLIWLKFAVLS
                     PGAEPLGGDFVSFYAAGKLALSGHAADAWRPALHAAAEDGLFHGAHGYLAFFYPPPYL
                     LVCTPFALLPYGWAALIWMAATTMLGLWAVRAALNRRLSFAALLAFPAIWIDIGCGQN
                     GALSLAILSAGFALMPRRPILGGLVLGCMVIKPQLAVALPFVLGGAALARPRYWTTFI
                     ATGAGALILCGLSWLAFGAEGWRAFLANSVYARETLHRGLVDPALMQSLFAGLRVLGV
                     PFTPAFAAQGLLSLGVLGAAAYLSWRHRLDGAALGALAVSATLLATPFLLDYDLVILA
                     LPLGWLCVEGARKGFRDWEKLWLLAAFFLPLVSRKLAQAAHLPVAPPMLLVVFVLLAA
                     RLRDRR"
     gene            complement(1290..2483)
                     /locus_tag="VF402_03970"
     CDS             complement(1290..2483)
                     /locus_tag="VF402_03970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX7799481.1"
                     /translation="MPARLLTFLRDARWLNAERVRGYSLILLGVSAAGFLGWIALSHG
                     LIDRAGKPLGTDFMSFYAAGKLALSGRAAEAWSPAAHHAAENAIFNRDLGYWAFFYPP
                     AYLLVCAPLALAPYGAALLLWLGATAAAMLVLVRRWLRGLADAPVWLPLLAFPALWID
                     LGNGQNAALTTAIFAGGALLLPKRPWLAGAVFGLLIIKPQLGLALPVLLAASGRWRTF
                     AVAGLSAAALSLAALPLVGAEGYLAFLRNAPLARATLEQGLVPPSAMVSVFAAVRTLG
                     GGAALAYAAQGACALTVLGLAALIVRRHRPDPAAFCALLAAASLLISPFLLDYDLLLA
                     AVPLGWLALQGVRHGFRPWEKLLLLCAALTPLLARPLAAHLHLPVAPVALLALFALIA
                     RRITA"
BASE COUNT          478 a          894 c         1020 g          253 t
ORIGIN      
        1 gagagcgacg ccgatttcag ccctctgcgc atcgtggccg acgccatgct gctgggccgc
       61 cggatcgacg cgccggacta tatcgaatag ggcggctcag cgtcgatccc ggaggcgggc
      121 ggccagcagg acgaacacga ccagcagcat cggcggcgcg accggcaggt gcgcggcctg
      181 cgccagcttg cgcgagacga gcggcaggaa gaaggccgcc agcagccaca gcttttccca
      241 gtcgcggaaa ccttttcgcg cgccctcgac gcacagccag cccagcggca gggccaggat
      301 gacgagatcg tagtcgagca ggaacggcgt cgccagcagg gtggccgaga cggcgagcgc
      361 gccgagcgcc gcgccgtcga ggcgatgacg ccagctcaga taggccgccg cgccgaggac
      421 gccgagcgac agcaggccct gcgcggcgaa ggcgggcgtg aacgggacgc cgagcacgcg
      481 cagtccggcg aacaggctct gcattagggc cggatcgacc agaccgcgat gcagggtttc
      541 gcgcgcatag acgctgttgg cgaggaaggc gcgccacccc tcggcgccga aggccagcca
      601 tgacagaccg cacaggatga gcgcgcccgc cccggtggcg atgaaggtcg tccagtagcg
      661 cggccgggcc agcgcggcgc cgccgaggac gaagggcagg gcgacggcca gttgcggctt
      721 gatcaccatg cagccgagaa cgaggccgcc caggatcgga cggcgcggca tgagcgcgaa
      781 accggcgctc aggatcgcca gcgacagggc gccgttctgg ccgcagccga tatcgatcca
      841 gatcgccgga aaggccagca gggcggcgaa cgaaaggcgc cggttcagcg ccgcgcggac
      901 cgcccacagg ccgagcatgg tcgtcgccgc catccagatg agcgccgccc agccataggg
      961 caacagggcg aacggcgtac agaccagcag gtagggcggc gggtagaaga aggcgagata
     1021 gccatgcgcg ccgtggaaca ggccgtcctc ggcggcggcg tgcagggcgg gacgccaggc
     1081 gtcggcggcg tggccgctca gggccagctt cccggcggcg tagaagctga cgaaatcgcc
     1141 gcccagcggc tcggcgccgg ggctgaggac cgcaaacttc agccagatca gtccggccag
     1201 aatggcgaac ccggcggcgc cgaagcggct gtagaggcgc gcgcgttcgg cgttgaggcc
     1261 atccatgcgg cgcagggcgt cgagcggctt catgcggtga tccggcgggc gatcagggcg
     1321 aacagggcca gcagcgccac cggcgcgacg ggcaggtgca ggtgggcggc cagcggccgc
     1381 gccaatagcg gcgtcagcgc ggcgcacagc agcagcagct tctcccacgg gcggaaaccg
     1441 tgcctgacgc cttgcagggc cagccagccg agcggaacgg cggccagcaa aaggtcgtag
     1501 tcgagcagga acggactgat cagcagggac gcggcggcca gcagcgcgca gaaggcggcg
     1561 gggtccgggc gatgacggcg cacgataagg gcggcgaggc cgagaaccgt cagcgcgcac
     1621 gcgccttgcg cggcgtaggc gagggcggcg ccgccgccca gcgtcctgac cgccgcgaaa
     1681 acgctgacca tggcggacgg cggcacgagg ccctgctcca gcgtggcgcg cgccagcggg
     1741 gcgttgcgca ggaaggcgag atagccctcg gcgccgacca gcggcagggc ggccaggctg
     1801 agcgcggcgg cgctcagacc ggcgacggcg aaagtcctcc agcggcccga cgccgccagc
     1861 agcacgggca gggccaggcc aagctgcggc ttgattatca gcaggccgaa caccgcgccc
     1921 gccagccacg ggcgcttcgg cagcagcagc gcgccgccgg cgaagatcgc cgtggtcagg
     1981 gcggcgttct ggccgttgcc gaggtcgatc cacaaggccg gaaaggccag cagcggcagc
     2041 cagaccggcg cgtcggcgag gccgcgcagc cagcgccgga cgaggaccag catggccgcc
     2101 gccgtcgcgc ccagccagag caggagcgcc gcgccgtaag gcgccagcgc cagcggcgcg
     2161 cagaccagca ggtaggcggg cggatagaag aaggcccagt agccgaggtc gcggttgaag
     2221 atggcgtttt ccgcggcgtg gtgcgcggcg ggcgaccagg cttcggcggc gcggccgctc
     2281 agggccagct ttccggcggc gtagaagctc atgaaatccg tgccgagcgg cttgcccgcg
     2341 cggtcgatca ggccatgcga cagggcgatc cagccgagga agcccgccgc gctgacgccg
     2401 agcaggatca ggctgtagcc gcgcacgcgc tcggcgttca gccagcgcgc gtcgcgcagg
     2461 aaggtcagca ggcgtgccgg caaaacaggc tccgaacgga tgacatcccc ctctcccctc
     2521 catgagggga gaggacgaga gccgagcgaa agcgaaggcg atcgggtgag gggctgcgcg
     2581 caagcgtcgg gcggcccctc acccgaggat cggcggcttc gccttgctcc tcgtcctctc
     2641 cccgc
//
LOCUS       DASVLL010000242         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_14073, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000242 DASVLL010000000
VERSION     DASVLL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14073"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF402_03975"
     CDS             complement(<1..401)
                     /locus_tag="VF402_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799482.1"
                     /translation="MSLRHTLLLAAAVALAPVASHAQDLMSRSLTAPAADQWQSSGNG
                     ESHKLVKDDGVRGGWAYQVTAAKAGQNPWDVQANVSTDRPVKNGDVILLAFWAKAATP
                     PAGAAAISAPAAIQEAAAPYTRLGEANLSITP"
     gene            complement(538..>2552)
                     /locus_tag="VF402_03980"
     CDS             complement(538..>2552)
                     /locus_tag="VF402_03980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7799483.1"
                     /translation="PLQAPPFDRIKDTDYQPAIEEGMRQQLAEIDAIANDPAPPTFEN
                     TLVAMEKSGRMLDRVTSAFYAVVQANTNPTLDKVQTDEAPRLAAQSDAIYLNAKLFQR
                     VKALYDARDSLKLDPESRRLLEVKYRQFVHAGANLSDADKARLRQINQQDASLETEFQ
                     QRLIAGSKAAAFVTADKADLAGLSDAEIDAAAKAAQDRNLPGQYLIPQQNTTQQPVLA
                     ELTDRAVREKIYDNSITRNEKGDGNDTRAIIRQLAQLRAEKAGLLGYPNYAAYVLYDQ
                     MAQTPEAVESFINKLVPATAARIADESRQIQAMIDRSGPHFDLQPWDWEMYSDKVRKA
                     KYDLDQNALKPYFELNKVLQDGVFYAANRLYGVTFKERHDLPVYQPDVRVFDVFDKDG
                     SQLGLIYFDYFKRDNKAGGAWMSNFVNQSHLLGTKPVVYNVANFAKPAPGQPALISFD
                     DVTTMFHEFGHALHGLFADSRYASLSGANTARDWVEFPSQFNEHWALYPDVLKHYAVD
                     YRTGEPIPQALVDKIKASATWGQGYELGELLAASDLDMQWHMLPADAPVQDVDAFETK
                     ALAETHTDFKNVPPRYRSTYFLHIWANGYAAGYYAYQWTVMLDDDAFAWFEKNGGLTR
                     ANGQRFRDMILSKGQTEDYGDMFRAFYGKDPDIGPMLEHRGLTAPK"
BASE COUNT          381 a          781 c          882 g          508 t
ORIGIN      
        1 ggcgtgatgc tgagattggc ctcgcccagc cgcgtatagg gggcggcggc ctcctgaatc
       61 gcggcggggg cgctgatcgc cgccgcgccc gcgggcgggg tcgcggcctt ggcccagaag
      121 gccagcagga tcacgtcgcc gttcttgacc ggccggtcgg tgctgacgtt cgcctgcacg
      181 tcccacgggt tctgcccggc cttggccgcc gtcacctgat aggcccagcc gccgcgcacg
      241 ccgtcgtcct tgaccagctt gtgactctcg ccattgccgg aggattgcca ctgatccgcc
      301 gcgggcgcgg tcagcgagcg cgacatcagg tcttgggcat gcgacgcgac aggcgccagc
      361 gcgacggcgg cggcgagcaa cagggtgtga cgaagcgaca tggcggcctc ccataattat
      421 gtgatccgcc tatgtttcag gccgggaggg aggcgtcaag tcatgtcggg tcgcgcggtg
      481 aaggaaaacc ctccggcgcg cggacgccgg agggtttcat cttgcgtccg atacgcccta
      541 tttcggcgcc gtcaggccgc gatgttcgag catcgggccg atgtccgggt ctttgccgta
      601 aaaggcgcgg aacatgtcgc catagtcttc ggtctggccc ttggacagga tcatgtcgcg
      661 gaagcgctgg ccgttggcgc gagtcaggcc gccgttcttc tcgaaccagg cgaaggcgtc
      721 gtcgtccagc atcaccgtcc actgataggc gtaatagccc gccgcgtagc cgttggccca
      781 gatgtgcagg aaataggtcg agcgatagcg cggcggcacg ttcttgaagt cggtatgggt
      841 ctcggccagc gctttggtct cgaacgcgtc gacgtcctga acgggcgcgt cggcgggcag
      901 catgtgccat tgcatgtcga ggtccgaggc ggccagaagt tcgcccagct cgtagccctg
      961 cccccaggtc gcggacgcct tgatcttgtc gaccagggcc tgcggaatcg gttcgccggt
     1021 cctgtaatcg acggcgtaat gcttgaggac gtcgggatag agcgcccagt gttcgttgaa
     1081 ctgcgacggg aactcgaccc agtcgcgcgc cgtgttggcg ccggacaggc tggcgtaccg
     1141 gctgtcggcg aacaggccgt gcagggcgtg gccgaattcg tggaacatgg tcgtcacgtc
     1201 gtcgaagctg atcagggcgg gctggcccgg cgcgggcttg gcgaagttgg cgacattata
     1261 gacgaccggc ttcgtgccca ggaggtgcga ctgattgacg aaattgctca tccaggcgcc
     1321 gcccgccttg ttgtcgcgct tgaagtagtc gaaatagatc aggcccagtt gcgagccgtc
     1381 cttgtcgaac acgtcgaaca cgcgcacgtc cggctgatag accggcaggt catggcgctc
     1441 cttgaaggtg acgccgtaaa gccggttggc ggcgtaaaag acgccgtcct gcaacacctt
     1501 gttcagctcg aaatagggct tcaacgcgtt ctggtcgaga tcgtatttcg ccttgcgcac
     1561 cttgtcggaa tacatttccc agtcccaggg ctgcaaatcg aagtgcgggc cggaccggtc
     1621 gatcatcgcc tgaatctgcc ggctttcgtc ggcgatcctg gcggcggtgg cgggaaccag
     1681 tttgttgatg aagctttcga ccgcctccgg cgtctgcgcc atctggtcgt acagcacata
     1741 ggcggcgtag ttgggatagc cgagcagccc ggccttttcg gcgcgcaact gcgccagttg
     1801 ccggatgatg gcgcgcgtgt cgttgccgtc gcccttttcg ttgcgcgtga tcgagttgtc
     1861 gtagatcttt tcacgcacgg cgcggtcggt cagttcggcc agcaccggct gctgcgtcgt
     1921 gttctgctgc gggatcaggt actggccggg caggttgcgg tcctgcgcgg ccttggcggc
     1981 ggcgtcgatc tccgcgtcgc taagcccggc caggtcggcc ttgtcggcgg tgacgaaggc
     2041 ggcggccttc gacccggcga tcagcctttg ctggaactcg gtttccagcg aggcgtcctg
     2101 ctggttgatc tggcgcagcc tggccttgtc ggcgtcggac aggttggcgc ccgcatgcac
     2161 gaactgccgg tacttgacct ccagaagccg ccgcgattcc gggtccagct tcagcgaatc
     2221 gcgcgcgtca tagagcgcct tgacgcgctg gaacagcttt gcgttcaggt agatggcgtc
     2281 gctctgcgcg gccaggcgcg gcgcctcgtc cgtctgcacc ttgtcgagcg tcggattggt
     2341 gttggcctgc acgacggcgt agaaggcgga ggtgacgcgg tcgagcatcc gcccggattt
     2401 ttccatcgcc accagcgtgt tctcgaaggt cggcggcgcc ggatcgttgg cgatggcgtc
     2461 gatttcggcc agttgctgtc tcatgccctc ctcgatggcg ggctggtaat ccgtgtcctt
     2521 gatcctgtcg aacggcggcg cctgcaaggg ca
//
LOCUS       DASVLL010000243         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_389111,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000243 DASVLL010000000
VERSION     DASVLL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389111"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VF402_03985"
     CDS             complement(<1..449)
                     /locus_tag="VF402_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX7799484.1"
                     /translation="MKKTQDELVFLPLGGSNEIGMNLNLYGFGPADNRQWIIVDVGVT
                     FGDLTTPGIEVIVPDPEFLKGEKILGIVLTHAHEDHIGAIGWLWPRLKAPLYATPFTA
                     YLIREKLRDANLLDQVVINEVPLSGRFTLGPFELELVTITHSIPEPNG"
     gene            complement(471..1241)
                     /locus_tag="VF402_03990"
     CDS             complement(471..1241)
                     /locus_tag="VF402_03990"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX7799485.1"
                     /translation="MLLAIEQGNTNTLFAIHDGSEWVAQWRVATESTRTADEYAAWLY
                     QLMSMRGLDFAAIDDCIISSVVPQSLFNLRKLAQRYFGKTPYVIGENTRLGIDVRIDK
                     PKEAGADRLVNAVGGHVAYDGPLLIIDSGTATTFDVVAADGGYEGGAIAPGVNLSLQA
                     LHAAAAKLPRIAIERPARYIGKETVSAMQSGIFWGYIGLIEHLVGGIQAEYGQKMTVI
                     ATGGVASLFEGATDRIDYFDHDLTLRGLLEVYRRTLAA"
     gene            complement(1225..1983)
                     /locus_tag="VF402_03995"
     CDS             complement(1225..1983)
                     /locus_tag="VF402_03995"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7799486.1"
                     /translation="MARFEIFDSLPSTQSLALQRLRAGDGEAGWILAREQTKGVGQHG
                     RSWVGQKGNFMASRYEVMDLDVRHAPQLSFVTALAVYEMLRPLVPDTDAAMRIKWPND
                     ILHKGRKLCGILVQTEAVEGEGRLGVVIGVGLNVRMAPILEGYPTVALREIGAEAGRI
                     DPEALLYKLNGHLDGVIDLWRRKAFADIAHAWFKRAYGADRRVTAPSEGQPVSGRILG
                     LDGFGALRIEGEDGQVRTISGGEVSYGGPLVTGD"
BASE COUNT          366 a          691 c          656 g          376 t
ORIGIN      
        1 ccgttgggtt cagggatgga gtgggtgatg gtcaccagtt cgagctcgaa ggggcccagg
       61 gtgaagcgtc cggaaagcgg cacctcgttg atgaccacct ggtcgagcag gttggcgtcg
      121 cgcagtttct cgcggatcag ataggcggtg aagggggtgg cgtagagcgg cgccttgagc
      181 ctgggccaca gccagccgat ggcgccgata tggtcttcgt gcgcgtgggt cagcacgatg
      241 ccgaggatct tttcgccctt gaggaattcg ggatcgggca cgatgacctc gatgcccggc
      301 gtggtcaggt cgccgaaggt caccccgacg tccacaatga tccattggcg gttgtccgcc
      361 gggccgaacc cgtaaagatt gaggttcatg ccgatttcgt tcgagccgcc cagcggcaaa
      421 aacaccagtt cgtcttgcgt ctttttcatt cttttccgtt accggcgcct ttaagcggcc
      481 agcgtgcgtc tatagacctc cagcagcccc ctcagcgtca ggtcgtggtc aaaataatcg
      541 atcctgtctg tagcaccttc gaacagcgac gccacccctc ccgtggcgat aaccgtcatc
      601 ttttgcccat attcggcctg aattccgccg accagatgct cgatcaggcc gatatagccc
      661 cagaagatgc cggactgcat ggcgctgacc gtctccttgc cgatatagcg cgcgggcctt
      721 tcgatggcga tgcgcggcag cttggccgcc gccgcgtgca gcgcctgcaa agacaggttg
      781 acgcccggcg cgatggcccc gccctcatag ccgccgtccg ccgccaccac gtcgaaggtc
      841 gtcgctgtgc ccgaatcgat gatcagcaaa ggcccgtcat aggcgacatg gccgccgacc
      901 gcgttgacca gccggtccgc gcccgcttcc ttaggcttgt cgatgcggac gtcgatgccg
      961 agccgcgtgt tttcgccgat cacatagggc gtcttgccga aataacgctg cgccagcttg
     1021 cgcaggttga acagggactg cggcacgacc gacgagatga tgcagtcgtc gatggcggcg
     1081 aaatccagcc cgcgcatcga catgagctga tagagccacg cggcgtattc gtccgccgtg
     1141 cgcgtgcttt cggtcgccac gcgccattgc gccacccatt cggagccgtc atggatggcg
     1201 aacaaagtgt tggtgttgcc ctgctcaatc gccagtaaca agcggtcctc catagctgac
     1261 ctcgccgccg ctgatcgtgc ggacctgtcc gtcctcgccc tcgatgcgca gggcgccgaa
     1321 gccgtcgagg ccaaggatgc gcccgctgac cggctggcct tcggacggcg ccgtgacgcg
     1381 gcggtcggcg ccgtaggccc gcttgaacca cgcatgcgcg atatcggcga aagccttgcg
     1441 ccgccacagg tcgatcacgc catcgagatg gccgttcaac ttataaagca gggcttcggg
     1501 gtcgatccgg cccgcctcgg cgccgatttc gcgcagcgcc acggtcggat agccttccag
     1561 gatcggcgcc atgcgcacat tgaggccgac gccgatcacc acgcccagcc ggccctcgcc
     1621 ttcgaccgcc tcggtctgca ccagaatacc gcacagcttg cgccccttgt gcaggatgtc
     1681 gttcggccac ttgatgcgca tggcggcgtc ggtgtcgggg accagaggcc gcagcatctc
     1741 atagacggcc agagcggtca cgaacgacaa ttgcggcgcg tgacgcacgt cgaggtccat
     1801 gacctcatag cgcgacgcca tgaaattgcc cttctggccg acccaggagc ggccatgctg
     1861 gccgacgccc ttcgtctgtt cgcgggccag aatccagccc gcctccccat ccccagcgcg
     1921 cagcctttgc agggccaggc tctgggtgga aggcaggctg tcgaaaatct cgaaccgggc
     1981 cacggttccg cgactttaca ggccgaagct ttgcgcggcg ttctgcgcca ccggccaggc
     2041 caggatcaga aagacgatga ccgccgggaa ggagaaggcc gccgccgca
//
LOCUS       DASVLL010000244         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_283602,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000244 DASVLL010000000
VERSION     DASVLL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283602"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VF402_04000"
     CDS             <1..331
                     /locus_tag="VF402_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799487.1"
                     /translation="LGVLGAAAYLSWRHRLDGAALGALGVAAALLATPFLLDYDPVIL
                     TLPLGWLCVEGARKGFRDWEKLWLLAAFFLPLVSRKLAQAAHLPVAPPMLLVVFVLLA
                     ARLRDRR"
     gene            complement(338..1288)
                     /locus_tag="VF402_04005"
     CDS             complement(338..1288)
                     /locus_tag="VF402_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7799488.1"
                     /translation="MGLLSLGRKPLKIGLIGLGKIAVDQHIPSIRANKNLELVAGCSP
                     HARPEGVQAYKDMDAMMSQHPEIEAIAICTPPQIRHEIARQVIDIGRHVFLEKPPAAT
                     LGEAESIKAMAAAKGVSLLASWHSRFAPAVEAAREWIAARELRSVTIHWKENVRQWHP
                     GQRWIFEAGGMGVFDPGINSLSILTRILPDAVMVKKADLHTPVNCQAPIQAELDMLSE
                     SGVPIRAEYDFLQEGVQTWSIFVESKSFEKLTLSMGGAELEIDGKTVVKQKEAEYAGL
                     YKHFYELIRKGESDADFSPLRIVADAMLLGRRIDAPDYIE"
     gene            1582..1779
                     /locus_tag="VF402_04010"
     CDS             1582..1779
                     /locus_tag="VF402_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799489.1"
                     /translation="MIDAHMHFWRIGHNDHTWPVVGLGASWRGWLNWTLGWLSDKPDA
                     VRDAVPGGNAARFYALDRMTA"
     gene            1779..2555
                     /locus_tag="VF402_04015"
     CDS             1779..2555
                     /locus_tag="VF402_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7799490.1"
                     /translation="MPDTEKASAEKTLGKYRAPALEKGLDVLELLAAVKAPMTLSQIS
                     QRLTRSVSELFRMVQVLEARGYVAMSQGGEDFELTNKLFSLGISRGPSQNLLASAMPL
                     MQTLSEETRQACHLAIVSDDRMVVIARVEAPGDLGFSVRVGYQRPLAHSTSGLIIYAF
                     QKPKIREQWRRTLRPALTQDEWATFEAQAERALSQGHFTSPSEYIDGITDLSAPIASD
                     AGVIAAMTMPYIHVHDGLTQDEALAKLKTTAATLSKALGG"
     gene            2595..2891
                     /locus_tag="VF402_04020"
     CDS             2595..2891
                     /locus_tag="VF402_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX7799491.1"
                     /translation="MDPFLLLHPDDNVLVCRRQAAAGETVTLDGDAVALPGAVAVGHK
                     VARRALRPGDKVVKYGAPIGSMTAAAARGAHVHLHNMQSDYIPSHTREATGHDD"
     gene            2881..4098
                     /locus_tag="VF402_04025"
     CDS             2881..4098
                     /locus_tag="VF402_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX7799492.1"
                     /translation="MTTDFPTFEAWLRPDGRKGIRNIVVVGYLVECAHHVARRIVQTS
                     GETDVHLIGFPGCYPNAYAYRMMEALCTHPNVGGAVLVSLGCEGFNRESLKRHIEASG
                     RPVETFVIQQAGGTRATIEAGVAAVNRIRAEAAKTPRATMAVAELVIGTICGGSDGTS
                     GITANPAVGRAFDALGAAGAACIFEETGELVGCERIMASRAITPELGLELERCVQKAE
                     RYYTVMGYGSFAPGNAEGGLTTQEEKSMGAYSKSGSAPILGILKPGDVPPSGGLYLLD
                     VVPDGEPRFGFPNISDNAEIVELIACGAHLILFTTGRGSVVGSAISPVIKVCANPETY
                     RRLPDDMDVDAGRIMEGRADIDTVGDEITDLTLRVAGGEESVSEALGHTEFILTYKSF
                     EPAGPACLPLKRA"
     gene            4113..>4656
                     /locus_tag="VF402_04030"
     CDS             4113..>4656
                     /locus_tag="VF402_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7799493.1"
                     /translation="MGRLSGKTALITAAGQGIGRATAEMFVREGARVIATDIDPAALD
                     IDGAESRRLDVTDPAAVKAIAAEYPEVDVLYNCAGVVHAGTILDCGEDEWNFALSLNV
                     TAQYRMIRAFLPAMIANGGGAIINMSSVASSIKGVPNRFAYGATKAAVIGLTKAVAAD
                     FVTKGIRCNAICPGTVETPSL"
BASE COUNT          756 a         1637 c         1486 g          777 t
ORIGIN      
        1 gctcggcgtc ctcggcgcgg cggcttacct gagctggcgt caccgcctcg acggcgccgc
       61 gctgggcgcg ctcggcgtcg cggccgcgct gctggcgacg ccgttcctgc tcgactacga
      121 tcccgtcatc ctgaccctgc cgctgggctg gctgtgcgtc gagggcgcgc gcaaaggctt
      181 ccgcgactgg gaaaagcttt ggctgctggc ggccttcttc ctgccgctcg tctcgcgcaa
      241 gctggcgcag gccgcgcacc tgcccgtcgc gccgccgatg ctgctggtcg tgttcgtcct
      301 gctggccgcc cgcctgcgcg accggcgctg agccgtccta ttcgatatag tccggcgcgt
      361 cgatgcggcg gcccagcagc atggcgtcgg ccacgatgcg cagggggctg aaatcggcgt
      421 cgctctcgcc cttcctgatc agctcgtaga agtgcttgta aagaccggcg tattcggcct
      481 ccttctgctt caccaccgtc ttgccgtcga tctcaagctc ggccccgccc atcgacaggg
      541 tcagcttctc gaacgatttg gactcgacga agatgctcca ggtctgcacg ccctcctgca
      601 aaaagtcgta ctcggcgcgg atcggcacgc cgctttcgct cagcatgtcc agttcggcct
      661 gaatcggcgc ctggcaattg accggcgtgt gcaggtcggc cttcttgacc atcaccgcgt
      721 cgggcaggat gcgcgtcagg atcgacagcg agttgatgcc cggatcgaac acgcccatgc
      781 cgcccgcctc gaagatccag cgctgacccg gatgccattg ccgcacgttt tccttccagt
      841 ggatggtgac gctgcgcagt tcgcgcgccg cgatccattc gcgcgccgcc tcgaccgccg
      901 gagcgaagcg cgaatgccag ctggccagca gcgacacgcc cttggccgcc gccatcgcct
      961 tgatcgactc ggcctcgccc agcgtcgcgg cgggcggctt ttcgaggaag acgtggcggc
     1021 cgatgtcgat cacctgacgg gcgatctcgt ggcggatctg cggcggggtg cagatggcga
     1081 tggcctcgat ctcgggatgc tggctcatca tcgcgtccat gtccttatag gcctgcacgc
     1141 cttcggggcg cgcgtgcggc gagcagcccg cgaccagttc gagattcttg ttggcgcgga
     1201 tggagggaat atgctggtcc accgcgatct tgccgaggcc gatcaggccg attttcagcg
     1261 gtttgcggcc cagcgagaga agtcccatag ttcgtttcct tatcgtcgat tacggcggtt
     1321 ataaattatc atacaatttg atcaagcgca ttctgaagcc tcagccacgc gcccgccctt
     1381 cattttgtcc gactatttgc cagagccttc atgcgaaagc aatcgccaag cgccccgcag
     1441 ccgtaatttc atatttgacc tatttattca tatgtgatgt gctgacagcg cggcggcgcc
     1501 ggatcgcctt cgatattcgc agggacgcgg aaacccgccg ggcgcaacgg cgcctcgcaa
     1561 gctaaggaaa gccggaacgg catgatcgac gcgcatatgc atttctggcg gatcgggcat
     1621 aacgatcata catggccggt cgtcggcttg ggcgcgtcgt ggcgcggctg gctgaactgg
     1681 acgctgggct ggctatcgga taagccggac gccgtgcgcg acgccgttcc cggcggcaac
     1741 gccgcccgct tctacgccct cgacaggatg accgcctgat gccggatacc gaaaaagcct
     1801 ccgccgaaaa gacgctcggc aaataccgcg cgcccgccct ggaaaagggg ctggatgtcc
     1861 tcgaactgct ggccgccgtc aaggcgccga tgaccctgtc gcagatttcg cagcgcctga
     1921 cgcgctcggt cagcgaactg ttccgcatgg tgcaggtgct ggaggcgcgc ggctacgtcg
     1981 ccatgtcgca gggcggcgag gacttcgagc tgaccaacaa gctgttctcg ctcggcattt
     2041 cgcgcggccc cagccagaac cttctggcca gcgccatgcc gctgatgcag accctgagcg
     2101 aggagacgcg ccaggcctgc cacctcgcca tcgtctccga cgaccgcatg gtggtgatcg
     2161 cgcgcgtcga ggcgcccggc gatctcggct tttcggtgcg cgtcggctat cagcgcccgc
     2221 tggcgcactc gacctccggc ctgatcatct acgccttcca gaagccgaag atccgcgaac
     2281 agtggcggcg gacgctgcgc ccggccctga cgcaggacga atgggcgacc ttcgaggcgc
     2341 aggccgaacg cgcgctgtcg caggggcatt tcacctcgcc gtcggaatat atcgacggca
     2401 tcaccgacct cagcgccccg atcgccagcg acgccggcgt gatcgccgcc atgaccatgc
     2461 cctacatcca cgttcacgac ggcctcaccc aggacgaggc cctcgcgaag ctcaagacca
     2521 ccgccgccac gctgagcaag gcgctgggcg gctgatcctt tccagaacaa gagtaaccca
     2581 gcgtcgcgaa ccttatggac cctttcctgc tccttcaccc ggacgacaat gtgctggtct
     2641 gccgccgtca ggccgccgcc ggtgaaaccg taaccctgga tggggacgcc gtcgccctgc
     2701 ccggcgcggt cgcggtcggg cacaaggtcg cgcgccgcgc cctgcgtcct ggcgacaagg
     2761 tggtcaagta cggcgcgccc atcggctcga tgaccgccgc cgccgcgcgc ggcgcgcacg
     2821 tccacctgca caatatgcaa agcgattaca tcccgtccca cacccgcgag gccaccggcc
     2881 atgacgactg actttccgac cttcgaggcg tggctgcggc ccgacgggcg caagggaatc
     2941 cgcaacatcg tcgtcgtcgg ctatctggtc gaatgcgcgc accatgtcgc gcgccgcatc
     3001 gtgcagacga gcggcgagac ggacgtgcat ctgatcggct ttccgggctg ctatcccaac
     3061 gcctacgcct accgcatgat ggaggcgctg tgcacgcatc cgaacgtcgg cggcgccgtg
     3121 ctggtgtccc tgggctgcga gggcttcaac cgcgagtcgc tgaagcgcca tatcgaggcg
     3181 tcgggccgcc cggtcgagac cttcgtgatc cagcaggccg gcggcacccg cgccacgatc
     3241 gaggcgggcg tcgcagccgt caaccgcatc cgcgccgagg cggccaagac tccgcgcgcg
     3301 acgatggcgg tcgccgaact ggtgatcggc acgatctgcg gcggctcgga cggcacgtcg
     3361 ggcatcaccg ccaatccggc ggtcggccgc gccttcgatg cgctgggcgc ggcgggcgcg
     3421 gcctgcatct tcgaggagac gggcgaactg gtcggctgcg agcgcatcat ggcctcgcgc
     3481 gccatcacgc cggaactggg ccttgagctt gagcgctgcg tgcagaaggc ggagcgctac
     3541 tacaccgtca tgggctacgg ctcgttcgcg cccggcaacg ccgagggcgg gctgacgacg
     3601 caggaggaaa agtcgatggg cgcctattcc aagtcgggat cggcccccat cctcggcatc
     3661 ctcaagcccg gcgatgttcc gccttcgggc gggctttacc tgctcgacgt ggtgccggac
     3721 ggcgagccgc gcttcggctt tcccaacatc tccgacaatg ccgagatcgt cgagctgatc
     3781 gcgtgcggcg cgcacctgat cctgttcacc accgggcgcg gcagcgtggt cggatcggcc
     3841 atctcgccgg tgatcaaggt gtgcgccaac ccggaaacct accgccgcct gcccgacgac
     3901 atggacgtcg acgccggacg catcatggag ggccgcgccg atatcgacac ggtcggcgac
     3961 gagatcacgg acctgaccct gcgcgtcgcg ggcggcgagg aatccgtttc cgaagcgctg
     4021 ggccacaccg aattcatcct cacctacaag agcttcgagc cggccggccc cgcctgcctg
     4081 ccgctgaagc gcgcctgacc ggagttaaga acatgggacg cctgagcggc aaaaccgccc
     4141 tgatcaccgc cgcgggccag ggcataggcc gcgccaccgc cgagatgttc gtgcgcgagg
     4201 gcgcgcgcgt catcgccacc gacatcgatc cggcggcgct cgacatcgac ggcgccgaaa
     4261 gccgaaggct cgacgtcacc gaccccgccg ccgtcaaggc catcgccgcc gaatatcccg
     4321 aagtcgacgt gctgtacaac tgcgcgggcg tcgtccatgc gggcaccatc ctcgactgcg
     4381 gcgaggacga atggaacttc gcgctgtcgc tcaacgtcac ggcgcagtac cgcatgatcc
     4441 gcgccttcct gcccgccatg atcgccaatg gcggcggcgc gatcatcaac atgtcgtcgg
     4501 tcgcctcgtc gatcaagggc gtgcccaacc gcttcgccta cggcgccacc aaggccgccg
     4561 tcatcggcct gaccaaggcc gtcgccgccg atttcgtcac caagggcatc cgctgcaacg
     4621 ccatctgccc cggcacggtc gagacgccgt ccctgc
//
LOCUS       DASVLL010000245         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_228489,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000245 DASVLL010000000
VERSION     DASVLL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228489"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF402_04035"
     CDS             <1..560
                     /locus_tag="VF402_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7799494.1"
                     /translation="SAWRMRDMVQDLLEYARMGREGLTVGEVDMQVEINHVLENLRQL
                     ILDSGAEITCDPLPKIHGSAVQIMRLFQNLIANAIKYQPEGRTPVIHIAAAEGGAYWQ
                     FSVTDNGMGIDEAFIEQIFEPFRRLHNWEAIKGTGLGLAVCKRIVENHGGRIWVESKP
                     GEGSRFHISIARALRPESAAPEKEA"
     gene            562..1029
                     /locus_tag="VF402_04040"
     CDS             562..1029
                     /locus_tag="VF402_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799495.1"
                     /translation="MVNLLIREGRPAEIMLIEDNRGDALLTARAFRDARIDNRLSVAD
                     TGEKALAMLRRQGPHAHLRLPDIILLDLNLPGMSGQDVLRAIKADAALKTIPVVVLSS
                     SEAQGDIARAYDQHANAYIVKPVSIAKFREVAASIEQFFFMLAILPAPDAHSA"
     gene            1202..>1598
                     /locus_tag="VF402_04045"
     CDS             1202..>1598
                     /locus_tag="VF402_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing esterase"
                     /protein_id="HEX7799496.1"
                     /translation="MKKALSTALLASAASMLALCAASAQAQSYSRLVSFGDSLTDNGN
                     LYTATGQPPAPYNHRFTNDRTWAEYLSGTMLGYFTPASAVNSGSIDLAWGGARADNAA
                     NSNGAIPSSYQQIQTFAAEGGQFAAGDVAS"
BASE COUNT          306 a          537 c          505 g          250 t
ORIGIN      
        1 attcggcctg gcggatgcgc gacatggtgc aggacctgct ggaatacgcg cgcatgggcc
       61 gcgaggggct gaccgtcggc gaggtcgaca tgcaggtcga gatcaatcac gtgctggaga
      121 acctgcgcca gttgatcctc gacagcggcg ccgagatcac ctgcgatccg ctgccgaaaa
      181 tccacggcag cgcggtgcag atcatgcggc tgttccagaa cctgatcgcc aacgccatca
      241 agtaccagcc cgaaggccgc acgccggtca tccacatcgc cgcggcggaa ggcggcgcct
      301 actggcagtt ctcggtcacc gacaacggca tgggcatcga cgaagccttc atcgagcaga
      361 ttttcgagcc gttccgccgc ctgcataact gggaggcgat caagggcacg ggactggggc
      421 tggcggtgtg caagcgcatc gtggagaatc acggcgggcg catctgggtc gaatcgaaac
      481 ccggcgaggg cagccgcttc cacatctcga tcgccagggc gctgcgcccg gagagcgccg
      541 cgccggaaaa ggaggcgtaa gatggtcaat ctgctgatcc gcgaaggccg ccccgccgag
      601 atcatgctga tcgaggacaa tcgcggcgac gcgctcctga ccgcgcgcgc cttccgcgac
      661 gcccgcatcg acaaccgcct gagcgtggcc gacaccggcg agaaggcgct ggccatgctg
      721 cggcggcagg ggccgcacgc gcatctgcgc ctgcccgaca tcatccttct ggacctcaac
      781 ctgcccggca tgagcggtca ggacgtgctg cgcgccatca aggccgacgc ggcgctcaag
      841 accatcccgg tggtggtgct gtcgtcgtcc gaggcgcagg gcgacatcgc ccgcgcctac
      901 gaccagcacg ccaacgccta tatcgtcaaa ccggtcagca tcgccaagtt tcgcgaagtg
      961 gccgccagca tcgaacagtt cttcttcatg ctggccatcc tgcccgcgcc ggacgcgcac
     1021 agcgcctgag gctgcggctt tcatgccaca gggtcgatga aaaagcgagg tcgcttccct
     1081 tgcggaaatc ttgtcaaacg gcggcggaag gcggatgctt tccgttgacg gaaaggtaag
     1141 ctttgcgccc gcccgtcaag ccataaaaaa gattgccata aaaattattg acagggttgt
     1201 tatgaaaaaa gctctttcca cggcgctgct ggcgtcggcc gcctccatgc tggccctttg
     1261 cgcggcgtcg gcgcaggcgc aatcctattc gcgtctggtc agcttcggcg acagcctgac
     1321 cgacaacggc aatctctata cggcgacggg acagccgccc gcgccgtaca atcaccgctt
     1381 caccaacgac cggacctggg cggaatacct gtcggggacg atgctcggct atttcacccc
     1441 ggcctcggcg gtcaattccg gcagcatcga cctcgcctgg ggcggcgcgc gcgccgacaa
     1501 cgcggccaac agcaacggcg ccatcccgtc cagctatcag cagattcaga ccttcgcggc
     1561 cgagggcggc cagttcgccg ccggcgacgt cgccagcc
//
LOCUS       DASVLL010000246         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_228500,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000246 DASVLL010000000
VERSION     DASVLL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228500"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /gene="sppA"
                     /locus_tag="VF402_04050"
     CDS             complement(<1..1695)
                     /gene="sppA"
                     /locus_tag="VF402_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX7799497.1"
                     /translation="MKSFFITFTAVVLGMVFVLVGVPLALIAIIAAASHPRVTGDVVL
                     SLDLRQKMTDQSRSSPLDIVTGGQLSTMDVVTTLHHAADDPHVKAVFVRLPEGGMSPA
                     AAEEIRNAFAVMRQANKPVIAHSQGLYPDTMVAASYMLGASASELWMQPRASFQVTGV
                     ATSTMFLKRAFDKYGVKAEYEQRAEFKNAVNPYLYDDYTPAHREATLGWMNGIYSDML
                     SDIAHDRRMDPVKLRATLEAGPYGAEQAAQLKLVDRLGQVQEAEDAALKRGDDAKLMD
                     IGDYEKTIRPANTGATIAVIDGEGAIMTGKGGGGGFNRDPQMMSDDVADAFYNAARDK
                     AVKAIVFRVSSPGGSDTASEQIAEAMKAAKAAGKPVVVSMGEYAASGGYWISAGANAI
                     VANPSTLTGSIGVFGGKIAYGEALSRFGVDLRDISVGSPYSGAYMPTQGFTPAQRAAL
                     SGWIDQIYAGFISHVAQGRNLPEATVRDIARGRVWTGGQALGLHLVDKTGGFYDAVDT
                     AKALAHIDKGAAVRLVDYDTQTGLFGKARQNVSLSLSGLKALSFLGWAMSDPKAQAL"
     gene            complement(1769..2644)
                     /locus_tag="VF402_04055"
     CDS             complement(1769..2644)
                     /locus_tag="VF402_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEX7799498.1"
                     /translation="MSQPRFVIAAFYHFFDFPQFEAMQAPLLARLQALGIKGSLLITR
                     EGVNSTISGAREAIDDFLRYLQDEVVGGPIRWKESHADFQPFGKARVRLKKETISLGE
                     PVSMRAFGQYVAPKDWNDLIRRDDVVLIDTRNDYEVNLGAFAGAVDPVIPNFKHLPQW
                     TRDNAGALQGKKIATYCTGGIRCEKYTSWLLDQGFEDVYHLQGGILQYLEDVPQDRSL
                     WRGECFVFDERIAVDHRLQPSATAVLCLHCDHALTAEDQAHPAYVKGESCPHCLGAPL
                     HAHDQPKRHPGRVKF"
     gene            complement(2664..3137)
                     /locus_tag="VF402_04060"
     CDS             complement(2664..3137)
                     /locus_tag="VF402_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HEX7799499.1"
                     /translation="MKAHLAPGAVAYNRLMADSFQRFTGLPILTGADALSDVALAEAM
                     FAADAAIVSHGVEADPVFRYANARALALWEMDWAAFTRLPSRLSAEPAGEIQADRDAL
                     LREALTKGFVDRYEGVRVSRTGRRFRIENTVLWNVVDAAGRRYGQAAFIRNWRHL"
BASE COUNT          554 a         1071 c         1104 g          549 t
ORIGIN      
        1 cagcgcctgc gccttgggat cgctcatcgc ccagccgagg aaggacaggg ccttgaggcc
       61 gctcaggctc aggctgacat tctggcgcgc cttgccgaac aggccggtct gcgtgtcgta
      121 gtccaccagc cggacggcgg cgcccttgtc gatgtgcgcc agcgctttgg ccgtgtccac
      181 cgcgtcgtag aagccgccgg tcttgtcgac cagatgcagg cccagcgcct gaccgccggt
      241 ccagacgcgg cccctggcga tatcgcgcac cgtggcttcg ggcaggttgc gcccttgggc
      301 gacgtggctg atgaagcccg cgtaaatctg gtcgatccag ccggagaggg cggcgcgctg
      361 cgcgggcgtg aagccctgag tcggcatgta ggcgccgctg taggggctgc cgaccgagat
      421 gtcgcgcagg tcgacgccga agcggctcag cgcctcgcca taggcgatct tgccgccgaa
      481 cacgccgatc gagccggtca gggtgctggg gttggcgacg atggcgttgg cgcccgccga
      541 gatccagtaa ccgcccgaag ccgcgtattc gcccatcgac accacgaccg gcttgcccgc
      601 cgcctttgcc gccttcatgg cttcggcgat ctgttcggac gccgtgtccg agccgccggg
      661 cgaggagacg cggaacacga tggccttgac cgccttgtcc ctggccgcgt tgtagaaggc
      721 gtcggccacg tcgtcggaca tcatctgcgg gtcgcggttg aagccgccgc cgccgccctt
      781 gccggtcatg atcgcgcctt cgccgtcgat gacggcgatg gtcgcgcccg tattcgcggg
      841 gcggatggtc ttttcatagt cgccgatgtc catcagcttg gcgtcgtcgc cgcgtttcag
      901 ggcggcgtcc tcggcctcct gcacctgccc cagacggtcg accagcttga gctgcgcggc
      961 ctgttcggcg ccgtagggac cggcctccag cgtcgcgcgc agcttgaccg ggtccatgcg
     1021 gcggtcatgc gcgatgtcgc tcagcatgtc gctgtaaata ccgttcatcc agcccagggt
     1081 cgcctcgcgg tgcgcgggcg tgtaatcgtc gtaaaggtac ggattgacgg cgttcttgaa
     1141 ctcggcgcgc tgctcgtatt cggccttgac gccgtacttg tcgaaggcgc gcttcaagaa
     1201 catggtcgag gtcgcgacgc cggtcacctg aaacgaggcg cgcggctgca tccacaattc
     1261 gctggccgag gcgcccagca tgtaggaggc ggcgaccatg gtgtcgggat aaaggccctg
     1321 actgtgggcg atcaccggct tgttggcctg acgcatgacg gcgaaggcgt tgcggatctc
     1381 ctcggcggcg gcgggcgaca tgccgccttc gggcaggcgc acgaacaccg ccttgacgtg
     1441 cgggtcgtcg gcggcgtggt gcagggtcgt caccacgtcc atggtcgaca actgcccgcc
     1501 ggtcacgatg tccagcggac tggagcggct ctggtccgtc atcttctggc gcaggtccag
     1561 cgacagcacg acgtcgcccg tgaccctggg gtgcgaagcg gcggcgatga tcgcgatcag
     1621 cgcgagcgga acgccgacca acacgaaaac catgccgagc acgacggcgg tgaaggtgat
     1681 gaaaaacgat ttcatgaaaa acccggatgc cccctcggct gcgggctcag cataaccgca
     1741 aaagccataa ggtcaaatga acagcttgtc agaatttgac gcggcccgga tggcgcttcg
     1801 gctggtcgtg cgcgtgcagc ggcgcgccga ggcagtgcgg gcacgattcg cccttcacat
     1861 aggccggatg cgcctgatcc tcggcggtca gggcgtggtc gcaatgcagg cacagaaccg
     1921 ccgtcgccga gggttgcagc cggtgatcga ccgcgatgcg ctcgtcgaac acgaagcatt
     1981 cgccgcgcca cagcgaccgg tcctgcggca cgtcctccag atattgcagg atgccgccct
     2041 gaaggtgata gacgtcctcg aacccctgat cgagcagcca cgaggtgtac ttctcgcagc
     2101 ggatgccgcc ggtgcagtag gtggcgatct tcttgccttg caacgccccg gcgttgtcgc
     2161 gcgtccattg cggcagatgc ttgaagttcg gaatgacggg atcgacggcg cccgcgaagg
     2221 cgcccagatt gacctcgtaa tcgttgcgcg tgtcgatcag cacaacgtcg tcgcgccgga
     2281 tcaggtcgtt ccagtccttc ggcgcgacat attgcccgaa agcgcgcatc gacaccggct
     2341 cgcccagcga gatcgtctcc ttcttcagcc gcacgcgcgc cttgccgaac ggctggaagt
     2401 cggcatggga ctccttccac cggatcggcc cgccgaccac ctcgtcctga agatagcgca
     2461 ggaagtcgtc gatggcctcg cgtgcgccgg aaatcgtcga attgacgcct tcgcgcgtga
     2521 tcagcagcga gcccttgatg ccgagcgcct gcaaccgcgc cagcagcggc gcctgcatgg
     2581 cctcgaactg cgggaaatcg aagaaatgat agaaggcggc gatgacgaaa cgaggctggg
     2641 acatgccgcc gcctagcata aagtcacaga tggcgccagt tgcggatgaa ggcggcctga
     2701 ccgtaacggc ggcccgccgc gtcgaccacg ttccacagga cggtgttttc gatgcgaaag
     2761 cgccgtcctg tgcgggagac gcgcacgcct tcgtaacggt cgacaaagcc cttggttagc
     2821 gcctcgcgca gcagggcgtc gcggtcggcc tgaatctctc cggcgggttc ggcgctcagg
     2881 cgcgagggca ggcgggtgaa ggcggcccag tccatctccc acagcgccag cgcccgcgca
     2941 ttggcgtagc ggaaaaccgg atcggcctcg acgccgtgcg acacgatggc cgcgtccgcc
     3001 gcgaacatcg cctcggccag cgccacgtcg ctcagcgcgt ccgcgcccgt aaggatggga
     3061 aggccggtga aacgctgaaa actgtccgcc atcagacggt tataggcgac ggcgccgggc
     3121 gcgagatggg ctttcatgcc gccactcaaa ccgaccggcg ccgcaaaagc aagcgcgcgc
     3181 cgatctgttc acaggataaa gccgtttccc tgtccgcgcg ccggaaaaaa gttatggcgg
     3241 gggattgcgc tggcggaaac cttgcgctat ttccacgg
//
LOCUS       DASVLL010000247         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_228515,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000247 DASVLL010000000
VERSION     DASVLL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228515"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="ftsY"
                     /locus_tag="VF402_04065"
     CDS             <1..888
                     /gene="ftsY"
                     /locus_tag="VF402_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7799500.1"
                     /translation="TLTESVTHVFAQKEALDAAALEDLEDLLIESDLGPAVAATIAEK
                     MAKARFSSAKDSGAVRAVLAEALAEELAGHEATFDPLSGPKPYVVLFVGVNGSGKTTT
                     LGKIAASLTARGARVLVVAGDTFRAAAVEQLKVWAERAKADFMGRKTGADAAGLAYDA
                     FVRAKEEGYDVVLIDTAGRLQNKQQLMDELLKVVRVIKKVDPEAPHETLLVLDATVGR
                     NALSQEQIFGRQAFVSGLVMTKLDGTARGGILIPVAKASDAPIKLIGVGEDIEDLHAF
                     RARDFARSMVGLEPLGDAQ"
     gene            885..1601
                     /locus_tag="VF402_04070"
     CDS             885..1601
                     /locus_tag="VF402_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEX7799501.1"
                     /translation="MSAGNLKDEIENGDEIVDRAGAQAAEKLGEINPELALQPGQPKQ
                     NHVKMAVDYGPLIAFGLTFLVCRFFKMTDKSEPLIIASGVLGAASLIAFIGGLALEKR
                     IAWIPLLSALIAVPFSLLTVFFHDTVFVKIKMTIIDALIGAILIGGLMLRKQPLKALL
                     GEALRLKETAWPRLTLYYALFYFAMAAINEVVWRTQSDDVWVTWKMISIIGGPIAFSI
                     ALLPFLMKNMILEPDTDGQP"
     gene            1696..>2242
                     /locus_tag="VF402_04075"
     CDS             1696..>2242
                     /locus_tag="VF402_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7799502.1"
                     /translation="MHDTSSAARHALDDSPSHLLHRVLQIALDIYGAELGEGALSQRQ
                     YAVLSALDGREGLTQADLVKTTGIDRSTLADLVARLAGKGLLARERSSSDGRANRVQL
                     TEAGRAALAEAAPKVAAADAKILELLSPPKRDSFLKLLRKLVHARDGEPDRPEKAHKK
                     ADKSAKKKKSGGDKPRKKDKLA"
BASE COUNT          413 a          709 c          758 g          362 t
ORIGIN      
        1 accctgaccg agtccgtcac gcatgtgttc gcgcagaagg aggcgctgga cgccgccgcg
       61 ctggaagacc tcgaagacct gctgatcgag agcgatctcg gccccgccgt cgccgccacg
      121 atcgccgaaa agatggcgaa agcgcgcttt tcgtccgcca aggattccgg cgccgtgcgc
      181 gccgtactgg ccgaagcgct ggccgaggag ctggcgggcc acgaagcgac gttcgatccc
      241 ctgtcaggcc cgaagcccta tgtcgtgctg ttcgtcggcg tcaacggctc cggcaagacg
      301 acgacgctgg gcaagatcgc cgcctcgctg accgccaggg gcgccagggt gctggtggtg
      361 gcgggcgata ctttccgcgc cgccgccgtc gaacagctca aggtctgggc cgagcgcgca
      421 aaagccgact tcatgggccg caagaccggc gccgacgcgg cgggcctcgc ctatgacgcc
      481 tttgtgcgcg cgaaggaaga gggctatgac gtcgtgctga tcgacacggc gggccggttg
      541 cagaacaagc agcaactgat ggacgagctt ttgaaggtgg tgcgcgtgat caagaaggtc
      601 gacccggagg cgccgcacga gacgctgctg gtgctggacg ccacggtcgg gcgcaacgcc
      661 ctgtcgcagg agcagatttt cgggcgtcag gccttcgtct ccggcctcgt catgaccaag
      721 ctggacggca cggcgcgcgg cggcatcctg atcccggtcg ccaaggcgtc cgacgcgccg
      781 atcaagctga tcggggtcgg cgaggacatt gaggatctgc acgccttcag ggcgcgcgac
      841 ttcgcccgct cgatggtcgg gctggaaccg ctgggggatg cgcaatgagc gccggaaacc
      901 tgaaggacga gatcgaaaac ggcgacgaaa tcgtcgaccg cgccggggcg caggccgccg
      961 aaaagctcgg cgaaatcaat cccgaactgg cgctgcaacc gggccagccg aagcagaacc
     1021 acgtcaagat ggccgtcgat tacgggccgc tgatcgcgtt cgggctgacc ttcctcgtct
     1081 gccgtttctt caagatgacc gacaagagcg agcctttgat catcgcgtcg ggcgtgctgg
     1141 gcgccgcctc gctgatcgcc tttatcggcg ggctggcgct ggaaaagcgc atcgcgtgga
     1201 ttccgctgct gtcggcgctg atcgccgtgc cgttctcgct gctcaccgtc ttcttccacg
     1261 acaccgtgtt cgtgaagatc aagatgacca ttatcgacgc cctgatcggc gcgatcctga
     1321 tcggcggcct catgctgagg aaacagccgc tcaaggcgct gctgggcgag gcgttgaggc
     1381 tgaaggagac ggcgtggccg cgcctgaccc tctattacgc cctgttctat ttcgcgatgg
     1441 cggcgatcaa cgaagtcgtc tggcgcacgc aaagcgacga cgtctgggtg acgtggaaga
     1501 tgatctcgat catcggcggg ccgatcgcct tttccatcgc gctgctgccg ttcctgatga
     1561 aaaacatgat ccttgagccg gacacggacg gccagccgta attttttctt ccgtgtgcgg
     1621 agcaaactgt cgtaaattcg tcatcggaat gtggcaatct cggctgccat gaaagattcg
     1681 agacgcggta aagccatgca cgatacctcc tcagccgcgc gccacgcgct cgacgactcg
     1741 ccgtcgcatc tgctgcaccg ggtgttgcag atcgcgctcg acatctacgg cgcggagctg
     1801 ggcgagggcg cgctgtcgca gcgccagtac gcggtgctga gcgcgctgga cggccgcgag
     1861 gggctgacgc aggcggatct ggtcaagacc accggcatcg accgctccac gctcgccgat
     1921 ctggtcgcgc gactggcggg caaggggctt ctggcgcgcg aacgctcgtc cagcgacggg
     1981 cgcgccaatc gggtgcaact gaccgaggcg ggccgcgccg ccttggccga ggccgcgccg
     2041 aaggtcgccg ccgccgatgc gaagattctc gaactgttgt cgccgccgaa gcgcgacagc
     2101 ttcctgaaac tgctgcgcaa gctggtccat gcccgcgacg gcgaacccga ccggccggaa
     2161 aaggcgcata agaaggcgga taagtcggcc aaaaagaaaa agtccggcgg ggataagccg
     2221 cgcaagaagg acaagctggc ca
//
LOCUS       DASVLL010000248         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_176100,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000248 DASVLL010000000
VERSION     DASVLL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176100"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..805
                     /locus_tag="VF402_04080"
     CDS             104..805
                     /locus_tag="VF402_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7799503.1"
                     /translation="MRVLLIEDDSATAQSIELMLKSEGFNVYTTDLGEEGVDLGKIYD
                     YDLILLDLSLPDMSGLEVLRQLRVGKVNTPVMILSGTSEIDTKVKTLGGGADDYMTKP
                     FHKDELIARIHAVVRRSKGHAQSVIRTGDIVVNLDAKTVEVAGNRVHLTGKEYEMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAVAAGGNHYIETVWGRG
                     YVLRDPHDQGSIVAA"
     gene            984..1322
                     /locus_tag="VF402_04085"
     CDS             984..1322
                     /locus_tag="VF402_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7799504.1"
                     /translation="MTNDDAVVNLFALAHPGRLNVFRTIAQAGPDGMAAGKIAKSVGI
                     PANTASTHLSILAGAGLLNAYRDGRSIIYRADFDHYADLMRFLLVDCTENHPEAALLR
                     AGMAPLEKRG"
     gene            1319..1669
                     /gene="arsC"
                     /locus_tag="VF402_04090"
     CDS             1319..1669
                     /gene="arsC"
                     /locus_tag="VF402_04090"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521411.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX7799505.1"
                     /translation="MSAYVIYHNPNCSTSRKALEILRGAGLDPQVVEYLKTGWTKAQL
                     QGLFAAMGKTPRDLLRVRGTPAEELGLTAPDASDDAILDAMVRHPVLVERPIVVTPKG
                     TILARPQDKLNEVL"
     gene            1852..2424
                     /locus_tag="VF402_04095"
     CDS             1852..2424
                     /locus_tag="VF402_04095"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX7799506.1"
                     /translation="MTFKAAYKVTAVLAALATFGALNAQAQTAPETAPPAAAAPAQAA
                     PADASNPAKDFIRQAFLANEFGIAAAQVAIAKAQSPATKAAAQSVLNDGMKVRQDMIT
                     AIQGSTSDMHFDQSWDADYKARLADLQTAADADFDQKFAATEGQILTQAASAASAYAA
                     TGGDAAVKTFAAGAQPIFQADAAKLAATAG"
     gene            complement(2490..>2907)
                     /locus_tag="VF402_04100"
     CDS             complement(2490..>2907)
                     /locus_tag="VF402_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7799507.1"
                     /translation="PTLEELGIGFVPYSPLGRGFLTGALQDAPLDPSDYRNTSPRFVG
                     EAAKTNARAVDALKDLAAAKGVTPAQLALAWLLAQKPWIVPIPGTRRLERLKENIASA
                     DIDLTRADLDAIEDAIARTGFAGARYTEGGMAMLNR"
BASE COUNT          558 a          917 c          909 g          523 t
ORIGIN      
        1 taagcttaac gaatcttaag tgatttcctg tttagcatga aggttaacgg gcgaaagcgt
       61 gttgccttcg ctatcctgtt acctcaagcc gggaggggct tacatgcgcg tattgctgat
      121 tgaagacgac agcgccaccg ctcagagcat cgaattgatg ctcaaatcgg aaggttttaa
      181 cgtctatacg accgatctgg gcgaggaagg cgtcgatctg ggcaagatct acgactacga
      241 cctgatcctg ctcgatctgt cgctgccgga catgtcgggc ctcgaagtgc tgcgccagtt
      301 gcgcgtcggc aaggtcaaca ccccggtcat gatcctgtcg ggcacgtcgg aaatcgacac
      361 caaggtcaag accctgggcg gcggcgccga cgactatatg accaagccct tccacaagga
      421 cgaactgatc gcgcgtattc acgccgtggt ccgccgctcc aagggccatg cgcaatcggt
      481 catccgcacc ggcgacatcg tcgtcaatct cgacgccaag acggtcgagg tcgcgggcaa
      541 ccgcgtgcac ctgaccggca aggaatacga aatgctggag ctgctttccc tgcgcaaggg
      601 cacgacgctc accaaggaaa tgttcctgaa ccacctgtac ggcggcatgg acgaacccga
      661 actgaagatc atcgacgtgt tcatctgcaa gctgcgcaag aagctggcgg tggccgcggg
      721 cggcaaccac tatatcgaaa ccgtttgggg ccggggctat gtgctgcgcg acccgcacga
      781 tcagggttcg atcgtcgcgg cgtaagcgcc ggatcggaat ttccggaaat tttgaatgaa
      841 gcccgccgcc ctcccgcggc gggctttgtc gtgcgcgggg ccgctcgcgg aaattttatg
      901 gcggcgcaac aggcgcgcgc ctttatgttt ccatagtttt gcgataacca gcctgcgatg
      961 ttttcagctt ttcgggatgt ccgatgacga acgacgacgc ggtggtgaac ctgtttgcgc
     1021 ttgcgcaccc cggaaggctc aatgtgttcc gcacgatcgc gcaggccggt cccgacggca
     1081 tggcggcggg caagatcgcc aaaagcgtcg gtattccggc caacaccgcc tcgacgcatc
     1141 tgagcattct ggccggggcc gggctgttga acgcatatcg cgacgggcgc tcgatcatct
     1201 atcgcgccga tttcgaccac tatgccgacc tgatgcgctt cctgctggtc gactgcaccg
     1261 aaaaccatcc tgaggcggcg ctgctgcgcg ccgggatggc gccgctggag aagcgggggt
     1321 gagcgcttac gtcatctatc acaatcccaa ctgctcgacc tcgcgcaagg cgctggagat
     1381 tttgcgcggc gcgggcctcg acccgcaggt ggtcgagtat ctgaaaaccg gctggaccaa
     1441 agcgcagttg caggggctgt tcgcggccat gggcaagacg ccgcgcgacc tgttgcgcgt
     1501 gcgcggcacg cccgccgagg agttgggcct gaccgcgccg gacgcaagcg acgacgcgat
     1561 tctggacgcg atggtccggc atccggttct ggtcgagcgc ccgatcgtcg tgacgccgaa
     1621 gggcacgatc ctggcgcgtc cgcaagacaa gctgaacgaa gttctttaat ttataagtct
     1681 attttaaaaa cgtccaagct taaccgcctt gacgccctct ttataaaagc acggcgaggc
     1741 ccaagggcga tatttatgaa tccggcccaa ttttgaacat ctgcgccttt aatgtgacag
     1801 gctccgccgt ttccttgcgg gatggctcac aatattaaag ggatagatgt tatgacattc
     1861 aaagccgctt acaaggtcac cgctgtgctg gccgcgcttg cgactttcgg cgcgctgaac
     1921 gcgcaggccc agactgcgcc ggagaccgcg ccgcccgctg cggccgctcc agcgcaggcc
     1981 gcgcccgccg atgcgtccaa cccggccaag gatttcatcc gtcaggcctt cctcgccaac
     2041 gaattcggca tcgccgccgc gcaggtcgcc atcgccaagg cgcagtcgcc ggccaccaag
     2101 gccgccgcgc aaagcgtgct caatgacggc atgaaggtgc gtcaggacat gatcaccgcc
     2161 attcagggct cgacctcgga catgcatttc gaccagagct gggacgccga ttacaaggcc
     2221 aggctggccg atctgcaaac ggcggcggac gccgatttcg accagaaatt cgccgccacc
     2281 gaaggccaga tcctgacgca ggccgcgtcg gcggccagcg cctacgccgc cacgggcggc
     2341 gacgccgcgg tcaagacgtt cgcggcgggc gcgcagccaa tctttcaggc cgacgcggcc
     2401 aagctggcgg cgacggccgg ttagaaaccc ggttagaaac ccatttagaa acgatgaggc
     2461 cccggcggac gaccggggcc ttttcatgtt taccggttga gcatagccat gccgccctcg
     2521 gtgtagcgcg cgcccgcgaa accggtcctg gcgatcgcgt cctcgatggc gtccagatcg
     2581 gcgcgcgtca ggtcgatgtc ggcgctggcg atattctcct tcagccgctc cagacggcgc
     2641 gtgccgggaa tcgggacgat ccacggcttt tgcgccagca gccaggccag cgccagttgc
     2701 gcgggcgtca cgcccttggc ggcggccaga tccttcagcg cgtcgaccgc gcgggcgttg
     2761 gttttcgccg cctcgccgac gaagcgcggg ctggtgttgc gatagtccga cggatcgagc
     2821 ggcgcgtctt gcagcgcgcc ggtcaggaag ccgcgcccca atggcgaata gggcacgaaa
     2881 ccgatgccca gctcctccag agtcggc
//
LOCUS       DASVLL010000249         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_14484, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000249 DASVLL010000000
VERSION     DASVLL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14484"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /gene="cysD"
                     /locus_tag="VF402_04105"
     CDS             <1..538
                     /gene="cysD"
                     /locus_tag="VF402_04105"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX7799508.1"
                     /translation="AHGFDAAFGGARRDEEKSRAKERIFSFRTANHGWDPKNQRPELW
                     NLYNARVKPGESIRVFPLSNWTELDIWQYILMENIPIVPLYFAAKRPVVERGGQWIMV
                     DDERLPLNPGETPQMKSVRFRTLGCYPLTAAVESEAATLEEIVAEMLVARTSERSGRL
                     IDHDEQGSMEKKKREGYF"
     gene            540..>2161
                     /gene="cysN"
                     /locus_tag="VF402_04110"
     CDS             540..>2161
                     /gene="cysN"
                     /locus_tag="VF402_04110"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX7799509.1"
                     /translation="MNAIVETKLDTQALVDYLAAHEQKDLLRFLTCGSVDDGKSTLIG
                     RLLYDTKLIFEDQLAGIEKDSKKFGTTGDDIDLALLVDGLQAEREQGITIDVAYRFFT
                     TDKRKFIVADTPGHEQYTRNMATGASNSDLAVILIDARKGILTQTRRHSFIVSLLGIK
                     HVVLAINKIDLMDYSEGVFEQILADYQAFAAEFGFKTLQAIPMSARYGDNVLSRSDNM
                     PWYKGPTLVEHLETVDVSDDRSGKPFRLQVQWVNRPNLDFRGFSGTIASGTVRPGDPV
                     IVAGSGKASTVKSIVTYDGELPVAGPQQAVTLTLSDEIDISRGDVICPPDARPQVADQ
                     FQARLIWMSEQELIPGRQLLIKTGARTLAASVTEIRHKTDVNTFAPLAAKTLHLNEVA
                     VVNIATAEPIAFDAYNDNHAMGAFIVIDRVSNLTLGAGMIEHGLRRATNVHWQALDVS
                     KATRAAAKGQKPAVLWFTGLSGAGKSTIANLVEKKLLSLGHHTYLLDGDNVRHGLNKD
                     LGFTDADRVENIRRVAEVAKLMVDAGLITLVS"
BASE COUNT          430 a          753 c          637 g          341 t
ORIGIN      
        1 cgcgcacggc ttcgacgcgg ccttcggcgg cgcccggcgc gatgaggaaa agtcccgcgc
       61 caaggagcgc atcttctcgt tccgcaccgc caatcacggc tgggatccga agaaccagcg
      121 cccggaattg tggaacctct acaacgcccg cgtcaagccg ggcgaatcga tccgcgtctt
      181 cccgctctcg aactggaccg aactcgacat ctggcaatat atcctgatgg agaacatccc
      241 catcgtgccg ctgtacttcg ccgccaagcg cccggtcgtg gagcgcggcg gccagtggat
      301 catggtcgat gacgaacgcc tgccgcttaa tcccggcgag acgccgcaga tgaagtcggt
      361 gcgcttccgc acgctgggct gctatccgct gaccgccgcc gtcgaatccg aggccgccac
      421 gctggaggag atcgtcgccg aaatgctggt ggcgcgcacg tctgagcgct caggccgcct
      481 gatcgaccac gacgagcagg gctcgatgga aaagaagaag cgcgaagggt atttttaaga
      541 tgaacgcgat tgtcgaaacc aagctcgata cgcaggcgct cgtcgattac ctggccgcgc
      601 acgaacagaa ggatctgctg cgcttcctga cctgcggcag cgtcgatgac ggcaagtcga
      661 ccctgatcgg gcgcctgctc tacgacacca agctgatttt cgaggatcaa ctggcgggca
      721 tcgagaagga ctcgaaaaag ttcggcacca cgggcgacga catcgacctg gccctgctgg
      781 tcgacggctt gcaggccgag cgcgagcaag gcatcaccat cgacgtcgcc taccgtttct
      841 tcaccacgga caagcgcaag ttcatcgtcg ccgacacgcc cggccacgag caatataccc
      901 gcaacatggc gaccggcgcg tcgaattccg acctcgccgt catcctgatc gacgcgcgca
      961 agggcatatt gacccagacg cgccgccact cgttcatcgt gtcgctgctg ggcatcaagc
     1021 acgtcgtgct ggcgatcaac aagatcgacc tgatggacta ttccgaaggc gtgttcgagc
     1081 agatccttgc cgattatcag gcgttcgccg ccgagttcgg cttcaagacc ctgcaagcga
     1141 tcccgatgtc ggcgcgctat ggcgacaacg tgctgagccg cagcgacaac atgccgtggt
     1201 acaagggccc caccctggtc gagcatctcg aaaccgtcga cgtctccgac gaccggagcg
     1261 gcaaaccgtt ccggcttcag gtccagtggg tcaaccgccc gaacctcgac ttccgcggct
     1321 tctccggcac gatcgcgtcg ggcacggtcc ggccgggcga tcccgtgatc gtcgccggct
     1381 ccggcaaggc gtccacggtc aaatccatcg tcacctatga cggcgaattg cccgtcgccg
     1441 ggccgcagca ggccgtgacc ctgaccctga gcgacgagat cgacatctcg cgcggcgacg
     1501 tgatatgccc gcccgacgcg cgcccgcagg tcgccgacca gttccaggcc cggctgatct
     1561 ggatgagcga gcaggaactg atccccggac ggcaactgct gatcaagacc ggcgcgcgca
     1621 ccctcgccgc ctcggtgacc gagatcaggc acaagacgga cgtcaacacg ttcgcgccgc
     1681 tggcggccaa gacgctgcac ctgaacgagg tcgcggtcgt caacatcgcg accgccgaac
     1741 ccatcgcctt cgacgcctat aacgacaatc acgccatggg cgccttcatc gtcatcgacc
     1801 gcgtgtcgaa cctgactctg ggcgccggca tgatcgaaca cggcttgcgc cgcgccacca
     1861 acgtccactg gcaggcgctc gacgtctcca aggcgacccg cgccgccgcc aagggccaga
     1921 aacccgccgt cctgtggttc accggccttt ccggcgcagg caagtcgacc atcgccaacc
     1981 tggtggaaaa gaagctcctg agcctcggcc accacaccta cctgctggac ggcgacaatg
     2041 tccgccacgg cctgaataaa gacttaggct tcaccgacgc cgaccgcgtc gaaaacatcc
     2101 gccgcgtcgc cgaagtcgcc aaactgatgg tcgacgcggg cctgatcacc ctcgtctcct
     2161 t
//
LOCUS       DASVLL010000250         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_283857,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000250 DASVLL010000000
VERSION     DASVLL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283857"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VF402_04115"
     CDS             <1..854
                     /locus_tag="VF402_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEX7799510.1"
                     /translation="SAGHRAATTVAQTTLSSFRPQAVQPLLNLNRPSPAQIATVRPYL
                     APDPAPDPAPFAPTARVEAAAGYPAPFYGVAEAEPETAAPEPAPADAFPLGAARAQLH
                     ETYIIAQTADGLVIVDQHAAHERLVYEEMKAAMAAGHVQSQALLIPDIVDLERDEAAR
                     LMARADDLRDMGLTLEAFGPSSVLVREIPALLGGGDIAGLVRDLADDLSEHGELLALK
                     ERMAEICGDHACRHSVRSGRRLNADEMNALLRQMEATPHSGQCNHGRPTYVELKLKDI
                     ERLFGRR"
     gene            complement(901..1053)
                     /locus_tag="VF402_04120"
     CDS             complement(901..1053)
                     /locus_tag="VF402_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799511.1"
                     /translation="MDFQTIDATRTRAPKRGADALKLLLLTGLSVQAWGMLMGVSLIV
                     LNLTHR"
     gene            complement(1294..>1746)
                     /locus_tag="VF402_04125"
     CDS             complement(1294..>1746)
                     /locus_tag="VF402_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX7799512.1"
                     /translation="EWAPAFSGARVMDVFAGSGALGFEAMSRGAAFCLFVETDEAARG
                     AIRDNVESFGLFGTTRVHRRDAAQLGQRPGGPSEAFDLVFLDPPYRKGLGEKALDALL
                     KGGWLAPDAVIVFEYASDEDIFITDMWQIINTKTYGAAQVSFLIQKNS"
BASE COUNT          344 a          584 c          526 g          292 t
ORIGIN      
        1 cctcggcggg gcatcgcgcc gcgaccaccg tggcgcagac caccctgtcc agcttccgcc
       61 cgcaggcggt gcagccgctt ctcaacctca accgcccgtc gcccgcgcag atcgcgacgg
      121 tgcggcctta cctcgcgccc gatccagcgc ccgatccggc gcccttcgcg ccgaccgcgc
      181 gcgtcgaggc cgccgccggc tatccggcgc ctttttacgg cgtggccgag gccgagccgg
      241 aaaccgccgc gcccgaaccg gcgcccgcgg acgccttccc gctgggcgcc gcgcgcgcgc
      301 aactgcatga gacctacatc atcgcccaga ccgccgacgg gctggtcatc gtcgatcagc
      361 acgccgcgca tgagcggctg gtgtacgagg agatgaaggc ggcgatggcg gcgggccatg
      421 tgcagagcca ggccctgctg atcccggaca tcgtcgatct ggagcgcgac gaggcggcgc
      481 ggctgatggc gcgcgccgac gatttgcgcg acatggggct gactctggaa gccttcggac
      541 cgtccagcgt gctggtgcgc gagattcccg ccctgctcgg cggcggcgac atcgccggtc
      601 tggtgcgcga tctggccgac gacctgagcg agcacggcga attgctggcg ctgaaggagc
      661 gcatggcgga aatctgcggc gaccacgcct gccgccacag cgtgcggtcg ggccgccgcc
      721 tcaacgccga cgagatgaac gccctgctgc gccagatgga ggccacgccc cattccggcc
      781 agtgcaatca cggccgcccc acctatgtcg agttgaagct caaggacata gaaagattgt
      841 tcgggcggcg ctgacgccaa aaaaaatccc gcgccccgaa ggcgcaggat cggctcgaaa
      901 tcagcgatgc gtcagattga gaacgatgag gctgaccccc atcaacatgc cccaggcctg
      961 tacgctcagc ccggtcagca gcagcagttt cagcgcatcg gcgccgcgct tcggcgcgcg
     1021 cgttctggtc gcgtctatag tttggaaatc catcacgtca cccctatgtt ttgcagtctt
     1081 tccagcggaa ggcgacggtc cggcccctcc cccgttacaa aacgcgcagc aatttttgtg
     1141 ccttatcgat ggcggccgct gtttacgcct tgttaaggcc atactgtgga caagatcgga
     1201 aaaccgtcaa tatataattt taacaagtgc gccaattatg ccacttatgc gataaggaaa
     1261 taataaaaat ataataaaat caatgaaata gaattatgaa tttttttgta tcaagaaaga
     1321 aacctgcgcc gcgccatagg ttttcgtgtt gataatttgc cacatatccg tgataaaaat
     1381 atcttcatcg cttgcatatt cgaaaacgat cacggcgtcc ggggccagcc agccgccctt
     1441 gagcagcgcg tccagcgcct tttcgccaag ccccttacga tagggcggat cgagaaagac
     1501 cagatcgaag gcttcggacg gaccgccggg gcgttgcccc agttgcgcgg cgtcgcggcg
     1561 atgcacgcgc gtcgtcccga aaagaccgaa gctttcgaca ttgtcccgaa ttgcgccgcg
     1621 cgccgcctcg tccgtctcga cgaacaggca gaaggcggcg ccgcgcgaca tcgcctcgaa
     1681 gccgagcgcg ccggagccgg cgaacacgtc catcacgcgc gcgcccgaaa acgccggcgc
     1741 ccattc
//
LOCUS       DASVLL010000251         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_336972,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000251 DASVLL010000000
VERSION     DASVLL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336972"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VF402_04130"
     CDS             complement(<1..937)
                     /locus_tag="VF402_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7799513.1"
                     /translation="MARYNPKEAEPRQLARWAAANTFLTKADPQKPKYYVLEMFPYPS
                     GRIHMGHVRNYAMGDLVARFKRARGFNVLHPMGWDAFGMPAENAAMERRIHPKGWTYA
                     NIANMRGELQRLGLSIDWTREFATCDPAYYGQQQKWFLDLLKHDLVYRRESQVNWDPV
                     DMTVLANEQVIDGRGWRSGAVVEKKKLTQWFMRITRFADDLLDGLTTLERWPDKVRAM
                     QVNWIGKSRGLKMRWAFDGAAPEGFADGIEVYTTRPDTLFGAAFIAIAADHPLARAAA
                     ERDPALAAFIASLNKGGVSQADIDTAEKQGFDTGLR"
     gene            complement(970..1494)
                     /locus_tag="VF402_04135"
     CDS             complement(970..1494)
                     /locus_tag="VF402_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEX7799514.1"
                     /translation="MTMMRKTALATVALGVCLSFTACASLGGKPGGPSAPASANTTEP
                     ETGTPLSHLFGGGHAKKNDYNAQAGIGVNAYLWRASLDTISFMPLASADPWGGTIITD
                     WYANPQKPDERFKITVYILDSRLRADALSVSVYKQVQQNGQWTNADVSQQTQVDIENA
                     ILSKARELRLSDVK"
     gene            1942..2400
                     /locus_tag="VF402_04140"
     CDS             1942..2400
                     /locus_tag="VF402_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799515.1"
                     /translation="MKPSVSLLLSATVLALAGTSGHALAVTPVQKTVHAQQGAATGGG
                     LNLAPISQDSYISDSTTSFSKNPSKTYQFDLSNKWGVRFDVNQTETSPSGLNDIDAGA
                     FYKLTPSLRVGGSLGFGEKTDPNKTEAANAAVAQDARKQPRVRLETTFKF"
     gene            complement(2397..3047)
                     /locus_tag="VF402_04145"
     CDS             complement(2397..3047)
                     /locus_tag="VF402_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX7799516.1"
                     /translation="MTYATRFSYLLDKARLLAQARGGLKPPYSGAGRLPPLFFFTDPT
                     RTPHPEDIAAHLPEGCGVVYRHFGEAHAPQRARLLRRIADDNGLTLLIGEDAALAAEI
                     GADGVHLAERSLSRAAVLRSVMPDAVLTIACHDAAALPALSEGVFDAAFVSPVFASRS
                     ASAQGIDALGLKGVQAMTAQTGLPLYGLGGITCDTITQLERSGLAGVAAVDAFRLD"
     gene            3101..>3412
                     /locus_tag="VF402_04150"
     CDS             3101..>3412
                     /locus_tag="VF402_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX7799517.1"
                     /translation="MHKALKAAGRAPDAAVLTAVSKTQPWEAIEPVLRAGHRRFGENR
                     VQEAQKRWGPVRDQYDGLELRLIGPLQSNKAAEAVGFFDVIESVDRDRIARVLADEIQ
                     KQ"
BASE COUNT          604 a         1034 c         1178 g          596 t
ORIGIN      
        1 cgcgcaggcc ggtgtcgaag ccctgctttt cggcggtgtc gatgtcggcc tgggacacgc
       61 cgcccttgtt gagcgaggcg atgaaggcgg cgagggcggg atcacgttcg gcggcggcgc
      121 gcgccagcgg gtgatcggcg gcgatggcga tgaaggcggc gccgaacagc gtgtccgggc
      181 gggtggtgta gacctcgatc ccgtcggcga agccttcggg cgcggcgccg tcgaaggccc
      241 agcgcatctt caggccgcgc gacttgccga tccagttgac ctgcatggcg cgcaccttgt
      301 cgggccagcg ctccagcgtg gtcagcccgt ccagcaggtc gtcggcgaag cgcgtgatgc
      361 gcatgaacca ctgggtcagc ttcttctttt cgaccacggc gccggagcgc cagccgcgtc
      421 cgtcgatcac ctgctcgttg gccagcacgg tcatgtcgac cgggtcccag ttgacctggg
      481 attcgcggcg atagacgaga tcgtgcttca ggaggtcgag aaaccacttc tgctgctggc
      541 cgtaataggc cggatcgcag gtggcgaatt cgcgcgtcca gtcgatcgag aggccgaggc
      601 gctgcaactc gccgcgcatg ttggcgatat tggcgtaggt ccagcccttc gggtgaatcc
      661 tgcgctccat ggcggcgttt tcggcgggca tgccgaacgc gtcccagccc atcggatgca
      721 gcacgttgaa gccgcgcgcg cgcttgaagc gcgccacgag atcgcccatc gcatagttgc
      781 gcacatggcc catatggatg cgccccgacg gataggggaa catctcaagc acgtagtatt
      841 tgggtttttg cggatcagcc ttggtgagga acgtgttcgc cgccgcccac cgcgccagct
      901 ggcggggttc ggcttctttc ggattgtagc gcgccattta aaggtcagga tgatccggtt
      961 gagggttgat tatttcacgt ccgacaggcg cagttcgcgc gccttggaca ggatggcgtt
     1021 ctcgatgtcg acctgggtct gctgcgacac gtcggcgttg gtccactggc cgttctgctg
     1081 cacctgctta tagaccgaga cgctgagcgc gtcggcgcgc aggcgcgagt cgaggatata
     1141 gacggtgatc ttgaagcgct cgtccggctt ttgcgggttg gcgtaccagt cggtgatgat
     1201 cgtgccgccc cacggatcgg ccgaggccag cggcatgaag gagatggtgt cgagcgaggc
     1261 gcgccacaga taggcgttga cgccgatgcc ggcctgcgcg ttgtagtcgt tcttcttggc
     1321 gtggccgccg ccgaacaggt gcgacaacgg cgtgccggtt tccggttcgg tggtgttggc
     1381 agatgcaggc gcggaaggac cgccgggctt gccgcccagg ctggcgcagg ccgtgaagct
     1441 caggcaaacg ccgagcgcaa ccgtcgccag ggccgtttta cgcatcattg tcatcaaggg
     1501 atcgctccgc aaagtttgtc cgaattccgc gcgcgttcgc cgccgcgaaa gtctttagat
     1561 ttctcgccct tgccttaaaa tccccggcct ggctcccggt ctctatagca tgcgggtcag
     1621 ggggtgtgac aagagggcgc tcacaagttt gtcaactcaa cagcaaatat gggcggatat
     1681 gcggcgaagg atcaccgcat ggcgcggaat ttgcttcgga aacgacataa gccaacgcgc
     1741 aggcgcggcg ccagcgtggc ggaatcacca catccgtgag gggaaaaata gcaggggcgc
     1801 gattcatcac tatatgtgtt tgacccgacg cgggtttgcc cttaatcgga ttttgacgct
     1861 tgtgtctgaa aatgtccgcg taagcgtttg tgctgatgag cgtaaatcgc ccgatcgagg
     1921 cgacgtttag gtgttgagac gatgaaacct tcggtgtccc tgttgctgag cgcgacggtt
     1981 ctggccctgg ccggaacgtc gggacatgcg ctggccgtca caccggtgca aaagaccgtc
     2041 cacgcccagc aaggcgctgc gaccggcggc ggactgaacc tggccccgat ctcgcaggac
     2101 agctatatca gcgattcgac gaccagcttc agcaaaaacc cgtccaagac ctatcagttc
     2161 gatctgagca acaaatgggg cgtgcgcttc gacgtcaacc agacggaaac cagcccgtcg
     2221 ggcctgaacg acatcgacgc gggcgccttc tacaagctga ccccttcttt acgcgtcggc
     2281 ggcagcctcg gcttcggcga aaagaccgat ccgaacaaga ccgaagcggc caacgccgcc
     2341 gtggcgcagg acgcccggaa gcagccgcgc gtccggctcg aaaccacctt taaattctaa
     2401 tccagcctga acgcatcgac cgccgcgacg cccgccagcc cggagcgctc cagttgcgtg
     2461 atcgtgtcgc aggtgatccc gccgaggccg tacagcggca ggccggtctg cgccgtcatc
     2521 gcctgaacgc ccttgaggcc cagcgcgtcg attccttgcg ccgaggcgct gcggctggcg
     2581 aagaccggcg agacgaaggc ggcgtcgaag acgccttcgg acagcgcggg caaggcggcc
     2641 gcgtcatggc aggcgatggt caggacggcg tccggcatga cggagcgcaa gacagcggcg
     2701 cggctcaggc tgcgttcggc cagatgcacg ccgtcggccc cgatctcggc ggccagcgcg
     2761 gcgtcctcgc cgatcagcag ggtcaggccg ttgtcgtcgg cgatccggcg caagagccgg
     2821 gcgcgctgcg gcgcgtgggc ctcgccgaaa tggcgataga cgacgccgca gccttcgggc
     2881 aggtgggcgg cgatgtcttc gggatgcggc gtgcgcgtgg ggtcggtgaa gaaaaacagc
     2941 ggcggcaggc ggccggcgcc tgaataaggc ggcttcaatc cgccgcgcgc ctgcgctaga
     3001 aggcgggcct tgtcgagcag ataggaaaag cgggtcgcat atgtcattgc ccgaaatctc
     3061 atccgatacc cctccggcct actctaggct gattgcgaag atgcacaagg cgcttaaagc
     3121 cgccgggcgc gcgccggacg cggccgtttt gacggcggtg tccaagaccc agccgtggga
     3181 agcgatcgaa ccggtgctga gggccggtca tcgccgcttc ggcgagaacc gcgtgcagga
     3241 ggcccagaag cgctgggggc cggttcgcga tcaatatgac gggctggagc tgcggctgat
     3301 cgggccgttg cagagcaaca aggcggcgga ggcggtcggc tttttcgacg tgatcgagag
     3361 cgtggaccgc gacaggatcg cgcgcgttct ggccgacgag atacagaagc ag
//
LOCUS       DASVLL010000252         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_228886,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000252 DASVLL010000000
VERSION     DASVLL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228886"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VF402_04155"
     CDS             complement(<1..872)
                     /locus_tag="VF402_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799518.1"
                     /translation="MPDKPAADDSAKLKHNLWPASVMLALGFSSGLPYLLIYDTASVW
                     LRQAGVSLTDIGYFFLFTTFSYTLKFMWAPVIDRLNLPFLTRRLGHRRGWLLLIQGLL
                     VAGLIGISLSRPERGITGMAIIASLTGFIAATQDIVMDAWRIEAVEEKLQGLMASCYQ
                     WGYRIALILSGVLPLVLAGPLGWNAAYAIMAAFMAVGIAAVLLAPREKAHTIRPIHME
                     GLPSHPWREALEWTARGVLIVLAAILMGCGLTGNADALDFLLGHAGLSEAAQAALKAA
                     WTAKTTGVFLQIPA"
     gene            complement(926..>1958)
                     /locus_tag="VF402_04160"
     CDS             complement(926..>1958)
                     /locus_tag="VF402_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799519.1"
                     /translation="VELSDQGLIRFCVRDTGAGIPEEARARIFEEYGQVQASDATRYG
                     GAGLGLVVVKKLVEVMDGQVTLDSEVGRGSCFTVEFRAPYGLRKAAPTRRLPHAATLV
                     TQNPVLRDGAHSHLGDAGCRLRPAASLDDINDPQGVVLCDRAALDGLVKAPSPNSLIL
                     LAPEQRDEIDGWRAQGWAGYLIKPLRRVSLLERLAALHDRADMPPAEDERIASVSAPN
                     VSVLLVEDNPVNALLAQILLQREGCRVERASSGEEALEIAAKRGFDLIFMDLGLPGLD
                     GLGATRALRARGVSTPVIALTANAYEEDRRASLSAGMNDFLTKPIEVAALRHRLQTWT
                     GAPTKAKSA"
BASE COUNT          298 a          679 c          687 g          294 t
ORIGIN      
        1 aaggccggga tttgcaggaa aacgccggtg gtcttggcgg tccaggcggc tttcagcgcg
       61 gcctgcgccg cttcgctcag gcccgcatgg ccgagcagga agtccagcgc gtcggcgttg
      121 ccggtcaggc cgcagcccat caggatcgcc gccagcacga tcagcacgcc gcgcgccgtc
      181 cattccagcg cctcgcgcca cggatgcgac ggcaggccct ccatgtggat cggacggatg
      241 gtgtgcgcct tttcgcgcgg cgccagcagc accgccgcga tcccgaccgc catgaaggcc
      301 gccatgatcg cataggccgc gttccagccc agcggcccgg ccagcaccag cggcagcacg
      361 cccgacagga tcagcgcgat gcgatagccc cactgataac acgacgccat cagcccctgc
      421 agcttctctt cgaccgcctc gatgcgccag gcgtccatca cgatgtcctg cgtcgcggcg
      481 atgaagccgg tcagcgaggc gatgatcgcc atgccggtga tgccgcgttc ggggcggctg
      541 agcgaaatgc cgatcaggcc cgcgaccagc aggccctgaa tcagcagcag ccagccgcgc
      601 cggtggccga gacggcgcgt caggaacggc aggttcagcc ggtcgatcac cggcgcccac
      661 atgaatttca gcgtgtagct gaaggtcgtg aacaggaaga aatagccgat gtcggtcagc
      721 gacacgcccg cctgccgcaa ccagacgctg gccgtgtcgt agatcagcag atagggcagg
      781 ccggacgaga agccgagcgc cagcatcacc gaggcgggcc agagattgtg cttcagtttc
      841 gcgctgtcgt ccgccgcggg tttgtccggc atgtcgttat ggctccgtct tcggccggga
      901 cggtatggag ctttgcggct ttcgtctagg cggatttcgc ctttgtgggc gcgccggtcc
      961 aggtttgcag ccggtggcgc agggcggcga cctcgatcgg cttggtcaga aaatcgttca
     1021 tgcccgcgct caggctggcg cggcggtcct cctcgtaggc gttggcggtc agggcgatga
     1081 ccggcgtgct gacgccgcgc gcgcgcaagg cccgcgtcgc gccgagtccg tcgaggccgg
     1141 gcaggccgag gtccatgaag atcaggtcga agccgcgttt ggccgcgatc tccagcgcct
     1201 cctcgccgga cgaggcgcgc tcgacgcggc agccctcgcg ttgcagcagg atctgcgcca
     1261 gcagcgcgtt gaccggattg tcctcgacca gcagcaccga aacgttcggc gccgagacgc
     1321 tggcgatgcg ctcgtcctcg gcgggcggca tgtcggcgcg gtcgtgcagc gcggccagac
     1381 gctccagcag cgacacgcgg cgcagcggct tgatcaggta accggcccag ccctgcgcgc
     1441 gccagccgtc gatttcgtcg cgttgctccg gcgccagcag gatcaggctg ttcggcgacg
     1501 gcgccttgac gaggccgtcc agcgcggcgc ggtcgcacag caccacgccc tgcggatcgt
     1561 tgatgtcgtc cagcgacgcg gcggggcgca ggcggcagcc ggcgtcgccg aggtggctgt
     1621 gcgcgccgtc gcgcagcacc gggttttgcg tgaccagcgt tgccgcgtgc ggcaggcggc
     1681 gcgtcggcgc cgccttgcgc aggccgtagg gcgcgcggaa ctccacggtg aagcaggagc
     1741 cgcgcccgac ctcggaatcc agcgtgacct gaccgtccat gacctcgacc agcttcttga
     1801 ccacgacgag gccgaggccc gcgccgccat agcgtgtggc gtcgctggcc tgcacctggc
     1861 cgtattcctc gaagatgcgc gcgcgcgcct cctccgggat gccggcgcct gtgtcgcgca
     1921 cgcaaaagcg gatcaggccc tgatcgctca gctcgacg
//
LOCUS       DASVLL010000253         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_389928,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000253 DASVLL010000000
VERSION     DASVLL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389928"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VF402_04165"
     CDS             <1..182
                     /locus_tag="VF402_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7799520.1"
                     /translation="AEKAYRVSAVKTGSGDTYAYSHTSVVYLMNPEGQFAVPLTAGMT
                     AQQNADQIRQAMQEG"
     gene            complement(179..382)
                     /locus_tag="VF402_04170"
     CDS             complement(179..382)
                     /locus_tag="VF402_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7799521.1"
                     /translation="MIRVRLAEALAKRRLSIVELSDRTGIGVRDLEILRDGRALAVRL
                     STLDALCRALGCAPGDLLDYDGD"
     gene            complement(379..900)
                     /locus_tag="VF402_04175"
     CDS             complement(379..900)
                     /locus_tag="VF402_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX7799522.1"
                     /translation="MRLLGPRSIASLLKTALDVVYYGLYTLIFGAAAAVICVLSVPNL
                     AAEVVRRMNIGADLSAGSQAVMLIAFAVSLGGYLMVMRWTRAIFRTLAEGDVFHPDNT
                     VRLRSIGVALAALEVFGYAARAVASGFFHIEFEPIYGLRAVTMWFLVLVVFVLAEVFK
                     EGARLRREADLTI"
     gene            1153..1767
                     /gene="rdgB"
                     /locus_tag="VF402_04180"
     CDS             1153..1767
                     /gene="rdgB"
                     /locus_tag="VF402_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX7799523.1"
                     /translation="MSLSLIPGQKLIAATHNPGKAREISALLDGRFEVIPAGGLGLPE
                     PPETEVSFIGNAILKARHAAMTAGLTALADDSGLSIAALDGDPGIYSARWAGPYKDFD
                     RAMEVIEHKLRLAKDARGDGYTTRAWFTCALAVAWPEGHACVFEGRVDGEIVFPKRGG
                     KGFGYDPIFRPDGHDPTFAEMDPQAKDAISHRHLAFEQLRKALF"
     gene            1768..3006
                     /gene="hemW"
                     /locus_tag="VF402_04185"
     CDS             1768..3006
                     /gene="hemW"
                     /locus_tag="VF402_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7799524.1"
                     /translation="MEPLALYIHWPYCSRICPYCDFNVTRDRKQASQRALFDAMLADL
                     RRQSQGLGPRRLSSVFFGGGTPSLLPPDWVAELIGAAKALFPISGNMEITLEANPTDA
                     EIARYEGFRAAGVNRLSLGVQALDDDALRFLGRNHSAREAMQGVETAQAVFGRVSLDL
                     IYALPGQTPDMWEAELRRAAALGVEHVSPYQLTVEPGTAFGRAARRGAWSPPDQDMAE
                     ALYDTTQATLGALGFEAYEVSNHARGPAARSAHNVAVWEGVDYMGIGPGAHGRLTRPV
                     ATDVQARQNKDSAHGRLTRPVATDIQARQNENPAHGRLTRGGFRYATVAESDLKAYTA
                     AADPIASAALSAQESEEEALMLGLRLIDGVRLTRAPNLNRAKADALIADGFLERTPER
                     LRATDKGRVVLDRLLLELLS"
     gene            3026..3229
                     /locus_tag="VF402_04190"
     CDS             3026..3229
                     /locus_tag="VF402_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799525.1"
                     /translation="MMIYAAFFGLGLLLCIGLTTGWLDRTVPHGAPVAVIGFAAAWAI
                     LRYGVARRHFPGGWRGKRKDRPE"
     gene            3226..>3936
                     /gene="rsmI"
                     /locus_tag="VF402_04195"
     CDS             3226..>3936
                     /gene="rsmI"
                     /locus_tag="VF402_04195"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX7799526.1"
                     /translation="MTETLFAPPPKPVGPGLYIVATPIGNLRDITLRALDVLRAADVV
                     LAEDTRVAGKLLAAYDLKKKLIRYDDHAGPQALPEILDRLRAGEVIAQISDAGTPLIS
                     DPGFRLVAEARRAGLRVHPIPGASSVLAALCLSGLPTDRFLFAGFIPNKSAARKSFFA
                     GFAAQAATLVFFETGPRLRDSLSDMLGVLGDREACVCRELTKLYETGHGGRLSGLAAD
                     PALHEPKGEIVIVVAPPEA"
BASE COUNT          648 a         1373 c         1325 g          590 t
ORIGIN      
        1 aggccgagaa ggcgtacagg gtcagcgcgg tcaagaccgg ctccggcgat acatacgcct
       61 acagccacac ctcggtggtt tatctgatga acccggaggg ccagttcgcg gttccgctga
      121 ccgccggcat gaccgcgcag cagaacgccg accagatccg gcaggcgatg caggaaggct
      181 aatccccgtc gtaatccagc aggtcgcccg gcgcgcagcc cagcgcccgg cacaaggcgt
      241 ccagcgtcga caggcgcacg gccagcgccc tgccgtcgcg caggatctcc agatcgcgca
      301 cgccgatccc ggtgcggtcg gacagctcga cgatcgacag gcggcgcttc gccagcgcct
      361 cggcgagacg gacgcggatc atatggtcag gtccgcttcg cggcgcaggc gggcgccttc
      421 cttgaacacc tcggccagca cgaacaccac cagcacgagg aaccacatgg tcaccgcgcg
      481 caggccgtag atcggctcga actcgatgtg gaagaagccc gacgccacgg cgcgcgccgc
      541 atagccgaac acctccagcg ccgccagcgc cacgccgatg gagcgcagcc gcacggtgtt
      601 gtccgggtga aacacgtcgc cctcggccag ggtgcggaaa atcgcccgcg tccagcgcat
      661 gaccatcaga tagccgccca gcgagaccgc gaaggcgatc agcatcaccg cctgcgaacc
      721 ggcggacaga tcggcgccga tattcatccg ccgcaccacc tcggcggcca ggttgggcac
      781 gctcagcacg cagatcaccg ccgccgccgc gccgaagatc agggtgtaaa gcccgtaata
      841 gacgacgtcg agcgcggtct tcagcaggct ggcgatggag cgcggaccta acaggcgcat
      901 ggatttttcc ccggtgacgc gccgccggac cgggcggcgg ttcaggtttt ctagccgcga
      961 aaagcgcgaa aggacacgaa aaaagatagc cgctttcacg gacgcgagaa aaatcggttt
     1021 ggcccggctg cgcctgtgcg tccgtgacga tgacgttcgg cgccattttc gtgtcccttc
     1081 gtgtctttcg cggccgaacc tctttgtctt ttgcacggtt gccgctagaa ccaccgtcac
     1141 aaggagcgcc tcatgagttt gtccctgatc cccggccaga agctgatcgc cgccacgcac
     1201 aatcccggca aggcgcgcga gatttccgcc ctgctggacg gacgcttcga ggtgatcccg
     1261 gcgggcgggc tgggcctgcc cgaaccgccg gaaaccgagg tcagcttcat cggcaacgcc
     1321 atcctgaagg cgcgccatgc ggccatgacc gcgggcctga ccgcgctggc cgacgattcc
     1381 ggcctgagca tcgcggcgct ggacggcgat ccggggattt attcggcgcg ctgggccggg
     1441 ccttacaaag atttcgaccg cgcgatggag gtgatcgagc acaagctgcg gctggcgaag
     1501 gacgcccggg gcgacggcta tacgacgcgc gcctggttca cctgcgcgct ggcggtggcg
     1561 tggccggaag ggcacgcctg cgtgttcgag ggccgcgtcg acggcgagat cgtgtttccg
     1621 aagcgcggcg gcaagggctt cggctacgac ccgatcttcc gccccgacgg ccacgacccg
     1681 accttcgccg agatggaccc gcaggccaag gacgcgatca gccaccgcca tctggccttc
     1741 gagcagttgc gcaaggcgtt gttctaggtg gagccgctcg ctctctacat ccactggccc
     1801 tactgttcgc gcatctgccc ctattgcgat ttcaacgtca cgcgcgaccg taagcaagcc
     1861 agccagcgcg cgctgttcga cgccatgctg gccgacctgc ggcggcaatc gcaagggctg
     1921 gggccgcgcc ggctgagcag cgtgtttttc ggcggcggca ccccgtcgct gctgccgccg
     1981 gactgggtcg cggagctgat cggcgccgcg aaagctctgt tcccgatttc cggcaatatg
     2041 gaaataacgc tcgaagccaa tccgaccgac gccgagatcg cgcgctacga aggcttccgc
     2101 gcggcgggcg tcaaccggct gtcgctgggc gtgcaggcgc tggacgacga cgcgctgcgc
     2161 tttctcggcc gcaatcattc ggcgcgcgag gcgatgcagg gcgtcgagac ggcgcaggcc
     2221 gtgttcgggc gcgtgtcgct ggacctgatc tacgccctgc ccggtcagac gccggacatg
     2281 tgggaggcgg agctgcgccg cgccgccgcg ctgggcgtcg agcatgtctc gccctatcaa
     2341 ctgaccgtcg aacccggcac ggccttcgga cgcgcggcgc ggcgcggcgc gtggtcgccg
     2401 cccgatcagg acatggccga agcgctttac gacacgacgc aggcgacgct cggcgcgctc
     2461 ggcttcgagg cttacgaggt gtcgaaccat gcgcgcggcc ccgccgcccg ctcggcgcac
     2521 aatgtcgccg tctgggaggg cgtcgactat atgggcatag gccccggcgc gcatgggcgg
     2581 ctgacgcgac ccgtcgcgac agatgtacag gcgcgccaaa acaaagactc cgcgcatgga
     2641 cggctgacgc gacccgtcgc gacagacata caggcgcgcc aaaacgaaaa ccccgcgcat
     2701 ggacggctga cgcgcggcgg cttccgttac gccacggtcg cggaatccga tctgaaagct
     2761 tataccgccg ccgccgatcc catcgcgtcc gccgccctgt ccgcgcagga gtccgaggag
     2821 gaggcgctga tgctgggttt gcgcctgatc gacggggtgc ggttgacgcg cgcgccgaat
     2881 ctcaatcgcg ccaaggccga cgccctgatc gcggacggtt ttctggaaag gacgccggag
     2941 cgcctgcgcg ccacggataa gggccgcgtc gtgctggaca ggctgctgct ggagcttcta
     3001 tcatagccgc atgactcgca ccgccatgat gatatatgcg gcctttttcg gcctcggcct
     3061 gttgctgtgc atcggcctga ccaccggctg gctggacagg accgtccccc acggcgcgcc
     3121 cgtcgcggtg atcggcttcg ccgccgcctg ggcgattctc cgctatggcg tggcacggcg
     3181 gcattttccc ggcggctggc gcggcaagcg caaagacagg ccggaatgac cgaaaccctg
     3241 ttcgccccgc cgccgaagcc cgtcggcccc ggcctctata tcgtcgccac gccgatcggg
     3301 aacctgcgcg acatcaccct gcgcgcgctc gacgtgctgc gcgccgccga cgtggtgctg
     3361 gccgaggaca cgcgcgtggc gggcaagctt ctggccgcct acgacctgaa gaaaaagctg
     3421 atccgctatg acgatcacgc cgggccgcag gcgctgccgg aaattctcga ccgcctgcgc
     3481 gcgggcgagg tgatcgcgca gatttccgac gcgggcacgc cgctgatctc cgatcccggc
     3541 tttcgcctcg tcgccgaggc gcgccgggcg ggcctgcgcg tccatccgat ccccggcgcc
     3601 tcgtcggtgc tggcggcgct gtgcctgtcg ggcctgccga ccgaccgctt cctgttcgcg
     3661 ggcttcatcc cgaacaagag cgccgcgcgc aaaagcttct tcgccgggtt cgcggcgcaa
     3721 gccgccacgc tggtgttttt cgagaccgga ccgcgcctgc gcgacagcct gagcgacatg
     3781 ctgggcgtgc tgggcgaccg cgaggcgtgc gtgtgccgcg aactgacgaa gctgtacgaa
     3841 accggccatg gcgggcgcct gtccggcctc gccgccgacc cggcgctgca cgagccgaag
     3901 ggcgaaatcg tcatcgtcgt ggcgccgccc gaggcc
//
LOCUS       DASVLL010000254         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_229167,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000254 DASVLL010000000
VERSION     DASVLL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229167"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /gene="dgt"
                     /locus_tag="VF402_04200"
     CDS             <1..873
                     /gene="dgt"
                     /locus_tag="VF402_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEX7799527.1"
                     /translation="LGHTPFGHAGEDALDDCLAAFGGFDHNSHGLRLVTELERHYADF
                     DGLNLTWETLEGVVKHNGPVSHRMDEPSWKAIADFNALWDLRPDGFASLEAQCAAIAD
                     DIAYNNHDVDDGLRAGLFSIEDLLEVPMIGAALRSVRADWPDLEPRMLRLEGVRRMIG
                     VMTQDVLAETRKRLAEDRVVTVEDARAARRQMVAFSKPVYDDLSRLRGFLYERMYKHY
                     KINRVRSYAKRTLNEMFQQFMAEPDTLPDAWFQLVCARDTETGRARVVCDYIAGMTDA
                     YAIEEHRRLFSFGG"
     gene            968..2125
                     /locus_tag="VF402_04205"
     CDS             968..2125
                     /locus_tag="VF402_04205"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX7799528.1"
                     /translation="MFSFYEFFAGGGMARAGLGDGWDCLFANDFDARKGLTYQLNWGV
                     GGELRVGDVRAVRADDLPGQADLVWGSFPCQDLSLAGVGAGLKGERSGAFYPFWDVLR
                     GLGAAGRAPKLVVIENVVGALTSHGGQDFQAICRTFAEAGYRYGALVIDAALFVPQSR
                     PRLFVIGVREDMQSDALTACEPLEPFHTRAVRHAVERLPAAARARHAWWNLPAPPHRA
                     DSFADLIEDHPDSVAWHQPDETQRLLAMMSPVNRDKVDAARRAGRRMVGAIYKRTRLN
                     EKGEKVQRAEVRFDDVAGCLRTPAGGSSRQVIMVVEGEAIRSRLISARETARLMGLPD
                     SYRLPRRYNEAYHLTGDGVVVPVVRHLARHLFEPLLGAVGAQAGAGLRQVS"
     gene            2122..2835
                     /locus_tag="VF402_04210"
     CDS             2122..2835
                     /locus_tag="VF402_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7799529.1"
                     /translation="MTLPGPDTIHAPPFETNDLRENLTAYLDTPFTAGDGTTRKVGDF
                     RWGVYAFFDYDREPIYVGQTNERLRTRIRRHLTNQRTDAVAMSVLDPFEVFEIEVWPL
                     PQFEGVKSSDAVARAHLDALERQVTALAVARSRFKAILNEKDPPSGSLSVDMPASFRA
                     RVVSERVFDLRAHPDYRIARRALIISRLAQVISERKVQGGLRRVLLTQAKRLQGLAGR
                     RYEALGGAASVAEHGDDET"
     gene            2807..3226
                     /locus_tag="VF402_04215"
     CDS             2807..3226
                     /locus_tag="VF402_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEX7799530.1"
                     /translation="MRNTATTKPDALRSRTMRAVKSKDTAPELRVRRLVHAMGYRYRL
                     HRKDLPGHPDLVFPGRRKAIFIHGCFWHGHDCRRGARMPKTNADYWTAKIGRNRERDA
                     RTLAELAKLGWGVCVLWECELKDEAALRRTLRAFLDG"
BASE COUNT          520 a         1143 c         1226 g          561 t
ORIGIN      
        1 ctcggccaca cgcctttcgg ccatgccggc gaggacgcgc tcgacgattg cctcgccgcc
       61 ttcggcggct tcgaccacaa cagccacggc ctgcggctcg tcaccgagct tgagcgccat
      121 tacgccgatt tcgacgggct gaacctgacc tgggagacgc tggagggcgt cgtcaagcac
      181 aacggtccgg tgtcgcaccg catggacgag ccgtcgtgga aggccatcgc cgacttcaac
      241 gcgctgtggg atctgcgccc ggacgggttc gcctcgctgg aggcgcagtg cgcggccatc
      301 gccgacgaca tcgcctacaa caatcacgac gtcgacgacg ggctgcgcgc gggcctgttc
      361 agcatcgagg atctgctgga ggtgccgatg atcggcgcgg ccctgcgcag cgtgcgcgcg
      421 gactggcccg atctggagcc gcgcatgctg cggctggagg gcgtgcggcg catgatcggc
      481 gtgatgacgc aggacgttct ggccgagacg cggaagcgtc tggccgagga ccgcgtcgtc
      541 accgtcgagg acgcgcgcgc ggccaggcgt cagatggtgg ccttttcgaa gccggtgtac
      601 gacgacctgt cgcgacttcg cggattcctt tatgagcgca tgtataaaca ctataaaatc
      661 aatcgtgtgc gctcatacgc caaacgcacg ctgaacgaga tgttccagca gttcatggcc
      721 gagccggaca ccctgccgga cgcgtggttc caactggtgt gcgccaggga caccgagacg
      781 gggcgcgccc gcgtcgtgtg cgactacatc gccggcatga ccgacgccta cgccatcgag
      841 gagcatcgcc gcctgttctc ctttggcggt tgacaaacgc gccgccttga gaacaattgc
      901 ggaacattct gttttggcga ttcggcggcc agccgaatca cggcagggtg cgggcatgag
      961 ggcgcagatg ttcagctttt acgaattttt cgcgggcggc ggcatggcgc gcgccgggct
     1021 gggggacggc tgggactgcc tgttcgccaa cgatttcgac gcgcgcaagg ggctgaccta
     1081 tcaactgaac tggggcgtcg gcggcgagtt gcgcgtcggc gacgtgcgcg cggtgcgggc
     1141 cgacgacctg ccgggacagg ccgatctggt ctgggggtcg tttccgtgtc aggatctgtc
     1201 gctggcgggc gtcggcgccg ggctgaaggg cgagcgttcg ggcgccttct atccgttctg
     1261 ggacgtgctg cgcggcctgg gcgcggcggg gcgggcgccg aagctggtgg tgatcgagaa
     1321 cgtcgtcggc gcgctgacct cgcacggcgg ccaggatttt caggccatct gccgcacctt
     1381 cgccgaggcc gggtaccgct acggcgcgct ggtgatcgac gcggcgctgt tcgtgccgca
     1441 gtcgcggccg cgcctgttcg tgatcggcgt gcgcgaggat atgcagagcg acgcgctgac
     1501 cgcgtgcgag ccgctggagc cgttccacac ccgcgccgtg cgtcacgcgg tcgagcgttt
     1561 gcccgcggcg gcgcgcgcgc gccatgcgtg gtggaacctg ccggcgccgc cgcatcgcgc
     1621 cgacagcttc gccgacctga tcgaggacca tcccgacagc gtggcgtggc accagccgga
     1681 cgagacgcag cgcctgctgg ccatgatgtc gccggtcaat cgcgacaagg tcgacgccgc
     1741 gcgccgcgcc gggcggcgca tggtcggggc gatttacaag cgcacgcgcc tgaacgaaaa
     1801 gggcgagaag gtgcagcgcg ccgaggtgcg cttcgacgac gtggccggtt gcctgcgcac
     1861 cccggcgggc ggctcgtcgc ggcaggtgat catggtggtc gagggcgagg cgatccgctc
     1921 gcggctgatt tcggcgcgcg agacggcgcg gctgatgggg ctgccggaca gctaccgcct
     1981 gcccagacgc tataacgaag cctaccacct gaccggcgac ggcgtggtcg tgccggtggt
     2041 gcgccatctg gcgcggcacc tgttcgagcc gctgctgggc gcggtggggg cgcaggcggg
     2101 cgccgggctg cgtcaggttt cgtgactctg cccggacccg acacgatcca cgcgccgccg
     2161 ttcgagacga acgacctgcg cgaaaacctg accgcctatc tcgacacgcc gttcaccgcc
     2221 ggggatggaa cgacgcgcaa ggtcggcgat ttccgctggg gcgtgtacgc cttcttcgat
     2281 tacgaccgcg agccgatcta tgtcggccag acgaacgagc gcctgcgcac ccgcatccgc
     2341 cgccacctga ccaaccagcg caccgacgcg gtcgccatgt cggtgctgga cccgttcgag
     2401 gtgttcgaga tcgaggtctg gccgctgccg cagttcgagg gcgtcaaatc gtccgacgcg
     2461 gtggcccgcg cgcatctcga cgcgctggag cggcaggtga cggcgctggc ggtcgcgcgc
     2521 tcgcgcttca aggccatcct gaacgagaaa gacccgccgt cgggctcgct gagcgtcgac
     2581 atgcccgcat ccttccgggc gcgcgtggtg tccgagcgcg tcttcgacct gcgcgcccat
     2641 cccgattatc gcatcgcgcg ccgcgccctg atcatctcgc gcttggcgca ggtgatttcc
     2701 gagcgtaagg tgcagggcgg tctgcggcgc gtgcttctca ctcaggccaa gcggctgcaa
     2761 gggctggccg ggcgccgcta cgaagcgctg ggaggcgcgg cctcggttgc ggaacacggc
     2821 gacgacgaaa cctgacgccc tgcgctcgcg caccatgcgg gcggtcaagt cgaaggacac
     2881 ggcgccggaa ctgcgcgtgc gccgtctggt tcatgcgatg ggctatcgct accgcctgca
     2941 ccgcaaggat ctgcccggcc atcccgatct ggtctttccg ggccgcagga aggcgatctt
     3001 catccacggc tgcttctggc acggacacga ctgccgccgc ggcgcgcgca tgcccaagac
     3061 caacgccgac tactggaccg ccaagatcgg ccgcaatcgc gaacgtgatg cacgaacgct
     3121 ggcggaactg gcgaagctgg gctggggcgt ctgcgtgctg tgggagtgcg aactgaagga
     3181 cgaagcggcg ctgcgccgga ccctgcgcgc ttttctcgac ggctgaaggc ttaccgcatc
     3241 ctgatttttc ccgtgcgaag cgggcatcgc atcgtcacga ggctttacct atcctgccgc
     3301 cctgtctgca atcgatcttc aatcccggca cggcggaggt ttccatgttc aagctcaggt
     3361 tcaacggcgc ggctctggcc ttcgcgctct gcacggcggc gatggcgccg gccatgctgc
     3421 cgacggcggc cgaggccgcg accaaggccg
//
LOCUS       DASVLL010000255         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_499735,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000255 DASVLL010000000
VERSION     DASVLL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499735"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VF402_04220"
     CDS             complement(<1..765)
                     /locus_tag="VF402_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799531.1"
                     /translation="MFNNRRALTLRGGASLLALTALTALAFAGGAQAQDAATKAKDDA
                     TEVVVVGVRKALQSAQQVKKNADTVVDSITATDIGAFPDKSVAEALQRVPGITVTRFA
                     ASDDSSHFSAEPSGVLIRGLTQVRTEFNGRDSFSADGARGLNFADIAPELMSRVDAYK
                     NQTADMIEGGIAGTVDLRTRLPFDSKGQVLSISLKGNYGDISKKSTYDYSGLYSNTWD
                     TSLGQFGLLADYANSHVITATNGVIMGRIATICSAGY"
     gene            complement(1108..1791)
                     /locus_tag="VF402_04225"
     CDS             complement(1108..1791)
                     /locus_tag="VF402_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7799532.1"
                     /translation="MRYDAEHKERTRLRLLTEAAIMLREEGPHGLSVATLMKRQGLTH
                     GGFYAHFASKDDLIAQAIDVMFERTCERFETRTRGLAPQEALAGYIAYYLSPAHCNKP
                     GQGCAIPATAGDVARLEPEARKRFEAGASALQRLIARQFEQAGFGAAEALNQAAALLS
                     ELSGAVMMARAVKSPHMQKQICASAHSAILQRLRRYWPDFGAKAQAEVEAAFAPLLSE
                     DEDALSVSV"
BASE COUNT          313 a          686 c          625 g          357 t
ORIGIN      
        1 gtagcccgcc gagcagatgg tggcgatgcg gcccatgatc acgccgttgg tggcggtgat
       61 gacgtgcgaa ttggcatagt cggccagcaa gccgaactgg ccgagcgaag tgtcccaggt
      121 gttggagtaa aggccggaat agtcgtaggt cgacttcttg gagatgtcgc cgtaattgcc
      181 cttcaggctg atcgacagca cctgcccctt ggaatcgaac ggcaggcgcg tgcgcagatc
      241 gaccgtaccg gcgatgccgc cctcgatcat gtccgccgtc tggttcttgt aggcgtcgac
      301 gcggctcatc agctccggcg cgatgtcggc gaagttcagc ccgcgcgcgc cgtccgccga
      361 gaacgagtcg cggccgttga actcggtgcg cacctgcgtc aggccgcgga tcagcacgcc
      421 cgacggctcc gccgaaaagt gcgaggaatc gtcggaggcc gcgaagcgcg tcacggtgat
      481 gcccggcacg cgctgcaacg cctcggccac cgacttgtcg gggaaggcgc cgatatcggt
      541 ggcggtgatc gaatcaacga ccgtgtcggc gttcttcttg acctgctgcg cgctctggag
      601 cgccttgcgc acgccgacga ccaccacttc ggtcgcatca tccttcgcct tcgtcgccgc
      661 gtcctgcgcc tgcgcgccgc cggcaaacgc cagcgccgtc agcgccgtca gcgccagcag
      721 cgatgcgccg ccgcgcaacg tcagcgcccg tctgttgttg aacatctgca tatcctctcc
      781 cttgaggctc cctaaaggcc ccgtctggct tgttgtccgt cagttacggc gcccgcccgc
      841 cgcacgcggc gccggcgatc gccgtgtgac ccggcccgtt cgaggggccg cccgcacgcc
      901 ttctcaaccc cgcaggccca gggcccgccc tttccgtccc gtcatttttt tcgcatcccg
      961 gtttttcgct catgggatga tgacctgcat atcaattgca tgatgatcat cattcaatca
     1021 agcgcggaaa cgtcgtcatg gccgatgcat gacggaaaac cggcgtcttt tgcaaaaatg
     1081 caacaatccg gcgcgcggaa aagcgcttca gacgctgacg gacaaagcgt cctcgtcctc
     1141 ggacagcagc ggcgcgaacg ccgcctcgac ttccgcctgc gccttggcgc cgaagtcggg
     1201 ccagtaacgg cgcaaccgtt gcaggatcgc cgaatgggcg ctggcgcaga tctgtttctg
     1261 catgtgcggc gacttgaccg cgcgcgccat catcaccgcc ccgctcagtt cgctcagcag
     1321 cgccgccgcc tgattcagcg cctcggcggc gccgaagccc gcctgttcga actgccgcgc
     1381 gatcaggcgt tgcagcgccg acgccccggc ctcgaaccgc ttgcgcgcct caggctccag
     1441 ccgcgccacg tcccccgcgg tggccgggat cgcgcacccc tgccccggct tgttgcaatg
     1501 cgcgggcgac agataatagg cgatgtatcc ggccagcgcc tcctgcggcg ccagcccgcg
     1561 cgtgcgcgtc tcgaaacgct cgcacgtgcg ctcgaacatc acgtcgatgg cctgggcgat
     1621 cagatcgtcc ttggaggcga aatgcgcgta aaacccgccg tgcgtcaggc cctgccgctt
     1681 catcagcgtg gcgacgctga ggccgtgcgg cccttcctcg cgcagcatga tcgccgcctc
     1741 ggtcaacaga cgcaggcgcg tgcgctcctt gtgttcggca tcgtaacgca tgaacgggcc
     1801 ttcgcggttt gctccgccgc gagtcttccc gttttcccct gccgcgtcaa gagcggggtt
     1861 atctttaagg ggcgacagcc gcggcgccgt ctgctaaaga gaacgtggtt aactggaacg
     1921 aggacggggg gagttcaacg tgtcgcgcct gctgggactg gccttgctcg tgatcgtcgc
     1981 g
//
LOCUS       DASVLL010000256         7482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_176733,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000256 DASVLL010000000
VERSION     DASVLL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176733"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VF402_04230"
     CDS             complement(<1..364)
                     /locus_tag="VF402_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HEX7799533.1"
                     /translation="MSDEPFDKRTTPYKDGVTDQAHEGLIRARSYVAGEVLSCYDATA
                     PILAQPEHGAEQWDQLLMGERFKVIERKHDFYWGQALRDGYVGYAPVSAFRRDWYLPT
                     HYVATLRTYVFAAPNLKAS"
     gene            complement(468..1856)
                     /locus_tag="VF402_04235"
     CDS             complement(468..1856)
                     /locus_tag="VF402_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX7799534.1"
                     /translation="MPKPLKTPAPAANRLIAVFEEEAESVVGLAAEADFLRLKGFSGK
                     AGSLIALPGKSGLTAWFGLGARRNYNPMSFRALPPQLPRGRWRLEADQGLDRAAIHAA
                     WGFGAYAFTRYKAAPKPVEAVLDDSELDAATRAAVAAEVAAHARARDLVNTPTNDMGP
                     AQIEAAARDIAETHGAAIRVVTGDDLLKENYPAVHAVGRAAGPGNAPRFIEMTWRSNP
                     VQKDAPVIVLIGKGVAFDTGGLNIKPGSGMALMKKDMGGAAHALALGDWVMASNLPVQ
                     LHILIPAVENSISGDAFRPGDVLASRAGVSIEIGNTDAEGRLILADALTRAGELNPTL
                     SVDFATLTGAARVALGPEVIPFYTEDEDLARRLEVASIARHDPLWRMPLWAGYKDALE
                     SDIADIRNDPAGWAQGGSVTAALFLQRFAPGVGAWVHFDIYAWNPRGRPGHPVGAEAQ
                     ALRAVYEVLKAY"
     gene            1954..2889
                     /locus_tag="VF402_04240"
     CDS             1954..2889
                     /locus_tag="VF402_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7799535.1"
                     /translation="MNRHFRLAVRAACCALAAGGLAAGPASAGLFGKTQDAPAVAPAV
                     SGPTQTSVSTNLPVMAGTGGADDGFHKASKDEIAAALRADPLAQAAFFTNQFDHAPDD
                     PQMGLYLSNALRALGRYDQAADIAHRVLLFAPDNVDLLLAAARAHIAGDDAFLAVAPL
                     QHVIELRPKDWTAHSLLGVAYQQIKRPDDAQGEWATALKLSPDNPDVLTNIAMAKAAG
                     GDLAGAETLLRDAAAQKDATTQVRQNLALVLGLQGKLAEAERLLRQDLPPQQADADLA
                     WLQQAAAARNHAPAAAPAAVESPTRSWASVQAAGG"
     gene            3124..4176
                     /locus_tag="VF402_04245"
     CDS             3124..4176
                     /locus_tag="VF402_04245"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX7799536.1"
                     /translation="MSSSILLADSQAGLLKASHAYKPFRYPWAFEFWRKQQQVHWIPE
                     EVPLGEDCKDWAAKLNDGERNLLTQIFRFFTQSDIEVQDNYLERYSRVFKPTEVKMML
                     SSFANMETIHIAAYALLLETIGMPESEFGAFMDYQAMRDKHDYMQDFGVDTEADVCRT
                     LAMFGGFTEGLQLFASFAMLMNFPRFNKMKGMGQIVSWSVRDESLHCEGIIKLYHAFS
                     RETGAVTRSVADDIIDCCKTVVTMEDKFIDLAFEMGPVQGMTAEDIKAYIRYIADWRL
                     KQLELPQVFDAPKENPLPWLQVLLSGVEHANFFEARATEYSKAATKGQWTGDAGVWGA
                     FDQLMKKRAEGMKANA"
     gene            4558..4890
                     /locus_tag="VF402_04250"
     CDS             4558..4890
                     /locus_tag="VF402_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX7799537.1"
                     /translation="MSIQDVGAQAARGPVFFERAELNLILRLYGQQVASGEWRDYAID
                     QFPDAVSFNIYRRSSEAPLYRIEKRPALARKQGAFAVFNQAGLILKRGRELGPVLNVL
                     DKRRFDVL"
     gene            complement(5004..5657)
                     /gene="fsa"
                     /locus_tag="VF402_04255"
     CDS             complement(5004..5657)
                     /gene="fsa"
                     /locus_tag="VF402_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX7799538.1"
                     /translation="MLLFADTADTKVLAELKETGLIDGVTTNPTIIAKSGRNMFEVIK
                     EICELIDGPISAEVAATDAPTMIREGEKLAKIASNVVIKVPLTIEGLKATREFSAQGL
                     MTNVTLCFSVNQALLAAKAGATYVSPFVGRLDDQGADGMQLIHDIRQIYDNYDFDTEI
                     LAASLRNTAHIAQAALAGADCATLPPQAFKDMFKHPLTDKGLETFLADWAKTGQSIL"
     gene            5766..7181
                     /gene="rimO"
                     /locus_tag="VF402_04260"
     CDS             5766..7181
                     /gene="rimO"
                     /locus_tag="VF402_04260"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX7799539.1"
                     /translation="MTTSSSAPSVGFVSLGCPKALVDSERILTRLRGEGYATSASYGG
                     ADVVVVNTCGFLNSAKEESLAAIGEALNENGKVIVTGCMGGEADLIRQRFPEVAAITG
                     AHQYDAVMDAVHNIVPPKPDPFRPLIPESDPGVHLTPKHYAYLKISEGCDHRCTFCII
                     PSLRGDLASRPVAEVLREAEKLAQVGVKELLVVSQDTSAYGLDIKYAQSEWRGRNWDA
                     SFEDLSRGLGELGVWVRMHYVYPYPHVNAVIPLMAEGKILPYLDIPFQHASPKILKLM
                     KRPGNQDRTLQRIESWREICPDLTLRSTFVVGFPGETEADFNLLLDWLEVAQLDRVGA
                     FAYENVTGAAAQGLPDHVPEEVKQDRLARFMAVASRISAQKLQNKVGRVEDCLIDDLR
                     NDGTAIARTRGDAPEIDGHIFLKGFTGLKPGDLVKAKVTRADAYDLWGEPEGLIKLNR
                     VPGQLSGAAQKRRMHTLISRI"
     gene            complement(7225..>7482)
                     /locus_tag="VF402_04265"
     CDS             complement(7225..>7482)
                     /locus_tag="VF402_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7799540.1"
                     /translation="RIMAECGADPLHAALSYALNIEGASTAIVGVASAAELRAIIAAS
                     ERPAPRIPWESLALNDEVALDPAQWFADLGERTQRRLVRVA"
BASE COUNT         1319 a         2471 c         2415 g         1277 t
ORIGIN      
        1 tcgaggcttt caggttcggc gcggcgaaca cataggtgcg cagggtggcg acatagtgcg
       61 tcggcagata ccagtcgcgg cggaaggccg acaccggcgc atagccgaca tagccgtcgc
      121 gcagcgcctg accccagtag aagtcgtgct tgcgctcgat caccttgaag cgctcgccca
      181 tcagaagctg atcccactgt tcggcgccgt gttcgggctg ggccaggatc ggcgccgtgg
      241 cgtcgtaaca gctcagcacc tcgcccgcga cgtaggagcg cgcgcggatc aggccctcat
      301 gcgcctgatc ggtcacgccg tccttgtagg gcgtcgtgcg cttgtcgaaa ggttcgtcgc
      361 tcatttgcgt catggaagct tctgccttca ccagtcctat ggacagcaga ctagcgcagg
      421 gatgcaaaag tcgcgttaac ggccacggtc aatgaagtgt tgatgcgtca gtacgctttc
      481 aagacctcgt aaaccgcccg cagcgcctgc gcctcggcgc cgaccggatg gccgggccgc
      541 ccgcgcgggt tccaggcgta gatgtcgaaa tggacccagg cgccgacgcc gggcgcgaag
      601 cgttgcagga acagggcggc ggtcaccgac ccgccctgcg cccagcccgc cggatcgttg
      661 cggatgtcgg cgatgtcgga ctcgagcgcg tccttatatc cggcccagag cggcatgcgc
      721 cacaaaggat cgtggcgcgc gatcgacgcg acttcgaggc ggcgggccag atcctcgtcc
      781 tcggtgtaga aggggatgac ctccggcccc agcgccacgc gcgccgcgcc ggtcagggtg
      841 gcgaaatcga cgcttagggt cgggttcaac tcgcccgccc gcgtcagcgc gtcggccagg
      901 atcagccgcc cttcggcgtc ggtgttgccg atctcgatgc tgacgcccgc gcgcgaggcc
      961 agcacgtcgc cggggcggaa ggcgtcgccg gagatggagt tctccaccgc cgggatcagg
     1021 atgtgcaact gcaccggcag gttggacgcc atcacccagt cgcccagagc caaagcgtgc
     1081 gcggcgccgc ccatatcctt cttcatcagc gccatgcccg aaccgggctt gatgttcagc
     1141 ccgccggtgt cgaaggcgac gcccttgccg atcagcacga tcaccggcgc gtccttctgc
     1201 acgggattgc tgcgccatgt catctcgatg aagcgcggcg cgttgcccgg ccccgccgcg
     1261 cgcccgaccg cgtggaccgc cggataattt tccttcagca ggtcgtcacc cgtgacgacg
     1321 cggatcgccg cgccgtgggt ttcggcgatg tcgcgcgccg ccgcctcgat ctgcgccggg
     1381 cccatgtcgt tggtcggcgt attgaccaga tcgcgcgccc gcgcgtgcgc cgcgacctcg
     1441 gccgccaccg ccgcgcgcgt ggccgcgtcc agctcgctgt cgtccagcac ggcctcgaca
     1501 ggcttgggcg cggccttgta gcgcgtgaag gcgtaggcgc cgaagcccca ggcggcgtgg
     1561 atcgccgcgc ggtccagtcc ctgatcggcc tccagccgcc atctgcccct gggcagttgc
     1621 ggcggcaggg cgcggaagct catcggatta tagttgcggc gcgcgcccag tccgaaccac
     1681 gcggtcaggc ccgacttgcc cggcagcgcg atcaggctgc ccgccttgcc gctaaaacct
     1741 ttcaggcgca ggaaatcggc ctcggcggcc aggccgacga cggattcggc ctcctcctcg
     1801 aagacggcga tcaggcggtt cgcggcgggg gcgggggtct tgagcggctt gggcatgggg
     1861 cggtccgtca tcgttaacga aggttaagcg cattaactct atcttcatga gattaaccga
     1921 aagcttaaaa tcggaactgc ggagagtcac gccgtgaatc gccattttcg tcttgccgtc
     1981 cgcgccgcct gttgcgcgct ggccgccggc ggccttgcgg ccggtccggc ctcggcgggc
     2041 ctgttcggca agacgcagga cgcgcccgcc gtcgccccgg cggtcagcgg cccgacccag
     2101 accagcgttt cgaccaacct gcccgtcatg gccggaaccg gcggcgccga cgacggtttc
     2161 cacaaggcgt cgaaggacga gatcgccgcc gcgctgcgcg ccgatccgct ggcgcaggcc
     2221 gccttcttca ccaaccagtt cgaccacgcg ccggacgacc cccagatggg actttacctg
     2281 tccaacgccc tgcgcgcgct gggccgttac gatcaggccg ccgacatcgc ccatcgcgtg
     2341 ctgctgttcg cgcccgacaa tgtcgatctg ctgctggccg ccgcgcgcgc ccatatcgcg
     2401 ggcgacgacg cctttctggc cgtcgcgccg ttgcagcacg tgatcgagct gcggccgaag
     2461 gactggaccg cccattcgct gctcggcgtc gcctatcagc agatcaagcg gcccgacgac
     2521 gcgcagggcg aatgggcgac ggcgctgaaa ctgtcgccgg acaatcccga cgtgctgacc
     2581 aatatcgcca tggcgaaggc ggcgggcggc gatctggccg gggccgagac gctgttgcgc
     2641 gacgccgccg cgcagaagga cgccacgacg caggtgcggc agaatctggc gctggtgctg
     2701 ggcctgcaag gcaagctggc cgaagccgag cgcctgctgc gccaggacct gccgccgcag
     2761 caggccgacg ccgacctcgc ctggctgcaa caggccgcgg cggcgcgcaa ccatgcgccc
     2821 gccgccgcgc ccgccgccgt cgaatccccc acccgctcct gggcctcggt tcaggcagcc
     2881 gggggatagg cccgcctacg cgctccatca cattttgaat cccgcgccca tttcgactcc
     2941 gcgactcgcg cgcgcggtgc tatggctggc tgcgcggtca ggcgcggcga tgcgccgcag
     3001 ccgcgcgggg tggaattatc cccaaaaaca acataccaca tcttgttatt aagctggcct
     3061 taaccacaat acgtggtgaa tatatgggca ggctttcgcg ttcagttcga ttcgggattc
     3121 accatgagca gctccatcct ccttgccgat tctcaggccg gtttgctgaa ggcgtcgcac
     3181 gcctataagc cgttccgtta tccgtgggcg ttcgagttct ggcgcaagca gcagcaggtg
     3241 cactggattc ccgaagaggt gccgctgggc gaggactgca aggactgggc ggccaagctc
     3301 aacgacggcg agcgcaattt gctgacgcag atcttccgct tcttcaccca gtcggacatc
     3361 gaggtgcagg acaattacct cgaacgctat tcgcgcgtgt tcaagccgac cgaagtgaag
     3421 atgatgctgt cctccttcgc caatatggag acgatccata tcgcggccta cgcgctgctg
     3481 ctggagacga tcggcatgcc ggagtcggag ttcggcgcgt tcatggacta tcaggccatg
     3541 cgcgacaagc acgactacat gcaggatttc ggcgtcgaca ccgaggcgga cgtgtgccgc
     3601 acgctggcca tgttcggcgg cttcaccgag gggctgcaac tgttcgcctc gttcgccatg
     3661 ctgatgaact tcccgcgctt caacaagatg aagggcatgg gccagatcgt gtcgtggtcg
     3721 gtgcgcgacg aatcgctgca ctgcgagggc atcatcaagc tgtaccacgc cttcagccgc
     3781 gaaaccggcg cggtgaccag gtccgtcgcc gacgacatca tcgactgctg caagaccgtg
     3841 gtgacgatgg aggacaagtt catcgacctg gccttcgaga tggggccggt gcagggcatg
     3901 acggccgagg acatcaaggc ctatatccgc tacatcgccg actggcgctt aaaacagctc
     3961 gaactgccgc aggtgttcga cgcgccgaag gaaaacccgc tgccgtggtt gcaggtgctg
     4021 ctgtccggcg tcgagcacgc caacttcttc gaggcgcgcg ccaccgaata ctccaaggcc
     4081 gcgaccaaag gccaatggac cggcgacgcc ggcgtctggg gcgccttcga ccagttgatg
     4141 aagaagcgcg cggaaggcat gaaggccaac gcctaggcgc cgtcgccata atgaaaaaag
     4201 ccgctccgtc cggggcggct ttcgcgcata aaaaagacgc cgctacggga ggggactacg
     4261 tagcggcgtc agacatgact ctcagggagg aaattctgtc gaccggaagg gtccgctgga
     4321 ttccaggggg gctggggggc tgttcaggga ggaacccgag ccgaccgccc cggccgacgt
     4381 tcagatcatc gcaagccaac gcggcgcgcg cggggcggaa ataaggtctt atcgcggcgg
     4441 aaagctgaac cggggataaa tggtgacggt ttcgttataa aggaggcatg cgtaagggct
     4501 tgcgcaagcc gggcggctgg ggcctaatcg gcgtatgatg gcggagtcgc gatgcgcatg
     4561 agcatacagg acgtgggcgc gcaggccgcg cgcggaccgg tgtttttcga gcgcgcggag
     4621 ttgaacctga tcctgcggct gtacggccag caggtcgctt cgggcgaatg gcgcgactac
     4681 gccatcgacc agtttcccga cgcggtcagc ttcaacatct accgccgttc gtccgaggcg
     4741 ccgctgtacc gcatcgaaaa gcgtccggcc ctcgcccgca aacagggcgc cttcgcggtg
     4801 ttcaatcagg cggggcttat cctgaaacgc ggccgcgaac tggggccggt gctgaacgtg
     4861 ctggacaagc gcaggttcga cgtcctgtaa cggggtctgg ggtcgattga ccccagcctt
     4921 ttccttcaag aaaaaagccc cgcctgaagg cgaggctttt ttcttgaaac agaagacgcg
     4981 gggtcaatcg accccgcacc ccattacagg atcgactgac ccgtcttggc ccaatccgcc
     5041 agaaacgtct ccagcccctt gtcggtcagc gggtgcttga acatgtcctt aaacgcctgc
     5101 ggcggcaggg tggcgcagtc cgcgcccgcc agagccgcct gcgcgatatg cgccgtgtta
     5161 cgcaaggacg ccgccagaat ctcggtgtcg aaatcgtaat tgtcatagat ctgacggatg
     5221 tcgtggatca actgcatccc gtccgcgccc tgatcgtcga gacggcccac gaacggcgac
     5281 acataggtcg cgcccgcctt ggccgccagc agcgcctgat tgaccgagaa gcacagggtc
     5341 acattggtca tcagaccttg cgccgaaaat tcccgcgtcg ccttcaaacc ctcgatggtc
     5401 agcggaacct tgatcaccac gttcgaggcg atcttggcca gcttctcgcc ttccctgatc
     5461 atcgtcggcg cgtcggtcgc ggcgacttcc gccgagatcg gaccgtcgat cagctcgcag
     5521 atctccttga tcacctcgaa catgttgcgg ccggacttgg cgatgatggt cggattggtg
     5581 gtcacgccgt cgatcaggcc ggtttctttc agttcggcca ggactttcgt gtcggcggta
     5641 tcggcgaaga gcagcatgtg acggcttcct ttaattggca attccggctt aacttgtcag
     5701 gaataggttt tgagattatg gggaagccct taaagcctcc gtcccaaaac agcaagtccc
     5761 cgttcatgac cacctcctca tccgcgccca gcgtcggctt cgtctccctc ggctgtccca
     5821 aggcgctggt cgattccgaa cgcatcctga cgcggctgcg cggcgaaggc tatgcgacct
     5881 cggcctccta cggcggggcc gacgtggtgg tggtcaacac ctgcggcttc ctcaattccg
     5941 ccaaggagga aagccttgcc gccatcggcg aggcgctcaa cgaaaacggc aaggtgatcg
     6001 tcaccggctg catgggcggc gaagcggacc tgatccgcca gcgcttccca gaagtcgccg
     6061 ccatcaccgg cgcgcatcag tacgacgcgg tcatggacgc ggtgcacaac atcgtcccgc
     6121 cgaagcccga tccgttccgt ccgctgatcc cggaaagcga tccgggcgtg catctgacgc
     6181 cgaagcatta cgcctacctg aaaatctccg aaggctgcga ccatcgctgc accttctgca
     6241 tcatcccgtc cttgcgcggc gacctcgcct cgcgtccggt cgccgaagtg ctgcgcgaag
     6301 ccgaaaagct ggcgcaggtg ggcgtgaagg aactgctggt cgtgtcgcag gacacctccg
     6361 cctacggcct cgacatcaaa tacgcgcaga gcgaatggcg cggccggaac tgggacgctt
     6421 cgttcgagga tttgtcgcgc ggccttggcg aactcggcgt ctgggtgcgg atgcactatg
     6481 tctatccgta cccgcatgtg aacgcggtca taccgctgat ggccgagggc aagatcctgc
     6541 cctatctcga catcccgttc cagcacgcca gcccgaagat tctcaagctc atgaagcgtc
     6601 ctggcaatca ggacaggacg ctgcaacgca tcgaatcgtg gcgcgaaatc tgccccgacc
     6661 tcacgctgcg ctcgaccttc gtggtcggct ttcccggcga gacggaggcc gatttcaacc
     6721 tgctgctcga ctggctggag gtggcgcaac tcgaccgcgt cggcgccttc gcctatgaga
     6781 acgtcaccgg cgcggcggcg caaggattgc ccgaccatgt gccggaagag gtcaagcagg
     6841 accgcctcgc ccgcttcatg gccgtcgcct cgcgcatttc ggcgcagaag ctgcaaaaca
     6901 aggtcggccg cgtcgaggac tgccttatcg acgacctgcg caacgacggc acggccatcg
     6961 cccgcaccag gggcgacgcg cccgaaatcg acggccatat cttcctgaaa ggctttaccg
     7021 gcctgaaacc gggcgatctc gtcaaggcca aggtcacccg cgccgacgcc tacgatctgt
     7081 ggggcgaacc ggaagggctg atcaagctca accgtgtgcc gggacagctt tccggcgcgg
     7141 cgcaaaagcg ccggatgcac acgctgatct cgcggattta gagcgtttcc cccaaaagtg
     7201 gttccacttt tgggggaaac gctcttaagc cacccgcacc agccgccttt gcgtgcgttc
     7261 gcccaggtcg gcgaaccatt gcgcggggtc gagcgcgacc tcgtcgttga gcgccagcga
     7321 ttcccacggg atgcgcggcg cggggcgttc ggaggcggcg ataatggcgc gcagttccgc
     7381 cgccgaggcc acgccgacga tggcggtcga ggcgccttcg atgttgagcg cgtaggacag
     7441 ggccgcgtgc agcggatcgg cgccgcattc ggccatgatg cg
//
LOCUS       DASVLL010000257         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_284456,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000257 DASVLL010000000
VERSION     DASVLL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284456"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1006)
                     /gene="argB"
                     /locus_tag="VF402_04270"
     CDS             complement(71..1006)
                     /gene="argB"
                     /locus_tag="VF402_04270"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7799541.1"
                     /translation="MIIQTETDALEEKGWATARQLAEALPYIQIYDREIVTIKYGGHA
                     MGEAETARVFASDIVLLKLLGIHPVVVHGGGPQISSMLNRAGVKSTFIDGLRVTDEAT
                     MEIAEMVLSGAVNKEIANWITRAGAEADVRGVGLSGKDAGLITVEKATRTRKDPDSMI
                     EQVVDLGFVGEPKKVDDKLIRTLIDDPNHDYVPVIAPIGVSEDGHTYNINADTVAGAV
                     AGKLNAKRMLLLTDIAGVLDADKNLIRQMSVSEARGLIASGVANGGMIPKLETAIMAI
                     ESGVEAVVILDGRRPHAMLVELFTEHGAGTLITAE"
     gene            complement(1069..2655)
                     /locus_tag="VF402_04275"
     CDS             complement(1069..2655)
                     /locus_tag="VF402_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7799542.1"
                     /translation="MSSASTTADNAPPADAGPAPLTGTALGVTAIALALGTFMQVLDS
                     TIANVSVPTIAGNLGVSTSQGTWVITSFAVANGISVPLTGWLMGRYGVVKVFIASVLM
                     FTVASFLCGIAWNIDVLILFRVLQGAVSGPMIPGSQALLMMIFPPNKRGTALAIWSMT
                     TLVAPICGPILGGYISDNISWPWIFFINLPVGAICAFLCWRGLQGRETPTFRRQIDRT
                     GFILLVVWVGALQVMLDTGKDADWFNSPVIVAETLIAAIGFVAWVVWEMYDQHPIVGL
                     SLFRLRNFALGCVVLCIGYAIFFGNNLLMPLWLQTQVGYQAFWAGLVAAPSGMVAVVL
                     TPFMARMLNRFDARILATVSMLFFSLSFYMRSLYTPDASFFTYAAPMAVMGVAMSCFF
                     ISMVAITLHGISGPLVPAASGLSNFARIVAGSFAASLATTVWDQFETRRQTHLAEIMG
                     GPGGVSGPVQQAVEQAHHLGLTTAQGAGMVVHQVVGQAYLGGALDFFRLSGIIVLALI
                     PLIWLCEETRASGGVHAAAD"
     gene            complement(2891..>3798)
                     /locus_tag="VF402_04280"
     CDS             complement(2891..>3798)
                     /locus_tag="VF402_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7799543.1"
                     /translation="SETQAVKAGDVLATVDASDAKIAVEQARADLALAQRRVQGYYAT
                     DEALGGQVGARQADVARAEAAIAAAKSDLDNAKLELTRRQNLARSGAVSQEELTSAQN
                     AYARAQAAYATATADRDAAQAAVQAASGQRATNATQINGVDAAQNPEVLAAQARLDAA
                     QLALDRTIVRAPTDGIVSKKSIEVGQQVAVGTPLMTIVPTATAYVDANFKEVQLNKVH
                     PGQKVELYSDLYGKGVTYHGTVRGLSGGTGSAFSLIPAQNASGNWIKVVQRLPVRIDL
                     DPKELKAHPLRVGLSMTARIDTSRH"
BASE COUNT          632 a         1225 c         1312 g          629 t
ORIGIN      
        1 tatgaacaga ctaaggcggc gctgcgcgcc ttttcgtgct ttttcgtgct tttcgtggcc
       61 aaaaaccttg tcactccgcc gtaatcagcg tccccgcgcc gtgttcggtg aacagttcga
      121 ccagcatggc gtgcgggcgg cggccgtcga ggatgaccac ggcttcgacg ccggactcga
      181 tggccatgat ggcggtctcc agcttcggga tcatgccgcc gttggcgact cccgacgcga
      241 tcaggccgcg cgcctcggag acgctcatct ggcggatcag gttcttgtcc gcgtccagca
      301 cgcccgcgat gtcggtcagc agcaacatgc gcttggcgtt gagcttgccc gccaccgcgc
      361 ccgccaccgt gtcggcgttg atgttgtagg tgtggccgtc ctcggacacg ccgatcggcg
      421 cgatcaccgg cacgtagtcg tggttcggat cgtcgatcag ggtgcggatc agcttgtcgt
      481 cgaccttctt cggttcgccg acgaagccga gatcgacgac ctgctcgatc atcgaatcgg
      541 gatccttgcg ggtgcgcgtg gccttttcga ccgtgatcag gcccgcatcc ttgcccgaca
      601 ggccgacgcc gcgcacatcg gcctcggcgc ccgcgcgcgt gatccagttg gcgatctcct
      661 tgttgaccgc gccggacagc accatctcgg cgatctccat cgtcgcctcg tcggtgacgc
      721 gcagcccgtc gatgaaggtc gacttcacgc ccgcccggtt cagcatcgag gaaatctgcg
      781 gcccgccgcc gtggaccacg accggatgga tgcccagcag cttcagcagc acgatgtccg
      841 aggcgaacac cctggccgtt tcggcctcgc ccatcgcatg accgccgtac ttgatggtga
      901 cgatctcgcg gtcgtaaatc tggatgtagg gcagggcttc ggccagttgc ctggcggtgg
      961 cccagccctt ttcctcaagg gcgtcggttt cggtctgaat gatcatgtgc ggcgatcctg
     1021 tactcttgcg cgcgccttac aggatcgcgc ccgccatgtc atgtgttttt agtccgccgc
     1081 cgcgtggacg ccgcctgagg cgcgggtttc ctcgcacagc cagatcagcg ggatcagagc
     1141 cagcacgatg atgcccgaca ggcggaagaa gtcgagcgcg ccgccgagat aggcctggcc
     1201 caccacctga tgcacgacca tgcccgcgcc ctgcgcggtg gtgaggccga ggtggtgcgc
     1261 ctgctcgacc gcctgctgca cggggccgga gacgccgccg gggccgccca tgatctcggc
     1321 cagatgggtc tggcggcgcg tctcgaactg gtcccagacg gtggtggcca aagaggcggc
     1381 aaacgaaccg gccacgatgc gcgcgaagtt ggacaggccc gacgcggcgg gcaccagcgg
     1441 gccggaaatg ccgtgcaggg tgatcgccac catcgagatg aagaagcaac tcatcgccac
     1501 ccccatcacc gccatcggcg cggcgtaggt gaagaagctg gcgtcgggcg tgtagagcga
     1561 gcgcatgtag aagctgagcg agaagaacag catcgacacg gtggccagaa tccgcgcgtc
     1621 gaaacggttg agcatccgcg ccatgaacgg cgtcagcacc acggccacca tgcccgacgg
     1681 cgcggcgacc agaccggccc agaaggcctg atagccgacc tgggtttgca gccacaaagg
     1741 catcaaaagg ttgttgccga agaagatcgc atagccgatg cacagcacca cgcagcccag
     1801 cgcgaaattg cgcagccgga acagcgacag gccgacgatc ggatgctggt cgtacatttc
     1861 ccacaccacc caggcgacga agccgatggc ggcgatcagg gtttccgcca cgatgaccgg
     1921 cgaattgaac cagtcggcgt ccttgccggt gtcgagcatg acctgcaacg cgccgaccca
     1981 gacgaccagc aggatgaagc cggtgcggtc gatctggcgg cggaaggtcg gcgtctcgcg
     2041 cccttgcagc ccgcgccagc acaggaaggc gcagatcgcg cccacgggca ggttgatgaa
     2101 gaagatccac ggccacgaga tgttgtcgga gatatagccg cccaggatcg gcccgcagat
     2161 cggcgccacc agcgtggtca tcgaccagat ggccagcgcc gtgccgcgtt tgttgggcgg
     2221 gaagatcatc atcagcagcg cctgcgagcc ggggatcatc ggcccggaca ccgcgccttg
     2281 cagcacgcgg aacaggatca gcacgtcgat gttccacgcg atgccgcaca ggaaggaggc
     2341 gacggtgaac atgaggaccg aggcgatgaa caccttgacc acgccgtagc gccccatcag
     2401 ccagccggtc agcggcaccg aaatgccgtt ggcgaccgcg aacgaggtga tcacccacgt
     2461 cccctgcgag gtcgagacgc ccaggttccc ggcgatggtc ggcacggaga cgttggcgat
     2521 ggtggagtcg agcacctgca tgaaggtgcc gagcgccagc gcgatggcgg tgacgcccaa
     2581 agcggtcccg gtcagcggcg cggggcctgc gtcggcgggc ggcgcgttat cggcggtggt
     2641 ggaagctgag gacattggtg ggcctcggag ggatggtgcg gactagggcg tcatgcggaa
     2701 agacggtacg cggacttaaa ccccctctcc ccgtttacgg ggggaggacg agagccgagc
     2761 ggagcgaagg cgatcgggtg aggggcctgc gcccggtcgg cggtcggccc ctcatccggt
     2821 cttcgcttcg ctgcggccac cttctcccca tggtcatggg cagaagggaa gattcagcgt
     2881 ctgtccaaaa tcaatgcctg ctcgtgtcga tcctggcggt catggacaga ccgacgcgca
     2941 gggggtgggc tttcagttcc ttcgggtcga ggtcgatgcg caccggcagg cgctgcacga
     3001 ccttgatcca gttgccggag gcgttctgcg ccgggatcag cgagaaggcc gagccggtgc
     3061 cgcccgacag gccgcgcacc gttccgtgat aggtgacgcc cttgccgtag aggtcggaat
     3121 aaagctcgac cttctggccc ggatgcacct tgttgagctg cacttccttg aagttggcgt
     3181 cgacataggc cgtggcggtc ggcacgatgg tcatcaaagg cgtgccgacg gcgacctgct
     3241 ggccgacctc gatggacttc ttcgacacga tgccgtcggt cggggcgcgc acgatggtgc
     3301 ggtccagcgc cagttgcgcg gcgtccagcc gggcctgcgc ggccaacact tcggggtttt
     3361 gcgccgcgtc gacgccgttg atctgggtcg cgttggtggc gcgctggccg ctggcggcct
     3421 gcacggcggc ctgagcggcg tcgcggtcgg cggtggcggt ggcgtaggcg gcctgcgcgc
     3481 gggcataggc gttctgcgcc gaggtcagtt cctcctgcga cacggcgccg gacctggcga
     3541 ggttctgacg gcgggtcagc tccagtttcg cgttgtcgag gtcggatttc gcggcggcga
     3601 tggcggcctc ggcgcgggcg acgtccgcct gacgggcgcc gacctgtccg cccagcgcct
     3661 cgtcggtggc gtaatagccc tgcacgcggc gttgcgccag cgcgagatct gccctggcct
     3721 gctcgaccgc gatcttcgcg tcggaagcgt cgacggtggc cagaacgtcg cccgccttga
     3781 ccgcctgagt ctcgctga
//
LOCUS       DASVLL010000258         7511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_15073, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000258 DASVLL010000000
VERSION     DASVLL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15073"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VF402_04285"
     CDS             <1..1032
                     /locus_tag="VF402_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7799544.1"
                     /translation="DAIEAFSQSLELHSDNPEAREGRIRAYEINKQPDKALADAQIYV
                     RLNPDSSDSQQMLARVLLTAKKYDEALDAYRKAAALAPDDADLLNEFGDQLTRCNGAT
                     GAACDKQHAEALAIYDKVIAIEPTAHAYIARSQVRPPLDFDLKKKDIDAALSMSPDDD
                     YALTARASLLLAQKNYAAAVADATRVIDRPHTEYGETARKVRAYAYGYLKQYDLALAD
                     WDALIAASPDEDAYLNGRAWLRATGNLDLDKALADVDAALKSKPDTAAYLDTRGFVEL
                     RMGRLDAALNAYTAALKLVPTQADSLYGRGLVERRQGQTKAGDADLAAARKIYPDIDK
                     TFADYGMTP"
     gene            1149..1850
                     /locus_tag="VF402_04290"
     CDS             1149..1850
                     /locus_tag="VF402_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799545.1"
                     /translation="MSQATGHNDAATRHLLVVDDDDRIRTLLKEFLSRQGFRVTAAAH
                     ADAAKRMLQLLDFDLLILDVMMPGEDGFSLSKWVRANSNVPILILTARGLPDDRIMGL
                     SLGADDYLSKPFEPQELLLRIEAILRRAGVKPALPKAIHMGPFSFDLERAELSKNGAP
                     VRLTEAEAQLLKFLAERANVPVDRMDLARDSVDTTGRAVDVQVTRLRRKIEQDPKNPR
                     YLQTVRGKGYMLAPD"
     gene            1850..3238
                     /locus_tag="VF402_04295"
     CDS             1850..3238
                     /locus_tag="VF402_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7799546.1"
                     /translation="MAAPPASPPRRLPRLRLWPKFLKRRFPRSLYSRVLLMIVIPIFI
                     MQIIVLWVFFSWHYETVTQKLSEGLAGDIAWAVSSYESDPSAANLARIADRAQKNMQL
                     SISLQRDPHLPPKRHASLFLALDRTIDGALNNALDDPYWFNTTAYPNYIDIRVQTKAG
                     VLRVIALKDRAFAATGYIFLFWIVGATALLTTIAMLFIRNQVRAIERLSQAAERFGRG
                     EEDTGFKPYGATEVRAAAEAFLKMKARIQRQIEQRTTLLASVSHDLRTPLTRLKLELA
                     MAAQDESTERMKSDVSEMAYMIDEYLAFARGEAAENTDSVSVRALMEAVNDSVQRAGH
                     KLQMQPPETDLRIQIREMNVQRALVNLIMNGFHYGKHVRFSAGVEERAGRRQARLHID
                     DDGPGIPPEKREEAFKPFSRLDESRNQNIKGVGLGLAIARDIVRGHGGELTLEQSPMG
                     GLRATISLPVND"
     gene            complement(3264..3494)
                     /locus_tag="VF402_04300"
     CDS             complement(3264..3494)
                     /locus_tag="VF402_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEX7799547.1"
                     /translation="MADDDVIVKDSNGNRLSDGDSVTLIKDLKVKGSGGVTLKRGTLI
                     RNIRLTYDEAEIECNADKVKGLVLRTEFVKKA"
     gene            complement(3494..4297)
                     /gene="proC"
                     /locus_tag="VF402_04305"
     CDS             complement(3494..4297)
                     /gene="proC"
                     /locus_tag="VF402_04305"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX7799548.1"
                     /translation="MSLQKPVLLIGAGALGSSLLKGLRIAGNATPSEVMILDLKPGDE
                     ANAWAADGARLNPVPDEWRAARTLVLAVKPQGWHAVAAILHGNLHADAAIVSVMAGVT
                     TAQLKTAFAGHAVARVMPTTGVATGKGVATLFSDSEAGMDAARGLFGPVAQTVELSDE
                     SQMNSATAVSGSGPAYVYAFVRALEKAGRATGLSSSHARDLARGTLISAARLLDQTNE
                     EPDELIRKVASPGGTTEAALNVLCREGAGLDELVLAAVEAAHQRSKELG"
     gene            complement(4301..4840)
                     /locus_tag="VF402_04310"
     CDS             complement(4301..4840)
                     /locus_tag="VF402_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX7799549.1"
                     /translation="MTATQIQDGDNAFDPLDMVESILTEEGLEYERTPEGDLGFALKG
                     DWRAYEMWFSWRPEGECLQLCCSLDVESVAGRLAPERLPGLYELVCLVNQHVWFGHFE
                     VFCDKDDDNEDTFEVVFRHTLGVSLPDRAAMMSGAHMINSAAEAVDRFYPAFDFWFKG
                     AETAQAAFSACLFETQGEA"
     gene            complement(4878..5150)
                     /locus_tag="VF402_04315"
     CDS             complement(4878..5150)
                     /locus_tag="VF402_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX7799550.1"
                     /translation="MQSQNPFLDEFAKMTANAMSLAQAAGEEAKSAFRAQADRFVADM
                     DLVRREEIDALKAEIADLRAQVAALKGEAAPKGRRGGKPSPDMAQD"
     gene            5361..6230
                     /gene="lgt"
                     /locus_tag="VF402_04320"
     CDS             5361..6230
                     /gene="lgt"
                     /locus_tag="VF402_04320"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7799551.1"
                     /translation="MKLPDIDPVLLHLGPVNIHWYAIAYIAGIVCGWLYVSRLLRRED
                     LWAPRTPPITTAQLDDLILWIMLGIILGGRIGYIIAYDTSIIWEHPLQMFQIWNGGMS
                     FHGGFLGVMTASLLYCRARKLDAAHILNLGDALAAAAPIGLFFGRLANFVNGELWGRP
                     TTLPWGMDFCNKYILKQYNGVCPADPDGVPVLRHPSQLYEALGEGVILFAIMWLLIHR
                     FGKLKQPGLIMGVFVAGYGIVRILLENVRNPDAQMPDFLRHWITMGMILSIPMVLIGA
                     FLIWNSRRMARHA"
     gene            6223..7284
                     /locus_tag="VF402_04325"
     CDS             6223..7284
                     /locus_tag="VF402_04325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7799552.1"
                     /translation="MLRRRLIEQITLDGPISVADYMTRCLLDPRDGYYTRHVKLGADG
                     DFLTAPLVSQMFGEMIGVWAAEIWRGLGSPAAFTLTEIGGGDGTLMRDVLRVMARVPG
                     LSEAAQVVMIEASAPLRAQQAAIPGVRHVEALDAIDASRPVILIGNEILDCLPARQFV
                     KTGKGWHERRVGLIDGDLGFGLAEAGPDFAPPFEAPEGEIIEISTAQVRFAEQAAALL
                     RQASGAALLIDYGRDAPGTGDTLQALHRHRKTDPLAAPGEHDLTMWADFPAVAAAARA
                     GGVKAALTTQGDFLRALGIEARFEALRQHNPDKAASLQRQLSRLTAKGQMGGLFKVLS
                     LGFPEQIRLIGVEATESPL"
BASE COUNT         1269 a         2553 c         2393 g         1296 t
ORIGIN      
        1 gacgccatcg aagccttcag tcagtctctg gagttgcatt cggataatcc ggaggcgcgc
       61 gagggccgca tccgcgccta cgaaatcaac aagcagccgg acaaggcctt ggccgacgcg
      121 cagatatatg tgcggctgaa ccccgattcg tccgactcgc aacagatgtt ggcgcgcgtc
      181 ttattgacgg cgaaaaaata cgacgaggcg cttgacgcct atcgcaaggc ggcagcgctg
      241 gcgccggacg atgcggacct gctgaacgaa ttcggcgatc aactgacacg atgtaacggc
      301 gcgacgggcg cggcttgcga caagcagcac gcggaagctc tggcgatcta cgacaaggtg
      361 atcgcaatag aaccgaccgc gcacgcctat atcgcgcgct cgcaggtgcg tccgcctctc
      421 gatttcgatc tgaagaagaa ggatatcgac gccgccctgt ccatgtcgcc ggacgacgac
      481 tacgccctga ccgcgcgggc ctcgctgctg ctggcgcaga aaaattatgc ggcggcggtc
      541 gccgatgcaa cgcgcgtcat cgaccgcccg catacggagt acggcgagac agcgcgcaag
      601 gtgcgcgcct atgcctatgg atatctgaag caatacgacc tggcgctcgc cgactgggac
      661 gccttgatcg ccgcgtcacc cgatgaagac gcctatctca atggccgcgc ctggctgcgc
      721 gccacaggca atctcgatct cgacaaggcg ctggccgatg tcgacgcggc gttgaaatcg
      781 aagcccgaca cggccgccta tctcgacacg cgcggctttg tcgaactgcg catgggacgt
      841 ctggatgcgg cgctgaacgc ctacaccgcg gcgttgaagc ttgtccccac ccaggccgat
      901 tccctgtatg gacgcggcct cgtcgaacgc cgtcagggcc agacgaaagc gggcgacgcc
      961 gatctggcgg cggcgcggaa aatctatccc gacatcgaca agaccttcgc cgattacggc
     1021 atgacgcctt agttcgcagg tttttgcctg tctgcgcttt acatttcacg gcgaatagtg
     1081 atcagactgt tccggcatcc gtgttttatc cccggcgcga gaccggtcac aggacagaga
     1141 cgacaaaagt gagccaggcc acaggtcaca acgacgccgc cacgcggcat cttctggttg
     1201 tcgacgacga cgaccgcatc cgcacccttt tgaaggagtt cctgtcgcgg caggggttcc
     1261 gggtcaccgc cgccgcgcac gccgacgcgg ccaagcggat gttgcaactg ctcgacttcg
     1321 accttttgat cctcgacgtg atgatgccgg gcgaggacgg tttttcgctg tcgaagtggg
     1381 tgcgcgccaa ttccaacgtg ccgatcctga tcctgacggc gcgcggcctg ccggacgacc
     1441 gcatcatggg cctgtcgctg ggcgcggacg attacctgtc caagccgttc gagccgcagg
     1501 agctgctctt gcgcatcgag gcgatcctgc gccgcgcggg cgtgaagccg gccctgccca
     1561 aggcgatcca catggggccg ttcagcttcg atctggagcg cgccgagttg agcaagaacg
     1621 gcgcgccggt gcgcctgacc gaggccgagg cgcagttgct gaaattcctg gccgagcgcg
     1681 ccaacgtgcc ggtcgaccgc atggacctgg cgcgcgacag cgtcgacacc accgggcgcg
     1741 cggtcgacgt gcaggtcacg cgcctgcgcc gcaagatcga gcaggacccg aagaatccgc
     1801 gctacctgca aaccgtgcgc ggcaagggct atatgctggc gccggattga tggccgcgcc
     1861 gcctgcgtcc ccgccccgcc gcctgccccg gctgcggctg tggccgaaat tcctgaagcg
     1921 ccgcttcccg cgcagcctgt attcgcgcgt gctgctgatg atcgtgatcc cgatcttcat
     1981 catgcagatc atcgtgcttt gggtgttttt cagttggcac tacgagacgg tgacgcagaa
     2041 gctgtccgag gggctggcgg gcgatatcgc ctgggcggtc agcagctacg agagcgatcc
     2101 gtcggcggcc aatctggcgc gcatcgccga ccgggcgcag aagaacatgc agttgtcgat
     2161 ctcgctgcaa cgcgatccgc acctgccgcc caagcggcac gcctcgctgt tcctggcgct
     2221 ggaccgcacc atcgacggcg cgctcaacaa cgcgctggac gatccgtact ggttcaacac
     2281 cacggcctat ccgaactata tcgacatccg cgtgcagacg aaggcgggcg tgctgcgcgt
     2341 catcgcgctc aaggaccgcg ccttcgcggc cacgggctat atcttcctgt tctggatcgt
     2401 cggcgcgacg gcgcttttga ccaccatcgc catgctgttc atccgcaatc aggtgcgcgc
     2461 catcgagcgc ctgagccagg ccgccgagcg cttcgggcgc ggcgaggagg atacgggctt
     2521 caagccctac ggcgccaccg aggtgcgcgc cgccgccgag gccttcctga agatgaaggc
     2581 gcgcatccag cgccagatcg agcagcgcac cacgcttttg gcctcggtca gccacgacct
     2641 gcgcacgccg ctgacccggc tgaagctgga actggccatg gcggcgcagg acgagtcgac
     2701 cgagcgcatg aagtcggacg tgtcggaaat ggcctatatg atcgacgaat atctggcctt
     2761 cgcgcgcggc gaggcggcgg agaacaccga cagcgtgtcg gtgcgcgcgc tgatggaggc
     2821 cgtcaacgac agcgtgcagc gcgccgggca caagttgcag atgcagccgc cggaaaccga
     2881 tctgcgcatc cagatccgcg agatgaacgt gcagcgcgct ctagtcaacc tgatcatgaa
     2941 cggctttcat tacggcaagc acgtccggtt cagcgccggg gtcgaggagc gcgccgggcg
     3001 gcggcaggcg cgcctgcata tcgacgacga cggccccggc atcccgccgg aaaagcgcga
     3061 ggaggcgttc aagccgttct cgcgcctcga cgaatcgcgc aaccagaaca tcaagggcgt
     3121 cggcctcggt ctggccatcg cgcgcgacat cgtgcgcggc cacggcggcg aactgacgct
     3181 ggagcaaagc ccgatgggcg ggctgcgcgc gaccatcagc ctgccggtga acgactgacc
     3241 gcgccgtccc cggttggctc aggtcaggct tttttcacga actcggtgcg caggaccagc
     3301 cccttgacct tgtcggcgtt gcattcgatt tcggcttcat cataggtcag gcggatattg
     3361 cggatcagcg tcccccgttt cagggtcacg ccgcccgatc ccttgacctt caagtccttg
     3421 atcagggtca cgctgtcgcc gtcgctcagg cggttgccgt tgctgtcctt cacgatcacg
     3481 tcgtcgtcgg ccatcagccc agttccttcg agcgctgatg cgcggcctcg accgccgcca
     3541 gcaccagttc gtccagcccc gcgccttcgc ggcacagcac gttcagcgcc gcctcggtcg
     3601 tgccgccggg cgaggcgacc ttgcggatca gctcgtccgg ctcctcgttc gtctggtcca
     3661 gcagccgggc cgccgagatc aatgtaccac gcgccagatc gcgcgcgtgg ctggacgaca
     3721 ggccggtcgc ccgtcccgcc ttctccagcg cgcgcacgaa ggcatagaca taggccgggc
     3781 cggagcccga cacggcggtg gcggaattca tctggctttc gtcggacagt tcgaccgtct
     3841 gcgcgaccgg gccgaacagg ccgcgcgcgg cgtccatacc cgcctcgctg tccgagaaca
     3901 gggtggccac gcccttgccg gtggccacgc ccgtggtcgg catgacgcgc gcgaccgcat
     3961 ggccggcgaa ggcggtttta agctgcgccg tcgtcacgcc cgccatcacc gagacgatgg
     4021 ccgcgtccgc gtgcaggttg ccgtgcagta tggccgcgac cgcgtgccag ccctgcggct
     4081 tgacggccag cacaagggtg cgcgccgcgc gccattcgtc cgggaccgga ttgagccgcg
     4141 cgccgtcggc ggcccaggcg ttggcctcgt cgcccggctt gaggtcgagg atcatcacct
     4201 cggacggcgt cgcgttgccc gcgatgcgca ggcccttgag cagggacgac cccagcgccc
     4261 cggcgccgat cagcagaaca ggtttttgca gcgacatctt ttaagcctcc ccctgcgttt
     4321 cgaacaggca ggcgctgaac gccgcctgcg ccgtctccgc gcccttgaac cagaaatcga
     4381 aggccggata gaagcggtcg accgcctcgg cggcgctgtt gatcatgtgc gcgcccgaca
     4441 tcatcgcggc gcgatccggc aggctgacgc ccagggtgtg ccggaacacg acctcgaacg
     4501 tgtcctcgtt atcatcgtcc ttgtcgcaga aaacctcgaa atggccgaac cagacgtgct
     4561 ggttgacgag gcagaccagt tcgtaaagcc ccggcaggcg ctccggcgcc agccgcccgg
     4621 cgacgctttc gacgtccagc gagcagcaca gttgcaggca ctcgccctcc ggccgccacg
     4681 aaaaccacat ctcataggcg cgccagtcgc ctttcagcgc gaagccgaga tcgccttcgg
     4741 gcgtgcgctc gtattcgagc ccctcctcgg tcaggatgga ctcgaccatg tcgagcgggt
     4801 cgaaggcgtt gtcgccgtcc tgtatttggg ttgcggtcat aaggtctttc tggggcttgc
     4861 ttttcttagt cctgcgccta atcctgcgcc atgtccggcg acggcttgcc gccgcgacgg
     4921 cctttcggcg ccgcctcgcc tttcaaagcc gccacctgcg cgcgcaggtc ggcgatctcg
     4981 gccttcagcg cgtcgatctc ctcgcgccgc accagatcca tatcggccac gaagcggtcg
     5041 gcctgcgcgc ggaaggccga tttggcctcc tcgcccgcgg cctgagccaa actcatggcg
     5101 ttggcggtca tcttggcgaa ttcgtcaagg aacggatttt ggctttgcat cagaactgaa
     5161 ctccgaaagg gacgcgccat catacagggg cggttcgcgc gcttcaatgc tgcggcgcat
     5221 cccggcgaat ccttccccgg cattattcat gattctggcc cgctcgcctc tgttttgtcg
     5281 cgactttgca gcatgaactt gttaaacacc gccgcaaccg caacccttgt caaagaggcg
     5341 ccgccttgcc gatcctgagc ctgaaactgc cggacatcga tccggttctg ctccatctcg
     5401 gtcctgtcaa tatccactgg tacgccatcg cctatatcgc gggcatcgtc tgcggctggc
     5461 tgtacgtcag ccgccttttg cgccgcgaag acctgtgggc gccgcgcacg ccgccgatca
     5521 cgacggcgca gctcgacgac ctgattttgt ggatcatgct gggcatcatc ctcggcggac
     5581 gcatcggcta catcatcgcc tacgacacct cgatcatctg ggagcatccg ttgcagatgt
     5641 tccagatctg gaacggcggc atgtcgttcc acggcggctt tctcggcgtg atgacggcca
     5701 gcctgctcta ttgccgcgcc agaaagctcg acgccgccca catcctcaat ctgggcgacg
     5761 cgctggccgc cgccgcgccg atcggcctgt tcttcgggcg gctggccaat ttcgtcaatg
     5821 gcgagctgtg gggccgtccg acgacgctgc cgtggggcat ggacttctgc aacaagtaca
     5881 tcctcaagca atataacggc gtctgccccg ccgacccgga cggcgttccg gtgctgcgcc
     5941 accccagcca gctttacgag gcgctgggcg aaggcgtgat cctgttcgcg atcatgtggc
     6001 ttttgatcca ccgtttcggc aagctgaaac agccgggcct gatcatgggc gtgttcgtcg
     6061 ccggttacgg catcgtgcgc atcctcctgg aaaacgtgcg caatcccgac gcgcagatgc
     6121 cggacttcct caggcactgg atcacgatgg gcatgatcct gtcgatcccg atggtgctga
     6181 tcggggcctt cctgatctgg aattcgcgcc gcatggcccg acatgcttag gcgcaggctg
     6241 atcgagcaga tcacgctcga cggaccgatc agcgtggccg actacatgac gcgctgcctg
     6301 ctcgacccgc gggacggcta ttacacccgg cacgtgaagc tgggcgccga cggcgatttc
     6361 ctcaccgcgc cgctggtctc gcagatgttc ggcgagatga tcggcgtctg ggcagcggaa
     6421 atctggcgcg gcctgggatc gcccgccgcc ttcaccctga ccgagatcgg cggcggcgac
     6481 gggacgctga tgcgcgacgt gctgcgcgtg atggccaggg ttccgggcct gagcgaggcc
     6541 gctcaggtcg tgatgatcga ggcgtcggcg cccctgcgcg cccagcaggc cgccattccc
     6601 ggcgtccgcc atgtcgaagc gctggacgcc atcgacgctt caaggcccgt catcctgatc
     6661 ggcaacgaga ttctcgactg cctgcccgcg cgccagttcg tcaagaccgg caagggctgg
     6721 cacgagcgcc gcgtcggcct gatcgacggc gatctcggct tcggtctggc cgaggccggt
     6781 cccgacttcg cgccgccctt cgaggcgccg gaaggcgaaa ttatcgaaat ctccaccgcg
     6841 caggtccgct tcgccgagca ggccgccgcc ctgctgcgtc aggcaagcgg cgcggcgctg
     6901 ctgatcgact acggccgcga cgcgcccgga accggcgaca cgcttcaggc cctgcatcgc
     6961 caccgaaaga ccgacccgct ggccgcgccg ggcgaacacg acctgaccat gtgggccgac
     7021 ttccccgccg tcgcggcggc ggcgcgcgcg ggcggcgtca aggcggcgct gacgacgcaa
     7081 ggcgatttcc tgcgcgcgct ggggatcgag gcgcgcttcg aggcgttgcg ccagcataac
     7141 ccggacaagg ccgcatccct gcaacgccag ctttcgcgcc tgaccgccaa aggccagatg
     7201 ggcggcctgt tcaaggttct gagcctcggt tttccagagc aaattcgcct gattggggtt
     7261 gaagccacgg aatcgcccct ttagattcgc ataactcgac ccgctgtccc tcagggggat
     7321 cgcacgcaca acagacgccc ttttttcctc ccctgtgaaa cgggggacgc agcgcggcca
     7381 tgaaatggcc gtgacggagg gggcggcgca ggcggaggcc ccctccgtcg gcttcgccgc
     7441 cacctccccc gtttcacagg ggaggaaaaa gggcgtctgt tgtgcgtgcg atccccctga
     7501 gggacagcgg g
//
LOCUS       DASVLL010000259         4553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_500064,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000259 DASVLL010000000
VERSION     DASVLL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500064"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1461
                     /locus_tag="VF402_04330"
     CDS             256..1461
                     /locus_tag="VF402_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7799553.1"
                     /translation="MSLSNYRLLRTLSRLASLGRKPGDLPLPDPSDPASFKVLLHRPQ
                     VTLDTEGLKRFYSGKRILVTGAGGSIGSEIVKQALALGAAHVTLVDHSELALYEIDRA
                     VREGFEGRRAHPVLCSVRRRERVAAVMKAARPDVVFHAAALRHVPMVELNPSESVLTN
                     VLGTQYVIDAAKAAGVGQLVLISSDKAVAPSSLMGATKRLAEHLIRTQITDEHQACVV
                     RFGNVLGSTGSVVPLFKSQIERGGPVTLTDKDAERYFMTIFEAVQLVLTAAMHNAETP
                     HHKSGLYILEMGQPVRILDLAHRLIRLYGMKPDKDVKVVFTGLREGEKVTEALVDDNE
                     MRQPLLPGIFEVANQAHVAPLADEIMHTLYALAQEGHDEPARTMVFNLVNSLRDPAAP
                     PLARVVNAE"
     gene            complement(1506..1631)
                     /gene="cydX"
                     /locus_tag="VF402_04335"
     CDS             complement(1506..1631)
                     /gene="cydX"
                     /locus_tag="VF402_04335"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEX7799554.1"
                     /translation="MWYFSWILGVGLAVSVGILNGIWYEFHTTDDPDAEDPHREG"
     gene            complement(1644..2804)
                     /gene="cydB"
                     /locus_tag="VF402_04340"
     CDS             complement(1644..2804)
                     /gene="cydB"
                     /locus_tag="VF402_04340"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX7799555.1"
                     /translation="MELPLDYMTLRVIWWLLVGVLLTGFAVTDGFDMGVAALLPFVAK
                     SDRERRAVINTVGATWEGNQVWFVLGGGAIFAAWPFVYGASFSGFYLAMFLVLMALIL
                     RPVGFKYRSKRPGAAWRRNWDWALFAGGLIPALVFGVAIGNVLQGAPFSFDSDLRMTY
                     HDHLLGGLARLFSPFTLVCGLASVSMLVLQGASWLGVKLEKGETRDRAMRYGMIAAVA
                     LIVLFAVGHVFVRFTGLGYAVVGQVDPNGVSNPTRAEVVRQAGGWLRNYGQYPWMWIA
                     PAIGFLGPVLALIALKTRRDLLAFLASSLTIFGVIGTVGFSMFPIILPSSVDKHSSLL
                     VWTASSSPMTLFIMLLVTAVLLPLVLYYTAWVYKVLFGRIGVGDVAANPDLY"
     gene            complement(2816..4390)
                     /locus_tag="VF402_04345"
     CDS             complement(2816..4390)
                     /locus_tag="VF402_04345"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX7799556.1"
                     /translation="MDLSTVELSRLQFALTAMYHFLFVPLTLGLSFMLVIMESVYVLT
                     RKEIWRTITRFWGMLFGINFAIGVATGLTMEFEFGMNWSYYSHYVGDIFGAPLAIEGL
                     MAFFLEATFVGLMFFGWDRLSRPAHLLVTFLVALGTNLSALWILIANGWMQHPTGAVF
                     NPQTMRMEVSNFMDVVFNPVAQAKFVHTVSAGYVLAAVFVLGISAIYLLRGRYVSVAK
                     RSMAVAAAFGLAASLSVVVLGDESGYTLTDNQKMKLAALEAMWHTEKAPAGITIFGLP
                     DLKTHTTRAEIKLPYVLGLIATRSLDKPVVGILELVDIANHRIHNGVLAYGAVQALKA
                     NPNDLGARAIFEATKADLGYGLLLKRYVADPRTASEAVIARAAADTIPNVPLMFWAFR
                     VMAGLGFAFIALFVAAFFMVTFNRHRNKPFLILCILALPFPWIAIEGGWMLAEIGRQP
                     WAVDGVLPTFMGASSLTAPQIWLTIIGFTLMYAVMAVIEIKLMVRTIGKGPYEAKFGR
                     LHDETDAPALEAAPAE"
BASE COUNT          921 a         1475 c         1446 g          711 t
ORIGIN      
        1 atgcccggcg gacagcgccg ccgcgaagat ttcgtcgcgc aaagcgaaca aaccgcccgc
       61 cgcgccgtcg taacggcggc tgcggctttg cgtcagatcc gccatgcgtc cgaaaaataa
      121 aatttcaatc atgcgaccct aaacctgtgg gcgaataaag cgtggcaatt ttacacgccc
      181 gcttgtatta aaggcttgcc aaaagtcata gatcaagctt gataaccgga aaagagtcta
      241 tctgttgaga cgtcaatgag tctgtcgaat tatcgccttc tgcgtacttt aagcagactt
      301 gcgtctctgg gccggaaacc gggcgacctg ccgctgcccg atccgagcga tccggcttcg
      361 ttcaaggtat tgctgcaccg ccctcaggtg accctggaca ccgagggcct gaaacgcttt
      421 tacagcggca agcgcatact ggtcaccggc gcgggcggct ccatcggctc ggagatcgtc
      481 aagcaggcgc tggcgctcgg cgccgcgcac gtcacgctgg tcgatcactc cgaactggcg
      541 ctgtacgaga tcgaccgcgc ggtgcgcgaa ggcttcgagg gccgccgcgc ccatccggtt
      601 ctgtgcagcg tccggcggcg cgagcgcgtc gcggcggtca tgaaggccgc ccggccggac
      661 gtggtgttcc acgccgccgc gctcaggcac gtgccgatgg tcgagctgaa cccgtccgaa
      721 agcgtgctga ccaacgtgct gggcacgcaa tatgtgatcg acgcggccaa ggcggcgggc
      781 gtggggcaac tggtgctgat ctcgtccgac aaggcggtgg cgccgtcctc gctgatgggc
      841 gcgaccaagc ggctggccga acacctgatc cgcacccaga tcaccgacga gcatcaggcg
      901 tgcgtcgtgc gcttcggcaa cgtgctgggc tcgaccggct cggtcgtgcc cctgttcaag
      961 tcgcagatcg agcgcggcgg tcccgtcacc ctgacggaca aggacgccga gcgctatttc
     1021 atgaccattt tcgaggccgt gcaactggtg ctgaccgccg ccatgcacaa tgccgagacg
     1081 ccgcatcaca agtcgggcct gtacattctg gaaatgggcc agccggtgcg gattctcgac
     1141 ctggcgcacc gcctgatccg gctgtacggc atgaagccgg acaaggacgt caaggtcgtg
     1201 ttcaccggcc tgcgcgaagg cgagaaggtc accgaggcgc tggtcgacga caacgaaatg
     1261 cgccagcccc tgctgcccgg cattttcgag gtcgccaatc aggcccatgt cgcgccgctg
     1321 gccgacgaga tcatgcacac cctgtacgcg ctggcgcagg aaggccatga cgaaccggcg
     1381 cggaccatgg tgttcaacct cgtcaattcg ctgcgcgacc ccgccgcgcc gccgctggcg
     1441 cgggtcgtca acgcggaata gcaaaaacgc cggcctgtcg gggccggcgt ttttcttacg
     1501 ggcgctcacc cttccctgtg cgggtcttcg gcgtccgggt cgtccgtggt gtggaactcg
     1561 taccagatgc cgttgaggat gccgacggaa acggccaggc cgacgcccaa tatccatgaa
     1621 aaataccaca tggcttttgc tccttaatac agatcgggat tggccgcgac gtcgccgacg
     1681 ccgatccgcc cgaacaggac cttgtacacc cacgccgtat agtacagcac cagcggcagc
     1741 aacaccgccg tgaccagcag catgatgaac agggtcatgg gcgaggacga ggcggtccag
     1801 accagcaggc tggagtgctt atcgacgctg gacggcagga tgatcgggaa catcgagaag
     1861 ccgaccgtgc cgatcacgcc gaagatcgtc agggacgagg ccagaaaggc cagcaggtcg
     1921 cggcgcgtct tcaaggcgat cagggccaga accgggccga ggaagccgat ggcgggcgcg
     1981 atccacatcc acggatactg accgtaattg cgcagccagc cgcccgcctg acgcacgacc
     2041 tcggcgcggg tcgggttgga gacgccgttc ggatcgacct gaccgacgac cgcgtaaccg
     2101 aggccggtga agcgcacgaa gacgtggccg acggcgaaca gcacgatcag ggcgacggcg
     2161 gcgatcatgc cgtagcgcat cgcccggtcg cgcgtctcgc ccttctccag cttgacgccc
     2221 agccagctcg ccccttgcag gaccagcatg gacacggagg ccaggccgca gaccagcgtg
     2281 aacgggctga acagacgcgc gaggccgccc agcaggtgat cgtgataggt catgcgcagg
     2341 tcggaatcga agctgaacgg cgcgccttgc agcacgttgc cgatggccac gccgaacacc
     2401 agcgccggga tcagcccgcc cgcgaacaag gcccagtccc agttgcgccg ccacgccgcg
     2461 ccggggcgct tggagcggta cttgaagccg accggccgca ggatcagcgc catcagcacc
     2521 agaaacatgg cgaggtagaa gccggagaag ctggcaccgt aaacgaacgg ccaggccgcg
     2581 aagatcgcgc cgccgccgag gacgaaccag acctgattgc cctcccatgt cgcgcccacc
     2641 gtgttgatga cggcgcggcg ctcgcgatcg cttttcgcca cgaagggcag cagcgccgcc
     2701 acgcccatat cgaagccgtc ggtgacggcg aatccggtca gcagcacgcc gaccagcagc
     2761 caccagatga cgcgcaaagt catgtagtcg agaggcagtt ccatcgtcgc gctccttatt
     2821 cagcgggcgc cgcttcgaga gcgggcgcat cggtttcgtc gtgcaggcgg ccgaatttgg
     2881 cctcgtacgg tcccttgccg atggtgcgga ccatcagctt gatctcgatc accgccatga
     2941 cggcgtacat cagggtgaag ccgataatgg tcagccagat ctgcggcgcc gtcaggctgg
     3001 aggcgcccat gaaggtcggc agcacgccgt ccaccgccca cggctggcgc ccgatctcgg
     3061 ccagcatcca gccgccttcg atggcgatcc acgggaacgg cagggcaagg atgcacagga
     3121 tcaggaacgg cttgttgcga tgccggttga aggtcaccat gaagaaggcg gcgacgaaca
     3181 gggcgatgaa ggcgaagccc agcccggcca tgacgcggaa cgcccagaac atcagcggca
     3241 cgttgggaat ggtgtccgcc gccgccctgg cgatcaccgc ctcgctggcc gtgcgcggat
     3301 cggcgacgta ccttttcagc agcaggccgt agccgaggtc agccttggtc gcctcgaaga
     3361 tggcgcgcgc gcccagatcg ttcggattgg ccttgagcgc ctgcaccgcg ccataggcca
     3421 gcacgccgtt atggatgcgg tgattggcga tgtcgaccag ttcgagaatg ccgaccaccg
     3481 gcttgtcgag gctgcgcgtc gcgatcaggc ccagcacgta gggcagcttg atctcggccc
     3541 tggtggtatg ggtcttgaga tcgggcaggc cgaagatggt gatgcccgcc ggggcctttt
     3601 cggtgtgcca catggcctcc agcgcggcca gcttcatctt ctggttatcg gtcagggtgt
     3661 agcccgactc gtcgcccagc accacgaccg acagggacgc ggccagaccg aaggccgccg
     3721 ccaccgccat cgagcgcttg gccacgctga catacctgcc gcgcagcaga tagatggccg
     3781 atatgcccag cacgaacacc gccgccagca cgtaaccggc gctgaccgta tgcacgaact
     3841 tggcctgcgc caccgggttg aacaccacgt ccatgaagtt cgacacctcc atgcgcatgg
     3901 tctgcgggtt gaagaccgcg ccggtcggat gctgcatcca gccgttggcg atcaggatcc
     3961 acagcgccga caggttggtg cccagcgcga ccagaaaggt caccagaagg tgcgcgggcc
     4021 gcgacagtct gtcccagccg aagaacatca gcccgacgaa ggtcgcttcg aggaagaagg
     4081 ccatcagccc ctcgatcgcc agcggcgcgc cgaaaatgtc gccgacatag tgcgaataat
     4141 aggaccagtt catgccgaac tcgaactcca tcgtcaggcc ggtggcgacc ccgatggcga
     4201 agttgatgcc gaacagcatg ccccagaagc gcgtgatggt gcgccagatc tccttgcgcg
     4261 tcagcacata gacgctttcc atgatcacca gcatgaagct gaggcccagc gtcagcggca
     4321 cgaacaggaa gtgatacatc gccgtcaggg cgaattgcag ccgcgacagt tctacggtac
     4381 tgaggtccat ccgaactctc ccctaaacac taatgcccgg tacgcacgta ttgcgcgaat
     4441 tagacacgat aacgcagttt tttcaatgcc gacgtcacag atagcgagac agcggcggta
     4501 aaaacccata caaagcgtaa ggcttaattt cgcagcgcac aattaccgcc gat
//
LOCUS       DASVLL010000260         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_390347,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000260 DASVLL010000000
VERSION     DASVLL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390347"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF402_04350"
     CDS             <1..531
                     /locus_tag="VF402_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX7799557.1"
                     /translation="DQAVLARIAPGLAWLSLALSSLTALERLFERDYEDGLFDLLRQG
                     PLPLEAVALIKVAAQWLGTGLILAVMTPVVMIVLGAPARLALNGLIAALLGSLSFALI
                     GGVGAALALGARKGGALMALLVLPFYVPPVIFGAALMTAGPAHMQALAFLGAYALFAL
                     ALGPVAMGAALRHGIS"
     gene            complement(543..839)
                     /locus_tag="VF402_04355"
     CDS             complement(543..839)
                     /locus_tag="VF402_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799558.1"
                     /translation="MLATLVVPVALAGVTFVLYRCGVREGCRWAAWLAALVMAAGSFT
                     VLHVFERGLTAGVLLESLVLVMAGGGLIARQRLGRQDAGRRSHFRVHRLKRGLF"
     gene            982..1683
                     /gene="ccmC"
                     /locus_tag="VF402_04360"
     CDS             982..1683
                     /gene="ccmC"
                     /locus_tag="VF402_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX7799559.1"
                     /translation="MFSFLANPERFSRVTRPLRPWLAGLCALCLLWGLYLAFASPEDY
                     QQGDTVRIMYVHVPSAWMGLLIYALMGVASFFGLIFRHALADAAAKAMAPVGAVFTGL
                     ALLTGALWGWPMWGTPWQWDGRMTSELVLFLFYVAYIALHASIEDETRAARMSAILAL
                     VGLVNLPIVHYSVLWWNGLHQGNRIETTGAILWPLFVMTAAWHLLFAWLFLVRLDAEI
                     LERRYRALRARLNYQ"
     gene            1691..1855
                     /gene="ccmD"
                     /locus_tag="VF402_04365"
     CDS             1691..1855
                     /gene="ccmD"
                     /locus_tag="VF402_04365"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX7799560.1"
                     /translation="MTLDLDMGKYALFVWGSYGATFAALTALVVVSLRAQAARRRRLE
                     ALRQAAEDVT"
     gene            1852..2373
                     /locus_tag="VF402_04370"
     CDS             1852..2373
                     /locus_tag="VF402_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX7799561.1"
                     /translation="MKRLIYALPLILFAGLLAVLAWYNFHKKASYEPRAMVGKAVPEI
                     VLNDPQGGPPSDLKSLLAGRTRPVLVNVFASWCVPCMAENPQLMALKAKGVVIVGVAW
                     KDKPQDALNFLAQHDNPYVKTLSDPNGDMALALGISGVPETYIVAPDGTITDKITGPI
                     VPDTLDSVLKEAR"
BASE COUNT          357 a          791 c          815 g          430 t
ORIGIN      
        1 gatcaggcgg ttctggcccg catcgcgccg ggtctggcct ggctcagcct cgcgctcagc
       61 agcctgacgg cgctggagcg cctgttcgag cgcgattacg aggacggcct gttcgacctg
      121 ctgcggcaag gccccctgcc gttggaggcg gtggcgctga tcaaggtcgc ggcgcaatgg
      181 ctggggacgg ggctgattct ggccgtgatg acgcccgtgg tgatgatcgt gctgggcgcg
      241 cccgcgcgtc tggcgctgaa cggcctgatc gccgccctgc tcggctcgct cagcttcgcg
      301 ctgatcggcg gcgtcggggc cgctctggcg ctgggggcgc gcaagggcgg cgcgctgatg
      361 gcgctgctgg tgctgccgtt ctacgttccg ccggtgattt tcggcgccgc gctgatgacc
      421 gccggacccg cgcacatgca ggctttggcc tttctcggcg cctatgcgct gttcgcgctg
      481 gcgctcggcc ccgtcgccat gggcgcggcg ctccggcacg gtatttcata atatctggaa
      541 attcagaaca gaccgcgttt caggcgatgc acgcgaaaat gcgaacgccg cccggcgtcc
      601 tgacgcccga ggcgctggcg cgcgatcagc ccgccccccg ccatgaccag caccagactt
      661 tccagcagca cgcccgcggt caggccgcgc tcgaacacgt gcagcaccgt gaaactgccc
      721 gccgccatca ccagcgccgc cagccacgcc gcccagcggc agccttcacg cacgccgcag
      781 cggtacagaa cgaaggtgac gcctgccagc gccaccggaa caacaagcgt cgccagcacg
      841 ccgcatcctc ccgtaactca cggaaagact agcgcaaatt ctctggaaaa ccgttaacgg
      901 acaaaccgct tgcccgctca ggccaaagcc cctatcccta gggataaaat aaaccacgat
      961 cagggcgcgc gatctcctta catgttcagc tttctggcca atcccgaacg cttcagccgc
     1021 gtgacgcgcc ccttgcgtcc ttggctggcc ggactgtgcg cgctgtgcct gctgtggggg
     1081 ctttatctgg ccttcgcctc gccggaggac tatcagcagg gcgatacggt gcgcatcatg
     1141 tacgtgcatg tgccgtcggc gtggatgggg ctgctgatct atgcgctgat gggcgtcgcg
     1201 tcgtttttcg ggctgatctt ccgtcacgcg ctggccgacg cggcggccaa ggcgatggcg
     1261 ccggtcgggg cggtgttcac cggccttgcg ctgctcaccg gcgcgctgtg gggctggccg
     1321 atgtggggca cgccgtggca gtgggacggg cgcatgacct cggagctggt gctgttcctg
     1381 ttctatgtcg cctatatcgc gctgcacgcc tcgatcgagg acgagacgcg cgccgcgcgc
     1441 atgagcgcga ttctggcgct ggtcggcctc gtcaacctgc cgatcgtgca ttattccgtg
     1501 ctgtggtgga acgggctgca tcagggcaac cgcatcgaaa cgaccggcgc gatcctgtgg
     1561 ccgctgttcg tgatgacggc ggcgtggcac cttttgttcg cgtggctgtt tctggtgcgc
     1621 ctcgacgccg agattctgga gcgccgttac cgggccttgc gcgcccgcct gaattaccag
     1681 tgaggacgcc gtgacgctcg atctcgacat gggcaagtat gctttgttcg tctggggcag
     1741 ctacggcgcg accttcgcgg cgctgacggc gctggtcgtc gtgtcgctgc gcgcgcaggc
     1801 ggcgcggcgc aggcggctcg aagcgctgcg gcaggcggcg gaggacgtaa catgaagcgc
     1861 ctgatctacg ccctgccgct gatcctgttc gccgggctgc tggccgtgct ggcctggtac
     1921 aatttccaca agaaggcgag ttacgagccg cgcgccatgg tcggcaaggc cgtgccggag
     1981 atcgtgctga acgatccgca gggcgggccg ccgtccgacc tgaaaagcct gctggcgggc
     2041 cggacgcggc cggtgctggt caacgtcttc gcctcgtggt gcgtgccgtg catggccgag
     2101 aatccgcaac tgatggcgct gaaggcgaag ggcgtcgtca tcgtcggcgt ggcgtggaag
     2161 gacaagccgc aggacgcgct gaacttcctg gcccagcacg acaaccccta tgtcaagacg
     2221 ctgagcgatc cgaacggcga catggcgctg gccctcggca tttccggcgt gccggagacc
     2281 tatatcgtcg cgccggacgg gacgatcacc gacaagatca ccggccccat cgtgcccgac
     2341 acgctcgatt cggtgctgaa agaggcgcgt taagctgcca tctttgggtc gcc
//
LOCUS       DASVLL010000261         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_68640, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000261 DASVLL010000000
VERSION     DASVLL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68640"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VF402_04375"
     CDS             complement(<1..241)
                     /locus_tag="VF402_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FliL"
                     /protein_id="HEX7799562.1"
                     /translation="MAKKDKNKDADAPPVDGDEAEGGEAAPKKKFPMLIVIIAAAAVL
                     VLGGGGAAAYFLFLAPKHPPAAGAAKADAAKAAKKK"
     gene            537..1286
                     /gene="flgF"
                     /locus_tag="VF402_04380"
     CDS             537..1286
                     /gene="flgF"
                     /locus_tag="VF402_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX7799563.1"
                     /translation="MDNTSYIALSRQITLQRELDISANNLANMNTTGYKFEELLVGAT
                     PGAPAYDAPIRTPANFAYDNGVGRDFSQGTMNQTGAPLDLAINGEGAFFEVATPNGTA
                     YTRDGAFTLDASGTIVDQNGDAVQGDGGPIVLDPKNGEPVISKDGIVTQMFQGQAQRV
                     GKINLVRIANMSDLRKNGDGTYSLTSNAQPVPATDAQIEQGFLEASNVNPMKEVTHLI
                     QINRAYQNLANIIEQNASLSQSAIQRLGKVA"
     gene            1299..2090
                     /gene="flgG"
                     /locus_tag="VF402_04385"
     CDS             1299..2090
                     /gene="flgG"
                     /locus_tag="VF402_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX7799564.1"
                     /translation="MRALRTATTGMAAQQMNVEVISNNIANMNTIGFKKQRAEFQDLI
                     YQNVERMGAQSSDSGTVVPTGIQIGSGVKTGSVYRIMEQGSATLTGNDYDVAIQGKGY
                     FQILLPSGETAYTRAGNFSTDDQGRLVTQDGYLVQPNITIPQGTTSVTISKDGQVQVT
                     TSGNTQTQTLGQLEIATFPNEAGLDAQGDNLFMESAASGAPNVSTPGDTGFGTLLQHY
                     TESSNVDAVSEITALIVAQRAYEMNSKVVTTADSMLQTTSQLARG"
     gene            2103..>2424
                     /locus_tag="VF402_04390"
     CDS             2103..>2424
                     /locus_tag="VF402_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="distal basal body ring component protein"
                     /protein_id="HEX7799565.1"
                     /translation="MKSALLAAALLLIPAAASAQGAATLRAVTTDADGRITLGDLFVN
                     AGAAAHVQVGTRSGPSAVLDAAIVQALAARAGLYWDNPRGLRRIIVTQGPDGDASAPQ
                     SANVA"
BASE COUNT          469 a          867 c          715 g          373 t
ORIGIN      
        1 ctttcttctt cgcggccttg gcggcgtcgg ccttggccgc gcccgccgcg ggcggatgtt
       61 taggcgccag gaacaggaaa taggccgccg ccccgccgcc gcccagcacg aggaccgccg
      121 ccgccgcgat gatgacgatc agcatcggaa actttttctt cggtgcggcc tcgccgcctt
      181 cggcctcgtc gccgtccacc ggaggcgcgt cggcgtcctt gttcttgtcc ttcttagcca
      241 atgcgctcac cgctgcctga aaagttcagg acggcatcct gaacccgaag cttaaacaaa
      301 ccttaaaacg gcccgcgccg tcccttgcgc gatcttcgcc caacatggtt aacgatccgt
      361 tggggcgcgg cgaaaggcgc ggcagttttt gccgcctcca gccttgggca gattcgcggg
      421 ctttcgccat cttttccttt aggcgcaggg atttgcgccc gttaaccctg tctggcacgc
      481 gctttgcatg accctgagca acatggttaa cgccgcaaat tggaaaggag cccggcatgg
      541 acaatacaag ctatatcgcc ctgtcgcgcc agattacgct gcaacgcgag ctggacatct
      601 ccgccaacaa tctggcgaat atgaacacca ccggctacaa gttcgaggag ttgctggtcg
      661 gcgccacgcc gggcgcgccc gcctatgacg cgccgatccg caccccggca aatttcgcct
      721 atgacaacgg cgtcgggcgc gacttctcgc aaggcacgat gaaccagacc ggcgcgccgc
      781 tcgacctggc catcaacggc gaaggcgcct tcttcgaggt cgcgacgccg aacggcacgg
      841 cctacacccg cgacggcgcc ttcacgctgg acgcctccgg cacgatcgtc gaccagaacg
      901 gcgacgcggt gcagggcgac ggcggcccga tcgtgctgga ccccaagaac ggcgaaccgg
      961 tcatctccaa ggacggcatc gtcacccaga tgttccaggg tcaggcccag cgcgtcggca
     1021 agatcaacct cgtgcgcatc gccaatatga gcgacctgcg caagaacggc gacggcacct
     1081 attcgctgac cagcaacgcc cagccggtcc cggcgaccga cgcccagatc gagcagggct
     1141 tcctcgaagc ctccaacgtc aatccgatga aggaggtcac ccacctgatc cagatcaacc
     1201 gcgcctatca gaacctggcc aacatcatcg aacagaacgc ctctttgtcg cagtccgcca
     1261 tccagcggct gggcaaggtg gcgtaggagt aagccgtcat gagagccttg cgcaccgcca
     1321 ccaccggcat ggccgcccaa cagatgaacg tcgaggtcat ctcgaacaac atcgccaaca
     1381 tgaacaccat cggtttcaag aaacagcgcg ccgagtttca ggatctgatc tatcagaacg
     1441 tcgagcgcat gggcgcccag tcctccgatt ccggcaccgt cgtgccgacc ggcatccaga
     1501 tcggctccgg cgtgaagacc ggcagcgtct atcgcatcat ggagcagggc agcgccaccc
     1561 tgaccggcaa cgattacgac gtcgccattc agggcaaggg ctatttccag atcctgctgc
     1621 cgtcgggcga aaccgcctat acgcgcgcgg gcaacttctc gaccgacgat cagggccgcc
     1681 tcgtcaccca ggacggctat ctggtccagc cgaacatcac catcccgcag ggcacgacca
     1741 gcgtgaccat ctccaaggac gggcaggtgc aggtcacgac ctcgggcaac acccagaccc
     1801 agaccctggg tcagctcgaa atcgccacct tccccaacga ggcgggcctc gacgcgcagg
     1861 gcgacaacct gttcatggaa agcgccgcct cgggcgcgcc gaacgtctcg acgcccggcg
     1921 acacggggtt cggcacgctg ttgcagcact acaccgaatc ctcgaacgtc gacgcggtgt
     1981 cggaaatcac cgcgctgatc gtggcgcagc gcgcctatga gatgaactcc aaggtggtca
     2041 ccaccgccga ttcgatgctg cagaccacca gccagcttgc gcgcggataa ggggtcaagg
     2101 ccatgaagtc cgccctcctc gccgccgccc tgctgctgat ccccgccgcc gccagcgcgc
     2161 agggcgcggc gaccttacgc gccgtcacca ccgacgcgga cgggcgcatc acgctgggcg
     2221 acctgttcgt caacgcaggc gccgccgccc atgtgcaggt cggcacgcgc tcaggcccgt
     2281 ccgccgtgct ggacgccgcc atcgtgcagg cgctggcggc gcgcgccggg ctttactggg
     2341 acaatccgcg cggcctgcgc cgcatcatcg tgacgcaagg ccccgacggc gacgcaagcg
     2401 cgccgcaaag cgccaatgtc gcgc
//
LOCUS       DASVLL010000262         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_445938,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000262 DASVLL010000000
VERSION     DASVLL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445938"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..>2332
                     /locus_tag="VF402_04395"
     CDS             168..>2332
                     /locus_tag="VF402_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX7799566.1"
                     /translation="MNTTTSAPQMRRQMRMDIGVSLGLSLVAVFFVLSGAVSYMNIRM
                     LNSDSDKIAATHEVILGLNQILSTMKDAETGQRGFVLTGDEAYLTPYDNALTGIEERV
                     LKVEQETQGQPDFQRRLPPLKSHIAAKMNELAQTISARRESGYQAALDMVRSDRGKAD
                     MDAIRAQVAQMQADAGAERDRRVKEMDHAYDIAVVSVAVTALTGVLLAALITWVISRS
                     QAMRRRESWLQAGQVGLSAASVGEQRLDQLGDSLLDFLAQYLGAEAGVFYAMSEGLYR
                     RVATYGVPEKNDTPAQFRAGDGLLGEAVQSGRAVTLQDLPDNHLSVGAALGQWRPRHV
                     LIAPAAHEGRVEAVVELGFLHAVPPHTLDLMARLSDLMGVAVQSANYRVHLQQLLEET
                     QRQSEELQSQSEELRVNNEELEEQSRALKESHARLEQQQAELEQTNAQLEEQAAILEN
                     QKDDLGRAKSDLEAKARELEQASRYKSDFLANMSHELRTPLNSSLILAKLLSDNPDGN
                     LSEEQVKFARTIQSSGNDLLTLINDILDLSKIEAGQMDIRPEAVSLQRLLRDMERTFE
                     PMAQQKGLKLITRLEGEPPGLIDTDRLRLEQVLKNLLSNAIKFTDKGSVSLTVTAQEA
                     ISGDARVRFIVGDTGIGISKDHQQSIFDAFRQADGTINRRYGGTGLGLSISRELARLL
                     GGRITLDSTPGKGSTFVIDLPVSYDAGAVAPPEVLPVAS"
BASE COUNT          427 a          762 c          785 g          358 t
ORIGIN      
        1 ccgtccggcg cggaccatgt cggcgacggg cggctgagaa tcgctctgat tttacggaat
       61 ttttatttcc gattgcggcc ttcttattgg atgcttgcca cggcgaccct agggtcggat
      121 ttttcaccct gtccgcatcc ccgccggaca ggcgggggag cggctgaatg aatacgacga
      181 cgagcgcgcc tcagatgcgg cgccagatgc gcatggatat cggcgtttcg ctgggcctgt
      241 cgctggtggc ggtctttttc gtgctgagcg gcgccgtctc ctacatgaac atccgcatgc
      301 tgaacagcga ttcggacaag atcgcggcga cgcatgaggt catactgggc ctcaaccaga
      361 ttctctcgac catgaaggac gccgaaaccg gccagcgcgg cttcgtgctg accggggacg
      421 aggcgtatct gacgccgtat gacaacgccc tgaccggtat cgaggagcgc gtgctcaagg
      481 tcgagcagga gacgcagggc cagcccgatt tccagcgccg tctgccgccg ctgaaatcgc
      541 acatcgcggc caagatgaac gaactggccc agaccatcag cgcgcggcgc gaaagcggct
      601 atcaggcggc gctcgacatg gtgagaagcg atcgcggcaa ggcggacatg gacgccatcc
      661 gcgcgcaggt cgcccagatg caggccgacg ccggggccga gcgggaccgc cgcgtcaagg
      721 agatggacca cgcctacgac atcgccgtcg tcagcgtcgc cgtcaccgcc ctgaccggcg
      781 tgctgctggc cgcgctgatc acctgggtga tctcgcgcag tcaggcgatg cggcggcgcg
      841 agtcgtggct gcaggccggt caggtcgggc tgtcggcggc cagcgtcggc gaacagaggc
      901 tggaccagct cggcgacagc ctgctcgatt tcctggccca gtatctcggc gccgaggcgg
      961 gcgtcttcta cgccatgagc gagggactgt accgccgcgt ggcgacctat ggcgtgccgg
     1021 aaaagaacga cacgcccgcc cagttccgcg cgggcgacgg cctgttgggc gaggccgtgc
     1081 agtcgggccg cgccgtgacg ctgcaagacc tgccggacaa tcacctgtcg gtcggcgccg
     1141 cgctggggca atggcggccg cgccatgtgc tgatcgcgcc cgccgcgcac gagggccgcg
     1201 tcgaggcggt ggtcgagctg ggcttcctgc acgccgttcc gccgcacacg ctggacctga
     1261 tggcgcgcct gtccgacctg atgggcgtcg cggtgcagtc ggccaattac cgcgtccacc
     1321 tgcaacaact gctggaggag acgcagcgtc agtcggagga actgcaaagc cagtccgagg
     1381 agttgcgcgt caacaacgag gagttggagg agcagagccg cgcgctgaag gaatcgcatg
     1441 cgcgcctcga acagcagcag gccgagttgg agcagaccaa cgcccagttg gaagaacagg
     1501 ccgccatcct cgaaaaccag aaggacgatc tgggccgcgc caagtccgat ctggaggcca
     1561 aggcgcgcga actggagcag gccagccgct acaagtcgga cttcctggcc aatatgagcc
     1621 acgagctgcg cacgccgctc aattcgtcgc tgatcctggc caagctgctc agcgacaacc
     1681 cggacggcaa tctgagcgag gagcaggtca agttcgcccg caccatccag tcgtcgggca
     1741 acgacctttt gaccctgatc aacgacattc tcgacctgtc caagatcgag gcgggccaga
     1801 tggacatccg cccggaagcg gtgtcgctgc aacgcctgtt gcgcgacatg gagcgcacct
     1861 tcgagccgat ggcgcagcag aagggcctga aactgatcac caggctggaa ggcgagccgc
     1921 ccggcctgat cgacaccgac cggctgcggc tggagcaggt gctgaaaaac ctcctgtcca
     1981 acgcgatcaa gttcaccgac aagggctcgg tcagcctgac cgtcacggcg caggaagcga
     2041 tttcgggcga cgcgcgcgtg cgcttcatcg tcggcgacac cggcatcggc atctcgaagg
     2101 accatcagca gtcgatcttc gacgccttcc gtcaggccga cggcacgatc aaccgccgtt
     2161 acggcggcac ggggctgggc ctgtcgatct cgcgcgagct ggcgcggctg ctgggcgggc
     2221 gcatcacgct cgactccacg ccgggcaagg gttcgacctt cgtcatcgac ctgccggtga
     2281 gctatgacgc gggcgccgtc gccccgcccg aagtcctgcc ggtcgcgtcc aa
//
LOCUS       DASVLL010000263         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_337783,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000263 DASVLL010000000
VERSION     DASVLL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337783"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF402_04400"
     CDS             <1..663
                     /locus_tag="VF402_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX7799567.1"
                     /translation="NVVPSSPATTADLHQLGTGTLSFDSDGNLNLAGSSGPLISATGV
                     TLADSGTTGTAAAPAWAASTGAKGGALSFGFGTGGSSAITQVNSDSTTKSVKANGTEF
                     ASLAKVSIDKNGLVTAVYKNGDTRTIAQVALATFLNSNGLTPVTGNAYQVSTDSGAYL
                     LKVPGSQGAGAIDADTLEASTVDLAQEFTGLITTQRAYSAASKIVTTADQMLQELLSI
                     KQ"
     gene            903..1184
                     /locus_tag="VF402_04405"
     CDS             903..1184
                     /locus_tag="VF402_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX7799568.1"
                     /translation="MLQEQRQDNKGEKYVIGPTGAKLTLNDLPPVGTERWVIRRKAEV
                     VAAVRGGLLTFDEACERYNITSEEFLSWQKAIENHGMAGLRTTRLQSYR"
BASE COUNT          328 a          478 c          496 g          282 t
ORIGIN      
        1 aacgtcgtgc cgtcctcgcc cgcgacgacc gccgacctgc accagctggg caccggcacg
       61 ctgagcttcg acagcgacgg caacctgaat ctggcgggct ccagcggtcc gctcatcagc
      121 gccacgggcg tgaccctggc cgattccggc acgaccggca cggcggcggc gcccgcctgg
      181 gcggcctcga ccggcgccaa gggcggcgcg ctgagcttcg gcttcggcac gggcggctcc
      241 agcgccatca cccaggtcaa cagcgactcg accaccaagt cggtcaaggc caacggcacc
      301 gagttcgcct cgctggccaa ggtcagcatc gacaagaacg ggctggtcac cgccgtttac
      361 aagaacggcg acacgcgcac catcgcgcag gtcgcgctgg ccaccttcct gaactccaac
      421 ggcctgacgc cggtgacggg caacgcctat caggtgtcga ccgattcggg cgcctacctg
      481 ctcaaggtgc ccggctcgca gggcgcgggc gcgatcgacg ccgacacgct ggaagcctcg
      541 acggtcgatc tggcgcagga gttcaccggc ctgatcacca cccagcgcgc ctattcggcg
      601 gcgtccaaga tcgtgacgac ggcggaccag atgctgcaag aattgttgag cattaagcag
      661 taataattaa gtaataatcc ggcgtcatga gtctgtaaaa tgacgccgga ttcatcgtct
      721 gaagcggtat tgataatcat aagaggcgtt aatcttgagg taagctttta tcacatgctg
      781 aacaaagcct taacggtctt gacgcgctgt cgttagccat atgtttacta tggcgattaa
      841 gcggatgtta tgacgcatcg tgtagggtcg gcggcaggtg atgatagtaa aggcaaaaag
      901 ccatgcttca agaacagcgc caggataaca aaggcgagaa atacgtgatc ggtcctaccg
      961 gggcaaaact gaccctcaac gacctgccgc ccgtcggcac ggagcgctgg gtcatccgtc
     1021 gcaaggcgga agtggtcgcc gccgtgcgcg gcggactgct aaccttcgac gaagcgtgcg
     1081 agcgctacaa tattacgtcc gaggaatttc tgagctggca gaaggccatt gaaaatcatg
     1141 gcatggccgg cctgcgcacg acgcggctgc aatcgtaccg ctgaggcgga tcagcgcccg
     1201 ccgcgccgcg cggcggcgga aacttcaggg ccgccgccgg acgaccgggg cggctttcgg
     1261 ttttccggcc attcggaaga tcgctgttat ttgatttaat tcataaatct taacgcgctt
     1321 attaagacct tatttacgca gcactgggaa ataattgccg ggtgataagg gcgccatgcg
     1381 gcgtccgcga tggatggggc aagcgtcagt ggagtctttc ttcagcggtc tgcaacgatt
     1441 cgggatcggc cgtctgacgg cgattctggg cgcggcgggc ggtgtggcgg cggtgatggc
     1501 gatgatcatc ctgcacgtcg cggcgcaacc gcaggcgctg ctctacgcca atctcgacct
     1561 caaggaagcc tcgcagatca cgca
//
LOCUS       DASVLL010000264         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_229982,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000264 DASVLL010000000
VERSION     DASVLL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229982"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF402_04410"
     CDS             complement(<1..515)
                     /locus_tag="VF402_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7799569.1"
                     /translation="MSTARFSDTDRVLIVGGGIGGSLLALVLARQGVRVSVFDPKRTP
                     APSFRNEKLGEEQAEILQALGALEPFTRVCLPPASHPNAYAENPRLTDCGAHHHDWVA
                     AVRAAWPDTVRFVESTVADIETSATRQTVVTGDGTRHEGRLVVLASGRASALHKTLGI
                     EVKTVSAGHSVT"
     gene            complement(655..>1641)
                     /locus_tag="VF402_04415"
     CDS             complement(655..>1641)
                     /locus_tag="VF402_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7799570.1"
                     /translation="DAMAEAVSESYANLSHRYYALKARIMGRKSLDFWDRNAPLSEDV
                     QKSYTWDEAKSIVLGAFAQMGPEFVRRAQLFFDHAWIDAAPRAGKSSGAYAHPVTANR
                     HPYVMLNFTGDRREVLTLAHELGHAVHQTLCQPLGSILADTPLTLAETASIFAEGLVF
                     EHLVASASPEDKKALLAGKIEDGLNSVIRQIAFHRFEEKFHAARQNGEVALDDINAIW
                     LEVMGESLGPAIRLNEGYEVWWAYVSHFVHAPFYVYAYAFGDLLVSALMEKRREDPAA
                     FTPLYEQLLAGGGTKTYVEALAPFGMNPRDKAFWKMGCHRLESLIDQFEALF"
BASE COUNT          280 a          535 c          545 g          281 t
ORIGIN      
        1 gtgaccgaat gaccggcgct gaccgtcttc acctcgatgc ccagcgtctt gtgcaacgcc
       61 gaggcgcggc cggaggccag cacgacgaga cggccttcat ggcgcgtccc gtcgcccgtg
      121 acgacggttt gcctcgtcgc cgaggtctcg atgtcggcga ccgtcgattc gacgaagcgc
      181 accgtgtccg gccacgcggc gcgcacggcg gcgacccagt cgtgatgatg cgcgccgcaa
      241 tcggtcaggc gcggattttc ggcgtaggcg ttcggatgac tggcgggcgg caggcagacg
      301 cgcgtgaacg gctccagcgc gccgagggct tgcaggattt cggcctgctc ctcgcccagc
      361 ttttcgttgc ggaaggacgg ggcgggcgtg cgcttcgggt cgaacacgct taccctgacg
      421 ccctgccgcg ccagaaccag cgccagcagc gacccgccta tgccgccgcc cacgatcagg
      481 acgcggtccg tatcggaaaa gcgggctgtc gacatgaggc gcgtccttcg tctgtcgcaa
      541 aacaggatag agacgaaaag attgcaaaaa tcttgccgac aatctcaggt tgtcaatgct
      601 ttcaggggcg caataaaagc ggctgggcag ggccgggata tggcgcccgc tccctcagaa
      661 caacgcctcg aactggtcga tcaggctttc cagccggtgg cagcccattt tccagaacgc
      721 cttgtcgcgc gggttcatgc cgaagggcgc cagcgcctcg acataggttt tcgtgccgcc
      781 gcccgccaga agctgctcgt acagcggcgt gaaggccgcc gggtcttcgc ggcgcttctc
      841 catcagcgcc gacaccagaa ggtcgccgaa cgcgtaggcg tagacataga agggcgcgtg
      901 gacgaaatgg ctgacatagg cccaccacac ctcatagccc tcgttgagcc tgatcgcggg
      961 gcccagggat tcgcccatga cctccagcca gatggcgttg atatcgtcga gcgcgacctc
     1021 gccgttctga cgcgcggcgt ggaacttttc ctcgaagcgg tggaaggcga tctggcggat
     1081 caccgagttc agcccgtcct cgatcttacc cgccagcagc gccttcttgt cctccggcga
     1141 ggcgctggcc accagatgct cgaacaccag tccctcggcg aagatcgagg ccgtctcggc
     1201 cagggtcagc ggcgtgtcgg ccaggatcga gccgagcggc tggcacaggg tctgatgcac
     1261 cgcgtggccc agttcgtggg ccagggtcag cacctcgcgg cggtcgccgg tgaagttgag
     1321 catcacatac ggatggcggt tggccgtgac cgggtgggcg taggcgcccg acgacttgcc
     1381 cgcgcgcggc gcggcgtcga tccacgcatg gtcgaagaac agttgcgcgc ggcggacaaa
     1441 ttccggcccc atttgcgcga aggcgcccag cacgatgctc ttcgcctcgt cccatgtgta
     1501 cgatttctgc acatcctcgc tgagcggcgc gttgcggtcc cagaaatcga gcgatttgcg
     1561 gcccatgatg cgggctttca gcgcgtaata gcggtgcgac aggttggcgt aggactcgct
     1621 gaccgcctcg gccatcgcgt c
//
LOCUS       DASVLL010000265         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_390647,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000265 DASVLL010000000
VERSION     DASVLL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390647"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VF402_04420"
     CDS             complement(<1..950)
                     /locus_tag="VF402_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7799571.1"
                     /translation="MSLSIFESTQSGFQWVADRVWKVEHAFERANASGEKASSARMRI
                     FIILVGLTFAYVCLAAFAARGALSGDNARMTAITGEPNARAQLVDRNGRLLATDVNDY
                     NMFVDPRDMTAQDRGLVRAALIQLYPGDSDQIEKIFNGHSRVMLNGHIEESDRQRLLS
                     YGLPGVTFEPHRVRDYPLGNTGAAYIGMTMRGGGGMSGAEKALQDQIVAQAVHDQPVQ
                     LAMDLRVQGALQNEVRAAAVDQHALHAVGIVTNVRTGEILGMASWPDFDLNKPAAFTD
                     DQRRNHAAVDRFEVGSIFKVISIGVGLDTGATSMSATYDGR"
     gene            complement(947..1375)
                     /locus_tag="VF402_04425"
     CDS             complement(947..1375)
                     /locus_tag="VF402_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor MinC"
                     /protein_id="HEX7799572.1"
                     /translation="MKGFLRLFERRVRGVRLIELIGLVLVFGMIFWVCLSKAREGDDI
                     RRMNDLDQQIAQEQATVDTLRIKVSQLERPERLEALATEYLNMKPVDPAHETDVGAIG
                     QISRTTSRAPLAPPPAAAPANDLIAASQASASTASNGGAQ"
     gene            complement(1372..>1555)
                     /gene="mraW"
                     /locus_tag="VF402_04430"
     CDS             complement(1372..>1555)
                     /gene="mraW"
                     /locus_tag="VF402_04430"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX7799573.1"
                     /translation="ASRHAPQAARAEAPLYDLITPKAVAASDAEAAANPRARSAKLRA
                     ARRTGAPVPQPKGARR"
BASE COUNT          232 a          509 c          559 g          255 t
ORIGIN      
        1 cgcccgtcat aggtggcgct catcgaggtg gcgccggtgt cgaggccgac gccgatcgag
       61 atcaccttga agatcgagcc gacctcgaaa cggtcgacgg cggcgtggtt gcggcgctgg
      121 tcgtcggtga aggccgccgg cttgttcaga tcgaaatcgg gccacgacgc catgccgagg
      181 atttcgccgg tgcgcacatt ggtcacgatg ccgaccgcgt gcagcgcatg ctgatcgacg
      241 gcggcggcgc gcacctcgtt ttgcagcgcg ccctgaacgc gcaggtccat ggccagttgc
      301 accggctggt cgtggacggc ctgcgccacg atctgatcct gcaaggcttt ttccgcgccc
      361 gacatgccgc cgccgccgcg catggtcatg ccgatatagg ccgcgccggt attgcccagc
      421 ggatagtcgc gcacccggtg cggctcgaag gtgacgcccg gcaggccgta ggacagcagg
      481 cgctgacggt cggattcctc gatatggccg ttcagcatga cgcggctgtg gccgttgaag
      541 atcttttcga tctggtcgct gtcgccgggg taaagctgga tcagggccgc gcgcaccaga
      601 ccgcgatcct gggcggtcat gtcgcgcgga tcgacgaaca tgttgtagtc gttgacgtcg
      661 gtggccagca gccgtccgtt gcggtcgacc agttgcgcgc gcgcgttcgg ctcgccggtg
      721 atggcggtca tgcgcgcatt gtcgccggac aaggcgccgc gcgccgcgaa cgccgccaga
      781 cagacatagg cgaaggtcag gccgaccagg atgatgaaga tgcgcatgcg cgcgctcgac
      841 gccttttcgc ccgaggcgtt ggcgcgctcg aacgcgtgct cgaccttcca gacgcggtcg
      901 gcgacccatt ggaagccgga ctgggtggat tcgaagatcg acaggctcat tgcgcgcccc
      961 cattggaggc cgtggacgcg gacgcctgcg acgccgcgat caggtcgttc gccggggcgg
     1021 cggcgggcgg cggcgccagc ggcgcgcgcg aggtggtgcg cgaaatctgg ccgatggcgc
     1081 cgacatcggt ttcatgcgcc ggatcgaccg gcttcatgtt cagatattcg gtcgccagcg
     1141 cctccagacg ctccgggcgc tccagttgcg acaccttgat cctgagcgtg tccaccgtcg
     1201 cctgctcctg cgcgatctgc tggtcgagat cgttcatgcg ccggatgtcg tcgccttcgc
     1261 gcgccttgga caggcagacc cagaagatca tgccgaacac cagcaccagc ccgatcagct
     1321 cgatcaggcg gacgccgcga acccggcgct cgaacaggcg caggaaaccc ttcatcggcg
     1381 cgcgcctttc ggttgaggaa ccggcgcgcc ggtgcggcgc gcggcgcgca gcttggccga
     1441 gcgcgcgcgc ggattggcgg cggcttcggc gtcggaggcc gccaccgcct tgggggtgat
     1501 cagatcgtaa agcggcgcct cggcgcgagc ggcctgcggc gcgtggcgcg aggcg
//
LOCUS       DASVLL010000266         4990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_500591,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000266 DASVLL010000000
VERSION     DASVLL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500591"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VF402_04435"
     CDS             complement(<1..457)
                     /locus_tag="VF402_04435"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX7799574.1"
                     /translation="MTTGGTKTMKAGDGWRRGLWLAPLSLAVLAFLAYVLPPYLTLDP
                     SRSRIPPPPAHPIYYPILVLHVGCAAIAMASGLAQIWTGVKGAPPGFHRLLGRVYVFC
                     GVLPAGVMALALSTMGQFGPVLIASDMLLAALWLTATITGYRMMRQGRVA"
     gene            complement(575..1465)
                     /locus_tag="VF402_04440"
     CDS             complement(575..1465)
                     /locus_tag="VF402_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7799575.1"
                     /translation="MPRAPLPLNALRAFEAAARHQSFTRAAIELCVTQAAVSHQVKLL
                     EARLRVSLFRRLPRGLMLTAEGEALLPTLRDSFDRMADLLDRFESGFVREVLSVGAVG
                     TFAVGWLVPRLPLLRARHPHIELRLSTNNNRVDLAAEGLDYAIRFGNGAWHDTRAERL
                     FEAPLSVLCTPQIAATLKAPQDVLGHTLLRSYRGDEWPGWLAAAGLPEDALPPRVMAR
                     AMMCDSSLAMVEAAVQGAGIALAPPLMFERLLAAGEIAQPFEVYVSRGSYWLTALQSR
                     PETAAMRAFRAWLLDMAPPA"
     gene            1648..3699
                     /locus_tag="VF402_04445"
     CDS             1648..3699
                     /locus_tag="VF402_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HEX7799576.1"
                     /translation="MRIKTLPLLLLTLLPLCAHAQDRSDTSVTVEGRKKPVKKTADGV
                     SYDVSNSPKAANGTAQDVLQALPGVSVTADGQISVKGQTNVTVLIDGKPSAVMSGPDR
                     AVALQTMNGSDIASVEVITDPSAAYNANGGAILNIVLKKNRNPGAHGSLRGAVSDQGL
                     WNVAASGDATRGKLSLHAGAAFRRDGTLKFRDSAFDWRNPLTGESGATRQSSEVFIRR
                     IVESASLGLDDDLSDKDTLSLNADYHFRRSRPWFDVLNQRSDGSAFHRVSYGPNQQSD
                     AAASATYSHQGDAALKLFAQASNTIALIDKSYRDDWLTPVQPTDYNHGLTKSTRRLDQ
                     LSLDWSRPVRGGQWGLGLDLQDAADDLRNYQAAIDPLTGAETPDPNVSNRYRAATRLD
                     AAYVTRRFDLGRWQWLLGARFEAATMRLSAENFAHRTGWAALDPSLHAQYAVTKATRI
                     SFSYRRSLQRPAPEDLNPFTTYLDAQDRQRGNPDLKPQVMTACELSLDNDTDRLSRSL
                     GLFYRVSRDTVVDTRQVIDGDILMTGKENGGRARSVGATGSLDWTPDDVWSLRADAGA
                     YRVSLNTPDLNGPVRQNALSAYVNLSLGFRKGRDDASLDAHAQSGGIWPLGRYGPTQS
                     LNLSWKRRLTRRLSLTLNANDVLDGGKSVTRMDAATFRMRGYNHFVARRLYVGFVEKL
                     G"
     gene            3928..>4990
                     /locus_tag="VF402_04450"
     CDS             3928..>4990
                     /locus_tag="VF402_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7799577.1"
                     /translation="MIPAMTARGQRGLLRQIRETMEEGQSAQSRLDTVVRIIASSMVA
                     EVCSIYLRRTSEELELFATEGLNADAVHKTRMRLSEGLVGEVARLGQPLNLMDAPSHP
                     SFSYRPETGEDPFKSFLGVPLLRGGRTIGVLVVQNMAARRYEDEEVEDLQIIAMVLAE
                     IVTAGELVGLEELKDIELAPSRSERLKGSIFADGLALGSVVLHEVPVAPDHLLSDDAI
                     AEEARLLYAIDKLRAQIDEMFEGQHGLAGPTFDVLETYRMFAHDRGWVRNLTEAVRSG
                     LTAEAAVERVRNEQRARLNSARDAYMRERLHDLEDLANRLQRVLAGKNTAKRAIPDDA
                     ILVARDLGPADLLEYDRSKL"
BASE COUNT          921 a         1767 c         1613 g          689 t
ORIGIN      
        1 cggcgacgcg gccctggcgc atcatccggt agccggtgat cgtggcggtc agccacaggg
       61 ccgccagcag catgtcgctc gcgatcagaa ccgggccgaa ctggcccatc gtgctcaggg
      121 ccagcgccat gacgcccgcc ggcagcacgc cgcaaaagac atagacgcgg ccgagcaggc
      181 ggtggaagcc cggaggcgcg cccttcacgc cggtccatat ctgcgccagc ccgctcgcca
      241 tcgcgatcgc cgcgcaaccg acatgcagca ccagtatggg ataatagata ggatgcgcgg
      301 gcggcggcgg aatgcgggag cggctggggt ccaatgtcag atacggcggc agcacatagg
      361 cgagaaaggc cagcacggcc aggctcagcg gcgccagcca taatccgcgc ctccatccgt
      421 cgcccgcctt catggttttc gtcccgcccg tggtcatggc cttcttgccc tttcgcgcgc
      481 atccgctgct aattgcgtag catgtacgct taattgcgta tcatgtaaac agaaaatagg
      541 cgcgcggccc tgaacgccgc cggaaacccc aaaatcaggc gggcggcgcc atgtccagca
      601 gccacgcccg gaacgcgcgc atcgccgccg tttcgggccg cgactgcaag gcggtcagcc
      661 agtagctgcc gcgcgagacg taaacctcga acggctgcgc gatctcgccc gccgccagca
      721 ggcgctcgaa catcagcggc ggcgccagcg cgatcccggc gccctgcacg gcggcctcga
      781 ccatcgccag cgaggaatcg cacatcatcg cccgcgccat gacgcgcggc ggcagggcgt
      841 cctccggcag gcccgccgcc gccagccagc ccggccattc gtcgccgcga taggagcgca
      901 acagggtatg gcccagcacg tcctgcggcg ccttcagcgt cgccgcgatc tgcggcgtgc
      961 aaagaaccga cagcggcgcc tcgaacaggc gctccgcgcg cgtgtcatgc caggcgccgt
     1021 tgccgaagcg gatggcgtag tccagcccct cggcggccag atcgacgcgg ttgttgttgg
     1081 tcgacagccg cagttcgata tgcggatggc gcgcgcgcag cagcggcagg cgcggaacca
     1141 gccagccgac cgcgaacgtc ccgaccgcgc cgacgctcag cacctcgcgc acgaagccgc
     1201 tctcgaaacg gtcgagcaga tcggccatgc ggtcgaacga gtcgcgcagg gtcggcagca
     1261 gcgcctcgcc ctcggcggtc agcatcagcc cgcgcggcag ccgccggaac aggctgaccc
     1321 tgagccgcgc ctccagcagc ttgacctgat ggctgacggc ggcctgggtc acgcacagct
     1381 cgatggcggc gcgcgtgaag ctctgatggc gggccgccgc ctcgaaggcg cgcagggcgt
     1441 tgagcggcag aggcgcgcgc ggcatggttc gctccaaatt tttctaatgg ctcatcctac
     1501 ttatcatcgt ttgcgggtgg atagaaccct tgttaaataa ggatatgaag ctcgaaaccg
     1561 caaacagggc gagagctgaa aacttaataa aaagttaatg aaattcatgc cgggcgcagg
     1621 acgcgccgcc ggactgaggg aaacgccatg cgcataaaaa cgctgccgct cttgctgctc
     1681 acgctcctgc cgctctgcgc gcacgcgcag gaccggagcg ataccagcgt cacggtcgaa
     1741 ggccgcaaga agccggtgaa aaagaccgcc gacggcgtca gctacgacgt ctcgaacagc
     1801 ccgaaggccg ccaacggcac ggcgcaggac gtgctgcaag ccctgcccgg cgtgtcggtg
     1861 acggcggacg gacaaatctc ggtcaagggc cagacgaacg tcaccgtgtt gatcgacggc
     1921 aagccgtcgg cggtcatgtc gggaccggac cgcgccgtcg ccttgcagac catgaacggc
     1981 tcggacatcg ccagcgtcga ggtgatcacc gatccgtccg ccgcctacaa cgccaatggc
     2041 ggggcgatcc tcaacatcgt gcttaagaag aaccgcaatc ccggcgcgca cggcagcctg
     2101 cgcggcgccg tttccgatca gggcctgtgg aacgtcgccg cgtccggcga cgccacgcgc
     2161 ggcaagctca gcctgcacgc cggcgccgcc ttccgccgcg acggcacgct gaaattccgc
     2221 gactccgcct tcgactggcg caacccgctg accggcgaaa gcggcgcgac gcggcaaagc
     2281 tccgaggtgt tcatccgccg catcgtcgaa agcgccagcc tcggcctcga cgacgacctg
     2341 agcgacaagg acacgctgag cctgaacgcc gactaccatt tccgccgctc gcgcccatgg
     2401 ttcgacgtgc tgaaccagcg ttcggacgga tcggcgttcc accgcgtctc ctacggcccg
     2461 aaccagcagt ccgacgccgc cgccagcgcg acctacagcc atcagggcga cgccgcgctg
     2521 aaactgtttg cgcaagcgtc gaacaccatc gccctgatcg acaagtccta tcgcgacgac
     2581 tggctgacgc cggttcagcc gaccgattac aaccacggcc tgaccaaatc gactcggcgc
     2641 ctcgatcagc tcagcctcga ctggagccgc ccggtccgcg gcgggcaatg gggcctcggc
     2701 ctcgatcttc aggacgcggc cgacgatctc agaaactatc aggccgccat cgatccgctg
     2761 accggcgccg agacgcccga cccgaatgtc agcaaccgct atcgcgccgc cacccgcctc
     2821 gacgccgcct acgtcacgcg ccgcttcgac ctgggccggt ggcaatggct gctcggcgcg
     2881 cgcttcgagg cggccacgat gcgcctcagc gccgaaaatt tcgcgcacag gaccggctgg
     2941 gccgcgctcg atcccagcct gcacgcgcaa tacgccgtca cgaaagcgac caggatcagc
     3001 ttcagctacc gccgcagcct gcaacgcccc gcgccggaag acctcaaccc gttcaccacc
     3061 tatctcgacg cgcaggaccg ccagcgcggc aatcccgatc tcaagccgca ggtcatgacc
     3121 gcctgcgaac tgagcctcga taacgacacg gaccgcctga gccgcagcct cggccttttc
     3181 taccgcgtca gccgcgacac cgtggtcgac acgcggcagg tgatcgacgg cgacatcctg
     3241 atgaccggca aggaaaacgg cggccgggcg cgctcggtgg gcgcgaccgg ttcgctggac
     3301 tggacaccgg acgatgtgtg gagcctgcgc gccgacgcgg gcgcctatcg cgtcagcctg
     3361 aacacgcccg acctgaacgg accggtgcgg cagaacgccc tgtcggccta tgtcaatctc
     3421 agcctcggct tccgcaaggg ccgcgacgac gcgtcgctcg acgcccatgc gcagtcgggc
     3481 ggaatctggc cgctgggccg ctacggcccg acgcagagcc tgaacctgag ctggaaacgg
     3541 cggctgacgc ggcgcctgag cctcaccctc aacgccaacg acgtgctgga cggcggcaaa
     3601 tccgttacgc gcatggacgc ggccaccttc cgcatgcgcg gctacaacca cttcgtcgcg
     3661 cgccgcctct atgtcggctt cgtggaaaag ctcggttgaa ccgacgtttc agattgttaa
     3721 acggtcgtga taataaccct ttcgtgactt gcgcaaggcg gcgcccagcg actagacacc
     3781 ggcggcgaag cgacacagag taagggacgc gtccgcccga cgcggcgcga agaaggggac
     3841 agcgtggtca gcaaggattc cggtccggga caggagatgg cgaaaacgcc caatgcaatg
     3901 agtccgggcg cgatgatgtc cggcgcgatg atccccgcca tgacggcgcg cggtcagcgc
     3961 ggccttttgc gccagatccg cgagacgatg gaggagggcc agtcggccca gtcgcgcctc
     4021 gacaccgtgg tgcgcatcat cgcctcgtcg atggtggccg aggtctgctc gatctatctg
     4081 cgccgcacct ccgaggaact ggagctgttc gccacggagg gcctgaacgc cgacgccgtg
     4141 cacaagacgc ggatgcggct ctcggagggt ctggtcggcg aggtcgcccg tctgggccag
     4201 ccgctgaacc tgatggacgc gccgtcgcac cccagcttct cctaccgccc cgaaaccggc
     4261 gaagacccgt tcaagtcctt cctcggcgtg ccgctcctgc gcggcgggcg caccatcggc
     4321 gtgctggtcg tgcagaacat ggccgcgcgc cgctacgaag acgaggaggt cgaggatctc
     4381 cagatcatcg ccatggtgct ggccgagatc gtcacggcgg gcgaactggt cggccttgag
     4441 gaactcaagg acatcgaact ggcgccgtcg cgctcggagc ggctgaaagg ctcgatcttc
     4501 gccgacgggc tggccctggg cagcgtggtg ctgcacgagg tgccggtggc gcccgaccac
     4561 cttctgagcg acgacgccat cgccgaggag gcgcgcctgc tctacgccat cgacaagctg
     4621 cgcgcccaga tcgacgagat gttcgagggc cagcacggtc tggccgggcc gaccttcgac
     4681 gtgctggaaa cctaccgcat gttcgcgcac gaccgcggct gggtgcgcaa cctgaccgag
     4741 gcggtgcgct ccggcctgac cgccgaggcc gccgtcgagc gcgtgcgcaa cgaacagcgc
     4801 gcccggctca acagcgcgcg cgacgcctat atgcgcgagc ggctgcacga tctcgaagac
     4861 ctcgccaacc gcctgcagcg cgtgctggcg ggcaagaaca cggcgaagcg cgccattccc
     4921 gacgacgcta ttctggtcgc gcgcgaccta ggccccgccg acctgctgga atacgaccgc
     4981 agcaagctga
//
LOCUS       DASVLL010000267         5895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_446509,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000267 DASVLL010000000
VERSION     DASVLL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446509"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..680)
                     /locus_tag="VF402_04455"
     CDS             complement(138..680)
                     /locus_tag="VF402_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7799578.1"
                     /translation="MALKIEQRIGIAAPVDEVYDLLADIESWPSWSPIHTAASGRVGF
                     GAPVRLEEHYEGLGTWEQSGAVADYQPLSHIHILIPKPFYAGRLIRYFELDILSEAGT
                     GFAVGALFDGFLSEREGRRYRKYLREGFHAFAEALKARAEAQYDPEKPHARINVPEPP
                     AVPKLPPAWKRQTHFWGKKK"
     gene            complement(680..2089)
                     /gene="cysS"
                     /locus_tag="VF402_04460"
     CDS             complement(680..2089)
                     /gene="cysS"
                     /locus_tag="VF402_04460"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7799579.1"
                     /translation="MSLSIHDTLRGEKVAFVPRDPKRVTLYVCGPTVYNYAHIGNARP
                     VVVFDVLFRLLRAMYGEDHVVYARNITDVDDKINRKAMDEGVDISVITERYADIYNAD
                     MAALNALPPTFQPRATAHMGEMVAMIEDLIARDMAYVADGEVLFRVAAWDRGEGRYGR
                     LSKRSLDDMIAGARVEVAENKDAAADFVLWKPSKPGEPVWPGPKGLPGRPGWHIECSA
                     MSEAVLGLPIDIHGGGQDLIFPHHTNEIAQSCAAHGHDDPGSYARYWMHNGFLDFSGE
                     KMSKSLGNVVLAHDLIEDWPGEVIRLALLSAHYRAPLNWTPDLLEQTRARLDGLYGAL
                     RKHAAVSVEDVAAPDAVMAALCDDLNTAKALAELSGLVRTLETAADEAGRVAAKSALL
                     AAGRLLGVLQQNPEQWFKHGASDDLSAQVEALLRQRAEARAAKNWAEADRIRDALNAL
                     NVEVMDGPQGATWKLKAVV"
     gene            2379..3035
                     /gene="folE"
                     /locus_tag="VF402_04465"
     CDS             2379..3035
                     /gene="folE"
                     /locus_tag="VF402_04465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX7799580.1"
                     /translation="MDSLDSRAAPAINENGLLGASKPKSVVTFTNRPSREQAMEAVRT
                     LLAWAGDDPEREGLIDTPKRVVDAYEEWFQGYRADPAKELSRTFEDVQGYDDMVMLRE
                     IDVESHCEHHMAPFLGKAWVAYMPTSKVVGISKIARVVEIFAKRLQTQETMTKQIADA
                     IEDSLAPLGVAVMIDAEHQCMSTRGVHHKNVTTVTTRFTGVFKTDLALQERFLRLTHK
                     "
     gene            3219..3665
                     /locus_tag="VF402_04470"
     CDS             3219..3665
                     /locus_tag="VF402_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7799581.1"
                     /translation="MGAKLGGGGGGKYTVKQNSDINVTPFVDVMLVLLIIFMVAIPAA
                     TVSIKLDLPPAKSPPSNEKKHDPGCISIMGPEDIYIGSQKTSMATLIPDVAAALERTD
                     PGTPLSEQRVLVRGDKDIHYEDFVKVLNKLQQNGYVKVGLITEDVI"
     gene            3739..4839
                     /locus_tag="VF402_04475"
     CDS             3739..4839
                     /locus_tag="VF402_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7799582.1"
                     /translation="MSDSPATDDFDDDLPDSEPDSEDGATLLTAEIGDAAAGERLDRA
                     LAAAFSDLSRARLQALIGEGRLSFGGETIRDGKHKARPGLYELSVPAPISATPEPQDI
                     PLEVLYEDEHLIVVNKPAGMAAHPAPGTPDGTLVNALLFHCRDSLSGVGGVLRPGIVH
                     RLDKDTSGVLVAAKTDATHRALSELFARHDIDRAYNAVVRGAPRYVKGTLNTRIGRSH
                     HDRKKMAVLRGGGREAVTHYTVDEVFGDTKPLAALLTCKLETGRTHQIRVHMAHLGCP
                     CLGDPVYGSGAPHRDVQAILKDLGFTRQALHAAVLGFVHPVSGRKLRFRAPLPADMAA
                     LIDALRAMYGGDANEGEDWDDEDWSLEDGGDA"
     gene            5237..>5895
                     /locus_tag="VF402_04480"
     CDS             5237..>5895
                     /locus_tag="VF402_04480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEX7799583.1"
                     /translation="MLAKDEEFMLAKRWQEHQDPQAAHKLVTSHLRLVAKIAMGYRGY
                     GLPVGEVISEGNVGLMQAVKKFDPERGFRLATYAMWWIKASIQEYVLRSWSLVKMGTT
                     AAQKKLFFNLRKAKSQIAAFQEGDLRPEQVSAIATKLGVLDSEVISMNRRLSGPDASL
                     NAPLRADGESEWQDWLEDNDAVSQETQVAESQEKSLRMSLLEAAMTELTDRERHILTE
                     RR"
BASE COUNT         1035 a         2000 c         1866 g          994 t
ORIGIN      
        1 tcgacatgga tgctggagcc gcacagtttc gacacgcggt cggcctggcc gtccggcgcg
       61 gccagatggc cgacatgccg caactgcgtc gtgcggcgca ggatttcacg cgaatacagg
      121 tcgtcgatca tgccggctta cttcttcttg ccccagaaat gcgtctggcg cttccacgcc
      181 ggcggcagct tcggcacggc gggcggctcc ggcacgttga tgcgcgcgtg cggcttttcc
      241 gggtcgtact gcgcctcggc cctggctttc agcgcctcgg cgaaggcatg gaagccctcg
      301 cgcagatatt tgcggtagcg ccgcccctcg cgctccgaca ggaagccgtc gaacagcgcc
      361 ccgaccgcga agccggtccc ggcctccgac aggatatcca gctcgaaata gcggatcagc
      421 ctgcccgcat agaacggctt ggggataagg atatggatgt gcgacagggg ctggtagtcg
      481 gcgacggcgc cgctctgctc ccacgtgccg aggccctcgt aatgctcctc cagccgcacc
      541 ggcgcgccga aaccgacccg gcccgacgcc gccgtgtgga tcggactcca cgacggccag
      601 ctttcgatat cggcgaggag gtcgtaaacc tcgtccaccg gagcggctat gcctatgcgt
      661 tgttcgatct tgagcgccat cagaccacag ccttcagttt ccaggtcgcg ccttgcggcc
      721 cgtccatcac ctcgacattg agcgcgttca gcgcgtcgcg gatgcggtcg gcctcggccc
      781 agttcttcgc cgcgcgcgcc tcggcgcgct gacgcagcag ggcctcgacc tgcgccgaca
      841 gatcgtcgga cgcgccgtgc ttgaaccact gttccgggtt ctgttgcagg acgccgagca
      901 accgcccggc ggccagcagg gccgacttgg cggcgacgcg gcccgcttcg tccgccgccg
      961 tctccagcgt gcggaccagc cccgacaact cggccagcgc cttcgccgtg ttgagatcgt
     1021 cgcacagcgc cgccatcacg gcgtcgggcg ccgcgacatc ctcaaccgac acggcggcgt
     1081 gcttgcgcag ggcgccgtaa agcccgtcca gccgcgcgcg cgtctgctcc agcaggtccg
     1141 gcgtccagtt gagcggcgcg cgataatgcg ccgacaacag cgccagccgg atcacctcgc
     1201 ccggccagtc ctcgatcagg tcatgcgcca gcacgacatt gccgaggctc ttgctcatct
     1261 tctcgccgga aaagtcgaga aagccgttgt gcatccagta gcgcgcgtaa gatcccggat
     1321 cgtcgtggcc gtgcgcggcg cacgactggg cgatctcgtt ggtgtggtgc gggaagatca
     1381 gatcctgccc gccgccgtga atgtcgatcg gcaggcccag caccgcctcg ctcatggccg
     1441 aacattcgat atgccagccc ggacgccccg gcaatccctt cggtcccggc cacaccggct
     1501 cgcccggctt ggacggcttc cacagcacga aatcggcggc ggcgtccttg ttttcggcca
     1561 cctcgacgcg cgcgcccgcg atcatgtcgt ccagcgagcg cttggacaac cggccgtaac
     1621 gtccctcgcc ccggtcccac gccgcgacgc ggaacagaac ctcgccgtcc gcgacatagg
     1681 ccatatcgcg cgcgatcaga tcctcgatca tcgcgaccat ctcgcccata tgcgcggtgg
     1741 cgcgcggctg gaaggtcggc ggcagcgcgt tcagcgccgc catgtcggcg ttgtagatgt
     1801 cggcgtagcg ctcggtgatg acggaaatat cgacgccttc gtccatcgcc ttccggttga
     1861 tcttgtcgtc gacatcggtg atgttgcgcg catagacgac atggtcctcg ccatacatcg
     1921 cgcgcagcag ccggaacagc acgtcgaaca ccacgaccgg ccgcgcattg ccgatatggg
     1981 cgtaattata gacggtcggg ccgcagacat agagggtcac gcgcttcgga tcgcgcggaa
     2041 cgaacgcgac tttttcgccg cgcagggtgt cgtgaataga aagactcata ggttccgctg
     2101 gcgaaatcgg ttgtaataac gggccatcct cccatataga caccgcgcag tcctgatgcc
     2161 acgttaaatt ggcgccacgc tgaatctcgc tctttcctga gccggtcgcg tcgaaatggc
     2221 gtataaggtc tgcgcgccgg tcgtccgttg ctgacggaag ccggtccgtc ccggcggcag
     2281 gatcgccggc gggacgtctg tcatgaggga tgccacgcgt catttccgaa gccttcgctt
     2341 cggcgccact cggccctcaa gggaattgta gattgaatat ggatagtctg gactcgcgcg
     2401 ccgccccggc catcaacgaa aacggcctgc tgggggcctc caagcccaag agcgtcgtca
     2461 ccttcaccaa ccgccccagc cgcgaacagg cgatggaagc cgtgcgcacc ctgctggcct
     2521 gggcgggcga cgacccggag cgcgaagggc tgatcgacac gcccaagcgc gtcgtcgacg
     2581 cctatgagga atggttccag ggctatcgcg ccgacccggc gaaggaattg tcgcgcacct
     2641 tcgaggacgt gcagggctat gacgacatgg tcatgctgcg cgagatcgac gtcgaatcgc
     2701 actgcgagca tcacatggcg ccgttcctgg gcaaggcgtg ggtcgcctac atgccgacca
     2761 gcaaggtcgt cggcatctcc aagatcgcgc gcgtggtcga aatcttcgcc aagcgcctgc
     2821 agacgcagga gaccatgacc aagcagatcg ccgacgcgat cgaggattcg ctggcgccgc
     2881 tgggcgtggc ggtgatgatc gacgccgagc atcagtgcat gtcgacgcgc ggcgttcacc
     2941 acaagaacgt caccacggtc accacgcgct tcaccggcgt gttcaagacc gatctggcct
     3001 tgcaggaacg cttcctgcgg ctgacgcaca aataggctgg aaatctgtcg ccgcttgcgg
     3061 ctttttcgcc acagaaggcc tttacattta atctcgcctc ggtctaaatc gccgccagcg
     3121 gtttgttaac agggccggcg cgccccggtt tcgggaccga cagcaagaat ttcgccccgc
     3181 gacggtgtaa ggttcgcggc gcatcaaggg agtgtttcat gggcgcaaag ctcggcggcg
     3241 gtggtggcgg caagtacacc gtcaaacaaa attcggatat caacgtgacg cccttcgtgg
     3301 acgtcatgct cgttctgctc atcatcttca tggtggccat tccggccgcg accgtgtcga
     3361 tcaagctcga cctgccgccg gcgaagtcgc cgccctcgaa cgaaaagaag cacgatccgg
     3421 ggtgcatttc gatcatgggg cctgaggaca tctatatcgg ctcgcaaaag accagcatgg
     3481 ccacgctgat ccccgacgtg gcggcggcgc ttgagcgcac cgatccgggc acgcccctgt
     3541 cggaacagcg cgttctggtc cgcggcgaca aagacatcca ttacgaagac ttcgtgaagg
     3601 tgttgaacaa gctgcaacaa aacggctacg tcaaggtcgg cctgatcacc gaagacgtca
     3661 tctaagctcc ggctccgcgc cgttgcgtca aaaacaccgg tcgccgctgg cgtccggtgt
     3721 tttcatttgg aaagccccat gtccgattcg cccgcgaccg acgatttcga cgacgacctg
     3781 cccgatagcg agcccgatag cgaggacggc gcgacgctgc tgaccgccga gatcggcgac
     3841 gcggcggcgg gcgagcggct ggaccgcgcg ctggcggcgg cgttcagcga cctgtcgcgc
     3901 gcgcgcctgc aagccttgat cggcgagggc cgcctcagct tcggcggcga gacgattcgc
     3961 gacggcaagc acaaggcgcg gcccggcctc tacgagttgt cggttcccgc ccccatcagc
     4021 gcgaccccgg agccgcagga cattccgctg gaggtgctgt acgaggacga acacctgatc
     4081 gtggtcaaca agcccgccgg catggccgcg cacccggcgc ccggcacgcc cgacggcacg
     4141 ctggtcaacg ccctgctgtt ccattgccgg gactcgctgt ccggcgtcgg cggggtgttg
     4201 cgccccggca tcgtccaccg gctcgacaag gacacgtccg gcgtgctggt cgcggccaag
     4261 accgacgcga cccaccgcgc cctgagcgag ttgttcgcgc gccacgacat cgaccgcgcc
     4321 tacaacgccg tggtgcgcgg cgccccgcgc tacgtcaagg gcacgcttaa cacccgcatc
     4381 ggccgctcgc accacgaccg caagaagatg gcggtgttgc gcggcggcgg gcgcgaggcg
     4441 gtcacccatt atacggtcga tgaggttttc ggcgatacga agccgctggc cgcgcttctg
     4501 acctgcaagc tggagaccgg ccgcacgcac cagatccgcg tgcatatggc gcatctgggc
     4561 tgcccgtgcc tcggcgatcc ggtctatggc tccggcgcgc cgcatcgcga cgtgcaggcg
     4621 atcctgaaag acctcggctt tacacgtcag gccctgcacg ccgccgtcct cggcttcgtc
     4681 catccggtca gcggcaggaa actgcgcttc cgcgcccccc tgcccgccga catggccgcc
     4741 ctgatcgacg ccttgcgcgc catgtacggc ggcgatgcga atgagggcga ggactgggat
     4801 gacgaggact ggagccttga ggacggcggc gacgcctgat ccgcgtcatc gattgtgcgc
     4861 cgcactcacc cataagtgat gccaggcgcc gcgccggacg tgataatctg ttcacctccg
     4921 tacagttttt cacggccggg gtcttttttt cgccggaagc gtacctaaat cttagcgcag
     4981 ttgtcgggca taagtgtcgt cgaccctgct gcgctccccc ttcgcagact caggagacgg
     5041 tcttatgtcc gacacgattt ctccttctgg ctctccttcc gacatgtcag attcccgatc
     5101 cgaaagcgaa acgccgatga cgacttccgt ggccctgtcc ccgtcgagca accttagcgg
     5161 caatgtcggc agcctgccgt ccctgacgcc cgacggcggc ctgaaccgct acctgtcgga
     5221 gatccgcaag tttccgatgc tggccaagga cgaggagttc atgctggcca agcgctggca
     5281 ggagcatcag gatccgcagg cggcgcacaa gctggtgacc tcgcatctgc gcctcgtggc
     5341 caagatcgcc atgggctatc gcggctacgg cctgccggtc ggcgaagtga tctccgaggg
     5401 caatgtcggc ctgatgcagg cggtcaagaa gttcgacccg gaacgcggct tccgcctcgc
     5461 cacctacgcg atgtggtgga tcaaggcgtc gattcaggaa tatgtgctgc gctcgtggtc
     5521 gctggtgaag atgggcacga ccgccgcgca gaagaagcta ttcttcaacc tgcgcaaggc
     5581 caagagccag atcgccgcct tccaggaagg cgacctgcgc cccgagcagg tcagcgccat
     5641 cgccaccaag ctgggcgtgc tggacagcga ggtcatctcg atgaaccgcc gcctgtcggg
     5701 ccccgacgcc tcgctgaacg cgcccctgcg cgccgacggc gagagcgagt ggcaggactg
     5761 gctggaggac aacgacgcgg tcagccagga gacccaggtc gccgagagcc aggagaagtc
     5821 cctgcggatg agcctgctcg aagccgccat gaccgagctg accgaccgcg agcgtcacat
     5881 cctgaccgag cgccg
//
LOCUS       DASVLL010000268         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_446531,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000268 DASVLL010000000
VERSION     DASVLL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446531"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VF402_04485"
     CDS             <1..591
                     /locus_tag="VF402_04485"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7799584.1"
                     /translation="AGPSELLCIADDTANVKLVAADLLSQAEHDPDAQVILVATSRAV
                     TEAIEAEVAVQVERLPRAQIAKASLDQARAFIVDTPEDAAEVSNLYGPEHLALQVADV
                     DALIPRLTAAGTVFAGTYAAETFGDYAAGPSHVLPTDGAAKAYDGITVRSYLTSFVVQ
                     KATRAGAAAIAPAAARLARLEGLEAHALAADFRLEI"
     gene            686..>2046
                     /locus_tag="VF402_04490"
     CDS             686..>2046
                     /locus_tag="VF402_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7799585.1"
                     /translation="MFNSPFSSLNATGAGLESLPPSLEPLRERMADFYGVEPHSLMVT
                     RGASHATEVLMRRLRVHGHEFVCAQPDHYLDDLGAVYNLSVRQPPENGHLPKGAGFLL
                     IHNPRQTDGRAWDMVAARTLAVDIFPSLLVIDESYFDACTADSLVELAVTEPNVVVLK
                     SLSYLFGMAGARVGALIAGTKTLQGLLKFCEPHPVPTPSVRAAEAALSPSRALSVASR
                     IDTIRAEQARLREALSRSNLLDSFDLNDGPFMLLRPKALIPTQTALKKLGLSAQVTPN
                     GLLLAMGDVATNNRILTALGVAPAGKAPRVGEVLRDTKETKIAVSVNLDQPRPVAIRT
                     GNGFFDHMLDQVATHGGFSLQVACDGDLEIDPHHTIEDCMLAFGQALKLALGDRAGMA
                     RFGFVLPMDETEAKVSVDLGGRPYCVFEGTFAGAHIGDYPTEMTAHAFRSLSETLGAS
                     IHV"
BASE COUNT          310 a          749 c          659 g          328 t
ORIGIN      
        1 gccgggccgt cggagctgct ctgcatcgcc gacgacaccg ccaatgtgaa gctggtcgcc
       61 gccgaccttc tgtcgcaggc cgaacacgat cccgacgcgc aggtcatcct cgtcgccacg
      121 tcgcgcgccg tgaccgaagc catcgaggcc gaggtcgccg ttcaggtcga gcgcctgccg
      181 cgcgcgcaga tcgccaaggc ctcgctcgat caggcgcgcg ccttcatcgt cgacacgccc
      241 gaagacgccg ccgaggtttc caacctctac ggccccgaac acctcgccct tcaggtcgcg
      301 gacgtcgacg cgctcatccc gcgcctgacc gccgccggga ccgtcttcgc cggcacctac
      361 gccgccgaaa ccttcggcga ctacgcagcg gggccgagcc acgtcctgcc gaccgacggc
      421 gcggcgaaag cctatgacgg catcaccgtg cgttcgtacc tgaccagctt cgtggtgcag
      481 aaggcgaccc gcgcgggcgc cgccgccatc gcccccgccg ccgcccgttt ggcccggctc
      541 gaaggtttag aagcccacgc tctggcggcg gacttccgac tggagattta acgcatgatg
      601 ccgttttgcg ccgccagaat ttttgtttgc gctaacgctt cgctgcttga gcgcggggct
      661 gctgatttcc ggttagaggt ctgatatgtt caactcgccc ttctcatcgc tgaacgccac
      721 gggcgcgggg ctggagtcgc tgccgccgtc gctggagccg ctgcgcgagc gcatggccga
      781 cttctacggc gtcgagccgc acagcctgat ggtgacgcgc ggcgcctcgc acgccacgga
      841 ggtgctgatg cgccgcctgc gcgtgcatgg ccacgaattc gtctgcgccc agcccgacca
      901 ctatctcgac gacctcggcg ccgtctataa cctctccgtg cgccagccgc cggaaaacgg
      961 ccacctgccc aagggcgcgg gcttcctgct gatccacaat ccgcgccaga ccgacggccg
     1021 cgcctgggac atggtcgccg cgcgcacgct ggcggtcgac atcttcccca gcctgctggt
     1081 catcgacgag agctatttcg acgcctgcac ggcggacagt ctggtcgaac tggcggtcac
     1141 cgagccgaac gtggtggtgc tgaaaagcct gtcctacctg ttcggcatgg ccggggcgcg
     1201 cgtcggggcg ctgatcgccg gaaccaagac cttgcagggc ctgctgaaat tctgcgagcc
     1261 gcatccggtg ccgaccccgt cggtgcgcgc cgccgaggcc gcgctgtcgc cgtcgcgcgc
     1321 gctcagcgtc gcctcgcgca tcgacaccat ccgcgccgag caggcccgcc tgcgcgaggc
     1381 cttgtcgcgc tcgaacctgc tcgactcgtt cgacctgaac gacgggccgt tcatgctgtt
     1441 gcggcccaag gcgctgatcc cgacccagac ggcgctcaaa aagctcggcc tgtcggcgca
     1501 ggtgacgccg aacggcttgc tgctggccat gggcgacgtg gcgacgaaca accgcatcct
     1561 gacggctctg ggcgtcgcgc ccgcgggcaa ggcgccgcgc gtcggcgaag tcctgcgcga
     1621 caccaaggag accaagatcg ccgtctcggt caatctcgac cagccgcggc cggtcgccat
     1681 ccgcaccggc aacggcttct tcgaccacat gctcgatcag gtcgccaccc acggcggctt
     1741 ctcgttgcag gtcgcctgcg acggcgatct ggagatcgac ccgcaccaca ccatcgagga
     1801 ctgcatgctg gccttcggtc aggcgctgaa actggcgctg ggcgaccggg ccggtatggc
     1861 gcgcttcggc ttcgtgctgc cgatggacga gacggaggcc aaggtcagcg tcgatctggg
     1921 cggacggccc tactgcgtgt tcgaggggac gttcgcgggc gcgcacatag gcgactatcc
     1981 gaccgagatg accgcccacg ccttccgctc gctgtcggaa acgctcggcg cgtcgatcca
     2041 tgtcga
//
LOCUS       DASVLL010000269         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_338298,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000269 DASVLL010000000
VERSION     DASVLL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338298"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF402_04495"
     CDS             <1..474
                     /locus_tag="VF402_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX7799586.1"
                     /translation="AHDIPFTVVDFNPQVTKRARARGLEAWYGNASVPDFLSRLGLQR
                     ARAMVVTVSNPGFTEDVVRTVREMRDDVHIIARARDAAHAQTLYRLGATDAVPETIEA
                     SLQLAENTLIDLGVPMGLVLASVHERRDVFRRQFGSRDGQHDAGALRGRRLAKGA"
     gene            561..1259
                     /locus_tag="VF402_04500"
     CDS             561..1259
                     /locus_tag="VF402_04500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7799587.1"
                     /translation="MTNRSDNRRTFLAAGLAAAGLSLAACSKKAPPPPPPDPPPPPPP
                     PPPPPPKTVTVRLETELGDIDIKLENKKAPITAGNFLKYVDAGKLNGADFWRAADSGP
                     SGFIQATANGPTFPPIAHEPTSQTGLSHTNGAISMSRFAPGTATADFVLCVGDNTFMD
                     AGRSGSSDKLGYAAFGHVVAGMDVVKKILHGRIDPKTPPQGGWAGQMLLHPVKIIKAE
                     RIAEEPDTDAASSR"
     gene            complement(1384..1749)
                     /locus_tag="VF402_04505"
     CDS             complement(1384..1749)
                     /locus_tag="VF402_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX7799588.1"
                     /translation="MGEPKHRLKYAQARRLRRALTPPEARLWLRLKGRQDGLHFRKQH
                     PVGPFIVDFYCAAARLVVEVEGLVHDLPDVAERDAARTAWLESQGLEVMRIPASDIMR
                     DPDEMALGVILRAKEKAGR"
BASE COUNT          313 a          670 c          628 g          302 t
ORIGIN      
        1 gcgcacgaca tcccgttcac cgtggtcgat ttcaacccgc aggtcaccaa gcgcgcccgc
       61 gcgcgcggcc ttgaggcgtg gtacggcaac gccagcgtgc cggacttcct gagccggctg
      121 ggccttcagc gcgcccgcgc catggtggtc accgtctcga accccggctt caccgaggac
      181 gtggtgcgca ccgtgcgcga gatgcgcgac gacgtgcata tcatcgcccg cgcccgcgac
      241 gccgcgcacg cccagaccct ttacaggctg ggcgccaccg acgcggtgcc ggaaaccatc
      301 gaggcgtcgc tgcaactggc tgagaacacg ctgatcgatc tgggcgtgcc gatgggcctg
      361 gtgctggcca gcgtgcatga gcggcgcgac gtcttccgcc gtcagttcgg ctcgcgcgac
      421 ggccagcacg acgccggggc cttgcgcggg cggcggctgg ccaagggggc gtgaggcggg
      481 agacgaaggt ttctcacttt cgccttgcca tccgcgccgc gtttttctac cccttacgcg
      541 aagacgaggg agttttgcgc gtgacgaaca gaagcgataa ccgccgcacc tttctggccg
      601 ccgggctggc cgccgcgggg ttgagcctcg ccgcctgctc gaaaaaggcg ccgccgcctc
      661 cgccaccgga cccgccgccc ccgccgccgc cgcccccgcc gccgccgccg aaaacggtga
      721 cggtgcggct ggagacggag ctgggcgata tcgacatcaa gctggagaac aagaaggcgc
      781 cgatcacggc gggcaacttc ctcaaatatg tcgacgcggg caagctgaac ggcgcggact
      841 tctggcgcgc ggcggattcc ggcccgtcgg gctttattca ggcgacggcc aacggcccga
      901 ccttcccgcc gatcgcgcac gagccgacca gccagaccgg cctgtcgcac accaacggcg
      961 ccatctcgat gtcgcgcttc gcccccggca cggcgacggc ggacttcgtg ctgtgcgtcg
     1021 gcgacaacac cttcatggac gcgggcaggt ccggttcgtc cgacaagctc ggctacgccg
     1081 ccttcggcca tgtcgtcgcg ggcatggacg tggtgaagaa gatcctgcat ggtcggatcg
     1141 accccaagac cccgccgcag ggcggctggg ccgggcagat gctgctgcac ccggtcaaga
     1201 tcatcaaggc cgagcggatc gccgaggagc cggataccga cgcggcttcg tcgcggtagg
     1261 gaagggctga cccctccgtc gtttgctttg caaacgcccc ctccccacga tgtggggagg
     1321 agagtggcct tctcctcccc acatcgtggg gagggggacc gcgaagcggt ggagggggtg
     1381 gacttagcgc ccggccttct cttttgcccg cagaataacg cccagagcca tctcatcggg
     1441 atcgcgcatg atgtccgacg ccggtatgcg catgacctcc agaccctgac tctccagcca
     1501 cgccgtgcgc gccgcatcgc gttcggccac atcgggcagg tcgtggacca gaccctcgac
     1561 ctcgacgacc agccgcgccg ccgcgcaata aaagtcgacg atgaacggac cgaccgggtg
     1621 ttgtttgcga aagtgcagtc cgtcctgacg ccctttcagc cgcagccaca ggcgcgcttc
     1681 cggcggggtc aatgcacgcc gcaaccgccg cgcctgcgcg tatttcagtc gatgcttcgg
     1741 ctcccccatg ggctgagcct atgacgatat gagatggatg caaccgcctt tctctccttc
     1801 ccacatcgta gggaggggaa ccttgcgggc ggaaagatac ccctccgtcg tttgctgtgc
     1861 aaacgccacc tccccacagg gtggggagga gaggggcgtt ttactttccg ctg
//
LOCUS       DASVLL010000270         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_15893, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000270 DASVLL010000000
VERSION     DASVLL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15893"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VF402_04510"
     CDS             <1..588
                     /locus_tag="VF402_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX7799589.1"
                     /translation="RLWLGHASNVSPHYDGYDNLACVIAGRRRFTLYAPELTGRLYPG
                     PIDNTLAGQPVSLAAAADPPDPARYPLFEAAREQALVAELAPGDALYLPKLWWHKVEG
                     LAPVNALVNYWWDATASGPDAPNTAMLLAMIAIAERPEAERRAWRAFFDHYVFRPDGH
                     PLRHLPEDRHGLLGPLRQTYGQIRAHVMRKLRGGA"
     gene            585..2108
                     /locus_tag="VF402_04515"
     CDS             585..2108
                     /locus_tag="VF402_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7799590.1"
                     /translation="MTGPLRSLVILGGGTAGWMAAASLSHFLKPLGVAITLIESEAIG
                     AVGVGEATIPPIMNFIRSLGMDEDEIVRACRATFKLGIAFRDWTRLGHSYLHPFGATG
                     FDMQGVPFYSVWRKARVEGRADDFDAYSLMAQAIKKGRFMRPSGARTSPLHGVTYALH
                     LDASLFAQCLRRFAEARGVRRVEGRVANVALHPETGFIERLDLENGARVDGDFFIDCS
                     GFRGLLIEEALKTGYEDWTRWLPCDRAVAVQSASDAPPPPHTLAVGREAGWQWRIPLQ
                     HRVGNGYVYCSGFIDDDAAQAKLMARLDGPAVSAPRPLRFVTGRRRRMWSRNCLSLGL
                     ASGFLEPLESTGLHLVHRGLAVFLSLLPDRACAPANTERYNRIFAGEYERIRDFLVLH
                     YTAVEREDTPFWRHCRHLPQPDSLQERIELFRAYGRVMPEDNELFPVQSWQYVLTGQG
                     IAPASTDPMAARLDPGHVAEVLDELRAVVGRCADLMPAHGDFLDRNNLKTKLTPMEG"
     gene            2110..2622
                     /locus_tag="VF402_04520"
     CDS             2110..2622
                     /locus_tag="VF402_04520"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEX7799591.1"
                     /translation="MKLLPCLLALAVLFAASARAEPRTDAPPKLSFVFEETVLLGATL
                     TPGATAMGKRFIVPITGGHFEGPGDGSGLRGKVLPGGWDWQLLRADGCLNVKADYMLQ
                     TDDGVIVNVVNTGPLCPGRPVLTTPIFEPPLGKYQWLGQSAFVGALEVVKVDGQPAVR
                     IRFYRVDADQ"
     gene            2648..3607
                     /locus_tag="VF402_04525"
     CDS             2648..3607
                     /locus_tag="VF402_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799592.1"
                     /translation="MMAKSFQRPAAGMLMTICLAVSLAVCLAVFLAAGPAGASAADAP
                     AVEQGSGPFAALYETDPGLPRHVVYRPKDLAADTGKLGVYIFGNGACSADGTASRNHL
                     LEIASHGYLVIASGVIPSARNTPPEPKIAPDGSLNPPTTAKDLTDAIDWALRENSRPG
                     SPYYGRIAPDEIAVSGWSCGGLQALAVAGDPRIRTLVIMNSGVIGDGSLRVADMPLSK
                     ADLSKIHGSVLYVLGGPADIAYKNGTDDYSRIDGLPAALVNIPVGHGGTYMQPHGGLA
                     AEVVTRWLDWRLKGDAAAARDFAGPDCHYCHDPRLTLDRKGFQ"
BASE COUNT          537 a         1301 c         1244 g          583 t
ORIGIN      
        1 cgcctgtggc tgggccacgc ctcgaacgtg tcgccgcact atgacggcta cgacaatctg
       61 gcctgcgtga tcgccgggcg gcggcgcttc accctctatg cgccggagct gaccggccgg
      121 ctctatcccg gccccatcga caacacgctg gcgggccagc cggtcagtct ggccgccgcc
      181 gccgatccgc ccgacccggc gcgctatccc ctgttcgagg cggcgcggga gcaggcgctg
      241 gtcgccgagc tggcgcccgg cgacgcgctc tacctgccca agctgtggtg gcataaggtc
      301 gaggggttgg cgccggtcaa cgctttggtc aactactggt gggacgccac cgcgtccggg
      361 ccggacgcgc ccaacaccgc catgctgctg gccatgatcg ccatcgccga gcgccctgag
      421 gccgagcgcc gggcgtggcg cgcctttttc gaccactacg tcttccggcc cgacggccat
      481 ccgctgcgcc acctgcccga agaccggcac ggcctgctgg ggccgctgcg ccagacctac
      541 ggccagatcc gcgcccatgt gatgcgcaag ctgcggggcg gcgcatgacc ggccccttgc
      601 gcagcctcgt catcctgggc ggcggcacgg cgggctggat ggccgcggcc agcctgtcgc
      661 acttcctcaa gccgctcggc gtcgcgatca ccctgatcga gtccgaggcg atcggcgcgg
      721 tgggggtcgg cgaggcgacc attccgccga tcatgaactt catccgctcg ctgggcatgg
      781 acgaggacga gatcgtgcgc gcctgccgcg ccaccttcaa gctgggcatc gcgttccgcg
      841 actggacgcg gctgggccac agctatctgc atccgttcgg cgccaccggc ttcgacatgc
      901 agggcgtgcc gttctattcg gtgtggcgca aggcgcgcgt cgaaggccgg gccgacgatt
      961 tcgacgccta ttcgctgatg gcgcaggcga tcaaaaaggg gcgcttcatg cggccgtccg
     1021 gcgcgcgcac ctcgccgctg catggcgtca cttatgcgct gcacctcgac gccagcctgt
     1081 tcgcgcaatg cttacgccgc ttcgccgagg cgcgcggcgt gcgccgcgtc gaaggcaggg
     1141 tggcgaacgt cgcgctgcat cccgaaaccg gcttcatcga gcgcctcgat ctggagaacg
     1201 gcgcgcgcgt cgacggcgac ttcttcatag attgcagcgg ctttcgcggc ctgctgatcg
     1261 aggaggcgct gaagaccggc tatgaggact ggacgcgctg gctgccctgc gaccgcgccg
     1321 tcgccgtgca gagcgccagc gacgcgccgc cgccgccgca caccctggcc gtcgggcgcg
     1381 aggcgggctg gcaatggcgc atcccgttgc agcaccgcgt cggcaacggc tatgtctatt
     1441 gcagcggctt catcgacgac gacgcggcgc aggcgaagct gatggcgcgg ctggacggcc
     1501 cggcggtcag cgcgccgcgc ccgctgcgct tcgtcaccgg acgccgccgc cggatgtgga
     1561 gccgcaactg cctgtcgctg gggctggcct ccggctttct cgaaccgctg gaatcgaccg
     1621 ggctgcatct ggtgcatcgc ggtctggccg tgttcctgtc gctgctgccc gaccgggcct
     1681 gcgcgcccgc caacaccgag cgctacaacc gcatcttcgc cggcgaatac gagcgcatcc
     1741 gcgatttcct cgtgctgcac tataccgccg tcgagcgcga ggacacgccg ttctggcgcc
     1801 attgccgcca tctgccgcag ccggactcgt tgcaggagcg catcgagctt ttccgcgcct
     1861 atggccgcgt tatgcccgag gataacgagc tgttcccggt gcagagctgg caatatgtgc
     1921 tgaccgggca ggggatcgcg cccgcctcta ccgacccgat ggcggcgcgg ctcgatccgg
     1981 gccatgtcgc cgaggtgctg gatgaactgc gcgccgtggt cggcaggtgc gccgacctca
     2041 tgcccgcgca cggcgatttc ctcgaccgga acaatctgaa gaccaaactg acgccgatgg
     2101 agggctgata tgaagctttt accgtgtctc ctcgccctgg ccgtcctctt cgccgcatcc
     2161 gcgcgggcgg agccgcggac cgacgcgccg ccgaagctga gcttcgtttt cgaggagacg
     2221 gtgctgctcg gcgccacgct gacgccgggc gcgaccgcga tgggcaagcg cttcatcgtg
     2281 ccgatcaccg gcggccattt cgaggggccg ggcgacgggt cgggcctgcg cggcaaggtt
     2341 ctgccgggcg gctgggactg gcagttgctg cgcgccgacg gctgcctgaa cgtcaaggcc
     2401 gattatatgt tgcagacgga cgacggcgtc atcgtcaacg tcgtcaacac cggtcctctg
     2461 tgtccgggtc ggccggtgct gaccaccccg atcttcgagc cgccgctggg caagtatcag
     2521 tggctggggc aatccgcctt cgtcggcgcg ctcgaggtcg tcaaggtcga cggccaaccg
     2581 gcggtgcgca tccgctttta ccgcgtcgac gcggaccaat aagacccttg cggcaggaga
     2641 ctgaacgatg atggcgaaat cctttcaacg cccggcggcg ggcatgctga tgacgatatg
     2701 tctggccgtt tctctggccg tttgtctggc cgtttttctg gccgccggcc cggcgggcgc
     2761 gtccgccgcc gacgcgccgg cggtcgagca aggctccggc ccgttcgcgg cgctgtacga
     2821 gacggacccc ggcctgccgc gccatgtcgt gtaccggccg aaggatctgg cggcggatac
     2881 gggcaagctc ggcgtctata tcttcggcaa cggcgcgtgc agcgcggacg ggacggcgtc
     2941 gcgcaatcac ctgctggaga tcgcctcgca cggctatctg gtgatcgcgt ccggcgtgat
     3001 ccccagcgcg cggaatacgc cgccggagcc gaagatcgcg ccggacggct cgctcaatcc
     3061 gccgacgacg gccaaagacc ttaccgacgc catcgactgg gccctgcgcg aaaacagccg
     3121 ccccggcagc ccctattacg ggcgcatcgc gccggacgag atcgccgtct ccggctggag
     3181 ctgcggcggc ttgcaggcgc tggccgtcgc gggcgacccg cgcatccgca ccctggtgat
     3241 catgaacagc ggcgtgatcg gcgacggttc gctgcgcgtc gccgacatgc cgctgagcaa
     3301 ggccgacctg agcaagattc acggctccgt cctctatgtg ctgggcggcc cggccgacat
     3361 cgcctacaag aacggaaccg acgactacag ccgcatcgac ggcctcccgg cggcgctggt
     3421 caacatcccc gtcggccacg gcggaaccta catgcagccg cacggcggcc tcgccgccga
     3481 agtggtgacc cgctggctgg actggcgcct caaaggcgac gccgccgccg cgcgcgactt
     3541 cgcaggcccc gattgccact actgccacga cccaagactg accctcgacc gcaagggctt
     3601 ccagtaggac cggcgggaag aaccgggcgg cgtccccaaa cgcgttttga cattttcagg
     3661 aaagt
//
LOCUS       DASVLL010000271         4593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_391110,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000271 DASVLL010000000
VERSION     DASVLL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391110"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..395
                     /locus_tag="VF402_04530"
     CDS             219..395
                     /locus_tag="VF402_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799593.1"
                     /translation="MKLTAFTLLMFFVAVALVTASLIGHFSSVPYLSAYQYWLMTGGF
                     GVLALGMIFKGQSS"
     gene            complement(638..814)
                     /locus_tag="VF402_04535"
     CDS             complement(638..814)
                     /locus_tag="VF402_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799594.1"
                     /translation="MKLTAFTLLMFFVAVALVTASLIGHFRSVPYLSAYQYWLMTGGF
                     GVLALGMIFKGQSS"
     gene            complement(916..1602)
                     /locus_tag="VF402_04540"
     CDS             complement(916..1602)
                     /locus_tag="VF402_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX7799595.1"
                     /translation="MWMLALACAFFLALHLMISATSLKDRVIGRIGGPAYYILFSLLS
                     LGGLIWMSVAFGVALGDPMNVVLWSSGPFLRVVGIIGNFFAFLLVVIGYTSPSPTNLA
                     ALWRMPDHPVQGVIRITRHPVLAGVGLWALTHIVCNGNLAAWIFFGSLLALCALGANT
                     IDRKRAALMGEVYDKIKRRTSIVPFVAILEGRAAFDAKELGVVRPLLAASMFSVFLVL
                     HELIFTVRAI"
     gene            complement(1672..2394)
                     /locus_tag="VF402_04545"
     CDS             complement(1672..2394)
                     /locus_tag="VF402_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799596.1"
                     /translation="MPPFLPVLIILSAAVLAILVVLYRLYVSRVEQPKKLEDELREGL
                     GGFRADVQALEPVRKAAAASSYDGDRVIIVRDFGKSPHKPYTMAELMGVEIFVDDKVV
                     GRVTRGGPTRLLDDIAPTVHRVTMRLMFDDPADPDFELVMWDPHDAMTARAEGPRAAL
                     NNARKWFYHVEAVLRRTAAGTKPAKPPAPALIAAPIVAAPAPRLELSDAPRPPKPEAE
                     GPKTEGPKTEGDVLNAPLIPYL"
     gene            complement(2482..3156)
                     /locus_tag="VF402_04550"
     CDS             complement(2482..3156)
                     /locus_tag="VF402_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799597.1"
                     /translation="MAHAARVFLYFFFGGIGLTLIASGLMWWYEASRRLGRALRSSLG
                     KHPDAIVYDLDGKKAAGLDFTAGDLAILWNTGAQGLVFAFDEIDGAELIVDERVVARA
                     QRGESRKVLNETHAQAAHVTLRLMFDDVQTPEFELNLFGDQVSHSPVHARTAAEAVRL
                     GRKWLSHIDAVIKRVPETAPETAPQTSPPAPESVTPPPRREADARPPWEDDDADNAFG
                     DGDFHP"
     gene            complement(3219..4031)
                     /locus_tag="VF402_04555"
     CDS             complement(3219..4031)
                     /locus_tag="VF402_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7799598.1"
                     /translation="MKLATWNVNSVNARLPLVLQWFREAMPDVCALQEIKCVDEKFPV
                     EAFESLGYNCAVFGQKTWNGVAIVSKYPLSDVRRGLPRKDLADDETDEQSRYIEAVVE
                     APTPVRFASIYLPNGNPLGTDKYAYKLRWLERLRAHAASLLPLEEITVLTGDFNIIPA
                     KDDCWNEAVWLDDALYQPAVRRAYQGLKNLGYADAFETHAPDGNRYTFWDYQAGAWQK
                     NQGIRIDHHLLSPQAADRLREVVIHKDTRGRTHDEAKPSDHVPVEIFLQTQS"
     gene            complement(4107..4451)
                     /locus_tag="VF402_04560"
     CDS             complement(4107..4451)
                     /locus_tag="VF402_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799599.1"
                     /translation="MNMRVFLLTIISAASLLAGAASARDYTYRPEDAKLADVHYQPHR
                     YGKVVYERAARSDPPPATPSLRLPDPPLLASRRMAGKSVDGGSYQCVDRKCSCAIFVR
                     DHTQRDNLMRGY"
BASE COUNT          843 a         1455 c         1502 g          793 t
ORIGIN      
        1 ggacgcgcgg ccttcgacgc caaggagctg ggcgtcgtgc gcccgctgct ggccgcctcg
       61 atgttcagcg tctttctcgt gctgcacgaa ctgatcttca ccgtgcgcgc catctaggtg
      121 aagcacggct ggcgggcgcg aaattcccgt tgatcttgtc acgggaattt gaacaaatgc
      181 gtccaagctt gcggatatga ggggccgggc gcgcgtcgat gaagcttacc gctttcacct
      241 tgctgatgtt ctttgtggcc gtcgcgctcg tgaccgccag cctgatcggc catttcagca
      301 gcgtgccgta tctgagcgcc tatcagtact ggctgatgac cggcggcttc ggcgttctgg
      361 ccctggggat gatcttcaag ggccagtctt cgtagggttt cgccacgcaa ggacacgaaa
      421 tccgcgcgta gcgccttgtc gcctgttggt cgtctgacgg ttgtttttta accacggaaa
      481 acacggaaac cacggaaacg ccgcttcgcg gcgtcagaag attagcgatt tccgtggttt
      541 ccgtggttaa aatcttgcgg acacaaaaga cgggcagata cgacaagacg ctacgcgcgg
      601 atttcgtgcc ttttcgtgct ttttgtggca aaaaacctta cgaagactgc cccttgaaga
      661 tcatccccag ggccagaacg ccgaagccgc cggtcatcag ccagtactga taggcgctca
      721 ggtacggtac gctgcggaaa tggccgatca ggctggcggt caccagcgcg acggccacga
      781 agaacatcag caaggtgaaa gcggtaagct tcatcgacgc gcgcccggtc cctcatatcc
      841 gcaagcttag ccgcatttgt tcaaattccc gtgacaagat caacgggaat ttcgcgccgg
      901 cgcgccgtgg ttcgcctaga tggcgcgcac ggtgaagatc agttcgtgca gcacgagaaa
      961 gacgctgaac atcgaggcgg ccagcagcgg gcgcacgacg cccagctcct tggcgtcgaa
     1021 ggccgcgcgt ccttccagaa tcgccacgaa cggcacgata gaggtgcggc gtttgatctt
     1081 gtcgtacacc tcgcccatca gcgccgcgcg cttgcggtcg atggtgttgg cccccagcgc
     1141 gcacagcgcc agcagcgagc cgaagaagat ccacgccgcc agattgccgt tgcagacgat
     1201 gtgggtcagc gcccacagcc cgaccccggc cagcaccgga tggcgcgtga tgcggatgac
     1261 gccctgcacc ggatggtcgg gcatccgcca cagcgccgcc agattggtcg gcgagggcga
     1321 ggtgtagccg atcaccacca gcaggaaggc gaagaaattg ccgatgattc cgacgacgcg
     1381 caggaacggc ccggaactcc acagcaccac gttcatcgga tcgcccagcg cgacgccgaa
     1441 cgccacgctc atccagatca gcccgcccag cgacagcagc gagaacagga tgtaataggc
     1501 cggtccgccg atcctgccga tcacccggtc tttcagcgac gtggcgctga tcatcagatg
     1561 gagggccaga aagaaggcgc aggccagcgc aagcatccac atgcagacga ctccggggtg
     1621 atgtccgatc ccctgcctaa cacgctccgg cccggcgcgc caagccgcag atcacagata
     1681 agggatcagc ggcgcgttca gcacatcgcc ttccgtcttc gggccctccg tcttcgggcc
     1741 ttccgcctcc ggcttcggcg ggcgcggcgc gtccgacagt tccaggcgcg gcgcgggggc
     1801 ggcgacgatg ggcgcggcga taagggccgg cgccggcggt ttggccggct tcgtcccggc
     1861 ggcggtgcgg cgcagtacgg cctcgacgtg atagaaccat ttgcgcgcgt tgttgagtgc
     1921 ggcgcgcggg ccttcggcgc gggcggtcat cgcgtcgtgc ggatcccaca tcaccagctc
     1981 gaaatcggga tcggccgggt cgtcgaacat cagccgcatg gtgacgcggt ggacggtggg
     2041 ggcgatgtcg tccaaaagcc tggtcgggcc gccgcgcgtc acgcgcccga ccaccttgtc
     2101 gtcgacgaaa atctcgacgc ccatcagttc ggccatcgta tagggcttat gcgggctttt
     2161 gccgaagtcg cgcacgatga tcacgcggtc gccgtcatag gacgaggcgg cggcggcctt
     2221 gcgcaccggc tccagcgcct gcacgtccgc ccggaagccg cccagccctt cgcgcagttc
     2281 gtcctccagc ttcttcggct gctcgacgcg cgagacgtag aggcggtaca gcaccaccag
     2341 aatcgccagc acggcggcgg acaggataat caggacgggc agaaacggcg gcacgcgcga
     2401 aacacctgct gagatcgaaa gaaggctgag ttaaccatgt ttttcgacgc aggttaagcg
     2461 ttgttcgctc gacgggcgaa gtcagggatg aaaatccccg tcgccgaagg cgttatcggc
     2521 gtcgtcgtcc tcccacggcg ggcgggcgtc cgcttcgcgg cgcggcggcg gcgttacgga
     2581 ttcgggtgca ggaggcgacg tttgcggggc cgtttcgggc gccgtttccg ggacgcgctt
     2641 gatcacggcg tcgatatgcg acagccattt gcgccccagc cgcacggcct cggcggcggt
     2701 gcgggcgtgg acgggactgt ggctgacctg atcgccgaac aggttcagtt cgaattccgg
     2761 cgtctgcacg tcgtcgaaca tcagccgcag cgtgacgtgc gcggcctgcg cgtgggtttc
     2821 gttcagcacc ttgcggcttt cgccgcgctg ggcgcgggcg acgacgcgct cgtcgacgat
     2881 cagttcggcg ccgtcgatct cgtcgaaggc gaagacgagg ccctgcgcgc cggtgttcca
     2941 taagatcgcc agatcgcccg cggtgaaatc cagtcccgcc gccttcttgc cgtccagatc
     3001 gtacacgatg gcgtccgggt gcttgcccag cgacgagcgc agcgcgcggc ccagacggcg
     3061 cgaagcctca taccaccaca tcagccccga cgcgatcagg gtcaggccga tcccgccgaa
     3121 aaagaaatac aggaaaacgc gcgcggcatg ggccataagg agagtcacgc cagaaacgga
     3181 ttcaaaacgg ataacgaatc cttatcggcc gtcagcggtt aagattgcgt ttgcaaaaag
     3241 atctcaaccg ggacgtgatc cgacggcttg gcctcgtcgt gtgtccggcc gcgcgtatcc
     3301 ttgtggatga cgacctcgcg caggcggtcg gcggcctgcg gcgacagcag gtgatggtcg
     3361 atgcggatgc cctgattctt ctgccacgcc ccggcctgat agtcccagaa ggtgtagcgg
     3421 ttgccgtccg gcgcgtgagt ctcgaacgcg tcggcgtagc cgaggttctt cagcccctga
     3481 taggcgcggc gcacggcggg ctgatacagc gcgtcgtcca gccacaccgc ctcgttccag
     3541 caatcgtcct tggccgggat gatgttgaaa tcgccggtca ggacggtgat ctcctccagc
     3601 ggcagcaggg acgcggcgtg cgcgcgcagc cgctccagcc agcgcagctt gtaggcgtac
     3661 ttgtccgtgc cgagcgggtt gccgttgggc aggtagatcg aggcgaagcg caccggcgtc
     3721 ggcgcctcga cgacggcctc gatatagcgc gactgctcgt ccgtctcgtc gtcggccaaa
     3781 tccttccggg gcaggccgcg ccgcacgtcg ctgagcgggt atttcgacac gatggcgacg
     3841 ccgttccatg tcttttgccc gaagacggcg cagttatagc caagcgattc aaaggcttcg
     3901 acgggaaact tctcatcgac gcacttgatt tcctgcaagg cgcagacgtc cggcatggcc
     3961 tcgcggaacc attgcaggac cagcggcaaa cgggcgttga cggagttgac gttccaggtg
     4021 gcgagcttca tggctcaagc tgtaatcggc gttgcgggta agggcaagac gggaaggcgg
     4081 ccgatcgcga aaaacgcgcc gccgactcaa tagcctcgca tcagattgtc gcgttgcgta
     4141 tgatcgcgga cgaagatcgc gcagctgcac ttccggtcga cgcattgata gcttccgccg
     4201 tcgacgctct tgcccgccat ccggcgcgac gccagcaggg gcgggtccgg caggcgcagg
     4261 cttggcgtcg cgggcggcgg gtccgagcgc gcagcacgct cataaaccac cttgccgtac
     4321 cggtgcggct gataatgcac gtcggccagc ttggcgtcct ctggccggta ggtgtagtcg
     4381 cgcgccgatg ccgcgcccgc cagcagcgat gcggctgaaa tgatcgtcag caggaaaacg
     4441 cgcatattca tcccctccat tatcggaaaa ggataccatc tccgcatgag gcgcgcaatc
     4501 tcagggcgag cgcgccctac aaggcgaaac tggttccgca gccgcaggag gacaccgcca
     4561 gcgggttcag gatcttgaac tgcgagccga cca
//
LOCUS       DASVLL010000272         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_230513,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000272 DASVLL010000000
VERSION     DASVLL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230513"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VF402_04565"
     CDS             <1..742
                     /locus_tag="VF402_04565"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX7799600.1"
                     /translation="AAAAPVAFVGKGVTFDTGGISIKPAEGMEDMKWDMGGSAAVVGT
                     MIALAGRKARVNAVGVVGLVENMPDGNAQRPGDVVKAMSGTTIEVINTDAEGRLVLAD
                     ALWYCQDRFKPQFMIDLATLTGAMIIALGTDYAGVFSNNDQLAERIAAASKAAGENTW
                     RMPLPPQYEKQIDSKIADIRNIGGRPAGSITAALFLQRFVNGLPWAHMDIAPTAWTNE
                     QRIPSAPEGATGFGVRTLNALVAQYYEA"
     gene            745..1236
                     /locus_tag="VF402_04570"
     CDS             745..1236
                     /locus_tag="VF402_04570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX7799601.1"
                     /translation="MAEAHAEPKDGSGPEFWFYHLETRSLEQTLPELLEKTAQRGWRA
                     YVHGDDQRLEALNARLWTYAPQAFLAHGLESDPHPQHQLVLLGASGAPANAPDVYLSV
                     APVNLPDSGAIDSLRRCLIVFEGADDAHLGWARGLWKRLKGEGRALAYWKQNDNGRWE
                     KMQ"
     gene            1395..>1574
                     /locus_tag="VF402_04575"
     CDS             1395..>1574
                     /locus_tag="VF402_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteinase inhibitor i78"
                     /protein_id="HEX7799602.1"
                     /translation="MPVPTRPEPQSQTPATPPAALKPMADDQCGAQPLQYLVGRPRTD
                     IPVPLDPSTRRVVCST"
BASE COUNT          271 a          521 c          546 g          236 t
ORIGIN      
        1 cgccgccgcc gcgccggtgg ccttcgtcgg caagggcgtg accttcgaca ccggcggcat
       61 ctccatcaag cccgccgagg gcatggagga catgaagtgg gacatgggcg gctcggccgc
      121 cgtggtcggg accatgatcg ccctggccgg gcgcaaggcc cgcgtcaacg cggtcggcgt
      181 cgtcggcctt gtcgagaaca tgcccgacgg caacgcgcag cgccccggcg acgtggtcaa
      241 ggccatgtcg ggaaccacga tcgaggtcat caacaccgac gcggaagggc gtctcgtgct
      301 ggccgacgcg ctttggtact gccaagaccg cttcaagccg caattcatga tcgatctcgc
      361 caccctgacc ggcgccatga tcatcgcgct gggcaccgat tatgcgggcg tgttcagcaa
      421 taacgaccaa ctggccgagc gcatcgcggc ggcgtcgaag gcggcgggcg agaacacctg
      481 gcggatgccg ctgccgccgc agtacgaaaa gcagatcgac tccaagatcg ccgacatccg
      541 caatatcggc gggcgtccgg cggggtcgat cacggcggcg ctgttcctgc aacgcttcgt
      601 caacggcctg ccgtgggcgc acatggatat cgcgccgacc gcgtggacca acgagcagcg
      661 catccccagc gcgccggaag gcgcgacggg cttcggcgtg cgcacgctga acgcgctggt
      721 ggcgcagtat tacgaagcct gacgatggcc gaagcgcacg ccgagccgaa ggacggttcc
      781 gggccggaat tctggttcta ccatctggag acgcgctctc tggagcagac actgccggaa
      841 ctgctggaaa agaccgcgca gcgcggctgg cgcgcctatg tgcatggcga cgatcagcgg
      901 ctcgaagcgc tgaacgcgcg gctgtggacc tatgcgccgc aggcgtttct ggcgcacgga
      961 ctggaaagcg atccgcatcc gcagcatcag ctggtcctgc tgggcgcgag cggcgcgccg
     1021 gccaatgcgc cggacgtcta tctgtcggtc gcgcccgtaa atttgcccga ttccggcgct
     1081 atcgattcgc tgcggcgttg cctgatcgtc ttcgagggcg cggacgacgc gcatctgggc
     1141 tgggcgcgcg gtttatggaa gcggttgaag ggcgaggggc gcgcgctggc ctactggaaa
     1201 cagaacgata acggcagatg ggagaagatg cagtgaagcg aatgtcgggc cgaatatcgg
     1261 gccaaatatc gggccatata tcaggcatga tggcggccgc cctggggttg acggcgggtc
     1321 tggcgatttt ggcgggatgc gcttcggcgc cggagtccgc gccgccgccc gttgccgcgc
     1381 atccccggcc cagcatgccg gttcccacgc gtcccgaacc gcaaagccag acacccgcga
     1441 ccccgcccgc cgcgctgaag ccgatggcgg acgatcagtg cggcgcgcag cccctgcaat
     1501 atctggtcgg caggccgcgc accgacattc ccgtgccgct cgatccgtcg acgcgccgcg
     1561 tcgtctgctc gacc
//
LOCUS       DASVLL010000273         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_15946, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000273 DASVLL010000000
VERSION     DASVLL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15946"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1280)
                     /gene="fba"
                     /locus_tag="VF402_04580"
     CDS             complement(243..1280)
                     /gene="fba"
                     /locus_tag="VF402_04580"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081287.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX7799603.1"
                     /translation="MARISLRQLLDHAAEHGYGMPAFNINNMEQGLAIMEAARKTASP
                     VIIQASRGARDYSNDLMLKRMINALVEIYPEIPLCMHQDHGNSVGTCASAIQHGFTSV
                     MMDGSLKADAKTPADYDYNVDVTRRVVEMAHACGVSVEGELGVLGSLETGMGEAEDGH
                     GAEGVLSHDQLLTDPDQAVDFVAKTGVDALAIAMGTSHGAYKFTRKPDGEVLAMHVIT
                     EIHKRLPNTHLVMHGSSSVPQDLQDMINAYGGEIPQTWGVPVEELQKAIKMGVRKINI
                     DTDLRLAITAGIRKTFAEEPSVFDPRTYFKPAYAMMQQVCEDRFEQFGAAGQAPKIKP
                     VGLGAMAQFYL"
     gene            complement(1405..>2094)
                     /gene="pgk"
                     /locus_tag="VF402_04585"
     CDS             complement(1405..>2094)
                     /gene="pgk"
                     /locus_tag="VF402_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7799604.1"
                     /translation="ESMKRELNALDAALGNPQRPTLGIVGGSKVSTKLDLLKNLVGKL
                     DALAIGGGMANTFLYAQGHDVGGSLCEKDLKDTALDILKEAQARGCQILLPLDVVVAQ
                     EFKAHAPSRTQGLDAPLSADDKIFDAGPKTVEQLNAVMAASKTLIWNGPLGVFELPPF
                     DAATNAAAKFAASQTKAGKLVAVAGGGDTVAALNHAGVAGDMTFVSTAGGAFLEWMEG
                     KPLPGVEALQD"
BASE COUNT          329 a          627 c          696 g          442 t
ORIGIN      
        1 accgcgccct cgatggtgcg tttgaccagc ggtttcttgc cctcgacggt gacggtggtg
       61 gtcgcgcctt tgtccttagg ggcgtcctgc gccatcgccg gatggacgcc gagcagcagc
      121 aggagagggg ttgggattat gtgtcgcata tatgcgacgt atgtagcatg cgtgtccaaa
      181 aacggcaagc ccggcgacgt ggagtcgtcg ccgggcttaa aggcgtcagc ttttacagag
      241 gcttacagat aaaactgcgc catcgcgccg aggccgacgg gtttgatctt gggggcctga
      301 cctgcggcgc cgaactgttc gaagcggtct tcgcagacct gctgcatcat ggcgtaggcg
      361 ggtttgaaat aggtgcgggg gtcgaagacg gacggctctt cggcgaaggt cttgcggatg
      421 cccgccgtga tggcgaggcg cagatcggtg tcgatattga tcttgcgcac gcccatcttg
      481 atggcttttt gcagctcctc gaccggcacg ccccaggtct gggggatttc gccgccatag
      541 gcgttgatca tgtcctgcaa gtcctgcggc accgaggacg agccgtgcat gacgaggtgg
      601 gtgttgggca ggcgcttgtg gatttcggtg atgacgtgca tggccagcac ctcgccgtcc
      661 ggcttgcgcg tgaacttgta ggcgccgtgc gaggtgccca tcgcgatggc cagcgcgtcg
      721 acgccggttt tggccacgaa atcgaccgcc tgatccgggt cggtcaaaag ctggtcgtgc
      781 gacagaacgc cctcggcgcc gtggccgtcc tcggcctcgc ccatgccggt ctcaaggctg
      841 cccagcacgc ccagctcgcc ttcgaccgag acgccgcagg cgtgcgccat ctcgaccacg
      901 cggcgggtga cgtcgacatt gtagtcgtag tcggcggggg tcttggcgtc ggctttcagc
      961 gagccgtcca tcatcaccga ggtgaagccg tgctggatcg ccgaggcgca ggtgccgacg
     1021 gaattgccgt ggtcctgatg catgcagagc ggaatttctg ggtagatttc gaccagcgcg
     1081 ttgatcatgc gcttgagcat caggtcgttg gaatagtcgc gggcgccgcg cgacgcctga
     1141 atgatcacgg gcgaggcggt cttacgcgcc gcctccatga tggcgaggcc ctgctccata
     1201 ttgttgatgt tgaaggcggg catgccgtag ccatgctcgg ccgcgtggtc gagcagttgc
     1261 cgcaagctga tacgagccat gatcttgtcc ctctttcatg ctctgtttcg tcatgtcccg
     1321 gaagtggcct tccgggataa ggttctggac gcctgtttta tgtgttttga agcgtccgaa
     1381 gccataaatt gtaaatacgt ttcctcaatc ctgtaaagct tcgacgccgg gcagcggctt
     1441 gccctccatc cattcgagga aggcgccgcc cgccgtcgac acgaaggtca tgtcgcccgc
     1501 cacgcccgca tggttgagcg ccgcgacggt gtcgccgccg cccgcgaccg cgaccagttt
     1561 accggctttc gtttgcgatg cagcaaattt cgccgccgca ttggtcgccg cgtcgaacgg
     1621 tggcaattcg aacacaccca gcgggccgtt ccagatcagg gttttcgagg cggccatgac
     1681 cgcgttgagt tgttcgaccg tcttcggtcc ggcgtcgaaa atcttgtcgt cggcgctgag
     1741 cggcgcgtcc aagccttgcg tgcggctggg ggcgtgggcc ttgaactcct gcgccaccac
     1801 gacgtcgagc ggcagcagga tttggcagcc cctggcctgc gcttccttca ggatgtcgag
     1861 cgcggtgtcc ttcaggtctt tctcgcataa agacccgccg acatcgtggc cctgcgcgta
     1921 gaggaaggtg ttggccatgc cgccgccgat ggccagcgcg tccagcttgc cgacgaggtt
     1981 tttcaacagg tcgagcttgg tcgagacttt cgagccgccg acgatgccga gcgtcgggcg
     2041 ctgcggattg ccgagcgcgg cgtccagcgc gttcagttcg cgcttcatgc tctc
//
LOCUS       DASVLL010000274         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_447004,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000274 DASVLL010000000
VERSION     DASVLL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447004"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF402_04590"
     CDS             complement(<1..279)
                     /locus_tag="VF402_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX7799605.1"
                     /translation="MLRAGSLRAALALLRRDLSLTRGGGPLLAAGFYLTLTAMIPLSL
                     GPDQAVLARIAPGLAWLSLALSSLTALERLFERDYEDGLFDLLRQGPLP"
     gene            complement(272..904)
                     /gene="ccmA"
                     /locus_tag="VF402_04595"
     CDS             complement(272..904)
                     /gene="ccmA"
                     /locus_tag="VF402_04595"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX7799606.1"
                     /translation="MRNVMKLIIEGLRLRKGYNRLVDGLDLVLAPGEALSLSGENGVG
                     KTTLLRAVAGFSPPEAGTIRFEHDGAALEAEAAQARMIHYLGHQDAASPARTVAQELR
                     FQADWLGGDMTGIGPLRLEPLLDLETRYLSAGQKRRLSCARLLMAKRPLWLLDEPMAP
                     LDAAHRDLLAGLMQAHLAQGGSIIAAVHDPLPFATRGLRLIRPVPEAARA"
     gene            complement(926..>1730)
                     /locus_tag="VF402_04600"
     CDS             complement(926..>1730)
                     /locus_tag="VF402_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799607.1"
                     /translation="NFMDLTALILEDSATQAQIIGRMLEADGWHYIHCETVREASEAL
                     TQMQVHALLLDIYVGQHNSLLHIERFKAMAPGAPLIFMSAGGGGADAMQTLSAARRAG
                     AAYVLRKPFTPGTLRGIMGAIEQDRLKGRKRPHVLVIDDSRTVRKMLVNSFKSADYRV
                     SEAGSMEAAFADCDIAHVDLAVCDIFMPGMGGVQGMAMLKQAWPRIGVIAMSAGMDGQ
                     MTEAQALEAAARVGADSCIAKPFTAQVLLLRAESVLKAKQMAQKAGEPA"
BASE COUNT          271 a          611 c          590 g          258 t
ORIGIN      
        1 cggcaggggg ccttgccgca gcaggtcgaa caggccgtcc tcgtaatcgc gctcgaacag
       61 gcgctccagc gccgtcaggc tgctgagcgc gaggctgagc caggccagac ccggcgcgat
      121 gcgggccaga accgcctgat ccgggccgag gctcaggggg atcatcgccg tcagggtcag
      181 gtagaacccc gccgccagca acggcccgcc gccgcgcgtc aacgacaggt cgcgcctgag
      241 cagggccaaa gcggccctaa gcgagccggc cctaagcacg ggcggcctcc gggacgggac
      301 ggatcaggcg caggccgcgc gtcgcgaacg gcagcggatc gtgcacggcg gcgatgatcg
      361 agccgccttg cgccagatgc gcctgcatca gcccggccag caggtcgcgg tgcgcggcgt
      421 cgagcggcgc catcggctcg tccagcagcc agagcgggcg tttcgccatc aaaaggcgcg
      481 cgcaactcag gcggcgtttc tgccccgccg acagatagcg ggtttcgagg tccagcaacg
      541 gttcgagccg cagcgggccg atcccggtca tgtcgccgcc cagccagtcg gcctgaaacc
      601 gcagctcctg cgccacggtg cgcgcgggcg aggcggcgtc ctgatggccg agatagtgga
      661 tcatccgcgc ttgcgcggcc tcggcctcga gcgccgcgcc gtcatgctcg aagcggatcg
      721 ttccggcttc gggcggcgaa aagcccgcga cggcgcgcag aagcgtggtc ttgccgacgc
      781 cgttctcgcc cgacaggctg agcgcctcgc ccggcgccag aacgaggtcc agcccgtcca
      841 ccagccggtt atagcccttc ctcaggcgca gcccctcgat gatcagtttc atgacgttac
      901 gcataggcca agccgccagc cttgcctagg ccggttcgcc cgccttttgc gccatctgct
      961 tggccttcag cacgctttcg gccctgagca gcaaaacctg cgcggtgaac ggcttggcga
     1021 tgcaggaatc ggcgccgacg cgcgcggcgg cctccagcgc ctgcgcctcg gtcatctggc
     1081 cgtccatgcc cgccgacatg gcgatgacgc cgatgcgcgg ccacgcctgt ttcagcatgg
     1141 ccatgccctg caccccgccc atgcccggca tgaagatgtc gcacaccgcc agatcgacat
     1201 gggcgatgtc gcagtcggcg aaggcggcct ccatgctgcc ggcttcggag acgcgatagt
     1261 ccgcgctctt gaagctgttg accagcattt tgcgcacggt gcgcgaatcg tcgatgacca
     1321 ggacgtgcgg gcgcttgcgc cccttgagcc ggtcctgctc gatggcgccc atgatgccgc
     1381 gcagggtgcc cggcgtgaac ggcttgcgca gcacgtaggc ggctccggcg cggcgcgcgg
     1441 cgctcagggt ctgcatggcg tccgcgccgc cgccgcccgc cgacatgaag atcagcggcg
     1501 cgccgggcgc catcgccttg aaacgctcga tatgcagaag cgaattgtgc tggccgacat
     1561 agatgtcgag cagcagcgcg tgcacctgca tctgggtcag ggcctcggac gcctcgcgca
     1621 cggtttcgca atggatgtaa tgccagccgt ccgcctccag catccgcccg atgatctggg
     1681 cctgcgtggc gctgtcttcc agaatgaggg cggtcaggtc catgaaattc
//
LOCUS       DASVLL010000275         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_230887,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000275 DASVLL010000000
VERSION     DASVLL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230887"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VF402_04605"
     CDS             complement(<1..981)
                     /locus_tag="VF402_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7799608.1"
                     /translation="MTQHGGDRFMPHGYCFLWKPDLLWLHVLSDAGIVVAYMGIPLVL
                     LYVLRRRRDLPFQGVFALFGAFILLCGFTHFLNIWVLWRPDYYFEGVVKALTAIASIA
                     TLIALVVRIPLALSLPSPRQLTEANAKLADLYQKSQEQGRAALSVVLDNVTDGIITID
                     ERGQIESFNAACARMFGYGPDEVTGRNIAALIPEPDQKKHEGFVRDYLGAGAFSREMT
                     GRRKDGRKFPLDLSVSDFQLGGRIHYIGILRDISRAKQAEAARERLLTRLTDSNTELE
                     RFAYVASHDMQEPLRMMLNFSQIVAHDYADKLDDDGREYLKIIGDSAWRMR"
     gene            1145..1615
                     /locus_tag="VF402_04610"
     CDS             1145..1615
                     /locus_tag="VF402_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799609.1"
                     /translation="MTRLIERERSAQVLLIEDNRGDAVLTRRAFKRARIANDITVAES
                     GEKGLSILRREGEYAEAPRPDLILLDLNLPYMHGRDFLSTVKDDPHLRRIPVIVLSSS
                     SADADINAAYARHANGYITKPVSSDTYDNVVSRLESYWFELMQMPPDDPIPAFS"
     gene            complement(1620..2240)
                     /gene="alkB"
                     /locus_tag="VF402_04615"
     CDS             complement(1620..2240)
                     /gene="alkB"
                     /locus_tag="VF402_04615"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: protein motif:HMM:NF011930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEX7799610.1"
                     /translation="MQAELFDLSPPGLRVLRGYALSAAPDLLAGIAAIAAAAPFRRMT
                     TPGGQVMSAAMTSCGDLGWITDRRGYRYAAADPLSGRPWPAMPEAFRALAVAAAAEAG
                     FAFAPDSCLINRYEAGARMGLHQDADEPDADAPIVSASLGLPVTFLWGGLGRKDAVTR
                     LALRHGDVAVWGGTMRRAFHGVAPLRGGRHELTGEARYNLTFRKAG"
     gene            complement(2230..>2732)
                     /locus_tag="VF402_04620"
     CDS             complement(2230..>2732)
                     /locus_tag="VF402_04620"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7799611.1"
                     /translation="AVAQSSLGAVLVAESDKGVCAISLGDDPETLMRELQDRFPAARL
                     SGGDADFEARVAQVVGFVEAPRLGLGLPLDIRGTAFQQRVWQALRAIPAGETLSYAEL
                     ARRIKAPAAVRAVAGACAANTLAVAVPCHRIVRADGGLSGYRWGVARKAALLVKEKLV
                     KERDAG"
BASE COUNT          467 a          985 c          876 g          404 t
ORIGIN      
        1 gcgcatccgc caggccgaat cgccgatgat cttcagatat tcgcgcccgt cgtcgtccag
       61 cttgtccgca tagtcgtgcg cgacgatctg gctgaagttc agcatcatgc gcagcggctc
      121 ctgcatgtcg tgcgaggcga cataggcgaa gcgctccagc tcggtgttgg aatcggtcag
      181 ccgcgtcaaa agccgctcgc gcgccgcctc ggcctgcttg gcgcggctga tgtcgcgcag
      241 gatgccgata tagtggatgc gcccgccgag ctggaaatcg gacacggaca ggtccagcgg
      301 gaatttgcgc ccgtccttgc gccgcccggt catttcgcgg ctgaacgccc cggcgccgag
      361 atagtcgcgc acgaagccct cgtgcttctt ctggtcgggt tcgggaatca gcgcggcgat
      421 gttgcggccc gtgacctcgt cggggccata gccgaacatg cgcgcgcagg cggcgttgaa
      481 gctttcgatc tggccgcgct cgtcgatggt gatgatgccg tcggtgacgt tgtccagcac
      541 gacgctgagc gcggcgcggc cctgttcctg gcttttctgg tacaggtcgg ccagcttggc
      601 gttggcctcc gtcaactggc gcgggctggg caggctcagc gccagcggga tgcgcacgac
      661 cagcgcgatc agggtggcga tcgaggcgat ggcggtcagc gccttgacca cgccctcgaa
      721 atagtagtcc ggccgccaca atacccagat gttgaggaag tgcgtgaagc cgcacagcag
      781 gataaaggcg ccgaacagcg cgaacacccc ctggaacggc aggtcgcggc ggcggcgcag
      841 cacatagagc agcaccagcg gtatgcccat ataggccacc acgatgcccg cgtcggacag
      901 gacatgcagc cacagaaggt cgggcttcca caggaagcaa taaccgtgcg gcatgaacct
      961 gtcgccgccg tgctgggtca tggcttatct cccgcctgct ccgcgcgcac tgtctcgcga
     1021 ttttccccgc tcggcaaggg ccgtcgtccg cgcgcgtccg gcttctgtgc atgatgcgga
     1081 aaatgacttg cccgaaggtg gaaaatgagg acaaatcatg cggcgggaaa cgttggcggg
     1141 acggatgact cgactcatag aacgggagcg cagcgcgcag gtgctgctga tcgaagacaa
     1201 tcgcggcgac gccgtgctga cgcggcgcgc cttcaaacgg gcgcgcatcg ccaacgacat
     1261 caccgtggcc gaatcgggcg aaaagggcct gagcatcctg cgccgcgaag gcgaatacgc
     1321 cgaggcgccg cgccccgacc tgatcctgct ggacctgaac ctgccctata tgcacgggcg
     1381 cgacttcctc tcgacggtca aggacgatcc gcacttgcgc cgcatcccgg tgatcgtgct
     1441 gtcctcgtcc agcgccgacg ccgacatcaa cgccgcctat gcgcgccacg ccaacggcta
     1501 catcaccaag ccggtcagca gcgacaccta cgacaacgtc gtctcgcgcc tcgaatcgta
     1561 ctggttcgag ctgatgcaaa tgccccccga cgacccgatc ccggcgtttt cgtaataaat
     1621 tacccggcct tgcgaaacgt cagattatac cgcgcctccc cggtcaactc atgccgcccg
     1681 cccctgagcg gcgccacgcc gtgaaacgcc cgccgcatcg tcccgcccca caccgcgacg
     1741 tcgccgtgcc gcaacgccag ccgcgtcacc gcgtccttgc gccccaaccc gccccataga
     1801 aacgtcaccg gcagccccag cgacgccgac acgatcggcg catccgcgtc cggctcgtcg
     1861 gcgtcctgat gcaggcccat gcgcgccccg gcctcgtaac ggttgatcag gcaggaatcc
     1921 ggcgcgaagg cgaaccccgc ctcggccgcc gccgcgaccg ccagcgcgcg gaacgcctcc
     1981 ggcatggcgg gccacggcct gccgctcagc ggatcggccg ccgcatagcg ataccccctg
     2041 cggtcggtga tccagccgag atcgccgcaa ctggtcatgg ccgccgacat gacctgcccg
     2101 cccggcgtcg tcatgcggcg gaacggcgcg gctgcggcga tggccgcgat cccggccagc
     2161 aggtccggcg cggcgctcag cgcatagccg cgcaatacac gcaaacccgg cggcgacaga
     2221 tcgaacagct cagcctgcat cccgttcctt caccagcttt tccttcacca acagggccgc
     2281 cttgcgcgcc acgccccagc gatagccgga caacccgcca tcggcgcgca cgatgcgatg
     2341 gcacggcacg gccacggcca gcgtgttggc cgcgcaggcc ccggccacgg cgcgcacggc
     2401 ggcgggcgcc ttgatgcgcc gggccagttc ggcgtagctc agcgtctcgc ccgccggaat
     2461 ggcgcgcagc gcctgccaca cgcgctgttg gaacgccgtg ccgcgaatgt cgagcggcag
     2521 gccgaggccc agccgcggcg cctcgacgaa gccgaccacc tgcgcgacgc gcgcctcgaa
     2581 gtccgcgtcg ccgccgctca accgcgccgc cggaaagcgg tcctgaagct cgcgcatcag
     2641 cgtttcggga tcgtcgccga ggctgatcgc gcacacgccc ttgtcgcttt cggccaccag
     2701 cacggcgccg aggctggatt gggcgacggc ga
//
LOCUS       DASVLL010000276         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_16492, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000276 DASVLL010000000
VERSION     DASVLL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16492"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VF402_04625"
     CDS             complement(<1..658)
                     /locus_tag="VF402_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799612.1"
                     /translation="MKLNKALILATTAFAGLCASSLALAQSTATQELETTVVVKGARK
                     SAAGVGVDQTVTKTRSSITDEYLATQAPGSPLDAINILPGVNFTDQDATGSVGFDITL
                     RGFDSQRIALILDGLPLNDTGNYAIYPTMNIDSELYSRIDVNLGSTDVDSPTAAAGGG
                     TINFVTKKPADDFGVEAKETLGDHAYARSFFRLDTGKFGPWGTTAFLTYSNLYSDKWK
                     G"
     gene            complement(1145..2338)
                     /locus_tag="VF402_04630"
     CDS             complement(1145..2338)
                     /locus_tag="VF402_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7799613.1"
                     /translation="MKLTLLAGTALAALAFAGVASAEQTPGWYGALDLGYNSTGDYKA
                     RSTGDMPDGGPYVYNVGTDNDWAGFARLGYRYSPNWRVEFEGGYRPGDIKSAIGFPRT
                     YPTESGLKDTALCTVGTIRGSSDACGGPSGAFTQSTFMVNLYYDILPESNFHPFVGVG
                     VGVDHIKAKMFGQLSTNPAGVISNLTMVGKDTVPAAQFLAGLAWNVSDRLAIDLTYRY
                     LAAQKASFNAGIDPAGGWQQPGEFSGKFDDQTVSVGLRWAFSTPPAPPPPPPPPPPPP
                     PPPPPPPPAYEARQFIVYFEFDKSDLTSDAQAVVQAAADYAKAGNAARIVVVGHTDTS
                     GSAAYNIKLSERRAKTVADALAGLGVDASKLAVDWKVESDPAVATGDGVKEPLNRRST
                     IDITF"
     gene            2742..2831
                     /locus_tag="VF402_04635"
     tRNA            2742..2831
                     /locus_tag="VF402_04635"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2776..2778,aa:Ser,seq:cga)
     gene            complement(2980..>4677)
                     /locus_tag="VF402_04640"
     CDS             complement(2980..>4677)
                     /locus_tag="VF402_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7799614.1"
                     /translation="AASLMSGRGGQAVGVATQLDSLGGDQKNALLLSYPLKAALRAYR
                     PALSALIAIGVLGLVFVVLTSLYLARSLSRPIEALTQAALALREGRGGQIDIVAKDEM
                     GELADAFNDMAASIQERERDITRLAKCDPATGLPNRSAFEDHLQALRDSGVAEQGGVA
                     IVCIGVDRYAHIRAVWGVAYGHTLMARVQDILGGIFAKAYIARLSGDTLGLCLTPCGA
                     GQALPLARAIAARLDKRIRVTDDQSLDVRVSLGVYHLARSRASDDCVERALIALDQAR
                     AAKVKVASFDPRVYSELADTLLLTDQLHAALQNHDMMVHYQPKFSYREGRITSAEALV
                     RWNHAERGFVSPQRFVAIAEETGAIRDLTLQVLQTCIDDQRRLKAEGFDLDISINYSG
                     RLLSDEAFNQRALRLCRQAVGRICLEITETAVIDDPRIGLAAINTFVEAGLEISIDDF
                     GTGLSSLAYLKQIPAHELKIDRAFVMEIESGQRDALLVRSSIDLAHGLGMKVTAEGVE
                     TRAAFTLLQAMGCDLAQGYGIARPMPFEALAPFLSDFNGVVSDVEREPAVGERRRAV"
BASE COUNT          852 a         1461 c         1527 g          837 t
ORIGIN      
        1 agcccttcca cttgtccgaa tacaggttgg aataggtcag aaaggccgtc gtgccccacg
       61 ggccgaactt gccggtatcg aggcggaaga acgagcgggc gtaggcgtgg tcgccgaggg
      121 tttccttggc ttcgacgccg aaatcgtcgg ccggcttctt ggtcacgaag ttgatcgtgc
      181 cgccgccggc cgccgccgtc gggctgtcga cgtcggtgct gcccaggttc acatcgatgc
      241 gcgaatagag ttcgctgtcg atgttcatgg tcggataaat ggcgtagttg ccggtgtcgt
      301 tcagcggcag gccgtccagg atcagggcga tgcgctgcga atcgaaaccg cgcagggtga
      361 tgtcgaagcc gaccgaaccg gtggcgtcct gatcggtgaa gttcacgccc ggcaggatgt
      421 tgatggcgtc cagcggcgag ccgggcgcct gcgtggccag atattcgtcg gtgatcgacg
      481 aacgcgtctt ggtcacggtc tggtcgacgc cgacgccggc ggcggatttg cgcgcgccct
      541 tgacgacgac cgtggtttct aactcctgcg tcgcggtgga ttgcgccagg gccaggctcg
      601 acgcgcacag gcccgcgaag gccgtcgtcg ccagaatgag cgccttattc agtttcatta
      661 tggagtatcc cctctccagg tttacagtca gatgcaaaaa acccacttca ggcgggacgc
      721 tggcgcgtag gggcgaggcc cggccagggt ggccgcgccg caggtctttt gctgatggat
      781 tgcaaaatat cctttagtag gttttgcaac tttgtttgag cgaaaagtgc gtaagtttta
      841 tgacgcttgt ctcttccgca acattgtgac gcagttttgc cacataggtg atcgcggcga
      901 ttcgcgcgcg aacttcgcgc cacgcgcgaa caaagaccag atttagcagg gattacagat
      961 ttcgaccggc atatcttgcg acgcggccgg gccgcttttt cctgtgcgta aaaaccgctt
     1021 ttccgcgcgc gccagcaaaa aacgctccgg cggcttcaca ttgtgttgcg gcgcacaaca
     1081 aaaaacccgg cctgcgcgag gcaggccggg ctcttctttt tcggatcgct atcgcgctct
     1141 taaattagaa cgtgatgtcg atggtcgaac gacggttcag cggttccttc acgccgtcgc
     1201 cggtggcgac cgcaggatcc gattcaacct tccagtcgac cgccagcttg gaggcgtcga
     1261 cgccgaggcc cgccagagcg tcggccacgg tcttggcgcg gcgttccgac agcttgatgt
     1321 tgtaagccgc cgaaccggag gtgtcggtgt gaccgacgac cacgatgcgg gccgcgttgc
     1381 cggccttggc gtagtcggca gccgcttgca ccaccgcttg cgcatccgag gtcaggtcag
     1441 acttgtcgaa ctcgaagtag acaatgaact gacgcgcttc gtacgccggg ggcggcggag
     1501 gcggaggcgg cgggggaggc ggcggggggg gcggaggagg cggaggcgcg ggcggcgtcg
     1561 agaaggccca acgcagaccg acgctcacgg tctgatcgtc gaacttaccc gagaattcgc
     1621 ccggttgctg ccagccgccg gccggatcga tacccgcgtt gaacgaggcc ttttgcgcag
     1681 ccaggtaacg gtaggtcagg tcgatcgcca gacggtccga gacgttccag gccagaccgg
     1741 ccaggaattg cgccgccgga accgtgtcct tgccgaccat ggtcaggttc gagatcacgc
     1801 ccgccgggtt ggtggacagt tggccgaaca tcttggcctt gatatggtcg acgccgacgc
     1861 ccacgccgac gaacggatgg aagttcgatt cgggcaggat gtcgtagtac aggttgacca
     1921 tgaaggtcga ctgcgtgaac gcgcccgacg ggccgccgca ggcgtcgctc gaaccgcgga
     1981 tcgtgccgac ggtgcagagc gcggtgtcct tcagacccga ctcggtcggg taggtgcgcg
     2041 ggaagccgat cgcagacttg atgtcgccgg ggcgatagcc gccttcgaat tcgacgcgcc
     2101 agttcgggct gtaacgatag cccagacgcg cgaagcccgc ccagtcgttg tcggtgccga
     2161 cgttatagac gtaaggaccg ccgtccggca tgtcgccggt cgagcgggcc ttatagtcgc
     2221 ccgtcgaatt atagccgagg tcgagtgccc cgtaccatcc aggcgtttgt tccgcggacg
     2281 caacgcccgc aaacgccagt gcggcgagag cagtgcccgc cagcaaagtg agtttcatgt
     2341 tagtgccctc tcatggccat acgaacgaac agccgataac tcgactggaa ctttagtaca
     2401 ggccttcata actttccgaa gtcgtcttgc atagcaccca aatacgggtt ttggcgaaaa
     2461 tcagcctctt tttatccggt ttggcgagga ctgacacctg aatgcaacgc ttcgacgcat
     2521 gaagccatac tatggctcca tataggtcaa acctcaccat caaaaatcta tagcgccccc
     2581 cggatcgcac ataaaaaacg cataaaggcc gcaaatgggg cttgcgattc cggcaaagtg
     2641 tgagtaggaa cacgcctcct gaccgcgccg ggccgttgca aaacacccgt cagcaaattc
     2701 aaaaccttga tattcaaagc gttttgacag agatcctctg cggagaggtg gctgagtggt
     2761 cgaaagcacc gcactcgaaa tgcggcatag gggcaactct atcgtgggtt cgaatcccac
     2821 cctctccgcc atctcctgca actgtaatcc catactgcaa cgcccgctct cgcggcgccg
     2881 tcgcggatat gaggtcttat aataccgaaa aacgccgtgt ttcgcgccgg tttgtgtgca
     2941 gcgcacacta tcgtcaactc taccttaggg agaatcgcgt cagaccgcgc ggcggcgctc
     3001 gcccacggcg ggttcgcgtt cgacgtcgct gaccacgccg ttgaaatccg acaggaacgg
     3061 cgccagcgcc tcgaagggca tgggccgcgc gatgccgtag ccctgcgcca gatcgcagcc
     3121 catcgcctgc aacagcgtga aggccgcgcg cgtctccacg ccctccgccg tgaccttcat
     3181 gcccagaccg tgcgccagat cgatggacga gcgcaccaga agcgcgtcgc gctggccgct
     3241 ctcgatctcc atgacgaagg cgcggtcgat cttcagctcg tgcgcgggaa tctgcttgag
     3301 ataggccagg gacgacagac cggtgccgaa atcgtcgatc gagatctcca gcccggcttc
     3361 gacgaaggtg ttgatcgccg ccagcccgat gcgcggatcg tcgatcacgg cggtctcggt
     3421 gatctcaagg cagatgcgcc cgaccgcctg acggcacagg cgcagggcgc gctggttgaa
     3481 cgcctcgtcc gacagcaggc ggcccgaata gttgatgctg atgtcgaggt cgaagccctc
     3541 ggccttgagg cggcgctggt cgtcgatgca ggtttgcagc acctgcaggg tcaggtcgcg
     3601 aatcgcgccc gtctcctcgg cgatggcgac gaagcgctgc ggcgagacga agccgcgctc
     3661 ggcgtggttc cagcgcacca gcgcctcggc cgaggtgatc cggccctcgc gatagctgaa
     3721 tttcggctga taatgcacca tcatgtcgtg gttttgcagc gcggcgtgca actggtcggt
     3781 cagcagcagc gtgtcggcca gttcggaata gacgcgcgga tcgaagctgg ccaccttgac
     3841 cttggcggcg cgcgcctgat ccagcgcgat cagcgcgcgc tcgacgcagt cgtccgaggc
     3901 gcgcgaccgc gccagatgat agacgcccag gctgacgcgc acgtcgaggc tctgatcgtc
     3961 ggtgacgcgg atgcgcttgt cgaggcgcgc ggcgatggcg cgggccagcg gcagggcctg
     4021 cccggcgccg cacggcgtca ggcacaggcc cagcgtgtcg cccgacagcc gcgcgatata
     4081 ggccttggcg aagatgccgc cgagaatgtc ctgcacccgc gccatcaggg tatggccgta
     4141 ggccacgccc cacaccgcgc ggatatgggc gtagcggtcg acgccgatgc agacgatggc
     4201 cacgccgccc tgctcggcca cgccgctgtc gcgtagcgcc tgcaagtggt cctcgaaggc
     4261 cgagcggttg ggcaggccgg tcgccggatc gcacttggcc agccgcgtga tgtcgcgttc
     4321 gcgctcctga atcgacgccg ccatgtcgtt gaaggcgtcg gccagttcgc ccatctcgtc
     4381 cttggccacg atgtcgatct ggccgccgcg cccttcgcgc agggccagcg ccgcctgggt
     4441 cagcgcctcg atcggccgcg acagcgagcg cgccagatag aggctggtca gcaccacgaa
     4501 gaccagcccc agcacgccga tggcgatcag cgcgctcagc gccgggcggt aggcgcgcag
     4561 ggcggctttc agcggatagc tgagcagcag cgcgtttttc tggtcgccgc ccagactgtc
     4621 cagttgcgtg gcgacgccga ccgcctgacc gccgcgcccg gacatcaggc tggcggc
//
LOCUS       DASVLL010000277         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_338510,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000277 DASVLL010000000
VERSION     DASVLL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338510"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VF402_04645"
     CDS             complement(<1..413)
                     /locus_tag="VF402_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799615.1"
                     /translation="MNRQAPDLRLKLLSGGLCGAMVMALQACSTAPTPKYPIYMQDKP
                     AEATETAAQAGDGQVAAKSVAAKPVAANPMGGQAGAASSSAAPVAALKGSVSVSDLPP
                     PPPPPEEEDNETAAAPQPARRAAAPVRDTRAAYVYV"
     gene            690..866
                     /locus_tag="VF402_04650"
     CDS             690..866
                     /locus_tag="VF402_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799616.1"
                     /translation="MHDLIPLFRSYWWLMFPTFWVVYGIVRLALRDAYERRRLNLIRT
                     LAEQGRDIPDALRR"
     gene            877..1311
                     /locus_tag="VF402_04655"
     CDS             877..1311
                     /locus_tag="VF402_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799617.1"
                     /translation="MTFANFFAAYWWLIFPIGGMMMGVIAMITHYNHRNEMLKILKSY
                     VDQGKEPPATLLDAMRSDEDRAYDWRWRGRYGRCYGPWNAWRKVIVWGALAAGFTYAG
                     HYTQMGETNGVFSALGVAFTVAAIAALVWALISSFAGPRDPL"
     gene            1338..1856
                     /locus_tag="VF402_04660"
     CDS             1338..1856
                     /locus_tag="VF402_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7799618.1"
                     /translation="MTQPSLIQQAKAGSDIAFSRLVAEHQQAVRAFLRRLCARPDEAD
                     DMAQEAFVTAWANLHRLREDARFRTFVCGIAYRKALNDRRSRGRALRRDSDWSELQPQ
                     SAASGADRRAAVEAALRTLPVEQRAAVSLCVAGDYSHAEAAEILRLPLGTVKSHIMKG
                     RQKLTELLGVAS"
     gene            1853..2161
                     /locus_tag="VF402_04665"
     CDS             1853..2161
                     /locus_tag="VF402_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799619.1"
                     /translation="MTADHDSLKALFLETALPEPPAHDPGFALEVMKRVEKRRLMDGL
                     FALFAAVLTVSAVLFAVMPYLTPVVNNLAAAFAPAIAILVLIGLSMLAFEPVRRMVRI
                     "
BASE COUNT          397 a          778 c          778 g          429 t
ORIGIN      
        1 acatagacat aggcggcgcg cgtgtccctg accggcgcgg cggcgcggcg cgccggttgc
       61 ggcgcggcgg cggtttcgtt gtcctcttcc tccggcggcg gcggcggggg cggcaggtcg
      121 ctgaccgaga ccgagccttt cagcgcggcg accggcgcgg cggatgacga ggcggcgccc
      181 gcctgccctc ccatcgggtt cgcggcgacc ggcttcgcgg cgaccgactt cgcggcgacc
      241 tgtccgtcgc ctgcctgcgc ggcggtttcg gtcgcctcgg cgggcttgtc ctgcatgtag
      301 atcggatatt tcggcgtcgg cgccgtcgaa caggcctgca aggccatgac catcgcgccg
      361 cacagcccgc cgctcaacag tttcagacgc agatcgggcg cttgacgatt catggcgggg
      421 cctccggttt ttaagacttt attcagcgaa attctatgcg aacttatgga taacgaatcc
      481 ttaccgcgcc tcacctttta cgggaaaatc gggccaaaaa acgcccaaaa cgccaggctc
      541 cggtggacaa gcgcgcggac aggcgcgcgg cggacgggaa aaaaatttca cgccgccgca
      601 tccgatgagg cgcttagcgg acatttaagt ctcgaaagcg gcccggcggg tttcagaccc
      661 gaaggccgga aacgagacgg agagacgaca tgcacgacct gatccccctg ttccgcagct
      721 actggtggct tatgttcccc accttctggg tcgtgtacgg catcgtgcgg ctggcgctgc
      781 gcgatgcgta cgagcgccgc cgcctcaacc tgatccgcac cctggccgaa cagggccgcg
      841 acattcccga cgccctgcgc cggtaaggag aacctgatga ccttcgccaa ttttttcgcg
      901 gcctactggt ggctgatctt cccgatcggc gggatgatga tgggcgtgat cgccatgatc
      961 acccattaca accaccgcaa cgagatgctg aaaattctca aatcctatgt cgatcagggc
     1021 aaggagccgc ccgccaccct gctggacgcg atgcgctcgg acgaggaccg cgcctatgac
     1081 tggcgctggc gcggacgcta cgggcgctgt tacggcccgt ggaacgcctg gcgcaaggtg
     1141 atcgtctggg gcgcgctggc cgccggcttc acctatgcgg gccattacac ccagatgggc
     1201 gagacgaacg gcgtcttttc ggcgctgggc gtcgccttca cggtcgcggc gatcgccgcg
     1261 ctggtctggg cgctgatctc gtccttcgcc ggtccgagag accccttgta actctttgag
     1321 tggaatccgg tccttccgtg acgcagccct cgctgattca acaggcaaaa gctggctccg
     1381 acatcgcctt ttcaaggctg gtggcggagc atcagcaggc ggtgcgggcc ttcctgcgcc
     1441 gcctgtgcgc gcgcccggac gaggccgacg acatggcgca ggaggcgttc gtcacggcgt
     1501 gggccaatct gcaccgcctg cgcgaggatg cgcgtttccg caccttcgtg tgcggcatcg
     1561 cctaccgcaa ggcgctcaac gaccgccgca gccggggccg cgcgctgcgc cgcgacagcg
     1621 actggtccga actgcaaccg caaagcgccg cgtccggcgc cgatcggcgc gcggcggtcg
     1681 aggcggcgct gcggaccctg ccggtcgagc agcgcgccgc cgtcagcctg tgcgtggccg
     1741 gggattacag ccacgccgag gccgccgaaa tcttacggct tcccctcggc acggttaagt
     1801 cgcatattat gaagggtcgg caaaagctga ccgaactgtt gggagttgcg tcatgaccgc
     1861 agatcacgat tcgctcaagg ccctgttcct ggagaccgcg ctgccggagc cgcccgcgca
     1921 cgatcccggc ttcgcgctgg aggtcatgaa gcgcgtcgaa aagcgccgtc tgatggacgg
     1981 gctgttcgcc ctgttcgcgg cggtgctgac cgtcagcgcc gtgctgttcg ccgtcatgcc
     2041 ctacctgacg cccgtggtga acaacctggc tgcggccttt gcgcccgcca tcgccatact
     2101 ggtgctgatc ggcctgtcga tgctggcttt cgagcccgtt cgccggatgg tgcggattta
     2161 aaacagatca aggtttagtc acggatgatg gccattcggc catctgcacg gatcgccgct
     2221 tcgcggcgtc acggatagat taagaaggtt tatccacaga tcagcacaga tattccggtt
     2281 cactgcacgc taccgctacc ggcggagcgg gcggctttta taaaggcgct tcgcggccgt
     2341 aatcgaaaac ggcttggacg atagcggtag cgtgaagccg cc
//
LOCUS       DASVLL010000278         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_285664,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000278 DASVLL010000000
VERSION     DASVLL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285664"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VF402_04670"
     CDS             <1..334
                     /locus_tag="VF402_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799620.1"
                     /translation="DQANKTQDDDTNRLVAVYTAMKPKDAAVVMASMKDEVRLPIAAK
                     MKDRALAAILGAMDPTAARDLTEKLATRMQNAGGLQQQLDKASAGGAQASAGAAKTAA
                     NKPAAKKG"
     gene            complement(294..773)
                     /locus_tag="VF402_04675"
     CDS             complement(294..773)
                     /locus_tag="VF402_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799621.1"
                     /translation="MSDLAADPRSYTIYGEARAARGWRPGALSLSLLAGLIVCAASGT
                     VIIERIADLPDIDSLFAPQIVQEAAVAAPPERMTVLAAAAQSDIDIAPTPPVRVEPYA
                     SDYASGDEAPTLPAIYDDAPVEAPAPVMTRAEVAADAQLAQTLEASPSSPQAYSPPS"
     gene            1000..>3241
                     /locus_tag="VF402_04680"
     CDS             1000..>3241
                     /locus_tag="VF402_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7799622.1"
                     /translation="MARLRFLLQPRPAPKAKHPWRAAALVAATVGLGMAHMALGDSMA
                     LPQLASRADIDIRVGRNQQSGRIEIYGAVGSRASVRREHGSDGDQVIIRLPGQQKPDL
                     GDIRANPPLGVAAVDLKADARASELWLKVKPGYDAHFGRADGAVYVQIDPGAEDAEKM
                     DNGKDGKPLAVNLQDLLAPKTASGPGAEHVNRAASAPVVAIQVDNVNGGKQISFPFEG
                     PAAAAVFRRGDSVWIVFDQEVDLRLPPDLKDGLIVQDAQWARNDGFTALRLRAPTAGS
                     LSAIDDGLVWRVQLGGQPPDNKAARISLLRDDSTGVPALNINLAGATRVAWIRDPGVG
                     DRMAVIPARGPIKNLPTARTLMEATIGSTAQGAVVMHMTPDVKVSVDGDLISVTRPDG
                     LTLSNLDPNANASDARLDYKNALYPSLVNPDWSATPDEGFLARYNALQAAAADEAQGG
                     PTGPTKARLSLARFLVGQGLTYEAQGVLDLLARQNPHALDDPQVRGLRVAAKMLSRRY
                     DDALGDLSAPQLISDPAARLWAGYAEAKAGHFPDAVKGFKAGLKALDQFPVSWRMKLG
                     AAYAYAAMQTKDMTTAQAMIGYAVSQDGAPLDKLGAYLVDAQIIEATGDKARALAVYT
                     ACAKASDDAIAAPALMHAAMLRYQLGQANIDQTLNTLDQLRYRWRGDDTELQLITYMG
                     QTYLSAGRYREALQILRSGGQSFLNDPREAQIQASLNQAFRGLFLGGMADGLQPVEAL
                     GLFKDFR"
BASE COUNT          530 a         1107 c         1157 g          447 t
ORIGIN      
        1 cgatcaggcc aacaagacgc aggacgacga caccaaccgg ctggtcgccg tctatacggc
       61 catgaagccg aaggacgcgg cggtggtgat ggcgtcgatg aaggacgagg tgcgcctgcc
      121 gatcgccgcc aagatgaagg accgcgccct ggccgccatt ctgggcgcca tggacccgac
      181 ggcggcgcgc gacctgaccg agaagctggc cacgcggatg cagaacgccg gcggcttgca
      241 gcagcagctc gacaaggcgt cggcgggcgg cgcgcaggcc agcgcgggcg cggctaagac
      301 ggcggcgaat aagcctgcgg cgaagaaggg ctagcctcca gggtctgcgc cagttgcgcg
      361 tccgccgcga cctcggcgcg cgtcatgacc ggcgcgggcg cctccacggg cgcgtcgtcg
      421 tagatggcgg gcagggtcgg ggcttcgtcg cccgacgcat aatccgacgc atagggttcg
      481 acccggaccg gcggcgtcgg cgcgatgtcg atatcgctct gcgcggcggc ggcgagcacg
      541 gtcatgcgct ccggcggcgc ggcgacggcc gcttcctgta cgatctgcgg cgcgaacagc
      601 gagtcgatat ccggcaggtc ggcgatacgc tcgataatca ccgtcccgct ggcggcgcag
      661 acgatcagcc cggccagcag gctgaggctg agcgcgccgg gacgccagcc gcgcgcggcg
      721 cgcgcctcgc cgtagatcgt atagctgcgc ggatcggcgg cgaggtcgga catgatcggg
      781 ctcctgaaaa ctggcgggat cagtctgaca ggcggcgcgc aaagccgcag cacgggccgc
      841 gcgcccgcgc cgtaaaagcg cgatagctgc gattccttac cggttattaa ctccgctgaa
      901 acgcccttca ggcgaaatta accatcgatg attcgtgtgc cgccgccttc gcccgaggac
      961 gcgccagacc cggctgagga agccgcgcgt cgggacgaga tggcgcgcct gcgattcctg
     1021 ctgcaaccgc gcccggcgcc gaaggcgaag catccgtggc gcgcggcggc tctggtggcg
     1081 gcgacggtcg ggctgggcat ggcgcacatg gcgctgggcg actcgatggc cctgccgcaa
     1141 ctggcctcgc gcgccgatat cgacatccgc gtcgggcgca accagcagtc gggccgcatc
     1201 gaaatctacg gcgcggtcgg ttcgcgcgcc tcggtgcgcc gcgaacatgg ttcggacggg
     1261 gatcaggtga tcatccgcct gccgggccag caaaagcccg atctcggcga catccgcgcc
     1321 aatccgccgc tgggcgtcgc cgccgtcgac ctgaaggccg acgcgcgcgc cagcgaactg
     1381 tggctcaagg tcaagcccgg ctacgacgcc catttcggcc gcgccgacgg cgccgtctat
     1441 gtgcagatcg atcccggcgc cgaagatgcg gaaaagatgg acaacggcaa ggacggcaag
     1501 ccgctggccg tcaatctgca agacctgctg gcgccgaaaa ccgcgtccgg gccgggcgcc
     1561 gagcatgtca accgcgccgc cagcgcgccg gtggtggcca ttcaggtcga caatgtcaac
     1621 ggcggcaagc agatctcgtt cccgttcgag ggaccggcgg cggcggcggt gttccggcgc
     1681 ggcgattcgg tgtggatcgt cttcgatcag gaggtcgacc tgcgcctgcc gcccgatctc
     1741 aaggacggcc tgatcgtgca ggacgcgcaa tgggcgcgca atgacggctt cacggcgctg
     1801 cgcctgcgcg cgccgacggc gggcagcctg tcggcgatcg acgacggcct cgtctggcgc
     1861 gtgcagctgg gcgggcagcc gcccgacaac aaggcggcgc gcatctcgct gctgcgcgac
     1921 gattccacgg gcgtgcccgc gctcaacatc aatctggccg gcgcgacgcg ggtggcatgg
     1981 attcgcgatc ccggcgtcgg cgaccgcatg gcggtgatcc cggcgcgcgg cccgatcaag
     2041 aacctgccga ccgcgcgcac cctgatggag gccaccatcg gctcgacggc gcagggcgcg
     2101 gtggtcatgc acatgacgcc ggacgtcaag gtcagcgtcg acggcgacct gatctcggtc
     2161 acgcgcccgg acgggctgac cctgtccaat ctcgatccca acgccaacgc cagcgacgcg
     2221 cgcctcgact acaagaacgc gctctatccc tcgctggtca atcccgactg gagcgcgacg
     2281 ccggatgagg gtttcctggc gcgctacaac gccttgcagg ccgccgccgc cgacgaggcg
     2341 cagggcggcc cgacggggcc gaccaaggcg cggctgtcgc tggcgcgctt tctggtcggg
     2401 cagggcctga cctatgaggc gcagggcgtg ctggacctgc tggccaggca gaatccgcac
     2461 gcgctggacg atccgcaggt gcgcggcctg cgcgtggcgg ccaagatgct gtcccggcgc
     2521 tacgacgacg cgctgggcga cctgtcggcg ccgcaactga tctccgatcc ggcggcgcgc
     2581 ctgtgggcgg gctatgccga ggccaaggcc gggcatttcc ccgacgcggt caagggtttc
     2641 aaggcgggcc tgaaggcgct ggaccagttc ccggtgtcct ggcgcatgaa gctgggcgcg
     2701 gcctacgcct acgccgccat gcagaccaaa gacatgacca cggcgcaggc gatgatcggc
     2761 tatgcggtgt cgcaggacgg cgcgccgctc gacaagctgg gcgcctatct ggtcgacgcg
     2821 cagatcatcg aggcgacggg cgacaaagcg cgcgctttgg cggtctatac cgcctgcgcc
     2881 aaggcgtcgg acgacgccat cgccgcgccc gccctgatgc acgccgccat gctgcgctat
     2941 cagttggggc aggccaatat cgatcagacg ctgaacacgc tggatcagtt gcgctaccgc
     3001 tggcgcggcg acgacaccga actgcaactg atcacctata tgggccagac ctatctgagc
     3061 gccggacgct atcgcgaggc gttgcagatc ctgcgcagcg gcggccagtc cttcctcaac
     3121 gacccgcgcg aggcgcagat tcaggcctcg ctcaatcagg cgttccgcgg cctgttcctg
     3181 ggcggcatgg cggacgggct gcaaccggtc gaggcgctgg gcctgttcaa ggatttccgc
     3241 g
//
LOCUS       DASVLL010000279         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_285679,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000279 DASVLL010000000
VERSION     DASVLL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285679"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VF402_04685"
     CDS             complement(<1..843)
                     /locus_tag="VF402_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799623.1"
                     /translation="MGSSHTTSQGANRRWLRGALVAALCSGVSLTALSTAALAQDDPS
                     AAKPADDSTVVVVTGVRASQLKSVSIKRKAIAQVDAISSEDIGKLPDVTIADSLQRVG
                     GVQIRRDAGEGSSVNIRGLAQTITLFNGEQYLSSGNIGQAQANFLDVPSQLLNSVVVY
                     KSTDPATPLAGLSGTIDLQTHRPFNFKPGLTLTGAVEGQRGNITKDNDWNANMLASWR
                     NDRVGVLATVASSKSNLGDNYSGYGGGVFSENDWGQGIPQDYISPHGFEIYNRAIERK
                     RLGAN"
     gene            1318..2016
                     /locus_tag="VF402_04690"
     CDS             1318..2016
                     /locus_tag="VF402_04690"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEX7799624.1"
                     /translation="MTIWALNPQARLEVRRTGREGHPLLVVDNLLAAPEALVELAAQA
                     PFAPPQGTRYPGLNAPLPEAYLHAVTRPLRPVLQKEFGIPADNDLSLWGFFALATQGP
                     HSLAPIQTIPHQDSSDPMQLALVHYLCAETMGGTGFFRHRATGFEGVDAGRRPDYARI
                     AAAELAQSGGSGGHVRADTPRYEMTDSVDPAFNRLIVYRSHVLHAGLLDGIALSPDPR
                     AGRLTANSFLSPKR"
     gene            complement(2020..2175)
                     /locus_tag="VF402_04695"
     CDS             complement(2020..2175)
                     /locus_tag="VF402_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX7799625.1"
                     /translation="MLGTILIVVLILILLGALPAWPHSRSWGYGPSGGLGLLLVIIIV
                     LVLLGHI"
     gene            complement(2291..3028)
                     /locus_tag="VF402_04700"
     CDS             complement(2291..3028)
                     /locus_tag="VF402_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7799626.1"
                     /translation="MSFVDTVGALAADDPAPLYMQLQKILRDAIVTRLISPEAAIPPE
                     RDLADEFGVSRLTVRKAIDGLVEEGLITRRRGAGTFVAARVEKSFSKLSSFSEDMVSR
                     GRKPYSEWLNRSMGAVTPEESLSLGLSPGTLVYRFQRLRFADGQTMALEHATIPGYCL
                     PSLDSVNESLYAALQAAGHRPVRALQRLRAVAFSADQAHLLGVPAGDPGLFIERRGFL
                     ADGRAAEFTHSWYRGDAYDVVAELNGA"
BASE COUNT          527 a          981 c         1011 g          528 t
ORIGIN      
        1 gttggcgccg aggcgcttgc gctcgatggc gcggttgtag atttcgaagc cgtgcgggct
       61 gatatagtcc tgcgggatgc cctggcccca gtcgttttcc gagaagacgc cgccgccgta
      121 gccggaatag ttgtcgccca gattggactt ggacgaggcc accgtcgcca gcacgccgac
      181 gcggtcgttg cgccacgagg ccaacatgtt ggcgttccag tcgttgtcct tggtgatgtt
      241 gccgcgctgg ccctcgacgg cgccggtcag ggtcaggccc ggcttgaagt tgaacgggcg
      301 gtgggtctgc aaatcgatcg tgccggacag accggccaaa ggcgtggccg gatcggtcga
      361 tttatagacc acgacgctgt tcaaaagttg cgacggcacg tcgaggaagt tggcctgcgc
      421 ctgaccgata ttgcccgacg acaggtattg ctcgccgttg aacagggtga tggtctgcgc
      481 caggccgcgg atattgaccg acgagccttc gcccgcgtcg cggcggatct ggacgccgcc
      541 cacgcgttgc agcgagtcgg cgatggtgac gtcgggcagc ttgccgatgt cttccgagga
      601 gatggcgtcg acctgcgcga tggccttgcg cttgatgctg accgacttga gctgcgaagc
      661 gcgcacgccg gtgaccacga cgaccgtgct gtcgtcggcg ggtttggcgg cggacgggtc
      721 gtcctgcgcc agcgccgcgg ttgacagcgc tgtcagtgaa acgcccgaac agagcgcggc
      781 gaccagcgcg cccctgagcc agcgccggtt cgcgccctga ctcgttgtat gtgatgaccc
      841 cattgtactt gatgacatcg tctcgtccct attcctttga gttttgtcgt ttcgccggct
      901 ttcggcctga acaagcgccg cgccgacagt ttttttacgc gcccgacctg cgacctccct
      961 gagatgtcac aaggcggcga ccgccttcgc cggaaaacaa cgtggcggcg cgtctttatg
     1021 acaaggttgt catcatcgga cagcgtcagg cttcgcaaag cggcaaaaag atgtcaagaa
     1081 aaatgtccgc tttgttatga aagcggttcc accaccaaca atacggcgaa aaatggcgcg
     1141 aaaacgcaag gctttctatt ttatcgttta aatttgctgt tagcggtcac aaattcgccc
     1201 tgtcaaacca ctgtccacaa gacatgatct ttgtcttaag tgggcgacat gtgacgcgct
     1261 tgcatcaccc tgccgccgcc gttaagcttg gcagggacgc gcggcgcgga ggaccggatg
     1321 acgatatggg cgctgaatcc gcaggcgcgg ctcgaggtgc ggcgcaccgg gcgcgagggc
     1381 catccgctgc tggtggtcga caacctgctg gccgcgccgg aggcgctggt cgagctggcc
     1441 gcgcaggcgc cgttcgcgcc gccgcaaggc acgcgctatc cggggctgaa cgcgcccctg
     1501 ccggaagcat atctgcacgc cgtcacgcgc ccgctgcggc ccgtgctgca aaaagagttc
     1561 ggcattccgg cggataacga tctcagtcta tggggctttt tcgcgctggc gacgcaaggg
     1621 ccgcacagcc tggcgccgat ccagaccatt ccgcatcagg attccagcga tccgatgcaa
     1681 ctggcgctgg tgcattatct gtgcgcggag acgatgggcg gcaccggctt tttccgccat
     1741 cgcgccaccg gtttcgaggg cgtcgacgcg ggccgccgcc ccgactatgc gcgcatcgcg
     1801 gcggcggagc tggcgcagtc gggcgggtca ggcggccatg tccgggcgga cacgccgcgc
     1861 tatgagatga ccgacagcgt cgatccggcc ttcaaccgcc tgatcgtcta tcgcagccat
     1921 gtgctgcacg cgggtctgct ggacgggatc gcgctcagcc ccgacccgcg cgcgggacgg
     1981 ctgaccgcca acagcttcct gtcgccgaaa cgctgaccgc tatatgtgtc cgagcagcac
     2041 cagcacgatg atgatcacca gcagcaggcc cagaccgccg ctcggcccat agccccaact
     2101 gcggctgtgc ggccacgccg gcagcgcgcc gagcaggatc aggataagaa cgacgatcag
     2161 gattgtgccc agcatgacgc ggctccctta ttgtggcttc gtaacgcagc ctagggtctg
     2221 acggcgtaag ggcgatatgc gccgccgcgc ggcctttcat aaagggagcg tttcataatg
     2281 tccggaaacg tcaggcgccg ttcagctcgg ccaccacgtc ataggcgtcg ccgcgatacc
     2341 acgaatgggt gaattccgcc gcgcgcccgt cggcgagaaa gccgcgccgt tcgatgaaca
     2401 ggcccggatc gcccgccggc acgcccagga gatgcgcctg atcggcgctg aaggcgacgg
     2461 cgcgcaggcg ttgcagcgcg cgcaccgggc ggtggcccgc cgcctgcaag gccgcgtaga
     2521 gcgactcgtt cacgctgtcc agcgacggca ggcaatagcc gggaatggtg gcgtgttcca
     2581 gcgccatggt ctggccgtcg gcgaagcgca ggcgctggaa gcgatagacc agcgtgcccg
     2641 gcgacaggcc cagcgacagc gattcctccg gcgtgaccgc gcccatcgag cggttcagcc
     2701 attccgaata cggcttgcgc ccgcgcgaca ccatgtcctc ggagaaggag gacagcttgg
     2761 agaagctctt ttccacccgc gccgcgacga aggtgcccgc gccgcgccgg cgcgtgatca
     2821 gcccttcctc gaccagcccg tcgatcgcct tgcgcacggt caggcgcgac acgccgaatt
     2881 cgtcggccag gtcgcgctcg ggcgggatgg cggcttcggg gctgatcagg cgggtgacga
     2941 tggcgtcgcg cagaatcttc tgcaactgca tgtacagcgg cgcgggatcg tcggcggcga
     3001 gcgcgccgac cgtatcgaca aatgacatgc ccgccctccc cggcgaa
//
LOCUS       DASVLL010000280         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_447119,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000280 DASVLL010000000
VERSION     DASVLL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447119"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VF402_04705"
     CDS             <1..828
                     /locus_tag="VF402_04705"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX7799627.1"
                     /translation="EFQRFKTHPMMRGMFEGAKRLSYGARAISSGGVQSVPKLVFPGG
                     ALIGCSAGFVNVPRIKGSHNAIKSGILAADAAIQALQGQAPRVLEGYQAAYDASDIAR
                     ELKLVRNVKPLLSRLGTTLGTLVGGVEMWCTRLLGGFSPFGTLRHGKSDAHALKPAAE
                     FKPIAYPKPDGVLSFDKLSSVFLSSTNHEEDQPVHLKLKDPHIPIDINLPKYAEPAQR
                     YCPAGVYEIVGLDQHQPRLQINAQNCVHCKTCDIKDPTGNIVWTCPEGGGGPNYPNM"
     gene            941..2725
                     /locus_tag="VF402_04710"
     CDS             941..2725
                     /locus_tag="VF402_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7799628.1"
                     /translation="MRASSVFRVVLMLSAASALPAMAAPIMAAAPAELGAARSPYANF
                     LIGRYAIANGDVTTAAEAMNAAAAADPSSPDLRESAFLIGILNGDIERAAQEAPEMSK
                     GSETTQLMAPLAQAVVAARAGRKGDALRQVEAALKVRPQDRNAVLLRPYALALNGKWT
                     QAFDDSGDAALNGSDGGRLLVYLLKAERARLYELRGKTDKAEALYKDLCQPGAASYIF
                     GPDYAAFLERRGRADEAKTLWSGIAAQTGDAAAKAALARIAANGAPPALPDLKASMAQ
                     ALFVSSTIAYSERDSEMALASVRLSLYLDPTPDRVRIFLGQVEQALNDDDSADAVWAA
                     IGRDSPFYAEASLRRAGLRRDNGDSAGALNLLDQALSVAPDNLALVSEKAGLLHADFK
                     DRAALDALKARVAQAGDADFTWQTWFLEAMIYDSLDDWPDAETAIKKAQALNPGRPEI
                     LNFLGYGWISRGLHVQEGMDLVRQALNITPKSGAIIDSLGWGYYKLGDYDQALSFIEQ
                     AVQLEPADPEINEHLGDVYKAMGRNVEAGYEWGHVLTLKTSDKQAAEVRRKIDENAAA
                     TRLASTPAPAPVKAEMTALNDTPKSRRR"
BASE COUNT          474 a          963 c          940 g          427 t
ORIGIN      
        1 gaatttcagc gcttcaagac ccatccgatg atgcgcggca tgttcgaggg ggccaagcgc
       61 ctgtcctatg gcgcgcgcgc gatctcctcg ggcggcgtgc agtccgtgcc caagctcgtc
      121 ttccccggcg gcgcgctgat cggctgttcg gcgggcttcg tcaacgtgcc gcgcatcaag
      181 ggcagccaca acgcgatcaa gagcggcata ctggcggcgg acgcggcgat tcaggcgttg
      241 caggggcagg ccccgcgcgt gctggaaggc tatcaggccg cctacgacgc gtccgacatc
      301 gcgcgcgagc tgaagctggt gcgcaacgtc aagccgctgc tgtcgcggct cggcacgacg
      361 ctcggcacgc tggtcggcgg ggtcgagatg tggtgcacgc ggcttttggg cgggttctcg
      421 ccgttcggca cgctgcgcca cggcaagagc gacgcgcacg cgctcaagcc cgccgccgag
      481 ttcaaaccga tcgcctaccc caagccggac ggcgtgctgt cgttcgacaa gctgtcctcg
      541 gtctttttgt ccagcaccaa ccacgaggag gaccagccgg ttcacctcaa gctcaaagac
      601 ccgcacatcc cgatcgacat caacctgccc aaatacgccg aaccggcgca gcgctactgc
      661 ccggcggggg tgtacgagat cgtcggcctc gaccagcatc agccgcgcct gcagatcaac
      721 gcccagaact gcgtccactg caagacctgc gacatcaagg atccgaccgg caacatcgtc
      781 tggacctgcc cggaaggcgg cggcggcccc aactatccga atatgtaaga ccggcgcgtc
      841 atggctgacc tattgtcaac ttttgcgtga caggttttga ctttatagga ttgcagctta
      901 ctttcaggcc gtcctctgtg caaaggtcat ccgtgcttcc atgcgtgctt cttctgtttt
      961 tcgcgtcgtc ctgatgttga gcgccgcttc ggccctgccg gcgatggccg cgccgatcat
     1021 ggccgcggcc ccggcggagc tgggcgcggc gcgttcgccc tacgccaatt tcctgatcgg
     1081 acgctacgcc atcgccaacg gcgacgtgac cacggcggcc gaggcgatga acgccgccgc
     1141 cgccgccgat ccgtccagcc cggacctgcg cgagagcgcc ttcctgatcg gcatcctcaa
     1201 cggcgatatc gagcgcgcgg cgcaggaagc gccggagatg agcaaggggt cggagaccac
     1261 gcaactgatg gcgccgctgg cgcaggccgt cgtcgccgcg cgggcgggcc gcaagggcga
     1321 cgcgctccgg caggtcgagg ccgcgctcaa ggtgcgcccg caggaccgca acgccgtgct
     1381 gctgcgtccc tatgcgctgg ccctgaacgg caagtggacg caggcgttcg acgattcggg
     1441 cgacgccgcg ctgaacggca gcgacggcgg gcggctgctg gtctatctgc tcaaggccga
     1501 acgcgcgcgc ctctatgaac tgcgcggcaa gaccgacaag gccgaggcgc tctacaagga
     1561 tctgtgccag cccggcgcgg ccagctatat tttcggcccc gactacgccg ccttcctcga
     1621 acggcgcggg cgcgcggacg aggcgaaaac cctgtggagc ggcatcgccg cgcagaccgg
     1681 cgacgcggcg gcgaaggccg cgctggcgcg catcgccgcg aacggcgcgc cgcccgccct
     1741 gccggacctc aaggcgtcga tggcgcaggc tttattcgtg tcctcgacca tcgcctacag
     1801 cgagcgcgat tcggagatgg cgctggccag cgtgcgcctg tcgctctatc tcgacccgac
     1861 gcccgaccgc gtgcgcatct ttttagggca ggtcgagcag gcgctgaacg acgacgactc
     1921 cgccgacgcg gtctgggccg ccatcggccg ggactcgccc ttctacgccg aggcgtcgct
     1981 gcgccgggcg ggtctgcgcc gcgacaacgg cgacagcgcg ggcgccctga acctgctgga
     2041 tcaggcgctg agcgtggcgc ccgataatct ggctctggtc agcgaaaagg ccgggctgct
     2101 gcacgccgat ttcaaggacc gggcggcgct ggacgcgctc aaggcccgcg tcgcgcaggc
     2161 gggggacgcc gacttcacct ggcagacctg gtttctcgaa gcgatgatct acgattcgct
     2221 cgacgactgg ccggacgccg agacggcgat caagaaggcg caggcgctca atccgggccg
     2281 cccggaaatc ctgaacttcc tcggctacgg ctggatcagc cgcggcctgc acgtgcagga
     2341 gggcatggac ctcgtgcggc aggcgctcaa catcacgccg aagtcgggcg cgatcatcga
     2401 ttcgctcggc tggggctatt acaagctcgg cgactacgat caggccctga gcttcatcga
     2461 gcaggccgtg caactggagc ccgccgaccc ggagatcaac gagcatctcg gcgacgttta
     2521 caaggcgatg ggccgcaacg tcgaggccgg ttacgaatgg ggccatgtgc tgacgctgaa
     2581 aaccagcgac aagcaggccg ccgaggtgcg gcgcaagatc gacgagaacg ccgccgcgac
     2641 ccgcctggct tcgacgcccg cgcccgcgcc ggtgaaggcc gagatgacgg cgctcaacga
     2701 tacgcccaag tcgcgccgcc ggtaagatgg cgtccgtcga actggcgcgg gccaagatca
     2761 atctttacct gcatgtcgcc gcgcccgacg cgcgtggctg gcat
//
LOCUS       DASVLL010000281         4996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_501531,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000281 DASVLL010000000
VERSION     DASVLL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501531"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF402_04715"
     CDS             complement(<1..258)
                     /locus_tag="VF402_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799629.1"
                     /translation="MTIDRRAVVAGLTALGLLPAAAHGQTRRKEVLAFYYGWYATQSR
                     SDIVLHWPPQSPILDTPADGPYDSLDPAVIQRHAGQAKAAGL"
     gene            complement(248..643)
                     /locus_tag="VF402_04720"
     CDS             complement(248..643)
                     /locus_tag="VF402_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799630.1"
                     /translation="MAFKAPAKPLKRGRPLTAGKGMARKALTAAAATPEQRREQDKAA
                     LQKRTLRALDKARRLAAEQGVDLSDWEDAFLTGVSERVKTYGRAFRDPDKGAMNGTLS
                     LRQGVKLREIHKKAKGRMKDQTKDGPHDD"
     gene            complement(714..989)
                     /locus_tag="VF402_04725"
     CDS             complement(714..989)
                     /locus_tag="VF402_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEX7799631.1"
                     /translation="MADDAPSFGSDDVISGAAQTRLRTIIERIERLEEDKKGIMTDIK
                     EIYDEAKGEGFDVKILRKVVSLRKKDKVKREEEETILDLYLTAIGDR"
     gene            988..1179
                     /locus_tag="VF402_04730"
     CDS             988..1179
                     /locus_tag="VF402_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799632.1"
                     /translation="MLKVLLGGNGMGLTIANANRRLNAANQEAVDGFRAAATPGRTEK
                     PPEAGPQAVFRNAISGVKA"
     gene            complement(1176..1301)
                     /gene="ykgO"
                     /locus_tag="VF402_04735"
     CDS             complement(1176..1301)
                     /gene="ykgO"
                     /locus_tag="VF402_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX7799633.1"
                     /translation="MKVRSSLKSLKGRHRDCKIVRRKGVVYVINKTDPRFKAKQG"
     gene            1686..3026
                     /locus_tag="VF402_04740"
     CDS             1686..3026
                     /locus_tag="VF402_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX7799634.1"
                     /translation="MRARYLILCLLLTACASSAPQEALPPAPPPPPPPPVQPAPSEPA
                     PMPAAPVADTGAMSFDAWRTAFIAKAAAKGFDPLFVTNVLAGVQPLASVTAADSMQPE
                     FSRPISTYMRTAVSAARAAAARARLAANPDVPAIVRTYGVPSEILGGVWTMESDLGRV
                     QGDTDTVTALATLAWEGRRRALFEDELMAALTILQKGDIPRATLKGSWAGAMGQTQFE
                     PSSYLSMGVDGDHDGKVDIWNSDSDALASTANLLAKAGWKPVEEWAVEVVLPVGFDDY
                     LAETQKMTPAQWAALGVKRADGGVWTPAEADEQATLLLPSGAKGPAFLALPNHYVIRK
                     YNNSTAYALAVGLIADAIAGKPPLVTPWPVEPPLSLDQRRKSQMALKAAGFDPGAIDG
                     VIGVGTRQALRDWQRANHMAADGYLSADLADRFVATVDAASSTSAPAAPSAATP"
     gene            3128..4006
                     /gene="motA"
                     /locus_tag="VF402_04745"
     CDS             3128..4006
                     /gene="motA"
                     /locus_tag="VF402_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX7799635.1"
                     /translation="MFQIIGIVLLFGAVFGSYIMSGGNLAPVIEAAPHELMCIAGAAI
                     AATLIGNSLTTIKGIGGGFGKIFSGAKWKASDYRDLLSLLFLLTKTMKSKGVIALESH
                     IEKPHDSSIFNRFPKIVKDHFATDFICDTLRMMTMNLEDPHQVEDAMEKQLEKHHHEA
                     LAPSHALQNMADALPALGIVAAVLGVIKTMGSITEPPEILGAMIGGALVGTFMGVFLA
                     YGLVGPFSARLAAVVDEEGAFYKIIQSVLVAHLHGNAAQISVEIGRGSIPSESQPSFL
                     EMEEALNNIPAEAPAA"
     gene            4214..4414
                     /locus_tag="VF402_04750"
     CDS             4214..4414
                     /locus_tag="VF402_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799636.1"
                     /translation="MTSNIVRTALVAAAAVAALSVAACSKPAATTDSAAASSEAAPDA
                     AMTDSAMTADTSAVAASGAASS"
     gene            4558..4761
                     /locus_tag="VF402_04755"
     CDS             4558..4761
                     /locus_tag="VF402_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799637.1"
                     /translation="MTSKIVRIALVAAIAGAALSVAACSPKPAADEAAAASDAASDAA
                     SAAVDASAAASDAASAASEAASS"
BASE COUNT          814 a         1644 c         1642 g          896 t
ORIGIN      
        1 caggcccgcc gccttcgcct gtccggcgtg acgctggatc accgccggat cgaggctgtc
       61 gtaaggcccg tcggcgggcg tatcgaggat cggcgattgc ggcggccagt gcagcacgat
      121 gtcggagcgg ctctgggtcg cataccagcc gtaatagaag gccagcacct ccttgcggcg
      181 cgtctggcca tgcgccgcgg cggggagcag gcccagcgcg gtcaggcccg cgacgacggc
      241 gcgccggtca atcgtcatgg ggtccgtcct tcgtctggtc cttcatccgg cccttggcct
      301 tcttgtggat ttcgcgcagc ttcacgccct gacgcaggct cagggttccg ttcatcgcgc
      361 ccttgtcggg atcgcggaag gcgcgtccgt aggtcttcac ccgctccgaa acgccggtca
      421 ggaaggcgtc ctcccagtcg ctgagatcga cgccctgttc ggcggcgagg cggcgcgcct
      481 tgtcgagcgc gcgcagggtg cgtttttgca gggcggcctt gtcctgctcg cgcctctgct
      541 ccggcgtggc cgccgccgcc gtcagggcct tgcgcgccat acctttaccc gccgtgagcg
      601 gccgtccgcg tttcagcggc ttggcgggcg ccttaaacgc catgcgtcac acaggaaaag
      661 accgtcctca tacggttaag atgaggacgg tccgcacgat aagcaatctg tttttagcgg
      721 tcgccgatgg cggtcaggta caggtcgagg atggtttcct cctcctcgcg cttgaccttg
      781 tccttcttgc ggagcgaaac gaccttgcgc aggatcttga cgtcgaagcc ttcgcccttg
      841 gcctcgtcgt agatttcctt gatgtcggtc atgatgccct tcttgtcctc ttcgaggcgc
      901 tcgatgcgct cgatgatggt gcgaaggcgg gtctgggcgg cgccggaaat gacgtcgtcg
      961 gagccaaagg agggggcgtc gtcggccatg ctgaaagtcc tgctcggtgg aaacggaatg
     1021 ggtttaacca tagccaatgc gaatcgacgt ctcaacgccg cgaatcagga agctgtggat
     1081 ggttttcgcg cggcggccac gccgggacgc acggaaaaac cgcctgaggc cggaccccag
     1141 gcggttttca gaaacgcgat atcgggcgta aaggcttagc cttgcttggc cttgaagcgc
     1201 gggtccgtct tgttgatcac atagacgacg cccttgcggc gcacgatctt gcagtcgcga
     1261 tggcgaccct tgagcgactt gagcgagctg cggactttca ttggttttca cccggtcttt
     1321 aaaatggcgg ttcaggaagg gctttgggga caacctgtgc cggtcgcgcg cgtgggccaa
     1381 cttcgtaaga gcgcgggtac ttaggggaag ggcggcgaat tgtcaacggc tccgacgcca
     1441 taagttgacc ggggatgcgg gaaaaacgcc ggaaggcgcg gcgggcgccg caccggagcg
     1501 gcgatgaaag acattgtggc ttaccggcag ggcggcaggc aggcgttgaa atcggcccgc
     1561 gacggatttt agcccccggt aacggcccgg ataacggcga agtgagcttg caccaagtcg
     1621 gctccggctt ggaaagcgac cccattccgg tttagagtca agggattgcc agtccgggag
     1681 cttccatgcg cgcgcgctat ctcattctct gccttctgct gaccgcctgc gcgtcctcgg
     1741 cgccgcagga ggccctgccg cccgcgccgc ctccgcctcc gccgccgccc gtgcaaccgg
     1801 cgccgtccga acccgcgccg atgcccgccg cgcctgtggc cgatacgggc gcgatgagct
     1861 tcgacgcgtg gcgcacggcc ttcatcgcca aggcggcggc caagggtttc gatcccctgt
     1921 tcgtgaccaa cgtgctggcg ggcgtgcagc cgctggccag cgtgaccgcc gccgattcga
     1981 tgcagccgga gttctccagg ccgatctcga cctatatgcg cacggcggtc agcgccgcgc
     2041 gcgccgccgc cgcgcgggcg cggctggccg ccaatcccga cgtgcccgcc atcgtgcgga
     2101 cctacggcgt gccgtccgag attctgggcg gcgtctggac gatggagagc gatctcggcc
     2161 gcgtgcaggg cgacaccgac acggtgacgg cgctggcgac gctggcgtgg gaaggccgcc
     2221 gccgcgccct gttcgaggac gagctgatgg cggcgctgac catcctgcaa aagggcgata
     2281 ttccgcgcgc cacgctgaaa ggctcatggg cgggcgcgat gggccagacc cagttcgagc
     2341 cgtccagcta tctgtcgatg ggcgtcgacg gcgaccatga cggcaaggtc gatatctgga
     2401 attccgattc ggacgcgctg gcgtcgacgg ccaacctgct ggccaaggcg ggctggaagc
     2461 cggtcgagga atgggcggtc gaggtcgttc tgccggtcgg cttcgacgat tatctggccg
     2521 agacgcagaa gatgactccg gcccagtggg cggcgctggg cgtgaagcgc gccgacggcg
     2581 gcgtctggac gccggccgag gcggacgagc aggccacgct tctgctgccc tccggcgcga
     2641 agggaccggc ctttctggcc ctgccgaacc actatgtcat ccgcaagtac aacaattcga
     2701 ccgcctatgc gctggcggtc gggctgatcg ccgacgccat cgcgggcaag ccgcccctgg
     2761 tcacgccgtg gccggtcgag ccgccgctga gtctggacca gcgccggaag tcgcagatgg
     2821 cgctgaaggc ggcgggcttc gatccgggcg ccatcgacgg cgtgatcggc gtcggcacgc
     2881 ggcaggcgct gcgcgactgg caaagggcga atcacatggc cgccgacggc tatctgagcg
     2941 cggacctcgc cgaccgtttc gtggcgacgg tcgatgcggc gtcatccacg tccgcgcccg
     3001 ccgcgccatc cgccgcgacg ccgtaacaag gtaaaaaagt gcggttaacc ccgccggcct
     3061 gaaaccggct cttaatgatt aacggcgata gtggccgcct atcttctgag gcgggctgtc
     3121 gttcttcatg ttccagatta tcggtatcgt tcttcttttc ggcgcggttt tcggcagcta
     3181 catcatgtcc ggcggcaatc tggccccggt catcgaggcg gcgccgcacg aactgatgtg
     3241 catcgcgggc gccgccatcg ccgcgaccct gatcggcaac agcctgacca ccatcaaggg
     3301 aatcggcggc ggcttcggca agattttcag cggcgccaag tggaaggcgt ccgattaccg
     3361 cgatcttttg tcgcttttgt tcctgctgac caagacgatg aagtccaagg gcgtgatcgc
     3421 gctggaaagc cacatcgaga agccgcacga ttcgtcgatc ttcaaccgct tccccaagat
     3481 cgtgaaggac cacttcgcca ccgacttcat ctgcgacacg ctgcgcatga tgacgatgaa
     3541 cctcgaagac ccgcatcagg tcgaggacgc gatggagaag cagctcgaaa agcatcacca
     3601 cgaggcgctg gccccgtcgc acgccttgca gaacatggcc gacgcgctgc ccgcgctggg
     3661 catcgtcgcc gccgttctgg gcgtgatcaa gacgatgggc tcgatcaccg agccgccgga
     3721 aatcctgggc gccatgatcg gcggcgcgct ggtcggcacc ttcatgggcg tgttcctggc
     3781 ctacgggctg gtcgggccgt tctcggcgcg cctggccgcc gtggtcgacg aggagggcgc
     3841 cttctacaag atcatccagt cggtgctggt cgcgcacctg cacggcaacg cggcccagat
     3901 ctccgtcgag atcgggcgcg gctccatccc cagcgaatcg cagcccagct tcctggaaat
     3961 ggaagaggcg ctgaacaaca tcccggccga ggcgcccgcc gcctaaaaga cctcccgttc
     4021 gcccgccggg gctgggcgtt aaccattttg tggcgtaatt tcgtcatatc cggcgaaaaa
     4081 atccgggaat accggaaatg cgatcacagg tttgtgaaat agccccttgc gagactccgg
     4141 cgttttcggt atgagtgcga tgtgggcgta gggtctggtc cggcgctccg gttttagaac
     4201 aaggattgga accatgacct ccaatatcgt ccgcaccgcg ctcgtcgccg ccgccgctgt
     4261 ggccgccctg tccgtggccg cctgctcgaa gcccgccgcc acgaccgact ccgccgccgc
     4321 cagctccgaa gccgccccgg acgccgccat gaccgactcg gccatgaccg ccgacacctc
     4381 cgcggtggcc gcttcgggcg ccgcttcctc gtaatttcgc catttctggc gcgtccgcac
     4441 aaaagcgggg gcgctacgga aatccgccgg cggacaaccg tgcaggcggg gataaaccgg
     4501 atgcgaaact gaacctttcg catttgagtc tttctcttca aaaacaggat ttgatccatg
     4561 acctccaaga tcgttcgcat cgccctggtc gccgccatcg ccggcgccgc tctgtccgtc
     4621 gccgcctgct cgccgaagcc ggccgctgac gaagccgctg ccgcttcgga cgccgcttcg
     4681 gacgccgctt cggctgccgt cgacgcttcg gccgccgctt cggacgccgc ttcggccgct
     4741 tcggaagccg cttcgtccta agtctttcgc cctcgggttc atcccttggg gcaagagata
     4801 atgaaaaggg tcgccattgg cggccctttt ctgcattgtg ggccacgatg tccggagatt
     4861 cgccatgcgc cccgaaacgc gccgtaacat cctgatcctc agcgccggtc ttttgtgcgc
     4921 gacggccttg agcgcctgca agccgaaggc ggacgacagc gcaacggcgc agagcgcttc
     4981 gccgcaggac gcggcc
//
LOCUS       DASVLL010000282         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_69981, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000282 DASVLL010000000
VERSION     DASVLL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69981"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VF402_04760"
     CDS             complement(<1..1210)
                     /locus_tag="VF402_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7799638.1"
                     /translation="MSSPMQKFHGKSYFFRSLAAVSALALLSACATVPQSSTMTPLKA
                     ESLAANETLAAQPNGAWPARNWWRGFGDPQLDALIDEAFHNAPDLQAAQARIEKALAQ
                     SAQIKAATQADASLNVSDSVTKSSINMGTPSSVSFGSASFDPRSILPQGYWNLTRLSL
                     DANYDLDPWGKSRAAIHGARRAAEAAKVDADAARLTLAATITQAYVELSRLYDARDQL
                     NAMLSASDDRVALNQALLDHRLATKDAVLAEQDQQAQIKGQIASVDGAIRGQQNLIAA
                     LIGAGPDRGAQIARPHLAPADMGALPQNVTLDLLGRRPDIAAARLRVESQAAGIKYAK
                     ADFYPNVKLSAYWGIEALSRGGFNQLFQDGSDIGGIGPAFSLPLFHQGRLKVEYRSYE
                     ADYDSAVASYD"
     gene            1325..>1693
                     /locus_tag="VF402_04765"
     CDS             1325..>1693
                     /locus_tag="VF402_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7799639.1"
                     /translation="MEMLNEQVRETPSFKDQRRERIIQVARSVFFEVGYAGASMSLIS
                     QRLGGSKATLYAYFNSKEDLFAAIIREGCQDMAAIFRKHIGTNDLRSSLTQVAREMMS
                     LILSDWGNRTLQLIIEESPRN"
BASE COUNT          291 a          502 c          604 g          296 t
ORIGIN      
        1 ggtcgtagct ggcgaccgcc gagtcgtaat cggcctcata ggaccggtat tcgactttca
       61 ggcggccctg atggaacagg ggcaggctga aggccgggcc gatgccgccg atgtcggagc
      121 cgtcctggaa caactggttg aagccgccgc gcgacagggc ctcgatgccc caataggccg
      181 acagcttcac gttcgggtag aaatcggcct tggcgtactt gatcccggcg gcctgcgatt
      241 cgacgcgcag gcgggcggcg gcgatgtcgg ggcggcggcc cagaaggtcg agcgtgacgt
      301 tctgcggcag ggcgcccatg tcggcgggcg ccagatgcgg acgcgcgatc tgcgcgccgc
      361 ggtcgggacc ggcgccgatc agggcggcga tcaggttctg ctggccgcgg atggcgccgt
      421 cgaccgaggc gatctggccc ttgatctgcg cctgctggtc ctgttcggcc agcacggcgt
      481 ccttcgtggc gaggcggtgg tcgagcagcg cctgattgag cgccacgcgg tcgtcggacg
      541 cgctcagcat ggcgttcaac tgatcgcgcg cgtcgtagag gcggctcagt tcgacatagg
      601 cctgcgtgat cgtggcggcc agggtcaggc gcgccgcgtc ggcgtcgacc ttggcggcct
      661 cggcggcgcg ccgggcgccg tggatggcgg cgcggctctt gccccacggg tcgagatcgt
      721 aattggcgtc gagcgacagg cgcgtcaggt tccagtagcc ttgcggcagg atggagcgcg
      781 ggtcgaacga ggcggagccg aagctgaccg aggacggcgt gcccatattg atcgacgact
      841 tggtgacgct gtccgagacg ttcaggctgg cgtcggcctg cgtggcggcc ttgatctgcg
      901 cgctttgcgc cagcgccttt tcgatgcgcg cctgcgcggc ctgaaggtcg ggggcgttat
      961 ggaaggcttc gtcgatcagg gcgtcgagtt gcggatcgcc gaaaccccgc caccagttcc
     1021 gggccggcca ggcgccgttc ggctgcgcgg ccagggtttc attggcggcc aaagactcgg
     1081 ccttgagcgg cgtcatggtc gaggactgcg gcacagtggc gcaggcgctc agcagggcca
     1141 gcgcgctgac cgccgccaag ctcctgaaaa agtaggactt gccgtgaaat ttctgcatag
     1201 gggaggacat ggaggctgct ggtcctgaaa cacaaaaagg tgaaccgtac tgtacagttt
     1261 cgctccaatg cgctaatgtg cggcgcaagt caagctctaa ctgtacagta tggtacaaat
     1321 tcgtatggaa atgttaaacg aacaggtacg cgaaacgccg agcttcaaag atcagcggcg
     1381 cgagcgcatc attcaagtcg cccgaagcgt ttttttcgag gtcggctatg cgggcgccag
     1441 catgtcgctg atctcgcagc ggctgggcgg ctcgaaggcg acgctgtacg cctatttcaa
     1501 cagcaaggaa gacctgttcg cggcgatcat ccgcgagggc tgtcaggaca tggcggcgat
     1561 tttccgcaag catatcggca cgaacgatct acgctcgtcc ctgacgcagg tggcgcgcga
     1621 gatgatgagc ctgatcctgt ccgactgggg caaccgcacc ctgcaactga tcatcgagga
     1681 aagcccgcgc aac
//
LOCUS       DASVLL010000283         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_501604,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000283 DASVLL010000000
VERSION     DASVLL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501604"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..2050
                     /gene="leuA"
                     /locus_tag="VF402_04770"
     CDS             374..2050
                     /gene="leuA"
                     /locus_tag="VF402_04770"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7799640.1"
                     /translation="MTMLKDPASKYVPYVFPFDMSDRTWPAKRIEKAPRWLSTDLRDG
                     NQALADPMDVEKKMTFFNLLLKCGFKEIEVGFPSASQTEFDFVRKLIEENLVPDDVTI
                     QVLVQAREDLIRRTFESLRGAKKAIVHLYNATSPLFRRVVFNRTKEEVVELSVNGMRL
                     LREEAARQPDTDWSFEYSPETFTATEPEFALEVCEAVIAVIEPTPQKPLILNLPATVE
                     VAGPHVYADQIEWFCRNISKRDSVVISLHPHNDRGTGAAAAELGLLAGADRIEGCLFG
                     NGERTGNVDLVTLALNLYTHGVSPELDFSHMREVVRTVEYCNNIKVHPRHPYAGELVF
                     TAFSGSHQDAIRKGFAVQETRNDGVWDMPYLPIDPADLGESYEAVIRVNSQSGKGGIA
                     WVLEQTHGLNLPRRFQIDFSKKIQDITDASSRELSSAEIWDAFQSAYHLNGEQKYGLI
                     EYEQSGPVRSGAPRTFVGRISHDGVEASVIGHGNGLISSAVKALNDQYGLDLDVVDYA
                     EHALKQGSDGQAAAYVEARASDGRSVFGVGIDDDVATASVKAVLSAANAL"
     gene            2047..>2375
                     /locus_tag="VF402_04775"
     CDS             2047..>2375
                     /locus_tag="VF402_04775"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX7799641.1"
                     /translation="MRLRAALASFLLLIAAPALAATPRYSVQIVKTYPHDPAAFTEGL
                     FYLNDSLYESTGRNGQSWIRQVDLATGKPLRQVSIDRRYFGEGVAPWKGRIVSLTWRS
                     GVGFVWS"
BASE COUNT          460 a          815 c          704 g          396 t
ORIGIN      
        1 tgaaaggaaa cgaccctact ttccgacggt tcttggaacc caacacgaaa cggtccgtat
       61 ccttccgtcg tgactggggt ggctgccccg gtcacgaccg ttcatctcta gccaaaatga
      121 acacgcaaag catgagacaa ggcacgaaaa cagaaggtgg ccggagcgcc taagcccttc
      181 tcagcaatgc ttttggcaaa gccaaaaaac ctcagacggc ggatccgccg ctgtccgacg
      241 cacacgccgc cgctttcgtg tccttccgtg tttttcgtgg ccagaagacc tgccttcctc
      301 cccttcacaa ctctcgtcaa aacctcaatg acaattgacg ccgccgcgcg cgcgtgacac
      361 aagcgcggcc attatgacga tgctcaaaga ccccgcctca aagtacgtac cctacgtctt
      421 tcccttcgac atgtcggacc gcacatggcc cgcgaagcgc atcgaaaagg cgccgcgctg
      481 gctttcgacc gacctgcgcg atggcaatca ggcgctggcc gatccgatgg acgtcgagaa
      541 gaagatgacc ttcttcaacc tgctgctgaa gtgcgggttc aaggagatcg aggtcggctt
      601 cccgtcggcg tcgcagaccg agttcgactt cgtgcgcaag ctgatcgagg agaacctcgt
      661 ccccgacgac gtgacgattc aggtgctggt gcaggcgcgc gaggatctga tccggcgcac
      721 cttcgaatcc ttacgcggcg cgaagaaggc catcgtccac ctgtacaacg ccacctcgcc
      781 cctgttccgc cgcgtggtct tcaaccgcac gaaggaggag gtcgtcgagt tgtcggtgaa
      841 cggcatgcgc ctgctgcgcg aggaagccgc cagacagccc gacaccgact ggagcttcga
      901 gtacagcccc gaaaccttca ccgccaccga gccggaattc gcgctcgagg tctgcgaggc
      961 ggtgatcgcg gtgatcgaac cgacgccgca aaagccgctg atcctcaacc tgcccgccac
     1021 ggtcgaggtc gccggtccgc acgtttacgc cgaccagatt gaatggttct gccgcaacat
     1081 ctccaaacgc gacagcgtgg tcatctccct gcatccgcac aacgaccgcg gcaccggcgc
     1141 ggcggcggcg gagctgggcc tgctggccgg cgccgaccgc atcgaaggct gcctgttcgg
     1201 caacggcgag cgcaccggca atgtcgatct cgtcacgctg gcgctcaatc tctatacgca
     1261 cggcgtctcg ccggagctgg acttctcgca tatgcgcgag gtcgtgcgca cggtcgaata
     1321 ctgcaacaat atcaaggtgc atccgcgtca tccctatgcg ggcgaactgg tgttcaccgc
     1381 cttttccggc tcgcaccagg acgcgatccg caagggcttc gccgtgcagg agacgcgcaa
     1441 cgacggcgtc tgggacatgc cctacctgcc gatcgacccg gcggacctcg gcgaatcgta
     1501 cgaagcggtg atccgcgtca attcgcagtc gggcaagggc ggcatcgcct gggtgctgga
     1561 gcagacgcac ggcctgaacc tgccgcgccg cttccagatc gacttctcca agaagattca
     1621 ggacattacc gacgcctctt cgcgtgaatt gtcatcggcg gaaatctggg acgccttcca
     1681 gtcggcctac cacctgaacg gcgagcagaa atacggcctg atcgagtacg aacaaagcgg
     1741 cccggtgcgc tccggcgcgc cgcgcacctt cgtcggccgc atcagccatg acggcgtcga
     1801 agcctcggtg atcggccacg gcaacggcct gatctccagc gcggtcaagg cgctcaacga
     1861 ccagtacggc ctcgacctcg acgtggtgga ctacgccgaa cacgcgctga aacaaggcag
     1921 tgacggccag gccgccgcct atgtcgaggc gcgcgccagc gacggccgct ccgtcttcgg
     1981 cgtcggcatc gacgacgacg tggccaccgc ctcggtcaag gcggtgctga gcgccgccaa
     2041 cgccctatga ggctgcgcgc ggccctcgcc tcgttcctgc tgctgatcgc cgccccggct
     2101 ctggcggcca cgccgcgcta cagcgtccag atcgtcaaga cctacccgca cgatccggcg
     2161 gccttcaccg agggcctgtt ctacctgaac gacagcctgt acgaaagcac gggccgcaac
     2221 ggtcaatcct ggatacgtca ggtcgacctc gccaccggca agcccctgcg tcaggtcagc
     2281 atcgacagac gctatttcgg cgaaggcgtc gcgccgtgga agggccgcat cgtcagcctg
     2341 acctggcgat ccggcgtcgg cttcgtctgg tcgca
//
LOCUS       DASVLL010000284         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_231127,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000284 DASVLL010000000
VERSION     DASVLL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231127"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..509)
                     /gene="rpmE"
                     /locus_tag="VF402_04780"
     CDS             complement(285..509)
                     /gene="rpmE"
                     /locus_tag="VF402_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX7799642.1"
                     /translation="MKKEGHPDYHWITVTLTDGSSYKTRSTYGKEDAVLNLDIDPRTH
                     PAWTGGAGTLLDRGGRVSRFNNKFGGFLKK"
     gene            639..>2330
                     /locus_tag="VF402_04785"
     CDS             639..>2330
                     /locus_tag="VF402_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX7799643.1"
                     /translation="MPDSAPAAAKDRPSAGAELAAQIRHAAGGRQKTRNLKPLARLWP
                     YLMRQKRNLALMLVFLIVSSAASLGLTGAARFLVDKGFGSGDAARLDFWFAILGGVAI
                     VMALATAFRYFYITRLGERVVADLREDVFAHILRLDPAFFLSIRTGEVISRLTSDIQI
                     IETLVSSSISIALRNLVTFVGGLTLLMIVSPKLTLIVLALFPVVLVPLIVFGRAVGKL
                     TRKTQDNFAGAVGFASESLDALETVQAFVREAFTRRRFDAAVEGAYATSLQRIRARAT
                     MTALVISLVFGGVAMVLWLGAHDVFHHRMTTGALIQFVMLSVFTAGSVANLNETWGDL
                     QKAAGAMLRIDELLRSEPGVKAPPSPAQLPAPRGEIRFEHVRFAYPSRPEAVVLDDFS
                     LHVKPGETVALVGPSGAGKSSVFRLLLRYYDFTEGRIALDGVDIRACDPGAVRTRLSL
                     VAQDAALFSGSAADNIRFGREGATDEEVRAAAAKAQALGFIEALPQGFDTPLGERAKS
                     LSGGQKQRLSIARALVRDAPVLLLDEATSALDAENERLVQAALGEAMRDHTTLVIA"
BASE COUNT          341 a          761 c          807 g          421 t
ORIGIN      
        1 cgtcgagata gctgagcagc ccgtcatcga ccgcgatcca ctccgccgga tcgagcggtt
       61 tgacgccgat ggcgaacagc ttgtgcgaac cgtcgtacgg ggtgtgcatg tcaaaagctt
      121 agcaagaaag atcccccgcc accactcgct tcgtccgcgg tccccctccc ggcaaaccgg
      181 ggaggcatgg aaataaggga gcaacgggaa taaaaaatcc ggcccgcgcc tgtgacgcga
      241 gccggataaa ctcaaacaag ttttcgccgc ggcgcgttcg ccgcctactt cttgaggaag
      301 ccgccgaact tgttgttgaa gcgcgaaacg cggccgccgc ggtcgagcag cgtgccggcg
      361 ccgccggtcc aagccgggtg ggtgcgcggg tcgatgtcga ggttcaggac ggcgtcttcc
      421 ttgccgtagg tcgagcgcgt cttgtagctg gagccgtcgg tcagggtgac ggtgatccag
      481 tgataatcgg ggtggccttc ttttttcatc ttgcgatcct tgctacgtcg gccagcatga
      541 cgccgacgcc agaaaagggt tgagctaatc tttagcgaag cgcggctcat acaggaagcg
      601 gccttgccgc gcaagggctt cttctttaaa cacgcggcat gcccgactcc gcacccgccg
      661 ccgccaaaga ccgtccatcc gccggggccg aactggccgc ccagatccgc cacgcggcgg
      721 gcggccggca aaagacccgc aatctgaagc ctctggcgcg gttgtggccg tacctgatgc
      781 ggcagaagcg caatctggcg ctgatgctgg tgttcctgat cgtgtcgtcg gcggccagtc
      841 tcggcctgac cggcgcggcg cgctttctgg tcgataaggg attcggctcc ggcgatgcgg
      901 cgcggctcga cttctggttc gcgattctgg gcggcgtggc catcgtcatg gcgctggcga
      961 cggcgttccg ctacttctac atcacgcggc tgggcgagcg cgtggtggcc gacctgcgcg
     1021 aggacgtgtt cgcgcatatc ctgcggctcg atccggcctt tttcctgagc atccgcaccg
     1081 gcgaggtgat ctcgcgcctg accagcgaca tccagatcat cgagacgctg gtgtcgtcct
     1141 cgatctccat cgcgctgcgc aacctcgtca ccttcgtcgg cgggctgact ctgctgatga
     1201 tcgtcagccc caagctgacc ctgatcgtgc tggccctgtt tccggtcgtg ctggtcccgc
     1261 tgatcgtctt cggccgcgcg gtcggcaagc tgacgcgcaa gacgcaggac aatttcgccg
     1321 gcgcggtcgg cttcgcctcg gaatcgctcg acgcgctgga gacggtgcag gccttcgtgc
     1381 gcgaggcgtt cacgcgccgc cggttcgacg cggcggtgga gggcgcctat gcgacctcgc
     1441 tgcaacgcat ccgggcgcgc gccaccatga cggcgctggt gatctcgctg gtgttcggcg
     1501 gcgtcgccat ggtgctgtgg ctgggcgcgc acgacgtctt tcatcaccgc atgaccaccg
     1561 gcgcgctgat ccagttcgtc atgctgtcgg tgttcacggc ggggtcggtg gccaatctca
     1621 acgagacgtg gggcgacctg caaaaggcgg cgggggcgat gctgcgcatc gacgagctgc
     1681 tgcggtccga gccgggcgtc aaggcgccgc cgtctccggc ccagcttccg gccccgcgcg
     1741 gcgaaatccg cttcgagcat gtgcgcttcg cctatccgtc gcggcccgag gcggtggtgc
     1801 tggacgattt ttcgctgcat gtgaagccgg gcgagacggt ggcgctggtc gggccgtccg
     1861 gcgcgggcaa gtcgagcgtg ttccggcttt tgctgcgcta ttacgacttc accgagggcc
     1921 gcatcgcact ggacggcgtc gatatccgcg cctgcgatcc gggcgcggtg cgcacgcgcc
     1981 tgtcgctggt cgcgcaggac gcggccctgt tctccggctc ggcggcggac aatatccgtt
     2041 tcggccgcga gggcgccacg gacgaggagg tccgcgccgc cgccgccaag gcgcaggcgc
     2101 tgggcttcat cgaggccctg ccgcaaggtt tcgacacgcc tttgggcgag cgcgccaagt
     2161 cgctgtcggg cgggcagaag caacggcttt ccatcgcccg cgcgttggtg cgcgacgcgc
     2221 cggtgctgct gctggacgag gcgacctcgg cgctggacgc ggagaacgag cggctggtgc
     2281 aggcggcgct tggcgaggcc atgcgcgacc acaccacgct ggtcatcgcg
//
LOCUS       DASVLL010000285         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_124769,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000285 DASVLL010000000
VERSION     DASVLL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124769"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VF402_04790"
     CDS             <1..876
                     /locus_tag="VF402_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799644.1"
                     /translation="LVKRSTGVDFYPVNVHLNDKLFYELTYRATTDAKPVNTMYPARE
                     NLTQIAYEYRGEDAFRGGQVNPRHLLVFLKQCDAPASLQPRLRMLDGVWIIPPADAAD
                     LNLPKPQWSPIRSEVRFGWLDQGTCLLEGDWSRPEYDGVWTQSDQAGVVIPIQHVQFD
                     TPTPPKSLDLDLKARSRAPTTVSVLVNGVKAGELEIGRATARYTLRLPKSVMRADMLK
                     IRFVLKADEKIASKSLATVTLPSVAGRSAPKVKARPAASPGLGIKLMDLRLIDAADAA
                     QEAAQEAARAGLLNG"
     gene            1077..1550
                     /gene="greA"
                     /locus_tag="VF402_04795"
     CDS             1077..1550
                     /gene="greA"
                     /locus_tag="VF402_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7799645.1"
                     /translation="MEKVPMTVAGYQVLDDELKRLKSVERPSVIAAISEARSHGDLSE
                     NAEYHAAKERQGWIEGQIAEIEDKLSRAQVIDVSKLSGNQIKFGATVTVVDEDTEEEG
                     RYQVVGEHEADVKSGKISLSSPLSRAMIGKEVGDVVEVQTPGGVKAYEILKVEWL"
     gene            1569..>1986
                     /locus_tag="VF402_04800"
     CDS             1569..>1986
                     /locus_tag="VF402_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HEX7799646.1"
                     /translation="MPEKPIPEKLISEKLILWAVSDGRAGIENQALGLAEAVARLTPA
                     EIVVKRLRYRAPWDRLPTALKLWPDAMLRQDSDRIAAPWPDIWIGCGRASLPHGLRMR
                     ERSGGKTFVVQLQDPKTDLAPYDLVIAPEHDHLRGAN"
BASE COUNT          372 a          677 c          624 g          313 t
ORIGIN      
        1 ctggtcaagc gctcgaccgg cgtcgacttc tatccggtca acgtccacct gaacgacaag
       61 ctgttctacg agctgaccta ccgcgccacc accgacgcca agccggtcaa caccatgtat
      121 ccggcgcgcg agaacctgac ccagatcgcc tacgaatacc gcggcgagga cgccttccgc
      181 ggcggtcagg tcaatccccg gcacctgctg gtcttcctca agcagtgcga cgctccggcc
      241 agcctgcaac cgcgcctgcg catgctggac ggcgtatgga tcatcccgcc tgccgacgcc
      301 gccgacctga acctgcccaa gccgcaatgg tcgccgatcc gctccgaggt gcgcttcggc
      361 tggctggatc agggcacctg cctcctggaa ggcgactggt cgcgcccgga atatgacggc
      421 gtgtggacgc agagcgatca ggccggcgtg gtcatcccga tccagcacgt gcagttcgac
      481 accccgaccc cgcccaagtc gctggacctc gatctgaagg cgcgcagccg cgcccccacc
      541 acggtcagcg tgctggtcaa cggcgtcaag gcgggcgagc ttgagatcgg ccgcgccacc
      601 gcgcgctata cgctgcgcct gcccaaatcg gtcatgcgcg ccgacatgct gaaaatccgc
      661 ttcgttctca aggcggacga gaagatcgcg agcaagtcgc tggcgacggt gaccctgccg
      721 tccgtggccg gacggtcggc gccgaaggtg aaggcgcgtc ccgccgcctc gccgggcctc
      781 ggcatcaagc tgatggacct gcgcctgatc gacgccgctg acgccgccca ggaggccgcg
      841 caggaggccg ctcgggcggg attgctgaac ggctgatccc gccgccgccg gagaattgtg
      901 tttcaaaacg gccgttttcg ctttataagg agcgtccgcc cgcgttcgtg cgctgcgttc
      961 cgcagccgcc gcgcgggttc gccgtatcca aaacctgacc caagcggttg cagtcaccct
     1021 caaccgtcac ttgggttttt gtcattcctc agggtgaaat aaagtcttaa tcgcacatgg
     1081 aaaaagtgcc gatgaccgtc gcgggctatc aggttctcga cgatgaactg aagcgtctga
     1141 aatcggtcga gcgtcccagt gtgatcgccg ccatctccga ggcgcgctcg cacggcgacc
     1201 tgtccgaaaa cgcagaatat cacgccgcca aggaacgtca gggctggatc gagggccaga
     1261 tcgccgagat cgaggacaag ctcagccgcg cccaggtgat cgacgtctcc aagctatccg
     1321 gcaaccagat caagttcggc gccaccgtca ccgtggtcga cgaggacacc gaggaagaag
     1381 gccgctatca ggtggtcggc gaacacgagg ccgacgtgaa gtcgggcaag atctcgctgt
     1441 cctcgccttt gtcgcgcgcc atgatcggca aggaggtcgg cgacgtggtc gaggtccaga
     1501 cccccggcgg cgtcaaggcc tacgaaatcc tgaaagtcga gtggctctag gccggacgaa
     1561 ggcgcgtcat gccggaaaaa ccgatccccg aaaagctgat ctccgaaaag ctgattctct
     1621 gggcggtttc cgacggacgc gccggcattg aaaatcaggc gctggggctg gccgaggccg
     1681 tcgcgcgcct gacccccgcc gagatcgtcg tcaaacgcct gcgctatcgg gcgccgtggg
     1741 atcgcctgcc cacggcgctg aaactgtggc cggacgccat gctgcgccag gacagcgacc
     1801 ggatcgcggc gccgtggccg gatatctgga tcggctgcgg ccgcgccagc ctgccgcacg
     1861 gcttgcgcat gcgcgaacgc tccggcggca agaccttcgt cgtgcagttg caagacccca
     1921 aaacggacct tgcgccctac gatctggtca tcgcgccgga acacgaccac ttacgcggcg
     1981 ccaatg
//
LOCUS       DASVLL010000286         6886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_178525,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000286 DASVLL010000000
VERSION     DASVLL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178525"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF402_04805"
     CDS             complement(<1..284)
                     /locus_tag="VF402_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEX7799647.1"
                     /translation="MSSTTDKPVKKVVLAYSGGLDTSIILKWLQTEYGAEVVTFTADL
                     GQGEELEPARKKAELLGIKPENIFIEDLREEFVRDYVFPMFRANTVYEGQ"
     gene            complement(519..2507)
                     /locus_tag="VF402_04810"
     CDS             complement(519..2507)
                     /locus_tag="VF402_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX7799648.1"
                     /translation="MHGDTPSGPLTSDTGAATDGPASPKIDDVSGAESDAVSSHGHGS
                     EGFWALSVGAIGVVFGDIGTSPLYAMREALFHTREGTSADLAVKGVISLVFWALVMVV
                     TVKYVLFLMRADNKGEGGTLALMALAQKALGKYSPTVFFLGICGASLFYGDGIITPAV
                     SVLGAVEGLRDAPGVGHSLSHLIVPISAVILISLFFVQSKGTASVARLFGPITVLWFL
                     ALGALGLVHIFERPEILVALMPQYGVEFLFANGLTGFIILGSVFLAVTGAEALYADMG
                     HFGKKPIQYAWLAFVLPCLTLNYLGQGALTLHDPSARDNPFWRMVPDAFYWPTFALAT
                     MAAIIASQAVITGAYSMTQQAVQLGLLPRIDIKRTSETQAGQIFVPQINSMLMVGVLL
                     LVLLFQSTDRLTSMYGIAVTGAMMVDTLLAYVFLRHVWKWRRWQAWALLTPLGALDLV
                     FFGSNLLKIPQGAWLPLTLAAGLVLTMLTWATGTRTLTEKARRDSVMLADLIEMLKKR
                     PPHRVAGTAIFLTSDAEVAPVALMHNLKHNKVLHEKNIILTVRTANTPRVREADRVSI
                     EVLNEDFKKVTLAYGFMESPNLPRALGLCRKLGLKFDIMATSFFLGKRNVVPSANSGM
                     PLWQDKLFIFLMKNAANPTEFFHIPPGRVVELGTQVTV"
     gene            2663..3106
                     /locus_tag="VF402_04815"
     CDS             2663..3106
                     /locus_tag="VF402_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7799649.1"
                     /translation="MSDSQRFPVAAHTLAYLAHKNAYSREQAVSSSVLAASIPTNPVV
                     IRRMTTQLARAGLVATCSGVAGGAWLLRRPETIRLDEVLKAVNGCAHLGSAPPGAEGC
                     PVSQAIPKAVHGAMMQANAAAARSLSQITVADLLTHTRALPVEPA"
     gene            3171..3968
                     /locus_tag="VF402_04820"
     CDS             3171..3968
                     /locus_tag="VF402_04820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7799650.1"
                     /translation="MSRRLVLITGASSGIGMALARVYAGHGWDVALTARRGDRLSRLC
                     DEIRLRFGVEAHALPADLSEPGTPQKLHEGVLALGRTVDGLVNNAGYGLPGGFLGNPF
                     ETHQAMTQVMMLAPMELCHRVLPAMIEQKFGRVLNVASLAGYLPASPGDPLYSPIKTF
                     LIRFSQSLHLEMRDHGVHVSALCPGFTWSEFHDVSGQRAKLTRTIPEWAWMGADEVAR
                     EGYMAAEANRAACVPGAPNKTAASLLKVLPDDWVMELEARQRSRFLG"
     gene            complement(3960..4232)
                     /locus_tag="VF402_04825"
     CDS             complement(3960..4232)
                     /locus_tag="VF402_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEX7799651.1"
                     /translation="MARIVVRLTPRAAQERIDGWDADDHGRPVLKVRVRAAPIEGRAN
                     AALVALIAGALNCPKSRVILSGGETSRLKTLEIDGVDEAALRALSA"
     gene            complement(4225..4530)
                     /locus_tag="VF402_04830"
     CDS             complement(4225..4530)
                     /locus_tag="VF402_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX7799652.1"
                     /translation="MTDFIAYIVYGLLDFVIFCLIASAILSWLIAFNVINTRNRGVYM
                     IVELLDRITTPVLAPFRAFIPPVGGLDLSFLVGFLVIQAFKAYLLPPAFNTLRMLIG"
     gene            4747..4822
                     /locus_tag="VF402_04835"
     tRNA            4747..4822
                     /locus_tag="VF402_04835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4780..4782,aa:Ala,seq:cgc)
     gene            4987..5655
                     /locus_tag="VF402_04840"
     CDS             4987..5655
                     /locus_tag="VF402_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX7799653.1"
                     /translation="MTHRRLILATLAAALIAAPAVSPAWAQSGKNAFGGLISVPNFNA
                     ADKARIKAATDYLQTLSSASGRFEQTDSRGRVTRGGWWLQRPGKMRFEYDPPYAMVIV
                     SDGRTVSMWDPRLQTLDKAPLSETPLSLFLSRQIRLDQGVVVTAVSSNPQGFFLKARD
                     RRKQVEGSITLGFDQAANGALRLREWTIVDAQNRSTKVVLTSFKTGANPADLFVFNKP
                     QTKK"
     gene            5845..6645
                     /gene="xth"
                     /locus_tag="VF402_04845"
     CDS             5845..6645
                     /gene="xth"
                     /locus_tag="VF402_04845"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7799654.1"
                     /translation="MTFSLASWNINSVRLRIDQVARFAAEQAPDVLCLQEIKCQEHEF
                     PREAFAAMGYPHVMVTGQKGWHGVALASKQPFEIVGSPPFCRRGEARCQAVRLNGVEI
                     WNFYIPAGGDLPDRLLNDKFDHKLEFYERMSGALRARPKDQPLLICGDLNIAPGEYDV
                     WSHRQMLRVVSHTPPELEAFADFLDAGELHDPFRVAYPDPQKLFSWWSYRAADFRKSN
                     RGLRLDHILLNPGLRTQIKGDVQAVIHDTVREWDRPSDHAPIQTTFAL"
     gene            6642..>6886
                     /locus_tag="VF402_04850"
     CDS             6642..>6886
                     /locus_tag="VF402_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799655.1"
                     /translation="MTDLTESKLPAEILAKADFRFNEYAWRVADLPAVIEAAKNLNLL
                     NLGGQLQLRTEKAIGECYWVATDPCVHVPEDLPWDVRV"
BASE COUNT         1245 a         2281 c         2180 g         1180 t
ORIGIN      
        1 tactggccct catagaccgt attggcgcgg aacatcggga agacgtagtc gcgcacgaac
       61 tcctcgcgca ggtcttcgat gaagatgttt tccggcttga tgcccagaag ctcggccttt
      121 ttacgcgccg gctccagctc ctcgccctgc cccagatcgg cggtgaaggt cacgacctcc
      181 gccccgtact cggtttgcag ccacttcagg atgatcgagg tgtccagacc gcccgaatag
      241 gcgagaacga ccttcttgac gggcttgtcg gtggtggagg acatgagcgg aaacccttgt
      301 ttgcgaattc acgcgcgctt tagggtttta agccccgcga tgcaagggga aatgtcccct
      361 gtcttattcc ccaaaagctg aggtctgagg aattcgcgtc agggcgagcc gaaaagcttg
      421 cttttcgaga accggagcgg agcgtacgtc atgtacgtga gcaccggaag cgcagaaagg
      481 caggctttgc aggccgccct cacgcgaatg accagacctt acaccgtcac ctgcgtgcca
      541 agctcgacca cccgccccgg cgggatgtgg aagaactcgg tcggattggc ggcgttcttc
      601 atcaggaaga tgaacagctt gtcctgccac aaaggcatgc cggaattggc cgacggcacg
      661 acattgcgtt tgccgaggaa gaacgaggtc gccatgatgt cgaacttcag gcccagcttg
      721 cggcacaggc ccagagcgcg cggcagattc gggctttcca tgaagccgta ggccagggtg
      781 accttcttga aatcctcgtt cagcacctcg atcgacaccc ggtcggcttc gcggacgcgc
      841 ggcgtgttgg cggtcctgac ggtcaggatg atgttctttt cgtgcagcac cttgttgtgc
      901 ttgagattgt gcatcagggc caccggcgcg acctcggcgt cggaggtcag gaagatggcc
      961 gtgcccgcca cccggtgcgg cgggcgcttt ttcagcatct cgatcaggtc ggccagcatg
     1021 acggaatcgc gccgcgcctt ttcggtcagg gtgcgcgtgc cggtcgccca ggtcagcatg
     1081 gtcagcacca gccccgccgc cagcgtcagc ggcagccacg cgccctgcgg aatcttcagc
     1141 aggttcgagc cgaagaagac gaggtccagc gcccccagcg gcgtcagcag cgcccacgcc
     1201 tgccagcgcc gccacttcca gacatggcgc aggaagacat aggccagcag cgtgtcgacc
     1261 atcatggcgc cggtcacggc gatgccgtac atcgaggtca ggcggtcggt cgactggaac
     1321 agcagcacca gcagcagcac cccgaccatc agcatcgaat tgatctgcgg cacgaagatc
     1381 tggcccgcct gcgtctcgga ggtgcgcttg atgtcgatgc gcggcaggag gcccagttgc
     1441 accgcctgct gcgtcatcga ataggcgccg gtgatcacgg cctgcgaggc gatgatcgcc
     1501 gccatcgtcg ccagagcgaa ggtcggccag tagaaggcgt ccggcaccat gcgccagaac
     1561 ggattgtcgc gcgccgacgg atcgtgcagg gtcagcgccc cctgccccag atagttcagc
     1621 gtcaggcacg gcagcacgaa ggccagccac gcatactgga tcggcttctt gccgaaatgg
     1681 cccatatcgg cgtacagcgc ctcggccccc gtcacggcca gaaacaccga gccgaggatg
     1741 atgaagccgg tcaggccgtt ggcgaacagg aactcgacgc cgtattgcgg catcagggcg
     1801 accagaattt ccggccgctc gaaaatatgc acgaggccca acgcgcccag cgccaggaac
     1861 cacaagacgg tgatcggccc gaacaggcgc gcgacgctgg ccgtgccctt ggactgcacg
     1921 aagaacaggc tgatcagaat gaccgccgag atcggcacga tcaggtgcga cagggagtgg
     1981 ccgacgcccg gcgcgtcgcg caggccctcg accgcgccca gcaccgacac ggcgggcgtg
     2041 atgatgccgt cgccgtagaa caaggaggcg ccgcagatgc cgaggaagaa cacggtcggc
     2101 gaatacttgc ccagagcctt ctgcgccagc gccatcaggg ccagcgtgcc gccttcgccc
     2161 ttgttgtcgg cgcgcatcag gaacagcacg tacttcaccg tcaccaccat gaccagcgcc
     2221 cagaagacga gcgagatcac gcccttgacc gccagatcgg cgctggtgcc ttcgcgcgtg
     2281 tggaacaggg cttcgcgcat ggcgtacagc ggcgaggtgc cgatgtcgcc gaacaccacg
     2341 ccgatcgcgc cgacgctgag cgcccagaag ccttccgaac catgtccgtg actggagacg
     2401 gcgtcgcttt cggcgccgga aacgtcatcg atcttggggg aggcgggacc gtcggttgcg
     2461 gcgccggtgt cggatgtcag cgggccggaa ggggtgtccc catgcatgca tactctcctg
     2521 agggcgtgaa cgcgtcttcg gttgccctcg cttgaacccg gcgctcttac gcccatttat
     2581 agccttattc aaccgtcttg gccccagccg tgcgccaaac cgatttgacg cggggtcaaa
     2641 cctgcgtaac aatgacttga acatgtccga cagccaacgc tttcccgtcg ccgcgcatac
     2701 gctggcctat ctggcccaca agaacgccta ttcgcgcgaa caggcggtgt cctcgtccgt
     2761 gctggccgcc tcgattccga ccaatcccgt ggtgattcgc cgcatgacga cgcaactggc
     2821 cagggccggt ctggtcgcca cctgttccgg cgtggcgggc ggggcgtggc tgctgcgccg
     2881 cccggagacg atccggctcg acgaggtgct gaaggcggtc aacggctgcg cgcatctcgg
     2941 ctcggcgccg ccgggcgccg agggctgccc ggtcagtcag gccatcccga aggccgtgca
     3001 cggcgccatg atgcaggcca acgccgccgc cgcgcgcagc ctgtcgcaga tcaccgtcgc
     3061 cgatctcctg acgcacacgc gcgcgctgcc ggtggaaccg gcttaaatcg cttgcttttt
     3121 aacgcctgct cgtgttcgat ccgaaccaga gtcaccaccg ggggattgcc ttgtcgcgtc
     3181 gtcttgtcct gatcaccgga gcttcgtcag gcatcggcat ggccctggcg cgcgtctatg
     3241 ccggccacgg ctgggacgtg gcgctgacgg cgcggcgcgg cgaccggctg agccggttgt
     3301 gcgacgaaat ccgcctgcgt ttcggagtcg aggcgcacgc cctgcccgcc gacctcagcg
     3361 aacccggcac gccgcaaaag ctgcatgaag gcgtgctggc gctgggccgg acggtggacg
     3421 gcctcgtcaa caatgcgggc tacggcctgc ccggcggctt tctcggcaat ccgttcgaga
     3481 cgcatcaggc gatgacgcag gtgatgatgc tggcgccgat ggagctgtgc caccgcgtgc
     3541 tgcccgccat gatcgagcag aagttcggcc gcgtgctcaa cgtcgcctcg ctggccggtt
     3601 atctgcccgc ctcgcccggc gatccgctgt acagcccgat caagaccttc ctgatccgct
     3661 tttcgcagag cctgcatctg gagatgcgcg accacggcgt gcatgtcagc gccctgtgcc
     3721 ccggcttcac ctggtcggag ttccacgacg tcagcggcca gcgcgccaag ctgacgcgca
     3781 cgattcccga atgggcgtgg atgggcgccg acgaggtcgc gcgcgaaggc tatatggccg
     3841 ccgaggccaa ccgcgccgcc tgcgtgccgg gcgcgccgaa caagacggcg gcgtccctgc
     3901 tcaaggttct gcccgacgac tgggtgatgg agctggaagc gcgtcagcgc agccggtttc
     3961 taggctgaca aagcccgcag cgccgcttcg tccacgccgt cgatctccag cgttttaagg
     4021 cgcgacgtct cgccgccgct caggatcacg cgcgatttcg gacagttcag cgccccggcg
     4081 atcagcgcga ccagcgccgc gttggcccgg ccctcgatcg gcgcggcgcg cacgcggacc
     4141 ttcagcacgg gccggccatg atcgtcggca tcccagccgt cgatgcgctc ctgcgccgcc
     4201 ctgggcgtca gacggacgac gatcctagcc aatgagcata cgcagggtgt tgaaggcggg
     4261 cggcagcaga taggccttga acgcctgaat gaccagaaac ccgaccagaa agctcaggtc
     4321 cagcccgccg accggcggaa tgaacgcccg gaacggcgcc agcaccggcg tggtgatgcg
     4381 gtcgagcagc tcgacgatca tatagacgcc gcgattgcgc gtattgatga cgttgaacgc
     4441 gatcagccag ctcagaatcg ccgacgcgat caggcagaag atgacgaagt cgagcagtcc
     4501 gtaaacgata taggcgatga agtcggtcat atgtcctgcc gtggcgtccg gtaattcttc
     4561 cacatatagg ccattcgcca cgtctgacaa ggcgtctgac aggtcggggc gcgtccggcg
     4621 cggcgcgaag aaagaaaacg atccgggcgc ttgacaacgc cgccgcttcg cttaaataga
     4681 cggctcctta aacggctgac gcctcatgtc gccgcgaaaa ttcggcatga ggcgcgcgga
     4741 aggcgtgggg ctatagctca gttggtagag cgcctcgttc gcaatgagga ggccagcggt
     4801 tcgaatccgc ttagctccac cattccttcc ccccgccgtt tccaagtccc cccgtattcg
     4861 aagccgtcgt tcatccgccc ttcacgtcac ggccgcaaag cggcggcgcg ccgtcttttt
     4921 cgcgccctgc aattgccttg acgcccggtt aggctctaca caaccgatac gaagggacat
     4981 taccgcatga cgcaccgccg cctgatcctc gctacgctgg ccgccgccct gatcgcggcg
     5041 cccgccgtct cgccggcatg ggcgcaaagc ggtaagaacg cgttcggcgg cctgatttcc
     5101 gtgccgaact tcaacgccgc cgacaaggcg cgcatcaagg cggcgaccga ctatctgcaa
     5161 accctgtcca gcgcctccgg ccgcttcgag cagaccgaca gccggggccg cgtgacgcgc
     5221 ggcggctggt ggctgcaacg tccgggcaag atgcgcttcg aatacgatcc gccctatgcg
     5281 atggtgatcg tctccgacgg caggacggtc agcatgtggg atccgcgcct gcaaaccctc
     5341 gacaaggcgc cgctgtcgga aacgccgctg tccctgttcc tgtcgcgcca gatccggctc
     5401 gatcagggcg tggtggtcac ggcggtcagc tccaacccgc agggcttctt cctcaaggcg
     5461 cgcgaccgcc gcaagcaggt cgagggctcg atcacgctcg gcttcgatca ggccgccaac
     5521 ggcgccttgc gcctgcgcga atggacgatc gtcgacgcgc agaaccggtc caccaaggtg
     5581 gtgctgacca gcttcaagac gggcgccaat ccggccgatt tatttgtgtt caataagccg
     5641 cagactaaaa aataacgccg gactgatatt tccgattgcg cttttttgct gagcgtgtta
     5701 tttttcacac aatgggtcgc acggcggtcg actcagccgc ctgaagcgtt tcaggcttgc
     5761 gttttccctt ttttctcttc gtgttatgtg ccaaacctcg cccggccctc cgcaagaagg
     5821 ccgggctttt tgtagggtcg gggcatgacg ttctctctcg cttcgtggaa tatcaattcg
     5881 gtgcgcctgc gcatcgatca ggtcgcgcgc ttcgccgccg aacaggcgcc ggacgtgctg
     5941 tgcctgcagg aaatcaagtg tcaggaacac gaattcccgc gcgaggcgtt cgcggccatg
     6001 ggctatccgc acgtcatggt caccggccag aagggctggc acggcgtggc gctggcctcg
     6061 aagcagccgt tcgagatcgt cggttcgccg cccttctgcc gccgcggcga ggcgcgctgt
     6121 caggcggtgc ggctgaacgg ggtcgagatc tggaacttct acattccggc gggcggcgac
     6181 ctgcccgacc ggctgctcaa cgacaagttc gatcacaagc tggaatttta cgagcgcatg
     6241 agcggcgcac tgcgcgcgcg gccgaaagac cagccgctgc tgatctgcgg cgacctcaac
     6301 atcgcgccgg gcgaatatga cgtgtggagc caccgccaga tgctgcgcgt ggtcagccac
     6361 accccgccgg agctggaggc gttcgccgac ttcctcgacg cgggcgagct gcatgacccg
     6421 tttcgcgtcg cctatcccga tccgcagaag ctgttttcat ggtggagcta tcgcgccgcc
     6481 gacttccgca aatccaatcg cggcctgcgc ctcgaccata tcctgctcaa tccgggtttg
     6541 cgcacgcaga tcaagggcga tgtgcaggcc gtcatccatg acacggtgcg cgaatgggac
     6601 cgcccctccg accacgcgcc gatccagacc acgttcgcgc tatgaccgac ctgaccgaaa
     6661 gcaagctgcc cgccgagatt ctggcgaaag ccgatttccg cttcaacgaa tatgcgtggc
     6721 gcgtcgccga tctgcccgcc gtgatcgaag cggcgaaaaa cctgaacctg ctgaacctcg
     6781 gcggccagtt gcagttgcgc accgaaaagg ccatcggcga atgctactgg gtcgccaccg
     6841 atccgtgcgt ccacgtcccc gaagacctgc cgtgggacgt gcgcgt
//
LOCUS       DASVLL010000287         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_447847,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000287 DASVLL010000000
VERSION     DASVLL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447847"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VF402_04855"
     CDS             <1..372
                     /locus_tag="VF402_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7799656.1"
                     /translation="ATGLTRRVRIEDGRLTRVIFVAPIHKTLPPRDWLLERFGDATLS
                     DDDRAALLIGRLPGVEDKGRTICACRSVGEKTIMAAISDGAGSVEAIGEATTAGTACG
                     SCKGELKQCLLKHAKETSHAA"
     gene            458..1309
                     /gene="cobA"
                     /locus_tag="VF402_04860"
     CDS             458..1309
                     /gene="cobA"
                     /locus_tag="VF402_04860"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX7799657.1"
                     /translation="MAGGVAPHPIAFAALGSRPLPVNGERGITGAPRPGFVTLCGAGP
                     GGADHLTLGALKALQGAQAVLYDALVSDEILALLPQRCVKVCVGKRGDRLSVPQDRTN
                     ALMVRLARRGLAVVRLKGGDPSVFGRVEEERLWLDRHGIAHTTLPGVTAASAAAAQFA
                     FPLTHRGEARSVTFLTGRGRDGSIDFRDGLLADPSASLCFYMSSHNAAHIQASLLRAG
                     RSPATPVMAVENAGRETARAVRGTLSDLAAMTAGAAWDGPVLLGVGAAFAYARLETRL
                     APAAASA"
     gene            1321..>1860
                     /locus_tag="VF402_04865"
     CDS             1321..>1860
                     /locus_tag="VF402_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX7799658.1"
                     /translation="MRRLLLLALLIATPAQAELHPYLQKATVRFQPLPPAQARPVNPD
                     VRYVGGYDLQARGTDFLTGLSDLQLFPDGGGLRAEAISDYGAIALFDLRPDAEGGFQD
                     SELSVDPLRDADGEPYYDKRFGDAEDIAYDPRTGAHYVSFEGYQRVMRYTDWHGRGET
                     LRLSGLPPFPSNEGMEGLTF"
BASE COUNT          270 a          650 c          661 g          279 t
ORIGIN      
        1 gcgacaggat tgacgcggcg ggtgcgcatc gaggacggac ggctgacgcg ggtgatcttc
       61 gtggccccga tccacaagac gctgccgccg cgcgactggc tgctggagcg gttcggggac
      121 gcgaccttga gcgacgacga ccgcgccgcc ctgctgatcg ggcgcctgcc cggcgtcgag
      181 gacaagggcc gcaccatctg cgcctgccgc agcgtcggcg aaaaaaccat catggccgcc
      241 ataagcgacg gcgcgggcag cgtcgaagcc atcggcgagg ccaccaccgc cggcacggcc
      301 tgcggctcgt gcaagggcga gttgaaacag tgcctgctga aacacgcgaa ggagacgtcc
      361 catgccgcat aaagatggcg ttcggccaca gccccctctc ccctttcaag gggagagggt
      421 tggggtgagg ggttcctcgc cggcaaaggc gacgcctgtg gcgggcggag tcgcccctca
      481 cccgatcgcc ttcgctgcgc tcggctctcg tcctctcccc gtaaacgggg agagggggat
      541 cactggtgcg cctcgaccgg gtttcgtcac cctctgcggc gccggccccg gcggggcgga
      601 tcatctgacg ctcggcgcgc tcaaggcgct gcaaggcgcg caggcggtgc tgtacgacgc
      661 gctggtcagc gacgagatcc tcgccctgtt gccgcaacgc tgcgtcaagg tgtgcgtggg
      721 caagcgcggc gacaggctgt cggttccgca ggaccgcacc aacgcgctga tggtccggct
      781 ggcgcggcgc gggctggcgg tggtgcggct caaaggcggc gatccgtcgg tgttcggccg
      841 cgtcgaggag gagcgcctct ggctcgaccg ccacgggatc gcgcacacaa ccctgcccgg
      901 cgtgacggcg gcctcggcgg cggcggcgca gttcgcgttt ccgctcaccc atcgcggcga
      961 ggcgcgctcg gtgaccttcc tgaccggacg cgggcgcgac ggttccatcg atttccgcga
     1021 cgggctgctg gccgatccgt cggccagcct gtgcttctac atgtcctcgc acaacgccgc
     1081 tcacatccag gcgtcgctgc tgcgcgccgg acgcagcccc gccaccccgg tcatggcggt
     1141 cgagaacgcc ggacgcgaga cggcgcgcgc ggtgcgcggg actttaagcg atctggccgc
     1201 gatgacagcc ggtgcggcgt gggacggacc ggtcctgctc ggcgtcggcg ccgccttcgc
     1261 ctatgcgcgg ctggagacgc ggcttgcccc ggcggcggcc agcgcgtaaa tctggcggcc
     1321 atgcgccgcc ttctgctgct cgccctcctg atcgccacgc ccgcgcaggc ggaactgcat
     1381 ccgtacctgc aaaaggcgac ggtgcgattt cagccgctgc cgcccgcgca ggccagaccc
     1441 gtcaacccgg acgtgcgcta tgtcggcggc tatgacttgc aggcgcgcgg cacggatttc
     1501 ctgaccggcc tgtcggacct gcaacttttc cccgacggcg gcggcctgcg cgccgaggcg
     1561 atcagcgatt acggcgccat cgccctgttc gacctcaggc ccgacgccga aggcggcttt
     1621 caggacagcg aactcagcgt cgaccccttg cgtgacgcgg acggcgaacc ctattacgat
     1681 aagcggttcg gcgacgccga ggacatcgcc tacgatccgc gcaccggcgc gcattacgtg
     1741 tcgttcgagg gctatcagcg cgtgatgcgc tatacggact ggcatggccg gggcgagacg
     1801 ctgcgcctga gcggcctgcc gccgtttccg tcgaacgagg gcatggaggg gctgaccttc
//
LOCUS       DASVLL010000288         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_178811,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000288 DASVLL010000000
VERSION     DASVLL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178811"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VF402_04870"
     CDS             <1..1181
                     /locus_tag="VF402_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX7799659.1"
                     /translation="MTARAALDQDARDRAAREGRERNFSRDLNDWTRRTGDSAARLAK
                     LQKDEEQARAALDAAQAAPHQFESKRLSLVDSLATAEKRLNEARDALNAAEAARRDAD
                     IDVRAREQEASAAREERAGAQARLEAIQQRAHEIEAVILDQTGGTPDDLGKRLKEEAI
                     ATPSDASGAEALLSGLEKERDQLGAVNLRAEEEAAEYEGRLETLSKERADLITAIAKL
                     RDGIDELNAEGRERLLAAFEVINAHFKTLFVTLFGGGSAELRLIESDDPLEAGLEIFA
                     CPPGKRLSSMSLMSGGEQALTATALIFGVFLANPAPVCVLDEVDAPLDDANVDRYCRL
                     LAEMRERTRTRFIAITHNPVTMSRMDRLFGVTMAERGVSQLVSVDLSQAEAMVAEHVA
                     "
     gene            1432..1728
                     /locus_tag="VF402_04875"
     CDS             1432..1728
                     /locus_tag="VF402_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX7799660.1"
                     /translation="MTEDPLNDPSAEEKLKDLDQRLNAIDARERSEKSDMAAEVGANK
                     GYQALGELMGGILGGLGLGWLSDHYLHTLPWGMIVGAILGMAAAVYAIVKTSQK"
     gene            1821..2582
                     /locus_tag="VF402_04880"
     CDS             1821..2582
                     /locus_tag="VF402_04880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7799661.1"
                     /translation="MADPLHQFKIETVVPGPAFDVGGLHIDLSITNSVLAMMIAVGLT
                     LLFFVLSTARASIIPGRMQVMAEGVFGLVDDLTDSIIGHDGRQFFPYVFTLFLFVLSC
                     NLLGMFTFFTATSQVAVTGTLAAMTILLVIGVGVIRHGLGFFRMFLPSGVPWYLSWLI
                     VIIEVISFLVRPVTLCLRLFGNMVGGHIVMKVFAGFIIALAALGIGGWIGGLLSLSTI
                     VALTSLEFLVAYLQAFVFAVLACVYLSDVVNIGHH"
     gene            2623..>2768
                     /locus_tag="VF402_04885"
     CDS             2623..>2768
                     /locus_tag="VF402_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX7799662.1"
                     /translation="MDLQSAQAIAHGLQFIGAGLATLGMIGAGIGLGILFGNYYVGAL
                     RNPSA"
BASE COUNT          463 a          913 c          874 g          518 t
ORIGIN      
        1 cgatgacggc gcgcgccgcg ctggatcagg acgcgcgcga ccgcgccgcc cgcgaaggcc
       61 gcgagcgcaa tttcagccgc gacctgaacg actggacgcg ccgcaccggc gattccgccg
      121 cgcgcctcgc caagctgcaa aaggatgagg aacaggcgcg cgccgcgctc gacgccgcgc
      181 aggccgcgcc gcatcagttc gagtcgaagc gtctcagcct cgtcgattcg ctggccaccg
      241 ccgaaaagcg gctgaacgag gcgcgcgacg ccctgaacgc cgccgaagcg gcccgccgcg
      301 acgccgacat cgacgtgcgc gcccgcgagc aggaagcgtc cgccgcgcgc gaggaacgcg
      361 ccggggcgca ggcgcggctg gaggcgattc agcagcgcgc gcacgaaatc gaggccgtga
      421 tcctcgacca gaccggcggg acgcccgacg acctgggcaa gcggctgaag gaggaggcca
      481 tcgccacccc gtccgacgcc tccggcgccg aggcgctgct gtccgggctg gagaaggagc
      541 gcgaccagtt gggcgcggtc aatctgcgcg ccgaggagga ggccgccgaa tacgaggggc
      601 gcctcgaaac cctgtcgaag gagcgcgccg acctgatcac cgccatcgcc aaactgcgcg
      661 acggcatcga tgagctgaac gccgagggac gcgagcgcct gctggccgcg ttcgaggtga
      721 tcaacgcaca tttcaagacc ctgttcgtca ccctgttcgg cggcggctcg gcggagttgc
      781 ggctgatcga atcggacgat ccgctcgaag cgggcctgga aatcttcgcc tgtccgccgg
      841 gcaagcgcct gtcaagcatg agcctgatgt cgggcggcga gcaggccctg accgccacgg
      901 cgctgatctt cggcgtgttc ctggccaatc cggcgccggt ctgcgtgctg gacgaggtcg
      961 acgcgccgct cgacgacgcc aatgtcgacc gctattgccg cctgctggcc gagatgcgcg
     1021 aacgcacccg cacgcgcttc atcgccatca cccacaaccc ggtcaccatg tcgcgcatgg
     1081 accgcctgtt cggcgtcacc atggccgaac gcggcgtctc gcaactggtc agcgtcgacc
     1141 tgtcgcaggc cgaggcgatg gtggcggagc atgtggctta agggtttggg gtcacggaag
     1201 ccgcgaaaag cagaacctgc gcggaagcct gattgcgcgc tgttataaac ttgcagaaaa
     1261 acggcgcgtc gaaaatctta tgacagcgct gtttttattt tcttttcggc ttatttcccg
     1321 cagcgttgtt tttgcgccat cctattgctt gacctttgca tgtctcttga gtaggttccg
     1381 cgccgttcga ggccggaaca acgccgttgt tccgtctgtt gggtgctgaa cgtgacagaa
     1441 gaccccctaa acgacccttc ggcggaggaa aagctgaagg atctggatca gaggctgaac
     1501 gccatcgatg cgcgcgagcg cagcgaaaag tccgatatgg ccgccgaggt cggcgccaat
     1561 aagggctatc aggcgctggg cgagctgatg ggcggcattc tgggcggtct gggtctgggc
     1621 tggctcagcg atcattatct ccacacgctt ccgtggggca tgatcgttgg cgcgatcctc
     1681 ggcatggcgg cggcggtcta tgcgatcgtc aaaaccagcc agaaataagg cgtccggcgg
     1741 atttcgcgcc tgaaattcga tatttatggc ggtccgccga tcccgacggg ccgttcttgt
     1801 taagaggttg attgaggctg atggccgatc cgttacacca gttcaaaatc gagaccgtcg
     1861 tccccggccc ggccttcgac gtgggcggtc tgcatatcga cctctccatc accaattcgg
     1921 tgctggccat gatgatcgcg gtcggcctga ccctcctctt cttcgtcctg tccaccgcgc
     1981 gcgccagcat cattccgggc cgcatgcagg tgatggccga aggcgtgttc gggctggtcg
     2041 acgacctgac cgattcgatc atcggccacg acggccgcca gttctttcct tacgtcttca
     2101 ccctgttcct gttcgtcctg agctgcaacc tgctcggcat gttcaccttc ttcaccgcca
     2161 cctcccaggt cgcggtcacc ggcacgctgg ccgcgatgac catcctgctg gtcatcggcg
     2221 tcggcgtgat ccgccacggc ctcggcttct tccggatgtt cctgccgtcg ggcgtgccgt
     2281 ggtacctgtc gtggctgatc gtgatcatcg aagtgatctc gttcctggtc cgtccggtca
     2341 cgctgtgcct gcggttgttc ggcaacatgg tcggcggcca catcgtcatg aaggtgttcg
     2401 ccggcttcat catcgcgctg gcggcgctgg ggatcggcgg ctggatcggc ggccttctgt
     2461 cgctcagcac catcgtggcg ctcacctcgc tcgaattcct ggtcgcgtac ctgcaagcct
     2521 tcgtcttcgc ggtgctggcc tgtgtctatc tcagtgacgt ggtcaatatc ggccaccact
     2581 aaaaaccttt ttaaaatcaa taccctcacg gagctaaatc ctatggacct tcaatccgct
     2641 caagccatcg cccacggcct gcaattcatc ggcgcgggcc tcgccacgct cggcatgatc
     2701 ggcgccggca tcggcctcgg catcctgttc ggcaactact atgtcggcgc cctgcgcaac
     2761 ccgtcggc
//
LOCUS       DASVLL010000289         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_392786,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000289 DASVLL010000000
VERSION     DASVLL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392786"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1630
                     /locus_tag="VF402_04890"
     CDS             86..1630
                     /locus_tag="VF402_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEX7799663.1"
                     /translation="MTPTLTLICALPTALTFEIGNGTCFESPAPHEIFVNGQSALTTA
                     RNVFTLRGLQSGADHRIEVRSGGKSLKTTARTRPLSVVLKPEDFGAKGDGVSDDTSAL
                     QAALSAAPPDAIIRLERSYLTGPLFLKSRTHLDIAKGAKLLGLRDIEKWPILPGLLYD
                     EQGKETAWLGSWEGRPQGCHAALLNILSATDVSVFGKGLIDGQSGFDTWWAHPKTQQA
                     GSWRPRLIFAVDSDNLTFEGFTLANSPSWNLHPLHCRKVTAACLNITAPADSPNTDGF
                     DPESCEDVVGAGIHFTVGDDCIAIKSGKIEMARKDLRPTRGVRISNCWMQDGHGGVTL
                     GSEMAAGIYGVVVRDCRFTGTDRGVRVKTRRGRGKDAIVKGAWFDNIEMNEVGTAFVV
                     NSFYDVDADAKTDYVRSRAPQPVSDLTPWLGGFKLTNIRCDKVRNAVVYLLGLPEQPI
                     EGVEIDGLYARFDPDAKPAPPDMAASIPPERHRGIYIVNARHVRLRHIDIEGQTGDKI
                     HLENVS"
     gene            1627..2793
                     /locus_tag="VF402_04895"
     CDS             1627..2793
                     /locus_tag="VF402_04895"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7799664.1"
                     /translation="MIVLPNVPDSVRAAPVKRPFTLSGGALAALSRFGAQVPVFVESY
                     LRVWAPYRDVWNYEDGCVWTGALALYDAIGDRALLDFVHAEVGLRVMPDGRIPGFHPS
                     EFNIDGVQAGRVLEPLWRLTGEARFRAAMDAQFAQLLTHPRTRSGNYWHKNIYPHQVW
                     LDGLYMAQPFQTQYARVTDRPYLFEDTVAQFRFAAGALKDPRTGLYRHGWDESRLERW
                     ADPDTGRSSCVWGRAVGWWVMALADVYDLSQGLDAALRAEIAALLLDAATALLSVRSD
                     AGLWYQVADQGARDGNYEEASASLMIAYALMKGARLGVLPRDHGAAGLTALRAACGRF
                     VSPDRLSGVCGVAGLGNTPYRDGSYAYYLSEKITPNDPKGVAALMMALAEGLQI"
     gene            2804..3640
                     /locus_tag="VF402_04900"
     CDS             2804..3640
                     /locus_tag="VF402_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEX7799665.1"
                     /translation="MPSIAEQFVAARRAARALPGYPGQIPEKLSDSYAIQEQAIRLWN
                     RPVAGWKIGRLAPDKQTLHGAERLAGPVFDTLCKPYDGDVEIPVFTGGFAAVEAEYVF
                     RVGADADPDKTDYSEAEALALVDGLFAGIEMAGSPLATINALGPTVVVSDFGNNFGLI
                     LGPRLMALTPDMPVAGFQDNALTEWRAVTEIDGRVVGEGGLFTMPGGPLKAIGWLAGH
                     LAARGRPLKAGQLITSGATTGIHDILAGQAASVRFSQPGCADVSLDLHTVALDPQRPE
                     LA"
BASE COUNT          661 a         1307 c         1275 g          631 t
ORIGIN      
        1 cagggggcaa gacacccctc cgtcgttcgc tgtgcgaacg ccacctcccc acatcgtggg
       61 gaggagagaa gatgaggcat cggccatgac ccctaccctg accctgatct gcgccctgcc
      121 gaccgcgttg accttcgaga tcggcaacgg aacctgcttc gaaagcccgg cgccgcatga
      181 gatcttcgtc aacgggcaat ccgccctgac cacggcgcgc aacgtcttca ccctgcgcgg
      241 cctgcaaagc ggcgccgacc accgcatcga ggtccgcagc ggcggcaaga gcctgaaaac
      301 cacggcgcgc acgcggcccc tgagcgtcgt cctcaaaccg gaagatttcg gcgcgaaagg
      361 cgacggcgtc agcgacgaca caagcgcgct gcaagccgcc ctgtccgccg cgccgccgga
      421 cgctatcatc cggctggaaa ggtcgtatct gaccggcccg ttatttctca aaagccggac
      481 acatctcgac atcgccaaag gcgcgaaact gctcggcctg cgcgacattg aaaaatggcc
      541 gatcctgccg ggcctgctct acgacgaaca gggtaaggaa accgcctggc tcggctcatg
      601 ggagggccgg ccgcagggct gtcacgccgc cctgctcaac atcctgagcg ccacggacgt
      661 ctccgtcttt ggcaaagggc tgatcgatgg ccagtcgggc ttcgacacct ggtgggcgca
      721 tccgaaaacg caacaggccg gaagctggcg cccgcgcctg atcttcgcgg tcgacagcga
      781 caacctgacc ttcgagggct tcaccctggc caattcgccg tcgtggaacc tgcaccccct
      841 gcattgccgc aaggtgacgg cggcctgcct caacatcacc gcccccgccg acagcccgaa
      901 caccgacggc ttcgacccgg aaagctgtga ggacgtggtc ggcgcgggca tccatttcac
      961 ggtcggcgac gactgcatcg ccatcaagtc gggcaagatc gagatggcga ggaaagacct
     1021 gcgcccgacg cgcggcgtgc gcatctcgaa ctgctggatg caggacggac acggcggcgt
     1081 cacgctcggc tcggagatgg cggcggggat ttacggcgtc gtggtgcgcg actgccgctt
     1141 caccggcacg gaccgcggcg tgcgcgtcaa gacgcggcgc ggacgcggca aggacgccat
     1201 cgtcaagggc gcctggttcg acaatatcga gatgaacgaa gtgggcacgg ccttcgtggt
     1261 caacagcttc tatgacgtgg acgccgacgc caaaaccgac tatgtccgct cgcgcgcgcc
     1321 gcagccggtc agcgacctga ccccgtggct gggcggcttc aagctgacca acatccgctg
     1381 cgacaaggtg cgcaacgcgg tcgtctatct gctgggcctg cccgaacagc cgatcgaggg
     1441 cgtcgagatc gacgggcttt acgcgcgctt cgacccggac gccaaaccgg cgccgcccga
     1501 catggccgcc tcgatcccgc cggagcggca tcgcggaatc tacatcgtca acgcccgcca
     1561 tgtccgcctg cgtcacatcg acatcgaagg ccagacgggc gacaaaatcc atctggagaa
     1621 cgtgtcatga tcgtgctgcc gaacgtgccg gacagcgtca gggccgcgcc ggtgaagcgt
     1681 cccttcacct taagcggcgg cgcgctggcc gcgctgtcgc gcttcggcgc gcaggttccg
     1741 gtgttcgtcg aatcctacct gcgcgtctgg gcgccgtatc gcgatgtctg gaactatgag
     1801 gacggctgcg tgtggaccgg cgcgctggcg ctttacgacg cgatcgggga cagggcgctg
     1861 ctcgatttcg tccacgccga ggtcggcctg cgcgtcatgc cggacggccg gattcccggc
     1921 tttcacccgt ccgagttcaa tatcgacggc gtgcaggccg ggcgcgttct ggagccgctg
     1981 tggcggctga caggcgaggc gcgtttccgg gcggcgatgg acgcgcagtt cgcccagctt
     2041 ctgacccatc cgcgcacccg atccggcaat tactggcaca agaacatcta tccgcatcag
     2101 gtctggctgg acggcctcta catggcccag ccgttccaga cgcagtatgc gcgcgtcacg
     2161 gaccgtccct atctgttcga ggatacggtg gcgcagttcc gcttcgccgc cggggcgctg
     2221 aaagacccgc gcaccggcct ttaccgccac ggctgggacg aaagccgcct tgaacgctgg
     2281 gccgacccgg acacgggccg ctcgtcctgc gtctgggggc gggccgtggg ctggtgggtc
     2341 atggcgctgg ccgatgtcta tgacctgtcg cagggcctcg acgccgccct gcgcgccgag
     2401 atcgccgccc tgctgctgga cgccgccaca gcgctgctca gcgtgcgctc ggacgccgga
     2461 ttatggtatc aggtcgccga tcagggcgcg cgcgacggca attacgagga ggcttcggcc
     2521 tcgctgatga tcgcctatgc gctgatgaag ggcgcgcgcc tcggcgtgct gcccagggat
     2581 cacggcgcgg cgggcctgac cgctttgcgg gctgcgtgcg ggcgtttcgt ctcgccggac
     2641 cgcctgagcg gcgtttgcgg ggttgcgggc ctcggcaata cgccgtatcg cgacggtagt
     2701 tatgcctatt acctgtcgga aaagatcacg ccgaacgatc cgaaaggcgt ggcggcgctg
     2761 atgatggcgc tggccgaagg tttgcagatt taaggatttc cccatgccct ccatcgccga
     2821 acagttcgtc gccgcgcgca gagccgccag ggctttgccc ggctatccgg gacagattcc
     2881 cgaaaagctg agcgattcct acgctattca ggagcaggcg atccggctct ggaaccgccc
     2941 ggtcgcgggc tggaagatcg gccgcctcgc ccccgacaag cagacgctgc acggcgcgga
     3001 gcggctggcc gggccggtgt tcgacacgtt gtgcaagccc tatgacggcg acgtcgaaat
     3061 ccccgtcttc accggcggtt tcgccgccgt cgaggccgaa tacgtctttc gcgtcggcgc
     3121 ggacgccgat cctgacaaga cggactacag cgaggccgag gcgctggctc tggtcgacgg
     3181 cctgttcgcc gggatcgaga tggccggatc gccgctggcc accatcaacg cgctgggccc
     3241 cacggtcgtc gtctccgact tcggcaacaa tttcggcctg atcctggggc cgcgcctgat
     3301 ggcgctgacg ccggacatgc cggtcgccgg tttccaggac aacgccctga ccgaatggcg
     3361 cgccgtcacc gaaatcgacg gccgggtcgt cggcgaaggc ggccttttca ccatgcccgg
     3421 cgggccgctc aaggccatag gctggctggc cgggcatctg gcggcgcgcg gccgcccgct
     3481 gaaggccggg caactgatca cctccggcgc aaccaccggc attcacgaca tccttgccgg
     3541 acaggccgcg tccgtgcgct tctctcagcc gggatgcgcg gacgtaagcc ttgacttgca
     3601 tacggtcgcg ctcgatccgc aacgcccgga actggcctag gtcggatcga catttgcttg
     3661 gcttgagcgg atcgcgctct gacggaaggc ggatataacc gcggatggct cgctcagatt
     3721 aagaagggat ttaaccacgg aaaacacgga tgatggcctt tggccatctt cacggacaag
     3781 gaaaggcgcg ggactttccg cgcgctcatc cttgtccgtg tacgccgcgc aagcggcggt
     3841 ccgtgaagat ggccgaaggt catcgtccgt ggtt
//
LOCUS       DASVLL010000290         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_448030,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000290 DASVLL010000000
VERSION     DASVLL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448030"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF402_04905"
     CDS             <1..366
                     /locus_tag="VF402_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7799666.1"
                     /translation="EVLKAKKPSVKMIAIEPEASAILSGGEPGPHKIQGLGAGFVPAI
                     LDRNVIDGVETVSNDAAFAMARKSASLEGLPVGISSGAALEVAFRLAARDEYAGKTIV
                     AIIPSFAERYLSTALFENL"
     gene            533..1825
                     /locus_tag="VF402_04910"
     CDS             533..1825
                     /locus_tag="VF402_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX7799667.1"
                     /translation="MAGMIAGMSTPNASAAAYAESDEDADGAAVAEAPAAEVAPKRQA
                     RLTLTRRLADVVCLPESRINAFERAVTADLLVEMLAEADAAVRARVARRLSLLSEAPW
                     SLVRLLIVDEPEVARPLIEDCESLSDADLNYCVKRGAEAHHLLLAGRKDLPPLIAEGL
                     IETGRAPVIEVLLKNMRASLSQPAVEAAAALSREHPHLIPLLLKRPEMRPSSAYVLFW
                     WANAECRRLILTRFAVNREIMQDMASDVFAMMAAEKWQDPLARKALQFIERRQRNREA
                     LKKSPYDSLEAAIAEAAKTGMTRVLAEEISYLAGLKPATGAKLMRDPGGEGLAVMCKA
                     TGLSRKALRALWKALRRPMRDAGGQIHPDLANVLVTYDVLATDRAQTVLRYWNWSLSS
                     ALTHAMLRTIASGVDVDYESLSVPERAAMLAFAQDLQA"
     gene            1960..2388
                     /locus_tag="VF402_04915"
     CDS             1960..2388
                     /locus_tag="VF402_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX7799668.1"
                     /translation="MENKTDTLDMTADIVSAFVGNNSVPASELPALIQSVYQALNAIS
                     SGEETKVEAPKEPAVSVKKSISSDYIVCLEDGRKFKSLKRHLRTKYGMSPEDYRAKWS
                     LPKDYPMVAPNYAKARSDLAKQMGLGQGGRQVARKSRAKV"
     gene            2621..>3126
                     /locus_tag="VF402_04920"
     CDS             2621..>3126
                     /locus_tag="VF402_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7799669.1"
                     /translation="MLAALHAHEAVFRYWKSLRRNGRLPSRADFDPSAVKLRLPTISL
                     IDVLDAAPNERAACFRQRLAGTELFAAYGAEITGKTLDAIYTADEAQYWAEHLSFVVA
                     QRKPNVGLHSMAWRGAPALSLFWLRLPLASDGENVDMILGHDALIGKIDLTPALTEAH
                     LLGQVRTRL"
BASE COUNT          556 a         1017 c         1011 g          542 t
ORIGIN      
        1 gaggtgctca aggccaagaa gccgtcggtg aagatgatcg ccatcgagcc ggaagcctcg
       61 gccatcctgt cgggcggcga gcccggcccg cacaagattc aggggctggg cgccggtttc
      121 gtgcccgcca tcctcgaccg caacgtgatc gacggcgtcg agaccgtctc caacgacgcc
      181 gccttcgcca tggcgcgcaa gtcggccagc ctggaagggc tgccggtcgg catttcgtcg
      241 ggcgccgcgc tggaagtcgc cttccgcctg gccgcgcgcg atgaatatgc gggcaagacc
      301 atcgtggcga tcatcccgtc cttcgcggaa cgctacctgt cgaccgcgct gttcgagaac
      361 ctgtaaaggc gttacggcgg ggcctatgtg aagaggcccc tcccaccgcc ttcggcggtc
      421 cccgtttacg gggaaggcag cgccgccgga ggcggtgacg gaagggggac ttcgcagacc
      481 gaggctgtgg cttgcggatt cccgttaacc gaaactcacc aaagattaac ggatggcggg
      541 catgatcgcg ggcatgtcga ccccgaacgc gtccgccgcc gcctatgccg aatccgatga
      601 ggatgcggac ggcgccgccg tggcggaggc gcccgccgcc gaggttgcgc cgaagcggca
      661 ggcgcggctg accctgacgc gccgtctggc cgacgtggtc tgcctgcccg aaagccgcat
      721 caacgccttc gagcgcgcgg tgaccgccga cctgctggtc gagatgctgg ccgaagccga
      781 cgcggctgtg cgcgcgcgcg tcgcgcgccg gctcagcctc ctgtcggagg cgccgtggtc
      841 gctggtgcgc ctgctgatcg tcgacgagcc ggaggtggcg cgcccgctga tcgaggactg
      901 cgaatcgctg agcgacgctg atctcaacta ttgcgtcaaa cgcggcgccg aggcgcatca
      961 cctgctgctg gccgggcgca aggatctgcc gcccctgatc gccgagggcc tgatcgagac
     1021 cggccgcgcg ccggtcatcg aagtcctgct gaaaaacatg cgcgccagcc tgtcccagcc
     1081 cgccgtcgag gcggcggcgg cgctgagccg cgagcatccg cacctgattc cgctgctgct
     1141 gaaacgcccg gagatgcgcc cgtcctcggc ctatgtgctg ttctggtggg ccaacgccga
     1201 atgccgccgc ctgatcctga cgcgcttcgc cgtcaatcgc gagatcatgc aggacatggc
     1261 cagcgacgtc ttcgccatga tggccgccga aaagtggcag gatccgctgg cgcgcaaggc
     1321 gctgcaattc atcgaacggc gccagcgcaa ccgcgaggcg ttgaagaaaa gcccctacga
     1381 ctcgctggag gccgcgatcg ccgaggcggc caagaccggc atgacgcgcg tgctggccga
     1441 ggaaatctcc tatctggccg ggttgaagcc cgccaccggc gccaagctga tgcgcgatcc
     1501 gggcggcgag gggctggccg tgatgtgcaa ggcgaccggc ctgtcgcgca aggcgctgcg
     1561 ggcgctgtgg aaggcgctgc gccgcccgat gcgcgacgcg ggcgggcaga ttcaccccga
     1621 cctcgccaat gtgctggtca cctacgacgt gctggccacc gaccgcgccc agacggtgct
     1681 gcgctactgg aactggtcgc tgtcctcggc cctgacccac gccatgctgc gcaccatcgc
     1741 ttcgggcgtc gacgtcgatt acgaatcgct gagcgtgccc gaacgcgccg ccatgctggc
     1801 tttcgcgcag gatttgcagg cctgaaagcc gttctttttc ttggctctca agtaaatatc
     1861 ggcctctata ggccgatgaa gtgttttttt gagatcacat cttgaacact agcggcaatt
     1921 agcttagtta aagcgaaagg ttctcaaggg atacgcgtaa tggaaaataa aaccgatacc
     1981 ctggatatga cggcggacat cgtttccgct tttgtcggca acaattcagt gcccgccagc
     2041 gagttgcccg cactgattca aagcgtatat caggcgctca acgccatatc ctccggtgag
     2101 gaaaccaagg tcgaggcgcc caaggagcct gcggtttctg tcaagaagtc gatttcttct
     2161 gattacatcg tctgtcttga agacggacgc aagttcaagt cgctgaagcg ccacctgcgc
     2221 accaagtacg gcatgagccc cgaagattat cgcgccaagt ggagcctgcc gaaggattac
     2281 ccgatggtcg cgccgaacta cgccaaggcg cgttcggacc tggccaagca gatgggcctc
     2341 ggtcagggcg ggcgtcaggt cgcgcgcaag agccgcgcca aggtctgagc ctcggcttta
     2401 atactatgcg aaaaagctcc ggtcccacgg ccggagcttt ttctttaccg ccgattcctc
     2461 cataagaatc cacagcttcg gcgccgggtg taaagccgcg ttaacccttt tttgcttgcc
     2521 aaggaatcgc ctgttaagag aaggtgaatg cgggtgattc ggtaaaatat ttacgctggg
     2581 gcagtggcgc ttattcccta tggctggttt ggcttccgat atgctggcgg cgcttcacgc
     2641 gcatgaagcc gttttccgct actggaaaag cctgcgccgg aacggccgcc tgccgtcgcg
     2701 cgccgatttc gacccgtcgg cggtcaagct gcgcctgccg accatcagcc tgatcgacgt
     2761 gctggacgcc gcgccgaacg agcgcgcggc ctgtttccgg caaagactgg cgggcaccga
     2821 actgttcgcc gcctatggcg cggagatcac cggcaagacg ctggacgcca tctatacggc
     2881 ggacgaggcg caatactggg ccgagcatct gagcttcgtc gtcgcgcagc gcaagcccaa
     2941 tgtcggcctg cattcgatgg cgtggcgcgg cgcgcccgcg ctcagcctgt tctggctgcg
     3001 cctgccgctg gccagcgacg gcgaaaacgt cgacatgatc ctgggccacg acgccctgat
     3061 cggcaagatc gacctgacgc ccgccctgac cgaggcccat ctgcttggcc aggtccgaac
     3121 gcgcct
//
LOCUS       DASVLL010000291         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_339571,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000291 DASVLL010000000
VERSION     DASVLL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339571"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1211
                     /locus_tag="VF402_04925"
     CDS             57..1211
                     /locus_tag="VF402_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX7799670.1"
                     /translation="MNPVFAALPTSIFETMSLAAKANGAINLGQGFPETDGFPQVREA
                     AARALIEQSNQYAPMRGLPVLRQAVGDFYARTQGIALDPDREVVVTSGATEAICAALL
                     ALITPGDEVVVFEPMYDAYVPLIERAGGVARIVQLSPPRWRFSDDDLKAAFNARTRAV
                     LLTTPNNPTTTCLDRAQLDLLAGYCERFDAYVIADEVWEQVTFGQKHLSVLHLPILRN
                     RSVKIGSAGKIFSLTGWKVGFVCAVPELADQIARAHQFITFATPPALQHGVAYGLNLP
                     QAAFDAARAELARQRACLSEALTAQGFAVLPGEGTYFLNIDLRASGLAGSGLDIAQRL
                     VRQNGVATIPLQAFCPTGRDLPVLRLCFAKSPATLEDGARRLASARGAQV"
     gene            complement(1188..2075)
                     /locus_tag="VF402_04930"
     CDS             complement(1188..2075)
                     /locus_tag="VF402_04930"
                     /inference="COORDINATES: protein motif:HMM:NF022472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HEX7799671.1"
                     /translation="MFKRLLLLVLAAGLLTSVAGARPVHRPLPAERVTDDLGVPVAEA
                     LRAEARGRRVLVVFDIDNTLLTTPQDLGGDNWYVWKRSQDDRRAIGDLLRDDGALLAL
                     SDMRPTQPDMAALIARLQAKGVAIYALSARGPDLRGATERVLSAAGVDLSRAPECGPP
                     LCIKRGRLGAAQIHAAGVRVGWPVTGPFRPVTVSDGVMLVAGQDKGLMLDLLLHSLPT
                     RYDEVWFVDDTPENIDHVRHAAPHIRARLHPYAYSRLWPDAAAFMQDKARQDKAEQGM
                     AAVRAGVCAAIVSDLCAAR"
     gene            complement(2121..>3046)
                     /locus_tag="VF402_04935"
     CDS             complement(2121..>3046)
                     /locus_tag="VF402_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX7799672.1"
                     /translation="AILKDWPVVQQARLGDRRVADYLKGFDSGQPTPVLEASGQTEGR
                     FAYASDMQEFNFSRRNKTVSGGIEDIFAVTGQTNAPYIYIQSVPTPQFLPGFAEQNRH
                     PFLPPAIWPRIWISNATRAQTHNDNADNIACVAAGRRRFTLFPPEQVGNLYIGPMDHT
                     PSGRAISLASLEAPDFERFPKLAEALGHAQVAELEPGDALYIPKYWWHHVQSLSPFNV
                     LVNYWWGNSASTADNPMTPFLAALLALKDVSPADKRYWKAMFDHYIFQTGEDPVAHIP
                     QGHQGGLGPHNPRLRAEILKALQGLVNAGAR"
BASE COUNT          469 a         1000 c         1080 g          497 t
ORIGIN      
        1 atcgactcgt tcgccaccga aggcggcatc gacctgtgga aggtgtcctc gaccctatga
       61 accccgtctt cgccgccctg ccgaccagca ttttcgagac catgtcgctg gcggcgaagg
      121 cgaacggggc catcaatctg gggcagggct ttcccgaaac ggacggcttc ccgcaggtgc
      181 gcgaggccgc cgcgcgggcg ctgatcgaac agtcgaacca gtacgcgccg atgcgcggtc
      241 tgccggtgct gcgccaggcg gtcggcgact tttatgcgcg cacgcaaggg atcgcgctcg
      301 atcccgaccg cgaggtggtc gtcacctccg gcgccaccga ggcgatctgc gccgcgcttc
      361 tggccctgat cacgccgggc gacgaggtgg tggtgttcga gccgatgtac gacgcctatg
      421 tgccgctgat cgaacgggcg ggcggcgtgg cgcgcatcgt gcaactgtcg ccgccgcgct
      481 ggcgcttcag cgacgacgac ctgaaggcgg cgttcaacgc gcgcacgcgc gccgtgctgc
      541 tgaccacgcc gaacaatccg accacgacct gcctcgaccg cgcgcagctc gacctgctgg
      601 ccgggtattg cgagcggttc gacgcctatg tcatcgccga cgaggtgtgg gagcaggtga
      661 cgttcggcca gaaacatctg agcgtgctgc atctgcctat cttgcgcaac cgcagcgtca
      721 agatcggctc ggcgggcaag atcttctcgc tgaccggctg gaaggtcggc tttgtgtgcg
      781 ccgtgcctga gctggccgat cagatcgccc gcgcgcatca gttcatcacc ttcgcgacgc
      841 cgcccgcctt gcagcatggc gtggcctatg ggctgaacct gccgcaggcc gcgttcgacg
      901 cggcgcgggc cgagctggcg cggcagcgcg cctgcctgag cgaagcgctg accgcgcagg
      961 gtttcgcggt cctgccgggc gaggggactt actttctcaa tatcgacctg cgcgcgtcgg
     1021 gtctggcagg ctccggtctc gatatcgcgc agcgtctggt gcggcaaaac ggcgtggcga
     1081 cgatcccgtt gcaggcgttt tgcccgacgg gacgcgacct gcccgtgctg cggctgtgct
     1141 ttgcgaaatc gccggcgacg cttgaggacg gcgcgcggcg tctggcctca gcgcgcggcg
     1201 cacaggtctg agacgatcgc cgcgcacacg cccgcgcgga cggccgccat gccctgttcc
     1261 gccttgtcct gccgcgcctt gtcctgcatg aaggcggcgg cgtctggcca caggcggctg
     1321 taggcgtaag gatgcaggcg ggcgcggata tgcggcgccg catgacgcac atggtcgatg
     1381 ttttcgggcg tgtcgtcgac gaaccagacc tcgtcgtagc gcgtcggcag gctgtgcagc
     1441 aaaaggtcga gcatcagccc cttgtcctgc ccggcgacca gcatcacgcc gtccgacacc
     1501 gtgaccgggc ggaacggccc cgtgacgggc cagcccacgc gcacgccggc ggcgtgaatc
     1561 tgcgccgcgc ccagcctgcc gcgctttatg cataagggcg ggccgcattc cggcgcgcgc
     1621 gacaggtcga ccccggcggc gctcagcacg cgctccgtgg cgccgcgcag gtccgggccg
     1681 cgcgcgctca gcgcatagat ggcgacgcct ttcgcctgca agcgcgcgat cagcgccgcc
     1741 atgtcgggct gcgtgggccg catgtcgctc agggccagaa gcgcgccgtc gtcgcgcagc
     1801 agatcgccga tcgcccggcg gtcgtcctgg ctgcgtttcc agacatacca gttgtcgccg
     1861 cccaggtcct gcggcgtggt cagaagcgtg ttgtcgatat cgaacacgac cagcacgcgg
     1921 cggccccgcg cctccgcgcg caaggcctcg gcgacgggga cgcccagatc gtcggtgacg
     1981 cgctcggccg gaagggggcg atggacaggg cgcgctccgg cgacggaggt cagcagcccg
     2041 gcggcgagga caaggagaag aaggcgcttg aacatgcgcc gtttactagc gtccgcgccg
     2101 gaaaaacgca acaaatgaaa ctatctggcg cccgcattga ccaggccctg caacgccttg
     2161 agaatctcgg cgcgcaggcg cggattgtgc gggccgaggc cgccctgatg gccctgcgga
     2221 atgtgcgcga ccgggtcttc gccggtctgg aagatgtaat ggtcgaacat ggccttccag
     2281 tagcgcttgt cggcggggct gacgtccttg agcgccagca gcgcggccag gaacggcgtc
     2341 atcggattgt cggcggtcga cgcgctgttg ccccaccagt agttgaccag cacgttgaac
     2401 ggcgacagcg actggacgtg gtgccaccag tatttcggaa tatagagcgc gtcgcccggc
     2461 tccagctcgg cgacctgcgc gtgtccgagc gcctcggcca gtttcggaaa gcgctcgaaa
     2521 tcgggcgctt cgaggctggc cagcgagatg gcgcggcccg acggcgtatg atccatcggc
     2581 ccgatataga gattgccgac ctgttccggc ggaaacagcg tgaaccgccg ccgccccgcc
     2641 gcgacgcagg cgatgttgtc ggcgttgtcg ttgtgagtct gcgcgcgcgt ggcgttggag
     2701 atccagatgc gcggccatat ggccggaggc aggaaggggt gacggttctg ctcggcgaat
     2761 ccgggcagga actgcggcgt gggaacggac tggatataga tgtagggcgc gttggtctga
     2821 cccgtgacgg cgaagatgtc ttcgatgccg ccggaaacgg tcttgttgcg gcggctgaaa
     2881 ttgaactcct gcatgtcgct ggcgtaggcg aagcgccctt cggtctggcc ggaagcttcg
     2941 aggacggggg tcggctggcc tgagtcgaag cctttgaggt aatccgcgac gcgccggtcg
     3001 ccgagtcgcg cctgctgcac cacgggccag tccttcagga tggccc
//
LOCUS       DASVLL010000292         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_448121,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000292 DASVLL010000000
VERSION     DASVLL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448121"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1017
                     /gene="phoU"
                     /locus_tag="VF402_04940"
     CDS             322..1017
                     /gene="phoU"
                     /locus_tag="VF402_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX7799673.1"
                     /translation="MNPHIVKTYGEELEQLSAEVVLMGGLAEAQVADAVEAVARRDVA
                     LAQSVVGRDHRLDELEKDIERKCIRLIALRQPMADDLRKTVAAMKISTSLERIGDLAK
                     NIAKRAVVISEAEPMTPLTRSIERMGKLVSSRLRDVLDAYKSGKADLAQTVWASDTEV
                     DEHYNAMFRELLTYMMGDPRTISACTHLLFMAKNLERIGDHATNMAEHIHYELTGEDY
                     VEDRPKGDTLSPT"
     gene            1042..>1553
                     /locus_tag="VF402_04945"
     CDS             1042..>1553
                     /locus_tag="VF402_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799674.1"
                     /translation="MKPYIIIAEDEDALATLLSYNLEKEGYEVGVAVDGDEALVMVKE
                     RQPDLLVCDWMMPKVSGIEVCRRLRQVPASRNLPIVMLTARAEESDRIRGLDTGADDY
                     VVKPFSMTELMARIRAVLRRIRPGLSEDRAEFGEIVVDRMSHRVQRSGRDIHLGPTEY
                     KLLDYLMQYPR"
BASE COUNT          285 a          527 c          494 g          247 t
ORIGIN      
        1 tctggagcgc gcgcccaatg tcgccagcct gccgtccgcc gcgccgcccg ccaccttcac
       61 cgccttttcg gtctggctgc cgcagaaggg cttttacggc gtcgccgggg cggcaaccgg
      121 ccctaagccg cgtccggccg acgccgccgc gcgcgatgcg gtatgacctg tcgcattttt
      181 cgcgccgcca ttataaaact gtcacgaaat cgccgtaagg gaggccagga tcgggaaacg
      241 cgcctgcttt cgaacggccc ggctttctaa cggcaaagac gccaacccct atcttttgag
      301 gacgcaacgg tggccctgaa catgaacccg catatcgtca agacctatgg cgaagaactc
      361 gagcagcttt cggcggaggt cgtgctgatg ggcggtctgg ccgaggcgca ggtcgccgac
      421 gcggtcgagg ccgtggcgcg gcgcgacgtc gcgctggcgc aaagcgtcgt cggccgcgat
      481 caccggctgg acgagctgga gaaggacatc gagcgcaagt gcatccgcct gatcgcgctg
      541 cgccagccga tggccgacga cctgcgcaag acggtggcgg cgatgaagat ctcgacctcg
      601 ctggagcgca tcggcgatct ggccaagaat atcgccaagc gcgccgtggt gatctccgag
      661 gccgagccga tgacgccgct gacccgctcg atcgagcgca tgggcaagct ggtgtcctcg
      721 cgtctgcgcg acgtgctgga cgcctacaag tcgggcaagg cggacctggc ccagaccgtt
      781 tgggcgtcgg acaccgaggt cgacgaacat tacaacgcca tgttccgcga gctgctgacc
      841 tacatgatgg gcgatccgcg caccatctcg gcctgcacgc accttctgtt tatggccaag
      901 aacctcgagc gcatcggcga ccacgccacc aacatggccg agcatatcca ttacgagctg
      961 acgggcgagg actatgtcga ggaccgcccc aagggcgaca cgctcagccc cacctgacag
     1021 ccgccaaccg ccggaatctt catgaagccc tatatcatca tcgccgaaga cgaagacgcc
     1081 ctggcgacgc tgctgagcta caatctcgaa aaggaaggct atgaggtcgg cgtcgccgtc
     1141 gacggcgacg aagccctcgt catggtcaag gagcgccagc cggacctcct ggtctgcgac
     1201 tggatgatgc ccaaggtgtc gggcatcgag gtctgccgcc gcctgcgtca ggttccggcc
     1261 tcgcgcaatc tgcccatcgt catgctgacc gcgcgcgcgg aggagagcga ccgcatccgg
     1321 ggtctggaca ccggcgctga cgactatgtg gtcaagccgt tctcgatgac cgagcttatg
     1381 gcgcgcatcc gcgccgtgct gcgccgcata cgccccggcc tgtccgagga ccgcgccgag
     1441 ttcggcgaga tcgtcgtcga ccgcatgtcg caccgcgtgc agcggtcggg ccgcgacatc
     1501 cacctcggcc cgacggaata caagctgctc gactacctga tgcagtatcc ccg
//
LOCUS       DASVLL010000293         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_125723,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000293 DASVLL010000000
VERSION     DASVLL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125723"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VF402_04950"
     CDS             <1..686
                     /locus_tag="VF402_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HEX7799675.1"
                     /translation="GLSSAVVAHFLPQFARLDALDFGISPGQKAERGLATTRAILSYA
                     GRPLRPYAGHAKAYGWQALRRQTYPVMGRRWLADCEIPDLDLLPSAYGLSDIRFGAGL
                     ELAPLHLGLWAAAWLRRLGVPLPLEALAGPLLRISDACNAFGSDVGGMHMILRGEGVD
                     GRPLEKRWFIIAEAGDGPQIPCVPAILLARRLYEKDPGLRTGAYPCVGLTRLKDYLDA
                     LKPFAIRAF"
     gene            complement(713..1231)
                     /locus_tag="VF402_04955"
     CDS             complement(713..1231)
                     /locus_tag="VF402_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX7799676.1"
                     /translation="MSQEASSINMYQASIPIMVRALTNLKAILSKAQVWAEEKKIDEK
                     VVLNARLALDMLPFVKQVQIATDTAKGAAARLGGVDVPAYPDDEATFAELHQRLQKTI
                     DFMESVDAAGFAGSETREIVLKFPSRTIEFTGLNYLTGFAIPNLFFHVTTAYAILRHS
                     GAPLGKTDFLGG"
     gene            1409..2002
                     /locus_tag="VF402_04960"
     CDS             1409..2002
                     /locus_tag="VF402_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7799677.1"
                     /translation="MNRVLFPRFGATALALALFAAPAFTSQALAADAYTLDPTHTSVT
                     FQYTHFGYSHPTGKFMNAKGSVTLDDAHPANSSVEVSFAIDGVNTGVPALDEHLKSAD
                     FFDAAQFPTASFKSTRVEPTGPDTAKVTGDLTIHGVTRPVVLNVKLNKEAVNPMSNKK
                     AVGFTATATINRSDFGMNKYVPMVSDQVDLYIEAEAN"
     gene            2057..2857
                     /locus_tag="VF402_04965"
     CDS             2057..2857
                     /locus_tag="VF402_04965"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX7799678.1"
                     /translation="MTLPTVYLPHGGGPCFFMDWTPADMWDAMGAYLKRLPADVGATP
                     KALLIVSAHWEEAEFTVQSKPDPGLLFDYYGFPPHTYQLTWPAPGSPGLAARVQALAA
                     AADISVRADAQRDFDHGVFIPMLMAWPDAGIPTVQLSLKAGLDPAAHLALGRALAPLR
                     DEGVLIIGSGMSYHNLRRLMQRDAQGDMRHASHDFDAWLQEAVRGDRAALTRWAEAPN
                     ARLCHPREEHLLPLMVAAGAAGDDPARTTYHEDSLGPTGAAISAFQFG"
     gene            2975..3658
                     /locus_tag="VF402_04970"
     CDS             2975..3658
                     /locus_tag="VF402_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7799679.1"
                     /translation="MYGLHIANKNYSSWSLRPWVLLRTLAIPFTEHQHFFAPDNSAFR
                     AFSPSALVPCLTDGAVSVWDSLAIAEYVAEDHPRVWPEHRAARAFARSAAAEMHSGFS
                     TLRTICGMNVGVRVRLFDTPPALIRDLARIDEIWRYGLANFGGPYLAGGRFTAVDAFY
                     APVAFRIQTYGVGFDLSAEARAYVDRLLAEPAMRDWAAAAVAETQRDPGHEADLARVG
                     DIIEDSRAK"
     gene            3825..>4200
                     /locus_tag="VF402_04975"
     CDS             3825..>4200
                     /locus_tag="VF402_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799680.1"
                     /translation="MPVPAAVLADPAPQPAATPPASKAPRFAEFVALIASLMALGALG
                     IDSMLPALPVIGRDLHVQHENSLQWIVSIYFMGLGFGQLIFGILSDWLGRKRVLLVGI
                     ALYVLLGVLAGLSQNFAVLLILR"
BASE COUNT          686 a         1431 c         1338 g          745 t
ORIGIN      
        1 cgggtctgtc ctcggcggtc gtcgcgcatt tcctgccgca gttcgcgcgg ctcgatgcgc
       61 tcgatttcgg catttcgccg gggcagaagg ccgagcgcgg gctggccacc acgcgcgcca
      121 ttctgagcta tgccgggcgc cccttacgcc cctatgccgg tcatgcgaag gcctacggct
      181 ggcaggcgct caggcgccaa acctatccgg tcatgggccg gcgctggctg gccgattgcg
      241 agattcccga tctcgacctg ctgccgtccg cctacggact gtccgatatc cgcttcggcg
      301 ccgggctgga gctggcgccc ctgcatctgg gcctgtgggc ggcggcgtgg ctgaggcggc
      361 tgggcgtccc cctgccgctc gaagctttgg ccgggccgct gttgcggatc agcgacgcct
      421 gcaacgcctt cggctcggac gtcggcggca tgcacatgat cctgcgcggc gaaggcgtgg
      481 acgggcggcc gctggaaaag cgctggttca tcatcgccga agcgggcgac gggccgcaga
      541 ttccgtgcgt cccggccatc ctgctggcgc ggcggctgta tgaaaaagac cccggcctga
      601 gaaccggggc ctatccctgc gtcggcctga cgcgcctcaa ggattatctc gacgcgctga
      661 aaccgttcgc gatccgagcg ttttaaccgc ccagattaac cgcccagaaa atttaaccgc
      721 ccagaaaatc cgtcttgccc agaggcgcgc cggaatggcg caggatcgcg taggccgtgg
      781 tgacgtggaa gaacaggttg ggaatagcga agccggtcag atagttgagg ccggtgaatt
      841 cgatcgtccg cgacgggaat ttcagcacga tctcgcgcgt ctccgaaccg gcgaagcccg
      901 ccgcatcgac gctttccatg aaatcgatgg tcttttgcag gcgctgatgc agttcggcga
      961 aggtcgcctc gtcgtcggga taggcgggca cgtcgacccc gcccagacgc gccgccgcgc
     1021 ccttggccgt gtcggtcgcg atctggacct gcttgacgaa gggcagcatg tccagcgcca
     1081 gccgcgcgtt cagcacgacc ttttcgtcga tcttcttctc ctccgcccag acctgcgcct
     1141 tggacaggat ggccttgagg ttggtcagcg cgcggaccat gatcggtatc gacgcctgat
     1201 acatattgat cgacgaagct tcctgagaca tgtgttatca tcctctgatg tcaggtggaa
     1261 accgacatat gggacggcgc gcaccggttg gaagtggcgc gcgccaaaaa gatgcggcaa
     1321 aatgacgccg ccccctcttg cgggacttaa tggtaactac tattgttacg ctttggcgcc
     1381 gatacgcctc acagatggag atttcgacat gaaccgcgtc ctgttcccgc gcttcggcgc
     1441 cacggccctg gccctcgccc tgttcgccgc gcccgccttc acgtctcagg ccctcgccgc
     1501 cgacgcctat acgctggacc cgacccacac ctcggtcacc ttccagtaca cacatttcgg
     1561 ctattcgcac ccgacgggca agttcatgaa cgccaagggt tcggtgaccc ttgacgacgc
     1621 ccatccggcc aattcctcgg tcgaggtgtc gttcgccatc gacggcgtca ataccggcgt
     1681 tccggctctg gacgaacatc tgaaaagcgc ggacttcttc gacgcggcgc aattcccgac
     1741 cgccagcttc aagagcacca gggtcgagcc gaccggcccc gacaccgcca aggtcaccgg
     1801 cgacctgacg atccacggcg tcaccaggcc ggtcgtgctg aacgtcaagc tgaacaagga
     1861 agcggtcaat ccgatgagca acaagaaggc ggtcggcttc accgccaccg cgacgatcaa
     1921 ccgctccgat ttcggcatga acaagtacgt gccgatggtc agcgaccagg tcgatctcta
     1981 catcgaagcc gaagccaact gagcctgagc ggctaaaaca caagggggcg gctgcgaacc
     2041 cggccgcctt tttttcatga ccctgcccac cgtttacctg ccgcacggcg gcggaccgtg
     2101 tttcttcatg gactggacgc ccgccgacat gtgggacgcg atgggcgcgt acctgaaacg
     2161 cctgcccgcc gatgtcgggg cgaccccgaa ggcgctgctg atcgtatcgg cgcattggga
     2221 agaggccgag ttcaccgtcc agagcaagcc ggaccccggc cttctgttcg actattacgg
     2281 ctttccgccg cacacctatc aattgacgtg gccggcgccg ggcagccccg gtctggccgc
     2341 gcgcgtgcag gcgctggcgg cggcggcgga catcagcgtg cgcgccgacg cgcagcgcga
     2401 tttcgaccac ggcgtcttca tcccgatgct gatggcctgg ccggacgccg ggattccgac
     2461 ggtccagctt tcgctgaagg ccgggctgga ccccgcggcg catctggcgc tgggccgcgc
     2521 gctggcgccc ctgcgcgacg aaggcgtgct gatcatcggc tccggcatga gctatcacaa
     2581 tctgcgccgc ctgatgcaaa gggacgcgca gggcgacatg cgccacgcct cgcacgattt
     2641 cgacgcctgg ctgcaggaag cggtgcgcgg cgaccgcgcg gccctgacgc gctgggccga
     2701 ggcgccgaac gcgcgcctgt gccacccgcg cgaggagcat ctgctgccgc tgatggtggc
     2761 ggcgggcgcg gcgggcgacg atccggcgcg caccacctat cacgaggaca gcctcggccc
     2821 gaccggcgcg gcgatcagcg cgtttcagtt cggctaaagc catggcgcag tccggctgaa
     2881 cccaataggc cgtctaagcg atcggcgcag gatttccctc tggcattttt acctcgggcg
     2941 cgcgacacaa gtcccctgac agaaggagcg tccgatgtac ggcctgcata tcgccaacaa
     3001 gaactattcg tcctggtcgc tgcgtccgtg ggttctgctc aggacgctgg cgattccgtt
     3061 cacggagcat cagcactttt tcgcgccgga caattcggcc ttccgggcgt tttcgccttc
     3121 ggctctggtc ccgtgcctga cggacggcgc ggtcagcgtc tgggactcgc tggccatcgc
     3181 cgaatatgtc gccgaggacc atccgcgcgt ctggccggag catcgcgcgg cgcgcgcctt
     3241 cgcgcgctcg gcggcggcgg agatgcattc cggcttttcg accctgcgca cgatctgcgg
     3301 catgaatgtc ggcgtgcgcg tcaggctgtt cgacacgccg cccgccctga tccgcgatct
     3361 ggcgcgcatc gacgagatct ggcgttacgg cctcgccaat ttcggcggcc cctatctggc
     3421 gggcgggcgc ttcacggcgg tcgacgcctt ctatgcgccg gtcgccttcc gcatccagac
     3481 ctacggcgtc ggcttcgacc tcagcgcgga ggcgcgcgcc tatgtcgacc ggctgctggc
     3541 cgaaccggcg atgcgggact gggcggcggc ggccgtcgcc gagacgcagc gcgaccccgg
     3601 ccatgaggcc gacttggcgc gcgtcggcga catcatcgag gactcaagag cgaaatagaa
     3661 cgcttcccca aaaagcagct ccactttttg gatagggaag agcgacaaaa caaaaacttg
     3721 gagcgccgat cacgccgccg cgcccgccgg aaagaaaccg taatagctct tgcggcttcc
     3781 gcctctcgcc atcgcgggca aaaccgccta taggctgcgg cgttatgcct gttcccgccg
     3841 ccgttctcgc cgaccccgcg ccgcaacccg ccgcaacgcc gcccgcctcg aaggcgccgc
     3901 gcttcgccga attcgtcgcg ctgatcgcca gcctgatggc gctgggcgcg ctcggcatcg
     3961 attcgatgct gcccgccctg cccgtcatcg ggcgcgacct gcatgtccag catgaaaaca
     4021 gcctgcaatg gatcgtctcg atctatttca tggggctggg cttcggccag ttgatcttcg
     4081 gcatcctgtc cgactggctg gggcgcaagc gcgtcttgct ggtcgggatc gcgctttacg
     4141 tgctgctggg cgttctggcc ggactgagcc agaacttcgc cgtgctgctg atcctgcggc
//
LOCUS       DASVLL010000294         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_70984, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000294 DASVLL010000000
VERSION     DASVLL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70984"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VF402_04980"
     CDS             <1..667
                     /locus_tag="VF402_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7799681.1"
                     /translation="FLPATGTLTRLDFPPVRVESAVAAGDAVSVFYDPMIAKLIARGR
                     DRAAAIAALREALIRTRVEGVTTNLGFLRRLVSDAGFVAGDLDTGLIARRLGALLTPW
                     PDPAPCTDDFQKSDPWSDPTGWRVNLPSADPLWLETGAAGELAVTGEVRAPMPGAVLD
                     VFVAAGQAVAKGDKLLILGAMKIEHTLKAPKDGVARAVHVAAGDQVADKALLVEIDEG
                     AAP"
     gene            667..1830
                     /locus_tag="VF402_04985"
     CDS             667..1830
                     /locus_tag="VF402_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX7799682.1"
                     /translation="MDFPHLQFGHGEEIDALREAVRRFAAAEIAPLAEAADREDRFPM
                     HLWRKLGDIGALGLTAPESYGGAGLGYLAHVIVMEEISRASASIGLSYGAHANLCVNQ
                     IVRNGSAAQKAKYLPRLISGEHVGALAMSETGAGSDVMAMKLRADRRGYRYVLNGAKM
                     WITNGPDADVLVVYARTDPAAGSKGVTAFLIEKGFAGFSVAQKLDKLGMRGSHTGELV
                     FADCEAPEENVLGQAGRGAGVLMSGLDYERVVLSGGPLGIMRACLDAVLPYVHQRQQF
                     GAPVGTFQLMQGKLADMYSTWAACRAYVYAVAAACDRGETTRKDAAGCILYAAEKATW
                     MAGEAIQCLGGNGYTNDYPVGRLWRDAKLYEIGAGTSEIRRMLIGRELFEDTK"
     gene            1827..>2262
                     /locus_tag="VF402_04990"
     CDS             1827..>2262
                     /locus_tag="VF402_04990"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479522.1"
                     /note="catalyzes the formation of acetoacetate and
                     acetyl-CoA from 3-hydroxy-3-methylglutaryl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX7799683.1"
                     /translation="MSRPARVTLYEVGPRDGLQAETRIVSTADKIALIDRLSVCGLSY
                     VEAASFVSPAWVPQMADAAEVMAGIARRPGVSYPVLTPNLRGYEAARAAGAGAVAVFA
                     AASETFSRRNINCSIAESLERFRPLCAAAARDGVRVRGYASCV"
BASE COUNT          333 a          745 c          834 g          350 t
ORIGIN      
        1 ttttctgccc gcgaccggga cgctgacgcg gctcgacttt ccgcccgtgc gcgtcgagag
       61 cgcggtcgcg gcgggcgatg cggtcagcgt gttctacgat ccgatgatcg ccaagctgat
      121 cgcgcgcggt cgcgaccgcg cggcggcgat cgcggccttg cgcgaagcgc tgatccgcac
      181 gcgcgtcgaa ggggtgacga ccaatctcgg ctttctgcgc cgtctggttt ccgacgccgg
      241 tttcgttgcc ggagacctgg acacggggtt gatcgcccgg cgcctcggcg cgctgctgac
      301 gccgtggcca gatccggcgc cgtgtacgga cgattttcag aaaagcgacc cgtggtcgga
      361 cccgaccggc tggcgcgtca acctgccctc cgctgatccg ttgtggctgg agacgggcgc
      421 ggcgggcgag ctggccgtga cgggcgaggt gcgcgcgccc atgccgggcg cggtgctgga
      481 cgttttcgtc gcggcggggc aggcggtggc gaaaggcgac aagctgctga tcctcggcgc
      541 gatgaagatc gaacacacgc tgaaagcgcc gaaggacggc gtggcgcgcg ccgtccacgt
      601 cgccgccggg gatcaggtcg ccgacaaggc gctgctggtc gagatcgacg aaggagccgc
      661 gccatgatcg actttccgca tctgcaattc ggccacggcg aggagatcga cgccctgcgc
      721 gaggccgtgc gccgcttcgc cgccgccgag atcgcgccct tggccgaggc cgccgaccgc
      781 gaggaccgtt tcccgatgca tctgtggcgc aagctgggcg atatcggcgc gctgggcctg
      841 accgcgccgg aaagctatgg cggcgcgggg ctgggctatc tggcccacgt catcgtcatg
      901 gaggagattt cgcgcgcctc ggccagcatc ggcctgtcct acggcgctca cgccaacctg
      961 tgcgtcaacc agatcgtgcg caacggctcg gcggcgcaga aggcgaaata cctgcccagg
     1021 ctgatctccg gcgagcatgt cggcgcgctg gccatgtcgg agaccggcgc cggatcggac
     1081 gtgatggcga tgaagctgcg ggccgaccgg cgcggctacc gctacgtgct gaacggcgcg
     1141 aagatgtgga tcaccaacgg gccggacgcc gacgtgctgg tcgtgtacgc acggaccgat
     1201 cccgccgccg gatcgaaggg cgtcaccgcc ttcctgatcg agaagggctt tgcggggttt
     1261 tccgtggcgc agaagctcga caagctgggc atgcgcggct cgcacaccgg cgagctggtg
     1321 ttcgcggact gcgaggcgcc ggaggagaac gtgctgggtc aggccgggcg cggcgcgggc
     1381 gttctgatgt cgggcctcga ttacgagcgc gtggttttgt cgggcgggcc gctgggcatc
     1441 atgcgcgcct gcctcgacgc ggtcctgccc tacgtgcatc agcgccagca gttcggcgcg
     1501 cctgtcggaa cctttcaact gatgcagggc aagctggccg acatgtattc cacatgggcc
     1561 gcgtgccgcg cctatgtcta tgcggtcgcc gccgcctgcg accggggcga gacgacgcgc
     1621 aaggacgcgg cgggctgcat cctctacgcc gccgaaaagg cgacctggat ggcgggcgag
     1681 gcgatccagt gcctcggcgg caacggctac accaacgact atccggtcgg gcggctgtgg
     1741 cgcgacgcca agctgtacga gatcggcgcg ggcacgtcgg aaatccgccg gatgctgatc
     1801 gggcgcgaac tgttcgagga cacgaaatga gccgccccgc ccgcgtcacc ctctatgagg
     1861 tcggcccgcg cgacggcttg caggccgaga cgcgcatcgt gtcgacggcg gacaagatcg
     1921 cgctgatcga ccgattgtcc gtgtgcggac taagctacgt cgaggcggcc agcttcgtgt
     1981 cgccggcatg ggttccgcag atggccgacg ctgccgaagt catggccgga atcgcgcgca
     2041 ggccgggcgt cagctatccc gtgctgacgc cgaacctgcg gggttacgag gcggcgcgcg
     2101 cggcgggcgc cggagcggtg gccgtcttcg ccgccgcgtc cgagaccttc agccgccgga
     2161 acatcaactg ctcgatcgcg gaaagcctcg agcgtttccg cccgctctgc gcggcggcgg
     2221 cgcgcgacgg cgtgcgcgtg cgtggctacg catcgtgcgt ca
//
LOCUS       DASVLL010000295         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_71071, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000295 DASVLL010000000
VERSION     DASVLL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71071"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..2791
                     /locus_tag="VF402_04995"
     CDS             419..2791
                     /locus_tag="VF402_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7799684.1"
                     /translation="MRKLMLTGAALAALMTALMTGAAQAATVDFDSGWLFHKGDTGLG
                     PVPEAADAGWTQVNLPDDWAIKGPFSQDAATTGSGGWLPSGTVWYRKHFTLPADASGK
                     DVAIRFDGVMERSGVWINGHHLGFRPSGYATFQYDLTPYLKAGDNVISVRADTSSQPA
                     SRWYQGGGVYRHVHLIVQDKVHLVTDGTAISTPSIAADSAAVAIVSEAVNTSGAPVTV
                     HLDITLTGPDGKPVASFQSDAQTLAPGATAKLSAQGSLANPKLWNLDAPNLYTARVAL
                     VAESGLRDESDLNFGIRNMQFRADTGFWLNGKNIKLKGVAIHADGGAFGMAVPLDVYE
                     RRLSILKSLGVNAIRTAHHPFSPEFLDLCDRMGFVVMDEAFDMWTVAKNPQDYHLFFS
                     DWSSEDARTMARRDRNHPSIALWSIGNEIHDTPDADSAKATLGRLRDVFHAEDPTRPV
                     TQALFRPNVSHDYDDGLADMLDVVGQNYRENELAKAHADKPSRRIIGTENTKDRGPWV
                     TVRDNPAYSGMFLWTGIDYLGEADRAGWPYISNPSGLIDRDGVVKAIGLERASWWSQK
                     PFIAIARRVARTKDIAGAVGAEVNQAASRALPGAFADWSPAESGAHTEKVEVYSNAPT
                     AELFLNGKSLGKKPINADASARVWDVSFAPGTLKAVGYDEKGRKIAENTLQTAGAPAA
                     VKLVAEESRLAPGFDHIGFVRAEIVDAKGVLVPDAQTPITVSVGGAGALAAFDNGSPI
                     DHTLFDAPTRDAFNGQALIMVRATGTNGAISVTASAPGLTSGAASLQAEE"
     gene            2794..>3322
                     /locus_tag="VF402_05000"
     CDS             2794..>3322
                     /locus_tag="VF402_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEX7799685.1"
                     /translation="MRALCGLAAVLLVAPAALAQDRTDPALPPVAAHITVDAGHAVGA
                     LPPIWRFFGADEPNYATMKDGRKLLDHLGALRPGDVYFRAHNLLNTGDGTPAFKWGST
                     NIYTEDKTGKPVYDFAVTDRILDAYIARGVHPYLQLGFMPEALSSAPKGEPYQHGWRP
                     GFAYNLIDGAWNAPPK"
BASE COUNT          583 a         1219 c         1030 g          490 t
ORIGIN      
        1 gccgtctccg cgttcagccc gtgttcgccc gacagcgaga acaacaaacc gtccggcccc
       61 tcggcccgcg ccgctccggc cagcgcgcac agggcggtcg cagtcagcaa aaggcggcgc
      121 ggaaatccca tctcgatcaa ccctgtctgt cgcccctccg ccgagggcta ttctctttat
      181 aaacgccctc tccccgccat gaggggacga ggacgaggag ccgcccggcg actgagcgca
      241 accggccgcc gcaagtctta ccgcaggccc gataaactgc aagaggcgtt tcgcattttt
      301 gatcatatgc gcgcatttca atgcaatttg gatcatgagc gatcttgccg ccgcgggcgc
      361 gccttgttaa cctgcggccc ggccggggag cccaaaaacg atcagggata ggaaaacaat
      421 gcgcaaactc atgctgaccg gcgcggcgct ggccgcactc atgaccgccc tcatgactgg
      481 ggccgctcag gcggcaaccg tcgatttcga ctcaggctgg ctgttccaca agggcgacac
      541 cggcctcggc cccgtccccg aagccgccga cgccggctgg acgcaggtga acctgcccga
      601 cgactgggcg atcaaggggc cgttcagcca ggacgcggcg accaccggct ccggcggctg
      661 gctgcccagc ggcacggtct ggtaccgcaa gcatttcacc ctgcccgccg acgcttccgg
      721 caaggacgtc gccatccgct tcgacggcgt gatggagcgt tcgggcgtgt ggatcaacgg
      781 ccatcatctc ggcttccgcc cgtccggcta tgcgacgttc caatacgacc tgacgccgta
      841 cctgaaggcg ggcgacaatg tgatctcggt gcgcgccgac acctcgtcgc aaccggcctc
      901 gcgctggtat cagggcggcg gcgtttaccg ccacgtccac ctgatcgtgc aggacaaggt
      961 ccacctcgtc acggatggaa ccgccatctc caccccgtcc atcgccgccg acagcgccgc
     1021 cgtcgccatc gtcagcgagg cggtcaacac gtccggcgcg ccggtgaccg tgcacctcga
     1081 catcaccctg accggcccgg acggcaagcc ggtcgccagc ttccagagcg atgcgcagac
     1141 cctcgccccc ggcgcgaccg cgaaactcag cgcgcaaggc tcgcttgcga atccgaagct
     1201 atggaatctc gacgcgccga acctctatac cgccagggtc gccctggtcg cggaatccgg
     1261 cttgcgcgac gaaagcgacc tgaatttcgg catacggaac atgcagttcc gcgccgacac
     1321 cggcttctgg ctgaacggga aaaacatcaa gctcaagggc gtggcgatcc acgccgacgg
     1381 cggcgccttc ggcatggccg tgccgctcga cgtctatgaa cgccgcctgt cgatcctgaa
     1441 aagcctcggc gtcaacgcga tccgcaccgc ccaccatccg ttctcgccgg agttcctcga
     1501 cctctgcgac cgcatgggct ttgtggtgat ggacgaggcg ttcgacatgt ggaccgtggc
     1561 caagaatccg caggactatc acctgttctt ctccgactgg tcgagcgagg acgcgcggac
     1621 catggcgcgc cgcgaccgca accacccctc catcgccctc tggtcgatcg gcaacgagat
     1681 ccacgacacc cccgacgccg acagcgccaa ggcgacgctt ggccgcctgc gcgacgtctt
     1741 ccacgccgaa gacccgacgc gcccggtgac tcaggccctg ttccgcccga acgtcagcca
     1801 cgattacgac gacggcctgg ccgacatgct ggacgtggtg ggccagaact accgcgagaa
     1861 cgaactggcc aaggcccacg ccgacaagcc gtcgcgcagg atcatcggca ccgagaacac
     1921 caaggatcgc ggcccgtggg tcacggtgcg cgacaatccc gcctattcgg gcatgttcct
     1981 gtggaccggc atcgactatc tgggcgaggc cgaccgcgcg ggctggccct acatctccaa
     2041 cccgtccggc ctgatcgacc gcgacggcgt ggtcaaggcc atcgggctgg agcgcgcctc
     2101 gtggtggtcg caaaagccct tcatcgccat cgcccggcgc gtggcgcgca cgaaggacat
     2161 cgccggcgcg gtcggcgccg aggtcaatca ggccgccagc cgcgccctgc ccggcgcctt
     2221 cgccgactgg tcgcccgccg aaagcggcgc gcataccgaa aaggtcgagg tctattccaa
     2281 cgcgccgacg gccgagttgt tcctcaacgg caaatcgctg ggcaaaaagc cgatcaacgc
     2341 cgacgcctcg gcgcgcgtct gggacgtcag cttcgcgccc ggaacgctga aagcggtcgg
     2401 ctatgacgaa aagggccgca agatcgccga aaacacgctg caaaccgccg gcgccccggc
     2461 ggcggtgaag ctggtcgcgg aggaaagcag gctcgccccc ggcttcgacc atatcggctt
     2521 cgtccgcgcc gagatcgtcg acgcgaaggg cgttctggtc cccgacgcgc aaaccccgat
     2581 caccgtgtcg gtcggcggcg cgggcgcgct tgccgccttc gacaacggca gcccgatcga
     2641 tcacaccctg ttcgacgcgc cgacgcgcga cgctttcaac ggtcaggctt tgatcatggt
     2701 gcgcgcgacc ggcacgaacg gcgcgatcag cgtcacggcg tccgcgcccg gcctgacctc
     2761 cggcgcggcc agcctgcagg cggaggaata agcgtgcgcg cgctctgcgg cctcgccgcc
     2821 gtccttctgg tcgcgcccgc ggctttggcg caggaccgca ccgacccggc cctgccgccc
     2881 gtcgcggccc atatcacggt ggacgcgggc catgccgtcg gcgccctgcc gccgatctgg
     2941 cgcttcttcg gcgcggacga gccgaactac gccaccatga aggacgggcg caagctgctc
     3001 gatcatctgg gggccttgcg ccccggcgac gtctatttcc gcgcgcacaa cctgctgaac
     3061 accggcgacg gaaccccggc gttcaagtgg ggctccacca atatctacac cgaggacaag
     3121 accggcaagc cggtctatga cttcgccgtc accgaccgca tcctcgacgc ctatatcgcg
     3181 cgcggcgtgc atccctacct gcaactgggc tttatgcctg aggccctgtc gtccgcgccg
     3241 aagggcgagc cgtatcagca tggctggcgc cccggcttcg cctacaacct gatcgacggc
     3301 gcctggaacg cgccgccgaa gg
//
LOCUS       DASVLL010000296         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_393291,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000296 DASVLL010000000
VERSION     DASVLL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393291"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF402_05005"
     CDS             <1..508
                     /locus_tag="VF402_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799686.1"
                     /translation="GAFVVANVRSARAWSLMANLDLPLDGFMPGGLLRLHYMRQDSRV
                     TDPVTGAVRRLNATQPVNASASFRQDLRIARLSWGVDYARGWRGFSWYADEARLDRHA
                     PELGLFMETGRFLNAKLRVEVQGLTGRRDDFHRQLYAPNRAGLPARNELWEIDTPTAL
                     SVSLTRNF"
     gene            complement(514..1677)
                     /locus_tag="VF402_05010"
     CDS             complement(514..1677)
                     /locus_tag="VF402_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7799687.1"
                     /translation="MISFKVLLLTGALAAAPVLSQAATASPDPCPSAPKAAIALAEKV
                     ADYQLATMAGEQAVRDGRNADPTGWVQGALFVGLAALADHSERPEFKQAVMARGAANG
                     WRLGARKYDADDYVIGQSYIWAATHGAGDAAVAQVRVRLDAVLADPPKTDLTFVDDGK
                     TCRDRWCWADALFMGPAVWFGMSRLTHDPKYADYAKSEFEATTAKLYDPDEHLYYRDS
                     RFFTRRDDRGQKMFWSRGDGWVFAGLANMIRLLPEGDAERAKMVVIFREMAVKLKAIQ
                     KPDGYWSPSLLGDPKTSLPESSGTGFYTYGFAWGIKAGILDAASYEPVVRKGWGALTR
                     AVHPDGKLGYVQPVSDRPDAVTYDDTQLYGVGAFLLAATAVADLDLPPDPAAR"
BASE COUNT          282 a          609 c          609 g          298 t
ORIGIN      
        1 cggcgccttc gtcgtcgcca atgtgcgttc cgcccgcgcc tggagcctga tggcgaatct
       61 ggacctgccg ctcgacgggt tcatgcccgg cggtctgctg aggctgcatt atatgcggca
      121 ggactcccgc gtcacggacc cggtcacggg ggcggtccgg cggctcaacg ccacgcagcc
      181 tgtcaatgcc agcgcgtcgt tcaggcagga tctgcgcatc gcgcggctgt cctggggcgt
      241 ggattatgcg cgcggctggc gcggcttctc ctggtacgcg gacgaagcgc gtctggaccg
      301 tcacgcgccg gaacttggcc tgttcatgga aacgggccgt tttctgaacg ccaagcttcg
      361 cgtggaggtt cagggactga ccggccggcg cgacgacttc caccgccagc tctatgcgcc
      421 gaaccgcgcg ggtcttccgg cccggaacga actctgggag atcgacacgc ccacggccct
      481 atcggtttcg ctcacccgga acttttgacg ccgtcaccgc gccgccgggt cgggaggaag
      541 gtccagatcg gcgaccgccg tggcggccag cagaaaggcg ccgacgccgt aaagctgggt
      601 gtcgtcgtag gtgaccgcgt cgggacggtc gctgaccggc tggacgtagc ccagcttgcc
      661 gtcaggatgc accgcgcgcg tcagggcgcc ccagcccttg cgcaccaccg gttcgtagct
      721 cgccgcgtcg agaatccccg ccttgatccc ccaggcgaag ccgtaggtgt aaaagcccgt
      781 gccgctcgat tccggcagcg aggtcttcgg atcgccgagc agcgagggcg accagtagcc
      841 gtccggtttc tggatcgcct tcagcttgac ggccatctcg cggaagataa cgaccatttt
      901 cgcgcgttcg gcgtcgcctt cgggcaaaag ccggatcata ttggccagcc cggcgaacac
      961 ccagccgtcg ccgcggctcc agaacatttt ttgccccctg tcgtcgcggc gcgtgaagaa
     1021 acggctgtcg cggtaataga ggtgttcgtc cgggtcgtag agcttggccg tggtcgcctc
     1081 gaactcggac ttggcgtagt cggcatattt cggatcgtgg gtcaggcggc tcatgccgaa
     1141 ccacaccgca gggcccatga acagggcgtc ggcccagcac cagcggtcgc ggcacgtctt
     1201 gccgtcgtcg acgaaggtca ggtcggtctt cggcggatcg gccagcacgg cgtcgaggcg
     1261 cacgcggacc tgagccacgg ccgcgtcgcc cgcgccgtgc gtcgccgccc agatgtagct
     1321 ctggccgatg acgtaatcgt cggcgtcgta cttccgcgcg cccagccgcc agccgttcgc
     1381 cgcgccgcgc gccatgacgg cctgtttgaa ttcgggacgc tcgctatggt cggccagcgc
     1441 cgccagcccg acgaacaggg cgccctgcac ccagccggtg ggatcggcgt tccggccgtc
     1501 ccggacggcc tgttcgcccg ccatggtcgc cagttgatag tcggcgacct tttccgcgag
     1561 ggcgatggcg gccttcggcg cggaggggca tggatccggc gacgccgtcg cggcctgaga
     1621 gagaaccggc gccgcagcca gagcgcctgt cagaagaagc accttaaacg agatcatggc
     1681 caacctttcc tcaggtcacg gactcaggcc gcacgcgcgt caggcgcggc gacaggatgt
     1741 gcaggatcgc gaaccctagc agataagcgc cgcccgcgat aacgaagatg ggctgata
//
LOCUS       DASVLL010000297         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_179753,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000297 DASVLL010000000
VERSION     DASVLL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179753"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VF402_05015"
     CDS             <1..426
                     /locus_tag="VF402_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7799688.1"
                     /translation="LAGAKEKGIKAVFDYRAAADSVFVDRVQIQQVLINLIRNAGEAM
                     RAAPRRELTIATSMPSETTIAVDVADTGPGIAEDIQQQLFQPFVTSKPGGMGIGLTIS
                     KRIIEAHDGEISVSRNAEGGATFRFTLPVSDGSDEDEPA"
     gene            423..1031
                     /gene="fixJ"
                     /locus_tag="VF402_05020"
     CDS             423..1031
                     /gene="fixJ"
                     /locus_tag="VF402_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HEX7799689.1"
                     /translation="MMERVIHIVDDEAPVRSSLAFLLMSAGFAVRVHESGPAFLDALQ
                     DPGAICLVTDLRMPDMTGVELLEALQDRGVSLPAIVVTGHGDVPMAVAAMKAGATDFI
                     EKPFSDAVLIDAIRRACEKAGNPSDGAAPVAVVQQRLGQLTAREQDILKGIVAGLPNK
                     TIAYDLEISPRTVEVHRASIMHKMQARNLPDLVRMTLILERK"
     gene            complement(1050..>1672)
                     /locus_tag="VF402_05025"
     CDS             complement(1050..>1672)
                     /locus_tag="VF402_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7799690.1"
                     /translation="HQRLIDASALPGLMEKTGQFVFIAERLSDMGYRAVGIDHFAKPD
                     DGLSRALADRTLRRNFMGYTDQPNDRLIALGASSISQLDEGIAQNVNRGTSYERLVRG
                     GGLAATRGWAYTGDDRARSAVITSLMCFFEVDLGKIVQTYALPPDHFAADLAAMSAFV
                     EAGLVSVAGQVVRFHAPYKMLVRSVASVFDRYARTGAEGNRYSRVA"
BASE COUNT          315 a          549 c          535 g          273 t
ORIGIN      
        1 ctcgccggcg ccaaggaaaa ggggatcaag gccgtcttcg actatcgggc ggcggcggac
       61 agcgtgttcg tcgaccgggt ccagatccag caggtgttga tcaatctcat tcgcaacgcc
      121 ggcgaagcca tgcgcgccgc cccccggcgc gaactgacca tcgccacgtc catgccctcg
      181 gaaacgacga tcgcggtcga tgtcgcggat acgggccccg gcatcgccga ggatatccag
      241 cagcaactct ttcagccttt cgtcaccagc aaacccggtg gcatgggcat cgggcttacg
      301 atatcaaagc ggatcataga ggctcacgac ggcgaaatca gcgtcagccg caacgccgaa
      361 ggcggcgcga ctttccggtt tacgctcccg gtctcggatg gatcagacga ggacgagccc
      421 gcatgatgga acgcgtcatc catatcgtcg acgacgaagc gccggtcagg tcttccctgg
      481 ccttcctgct gatgagcgcg ggcttcgccg tgcgcgttca cgaaagcggc ccggcctttc
      541 tggacgcgct tcaggatccg ggcgccatat gccttgtcac ggatctgcgc atgccggaca
      601 tgaccggcgt cgagctgctg gaggcgctgc aagaccgcgg cgtttccctg cccgccattg
      661 tcgtcaccgg gcatggcgac gtgcccatgg cggtggcggc catgaaggcg ggcgcgaccg
      721 atttcatcga gaagccattt tccgacgcgg tcctgatcga cgccatccgc cgggcatgcg
      781 aaaaggccgg aaatccgtcc gacggcgcgg cgcccgtcgc cgtcgttcag cagcgcctcg
      841 gccagctcac ggccagggag caggacatcc tgaagggaat cgtcgccggg ctgcccaaca
      901 agaccatcgc ctacgacctg gagatcagtc cgcgcaccgt cgaggtgcat cgcgccagca
      961 tcatgcacaa gatgcaggcc aggaacctgc ccgatctcgt tcggatgacc ctgatcctcg
     1021 agcgcaaata gcctgtcgaa gtccgcccat caggcgacgc gcgaatagcg gttgccttcc
     1081 gcgccggtcc gcgcatagcg atcgaacacg ctggcgaccg accgaacgag catcttgtaa
     1141 ggcgcgtgaa agcgcacgac ctgaccggcg accgagacca gaccggcctc gacaaatgcg
     1201 ctcatggccg ccagatcagc ggcgaagtga tccgggggca aggcataggt ctggactatt
     1261 ttgccaagat ccacctcgaa gaagcacatc aggctggtga tgacggccga tcgggcgcgg
     1321 tcgtcgccgg tataggccca gccgcgcgtg gcggcgaggc cgccgccgcg gaccagccgt
     1381 tcgtaactcg tcccccggtt gacgttctgg gcgatcccct cgtccaactg gctgatggac
     1441 gaggcgccca gcgcgatcaa gcggtcgttc ggctgatcgg tgtatcccat gaagttgcgg
     1501 cgcagcgtcc tgtcggcaag ggcgcgggac aggccgtcat cgggtttcgc gaaatggtcg
     1561 atcccgacgg cgcgatagcc catgtccgac aggcgttcgg cgataaagac gaactggccc
     1621 gtcttttcca tgagtcccgg cagcgcggac gcgtcgatca accgttgatg ct
//
LOCUS       DASVLL010000298         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_393856,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000298 DASVLL010000000
VERSION     DASVLL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393856"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VF402_05030"
     CDS             complement(<1..395)
                     /locus_tag="VF402_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX7799691.1"
                     /translation="MPLETQPADPTEKPGILPMQGLNALIAAGAIRSATPFDADQVQP
                     ASLDLRLGARAWRVRASFLPRGRTVAERLKDVEMHEIDLTRGAVFESGCVYIAELQES
                     LSLPAGISARANPKSSTGRVDVFVRLLSD"
     gene            570..968
                     /gene="apaG"
                     /locus_tag="VF402_05035"
     CDS             570..968
                     /gene="apaG"
                     /locus_tag="VF402_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX7799692.1"
                     /translation="MPYQAVTHDIAVSVEVAYIPPEQHGRGPGRHVWTYHIRIENRGA
                     RTVQLKTRHWTIVDARGHVEHVDGPGVVGETPVLAPGGSFAYTSGCPLPTDSGAMSGH
                     YMFERDDGVWLKVAIPAFSLDAPSAKRTLN"
     gene            complement(1179..1562)
                     /locus_tag="VF402_05040"
     CDS             complement(1179..1562)
                     /locus_tag="VF402_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX7799693.1"
                     /translation="MRSLTEYYDELVCSHCRSKRVRVESRSGKALLDPDTVVECMRCG
                     LPIPLARLDAQPGVNTCVACAEDAVRPYTEAWPQPPNAFRKCPRCKSPTEVRQNTVDQ
                     SFFLGCKTWPRCPWTMPYETARKPI"
BASE COUNT          283 a          516 c          511 g          278 t
ORIGIN      
        1 tggtcgctga gcaggcgcac gaacacgtcg acgcggcccg tggacgattt cggattggcg
       61 cgcgccgaaa ttccggcggg caggctcagg ctttcctgca actccgcgat atagacgcag
      121 cccgattcga acaccgcgcc gcgcgtcagg tcgatttcgt gcatctcgac atccttgaga
      181 cgctcggcca cggtgcgccc gcgcggcagg aaggaggcgc gcacccgcca cgcccgcgcg
      241 ccgaggcgca ggtcgaggct ggccggctgc acctgatcgg cgtcgaaagg cgtcgcgctg
      301 cggatcgcgc ccgccgcgat cagggcgttc agcccctgca tcggcaggat gccgggcttt
      361 tcggtcggat cggcgggctg ggtttcgagc ggcatggcga tgtctttgcg ggaaaggcga
      421 aatctgtcgg gactggccat ttaagagagg tttgtcgcca cagcaagctt tatgcgcgcg
      481 ggctcgtccc acacgggacc ggcgcccttg ccaaacgcgg cgcatcgccg catttgagat
      541 atcgagacgc cacaacagcg aatgcgccga tgccctatca agccgtcacc cacgacatcg
      601 ccgtttcggt cgaggtcgcc tacatcccgc cggaacagca cggccgcggg ccgggccgcc
      661 atgtctggac ctaccatatc cgcatcgaga accgcggcgc gcgcacggtg cagctcaaga
      721 cgcgccactg gaccattgtc gacgcgcgcg gccatgtcga gcatgtcgac ggccccggcg
      781 tggtcggcga aacgccggtt ctggcgccgg gcggcagctt cgcctacacc tccggctgcc
      841 cgctgccgac ggactcgggc gcgatgagcg gccactacat gttcgaacgc gacgacggcg
      901 tctggctgaa ggtcgccatc cccgccttct cgctggacgc gccaagcgcg aaacgcacgc
      961 tgaactaggg tcggtcacga aaagcgcgaa ggacaggcga ttgacatgcg gcttggcgga
     1021 cggctcttta ttgaagccga aagcgccctc gaccggcgcg tcttctcagg cgttcaactc
     1081 ttgagccttg ttgaagctga tgttccagcg acggtttgct tgttcagacg acacgatatc
     1141 aggcgcccaa acacccacga aagctggcta ttccgtgatc agatcggctt gcgcgccgtc
     1201 tcgtaaggca ttgtccaggg acaacgcggc caggtcttgc aaccgaggaa aaaagattgg
     1261 tcgacagtgt tttgccgcac ctcggtcggg cttttgcatc gagggcattt gcgaaacgca
     1321 tttggcggct gcggccacgc ttccgtatag gggcgcacag cgtcctcggc gcaggcgacg
     1381 caggtattga cgccgggctg cgcgtccagg cgcgccagcg gtatcggcag tccgcaacgc
     1441 atgcattcga caactgtatc gggatccagg agcgcttttc ccgaacggct ttcgacacgc
     1501 acgcgcttgc tgcggcaatg gctgcacacc agctcgtcat aatattcggt caggctccgc
     1561 atgtcgaaat cttcgcctcg gagttcgc
//
LOCUS       DASVLL010000299         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_503193,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000299 DASVLL010000000
VERSION     DASVLL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503193"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VF402_05045"
     CDS             complement(<1..651)
                     /locus_tag="VF402_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX7799694.1"
                     /translation="MPRVAYVNGAYVRNADAGVSINDRGYLFADAVYEVWSVFDGRLA
                     DSEGHLNRLDRSLRELQIVQPMTRAALLNVLNEVIRRNRITEGMVYLQISRGVAPRDH
                     VFPSHTPPGVVITARPVDREAAARKAEAGVRAISAPDIRWGRCDIKTVGLLPNVLAKQ
                     AARERGAGEVIFVDKDGFVTEGGSTNIFMVARDGVIRTRPLNANILPGVTRLNLLSI"
     gene            complement(653..2053)
                     /locus_tag="VF402_05050"
     CDS             complement(653..2053)
                     /locus_tag="VF402_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7799695.1"
                     /translation="MKVSSGVDILVVDDEADIRELIAGILEDEGYAVRTAADSNAALR
                     AIKARKPSLCVLDIWMQGGGLDGLELLSVIHELDPDIPAIMISGHGTIETAVSAIKKG
                     AFDFIEKPFKTERLIMVVQRALEITNLRRENKRLRSQAVVPDGLVGNSAAAQLLRSTI
                     AKVAPANSRVMISGPAGSGKELVARMIHEASPRARCEFVPISAAGMTPERLDVELFGE
                     EGEGGRPRKIGVFERAHGGTLFLDEVADMPPESQSRILRVLVEQRFTRVGGAQDVQVD
                     VRVISSTSKDLPAEISAGRFREDLFHRLNVVPVRVPGLSERRGDIAELVTYFIDRISA
                     AQGLPKRILSEDAMATLQVHEWPGNVRQLRNNIERLLILASGNPSDPITAEMLPSEVV
                     ESAVAGAIRPERIIALPLRDAREVFEREYLSSQIVRFGGNISRTASFIGMERSALHRK
                     LKSLGLAHIRGDEDEA"
     gene            complement(2053..4323)
                     /locus_tag="VF402_05055"
     CDS             complement(2053..4323)
                     /locus_tag="VF402_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7799696.1"
                     /translation="MKDSRRRWRLWLSRNLRSIRAAGPQRLLLGAGYGAAILTTAFVM
                     WLIVAAPGTGPISQVGDYLLILICLNLVLLLGLLSIVVMRVVRLARARGADAGARLHL
                     RFVSLFSLAAVAPAIIVALSFGLLNRGIDTWFSDRVKTTVENGAAIAQSYLEAQHDTS
                     SHALTHLQQLVESPDARKLFPDRLAFSIALKNAFIERGDLDAVYIVDSSGQVLARAEG
                     DNAPPYMAPPAEAIADAKAKGAVGFTAPDAERILAPLRDYNGAILYGLKTWPPGILEK
                     LKSSSQAVTDYKGTIKSKGRAQTIFSLAYIEAVLVVLIGAIWGGASAAESIAVPVARL
                     VQAAGKVAAGDLNARVATSHQSEDIAVLSRAFNHMTSDLQSQQAALKSAGEEAESRRR
                     FIETVLSEISAGIVGLDVLEHVSAINRHAANFLNVSAEDALGRNIRDIAPEIAELLNK
                     VSVHRVEEQEVDLVRQGETRRVRVRASALETGGAVLTFDDITRLIAAQRNAAWKDVAR
                     RIAHEIKNPLTPIQLSAERLRRKYRGQIETDGDTFDRLTDTIVRQVGDIGRMVDEFSS
                     FARMPAPNFAEEEADELIRACVFSQRVARPDISVEIVEPLPEVKVVCDGRMLSQALGN
                     VLKNGGEAISSRLLRDGATETGGDQVVGHLRVEMRLDGNEMIIEVEDDGVGLPEKDRD
                     RLTEPYVTTREKGTGLGLAIVKRILEDHGGEFYLTDAVVLNGARAVLRLPRQQAGQPP
                     HAAEGSDSGPQKMMRV"
BASE COUNT          737 a         1543 c         1499 g          763 t
ORIGIN      
        1 aatcgacaac aggttcagcc gcgtcacgcc gggcaggatg ttggcgttga gcggccgcgt
       61 cctgatcacg ccgtcgcgcg cgaccatgaa gatattggtc gagccgccct cggtgacgaa
      121 gccgtccttg tcgacgaaga tgacctcgcc cgcgccgcgt tcgcgcgccg cctgcttggc
      181 cagcacgttc ggcagcaggc ccaccgtctt gatgtcgcag cggccccagc ggatgtccgg
      241 cgcgctgatg gcgcggacgc ccgcctcggc cttgcgcgcg gcggcctcgc ggtccaccgg
      301 acgcgccgtg atcaccacgc cgggcggggt atgcgacgga aagacgtgat cgcgcggcgc
      361 cacgccccgg ctgatttgca aatagaccat gccttctgtg attctattgc ggcggatgac
      421 ctcgttcagc acgttcagca gcgccgcccg cgtcatgggt tgcacgatct gcaactccct
      481 gaggcttctg tcgagccggt tgagatggcc ctcgctgtcg gccagccgtc cgtcgaacac
      541 cgaccagacc tcatagaccg cgtcggcgaa caggtagccg cggtcgttga tcgacacccc
      601 ggcgtcggcg ttgcgcacat aggcgccgtt gacataggcg acgcgcggca tgttacgcct
      661 cgtcctcgtc gccgcggata tgggccaggc ccagcgactt gagcttgcgg tgcagggcgg
      721 aacgctccat gccgatgaag ctggctgtgc gcgagatgtt gccgccgaag cgcacgatct
      781 gcgacgacag atattcgcgc tcgaacacct cgcgcgcgtc gcgcagcggc agggcgatga
      841 tgcgttcggg ccggatggcg cccgccaccg ccgattccac cacctcggac ggcagcatct
      901 cggcggtgat cgggtctgac ggattgcccg acgccaggat cagcaggcgt tcgatattgt
      961 tgcgtaactg gcgcacattg cccggccatt catgcacctg aagcgtcgcc atcgcgtcct
     1021 cggacaggat gcgtttgggc aggccctgcg ccgcgctgat gcggtcgatg aaataggtga
     1081 ccagttcggc gatgtcgccg cgacgttccg acaggcccgg cacgcgcacc ggcacgacat
     1141 tgagccggtg gaacagatcc tcgcggaaac gcccggcgga aatctcggcg ggcaggtctt
     1201 tcgaggtcga ggaaatgacg cgcacgtcga cctgcacgtc ctgcgcgccg ccgacgcggg
     1261 tgaagcgctg ctcgaccagc acgcgcagga tgcggctctg gctttccggc ggcatgtcgg
     1321 ccacctcgtc gaggaacagg gtgccgccgt gcgcgcgctc gaacacgccg atcttgcgcg
     1381 ggcgcccgcc ctcgccctcc tcgccgaaca gttcgacgtc gaggcgctca ggcgtcatgc
     1441 ccgccgccga gatcggcacg aattcgcagc gggcgcgcgg actggcctcg tggatcatgc
     1501 gcgcgaccag ctccttgccg gaacccgccg gaccggagat catgacgcgc gaattggccg
     1561 gcgccacctt ggcgatggtc gagcgcagaa gctgcgccgc cgccgaattg ccgaccagcc
     1621 cgtccggcac gaccgcctgc gagcgcagcc gcttgttctc gcggcgcaga ttggtgatct
     1681 ccagcgcgcg ctgcaccacc atgatcagcc gttcggtctt gaacggcttt tcgatgaagt
     1741 cgaaggcgcc cttcttgatg gccgaaaccg ccgtttcgat ggtgccgtgg ccggagatca
     1801 tgatcgccgg aatatcggga tcgagttcgt ggatcacgct gagcagctcc agcccgtcga
     1861 ggccgccgcc ctgcatccag atgtccagca cgcataagga cggcttgcgc gccttgatcg
     1921 cgcgcagggc cgcgttcgaa tcggcggcgg tgcgcaccgc atagccttcg tcctcgagga
     1981 tgcccgcgat cagttcgcga atatcggcct cgtcgtccac gaccagaata tcaacgccag
     2041 acgatacttt catcagaccc tcatcatttt ttgcggcccg ctgtcggagc cttccgccgc
     2101 atggggaggc tgtcccgcct gctggcgcgg cagacgcagg acagcccgcg cgccgttcaa
     2161 aacaaccgcg tcggtgagat aaaactcgcc gccgtggtct tccagaatac gcttcacaat
     2221 cgccaggccg aggcccgtgc ccttttcccg cgtcgtcaca tagggttcgg tcagccggtc
     2281 gcgatccttt tccggcaggc cgacgccgtc gtcctcgacc tctatgatca tttcgttgcc
     2341 gtccagccgc atctcgacgc gcaaatggcc gacgacctga tcgccgccgg tttcggtcgc
     2401 gccgtcgcgc aggagccgcg acgagatcgc ctcgccgccg tttttcagca cattgcccag
     2461 cgcctgcgac aacatgcgcc cgtcgcagac caccttcact tcgggcagcg gctcgacgat
     2521 ctcgaccgaa atgtcgggcc gggccacgcg ctgcgaaaac acgcaggcgc ggatcagttc
     2581 gtcggcctcc tcctcggcga agttcggcgc gggcatgcgc gcgaaggacg agaattcgtc
     2641 gaccatgcgg ccgatatcgc cgacctgacg cacgatggtg tcggtcaggc ggtcgaaggt
     2701 gtcgccgtcc gtctcgatct ggccgcgata tttgcggcgc aggcgttcgg cggacaactg
     2761 gatgggggtc agcggattct tgatctcgtg cgcgatgcgc cgcgccacgt ccttccacgc
     2821 ggcgttgcgc tgcgcggcga tcaggcgcgt gatgtcgtcg aaggtcagca ccgccccgcc
     2881 ggtttccagc gccgaggcgc gcacccgcac gcgccgcgtc tcgccctgcc gcaccagatc
     2941 gacctcctgc tcctcgacgc gatggacgct gaccttgttg agcagttcgg cgatttcggg
     3001 cgcgatgtcg cggatgttgc gccccagcgc gtcctcggcg ctgacgttga ggaagttggc
     3061 ggcgtggcgg ttgatggccg agacatgctc cagcacgtcg aggccgacga tgcccgcgct
     3121 gatttccgac agcaccgtct cgatgaagcg gcggcggctc tcggcctcct cgcccgccga
     3181 tttcagcgcc gcctgctggc tttgcaggtc ggaggtcatg tggttgaagg cgcgcgacag
     3241 gacggcgatg tcctcggact ggtgcgaggt ggcgacgcgc gcgttgaggt cgccggcggc
     3301 caccttgccc gccgcctgca ccagccgcgc gaccggcacg gcgatgctct ccgccgccga
     3361 ggcgccgccc cagatggcgc cgatcagcac caccagcacg gcctcgatat aggccagcga
     3421 aaagatggtc tgggcgcggc ccttgctctt gatcgtgccc ttgtagtcgg tgaccgcctg
     3481 gctggaggat ttgagctttt ccagaatacc gggcggccag gtcttcaggc catagagaat
     3541 ggcgccgtta tagtcgcgca gcggcgccag aatacgctcg gcgtccggcg cggtgaagcc
     3601 gaccgcgccc ttggccttgg cgtcggcgat ggcctcggcg ggcggcgcca tatagggcgg
     3661 cgcgttgtcg ccctcggcgc gcgccagcac ctgacccgac gaatcgacga tatagaccgc
     3721 atcgaggtcg ccgcgctcga taaaggcgtt tttcagcgcg atggaaaagg ccagccggtc
     3781 cgggaacagc ttgcgcgcgt ccgggctttc gacaagctgc tgcaaatgcg tcagcgcatg
     3841 ggacgaggtg tcgtgctgcg cctccagata ggactgggcg atggcggcgc cgttctccac
     3901 ggtcgtcttg acgcggtcgg agaaccaggt gtcgatgccg cgattcaaaa gcccgaagga
     3961 cagcgccacg atgatcgccg gcgccacggc ggccagcgag aacagcgaca cgaaacgcag
     4021 gtgcagccgc gcgcccgcat cggcgccgcg cgcccgcgcc agccgcacga cgcgcatgac
     4081 cacgatcgac agaaggccca gcagcagcac gaggttcagg cagatcagga tcagcagata
     4141 gtcgccgacc tgactgatcg gtcctgtccc cggcgcggcg acgatcagcc acatgacgaa
     4201 ggccgtggtc aggatggccg cgccgtagcc tgcgccgagc agcagtcttt gcggccccgc
     4261 cgcgcgaatg ctgcggaggt tgcgcgacag ccacaggcgc cagcgtcgtc ttgagtcctt
     4321 cacgaaacgt cccgcttgat gaaacgatgc gtggcatttg cgcactactg tgtctgaaaa
     4381 tcaacagcaa tgttgccgat aagcggcaaa tcgccgtcct gcttcagcgc atcagcgcgc
     4441 gcgcctcggc ctcgatccgc gccggatcgt cgaggcggca caggcgaccc ttgtcctgac
     4501 gccgcgcctg cggatcggga tggtgcggcg aggcttcgat at
//
LOCUS       DASVLL010000300         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_287743,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000300 DASVLL010000000
VERSION     DASVLL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287743"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="VF402_05060"
     CDS             <1..105
                     /locus_tag="VF402_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX7799697.1"
                     /translation="AKLKQWRRIATRYDKLAANFLGFVKLASIMLWIK"
     gene            complement(273..491)
                     /locus_tag="VF402_05065"
     CDS             complement(273..491)
                     /locus_tag="VF402_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799698.1"
                     /translation="MRALFVTTGLFAAALLMTGCADLERASRPADAYTMPDKPLSLPK
                     CAAPQAGVKAQGPNSPTPMQQEAATNCR"
     gene            complement(546..1250)
                     /gene="dnaQ"
                     /locus_tag="VF402_05070"
     CDS             complement(546..1250)
                     /gene="dnaQ"
                     /locus_tag="VF402_05070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX7799699.1"
                     /translation="MAIEIVLDTETTGIDPRKGHRLIEIGCIEIEDLLPTGRTFHHYI
                     DPEREIEPDAIRVHGIDNDKVRGKPKFAAVMEALLDFIGDKRLIAHNAPFDRGFINME
                     LERHGRAPTPDDQWLDTLDMARKRFPGAANSLDALCRRYNISLAERDLHGALIDARLL
                     ASVYLELLGGKERGLNLEMAAAVAAAAEAAAHTGYGPRPRPLKSRIHPEEAQAHAQFV
                     AKYLKDKALWPQADPA"
     gene            complement(1252..1842)
                     /gene="coaE"
                     /locus_tag="VF402_05075"
     CDS             complement(1252..1842)
                     /gene="coaE"
                     /locus_tag="VF402_05075"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080696.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7799700.1"
                     /translation="MIRLGLTGSIGMGKSTLAAMFADEGVPVWDADAAVHRLYAEDDA
                     LKASLREAFGDVLTDNRVDRIKLSAALHKDPNGFERLNALVHPRVAGDRAAFFARHAD
                     APVVLADIPLLYETAAERHLDKVVVASAPHATQRTRVLARPGMTEARFAAILERQIPD
                     AEKRRRADYVVDTSQTLDACREQVRDMIAELKGKGA"
     gene            complement(1839..>2142)
                     /locus_tag="VF402_05080"
     CDS             complement(1839..>2142)
                     /locus_tag="VF402_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7799701.1"
                     /translation="FDLTQVSRAFADAGAVINAASGGPHPPLDALPDAAAVMDMTYRP
                     LKTAFLQSAEARGLATVDGLNMLIEQARPSFQTFYGVEPWPGFDIRALALTFLGEA"
BASE COUNT          364 a          656 c          738 g          384 t
ORIGIN      
        1 gccaaactaa aacagtggcg acgcatcgcc acacgatatg acaaactcgc cgccaatttt
       61 cttggcttcg tcaaactcgc cagtatcatg ctatggatta aataattaaa ttgtcaccac
      121 agcctagcgt ttccccaaaa agtggctcca ctttttggat aaggaagagc gacaaaacaa
      181 aacttagagc gccgatctga tgggatcaga tcggcgctct aaaagccctg acgataaaaa
      241 agcccggcgc cgcaaggtcc gggctttttt ctttaccggc agttggtggc cgcttcctgc
      301 tgcatcggcg tggggctgtt cgggccttgc gcctttacgc ctgcctgcgg cgccgcgcat
      361 ttgggcaggc tcaacggctt gtcgggcata gtgtaggcgt cggccgggcg gctggcgcgc
      421 tccagatcgg cgcaaccggt cataagcagg gcggcggcga aaaggccggt ggtgacgaaa
      481 agcgcgcgca tgatccaaaa cccccaattg tatgatgaaa gaagatcacc gcattatggc
      541 gaagatcagg ccggatcggc ctgcggccac agcgccttgt ccttgaggta tttggcgacg
      601 aattgcgcgt gcgcctgcgc ttcctccggg tggatgcggc ttttgagcgg gcgcgggcgc
      661 gggccgtaac cggtatgggc ggcggcttcg gcggcggcgg ccacggcggc ggccatttcg
      721 aggttgaggc cgcgctcctt gccgcccaaa agttcgagat agaccgaggc cagcaggcgg
      781 gcgtcgatca gcgcgccgtg caggtcgcgt tcggccagcg agatattgta gcggcggcac
      841 agggcgtcga gcgaattggc cgcgccggga aagcgtttgc gcgccatgtc cagcgtgtcg
      901 agccactgat cgtccggcgt cggcgcgcgc ccgtgccgct caagctccat attgatgaag
      961 ccgcggtcga acggcgcgtt gtgcgcgatc aggcgcttgt cgccgataaa gtcgagcagg
     1021 gcctccatca ccgcggcgaa tttcggcttg ccgcgcacct tgtcgttatc gatgccgtgg
     1081 acgcggatgg cgtccggctc gatctcgcgc tcggggtcga tatagtggtg gaaggtgcgc
     1141 ccggtcggca gcagatcctc gatctcgatg cagccgattt cgatcaggcg gtggcccttg
     1201 cgcggatcga tgccggtggt ttcggtgtcg agaacgattt cgatggccat gtcaggcgcc
     1261 tttccccttc agctccgcga tcatgtcacg cacctgttcg cggcaggcgt caagggtctg
     1321 cgacgtatcg acgacgtagt cggcgcggcg gcgcttttcc gcgtcgggga tctgccgttc
     1381 caggatggcg gcgaagcggg cctcggtcat gccgggacgc gccagcacgc gcgtgcgctg
     1441 cgtggcgtgc ggcgcgctgg ccacgaccac cttgtcgaga tggcgctccg ccgccgtctc
     1501 gtacagcagc ggaatgtcgg ccagcacgac cggcgcgtcg gcgtggcgcg cgaagaaggc
     1561 ggcgcgatcc ccggccacgc gcggatgcac cagcgcgttc aggcgctcga agccgttggg
     1621 gtctttgtgc agggcggcgg acagcttgat ccggtcgacg cgattgtccg tcagcacgtc
     1681 gccgaaggct tcgcgcaacg acgccttcag cgcgtcgtcc tcggcgtaaa gccggtgcac
     1741 ggcggcgtcg gcgtcccaga cgggcacgcc ttcatcggcg aacatggcgg cgagggtcga
     1801 tttgcccatg ccgatggagc cggtcaggcc cagcctgatc atgcttcacc gagaaaggtc
     1861 agcgccagcg cgcggatatc gaagcccggc cacggttcga cgccgtaaaa cgtctgaaac
     1921 gacgggcgcg cctgttcgat cagcatgttc aggccgtcga ccgtggcgag gccgcgcgct
     1981 tcggcgcttt gcaggaaggc ggttttgagc gggcgatagg tcatgtccat gaccgccgcc
     2041 gcgtccggca gggcgtcgag cggcggatgc ggcccgcccg aagcggcgtt gatcaccgcg
     2101 cccgcgtcgg cgaaggcgcg cgaaacctgc gtcaggtcga ag
//
LOCUS       DASVLL010000301         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_18577, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000301 DASVLL010000000
VERSION     DASVLL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18577"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF402_05085"
     CDS             <1..186
                     /locus_tag="VF402_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7799702.1"
                     /translation="GHDRRYAIDATRLESELGWRARETFDTGLERTVRWYLDNESWWR
                     PLRDNAYAGERLGLVAP"
     gene            183..1082
                     /gene="rfbD"
                     /locus_tag="VF402_05090"
     CDS             183..1082
                     /gene="rfbD"
                     /locus_tag="VF402_05090"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX7799703.1"
                     /translation="MSLRLAVTGRHGQVARSLIARAAQEDVVIVPLARPDFDPSRPKS
                     FMDAVRAARPDAIVSAAAYTAVDRAESEPELAEAVNGRAPGELGRVAAALNVPILHLS
                     TDYVFSGDKPEPYVETDATGPINVYGHSKLHGERALAAASDNHVILRTSWVFSPEGRN
                     FVNAMLALAQTRDALDVVADQRGCPTSADEIARALIAVARRVIADPAPELRGVFHMAG
                     GAETNWAAFAEAVFDGARRRGWPAAAVRPTPSSAWPSPARRPMNSRLDSTRLRSNFGV
                     SLDPWPVSLDKHLNRLVELRQRT"
     gene            1079..1954
                     /gene="rfbA"
                     /locus_tag="VF402_05095"
     CDS             1079..1954
                     /gene="rfbA"
                     /locus_tag="VF402_05095"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX7799704.1"
                     /translation="MKGIILAGGHGSRLFPMTLGVSKQLMPIYDKPMIYYPLSTLMLA
                     GIREILIISTPHDLPLFRGFLGDGAQWGLSLAYAEQPHPHGLAEAYRIGADFVDGQPS
                     CLVLGDNIFYGSSLPSYLEDAATIETGARVFAYQVSDPQRYGVVEFDERMRAVSIQEK
                     PAQPRSNWAVTGLYFYDHQVVDIAVNLVPSARGELEITDINNAYLQRGRLTVQPIGRG
                     YAWLDTGTPDSLLDAAEFVRVIEKRQGFKIACPEEIAWRKGYIDDTQMRAAIRRLEHS
                     DYGRYLARLFQAGHD"
BASE COUNT          341 a          651 c          671 g          366 t
ORIGIN      
        1 ggccacgacc gccgctacgc catcgatgcg acccggctgg agagcgaact gggctggcgc
       61 gcacgcgaaa ccttcgacac cgggctggag aggacggtgc gctggtatct cgacaacgaa
      121 tcctggtggc ggccgttgcg cgacaacgcc tatgcgggcg agcgtctcgg actggtggcg
      181 ccgtgagcct gcgtctggcg gtgacgggac gccacgggca ggtggcgcgc agcctcatcg
      241 cgcgcgcggc tcaggaagat gtcgtcatcg ttccgctggc gcgtccggat ttcgatccgt
      301 cgcgtccgaa aagctttatg gacgctgtgc gcgcggcgcg acccgacgcg atcgtctcgg
      361 cggcggccta cacggcggtg gaccgggcgg agagcgagcc cgaactggcg gaagccgtca
      421 atggacgcgc gccgggcgaa ctgggcaggg tcgccgccgc gctgaacgtt cctatcctgc
      481 atctatccac cgattacgtg ttttcgggcg acaagcctga gccctatgtc gagacggacg
      541 cgaccggacc gatcaacgtc tatggccaca gcaagctgca tggcgaacgc gcgctggcgg
      601 cggcgagcga taaccatgtc atcctgcgca catcgtgggt tttctcgccc gaggggcgca
      661 atttcgtcaa cgccatgctg gctctggcgc aaacgcgcga tgcgctcgac gtggtcgccg
      721 atcagcgcgg ctgcccgact tcggcggatg agatcgcgcg cgcgctgatc gccgtcgcgc
      781 gtcgcgtcat cgccgatccg gcgcccgaac tgcgcggcgt gttccatatg gcgggcggcg
      841 cggaaacgaa ctgggcggcg ttcgccgaag ccgttttcga cggcgcgcgc cgccgtggct
      901 ggcccgccgc cgcggtcagg cccacgccgt cctccgcatg gccgtcccca gcgcgccgac
      961 cgatgaattc gcggctcgac agcacgcgcc tgcgatcaaa tttcggcgtc agtcttgatc
     1021 cctggccggt ttcgctcgat aaacacctga atcgtcttgt ggaattaagg cagcgtacat
     1081 gaaaggcatc attctggcgg gcggacacgg cagtcggctt ttcccgatga ccctgggcgt
     1141 gtccaagcag cttatgccga tctacgacaa gccgatgatc tattatccgc tgtcgaccct
     1201 gatgctggcg ggcattcgcg aaatcctgat catctccacg ccgcacgatc tgccgttgtt
     1261 tcgcgggttt ctgggcgacg gcgcgcaatg gggcctgtcg ctcgcctatg ccgagcagcc
     1321 gcatccgcac ggcctggcgg aagcctatcg catcggcgcc gatttcgtcg acggccagcc
     1381 gtcctgcctc gtactgggcg acaatatttt ttacggatcg tccctgccgt cctacttgga
     1441 agacgcggcg acgatcgaga cgggcgcgcg agttttcgcc tatcaggtct ccgaccccca
     1501 gcgttacggc gtggtcgagt tcgacgagcg gatgcgcgcc gtgtccattc aggagaagcc
     1561 cgcgcagccg cgctcgaact gggcggtgac cggtctttac ttctatgatc atcaggtggt
     1621 ggacatcgcc gtgaatcttg tgccgtcagc gcgcggcgag ttggagatca ccgacatcaa
     1681 caacgcctat ctgcaacgcg ggcggttgac ggtgcagccg atcggacgcg gctatgcgtg
     1741 gcttgacacc ggaacgcccg acagcctgct cgacgccgcc gaattcgtgc gcgtcatcga
     1801 aaagcgccag ggcttcaaga tcgcctgtcc ggaggaaatc gcctggcgca agggctatat
     1861 cgacgatacg cagatgcgcg ccgcgataag gcgattggag catagcgatt acggccgcta
     1921 tctcgcccgg ctttttcagg ccggacacga ttaagctttt atagtcgcca catccccgaa
     1981 cgcgacgaag tgggggttgg cgtaggcgcc gtcgccggag tcgaagcgt
//
LOCUS       DASVLL010000302         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_18658, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000302 DASVLL010000000
VERSION     DASVLL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18658"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VF402_05100"
     CDS             complement(<1..238)
                     /locus_tag="VF402_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7799705.1"
                     /translation="MTLVSIPANPVPEDVVSGTIKTPDGAELRFARWAPPAGRKGTVC
                     VFTGRGETIEKYFETVRDLRDRGFAVAMFDWRGQG"
     gene            474..950
                     /locus_tag="VF402_05105"
     CDS             474..950
                     /locus_tag="VF402_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX7799706.1"
                     /translation="MRTYDLTPFYRSTVGFDRLFSLLDQVTSDGSPGYPPYNIERTGE
                     NEYRITVAVSGFSQAELSIVAKENTLTIKGEKSANENGKDKAEVLYRGIAARAFERIF
                     QLADFVQVKNASLEHGLLHVDLVREIPEAKKPRNIPIGSGASAPQVVDGSAEKAAA"
     gene            complement(1192..>1694)
                     /locus_tag="VF402_05110"
     CDS             complement(1192..>1694)
                     /locus_tag="VF402_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799707.1"
                     /translation="QHGFFYSIAVTDRRGDNGRLVIDARNGRIARFVPAYRWGPDFRS
                     GPGLAYGPSWRMPPMVGPAPRPPAPVPVPKLASRTPAAVPLPRPDPVHPGEVKSQVKP
                     EAKPLAAKPMPPERQQSAVNQVKPAEAAPAPTRPAAAPVEAKPATPQQPDILPTQEMP
                     KVQGFE"
BASE COUNT          298 a          559 c          531 g          306 t
ORIGIN      
        1 ggccctggcc gcgccaatcg aacatcgcga ccgcgaaacc gcgatcgcgc aaatcgcgca
       61 cggtctcgaa atatttttcg atcgtctcgc cgcgcccggt gaagacgcag acggtcccct
      121 tgcgccccgc aggcggcgcc cagcgcgcaa agcgcagttc cgcaccatcg ggcgtcttga
      181 tcgtgccgct gacgacgtct tccggaacgg gattggcggg aatcgagaca agggtcatgg
      241 gtgggagccg ataggcaaaa ggcgccgaaa atcaagggcg tgtgcaacaa aaacggcgcg
      301 ttccggcccc tcttgaacgc ccccctggct cacccatatc accttcgtgc aggccgtcac
      361 cagcgtttcg gggtccggta ttggaactgg gacgttcaag gctgcacagg actaaagccc
      421 ggcccgatgg cgggcgggtt attccaacag tcgctcaatg gaggactatt cctatgcgta
      481 cctatgacct cacccccttc tatcgttcca ccgtcggctt cgaccgtctt ttctccctgc
      541 tcgatcaggt gacctcggac ggcagccccg gctatccgcc ttataatatc gagcgcaccg
      601 gcgagaacga gtatcgcatt acggtcgccg tatccggctt ctcacaggcc gagctttcga
      661 tcgtcgcgaa ggaaaatacg ctgacgatca agggcgagaa atccgccaac gagaacggca
      721 aggataaagc cgaggtgctt taccgcggca tcgcggcccg tgccttcgag cgcatcttcc
      781 agctcgccga tttcgtgcag gtgaagaacg cctcgctcga gcacggcctg ctccatgtcg
      841 atctcgtccg cgagatcccc gaggccaaga agccgcgcaa cattccgatc ggcagcggtg
      901 cctccgctcc gcaggtcgtc gatggctcgg cggagaaggc tgcggcctaa ccaccaaatt
      961 tgctcggcag gtattggctg agacgacaac gccccgggaa accggggcgt ttttgtttgg
     1021 gcgtctctct agctttgatt ctggttacgt gtccgtcatt ccggggggcg acgcggagcg
     1081 tcgaacccgg aatcccgaag ttgcggcacg agattccccg atgggcaact gcgcctctga
     1141 gctctggtgc taacgcacca tcccggaatg accacttcac ttcggataac ctcactcgaa
     1201 gccctgcacc ttcggcattt cctgcgtcgg caggatgtcc ggctgctgcg gcgtcgcggg
     1261 cttcgcctcg accggcgcgg ccgccgggcg ggtcggcgca ggcgccgctt ctgccggttt
     1321 gacctggttg acggcggatt gttgccgctc cggcggcatg ggcttcgccg cgagcggctt
     1381 cgcctcgggc ttcacttgag acttcacttc gcctggatga accgggtccg gcctcggcag
     1441 cggtactgcg gcaggcgtgc ggctcgccag tttcggcacc ggcacgggcg ccggcggacg
     1501 cggcgcgggt cccaccatcg gtggcattcg ccaggatggg ccataggcca ggccaggacc
     1561 ggagcgaaaa tctggccccc agcgataggc cggcacgaag cgggcgatcc gcccattgcg
     1621 ggcgtcgatc accagccggc cattgtcgcc gcgccggtcg gtcaccgcga tcgagtagaa
     1681 aaagccgtgc tgcc
//
LOCUS       DASVLL010000303         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_449394,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000303 DASVLL010000000
VERSION     DASVLL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449394"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VF402_05115"
     CDS             <1..743
                     /locus_tag="VF402_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX7799708.1"
                     /translation="DTTNDPIIASRAPQNWRTEQRSEDYYNEGLLIWLDADTLIREKT
                     GGRRSLDDFAKAFFGVRDGDWGVDTYTFDDVVKALNDVYPYDWATFLHTRLDGHGEAQ
                     GGGAPLDGLTRGGYKLAYTETPSDYFNALQAYHKYENFNYSIGFIVSNKDGGLSDVMW
                     DGPAFKAGLVPGVTVVAVDGKAYSAQRLKAAITAAKTSKEPIQLLVKSGDDYKTVGVD
                     YHGGLRYPVLQPINGAPKRLDDILAAKK"
     gene            781..1539
                     /locus_tag="VF402_05120"
     CDS             781..1539
                     /locus_tag="VF402_05120"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX7799709.1"
                     /translation="MIRLFIAEPTPVFAAGLRLTLNPDQGRYLTTVMRRVAGDEVGLF
                     NGRDGEWRARIVEAGKKAVTLELLERLRAQPAPGDLTLAVALIKRAALEYIVEKATEL
                     GVARIQLLITRRSNADHTRVDRLTAIAREAAEQTERLDVPEILPPVRLDAWLARDGAE
                     TIVFGDEESTHEDSMHEGGARATPPLLQVASGLSGPVAVLIGPEGGFDDDERKTLRSM
                     AKTRAVNLGPRILRADTAAIAALTLCQAAAGDWR"
     gene            1654..>2827
                     /locus_tag="VF402_05125"
     CDS             1654..>2827
                     /locus_tag="VF402_05125"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX7799710.1"
                     /translation="MAVTTEAKPVRTMDDLVSYFASGEKPREDWRIGAEHEKFAFRLS
                     DLRRPEYAGPDGIQAMLKGLMRFGWKPYEEQGKLIALTRNGASVTLEPGGQFELSGAP
                     LETIHDICAETGQHLEEVKMVASELGLGFIGLGFSPLWTRAETPVMPKGRYNIMRDYM
                     PKKGGLGLDMMLRTCTVQANLDYESEADMVKKFRVSLALQPLATALFANSPFTEGKPN
                     GFLSFRANVWTDTDPDRTGMLDFVFDEDFGYEAYARYALSAPMYFVKRDVPENGGYID
                     ASGQDFKAFLKGDLPALPGELPTMDDWADHLTTLFPEVRLKKYLEMRGADGGPWSRLC
                     ALPALWGGLLYDSAALDQAWQLVKGWSKADHNTLRGMAAKTGLKGDIAGRPVRDWAL"
BASE COUNT          517 a          865 c          988 g          457 t
ORIGIN      
        1 tcgacaccac caacgatccg atcatcgcct cgcgcgcgcc gcagaactgg cgcacggagc
       61 agcgttcgga ggactattat aacgaggggc tgctgatctg gctcgacgcc gatacgctga
      121 tccgcgaaaa gaccgggggg cgaaggtcgc tggacgattt cgccaaggcc tttttcggcg
      181 tgcgcgacgg cgactggggc gtcgatacct atacattcga cgatgtggtc aaggcgctga
      241 acgacgtcta tccctacgac tgggcgacct tcctgcacac gcggctggac ggccacggcg
      301 aggcgcaggg cggcggcgcg ccgctggacg ggctgacgcg cggcggctac aaactggcct
      361 atacggagac gccgtcggac tatttcaacg cgcttcaggc ctatcataag tacgagaatt
      421 tcaactattc gatcggcttc atcgtcagca acaaggacgg cggcctgagc gacgtgatgt
      481 gggacggacc ggctttcaag gccgggctgg ttccgggcgt gacggtggtg gcggtcgacg
      541 gcaaggcgta cagcgcgcaa aggctgaaag cggcgatcac ggcggcgaaa acgtcgaagg
      601 agccgatcca attgctggtg aagtcgggcg acgactataa gacggtcggc gtcgattatc
      661 acggcggcct gcgctatccg gtcttgcagc ccatcaacgg cgcgcccaaa cggctggacg
      721 acatactggc ggcgaagaag taggcttgag gcgaacggag gatcggctta caccgtgggc
      781 atgatccgcc tgttcatcgc cgaacccacg ccggttttcg ccgccggcct gcgcctgacg
      841 ctcaatcccg atcagggccg ttacctcacg acggtgatgc gccgcgttgc gggcgacgag
      901 gtcggcctgt tcaacgggcg cgacggcgaa tggcgcgcgc gcatcgtcga agccggaaag
      961 aaggccgtga cgctggagct gctcgagcgc ctgcgcgcgc agcccgcgcc gggcgatctg
     1021 acgctggccg tggcgctgat caagcgcgcg gcgctggaat acatcgtcga gaaggccacc
     1081 gaactgggcg tggccaggat tcaactgttg atcacccggc gcagcaacgc cgaccatacg
     1141 cgcgtcgacc ggctgaccgc catcgcgcgc gaggccgccg aacagaccga gcggctggac
     1201 gtccctgaga tcctgccgcc ggtgaggctg gacgcgtggc tggcgcggga cggggcggag
     1261 acgatcgttt tcggcgacga ggagtcgacg catgaggatt caatgcatga gggtggcgcg
     1321 cgcgcgacgc cgccgctgtt gcaggtcgcg tcgggcctga gcgggccggt ggccgtgctg
     1381 atcgggccgg agggcggctt cgacgacgac gagcgcaaga ccttgcgcag catggcgaag
     1441 acgcgcgcgg tcaatctggg gccgcgcatc ctgcgcgccg acacggcggc catcgcggcg
     1501 ctcaccctat gtcaggcggc ggcgggcgat tggcggtaat atcgggcggt aatataaaaa
     1561 ccggcgtcgg cttgccagag cgcgaggcgc cgcccatata ggcgttgcct taattggggg
     1621 catcaactgg atagtcggaa agagcagggg cgcatggcgg tgacaactga ggcgaagccg
     1681 gtccgcacga tggacgatct cgtcagctat ttcgccagcg gggaaaagcc gcgcgaagac
     1741 tggcgcatcg gggccgagca cgagaagttc gccttccgcc tcagcgatct gcgccgcccc
     1801 gaatacgccg gtcccgacgg catccaggcc atgctgaaag ggctgatgcg cttcggctgg
     1861 aagccctatg aggagcaggg caagctgatc gccctgacgc gcaacggcgc ctcggtgacg
     1921 ctggagccgg gcgggcagtt cgagctgtcc ggcgcgccgc tggagacgat ccacgatatc
     1981 tgtgcggaga cgggccagca cctcgaagag gtcaagatgg tggcctcgga gcttggcctc
     2041 ggctttatcg gcctcggctt ctcgcccctg tggacgcggg cggaaacgcc ggtcatgccc
     2101 aagggccgct acaacatcat gcgcgactac atgccgaaaa agggcgggct gggcctcgac
     2161 atgatgctgc gcacctgcac ggtgcaggcc aatctcgact acgaatccga agccgacatg
     2221 gtgaagaagt tccgcgtctc gctggccttg cagccgctgg ccaccgcctt gttcgccaat
     2281 tcgccgttca ccgagggcaa gccgaacggc tttttgtcct tccgcgccaa tgtgtggacc
     2341 gacaccgacc cggaccgcac cggcatgctc gatttcgtgt tcgatgaaga cttcggttat
     2401 gaggcctatg cgcgttacgc gctgagcgcg ccgatgtatt tcgtcaagcg cgacgtgccg
     2461 gaaaacggcg gttatatcga tgcttccggg caggacttca aggcgtttct caagggcgat
     2521 ctgcccgccc tgccgggcga attgccgacg atggacgact gggccgatca tctgacgact
     2581 ttgttcccgg aagtgcgtct caagaaatat ctcgaaatgc gcggcgccga cggcgggccg
     2641 tggagccgcc tgtgcgcctt gcccgcgctg tggggcggct tgctgtacga ttccgccgcg
     2701 cttgatcagg cgtggcaact ggtgaaaggc tggtcgaagg ccgatcacaa caccctgcgc
     2761 ggcatggcgg cgaagaccgg cctcaagggc gatatcgccg ggcggccggt gcgcgactgg
     2821 gcgctgg
//
LOCUS       DASVLL010000304         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_394243,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000304 DASVLL010000000
VERSION     DASVLL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394243"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VF402_05130"
     CDS             complement(<1..586)
                     /locus_tag="VF402_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7799711.1"
                     /translation="MTVIPGQTSLKQNPKDRRMSEDRPDAPATVDDGAAFEPAQDVAF
                     RSPISQGRLHGALAHKLGVDILKGAYKPGETLPNEIESSSALEISRSAYREAIRILAA
                     KGMVESRPKAGTRVTERARWNILDPEVLRWMFETEPSHEFITGLFELRLITEPAAAAL
                     AAQRREPKHLDIMDKALNVMASQTLATEAGRKADL"
     gene            696..1862
                     /locus_tag="VF402_05135"
     CDS             696..1862
                     /locus_tag="VF402_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7799712.1"
                     /translation="MKIAVSGLGYVGLSNAVLLAQHNEVVALDVAPEKVDLLNARKAP
                     IADAELEDYLAHKDLNLKATLDKREAYAGADFVIIATPTNYDPDSNYFDTRSVQAVIR
                     DVLEISPSSVTVIKSTIPVGFVEEMRERFACDNLIFSPEFLREGRALYDNLHPSRIVV
                     GERSERAEAFAGLLRQGAVKRDIPVLFTGASEAEAIKLFANTYLAMRVAYFNELDTYA
                     ALAGLNTREIIDGVGLDPRIGAHYNNPSFGYGGYCLPKDTKQLLANYSEVPQNLIRAI
                     VDANTTRKDFIAADIVRRNPKVVGVYRLTMKANSDNFRDSSIQGVMKRIKAKGIEVVV
                     YEPALKDEQFFRSRVIRDLNQFKAMCDVILSNRRSPELADVADKIYTRDLFGVD"
     gene            complement(1859..3454)
                     /gene="cimA"
                     /locus_tag="VF402_05140"
     CDS             complement(1859..3454)
                     /gene="cimA"
                     /locus_tag="VF402_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX7799713.1"
                     /translation="MSERVYLYDSTLRDGAQAQGIDFSVADKQAIASALDAFGIDYIE
                     GGWPGANPTDDAFFAALPKTKTARISAFGMTRRAGRSASNDPGLQDLLAVQTPSICLV
                     GKTWDWQVETALKVSTAENLRMIADSLAHVRSNGREAVFDAEHFFDGYKANPAYAIEA
                     LKAAQEGGAQWLVLCDTNGGSMPSEVGRIVAAVKDALPDANLGIHCHNDTEQAVANSL
                     AAVEAGARQVQGTINGIGERCGNANLIAIIPTLMLKMGFDTGLSAEQLTHIGELSRLV
                     DDRLNRAPDRHAAYVGASAFAHKGGLHVSAMNKDSRSYEHIDPALVGNTRLILVSDKA
                     GRANIVNRLHQMGIAVADDDDHIGELVKAVKERESLGYAYEDASASFELMARERLGLV
                     PRYYDLQSYRALNEYRLDAKGRAFDVAEATVKLSVAGEQAMTVAEGNGPVNALDAALR
                     KALLPHYPQLAKMHLNDYKVRILTPSDATAALTRVMIESRDDQGRTWNTLGVSHNVID
                     ASYAALCDAITFHLLKSGAAPKV"
BASE COUNT          577 a         1167 c         1195 g          665 t
ORIGIN      
        1 cgaggtcggc cttgcgcccg gcctcggtgg ccagggtctg cgacgccatg acgttcagcg
       61 ccttgtccat gatgtcgaga tgcttcggct cgcgccgctg cgccgccagc gcggcggcgg
      121 ccggttcggt gatcaggcgc agctcgaaca ggccggtgat gaattcgtgg ctcggctcgg
      181 tctcgaacat ccagcgcagc acttcggggt cgagaatgtt ccagcgcgcg cgctcggtca
      241 cgcgcgtgcc cgccttgggg cggctttcga ccatgccctt ggcggccagg atgcggatcg
      301 cctcgcgata ggccgagcgc gagatttcca gcgcgctgct cgattcgatc tcgttgggca
      361 gcgtctcgcc gggtttgtag gcgcctttga ggatgtccac gcccagcttg tgcgccagcg
      421 cgccgtgcag gcgcccctgc gagatcgggc tgcggaaggc gacgtcttgc gccggctcga
      481 acgcggcgcc gtcgtccacg gtcgcggggg catcggggcg gtcttcggac atgcggcggt
      541 ctttcgggtt ctgcttcagc gaggtctgtc cgggtatgac agtcatgtta cgacttgaca
      601 agacggaacg gagggcggac gcgccttgaa aaaggcggcg aattgcgctg taaccgcagc
      661 ccctgccgcg tgtcgcggcg ccggagacga acgtgatgaa gatcgccgta tcgggtcttg
      721 gctatgtcgg cctgtccaac gccgtgctgc tggcgcagca taatgaggtt gtcgcgctgg
      781 acgtcgcgcc cgaaaaggtc gatctgctca acgcgcgcaa ggcgccgatc gccgacgccg
      841 agcttgagga ctatctggcg cataaggatc tgaacctgaa agcgacgctg gacaagcgcg
      901 aagcctatgc gggcgccgat ttcgtcatta tcgccacccc gaccaattac gacccggact
      961 ccaactattt cgacacgcgc tccgtgcagg cggtgatccg cgacgtgctg gagatttcgc
     1021 cgtcctcggt caccgtgatc aagtcgacca tcccggtcgg cttcgtcgag gagatgcgcg
     1081 aacgcttcgc ctgcgacaac ctgatcttct cgccggaatt cctgcgcgag ggccgcgcgc
     1141 tctacgacaa cctgcatccg tcgcgcatcg tggtcggcga gcgctccgaa cgcgccgagg
     1201 cgttcgcggg ccttttgcgg cagggcgcgg tgaagcgcga catcccggtg ctgttcaccg
     1261 gcgccagcga ggccgaggcg atcaagctgt tcgccaacac ctatctggcc atgcgcgtgg
     1321 cctatttcaa cgaactggac acctatgcgg cgctggccgg gctgaacacg cgcgagatca
     1381 tcgacggggt cggcctcgat ccgcgcatcg gcgcgcatta caacaacccg tccttcggct
     1441 atggcggcta ttgcctgccc aaggacacca agcagttgct ggccaactat tccgaggtgc
     1501 cgcaaaacct gatccgcgcc atcgtcgacg ccaacaccac gcgcaaggac ttcatcgccg
     1561 ccgacatcgt gcgccgcaat cccaaggtcg tcggcgtcta tcgcctgacc atgaaggcca
     1621 attccgacaa tttccgcgac tcctcgattc agggcgtgat gaagcgcatc aaggccaagg
     1681 gcatcgaggt cgtggtgtat gagccggcgc tcaaggacga gcagttcttc cgttcgcgcg
     1741 tgatccgcga ccttaaccag ttcaaggcga tgtgcgacgt catcctgtcc aaccggcgct
     1801 cgcccgaact ggccgacgtc gccgacaaga tctatacccg cgacctgttc ggcgtggatt
     1861 agaccttcgg cgccgcgccg gacttgagca ggtggaaggt gatggcgtcg cacagcgccg
     1921 cataggaggc gtcgatgaca ttgtggctga cgcccagcgt gttccacgtc cggccctgat
     1981 cgtcgcggct ttcgatcatc acgcgcgtca gggcggcggt ggcgtccgac ggggtgagga
     2041 tgcgcacctt gtaatcgttc aggtgcatct tcgccaattg cggatagtgc ggcaacagcg
     2101 ccttgcgcag cgcggcgtcc agcgcgttga ccgggccgtt gccctcggcc accgtcatcg
     2161 cctgctcgcc cgcgacgctc agcttcacgg tcgcttcggc cacgtcgaag gcgcggcctt
     2221 tcgcgtccag ccggtattcg ttgagcgcgc gatagctttg cagatcgtaa tagcgcggga
     2281 ccaggccgag gcgttcgcgc gccatcagct cgaagctggc gctggcgtcc tcataggcgt
     2341 agccgagcga ctcgcgctcc ttcaccgcct tgaccagttc gccgatatgg tcgtcatcgt
     2401 cggccacggc aatgcccatc tggtgcaggc ggttgacgat attggcgcgt cccgccttgt
     2461 ccgagaccag gatcaggcgc gtattgccga ccagggccgg gtcgatatgc tcgtaggagc
     2521 ggctgtcctt gttcatcgcg ctgacgtgca gcccgccctt gtgcgcgaag gccgaggcgc
     2581 cgacataggc ggcgtggcgg tcgggcgcgc gattgagccg gtcgtcgacc agccgcgaca
     2641 attcgccgat atgggtcagt tgctccgccg acagcccggt gtcgaagccc attttcagca
     2701 tcagggtcgg gatgatggcg atcagattgg cgttgccgca gcgttcgccg atgccgttga
     2761 tcgtgccctg cacctggcgc gcgcccgcct cgaccgcggc cagcgaattg gccaccgcct
     2821 gctcggtgtc gttatgacag tggatgccga ggttggcgtc gggcagggcg tccttgaccg
     2881 ccgcgacgat gcgtcccacc tcggacggca tggagccgcc attggtgtcg cacagcacca
     2941 gccattgcgc gccgccttcc tgcgcggctt tcaaggcttc gatggcgtag gccggattgg
     3001 ccttgtagcc gtcgaagaaa tgctcggcgt cgaacacggc ctcacggcca ttgctgcgga
     3061 catgggccag cgagtcggcg atcatgcgca ggttttcggc ggtggagacc ttcagcgccg
     3121 tctcgacctg ccagtcccac gtcttgccga caaggcagat gctcggcgtc tgaaccgcca
     3181 gcagatcctg caagcccgga tcgttggagg ccgaacgccc ggcgcggcgc gtcatgccga
     3241 aggccgaaat cctggcggtc tttgtcttcg gcagggcggc gaaaaaggcg tcgtcggtcg
     3301 ggttggcgcc gggccagccg ccttcgatat agtcgatgcc gaacgcgtcc agcgcggagg
     3361 cgatcgcctg cttgtccgcg acggaaaagt cgatgccctg ggcctgcgcg ccgtcgcgca
     3421 gcgtggagtc gtaaagataa acgcgttcgc tcatgcccgc cggtctatat cgccgggaaa
     3481 aacgggtcaa ccgattagct tattgggagg cgttcgcgcc gtccgccttg caggcgtggc
     3541 cgcgcgcttt cgtcacccat aaaggctcga cggtgcggtc gccgtcgcgg aagccgggcg
     3601 cgca
//
LOCUS       DASVLL010000305         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_394448,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000305 DASVLL010000000
VERSION     DASVLL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394448"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="VF402_05145"
     CDS             complement(<1..1663)
                     /locus_tag="VF402_05145"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7799714.1"
                     /translation="MTWFRPADPRTGVLARDHDDVLSSLALVALDLRLQHMARQPDCP
                     FLSASAVSYRVPHTAQAGGLSITPKPGRAKAAFDQAYDALRQFLDKGVTPDEIAVLRS
                     YLTVGMKNYAAGYATRSNDGIADGMIYDLDTDGVPTPLDYNLNIMQRDIDGLDAAEID
                     RRAKIIFSGDGPVLSHSGDHPSDFDEAAMRSAYLAANERKPAAFADDPAKPWPYTDFG
                     PPVAPASRRDIPEFGYRRYVFPNGVKLNVKPTRLAAGQVLVEADFAGGAFRFSPKTTA
                     PVYLLDCGFLMDGGLGKLKAEDINRSLAGRQANVGCATAPDKVILSGAANAADLATEL
                     QIMMAIATDSALSRPVWDRFLSILPQRRRTIDSTPGGVLADRIHEVTAPGDHRVDTAV
                     VDAAPLLDYKRIQDMWRQALTDTPIEITIVGDVDEAAAVAETARTFATLPRRPATANE
                     APDARRTDFPPSRKTYTFYHSGRSDQAIMEVIWPLPGFYADPRQARTQAILTTVLNER
                     LRAVVREQEGQAYDAAAGDWMSPLNDHYGFVWARAGIKAGHEAAYR"
BASE COUNT          262 a          539 c          615 g          293 t
ORIGIN      
        1 ggcgataggc ggcctcgtgt ccggccttga tgccggcccg cgcccagacg aagccgtaat
       61 ggtcgttcag gggcgacatc cagtctccgg cggcggcgtc gtaggcctgc ccctcctgtt
      121 cgcgcaccac ggcgcgcagg cgttcattca gcacggtggt caaaatcgcc tgcgtgcggg
      181 cctggcgcgg atcggcgtag aagccgggca ggggccagat cacctccatg atcgcctgat
      241 cgctgcggcc gctgtgataa aaggtgtagg tcttccgcga cggcgggaag tccgtccggc
      301 gcgcgtcggg cgcttcattg gccgtggcgg gccgccgcgg cagggtggcg aaggtgcgcg
      361 ccgtctccgc gacggccgcc gcttcgtcga cgtcgccgac gatggtgatt tcgatcggcg
      421 tgtcggtcaa ggcctgccgc cacatgtcct ggatgcgctt gtagtcgagc aatggagcgg
      481 cgtcgacgac agccgtatcg acgcgatggt cgccgggcgc ggtgacctca tgaatccggt
      541 ccgccagaac gccgccgggg gtcgaatcga tggtccgccg ccgttgcggc agaatgctca
      601 agaaccggtc ccagaccggg cgcgacaggg cggaatcggt ggcgatggcc atcatgatct
      661 gaagctcggt cgccagatcg gcggcgttgg cggcgccgga taaaatcacc ttgtccggcg
      721 ccgtcgcgca gccgacattg gcctgcctgc ccgccagcga cctgttgata tcctcggcct
      781 tgagcttgcc cagcccgccg tccatgagga agccgcagtc gagcagatag acgggcgcgg
      841 tcgtcttcgg gctgaaacgg aacgcgccgc ccgcgaaatc cgcctcgacc agaacctgac
      901 cggcggccag cctggtcggc ttgacgttca gcttgacgcc gttgggaaag acgtagcggc
      961 gatagccgaa ctccgggatg tcgcgccggc tggcgggcgc gacgggcggg ccgaagtcgg
     1021 tatagggcca cggcttggcc gggtcgtcgg cgaaggcggc cggtttgcgt tcgttcgccg
     1081 ccagataggc ggatctcatc gccgcttcgt cgaaatccga cgggtggtcg cccgaatgcg
     1141 acaggaccgg gccgtcgccg ctgaaaatga ttttggcgcg gcgatcgatt tcggcggcgt
     1201 caaggccgtc gatgtcgcgc tgcatgatgt tcagattgta gtcgagcggc gtcggcacgc
     1261 cgtcggtatc gagatcgtag atcatcccgt cggcgatgcc gtcattgctt cgcgtcgcat
     1321 aaccggcggc gtagtttttc atgccgaccg tcagatagct gcgcagcacg gcgatctcgt
     1381 ccggcgtcac gcccttgtcg aggaactggc gcagcgcgtc ataggcctga tcgaaggccg
     1441 ccttggcccg gcccggcttg ggcgtgatcg acaggccgcc cgcctgcgcc gtatgcggaa
     1501 ccctgtagct gacggcgctg gccgacagaa aggggcagtc gggctgccgg gccatatgtt
     1561 gcaggcgcag atcgagggcg acaagcgcca ggctgctgag gacgtcgtcg tgatcgcggg
     1621 ccaggacgcc cgtgcgcggg tcggcgggac ggaaccaggt cacgcccatt tcttccacaa
     1681 tgccgttgcc ggtgtggctg aaggtggtc
//
LOCUS       DASVLL010000306         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_72562, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000306 DASVLL010000000
VERSION     DASVLL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72562"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1352
                     /gene="mnmA"
                     /locus_tag="VF402_05150"
     CDS             198..1352
                     /gene="mnmA"
                     /locus_tag="VF402_05150"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7799715.1"
                     /translation="MASADFDAALAATRDSVGLPPGARVVAAMSGGVDSTVVAALLHA
                     AGYEVIGVTLQLYDHGAALKKTGACCAGQDIHDARMAAEKIGVPHYVLDYESRFRDSV
                     IEEFADSYLRGETPVPCIRCNQSVKFHDLLQVARDLGAEAMATGHYVERSPEGQLRRA
                     FDLRRDQSYFLFATTREQLDFLRFPLAGIPKPQVRDIARQLGLSIAAKPDSQDICFVP
                     EGKYTTLIDRLRPQGRVAGDIRHVDGRVLGRHDGVTGYTIGQRRGLNIAVGEPLFVTR
                     IDPEAREIVVGPREALLTRSLSLKEINWLGDEPGLTDAARAGKPVLARVRSTRPPVPA
                     RLALNGDNAQVVFDAPEEGVAPGQACVLYDPADESRVLGGGFISGTTAAA"
BASE COUNT          255 a          530 c          481 g          237 t
ORIGIN      
        1 cgcacgccgc atgccataca tttcacgcgg ggcggaccgt ggctgccgca atataagaac
       61 gtgaccttcg gcgagttgtg ggaaaaggaa ttcgccctgc tgcgacaagc gcggtgaaga
      121 cacgataagc ggcgctctgg aaatcggccg cgctttgcat tatctagcgg cgcatggacg
      181 cctgccccgt acccctgatg gcttcggccg atttcgacgc cgccctggcc gccacgcgcg
      241 acagcgtggg gctgccgccc ggcgcgcgcg tcgtcgcggc catgtccggc ggggtcgatt
      301 ccaccgtcgt ggcggcgctg ctgcacgcgg cgggctatga ggtgatcggc gtcaccctgc
      361 aactgtacga tcacggcgcg gcgctgaaga agaccggcgc ctgctgcgcg ggacaggaca
      421 ttcacgacgc gcgcatggcc gccgaaaaga tcggcgtgcc gcactatgtg ctggattacg
      481 aaagccgttt ccgcgattcc gtcatcgagg agttcgccga ttcctacctg cgcggcgaaa
      541 cgcccgtgcc gtgcatccgc tgcaaccaga gcgtcaagtt ccacgacctg ttgcaggtcg
      601 cgcgcgacct cggcgccgag gcgatggcga ccggccacta tgtcgagcgc tcgcctgaag
      661 ggcagttgcg ccgcgccttc gacctccgcc gcgaccagag ctatttcctg ttcgccacca
      721 cgcgcgagca gctcgatttc ctgcgcttcc cgctggccgg catccccaag ccgcaggtgc
      781 gcgacatcgc gcgccaactg ggcctgtcca tcgccgccaa gccggattcg caggacatct
      841 gcttcgtgcc ggaaggcaag tacaccaccc tgatcgaccg cctgcgcccg cagggccgcg
      901 tcgcgggcga catccgccat gtcgacgggc gcgtgctggg ccgccatgac ggcgtcaccg
      961 gctacaccat cggccagcga cgcggcctca atatcgcggt cggcgagcct ttgttcgtca
     1021 cccgcatcga ccccgaggcg cgcgagatcg tcgtcggccc gcgcgaggcg ctgctgacgc
     1081 gcagcctgtc gttgaaggaa atcaactggc tgggcgacga acccggcctg accgacgccg
     1141 cgcgcgcggg caagccggta ctggcgcgcg tccgctcgac ccgcccgccg gtcccggcgc
     1201 gactggccct gaacggcgac aacgcccagg tcgtgttcga cgcccccgaa gaaggcgtcg
     1261 cccccggcca ggcctgcgtc ctctacgacc ccgccgacga aagccgcgta ctcggcggcg
     1321 gcttcatctc cggcacaacc gcagccgcct gagcgtcata ctgggcggct gcgaacccgt
     1381 agcttaaccc gtctcaggtt tggatgtctg gacggtggcc agtaataaag ccgccggaag
     1441 agatgcgatt tcaatggcgc tcaataaaag acagtcccca tctaaatatt cgaaagggct
     1501 tag
//
LOCUS       DASVLL010000307         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_504016,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000307 DASVLL010000000
VERSION     DASVLL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504016"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..637)
                     /locus_tag="VF402_05155"
     CDS             complement(143..637)
                     /locus_tag="VF402_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799716.1"
                     /translation="MTLRLLLILALSCFAFAAAPATHAAARHTAHKTAAKKAPAKKKP
                     EKESREKKKTAARTREKESPKAKTEAALRDQTDSFVALIDAGKQDLTDARIAWGAKRP
                     EVACQRARAAKTRFAKARGVLDGMVKTARDGKADAAPFKALYPKTDELDDATRRLIGT
                     TCAG"
     gene            754..960
                     /locus_tag="VF402_05160"
     CDS             754..960
                     /locus_tag="VF402_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799717.1"
                     /translation="MLDRLEDCCWVEMEGQARPQLVLRRRLKPPVYAVGDWLYAESGS
                     PLAHNPEAPRILLILPPRAHVRRA"
     gene            complement(947..1687)
                     /locus_tag="VF402_05165"
     CDS             complement(947..1687)
                     /locus_tag="VF402_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX7799718.1"
                     /translation="MTETKTAVLYRMVMPAHTCPYGLKSLHLLKSKGYKVEDHWLRTR
                     EETDAFKARHDVRTTPQTFIGGERIGGYDDLRRHFGQRVADPKAKSYTPVLTVFAVTA
                     LLSLVVGWLTGPILSWRTPQWFIGLSMVALAMLKLQNLEKFSTMFLNYDLLAKRWVPY
                     GYIYPFAELLAGLLMTAGALDAVSIPVALFIGTIGAVSVFKAVYIDKRELKCACVGGE
                     SNVPLGFLSLTENLMMILMAVWMLVMRA"
BASE COUNT          268 a          545 c          561 g          337 t
ORIGIN      
        1 ttcaaccatg cgctaccaac atatgtcagg tcttgggcca gtactccaac cttgccaaaa
       61 acgtatccgg gcactgtagg tagttgctga tatttgccga ggcccaggtg tcgccgactg
      121 taaagccggt ttttggggca aatcagcccg cgcaggtcgt cccgatcagc cgccgcgtcg
      181 cgtcgtccag ttcgtcggtc ttggggtaca gcgccttgaa cggcgccgcg tcggccttgc
      241 cgtcccgcgc cgttttgacc atgccgtcca gcacgccgcg cgccttggcg aaccgggttt
      301 tggccgcgcg ggcgcgctgg caggcgactt cgggccgttt ggcgccccag gcgatccggg
      361 cgtcggtcag atcctgcttg ccggcgtcga tcagcgccac gaagctgtcc gtctggtcgc
      421 gcagcgcggc ttcggtcttc gccttgggag actccttctc ccttgttcgg gcggcggttt
      481 ttttcttttc cctggattcc ttttccggct tctttttcgc cggagccttc ttcgcggccg
      541 tcttgtgcgc cgtgtgtcgg gcggcggcgt gcgtcgcggg cgcggcggcg aaagcgaagc
      601 aggacagggc gaggatcagc aggaggcgca gggtcatggc gcgcgaccct aagcggcgcg
      661 cttcgggctg gcaagcgcgc tcgccggatt cgtgatttga ccggcccgcc gtctttggga
      721 acaaagcttg aacgggtatc gggtgagtcg cgtatgctgg atcggctgga agattgctgc
      781 tgggtggaga tggaggggca ggcgcggccg caactggtgc tgcgcaggcg cctgaagccg
      841 ccggtctatg cggtgggcga ctggctctac gccgagagcg gctcgccgct ggcgcacaac
      901 ccggaggcgc cgcgcatcct cctcatcctg ccgccgcgcg cgcatgtcag gcgcgcatga
      961 ccagcatcca caccgccatc aggatcatca tcaggttctc ggtcagcgac agaaaaccga
     1021 gcggcacgtt ggattcgccg ccgacgcacg cgcatttcaa ttcgcgcttg tcgatataga
     1081 cggccttgaa caccgacacg gcgccgatgg ttccgatgaa cagcgccacc gggatcgaca
     1141 cggcgtccag cgcgcccgcc gtcatcagca gccctgccag cagttccgcg aacggataga
     1201 tgtagccgta cggaacccac cgcttggcca gcaggtcgta attcaggaac atggtcgaga
     1261 atttttcgag gttctgcaat ttcagcatgg ccagcgcgac catgctcagc ccgatgaacc
     1321 attgcggcgt gcgccagctc aggatcggcc ccgtcagcca gccgacgacc agcgacaaca
     1381 gcgccgtgac cgcgaacacg gtcagcaccg gcgtatagga cttggccttc gggtcggcga
     1441 cgcgctggcc gaaatggcgg cgcagatcgt catagccgcc gatgcgttcg ccgccgatga
     1501 aggtctgcgg cgtggtcctg acgtcatgcc gggccttgaa ggcgtcggtt tcctcgcgcg
     1561 tcctcagcca gtgatcctcg accttgtagc ccttgctttt cagcaggtgc agcgatttca
     1621 ggccataggg gcaggtgtgc gccggcatca ccatgcggta gaggacggcg gttttcgtct
     1681 cggtcatcgt cgggccttcc ttgaatttga c
//
LOCUS       DASVLL010000308         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_504019,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000308 DASVLL010000000
VERSION     DASVLL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504019"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1025)
                     /locus_tag="VF402_05170"
     CDS             complement(144..1025)
                     /locus_tag="VF402_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX7799719.1"
                     /translation="MIKINRLFLLRLIPTVIVVAAALFVARGLWRHYEEEPWTRDGRV
                     RADVVEVAPDVSGLITAVAVRDNQAVKAGDLLFTVDQPRYRLAVEQAQAAILAQQPAL
                     REAKQEAARNHALGDLVAAEVTQAADAKVAALQAALAQSRVALDAAQLNLSRTEVRAP
                     VDGVIANLSLRPGNYAAAGRPEFALLASNSVYIVGYFEETKLKRIHLGDKVKARLMGD
                     DAAIDGHVESIAGGVDERDGGAGASLLANVNPTFSWVRLAQRIPVRVHIDRVPQGVAL
                     VAGRTATVEVVAPEVKK"
     gene            complement(1036..1239)
                     /locus_tag="VF402_05175"
     CDS             complement(1036..1239)
                     /locus_tag="VF402_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEX7799720.1"
                     /translation="MTPELNLSGVYVHPLLAAAVLAFIAGRLIELLLARLRLYRFIWH
                     RGLFDVAMSVILWGAFARLLTLN"
     gene            1275..1393
                     /locus_tag="VF402_05180"
     tRNA            1275..1393
                     /locus_tag="VF402_05180"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Asp,seq:gtc)
BASE COUNT          397 a          830 c          906 g          323 t
ORIGIN      
        1 cgcgaaggcc ggattgtcgc cgttcaggaa ggcgccctgc gcggcgggat tggcgatctg
       61 cggatgcgct tcggggacgt gcgcgggaac ggcggcgcag gccgacaggg ccagcagcga
      121 ggcgccgatg agcaggttgc ggatcatttt ttcacctccg gcgcgacgac ctcgaccgtg
      181 gccgtgcggc ccgcgaccag cgccacgcct tgcggaaccc tgtcgatatg gacgcgcacc
      241 gggatgcgct gcgccagccg cacccacgag aaggtcgggt tgacattggc cagcagcgac
      301 gcgcccgcgc cgccgtcgcg ctcatcgacg ccgcccgcta tgctctcgac atggccgtcg
      361 atcgccgcgt cgtcgcccat cagccgcgcc ttgaccttgt cgccaagatg gatgcgcttg
      421 agcttcgttt cctcaaaata tccaacaata tagacggaat tgctcgcaag cagcgcgaat
      481 tcgggccgtc ccgccgccgc gtagttgccg gggcgcagcg acaggttggc gatcacgccg
      541 tcgaccggcg cgcgcacctc ggtgcggctc agattcagtt gcgcggcgtc gagcgcgacg
      601 cgcgattgcg ccagcgcggc ctgcaaggcg gcgaccttgg cgtcggccgc ttgtgtgacc
      661 tcggcggcga ccagatcgcc gagcgcgtgg ttgcgcgcgg cctcctgctt cgcctcgcgc
      721 agggcgggct gctgggcaag gatggcggcc tgcgcctgct cgacggcgag gcggtagcgc
      781 ggctgatcga cggtgaacag gaggtcgccc gccttgaccg cctgattgtc gcgcacggcg
      841 acggcggtga tcaggccgga aacgtcgggc gcgacctcga ccacgtcggc gcgcacgcgg
      901 ccgtcgcgcg tccacggctc ctcctcgtaa tgccgccaca ggccgcgcgc cacgaacagg
      961 gcggcggcga cgacgatgac ggtcgggatc aggcggagca ggaataaacg gttgattttg
     1021 atcatgacgc acacatcagt tcaaggtaag aagccgggcg aacgcgcccc acaggatcac
     1081 gctcatggcg acgtcgaaca ggccgcgatg ccagatgaag cggtagagcc gcagccgcgc
     1141 cagcagcagc tcgatcagcc gcccggcgat gaaggccagc acggcggcgg ccagcagcgg
     1201 atggacgtaa acgccggaca ggttcagttc gggggtcatc gggcggcctc catgcgcggc
     1261 gcttcggcga gggggaacag gttgcggcgc aggccggtca gggcgaggcg cgtatcgagg
     1321 ccgcgtgttt cgcgcagcgc gccgtcgata ttgtcgcgca gggagggcga gggttcgact
     1381 ccctctctgt ccgccgtcgc cgcgtaatgt ccggccacgg cgtcgagcag ggcgtcgagc
     1441 cggtcgcgcg cttcgggcgt gtcgcgcagc gtgatcaggt tgatgccgac gcgcaggtcg
     1501 cgcagcgcgt ccgccgccgc cagccgctca tgcccgcgcg acgccgccag acgcggcgcc
     1561 agcagttgcg cgcggtccag catcaggccg gtccagtcgc tgacccccgc tttgaggcgg
     1621 cgcgcggcga tggcgctgag gtcgcgccag cccgcgcgca gcacgcgctg cgccgccgtc
     1681 tgcgggccga tgacgcggat cagccgcgtg gcgatcaccg cgccgacaat gcccgcgacc
     1741 tgcgcgaaat tggcgttggc gaaggcggcg aaatcggcgg aaaacgtgtt ggtcagcgcc
     1801 agccccatgc agaagccgag cagcagcggc atgatgcgcg catagaggac cgggcgcggc
     1861 agcagcacgc cgaggaccag cagcaccggc gccatcgcca gacacagggt gacgaagtcg
     1921 tgcgcggcgg gcaggacggc gaactgatac agcgccacca gcggcagcga ggcgatggtc
     1981 cacagcatga agccgcgctg cgacggcgtc ggatcgtcca tcgcgccgta gaagcagcac
     2041 atgaccgccg cgatcatcgg cgcgatggcg ccttcgggcc agccggtgac gatccacgcc
     2101 gcgcaggcgc cggtgacggc gaggaaggtc gccgcgaccg acaaaaacgc catgccgagg
     2161 tcgatgtgca gcgggcgctt gaggcggcgg cggaccagat cctgcgagtc cggcaacaga
     2221 acatgtttcg catcgctcag gtgcaggctg aggtcgcgcc cggcgcgcaa aagcgccatc
     2281 aactccttca ggcgcgcgat caggttcagc gtcagcagcg ggcgccagcc gtcgtcgggc
     2341 gttgcggcgg ccagcgcgtc gaggctggcg cgcgcggcgg cgggttcgat catgcggtcg
     2401 ccctcgaccc agaggcgcac ggcgtcgagg cgcgcctgca cggcgggcgt caggcc
//
LOCUS       DASVLL010000309         4345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_449805,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000309 DASVLL010000000
VERSION     DASVLL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449805"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..3093
                     /locus_tag="VF402_05185"
     CDS             124..3093
                     /locus_tag="VF402_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799721.1"
                     /translation="MKTKSHNAIFASTRRVSLLAGASFGVLLSLAAGGALAQDAQPAA
                     SAPASANDDSTVVIVTGFRGSLQSALSAKRKEDSIVDVIKSEDIAQFPDNNLAEALQR
                     IPGVTIDRDGGEGRTITVRGLGPDFTRVRLNGMEALATTGGKDSSGGANRRRGFDFSV
                     FAAELFQQVTVRKSPEAATEEGSLGATVDLQAPHPFDYKGFAMGGALQEGYNDLSKSS
                     APRGSFLVSNRWADGKLGALFSVAYSDKTEFEEGPSTTRWENAYSASSVMRFAGYSAD
                     GGSTFTAIPECAKAACPAGSGTPVLTDPQAIAISNALHPRIPRYGRLTYHQKRLGMTG
                     SFQMKPNPDTLITIDALYSDFIADRDEQYLEAISFSRSGAGLPKTTVYDYTIDDNQDL
                     IKGTFNNVDIRTENRHDNLETQFGQLALTWDQNIGDRLKLKGYLGASRSTQMNPEQTT
                     LSFDAYDVQGYSYDFTDQQHPVFNYGTSNGCTVDQACYWQMSDSTAKGDASLVRLRPN
                     TTKNNFGTASLSAIYDFDNGFKLEAGAQAKKYSFSTTQYWLADSTEPLPKANGDAASK
                     LLNADLASYAQPITAFGVTWLMPDLSKIDDAIHYNCDCVNQYGDFRLSTTTTKAAPYN
                     QAATERDGSSFIQADFNRDLFGINVRGNLGVRYVTTDQTVNGIQVNKAGTQFTQITIN
                     RKYSDTLPALNIVAEPIDNLLVRFAASKVMARPTMQSLVPNGTLDLTAGTPTWSGGNP
                     NLNPIRATDYDFNVEWYPNKDALISGGVFYKDITSYIQTLSYTGTLGDLGLTQEQIDA
                     QGGSGLTPDSSATIKNPINTPGGILRGWEFNIQDNFSFLPWYFKNLGGIFNYTHVSSQ
                     ISYYTTGSSTSGVIKANLLGMSPNSWNATLYYQDSRFEARLTASHRDKYLQLLNPGSS
                     AAFQGKNAITDIDAQLTYSLNKHLTLTFQGINLTNVPDSDSFDYYDTAQGNTDPAAML
                     EYLKDGRQYYVGIRYKY"
     gene            complement(3237..>4345)
                     /gene="pelA"
                     /locus_tag="VF402_05190"
     CDS             complement(3237..>4345)
                     /gene="pelA"
                     /locus_tag="VF402_05190"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX7799722.1"
                     /translation="DKAALAAERRGLAQIPPPPQEGHGETMPLDRPDAWYATAQARHV
                     ADVIVSFQTPAGGWGKNQRRDGAPRKPGQAYVADNISKFLSPGDYDIPHDPRWNYVGT
                     LDNNATTTEMRFLVRVAAQAPGPEGDAYRASFLRGVRYLLDAQYPNGGWPQVWPLEGG
                     YHDGVTYNDNAVALAAMTLSAAAVGRGDYAFVPRDVRAAAGEAAAKAVWVILATQVRS
                     GGVATIWAQQYDPLTLTPESARNFEPPALASDESADVLIFLMQQPDPSPAVVAAVHDA
                     VAWLKAHALRDVAWTRTDRGRELVPSPGAGPIWARYYDAGTGRPVFGDRDKSLHDDVA
                     DLSLERRNGYNWFDTSPQKALDAYAAWAKAHPGG"
BASE COUNT          792 a         1559 c         1286 g          708 t
ORIGIN      
        1 tagccaaaac gaacgcgccc ggagccatcc agtgcgtccg tccttagctg aacccataat
       61 tgaaaaggtc tgccggaagc gcgtttctgt tccggcgtcg aggccaaaac ggggagtgat
      121 tttatgaaaa ccaagtctca caacgccata tttgcgtcca cccgccgcgt gtccctgctg
      181 gccggcgcct ccttcggcgt gctgttgtcg ctggccgccg gcggcgcgct ggcgcaggac
      241 gcgcaaccgg cggcttccgc ccccgcctcc gcgaacgacg attccaccgt cgtcatcgtc
      301 accggctttc gcggttcgct gcaatcggcc ctgtcggcca agcgcaagga agactcgatc
      361 gtcgacgtga tcaagtccga ggacatcgcg caattcccgg acaacaatct ggccgaagcg
      421 ctgcaacgca ttccgggcgt caccatcgac cgcgacggcg gcgaaggccg caccatcacg
      481 gtgcgcggcc tgggccccga cttcacccgc gtgcgcctga acggcatgga ggctctggcc
      541 accacgggcg gcaaggattc gtccggcggc gccaaccgca ggcgcggctt cgacttctcg
      601 gtcttcgccg ccgagctgtt ccagcaggtg acggtgcgca agtcgcccga agcggccacc
      661 gaagaaggca gcctgggcgc cacggtcgat ttgcaggcgc cgcatccctt cgactacaag
      721 ggcttcgcca tgggcggcgc cttgcaggaa ggctataacg acctgtcgaa atcctcggcg
      781 ccgcgcggct cgttcctggt gtcgaaccgc tgggccgacg gcaagctggg cgccctgttc
      841 tcggtcgcct attccgacaa gaccgaattc gaggaaggcc cgtcgacgac gcgctgggaa
      901 aacgcctaca gcgcctcgtc ggtcatgcgc ttcgccggtt actccgccga cggcggctcg
      961 accttcaccg ccattcccga atgcgccaag gccgcctgcc ccgccggttc cggcacgccg
     1021 gtgctgaccg acccccaggc gatcgcgatc tccaacgcgc tgcacccgcg cattccgcgc
     1081 tacggccgcc tgacctatca tcagaaacgc ctcggcatga ccggctcgtt ccagatgaag
     1141 ccgaatcccg acacgctgat caccatcgac gcgctgtatt ccgacttcat cgccgaccgc
     1201 gacgagcaat atctggaagc catctcgttc agccgctccg gcgccggcct gcccaagacg
     1261 accgtgtacg actacacgat cgacgacaat caggatctga tcaagggcac gttcaacaat
     1321 gtcgatatcc gcacggagaa ccggcacgac aatctggaga cccagttcgg tcaactggcc
     1381 ctgacctggg accagaacat cggcgaccgc ctgaagctca agggctatct gggcgcctcg
     1441 cgctcgacgc agatgaaccc ggagcagacc accctgtcct tcgacgccta tgacgtgcag
     1501 ggctattcct acgacttcac cgaccagcag cacccggtgt tcaactacgg caccagcaac
     1561 ggctgcaccg tcgatcaggc ctgctactgg cagatgtcgg attcgaccgc caagggcgac
     1621 gcctcgctgg tgcgcctgcg tccgaacacg acgaaaaaca atttcggcac ggcctcgctg
     1681 agcgccatct acgacttcga caacggcttc aagctggaag cgggcgcgca ggccaagaag
     1741 tacagcttct cgaccacgca atactggctg gcggactcga ccgagcctct gcccaaggcc
     1801 aatggcgacg cggcctccaa gctgctcaac gccgatctgg cctcctacgc ccagccgatc
     1861 accgccttcg gcgtgacctg gctgatgccg gacctcagca agatcgacga cgcgatccat
     1921 tataactgcg actgcgtcaa ccagtacggc gacttccggc tgtcgaccac gaccaccaag
     1981 gccgcgccct acaatcaggc ggccaccgaa cgcgacggct ccagcttcat tcaggccgac
     2041 ttcaaccgcg acctgttcgg gatcaatgtt cgcggcaatc tgggcgtgcg ctacgtgacg
     2101 accgaccaga ccgtcaacgg cattcaggtc aacaaggccg gcacccagtt cacgcagatc
     2161 acgatcaacc gcaagtacag cgacaccctg cccgccctga acatcgtcgc cgagccgatc
     2221 gacaacctgc tggtgcgctt cgccgcgtcc aaggtgatgg cgcgtccgac catgcagtcg
     2281 ctggtgccga acggcacgct ggacctgacg gcgggcacgc cgacctggtc gggcggcaat
     2341 cccaacctga acccgatccg cgccacggat tacgacttca acgtcgaatg gtatccgaac
     2401 aaggacgcgc tgatttcggg cggcgtcttc tataaggaca tcacctccta catccagacc
     2461 ctgtcctata ccggcacgct gggcgatctg ggcctgacgc aggagcagat cgacgcgcag
     2521 ggcggctccg gcctgacgcc ggattcgtcg gccacgatca agaacccgat caacacgccg
     2581 ggcggcatcc tgcgcggctg ggagttcaac attcaggaca atttcagctt cctgccgtgg
     2641 tacttcaaga atctgggcgg catcttcaac tacacccacg tctcgtcgca gatcagctac
     2701 tacaccacgg gctcctcgac ctccggcgtg atcaaggcca acctgctggg catgtcgccc
     2761 aattcgtgga acgcgaccct ctactatcag gacagccggt tcgaagcccg cctgaccgcc
     2821 tcgcaccgcg acaagtacct gcaactgctc aaccccggct ccagcgccgc cttccagggc
     2881 aagaacgcga tcaccgacat cgacgcgcaa ctgacctaca gcctgaacaa gcacctgacc
     2941 ctgaccttcc agggcatcaa cctgacaaac gtgccggaca gcgactcgtt cgactattac
     3001 gacaccgcgc agggcaacac cgacccggcc gcgatgctgg aatacctcaa ggacggccgc
     3061 caatactatg tcggcatccg ctacaagtac tgatctttcc cttcctccac acttcgcagg
     3121 cgctgctttc gggcagcgcc ttttttttaa gcgggcgaca ggagatacag gactcacacg
     3181 tttgcgagcg cgcgcatcat gttcgacgcg acgatctgcg agacgatcgc gtaagcctat
     3241 ccgcccggat gcgccttcgc ccatgcggcg taggcgtcca gcgctttttg cggcgatgtg
     3301 tcgaaccagt tgtagccgtt gcggcgttcg aggctgaggt cggccacgtc gtcgtgcagg
     3361 ctcttgtcgc ggtcgccgaa caccggcctg cccgtcccgg cgtcgtaata gcgcgcccag
     3421 atcggcccgg cgcccggcga cgggaccagc tcgcggcccc gatccgtgcg cgtccacgcc
     3481 acgtcgcgca gcgcgtgcgc cttcagccac gccacggcgt catgcaccgc cgcgaccacg
     3541 gcgggcgacg gatcgggctg ctgcatcagg aagatcagca cgtccgcgct ttcgtcgctg
     3601 gccagggcgg gcggctcgaa attgcgcgcc gattccggcg tcagggtcag cggatcgtac
     3661 tgctgcgccc agatcgtcgc gacgccgccg gacctgacct gcgtggcgag gatgacccac
     3721 accgccttcg ccgccgcttc gcccgccgcc gcgcgcacgt cccgcggcac gaaggcgtag
     3781 tcgccgcgcc cgaccgccgc cgcgctcagc gtcatcgccg ccagcgccac cgcgttgtcg
     3841 ttataggtca cgccgtcatg atagccgcct tccagcggcc agacctgcgg ccagccgccg
     3901 ttcggatact gggcgtccag cagatagcgc acgcccctga ggaagctggc gcggtacgcg
     3961 tcgccctccg gtcccggcgc ctgcgcggcg acccggacca gaaagcgcat ctcggtcgtg
     4021 gtcgcgttgt tgtcgagcgt gccgacatag ttccaccgcg gatcgtgcgg aatgtcgtaa
     4081 tcgccggggc tgaggaattt cgagatgttg tcggcgacat aggcctgccc cggcttgcgc
     4141 ggggcgccgt cgcggcgctg gttcttgccc cagccgcccg ccggggtctg gaacgagacg
     4201 atcacgtccg cgacatgccg cgcctgcgcc gtcgcgtacc acgcatcggg ccggtccagc
     4261 ggcatggtct caccgtggcc ttcttgcggc ggcggcggaa tctgcgccag cccgcgccgt
     4321 tcggcggcca gggcggcctt gtcgg
//
LOCUS       DASVLL010000310         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_341040,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000310 DASVLL010000000
VERSION     DASVLL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341040"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VF402_05195"
     CDS             complement(<1..263)
                     /locus_tag="VF402_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX7799723.1"
                     /translation="MEHGPLASIAILGGGTAGWMTAAALVRRLAGRCRVELIESQEIG
                     IVGVGEATLPHLRAFNEALGIDEAEFMAATNATFKLGIEFVDWG"
     gene            complement(494..>2312)
                     /locus_tag="VF402_05200"
     CDS             complement(494..>2312)
                     /locus_tag="VF402_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799724.1"
                     /translation="SNGITGSEGGCYLNPNPGGYGYNPQLHVDTTGSTLAFSGFEDAM
                     LGVGGLGAGATTADYMKNLDSYKVAAYSSEGNNDARSDLNVARVDGHYRFDSKLFGLF
                     TKLDGGVRLSDRTVSIEQFHLFSNFYGGTGVQMSGDPVAQAGCAAQWKAIDVVMNNPI
                     CSAGEFVPSADPAAAPGATVFQGYTVNRPTALDEHNPVIFVTGLGGKTASGIPGFWAV
                     DPKAFDNEEAFMKSVFGGAQRVSVPGQSYDVELKEDSAFLSGEFETGIFSGNVGVHVI
                     NTKLRVRQNLTDPTAVPYGDDNFDDGDTVSERSYTDVLPSLNLNADVTENFKLRFAAS
                     KTMQPLDLGNYGGGVSIATHDNQLLNRREVTSVSFGGNPDLPPWRSSNFDFSAEYYLG
                     KASMVNLALFDLEIESFVTGGTALSQPGDYQNSDGSPAGPVPVSLPIAGHGGQVSGLE
                     VGGKFAFKDFLSSGLLSNFGVDANYTLSPSHGQAKGLDGERLPFIDNSKHQFNLIGWY
                     QDKRLQVRVAYNYRSERFSQLNGLTAGSNVPIFAEPTNYVDVNATWNVNDRVSVYVNG
                     SNVTGEVEKYYYHFGDASKQYAYQNEFEPRYTLGVRAKW"
BASE COUNT          400 a          727 c          772 g          413 t
ORIGIN      
        1 ccccagtcga cgaactcgat gccgagcttg aaggtggcat tggtcgccgc catgaactcg
       61 gcctcgtcga tccccagcgc ctcgttgaac gcgcgcagat gcggcagcgt cgcctcgccg
      121 acgccgacga tgccgatctc ctgcgattcg atcagctcga cgcggcagcg ccccgccagc
      181 cgccgcacca gcgccgccgc cgtcatccag ccggcggttc cgccgcccaa tatggcgatg
      241 gaggcgagcg ggccatgctc catgtgatgc gtcaccggtg tcttccgttt cttgtcatgc
      301 ggctcctccg atgcggagcc gcgtgagaaa aggcggagga gaggaaaagg cctcccggca
      361 agaccttaaa ccggtttcga cgggaggcaa gacgatgctc cggggaagcc cccggaacag
      421 ccagttgggg gaagaacgga ccggcgccaa cgccggcccg ctcatgacat ggcttgaaaa
      481 gccatgttgg cggctaccac ttggcgcgca cgcccagcgt gtaacgcggc tcgaactcgt
      541 tctgatacgc gtactgcttc gaggcgtcgc cgaagtggta atagtatttc tcgacctcgc
      601 cggtgacgtt ggagccgttg acatagacgc tgacgcggtc gttgacgttc caggtcgcgt
      661 tgacgtcgac atagttggtc ggctcggcga agatcggcac gttgctgccc gccgtcaggc
      721 cgttcagctg gctgaaacgc tccgaacggt agttgtaggc gacgcgcacc tgcaggcgct
      781 tgtcctgata ccagccgatc aggttgaact ggtgcttcga gttgtcgatg aacggcaggc
      841 gctcgccgtc cagccccttg gcctgaccgt ggctcggcga cagggtgtag ttggcgtcga
      901 cgccgaagtt ggacagaagg ccgctgctca ggaagtcctt gaaggcgaac ttgccgccga
      961 cctccagacc cgacacctga ccgccgtgcc cggcgatggg cagcgacacc ggcacgggac
     1021 cggcgggcga accgtcggag ttctggtaat cgccgggctg gctcagcgcc gtgccgccgg
     1081 tgacgaagct ttcgatctcg agatcgaaca gcgccaggtt caccatcgag gccttgccga
     1141 gatagtattc cgccgagaag tcgaaattgc tcgaacgcca gggcggcagg tccggattgc
     1201 cgccgaagct gaccgaggtc acttcgcggc ggttcaggag ctggttgtcg tgcgtggcga
     1261 tggagacgcc gccgccgtaa ttgcccagat ccaacggctg catggtcttg gacgcggcga
     1321 agcgcagctt gaagttctcc gtcacgtcgg cgttcaggtt caggctgggc agcacgtcgg
     1381 tgtagctgcg ttccgacacg gtgtcgccgt cgtcgaaatt gtcgtcgcca tacggcacag
     1441 cggtcggatc ggtcaggttc tggcgcacgc gcagcttggt gttgatgacg tggacgccga
     1501 cattgccgga gaagatgccc gtctcgaatt cgccggacag gaaggccgag tcttccttga
     1561 gttcgacgtc atacgattgt cccggcacgc tgacgcgctg ggcgccgccg aacacgctct
     1621 tcatgaaggc ttcctcgttg tcgaacgcct tgggatcgac agcccagaag ccgggtatgc
     1681 ccgaagcggt cttgccgccg aggccggtca cgaagatgac cgggttgtgt tcgtccagcg
     1741 cggtcggacg gttgaccgta taaccctgga acaccgtggc gcccggcgcg gcggccgggt
     1801 cggcggacgg cacgaactcg cccgccgagc agatcgggtt gttcatcacc acgtcgatcg
     1861 ccttccactg cgccgcgcag cccgcctgcg cgaccggatc gccgctcatc tgcacgcccg
     1921 tgccgccgta gaagttggag aacaggtgga actgctcgat gctgaccgtg cggtcggaca
     1981 ggcgcacccc accgtccagc ttggtgaaca ggccgaacag cttgctgtcg aaacggtaat
     2041 ggccgtcgac gcgcgcgacg ttcaggtcgg agcgggcgtc gttattgcct tccgacgaat
     2101 aggccgcgac cttgtaggaa tcgaggttct tcatgtagtc ggcggtggtc gccccggcgc
     2161 cgaggccgcc cacgcccagc atcgcgtcct cgaagccgga gaaggccagg gtcgaaccgg
     2221 tggtgtcgac gtgcagttgc ggattatagc cgtaaccgcc cggattgggg ttcaggtagc
     2281 agccgccttc cgagccggta atgccgttcg ag
//
LOCUS       DASVLL010000311         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_288781,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000311 DASVLL010000000
VERSION     DASVLL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288781"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VF402_05205"
     CDS             <1..529
                     /locus_tag="VF402_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7799725.1"
                     /translation="VNNAGVTGNAQDGQNDPEHTTLDEWRRVTRVNLDGVFLGCKHAI
                     RVMRPYGGAIINMSSRSGLVGVPHQAAYAASKAAVRNHTKSVALYCAEQGLNIRCNSV
                     HPAAILTPMWEAMLKDGADAEAVANASPLHRFGRPDEVAAIVTLLASDEATFITGSEF
                     NIDGGILAGSAGAPK"
     gene            complement(594..1514)
                     /locus_tag="VF402_05210"
     CDS             complement(594..1514)
                     /locus_tag="VF402_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7799726.1"
                     /translation="MSETLSRVAVFFAFIALGAVLVRFKRLDRNGLNGLTAYFYWLAF
                     PCWLLVGFSRLPPLDAAHARVLLVYLAAMLAAAAIAVLLMRLSGGDRARSVAAGMAAM
                     VNNSAFLGAPVAASLFGPKVMIMAPLVVAADFLVLFSLGCAGLAKASGHTFREALIHT
                     AKNPTVIGAALGVALMSFGVRFPPVLDGALDLLGKSGPPVALVALGGMLGLMPASRLI
                     RPDRLSVFALLGKLLLAPALVALALHLLRADPLTFRVCVFLSATPTAVSVFVQTRMYD
                     FWSEGAARTVAQGTLISLFTLCGLALLLTA"
     gene            1673..3010
                     /locus_tag="VF402_05215"
     CDS             1673..3010
                     /locus_tag="VF402_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799727.1"
                     /translation="MFEFGRELRRIFRQHDRIDADASLYELMNLQMLIAQGRQFDIEG
                     GRVSTRDRFTPYIQAALIWREYARRTGDPVAVRRAAASAENAGKAAQSAQEAAQAALE
                     QALTCLLSHDLFETGDLLSSAEDLLATGRAATPGDESLRARYNRAEAQLAMRLAVRQG
                     VGEDLELAMLAMSHIDRAVERADQRVLKTHAGADKIEAALARLMRADLLMLVGMDRGD
                     ASLMAAVIKDFEALRARLDPAYAPVTHARVTLRLAQAHIGRGEIEGRPEAVSEGVALL
                     SRDDAIFDYEHAPLDWVAHQQALAAGLQVLGELTLNADLHEKAMAVYDLALKRPLQKG
                     LSMRARLIEARAACLTRHAESQGSLKALDVAEAAFKTELRAIRAHDDPVAWAILQWQL
                     ARIYVARGEISGFMMERAEAAYALEAALDIFREQGLDAMMATASALQDSIRAL"
     gene            3102..4751
                     /locus_tag="VF402_05220"
     CDS             3102..4751
                     /locus_tag="VF402_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX7799728.1"
                     /translation="MTQIHRRHVLASVAGLGLAASARAQTLVTSTQIAGPFVEIETAG
                     GRLRGGHERGAIAFKGVPYAGSVSGANRFKAPPRAPSWAGVRDALRLGPPAPQTPGST
                     YGEQEPGRSEDCLVLNVWTPAVNDGRKRPVMVYFHGGGYSTGSAGSPTQDGARLAALY
                     DVVVVAPNHRLGLLGFLWLGDLLGADYATSGNQSMFDMIASLQWVRDNIAAFGGDPGN
                     VTIFGESGGGAKVGTLMGMPAAKGLFHKAGIQSGAQLRRMTRATAAETAQRLMRALDL
                     SDARRLHEVPVETLLKLQWDSEAAPQAVKPEMTGISFSESTLPGHYGPVVDGWALPAD
                     PFDPAASDLCADIPLLIASNKTEAAFFFMNDPAAFALDAAGLTRLLRDNLGRYADTVL
                     QTYRALMPKATPSELFLAIATARTMGIETIVLADRKAAQAAPVYRYRWDYASNLPVAG
                     TSATLGAGHATDIGPTFANFDEKGLHGNGPGVKAASLNLSAIWAGFAHTGVARAPGVA
                     DWPRYTPQTRATVLVDEKCVLADDPDAAAREMWMKIDPRWG"
BASE COUNT          823 a         1510 c         1776 g          690 t
ORIGIN      
        1 ggtcaacaac gccggggtca ccggcaacgc gcaggacggc cagaacgatc ccgaacatac
       61 gacgctggac gaatggcggc gcgtgacgcg cgtcaatctg gacggcgtgt tcctgggctg
      121 caagcacgcg atccgggtca tgcgccccta tggcggggcc atcatcaaca tgtcgtcgcg
      181 gtcgggattg gtcggcgtgc cgcatcaggc ggcctatgcg gcgtccaagg cggcggtgcg
      241 caaccacacc aagagcgtgg cgctgtactg cgccgagcag gggctgaaca tccgctgcaa
      301 ttcggtgcat ccggcggcga tcctgacgcc gatgtgggag gcgatgctga aggacggcgc
      361 cgacgccgag gccgtggcga acgccagccc gctgcaccgc ttcggacggc ccgacgaggt
      421 ggcggccatc gtgacgctgc tggcctcgga cgaggcgacc ttcatcaccg gttcggaatt
      481 caacatcgac ggcggcatac tggccgggtc ggcgggcgcg ccgaaataga gcgtttcccc
      541 aaaaagtcgc tccacttttt ggataaggaa gagcgacaaa caaaaacatc taatcacgct
      601 gtcagcagca gggccagtcc gcacagggtg aacaggctga tcagcgtgcc ctgcgccacc
      661 gtcctggccg cgccttcgct ccagaaatcg tacatgcgcg tctgcacgaa cacgctgacg
      721 gcggtgggcg tggcgctgag gaacacgcag acgcggaagg tcagcggatc ggcgcgcagc
      781 agatgcaggg ccagcgcgac cagcgccggg gccagcagca gcttgccgag gagggcgaaa
      841 acgctgagcc ggtcgggacg gatcagacgc gatgcgggca tcaggcccag catgccgccc
      901 agcgcgacca gcgccacggg cggcccggac ttgcccaaaa ggtcgagcgc gccgtccaga
      961 acgggcggaa aacggacgcc gaagctcatc agcgcgacgc cgagcgcggc gccgatcacg
     1021 gtcggattct tcgccgtgtg gatgagcgcc tcgcggaagg tatggccgga cgccttggcc
     1081 agccccgcgc agccgaggct gaacagcacg aggaagtcgg cggcgacgac cagcggcgcc
     1141 atgatcatga ccttcgggcc gaacaggctg gcggccaccg gcgcgccgag gaaggcggaa
     1201 ttgttgacca tcgcggccat gcccgccgcg acgctgcgcg cccggtcgcc gccggacagg
     1261 cgcatgagca ggacggcgat ggcggcggcg gccagcatgg cggccagata gacgagcagc
     1321 acccgcgcat gcgccgcgtc cagcggcggc aggcgcgaaa agccgaccag cagccagcac
     1381 ggaaaggcca gccagtaaaa ataggcggtc agcccattga ggccgttccg gtccagccgc
     1441 ttgaagcgga ccagaacggc gccgagggcg atgaaggcga aaaatacggc gacgcgggac
     1501 agggtttccg acatgcggcc tgaatgcgct gaaaatccgt tgtggcgcaa gcttgatctt
     1561 aaactgtgga cgaaatcgcg cgggcggcgc ggcgtttgca ttgccgcctg cgaaggctga
     1621 cgaagcgtat cgtcctgaca cgctgccgat ccgccgaacg gaccagatca gtatgtttga
     1681 attcggacgc gaattgcggc gcatcttccg ccagcatgat cggatcgacg ccgacgccag
     1741 cctgtatgag ctgatgaatc tgcaaatgct gatcgcgcag gggcgtcagt tcgacatcga
     1801 aggcggacgg gtgtcgacgc gcgaccggtt cacgccctac attcaggcgg cgctgatctg
     1861 gcgcgaatat gcgcggcgca ccggcgatcc ggtggcggtg cggcgcgcgg cggcctcggc
     1921 ggagaatgcg ggcaaggcgg cgcagtcggc gcaggaggcg gcgcaggccg cgctggagca
     1981 ggcgctgacc tgcctgttga gccacgacct gttcgagacg ggcgatcttt tgtcgtcggc
     2041 ggaggatctg ctggcgacgg gccgggcggc gacgccgggc gacgagtcct tgcgcgcgcg
     2101 ctacaaccgc gccgaggcgc aactggcgat gcggctggcc gtgcgtcagg gcgtgggcga
     2161 ggatctcgaa ctggccatgc tggccatgtc gcacatcgac cgcgcggtcg agcgcgcgga
     2221 tcagcgcgtg ctgaaaaccc atgccggggc ggacaagatc gaggcggcgc tggcgcggct
     2281 gatgcgcgcc gacctcctga tgctggtcgg gatggatcgc ggcgacgcca gcctgatggc
     2341 ggcggtgatc aaggatttcg aggcgctgcg ggcgcggctg gacccggcct atgcgccggt
     2401 cacccatgcg cgcgtcacgc tgcggctggc gcaggcgcat atcggccggg gcgagatcga
     2461 gggccgcccc gaggcggtca gcgagggcgt ggcgctgttg tcgcgcgacg acgccatctt
     2521 cgattacgaa cacgcgccgc tggactgggt cgcgcatcag caggcgctgg cggcgggctt
     2581 gcaggttctg ggcgaactga cgctgaacgc ggacctgcac gaaaaggcga tggcggtgta
     2641 cgatctggcg ctgaaacggc cgctgcaaaa ggggctgtcc atgcgcgcgc ggctgatcga
     2701 ggcgcgcgcc gcctgtctga cgcggcacgc cgaaagccag ggcagcctga aagcgctcga
     2761 cgtcgccgag gcggcgttca agacggaact gcgcgccatc cgcgcgcatg acgatccggt
     2821 ggcgtgggcg atcctgcaat ggcaactggc gcgcatctat gtggcgcgcg gcgagatcag
     2881 cggcttcatg atggagcgcg ccgaggcggc ctacgcgctg gaggcggcgc tggatatttt
     2941 ccgcgagcag gggctggacg ccatgatggc gacggcgtcg gccttgcagg acagcatccg
     3001 ggcgctttga ccaacatctg ataacgctaa caatcgcctt gccgcgcggc gaggctgggc
     3061 gtagcgtgcc tgcataacca agatatgcag ggatattcgc catgacgcag attcaccgcc
     3121 ggcatgtgct ggcctccgtc gccgggctgg gcctggccgc ttcggcgcgc gcgcagacgc
     3181 tcgtcacatc gacccaaatc gcggggccgt tcgtcgagat cgagaccgcc ggcgggcggc
     3241 tgcgcggcgg ccatgagcgc ggcgccatcg ccttcaaggg cgtgccctat gccggttcgg
     3301 tgtcgggcgc caaccggttc aaggcgccgc ccagggcgcc gtcgtgggcc ggggtgcgcg
     3361 acgcgctgcg gcttggtccg cccgcgccgc agacgccggg ctcgacctat ggcgagcagg
     3421 agccggggcg cagcgaggac tgcctggtgc tgaacgtctg gacgcccgcc gtgaacgacg
     3481 ggcgcaagcg gccggtgatg gtctatttcc acggcggcgg ctattcgacc ggttcggcgg
     3541 ggtcgccgac gcaggacggg gcgcggctgg cggcgctgta cgacgtggtg gtggtcgcgc
     3601 ccaatcaccg gctggggctg ctgggctttc tgtggcttgg cgatctgctg ggcgcggatt
     3661 atgcgacctc gggcaatcag tcgatgttcg acatgatcgc gtcgctgcaa tgggtgcgcg
     3721 acaatatcgc ggccttcggc ggcgatcccg gcaatgtgac catcttcggc gaatcgggcg
     3781 gcggggccaa ggtcggcacg ctgatgggca tgccggcggc gaaggggctg ttccacaagg
     3841 cgggcatcca gagcggcgcg cagttgcggc gcatgacgcg cgccacggcg gcggagacgg
     3901 cgcaaaggct gatgcgcgcg ctcgacctca gcgacgcgcg ccgcctgcat gaggttccgg
     3961 tcgagacgct gctgaaactg caatgggaca gcgaggcggc gcctcaggcc gtcaagccgg
     4021 agatgacggg gatttcgttc tcggaatcga ccctgcccgg acattacggg ccggtggtcg
     4081 acggctgggc cttgcccgcc gatccgttcg acccggcggc gagcgatctg tgcgccgaca
     4141 taccgctgct gatcgccagc aacaagaccg aggcggcctt cttcttcatg aacgatccgg
     4201 cggcgttcgc gctggacgcg gcggggctga cgcggctgtt gcgcgacaat ctggggcggt
     4261 acgccgatac ggtcctgcaa acctatcgcg cgctgatgcc gaaggcgacg ccgtcggagc
     4321 tgttcctggc catcgccacg gcgcggacca tggggatcga gacgatcgtg ctggccgacc
     4381 gcaaggcggc gcaggccgcg ccggtgtacc gctatcgctg ggattacgcc tcgaacctgc
     4441 cggtggccgg gacatccgcg accctcggcg cgggccatgc gaccgacatc ggcccgacct
     4501 tcgccaattt cgacgaaaag gggctgcacg gcaatgggcc gggcgtgaag gcggcgtcgc
     4561 tgaacctcag cgcgatctgg gccggtttcg cccataccgg cgtggcgcgc gcgcccggcg
     4621 tggcggactg gccgcgctat acgccgcaga cgcgcgccac ggtgctggtc gatgaaaaat
     4681 gcgttctggc cgacgacccg gacgcggcgg cgcgcgagat gtggatgaag atcgatccgc
     4741 gctggggcta gggtccgtac tcaataaatg atggcgagtg agttaggttt gtgattcaa
//
LOCUS       DASVLL010000312         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_19576, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000312 DASVLL010000000
VERSION     DASVLL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19576"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VF402_05225"
     CDS             <1..622
                     /locus_tag="VF402_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799729.1"
                     /translation="SYKELFNSQGQSYQGNQTDQKNYTYDPSGLGGRNSDWIVDSSFV
                     LTKGLNGYERLRRDSDSFKPVQGEFGLSAITSKTQATLRYIFNDVLTEAQIQTLYRYS
                     TVLPSQNNLTTFGDNYRDFQLYAQHFFTKNWGVSARLDRDLVNNTWRRSTLSLIYKND
                     CIWYELVYQQNDSLLANYNHKPQSTILFRLNFATLGTSGHKFSDVR"
     gene            660..2057
                     /locus_tag="VF402_05230"
     CDS             660..2057
                     /locus_tag="VF402_05230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7799730.1"
                     /translation="MRFTTISAGKSLSRLALSRLALRHGAGAAAFALLIAAGVASAQD
                     VSAPPPLGAPNSGGAAFDSQAPLSPATQAPQLPQLGEGVLVSVNNDMITSYDLKQRML
                     LLIVTSGVQVTQDNYAAFQQQALNSLIDEKLEMQEMNHWKLKVTDKEVDNELKRMATQ
                     SNMSKDQLLSELKRVGVEADTLRQQIRAGIGWQELVGGRYQSNAAVGSAQVDAMMDKV
                     IADGQKPQYLVSEIFIDPQAAGSMQAAQQGAQQLYDQLQKKVAPFQAVARQFSNAPSA
                     ANGGDEGWMVSGNIDPAIETALKQLQPGEMSPPVMTKDGAYILLLRRKTDGDADMLFH
                     LKQAAAPLSDANAAAALTAFRDKVQSCDAFDDQNGKAAGGVQLTDLGEVQLSTLTPEY
                     SDALHPLKEGQVTAPLRNSQNTNILYVCSRQLAGENALSRSQLEDNLVNQRLAMLGKR
                     YLRELRASATIEHLQ"
     gene            2074..>2390
                     /locus_tag="VF402_05235"
     CDS             2074..>2390
                     /locus_tag="VF402_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase"
                     /protein_id="HEX7799731.1"
                     /translation="MTAPDTPLVISLGDPCGVGPEIVAKAWRALRDQPRLAFAVLGDA
                     DLLSRYAPVARCAASADAASAFADALPVLDRPLTAAVAPGRPDAAHAPHIIGWIREGA
                     GLCR"
BASE COUNT          481 a          803 c          693 g          413 t
ORIGIN      
        1 gtcctacaaa gagcttttca attcacaggg ccaatcttat cagggcaacc aaacggatca
       61 aaaaaattac acctacgatc cttctggtct tggcggccgc aattccgatt ggatcgtcga
      121 tagctcgttt gtacttacca aagggctgaa cggttacgag cgtcttcgtc gcgactcaga
      181 cagtttcaag ccggttcaag gtgaattcgg tctgagcgcg atcacctcga agacgcaggc
      241 cactctgcgc tacatcttca atgacgtgct taccgaagcg caaatccaga cgctctatcg
      301 atattcgacg gttcttccat cacaaaataa tcttacgact ttcggcgaca actaccgcga
      361 ctttcagttg tacgcccagc acttcttcac gaaaaactgg ggcgtgtcgg cgcgtctcga
      421 ccgcgatctg gtcaacaaca cctggcgtcg ttcgacgctt agcctgatct acaagaacga
      481 ttgcatatgg tatgaactgg tttatcagca gaacgacagc ctgctggcca actacaacca
      541 taaaccgcaa tcgaccatct tattccgact taacttcgcc acattgggaa cgtctggtca
      601 caagttctcc gacgtgcgct aatcccaata tgacgccaac aagctgacga atgtccctca
      661 tgcgattcac gactatttcc gccggtaagt ccctatctag actggccctg tccagactgg
      721 cgctgcgcca cggcgcgggc gcggcggcgt tcgccttgct catcgccgcc ggcgtcgctt
      781 cggcgcagga cgtctccgcg ccgccgccgc tcggcgcgcc gaacagcggc ggcgccgcct
      841 tcgactccca ggccccgctg tcgcccgcca cgcaggcgcc gcaactgccg caactgggcg
      901 aaggcgtgct ggtgtcggtc aataacgaca tgatcacctc ctacgacctg aagcagcgca
      961 tgctgctgct gatcgtcacc tccggcgttc aggtcacgca ggacaattac gccgccttcc
     1021 agcagcaggc cctgaacagc ctgatcgacg aaaagctgga aatgcaggag atgaaccact
     1081 ggaagctcaa ggtcaccgac aaggaagtcg ataacgagct gaagcggatg gccacgcaaa
     1141 gcaatatgag caaggaccag ctattgtccg aactcaagcg cgtcggcgtc gaggccgaca
     1201 cgctgcgtca gcagatccgc gccggcatcg gctggcagga actggtcggc gggcgttatc
     1261 aatccaacgc cgccgtcggc tcggcgcagg tcgacgccat gatggacaag gtgatcgccg
     1321 acggccagaa accgcaatat ctggtgtcgg aaatcttcat cgacccgcag gccgccggtt
     1381 cgatgcaggc cgcgcagcaa ggcgcgcagc agctttacga ccagttgcag aagaaggtcg
     1441 ccccgttcca ggccgtcgcg cgccagttct ccaacgcgcc gtccgccgcc aatggcggcg
     1501 acgaaggctg gatggtgtcc ggcaatatcg atcccgcgat cgaaaccgcc ctgaaacagt
     1561 tgcagcccgg cgagatgagt ccgccggtca tgaccaagga cggcgcctat atcctgctgc
     1621 tgcggcgcaa gaccgacggc gacgccgaca tgctgttcca cctcaagcag gccgccgcgc
     1681 cgctgtccga cgcgaacgcc gccgccgcgc tgaccgcctt ccgcgacaag gtccagtcct
     1741 gcgacgcctt cgacgaccag aacggcaagg cggcgggcgg cgtgcagctt accgatctgg
     1801 gcgaggtgca actgagcacg ctgacgccgg aatattccga cgccctgcat ccgctgaagg
     1861 aggggcaggt caccgccccg ctgcgcaaca gccagaacac caacatcctt tacgtatgca
     1921 gccgccagtt ggccggtgag aacgccctgt cgcgcagcca gctggaggac aacctcgtca
     1981 accagcgtct ggccatgctg ggcaagcgct atttgcgcga actgcgcgcg tcggccacga
     2041 tcgaacacct ccagtaagct gcggaggctc cccatgacgg cgcccgatac gccgctggtc
     2101 atcagtctgg gcgatccctg cggcgtcggt cccgagatcg tcgcgaaggc gtggcgcgcc
     2161 ttgcgggacc agcctcgcct cgccttcgcc gtgctgggcg acgccgacct gctcagccgg
     2221 tatgcgcccg tcgcgcgctg cgcggccagc gcggacgccg cttcggcctt tgccgacgcc
     2281 ctgccggttc tggaccgccc gctgacggcg gcggtcgcgc cgggcaggcc cgacgccgcc
     2341 catgcgccgc atatcatcgg ctggattcgc gaaggcgccg gtctgtgccg
//
LOCUS       DASVLL010000313         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_127470,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000313 DASVLL010000000
VERSION     DASVLL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127470"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VF402_05240"
     CDS             complement(<1..229)
                     /locus_tag="VF402_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEX7799732.1"
                     /translation="MPLTARLVADRDFVIAPLDRRVFGTFVEHMGRCVYGGIYEPGHA
                     TADANGFRQDVMALTRELGVTIVRYPGGNFLS"
     gene            complement(242..1555)
                     /locus_tag="VF402_05245"
     CDS             complement(242..1555)
                     /locus_tag="VF402_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7799733.1"
                     /translation="MKKLLVLALAVLALAGCAPKDSRTHITLQRFFGECRANVPKGKP
                     PLRNPDGECEVITRLLDQFRQQNPDIDLSVNIVAWPGYDQLSAEYAAGDPPDIAIMHQ
                     SAMPDYQKRGLIAPLDADFRQAGIDPADFTPAARQGVTIDGHVYGMPFDNWMQLWHIN
                     TALFARAGLMKDGQPVLPHSPDELIQQARQFKARTGLPYLIQATANEQASYTRNLYTY
                     LMDQHAVFFTDPKHIRLDTPEARRVVDLFRRMYAEGLMTHDQDYPAATQAFLNGQGGV
                     YQVGTWMIGTFTSESETPGHPLYRAYTVKPYPMLYGSAPTAYVDGHAWVMPTRPRTPA
                     QKEAVLRLMKFLQQHDFDWSRTGHLPSQIQVAQSAQYLALPHRRDIIGLATVGRQLPH
                     DIQRQFAISDIIGDEMASAVTGHKPTEQALQDAQRRVNDLLYNLI"
BASE COUNT          269 a          491 c          603 g          293 t
ORIGIN      
        1 cggacaggaa gttgccgccc ggatagcgca cgatggtcac gcccagctcg cgggtcagcg
       61 ccatcacgtc ctgccggaag ccgttggcgt cggcggtggc gtggccgggc tcgtaaatgc
      121 cgccatagac gcagcggccc atatgctcga cgaaggttcc gaacacgcgg cggtccagcg
      181 gagcgatgac gaagtcgcgg tcggcgacga ggcgggccgt aaggggcatg atgatcaaac
      241 ctcagatgag attgtagaga aggtcgttga cgcggcgctg ggcgtcctgc aacgcctgct
      301 ccgtcggttt gtggccggtg acggccgagg ccatctcatc gccgatgatg tcggagatgg
      361 cgaactggcg ctggatatcg tgcggcaatt gccggccgac cgtggcgagg ccgataatgt
      421 ccctgcggtg cggcagggcc aggtactgcg cgctttgcgc gacctgaatc tggctgggca
      481 ggtggccggt gcgcgaccag tcgaaatcgt gctgttgcag gaacttcatc agccgcagca
      541 cggcctcctt ctgcgcgggc gtgcgcgggc gcgtcggcat gacccaggcg tggccgtcga
      601 cataggcggt cggcgccgag ccgtacagca tcggataggg cttgaccgta taggcgcgat
      661 agagcggatg gccgggcgtt tcgctctcgc tggtgaaggt tccgatcatc caggtgccga
      721 cctgatagac gccgccctga ccgttgagga aggcctgagt cgccgccgga tagtcctgat
      781 cgtgcgtcat cagcccctcg gcgtacatcc ggcggaacag gtcgacgacg cggcgcgctt
      841 cgggggtgtc gagcctgatg tgcttcgggt cggtgaaaaa gaccgcgtgc tggtccatca
      901 ggtaggtgta gaggttgcgc gtataggagg cctgttcgtt ggccgtcgcc tgaatcaggt
      961 acggcaggcc ggtgcgcgcc ttgaactggc gcgcctgctg tatcagctcg tccgggctgt
     1021 gcggcaggac cggctggccg tccttcatca gcccggccct ggcgaacagc gccgtattga
     1081 tgtgccagag ctgcatccag ttgtcgaacg gcatgccgta gacatggccg tcgatggtca
     1141 cgccctgccg cgccgccggg gtgaagtccg ccgggtcgat gccggcctgc cggaaatcgg
     1201 cgtccagcgg cgcgatcagg ccgcgcttct ggtaatcggg catggccgac tggtgcatga
     1261 tggcgatgtc gggcggatcg cccgccgcgt actccgcgga aagctggtcg tagccgggcc
     1321 aggcgacgat gttgacgctg aggtcgatgt cgggattctg ctgccggaac tggtccagca
     1381 ggcgggtgat gacctcgcat tcgccgtccg ggttgcgcag cggcggcttg cccttgggca
     1441 cattggcgcg gcattcgccg aagaagcgtt gcagggtgat gtgcgtgcgc gaatccttcg
     1501 gcgcgcagcc cgccagagcg agaacggcga gggcgaggac gagcagcttt ttcatcgcac
     1561 ggccgctccc gacaccgcct tgacgatgta cttctggaag atgacataga cgatcaggat
     1621 cggcacggcg gcgaacacgg cctgcgtcat caggta
//
LOCUS       DASVLL010000314         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_289013,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000314 DASVLL010000000
VERSION     DASVLL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289013"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF402_05250"
     CDS             complement(<1..234)
                     /locus_tag="VF402_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799734.1"
                     /translation="MSDRAERIRVNEAALKARLEALRVFSEGVAARLCELEMPETYLE
                     AARGARALDQADRLLLRMPGLDLDFNGPLPGADG"
     gene            378..1889
                     /gene="gatB"
                     /locus_tag="VF402_05255"
     CDS             378..1889
                     /gene="gatB"
                     /locus_tag="VF402_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7799735.1"
                     /translation="MTNALDIRAAEASQHIMGRTGPWELVIGLEVHAQVASHTKLFSG
                     ARVGFGAGPNEQVSLVDAAMPGMLPVLNRFCVEQAVKTGLGLRAKINRFSRFDRKNYF
                     YPDLPQGYQISQLFHPIVGEGEVLVEKDDGTAITVRIERLHLEQDAGKSIHDLDPNAT
                     YVDLNRAGTALMEIVSKPDIRSSDEAVAYFKKLRTILVYLGTCDGDMEKGNMRADCNV
                     SVRRPGEGFGTRCEIKNVNSFRFMQQAIEYEARRQIEILEDGGAIDQETRLFDANKGE
                     TRSMRSKEEAHDYRYFPDPDLLPVELEEAWIEAIRQHLVELPDDKKARFIRQYGLSAY
                     DAGVLISDQARADYFEAAVLKLKEFGGRDAKLVANWVTNELLARLARDGQEIADSFIA
                     PAHIAGLVGLIENGTISSRIAKTVFEHMWDEKGILSPAEIVEKHGLKQVTDTGAIEKA
                     IDEIIAANPDKAEAVKDKPQALGWFVGQVMKATGGKANPAAVNDLLKAKLGVA"
     gene            complement(1955..2182)
                     /locus_tag="VF402_05260"
     CDS             complement(1955..2182)
                     /locus_tag="VF402_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799736.1"
                     /translation="MTRTISLTALAVLLAALAACSAPKKDGDAMSMSASSSDAMAPMA
                     GGDSMAMSSSPDAMAPAMSSGAMSSGGAMSH"
     gene            2334..3260
                     /locus_tag="VF402_05265"
     CDS             2334..3260
                     /locus_tag="VF402_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7799737.1"
                     /translation="MSDVALRLAGVGKSFDGFEAVRDLSFEVPRGAICGFLGPNGAGK
                     TTSVRMMLGLTAPTRGEIEVLGAPVGRWLDRIGFMPEERGLYRKMTALDVIVFLAGLK
                     GVKAGAARERGRRMLEAQGLGAFAHTQIRSLSKGMSQKVQLISCLVHEPELVILDEPF
                     SGLDPVNQEGLEAMIRDLAQGGATVLFSTHVMQHAERLCEKVVLMAKGRKRFEGTLKA
                     ARSAAPRYLDLEGALTRDDCLALPGIAAAEDMGTGEGGRLWRCRLTGEASAHEALKAA
                     FLKGLDVRRFEAREPSLHDAFIVLTSEADAHV"
     gene            3253..>4020
                     /locus_tag="VF402_05270"
     CDS             3253..>4020
                     /locus_tag="VF402_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7799738.1"
                     /translation="MSKTLLIAKREYLAFVRTMGFWLSLVTLPLLITAVIAVPLLLRQ
                     SAPVTVLSAAVLDLSAEHVGGDLEALIRAQAAPDDGLKGLAAKVAHADQVRLAPLPPG
                     LSAAMTPADAEAKIPAILNAPHAKVSNIIVVYDQGDVLQFHIWSTPKQKGKLADRLQG
                     DLNGLQYDKLAREHGVDAKLARDMREARAGVLSLTSVTGGGGDGFSQTLRENAPRVAG
                     MLTGYLAWMSIFSSSMILLSGVIEEKSSKVLEVVLASS"
BASE COUNT          704 a         1172 c         1472 g          672 t
ORIGIN      
        1 cccgtccgcc cccggcaacg gcccattaaa atccagatcg agccccggca tccgcaacaa
       61 caaacgatcg gcctgatcga gcgcacgcgc cccgcgcgcg gcttcgagat acgtctccgg
      121 catctccagc tcacacaacc gcgccgccac gccctcagaa aacacgcgca aagcctcaag
      181 ccgcgccttc aacgcagcct cattgaccct gatccgctcc gcccggtccg acatgcggaa
      241 cagaatagcg gcggttcgag ggggtgggga ttactgtacg gtcaaggagc ccttaatcga
      301 gtcatcgatg ctttggagga ttccaattga cggaatttcc tgatgcaatt tgaacgctcc
      361 cccgctatat cccccctatg acaaacgctc tcgatattcg cgccgctgag gcttcgcaac
      421 acattatggg ccggacgggg ccttgggaac tggtgatcgg gctggaggtg catgcgcagg
      481 tggcgtcgca caccaagctg ttttccggcg cgcgggtcgg gttcggggcg gggccgaacg
      541 agcaggtgtc gctggtggac gcggccatgc cgggcatgtt gccggtgctg aaccggttct
      601 gcgtcgagca ggcggtcaag acggggctgg ggctgcgggc gaagatcaac cggttctcgc
      661 gcttcgaccg caagaactat ttctatcccg acctgccgca gggctatcag atcagccagt
      721 tgttccaccc catcgtgggc gagggcgagg tgctggtcga aaaggatgac ggcacggcga
      781 tcaccgtgcg catcgagcgg ctgcatctgg agcaggatgc gggcaaatcg atccacgacc
      841 tcgatcccaa tgcgacctat gtcgacctga accgcgcggg cacggcgctg atggagatcg
      901 tgtccaagcc ggatatccgc tcttccgacg aggcggtggc ttacttcaag aagctgcgca
      961 ccatccttgt gtatctcggc acctgcgacg gcgacatgga aaagggcaat atgcgcgccg
     1021 attgcaacgt gtcggtgcgc cgtccgggcg aggggttcgg cacgcgctgc gagatcaaga
     1081 acgtcaacag cttccgcttc atgcagcagg ccatcgagta cgaggcgcgg cgccagatcg
     1141 aaatcctgga ggacggcggc gccatcgatc aggagacgcg cctgttcgac gccaacaaag
     1201 gcgagacgcg ctcgatgcgc tccaaggagg aggcgcatga ttatcgctac ttccccgatc
     1261 ccgacctgtt gccggtggag ttggaggaag cgtggatcga ggcgattcgc cagcatctgg
     1321 tcgaactgcc cgacgacaag aaggcgcgct tcatccgcca gtacgggctg agcgcctacg
     1381 acgccggggt gctgatctcg gatcaggcgc gggcggacta tttcgaggcg gcggtcttga
     1441 agcttaaaga atttgggggc cgcgacgcca agctggtcgc caactgggtg acgaacgaac
     1501 tgctggcgcg gctggccagg gacgggcagg agatcgccga ttcgttcatc gcgccggcgc
     1561 atatcgccgg gctggtcggg ctgatcgaga acgggaccat ctcgtccagg atcgccaaga
     1621 cggtgttcga gcatatgtgg gacgagaagg gcatcctgag cccggccgaa atcgtcgaga
     1681 agcacggcct gaaacaggtg accgacaccg gcgccatcga gaaggccatc gacgaaatca
     1741 tcgccgccaa cccggacaag gccgaggcgg tcaaggacaa gccgcaggcg ctgggctggt
     1801 tcgtcggaca ggtgatgaag gcgaccggcg gcaaggccaa tccggcggcg gtcaacgatc
     1861 tgctgaaggc gaagctgggg gtggcgtagt ttcgcccccg ccctgtcgcg gcgcaagacg
     1921 agaaagcccc cggttcgcgc cgggggcttt cgttttagtg gctcatggcg ccgccggagg
     1981 acatggcgcc ggaggacatg gcgggcgcca tcgcatccgg cgacgaactc atggccatgc
     2041 tgtcgccgcc ggccatcggg gccatcgcgt cggacgacga ggcgctcatg gacatggcgt
     2101 cgccgtcctt tttcggggcg gagcaggcgg ccagagcggc gagcaggacg gcgagggcgg
     2161 tcaggctgat ggtgcgggtc atgtggagtt tcctcagata gggtgggaca gtcgcgctgt
     2221 cgcaagggga tacgtcgcgg cggcgcgaac ggttacaaag gcgggttttc cttttggggg
     2281 gctttccttt ggcggcgcgc ggccttaggc tcggcgcgga acggggagtc tccatgagcg
     2341 atgtggcgtt gcggctggcg ggagtcggca agagcttcga cgggttcgag gcggtgcggg
     2401 acttgagttt cgaggttccg cgcggcgcga tctgcggatt tctggggccg aacggcgcgg
     2461 gcaagacgac cagcgtgcgc atgatgctgg ggctgacggc gccgacgcgc ggcgagatcg
     2521 aggtgctggg cgcgcctgtc gggcgctggc tggaccggat cggcttcatg ccggaagagc
     2581 gcgggcttta tcgcaagatg accgcgctgg acgtgatcgt gtttctggcg ggcctgaaag
     2641 gcgtcaaggc gggcgcggcg cgcgagcgcg ggcggcggat gctggaggcg caaggtctgg
     2701 gcgcgttcgc ccatacgcag atccgctccc tgagcaaggg catgtcgcag aaggtgcaac
     2761 tgatttcctg cctggtgcat gagccggagc tggtgattct cgatgagccg ttttccgggc
     2821 tggacccggt caatcaggag gggctggagg cgatgatccg cgatctggct cagggcgggg
     2881 cgacggtgct gttctcgacc catgtgatgc agcacgccga gcgcctgtgc gagaaggtgg
     2941 tgctgatggc caaggggcgc aagcgcttcg agggcacgct gaaggcggcg cggtcggcgg
     3001 cgccgcgcta tctcgatctg gagggggcgc tgacgcggga cgattgcctg gccctgccgg
     3061 ggatcgcggc ggcggaggat atgggaacgg gcgagggcgg tcgcctttgg cgctgccggt
     3121 tgacgggcga ggccagcgcg catgaggcgc tcaaggcggc ctttctcaaa gggctggacg
     3181 tgcgccggtt cgaggcgcgc gagccgagcc tgcacgacgc ctttatcgtg ctgacctcgg
     3241 aggcggacgc gcatgtctaa gactctgctg atcgcgaaac gcgaatatct ggcctttgtc
     3301 aggacgatgg ggttctggct gtcgctggtg accctgcctt tgctgatcac ggcggtgatc
     3361 gcggtgccgc tgctgctgcg ccagagcgcg ccggtgacgg tgctgtcggc ggcggtgctg
     3421 gacctgagcg ccgagcatgt cggcggcgat ctcgaggcgc tgatccgggc gcaggcggcg
     3481 ccggacgacg ggctgaaagg gctggccgcg aaggtggcgc acgccgatca ggtgcggctg
     3541 gcgcccctgc cgccgggtct gagcgcggcc atgacgcccg ccgatgccga ggcgaagata
     3601 ccggccatcc tgaacgcgcc acacgcgaaa gtcagcaata tcatcgtcgt ctatgaccag
     3661 ggcgacgtgt tgcagttcca tatctggtcg acgcccaagc agaagggcaa gctggccgac
     3721 cggctgcaag gggatctgaa cgggctgcaa tatgacaagc tggcgcgcga gcatggcgtc
     3781 gatgcgaaac tggcccgcga catgcgcgag gcgcgcgcgg gcgtcctcag cctgacgtcc
     3841 gtgaccgggg gcgggggcga cggtttcagt cagaccttac gcgaaaacgc gccgcgcgtg
     3901 gcggggatgc tgaccggcta tctggcctgg atgagcatct tttcgtcgtc gatgatcctg
     3961 ttgtcggggg tgatcgagga gaagtcgagc aaggtgctgg aggtggtgct ggcgtcgagt
//
LOCUS       DASVLL010000315         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_19808, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000315 DASVLL010000000
VERSION     DASVLL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19808"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /gene="trxA"
                     /locus_tag="VF402_05275"
     CDS             <1..821
                     /gene="trxA"
                     /locus_tag="VF402_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7799739.1"
                     /translation="FMNDVIEASMTTPVIVDFWATWCGPCRQLGPALEKVVGEQKGKV
                     KLVKIDVDQNQAIAAQLRIQSIPTVYAFVGGRPVDGFMGAKSETELRAFIDKLGVGDA
                     GPSIEDTLALAAQSAADGDATSALEAYSFVLEQEPENPKAIAGIARVYLKAGQAEQAK
                     AFLEQAGDSKDPEILGLKAALELAEGAPTDIGGLRNRIAADARDFEAWYDLGRALAAQ
                     GETAEAIDALLEIVKYQPDHQAARDYLVKIFTAVGNDSPVVRAGRRRLSSMLFS"
     gene            831..1037
                     /locus_tag="VF402_05280"
     CDS             831..1037
                     /locus_tag="VF402_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX7799740.1"
                     /translation="MSEAVKPSAEIDPRLLEALVCPVTRRPLTYDRAAQELISPTAGL
                     AYPIRSGVPIMLVDQARKFEPQRA"
     gene            complement(1185..2429)
                     /locus_tag="VF402_05285"
     CDS             complement(1185..2429)
                     /locus_tag="VF402_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEX7799741.1"
                     /translation="MKTYDVVIVGAGMAGMTLALALHQGGLRVALVERQSFGDILDVA
                     YDGRASAIAYACFRQWRALGVGGALEAHACRMDEIMVTDGRLPGAATAKPLPFFLHFT
                     AGEISGRTGDEPLGYMVENRQTRKALYEAVTQRGVDLYCPASVRDSRETAAMAQIDLD
                     TGETLRAPLIVSAEGRRSTLRDKAGIHYINWDYDQSAVVCTVKMERPHNHVAYEHFLP
                     TGPFAILPLTDNRACIVWSEKHAKAKALMAVRPELFHDHLMQRFGDFLGAVEVVGQPF
                     LYPLGLGLAAEMHKGRLALLGDAAHGIHPIAGQGLNLGLKDVAALSEVLIEAARLGED
                     LGSSLVLERYAKWRRFDNVSTTAIMDGFVRLFSNDNPLVRAARDVGMGMFNRVPALRR
                     FVMEDAGAATGDLPKLLKGELV"
     gene            2591..>4425
                     /locus_tag="VF402_05290"
     CDS             2591..>4425
                     /locus_tag="VF402_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX7799742.1"
                     /translation="MASLTATLWTAFETHARNIWTSTPMARLRGAFTALAGCAGVLAL
                     ATYNPDDPSLNTASGGDVHNMLGHTGAIAADAAAQSLGLAAWPAAALLIYFGLQRVFH
                     PDPDQTRGRLRLQALWAVLFVLALAAAIAPLQTADDPVLAQSVGGFWGAGLHHLLAAL
                     FGFMRLPGAVVIASLIFAGLAVFGFNQTFSLKFDAYLRAAQATAGLLAEAMGRFTHRA
                     TSPEDEAPARPKRAKKVKTFVDDGEADGFVAPVRPRMTLDDDDDAPRPEPEIAPTVRF
                     APPTDEMDFGDDDGAPIDIQPMKPAPQAAPAKQPEFDFLRPGNFRLPELSILAKPQAR
                     SHAYDEDSLRQNARMLESVLAEFGVKGVIDQIRPGPVVTLYELAPAAGVKGARVVALA
                     DDIARNMSARSCRVSVVQGRNAIGIELPNHKRETVYLRDLLASKDYEATNQTLPMALG
                     ESIGGEPYIADLAKMPHLLIAGTTGSGKSVGVNAMILSILYRLDPEQCKFIMIDPKML
                     ELSVYDGIPHLLAPVVTDPKKAVVALKWTVKEMEDRYRRMSKVGVRNIANYNERARAT
                     AAEGKNFVRKVQTGFDETGQPVYEFDEMVPEPMPYLVVVVDEVADL"
BASE COUNT          778 a         1495 c         1432 g          720 t
ORIGIN      
        1 cctttatgaa cgatgtcatc gaggcgtcga tgaccacgcc ggtcatcgtc gatttctggg
       61 cgacctggtg cgggccgtgc cgtcagcttg gcccggcgct ggagaaggtg gttggtgagc
      121 aaaagggcaa ggtgaagctg gtcaagatcg acgtcgatca gaatcaggcc atcgccgcgc
      181 aactgcgcat ccagtcgatc ccgaccgtct atgcctttgt cggcggccgg ccggtggacg
      241 gctttatggg cgccaagtcc gaaaccgaac tgcgcgcctt tatcgacaag ctgggcgtcg
      301 gcgacgccgg gccgagcatc gaggacaccc tggccctggc cgcgcagtcc gcggccgacg
      361 gcgacgcgac ctcggcgctg gaagcttaca gtttcgtgct ggagcaggag ccggagaacc
      421 ccaaggccat agccgggatc gcgcgcgtct atctcaaggc tggtcaggcc gaacaggcca
      481 aggcgtttct ggagcaggcg ggggactcga aagacccgga gattctcggc ctgaaagccg
      541 cgctggagct ggccgaaggc gcgcccaccg acatcggcgg cttacgcaac cggatcgcgg
      601 cggacgcgcg cgatttcgag gcgtggtacg atctgggccg cgccttggcc gcgcaaggcg
      661 agacggcgga ggccatcgac gcgctgctgg aaatcgtcaa gtatcagcct gaccatcagg
      721 cggcgcgcga ctatctggtc aagatcttca ccgccgtcgg caacgactcg cccgtggtgc
      781 gcgccggtcg ccgccgcctg tcctcgatgc tgttttcgta aaggtccgcc atgtccgaag
      841 ccgtcaagcc gtccgccgaa atcgacccgc gtttgctgga ggcgctggtc tgtccggtta
      901 cgcgccgtcc gctgacctat gacagggcgg cgcaggagct gatcagcccg acggcggggc
      961 tggcctatcc gatccggtcg ggggtgccga tcatgctggt ggatcaggcg aggaagtttg
     1021 agccccaaag agcgtagtga aggggtagaa ggcaaggggt cattgacccc ttgaccccga
     1081 aactgtcctt taggaacaac aaaaaaccgg gcctcaagag gtccagcttt ttgttgtttt
     1141 caaagactaa tttccggggt caaggggcca atcggcccct tgccttaaac caactccccc
     1201 ttcaacagct tcggcagatc gccggtcgcc gcgcccgcat cctccatcac gaacctgcgc
     1261 aaggcgggca cgcggttgaa catccccatg ccgacgtcgc gcgccgcgcg caccagcggg
     1321 ttgtcgttgg agaacagccg cacgaagccg tccatgatcg ccgtggtcga gacattgtcg
     1381 aagcgccgcc acttcgcgta gcgctccagc accagagacg agccgaggtc ttcgcccaac
     1441 cgcgccgcct cgatcagcac ttccgacagc gccgccacgt ccttcaggcc gagatttaag
     1501 ccctgtcccg cgatcgggtg gatgccgtgc gccgcgtcgc ccagcagggc caggcgcccc
     1561 ttgtgcatct ccgccgccag acccaggccc agcggataga gaaacggctg gcccaccacc
     1621 tcgaccgcgc cgaggaagtc gccgaagcgc tgcatcaggt gatcgtggaa caactccggc
     1681 ctgaccgcca tcaaagcctt cgccttggcg tgcttttccg accagacgat gcaggcgcgg
     1741 ttatcggtca gcggcaggat ggcgaacggc ccggtcggca ggaaatgctc ataggcgacg
     1801 tggttgtgcg gccgctccat cttcacggtg cagaccacgg cgctctggtc gtaatcccag
     1861 ttgatgtaat ggatgcccgc cttgtcgcgc aaggtcgagc gccgcccctc cgccgagacg
     1921 atcagcggcg cgcgcagggt ctcgcccgta tcgaggtcga tctgcgccat cgccgccgtc
     1981 tcgcggctgt cccggaccga agccgggcaa tagaggtcga cgccgcgctg cgtcaccgcc
     2041 tcatagagcg ccttgcgcgt ctggcggttc tcgaccatgt agcccagcgg ttcgtcgccg
     2101 gttctgccgg aaatctcgcc cgccgtgaag tgcaggaaaa agggcagggg tttggccgtc
     2161 gccgcgccgg gcaggcgccc gtccgtgacc atgatctcgt ccatgcggca ggcgtgcgcc
     2221 tccagcgcgc cgccgacgcc cagcgcccgc cattgccgga aacaggcgta ggcgatggcc
     2281 gaggcgcgcc cgtcataggc gacgtcgagt atgtccccga aactctggcg ctcgaccagc
     2341 gcgacccgca gcccgccctg atgcagggcc agcgccagag tcatgccggc catgccggcg
     2401 ccgacgatca caacgtcata ggttttcatg cgcgtactcc tgcgccgcat cataaacgcc
     2461 gcccgtcaaa gcgcaatgcg gcaggcgcgc gcccggaaaa aagcagatat cgtaaacagg
     2521 ttttaaccat ttgccgggca tctaggggag gagatttcgc ccctgtttta aagagttacc
     2581 gcagaccgac atggccagcc tgaccgccac cctctggacc gctttcgaga cgcatgcgcg
     2641 caatatctgg acctcgaccc cgatggcgcg gctgcgcggc gccttcaccg cgctggccgg
     2701 atgcgcgggc gttctggcgc tggcgaccta taacccggac gaccccagcc tgaacacggc
     2761 gtccggcggc gacgtgcata atatgctggg ccataccggc gccatcgccg ccgacgccgc
     2821 cgcgcagtcg ctggggctgg cggcgtggcc cgccgcggcg ctgctgatct atttcggcct
     2881 gcaacgcgtc ttccatcccg acccggacca gacgcgaggc cggttacgtc ttcaggcttt
     2941 gtgggccgtg ctgttcgtgc tggccctggc cgccgccatc gcgcccttgc agaccgcgga
     3001 cgatccggtg ctggcgcaaa gcgtcggcgg cttctggggc gcggggctgc atcacctgct
     3061 ggcggcgctg ttcggtttca tgcgcctgcc cggcgcggtc gtgatcgcca gcctgatctt
     3121 cgccggtctg gccgttttcg gcttcaacca gaccttcagc ctgaaattcg acgcctatct
     3181 gcgcgccgct caggcgaccg ccggtctgct ggccgaggcg atgggccgtt tcacccaccg
     3241 cgccaccagc ccggaagacg aggcgcccgc gcgcccgaaa cgcgcgaaga aggtcaagac
     3301 ctttgtcgac gacggcgagg ccgacggctt tgtcgcgccg gtccgtccgc gcatgacgct
     3361 ggacgacgat gacgacgcgc cgcgcccgga gccggaaatc gcgccgacgg tccggttcgc
     3421 gccgccgacg gacgagatgg atttcggcga cgacgacggc gcgcccatcg acatccagcc
     3481 gatgaaaccg gcgccgcagg ccgctccggc caagcagccg gaattcgact tcctgcgccc
     3541 cggcaatttc cgcctgccgg agctgagcat cctggccaag ccgcaggcgc gcagccacgc
     3601 ctatgacgaa gattccttgc gccagaacgc ccggatgctg gaaagcgtgc tggccgaatt
     3661 cggcgtcaag ggcgtgatcg atcaaattcg ccccggtccg gtggtgacgc tgtacgaact
     3721 ggccccggcg gcgggcgtga agggcgcgcg cgtcgtggcg ctggccgacg acatcgcgcg
     3781 caacatgtcg gcgcgctcgt gccgcgtctc ggtggtgcag ggccgcaacg ccatcggcat
     3841 cgaactgccc aaccacaagc gcgaaacggt ctatttgcgc gaccttttgg cctcgaagga
     3901 ttacgaggcg accaaccaga ccctgccgat ggcgctgggc gaaagcatcg gcggcgagcc
     3961 ttacatcgcc gacctcgcca agatgccgca cctcctgatc gcggggacca ccggttcggg
     4021 caagtcggtc ggggtcaacg ccatgatcct gtccatcctc taccggctcg atcccgaaca
     4081 gtgcaaattc atcatgatcg accccaagat gctggagctg tcggtctatg acggcattcc
     4141 gcacctgctg gcgccggtgg tgaccgatcc gaaaaaggcc gtggtcgcgc tgaaatggac
     4201 ggtcaaggag atggaggacc gttatcgccg catgtccaag gtcggcgtgc gcaacatcgc
     4261 caactataac gaacgcgcca gagccaccgc cgccgaaggc aagaacttcg tgcgcaaggt
     4321 gcagaccggc ttcgacgaga cgggccagcc cgtctacgag ttcgacgaaa tggtccccga
     4381 acccatgccc tatctggtcg tggtggtcga tgaggtcgcc gacct
//
LOCUS       DASVLL010000316         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_504754,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000316 DASVLL010000000
VERSION     DASVLL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504754"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VF402_05295"
     CDS             complement(<1..421)
                     /locus_tag="VF402_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEX7799743.1"
                     /translation="MTAVPHKRFEFGTVFGDAGHIVSQPQREKKHYTPEEVEAIRAAA
                     YAEGEASASARAQMAQAAAVQQLADAAAAGLNGLTQAVHAHKEQAVRLALACAQKIAA
                     EALEQFPHAALQAALDALAQEIQPAARLLLNAREPDDA"
     gene            complement(551..1573)
                     /gene="fliG"
                     /locus_tag="VF402_05300"
     CDS             complement(551..1573)
                     /gene="fliG"
                     /locus_tag="VF402_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX7799744.1"
                     /translation="MSRKNVIEDPKRLSGVEKAAVVLLALGEDHVALWQALDEEEIKE
                     ISQAMSSLGTVSSAAVEELLVEFVSGMSSSGTIMGSFEQTQRLLQSFLPADKVEALME
                     EIRGPAGRTMWDKLGNVNEAVLANYLKNEYPQTVAVVLSKIRPDHASRVLSALPEDFA
                     LECVQRMLRMEPVQREILDKIEQTLRVEFMSNLARTSKRDSHELMAEIFNNFDRQTES
                     RFVAALEERNRESAERIRALMFVFEDLGKLDPGGVQTLLRAVDKEQLALALKGASDSL
                     RDMFMSNMSERAAKIMRDDMEAMGPVRLKDVDAAQMNMVQIAKDLAAKGEIMLAGQGG
                     EDELIY"
     gene            complement(1581..>1872)
                     /locus_tag="VF402_05305"
     CDS             complement(1581..>1872)
                     /locus_tag="VF402_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HEX7799745.1"
                     /translation="QPSLIAVAGPSGQMQMVPAGVAVAGGAPVLAEAGGGFPAISGGG
                     GQLAPVGAAEERIDIARIEGQVRASSVKKVSEFVERHPEESVSILRSWLHDG"
BASE COUNT          307 a          686 c          568 g          311 t
ORIGIN      
        1 aggcgtcgtc cggttcgcgc gcgttgagca gcaggcgcgc ggcgggctga atctcctgcg
       61 ccagcgcgtc gagcgcggcc tgcaacgccg cgtgcggaaa ctgctccagc gcctcggcgg
      121 cgatcttttg cgcgcaggcc aaagccagcc gcaccgcctg ctccttgtgc gcatgcaccg
      181 cctgcgtcag cccgttcagc cccgccgccg ccgcgtcggc cagttgctgc acggccgccg
      241 cctgcgccat ctgcgcgcgc gccgacgccg acgcctcgcc ctcggcatag gcggccgcgc
      301 ggatcgcctc gacctcttcg ggcgtatagt gcttcttctc gcgctgcggc tgcgacacga
      361 tatgccccgc gtcgccgaac accgtgccga actcgaaacg cttatgcgga acggctgtca
      421 tgacagagcc atccctgtac aaactacgcc gatacaagct cccggagcga agcgactagc
      481 gccgttgagg cgcgcagcct gcgtggcgcc aggcgagcga aagcgagctg aacgcaaagc
      541 aaaaaactca ctaataaata agctcatcct cgcctccttg cccggccagc atgatctcgc
      601 ccttggcggc gagatccttg gcgatctgca ccatgttcat ctgcgcggcg tcgacgtcct
      661 tgaggcgcac cggtcccatc gcctccatgt cgtcgcgcat gatcttggcg gcgcgttcgg
      721 acatgttgga catgaacatg tcgcgcaggg agtccgacgc gcccttgagc gccagcgcca
      781 gttgctcctt gtcgaccgcg cgcaaaaggg tctgcacccc gccggggtcc agcttgccca
      841 gatcctcgaa cacgaacatc agcgcgcgga tgcgctcggc ggattcgcgg ttgcgctcct
      901 ccagcgcggc gacgaagcgg ctctcggtct ggcggtcgaa attgttgaag atttccgcca
      961 tcagctcatg cgaatcgcgc ttggacgtgc gcgccaggtt ggacatgaac tccacgcgca
     1021 gggtctgctc gatcttgtcg aggatttcgc gctgcaccgg ctccatgcgc agcatgcgct
     1081 gcacacattc cagcgcgaag tcctccggca gcgccgacag cacgcgcgag gcgtggtccg
     1141 ggcggatctt ggacagcacc accgccacgg tctgcggata ttcgttcttc agatagttgg
     1201 ccagcaccgc ctcgttgacg ttgcccagct tgtcccacat ggtccgtccg gcggggccgc
     1261 ggatttcctc catcagcgcc tcgaccttgt cggcgggcag gaaggattgc aaaaggcgct
     1321 gggtctgctc gaacgacccc atgatggtgc cggaggacga catgcccgac acgaattcga
     1381 ccagcaactc ctcgaccgcc gccgacgaca cggtgccgag gctggacatg gcctgcgaga
     1441 tctccttgat ctcctcctcg tccagcgcct gccacagggc gacgtgatcc tcgcccagcg
     1501 ccagcagaac gaccgccgcc ttttcgacgc cggacaaccg cttcggatcc tcgatgacgt
     1561 tcttgcgcga catttacacc ctacccgtca tgcagccagg aacgcaggat cgacaccgat
     1621 tcctccgggt ggcgctcgac gaattccgac accttcttga ccgacgaggc gcgcacctgg
     1681 ccctcgatgc gcgcgatgtc gatgcgctcc tccgccgccc cgaccggcgc cagttgcccg
     1741 ccgccgcccg aaatcgccgg gaagccgccg cccgcctcgg ccagaaccgg cgccccgccc
     1801 gccacggcga cgccggcggg gaccatctgc atctgccccg acggaccggc caccgcgatc
     1861 aggctcggct gg
//
LOCUS       DASVLL010000317         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_73513, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000317 DASVLL010000000
VERSION     DASVLL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73513"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="narH"
                     /locus_tag="VF402_05310"
     CDS             <1..727
                     /gene="narH"
                     /locus_tag="VF402_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521248.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX7799746.1"
                     /translation="RYLGVLLYDADRIEAAASVDDKDLYQAQLDVFLDPHDPDVIRQA
                     RADGIAEAWLEAARNSPVYKMAMDWKIAFPLHPEYRTLPMVWYVPPLSPIQSAASAGA
                     LEMDGEMPDVHSLRIPARYLANLLTAGVEAPVIAALQRMMTMRMFMRARTIEGRIDAP
                     LAEKVGLSVAQIEDMYRIMAIANYEDRFVIPTTHREAAEDAYELRGNCGFTFGNGCSG
                     GQTEFGLFGKIKKPAAAPLAKEA"
     gene            730..1437
                     /gene="narJ"
                     /locus_tag="VF402_05315"
     CDS             730..1437
                     /gene="narJ"
                     /locus_tag="VF402_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX7799747.1"
                     /translation="MALTYRALAQLLTYPTADMQSLAADIARVLSGEGLLRADLIDGV
                     SALARHLAAADIYDVQEAYIDLFDRSRSLSLNLYEHVHGESRERGQAMAALVELYASR
                     GLELDARELPDYLPVFLEFLSTLSQPEAASLLGEAVHVLEALSQRLGKRESPYRAVFD
                     ALVALAGVRAEAQVLAALLAEPEDNPDDLDALDRQWAEQAVTFGPGEAGCPKAEALVA
                     AMQADEPAPRRAAGGAD"
     gene            1437..2144
                     /gene="narI"
                     /locus_tag="VF402_05320"
     CDS             1437..2144
                     /gene="narI"
                     /locus_tag="VF402_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX7799748.1"
                     /translation="METINSLLFGVYPYIALSVLVIGSVLRFDREQYTWRSGSSQLLR
                     RKQLVAGSVLFHLGVLVILGGHMVGLLTPIAVFEYLGIPHGAKQVLAMVAGGVAGVFA
                     LAGALLLLHRRLFDARVRHNSTFGDTAILIVLTVQLCLGLATIPLSAQHLDGSEMVKF
                     MNWAQGVFTFNRQAAGYVADVPWVFKAHLILGMTILLVFPFTRLVHMLSAPVWYLNRR
                     GWQLVRTRRDIAALRRK"
     gene            2148..3227
                     /locus_tag="VF402_05325"
     CDS             2148..3227
                     /locus_tag="VF402_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7799749.1"
                     /translation="MSHLVIDGVEIPEKLIRDEMPNHPAATSAQAYAAAGHALAIKAL
                     LLNRAGELGLEPQPAADGAGRFETEEEALIRQLFDRELAPETPTDAECQRFYDSRPDS
                     FLTPALYEASHILLLESARDIADDLMAQLQAEPSGFGGLARALSQCPSGRIGGSLGQM
                     QSGDLVAEVEAVLLSMPVGGVHPEPVRSRFGWHILRLDRLITPQRLPLEAVKDRIRLH
                     LESRAWIAASAKYVGELVLQAKERGIAFRLGETGDVAGPSLSLGALVDDERLADRIGP
                     WLEAVDPDLARRVAASAAAQQAALPEFVRREIRDFLDHADDEAFTQLVSAAQGADDPL
                     LASVRTILKSRLGAPRRTLNIRRKA"
     gene            3281..3844
                     /locus_tag="VF402_05330"
     CDS             3281..3844
                     /locus_tag="VF402_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7799750.1"
                     /translation="MAQPPATEIESLPPELVHEPLNWLFAEHYRHRQLCKIIEHIARS
                     EAFDADRIVAALRFLRQDMPLHVRDEEEDLFPLMRRRAQPDDDLERILGDLGADHHAD
                     AERVQFLIEGLEAALNRKTAPASDAGLRPLLLIFVAHERRHVALENAIVLPIARLRLT
                     PEDLRALSRRLAARRGVQLPEDGPQIL"
BASE COUNT          596 a         1318 c         1300 g          650 t
ORIGIN      
        1 ccgctatctc ggcgtcctgc tctacgacgc cgaccgcatc gaggcggccg cgtcggtcga
       61 cgacaaggat ctttatcagg cccagctcga cgtcttcctc gacccgcacg atcccgacgt
      121 catccgtcag gcgcgcgccg acgggatcgc ggaggcgtgg ctggaggccg cgcgcaacag
      181 cccggtctat aagatggcga tggactggaa gatcgccttc ccgctccatc ccgaataccg
      241 gacgctgccc atggtctggt acgtgccgcc gctgtcgccg atccagtcgg cggcctcggc
      301 gggcgcgctg gagatggacg gcgagatgcc ggacgtccat tcgctgcgca tcccggcgcg
      361 ctatctggcg aacctgctga ccgccggcgt cgaggcgccg gtcatcgcgg ccctgcaacg
      421 catgatgacg atgcgcatgt tcatgcgcgc caggacgatc gagggccgca tcgacgcgcc
      481 gctggccgaa aaggtcggcc tgtcggtcgc ccagatcgag gacatgtacc gcatcatggc
      541 catcgccaat tacgaggatc gcttcgtcat cccgaccacg caccgcgaag ccgccgagga
      601 cgcctatgag ctgcgcggca attgcggctt caccttcgga aacggctgtt cgggcggcca
      661 gaccgagttc ggccttttcg gcaagatcaa gaaaccggcc gccgcgccgc tggcgaagga
      721 ggcgtaatca tggccctgac ctacagagcc ctggcgcaac tgctgaccta tccgaccgcg
      781 gatatgcagt cgctggctgc ggacatcgcc cgcgtcctct ccggcgaggg cctgctgcgc
      841 gccgacctga tcgacggcgt gtcggcgctg gcgcgtcatc tggcggcggc ggatatctac
      901 gacgttcagg aagcctatat cgacctgttc gaccgctcgc gctcgctgtc cctgaacctc
      961 tacgagcatg ttcacggcga aagccgcgag cgcggtcagg ccatggcggc gctcgtcgaa
     1021 ctgtacgcca gccggggcct cgaactggac gcccgtgagt tgccggacta tctgccggtc
     1081 tttctcgaat tcctgtcgac cctgtcccag ccggaggccg cgtcgttgct gggcgaagcc
     1141 gtgcatgtgc tcgaggcgct gtcgcaacgg ctcgggaaac gcgaaagtcc ctatcgggcc
     1201 gttttcgacg cgctggtcgc gctggccggg gtcagggccg aagcgcaggt tctggcggcc
     1261 ttgctggccg agcctgagga taatcccgac gatctggacg cgctcgaccg gcaatgggcg
     1321 gagcaggccg tgaccttcgg gccgggcgaa gccggttgtc cgaaggccga ggccctcgtc
     1381 gccgccatgc aggcggatga acccgcgccg cgccgcgccg ccggaggagc agactaatgg
     1441 aaacgatcaa cagcctcctg ttcggggttt atccctacat cgccctgtcc gtgctggtca
     1501 tcggctcggt cctgcgcttc gaccgcgagc aatacacatg gcgctcaggg tcgtcgcagc
     1561 ttttgcgccg caagcagctc gtcgccggtt cggtcctgtt ccatctcggc gttctggtca
     1621 ttctgggcgg ccatatggtc ggcctgctga cgccgatcgc ggtgttcgag tatctcggca
     1681 ttccccacgg cgccaagcag gtgctggcca tggtcgcggg cggcgtcgcc ggggtgttcg
     1741 ccctggccgg ggcgctgctg ctgctgcacc ggcggctgtt cgacgcccgc gtccgccaca
     1801 attcgacgtt cggcgacacc gcgatcctca tcgtgctgac ggtgcagctc tgcctcggtc
     1861 tggcgaccat tcccctgtcg gcgcaacatc tggacggcag cgaaatggtc aagttcatga
     1921 actgggcgca gggcgtcttc acgttcaacc gtcaggcggc gggctatgtc gccgacgttc
     1981 cgtgggtctt caaggcgcac ctgatcctgg gcatgaccat cctgctggtg tttccgttca
     2041 cccgcctcgt ccacatgctg tcggcgccgg tctggtacct gaaccgcagg ggctggcaac
     2101 tggtgcggac gcgccgcgac atcgccgcgc tgcggaggaa atgagccatg agccatctcg
     2161 tcatcgacgg cgtcgaaatc ccggaaaagc tgatccgcga cgaaatgccg aaccacccgg
     2221 cggcgacgtc ggcgcaggcc tacgccgcgg cgggtcacgc cctggcgatc aaggcgctgc
     2281 tgctcaaccg ggccggtgag ctggggctgg agccgcagcc cgccgccgac ggcgcgggcc
     2341 ggttcgagac ggaggaggag gcgctgatcc ggcaactgtt cgaccgggag cttgcgccgg
     2401 agacgccgac cgacgccgag tgccagcgct tctacgacag ccgcccggac agcttcctga
     2461 cgcccgcgct ctacgaagct tcgcacatcc tgctgctcga aagcgccagg gacatcgccg
     2521 acgacctgat ggctcaatta caggcggagc cgtcgggttt cggcgggctg gcgcgggcct
     2581 tgtcgcaatg cccgtccggc aggatcggcg gctcgctggg gcagatgcag tccggcgatc
     2641 tggtggcgga ggtcgaggcg gtcctgctgt ccatgccggt tggcggcgtc catcccgaac
     2701 cggtccggtc gcggttcggc tggcatatcc tgcgtctcga ccgcctcatt acgccgcagc
     2761 ggctgccgct ggaggcggtg aaggaccgta tccgcctgca tctggagagc cgggcgtgga
     2821 tcgccgcgtc cgcgaaatat gtgggcgagc tggtgcttca ggcgaaagaa cgcggcatcg
     2881 cgttccggct gggcgaaacg ggcgatgtcg ccggacccag cctgtctctg ggcgcgctgg
     2941 tggacgatga gcgtctggcc gaccgcatcg gtccgtggct cgaagccgtc gacccggatc
     3001 tggcccggcg cgtcgccgcc agcgcggccg cgcaacaggc cgccctgccc gaattcgtcc
     3061 ggcgggaaat ccgggacttt ctcgaccacg ccgacgacga ggccttcacg cagctcgttt
     3121 ccgcggcgca gggcgcggac gatcccttgc tggccagtgt gcgcaccatc ctgaagtcgc
     3181 ggctgggcgc gccccgccgc acgctgaaca tccggcgcaa ggcctgaaga cgggccttcg
     3241 gaagccgatg gccgaacctc gatcaacgga gtcgacatcc atggcgcagc cgcctgcaac
     3301 cgaaattgaa tccctgccgc cggagctggt tcatgagccg ctgaactggc tgttcgccga
     3361 gcattaccgc caccggcaac tgtgcaagat catcgagcat atcgcccggt cggaagcctt
     3421 cgacgcggac cggatcgtgg ccgccctgcg cttcctcagg caggacatgc ccctgcatgt
     3481 ccgcgacgag gaggaggacc tgtttccgct catgcgccgc cgcgcccagc ccgacgacga
     3541 tctggagcgc atcctcggcg atctcggcgc cgatcaccat gccgacgcgg agcgcgtgca
     3601 gtttctgatc gaggggctgg aagccgccct gaaccgcaag accgcgcccg cgtcggacgc
     3661 tgggctgcgg ccgctcctgc tgatcttcgt cgcgcatgag cgccgtcacg tggcgcttga
     3721 gaacgccatc gtcctgccca tcgcccggct gcggctgacg cctgaggatt tgcgggcctt
     3781 gagccgtcgg ctggcggcca gaagaggcgt ccagctgccg gaagacggcc cgcaaatcct
     3841 ttgagcggcg acatgtctta gaag
//
LOCUS       DASVLL010000318         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_181547,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000318 DASVLL010000000
VERSION     DASVLL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181547"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF402_05335"
     CDS             complement(<1..258)
                     /locus_tag="VF402_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7799751.1"
                     /translation="MIERRAFDTLGAANHGWLDAHHHFSFADYYDPARMQWGGLRVWN
                     DDTIAPGTGFPPHPHADMEIVTYVREGAITHQDSLGNTGRTE"
     gene            373..1287
                     /locus_tag="VF402_05340"
     CDS             373..1287
                     /locus_tag="VF402_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7799752.1"
                     /translation="MMRLPDFEAWAIFAKVAQSGSFVAAAQELNLSQATVSKAVTRLE
                     RKLGTRLFHRTSRRLSLSDSGQGALEAAARLLAAGEALEAEASRDAARPRGVVRMTAP
                     MSFGVRHLGALLAEFFELYPDITVELTLSDRFIDLVEAGVDLALRIAELEDSSLLARH
                     LAPVRVLPVASPDYFARRGRPEHPRELSEHTCLIYTNTRAAGVWRFRRGARERYAVTV
                     TGPLRVDNADVLRDLLLQGAGIAMQPDFLAFEDVRAGRLARALPDWRGPELALCLLTP
                     PGATRPARVAALIAFLTERIRASDWAHG"
     gene            complement(1398..1919)
                     /locus_tag="VF402_05345"
     CDS             complement(1398..1919)
                     /locus_tag="VF402_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEX7799753.1"
                     /translation="MIPGMAALPPPFGRGALTAWRIDDRRHAAAWDSGEGAYRVGGRW
                     NSRGVRAVYCSLDPAAAILEVAVHKGFRALDIVPHVLTALRIDDPAAVHVVDPAMIPN
                     PNWLTPGIPGAGQQAFGDALLAAHALILIPSAVSRHSWNLIFDRDRARGAYACLGQEA
                     FALDTRLHPPQTA"
     gene            complement(1916..2404)
                     /locus_tag="VF402_05350"
     CDS             complement(1916..2404)
                     /locus_tag="VF402_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEX7799754.1"
                     /translation="MDTLDIGPKNVPPQGEMDMVVGLLGGDALFARPVRDRLDAHEII
                     LSGFPNKALRSLVRNVPVMQQPDVMEKALGISLRTFQRRSKADAVRPLSQEQSGRTWK
                     FAEILARATRILGSQTEAEAWLSRPATGLNQQRPIDLLATTAGVEIVEDHLERLEYGV
                     YA"
     gene            complement(2475..3881)
                     /locus_tag="VF402_05355"
     CDS             complement(2475..3881)
                     /locus_tag="VF402_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEX7799755.1"
                     /translation="MKPLLTALFAIAAAPLTAHAEVRVDAPRGIGDAVLHAEAFGAKG
                     DGVTDDTRALQAAIDATADKAGTLVLKPGTYLTGALFLKSHMALRIDKGVTLTGAQDL
                     KAYPRLPSRVAGIEMTWPVALINVYKQTDVKIYGDGIIDGDGKVFWDAYWKLRNAYQP
                     RGLRWASDYDAERARLMLVYGARRVEIGNGSLDNPLHLKRSGFWTVQVTYSDNIKVSG
                     IEVRNNIGGFGPSTDGVDIDSCHEVLVENADIDNHDDDIVIKSGRDADGLRVNRPSYN
                     IVVRNSIIRAGAAGIAFGSETSGGIHDVEVYGLKVVGPTGNGILLKSAHTRGGDMYNL
                     DIHDMRISGVETAIRADLNWNPAYSYASIPADVKNPPEIWKILATPVPHDEGLPHYHD
                     VHIHNIEADNVRAAIRFEAYPDAPIRDIDLDHIHIKAKTAGEIIHADGIHFTDTSIDA
                     ADGSHVRLEDAKDVTGLN"
     gene            complement(3878..>4522)
                     /locus_tag="VF402_05360"
     CDS             complement(3878..>4522)
                     /locus_tag="VF402_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799756.1"
                     /translation="AWNPVTGAAQVATGEITLAPYESRVFATGSGAAPPAPTQTLTLQ
                     TGWTLTAPDGKAQPVSDFGSWTRDPALLHYSGSLTYSRTLRLTPAQTKGHVMLDFGAG
                     QPVPPPKRAMGSQALLDPPVREAAEIFVNGRRVGAVWTSPFTLDLSGYVKAGDNRLEA
                     RVSNTAVNALSGRPAPDYGPLTARYGERFSPQGMTNLVPLPSGLMTAPKLEIAP"
BASE COUNT          689 a         1422 c         1614 g          797 t
ORIGIN      
        1 ctcggtgcgg cccgtattgc cgaggctgtc ctgatgggtg atcgcgcctt cgcgcacata
       61 ggtgacgatc tccatgtcgg cgtgcgggtg cggcggaaag ccggttcccg gcgcgatcgt
      121 gtcgtcgttc cagacgcgca ggccgcccca ttgcatgcgc gccggatcgt aatagtcggc
      181 gaaggaaaaa tggtgatggg cgtcgagcca gccgtgattg gcggcgccca gggtgtcgaa
      241 ggctctgcgt tcgatcatgt cggtctctcc ataggctgga acgcggcggg cgttccgtgt
      301 tgagcgcact atggacttgc ggcatcgaac gtgaaataga aatgatggaa aaccattctt
      361 tccaaaagcg acatgatgag actgccggat ttcgaagcct gggccatttt cgccaaggtc
      421 gcgcaaagcg gctcgttcgt cgccgccgcg caggagctga acctgtcgca ggcgacggtc
      481 tccaaggcgg tgacgcggct ggagaggaag ctggggacgc gcctgttcca ccgcacctcg
      541 cgccgcctgt cgctgagcga cagcgggcag ggcgcgctgg aggccgccgc gcggctgctg
      601 gcggcgggcg aggcgctgga ggcggaggcg tcgcgcgacg cggcgcggcc gcgcggcgtc
      661 gtgcgcatga ccgcgccgat gtcgttcggc gtgcgccatc tgggcgcgct gctggcggaa
      721 tttttcgagc tttatccgga tattacggtc gaattgacgc tgagcgaccg gtttatcgac
      781 cttgtcgagg cgggcgtcga tctggccctg cgcatcgccg aactggaaga ttccagcctg
      841 ctggcgcggc atctggcgcc ggtgcgcgtg ctgccggtgg cgtcgccgga ctatttcgcg
      901 cggcgcggac ggcctgagca tccgcgcgag ctgagcgaac atacgtgcct gatctatacc
      961 aacacccgcg ccgccggggt ctggcgcttc cggcgcggcg cgcgggagcg ctatgcggtc
     1021 acggtgacgg ggccgttgcg cgtcgataac gccgacgtgc tgcgcgacct tttgctgcag
     1081 ggcgcaggga tcgccatgca gccggacttt ctggccttcg aggatgtgcg cgccgggcgg
     1141 ctggcgcgcg ccttgccgga ctggcgcggg cctgagttgg ccttgtgcct cctgacgccg
     1201 ccgggcgcga cccggcccgc gcgcgtcgcg gcgctgatcg ccttcctgac cgagcgcatc
     1261 cgcgcctcgg actgggcgca tggctagcgt ttccccaaaa agcagctcca ctttttggat
     1321 aaggaagagc gacaaaacaa agacttagag cgccgatctg atgcaatcag atcgaaaagc
     1381 gctctaaagg ctccggttca ggccgtttgc ggcggatgca gccgggtgtc gagcgcgaag
     1441 gcttcctgac cgaggcaggc ataggcgccc ctggcgcggt cgcggtcgaa gatcaggttc
     1501 cagctatggc gcgacacggc gctggggatc aggatcagcg catgggcggc cagcagggcg
     1561 tcgccgaacg cctgctgacc ggcgccgggg atgccgggcg tcagccagtt cgggttcgga
     1621 atcatggccg gatcgaccac atgcacggcg gcgggatcgt cgatccgcag cgcggtcagc
     1681 acgtgcggca cgatgtcgag cgcgcggaaa cccttatgca cggcgacttc gagaatggcg
     1741 gcggcgggat caagcgaaca atagacggcg cgcacgccgc gcgaattcca gcgcccgccg
     1801 acgcgatagg cgccttcgcc gctgtcccat gcggcggcgt ggcggcggtc gtcgatgcgc
     1861 catgcggtca gcgcgccgcg tccgaacggc ggcggcaggg ccgccatgcc ggggatcacg
     1921 cgtaaacgcc gtattcgagc cgttcgaggt ggtcctcgac gatctccacg cccgccgtgg
     1981 tggccagaag gtcgatgggg cgctgctggt tcaggccggt ggcgggacgg ctgagccacg
     2041 cctcggcctc ggtctgcgag cccagaatgc gcgtggcgcg cgccagaatc tcggcgaatt
     2101 tccaggtgcg cccgctctgc tcctgactca gcgggcgcac ggcgtccgcc ttgctgcggc
     2161 gctggaaggt gcgcaggctg atgccgagcg ccttttccat cacgtccggc tgctgcatga
     2221 ccggcacgtt gcgcaccagc gagcgcaaag ccttgttggg aaagcccgac aggatgatct
     2281 cgtgcgcgtc cagccggtcg cgcaccggcc gcgcgaacag cgcgtcgccg ccgagcaggc
     2341 cgacgaccat atccatttcg ccttgcggcg ggacgttctt cggcccgatg tcgagcgtgt
     2401 ccatgcaacg cctccgtcat gtgtcgctct acagatgaca catgacgccg gaaatttcaa
     2461 gtctccgggg ccggtcagtt cagcccggtg acgtctttgg cgtcctccag ccgcacatgc
     2521 gagccgtcgg cggcgtcgat gctggtgtcg gtgaagtgaa tcccgtcggc gtggatgatc
     2581 tcgccggcgg ttttcgcctt gatgtggata tggtcgaggt cgatatccct gatcggcgcg
     2641 tcgggatagg cttcgaagcg gatggcggcg cggacgttgt cggcctcgat attgtggata
     2701 tggacgtcgt gatagtgcgg caggccctcg tcgtgcggca ccggcgtggc gaggatcttc
     2761 cagatttccg gcgggttttt cacgtcagcg gggatgctgg cgtaggaata ggccgggttc
     2821 cagttcaggt cggcgcggat ggccgtctcg acgccggaaa tccgcatgtc gtggatgtcg
     2881 agattgtaca tgtcgccgcc gcgcgtgtgc gccgatttca aaaggatgcc gttgcccgtc
     2941 gggccgacca ccttcaggcc atagacctcg acatcgtgga tgccgccgga ggtttccgag
     3001 ccgaaggcga tgcccgccgc gcccgcgcgg atgatcgagt tgcgcaccac gatattgtag
     3061 ctgggccggt tgacgcgcag gccgtcggcg tcgcggcccg acttgatcac gatgtcgtcg
     3121 tcgtgattgt cgatgtcggc gttctcgacc agcacctcat ggcaggaatc gatgtcgacc
     3181 ccgtcggtcg acggcccgaa gccgccgata ttgttgcgca cctcgatgcc cgacaccttg
     3241 atattgtcgg aataggtgac ctgcacggtc cagaagccgg agcgcttcag atgcagcggg
     3301 ttatcgaggc ttccattgcc gatctcgacg cggcgcgcgc catagaccag catcagccgc
     3361 gcgcgctcgg cgtcgtagtc ggacgcccag cgcaggcccc tgggctgata ggcgttgcgc
     3421 agcttccagt aggcgtccca gaacaccttg ccgtcgccgt cgatgatccc gtcgccatag
     3481 attttcacat cggtctgctt atagacgttg atcagcgcca ccggccaggt catctcgatc
     3541 cccgccacgc gcgagggcag gcgcggatag gctttcagat cctgcgcgcc ggtcagggtc
     3601 acgcccttgt cgatgcgcag agccatgtgc gattttaaga acagcgcgcc ggtcaggtag
     3661 gttccgggct ttaagaccag cgtgcccgcc ttgtccgccg tggcgtcgat ggcggcttgc
     3721 agcgccctgg tgtcgtcggt gacgccgtcg cccttggcgc cgaaagcctc ggcgtgcagc
     3781 acggcgtcgc cgatgccgcg cggggcgtcg acacggactt cggcatgggc ggtgagcggg
     3841 gcggcggcga tagcgaacag ggcggtgaga agcggtttca cggggcgatc tccagtttcg
     3901 gggcggtcat cagaccggac ggcaggggga cgaggtttgt catgccttgc ggcgaaaagc
     3961 gctcgccgta cctggcggtc agcgggccgt aatcgggcgc cggacggccg gacagcgcat
     4021 tgacggcggt gttgctgacg cgcgcctcca gccggttgtc gcccgccttg acgtagccgc
     4081 tcaggtccag cgtgaacggc gaagtccaga ccgcgccgac gcggcggccg ttgacgaaga
     4141 tttcggcggc ttcgcgcacc ggcggatcga gcagggcctg cgagcccatc gcgcgtttcg
     4201 gcggcgggac cggctggccc gcgccgaaat cgagcatgac atggcccttg gtctgcgcgg
     4261 gcgtcaggcg cagcgtgcgg ctgtaggtca ggctgccgga ataatgcaac aaagccggat
     4321 cgcgcgtcca ggagccgaaa tcgcttaccg gctgcgcttt cccgtccggc gctgtcaacg
     4381 tccagccggt ttgcagcgtc agggtctgcg tcggcgcggg cggcgcggcg ccgctgcccg
     4441 ttgcgaagac gcgcgattcg tagggcgcca gcgtgatctc gcccgttgca acctgcgccg
     4501 cgccggtgac ggggttccac gc
//
LOCUS       DASVLL010000319         4840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_181779,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000319 DASVLL010000000
VERSION     DASVLL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181779"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="VF402_05365"
     CDS             <1..84
                     /locus_tag="VF402_05365"
                     /inference="COORDINATES: protein motif:HMM:NF018531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX7799757.1"
                     /translation="CARGDGGVIERKVHQGRSTFFCPVCQV"
     gene            373..642
                     /gene="rpsT"
                     /locus_tag="VF402_05370"
     CDS             373..642
                     /gene="rpsT"
                     /locus_tag="VF402_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7799758.1"
                     /translation="MANNPGARKAIRKIARRTEVNTARRSRVRTFVRKFEEAIVKGDA
                     AVAKAAFVEAQSELMRAVSKGVVHKNTGARKVSRLAARLKKLSLA"
     gene            1429..2832
                     /gene="dnaA"
                     /locus_tag="VF402_05375"
     CDS             1429..2832
                     /gene="dnaA"
                     /locus_tag="VF402_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7799759.1"
                     /translation="MPGMTSWSPILPEAVWSKVATILRRELGEGPYNSYVAPSSVKAG
                     PYGDPVLVTPTVYARDWIARNALRRAQELWSEHDGEARVLDVKARIEFDDAACAASAP
                     PPAPEPAPALVPAAQGKVTGLQERLTFDSFVAGRGNEFAYSMSRQVASWADGHFNPVF
                     LHGPYGYGKTHLLNAIGWEAQARRPEAKIVYLTAEKFTSTFVKAIMDRSTAAFKDEVR
                     GADLLLVDDVHFIGGKTSSQEELFHTLTTLIENNRRVVFTADRPPSQLTEIEARLRSH
                     LSSGLVCALDVADQTLRMGIIERKLDQLQQRLGAGMAHPRADVLHFLAERVPGSIREL
                     EGAVNTLVASAGPRLGSLTLEEAMALLQPNLKVAVERRVTVDEIQKLTADHFGLKQAD
                     LLSERRTRSVARPRQVAMWLCKQHTTRSYPDIGRRFGGRDHTTVLHAVKKVEELLTSD
                     DQIARDVEALTRKLRNA"
     gene            3033..4154
                     /gene="dnaN"
                     /locus_tag="VF402_05380"
     CDS             3033..4154
                     /gene="dnaN"
                     /locus_tag="VF402_05380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7799760.1"
                     /translation="MQFTIERGALLKALGHVQNVVERRNTIPILSNVLLSAEGHTVSF
                     SATDLDMEIVDTAEAIVNAPGQITAPAHTLYEIVRKLPEGADVELKFAAGDDPRLAVS
                     AGRSRFALPVLPAGDFPIMSNENDGVTFAILRDDLKRLIDKTRFAVSTEETRYYLNGM
                     FLHTLSEDGSNYLRAVATDGHRLALAEMPAPDGAIGGPGVIIPRKTIDQARRLLDDGT
                     GYVDLSISPAKIRFQFGEASLTSKIIDGSFPDYGRVIPKNNDKTMMIDTGVLAKAVDR
                     VSTISAEKSRSVKMAIEPGRLTLSVRNIEAGQAVEEAEIDYDNEALDIGFNARYILDV
                     MGQIGGDTVELRFSDASSPTLVLDPVDNGVQYVLMPLRV"
     gene            4329..>4840
                     /locus_tag="VF402_05385"
     CDS             4329..>4840
                     /locus_tag="VF402_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7799761.1"
                     /translation="MTPANHALQHLSLTEFRSYERLDFDVAGRSVYLYGPNGAGKTNL
                     LEAISVLNPGKGLRGAAFADLGRRLPDEVRGRPWGVAARFYAGEDETRLATGSDPRDL
                     NKRAVRIDGETVPASRLLEHVRLIWLTPAQDRLFIEARAERLRFFDRLVYAADPSHAA
                     SVSAYERALR"
BASE COUNT          803 a         1575 c         1587 g          875 t
ORIGIN      
        1 tgcgcgcgcg gcgacggcgg cgtgatcgaa cgcaaggtcc atcaggggcg ctcgaccttc
       61 ttctgcccgg tctgccaggt ctgagccagg gtttttgttt tgccgccctt atccgaaacg
      121 tggagccgct tttgggggaa acgccccgga cggcggcgtc gcccgtcctg ttcttttccg
      181 ggcttgccgc ggttaagtct tccgccttcc acctttgtgc ataaaattct cgcggcgggt
      241 tcagattcag ggttggcgcg cattgaccgc gccgggatca tcggttatat cccgccccct
      301 tgaaattcat gtctccgctc cgggggtccg gcgcggcggc tttcctttta cactcgtcag
      361 tttaggtgat gaatggccaa taatcccggc gcccgtaaag cgatccgcaa gatcgcccgc
      421 cgcaccgaag tcaatacggc tcgccgctcg cgcgtccgta ccttcgtgcg caagttcgaa
      481 gaagcgatcg tcaagggcga cgccgccgtc gccaaggcgg ctttcgtcga ggcgcagtcc
      541 gagctgatgc gcgcggtctc caagggcgtg gttcacaaga acaccggcgc gcgcaaggtt
      601 tcgcgcctgg cggcccgcct gaaaaagctg agcctggcct aagggccgct tcggcgccgt
      661 ccggcctggc cggaaaacgc cgttcgacag tcgagctttc cggattttaa gcccacgcac
      721 gcgatggcgg gcgtgggctt tgtcgcgcct ggtctccggc ggagacgggc ggggcggcga
      781 cttccttaaa tttgccttaa tttctctttg cgggcgaacg gcttaccgga gtcaacagaa
      841 atatccgacc ttggggataa tttcccgcat tgaccggcgc gagcagcgcg ctaatttggg
      901 tctctccccg cccaactgct cccggaacgg agcatctggg cggcgttcgg aaccggcccg
      961 cggtctgtgt ttcaggggct tcaaacccct ggcgcgcatc ggcggggcgg cgttcggaaa
     1021 ccggggtcgc acccggattg gcggcgtcct gtcggacgct gcgttccgaa cggcgtcggc
     1081 gcggtggacc ttccaccgtc tgagatgatc cgggcgcgag agttcagctc taggcaatgg
     1141 gtggcgaaac ccgtttttcg tctgtccggc gggtgtcgat ccgcggggcg gggttccttg
     1201 tcccgatgcg tcgggggaag gggcggtcct gttgctgtgg atggcgtggc ggatttcgcg
     1261 tccggcgcga acggggagtc gaaaatgggg caggctggat cggcgggaaa gacgccggtt
     1321 acgaacagga cgatgtaaag gcggcggttc gcggcgttgg tcgcgggggc cgataagtgg
     1381 agtggcgtgg tgaacgggtt aagggcacaa gatcggggcg aaacaacaat gccgggcatg
     1441 acaagctggt cgccgatcct acctgaagcg gtatggtcca aggtggcgac gattctgcgc
     1501 agggagctgg gcgaagggcc gtacaattcc tatgtggcgc cgtcgagcgt gaaggcgggg
     1561 ccttacggcg atccggtgct ggtgacgccg accgtttacg cgcgcgactg gatcgcgcgc
     1621 aacgccctgc gccgcgcgca ggaattgtgg agcgagcatg acggcgaggc gcgcgttctg
     1681 gacgtcaagg cgcgcattga gttcgatgac gccgcctgcg ccgcgtccgc gccgccgccc
     1741 gcgcccgaac cggcgcccgc gctcgtcccg gcggcgcagg gcaaggtcac cggcttgcag
     1801 gagcgcctga ccttcgacag tttcgtcgcc gggcgcggca acgagttcgc ctattcgatg
     1861 tcgcgtcagg tcgccagttg ggccgacggg catttcaatc cggtcttcct gcacggcccc
     1921 tacggctacg gcaagaccca ccttctcaac gccatcggct gggaggcgca ggcgcgccgc
     1981 ccggaggcga agatcgtcta tctgacggcg gagaagttca cctcgacctt cgtgaaggcg
     2041 atcatggacc gctcgaccgc cgccttcaag gacgaggtgc gcggggccga tcttctgctg
     2101 gtcgacgacg tgcatttcat cggcggcaag acctcgtcgc aggaggagct gttccacacc
     2161 ctgacgacgc tgatcgagaa caatcgccgc gtcgtcttca ccgccgaccg tccgccgtcg
     2221 caactgaccg agatcgaggc gcgcctgcgt tcgcacctgt cgtcgggtct ggtgtgcgcg
     2281 ctggacgtgg cggatcagac tttgcgcatg ggcatcatcg agcgcaagct ggaccagttg
     2341 caacagcggc tcggcgccgg gatggcgcat ccgcgcgccg acgttttaca ttttctggcc
     2401 gagcgtgtgc cggggtcgat ccgtgagctt gaaggcgcgg tgaacaccct ggtggcgtcc
     2461 gctgggccgc gtcttgggag tttgactttg gaagaagcta tggccttgct gcaaccgaac
     2521 ctgaaggtcg ccgtcgagcg tcgcgtgacg gtggacgaga ttcagaagct gacggcggac
     2581 catttcggtt tgaaacaggc ggaccttttg tcggagcgcc gcacgcgctc ggtggcgcgt
     2641 ccgcgtcagg tggcgatgtg gctgtgcaag cagcacacga cgcgctccta tcccgacatc
     2701 gggcgccggt tcggcggccg cgaccacacg acggtgctgc acgcggtgaa gaaggtcgag
     2761 gagctgctga cgagcgacga ccagatcgcg cgcgacgtcg aggcgctcac ccggaaactc
     2821 aggaatgcgt aagggggttt ggggccaatt ggccccaagc cttccctttt cttatccttc
     2881 acccgccttt cgggcgggcg aaggataaga aagataaaga tgtggggtcg attgacccca
     2941 cgccccgttt cgtctttctt gaattttgcg gatgatcgtc taatgtccgc ctcccggccc
     3001 tcgccgatct tatcccgact caggactgca agatgcaatt caccatcgaa cgcggcgctc
     3061 tgttgaaggc cctgggccat gtgcagaacg tggtcgaacg ccgcaacacc atccccatcc
     3121 tgtccaacgt ccttttgtcc gccgaaggcc atacggtctc gttctcggcc accgatctcg
     3181 acatggagat cgtcgatacc gccgaggcca tcgtcaacgc gccgggccag atcaccgcgc
     3241 ccgcgcacac cctctatgaa atcgtgcgca agctgcccga aggcgccgat gtcgagctga
     3301 aattcgccgc cggcgacgat ccgcgcctcg ccgtctcggc gggccgctcg cgcttcgccc
     3361 tgccggtgct gcccgccggc gactttccga tcatgtcgaa cgagaatgac ggcgtcacct
     3421 tcgccatcct gcgcgatgac ttaaagcgcc tgatcgacaa gacgcgcttc gccgtctcga
     3481 ccgaggagac gcgctactat ctcaacggca tgttcctgca cacgctgagc gaggacggct
     3541 cgaactacct gcgcgccgtc gccaccgacg gccaccggct ggccctcgcc gaaatgcccg
     3601 cgcccgacgg cgcgatcggc ggccccggcg tgatcatacc gcgcaagacc atcgaccagg
     3661 cgcgccgcct gctcgacgac gggaccggct atgtcgacct aagcatcagc ccggccaaga
     3721 tccgcttcca gttcggcgaa gcctcgctga cctcgaaaat catcgacggc tccttccccg
     3781 actatggccg cgtgatcccc aagaacaacg acaagacgat gatgatcgac accggcgtgc
     3841 tggccaaggc cgtcgaccgc gtctcgacca tctcggcgga aaagtcgcgc tcggtgaaga
     3901 tggcgatcga gccgggccgc ctgaccctgt cggtgcgcaa tatcgaagcc ggtcaggccg
     3961 tcgaggaagc cgagatcgac tacgacaacg aagcgctcga tatcggcttc aacgcccgct
     4021 atatcctcga cgtcatgggc cagatcggcg gcgacaccgt cgaactgcgc ttctccgacg
     4081 cctcctcgcc gaccctggtg ctcgatccgg tcgacaatgg cgtgcagtac gtgctgatgc
     4141 cgctgcgggt gtaagaccgt tatgaaggat tcccccttct cctcccctgc gaagcggggg
     4201 agggggacca cgaagtggtg gagggggctt tcggatatct gcgctggccc cctccgtctc
     4261 gacgcgctac gcttgctcga cacctccctc ggaacgaaag ttcccgcttt gcaggggagg
     4321 ggattatgat gacgcccgcc aaccacgccc tgcaacacct gtccctgacc gagttccgtt
     4381 cctatgaacg gctcgacttc gacgtggccg ggcgcagcgt ctatctctac ggccccaatg
     4441 gcgcgggcaa gaccaacctg ctggaagcga tcagcgtcct caatccgggc aagggcctgc
     4501 gcggcgccgc cttcgccgat ctgggccgcc gtctgcccga cgaagtgcgc gggcggccgt
     4561 ggggcgtggc ggcgcgcttt tacgcaggcg aggacgagac gcgcctcgcc accggctccg
     4621 acccgcgcga cctgaacaag cgcgccgtgc gcatcgacgg cgaaaccgtc ccggcgtcgc
     4681 gcctgctgga gcatgtgcgg ctgatctggc tgaccccggc gcaggaccgt ctgttcatcg
     4741 aggcgcgcgc cgagcggctg cgcttcttcg accggctggt ctatgccgcc gacccgtcgc
     4801 acgcggcctc ggtcagcgcc tatgaacgcg ccttgcgcga
//
LOCUS       DASVLL010000320         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_342029,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000320 DASVLL010000000
VERSION     DASVLL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342029"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VF402_05390"
     CDS             <1..895
                     /locus_tag="VF402_05390"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799762.1"
                     /translation="RTTLDAYVTYQRTLGAWTVLPGLRLEQERVEVATDGGAASGFHH
                     VLPSLHLSRALGKGKLHIAYNRKIERPQITQYSPSIVYYSARSAYQGDPGLKSPRTDS
                     FEIGYDLNRGEALTGLTLYRRATRDLLATYSRDIGGDVLLTRDVNAGRARSTGAELSL
                     KAPLPHGFKYMLDVNLFSEKAPYYNGADGATHSRVTYTGNGSLEYDRADGEQYQISFA
                     VDGCRLSVQGYTAPTSHVDLNWSRPLTKKASLTLSVSDVFKGQDRVTVFTTPALKGRS
                     IRFDDERVWRVGLAWKFGGGA"
     gene            complement(901..1431)
                     /locus_tag="VF402_05395"
     CDS             complement(901..1431)
                     /locus_tag="VF402_05395"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7799763.1"
                     /translation="MKSIVRTAAIALAACAALAAAPSFAALKVGDKAPDFKLQAATNG
                     QVSTFSLAQALKSGPVVVYFYPKAFTSGCSLEAHEFSEAIPKFQARHVTVIGVSADDI
                     GTLQKFSEQTCQGKFPVAADAGAKVAKAYDAQMGGMAMANRISYIVGQNGKIAFVHDD
                     MNAATHVSSLLAAIGK"
     gene            1565..2527
                     /gene="fabD"
                     /locus_tag="VF402_05400"
     CDS             1565..2527
                     /gene="fabD"
                     /locus_tag="VF402_05400"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7799764.1"
                     /translation="MTLTPKTLAFVFPGQGSQSVGMGAELYENFATAREVFHEIDEAL
                     GQKLFALMKDGPEEQLTLTENAQPAIMAVSLAAARVLDKDFGIPCAKAKYVAGHSLGE
                     YSAHAAAGTFSLADTARLLKLRGQAMQRAVPVGVGAMAALIGKADLELAEKACEAGGA
                     AGVVVIANDNNAGQIVISGMKQAVELAVDKAKELGAKAMLLNVSAPFHCPLMQPAADE
                     MRDALAAVTIHAPVSTLIANVTARPVADKDEIAGLLVAQVTGRVRWRESVMWLAGEGQ
                     TTVFAELGAGKVLTGMGKRIAPEAGSVALNGAADFEAFAKELLS"
BASE COUNT          458 a          801 c          880 g          442 t
ORIGIN      
        1 gcgcaccacg ctggacgcct atgtcaccta tcagcgtacg ctcggcgcct ggaccgttct
       61 gccgggactg cgccttgagc aggagcgcgt cgaggtcgcg acggacggcg gcgccgcatc
      121 cggtttccat catgtgctgc cgtctctgca tctgtcgcgc gcgctgggca agggcaagct
      181 gcatatcgcc tataaccgca agatcgaacg gccgcagatc acgcaataca gcccgtccat
      241 cgtctattac agcgcgcgca gcgcctatca gggcgatccc ggcctgaaat cgccgcggac
      301 cgactcgttc gagatcggct acgacctgaa caggggcgag gcgctgaccg ggctgaccct
      361 gtaccgccgc gcgacgcgcg acctgttggc gacctacagc cgcgacatcg gcggcgatgt
      421 tcttctgacg cgcgacgtca acgccgggcg cgcgcgttcg acgggcgccg aactgtcgct
      481 caaggcgccg ctgccgcacg gcttcaaata catgctggac gtgaacctgt tttccgagaa
      541 ggcgccctat tataacggcg cggacggcgc gacccacagc cgcgtcacct ataccggcaa
      601 cggttcgctg gaatacgacc gcgccgacgg cgagcagtat cagatcagct tcgccgtcga
      661 cggatgccgc ctgagcgttc agggctatac ggcgccgacc agccatgtcg atctgaactg
      721 gtcgcgcccg ctcacgaaaa aagcgtcgct gaccctctcc gtcagcgacg tcttcaaagg
      781 acaggaccgc gtgaccgtct tcaccacgcc ggcgctgaaa gggcgctcga tccggttcga
      841 tgacgaacgg gtctggcgcg tcggcctggc ctggaaattc ggcggcgggg cttgaaagac
      901 ctatttgccg atggcggcca gcaggctgga gacgtgggtg gccgcgttca tgtcgtcgtg
      961 gacgaaggcg atcttgccgt tctggccgac gatgtaggaa atgcggttgg ccatggccat
     1021 gccgcccatc tgggcgtcat aggccttggc gaccttcgcg cccgcgtccg ccgccaccgg
     1081 gaacttgccc tgacaggtct gctcggagaa tttctgcaac gtgccgatgt cgtccgccga
     1141 cacgccgatc accgtgacgt gtctggcctg aaatttcggg atggcttcgg agaattcgtg
     1201 cgcctccagc gaacagccgc tggtgaaggc cttggggtag aaatagacga cgaccgggcc
     1261 ggacttcagg gcctgagcca gcgaaaacgt gctgacctga ccgttggtgg ccgcttgcaa
     1321 tttgaaatcg ggcgccttgt cgccgacctt gagcgcggcg aaggacggcg cggcggcgag
     1381 cgcggcgcag gcggcgagag cgatggcggc ggtgcggacg atggatttca tgagcgtgtc
     1441 tcctcggact gttgtgacgc tacgtcttat acgccgcggc cgcaagatgg gttacagctt
     1501 gcccaaaagc cgagtttggc ctaaccagcg gatcgttttc gcatagcttt caggattcgg
     1561 cattatgact ctgacgccta agactctggc cttcgtattt cccggacaag gctcgcaaag
     1621 cgtcggcatg ggcgcggagc tttatgagaa cttcgccacg gcgcgcgagg tctttcatga
     1681 gatcgacgag gcgctgggcc aaaagctgtt cgcgctgatg aaggacgggc cggaggagca
     1741 actgaccctg accgaaaacg cccagcccgc gatcatggcc gtgtccctgg ccgccgcgcg
     1801 cgtgctggac aaggatttcg gaatcccctg cgccaaagcg aaatacgtcg cgggccacag
     1861 cctcggcgaa tattcggcgc atgcggcggc ggggacgttc tcgctggccg acacggcgcg
     1921 gctgttgaag ctgcgcggtc aggcgatgca acgcgccgtg ccggtgggcg tcggcgcgat
     1981 ggcggcgctg atcggcaagg ccgacctcga actggcggaa aaggcctgcg aagcgggcgg
     2041 cgcggcgggc gtggtggtca tcgccaacga caacaatgcg ggccagatcg tcatttccgg
     2101 catgaagcaa gcggtggagc tggccgtgga caaggccaag gagctgggcg ccaaggcgat
     2161 gctgctcaac gtctcggcgc cgttccattg cccgctgatg cagccggcgg cggacgagat
     2221 gcgcgacgcg ctggccgccg tgacgatcca tgcgccggtc tccaccctga tcgccaacgt
     2281 caccgcgcgt ccggtggcgg acaaggacga gatcgcgggc ctgctggtcg cgcaggtgac
     2341 gggccgggtg cgctggcgcg aaagcgtgat gtggctggcg ggcgagggac agacgaccgt
     2401 tttcgccgaa ctgggcgcgg gcaaggtgct gaccggcatg ggcaagcgca tcgcgccgga
     2461 ggccggttcc gtcgcgctga acggcgccgc cgatttcgag gcgttcgcga aagagctgtt
     2521 gtcttgacgt cagctttcaa ctaaaccggc gcgctgattt caggagatat catgttcgat
     2581 c
//
LOCUS       DASVLL010000321         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_128680,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000321 DASVLL010000000
VERSION     DASVLL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128680"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VF402_05405"
     CDS             complement(<1..1487)
                     /locus_tag="VF402_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799765.1"
                     /translation="MRRSSGLAALAALAFAAPAFAAPQIAPQIAPQIAPMDATVRLRL
                     TPQTGALLEQLMTQGRTLRIDGQDVFGSGDKFLGGKIADGMAFVIVGMDDGDPRLARY
                     EAGFRKLARLTLDDKLTTWGIYYYMQALSKLKDAGRLEPCVDPQTLAALKAKLDWRSF
                     VDKDYRLIDLPNNYYGVAFSIAQLRHKFGWEDDSGMTALLDRTLAHYRTYSQYGFADE
                     TDGQGRFDRYSILLIGELAQRYIEAGLTPPQDVRDWLRESARLLLLRLNAEGDGFEYG
                     RSIGTYGETAFLEVLSAAATLNLLTPRERDAAYSFSAVAAQRYMDFWVDPATGSVNMW
                     DKGRKTDDYRGKNRILGENLSLARQYIYTDAEWNALGYRDVAPMTDLAAWRAAQPPLT
                     LTWFAKGDYDRALVTVIDGQRLFGLPLINGGEDQHMHSPYFPLPFSPGLISGAPDGAA
                     PQLIARFTLADGAQLEPLSYFRHVGARALPGGGAEAVYDQAQMDRL"
     gene            complement(1489..2742)
                     /locus_tag="VF402_05410"
     CDS             complement(1489..2742)
                     /locus_tag="VF402_05410"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX7799766.1"
                     /translation="MRLPDLLSVSFFAVMAALPAAAPALSAPVCDGAHGYAAAGQATF
                     LWRPDAMSAVRAGLAGDPAYADLLRDADAALRKGPWSVTDKTHATPGGDRHDYMSIAP
                     YWWPDPAQPNGEPYVNHDGRVNPERATNAFDRTRLGDMSEAVATLALAYHYSGRWDYA
                     DRAAQILRVWFLDAKTRMNPNMNYAQGVPGRAPGRSYGIIDAASFLPVTEAIGLLQPS
                     GALSAEDEAGLRTWFGDFADWMLTSDNGKTERNARNNHGIWYDLQLTDYALFAGRADL
                     AREVASAFARRRLEPEMAADGSLPQELARTRSFHYSTWTMQAVYDEAALARCVGVDLW
                     GWKAADGRSLRLATDFIARYAGHEGDWRWPEIAMNKDDLYEALLRAAWGFDDPALDAR
                     ADIYRKTYASARINLIYPPLPAKGH"
BASE COUNT          462 a          961 c         1009 g          471 t
ORIGIN      
        1 agccggtcca tctgcgcctg atcgtaaacc gcctcggcgc cgccgcccgg cagcgcgcgc
       61 gcgccgacat gccggaaata gctgagcggc tcaagctgcg cgccgtcggc cagcgtgaag
      121 cgcgcgatca gttgcggcgc cgcgccgtcg ggcgcgccgc tgatgaggcc gggcgagaag
      181 ggcaagggga aatagggcga atgcatatgc tggtcctcgc cgccgttgat cagcggcagg
      241 ccgaacagcc tctgcccgtc gatcaccgtg acgagggcgc ggtcatagtc ccctttcgcg
      301 aaccatgtca gggtcagcgg cggctgcgcc gcgcgccagg cggcgaggtc cgtcatcggc
      361 gcgacgtcgc gatagccgag cgcgttccat tccgcgtccg tgtagatata ttgccgggcc
      421 aggctgaggt tttcgccgag aatacggttt ttcccgcgat aatcgtcggt cttgcgcccc
      481 ttgtcccaca tattgaccga gccggtggcc ggatcgaccc agaagtccat atagcgctgc
      541 gcggcgacgg cgctgaagct gtaggccgcg tcgcgttccc gcggcgtcag gagattcagc
      601 gtcgccgccg cgctcagcac ttcgagaaag gccgtctcgc cataggttcc gatgctgcgg
      661 ccatattcga agccgtcgcc ttcggcgttg aggcgcagca gcagcaatct ggcggattcg
      721 cgcagccagt cgcgcacgtc ctgcggcggc gtcaggcccg cctcgatata acgctgcgcc
      781 agttcgccga tcagcagaat actgtaccgg tcgaaacgcc cctgcccgtc cgtctcgtcg
      841 gcgaaaccgt attgcgaata ggtgcgatag tgcgccagcg tccggtcgag cagcgcggtc
      901 atgccgctgt cgtcctccca gccgaacttg tggcgcagtt gcgcgatgct gaaggcgacg
      961 ccgtaatagt tgttgggcag gtcgatcagc cggtaatcct tgtccacgaa gctgcgccag
     1021 tcgagcttcg ccttcagcgc cgcgagggtc tgcggatcga cgcagggctc gagccgcccg
     1081 gcgtccttca gcttggacag ggcctgcatg taataataga tgccccaggt cgtcagcttg
     1141 tcgtcaagcg tcagccgcgc cagcttgcgg aacccggcct cgtagcgggc gaggcgcgga
     1201 tcgccgtcgt ccatgccgac gatgacgaag gccatgccgt cggcgatctt gccgcccagg
     1261 aacttgtcgc cggagccgaa cacgtcctgc ccgtcgatgc gcaaggtccg gccctgcgtc
     1321 atcaactgct ccagcagcgc gccggtctgc ggcgtcaggc gcagccggac ggtcgcgtcc
     1381 ataggcgcga tttggggcgc gatttggggc gcgatttggg gcgcggcgaa ggcgggcgcg
     1441 gcgaaggcca gcgccgccag cgccgccagg cctgaggagc ggcgcatgct aatgaccctt
     1501 cgccggcagg ggcggataga tcagattgat gcgcgccgag gcgtaggtct tgcggtagat
     1561 gtccgccctg gcgtcgagcg caggatcgtc gaagccccac gccgcgcgca gcagcgcctc
     1621 atagaggtcg tccttgttca tggcgatttc cggccagcgc cagtcgccct cgtgaccggc
     1681 gtagcgggcg atgaaatcgg tcgccagacg taaggagcgc ccgtcggcgg ccttccagcc
     1741 ccacagatcg acccccacgc atcgcgccag cgccgcttcg tcatagacgg cctgcatcgt
     1801 ccatgtgctg taatggaagg agcgcgtgcg cgccagttcc tgcggcagcg aaccgtccgc
     1861 cgccatttcc ggctccagac gacgccgcgc gaaggcggac gccacctccc tggccagatc
     1921 ggcgcgcccg gcgaacaggg cgtagtcggt cagttgcagg tcgtaccaga tgccgtgatt
     1981 gttcctggcg ttgcgctcgg tcttgccgtt gtcgctggtc agcatccagt cggcgaagtc
     2041 gccgaaccat gtgcgcaggc ccgcctcgtc ctcggcgctc aaggcgccgg aaggctgcaa
     2101 aaggccgatc gcctcggtca ccggcagaaa gctggcggcg tcgataatgc cgtaggagcg
     2161 ccccggcgcc cgccccggca cgccctgcgc gtaattcatg ttcgggttca tgcgcgtctt
     2221 cgcgtcgaga aaccagaccc gcaggatttg cgccgcgcgg tcggcgtagt cccagcggcc
     2281 gctgtagtga taggccaggg ccagggtcgc gacggcctcg ctcatatcgc ccagccgtgt
     2341 gcggtcgaag gcgttggtgg cgcgctccgg gttcacccgt ccgtcgtgat tgacgtaagg
     2401 ctcgccgttc ggctgcgccg gatcgggcca ccagtagggc gcgatgctca tatagtcgtg
     2461 cctgtcgccg ccgggcgtcg cgtgcgtttt atcggtgacg ctccacggtc ccttgcgcag
     2521 ggcggcgtcg gcgtcacgca gcagatcggc ataggccgga tcgccggcga gaccggcccg
     2581 gaccgcgctc atggcgtcgg gccgccataa aaaggtggcc tggcccgcgg cggcatagcc
     2641 gtgcgcgccg tcgcataccg gcgcggacag ggcgggcgcg gcggcgggca gcgcggccat
     2701 gacggcgaaa aaggaaacgg acaaaagatc gggcaggcgc atctgcggct ccggaaaaac
     2761 aacgccgccg ccgcggcgaa agctgaaaaa ggcggccgcc cggcgcgatg tgtctcggtg
     2821 cgcggggcgg cccaggcttg cgggcgggga gacttagaaa ttggcccgaa gcgtgacttc
     2881 ataggtgcgc ccgtcgtaat cga
//
LOCUS       DASVLL010000322         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_505657,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000322 DASVLL010000000
VERSION     DASVLL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505657"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1023)
                     /locus_tag="VF402_05415"
     CDS             complement(277..1023)
                     /locus_tag="VF402_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7799767.1"
                     /translation="MDTMAQRWTRLKPYAQWFGPNDAKPRPAVVLFHGCGGLRPHVVR
                     YARAAAATGVRAVVIDSFAPRGWDRAFAVSFVCTGLVFQGHDRTGDVLATLYGLQQDG
                     RVSDVMLAGFSHGGWTIMDLMTDPLDRPGAAKLADPDPALMARVKALFLVYPYIGFPA
                     RSNTRGWLYRPQTFAALAAKDHLTPLSHARRVLKRLSGGGVPVETAVFDATHAFDEED
                     NKGPIMRFSDVATAGSIAAMQGFIARVFKT"
     gene            complement(1078..1812)
                     /locus_tag="VF402_05420"
     CDS             complement(1078..1812)
                     /locus_tag="VF402_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7799768.1"
                     /translation="MLAPYLTAYGPDDDAPRPAVLLFHACNGVRPHIHAYARQAAALG
                     YRAYVVDSFTPRGWDHQAALALVCSGLALHGYERSGDVLAALWGVAQRPEVDADRMLA
                     AGWSHGGWAIMDLMTERLTRSGEARLQDPTPEPLRGLRGVFAVYPYLGFPARSNTHDW
                     VHRPRAEIILARQDFLTQHSHAAHILDHLRAGGAPVDYRVMEATHCFDEDSALHFGPL
                     MAYDRASHAATEAAFGAFLNTCLGQN"
     gene            1961..>2225
                     /locus_tag="VF402_05425"
     CDS             1961..>2225
                     /locus_tag="VF402_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7799769.1"
                     /translation="MSQPLRHSDHPIQPQFLERWSPRAFSDRPVGEQELMTLFEAARW
                     APSASNLQPWRFIYGLKGEPEFATLLSLLIPFNEGWAKEAAALV"
BASE COUNT          388 a          717 c          762 g          358 t
ORIGIN      
        1 catatgccgg tgtcgtccgc gccgctggcc tcggacagcg atatgtccgg gatgaagatg
       61 taaatctctg cccttccccc tctcccctcc acgaggggag aggacgagag ccgagccgtc
      121 aggcgatggc gatcgggtga ggggctgtca gccggttgag ctcagtcggc gggcggcccc
      181 tcacccgagg atcggcggct tcgccttgct cctcgtcctc tccccattgt catggggaga
      241 gggggaagat tgagacttcc tcatggaggg gcggcttcag gtcttgaaca cccgcgcgat
      301 aaagccctgc atggcggcga tggagcctgc cgtcgccacg tcgctgaagc gcatgatcgg
      361 gcctttattg tcctcctcgt cgaaggcgtg ggtggcgtcg aataccgccg tctcgaccgg
      421 aaccccgccg ccgctcagcc gtttcagcac cctgcgcgcg tgcgacagcg gcgtcaggtg
      481 gtccttcgcc gccagcgccg cgaaggtctg cggccgataa agccagccgc gcgtattgga
      541 gcgcgccgga aaaccgatat agggatagac gaggaacaga gccttgaccc gcgccatcag
      601 cgccgggtcg ggatcggcca gtttggcggc gccgggccgg tcgagcggat cggtcatcag
      661 gtccatgatc gtccagccgc cgtggctgaa tccggccagc atcacgtcgg aaacgcgccc
      721 gtcctgttgc aggccgtaca gcgtcgccag cacgtcgccc gtgcggtcat ggccctgaaa
      781 gacaaggccg gtgcagacga aggacacggc gaaggcgcgg tcccagcctc tcggggcgaa
      841 cgagtcgatc accaccgcgc gcacgcccgt ggcggcggcg gccctggcgt agcggacgac
      901 gtgcggacgc aggccgccgc aaccgtggaa cagcaccacg gcggggcgcg gcttggcgtc
      961 gttcgggccg aaccattgcg cgtaaggctt cagccgcgtc catctctgcg ccatcgtgtc
     1021 catgggcaaa ggcttaacgc ggatcggcgt ccagtgcaag ataaatttga acgatgttca
     1081 attctggccg aggcacgtgt tcagaaaggc gccgaacgcc gcctcggtcg cggcgtggct
     1141 ggcgcggtca taggccatga gcgggccgaa atgcagggcg gaatcctcat cgaagcaatg
     1201 ggtcgcctcc atgacgcggt aatcgacggg cgcgcccccg gcgcgcaggt ggtcgagaat
     1261 atgcgcggcg tggctgtgct gggtcaggaa gtcctgccgc gccagaatga tctcggcgcg
     1321 cgggcggtgg acccagtcgt gcgtgttcga gcgcgccgga aagccgagat agggatagac
     1381 ggcgaaaacg ccgcgcaggc cgcgcagggg ttccggcgtc gggtcttgca gccgcgcctc
     1441 gccggagcgc gtcagccgtt cggtcatcag gtccatgatc gcccagccgc cgtggctcca
     1501 accggcggcg agcatgcggt ccgcgtcgac ctccgggcgc tgcgccacgc cccataaggc
     1561 cgccagcacg tcgccggaac gctcatagcc gtgcagggcc agccccgaac agaccagagc
     1621 cagcgccgcc tgatggtccc aaccgcgcgg cgtgaacgaa tcgaccacat aggcgcgata
     1681 gccgagcgcg gcggcctggc gcgcataggc atggatatgc gggcgcacgc cgttgcaggc
     1741 gtggaacaaa agcacggcgg gacgcggcgc gtcgtcgtcg gggccatagg cggtgaggta
     1801 gggcgccagc atggaaaatc tgtgagccag cgtatcgagc aagccgttcg ccccttgact
     1861 ttaaatgtaa cggacacaat tttagttacg gaacgcggcc cgcctctctc ctgtcaatca
     1921 gaattcgcgg gccatgacca gttttattaa gggtatttgc atgtcacaac cgcttcgcca
     1981 ttccgatcat ccgattcagc cgcaatttct ggagcgctgg tcgccgcgcg ccttctccga
     2041 tcggccggtc ggcgaacagg agttgatgac cctgttcgag gccgcgcgct gggcgccgtc
     2101 ggcgtcgaac ctgcaaccgt ggcggttcat ctacggcctg aagggcgaac cggaattcgc
     2161 cacgctgctg agcctgctga tcccgttcaa cgaaggctgg gccaaggagg ccgccgcgct
     2221 ggtct
//
LOCUS       DASVLL010000323         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_505699,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000323 DASVLL010000000
VERSION     DASVLL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505699"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1318)
                     /gene="aroB"
                     /locus_tag="VF402_05430"
     CDS             complement(164..1318)
                     /gene="aroB"
                     /locus_tag="VF402_05430"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7799770.1"
                     /translation="MTDPRRIPVSGEGFKPYDVIVGQGLLQEAERWIGPFLRNKRVVM
                     VSDSNVAPLHADAILKQFEAAGHRTHLITVPAGETSKSFEGLKFVIDAMIECGLDRKD
                     VVIALGGGVIGDLTGFACAVYMRGVDFIQIPTTVLAQVDSSVGGKTAIDHERGKNLIG
                     AFWQPRLVLCDLDVLATLPAREIRCGYAEIIKYGLLGDRAFFEWLEANDDKVLALEPQ
                     ALQHAIARSVEMKADIVAQDEREGGVRALLNLGHTFGHALEAEVGFGDALLHGEAVAI
                     GMCQAFRYSALSGECTEADEARAVKAIHHAGLPTKMSDIRWRDGRNAPFDACRLIEHM
                     GHDKKAEDGHLTFVLAGGIGRAFVAKKVDRAAILDFLVLDGAIISEDAQC"
     gene            complement(1315..1890)
                     /locus_tag="VF402_05435"
     CDS             complement(1315..1890)
                     /locus_tag="VF402_05435"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7799771.1"
                     /translation="MTAVPEPASSGRPVFALPRTVALVGLMGVGKTTIGRRLAEHFHL
                     PFVDADEEIERAAGQSIPDIFAQHGEAYFRDGEQRVIARLLDGPVQILATGGGAMTHE
                     KTRENLKDRAVTVWLRTDLKVLARRVANRPHRPLLKDRDPMEVLKEHVAKRYPFYEQA
                     DIAVDTGDHPHGRALDLVLDALYNHIQRTSS"
     gene            1984..>2164
                     /locus_tag="VF402_05440"
     CDS             1984..>2164
                     /locus_tag="VF402_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX7799772.1"
                     /translation="MKFDPVGQFLQMLSVERNAARPTLDAYGRDLADLCETLGVDARG
                     LLQLGEADIARYFEAL"
BASE COUNT          355 a          692 c          740 g          377 t
ORIGIN      
        1 caggatgggg atcagagtcg caaaagttgc gatcagcacg gctgatctcc acattccgaa
       61 agctgtgcga cagcttgagg caaggggctt ttgtttgcgc taacgcttcg ctccttgagc
      121 acggcgcccg ctatgcggcg attgagctgc aaaaatgtgg cgattagcac tgcgcgtcct
      181 cgctgatgat ggcgccatcc agcaccagga agtcgagaat ggcggcgcgg tccaccttct
      241 tcgccacaaa ggcgcggcct ataccgccgg ccagcacgaa ggtcaggtgg ccgtcctccg
      301 ccttcttgtc atggcccata tgctcaatga ggcggcaggc gtcgaacggc gcattgcgcc
      361 cgtcccgcca gcggatatca ctcatcttcg tcggcagacc ggcgtgatga atagccttga
      421 ccgcgcgcgc ctcgtccgcc tcggtgcatt cgccgctcag ggcgctgtag cggaaggcct
      481 ggcacatgcc gatggcgacc gcctcgccgt gcagcagcgc gtccccgaag ccgacctcgg
      541 cctccagcgc gtgaccgaag gtgtggccga gattgagcag ggcgcgcacg ccgccttcgc
      601 gctcgtcctg cgccacgatg tcggccttca tctcgacgct gcgggcgatg gcgtgttgca
      661 gcgcctgcgg ctccagcgcc agcaccttgt cgtcgttggc ctccagccac tcgaagaagg
      721 cgcggtcgcc cagaaggccg tacttgatga tctcggcgta gccgcagcga atctcgcgcg
      781 cgggcagggt ggccagcacg tcgaggtcgc acagcaccag acgcggctgc cagaaggcgc
      841 cgatcaggtt cttgccgcgc tcatggtcga tggcggtctt gccgccgacc gaggaatcga
      901 cctgcgccag cacggtggtc ggaatctgga tgaagtcgac gccgcgcata tagaccgcgc
      961 aggcgaagcc cgtcaggtcg ccgatcacgc cgccgcccag cgcgatcacc acgtccttgc
     1021 ggtcgaggcc gcattcgatc atcgcgtcga tgacgaattt cagcccctcg aacgatttgg
     1081 aggtttcgcc cgccgggacg gtgatcaggt gcgtcctgtg accggcggcc tcgaactgtt
     1141 tgaggatcgc gtcggcgtgc agcggcgcga cgttgctgtc gctgaccatg accacgcgct
     1201 tgttgcgcag gaaaggcccg atccagcgct cggcctcctg caacaggccc tgaccgacga
     1261 tcacgtcata gggtttgaag ccttcgccgg aaacgggaat gcggcgggga tcggtcatga
     1321 cgaggtccgt tgaatgtggt tgtacagcgc gtccagcacc agatcgagcg cgcgcccgtg
     1381 cggatgatcg ccggtgtcga cggcgatgtc ggcctgttcg tagaagggat agcgtttggc
     1441 gacgtgctct ttcagcacct ccatcgggtc gcggtccttc agcagcgggc gatgcggacg
     1501 gttggcgacg cggcgggcca gcaccttcag gtcggtgcgc agccagacgg tgacggcgcg
     1561 gtccttcagg ttctcgcgcg tcttttcgtg cgtcatggcg ccgccgccgg tggcgagaat
     1621 ctgcaccggc ccgtccagca ggcgcgcgat cacgcgctgc tcgccgtcgc ggaaataggc
     1681 ctcgccgtgc tgggcgaaga tgtcggggat gctctggccc gccgcgcgct cgatctcctc
     1741 gtcggcgtcg acgaagggca ggtggaaatg ctcggccaga cggcggccga tggtggtctt
     1801 gccgaccccc atcaggccga ccagcgcgac ggtcctgggc agggcgaaga cagggcggcc
     1861 ggaggacgcg ggttcgggaa cggcggtcat gagcgggacg cgcggcccct cgacagagtc
     1921 ggaaaaacgg ttttcatccc atgactttac acgatgttag gggcgcaagg ccagtatgcg
     1981 cttatgaaat tcgatccggt cggccagttc ctgcaaatgc tcagcgtcga gcgcaacgcc
     2041 gcgcggccga cgctggacgc ctacgggcgc gatctggcgg acctgtgcga gaccctgggc
     2101 gtcgatgcgc gcggtttgct gcaactgggc gaggccgaca tcgcgcgcta tttcgaggcg
     2161 ctgg
//
LOCUS       DASVLL010000324         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_396575,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000324 DASVLL010000000
VERSION     DASVLL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396575"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VF402_05445"
     CDS             <1..1446
                     /locus_tag="VF402_05445"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX7799773.1"
                     /translation="HHAILRNYPLMGHFRYIFEYIRPEIRQYFIEDERDPLPFSRDQR
                     AIAYQRAKNASDVRPFGTVLNPDGIGYGWISHSIMPAEIKNADFRIDIGGVQCGKPYR
                     ASILNISGMSFGALGGHAIQALSTGAKLGDFALDTGEGSISPYHRQGGGDLVWQVASG
                     YFGCRTKEGRFDPDLFAATAQSDQVKMIEIKLSQGAKPGHGGVLPKAKITPEIAATRL
                     IGMDRDCVSPARHSAFSTPVEMMRFLQQLRDLSGGKPVGFKLCVGHRFEILAMIKAML
                     VTEIVPDFIVVDGAEGGTGAAPMELSDHVGLPLVEGLSFMHHALIGAGLRDDLKLGAS
                     GKMVSAFDFCRIHALGADYVNAARAFMFSLGCVQARACHTNRCPTGVTTQDKGRQRAL
                     VIEDKAPRVANFHRNTLGAIAQVIGAAGLTHPEQLRPWHIHIRNTVGQIVRGDAAYPA
                     VAHGALLSGGADERLTREWDRADPHSFAPKF"
     gene            complement(1480..>2289)
                     /locus_tag="VF402_05450"
     CDS             complement(1480..>2289)
                     /locus_tag="VF402_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7799774.1"
                     /translation="AVAYRMLGSHAEAEDAVQEAWLRLERAGGDGVDNPAGWLTTVVG
                     RICLDMLRARLRRREDPIDGEAGETQHADPAAGVDEQAALADSVGLALLVVLERLTPA
                     ERLAFVLHDMFDVPFAEIGRIVERSPVAARQLASRARRRVQGLPDAPSADYERRKGVV
                     AAFLAASRNGDFSGLLAVLDPDVVFHADADAMRLGALADMRGAEAVATFFDGKAATAR
                     LALIDGALGLAVVLGGALRVVVRVTVEDERIVAIESIADPARLAAFEVEYL"
BASE COUNT          379 a          827 c          756 g          327 t
ORIGIN      
        1 catcacgcca tcttgcgcaa ctatccgctg atggggcatt tccgctatat tttcgaatat
       61 atacgcccgg aaatccgcca gtatttcatc gaggacgagc gcgatccgct gcccttttcg
      121 cgcgaccagc gcgccatcgc ctatcagcgc gccaagaacg cttcggacgt gcgccccttc
      181 ggcacggtgc tcaacccgga cggcatcggc tacggctgga tttcgcactc catcatgccc
      241 gccgagatca agaacgccga cttccgcatc gacatcggcg gcgttcagtg cggcaagccg
      301 taccgcgcct cgatcctgaa catttccggc atgagcttcg gcgcgctcgg cggccacgcc
      361 atacaggcgc tcagcaccgg cgccaagctg ggcgatttcg cgctggacac cggcgaaggc
      421 tcgatcagcc cctatcaccg tcagggcggc ggcgatctgg tctggcaggt ggccagcggc
      481 tatttcggct gccggacgaa ggagggccgc ttcgacccgg acctgttcgc cgcgaccgcg
      541 cagtccgatc aggtgaagat gatcgagatc aagttgagcc agggcgccaa gcccggccac
      601 ggcggcgtcc tgcccaaggc caagatcacg cccgaaatcg ccgccacgcg cctgatcggc
      661 atggacaggg attgcgtctc cccggcgcgt cattcggcct tttccacccc ggtcgagatg
      721 atgcggttcc tgcaacagtt acgcgacctg tccggcggca agccggtcgg cttcaagctg
      781 tgcgtcggcc accgcttcga gattctggcc atgatcaagg ccatgctggt caccgagatc
      841 gtccccgact tcatcgtggt cgacggcgcc gaaggcggca ccggcgcggc gccgatggag
      901 ttgtccgacc atgtcggcct gccgctggtc gaggggctga gcttcatgca ccacgcgctg
      961 atcggggcgg gcctgcgcga cgacctgaag ctgggcgcgt cgggcaagat ggtgtcggcc
     1021 ttcgacttct gccgcatcca cgcgcttgga gcggattacg tcaacgccgc gcgcgccttc
     1081 atgttctcgc tcggctgcgt gcaggcgcgc gcctgccaca ccaaccggtg cccgaccggc
     1141 gtgaccacgc aggacaaggg ccgccagcgc gctttggtga tcgaggacaa ggccccgcgc
     1201 gtcgccaact tccaccgcaa cacgctgggc gccatcgcgc aggtgatcgg cgcggcgggc
     1261 ctcacgcacc ccgaacaact gcgcccgtgg cacatccata tccgcaacac ggtcggccag
     1321 atcgtgcgcg gcgacgccgc ctaccccgcc gtggcgcacg gcgccctgct cagcggcggc
     1381 gccgacgaac gcctgacccg cgaatgggac cgcgccgacc cgcacagctt cgcgccgaag
     1441 ttctaggccg ccccggccct caggcgccgc caccaggttt cacagatatt ccacctcgaa
     1501 ggccgcaaga cgggcgggat cggcgatcga ttcgatagcg acgatccgct cgtcttcgac
     1561 cgtgacgcgc acgacgaccc gaagcgcgcc gccgaggacg acggcaagac cgagggcgcc
     1621 gtcgatgaga gcgagccgcg cagtggccgc cttgccgtcg aagaacgtcg ccacggcctc
     1681 cgcgccacgc atgtcggcca gcgcgccgag ccgcatcgca tcggcgtccg catggaaaac
     1741 gacatcggga tccagcacgg cgagcaggcc ggagaaatcg ccgttgcgcg aggcggcgag
     1801 gaaggcggcg accacgccct tgcgccgctc gtaatcggcg gacggcgcat ccggcaggcc
     1861 ctgcacgcgg cggcgggcgc ggctcgcgag ttggcgggcg gcgacgggcg agcgctcgac
     1921 aatgcgaccg atctccgcga agggcacgtc gaacatgtcg tgcagcacga aggcgaggcg
     1981 ctcggccggc gtcagccgct ccagcacgac gaggagcgcg aggccgacgg aatcggcaag
     2041 cgccgcctgt tcatccaccc cggcggccgg atcggcgtgt tgcgtctcgc ccgcctcgcc
     2101 gtcgatcggg tcttcgcggc ggcgcaggcg cgcgcgcagc atgtcgaggc agatgcggcc
     2161 gaccacggtg gtgagccaac cggcgggatt gtcgacgccg tccccgcccg cgcgctccag
     2221 ccgcagccaa gcctcctgca ccgcatcctc ggcctcggcg tgcgagccca gcatgcgata
     2281 ggccacggc
//
LOCUS       DASVLL010000325         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_505869,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000325 DASVLL010000000
VERSION     DASVLL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505869"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VF402_05455"
     CDS             complement(<1..764)
                     /locus_tag="VF402_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX7799775.1"
                     /translation="MPDQSASPVPRVAFGQGLLKDGWWFAALSGDLVAGRLQRYVILG
                     EPVLLARDLDGKAYAIRDICPHRAAPLSAGEMVRDGRGGACVVQCPYHGWTFDTSGTC
                     KSVPSLTSDQPMEVERIKVRDYAIREQQGMVWIWIAADPRRPAEPDHEPPTLPGVVGG
                     QNDKIRPLIVERMDFDSHIDHAVVGLMDPAHGPYVHQQWWWRSSKKQLEKSKAFEPRE
                     FGFAMIRHRPSSNSRAYKILGGAPATEITFRLPGIR"
     gene            complement(895..2004)
                     /gene="hisC"
                     /locus_tag="VF402_05460"
     CDS             complement(895..2004)
                     /gene="hisC"
                     /locus_tag="VF402_05460"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7799776.1"
                     /translation="MSDIMDKTAPAPKAGILDIAAYVGGKSKIDGVAEPIKLSSNENA
                     LGCSAKAIEAFEAAKARLFRYPDGHANALRDVVAAYHGLAPERLVFGNGSDEVFAVLN
                     QAWLEPGDNIVTGEHGFLAYRISALACQAQVKLAPEPDQRVEIDALLDLVDERTKIVY
                     ISNPANPTGTWNTPEELAELRARLAPHILLVIDEAYAEFADEPTWASAFGMARASDNV
                     IVTRTFSKIHGLAGLRIGFGYCPLPVIEALERIRLPFNISLPAQAAAIAALQDAEHIE
                     ASRRHVLNWRPRLVQEIRAMGLDVRNSAGNFVLIHFRDGDEAARANAFLMSKGLIVRH
                     VANYGLPQALRVTVGKDEENRAFLDALGEFARTLA"
BASE COUNT          384 a          690 c          727 g          357 t
ORIGIN      
        1 tagcggatgc cgggcaggcg gaaggtgatc tcggtcgcgg gcgcgccgcc gaggatcttg
       61 taggcgcggc tgttggacga cgggcggtgg cggatcatgg cgaagccgaa ctcgcgcggc
      121 tcgaacgcct tggatttctc aagctgcttc ttggacgagc gccaccacca ttgctgatgc
      181 acgtaaggcc cgtgcgccgg gtccatcagg ccgacgaccg cgtggtcgat atggctgtcg
      241 aaatccatgc gctcaacgat cagcggcctg attttgtcgt tctggccgcc gaccacgccg
      301 ggcagggtcg gcggttcgtg gtccggctcg gccgggcggc gcggatcggc ggcgatccag
      361 atccagacca tgccctgctg ttcgcggatc gcatagtcgc gcaccttgat gcgctcgacc
      421 tccatcggct ggtccgaggt cagggacggc acgcttttgc acgtcccgga cgtgtcgaag
      481 gtccagccgt gatacgggca ctgcacgacg caggcgccgc ctctgccgtc gcgcaccatc
      541 tcgcccgccg acaagggcgc ggcgcggtgc gggcagatgt cgcggatcgc atacgccttg
      601 ccgtcgaggt cgcgcgccag cagcaccggc tcgccgagga tgacatagcg ttgcagcctg
      661 cccgcgacca gatcgccgga gagcgcggcg aaccaccagc cgtctttcag cagcccctga
      721 ccgaaggcga cgcggggcac gggcgatgcg gattgatcgg gcataacagc tctttgggcg
      781 ttgaaggaaa cgccaatata aggggatcga cgcaaaacgc caacgccgcg catcatgcga
      841 ccgggtgaag cagggggacc gcacgagcgc agcgagatgt ggtggtgggg tttcttacgc
      901 caaggtccgc gcaaattccc ccagcgcgtc gagaaacgcg cggttctcct cgtccttgcc
      961 gaccgtgacg cgcagggcct gcggcaggcc gtaattggcg acatgacgca cgatcagccc
     1021 cttcgacatc agaaacgcat tggcgcgcgc cgcctcatcc ccgtcgcgga aatggatcag
     1081 cacgaaattg cccgccgaat tgcgcacgtc caggcccatg gcgcggattt cctgcaccag
     1141 acgcggccgc cagttcagca catgccggcg cgaggcttcg atatgttcgg cgtcctgcaa
     1201 ggcggcgatg gcggcggcct gcgcgggcag gctgatgttg aacggcagcc ggatgcgctc
     1261 cagcgcctcg atcaccggca gcgggcaata gccgaagccg atgcgcaggc ccgccagacc
     1321 gtgaatcttc gaaaaggtgc gcgtgacgat gacattgtcc gacgcgcgcg ccatgccgaa
     1381 agcgctggcc caggtcggct cgtcggcgaa ttcggcatag gcttcgtcga tcaccagcag
     1441 gatgtgcggc gccagacgcg cgcgcagctc ggccagttcc tccggcgtgt tccacgtgcc
     1501 ggtcggattg gccgggttgg agatgtagac gatcttggtg cgctcgtcga cgaggtccag
     1561 cagggcgtcg atctcgacgc gctgatccgg ctccggcgcc agcttcacct gcgcctgaca
     1621 ggccagagcc gagatgcggt aggccagaaa gccatgctcg ccggtgacga tattgtcgcc
     1681 cggctccagc cacgcctgat tgagaacggc gaacacctcg tccgagccgt tgccgaacac
     1741 cagccgctcc ggcgccagcc cgtgataggc ggcgaccaca tcgcgcaagg cgttggcgtg
     1801 gccgtcggga tagcggaaca gcctggcctt cgccgcctcg aaggcttcga tcgctttggc
     1861 cgagcaaccc agcgcgttct cgttggacga cagcttgatc ggctcggcca cgccgtcgat
     1921 cttcgacttg ccgccgacat aggcggcgat atcgagaatg cccgctttgg gggccggggc
     1981 ggtcttgtcc attatgtcac tcatgggggt cgctcatggg ggctcgcttc gaaactgcgg
     2041 gtttatagcc acgaaaggcg cgaaaagaca cgaaaatcag gtctaatctg tatgaaccac
     2101 ggaaaacagg gacatgcgtt ccgcacgcaa cgccatgatt acgtgggagg cgttcgca
//
LOCUS       DASVLL010000326         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_342621,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000326 DASVLL010000000
VERSION     DASVLL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342621"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VF402_05465"
     CDS             <1..1513
                     /locus_tag="VF402_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX7799777.1"
                     /translation="TAETYARVVRSAVDTHMNMLRVWGGGVYEDERFYDLCDENGVLV
                     WQDFMFACSMYPNDPALLDSIRLEAEDNVRRLRKHPCLAIWVGNNEIDTAWQNDVENG
                     GWGWKQLYTQAQRDEMWAAYKAIFHEILPQTVAKLDTGRFYWPSSPLAAWDGKNVRHA
                     DLGAAKQSGDIHYWDVWWGQKPFEDYRTHIGRFMSEYGFQSFPELRTVAAYAKPEDYD
                     IFSDVMQAHQRSSIGNGTIKNYMQRDYKVPRDFAHFLYVGQVLQAEGIKMAMEAHRAR
                     RDYCMGSLFWQINDCWPVASWSSIDYYGRWKAQQFYARKSFAPILVSAWRDGDQISVH
                     VVSDELKDRRADLLIEVVDFYGKVLRARRQPLTLKANAAATPYKASVYELLSGFLPAA
                     VLVRARLIAGRATLAEDRLYVRPVKDLDLPDAKVAAKARALPGGDIAIALTADALAKN
                     LYLSFDAADGAFSDNYFDLLPGETVVVTFKPAKPMTAAQAESGLKLLHMAQAT"
     gene            complement(1517..>2123)
                     /locus_tag="VF402_05470"
     CDS             complement(1517..>2123)
                     /locus_tag="VF402_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799778.1"
                     /translation="ADRAVVLAPLAAAAHDTGADIVVGVYDPARPGDLALLLRHGGGW
                     AQYDKRRLVPFLETAYTPGRVSGFIGGGRAMEICKDMDFPALIRADAARGVRLAAVPA
                     GDFGADAWLHARMAMLRGVENGFALVRSAHHGLLSVSDDRGRTVALRAAAPQGMSAVV
                     AAVPLGSGPTLYTRLGDLFAWASVAATLIVAGLALRRGRAD"
BASE COUNT          381 a          721 c          702 g          319 t
ORIGIN      
        1 gaccgcggaa acctatgcgc gcgtggtcag atccgccgtc gacacgcaca tgaacatgct
       61 gcgcgtctgg ggcggcggcg tctatgagga cgagcgcttc tacgacctgt gcgacgagaa
      121 cggcgttctg gtctggcagg acttcatgtt cgcctgctcg atgtacccga acgatccggc
      181 gctgctggac agcatacgcc tcgaagccga ggacaatgtc aggcgcctgc gcaaacaccc
      241 ctgcctcgcc atctgggtcg gcaataacga gatcgacacg gcgtggcaaa atgacgtcga
      301 aaacggcggc tggggctgga aacagcttta tacgcaggcg cagcgcgacg agatgtgggc
      361 ggcctataag gcgatcttcc acgagatatt gccgcagacc gtggcgaagc tcgatacggg
      421 ccggttctac tggccgtcct cgccgctggc cgcgtgggac ggaaagaacg tccggcacgc
      481 cgacctcggc gcggcgaagc aatcgggcga catccattac tgggacgtat ggtgggggca
      541 aaagccgttc gaggattacc gcacccatat cggccgcttc atgagcgagt acggctttca
      601 gtccttcccg gaactgcgca ctgtcgccgc ctacgccaaa ccggaggatt acgacatctt
      661 ctcggacgtg atgcaggcgc atcaacgctc gtcgatcggc aacggcacga tcaagaacta
      721 tatgcagcgc gactataagg ttcccaggga cttcgcgcac ttcctctatg tcggccaggt
      781 cttgcaggcc gaggggatca agatggcgat ggaggcgcac cgcgcgcgcc gcgactattg
      841 catggggtcg ctgttctggc agatcaacga ttgctggccg gtcgcctcgt ggagcagcat
      901 cgactattac ggccgctgga aggcgcagca attctatgcg cgcaaatcct tcgcgccgat
      961 cctcgtctcg gcgtggcgcg acggcgatca gatcagcgtg catgtcgtca gcgacgaatt
     1021 gaaggaccgc cgcgccgacc ttctgatcga ggtggtggac ttttacggca aggttctgcg
     1081 cgcgcggcgc cagccgctga cgctcaaggc caacgccgcc gcgacgccgt acaaggcgtc
     1141 ggtctatgag ctgttgtccg gtttcctgcc cgccgccgtg ctggtgcgcg cgcggctgat
     1201 cgccggtcgg gcgacgctgg ccgaggaccg gctctatgtc cgcccggtca aggatctgga
     1261 cctgccggac gcgaaggtgg ccgcgaaggc gagggcgctg cccggcggcg acatcgccat
     1321 cgcgctgacc gccgacgcgc tggccaagaa cctctatctc agcttcgacg cggcggacgg
     1381 cgcgttcagc gacaactatt tcgacctgct gcccggcgaa accgtcgtcg tgaccttcaa
     1441 acccgccaaa cccatgaccg ccgcgcaagc cgaaagtggt ctcaaactgc tgcacatggc
     1501 gcaggcgacg tgaccttcag tccgcgcgcc cgcgccgcag ggccagcccg gcgacgatca
     1561 gggtcgcggc cacgctggcc caggcgaaca ggtcgccgag gcgcgtgtac aaggtcgggc
     1621 cggaaccgag cggaaccgcg gcgaccacgg cgctcatgcc ctgaggggcg gccgcgcgca
     1681 gggcgaccgt ccggccccgg tcgtcgctga cgctcagcag gccgtgatgc gccgaacgca
     1741 ccagcgcaaa gccgttctcc accccgcgca gcatcgccat gcgggcgtgc agccaggcgt
     1801 cggcgccgaa gtcgcccgcc ggaaccgccg ccagccgcac gccgcgcgcc gcgtcggcgc
     1861 ggatcagggc cggaaagtcc atgtccttgc agatctccat cgcccgcccg ccaccgataa
     1921 agcctgaaac gcggcccggc gtgtaggcgg tttccagaaa cggcacgagg cgccgcttgt
     1981 cgtactgcgc ccagccgccg ccgtggcgca acagcagggc cagatcgccg ggccgcgccg
     2041 gatcatagac gccgacgacg atatccgcgc ccgtgtcgtg cgccgccgcc gccaacggcg
     2101 ccagcacgac ggcgcggtcg gcc
//
LOCUS       DASVLL010000327         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_235325,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000327 DASVLL010000000
VERSION     DASVLL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235325"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF402_05475"
     CDS             <1..201
                     /locus_tag="VF402_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7799779.1"
                     /translation="AWRGAVDAARRGRTVLLIAHRLATLGLCDWIVVLEQGRVAEEGP
                     HDRLVAWNDRYARLIATGVLRR"
     gene            246..848
                     /locus_tag="VF402_05480"
     CDS             246..848
                     /locus_tag="VF402_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7799780.1"
                     /translation="MSSNTVPPARTKPADVRREELMDAALALFIAQGIAATSMDEIVK
                     AAGVSKGGFYHHFASKDALLLALQDRYVGDFLAVLIAASAARPAVDWRGRLDAMLEAA
                     VRHFFAELPVHDVVFHEYAPVERREMNQNPIVDHMAAFLKAGAEAGAWRAERPKLLAV
                     MLFGAMHGACDEAILSPELIDRDGLIALLQDFFWRALRPD"
     gene            complement(849..>1701)
                     /locus_tag="VF402_05485"
     CDS             complement(849..>1701)
                     /locus_tag="VF402_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEX7799781.1"
                     /translation="PYDDAIVPKSSFALGYARATENVTIADCQVSGGYVVGSVLDGAF
                     KPIEANRDTWRLGRIKCGTESNGGFRNIAISNCVFDSCWGLALETEDGAVLEDIAVSN
                     LTLKNCFSAPIFLRLGRRMRGPKGRPVGSFKRVLIDNVTCDSVSQLPINIAGVAGYPV
                     EDVKISNVFFTQSGGGDAVMAARNPPANEDKYPDPNMFGDLPATGAFIRHARNIELSH
                     IEIAVKTPDARPAFWLQDVDGFDASFLKTPAKAPAFHLDRVTDFRSLGNRALPDKRLD
                     APAPMEF"
BASE COUNT          244 a          541 c          618 g          298 t
ORIGIN      
        1 gcgtggcgcg gcgcggtgga cgcggcgcgc cggggccgca ccgtcttgct gatcgcgcat
       61 cgtctggcga cgctcggcct gtgcgactgg atcgtcgtgc tggagcaggg gcgcgtggcg
      121 gaggaggggc cgcacgaccg cctcgtcgcc tggaacgacc gttatgcgcg cctgatcgcc
      181 acgggcgtcc tgcggcgctg aatgcggggt cttcacattt ggcgtgggaa gacttatgca
      241 cgttcatgtc ctcgaatacc gtccctcccg cccgcaccaa gcccgccgac gtccgccggg
      301 aagagctgat ggacgccgcg ctggcgctgt tcatcgcgca gggcatcgcc gcgaccagca
      361 tggacgagat cgtgaaggcg gcgggcgtgt ccaagggcgg cttctatcac catttcgcct
      421 ccaaggacgc gctgctgctg gccttgcagg accgctacgt cggcgacttc ctcgccgtgc
      481 tgatcgcggc gtcggcggcg cggcccgccg tggactggcg cgggcggctg gacgccatgc
      541 tggaggcggc ggtgcggcac ttcttcgcgg agctgcccgt ccatgacgtc gtgttccacg
      601 aatacgcgcc ggtcgagcgc cgcgagatga accagaaccc gatcgtcgac catatggccg
      661 ccttcctcaa ggcgggcgcc gaggcggggg cgtggcgggc cgagcgcccg aagctgctgg
      721 ccgtgatgct gttcggggcc atgcacggcg cctgcgacga ggcgatcctg tcgccggaac
      781 tgatcgaccg ggacgggctg atcgcgttgt tgcaggactt tttctggcgc gcgctgcggc
      841 cggattgatc agaactccat cggcgccggg gcgtcgaggc gtttgtcggg cagggcgcgg
      901 ttgccgaggc tgcggaagtc cgtcacgcgg tcgaggtgga aggcgggcgc tttcgcgggc
      961 gttttcagga aagaggcgtc gaagccgtcc acgtcctgaa gccagaaggc cgggcgcgcg
     1021 tcgggcgtct tgaccgcgat ctcgatgtgc gacagctcga tattgcgcgc gtggcggatg
     1081 aaggcgcccg tggcgggcag gtcgccgaac atgttggggt cggggtattt gtcctcgttg
     1141 gcgggcggat tgcgcgccgc catgacggcg tcgccgccgc cggactgggt gaaaaagacg
     1201 ttgcttatct tcacgtcctc gaccggatag ccggccacgc ccgcgatgtt gatcggcagt
     1261 tgcgaaacgc tgtcgcaggt cacattgtcg atcagcacgc gcttgaagct gccgaccggc
     1321 cttcccttcg ggccgcgcat ccgccgcccc agccgcagga agatcggcgc gctgaaacag
     1381 tttttcaggg tgaggttgct gacggcgatg tcttcgagga cggcgccgtc ctccgtctcc
     1441 agcgccagac cccagcacga gtcgaacacg cagttgctga tggcgatgtt cctgaagccg
     1501 ccgttcgatt cggtgccgca cttgatccgg cccagccgcc atgtgtcgcg gttggcctcg
     1561 atcggcttga acgcgccgtc cagcaccgag ccgacgacat agccgccgct gacctgacag
     1621 tcggcgatgg tgacgttttc ggtggcgcgc gcatagccga gcgcgaacga ggatttcggc
     1681 acgatggcgt cgtcataggg c
//
LOCUS       DASVLL010000328         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_129172,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000328 DASVLL010000000
VERSION     DASVLL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129172"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VF402_05490"
     CDS             <1..1024
                     /locus_tag="VF402_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7799782.1"
                     /translation="FVIVAAFGAFLIFKLTGLWMLIFGAVVIATVLRALAEPLIRYTP
                     LNPAFAVLSVLVAVLAFLAVTVSLFGLQIADQAHVLATQLPAAWSAIQAKLATLPVGA
                     QIRDQIAQLGHQAGGVVSKLPLIAGNVFSSLANVLVALIAGIVLAIDPARYRDGVVFL
                     LPDRHKDRMRDAMNTAGGALRLWFIGQFISMVLVGTLTGIGLSVVGLPSAVTLGLLSG
                     VAQFVPLVGPVAATVPGLLLAAMSGWQSVVWALIVYTGVSQLESNFITPMVQRRVAEI
                     PVIVTLFAVIGFAGLIGPMGVLFAMPLTVIIYTLIRKLYTGDDAREAHRKGLFLVAFL
                     TRKKDK"
     gene            1079..2503
                     /gene="murC"
                     /locus_tag="VF402_05495"
     CDS             1079..2503
                     /gene="murC"
                     /locus_tag="VF402_05495"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7799783.1"
                     /translation="MAHTKRPTPFDLGPVHFVGIGGIGMSGIAEIMLKIGYAVQGSDA
                     KSSANTERLEKLGAKIFIGHAPEHVTDGVCALVYSTAVKHDNPEMMEARRRRIPLVRR
                     AEMLAELMRLQFSIAVGGTHGKTTTTSMVAALLDAGGLDPTVVNGGIINAYGTNAKVG
                     DGEWIVVEADESDGSFLRLKSTLAIVTNIDAEHLDHWGDFDAVKKGFCDFVENIPFYG
                     FAAVCIDHPEVQALSARVENRRLIPYGASPQAEVRCVNIEFGPDGACFDVIFQPSGAD
                     AATWEKLKLPMTGNHNVSNATAAIAIARELGVSEDAIRKGLAGFGGVKRRFTTTGVTS
                     GGVRVIDDYGHHPVEIAAVLRAARQVSKGKVIAVVQPHRYTRLRDLMTEFATCFNDAD
                     TVIVADVYSAGEQPIPGVDKAHLVEALHRFGHRSVMALAAPAELPAVIAAEAKSGDLV
                     VCLGAGDITQWAYALPGQLEALTA"
     gene            2500..>2783
                     /locus_tag="VF402_05500"
     CDS             2500..>2783
                     /locus_tag="VF402_05500"
                     /inference="COORDINATES: protein motif:HMM:NF040323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEX7799784.1"
                     /translation="MTRWYARPILPVADVTVALDYYIAALGFHEDWRHEEDGRLLVAQ
                     VSREGCELILSCQWPDKAGNAMQFISLDHDALIALRADLEARGVAALDGE"
BASE COUNT          420 a          909 c          962 g          492 t
ORIGIN      
        1 gttcgtgatc gtcgccgcgt tcggcgcctt cctgattttc aagctgaccg ggctgtggat
       61 gctgatcttc ggcgcggtgg tgatcgcgac cgtgctgcgg gcgctggccg agccgctgat
      121 ccgctatacg ccgctcaacc ccgccttcgc cgtgctgagc gtgctggtcg cggtgctggc
      181 cttcctggcg gtgacggtgt cgctgttcgg cttgcagatc gccgatcagg cgcacgtgct
      241 ggccacgcaa ctgcccgccg cctggagcgc gattcaggcg aagctggcga ccctgcccgt
      301 cggcgcgcag attcgcgacc agatcgccca actgggccat caggcgggcg gcgtggtgtc
      361 caagctgccg ctgatcgcgg gcaatgtctt ttcctcactc gccaacgtgc tggtggcgct
      421 gatcgcgggc atcgtgctgg ccatcgatcc ggcgcgctat cgcgacggcg tggtcttcct
      481 gctgcccgac cggcacaagg accggatgcg cgacgccatg aacacggcgg gcggcgcgct
      541 gcggctgtgg ttcatcggcc agttcatctc gatggtgctg gtcggcacgc tgaccggcat
      601 cggcctgtcg gtcgtcgggc tgccctcggc ggtaacgctg ggccttctgt cgggcgtggc
      661 gcagttcgtg ccgctggtcg ggccggtggc ggcgaccgtg ccgggcctgc tgctggcggc
      721 catgtcgggc tggcagagcg tggtgtgggc gctgatcgtc tataccggcg tatcgcagct
      781 tgagagcaat ttcatcaccc cgatggtgca gcggcgcgtc gccgagattc cggtcatcgt
      841 caccctgttc gcggtcatcg gcttcgcggg gctgatcggg ccgatgggcg ttctgttcgc
      901 catgccgctg accgtgatca tctatacgct gatccgcaag ctctacaccg gcgacgacgc
      961 gcgcgaggcc catcgcaagg gcctgttcct ggtcgcgttt ctgacgcgca aaaaggacaa
     1021 gtgacacccg ccgtccgatc cgctaaaccg gcccgacctc atcgcaagcg aacctgatat
     1081 ggcccatacc aagcgtccca cccccttcga cctcggcccg gtgcatttcg tcggcatcgg
     1141 cggcatcggc atgagcggca tcgccgaaat catgctcaag atcggctacg ccgtgcaagg
     1201 ctccgacgcg aagtccagcg ccaataccga gcggctggag aagctgggcg cgaaaatctt
     1261 catcggccat gcgccggagc atgtcacgga cggcgtgtgc gcgctggtct attcgaccgc
     1321 cgtgaagcac gacaacccgg agatgatgga ggcgcgccgc cgccgcatcc ctctggtgcg
     1381 ccgcgccgag atgctggccg agctgatgcg gctgcaattc tccatcgcgg tcggcggcac
     1441 gcacggcaag acgacgacca cctcgatggt ggcggcgctg ctggacgcgg gcgggctgga
     1501 ccccacggtg gtcaatggcg gcatcatcaa cgcctacggc accaacgcca aggtcggcga
     1561 cggcgaatgg atcgtggtcg aggccgacga atcggacggc tcgttcctgc ggctgaaatc
     1621 gaccctggcc atcgtcacca atatcgacgc cgagcatctc gaccactggg gggatttcga
     1681 cgcggtgaag aaggggtttt gcgattttgt cgagaacatc ccgttttacg gtttcgccgc
     1741 cgtgtgcatc gaccatcccg aagtgcaggc cctgtcggcg cgcgtcgaga accgccgcct
     1801 aatcccgtac ggcgcctcgc cgcaggccga ggtgcgctgc gtcaacatcg aattcggtcc
     1861 cgacggcgcc tgtttcgatg tgatcttcca gccgtccggc gcggacgccg cgacgtggga
     1921 gaagctgaaa ctgccgatga ccggcaatca caatgtctcc aacgcgacgg cggcgatcgc
     1981 gatcgcgcgc gagctgggcg tttccgagga cgcgatccgt aaggggctgg ccggtttcgg
     2041 cggcgtcaag cgccgcttca ccacgaccgg ggtgacctcc ggcggcgtgc gcgtgatcga
     2101 cgattacggc caccacccgg tcgagatcgc cgccgtgctg cgcgccgcgc ggcaggtaag
     2161 caagggcaag gtgatcgccg tggtgcagcc gcaccgttac acccgcctgc gcgacctgat
     2221 gaccgagttc gccacctgct tcaacgacgc ggatacggtg atcgtcgccg acgtctattc
     2281 ggcgggcgaa cagccgatcc ccggcgtgga caaggcgcat ctggtcgagg ccctgcaccg
     2341 cttcggccac cgcagcgtca tggcgctggc ggcgcccgcc gaactgccgg cggtgatcgc
     2401 ggcggaggcg aagtccggcg acctcgtcgt ctgcctcggc gcgggcgaca tcacgcaatg
     2461 ggcctacgcc ctgccgggtc agcttgaggc gctgacggca tgacgcgctg gtatgcccgc
     2521 ccgatcctgc ccgtggccga tgtgacggtc gcgctggact attatatcgc ggcgctgggt
     2581 ttccatgagg actggcggca tgaggaggac gggcggttgc tggtcgcgca ggtgagccgc
     2641 gaaggctgtg agttgatcct gtcatgccag tggccggaca aggcgggtaa tgcgatgcag
     2701 ttcatatcgc tggaccatga cgcgctgatc gccttgcgcg ccgatctgga ggcgcgcggc
     2761 gtggcggcgc tggacgggga gtg
//
LOCUS       DASVLL010000329         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_182742,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000329 DASVLL010000000
VERSION     DASVLL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182742"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VF402_05505"
     CDS             <1..1206
                     /locus_tag="VF402_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX7799785.1"
                     /translation="SGHDRFGGILMQEYRPDLARLVGEPQMIFRGTELGFTEGPHLYR
                     RGDWCYLIVAEGGTGWNHAVTLARAKDIHGPYAPCPHGPILTSRGCSGLALQRAGHGD
                     LVQTQDGQWLIAYLCARPEPETRRSVLGRETAIQPVEWTPDGWIRPAPPLETADNPVA
                     PEARGPKPSARRYAFSDAALPPDFQWLRSPQPETLFSLTQRPGHLTLIGRETIGSQFE
                     QSLVARRLTALTFEAVTRLDYEPSHFQQSAGLVCYYNASKFHYLYLSQDDEAGRHLRV
                     MSALPDPCRPEAHSRTYPVGAGPVELRAVCDGRRLRFGFRLAGAADWTDLDEVFDATL
                     LSDEMTLPGDPGFTGAFVGMACQDMSGQARPAHFAWFDYRDFAAGVPAALSDPAPGAA
                     RKQGRARTG"
     gene            1536..>2377
                     /locus_tag="VF402_05510"
     CDS             1536..>2377
                     /locus_tag="VF402_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX7799786.1"
                     /translation="MMRKLLVPMLALLVLGGGAPAPARAADGSVYLTRPDDRLAVTVR
                     AAGDGRADDTAAIQAAIDAAAKSGAGGIVWLPSGRYRITHSLFIRPAVRVFGVGPTRP
                     VLLLADHTPGFDAGVVNMVIFTGDDQYAVGPVPEPVPTALPSTPPVRDANSATFYSAL
                     SNVDFEIGAGNPAVACVRMRVAQHAFISHIDFRLGSGFAGVYQAGNIAEDLHFHGGRY
                     GVVTEKTSPAWQFTLIDSSFDGQSAAAIREHEADLTLINVAIRDTPVGIDIDQGYSDS
                     LWGK"
BASE COUNT          356 a          834 c          806 g          381 t
ORIGIN      
        1 agcggccacg accgcttcgg cggcatcctg atgcaggaat accggcccga tctggcgcgc
       61 ctcgtggggg agccgcagat gatcttccgg ggaacggagc ttggcttcac cgaggggccg
      121 catctttatc gccgcgggga ctggtgctac ctcatcgtcg cggagggcgg gaccggctgg
      181 aaccacgccg tcaccctggc gcgcgccaaa gatattcacg ggccttacgc gccgtgtccc
      241 cacgggccga tcctcaccag ccgcggctgc tccggccttg cgctgcaacg cgccgggcac
      301 ggggatctgg tgcagacgca ggacgggcag tggctgatcg cctatctctg cgcccgcccg
      361 gagccggaaa cgcgccgctc ggtgctggga cgcgaaaccg cgattcagcc ggtggaatgg
      421 acgccggacg gctggatccg cccggcgccg ccgctggaga cggcggacaa tccggtcgcg
      481 ccggaggcga gggggccgaa gccgtccgcg cggcgctacg cattttccga tgcggccctg
      541 ccgcccgact ttcaatggct gcgctcgccg cagccggaaa ccctgttcag cctgacgcag
      601 cggcccggac atctgaccct gatcgggcgg gagacgatcg gcagccagtt cgaacaaagc
      661 ctggtggcgc ggcgcctgac cgcgctgacc ttcgaggccg tcacgcggct ggattacgag
      721 ccgtcgcatt tccagcagtc ggcggggctg gtctgctact acaacgccag caagttccat
      781 tacctctacc tcagtcagga cgacgaggcc ggacggcatc tgcgggtcat gtccgccctg
      841 ccggacccgt gccggccgga ggcgcattcg cgaacctatc cggtcggcgc cgggccggtc
      901 gagctgcgcg ccgtgtgcga cgggcggcgc ctgcggttcg gcttccgcct cgccggggcg
      961 gcggactgga cggacctcga cgaggtgttc gacgccaccc tcctgtcgga cgaaatgacg
     1021 ctgcccggcg atccgggctt caccggcgcc tttgtcggca tggcgtgtca ggacatgtcg
     1081 ggtcaggccc gccccgccca tttcgcatgg ttcgactaca gggacttcgc cgccggcgta
     1141 ccggcggccc tgtccgatcc cgcgccggga gccgcgcgaa aacagggccg ggcgcggacc
     1201 ggctgacgcc agagcgcttt ccgaaaagtg tgacgcactt ttcggattaa aaagcgcgta
     1261 acagcataaa gttagagcgc cgatctgatg caatcagatc ggaaaacgct ctaggcgcag
     1321 gcgtccgccg ctccctcaag gacgcctgcc cgaactggcc cgcctgcatc cggcgggccg
     1381 catgtttccg gacgaagccg ataccccccg agcggcttcg tctggaaaca tgcttttttt
     1441 cttaaagccg ggtccggccc ggcgaactgt tgttccgggc ctgccgcgtc ctgcgcgcgg
     1501 cgcgcccttc atccttagcg gcggagagcc gagacatgat gcgtaaactc cttgtgccga
     1561 tgctggccct gctggttctc ggcggcggcg cgcccgctcc ggcgcgggcg gcggacggtt
     1621 cggtctatct gacccggccc gacgacaggc tggcggtgac ggtccgggcg gcgggcgacg
     1681 gcagggcgga cgacaccgcc gccattcagg ccgcgatcga cgccgccgcg aaatccggcg
     1741 ccgggggcat cgtctggctg ccgtccgggc gctatcgcat cacccatagc ctgttcatcc
     1801 ggccggcggt gcgcgtcttc ggcgtcggcc cgacgcgccc ggtcctgctg ctggcggatc
     1861 atacgccggg cttcgatgcg ggcgtggtca acatggtcat cttcaccggc gacgatcagt
     1921 acgccgtcgg ccccgtgccg gagcccgtgc cgacggccct gccgtcgacg ccgcccgtgc
     1981 gcgacgccaa ttcggccacc ttctattccg cgctctccaa tgtggatttc gagatcggcg
     2041 cgggcaatcc ggcggtggcc tgcgtgcgga tgcgcgtcgc gcagcacgcc ttcataagcc
     2101 atatcgactt ccggctcggc tccggcttcg cgggcgtcta tcaggcgggc aacatcgccg
     2161 aggacctgca tttccacggc ggccgctacg gcgtcgtcac cgaaaagacc tcgcccgcct
     2221 ggcagttcac cctgatcgac tccagtttcg acggccagag cgcggcggcc atccgcgagc
     2281 acgaggccga tctgacgctg ataaatgtcg ccatccgcga cacgccggtc ggcatcgata
     2341 tcgatcaggg ctacagcgac agcctgtggg gcaagga
//
LOCUS       DASVLL010000330         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_74801, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000330 DASVLL010000000
VERSION     DASVLL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74801"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1401)
                     /locus_tag="VF402_05515"
     CDS             complement(85..1401)
                     /locus_tag="VF402_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7799787.1"
                     /translation="MAFSPAPRLLVLLGLSVPAALAVGVYAPDAWPWVPLAALALLAV
                     AVLDAAFAPVGQGVALSVQPPPFLDLGRVCETPLGVTFKGYAPAVLELRLDLDARLAG
                     SDYERRLTRDADGRFKAVLRLRAATRGPADIKAAYLRWRGPLGLVHVQKTLRLDAHIV
                     VTPDLKGVEQDAADLFSLDNIYGVRVQNQLYNGSEYHALREYDASQDHRRIDWRSSSR
                     HMKLYSREYQAERNHHIVLAFDTGRLMGEPLAGLKRLDRAIYAGLLLGYTGLKIGDHV
                     RAFSFGARPQPVGPVLSGTHGFDVLKYQLGALTDTVEESNHTLALADLNAKVARRSLI
                     VLFSDFIDEISADLMIEALNRLAKKHLVLFVAFRDEVVEDLMAHAPDTADDVTRAVFA
                     RRLASQRERVLTQLRRQGVEVLEAPSAQLATAIVNRYLALKRADRL"
BASE COUNT          285 a          559 c          504 g          246 t
ORIGIN      
        1 ccttcgcgcg cctcgcggaa tttctgactc ttcagttgca gcaccggttt cacctcagcc
       61 acggatcacc acctttcccc tgtctcacaa cctgtcggcg cgcttcaacg ccagataccg
      121 gttcacgatc gccgtcgcca gttgcgcgct gggcgcctcc agcacctcga cgccctgccg
      181 ccgcaactgc gtcagcacgc gctcgcgctg gctggccaga cggcgcgcga acacggcgcg
      241 cgtcacgtcg tccgccgtgt cgggcgcgtg cgccatcaga tcctcgacca cctcgtcgcg
      301 gaaggccacg aacagcacca gatgcttttt ggccagccgg ttcaacgcct cgatcatcag
      361 atcggcggaa atctcgtcga tgaaatccga gaacagcacg atcagcgacc gccgcgccac
      421 cttggcgttg agatcggcca gggccagggt gtgattgctc tcctcgaccg tatcggtcag
      481 ggcgcccaac tggtatttca gcacgtcgaa gccgtgcgtc ccggacagca ccggcccgac
      541 cggctggggc cgcgcgccga agctgaaggc gcggacgtga tcgccgatct tcagcccggt
      601 atagccgagc agcaggcccg catagatggc ccggtccagc cgtttcagtc cggccagcgg
      661 ctcgcccatc agccgcccgg tgtcgaaggc cagcacgata tggtggttgc gctcggcctg
      721 atattcgcgc gaatagagct tcatgtgccg cgaggacgag cgccagtcga tgcggcgatg
      781 gtcctgcgag gcgtcgtatt cgcgcaaggc gtggtattcc gacccgttgt aaagctggtt
      841 ctgcacccgc acgccgtaga tgttgtcgag cgaaaacagg tcggccgcgt cctgctcgac
      901 gcccttgagg tcgggcgtga cgacgatgtg cgcgtccagc cgcagcgtct tctgcacatg
      961 cacgaggccc agcggcccgc gccagcgcag gtaagccgcc ttgatgtccg cgggcccgcg
     1021 cgtcgccgcc ctgagccgca gcacggcctt gaatcgcccg tccgcgtcgc gcgtcaggcg
     1081 gcgttcatag tccgaccccg ccagccgcgc gtcgagatcg agccgcagct ccagcacggc
     1141 cggcgcatag cccttgaacg tcacgcccag cggcgtctcg cagacgcggc ccagatcgag
     1201 aaacggcggc ggctgcacgc tcagggccac gccctgcccg accggcgcga aggccgcgtc
     1261 cagcacggcc accgccagca gggccagcgc cgccagcgga acccacggcc aggcgtccgg
     1321 cgcgtaaacg ccgaccgcca gagcggcggg gacgctcagg ccgagcagga ccagaagacg
     1381 cggcgcgggg gaaaaggcca tttagagcgc tttccgaaaa gtgtgacgca cttttcggat
     1441 taaaaagcgc gtaaaagtat aaagttagag cgccgatctg atggcatcag atcggaaaac
     1501 gctttaacgc ggaatctcga cgcgttccag aatgcgttgc agcacccggt cgacgctttc
     1561 gccctcgatc tcggcgacgg gcgacagcag cacg
//
LOCUS       DASVLL010000331         5275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_342848,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000331 DASVLL010000000
VERSION     DASVLL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342848"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VF402_05520"
     CDS             complement(<1..758)
                     /locus_tag="VF402_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEX7799788.1"
                     /translation="MEACPAITLVGETSVSGSLQAMDCHIDAAVHLGYDRLFGPGGAF
                     GLALSALLVIYVALIGYGFMTGRTRLTLAMMSPRIMTMALVLTFATAWPAYHAIFYGL
                     LMGGPDEVAAALLHVNGSAVMHFAQQLDGLFVRFADIARALDATGAVDATGAVATPNG
                     QAVMALPHSMPVQLFWLSGLFLLVSTLGVLILTRLVLYLLLILGPVFIIFALFPQTRG
                     LFNGWLRTSLVFALAPLLTVLGGTAALMLFAPLL"
     gene            complement(758..3172)
                     /locus_tag="VF402_05525"
     CDS             complement(758..3172)
                     /locus_tag="VF402_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HEX7799789.1"
                     /translation="MSDTPARSGFSRLFGKEQRAGDRLPYDRLLDERTLRLRDGSLMQ
                     TIHLEGFAFETADTDEVNHRQIVRAAALRAVGSSRFVLYHHIVRRRVMVDLPAAYDDP
                     VCGLIQHRWRGRLSSRQVFVNDLFLTLVRRNPKGKAGFAESLAGLMGRGGGETAQAAQ
                     TAREHKELQGASEALLAALQPYGPRLLGGYETASGRCNELLELLSCLFNGEMRPVLRP
                     TSDVGHYLPYRRVSFGLEALEQASLGAGKDFSALLSLKDYPPHATPGMCDALLRLPFE
                     MTLTESFAFVDRQIGMERVNLALRRMRAADEEALSLRQGLMNAKDDLATGAVGLGEHH
                     LSVLVRAGSLAALDPAVAQALAALADLGAVAVREDVNLEPAFWAQFPGNEAYIARKAL
                     ISTSAYASFASLHGFPIGQPEGNHWGQAVTVFETSSGTPYYFNFHKADLGNFTVIGPS
                     GSGKTVALNFLTAQAQKFQPRTVLFDKDRGAEIFIRAIGGHYSSLRPGEPSGFNPLRM
                     DDTPGNRAFLRALIGRLAARPEAPLSAEEEAIISEAVDANYEQEPQYRRLRFFRELLS
                     GARRPAHGDLAARLAPWVGDGDHAWLFDNAEDALDMDRRTLGFDMTLLLNDPALRTPC
                     MMYLFQRVEERLDGTPTMLIIDEGWKALDDDAFAAAIRNWMKTLRKRNAILGFGTQSA
                     RDALDSRIASAIIEQTATQIFMPNPRAQPEDYCDGFGLTEHELDLIRAIPAASRAFLV
                     KHGNHSVVARLDLSAMPEILTVLSGREQSVRRLDELRARLGDDPAAWWSELTQTSWPG
                     AAAGAA"
     gene            complement(3177..3461)
                     /locus_tag="VF402_05530"
     CDS             complement(3177..3461)
                     /locus_tag="VF402_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB3"
                     /protein_id="HEX7799790.1"
                     /translation="MADLIRTPVFKALTQPQMFAGVTYSFFIINAVATTEAFLVTRSF
                     WAFAVAGVVHAIGYLACLREPRVFDLWLIRVSRCPRVRNYRFWRCNSYAP"
     gene            complement(3462..3767)
                     /locus_tag="VF402_05535"
     CDS             complement(3462..3767)
                     /locus_tag="VF402_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEX7799791.1"
                     /translation="MKRIQHRLMAAAAALAGFAASAQAQTVDPAGSSPISNALDWIQG
                     TLMGRVATTVAVIAVAAVGFMMLTGRMNWKFGATVIVGCFVLFGAATIVAGIQSVAH"
     gene            complement(3786..4427)
                     /locus_tag="VF402_05540"
     CDS             complement(3786..4427)
                     /locus_tag="VF402_05540"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7799792.1"
                     /translation="MSRMKMTGMLIGAGLWACAAAAGAQVISLGDDGRVRMLCTAWQI
                     PRPVAAPQAGPPAAYAAMIARAAAAYDLAPDLLDTLARRESGYDPAARSAKGAIGLMQ
                     LTPATAKALGVDPRDPQQNILGGAALLRRLLDANDGRIDLALAGYNAGQGAVAKYGGV
                     PPYAETREYVRQNLDQLAAISENAVREAEPEASQAPVNADNYVQTCPSDAKGR"
     gene            4659..>5275
                     /locus_tag="VF402_05545"
     CDS             4659..>5275
                     /locus_tag="VF402_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX7799793.1"
                     /translation="MRHLKTALLGLASSLALAASVHAAPIATLDRNGAWVSVEAYGPN
                     VIHVTIAADKDEALKGPGYGILADHADNAGFRETSDANGDVFASSALTLHVNAAPAPH
                     TPSQAEKYFAPSLAPVGLQVTNARGETILNMTGWEMSPHDVNGEHTYQVGASFTAPAD
                     EHYYGMGQNQESLGPLDLRGRVIDCKHWYDAPAGETVCVPFMVSS"
BASE COUNT          912 a         1858 c         1742 g          763 t
ORIGIN      
        1 tgcaacagcg gcgcgaacag catcagcgcc gccgtgccgc ccagcacggt cagcagcggc
       61 gccagcgcga acaccagcga ggtccgcagc cagccgttaa acaggccgcg cgtctgcggg
      121 aacagcgcga agatgatgaa gaccggcccc agaatcagca gcaggtacag caccagccgc
      181 gtcaggatca gcacgcccag cgtcgagacc agcaggaaca ggcccgacag ccagaacaac
      241 tgcaccggca tggaatgcgg cagggccatg accgcctgcc cattcggcgt cgcgaccgcg
      301 cccgtggcgt cgaccgcgcc cgtggcgtcg agcgcgcgcg cgatgtcggc gaagcgcacg
      361 aacaggccgt ccagttgctg cgcgaaatgc atgaccgcgc tgccgttgac gtgcaaaagc
      421 gccgccgcca cttcgtcggg accgcccatc agcaggccgt agaagatagc gtgataggcg
      481 ggccacgcgg tcgcgaaggt caggaccagc gccatcgtca tgatgcgcgg gctcatcatg
      541 gccagggtca ggcgcgtgcg cccggtcatg aagccgtagc cgatcagcgc gacatagatg
      601 accagcagcg cgctcagcgc cagcccgaac gccccgcccg gcccgaacag ccggtcgtag
      661 ccgagatgca ccgccgcgtc gatgtggcag tccatcgcct gcaacgaccc ggagacgctg
      721 gtctcgccga ccagggtgat ggcgggacag gcttccatca ggcggctccg gcggccgcgc
      781 ccggccacga cgtctgcgtc agctcgctcc accacgcggc cggatcgtcg cccagcctgg
      841 cgcgcagttc gtcgaggcgg cgcacgctct gctcgcggcc cgacagcacg gtcaggatct
      901 ccggcatggc cgacaggtcc agccgcgcca cgaccgaatg gttgccatgt ttgaccagga
      961 acgcgcgcga ggccgccggt atcgcccgga tcaggtccag ctcgtgttcc gtcagcccga
     1021 agccgtcgca atagtcctca ggctgggcgc gcggattggg catgaagatc tgcgtcgcgg
     1081 tctgctcgat gatcgccgac gctatgcgcg aatcgagcgc gtcgcgcgcc gactgggtgc
     1141 cgaagcccag tatggcgttg cgcttgcgca gcgtcttcat ccagttgcgg atggcggcgg
     1201 cgaaggcgtc gtcgtcgagc gccttccagc cctcgtcgat gatcagcatc gtcggcgttc
     1261 cgtccagccg ctcctcgacg cgctggaaca ggtacatcat gcacggcgtg cgcagggccg
     1321 gatcgttgag cagcagcgtc atgtcgaagc cgagcgtgcg ccggtccatg tccagcgcgt
     1381 cctcggcgtt gtcgaacagc caggcgtgat cgccgtcccc gacccacggc gccagccgcg
     1441 cggccagatc gccgtgcgcc gggcggcgcg cgccgctcaa gagttcgcgg aagaagcgca
     1501 ggcggcggta ttgcggctcc tgctcgtaat tggcgtcgac cgcctcggag atgatcgcct
     1561 cctcctcggc gctcagcggc gcctcaggcc gcgcggccag ccgcccgatc agcgcgcgca
     1621 ggaaggcgcg attgcccggc gtgtcgtcca tgcgcagcgg attgaagccc gacggctcgc
     1681 cgggacgcag gctggaataa tggccgccga tggcgcggat gaagatttcg gcgccgcgat
     1741 ccttgtcgaa cagcacggtg cgcggctgga acttctgcgc ctgcgccgtc aggaagttga
     1801 gcgccaccgt cttgccggag ccggacgggc cgatgacggt gaaattgccg aggtccgcct
     1861 tgtggaagtt gaaatagtag ggcgtgccgg acgaggtttc gaacaccgtc accgcctgcc
     1921 cccagtgatt gccctcaggc tggccgatcg gaaagccgtg caggctggcg aaggacgcat
     1981 aggccgaggt cgagatcagc gccttgcgcg cgatataggc ttcgttgccc ggaaactgcg
     2041 cccagaaggc gggctccaga ttgacgtcct cgcgcaccgc caccgcgccg agatcggcca
     2101 gcgccgccag cgcctgcgcc accgccgggt cgagcgcggc cagcgaaccg gcgcgcacca
     2161 gcacgctcag atgatgctcg cccagcccca cggcgcccgt ggccagatcg tccttggcgt
     2221 tcatcagccc ctgacgcagc gacagggcct cctcgtccgc cgcgcgcatc cggcgcaggg
     2281 ccaggttgac gcgctccatg ccgatctggc ggtcgacgaa ggcgaagctc tcggtcaggg
     2341 tcatctcgaa cggcagccgc agcagcgcgt cgcacatgcc gggcgtggcg tgcggcggat
     2401 agtccttgag cgacagcagg gcggaaaaat ccttgcccgc gccgaggctg gcctgctcca
     2461 gcgcctccag cccgaagctg acgcggcgat agggcaggta atggccgacg tccgaggtcg
     2521 ggcgcagcac cggccgcatc tcgccgttga acaggcagga cagaagctcc agcagctcgt
     2581 tgcagcgccc gctggccgtc tcgtacccgc ccagcaggcg cgggccgtag ggctgcaacg
     2641 cggccagcag cgcctcggag gcgccctgca actccttatg ctcgcgcgcg gtctgggcgg
     2701 cctgcgccgt ctcgccgccg ccgcgcccca tcagcccggc caggctttcg gcgaaccccg
     2761 ccttgccctt cggattgcgg cgcacaaggg tcaggaacag gtcgttgacg aacacctgac
     2821 gcgacgacag gcgcccgcgc cacctgtgct ggatcaggcc gcagaccgga tcgtcatagg
     2881 ccgccggcag gtccaccatc acgcggcggc gcacgatatg gtgatagagg acgaagcgcg
     2941 acgagccgac cgcgcgcagc gcggcggcgc gcacgatctg gcggtgattg acctcgtcgg
     3001 tgtccgccgt ctcgaaggcg aagccctcca gatggatcgt ctgcatcagc gagccgtcgc
     3061 gcaggcgcag cgtgcgttcg tccagcagcc ggtcgtaggg caggcggtcg cccgcgcgct
     3121 gctccttgcc gaacaggcgc gaaaaaccgg aacgggcggg ggtgtcggac atggcgttac
     3181 ggcgcatagg agttgcagcg ccagaaccgg tagttgcgca cgcgcgggca gcgcgaaacc
     3241 cggatcagcc acaggtcgaa gacgcgcggc tcacgcagac aggccagata gccgatggcg
     3301 tgaaccaccc ccgcgaccgc gaacgcccag aaggagcgcg tgaccagaaa cgcctcggtc
     3361 gtcgccacgg cgttgatgat gaagaaggaa taggtcacgc ccgcgaacat ctgcggctgg
     3421 gtcagcgcct tgaacaccgg ggtgcggatc aggtcggcca tctaatgcgc gaccgactgg
     3481 atgcccgcga cgatggtggc ggcgccgaac aggacgaagc agccgacgat cacggtggcg
     3541 ccgaatttcc agttcatgcg cccggtcagc atcatgaagc cgaccgccgc gacggcgatc
     3601 accgccaccg tggtcgccac ccgccccatc agcgtgccct gaatccagtc cagcgcgttg
     3661 gaaatcggcg acgaccccgc cggatcgacg gtttgcgcct gcgccgaggc ggcgaatccc
     3721 gccagagcgg cggcggcggc catcagccgg tgctgaatgc gtttcatggt cttaactctc
     3781 cccgatcatc ggccctttgc gtccgacgga catgtttgca catagttatc ggcatttacc
     3841 ggcgcttggc tagcctccgg ttcggcctcc cgcacggcat tttcgctgat cgccgccagt
     3901 tgatcaagat tttgtctgac atattcacgc gtttccgcat agggcggcac gccgccatac
     3961 ttcgccaccg ccccctgacc ggcattatag cccgccagcg ccagatcgat gcgcccgtca
     4021 ttggcgtcca gcaaccgccg caacagcgcc gctccgccga ggatattctg ctgcggatcg
     4081 cgcggatcga cgcccagcgc cttcgccgtg gcgggcgtca actgcatcag gccgatcgcg
     4141 cccttcgccg accgcgccgc cggatcgtag cccgattccc gccgcgccag cgtatcgagc
     4201 aggtccggcg ccagatcgta ggccgccgcc gcccgcgcga tcatcgccgc ataggccgcg
     4261 ggcggccccg cctgcggagc ggcgacgggg cgcggaatct gccacgcggt gcagagcatc
     4321 cgcacgcgcc cgtcgtcgcc caggctgatc acctgcgcgc ccgccgccgc tgcgcaggcc
     4381 cacaggcccg cgccgatcag catccccgtc atcttcatcc tgctcatgga gggcaatgct
     4441 agcccgataa accgaccgat tccaagacgc gaatattgcg cgctttagaa ttcagtcttc
     4501 gccgcctttg cgctcatgcc tgaaatgcgc gggccgggcg atttcacctt tgaaaccgcg
     4561 acgcgaaagt ccccgacttg tacgataaat ggcttgacgc cggatcgcgc gtctctaagt
     4621 tgccgctcaa tgacaacgct atcagggaag aaagtcacat gcgtcacctg aaaaccgccc
     4681 tgctggggct ggcctcgtcg ctcgcgctgg cggcgtccgt ccacgccgcg ccgatcgcca
     4741 cgctggaccg caacggcgcc tgggtcagcg tcgaggctta cggcccgaac gtgatccatg
     4801 tcaccatcgc cgccgacaag gacgaggcgc tcaaaggccc cggctacggc attttagccg
     4861 accacgccga caatgcgggc ttccgcgaga ccagcgacgc caacggcgat gtcttcgcgt
     4921 cgagcgccct caccctgcac gtcaacgccg cgcccgcgcc gcatacgcca tcgcaggcgg
     4981 aaaagtattt cgcgccgtcg ctggcccccg tcggtttgca ggtgacgaac gccaggggcg
     5041 agacgatcct gaacatgacc ggctgggaga tgtcgccgca cgacgtcaac ggcgagcata
     5101 cctatcaggt cggcgcttcg ttcaccgccc ccgccgacga acactattac ggcatgggcc
     5161 agaatcagga atcgctgggg ccgctggacc tgcgcggccg ggtgatcgac tgcaagcact
     5221 ggtacgacgc cccggcgggc gagacggtgt gcgtgccctt catggtgtcg tccaa
//
LOCUS       DASVLL010000332         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_235526,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000332 DASVLL010000000
VERSION     DASVLL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235526"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VF402_05550"
     CDS             <1..1825
                     /locus_tag="VF402_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7799794.1"
                     /translation="RAVKTSARLSDDRLLSTDSFAADGMDALTRALAAVPKSVVSDKT
                     AAPAATPVVMNAAPVPYRLIAGQSKAVAARPYVAPLSPQDAALYRTAFTLIDQGRYDD
                     AEAQLAQVGDKSLMGYAEYHKLLSRGYTSTYDELIAWLNAYGDQPNAMKVWNLAKRKK
                     PDGAPDPAFPSLNGAQALAPGAASDAVALSASVPLSALSGARAPDGDDAAPELDSALT
                     PKSARSAYNNGDLDGAVKLGRQIGDHWVAGLALWRLKRFDQALTEFKFVATDPSRNAW
                     SQSGGAYWAGRCALQLKDQDDAQSWFKIAASFPFTFYGLVAEARLGVTPAVTLAKKGL
                     PPSFNVDSRDALKASLTGDFAWALSDPRAVRLDALVQIGRIDDAQDELQSAIQHAGGA
                     ADRDNWLALAAKTNVPFNQIATSDWLFDASSYPMPDYGPGSGLKVDKALLFAFARKES
                     KFNPSAYSYAGAYGLLQLMPATAALVENDSSFTRRPKKLLDPDVNLKVGQDYINRLQD
                     SRIVSGDLLRTIAAYNAGPRPVKDALDSLGDGSDSLLVMESIPVAQTRQYVEEVAASY
                     WIYRQLMDQDTPTLAMAASDAPVIAVGKDEAAPAPVLTASN"
     gene            complement(1822..>2324)
                     /locus_tag="VF402_05555"
     CDS             complement(1822..>2324)
                     /locus_tag="VF402_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX7799795.1"
                     /translation="LENRMRFPLEVFEAVRAAYPAHKPVGVRVSATDWVEGGWDLDQT
                     VEFAQALKARGVDFIDVSSGGLSPQQQIAAGPGYQVPFAEAVRARTGVTTFAVGLITE
                     PRQAEAIVMEGKADAVALARAMLYDPRWPWRAAAELGATVDAPPQYWRSAPHGVKGLF
                     GPDAIA"
BASE COUNT          373 a          842 c          767 g          342 t
ORIGIN      
        1 cagggccgtg aaaacctcgg cgcggctgtc cgacgaccgg ctgctctcga ccgattcgtt
       61 cgccgccgac ggcatggacg cgctgacgcg cgcgctggcc gccgtgccga aatcggtcgt
      121 ttccgacaag accgccgcgc ccgccgcgac gccggtcgtg atgaacgccg cgccggtgcc
      181 ctaccgcctg atcgcgggcc agagcaaggc cgtcgccgcc aggccctatg tcgcgccgct
      241 gtcgccgcag gacgccgccc tttatcgcac cgccttcacc ctgatcgatc agggccgcta
      301 cgacgacgcc gaggcgcaac tggcgcaggt cggcgacaag agcctgatgg gctacgccga
      361 atatcacaag ctgctcagcc ggggctacac ctcgacctat gacgaactga tcgcgtggct
      421 caacgcctat ggcgaccagc cgaacgcgat gaaggtgtgg aacctggcca agcgcaagaa
      481 gcccgatggc gcgccggacc cggccttccc gtcgctgaac ggcgcgcagg ccctggcgcc
      541 cggcgcggct tcggatgcgg tggcgctgtc ggccagcgtt cccctgtccg ccctgagcgg
      601 cgcgcgcgcg ccggacggcg acgacgcggc cccggagctg gattcggcgc tgacgccgaa
      661 atcggcgcgc tcggcctaca ataacggcga cctcgacggc gcggtgaagc tgggccgcca
      721 gatcggcgat cactgggtgg cgggcctggc cctgtggcgg ctcaagcgct tcgatcaggc
      781 gctgaccgag ttcaaattcg tcgccaccga ccccagccgc aacgcctgga gccagtccgg
      841 cggcgcctac tgggcgggcc gctgcgcctt gcagctcaag gatcaggacg acgcgcagtc
      901 gtggttcaag atcgcggcca gcttcccgtt caccttctac ggcctcgtcg ccgaggcgcg
      961 gctgggcgtg actcccgccg tgacgctggc gaaaaagggc ctgccgccca gcttcaacgt
     1021 cgattcgcgc gacgcgctca aggccagcct gaccggcgac ttcgcttggg cgttgtccga
     1081 tccgcgcgcg gtccgtctcg acgcgctggt gcagatcggc cgcatcgacg acgcgcagga
     1141 cgagctgcaa tcggcgatcc agcacgcggg cggcgcggcg gaccgcgaca actggctggc
     1201 tttggcggcg aagaccaatg tgccgttcaa ccagatcgcg acctcggact ggctgttcga
     1261 cgcctccagc tatccgatgc ccgattatgg ccccggcagc ggcctcaagg tcgacaaggc
     1321 gcttttgttc gccttcgcgc gcaaggaaag caagttcaac ccctccgcct attcctacgc
     1381 cggcgcctac gggcttttac aactgatgcc cgcgacggcg gcgctggtcg agaacgattc
     1441 cagcttcacc cggcggccga agaagctgct cgacccggac gtcaatctca aggtcggtca
     1501 ggactatatc aaccgtttgc aggattcgcg catcgtgtcg ggcgaccttt tgcgcaccat
     1561 cgccgcctat aacgccgggc cgcgcccggt gaaggacgcg ctcgactcgc tgggcgacgg
     1621 ttcggattcg ctgctggtga tggagtcgat cccggtggcg cagacgcggc aatatgtcga
     1681 agaggtcgcg gcctcctact ggatctaccg gcaactgatg gatcaggaca cgccgactct
     1741 ggccatggcc gccagcgacg cgccggtgat cgcggtcggc aaggacgagg cggcgcccgc
     1801 cccggtcctc accgcctcca actaagctat agcgtccggc ccgaacaggc ccttcacgcc
     1861 gtgcggcgcg gaccgccagt attgcggcgg cgcgtccacc gtcgcgccca gctccgccgc
     1921 cgcacgccac ggccagcgcg gatcgtagag catggcgcgc gccagcgcca cggcgtcggc
     1981 cttgccctcc ataacgatag cctccgcctg ccgcggctcg gtgatcaggc cgaccgcgaa
     2041 ggtcgtcacg ccggttcggg cgcgcaccgc ctcggcgaac ggaacctgat agccgggacc
     2101 ggcggcgatc tgctgctgcg gcgacaggcc gccggacgac acgtcgatga agtcgacgcc
     2161 gcgcgccttg agcgcctgcg cgaactctac ggtctgatcg agatcccagc cgccttcgac
     2221 ccagtcggtc gccgagacgc gcacgccgac cggcttgtgc gcaggataag ccgcgcgcac
     2281 cgcctcgaac acctccagcg gaaagcgcat gcggttctcc agcg
//
LOCUS       DASVLL010000333         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_235545,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000333 DASVLL010000000
VERSION     DASVLL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235545"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VF402_05560"
     CDS             complement(<1..864)
                     /locus_tag="VF402_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799796.1"
                     /translation="MSSRATSFFRWELVLLLWLAFFLNQGDRQIFGVTLPLLKADFHL
                     SDTQMGLIATTFSIVFGILVPIAGWAGDFIRRDWVVIASLLIFSLGTLLTGTAAAFLP
                     LLVYRGVATGVGEALYAPAANSLIGSLHDKTRGRALALHQSANYTGVVFGSFLAGWIA
                     DHYGWRWSFLAFGSLGAVLAVVIALRTRGKVTSVRSGSIARDLAVLPEAFMTIVKSPA
                     LIAQVIGFSGLVFVLAGYLTWTPSLLTERFHLSLAQAGFQSVAWHHLLAYAGLLAAGL
                     IGDRFAATFPRI"
     gene            complement(861..1205)
                     /locus_tag="VF402_05565"
     CDS             complement(861..1205)
                     /locus_tag="VF402_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799797.1"
                     /translation="MLRESVTYVIARNETWTGAAATEPVEAGWATEAVFFVRALADPL
                     ADPEGRLPRIRAQISPDGMRWIDEGACAPMPTSVDDLQALRVRHFGNWLRLKADLPDG
                     ARCTVLVTVHLK"
     gene            complement(1205..>1883)
                     /locus_tag="VF402_05570"
     CDS             complement(1205..>1883)
                     /locus_tag="VF402_05570"
                     /inference="COORDINATES: protein motif:HMM:NF042832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6772 family protein"
                     /protein_id="HEX7799798.1"
                     /translation="HWDAGSHGAFDGAYAMKVATKPKKDALSVAIKRLTFRKACPVRL
                     EFYATCKPEATDLSLSDRDLKSIGFLFDLQVGDQTGPNGQRVMPHLRYLNARNGELQQ
                     KWQFKRTAPPVAEIGDSAETVSHFHLADEGWEDLTGGAQKMAYNEIATKVNWHYFRFD
                     FDLETMTALRFQCNDRVFDLSGFDSIRFPAMKNLWCMLNICLFVETAADKRAFLYVDS
                     VCLSGDF"
BASE COUNT          379 a          579 c          632 g          293 t
ORIGIN      
        1 gatgcgcgga aacgtcgcgg cgaagcggtc gccgatcagg cccgcggcca gcagcccggc
       61 ataggccagc aaatggtgcc aagcgaccga ctgaaacccg gcctgagcca gagacaggtg
      121 gaaccgctcg gtcagcaggg acggcgtcca ggtcagatac ccggccagca cgaagaccag
      181 accggaaaag ccgatgacct gagcgatcag ggccgggctt ttgacgatgg tcatgaaggc
      241 ttcgggcagg acggccagat cgcgggcgat cgagccgctg cgcacgctgg tcaccttgcc
      301 gcgcgtgcgc agggcgatca ccacggccag gacggcgccg aggctgccga acgccaggaa
      361 actccagcgc cagccgtaat ggtcggcgat ccatccggcg aggaaggagc cgaacaccac
      421 gcccgtatag ttggcgctct gatgcagggc gagcgcccgg ccgcgcgtct tgtcgtgcaa
      481 ggatccgatc agactgttgg cggcgggggc gtagagcgct tccccgaccc cggtggccac
      541 gccgcggtag acgagcagcg gcagaaacgc cgccgccgtt ccggtcagca atgtccccag
      601 actgaaaatc agcagcgagg cgatcaccac ccagtcgcgg cggatgaaat cgcccgccca
      661 gccggcgatc ggcaccagaa tgccgaaaac gatggaaaag gtggtggcga tcagccccat
      721 ctgtgtgtcg ctgaggtgga aatcggcctt gagcagcggc agggtgacgc cgaaaatctg
      781 gcggtcgccc tgattaagga aaaaggcgag ccacagcaga agaaccagct cccagcggaa
      841 aaaggacgtg gcgcgcgaac tcatttgaga tgcaccgtga ccagcaccgt gcatctggcg
      901 ccgtccggca ggtccgcctt cagtcgcagc cagttgccga agtggcgcac gcgcagcgcc
      961 tggagatcgt cgacgctggt tggcatgggc gcgcaggcgc cttcatcgat ccaacgcatg
     1021 ccgtcaggag aaatctgcgc cctgatccgg gggaggcggc cttcgggatc ggccaaagga
     1081 tcggccagcg cccgcacgaa gaagacggct tcggtggccc atccggcctc gaccggttcg
     1141 gtggccgcgg cgcctgtcca ggtttcgttg cgggcaatga cgtaggtgac gctttcgcgc
     1201 agcatcagaa atctcccgac agacagacgg agtcgacata gaggaaggcg cgcttgtcgg
     1261 ccgccgtttc gacgaacaga caaatgttca gcatgcacca caggtttttc atcgccggaa
     1321 agcggatcga atcgaaaccg ctcaggtcga agacgcggtc attgcactga aagcgcaggg
     1381 cggtcatcgt ttccagatcg aaatcgaagc ggaaatagtg ccagttcacc ttggtggcga
     1441 tctcgttata ggccattttc tgcgccccgc cggtcagatc ctcccagcct tcgtcggcaa
     1501 ggtggaagtg gctgacggtc tcggcgctgt cgccgatctc ggcgacaggc ggcgccgtgc
     1561 gcttgaactg ccacttttgt tgcaactcgc cgtttcgggc gttgagatag cgcaggtggg
     1621 gcatgacgcg ctggccgttc gggcccgtct ggtcgccgac ctgaaggtcg aacaggaaac
     1681 cgatcgattt cagatcgcgg tcggagaggg agagatcggt cgcctccggc ttgcacgtgg
     1741 cgtagaattc cagccgcacc ggacacgcct tgcggaaggt cagccgcttg atggccaccg
     1801 ataacgcgtc cttcttcggc ttggtggcga ccttcatggc gtaggcgccg tcaaacgcgc
     1861 cgtgcgaacc ggcgtcccag tgc
//
LOCUS       DASVLL010000334         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_506265,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000334 DASVLL010000000
VERSION     DASVLL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506265"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF402_05575"
     CDS             complement(<1..276)
                     /locus_tag="VF402_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7799799.1"
                     /translation="MIDADSPKPLLRSEMKRRRSALAAAGLLAGDRMAEAVEAAFDAA
                     GLWPRAGAVIAGYYPIQSEINPFPLMQLFETRGYALALPCLLPQGEGF"
     regulatory      575..728
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            757..1755
                     /locus_tag="VF402_05580"
     CDS             757..1755
                     /locus_tag="VF402_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX7799800.1"
                     /translation="MILTLGLFLASAVAIYLACEVFVNGIEWVGKRLNLGATAVGSVL
                     AAFGTALPESAVTLVAVVFGHTPQQKDIGVGAAMGGPLVLATIAYGVVGLALWLNSRK
                     LGRGTAMVQVEHGRLSRDQLAFLAVFVVKVGLGLVAFAFKPWLGVLFLAAYGVYVWRE
                     ITTAETDAEEEDLEPLKIRPTQPSLFWAGLQSGLALLVIAFASRLFVGQIEHIGEYLH
                     LAPHLIALLLSPVATELPETMNAIIWVRQGKERLALANISGAMMIQATIPSALGIFFT
                     PWRFDHALLAAGVVTALAIIVLFCMFRRGRVDARALAAVAGLYGVFALWLALAPAI"
     gene            1807..2106
                     /locus_tag="VF402_05585"
     CDS             1807..2106
                     /locus_tag="VF402_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7799801.1"
                     /translation="MSEDCKQPPGVFQPVIDRARCEGKADCVVICPYDVFELDLLPPE
                     DRKALSLGARVKGFVHGWKQSFAVRADACRACGLCVKACPEKAIRLQRVSENPPR"
BASE COUNT          354 a          724 c          768 g          393 t
ORIGIN      
        1 gaagccctcg ccctgcggca gcaggcacgg caaggccagc gcgtagccgc gcgtctcgaa
       61 aagctgcatg agcggaaacg ggttgatctc ggactggatc gggtaatagc cggcgatcac
      121 ggcgcccgcg cgcggccaga gcccggcggc gtcgaacgcc gcctcgaccg cctcggccat
      181 gcggtcgccc gccagcagac cggcggcggc cagagccgac cggcggcgtt tcatctcgct
      241 gcgcagcagg ggcttgggcg agtcggcgtc gatcatgcgg cgcggtccgt cagaatttca
      301 agggtggcgg cgccgcgtaa gaaccgtgtc gaaggtttaa atcctctcag acctgacaag
      361 tcaggtggga accggatgat gaggaccacg gtccaaacca gcgccagccc ccgttctaag
      421 tgattaaggt cagaaggata ggtttcgcca cgcgagccgc agcaagaccc gccgtcgtct
      481 ataatagggg cgcaggggcg cgaactcaag acggacgcgc aggccgaggc aaagatttcg
      541 ctttccttgc gcgcggaaat ccggcatagg cgcgtttatc gtcattgggg agtagccgcc
      601 tttgctcgca aaggggccat gtcaacataa taggcgccgc agcgccttgg catgtccata
      661 accggcaaga cctttggtcg tggaagcggt tgtccggccg ggcaagacgc ttgcgcgcgc
      721 catcggttga tcgcttgccc ggcccaagac tgaaacatga tcctgacact tggtcttttt
      781 ctcgcttccg ccgtggcgat ctatctggcg tgcgaggttt tcgtcaacgg catcgaatgg
      841 gtcggcaagc ggctcaatct gggcgccacc gccgtcggct cggtgctggc cgccttcggc
      901 acggcgctgc cggaaagcgc ggtcacgctg gtcgccgtcg tgttcggcca tacgccgcag
      961 caaaaggata tcggcgtagg cgcggcgatg ggcgggccgc tggtgctggc gaccatcgct
     1021 tacggcgtgg tcggtctggc cctgtggctg aacagccgca agctggggcg cggcacggcg
     1081 atggtgcagg tcgagcacgg ccgcctgagc cgcgaccaac tggcgtttct ggctgtcttc
     1141 gtcgtcaagg tcgggctggg gctggtcgcg ttcgcgttca agccgtggct gggcgtgctg
     1201 ttcctggccg cctacggcgt ctatgtctgg cgcgaaatca ccacggcgga aacggatgcg
     1261 gaggaggaag acctcgaacc gctcaagatc cgccctacgc agcccagcct gttctgggcc
     1321 ggtttgcagt cgggtctggc cctgctggtc atcgccttcg cctcgcgcct gttcgtcggc
     1381 cagatcgagc atatcggcga atatctccat ctggcgccgc acctgatcgc gctgctgttg
     1441 tcgccggtgg cgaccgagct gcccgaaacc atgaacgcga tcatctgggt gcggcagggc
     1501 aaggagcggc tggccctggc caacatctcc ggcgcgatga tgatccaggc caccattccc
     1561 tcggcgctgg ggatcttctt cacgccgtgg cgcttcgacc atgcgctgct ggcggcgggc
     1621 gtggtgacgg cgctggccat tatcgtgctg ttctgcatgt tccggcgcgg cagggtcgac
     1681 gcgcgcgcgc tggccgccgt cgcggggctt tacggcgttt tcgccctatg gctggcgctg
     1741 gcgccagcca tataaaatca ggatctgcgg ctggccttcg ccgggctggg ctgatagaca
     1801 accgtcatga gcgaagactg caaacagccg cccggcgtct ttcagccggt catcgaccgc
     1861 gcgcgctgcg agggcaaggc ggactgcgtc gtcatctgcc cctatgacgt gttcgagctg
     1921 gacctgctgc cgccggagga ccgcaaggcc ttatccctcg gcgccagggt caagggcttc
     1981 gtccacggct ggaaacagag cttcgccgtc cgcgccgacg cctgccgcgc ctgcggcctg
     2041 tgcgtcaagg cgtgcccgga aaaggcgatc cggttgcagc gcgtcagtga aaatccgccc
     2101 cgctgagggt ttctcccgcc gcttcctccg ccgccctggc gaccggcgcg tcgcgcgagc
     2161 gcaggggccg cgcctccatc gtcagcaggc acaggaaggc cgcgcccatg gcggcggcgg
     2221 cgccgatgaa caccggctt
//
LOCUS       DASVLL010000335         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_343066,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000335 DASVLL010000000
VERSION     DASVLL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343066"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="manD"
                     /locus_tag="VF402_05590"
     CDS             complement(<1..1089)
                     /gene="manD"
                     /locus_tag="VF402_05590"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEX7799802.1"
                     /translation="MLPKLAPAPSPPSAPARARIVSARVIVTCPGRNFVTLKIETDQG
                     VYGVGDATLNGREKAVAAYLEAHVIPCLIGRDAHGIEDVWQYLYRGAYWRRGPVTMTA
                     IAAVDMALWDIKAKIAGLPLYQLLGGACRDGMMVYGHANGVSIEETVEVAHSYKAQGY
                     KAIRLQCGVPGLSSTYGVSKDRAFYEPADSDLPAENVWDTESYMRVVPKLFAAARDAL
                     GDETHLLHDVHHRLTPIEAGRLGRDLEPYRPFWLEDAVVAENQDNFRLIRQHTTTPLA
                     VGEIFSSLWDCKELIQNQWIDYIRATIVHAGGITHLRRIAAFADVFGVRTGCHGATDL
                     SPVTMAAALHVGRSIPNFGVQEYMRHTDA"
     gene            1547..>2366
                     /locus_tag="VF402_05595"
     CDS             1547..>2366
                     /locus_tag="VF402_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7799803.1"
                     /translation="MKTINGRRRALRTALLGATILAAAGLSVTQAADAQDAAPTAAPA
                     PASGDSTVVVVTGYRASLENSTLAKKRAVGFQDSIYAEDIGKFPDTNIAESFNRIPGV
                     IISRDINGDGVNVSIRGLGTSFTRVLLNGAPVAVASTGSTDSQNTNREVDLDLFPTEL
                     FTSLTVDKSPQASEVEGGAAGTVNMRQARPFDHKGPQLVWSVGGTKSENADGWGNKGS
                     LVASNTFGNWGVLAGISFTNQKVANKGFETIGWTNANLSAAQDTLANRNSTGGGN"
BASE COUNT          450 a          798 c          732 g          386 t
ORIGIN      
        1 cgcgtcggtg tggcgcatat attcctgaac gccgaagttc gggatcgagc gcccgacgtg
       61 cagcgccgcc gccatcgtga ccggcgacag gtcggtggcg ccgtggcagc cggtgcgcac
      121 cccgaacacg tccgcgaagg cggcgatgcg gcgcaggtgc gtaatgccgc ccgcatgcac
      181 gatcgtggcg cggatatagt cgatccactg gttctggatc agctccttgc agtcccacag
      241 ggacgagaag atctcgccca cggccagcgg cgtggtggtg tgctggcgga tcaggcggaa
      301 attgtcctga ttttccgcca cgaccgcgtc ctccagccag aacgggcggt acggctccag
      361 atcgcgcccc agccgcccgg cctcgatcgg cgtcaggcgg tgatgcacgt cgtgcagcag
      421 gtgcgtctcg tcgcccagcg cgtcgcgcgc ggcggcgaat agtttgggca ccacgcgcat
      481 atagctttcg gtgtcccaga cattttccgc cggcaggtcg gaatcggcgg gttcgtagaa
      541 cgccctgtcc ttggacacgc cataggtgct gctgaggccc ggcacgccgc attgcaggcg
      601 gatcgccttg taaccctgcg ccttatagct gtgcgcgacc tccaccgtct cctcgatgga
      661 gacgccgttg gcgtggccgt aaaccatcat gccgtcgcgg caggcgccgc ccagaagctg
      721 atacaggggc aggcccgcga tcttggcctt gatgtcccac agcgccatgt cgaccgccgc
      781 gatggccgtc atggtcaccg gcccgcgccg ccagtaggcg ccgcgataga gatattgcca
      841 gacatcctcg attccatgcg cgtcgcgccc gatcaggcag ggaatcacat gcgcctcaag
      901 ataggccgcg acggcctttt cgcgcccgtt caacgtcgcg tcgccgacgc cgtacacgcc
      961 ctgatcggtc tcgatcttca gcgtgacgaa gttgcggccc ggacaggtga cgatgacccg
     1021 cgccgaaacg atgcgcgcgc gcgcaggcgc ggagggcgga gagggagcag gcgcaagctt
     1081 gggcaacatg tcggaaaagt cctgaggcgc gcacgggaga aaaatgcgcc gctaattggt
     1141 aagaccatta gacccaaaca tatcttgtcc gcaagcctgc accggaggaa atttatcccc
     1201 gcgccttccg acgccgcacg cgcatgctct cgtcgccctg aattttacgg tgattattca
     1261 tgcctttatg cgcatattcc catatatgcg cccttacgat cgccgccggg tcatgtctga
     1321 cgccgcccgc ccgccgctgc aatgcgcgtg ataaaaatgt cgaaattcct atcaagatgg
     1381 ttatttaagg gtaagaccaa attgacaaca ttcatctgcg cggatacagg ttcgtcatgg
     1441 tgtgagcggt aacaccgccc gccggccgta aaacaataag ccacagcgtc ccctgaccaa
     1501 agggacaatc agggttcagg ccgcaaggcc cgcaaaggag aacgtcatga agaccatcaa
     1561 cgggcgccgc cgcgcgctca ggaccgccct gctgggcgcc accatactgg ccgccgcggg
     1621 tcttagcgtc acgcaagccg ccgacgcgca ggacgcggcg cccaccgccg cccctgcccc
     1681 cgcttcgggc gactccaccg tcgtcgtggt caccggctac cgcgcctcgc tggaaaactc
     1741 gaccctggcc aagaagcgcg ccgtcggctt tcaggattcg atctacgccg aggacatcgg
     1801 caagttcccc gacaccaaca tcgccgaatc gttcaaccgt attccgggcg tgatcatctc
     1861 gcgcgacatc aacggcgacg gcgtcaacgt ctcgatccgg ggcctgggca ccagcttcac
     1921 gcgcgtgctg ctgaacggcg cgccggtggc ggtggcctcg accggctcga ccgattcgca
     1981 gaacaccaac cgcgaagtcg acctcgacct gttcccgacc gagctgttca cctcgctgac
     2041 cgtcgacaaa agcccgcagg ccagcgaagt cgaaggtggc gccgccggca cggtcaatat
     2101 gcgtcaggcc cgtccgttcg accacaaggg gccgcaactg gtctggtcgg tcggcgggac
     2161 caagagcgag aacgccgacg gctggggcaa caaggggtcg ctggtcgcct ccaacacctt
     2221 cggcaactgg ggcgtgctgg ccgggatctc gttcaccaac cagaaggtcg ccaacaaggg
     2281 ctttgagacc atcggctgga ccaacgccaa tctgagcgcg gcgcaggaca ccctggccaa
     2341 ccgcaactcg accggcggcg gcaact
//
LOCUS       DASVLL010000336         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_290830,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000336 DASVLL010000000
VERSION     DASVLL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290830"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VF402_05600"
     CDS             <1..1111
                     /locus_tag="VF402_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7799804.1"
                     /translation="PDRLAVRAALETAQREGRAEIGFTPHAALDHYVALSLRKGADGR
                     LYGVLRDASLQHAHEAALEAARGEAEAMNAGKTRFVASMSHELRTPLNAVIGFSDIMR
                     QKLFGDLPPKYAEYAQLIWESGQHVLDLINDILDMSKIEAQKYELTLESFDAREPASQ
                     SVRLMRGQAHDKGVEIVVLPPPGALNVRSDRRAIKQICLNLLSNAIKFTPRGGQVTLE
                     MKAAQDSFDIVIRDTGIGIGPEDLSRIGQPYEQGGSSEQRAQGTGLGLSIVKAMAHML
                     GGTTRIDSTPGEGTCVTVTLPLGGGEADAEQPSLPLSGVAPSSAPASASAGMEALEAF
                     ARAALHPAYNPLEDIHHTPRNVDGFGEFVIRPPRS"
     gene            complement(1112..>1996)
                     /locus_tag="VF402_05605"
     CDS             complement(1112..>1996)
                     /locus_tag="VF402_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799805.1"
                     /translation="SDAVTAVSVSRYFLALADDRCHALQPDAAAALKAGYVQARNAAL
                     RAGHSMDDLGPWLQRARAAAAAVPCGDAKLTKDFADAGAAYRRFIVQTRLDLPGVRAG
                     WTADRAYGDDARWRLVQYQNLPDADVALGLYGPLDAPRFTVMADFHDGEKPYAARLLT
                     RDPDRLGSGLIDAGAFALTPAAPAGFGSDSLSFMARDSAEVRVALRPEIKTNLAGFTL
                     TGQHVAETAGDAVRFDFPSRAWPAIARLDPREDMVVEFDFDDGPRYARFEVGDFVTGL
                     TYVTLPQPYTHNRRDTAN"
BASE COUNT          312 a          637 c          778 g          269 t
ORIGIN      
        1 ccccgaccgg ctggccgtgc gcgcggcgct ggagacggcg cagcgcgagg gccgggccga
       61 gatcggcttc acgccgcacg ccgcgctgga ccactatgtc gcgctcagcc tgcgcaaggg
      121 cgcggacggg cggctgtacg gcgtgctgcg cgacgcctcg ctgcaacacg cgcacgaggc
      181 ggcgctggag gcggcgcgcg gcgaggccga ggcgatgaac gcgggcaaga cgcgcttcgt
      241 ggccagcatg agccacgaac tgcgcacgcc gctcaacgcg gtgatcggct tttccgacat
      301 catgcggcaa aagctgttcg gcgacctgcc gccgaaatac gccgaatatg cgcaactgat
      361 ctgggaatcg ggccagcacg tgctggacct gatcaacgac attctcgaca tgtccaagat
      421 cgaggcgcag aagtacgagc tgacgctgga aagcttcgac gcgcgcgaac cggcgtcaca
      481 gagcgtgcgg ctgatgcgcg ggcaggcgca cgacaagggg gtggagattg tcgtcctgcc
      541 gccgccgggg gcgctcaacg tgcgctccga ccggcgcgcg atcaagcaga tctgcctgaa
      601 cctgttgtcc aacgcgatca agttcacgcc gcgcggcggg caggtgacgc tggagatgaa
      661 ggccgcgcag gacagtttcg acatcgtgat ccgcgacacc ggcatcggca tcgggccgga
      721 ggatctgagc cgcatcggcc agccctacga gcagggcggc tcgtccgagc agcgcgcgca
      781 gggcacgggg ctgggcctgt ccatcgtcaa ggcgatggcg cacatgctgg gcgggacgac
      841 gcggatcgac tcgacgccgg gcgaggggac gtgcgtgacg gtgaccctgc cgctgggggg
      901 cggggaggcg gacgccgagc agccgtcgct gccgctgagc ggggtggcgc cgtcgtctgc
      961 gcctgcgtcg gcatctgccg gaatggaggc gctggaggcg ttcgcgcgcg cggcgctgca
     1021 tccggcgtac aatccgctgg aggacatcca tcacacgccg cgcaatgtcg acggcttcgg
     1081 cgagttcgtg atccggccgc ccagaagctg atcagttcgc cgtgtcgcgc cggttgtgcg
     1141 tatagggctg cggcagggtg acgtaggtca ggccggtgac gaagtcgccg acctcgaagc
     1201 gcgcatagcg cgggccgtcg tcgaaatcga attcgaccac catgtcctcg cgcgggtcga
     1261 ggcgcgcgat ggcgggccag gcgcggctgg ggaagtcgaa gcgcacggcg tccccggcgg
     1321 tttcggcgac gtgctggccg gtcagggtga aaccggcgag gttggtcttg atttcggggc
     1381 gcagcgcgac gcggacctcg gcggaatcgc gcgccatgaa gctgagcgaa tccgatccga
     1441 aaccggcggg cgccgccggg gtcagggcga aggcgcccgc gtcgatcagg ccggagccga
     1501 gccggtcggg atcgcgcgtc aacaggcgcg cggcgtaggg cttttcgccg tcgtggaaat
     1561 cggccatcac ggtgaagcgc ggcgcgtcga gcgggccgta gaggccgagc gcgacatcgg
     1621 cgtcgggcag attctgatac tggaccagcc gccagcgggc gtcgtcgcca taggcgcggt
     1681 cggcggtcca gcccgcgcgg acgccgggca ggtcgaggcg ggtctggacg atgaagcggc
     1741 gataggcggc gcccgcgtcg gcgaagtcct tagtcagttt cgcatcgccg cacgggacgg
     1801 cggcggcggc ggcgcgcgcg cgttgcagcc acgggccgag gtcgtccatg ctgtgaccgg
     1861 cgcgcagggc ggcgttgcgc gcctgcacat agcccgcctt cagcgcggcg gcggcgtccg
     1921 gttgcagcgc gtggcagcgg tcgtcggcca gggccaggaa atagcggctc acgcttacgg
     1981 cggtgaccgc gtcgct
//
LOCUS       DASVLL010000337         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_452355,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000337 DASVLL010000000
VERSION     DASVLL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452355"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VF402_05610"
     CDS             <1..772
                     /locus_tag="VF402_05610"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7799806.1"
                     /translation="ELADKISETLSFMSAIGISSESQQEVRQVDFFTSHEALLLNYEQ
                     AMTRVDSTSGDWYDTSAHMLWIGERTRQLDGAHVNYMKGIKNPIGVKCGPTMEPDDLK
                     RLIDALNPEAVPGRLTLIGRFGSDKVAERLPRLMAATKAHGVPVVWSIDPMHGNVVKA
                     NNGYKTRPFDRILSEVRSFVDVAAAEGVHPGGMHLEMTGQNVTECTGGAFALSEDELS
                     MRYHTHCDPRLNADQALELSFLVAERLHNLKLKARAAA"
     gene            complement(871..2109)
                     /locus_tag="VF402_05615"
     CDS             complement(871..2109)
                     /locus_tag="VF402_05615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX7799807.1"
                     /translation="MLKLFHDDALACLDRLTRHRWRRVVFSLFFLFPIVGNLIARMNK
                     HGWWLNDFDALICGADHIRRGLSPYDLHPVCAATRPAVYVYAPQVAQVFAPMSAWGLE
                     TARLVWWIPSLIAMGYLLWYAVLTPVKDAPWRLRLMTLAAIVGSVYACGNIGLILHAL
                     VTAAAVLWLADKARPKRWPFVLMVILSALVKPVMLTYLVVLLFERRPLPHRLGGAAVA
                     SLAGLAAIALEMLSAGTFSAEWRRTLNVIVMTEQPGISYFGWLNWLGLPPGDHLALAV
                     AALLMLTTLLAGWTLAESREDGMSRLAIGLGMAQLLNPRLMDYDMMALAPLMAIVIVA
                     SRPLGKRFFWAVSWAFTAILTVDVLTNIFEIKSFARSPVTLFIYWVMLLAVAARLLVT
                     QKRPEGLPGVFRSLWRGASA"
     gene            complement(2113..3276)
                     /locus_tag="VF402_05620"
     CDS             complement(2113..3276)
                     /locus_tag="VF402_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799808.1"
                     /translation="MFKRAAPFAALLSRHAVWLFALPPAFGVAAKLSRSKLWFTDYEA
                     VACAGQKVLAGHPIYDLNLACPGMHAASFVYLPGVAQSAAALQSVFGPSGFFLLYLGC
                     YLAAVAALTLLPLRAAPLADGRAQIPFLSFLSGSAFMGGNIAVLLHGGVLLAALAWLE
                     SAPWLVVAVIVVAAWVKPVFLAYLALILLADLSWRRRAGMMACGVAAGLAPSLIFALS
                     GGDLAHQWMSLLAHFVYDETPGRAWFGWLQMAGVEPDSLSAKAGYFAYAAALTGAVLA
                     LSRMLDLNRRERLWLGFSLAVLLIPRLMSEDVFLLGPGLILVAGRATAAGLTPRGDAV
                     VAALCCLCLGGGLTEMADVTTPLALLGFALYLMWAAFGLGLRSLRPMNSTVNG"
     gene            3473..>4587
                     /locus_tag="VF402_05625"
     CDS             3473..>4587
                     /locus_tag="VF402_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7799809.1"
                     /translation="MADTETHTGADAPDLSLIICTLNEGVAIRGVIEEISDALGGIRH
                     EIIVVDDNSADNTAAEVMAAAAERPNVRLHVRVGERGLSSAAIKGWDIAHGRYLGVMD
                     GDGQHDPHAIRALAEMIMKGDKDLVCVSRYLGATDTGLSGIRDIGSKAATFATGLVLK
                     VPLSDPMSGCFIMTRDYYQSARPKLTGIGFKILLDIAASAPKKPRFGEVKAALRQRQG
                     GQSKLDLRVVADLGALLIEKATKGVLPARFVLFAGVGVTGVFVYATVLIAFHYLLKSS
                     HGAPIYRYQYRLDDIISYSMAIWLSMTWNFFLNNVLTFRDKRLKGWRVLQALLGFYVA
                     CSLGALLSLALSIFMKDHLHIHWLVAGVSAALISSIW"
BASE COUNT          868 a         1528 c         1522 g          669 t
ORIGIN      
        1 tgaactggcc gacaagattt ccgagacgct gagcttcatg tcggccatcg gcatctcgtc
       61 ggaaagccag caggaggtgc gccaggtcga tttcttcacc tcgcacgaag ccctgctgct
      121 caattacgag caggcgatga cgcgcgtcga ttcgacgtcg ggcgactggt acgacacctc
      181 ggcgcacatg ctgtggatcg gcgagcgcac gcgccagctt gacggcgcgc acgtcaacta
      241 catgaagggc atcaagaacc cgatcggcgt caagtgcggc ccgaccatgg agccggacga
      301 cctgaaacgc ctgatcgacg cgctgaaccc cgaggcggtt ccgggccgtc tgaccctgat
      361 cgggcgcttc ggctcggaca aggtggccga gcgtctgccg cgcctgatgg cggcgaccaa
      421 ggcgcacggc gtgccggtgg tctggtccat cgacccgatg cacggcaatg tcgtcaaggc
      481 caataacggc tacaagacgc gtccgttcga ccgcatcctc agcgaggtgc gcagtttcgt
      541 cgacgtcgcg gcggcggagg gcgtgcatcc gggcggcatg catcttgaga tgaccggcca
      601 gaacgtcacc gaatgcacgg gcggcgcctt tgcgctgagc gaggacgagc tgagcatgcg
      661 ttaccacacg cattgcgatc cgcgcctcaa cgccgatcag gcgctggagc tgagcttcct
      721 ggtcgccgag cggctgcata acctgaaact gaaggcgcgc gcggcggctt agagcgcttt
      781 ccgaaaagtg tgacgcactt ttcggattaa aaagcgcgta aaagcataaa gttagagcgc
      841 cgatctgatg caatcagatc ggaaagcgct ctaagcggac gcgccgcgcc acagcgacct
      901 gaaaacgccc ggaagcccct ccgggcgttt ttgcgtgacc agcaggcgcg ccgcgaccgc
      961 cagcagcatg acccagtaga tgaacagggt gaccggcgag cgcgcgaacg acttgatctc
     1021 gaagatgttg gtcagcacgt ccacggtcaa aatggcggtg aaggcccagc tcaccgccca
     1081 gaagaagcgc ttacccagcg gccgcgacgc cacgatcacg atggccatca gcggcgccag
     1141 cgccatcatg tcgtagtcca tcaggcgcgg attgagcagt tgcgccatgc ccagcccgat
     1201 ggcgaggcgg ctcatgccgt cctcgcggct ttcggccagc gtccagcccg ccagcagcgt
     1261 cgtcagcatc agcagcgcgg ccacggccag cgccagatga tcgcccggcg gcaggcccag
     1321 ccagttcagc cagccgaaat agctgatgcc cggctgctcg gtcatgacga tgacgttcag
     1381 cgtcctgcgc cactccgcgc tgaaggttcc ggcgctcagc atttcgagcg cgatggcggc
     1441 cagacccgcc aggctggcga ccgccgcgcc gccaaggcga tgcggcagcg ggcggcgctc
     1501 gaacagaagc acgaccagat aggtcagcat gaccggcttg accagcgcgg acaggatcac
     1561 catcagcacg aacggccagc gcttcgggcg cgccttgtcg gccagccaca gcaccgccgc
     1621 cgccgtcacc agcgcgtgca ggatcaggcc gatattgccg caggcataga cgctgccgac
     1681 gatggcggcc agcgtcatca gccgcagccg ccacggcgcg tccttcaccg gcgtcagcac
     1741 cgcgtaccac agcaggtagc ccatcgcgat caggctgggg atccaccaga ccagccgggc
     1801 cgtctccagc ccccacgccg acatcggcgc gaagacctgc gccacctgcg gcgcatagac
     1861 atagacggcg ggccgcgtgg ccgcgcacac cggatgcagg tcgtagggcg acaggccgcg
     1921 ccggatatgg tcggcgccgc agatcagcgc gtcgaaatcg ttgagccacc agccatgctt
     1981 gttcatgcgg gcgatcaggt tgcccacgat cgggaacagg aagaacaggc tgaagaccac
     2041 gcgccgccac cgatgccggg tgaggcggtc gagacaggcg agggcgtcgt cgtgaaagag
     2101 cttcaacatg gcttatccat tcaccgtgct gttcatcggg cgcagagagc gcaggccgag
     2161 gccgaacgcc gcccacatca ggtagagcgc gaaaccgagc agggccagcg gcgtggtcac
     2221 gtccgccatt tcggtcaggc cgccgcccag gcacaggcag cacagggccg cgaccaccgc
     2281 gtcgccgcgc ggcgtcaggc ccgccgccgt ggcgcgtccg gcgacgagaa tcaggcccgg
     2341 ccccagcagg aacacgtcct cgctcatcag gcgcgggatc agcagcacgg cgaggctgaa
     2401 gcccagccac aggcgctcgc gccggttcag gtccagcatc cggctcagcg ccagcaccgc
     2461 gccggtcagg gcggcggcgt aggcgaagta accggccttg gcgctgagcg aatccggctc
     2521 gacgcccgcc atctgcaacc agccgaacca cgcccggccg ggcgtttcgt catagacgaa
     2581 atgcgccagc agcgacatcc actgatgcgc cagatcgccg ccgctcagcg cgaagatcag
     2641 gctgggcgca aggcccgcgg ccacgccgca ggccatcatg cccgcccgcc tgcgccacga
     2701 caggtcggcc agcaggatca gcgccagata ggccagaaag accggcttca cccaggccgc
     2761 gaccacgatc acggcgacga ccagccacgg cgcgctctcc agccaggcca gagccgccag
     2821 cagaacgccg ccgtgcagaa gcaccgcgat attgccgccc atgaaggcgc tgccgctcag
     2881 aaagctcagg aacggaatct gcgcgcgccc gtcggccagc ggcgcggcgc gcagcggcag
     2941 cagggtcagc gccgcgacgg cggccagata gcatcccaga tacaaaagga agaagccgga
     3001 cggaccgaac accgattgca gggcggcggc ggactgcgcc acgccgggca gatagacgaa
     3061 gctggccgcg tgcatgccgg ggcaggcgag gttgagatcg tagatcgggt ggcccgccag
     3121 aaccttctgc ccggcgcagg ccacggcctc gtaatcggtg aaccacagct tcgagcggct
     3181 cagcttggcc gcgacgccga acgcgggcgg cagggcgaac agccagacgg catggcggga
     3241 cagaagggcg gcgaaaggcg cagcgcgttt aaacattgtc ttccggcggt gaaagcgata
     3301 aggagcgggt tttgctgttc gccgcttagc gggcgcatag gacggtaatt tgaccggcat
     3361 gtgtcaggtc tgatgccgag ttcggcggat cgttcataga ttcgccaccg ttcgaccttt
     3421 taagcggcgt gacggaaccc gtgggccggg ccgatcgccg gggagttctt ttttggccga
     3481 taccgaaacg catacgggcg cggacgcgcc cgacctcagc ctgatcatct gcacgctgaa
     3541 cgaaggcgtc gccattcgcg gcgtgatcga ggagatttcc gacgcgcttg gcggcatccg
     3601 ccacgagatc atcgtcgtcg acgacaattc cgccgacaac acggcggccg aggtcatggc
     3661 ggccgcggct gagcgtccga acgtgcgcct gcatgtgcgc gtcggcgaac gcggcctgtc
     3721 ctcggcggcg atcaagggct gggacatcgc gcacggccgc tatctcggcg tcatggacgg
     3781 cgacggccag cacgatccgc acgcgatccg cgccctggcc gagatgatca tgaagggcga
     3841 taaggatctg gtctgcgtgt cgcgctatct cggcgcgacg gacaccgggc tgtccggcat
     3901 ccgcgacatc ggctccaagg cggcgacctt cgccacgggg ctggtgctga aggtgcctct
     3961 gtccgatccg atgagcggct gcttcattat gacgcgcgac tattatcagt cggcgcggcc
     4021 caagctgacc gggatcggct tcaagatttt gctcgacatc gccgcgtccg cgccgaaaaa
     4081 gccgcgcttc ggcgaggtca aggcggcgct gcgccagcgt cagggcggcc agtccaagct
     4141 ggacctgcgc gtggtcgccg acctcggcgc gctgctgatc gagaaagcca ccaagggcgt
     4201 gctgcccgcg cgcttcgtgc tgttcgcggg cgtcggcgtg accggcgtgt tcgtctatgc
     4261 gacggtgctg atcgcgttcc actacctgct caagagcagc cacggcgcgc cgatctatcg
     4321 ttaccagtac cgtctcgacg acatcatcag ctacagcatg gcgatctggc tgtcgatgac
     4381 gtggaacttc ttcctcaaca acgtcctgac cttccgcgac aagcggctca agggctggcg
     4441 ggtcttgcag gcgctgctcg gcttctacgt cgcctgttcg ctgggcgcgc tgttaagtct
     4501 ggcgctgtcg atcttcatga aggaccatct gcacattcac tggctggtcg ccggggtcag
     4561 cgcggcgctg atcagttcga tctggaa
//
LOCUS       DASVLL010000338         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_75324, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000338 DASVLL010000000
VERSION     DASVLL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75324"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF402_05630"
     CDS             complement(<1..308)
                     /locus_tag="VF402_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799810.1"
                     /translation="MMCLLPAHAAAACHVRLPRHSESVLSADKRHIAFIRQVPSVNVP
                     DSQAGELWLGDCASGETRRLLPARFSGVEATSQWTSLGSPVFSSDSRHVFVNAAYGGD
                     "
     gene            complement(335..1531)
                     /locus_tag="VF402_05635"
     CDS             complement(335..1531)
                     /locus_tag="VF402_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX7799811.1"
                     /translation="MKRLFALFALLSLMLNPGAPARAADLIGFWDAPRHGADSFNEAP
                     PDRAYFQALRATGATWVRLTFSKWQGAGRDFLIGDADHYTGIPASDLSVLRRVLRDAD
                     AAGLKVVIAPLSLPGDRWSQQNGNKDDTRLWNDRAWWDQSAAFWRDLAAALKDEPMIA
                     GYNLINEPTPEKGMGLDEQAAPDARKAWYARYKGTTHDLPAFYETLIRAIRAVDPDTP
                     VMVDGGWYANAWSFSYWPSKLSDDKALYSFHMYEPYEATSGPNIKRKPQLRYPGVESW
                     FGGQKVRWNKAVMTRYLSGPFDWAAAHGVPGNRMVAGEFGCVRAWVDCGAYLSDVLDV
                     LKSHHAHWAFYSWREDGWDAMDYELAPSVTAGQFYYLHEQGKADQLKRAPHPLLDIIE
                     AHMGDD"
BASE COUNT          261 a          560 c          540 g          288 t
ORIGIN      
        1 tcatcgccgc cataggcggc gttgacaaag acatgtcggc tgtcggacga gaaaacgggc
       61 gaccccagcg aagtccattg cgaggtcgct tcaacgccgc tgaaacgcgc aggcagcagg
      121 cggcgcgtct cgccgctggc gcaatcgcca agccagagtt cgcctgcctg gctgtcggga
      181 acgttcacgc tgggaacctg ccggatgaag gcgatatggc gcttatctgc tgacaggacg
      241 gattcgctgt ggcggggaag ccggacatgg catgctgcgg ccgcgtgcgc aggcaggagg
      301 cacatcatgg cgaaccagac cctgaggccg cgcatcaatc gtcgcccata tgcgcttcga
      361 tgatgtcgag cagcgggtgc ggcgcgcgtt tcaattgatc cgccttgccc tgttcgtgga
      421 ggtaatagaa ctgtcccgcc gtcaccgagg gcgccagttc gtagtccatc gcgtcccagc
      481 cgtcttcgcg ccaactgtag aacgcccagt gcgcgtgatg gcttttcagc acgtcgagca
      541 cgtcgctcag gtacgcgccg caatcgaccc atgcgcgcac gcagccgaat tcgcccgcca
      601 ccatgcggtt gccgggcacg ccatgcgcgg cggcccagtc gaacgggccg ctcaagtacc
      661 gcgtcatcac cgccttgttc cagcggacct tctggccgcc gaaccagctc tccacgcccg
      721 gatagcgcag ttgcggcttg cgtttgatat tggggccgct ggtggcctcg tagggttcgt
      781 acatgtggaa gctgtacagc gccttgtcgt cgctgagctt cgacggccaa tagctgaaac
      841 tccatgcgtt cgcgtaccag ccgccgtcca ccatcaccgg cgtgtcggga tcgacggcgc
      901 ggatcgcccg gatcagcgtc tcgtaaaagg cgggcaggtc gtgcgtcgtg cccttatacc
      961 tggcgtacca cgccttgcgc gcgtcgggcg cggcctgttc gtcgaggccc atgccctttt
     1021 ccggcgtcgg ctcgttgatc aggttgtagc ccgcgatcat cggctcgtcc ttcagcgcgg
     1081 cggccagatc gcgccagaag gccgccgact ggtcccacca ggccctgtcg ttccacagcc
     1141 gcgtgtcgtc cttgttgccg ttctgctggc tccagcggtc gccgggcagg gacagcggcg
     1201 cgatcaccac cttcagcccc gccgcgtccg cgtcgcgcag cacgcggcgc aggacggaga
     1261 gatcggacgc cggaatgccg gtgtaatggt ccgcgtcgcc gatcaggaag tcgcggcctg
     1321 cgccctgcca tttcgagaag gtcagccgca cccaggtcgc gcccgtcgcg cgcagggcct
     1381 gaaaataggc ccggtcgggc ggcgcttcgt tgaagctgtc ggcgccgtgg cgcggcgcgt
     1441 cccagaagcc gatcaggtcg gcggcgcgcg cgggcgcgcc cggattcagc atcaggctta
     1501 gcagggcgaa cagggcgaac aggcgcttca tcttgtctcc cccggtccgg agcgtcaccg
     1561 ctccgtcggc aatccggcca tcttccacgc atacagcccg ccggccagat gatgcttcac
     1621 gtcgaggccc gtggcttgca gatactccg
//
LOCUS       DASVLL010000339         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_235883,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000339 DASVLL010000000
VERSION     DASVLL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235883"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..150
                     /locus_tag="VF402_05640"
     CDS             13..150
                     /locus_tag="VF402_05640"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799812.1"
                     /translation="MLDAGVDGAADALMDKILSVASGAPTRNEINDEREIAIWKNGVT
                     L"
     gene            180..1592
                     /locus_tag="VF402_05645"
     CDS             180..1592
                     /locus_tag="VF402_05645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX7799813.1"
                     /translation="MSPAVTGPVYDPSSVGVGIVHFGPGAFFRAHQAAYVDALLASDA
                     RWGICGVSLHSAGVRDALVPQDGLYTLAVLDEQVSYRVIGALREVLVAPEDPEAVFAR
                     LTRPEVRVVTSTVTEKGYCLGAGFALDFDHPDIAHDLAHPRTPRSFIGYVVEGLRRRR
                     ESGQAPFVLIPCDNLPDNGRRLKAALAALAAKSDAGLAAWIGEKLTCPCTMVDSITPA
                     TDDALRARVTEATGLEDNWPIQREAFTQWVIEAHGNLDGPDWAGVGVTLTDNVPAFER
                     AKLRLLNGPHSTLAYVGSLKGYETVYEAMQDAELAGFVRALMLEDIAPLLEAPSGMDL
                     AGYCEAILRRFRNPAIRHLLAQIAWDGSQKLPIRILSSLGEALDRGRDIRRFCAPLAA
                     WMRFVVGKARRGEAIVDPLADRLTAIGAACDDSPADVDRFLSLTEVFPQALAGNAVFR
                     EAVRGAYADCAKLNRIAAPA"
BASE COUNT          221 a          464 c          649 g          281 t
ORIGIN      
        1 gacgccgggg tcgtgctgga cgcgggcgtg gacggcgcgg cggacgcgct gatggacaag
       61 attctgtcgg tggcctcagg cgcgccgacg cgcaacgaaa tcaacgatga gcgcgaaatc
      121 gccatctgga agaacggagt caccctgtga cgcagcttaa cctccccgcc ctctccgccc
      181 tgtcgcctgc tgtaacgggt ccggtttacg atccttcatc ggtcggggtg gggatcgtgc
      241 atttcgggcc tggggcgttt tttcgggcgc atcaggcggc ctatgtggac gctttgctgg
      301 cgtcggacgc gcgctggggg atctgcgggg tgtcgctgca ttcggcgggg gtgcgcgacg
      361 cgcttgtccc gcaggacggg ctttacacgc tggcggtgct ggacgagcag gtcagctacc
      421 gggtgatcgg ggcgttgcgc gaggttctgg tcgcgccgga agaccctgag gcggtgttcg
      481 cgcggctgac gcggccggag gtgcgggtgg tgacctcgac ggtgacggag aagggctatt
      541 gcctgggggc gggcttcgcg ctggacttcg atcatcccga catcgcgcac gatctggcgc
      601 atccgcggac gccgcggagc tttatcggct atgtggtgga gggcctgcgc cgccgccgcg
      661 agagcggtca ggcgccgttc gtgctgatcc cgtgcgacaa cctgccggac aatggcaggc
      721 ggctgaaggc ggcgctggcg gcgctggcgg cgaagtcgga cgcggggctg gcggcgtgga
      781 tcggcgagaa gctgacctgc ccgtgcacca tggtcgattc gatcaccccg gcgacggacg
      841 acgccttgcg ggcgcgggtg acggaggcga ccggcctgga ggacaactgg ccgatccagc
      901 gcgaggcgtt cacgcagtgg gtgatcgagg cgcacggtaa tctggacggg ccggactggg
      961 ccggcgtcgg ggtgacgctg accgacaatg ttccggcgtt cgagcgggcg aagctgcggc
     1021 tgctgaacgg gccgcattcg accctggcct atgtggggtc tttgaaggga tatgagacgg
     1081 tctatgaggc gatgcaggac gctgagcttg ccgggttcgt gcgcgctctg atgctggagg
     1141 acatcgcgcc cttgctggaa gcgccttcgg ggatggacct ggcgggctat tgcgaggcga
     1201 tcctgaggcg gttccgcaac ccggcgatcc ggcacctttt ggcgcagatc gcgtgggacg
     1261 gttcgcagaa gctgccgatc cggatactgt cgtcgctggg cgaggcgctg gatcggggga
     1321 gggacatccg ccgtttctgc gcgcctctgg cggcgtggat gcggttcgtg gtcggcaagg
     1381 cgcgtcgggg cgaggcgatc gtcgacccgc tggcggatcg gctcaccgcg atcggcgcgg
     1441 cgtgcgacga cagtccggcg gatgtggacc ggttcctgag cctgacggag gtgtttcccc
     1501 aggcgctcgc cgggaatgcg gtgttcaggg aggcggtgcg gggggcgtat gccgattgcg
     1561 cgaagctgaa ccggatagcg gcgccggcat aggtcggcgc gctttcggcg gcgcg
//
LOCUS       DASVLL010000340         4597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_130032,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000340 DASVLL010000000
VERSION     DASVLL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130032"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..576)
                     /locus_tag="VF402_05650"
     CDS             complement(148..576)
                     /locus_tag="VF402_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7799814.1"
                     /translation="MSRLIVHATCLSLYTGGAWRGALILGPSGAGKSDLALRAMQAGC
                     ALISDDYTRLWSSHGHLYAGAPETIAGRMEIRGLGVVGAAARPFTRVHLAALAQDETP
                     ERLPAAEVTALLGHAVPTLRVTPREASSLAKLLTALRGRV"
     gene            complement(579..2177)
                     /locus_tag="VF402_05655"
     CDS             complement(579..2177)
                     /locus_tag="VF402_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7799815.1"
                     /translation="MARTDAADPRPSPRASSIHLTFGRSRLGRLIFGLNLIGLLIIAV
                     GALVLSDLRRGLILSQMDSLTSQAQVVGEVIAEVATEGEPEPTLDEDNALAALARFIP
                     PGERARLFDAHGKLISDSYVVSDSVDVSPLPPARKRDDPAPADKSALKAARRRTAEAQ
                     LHRDVAVALTGRVWASVRLDERGKKVVSVSIPLRRVKEVLGVLTLERGDVDKIVWAQR
                     WAMAPFILVAFAVAMLSSLLLFWLVSRPIDRLSAAADRVRMAQTRAISLPDLESRKDE
                     IGALARSLESMTETLWRRMEEIDRFAADVSHEIKNPLTSIRSALETLPMVKDEAARER
                     LMNVVAQDVRRLDRLITDISNASRLDAELSRDTPRKVDIGALLEDIVSLYQQSHLPGQ
                     AGVGLTRDLPAGVRVSGREGPLGQVFRNLIDNARSFSPEGGAEDSLVRVAVRPSGDPA
                     LPVAVVVEDDGPGIPEENLETVFQRFYTSRPKGARFGQNSGLGLSISRQIVEAHGGRI
                     WAENRKDGEGRVTGARFTLHLPQA"
     gene            complement(2204..2914)
                     /locus_tag="VF402_05660"
     CDS             complement(2204..2914)
                     /locus_tag="VF402_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7799816.1"
                     /translation="MARITLVDDDENIVTSVSLALESGGHTIQAYYDGAAGLAALEKD
                     PPDLAILDVKMPRMDGMEVLRRLRATSNLPVIILTSKDDEIDEVLGFNLGADDYVHKP
                     FSQRLLLERVKAVLRRAGIVEQGEAPDVTEALNAKSLKRGKLVMDAARHECTWDGRPV
                     RLTVTEFLLLHALAQRPGFVKSRDNLMDAAYDDQVYVDDRTIDSHIKRMRKKFRVVDP
                     EFDSIETLYGVGYRYREA"
     gene            3097..>4597
                     /locus_tag="VF402_05665"
     CDS             3097..>4597
                     /locus_tag="VF402_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799817.1"
                     /translation="MTDRPVHYEVFSRRNVKTSWGLEMATESRDQALAAAEDLLREGR
                     VAVRVSKETFDSETGEFRSHRLLEKGVAAPLKKPDRIRETDSVCAGPADLYTAPAREK
                     IARLLEDWLRRYDATPFELLHSPHLAEKLEASGNDLLHVAQKLAVPESQDTGVPLHEL
                     IRRWQGLFDRACARLIQDGRKGLFPDLTPDSFAAAVDRMRDQPERAYILGGGLARALQ
                     DAKGPAAKLRALLVFAERLSGAAGERNWAMQVIEMPVVEIFSGRGSLADLTGEELDLG
                     GSLALLTQLAAGREVALIARHDARVARLMPPPDEVLAGYNRLIQDGHLPHLAHVIARR
                     CVSELKGPRRLCPGDALAEIETLRALALCMTAAGKDESQREDIKDAFVERSKKLVTSD
                     FVTALMEDVDDGAEEVERLIWLCENMVGAANKRQAARWLLTFAGQQKFERALREGPEP
                     PAHRLQTLARMQGRVRAAQQVDKDGDDIGVRLGQLGSLIAQDAKLIAHVR"
BASE COUNT          725 a         1543 c         1631 g          698 t
ORIGIN      
        1 ccaatccgat cataaagccc tgttcctaag cccttacagc ttccgcgggg gcaggtcgcc
       61 ggtgcggaag tccgccatca taagcccgat ttcatcgcac tgcaaaacag aattcgcgtg
      121 aggcggattg tgatggaaaa cggaaggtca gacccgcccg cgcagcgcgg tgagcagctt
      181 ggccagactg gaggcctcgc gcggcgtcac gcggagggtc ggaacggcgt ggccgagcag
      241 ggcggtgacc tcggcggcgg gcagccgctc cggcgtctcg tcctgcgcca gcgcggccag
      301 atgcacgcgc gtgaacggac gcgccgccgc gccgacgacg cccagcccgc gaatctccat
      361 gcgcccggcg atcgtctccg gcgcgcccgc gtacaggtga ccgtgcgacg accacaggcg
      421 ggtgtagtcg tcgctgatca gcgcgcatcc ggcctgcatg gcgcgcaggg ccaggtcgct
      481 cttgcccgcg ccggacggcc ccaggatcag cgcgccgcgc catgcgccgc cggtatagag
      541 gctcaggcag gtggcgtgga cgatcagacg gctcatgctc acgcctgcgg caggtggagg
      601 gtgaagcgcg cgccggtgac gcggccctcg ccgtccttgc ggttctcggc ccagatgcgg
      661 ccgccgtgcg cctcgacgat ctggcgcgag atcgacaggc cgaggccgga attctgcccg
      721 aagcgcgcgc ccttgggccg cgaggtgtag aagcgctgaa agacggtttc gaggttttcc
      781 tccggtatgc ccggcccgtc gtcctcgaca acgaccgcca ccggcagggc cggatcgccc
      841 gacggcctga cggcgacgcg cacgaggctg tcctccgccc cgccctccgg cgagaacgag
      901 cgcgcattgt cgatcaggtt gcggaacacc tgccccagcg ggccttcgcg gcccgagacg
      961 cgcacgcccg cgggcaggtc gcgcgtcagg ccgaccccgg cctgtccggg cagatggctc
     1021 tgctggtaca gcgagacgat gtcctccagc agcgcgccga tatcgacctt gcgcggcgtg
     1081 tcgcgcgaca gttcggcgtc gaggcgcgag gcgttggaga tgtcggtgat cagccggtcg
     1141 aggcggcgca cgtcctgcgc caccacgttc atcagccgct cgcgcgccgc ctcgtccttg
     1201 accatgggca gcgtctccag cgccgagcgg atcgaggtca gcggattctt gatctcgtgg
     1261 ctgacgtcgg cggcgaaacg gtcgatctcc tccatgcgcc gccacagggt ctcggtcatg
     1321 ctctccagcg accgggccag cgcgccgatc tcgtccttgc ggctttccag atcgggcagg
     1381 gagatggcgc gggtctgcgc catgcgcacc cggtcggcgg cggcgctcag ccggtcgatc
     1441 ggacggctga ccagccagaa cagcagcagc gacgacagca tggccacggc gaaggcgacc
     1501 agaatgaacg gcgccatcgc ccagcgctgc gcccagacga tcttgtcgac gtcgccgcgc
     1561 tccagcgtca gcacgcccag cacctccttg acgcggcgca gcgggatcga caccgacacc
     1621 accttcttgc cgcgttcgtc cagccgcacc gacgcccaga cgcgcccggt cagggcgacg
     1681 gcgacgtcgc ggtgcaactg cgcctcggcg gtccggcgcc gcgcggcctt gagcgccgac
     1741 ttgtccgccg gggccggatc gtcgcgcttg cgcgcgggcg gcaacggcga cacgtcgacg
     1801 gagtcggaga cgacgtagga atccgagatc agcttgccgt gggcgtcgaa caggcgcgcg
     1861 cgctcgcccg gcgggatgaa gcgcgccagc gcggccagcg cgttgtcctc gtccagggtc
     1921 ggctccggct cgccctcggt ggcgacctcg gcgatcacct cgccgaccac ctgggcctgc
     1981 gaggtcagcg aatccatctg gctgaggatc aggccgcgcc gcaggtccga cagcaccagc
     2041 gcgccgacgg cgatgatcag cagcccgatc aggttgaggc cgaaaatcag ccgccccagc
     2101 cgcgagcgcc cgaaggtcag gtggatggag gacgcgcgcg gcgaagggcg cggatcggcc
     2161 gcgtcggttc tggccatatc aggtcgcgcc ccgcatgcgg cgttcaggct tcgcgataac
     2221 gatagccgac gccgtacagc gtttcgatcg aatcgaattc gggatcgacg acgcggaact
     2281 tcttgcgcat ccgcttgatg tggctgtcga tggtgcggtc gtcgacatag acctgatcgt
     2341 cataggcggc gtccatcagg ttgtcgcggc tcttgacgaa gccggggcgc tgcgccagcg
     2401 cgtgcagcag caggaattcg gtcacggtca gccgcaccgg cctgccgtcc caggtgcatt
     2461 cgtggcgcgc cgcgtccatg accagcttgc cgcgtttcag cgacttggcg ttcagcgcct
     2521 cggtcacgtc gggcgcctcg ccctgctcga cgatgcccgc gcggcgcagg accgccttga
     2581 cgcgctccag caggaggcgc tgcgagaacg gcttgtgcac atagtcgtcg gcgccgaggt
     2641 tgaagcccag cacctcgtcg atctcgtcgt ccttcgaggt caggatgatg accggcaggt
     2701 tcgaggtggc gcgcaggcgg cgcagcacct ccatgccgtc catgcgcggc atcttcacgt
     2761 cgagaatggc caggtccggc gggtctttct ccagcgcggc cagaccggcg gcgccgtcgt
     2821 aataggcctg tatggtatgg ccgccgcttt ccagcgccag cgagacgctg gttacgatgt
     2881 tttcgtcgtc gtcgaccagg gtgattcggg ccatgcgatc tcctcgggag gcggttcatg
     2941 ctgcggtccg aaaaccgcgc gcgttcggat gatatcccat tttaccctaa gctggttctt
     3001 ttcagggcgt caacgcggcg aatttcggac tttcaaggat aatttaacgg gatgtgtgta
     3061 ggttttgtcg tcaaatcaag gggcttacag gccaaaatga cagaccgtcc cgtccattac
     3121 gaggtgtttt cgcgccgcaa cgtcaagacg tcgtggggac tggagatggc gacggagtcg
     3181 cgcgatcagg ccctggccgc cgccgaggat ctgctgcgcg agggccgcgt cgcggtgcgc
     3241 gtgtcgaagg agaccttcga tagcgaaacc ggcgaattcc gctcgcaccg cctgctggaa
     3301 aaaggcgtgg cggcgccgct gaaaaaaccg gaccgcatcc gcgaaaccga ttccgtctgc
     3361 gccggtccgg ccgacctcta caccgccccg gcgcgggaaa agatcgcgcg gctgctggag
     3421 gactggctgc gccgttacga cgccacgccg ttcgaattgc tgcacagccc gcacctggcc
     3481 gaaaagctgg aggcgtcggg caacgacctg ctgcacgtcg cgcagaagct ggccgtgccg
     3541 gaaagtcagg acacgggcgt gccgctgcac gaactgatcc ggcgctggca gggcctgttc
     3601 gaccgcgcct gcgcgcggct gattcaagac ggacgcaagg gcctgtttcc cgacctgacg
     3661 ccggacagct tcgcggcggc ggtcgaccgg atgcgcgacc agccggagcg cgcctatatt
     3721 ctgggcggcg gactggcgcg cgcgttgcag gacgcgaagg gaccggcggc caagctgcgc
     3781 gccctgctgg tcttcgccga gcgcctgtcc ggcgcggcgg gcgagcgcaa ctgggcgatg
     3841 caggtgatcg agatgccggt ggtggagatc ttctccgggc gcggcagtct ggccgacctg
     3901 accggcgagg agctggatct gggcggctcg ctggccctgc tgacgcaact ggcggcgggg
     3961 cgcgaggtcg cgctgatcgc taggcacgac gcgcgcgtgg cgcggctgat gccgccgccg
     4021 gacgaggtcc tggccgggta taaccggctg attcaggatg gccatctgcc gcatctggcg
     4081 catgtgatcg cccggcgctg cgtgagcgag ctgaaggggc cgcgccgcct gtgccccggc
     4141 gacgccctgg ccgagatcga gaccttgcgc gccctggccc tgtgcatgac ggcggcgggc
     4201 aaggacgaga gccagcgcga ggacatcaag gacgccttcg tcgagcgctc gaagaagctg
     4261 gtgacctcgg atttcgtcac ggcgctgatg gaggatgtcg acgacggcgc cgaggaggtc
     4321 gagcggctga tctggctgtg cgagaacatg gtcggcgccg ccaacaagcg tcaggcggcg
     4381 cgctggctgc tgaccttcgc gggccagcag aagttcgagc gcgcgctgcg cgaagggcca
     4441 gagccgcccg cgcaccgcct gcaaaccctg gcccggatgc agggccgcgt gcgcgctgcg
     4501 caacaggtcg acaaggacgg cgacgatatc ggcgtgcggc taggccagct cggctcgctg
     4561 atcgcgcagg acgcgaaact gatcgcgcac gtgcggc
//
LOCUS       DASVLL010000341         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_506530,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000341 DASVLL010000000
VERSION     DASVLL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506530"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VF402_05670"
     CDS             complement(<1..736)
                     /locus_tag="VF402_05670"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7799818.1"
                     /translation="MPFSDDIHQEIESYLGFLIDHELDDAYETEPLNRTLSENKQRIQ
                     RAPAAAAQIHVTPGPAAAPANALRPQSLAHLDLGAAAHEAKQRAHAARDFDELYAELE
                     AFQHMPLRHEGAKSLVRFRGVPAPALMVIGEPPDADEDETGLAFAGKPGEMLDKALHA
                     AGVAAQSLLAPCVPWRPAGGRPLTPEDVALSAPFLHALIRLARPKALLLLGAPAVACA
                     LNLDQSLSKLRGRVVGYHENGLQIPVV"
     gene            836..>1612
                     /locus_tag="VF402_05675"
     CDS             836..>1612
                     /locus_tag="VF402_05675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7799819.1"
                     /translation="MADTQDAVARESMDYDVVIVGGGPAGLSAAIRLKQKAAEAGTEV
                     SVAVLEKGSEIGAHILSGAVIDPSGLAKLFPDWQARGAPLETPVTDDRFLFLGPQGVM
                     DISWLPKPGYMSNHGCYIASLGNLSRWLAEQAEGLGVEIYPGMACSEVLYDADGGVAG
                     VVAGVFGVARDGHHKPDYQPGLELRARYTFFAEGARGSLSQQVIRRFELDKDSDCQKY
                     GLGVKELWKVRPEQFRPGLVQHTLGWPLDDRTGGGSFLYHF"
BASE COUNT          250 a          523 c          578 g          261 t
ORIGIN      
        1 cgaccaccgg gatttgcagg ccgttctcat gataaccgac cacgcgcccg cgcagtttcg
       61 aaaggctctg atcgaggttc agcgcgcagg cgacggcggg cgcgccgagc agcagcagcg
      121 ccttcggccg cgccagccgg atcagcgcgt gcaggaacgg cgcgctcagg gccacgtcct
      181 ccggcgtgag cgggcgcccg cccgccggac gccacggaac gcacggcgcc agcaggcttt
      241 gcgccgccac accggcggcg tgcagcgcct tgtccagcat ctcgcccggc ttgcccgcga
      301 aggcgaggcc ggtttcgtcc tcgtccgcgt cgggcggctc gccgatcacc atcagggccg
      361 gggcggggac gccgcgaaac ctcaccagcg acttcgcgcc ttcgtggcgc aacggcatgt
      421 gctggaacgc ctcaagctcg gcgtaaagct cgtcgaagtc gcgcgcggcg tgggcgcgct
      481 gcttcgcctc atgcgccgcc gcgccgaggt cgagatgggc caggctttgc gggcgcagcg
      541 cgttggcggg cgccgccgcc gggccgggcg tcacgtgaat ttgcgcggcc gccgcggggg
      601 cgcgttgaat ccgttgttta ttttcgctca gagtccgatt aagaggctct gtttcatagg
      661 cgtcgtccag ctcatgatcg atcagaaagc ccagatagct ttcgatctcc tgatggatgt
      721 catcgctgaa cggcatagga tttacggacg gcctgttcgt ctagagcttc gttggcctgt
      781 cataaggcga cttgcggcga agttcaaagc aaaacggtcg ggaaagagca agggcatggc
      841 tgacacgcag gacgcggttg cgcgcgaatc gatggattac gacgtcgtca tcgtcggcgg
      901 cggcccggcg gggctgtcgg cggccatccg cctgaagcag aaggcggcgg aggccgggac
      961 cgaggtcagc gtcgccgtgc tggagaaagg ctccgagatc ggcgcgcaca tcctgtccgg
     1021 ggcggtgatc gatccgtcgg ggctggcgaa gctgtttccc gactggcagg cgcgcggcgc
     1081 gccgctggag acgccggtga cggacgaccg cttcctgttc ctggggccgc agggggtgat
     1141 ggacatctcc tggctgccca agcccggcta tatgagcaat cacggctgct acatcgcctc
     1201 gctcggcaat ctgagccgct ggctggccga gcaggccgag gggctgggcg tcgaaatcta
     1261 tccgggcatg gcctgctccg aagtgcttta cgacgcggac ggcggcgtgg cgggcgtggt
     1321 cgcgggcgtg ttcggcgtcg ccagggacgg ccatcacaag ccggactatc agccgggcct
     1381 ggaactgcgc gccagataca ccttcttcgc cgagggcgcg cgcggctcgc tgtcgcagca
     1441 ggtcatccgc cgcttcgagc tggacaagga cagcgactgt cagaagtacg gcctcggcgt
     1501 caaggagctg tggaaggtcc ggccggagca attccggccc ggcctcgtgc agcacacgct
     1561 gggctggccg ctcgacgacc gcaccggcgg cggctcgttc ctgtaccatt tc
//
LOCUS       DASVLL010000342         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_291107,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000342 DASVLL010000000
VERSION     DASVLL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291107"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VF402_05680"
     CDS             <1..1355
                     /locus_tag="VF402_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX7799820.1"
                     /translation="KAKSFKHIELPAATRYAAEDADVTLRLWRVLKPRLAREGLLGVY
                     ETLERAMPSVLSRMELAGIRIDPQVLQSLSHDFGVRMNEIEAEAHNLAGRPFNLNSPQ
                     QLGQIFFDEMALPGGKRTASGQWATDVKVLEDLAEKGQPLAKLLLDYRQMAKLKGTYT
                     DNLTGHADPKTQRIHTSFHLAATPTGRLSSNEPNLQNIPIRTAEGRKIRRAFIAKPGH
                     RLISADYSQIELRLLAHIGDIPQLKAAFARGDDIHAMTASEMFAVPMAEMTPEIRRRA
                     KAINFGIIYGISAFGLANQLGIERGEAGRYIETYFERFPGIKSYMDATKAFVAANGYV
                     ATLMGRKIHIPEINGKGAQKAFAERAAINAPIQGTAADIIRRAMMRMPLALRDAGFAT
                     AMLLQVHDELIFEAPEAEVEAVMPLIRDVMARAAEPAYPISVPLVVEAHAADNWDEAH
                     "
     gene            1357..1752
                     /locus_tag="VF402_05685"
     CDS             1357..1752
                     /locus_tag="VF402_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7799821.1"
                     /translation="MTALSLDHINIQTRDMARTIAFYRDLLGLEAKGAPRRDPAERQW
                     LYAGGRAVIHLNLFGTGNTVARDVQPGAATGAIHHVAFECDDFDACLARIEAMGLDHG
                     FADLPEIGLKQLFVTDPDNVLLELNFRRA"
     gene            complement(1739..>2235)
                     /locus_tag="VF402_05690"
     CDS             complement(1739..>2235)
                     /locus_tag="VF402_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX7799822.1"
                     /translation="SDFGSYWRGMLRMAFSFTLGLWLYRVHDRFPKLRPGWIVLSLVL
                     IVAMCVPRIPRAVAYGNGLLEVAMVIFVFPLIVLLGAHSRIGRVEMAACKIAGHISYP
                     LYILHYPFLLIYMNYVNFRHPAMPDRLLWAGVAFIVVVGCAWAALKLYDEPIRRALKP
                     LTRA"
BASE COUNT          442 a          753 c          709 g          331 t
ORIGIN      
        1 agaaggccaa gtcgttcaag catatcgaac tgcccgccgc cacgcgctac gccgccgagg
       61 acgccgacgt gaccttgcgg ctgtggcggg tgttgaagcc gcgtctggcg cgcgaggggc
      121 tgctcggcgt ttacgagacg ctggagcgcg ccatgccgtc cgtgctgtcg cgcatggagc
      181 tggccggcat ccgcatcgac ccgcaggtcc tgcaatcgct gtcgcacgat ttcggcgtgc
      241 gcatgaacga gatcgaggcc gaggcgcaca accttgcggg ccgtccgttc aacctcaaca
      301 gcccgcagca actgggccag atcttcttcg acgaaatggc cctgcccggc ggcaagcgca
      361 ccgcgtcggg ccagtgggcg acggatgtga aagtgcttga ggatctggcc gaaaagggcc
      421 agccgctcgc caaactgctg ctcgactacc gccagatggc caagctgaaa ggcacctata
      481 ccgacaatct gaccggccac gccgacccga agacccagcg catccacacc agcttccatc
      541 tggccgcgac gccgaccggg cgcctgtcgt ccaacgagcc gaacctgcaa aacatcccga
      601 tccgcacggc ggaagggcgc aagatccggc gcgccttcat cgccaaaccg ggccacaggc
      661 tgatcagcgc cgactattcg cagatcgagc tgcggctgct ggcccatatc ggcgacattc
      721 cgcaactgaa ggccgcgttc gcgcgcggcg acgacatcca cgccatgacg gcctcggaaa
      781 tgttcgccgt gccgatggcc gagatgaccc cggaaatccg ccgccgcgcc aaggcgatca
      841 atttcggcat catctacggc atttccgcct tcggactggc caaccagctc ggcatcgaac
      901 gcggcgaggc cgggcgctat atcgaaacct atttcgagcg cttccccggc atcaagagct
      961 atatggacgc caccaaggcg ttcgtggccg ccaatggcta tgtcgccaca ttgatgggcc
     1021 ggaaaatcca tattcccgaa atcaacggca agggggcgca gaaggcgttc gcggagcgcg
     1081 ccgccatcaa cgcgccgatt caggggaccg ccgccgacat catccgccgc gccatgatgc
     1141 gcatgccgct ggcgctgcgc gacgcgggtt tcgcgaccgc catgctgttg caggtgcatg
     1201 acgaactcat cttcgaggcg cccgaagccg aggtcgaggc ggtcatgccg ctgattcgcg
     1261 acgtgatggc cagggccgcc gaacccgcct atccgatcag cgtgccgctg gtcgtcgaag
     1321 ctcacgccgc cgacaactgg gatgaggccc attgacatga ccgctctgtc tctcgaccat
     1381 atcaacatcc agacgcgcga catggcgcgg accatcgcct tttaccgcga cctgctgggc
     1441 cttgaggcga agggcgcgcc ccggcgcgat ccggccgagc ggcaatggct ctatgcgggc
     1501 gggcgcgcgg tcatccacct caacctgttc ggcacgggca acaccgtcgc ccgcgacgtg
     1561 cagcccggcg cggccaccgg cgcgatccac catgtcgcct tcgagtgcga cgatttcgac
     1621 gcctgcctgg cgaggatcga ggcgatgggg ctggaccacg gtttcgccga cctgccggag
     1681 atcgggttga aacaactgtt cgtcaccgat cccgacaatg tgctgctgga gctgaacttc
     1741 aggcgcgcgt gagcggcttt aaggcgcgcc ggatcggctc gtcgtaaagc ttcagcgccg
     1801 cccaggcgca gccgaccacc acgatgaagg cgacgccggc ccacaggagc cggtccggca
     1861 tggccggatg gcggaagttg acatagttca tgtagatgag caggaacgga tagtgcagga
     1921 tatagagcgg gtaggagata tggcccgcga tcttgcacgc cgccatctcg acgcggccga
     1981 tccgcgaatg tgcgcccagc agcacgatca gcggaaagac gaagatcacc atcgcaacct
     2041 ccagaagccc gttgccgtac gccacggcgc gcgggatgcg cggcacgcac atggcgacga
     2101 tcaggacaag gctgagcacg atccagccgg ggcgcagctt cggaaagcga tcatgcacgc
     2161 ggtagagcca caggcccagc gtgaacgaaa acgccatgcg cagcatgccg cgccagtagc
     2221 tgccgaagtc cgagc
//
LOCUS       DASVLL010000343         4857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_130115,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000343 DASVLL010000000
VERSION     DASVLL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130115"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VF402_05695"
     CDS             <1..1067
                     /locus_tag="VF402_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX7799823.1"
                     /translation="MSLYALDDVERLTRRGGEAGRARPSGPVLSGGAVTRGEAGIDTE
                     ISITLDGRHYYRGKDSLKLAGRENFEAVAALLWQSPTPNPFGPLKPRPDVNFPGGPRT
                     RVLSMLSRRMEEDALADVDPKRDLRLEAASTLNEMVDAVTNGGPRLFFHQRLARAWKV
                     YDMADIDLIRRALVLCADTTLDEATLAARVAAATLGPLAGPVMAGFAALMGPRLGGRI
                     SRAESYVTQVRRHGNPHALAKSWLDRGLELPGFEKPASPSEQERAHNLIEAAPQMGAD
                     LKNVLTVGEDLTGQGAGLSLALALVGRHLDLPREAPLTLYGIGRSAGWLAHAIEQMQA
                     GAAPKARLRYIGDYPDAEAQ"
     gene            1126..1926
                     /locus_tag="VF402_05700"
     CDS             1126..1926
                     /locus_tag="VF402_05700"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7799824.1"
                     /translation="MHNLLAALLGLIEGLTEFLPVSSTAHLLIAEKLLRFSDPGGNFT
                     VMIQLGAILAVVVLYFRRLWQAVIDLPGKAEARRFALSVIIAFLPAAVIGVIIHKLIG
                     EMFENLTIICVALIVGGLALWLVDKFAPKPEYHDSMQLNFRTALLIGLFQCLAMIPGM
                     SRSGSTLIGGMLSRVDKKAGAEFSFFLAIPTMLGAFTLDFVKSYKSLTSDQYGAIAIG
                     FVVAFLAALVVIKPFVGFIQKRGYGFFALWRVLVGGAGLFLIYGLHAL"
     gene            complement(1949..3268)
                     /locus_tag="VF402_05705"
     CDS             complement(1949..3268)
                     /locus_tag="VF402_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7799825.1"
                     /translation="MKSFICVAALALAVAGGAHAADWPMFMADAHRTGAAADPAPPPE
                     AMRGLHAAWSLDLPTSISASPVAAGGLIYIAGDNGDLYAFDLATRKRLWLYHAEGGLA
                     STPAVAGGRLFVLSRDGYVHALDARTGALIWRFATGGEARYAQVGGYGVAKAMGPVPD
                     PWDFWLSSPLVQDGKVYVGSSDGHMYALDANDGAKLWAYDAGAPIHGAPALSGGRLAF
                     GTLDTQVIALDAATGTKLWSFKGGVDPQYGILVGVPAAPTFDGDALYIGTRDAHLYAF
                     DAATGKPRWTYDAKGSWVLTTAAVDASSLYFATSDTGLFVAADKATGAERYRVTTGVW
                     TYASPVLIGGRYVAFGTMDGVLEVIDKATGAPVWRWQSPEGQADADRLIGADGKFDSK
                     ILFAPDQQVQGAVEKVKALGAFVASPIWVDGQLIAADANGHVRAFAP"
     gene            complement(3307..4281)
                     /locus_tag="VF402_05710"
     CDS             complement(3307..4281)
                     /locus_tag="VF402_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX7799826.1"
                     /translation="MVQLVTVFGGSGFVGKQVVRALAKKGWRVRVAVRRPTIAYDLKP
                     NGDVGQIQVVRCDVRKSDDIAAALHGADAVVNLVGILMQGGGQTFEGLHHKAAEAIAQ
                     AAAAKGVRAFVQMSAIGADPKSASAYAASKGRGEQAVRKAIPTATIVRPSIIFGPQDG
                     FFTALAQQIKAMPFMPAIGGGKTKFQPVYVGDVAQAIANALGDEAAYGQTYELGGPEV
                     FTFSQLVSYTAEEIMTPRAQVWTPFGAAALIGVFGDILAGLHGVLPLLVPAPPLTGDQ
                     VVLLRTDNVVADGAKGLKDLGVAATAIESILPTYLWRFRKNGQFAVAA"
     gene            4503..4589
                     /locus_tag="VF402_05715"
     tRNA            4503..4589
                     /locus_tag="VF402_05715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4537..4539,aa:Leu,seq:cag)
BASE COUNT          774 a         1709 c         1597 g          777 t
ORIGIN      
        1 gcatgagcct ctacgcgctc gacgacgtcg agcggctgac gcggcgcggc ggcgaggcgg
       61 gccgcgcgcg gccgtccggc ccggtcctga gcggcggcgc cgtgacgcgc ggcgaggcgg
      121 gcatcgacac cgagatctcg atcacgctgg acgggcggca ctattatcgc ggcaaggaca
      181 gcctgaaact ggccgggcgc gagaatttcg aggccgtcgc cgccctgttg tggcaaagcc
      241 cgacgccgaa cccgttcgga ccgctcaagc cgcgcccgga cgtcaacttc cccggcgggc
      301 cgcgcacgcg cgtgctgtcc atgctgagcc gccgcatgga ggaggacgcg ctggccgacg
      361 tcgatccgaa gcgcgacctg cggctggagg cggccagcac gctgaacgag atggtcgacg
      421 ccgtcaccaa cggcgggccg cgcctgtttt tccaccaacg gctggcgcgc gcgtggaagg
      481 tgtacgacat ggccgacatc gacctgatcc ggcgcgcgct ggtgctgtgc gccgacacca
      541 cgctggacga ggccaccctg gccgcgcgcg tcgccgccgc gaccctgggg ccgctggccg
      601 ggccggtcat ggcgggcttc gccgccctga tggggccgcg tctgggcgga cgcatctcgc
      661 gcgccgaatc ctatgtcacg caggtgcgcc gccacggcaa tccgcatgcg ctggccaagt
      721 cctggctcga ccgcgggctg gaattgcccg gcttcgaaaa gcccgcctcg ccgtccgagc
      781 aggagcgcgc gcacaacctg atcgaggccg cgccgcagat gggcgccgac ctgaaaaacg
      841 tgctgacggt cggcgaggat ctgaccgggc agggcgcggg cctgtcgctg gcgctggccc
      901 tggtcgggcg tcatctcgac ctgccgcgcg aggcgccgct gaccctctac ggcatcggcc
      961 gcagcgcggg ctggctggcc cacgccatcg agcagatgca ggcgggcgcg gccccgaagg
     1021 cgcgcctgcg ctatatcggc gattatcccg acgccgaggc gcaatagcca cttgccgccg
     1081 cgtgaaaacg ccgtatgaga tggctgtcca tcttaggggt tttccatgca caacctgctt
     1141 gcggccttgc tcggcctgat cgagggcctg accgaatttc tgccggtgtc gtcgaccgcg
     1201 catctgctga tcgccgagaa actgctgcga ttttccgatc ccggcggcaa tttcaccgtc
     1261 atgatccagt tgggcgccat tctggccgtg gtcgtgctgt atttccgccg cctgtggcag
     1321 gcggtgatcg acctgccagg caaggccgag gcgcgccgct tcgcgctcag cgtcatcatc
     1381 gccttcctgc ccgccgccgt catcggggtg atcatccaca agctgatcgg cgagatgttc
     1441 gagaacctga cgatcatctg cgtcgccctg atcgtcggcg ggctggcgct gtggctggtc
     1501 gacaagttcg cgccgaagcc ggaatatcac gactcgatgc agttgaattt caggaccgcg
     1561 ctgctgatcg gcctgtttca gtgcctggcc atgatccccg gcatgtcgcg ctccggctcg
     1621 accctgatcg gcggcatgct gagccgcgtc gacaagaagg ccggggcgga gttttccttc
     1681 ttcctcgcca tcccgaccat gctcggcgcc ttcacgctcg acttcgtcaa gagctataag
     1741 tcgctgacct ccgatcagta cggcgccatc gccatcggct tcgtggtcgc cttcctggcg
     1801 gcgctggtcg tgatcaagcc gttcgtcggc ttcatccaga agcgcggcta cggcttcttc
     1861 gcgctgtggc gcgtgctcgt cggcggcgcg ggcctgttcc tgatctacgg cctgcacgcg
     1921 ctctgaggcg ggtcggaagc gcggaagatc agggcgcgaa cgcccgcaca tgcccgttgg
     1981 cgtcggcggc gatcaactga ccgtcgaccc agatcggcga ggcgacgaag gcgcccagcg
     2041 ccttgacctt ctcgaccgcg ccctgcacct gctggtccgg cgcgaacagg atcttggagt
     2101 cgaacttgcc gtcggcgccg atcagacggt cggcgtccgc ctgcccttcc gggctttgcc
     2161 agcgccagac cggcgccccg gtcgccttgt cgatcacctc cagcacgccg tccatcgtcc
     2221 cgaaggcgac gtagcgcccg ccgatcagga ccggcgaggc gtaggtccac acgccggtcg
     2281 tcacccggta acgctccgcg cccgtcgcct tgtccgccgc cacgaacagg ccggtgtcgg
     2341 aggtggcgaa atagaggctg ctcgcatcga ccgccgccgt ggtcagcacc cagcttccct
     2401 tggcgtcgta ggtccagcgc ggcttgcccg tcgccgcgtc gaaggcgtag aggtgcgcgt
     2461 cgcgcgtccc gatatagagc gcgtcgccgt cgaaggtcgg cgccgccgga accccgacca
     2521 gaatgccgta ttgcggatcg accccgccct tgaacgacca cagcttcgtc cccgtcgcgg
     2581 cgtcgagcgc gatcacctgc gtgtccagcg tgccgaaggc cagccgcccg ccgctcagcg
     2641 ccggggcgcc gtggatcggc gcgcccgcgt cataggccca cagtttcgcg ccgtcgttcg
     2701 cgtcgagcgc gtacatatgt ccgtcgctgg agccgacata gaccttgccg tcctgaacca
     2761 gcggcgagga cagccagaaa tcccacggat cgggcaccgg ccccatcgcc ttggccacgc
     2821 cgtagccgcc gacctgcgcg taccgcgcct cgccgccggt ggcgaagcgc cagatcagcg
     2881 cgccggtcct ggcgtcgagc gcatggacat agccgtcgcg gctcagcacg aacagccgcc
     2941 cgccggccac cgccggagtc gaggccagcc cgccctcggc gtggtagagc cacagacgct
     3001 tgcgcgtcgc cagatcgaag gcgtagaggt cgccgttgtc gcccgcgata tagatgaggc
     3061 cgcccgccgc gaccggcgag gcgctgatcg aggtcggcag gtcgaggctc cacgccgcat
     3121 gcaggccgcg catcgcctcc ggcggcggcg cgggatcggc ggcggcgccg gtgcgatgcg
     3181 cgtccgccat gaacatcggc cagtccgccg catgggcgcc gcccgccacc gccagcgcaa
     3241 gcgccgcgac acagataaag gacttcacat ctcccccctc gccgctttgg cgcggcttgt
     3301 tgcgaatcag gccgcgaccg cgaactggcc gttcttgcgg aagcgccaca gataggtcgg
     3361 caggatcgat tcgatcgccg tggcggcgac gccgaggtct ttcagcccct tggcgccgtc
     3421 ggcgacgaca ttgtccgtcc tgagcagcac gacctgatcg ccggtcagcg gcggcgccgg
     3481 aaccagcagc ggcagcacgc cgtgcagccc ggccagaata tcgccgaaca cgccgatcag
     3541 cgcggcggcg ccgaacggcg tccacacctg cgcgcgcggc gtcatgattt cctcggccgt
     3601 gtagctgacc agctggctga aggtgaagac ctccggcccg cccagctcgt aggtctggcc
     3661 gtaggcggct tcgtcgccca gcgcgttcgc gatggcctgc gccacgtcgc cgacatagac
     3721 cggctggaac ttcgtcttgc cgccgccgat ggcgggcatg aagggcatgg ccttgatctg
     3781 ctgcgccagc gcggtgaaga agccgtcctg cggtccgaaa atgatgctgg ggcgcacgat
     3841 ggtcgccgtc gggatcgcct tgcgcacggc ctgttcgccg cggcccttgc tggccgcgta
     3901 ggccgaggcc gacttcggat cggcgccgat ggccgacatc tgcacgaagg cgcggacgcc
     3961 cttcgcggcg gcggcctgcg cgatggcttc ggcggccttg tggtgcaggc cctcgaaggt
     4021 ctgacccccg ccctgcatca gaatgccgac cagattgacg accgcgtcgg cgccgtgcag
     4081 cgccgccgcg atgtcatcgg atttacgcac gtcgcagcgc acgacctgaa tctggccgac
     4141 gtcgccgttc ggcttcagat cgtaggcgat ggtcggcctg cgcaccgcga cgcgcacgcg
     4201 ccagcccttt ttcgccagcg cccgcaccac ctgcttgccg acaaaacccg aaccgccgaa
     4261 aaccgtgacc agctgcacca tgcgcgatat cctgaaaata agcactcgcc gcgccggacc
     4321 gtcaggcagg cgccgcctta tatcgcctcg ataaaccaag cctccggctc aggcaaccgg
     4381 ttaggcgggc gcgtccgaaa cttttgtgaa tagccgcgca aaaacccgtt gaccgcgaaa
     4441 aactttcccc ctataggtcg ccgcctcgcc ggaaacggcg ggaacgacca cctaagagac
     4501 gtgcccaggt ggcggaattg gtagacgcgc tggcttcagg tgccagtggc ttaacggccg
     4561 tgaaggttcg agtcctttcc tgggcaccat ctagggttat acccctgata acattgattt
     4621 tcctcgcttt ttcggcactg ctggtacaca aaaccggtac acacgaggac gatctcaggc
     4681 atgaaaaact cccaatacct gcactgcctt aaaggccgct actactgccg tgttcgctgg
     4741 ccgaaagagg tctggaagac caagggaacg ggggccttca agcgctcgtt gggaacgtcc
     4801 agccgagacg aagctgtcag aagattgcct gctgccctgc aagaatacca aaacgaa
//
LOCUS       DASVLL010000344         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_452710,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000344 DASVLL010000000
VERSION     DASVLL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452710"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..140)
                     /locus_tag="VF402_05720"
     tRNA            complement(66..140)
                     /locus_tag="VF402_05720"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Glu,seq:ttc)
     gene            complement(234..1121)
                     /gene="dapD"
                     /locus_tag="VF402_05725"
     CDS             complement(234..1121)
                     /gene="dapD"
                     /locus_tag="VF402_05725"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX7799827.1"
                     /translation="MSDLAAFKEDIEAAWEARETLSPGYAGKYREAVDKVLGLLDNGR
                     FRVAEKKDGPNGSTWITHDWLKKAVLLSFRLNANALMHTGRGLFEGSPIGPFWDKVPN
                     KFARWSDKDFQEAGFRAVPGAIVRAGAFVGKNVVLMPSFVNIGAYVDEGTMVDTWATV
                     GSCAQVGKNVHLSGGVGIGGVLEPLQAAPTIIEDNCFIGARSEVVEGCIVREGSVLGM
                     GVYIGKSTRIVDRATGEIFYGEVPPYSVVVAGSMPSSNASVSAGANTPAPNLYCAVIV
                     KRVDAQTRSKTGVNELLRD"
     gene            complement(1181..2032)
                     /locus_tag="VF402_05730"
     CDS             complement(1181..2032)
                     /locus_tag="VF402_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HEX7799828.1"
                     /translation="MRGPVFPAIFLAAAMLSLALPAAAQESQQPHAVRDSGGAWSGAA
                     VKPYLSPRDTAAFAKQIEADLAAKGAHVAIVFRAGRPRATMPKGISYTHAAFWVYGDI
                     EGGDGRVYKGYSVYNLYQGDGRDLPRDKSYLKQDFPVDFVGGSQADDVGVIIPSPEMQ
                     RRIFDIIESPAYERLHVADYSVIANPLDARYQNCTEFVLDVIAAAAWNTDDYAQIKAD
                     LKAHFQPTVVRVNGLERLFGPMVDSAVKTGDQSGPLVTATYESVAAFMRGNGLMAQTY
                     RLDRNKP"
     gene            complement(2032..2409)
                     /locus_tag="VF402_05735"
     CDS             complement(2032..2409)
                     /locus_tag="VF402_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799829.1"
                     /translation="MKPSLTGICVAAALMAATSAATAAHAQDSLANASKASADSAVAL
                     GEMTEAGVKVVGGAVALPVAAVGVVMEGAGKALHQGGKAMWDSANGPLEITPETVTAQ
                     PAPQVPYDRPADAPYDAPAKAGH"
     gene            complement(2490..>2901)
                     /locus_tag="VF402_05740"
     CDS             complement(2490..>2901)
                     /locus_tag="VF402_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7799830.1"
                     /translation="EVHDVSIDKLAPDPELNGLIADLPGRKLVFTNGDERHATRILAR
                     LEMTALFDGVFHLEHANFIPKPNLVTFERMMAAHAVAADRAAFFEDSPRNLKPAFELG
                     MKTILVGPKAVGNDDPFIDFRGPDLKTFLSRQEM"
BASE COUNT          474 a          925 c          994 g          508 t
ORIGIN      
        1 tggcgcagaa gatctacgac ttctttcacg gggaatgagg cgcctgaaaa cgaggcgcgc
       61 gccgctggca gtccctaggg gagtcgaacc cctcttttca ggttgaaaac ctgacgtcct
      121 aaccgataga cgaagggacc gcgctggcga accggaccgg gacagcgtcc tgaccggagg
      181 cgcgcttttg gacgattgcg cgccgccggt caagaggctt tcgcggaaaa acatcagtcg
      241 cgcagcagtt cgttgacgcc ggtcttggag cgggtctgcg cgtcgacgcg cttgacgatg
      301 acggcgcagt agaggttggg cgcgggggtg ttcgcgccgg cgctgacgct ggcgttcgag
      361 gacggcatgg agccggccac gaccaccgaa tagggcggca cctcgccgta gaagatttcg
      421 cccgtggcgc ggtcgacgat gcgggtggat ttgccgatat agacgcccat gcccagcacc
      481 gagccttcgc gcacgatgca gccctcgacc acctcggagc gcgcgccgat gaagcagttg
      541 tcctcgatga tggtcggcgc ggcctgcaac ggctccagca cgccgccgat gccgacgccg
      601 cccgacaggt ggacgttctt gcccacttgc gcgcaggagc cgaccgtggc ccaggtgtcg
      661 accatcgtgc cttcgtcgac ataggcgccg atattgacga aggacggcat cagcacgacg
      721 ttcttgccga cgaaggcccc ggcgcgcacg atggcgcccg gcacggcgcg gaagcccgcc
      781 tcctgaaagt ccttgtcgct ccagcgcgcg aacttgttgg gaaccttgtc ccagaacggc
      841 ccgatgggcg agccttcgaa caggccgcgc ccggtgtgca tcagcgcgtt ggcgttcagg
      901 cggaaggaca gaagcacggc ctttttcagc cagtcgtgcg tgatccatgt cgagccattg
      961 gggccatcct tcttttccgc cacgcggaag cggccgttgt ccagcaggcc cagcaccttg
     1021 tccaccgcct cgcggtactt gccggcatag ccgggcgaca gggtttcgcg cgcctcccag
     1081 gcggcttcga tatcttcctt gaaggcggcg aggtcggaca tggcgggcgc tctctgaagt
     1141 gctgatctgg cccccgcctt aaaaggcccg ccgccgccga tcaaggctta tttcggtcga
     1201 ggcgataagt ttgcgccatc aggccgttgc cgcgcatgaa ggccgccacg ctttcatagg
     1261 tcgcggtcac gagcgggccg gactggtcgc cggtcttgac cgctgagtcc accatcgggc
     1321 cgaacaggcg ctcaaggccg ttgacgcgga ccacggtggg ctggaaatgc gccttcaggt
     1381 cggccttgat ctgggcgtag tcgtcggtgt tccacgccgc cgccgcgatc acgtccagca
     1441 cgaattcggt gcagttctga tagcgcgcgt cgagcggatt ggcgatcacc gaatagtcgg
     1501 cgacgtgcag acgttcatag gccggcgatt cgatgatgtc gaagatgcgg cgctgcattt
     1561 ccggcgaggg tatgatcacg ccgacgtcgt cggcctggct accgccgacg aaatcgacgg
     1621 ggaagtcctg cttgaggtag gacttgtcgc gcggcaggtc gcgaccgtcg ccctgataga
     1681 ggttatagac cgaatagccc ttatagacgc ggccgtcgcc gccctcgatg tcgccataga
     1741 cccagaaggc ggcgtgggtg tagctgatcc ccttcggcat cgtcgcgcgc gggcgtccgg
     1801 cgcggaagac gatggcgacg tgcgcgccct tcgcggccag atcggcctcg atctgcttgg
     1861 cgaacgcggc cgtgtcgcgc ggcgacagat agggtttgac cgccgcgccg ctccaggcgc
     1921 cgccggaatc gcgcaccgcg tggggctgct gcgactcctg cgcggcggcg ggcagggcga
     1981 ggctcagcat cgccgccgcc agaaaaatcg ctggaaaaac cgggccccgc atcagtggcc
     2041 ggccttggcg ggcgcatcat atggcgcgtc ggcggggcgg tcgtagggaa cctgcggcgc
     2101 cggttgcgcg gtgacggttt ccggcgtgat ctccagcggc ccgttggccg agtcccacat
     2161 cgccttgccg ccctgatgca gcgccttgcc cgcgccctcc atcacgacgc cgacggcggc
     2221 cacgggcagg gcgaccgcgc cgccgaccac cttgacgccc gcctcggtca tctcgcccag
     2281 cgcgaccgcc gaatccgccg acgccttcga ggcgttggcc aggctgtcct gagcatgggc
     2341 cgcagttgcg gccgaggttg cggccatcag ggccgccgct acgcagatac cggtcagaga
     2401 gggtttcatc gcgcgctcct ttgctgtgaa cgatgtaatg tgaacatcgt tcaatattgc
     2461 gcgcgcgtca agctaaattt gaacgacgtt cacatttctt ggcggctcag gaaggtcttg
     2521 aggtcggggc cgcggaaatc gatgaacgga tcgtcgttgc cgacggcttt cggcccgacc
     2581 agaatcgtct tcatgcccag ctcgaaggcc ggtttcagat ttcgcgggct gtcctcgaag
     2641 aaggcggccc tgtcggcggc gaccgcgtgg gcggccatca tgcgctcgaa ggtgacgagg
     2701 ttgggcttgg ggatgaagtt ggcgtgttcg agatggaaaa cgccgtcgaa cagcgcggtc
     2761 atctccagcc tggccaggat gcgcgtggcg tggcgttcgt cgccgttggt gaagaccagc
     2821 ttgcggcccg gcaggtcggc gatcagcccg ttcagctccg ggtcgggcgc cagcttgtcg
     2881 atggagacgt catgcacctc g
//
LOCUS       DASVLL010000345         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_183375,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000345 DASVLL010000000
VERSION     DASVLL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183375"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VF402_05745"
     CDS             complement(<1..705)
                     /locus_tag="VF402_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7799831.1"
                     /translation="MLNGSRTSHAGEGRDAAPGLRLGALAWHFGWSGFNLLGVIGLML
                     MRASPEGVASPLVMAIPGAACVLLLWRDTRLTRRALIWIWAVCAGLAVWLSGGIAGPQ
                     AMWVAAPMLAAVVLNQRSLISLGAALAFVVALLGGFVSLWGGEIAFSHGERLVLSLLS
                     VFSTVIGLGVALLPALRARFERAAGAEDAREKLFHMLTEQPALIVCLNESGRLTAGYG
                     EAPAGVDLRLMMQLGVG"
     gene            902..1672
                     /locus_tag="VF402_05750"
     CDS             902..1672
                     /locus_tag="VF402_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7799832.1"
                     /translation="MAFFQRPDAGDGGSKTWTSEAAPPAADRDAARRAFLRMVSHELR
                     TPLNSIIGFADILHGQLYGPLGSPEYVEYAGIIRDSGARLLHLFNNVLELIRLEGGGG
                     FSPAPEPVQPWLDEAVTRRRAAAEARGVALRLRPSGEKLSAAFDPKGLLTCLDHLIGN
                     AVDFTAAGDAVEISARRDGAYALISVYNRGPAPAPDDIERLMNPFEQGGDALSRSHEG
                     AGLGWPIVRLTAQAMGGEFRVATRAGEALKAVLRFPAA"
     gene            1764..3266
                     /locus_tag="VF402_05755"
     CDS             1764..3266
                     /locus_tag="VF402_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799833.1"
                     /translation="MRHRLLLACAALALIATPALAQMGGGMGGGGGRGGHRGGGGGGG
                     DASDGKGDQPTVPTRPAPQQLPVAMRLGGVIDIIGESKAAEGGSVASARPDLSAIFVS
                     RGGALTLKDVSLSTSGAVSLVDDSRGSGLDSALLINESGQAAMTGGRISTSGLGANAA
                     YVAGPGARLNLQGVVIATSSSNAYGVDAVDGAALGAANLTISTMGDHAPALASRRALP
                     MQLSDLNLHTAASGSPLLLAEGDMTVTNAKGQAERDDGLAVAGAHRVNLTTPDLQADG
                     YAISVAAGSGGARPGGGPQSGAMPGPSLAQDDAPDDPDAGKVKPITSLLSAPPDDQRG
                     ADIQVTGGALRGGRAVLSVSNAHAHIVLTGVSLSSESGVILRAAAGQAGTVGRNGGDA
                     DLELHGETVAGDFATDVISSIRVSLLDQSHLTGKATANTDVTLDAGSDWTLSDDSHVG
                     KLVVTDLDKPDAIANIRSGGHTLYYDPHRNPWLDHKTWPLPGGGQLTPDI"
     gene            complement(3290..>4121)
                     /locus_tag="VF402_05760"
     CDS             complement(3290..>4121)
                     /locus_tag="VF402_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX7799834.1"
                     /translation="GLYALTLFEAATPVDDLPWFMDYTLYLDRKGYPAPGPALKRDGS
                     SLGEIKDKPCALIRWLPGRWPRNPDARHAASAGEYLAKLHLTGGDFPRFRENAMGPKA
                     WPGLIERCEPKAETSPRALDILADFRAELAFLRANWPDDLPRGAIHADYFTDNVLMSE
                     DGQVTGVIDYYYACTDFFAYDLAVALNAWGFTPGGRAQPEMMAAFAGAYQATRPLSDA
                     EIAALPLFARGAAVRFTLTRLYDLLNHDPSWVVKPKDPEAFYRRLDYHRGVKDGRAYF
                     "
BASE COUNT          685 a         1565 c         1339 g          532 t
ORIGIN      
        1 gccgacgccc agttgcatca tcaggcgcag atcgaccccc gccggcgcct cgccatagcc
       61 cgccgtcagc cgcccgctct cgttgaggca gacgatcagc gccggttgct cggtcagcat
      121 gtggaacagc ttttcgcgcg cgtcctcggc cccggcggcg cgctcgaagc gcgcgcgcaa
      181 ggcgggcagc agcgccacgc ccagcccgat caccgtcgaa aacaccgaca gcagcgacag
      241 gaccagccgc tcgccgtgcg aaaaggcgat ctcgccgccc cacaggctga cgaaaccgcc
      301 cagcagcgcg acgacgaagg ccagcgccgc gcccagcgag atcagcgagc gctggttcag
      361 caccacggcg gccagcatcg gcgccgcgac ccacatcgcc tgcggcccgg cgatcccgcc
      421 gctcagccac accgccagcc cggcgcagac cgcccagatc cagatcagcg cccgccgcgt
      481 cagccgcgtg tcgcgccaca aaagcagcac gcacgccgcc cccggaatcg ccatgaccag
      541 cggcgaggcc acgccctccg gcgacgcccg catcagcatc agcccgatca cgcccagcag
      601 gttgaatccc gaccagccga aatgccaggc cagcgcgccc aggcgcaggc ccggcgcggc
      661 gtcgcgccct tccccggcgt gcgatgtgcg gcttccgttc agcaagtcga tcctttcagg
      721 cgtcgcgcga cgctcaccat aggcgcaatt ctatcggtcc gccgctaaaa tacgaagagt
      781 ctcgcgccat aagcgagtac aaatcgcacg cgcgggtaag gcaaccttaa agatcggcgc
      841 cgtaaggtcg gcgcaaaggg cgagcgcgac cggctcaaac gccctctcag ggttaaggta
      901 tatggctttt ttccagaggc cggacgccgg cgacggcggc tcgaaaacct ggacgtccga
      961 ggcggcgccc cccgccgccg accgcgacgc cgcgcgccgc gccttcctgc gcatggtcag
     1021 ccatgagctg cgcaccccgc tcaacagcat catcggtttc gccgacatcc tgcacggcca
     1081 gctttacggc ccgctcggct cgcccgaata cgtcgaatac gccggcatca tccgcgacag
     1141 cggcgcgcgg ctgctgcacc tgttcaacaa cgtgctggag ttgatccggc tcgaaggcgg
     1201 cggcggcttc tcacccgcgc cggaaccggt gcagccatgg ctggacgaag ccgtgacgcg
     1261 tcgccgcgcc gccgccgagg cgcgcggggt cgctttgcgc ctgcgcccgt ccggcgaaaa
     1321 actgtcggcg gcgttcgacc ccaaggggct gctgacctgt ctcgaccacc tgatcggcaa
     1381 cgccgtcgac ttcaccgccg ccggcgacgc ggtcgagatt tccgcccgcc gcgacggcgc
     1441 ctatgcgctg atcagcgtct ataatcgcgg ccccgccccg gcgcccgacg acatcgaacg
     1501 gctgatgaat ccgtttgaac aaggcggcga cgcgctgagc cgcagccacg aaggcgccgg
     1561 actcggctgg ccgatcgtgc gcctgaccgc gcaggccatg ggcggcgaat tccgcgtcgc
     1621 gacgcgcgcg ggcgaggcgc tcaaggcggt cctgcgtttt ccggcggctt aaaagccgcc
     1681 tctttcaaaa tcgcaacaat ccccgcatag tctcgcccgg atcggctgct aggccgaaaa
     1741 tggaatttcg ggaggctctt tccatgcgtc atcgtctgtt gctcgcctgc gccgcgctgg
     1801 cgctgatcgc cacgcccgcc ctggcccaga tgggcggcgg catgggaggc ggcggcggac
     1861 gcggcggcca tcgcggcggc ggaggcggcg gaggagacgc gtccgacggc aagggcgatc
     1921 agccgacggt cccgacgcgc ccggcgccgc agcaactgcc cgtcgccatg cgtctgggcg
     1981 gcgtcatcga catcatcggc gaaagcaagg ccgccgaggg cggctcggtc gccagcgccc
     2041 ggccggacct gtcggcgatc ttcgtcagcc gcggcggcgc cctgacgctg aaggacgtca
     2101 gcctgagcac cagcggcgcg gtgtcgctgg tcgacgacag ccgcggcagc ggcctcgatt
     2161 cggcgctgct gatcaacgaa agcggccagg cggccatgac gggcggccgc atcagcacca
     2221 gcggcctcgg cgccaacgcc gcctatgtcg ccggtcccgg cgccaggctc aacctgcaag
     2281 gcgtcgtcat cgccaccagc tccagcaacg cctacggcgt cgacgcggtc gacggcgcgg
     2341 cgctgggcgc cgccaacctg acgatcagca cgatgggcga ccacgccccg gctttggcca
     2401 gccgccgcgc cctgccgatg cagctttccg acctgaacct gcacaccgcc gcgtccggct
     2461 cgccgctgct gctggccgag ggcgacatga cggtgaccaa cgccaagggg caggccgagc
     2521 gcgacgacgg gctggccgtg gcgggcgcgc accgcgtcaa cctcacgacc cccgatttgc
     2581 aggccgacgg ctacgcgatc tccgtcgcgg cgggcagcgg cggcgcgcgt ccgggcggcg
     2641 gtccgcaatc cggcgccatg cccggcccca gcctcgcgca ggacgacgcg cccgacgatc
     2701 ccgacgcggg caaggtcaag ccgatcacca gcctgctgag cgctccgccc gacgatcagc
     2761 gcggcgccga cattcaggtc accggcggcg ccctgcgcgg cggccgcgcc gtcctgtcgg
     2821 tcagcaacgc ccacgcgcat atcgtcctga ccggcgtcag cctgagcagc gagtccggcg
     2881 tgatcctgag ggccgccgcc ggtcaggccg gaaccgtggg ccgcaacggc ggcgacgccg
     2941 acctcgaact gcacggcgaa accgtcgcgg gcgacttcgc caccgacgtg atctccagca
     3001 tccgcgtctc gctgctggac cagtcgcacc tgaccggcaa ggccaccgcc aacactgacg
     3061 tcaccctcga cgcgggcagc gactggacgc tgagcgacga cagccatgtc ggcaagctgg
     3121 tcgtgaccga cctcgacaaa cccgacgcca tcgccaacat ccgctccggc ggccacaccc
     3181 tctattacga cccccaccgc aacccctggc tcgaccacaa aacctggccc ctccccggcg
     3241 gcggccaact gacgccggat atctgaagcc gcgaccggac ggcgggcgtt cagaaatacg
     3301 cccgtccgtc cttcaccccc cggtgatagt ccagccgccg gtaaaaggct tcggggtctt
     3361 tcggcttcac cacccatgac ggatcgtggt tgagcaggtc gtagagccgc gtcagggtga
     3421 agcgcaccgc cgcgccgcgc gcgaacaggg gcagggcggc gatctcggcg tccgacaggg
     3481 gccgcgtcgc ctgataggcg cccgcgaagg cggccatcat ctccggctgc gcccgtccgc
     3541 ccggcgtgaa gccccacgca ttgagcgcca ccgccagatc gtaggcgaaa aagtcggtgc
     3601 aggcgtaata atagtcgatc acgccggtga cctgaccgtc ctcgctcatc agcacgttgt
     3661 cggtgaaata gtcggcgtgg atcgcgccgc gcggcaggtc gtcgggccag ttcgcgcgca
     3721 gaaacgccag ctcggcgcga aaatcggcca gaatgtccag cgcgcgcggc gacgtctccg
     3781 ccttcggctc gcagcgttcg atcagccccg gccacgcctt cggccccatc gcgttttccc
     3841 ggaaccgagg gaaatcgccg ccggtcaggt gcagcttcgc cagatattcc cccgccgacg
     3901 ccgcgtggcg cgcatcggga ttgcgcggcc agcgtccggg cagccaccgg atcagcgcgc
     3961 acggcttatc cttgatctca cccaggctcg acccgtcgcg cttcagcgcc ggtcccggcg
     4021 ccggatagcc cttgcggtcg agatagagcg tatagtccat gaaccacggc aggtcgtcca
     4081 ccggcgtcgc cgcctcgaac agggtcagcg catagaggcc c
//
LOCUS       DASVLL010000346         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_183379,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000346 DASVLL010000000
VERSION     DASVLL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183379"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF402_05765"
     CDS             <1..550
                     /locus_tag="VF402_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEX7799835.1"
                     /translation="CERFETRDFETFRLLPMRGAAVFHKALAFFPKRIDGRYAAIGRL
                     DHESLYYLESDDIGVWNNGEPIVQPRYDWELMQVGNCGAPIELDEGWLVITHGVGPVR
                     TYALGAVLLDKADPRKVLARARQPLIAPDAQSRDGYAPNVIYSCGGMKAGDWLFLPYA
                     AADSRICLAAARIRDVLAQLDG"
     gene            827..>1783
                     /locus_tag="VF402_05770"
     CDS             827..>1783
                     /locus_tag="VF402_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HEX7799836.1"
                     /translation="METVVSGQSPAPHRLRRRIYILLSVYMFAYFAAQAMSVSLLSNW
                     LKSTLNLTGEQTGVVFSANFIAALCAQPLYGFISDKVGLRRHVLTFLALMVAGCGFFF
                     AYAYEPLLKTNLILGAGLGGVYLGVTFIAGSFALESFTDRVGRKYGFEYSRARMWGSL
                     GFAFAAFFSGSLFNIDPHINFYIASACALVLLAALAFFRLEATAGERLASSSLKISDA
                     LGVLRLPKFWGFMVLILGVTNLYLVYDQQFPVYYASQFATPALGREMFGRLNSAQIFI
                     EAGMLFLAPFIVNVTGAKRGLLLASAIMIARIAGSFALESFTD"
BASE COUNT          304 a          598 c          523 g          358 t
ORIGIN      
        1 ctgcgaacgc tttgaaacgc gcgatttcga gaccttccgc ctgctgccca tgcgcggcgc
       61 cgccgtcttc cacaaggcgc tcgccttctt cccgaagcgc atcgacgggc gctacgccgc
      121 catcggcagg ctcgaccacg aaagcctcta ttatctcgaa agcgacgata tcggcgtctg
      181 gaacaacggc gagccgatcg tgcagccgag gtacgactgg gagctgatgc aggtcggcaa
      241 ttgcggcgcg cccatcgagc tggacgaagg ctggctggtg atcacgcacg gcgtcgggcc
      301 ggtgcggacc tacgccctgg gcgcggtcct gctcgacaag gccgacccgc gcaaggtgct
      361 ggcgcgcgcg cgccagccgc tgatcgcgcc cgacgcgcaa tcgcgggacg gctacgcgcc
      421 gaacgtcatc tacagttgcg gcggcatgaa ggcgggcgac tggctgttcc tgccctacgc
      481 cgccgccgac agccgcatct gcctcgctgc ggcccgcatc cgcgacgtgc tggcgcaact
      541 ggacggctag agcgctttcc gaaaagtgtg acgcactttt cggattaaaa agcgcgtaaa
      601 aatataaagt tagagcgccg ccgcttctct ctttctgtcc gatcctcccc gtccggcgtt
      661 caaaagccgg acgccgtccc gtctttgcat gagatgcgcg ccgggccgct caaaaagaag
      721 tgtacaaacg ccatgatggg tgatatcgat atcagaaatt ccggcaagac gccacgcctg
      781 tcgcgccccg ccggcccaaa aacaacgcca ggaaacacaa tacgggatgg aaacggtggt
      841 tagcggccag agccccgcac cgcacaggtt gcggcgcaga atctacatct tgctgagcgt
      901 ctatatgttc gcctatttcg cggcgcaggc gatgagcgtc tcccttttgt cgaactggct
      961 gaaaagcacg ctgaatctga caggcgaaca gacgggcgtc gtcttttccg ccaacttcat
     1021 cgccgccctg tgcgcccagc cgctctacgg tttcatttcc gacaaggtcg gtctgcgccg
     1081 tcacgtcctg acctttctgg cgctgatggt ggcgggctgc ggattcttct tcgcctacgc
     1141 ctacgagccg cttttgaaga ccaatctcat tctgggcgca ggccttggcg gcgtctatct
     1201 gggcgtgacc ttcatcgctg gctctttcgc gctggaatcg ttcaccgacc gggtcgggcg
     1261 caaatacggc ttcgaataca gccgcgcccg gatgtggggc tcgctcggct tcgccttcgc
     1321 cgccttcttt tcgggcagcc tgttcaatat cgatccgcac atcaacttct acatcgcctc
     1381 ggcctgcgcg ctggtcctgc tggccgcgct cgcctttttc cggctcgaag cgacggcggg
     1441 cgagcgcctc gcctcgtcgt cgctgaaaat atccgacgct ctgggcgttc tgcgcctgcc
     1501 gaaattctgg ggcttcatgg tcctgattct cggcgtgacc aacctgtacc tcgtctacga
     1561 ccagcagttc ccggtctatt acgcctcgca gttcgccacg cccgcgctcg gacgggaaat
     1621 gttcggacgg ctgaactccg cccagatctt catcgaagcg ggcatgttgt ttctggcgcc
     1681 cttcatcgtc aacgtcacgg gcgcgaaacg cggcctgctg ctggcctcgg ccatcatgat
     1741 cgcgcgcatc gccggctcct tcgcgctgga atcgttcacc gac
//
LOCUS       DASVLL010000347         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_75796, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000347 DASVLL010000000
VERSION     DASVLL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75796"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VF402_05775"
     CDS             <1..1338
                     /locus_tag="VF402_05775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7799837.1"
                     /translation="YVSARLAGTTEPWSTNFDIYSVPVTGGAPTDLTEANKAWDAAPR
                     VSPDGRTLAYTAMKRPGFESDRFDLMLRDLATGKVTPLAPDWDHTVAGLQWARDGRSL
                     LVDADDHGRHLLFRVDVKTGKVAQLSKDGDMAAYEETPHGLVFLKNSLKRPNTLYLAA
                     KNAAFVDDGAKNLTRFDSVLDTLAEGDYEQFRFKGWKDEDVYGYVMKPANYQAGKTYP
                     VAFIVHGGPQVAFGDGWSFRWNPETYAGAGYAVVFIDFHGSPGYGQAFTDSISQHWGD
                     RPLEDLQKGWAYALAHYTFLDGDRACALGASYGGFMINWIAGNWPQPWKCLVSHDGVF
                     DARGMGYNTDEMWFEHWEQGADPYDDPAAYDTFNPALHVKDWKDPMLVIHSDQDFRIP
                     MAQGIGAFTALQAKGIESEFLRFPDESHWVSKPQNSLMWHRTVFDWLDKHLKP"
     gene            complement(1433..>1674)
                     /locus_tag="VF402_05780"
     CDS             complement(1433..>1674)
                     /locus_tag="VF402_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="selenoprotein O"
                     /protein_id="HEX7799838.1"
                     /translation="LGDSPRAGHYRGPEFDAFKAALDAYEPMHRKHPRLLQPDPETLL
                     YDEIEAIWARIDEADDWSLFDAKLAAIDVYREALA"
BASE COUNT          319 a          498 c          560 g          297 t
ORIGIN      
        1 tatgtctcgg cgcggctggc cgggaccacc gagccgtgga gcaccaattt cgacatctac
       61 agcgttcccg tcaccggcgg cgcgccgacc gacctgaccg aagccaacaa ggcgtgggac
      121 gcggcgccgc gcgtctcgcc cgacggcagg acgctggcct ataccgccat gaagcggccg
      181 ggcttcgagt ccgaccgctt cgacctgatg ctgcgcgatc tggccacggg caaggtcacg
      241 ccgctggcgc cggactggga tcacacggtc gcggggctgc aatgggcgcg ggacggcagg
      301 tcgctgctgg tcgacgccga cgatcatggc cggcatctgt tgttccgcgt cgatgtcaag
      361 accggcaagg tcgcgcagtt gtcgaaagac ggcgacatgg cggcctatga ggagacgccg
      421 cacgggctcg tctttctcaa gaacagcctg aaacgtccga acacgcttta tctggcggcg
      481 aaaaacgcgg ccttcgtcga tgacggcgcg aagaacctga cgcgcttcga cagcgtgctc
      541 gataccttgg ccgaaggcga ttacgagcaa ttcagattca agggctggaa ggacgaggac
      601 gtgtacggct atgtgatgaa gcccgccaat tatcaggcgg gcaagaccta tccggtcgcc
      661 ttcatcgtcc acggcgggcc gcaggtcgcg ttcggcgacg gctggtcgtt ccgctggaac
      721 ccggaaacct atgcgggcgc gggctatgcg gtggtgttca tcgactttca cggctcgccc
      781 ggctacgggc aggccttcac cgattcgatc tcgcagcact ggggcgaccg gccgctggag
      841 gatctgcaaa agggctgggc ctatgcgctg gcccattaca cgtttctcga cggcgaccgg
      901 gcctgcgcgc tgggggcgtc gtatggcggc ttcatgatca actggatcgc cgggaactgg
      961 ccgcagccgt ggaagtgcct ggtcagccac gacggcgtgt tcgacgcgcg cggcatgggc
     1021 tacaacaccg acgaaatgtg gttcgagcat tgggagcagg gcgccgatcc gtatgacgat
     1081 ccggcggcct acgacacatt caatccggcc ctgcatgtga aggactggaa agacccgatg
     1141 ctggtcatcc attccgatca ggatttccgc atcccgatgg cgcagggcat cggcgccttt
     1201 accgccttgc aggcgaaggg aatagaatcg gaattcctgc gttttcccga tgaaagccac
     1261 tgggtgagca agccgcagaa ttcgctgatg tggcacagga cggtgttcga ctggctggac
     1321 aagcacctga aaccttagag cgctttccga aaagtgtgac gcacttttcg gattaaaaag
     1381 cgcgtaaaag cataaaacta gagcgccgat ctgatgcaat cagatcggcg ctctaagcga
     1441 gcgcctcgcg atagacgtcg atggcggcca gcttggcgtc gaacagggac cagtcgtcgg
     1501 cctcgtcgat gcgcgcccag atggcttcga tctcgtcgta gagcagggtt tcgggatcgg
     1561 gttgcagcag gcgcggatgc ttgcgatgca tcggttcata ggcgtcgagc gcggccttga
     1621 aggcgtcgaa ctccggcccg cgatagtgtc cggcgcgcgg gctgtcgccg aggc
//
LOCUS       DASVLL010000348         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_343696,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000348 DASVLL010000000
VERSION     DASVLL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343696"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..442
                     /gene="rbfA"
                     /locus_tag="VF402_05785"
     CDS             41..442
                     /gene="rbfA"
                     /locus_tag="VF402_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7799839.1"
                     /translation="MRAGELVRHALVEILREEDIHDDDLHGVSITVTEVRCSPDLRHA
                     TVFVEPLGAGLGGVTITADEVGPAVAALNRHAKFLRGILGRMIDMKFTPDLRFVHDES
                     FNEAARIDALFMRPDIARDLHHDDDADEDQD"
     gene            579..1529
                     /gene="truB"
                     /locus_tag="VF402_05790"
     CDS             579..1529
                     /gene="truB"
                     /locus_tag="VF402_05790"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7799840.1"
                     /translation="MARKKKGDPIHGWICFDKPLEMTSTAAVGKIRWLFQAQKAGHAG
                     TLDPLATGVLPIALGEATKTVPYLMEADKVYSFTIAFGTTTDSFDAEGRVTAQSDARP
                     TEGAISKALPAFIGVIEQVPPAFSAIKVDGRRAYDLAREGVEVELKAREVEIFDLALD
                     AFHGDSADFTLHCGKGTYVRSVARDLCAELGVCGHVSKLRRESVGAFETKDAITLENL
                     EELVHRGAHLEALLAVGTALDDIPALPVTPDEAARLKQGRDLVLLPRQIDLIFEALKA
                     RREAGFAEFPDTVQAVCGGQVIALCEARGRHLHPVRILNL"
     gene            1557..1826
                     /gene="rpsO"
                     /locus_tag="VF402_05795"
     CDS             1557..1826
                     /gene="rpsO"
                     /locus_tag="VF402_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7799841.1"
                     /translation="MSITADRKSEVIKSHARAANDTGSAEVQIAILSERIANLTEHFK
                     EHKKDNHSRRGLLKMVSQRRRLLDYLKNRHADRYQAIIETLGLRR"
     gene            2089..>2961
                     /locus_tag="VF402_05800"
     CDS             2089..>2961
                     /locus_tag="VF402_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7799842.1"
                     /translation="MFDIKRKSIEWAGRKLTLETGRLARQADGAVLATYGETTVLATA
                     VFARAPKPGQDFFPLTVNYQEKTFAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPL
                     FVKGFKNEVQVVCTVLSHDMENDPDIVAMVAASAALTLSGVPFMGPIGGARVGVIDGE
                     YVLNPTLDQMKDSDLDLVVAGTEDALMMVESEARELTEDKMLGALMFAHKGMQPVIEA
                     IIELAEHAAKEPFAFEAEDMSGVVAEIKKLVGDDIRNAYSIKEKTARHDAVGAAKAKA
                     GAALLKSEENPEGVE"
BASE COUNT          559 a          933 c          967 g          502 t
ORIGIN      
        1 tggtcgcgct cccgccgagc aggggccgag ccagcgccag ttgcgcgccg gcgaactggt
       61 gcggcatgcg ctggtcgaga ttctgcgcga ggaagacatc cacgacgacg acctgcacgg
      121 cgtctcgatc accgtgaccg aggtgcgctg ctcgcccgat ctgcgccacg ccaccgtgtt
      181 cgtcgagccg ctgggcgcag ggctgggcgg cgtgaccatc accgccgacg aggtcggtcc
      241 cgccgtcgcg gcgctcaacc gccatgcgaa gtttttgcgc gggattctgg gccggatgat
      301 cgacatgaag ttcacgcccg acctgcgttt cgtgcatgac gaaagcttca acgaggcggc
      361 gcgcatcgat gcgctgttca tgcggcccga catcgcgcgc gatctgcatc atgacgatga
      421 tgcggacgag gatcaggatt aacccgtctc ccctgcttta gcgggggagg cgtcccggct
      481 tgtccgtgac ggagggggcg ccccgccgtc cgcaggcccc ctccggcgag acaagctcgc
      541 cacctccccc gctaaagcag ggggaggagg aataagacat ggcccgaaag aaaaaaggcg
      601 atccgatcca cggctggatc tgtttcgaca agccgctgga gatgacctcg accgcggcgg
      661 tcggcaagat acgctggctg tttcaggcgc agaaggccgg gcatgccggc acgctggacc
      721 cgctggcgac cggcgtcctg ccgatcgcgc tgggcgaggc gaccaagacc gtgccgtacc
      781 tgatggaggc ggacaaggtc tatagtttca ccatcgcgtt cgggaccacg accgactcgt
      841 tcgacgccga aggccgcgtg acggcgcagt cggacgcgcg tccgacggaa ggcgcgataa
      901 gtaaggccct gcccgccttt atcggcgtga tcgagcaggt tccgcccgct ttttccgcca
      961 tcaaggtcga cggcaggcgc gcctacgatc tggcgcgcga gggcgtggag gtcgagttga
     1021 aggcgcgcga ggtcgagatt ttcgatctgg cgctcgacgc ctttcacggc gacagcgccg
     1081 acttcaccct gcattgcggc aagggcacct atgtgcgctc cgtcgcccgc gacctgtgcg
     1141 cggagcttgg ggtttgcggc catgtatcga agctcaggcg cgagagcgtc ggcgcgtttg
     1201 aaacaaaaga tgcgattaca ctggaaaatc tcgaggaatt ggtgcataga ggcgcgcatc
     1261 ttgaggcttt gctcgccgtc gggacagcgc tggacgacat cccggccctg cccgtgacgc
     1321 ctgacgaggc cgcaagattg aagcaaggcc gtgaccttgt cctcctgccc cgtcagatag
     1381 accttatctt cgaggctctc aaagcccgcc gcgaggccgg ttttgccgag tttcccgata
     1441 cggttcaggc ggtgtgcggc ggccaggtga tcgcgctttg cgaagcgcgc gggcggcatc
     1501 tgcatcccgt gcggattctg aacctttgat ccttgtttta gaaaaggaaa cacccgatgt
     1561 cgattactgc tgaccgcaag tcggaagtca tcaagagcca cgcccgcgcc gccaacgaca
     1621 ccggcagcgc cgaagtgcag atcgcgatcc tgtcggaacg catcgccaac ctgaccgagc
     1681 acttcaagga acacaagaag gacaatcaca gccgccgcgg cctcctgaaa atggtgtcgc
     1741 agcgtcgccg ccttctcgac tacctgaaga accgccatgc ggaccgctat caggcgatta
     1801 tcgaaaccct cggcctgcgc cgctaggttt caaagtttca aaagggtctg ggggcgactt
     1861 cagacccttt tgtccatccc ggagttcgcc tgtccggcga gcttcgcgct ccggccgcct
     1921 gctttcaagg cggctgatct catatccggc ggggtgatcc gcatgtcgcc ggaaatccac
     1981 agggttcaag gcgttagata acttctcacg aacccttcat tacgccgctc cgatagcggc
     2041 gggccttggc ggcatggggc tgtccggggc cagaacgaaa gacgacatat gttcgatatc
     2101 aaaagaaaat ccatcgagtg ggccggccgc aagctgacgc tcgaaaccgg ccgtctggcc
     2161 cgtcaggccg acggcgccgt gctggccacc tatggcgaga cgaccgtgct ggccaccgcc
     2221 gtgttcgccc gcgcgcccaa gccgggtcag gactttttcc cgctgacggt caactatcag
     2281 gaaaagacct tcgcggccgg caagattccc ggcggctatt tcaagcgcga aggccgtcct
     2341 tcggaaaagg aaacgctggt ttcgcgcctg atcgaccgtc cgatccgccc gctgttcgtc
     2401 aagggcttca agaacgaagt gcaggtcgtc tgcaccgtcc tgtcgcacga catggagaat
     2461 gatcccgaca tcgtggcgat ggtcgcggcc tcggcggcgc tgaccctgtc gggcgtgccg
     2521 ttcatgggcc cgatcggcgg cgcccgcgtc ggcgtgatcg acggtgagta cgtgctgaac
     2581 ccgacgctgg atcagatgaa ggactcggac ctcgacctgg tggtcgcggg caccgaggac
     2641 gcgctgatga tggtcgagtc cgaagccagg gagctgaccg aggacaagat gctgggcgcg
     2701 ctgatgttcg cgcacaaggg catgcagccg gtgatcgagg cgatcatcga gctggccgaa
     2761 cacgcggcca aggagccgtt cgcgttcgag gccgaggaca tgtccggcgt cgtggccgaa
     2821 atcaagaagc tggtcggcga cgacatccgc aacgcctatt ccatcaagga aaagaccgcg
     2881 cgtcacgacg ccgtgggcgc cgccaaggcc aaggccggcg cggccctgct gaagtcggag
     2941 gaaaaccccg aaggcgtcga g
//
LOCUS       DASVLL010000349         6014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_21961, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000349 DASVLL010000000
VERSION     DASVLL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21961"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..844
                     /locus_tag="VF402_05805"
     CDS             2..844
                     /locus_tag="VF402_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX7799843.1"
                     /translation="MSAIACAVMTLCAATPLYAKTVSHAHARISHVKASHRKASGSTP
                     YLQCVTFARQLTGMQIFGDAWTWWEKATGKYDEGHAPKPGAVLVFRPQGKMRLGHVAV
                     VSQIITDRYIQVTHANWSPINGRRGQVEDHVNVLDVSEKGDWSKVKVWYGPLNDLGTT
                     VYDTYGFIYNDAPQTVAAPETAAPSSPMTAAATQQVAMNDASAIAPADLPADLKSALT
                     PGNTQGFGAVKPVTVAARIKGKAAADIVARLDDDKPVVKPEAKPAAHKTVVKTHARRK
                     THRR"
     gene            876..1832
                     /locus_tag="VF402_05810"
     CDS             876..1832
                     /locus_tag="VF402_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX7799844.1"
                     /translation="MLSLHAAGQTSDPRARDSDAAWIDMFQPTRDEELAMEARLGVLL
                     PTREDMAEIETSSRLYLENGAAFMTAQVAFFGGQEHLQSGPVTFVLAAQKLVTIRYID
                     PASFAIFAGHIDKQPVLCLDGPTAFLNLLDVIIDRTADLIERTQGGIDELSRSIFTRN
                     RKGRLEDVLILLGNAQNDIVKIRDSLVGFARLTAFAAGLDPALTRGTHTQTKEFHDRL
                     HTLSQDVASLSDHATYVSGNIAFLLDAALGLINVEQNSVMKLISVTSVVFLPLTLIAS
                     IYGMNFDHMPFLHDPDAFWASMAIMAVIAVALVWWFRMKRWV"
     gene            complement(1837..2313)
                     /locus_tag="VF402_05815"
     CDS             complement(1837..2313)
                     /locus_tag="VF402_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX7799845.1"
                     /translation="MSTTCDHDHAHAHALPPREKTALLEEAQALCAEAGERMTTSRLR
                     TYELILEAPGPIKAYDVIDRFHPDGAAKPPTVYRALSFLEQMGLIHRIESLNAFVACD
                     TRDHPHTAAFLLCDCCGSSEEIAIPAVPEIEAAAARTGFQVKHVTLEAHGLCRGCQ"
     gene            2471..4612
                     /locus_tag="VF402_05820"
     CDS             2471..4612
                     /locus_tag="VF402_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799846.1"
                     /translation="MTSHIRSLIAFGASALALSAALNVGLTVPAAHAQDAAKKARDDR
                     PVEVVVRAHRLDAARDSIQPDIGASSFALSKAAIQTLPGGDNLPLNQVVLQAPGVVQD
                     SYGQLHVRGDHNGVQYRLNGVILPEGLSNFGNVLSPRFAAGVQLITGALPAQYGLRTA
                     GVINITTQSGVKNGGEMSIYGGSHGDYEPSVSYGGSRGNDTFFGSLSYKQNQLGIEAP
                     YNASTPLHDRTSQIMGFGYYDHMIDDDSRVSLIAGVSDQAFQIPNTPGLNSMDDGAGY
                     AVNGVDSFASAALNGRQRETTDYVIASYLKTTGDFTGQVSVFARASRLTYQPDWDGEL
                     AFNGVSQYADKRDTSAGIQAEGAWTLSARHTLRAGVIVSGDRSVSRTVSHVFRLDADG
                     DQTSDTPVVIADNGARSNLTESLYVQDEWIPFRDFTLNYGLRYDKLDSYRTEDQLSPR
                     VNFVWTPLKGMTVHGGYARYFTPPPYELIASQTQALFAGTSFDISGQNDLPYAERDNY
                     YDLGVQQRIDAHLTVGVDAYSRVARYLIDEGQFGAPIILTPFNYKYGRNKGVELTANY
                     QNGPFTLYGNYAAGSARGRQIVSSQFNFDTDDQAYVADHFIYVDHNQTTTASAGAAYR
                     FGKTRLSLDALYGSGLRTDGDIPNGLHLPSYTQVNASLAHDFAHGISARLDVINLFDA
                     AYEIRDGGGIGVGAPQWGPRRGVYAGLSKSF"
     gene            4712..5500
                     /locus_tag="VF402_05825"
     CDS             4712..5500
                     /locus_tag="VF402_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX7799847.1"
                     /translation="MAQEPTNDATLSGNVLFYKNPEPLNPEAHGGMGIKAVPNPHAFV
                     AETNVVPLTVSEFPAASLSYPIVFIGENYMPVAAMGLSSGQNVFVSPEGAFRPDSYLP
                     AFARRYPFVFANDEQQQRLILCVDLEAPMVVKSSPDVAFFENGQATAYVENAMQFCSD
                     FENERRRTESFVQLLRDLDLLAPRETVYRPQNPDGTAGEPQKIAEYFAVDDKKLNALS
                     GEKLVELRDNGALTHIYAHLNSLLAWDRLIALTIERNNAQGQAN"
     gene            complement(5581..5970)
                     /locus_tag="VF402_05830"
                     /pseudo
     CDS             complement(5581..5970)
                     /locus_tag="VF402_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
BASE COUNT         1094 a         2100 c         1830 g          990 t
ORIGIN      
        1 cgtgtcggcg atagcctgtg ccgtaatgac cctgtgcgcc gccacgccgc tttacgcgaa
       61 aaccgtcagc cacgcccacg cccggatcag ccacgtcaag gcttcgcacc gcaaggcgtc
      121 cggctccacc ccctatcttc aatgcgtcac cttcgcccgt cagttgaccg gcatgcagat
      181 tttcggcgac gcctggacct ggtgggaaaa agccaccggc aagtatgacg aaggccacgc
      241 gccgaaaccg ggcgccgtct tggtgttcag gccgcagggc aagatgcgcc tcggccacgt
      301 cgccgtcgtc tcgcagatca tcaccgaccg ctatattcag gtgacccacg ccaactggtc
      361 gccgatcaac ggccgccgcg gccaggtcga ggaccacgtc aacgtgctcg acgtctccga
      421 aaagggcgac tggagcaagg tcaaggtctg gtacggcccg ctcaacgacc tcggcacgac
      481 ggtctatgac acctacggct tcatctataa cgacgcgccg cagaccgtgg ccgcgccgga
      541 aaccgccgcg ccgtcctcgc cgatgaccgc cgccgcgacg cagcaggtgg cgatgaacga
      601 cgccagcgcc atcgcgcccg ccgacctgcc cgccgatctc aaatccgccc tgacgcccgg
      661 caacacgcag ggcttcggcg ccgtcaagcc ggtgaccgtc gccgcccgca tcaagggcaa
      721 ggccgccgcc gacatcgtgg ccaggctgga cgacgacaag ccggtcgtga agccggaagc
      781 caaacccgcc gcgcacaaga cggtcgtcaa gacccacgcg cgcaggaaga cgcaccggcg
      841 ctaaacccca cggctaaaaa agaaagcggg gggacatgct gagtctgcac gccgccggcc
      901 agacttccga tccgcgggcg cgcgattcgg acgcggcctg gatcgacatg tttcaaccga
      961 cccgcgacga ggaactggcg atggaggcgc gcctcggcgt gctgttgccg acgcgcgagg
     1021 acatggccga gatcgagacg tccagccgtc tctatctgga gaacggcgcc gccttcatga
     1081 ccgcgcaggt cgccttcttc ggcggtcagg agcatctgca aagcgggccg gtgaccttcg
     1141 tgctggcggc gcagaagctg gtgaccatcc gctatatcga cccggcctcg ttcgcgatct
     1201 tcgccgggca tatcgacaag cagcccgtgc tgtgcctcga cggcccgacc gcgtttctca
     1261 atctgctcga cgtcatcatc gaccgcaccg ccgacctgat cgagcgcacg cagggcggca
     1321 tcgacgagct gtcgcgctcg atcttcacgc gcaaccgcaa ggggcggctg gaggacgtgc
     1381 tgatcctgct cggcaacgcg cagaacgaca tcgtgaagat ccgcgattcg ctggtcggct
     1441 tcgcgcggct gaccgccttc gcggcggggc tggacccggc cctgacgcgc ggaacgcaca
     1501 cccagacgaa ggagtttcac gaccggctgc acaccctcag tcaggacgtg gcctcgctca
     1561 gcgaccacgc cacctatgtg tcgggcaata tcgccttcct gctcgacgcg gcgctgggcc
     1621 tgatcaatgt cgagcagaat tcggtgatga agctgatctc ggtgacctcg gtggtgtttc
     1681 tgcctctgac cctgatcgcg tcgatctacg gcatgaattt cgaccacatg ccgttcctgc
     1741 acgaccccga cgccttctgg gcgtcgatgg cgatcatggc cgtcatcgcg gtcgcgctgg
     1801 tctggtggtt ccgcatgaag cggtgggtgt gacggcctac tgacagccgc ggcagaggcc
     1861 gtgcgcctcc agcgtcacat gcttgacctg aaagccggtg cgcgccgccg ccgcttcgat
     1921 ttccggcacg gcgggaatgg cgatctcctc gctggagccg cagcagtcgc agagcaggaa
     1981 ggccgccgtg tgcggatggt cccgcgtgtc gcaggcgacg aaggcgttaa ggctctcgat
     2041 gcggtggatc agccccatct gctccagaaa gctgagcgcg cgatagacgg tcggcggctt
     2101 ggccgcgccg tcgggatgga agcggtcgat cacgtcatac gccttgatcg gccccggcgc
     2161 ctccaggatc agctcatagg tgcgcaggcg cgacgtcgtc atgcgctcgc ccgcctcggc
     2221 gcacagggcc tgcgcctcct ccagaagggc ggtcttttcg cgcggcggca gcgcatgggc
     2281 gtgggcgtga tcatgatcgc aggtcgtact catatcccta aggtaggatg agttgcgctt
     2341 tttcgcaacg gttgcggcgg tttgcggcag gccgtgataa aaagtgtaat actataacaa
     2401 ttgcgcttga cctaagttac aatattacat aacaaatgcg gcgatgcaac attatcgccg
     2461 gagccgccgc atgacctcgc atatccgttc cctcatcgcc ttcggcgcgt cggcgctggc
     2521 gctgagcgcc gccctgaacg ttggcctgac cgttccggcg gcgcatgcgc aggacgcggc
     2581 gaaaaaggcg agggacgacc ggcccgtcga ggtcgtggtc cgcgcgcacc gcctcgacgc
     2641 cgcgcgcgat tccatccagc cggacatcgg cgcctccagc ttcgcgctgt cgaaggcggc
     2701 gatccagacc ctgcccggcg gcgacaacct gccgctcaat caggtggtgt tgcaggcgcc
     2761 cggcgtggtg caggattcct atggccagtt gcatgtgcgc ggcgaccata acggcgtgca
     2821 ataccggctg aacggcgtga tcctgcccga aggcctgagc aatttcggca atgtgctgag
     2881 cccgcgcttc gccgccgggg tgcagttgat caccggcgcc ctgcccgcgc aatacggcct
     2941 gcgcacggcg ggcgtgatca acatcaccac ccagtcgggc gtcaaaaacg gcggcgagat
     3001 gtcgatctac ggcggcagcc acggcgacta cgagccgagc gtctcctacg gcggctcgcg
     3061 cgggaacgac accttcttcg gctcgctgag ctacaagcag aaccagctcg gcatcgaggc
     3121 gccgtacaac gcctcgaccc cgctgcacga ccgcacctcg cagatcatgg gcttcggcta
     3181 ttacgaccac atgatcgacg acgacagccg cgtctcgctg atcgcgggcg tgtcggatca
     3241 ggcgttccag atccccaaca cgccgggcct caactcgatg gacgacggcg cgggctacgc
     3301 cgtcaacggc gtcgacagct tcgccagcgc ggcgctgaac ggccgccagc gcgaaaccac
     3361 cgactacgtc atcgcctcgt acctgaagac caccggcgat ttcacgggcc aggtttccgt
     3421 attcgcgcgc gcctcgcgcc tgacctatca gccggactgg gacggcgaac tggcgttcaa
     3481 cggcgtcagc cagtacgccg acaagcgcga cacgtccgcc gggattcagg ccgagggcgc
     3541 ctggacgttg agcgcgcgcc ataccttgcg cgcaggcgtg atcgtgtccg gcgaccgcag
     3601 cgtgtcgcgc accgtgagcc acgtcttccg tctcgacgcg gacggcgatc agacctcgga
     3661 cacaccggtc gtcatcgccg acaacggcgc ccgctcgaac ctgacggaaa gcctctatgt
     3721 gcaggacgaa tggattccgt tccgcgactt cacactcaat tacggcctgc gctacgacaa
     3781 actcgattcc taccgcaccg aggatcagtt gagcccgcgc gtcaacttcg tctggacgcc
     3841 gctgaagggc atgaccgtgc atggcggcta tgcgcgctac ttcacgccgc cgccctatga
     3901 gctgatcgcc tcgcagactc aggcgctgtt cgccggaacc tcgttcgaca tttccgggca
     3961 aaacgacctg ccctacgccg agcgcgacaa ctattacgac ctcggcgtcc agcagcggat
     4021 cgacgcgcat ctgaccgtcg gcgtcgacgc ttacagccgc gtcgcgcgct acctgatcga
     4081 cgagggccag ttcggcgcgc cgatcatcct gacgccgttc aattacaaat acggccgcaa
     4141 caagggcgtc gaactcaccg ccaactacca gaacggcccg ttcacccttt acggcaatta
     4201 cgcggccggt tcggcgcggg gccgccagat cgtgtcgtcg cagttcaatt tcgacacgga
     4261 cgatcaggcc tatgtcgccg accacttcat ctatgtcgac cacaaccaga cgaccaccgc
     4321 ctcggcgggc gcggcctatc gcttcggcaa gacgcgcctg tcgctggacg cgctgtacgg
     4381 ctccggccta cgcaccgacg gcgacattcc caatggcctg catctgccgt cctacacgca
     4441 ggtcaacgcc tcgctcgcgc acgacttcgc ccacgggatc agcgcccgcc tcgatgtgat
     4501 caacctgttc gacgcggcct acgaaatccg cgacggcggc ggcatcggcg tcggcgcgcc
     4561 gcaatggggg ccgcgccgcg gcgtctatgc cggtctgagc aagagcttct gaggcgcgga
     4621 tttcccccga caggtcacaa agcttgccgc ccttcgagcc atgcgttaga agcatggctc
     4681 gtttgtttca actcaggcaa ttataaactt catggcccaa gaacccacca acgacgccac
     4741 gctgtccgga aacgtcctct tctacaagaa tcctgagccg ctgaatcccg aagcgcatgg
     4801 cggcatgggc atcaaggccg tgccgaatcc gcacgccttc gtggccgaaa ccaatgtcgt
     4861 gccgctgacc gtctcggaat ttccggcggc ttcgctcagc tatcccatcg tctttatcgg
     4921 cgagaactac atgccggtcg cggccatggg cctgtcgtcc gggcaaaacg tgttcgtctc
     4981 gcccgaaggc gccttccggc ccgattccta cctgccggcc ttcgcgcgcc gctatccgtt
     5041 cgtcttcgcc aacgacgagc agcagcagcg cctgatcctg tgcgtcgacc tcgaggcgcc
     5101 gatggtggtc aagagcagcc cggacgtcgc cttcttcgag aacggccagg ccacggccta
     5161 tgtcgaaaac gccatgcagt tctgctcgga cttcgagaac gagcgccgcc gcaccgaatc
     5221 gttcgtgcag ttgctgcgcg acctcgacct gctggcgccg cgcgaaaccg tctatcgtcc
     5281 gcaaaacccg gacggcacgg cgggcgagcc gcagaagatc gccgaatatt tcgccgtcga
     5341 cgacaagaag ctgaacgcgc tgtcgggtga aaaactggtc gagctgcgcg acaacggcgc
     5401 cctgacgcac atctacgcgc acctgaactc gctgctggcc tgggaccgtc tgatcgccct
     5461 gaccatcgag cgcaacaacg ctcagggtca ggcgaactga gacgcaacgc cattaaaaaa
     5521 gaaaggctcc ggttttccgg agcctttttt tatgactctg gcttcgggcg ccttcgggtt
     5581 gcgctggaac accgaacgcg tcaggcagga aaagaccagc cgcccggcct cgtccagcag
     5641 gtcgttctgc gcgatgacca cgcccttggt gtcgccgacc gcgtccttgc ccatcacggt
     5701 cagccgcccg cgcagcagtt cgcccgccgg cgggttgcgc gaccagcgca gcgcgtcgac
     5761 cgccagccgc ttggtctgcg gccagttcag cgccgcctcg ctctccagcc gcgaccacag
     5821 ggcgaagatc agcgcgtcgg gcaggccgtc gcctgcgtcc cagtgcggcg cataggtctt
     5881 gcaaaacgcc gccagctcgc tgtccgacac caccccggcg cccagcgacg ccacctgtcc
     5941 gatccacaaa tcgtcgaaag ccgcaggcat gacggactcc ctgtatccgc ttacacgagg
     6001 cgtccgtctt accc
//
LOCUS       DASVLL010000350         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_452983,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000350 DASVLL010000000
VERSION     DASVLL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452983"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1222)
                     /locus_tag="VF402_05835"
     CDS             complement(80..1222)
                     /locus_tag="VF402_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7799848.1"
                     /translation="MPEDDIQPFLTLDVSRAAYRMADEGRPVIHMEFGQPSTGAPKAA
                     IAAAHRVLDSDPMGYWESVPLKARLSRHYEETYGLDIAAERFILTCGASPALVLALTA
                     AFRPGARIALARPGYVAYRNTIRALGMVPVEIPCGAEARFQLSAAALSALDPAPDGVI
                     VASPANPTGTILPEAELRAIAGVCRARRIRIVSDEIYHGLSYIGPTPSILQCAPDALV
                     INGFSKYFSMVGWRLGWLVAPENLRHKARALMSNLFLTPPSLSQHAALVAMDAREDLQ
                     TYAETYARNRQLLIDALPALGLNRIAPPDGAFYIYADIGHLTDDSPILDSLAFCHRML
                     AETGVAAATGRDFDPVDGGRFIRFSFAVSTEETREAIRRLTDWFAR"
     gene            complement(1231..>2050)
                     /locus_tag="VF402_05840"
     CDS             complement(1231..>2050)
                     /locus_tag="VF402_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7799849.1"
                     /translation="KIGAGHIDPILLTSLRWGIAAAVAVALSLKQLRADWPVIRRHGV
                     MLFAYGASGFAIFNILLYTALTKTSVINVMIEQAAIPFVIFTGNFALLRQRTTTAQLI
                     GGALTLGGVVITATHGHPEGLLRLKLNAGDALMLLAVVVYAGYSVGLKGKPPLHWRSF
                     LAAPCLAAALTCVPFLIWRAASAPLAAPDLTGWLVALYAAVFVALISSATWVAGVELI
                     GANRAALFINLLPIFGILQSIVLLHARLEGFHLIAIALVGGGIVLAETQRLRQH"
BASE COUNT          384 a          637 c          716 g          313 t
ORIGIN      
        1 ccgtcaggcg acaatcagac aatcagacga taaggcgcta tgcggatttc gtgtcctttc
       61 gtgtttttcg tggcaaaaac taccgcgcga accagtccgt caatcgccgg atcgcctcgc
      121 gcgtttcctc ggtcgagacg gcgaagctga agcggatgaa gcgccctccg tcgacgggat
      181 cgaagtcgcg tccggtcgcc gccgccacgc cggtttccgc cagcatcctg tgacagaagg
      241 ccaaactatc aaggatcggg ctgtcgtccg tcagatggcc gatgtcggca tagatgtaaa
      301 aagcgccgtc cggcggcgcg atccggttca ggcccagcgc gggcagggcg tcgatcaaca
      361 gttgacgatt gcgcgcgtaa gtttcggcgt aagtctgcaa atcctcgcgc gcgtccatgg
      421 cgacgagggc ggcgtgctgg ctcagcgacg gcggcgtcag gaacaggttg ctcatcaggg
      481 cgcgcgcctt gtggcgcaga ttttcgggcg cgaccagcca gccgagccgc cagccgacca
      541 tgctgaaata tttggagaaa ccattgatca ccaaagcgtc cggcgcgcat tgcaggatcg
      601 acggcgtcgg gccgatgtaa gaaaggccgt gatagatttc gtcggacacg atgcggatgc
      661 ggcgcgcgcg gcagaccccg gcgatggcgc gcaactcggc ttcgggcagg atcgtgccgg
      721 tcggattggc cgggctggcg acgatcacgc cgtccggcgc cgggtcgagc gcgctcaggg
      781 cggcggcgct gagctggaag cgcgcctccg cgccgcacgg aatctcgacc gggaccatgc
      841 ccagcgcgcg gatcgtgttg cgataggcga catagccggg ccgcgccagc gcgatgcgcg
      901 cgccgggcct gaacgccgcc gtcagggcca gcaccagcgc gggcgaggcg ccgcaggtca
      961 ggatgaaacg ctcggcggcg atgtcgaggc cgtaggtttc ctcataatgc cgcgacaggc
     1021 gcgctttgag cgggacgctt tcccaatagc ccatcgggtc ggaatcgagc acgcggtgcg
     1081 cggcggcgat ggcggccttg ggcgcgccgg tcgagggctg gccgaactcc atatggatga
     1141 ccgggcggcc ttcgtccgcc atccggtagg cggcgcggct gacgtcgagg gtcaggaaag
     1201 gttggatatc gtcttcgggc atggttgaaa tcaatgttga cgaagccgct gcgtttcggc
     1261 gagaacgatg ccgccgccga ccagcgcgat ggcgatcaga tggaagcctt ccagccgcgc
     1321 atgcagcagc acgatggact gcaaaatgcc gaaaatcggc agcaggttga tgaacagggc
     1381 ggcgcggttg gcgccgatca gctcgacgcc cgccacccag gtcgcggacg agatcagggc
     1441 gacgaaaacg gcggcgtaaa gcgcgaccag ccagccggtg agatcgggcg cggccagcgg
     1501 cgcactcgcc gcgcgccaga tcaggaacgg aacgcaggtg agcgccgccg ccaggcacgg
     1561 cgcggccaga aagctgcgcc agtgcagcgg cggcttgccc ttcaatccga ccgaatagcc
     1621 cgcatagacc acgaccgcca gcagcatcag cgcgtcgccc gcgttcagtt taaggcgtaa
     1681 caggccctcc gggtggccgt gcgtggcggt gatgaccacg ccgccgaggg tcagggcgcc
     1741 gccgatcagt tgcgccgtcg tcgtcctttg ccgcagcagc gcgaaattgc cggtgaagat
     1801 gacgaacggg atggccgcct gttcgatcat cacattgata acgctcgtct ttgtcagggc
     1861 cgtataaagc aggatgttaa atatggcgaa gccggacgcg ccgtaggcga acagcatgac
     1921 tccatgccgc cggatcaccg gccagtcggc cctgagttgt ttcagcgaca gcgcgacggc
     1981 gacggcggcg gctatgcccc aacgcagcga ggtcagcagg atcgggtcga tatgccccgc
     2041 gccgatcttg
//
LOCUS       DASVLL010000351         5333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_343820,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000351 DASVLL010000000
VERSION     DASVLL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343820"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF402_05845"
     CDS             <1..229
                     /locus_tag="VF402_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799850.1"
                     /translation="LRNGVDASGNASVGADADTGAVTDTAGSAVGDVKNDAATAAHQA
                     GGAVNDALNAATSAKAGVSGSASVGADASSN"
     gene            251..628
                     /locus_tag="VF402_05850"
     CDS             251..628
                     /locus_tag="VF402_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799851.1"
                     /translation="MKKLLLLTGAALMLSGGAALAQNAGTDTGTGASQAQLQREHNRA
                     VKAGDTANDPMNSASSDALNQQQLQGAPIPDTPPAASDAGGPTTTPDATTAPPPVDPN
                     AAPPPAQPPATDSGSSSDSSSSM"
     gene            935..1150
                     /locus_tag="VF402_05855"
     CDS             935..1150
                     /locus_tag="VF402_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799852.1"
                     /translation="MKSILHIAAAAALVTGLAVVPMISAAQDATPSSDATAAAASSSS
                     DSSSTASASADSSSSDSSSSSSSSSSM"
     gene            complement(1232..2188)
                     /gene="era"
                     /locus_tag="VF402_05860"
     CDS             complement(1232..2188)
                     /gene="era"
                     /locus_tag="VF402_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX7799853.1"
                     /translation="MTDTPTSQRCGFVAIIGAPNAGKSTLVNRLVGTKVSIVTQKVQT
                     TRFPVRGIAIDGDVQMVLVDTPGIFKPRRRLDRAMVRSAWSGAEDADAIVLLVDAAAQ
                     VAVMGGGNAPTPADRKAVEDVESIVEGLQKNGAKAILALNKIDLLKRENLLAVADSLF
                     KAGVFSDVLMISADKGQGVADLSRMLAARMPDGPWLYPEDQAADAPVRILAAEITREK
                     LFLRVHEELPYAAAVVTTAFKDMPDGSARIEQTIFVERDGQRAIVLGKGGQTLKWIGQ
                     KAREELSVLLDRRVHLFLHVQVDERWSDDRALYSQFGLDYDS"
     gene            complement(2185..2895)
                     /gene="rnc"
                     /locus_tag="VF402_05865"
     CDS             complement(2185..2895)
                     /gene="rnc"
                     /locus_tag="VF402_05865"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX7799854.1"
                     /translation="MTARAHNQRHLAIDALQDRLGYRFRNPELLELALTHASVAEGAR
                     KMADNERLEFLGDRVLGLMIAEALMEAFPDASEGELSRRFHALVSRETCAGVARAIGL
                     GAALRLAAGETKSGGRDNPTILGDACEALMAALYCDGGYAEVARVFRPLWTQALKDSG
                     DSSRSNPKSYLQEWAAAHGRRAPVYSLIERRGPDHAPSFTMQVAVEGLAPQTAMGRSR
                     QEAEKAAALGLIERDRLS"
     gene            complement(3100..3972)
                     /gene="lepB"
                     /locus_tag="VF402_05870"
     CDS             complement(3100..3972)
                     /gene="lepB"
                     /locus_tag="VF402_05870"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7799855.1"
                     /translation="MTTSDDTTPNTQPQAAAARREPVETAKIVSDKPEPETDNRSARQ
                     VAIDEAKEIVGVILVALALVMILRTLLFQPFTIPSASMEPNLYQGDYIIISKWNYGIS
                     KYSFPLPFPVVKGRWFNHQAERGDVVVFKLPRDPKIDYIKRIIGVPGDVVQMKQDKLY
                     INGKLVPSTQLGPIAAEDGPGGQATEYREALPNGRTHTIQDFFTNGPADDTGEYVVPA
                     GHYFVMGDNRDNSLDSRFSPDDPYEPGVGLVPAENLEGRAVLVLMSWKEGASLWKPWT
                     WLNFHWNRFVKPIH"
     gene            complement(4112..4501)
                     /gene="acpS"
                     /locus_tag="VF402_05875"
     CDS             complement(4112..4501)
                     /gene="acpS"
                     /locus_tag="VF402_05875"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7799856.1"
                     /translation="MILGVGVDLVDGRRIAESIDRFGAKFLDRIFTKDEQAHAVRAAK
                     PHLSYAKRFAAKEAVAKALGTGVRGFLFVDIEVFSDALGKPHVVLHAGAAEVLRGRLP
                     EGKTAHIHLSLSDEDPYATAFATIEIV"
     gene            complement(4498..5247)
                     /locus_tag="VF402_05880"
     CDS             complement(4498..5247)
                     /locus_tag="VF402_05880"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX7799857.1"
                     /translation="MSRIRLGLNVDHVATVRNARGGHAPDPERAARLALQAGVDGITA
                     HLREDRRHILDRDVAALKAVCDAFGKPLNFEMAVTDEMVGVALDLKPHAACLVPERRQ
                     ERTTEGGLDVAGRIETVGAAVAAFNAAGIRASLFIAADPAQVEAAAKIRAPVVEFHTG
                     ALCDAWRDQEDYLFESELNRLQAAAKLADEFGIEVHAGHGIDYDTVPFIAKLPQAREL
                     NIGHFLIGEAIFVGLEAAIQRMRDLMDAARR"
     gene            complement(5252..>5333)
                     /locus_tag="VF402_05885"
     CDS             complement(5252..>5333)
                     /locus_tag="VF402_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7799858.1"
                     /translation="YPADNLPPELAAIPVEDPGSRRLAKA"
BASE COUNT          914 a         1857 c         1655 g          907 t
ORIGIN      
        1 cctcaggaac ggcgtcgacg cctcgggcaa tgcctccgtc ggcgccgatg ccgataccgg
       61 cgccgtcacc gatacggccg gaagcgccgt cggggacgtc aagaacgacg ccgccacggc
      121 cgcgcatcag gcgggcggcg ccgtcaacga cgcgctgaac gccgcgacat ccgccaaggc
      181 cggcgtcagc ggttcggcca gcgtcggcgc ggacgcctca tccaactaaa actcagcagg
      241 agaaccagcg atgaaaaagc ttcttttatt gacaggcgcg gcgctgatgc tcagcggcgg
      301 cgcggcgctg gcccaaaacg ccggaaccga taccggaacc ggcgccagcc aggcccagtt
      361 gcagcgcgag cacaaccgcg cggtcaaggc gggcgacacc gccaacgacc cgatgaattc
      421 ggccagcagc gacgcgctga accagcagca attgcagggc gcgcccattc ccgacacgcc
      481 gcccgccgcc agcgacgcgg gcggccccac cacgacgccg gacgcgacca ccgcgccgcc
      541 accggtcgat cccaacgccg ccccgccgcc ggctcagccg cccgcgaccg attccgggtc
      601 gtcctcggac tccagttcct cgatgtaaac cggcccggcc cgcgaagccc ccacttcgcg
      661 ggccggtttt tcacgcaaat aagccatcgg tctcaatgta tagaaaaata aaaaatccat
      721 aaaaatagta taaatagaga tgacttttgc cgaagcctcc gcattgaaac cctttacaga
      781 ccatagtttc gacacattca aaaaccaacc tgagtatagg tttcgtcgac atttgttact
      841 atgctgacaa actcgaaacg ttgaacgcaa tacttgtcga ttcaacgtga aatctagaaa
      901 agaagtccac ctccaccata gaaagataaa agccatgaag tccatcctgc acatcgccgc
      961 cgccgccgcc ctggtcaccg gtctggccgt cgttccgatg atctcggccg ctcaggacgc
     1021 cacgccttcg tccgacgcca cggccgccgc cgcgtcctcg tccagcgatt cgtccagcac
     1081 ggcgtcggcc tcggcggatt cgtcgtcgtc cgactcgtcg tcgtcctcgt cttcctcttc
     1141 gtcgatgtaa gctccccccc tcttgcatcg tcgaaaaagc ccccggaccg cgaggtccgg
     1201 gggcttatcc atgtcaaccg ccgccattgg ctcagctatc gtaatcgagc ccgaactggc
     1261 tgtagagcgc gcggtcgtcg ctccagcgct cgtcgacctg cacatgcagg aacagatgca
     1321 cgcggcggtc cagcagcacg ctcaactcct cgcgcgcctt ctggccgatc catttcagcg
     1381 tctgcccgcc cttgcccagc acgatggcgc gctggccgtc gcgctcgacg aagatggtct
     1441 gctcgatgcg cgccgagccg tccggcatgt ccttgaacgc cgtggtgacc accgccgccg
     1501 catagggcag ttcctcatgc acgcgcagga acagcttttc gcgcgtgatc tcggcggcca
     1561 gaatacgcac cggcgcatcg gcggcctgat cctcaggata cagccacggc ccgtccggca
     1621 tgcgcgcggc cagcatccgc gacaggtcgg ccacgccctg ccccttgtcc gccgagatca
     1681 tcagcacgtc gctgaacacg cccgccttga acaggctgtc ggcgacggcc agcaggttct
     1741 cgcgcttcag aaggtcgatc ttgttgagcg ccagaatcgc cttggcgccg ttcttttgca
     1801 ggccctcgac aatgctctcg acgtcctcga ccgccttgcg gtcggcgggg gtcggcgcat
     1861 tcccgccgcc catgaccgcc acctgcgccg ccgcgtcgac cagcagcacg atggcgtcgg
     1921 cgtcctcggc cccgctccac gccgagcgca ccatggcgcg gtccagccgg cggcgcggct
     1981 tgaagatgcc cggcgtgtcg accagcacca tctgcacgtc gccgtcgatg gcgatgccgc
     2041 gcaccggaaa gcgcgtcgtc tgcaccttct gcgtcacgat cgacaccttg gtcccgacca
     2101 gccggttgac cagcgtcgat ttgcccgcgt tcggcgcgcc gataatggcc acgaaaccgc
     2161 agcgttgcga ggtaggcgta tctgtcacga caatctgtct ctttcgatca ggcccagcgc
     2221 ggcggccttt tcggcctcct gacgcgagcg tcccatagcg gtctgcggcg ccagtccctc
     2281 gacggcgacc tgcatggtga agctgggcgc gtggtccggc ccgcgccgtt cgatcagcga
     2341 atagaccggc gcgcgcctgc cgtgcgccgc cgcccattcc tgaagatagg atttcggatt
     2401 ggagcgcgag gaatcgccgc tgtccttcag cgcctgcgtc cacaggggcc ggaacacgcg
     2461 cgcgacctcg gcatagccgc cgtcgcagta gagcgcggcc atcagcgcct cgcacgcgtc
     2521 gcccaatatg gtcggattgt cgcgcccgcc gcttttcgtc tcgcccgccg ccagccgcag
     2581 cgcagccccc agcccgatgg cgcgcgccac cccggcgcag gtctcgcggc tgaccagggc
     2641 gtgaaaacgg cgcgacagtt cgccctcgct ggcgtcgggg aacgcctcca tcagcgcctc
     2701 ggcgatcatc agccccagca cgcggtcgcc gagaaactcc agccgctcgt tatcggccat
     2761 cttgcgcgcg ccctcggcca cgctggcgtg cgtcagggcc agctccagca gctccggatt
     2821 ccggaagcgg tagccgagcc ggtcctgaag ggcgtcgatg gccagatgac gttgattgtg
     2881 cgcgcgggcg gtcatccgcc agcgtttaca tcacccgccc tttcagagca aagcctaatg
     2941 ttctcaggaa aaaacggacg gtcccggtaa acccggcgaa tttcgcgcct caaaaggcaa
     3001 aaagacgcgg ctgaaagccg cgtcctacct gtcgccgggt cagtcaaaga agattgcgcg
     3061 cctgagccat gccaaacgcc tttccgcgat tccaacccct caatggatcg gtttgacgaa
     3121 gcggttccag tggaagttca gccacgtcca cggcttccac agggacgcgc cttccttcca
     3181 cgacatcagc accagcacgg cgcggccttc gaggttttcc gcaggcacga ggccgacgcc
     3241 cggctcatac ggatcgtcgg gcgagaagcg gctgtccagc gagttgtcgc ggttgtcgcc
     3301 catgacgaaa taatgtccgg cgggcacgac atattcgccg gtgtcgtcgg cggggccgtt
     3361 ggtgaagaag tcctggatgg tgtgggtgcg cccgttgggc agcgcttccc ggtattcggt
     3421 cgcctgaccg ccggggccgt cctcggcggc gatggggccc agttgcgtgc tggggaccag
     3481 cttgccgttg atgtagagct tgtcctgctt catctgcacg acgtcgcccg gcacgccgat
     3541 gatgcgcttg atgtaatcga ttttcgggtc gcgcggcagc ttgaacacca ccacgtcgcc
     3601 gcgttcggcc tgatggttga accagcgtcc cttgaccacc gggaacggca gcgggaacga
     3661 atatttcgag atgccgtaat tccacttgga gatgatgatg tagtcgccct gatacaggtt
     3721 cggctccatc gaggccgacg ggatggtgaa gggctggaac agaagcgtgc gcaggatcat
     3781 gaccagcgcc agcgccacca ggatcacgcc gacgatctcc ttggcctcgt cgatggcgac
     3841 ctgtctggcg ctgcggttgt cggtttcggg ttcgggcttg tcgctgacga tcttcgctgt
     3901 ttcgacaggt tcgcggcgcg cggcggcggc ctgcggttgc gtgttcggcg tcgtgtcgtc
     3961 ggatgtggtc atcagctttt cgccccgtaa atcccgccgg cgcgcagagt taacgcccgc
     4021 aaagtccgcc ccacctaacc acagaatatg gcgagggcaa gacaattccc cgtacgcgcg
     4081 ggcggcgcgg ggttaaatat ccttcaccca tctagacgat ttcgatggtc gcaaaggcgg
     4141 tggcgtaagg atcctcgtcg ctcagcgaca ggtggatgtg ggcggttttc ccctccggca
     4201 gcctgccgcg cagcacctcg gcggcgcccg cgtgcagcac gacgtgcggc ttgcccagcg
     4261 cgtcggaaaa cacctcgatg tcgacgaaca ggaagccgcg caccccggtg cccagcgcct
     4321 tggcgaccgc ctccttggcc gcgaagcgct tggcgtagct caggtgcggt ttcgccgccc
     4381 tgacggcgtg ggcctgctcg tccttcgtga agatgcggtc gaggaatttc gcgccgaacc
     4441 ggtcgatcga ctcggcgatg cggcgcccgt ccaccagatc gacgccgacg cccaggatca
     4501 tcggcgcgcc gcgtccatca ggtcgcgcat ccgctgaatc gccgcctcca gcccgacgaa
     4561 gatcgcctcg ccgatcagga aatggccgat gttcagttcg cgcgcctgcg gcagtttcgc
     4621 gatgaacggc acggtgtcgt aatcgatgcc gtgtccggcg tggacctcga tcccgaattc
     4681 atccgccagc ttagccgccg cctgcaagcg attgagttcg ctctcgaaaa gatagtcttc
     4741 ctgatcgcgc cacgcatcgc agagcgcgcc ggtgtggaat tcgaccaccg gcgccctgat
     4801 cttcgcggcc gcctcgacct gcgccggatc ggcggcgatg aacagcgacg cgcggatgcc
     4861 cgcggcgttg aacgcggcga cggcggcgcc gacggtctcg atccgcccgg ccacgtccag
     4921 cccgccctcg gtcgtgcgct cctgacggcg ctccggcacc agacaggcgg cgtgcggctt
     4981 gagatcgagc gcgacgccga ccatctcgtc ggtcacggcc atctcgaaat tgagcggctt
     5041 gccgaaggcg tcgcacaccg ctttcagcgc cgcgacgtcg cggtcgagaa tgtggcggcg
     5101 gtcttcgcgc aggtgcgccg tgatcccgtc cacgcccgcc tgcagggcca gccgcgccgc
     5161 gcgttcggga tcgggcgcat gaccgccgcg cgcgttgcgc acggtggcga cgtgatcgac
     5221 gttcagtcct aagcggatgc gggacatgac tttacgcctt ggcaagacgg cggctgccgg
     5281 gatcctcgac cgggatggcg gccagttccg gcggcaggtt gtcggcggga taa
//
LOCUS       DASVLL010000352         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_22175, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000352 DASVLL010000000
VERSION     DASVLL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22175"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..724)
                     /locus_tag="VF402_05890"
     CDS             complement(44..724)
                     /locus_tag="VF402_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX7799859.1"
                     /translation="MMLHVPEVLSKDEVRVLRARLEAAPWVDGRATVGAQGGVVKRNR
                     QVDEHAEAGRELGEVVLAALKRSPLFFAAALPLRIAPPLFNRYEGGETYGFHVDGSVR
                     NLPGGEGKLRTDLSCTLFLCEPEDYDGGVLEVSDTYGVHEAKLPAGDLILYPSSSLHQ
                     VTPVTRGARVCSFFWLQSLVRDETQRRLLFELDATIIRLRAQLGDSAEVLGLTGHYHN
                     LLRMWAEV"
     gene            complement(735..2987)
                     /locus_tag="VF402_05895"
     CDS             complement(735..2987)
                     /locus_tag="VF402_05895"
                     /inference="COORDINATES: protein motif:HMM:NF007349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HEX7799860.1"
                     /translation="MTSVRNRKHALNPYALMLTGAAICVSGAAASLAHADALLADGST
                     EVVVKGEKAPVLKRTAALVDTPQTIEVIKKELIEQQGATTLTEALRNSPGVGTFYLGE
                     NGSTSTGDALYMRGSDVSGSIFVDGVRDVATISRDMFNIESVEVLKGGAGADIGRGAA
                     TGAIDLQSKRPLLDDLYSGSAAVGGGDFYRATADLNWRTGAHSGFRLNLMDQDAGVAG
                     RDEVKNKRWGVAPTLGFGINTPTRVTLGWQHVEQDNVPDGGVFTIGLPGYTSPDGAGS
                     RAFLNSAPKVNPENFYGTKDDHDTATQDLLTAIVEHDFRDGWRLSNITRYGKTDEDYQ
                     LSSFMGQAATGTRPLLTPNPDDPATWTLTRLVNNRNATNETLVNHTNLAGQFDTGFVH
                     HSVSAGLEFSREEQTTRAFDTSAAAGAYPAVSVYHPDPNVGGYTRRLSGAYTDGVTDT
                     QAAYVNDTLSLTPRVLINAGLRYDNFQTDYSSIAADGSNAAAPLHIRDGVWSGRLGLI
                     YKPAKNGSLYASYAETTQPPGGANFSLSATAGNANNPDIKPQVARNWEAGTKWDLAHG
                     HLSLTGAVYRTQYSDTVTQDTDGSYYHTGSKSAEGIELGAVGHITREWTVNAGYMINH
                     TKVKDAAVVTSDGSTDLAYSPSDAFTLWTAYEAHHGRFHGLTIAGGPRYNGEMKRGKD
                     GAVGTPAYVKSYWTMDGMASYPLTRHVVAQLNIYNLFDADYVAAINKSGYRYTPGTPR
                     TVRLTLNFKY"
     gene            3217..>3360
                     /locus_tag="VF402_05900"
     CDS             3217..>3360
                     /locus_tag="VF402_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEX7799861.1"
                     /translation="MTLRDLPLDTPALVTQVRDPSGSDIISQRLRELGFVPGEPVEVV
                     GRGP"
BASE COUNT          521 a         1103 c         1118 g          618 t
ORIGIN      
        1 aagacttggg gccaactggc cccaaacccc gtaacggtcg gcgttatacc tccgcccaca
       61 tccgcagcag attgtggtag tgcccggtca ggcccagcac ttcggcgctg tcgcccagtt
      121 gcgcgcgcag gcggatgatc gtggcgtcca gctcgaacag cagccgccgc tgcgtctcgt
      181 cgcgcaccag actttgcagc cagaagaacg agcagacccg cgcgccgcgc gtcaccggcg
      241 tgacctgatg caggctcgac gacggataga ggatcaggtc gcccgccggc agcttggcct
      301 catgcacgcc gtaggtgtcg gacacttcca gcacgccgcc gtcataatcc tccggctcgc
      361 acagaaacag ggtgcaggac aggtcggtgc gcagcttgcc ctcccccccc ggcaggttgc
      421 gcaccgagcc gtcgacgtga aagccgtagg tctcgccgcc ctcgtaacgg ttgaacaggg
      481 gcggcgcgat gcgcaaaggc agggcggcgg cgaagaacag cggactgcgc ttcaacgccg
      541 ccagcacgac ctcgcccagt tcgcgcccgg cctcggcgtg ttcgtcgacc tgtcggttgc
      601 gcttgaccac cccgccctgc gcgccgaccg tcgcccggcc gtcgacccag ggcgccgctt
      661 cgagacgcgc gcgcaggacg cggacctcgt ccttggacag cacctccggg acatgcagca
      721 tcatgggcgt ttcactagta tttgaagtta agcgtcaggc gcaccgtgcg cggcgttccc
      781 ggcgtgtaac gatagccgct cttgttgatc gccgccacat aatccgcgtc gaacaggttg
      841 tagatgttga gctgcgcgac gacatggcgg gtcagcggat agctggccat accgtccatc
      901 gtccagtagg acttgacgta ggcgggcgtg ccgaccgcgc cgtccttgcc gcgcttcatc
      961 tcgccgttat agcgcgggcc gcccgcgatc gtcaggccgt ggaagcgccc gtgatgcgct
     1021 tcgtaagccg tccacagcgt gaaggcgtcg ctggggctgt aggccagatc ggtcgagccg
     1081 tccgaggtca ccaccgcggc gtccttcacc ttggtgtggt tgatcatata gcccgcgttg
     1141 accgtccatt cgcgcgtgat gtggccgacc gcgcccagct cgatcccttc ggcggacttc
     1201 gagccggtgt ggtaatagga gccgtccgtg tcctgcgtga cggtgtcgga atactgcgtg
     1261 cgatagaccg cgccggtcag cgacaggtgg ccgtgcgcca gatcccactt cgtccccgct
     1321 tcccagttgc gcgcgacctg cggcttgatg tcggggttgt tggcgttgcc cgccgtcgcc
     1381 gacagcgaga agttggcccc gcccggcggt tgagtggttt cggcgtagga ggcgtagagg
     1441 ctgccgttct tcgcgggctt gtagatcagg cccagacggc cgctccacac cccgtccctg
     1501 atgtgcagcg gcgcggcggc gttcgatccg tcggcggcga tggagctgta gtcggtctgg
     1561 aaattgtcgt aacgcagacc ggcgttgatc agcacccgcg gcgtcaggct cagggtgtcg
     1621 ttgacatagg ccgcctgcgt gtcggtgacg ccgtccgtat aggcgccgct caggcggcgg
     1681 gtatagccgc cgacgttcgg atcgggatga tagacgctga ccgccggata ggcgcccgcg
     1741 gcggcggacg tgtcgaaggc gcgcgtcgtc tgctcctcgc ggctgaactc aagccccgcg
     1801 ctcacactgt gatgcacgaa gccggtgtcg aactgccccg ccagattggt gtggttgacc
     1861 agggtttcgt tggtggcgtt gcgattgttg accaggcgcg tcagcgtcca ggtcgccgga
     1921 tcgtccgggt tcggcgtcag caagggacgc gtgccggtcg ccgcctgccc catgaaggag
     1981 gaaagctggt agtcctcgtc ggtcttaccg tagcgcgtga tgttgctcag gcgccagcca
     2041 tcgcggaaat catgctcgac aatggcggtc aggaggtcct gggtcgcggt gtcgtgatcg
     2101 tccttcgtcc cgtagaaatt ctcggggttc accttcggcg cgctgttcag gaaagcgcgc
     2161 gaaccggcgc cgtccggcga ggtatagccg ggcagaccga tggtgaagac gccgccgtcg
     2221 ggcacgttgt cctgctcgac atgctgccag cccagggtga cgcgcgtcgg cgtgttgatg
     2281 ccgaagccca gcgtcggcgc cacgccccag cgcttgttct tcacctcgtc gcggcccgcc
     2341 acgcccgcgt cctgatccat caggttcagg cggaagccgc tgtgcgcgcc ggtgcgccag
     2401 ttcaggtccg ccgtggcgcg gtagaagtcg ccgccgccga ccgccgccga gccggaatag
     2461 aggtcgtcga gcagcggacg cttgctttgc aggtcgatgg cgccggtcgc cgcgccgcgc
     2521 ccgatgtccg cgcccgcgcc gcccttcagc acctcgaccg actcgatgtt gaacatgtcg
     2581 cgcgaaatcg tcgccacgtc gcgcacgccg tcgacgaaga tgctgcccga cacgtccgag
     2641 ccgcgcatgt aaagcgcgtc gcccgtcgag gtgctgccgt tttcgccgag atagaaggtt
     2701 ccgacgcccg gtgaattgcg cagcgcctcg gtcagggtgg tcgcgccctg ctgctcgatc
     2761 agttccttct tgatcacctc gatggtctgg ggcgtgtcga ccagcgccgc cgtgcgcttc
     2821 agcaccggcg ccttctcgcc cttgaccacc acttcggtgg acccgtcggc cagcagggcg
     2881 tcggcgtgcg ccagcgacgc cgccgccccg gaaacgcata tggccgcgcc ggtcagcatc
     2941 agggcgtagg ggttgagagc gtgtttgcga ttacgaacag aggtcatggc tttgagttac
     3001 cgtttcgatt atgaaatctt gtggccccgg tcatattgag aatcattctc acttgcaagc
     3061 ggattttgcg aataatttgc atattcattc gcaagcgcct atatgacgac aggccgcagt
     3121 ttggaaaccg gctgtgactt gacccgcgcg cgaccgtgaa cgataagcgc agatcatagt
     3181 gataatcatt cgcaagcctt gttgagtgcc ccgttgatga ccctgcgcga cctgccgctc
     3241 gatacgcccg ccctggtgac tcaggtgcgc gacccgtccg ggagcgacat catcagccag
     3301 cgcctgcgcg aactgggttt cgtgccgggc gagccggtcg aggtcgtggg gcgcggcccg
//
LOCUS       DASVLL010000353         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_343919,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000353 DASVLL010000000
VERSION     DASVLL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343919"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..910
                     /locus_tag="VF402_05905"
     CDS             287..910
                     /locus_tag="VF402_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7799862.1"
                     /translation="MSSGARARSKERNRLKILDSATRMFRERGFEAATLRDIASAAGL
                     STGALFANFTDKTEIFLTVVESEHQRVLAAMRESYDEGLPLVERLHRQLMRGYEVSEA
                     NARLILSAFVMKWSADQTTLNDVARLSDDIRDALFDTLTRAQARGELPGDAPLQDASE
                     VLEDLCFANMRRAFHKAGEAGGFDAATLERNMLTQLKLVIAGLKAGA"
     gene            1103..>2049
                     /locus_tag="VF402_05910"
     CDS             1103..>2049
                     /locus_tag="VF402_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7799863.1"
                     /translation="MFFQRSNEGDKAVVAALNASQAVIEFTPGGDILTANDNFLACVG
                     YRLDEIAGRHHSLLCDPDHVASDAYRGFWRDLAAGQFRSDVFKRIGKGGRVVWLQASY
                     NPIRDKTGKVVRVIKFASDVTEQKRREMDAQGKIDAIKRSQAVIEFTPTGEVLTANDN
                     FLNALGYRLDEIEGRHHSLFCDPDYVATDAYRDFWARLGRGEFQSGEFRRLGKGGRDV
                     WIQAIYSPILDEDGKVVRVVKFATDVTKMVLRRQGNERATDSFGHVVGQIGAAREKTV
                     IASSASNETGAIINSVAAAAEELSQSVREIAENMSSARV"
BASE COUNT          385 a          678 c          644 g          342 t
ORIGIN      
        1 cggccagcat cgcgatcagc gccagaatga aaagaaaaag gctcataagg tcagtttccg
       61 ccttttaaag gcgtctgtcg agcgtgcgcg ctaacaattt ttataccgcg ttgcggtaaa
      121 tcccgcccgc tgcaacgcaa aaaaaaccat atcgctttgt tgtattgcag cccccgctgt
      181 actaagtaac gtgcgccggg ggaccaagct gacgcttggg tcaacttttt gtgtgagcgt
      241 aatcatgttc gatcccagtc ttcctacccc cgcccaggag tcgcccgtgt cgtcaggagc
      301 ccgcgcccgt tcaaaagagc gcaaccgcct gaaaatcctc gactcggcga cccggatgtt
      361 ccgcgaacgc ggtttcgagg cggccacctt gcgcgatatc gccagcgcgg cgggcctgtc
      421 caccggcgcc ctgttcgcca acttcaccga caagaccgaa atcttcctga ccgtggtcga
      481 gtccgagcat cagcgcgttc tggccgccat gcgcgaatcg tatgacgagg gcctgccgct
      541 ggtcgagcgc ctgcaccgcc agctcatgcg cggctatgag gtcagcgagg ccaatgcccg
      601 cctgatcctg tcggccttcg tgatgaagtg gagcgccgac cagaccacgc tcaacgacgt
      661 cgcgcgcctc agcgacgata tccgcgacgc cctgttcgac accctgaccc gcgcgcaggc
      721 gcgcggcgaa ctgcccgggg acgcgccgtt gcaggacgct tcggaagtgc tggaggatct
      781 gtgcttcgcc aatatgcgcc gcgccttcca caaggcgggc gaggcgggcg gcttcgacgc
      841 cgcgacgctg gagcgcaaca tgctgaccca gctcaagctg gtgatcgcgg gcctgaaggc
      901 cggggcgtaa gcggcgcaag ccttccgggc gcgccgcgcc gcacgcccgg acgcaaccat
      961 aacgattgtt cttccaaaat gtgcgtttac ggtaaacgcc ttcttaagac atcgcgccgc
     1021 atagtcgcgg cgtgaagaac aagggggttc gccgaccggg cgcatgggcc gcgccgggtc
     1081 ctgtcttcag gagggtgcgc gtatgttttt ccaacggtcc aatgaggggg acaaggccgt
     1141 cgtcgcggcc ctgaacgctt cgcaggcggt gatcgagttc acgcccggcg gcgatatcct
     1201 caccgccaac gacaattttc tcgcctgcgt cggctaccgg ctggacgaga tcgccggacg
     1261 tcatcacagc ctgctctgcg atcccgacca tgtcgcctcg gacgcctatc gcggcttctg
     1321 gcgcgatctg gcggcgggac agttccgcag cgacgttttc aaacgcatcg gcaagggcgg
     1381 ccgcgtcgtc tggctgcagg ccagctacaa tccgatccgc gacaagaccg gcaaggtcgt
     1441 gcgcgtgatc aagttcgcca gcgacgtcac cgaacagaag cggcgcgaga tggacgcgca
     1501 gggcaagatc gacgccatca agcgctcgca ggcggtgatc gaattcacgc cgaccggcga
     1561 ggtgctgacc gccaacgaca atttcctgaa cgcgctcggc tatcgcctcg acgagatcga
     1621 gggccgccac cacagcctgt tctgcgatcc cgactatgtc gccacggacg cctatcgcga
     1681 cttctgggcg cggctggggc gcggcgagtt ccagtcgggc gagttccgcc gcctcggcaa
     1741 gggcgggcgc gacgtctgga ttcaggcgat ctacagcccg atcctcgacg aagacggcaa
     1801 ggtggtccgg gtggtcaagt tcgccaccga cgtgaccaaa atggtgctgc gccgtcaggg
     1861 caacgagcgc gccaccgaca gcttcggcca cgtggtcggc cagatcggcg cggcgcgcga
     1921 aaagaccgtc atcgcctcca gcgcctccaa cgaaaccggc gccatcatca attcggtcgc
     1981 cgccgccgcc gaggagctgt cgcaaagtgt gcgcgagatc gccgagaaca tgtcgagcgc
     2041 gcgcgtcag
//
LOCUS       DASVLL010000354         4430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_183685,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000354 DASVLL010000000
VERSION     DASVLL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183685"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VF402_05915"
     CDS             <1..601
                     /locus_tag="VF402_05915"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX7799864.1"
                     /translation="SETFSRRNINCSITESLKRFRPLCAAAARDGVRVRGYASCAIAC
                     PYEGPVAPEAVVRVAEALFDMGCYEVSLGDTIGAGGPNDAQRLLDAVLRRIPADRIAV
                     HFHDTYGQALANILTSLDYGVAVIDSSVGGLGGCPYAPGAAGNVATEDVLYMLDRMGI
                     ETGVDLSAVVDAAWFIADVLGRPPRSKVALAMTPRNAAR"
     gene            complement(573..2069)
                     /locus_tag="VF402_05920"
     CDS             complement(573..2069)
                     /locus_tag="VF402_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7799865.1"
                     /translation="MGGKIQSLVVLGGGTAGWMAAAMLARSWGERLRVTLIESDAIGT
                     VGVGEATIPPLIQLLRFLGLDEDVVLRNIQGTFKLGIEFDGWRREGERYMHAFGSPGS
                     AIGVTPFYQYWLRRHLNGEDCGSLWDYSLNARMAYAGKMSREARVEGNLAGPDRAFHF
                     DAGLLARFLRRGCEASGVIRREGKVTGVSLRSDDGFIESLTLDSGEVVAGDMFIDCSG
                     FGGVLIEGALKAGYEDWSHWLPCDRAVTAATAPNGPPAPYTCTRAHAAGWQWRIPLQH
                     RTGNGHVFCSAMMDNDAATRILLDHLDGAPLNEPRVIGFVTGRRKSTWVKNCVALGLA
                     SGFMEPLESTSIHLVQSVMTRFLEFFPDADFNPVEIAEFNRRTRREYELIRDFLVLHY
                     HANGRDEPFWRERRAAAIPDSLAHKLDYFRQHGRLLVEEGDLFREASWAQVLIGQGVL
                     PAAASPMTGTVGAEALSAFMSGLKETQARAMAAMPSHADFIARHCAAS"
     gene            complement(2069..2551)
                     /locus_tag="VF402_05925"
     CDS             complement(2069..2551)
                     /locus_tag="VF402_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7799866.1"
                     /translation="MVLVVDDNHYMRVIVSTMLRSMGINLIREASDGIDALEIVRDWR
                     PDVVIIDLMMEQLDGVEFTKLVRRGVDSPNPYVPIIMMTGHTDRRHVIDARDAGVSEF
                     IAKPLTARALIDRLKSVINNERGWVKSATYVGPDRRRRSVPDYKGPFRRASDKADGKF
                     "
     gene            2703..>4430
                     /locus_tag="VF402_05930"
     CDS             2703..>4430
                     /locus_tag="VF402_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX7799867.1"
                     /translation="MINWGDKTLIAAAAAVLIAGFGVGFLTDHALSGGKVGKPGAAAG
                     ATADGDPLTPYTGLFGKPRDKNAPRAGDAKPAGFAVWKQNLDTSGNTPKACVEMSRPL
                     DPSKPYGDYVVVSPALPSAPAISVKDDTLCVSGFGFSDRRVTLLKGLPAKNGDTLAAD
                     ADLDFSVGTKPPYVGFSGNGVVLPRADGDGVAIETVNVSSLAVEVWRVSDRNLVRKSV
                     SAPDPVAEGEYDYAYGDDSADDIGVKIWTGKLPVKMQNGERVTTVFPLGAVLKTLQPG
                     AYIVKARDASGGRDKKPDDQPAQARRWILYTDMAMMTYKGPNGLDVVMRSLKTAKPMP
                     GVRVDLVATNGEALGTATTDADGHAAFAGALLKGTDALTAKMVMAYGPNNDYTASDIT
                     RAPMDLSSHAIGGRQEGESGDGRASAFGVDSYVYTDRGIYRPGETVHLVALMRDPQGR
                     VIKDRKGALVVYRPSGVEAFRYAFAATPQGFAGKNITLPKSAPRGQWTAKVLLDGMDD
                     PAGTASFAVEDFAPQRLGVDIDAGPNQPITGLDQTRPVTVSAHYLYGAIGSGLQVTGE
                     ARISADPTPF"
BASE COUNT          745 a         1512 c         1473 g          700 t
ORIGIN      
        1 gtccgagacc ttcagccgcc ggaacatcaa ctgctcgatc acggaaagcc tcaagcgttt
       61 ccgcccgctc tgcgcggcgg cggcgcgcga cggcgtgcgc gtgcgcggct atgcatcctg
      121 cgccatcgcc tgcccgtatg aggggccggt tgcgccggag gcggtcgtgc gcgtggccga
      181 ggccctgttc gacatgggtt gctatgaggt cagtctcggc gacaccatcg gcgcaggcgg
      241 gccgaacgat gcgcaaaggc tgctcgacgc cgtgctgcgg cggattccgg ccgacaggat
      301 cgcggtgcat tttcacgaca cctacggtca ggcgctggcc aatatcctga ccagcctcga
      361 ttacggcgtc gctgtaatcg attcgtccgt aggcggattg ggcggctgtc cctacgcgcc
      421 cggcgcggcg ggcaatgtcg cgactgagga cgtcctgtac atgctggacc ggatgggcat
      481 cgaaaccggc gtcgacctga gcgccgtcgt cgacgcggcg tggttcatcg cggacgtgct
      541 gggccgtccg ccgcgctcga aggtcgcgct ggctatgacg ccgcgcaatg ccgcgcgatg
      601 aagtcggcgt gcgacggcat ggccgccatc gcgcgcgcct gcgtctcttt caggccgctc
      661 atgaaggcgc tcagcgcctc ggcgccgacg gttccggtca tgggcgaggc ggcggcgggc
      721 aggacgccct gaccgatcag cacctgcgcc caactggcct cgcggaacag atcgccctcc
      781 tcgaccagca gccgcccgtg ctgcctgaaa tagtcgagct tgtgcgccag cgagtcgggg
      841 atggccgccg cccggcgctc gcgccagaac ggctcgtcgc gcccgttggc gtgataatgc
      901 aggaccagaa agtcgcggat cagctcgtat tcgcggcggg tgcggcggtt gaattcggcg
      961 atctcgaccg gattgaagtc cgcgtcgggg aagaattcga gaaagcgcgt catcaccgac
     1021 tgcacgaggt ggatcgaggt cgattccagc ggctccatga agccggacgc cagccccagc
     1081 gccacgcagt tcttcaccca ggtcgatttg cgccgcccgg tgacgaagcc gatcacgcgc
     1141 ggctcgttca gcggcgcgcc gtcgagatgg tcgagcagaa tgcgcgtcgc ggcgtcattg
     1201 tccatcatcg cggagcaaaa gacgtggccg ttgccggtgc ggtgctgcaa cgggatgcgc
     1261 cattgccagc ccgccgcatg cgccctggtg caggtatagg gcgcgggcgg cccgttcggc
     1321 gcggtggcgg cggtcaccgc ccggtcgcac ggcagccagt gcgaccagtc ctcgtaaccg
     1381 gccttgagcg cgccctcgat cagcacgccg ccgaagcccg aacagtcgat gaacatgtcg
     1441 cccgcgacga cctcgccgct gtccagggtc agcgactcga tgaagccgtc atcgctgcgt
     1501 aaggagacgc ccgtaacctt gccctcgcgc cggatcacgc ccgacgcctc gcagccgcgg
     1561 cgcaggaagc gcgccagcag cccggcgtcg aaatgaaagg cgcggtccgg cccggccaga
     1621 ttgccctcga cgcgggcctc gcgcgacatc ttgcccgcat acgccatgcg cgcgttgagg
     1681 ctgtagtccc acagggagcc gcagtcttcg ccattaagat ggcggcgcag ccagtactga
     1741 taaaacggcg tgaccccgat cgccgagccg ggcgagccga aggcgtgcat gtagcgctcg
     1801 ccttcgcgcc gccagccgtc gaattcgatg cccagcttga acgtgccctg aatattgcgc
     1861 aggaccacgt cctcatcgag gccgagaaag cgcagaagct ggatcagcgg cggaatcgtc
     1921 gcctcgccga ccccgaccgt gccgatggcg tcggactcga tcagggtgac gcgcaaccgc
     1981 tcgccccaac tgcgcgccag catggccgcc gccatccatc ccgccgtgcc gccgcccaga
     2041 acgaccagcg attgaatctt cccgcccatc agaacttgcc gtccgccttg tcggaggcgc
     2101 ggcggaacgg ccccttgtag tcggggacgc tgcggcggcg gcggtcgggg ccgacatagg
     2161 tggccgactt gacccagccg cgttcgttgt tgatcacgct tttcagccgg tcgatcagcg
     2221 cgcgcgcggt cagcggcttg gcgatgaatt cgctcacccc cgcgtcgcgc gcgtcgatga
     2281 cgtggcggcg gtcggtgtgg ccggtcatca tgatgatcgg cacgtaaggg ttggggctgt
     2341 cgacgccgcg ccgcaccagc ttggtgaact ccacgccgtc aagctgctcc atcatcaggt
     2401 cgatgatcac cacatcgggc cgccagtcgc gcacgatctc cagcgcatcg atgccgtcgg
     2461 aggcctcgcg gatcagattg atgcccatag agcgcagcat ggtggagacg atcacccgca
     2521 tatagtggtt gtcgtcgacg accagcacca tgactcgatc gaacggattg gccaaacgct
     2581 gtgtactccc tgcccagata tatgaatcac tacccccgtt ctgtaaattt ccaaaacaaa
     2641 gattgatctt tgctgaattc cacgtttgtc tttgcgcggg atgaaggccg gggagttcgg
     2701 acatgatcaa ctggggcgac aagacgctga tcgcggcggc ggcggcggtg ctgatcgcgg
     2761 gcttcggcgt cggattcctg accgaccacg cgctcagcgg cggcaaggtc ggcaagcccg
     2821 gcgcggcggc cggcgcgacc gcggacggcg atccgctcac cccctatacc ggcctgttcg
     2881 gcaagccgcg cgacaagaac gctccgcgcg cgggcgatgc gaaaccggcg ggcttcgcgg
     2941 tctggaagca gaacctcgac acgtcgggaa atacgccaaa agcctgcgtc gagatgtcac
     3001 gcccgctcga tccgtccaag ccttacggcg actatgtcgt cgtctcgccc gccctgccgt
     3061 cggcgcccgc catttcggtc aaggacgaca cattgtgcgt gtccggcttc ggcttttccg
     3121 accgccgcgt caccctgctg aaagggctgc ccgcgaagaa cggcgacacg ctggccgccg
     3181 acgccgatct cgatttcagc gtcggaacca agccgcccta tgtcggcttt tcgggcaacg
     3241 gcgtggtgct gccgcgcgcg gacggcgacg gcgtggccat cgagacggtc aatgtctcgt
     3301 cgctggccgt cgaggtctgg cgcgtctccg accgcaatct ggtgcgcaaa tcggtgtcgg
     3361 cgcccgaccc ggtcgccgag ggcgaatacg attacgctta cggcgacgat tccgccgacg
     3421 atatcggcgt caagatctgg accggcaagc tgccggtgaa gatgcagaac ggtgagcgcg
     3481 tcaccacggt ctttccgctc ggcgcggtgc tgaagacgct gcaaccgggc gcctatatcg
     3541 tcaaggcgcg cgacgcaagc ggcgggcgcg acaagaagcc ggacgaccag cccgcccagg
     3601 cgcggcgctg gatcctctac accgacatgg cgatgatgac ctataagggg ccgaacggcc
     3661 tcgacgtggt gatgcgctcg ctgaaaaccg ccaaaccgat gccgggagtc cgcgtcgatc
     3721 tggtcgcgac gaacggcgag gcgctgggca cggcgaccac cgacgccgac ggccacgccg
     3781 ccttcgcggg cgcgctgctg aaagggaccg acgccctgac ggccaagatg gtcatggcct
     3841 acgggccgaa caacgattac accgcctccg acattacgcg cgcgccgatg gacctgtcct
     3901 cgcacgccat cggcggtcgg caggagggcg agagcggcga cggcagggcc agcgctttcg
     3961 gcgtcgattc ctacgtctat accgaccgcg gcatctatcg accgggcgaa accgtgcatc
     4021 tggtcgccct gatgcgcgac ccgcagggcc gcgtgatcaa ggaccgcaag ggcgccctgg
     4081 tcgtctatcg cccgtccggc gtcgaggctt tccgctacgc cttcgccgcc acgccgcagg
     4141 gtttcgcggg caagaacatc accctgccga aatcggcgcc gcgcggccag tggaccgcca
     4201 aggtgctgct cgacggcatg gacgatccgg cggggacggc gtcgttcgcg gtcgaggatt
     4261 tcgcgccgca acgcctcggc gtcgatatcg acgcaggtcc aaaccagccg atcaccggcc
     4321 tcgaccagac ccgtccggtc acggtcagcg cccactatct ctacggcgcc atcggctccg
     4381 gccttcaggt caccggcgag gcgcgcatta gcgccgatcc gacgccgttc
//
LOCUS       DASVLL010000355         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_184003,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000355 DASVLL010000000
VERSION     DASVLL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184003"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VF402_05935"
     CDS             <1..644
                     /locus_tag="VF402_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7799868.1"
                     /translation="TPDRELPFAGHPTLGTAQVWLEDGGRPRAAGEIVQQCGAGLVRV
                     RRDGDRLAFAAPPLLRSGPVAEADLADVLAVLQIGRDAVQAAQWIDNGPGWLGVRLAS
                     AEAVRALRPLGAYHKRMDIGVLGLETGGETAMEVRAFFSDPSGAIREDPVCGSLNASA
                     AQWLLGAGLLTAPYVAAQGTCLGRAGRVHVAQDDGQIWVGGAVRVLFSGTASA"
     gene            737..1366
                     /locus_tag="VF402_05940"
     CDS             737..1366
                     /locus_tag="VF402_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEX7799869.1"
                     /translation="MDLSLPTTLKRLFVTEVYAVGVAGSPAGDALIQRLDAACRRLAR
                     EDKDGRDWAARKRYIGYTSFGSVRDLTQADAAFADLKTLLDQHVVSYTRQVEMSLSKP
                     LSLQSYWVNVLDPLGGHSGHTHPGCVVSGTFYVAMPPDSGGLLFEDPRLPLMMNAPGR
                     QAHARPDRQISASVVPAPGTLLLWESWLRHEVPVNLSQDQRISISFNYA"
     gene            complement(1357..1845)
                     /locus_tag="VF402_05945"
     CDS             complement(1357..1845)
                     /locus_tag="VF402_05945"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX7799870.1"
                     /translation="MNSSDGPVGYRPNVGLVVFNAAGQVWLGHRFGMAGDYAWQFPQG
                     GVDDGEDLEAAARRELYEETGMRSVEILGRTTDWIVYDFPPEVLAQKKIGRNFKGQKQ
                     IWFALRFTGADSEIDLQTHGEQEFSRWEWCDLNSVLSRVVHFKRGSYEQVIAQFAKYV
                     QA"
     gene            complement(1892..3091)
                     /locus_tag="VF402_05950"
     CDS             complement(1892..3091)
                     /locus_tag="VF402_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX7799871.1"
                     /translation="MFAKPKNPSASPAPRRRSGARFDARALGDKVLAVFRRPYVAPVS
                     AAAALVLGVIVFLNVAGDPDAASPSVRIHLDKPKAAAPSSNIAPGDQPQNFALDSLGM
                     FGDASADAFMSANPAQGDAAVITLPGDDSGPAPAAAPKQPASPLAVAPLPSLVQTTPN
                     GPLPIIAPNGVTPASAYARPFHNDGRPMVALIVGGLGLNPAMTKQAISELPPQVTLSF
                     VPYANGLQGWIDLARASGHEVMIETPMQPSDYPDNDPGPQTLMANARPDDLLSHLNWT
                     LSRATGYFALTNYQGSAFFKDRAGVGIFMNTLKQRGVDFIDDGQARNLGGAWGRASAD
                     RIIDNQLNATAIAAQLSGLEATAKSRGQALGTGFAYPVTVAVAIQWTQGLNARGLQLA
                     PASALTH"
     gene            complement(3278..>3484)
                     /locus_tag="VF402_05955"
     CDS             complement(3278..>3484)
                     /locus_tag="VF402_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7799872.1"
                     /translation="NVAEVRAHPKGVVLLTADLADKPGSRFAKLTKPGADGASSSSAA
                     SASSATSSSSGGAASSSGASDSSK"
BASE COUNT          525 a         1113 c         1264 g          582 t
ORIGIN      
        1 ttacgccgga tcgcgaattg ccgttcgcgg gccatccgac gctggggacg gcgcaggtct
       61 ggctggagga cggcgggcgg cccagggcgg cgggcgagat cgtgcagcag tgcggcgccg
      121 gtctggtgcg ggtgcgccgc gatggggaca ggctggcctt cgccgcgccg ccgctgctgc
      181 gttccgggcc ggtcgccgag gccgatctgg ccgacgttct ggccgtgctg caaatcggac
      241 gcgacgcggt gcaggcggcg caatggatcg ataacggccc cggctggctt ggcgtgcggc
      301 tggcctcggc cgaggcggtg cgcgcgctca ggccgctggg cgcttatcac aagcgcatgg
      361 acatcggcgt gctggggctt gagacgggcg gcgagacggc gatggaggtg cgcgcctttt
      421 tcagcgaccc gagcggcgcc atccgcgaag acccggtgtg cggcagcctc aacgcctcgg
      481 cggcgcaatg gctgctgggc gcgggcctgc tcacggcccc ctatgtcgcc gcgcagggga
      541 cgtgtctggg ccgcgcgggc cgggtgcatg tcgcgcagga cgacggccag atctgggtcg
      601 gcggcgcggt ccgcgtcctg ttctccggga ccgcgtcggc ctgaggattc gtatcgaaaa
      661 agaaatgtcg ccaaggcaga cggcgacgac cgtttgcgcc gttaaacgcg ggttcctccc
      721 cggagttgcg cgtcgcatgg acctgagttt gcccaccacc ttgaagcgtt tgttcgtcac
      781 cgaggtttac gccgtcggcg tcgccggatc gcctgcgggc gatgcgttga tacagcggct
      841 ggacgcggcg tgccggcggc tggcgcgcga ggacaaggac ggacgcgact gggcggcgcg
      901 caaacgctat atcggctata cctcctttgg ctcggtgcgc gacctgacgc aggcggacgc
      961 cgccttcgcc gacctgaaaa ccctgctgga tcagcatgtc gtgagctata cgcggcaggt
     1021 cgagatgagc ctgagcaagc cgctgagcct gcaatcctac tgggtcaacg tgctggaccc
     1081 gctgggcggc cattccggcc atacccatcc cggctgcgtg gtgtcgggaa ccttctatgt
     1141 cgccatgccg ccggattcgg gcggcctttt gttcgaggat ccgcgcctgc cgctgatgat
     1201 gaacgcgccg ggccgtcagg cgcacgcccg gcccgaccgc cagatctcgg cctcggtcgt
     1261 gcccgcgccc ggaaccctgc tgctgtggga aagctggctg cgccacgaag tgccggtcaa
     1321 cctgtcgcag gaccagcgga tttcgatcag cttcaactac gcctgaacat atttcgcgaa
     1381 ctgcgcgatc acctgttcgt agctgccgcg cttgaaatgc acgacgcggc tgagcacgct
     1441 gttcaggtcg caccattccc agcgtgagaa ttcctgctct ccgtgggttt gcaggtcgat
     1501 ctcgctgtcc gcgccggtga agcgcagggc gaaccagatc tgcttctggc ccttgaaatt
     1561 gcggccgatc ttcttttgcg ccagcacctc gggcgggaag tcatagacga tccagtccgt
     1621 cgtacggccc agaatctcga ccgagcgcat gccggtttcc tcataaagct cgcgccgggc
     1681 ggcggcctca aggtcttcgc cgtcgtcgac cccgccttgc ggaaactgcc acgcataatc
     1741 gcccgccatg ccgaagcggt ggccgagcca gacctgaccg gcggcgttga agacgacgag
     1801 gccgacattg gggcggtagc cgacgggacc atctgaactg ttcatatggc ttggttacgc
     1861 cggtgttggc gtgcgcgtca agcgtctaaa atcaatgcgt tagcgccgag gcgggcgcca
     1921 attgcagccc ccttgcgttc agcccctgcg tccactggat cgcgacggcg acggtgaccg
     1981 gataggcgaa gcccgtcccc agcgcctgcc cgcggctttt ggcggtcgcc tccagcccgc
     2041 tcagttgcgc ggcgatggcg gtggcgttca actggttgtc gatgatgcgg tccgccgagg
     2101 cgcggcccca ggcgccgccg agattgcgcg cctggccgtc gtcgatgaaa tcgacgccgc
     2161 gctgtttcag cgtgttcatg aagatgccga cgcccgcgcg gtccttgaag aaggcgctgc
     2221 cctgatagtt ggtcagggcg aaatagccgg tggcgcgcga cagggtccag ttcaggtggc
     2281 tgagcaggtc gtcgggccgg gcgttggcca tcagggtctg cggccccgga tcgttgtcgg
     2341 gataatcgct cggctgcatc ggcgtctcga tcatcacctc gtggccgctg gcgcgcgcca
     2401 gatcgatcca gccttgcagg ccgttggcgt aggggacgaa ggacagggtg acctgcggcg
     2461 gcagctcgct gatcgcctgc ttggtcatgg cgggattcag ccccagcccg ccgacgatga
     2521 gcgcgaccat cgggcggccg tcattatgga acggacgcgc ataggccgag gccggggtga
     2581 cgccgttggg cgcgatgatc ggcagcggac cgttcggcgt ggtctgcacc agactgggca
     2641 gcggcgcgac ggccaggggc gaggcgggct gtttcggcgc ggcggcgggc gccgggccgc
     2701 tgtcgtcgcc gggcagggtg atcaccgccg cgtcgccctg agccggattg gcgctcatga
     2761 aggcgtcggc ggaggcgtcg ccgaacatgc cgagcgaatc gagcgcgaaa ttctgcggct
     2821 ggtcgccggg cgcgatgttg gaggacggcg cggcggcttt gggcttgtcg agatggatgc
     2881 gcacgctggg cgaggccgcg tcgggatcgc ccgccacgtt gagaaagacg atcacgccca
     2941 gaaccagcgc cgccgccgcg ctgacaggcg cgacataggg gcggcggaag acggccagaa
     3001 ccttgtcgcc gagcgcgcgc gcatcgaacc gcgcgccgga acggcggcgc ggcgcgggcg
     3061 atgcggacgg atttttcggt ttggcgaaca tgcggcgcgg accagggatt cggagagcgc
     3121 cagtctgccc gaaacagggt taacaaaggc taacgaaagc tgaacgcgcc gtgaaattcg
     3181 ccttaacccg gcgcgctttc cggccatgtg tcaggctcgt ggcccgtgcg caattccctg
     3241 gaacggaaaa ggcctcccgt cgccgggagg cccgatttta tttcgatgaa tcggaggcgc
     3301 ctgacgaaga cgccgcgccg ccggaggatg acgatgtggc ggacgacgcc gaggccgcgc
     3361 tgctgctgct ggcgccgtcc gcgcccggct tggtcagctt ggcgaagcgg ctgcccggct
     3421 tgtcggccag gtcggcggtg agcaggacca cgcccttcgg atgcgccctg acctcggcga
     3481 catt
//
LOCUS       DASVLL010000356         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_130760,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000356 DASVLL010000000
VERSION     DASVLL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130760"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1061)
                     /locus_tag="VF402_05960"
     CDS             complement(120..1061)
                     /locus_tag="VF402_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7799873.1"
                     /translation="MSNILHAFRASFHKSGVCNRPMAALAVVIAIVAAVASGHAKAAT
                     PFPNAAHARFSVAVSGDGPDVILIPGLASSAAVWDGTVDHLKGRYRLHVLNLAGFADE
                     PAGANAQGDVLAPSVAALDAYIKANRLERPVIVGHSLGGLMALMLAEAHPEDAGRLII
                     ADSLPFAGLMMGPQATAAAIAPQAKAMRDGVIALPADAFRTQQQATMARMVTADADRD
                     RIVQWSLDSDRRVLAQAFYEDLTEDARPGLPALTAPVAVIYPVDAAQTPEATAAFYKA
                     AWAGAPDIAFTPIAASRHFEMLDQPAAFYGALDAALK"
     gene            complement(1071..1247)
                     /locus_tag="VF402_05965"
     CDS             complement(1071..1247)
                     /locus_tag="VF402_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799874.1"
                     /translation="MPMRILAERKRSSLARLQPASWVIAAVMTALIAADPGHRLPPAL
                     FALFARLSGLILSH"
     gene            complement(1272..1478)
                     /locus_tag="VF402_05970"
     CDS             complement(1272..1478)
                     /locus_tag="VF402_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7799875.1"
                     /translation="MKQNLRELRTEMGLTQADLAARLGVSRQAVIALETDKHSPSLDL
                     AYKIAGVFDRPVEDIFANPYRVKA"
     gene            complement(1475..1870)
                     /locus_tag="VF402_05975"
     CDS             complement(1475..1870)
                     /locus_tag="VF402_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799876.1"
                     /translation="MNLNLKSPNARYTLGMGVSMALYVAALYASILSIQTRHPTGALL
                     YALAVLPALPIGGSIWVFLTYMEAVDEYVRGVLIRRFVIATGLSLFICTAWGFMEENA
                     GAHHFSLYLVYPLFWCLFGVVSAFLRKAS"
     gene            2017..>2198
                     /locus_tag="VF402_05980"
     CDS             2017..>2198
                     /locus_tag="VF402_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799877.1"
                     /translation="MWDVFAKREAKGMTTVVDFNAAKAQASARPPKTRFEALDILRGL
                     FMIGMLLANNAGDWKH"
BASE COUNT          386 a          660 c          798 g          354 t
ORIGIN      
        1 gtcgtttcgg gcggcatggc gcttcggatt cgttcccgtg gggatgcgcg attgtgccgc
       61 cgcaggtctt aatgacaccg taaagccgga ggttgtggtc aacctccggc gcacgcggtt
      121 catttcagcg ccgcgtcgag cgcgccgtaa aaggcggcgg gctggtccag catctcgaaa
      181 tggcggctgg cggcgatcgg cgtgaaggcg atgtccggcg cgcccgccca ggcggccttg
      241 tagaaggcgg cggtggcctc gggcgtctgg gccgcgtcca ccggatagat gacggcgacg
      301 ggcgcggtca gcgcgggcag gccgggacgc gcgtcctcgg tcaggtcttc gtagaaggcc
      361 tgcgccagca cgcgacggtc ggaatccagc gaccactgca cgatccggtc gcggtcggcg
      421 tccgccgtga ccatgcgcgc catagtcgcc tgctgctgcg tcctgaaggc gtcggcgggc
      481 agggcgatca cgccgtcgcg catcgccttc gcctgcggcg cgatggcggc ggcggtggcc
      541 tgcggcccca tcatcagccc ggcgaagggc agcgagtcgg cgatgatcag cctgcccgcg
      601 tcctccgggt gcgcctcggc cagcatcagc gccatcaggc cgccgaggct gtggccgacg
      661 atgaccggac gctcaagccg gttggccttg atataggcgt cgagcgccgc gaccgacggc
      721 gccagcacgt cgccctgcgc gttggcgccc gccggttcgt ccgcgaaccc ggccaggttc
      781 agcacgtgca ggcggtagcg gcccttcagg tggtcgacgg ttccgtccca gacggcggcg
      841 gacgaggcca ggccggggat caggatcacg tccggcccgt cgccgctgac cgcgacggaa
      901 aagcgcgcgt gggcggcgtt ggggaagggc gtcgcggctt tggcgtggcc tgaggccacg
      961 gcggcgacga tggcgatgac gacggccagc gcggccatcg ggcggttgca gacgccgctc
     1021 ttgtggaagc tggcgcggaa ggcgtggaga atgttggaca tgatatgacc tcagtgtgac
     1081 aggatgaggc ccgacaggcg ggcgaacagg gcgaacagcg ccggcggcag gcggtgtccg
     1141 ggatcggcgg cgatcagcgc ggtcatgacc gcggcgataa cccaactggc cggttgcagc
     1201 cgggcaaggg aggagcgctt acgctccgca aggatgcgca tgggcatgga gaagctcggt
     1261 atcgtaaggg gttaggcttt tacgcggtac gggttggcga agatgtcctc caccggccgg
     1321 tcgaagactc cggcgatctt gtaggctagg tccagcgacg gcgaatgctt gtccgtctcc
     1381 agcgcgatca ccgcctgccg ggacacgccg agccgcgccg cgaggtcggc ctgggtcagg
     1441 cccatctcgg tcctgagttc gcgcaggttc tgtttcatga cgccttcctc agaaaggcgc
     1501 tgacgacgcc gaacaggcac cagaacaggg gatagaccag atagagcgaa aaatggtgcg
     1561 cgcccgcgtt ctcctccatg aagccccacg ccgtgcagat gaacaggctc aggcccgtgg
     1621 cgatcacgaa gcgccggatc agcacgccgc gcacatattc gtcgaccgcc tccatatagg
     1681 tgagaaagac ccagatcgag ccgccgatcg gcagggcggg cagcacggcc agcgcgtaga
     1741 gcagggcgcc cgtcggatgg cgcgtctgga tgctcaggat cgaggcgtac agggcggcga
     1801 cgtacagcgc catcgagacg cccatgccga gggtgtagcg ggcgttgggc gatttcaggt
     1861 tcaggttcat ggaacgatct ccggtgtgta aggctgacct tacatgcacg aaaatgtcat
     1921 gtcaacctga catgcgcgaa aaatgtaagg ttgtcatgac atttttattg gttattcatt
     1981 ggtaattacc aatttggact tggcagaagc ccgcgcatgt gggatgtgtt cgcaaaacgg
     2041 gaggccaagg gcatgacgac ggtggtggat ttcaacgcgg cgaaggcgca ggcgagcgcc
     2101 agaccgccaa agacgcgctt cgaggcgctc gacattctgc gcggcctgtt catgatcggc
     2161 atgttgctgg ccaacaatgc gggcgactgg aagcatat
//
LOCUS       DASVLL010000357         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_22600, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000357 DASVLL010000000
VERSION     DASVLL010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22600"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF402_05985"
     CDS             complement(<1..314)
                     /locus_tag="VF402_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX7799878.1"
                     /translation="MIAALIAAAALNAASLTMPDMPLHDPFILADKASRTYYLYTSNN
                     PRLSGVCGLGTMAYESHDLEHWAAPKAVFTLPKGTWADGGAWAPEAHEWRGRYYLFTT
                     FH"
     gene            complement(311..>1528)
                     /locus_tag="VF402_05990"
     CDS             complement(311..>1528)
                     /locus_tag="VF402_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX7799879.1"
                     /translation="KHMYAGEGARWHEQHFGPQDKVGYKDLIPLFTAKNWSPEAWARL
                     FKAAGAKYVIPTAEHHDNFALWDSRLTPFNAVDMGPHRDLIGDLEKAVHAEGLKFGVS
                     NHGVENFTFINPDPAIDAKLKAEKADLYDPKWADFYHVADRSNAAMAAFLSDWVNRNL
                     ELIDDYHPDILWWDNGVNLRVLDPLKRYVAAYFYNRAAATGQQATLSTKYIAMAPSND
                     DTQQIGSVIDFEKVGKRSPAAIRLGGWQVDDTIGRQSWGYVEGLSVRPASEIVPLLID
                     TVAKGGNYTLNISPRADGVIPEDQQRTLLGIGKWLSRNGEAIYATHPWTQFGEGNWRF
                     TAKDGAIYAIALKWPGDTATIAALGEAAGKVASVALIGGGKVAFTQGPDGLHLKLPAQ
                     PDEIAYAFRVRMK"
BASE COUNT          243 a          500 c          480 g          305 t
ORIGIN      
        1 ttatggaagg tggtgaacag gtaatatctg ccccgccatt catgcgcttc cggcgcccag
       61 gccccgccat cggcccacgt ccctttcggc agggtgaaca ccgccttcgg cgccgcccag
      121 tgttcgaggt catggctctc atacgccatc gtccccagcc cgcagacgcc gctcaggcgc
      181 ggattgttcg aggtgtagag gtaataggtc cgcgacgcct tgtccgccag aatgaacgga
      241 tcgtgcagcg gcatgtccgg catggtcaga ctggcggcgt tcagggcggc ggcggcgatc
      301 agggcggcga tcatttcatc ctcacacgga acgcataggc gatctcgtcg ggctgggcgg
      361 gcagtttcag atgcaggccg tccggccctt gcgtgaaggc gaccttgccg ccgccgatca
      421 acgcgaccga cgcgaccttg cccgccgcct cgcccagcgc ggcgatggtc gccgtatcgc
      481 ccggccattt gagcgcgatg gcgtagatcg cgccgtcctt cgccgtgaat ctccaattcc
      541 cttcgccgaa ctgcgtccac ggatgcgtcg cgtagatcgc ctcgccgttg cggctcagcc
      601 acttgccgat gcccagcagg gtccgttgct ggtcctccgg gatcacgccg tcggcgcgcg
      661 gcgagatgtt gagcgtgtaa ttgccgccct tggccaccgt gtcgatcagc agcggcacga
      721 tctccgacgc cggacgcacg ctcaggccct cgacatagcc ccaggattga cgcccgatgg
      781 tgtcgtccac ctgccagccg ccgaggcgga tggcggcagg cgagcgcttg ccgaccttct
      841 cgaagtcgat cacgctgccg atctgctgcg tgtcgtcgtt cgacggcgcc atggcgatat
      901 atttggtcga cagcgtcgcc tgctggcccg tcgcggcggc gcggttgtag aagtacgccg
      961 ccacatagcg cttgagcgga tcgaggacgc gcaggttgac gccgttgtcc caccacagaa
     1021 tatccgggtg atagtcgtcg atcagctcca gattgcggtt gacccaatcg ctcaggaacg
     1081 ccgccatcgc cgcgttggag cggtccgcga catgatagaa atccgcccat ttcggatcgt
     1141 agaggtcggc cttttcggct ttcagcttcg cgtcgatagc cgggtccggg ttgataaagg
     1201 tgaagttctc gacgccgtga ttggagacgc cgaatttcag cccctcggca tggaccgcct
     1261 tttccagatc gccgatcagg tcgcgatgcg gccccatatc gaccgcgttg aacggcgtga
     1321 gccttgagtc ccacagcgcg aaattgtcgt gatgctcggc ggtcggtatg acgtatttcg
     1381 cgcccgccgc tttgaacagc ctggcccacg cttccgggct ccagtttttg gcggtgaaca
     1441 gcgggataag gtctttgtag cctaccttgt cctgcgggcc gaaatgctgt tcatgccagc
     1501 gcgcgccctc gcccgcatac atgtgctt
//
LOCUS       DASVLL010000358         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_344202,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000358 DASVLL010000000
VERSION     DASVLL010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344202"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VF402_05995"
     CDS             complement(<1..590)
                     /locus_tag="VF402_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX7799880.1"
                     /translation="MADANGAFVGQAQNADKSSEILLYGRANRHGVVAGATGTGKTVT
                     LQVLAQAFSDAGVPVFAADVKGDLSGIAMPGQPNDKLLARAQGMGLDLQPAAPPVVFW
                     DLFGQKGHPIRATISEMGPLLLARMLDLNDVQEGVLNIAFAVADKEGLLLLDLADLRA
                     MLKYLSDNAKEIGATYGQVSPTTIATIQRNLLALEN"
     gene            complement(627..>1547)
                     /locus_tag="VF402_06000"
     CDS             complement(627..>1547)
                     /locus_tag="VF402_06000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7799881.1"
                     /translation="GLRGFFVARFARLYPLYFVTFIAVALIQWAFHGLYGHDYIYGGN
                     TFVNFALNLVMAHQWLPHAVQNFNGPSWSISVEVFLYAVFFLLCRFRLQSVWTVAALS
                     VAGLFLRGLNPGADFMRGLPSFFLGGLVWYFVTELRRHDVLRRRLAVALAAVLPVLWG
                     LTYVWGYDSLWLRITQALSRHGVHWPPVTTVGAFCYVLTPLTLLFFGLQRGLWLKPER
                     LKRFEWVGDISYSVYLIHFPLQISLMLVLAQLPFAARARIFSSPFAYLAFMAAAMGLA
                     WFSYRYFETPMRKHIREWLAPRLARLRPVR"
BASE COUNT          321 a          505 c          499 g          222 t
ORIGIN      
        1 tggttctcca gcgccagcag gttgcgctga atcgtggcga tggtggtcgg cgagacctgg
       61 ccgtaggtcg cgccgatctc cttggcgttg tcgcttaagt atttcagcat ggcgcgcagg
      121 tcggccagat cgagcagcag cagcccctcc ttgtccgcca cggcgaaggc gatgttcagc
      181 acgccttcct gcacgtcgtt gaggtccagc atccgcgcca gcagcagcgg ccccatctcg
      241 gaaatggtgg cgcggatcgg atgccccttc tggccgaaca gatcccagaa caccaccggc
      301 ggcgcggcgg gttgcaggtc cagccccatg ccctgcgcgc gcgccagcag cttgtcgttc
      361 ggctggcccg gcatagcgat gcccgacagg tcgcccttca cgtcggcggc gaacacaggc
      421 acgcccgcgt cggagaaggc ctgcgccagc acctgcaagg tcaccgtctt gccggtgccg
      481 gtcgcgcccg cgacgacgcc gtgccggttg gcgcgcccgt acagcaggat ttcggacgat
      541 ttatcggcgt tttgcgcctg ccctacgaag gcgccgttgg cgtcggccat ctgatagtct
      601 cctgatgaag cgaactgacg gaatccttac cgcaccggcc tgaggcgcgc cagtctgggc
      661 gccagccatt cacgaatgtg tttacgcatc ggcgtctcga aataacggta gctgaaccag
      721 gccagcccca tcgccgccgc catgaaggcg agataggcga agggcgagga aaaaatccgc
      781 gcgcgcgccg cgaagggcag ttgcgccagc accagcatca gactgatctg caacggaaaa
      841 tgaatcaggt acaccgaata gctgatgtcg ccgacccatt cgaagcgttt cagccgttcc
      901 ggcttcagcc acaggccgcg ttgcaggccg aaaaacagca gggtcagcgg ggtcagcaca
      961 tagcagaagg cgccgaccgt ggtgacgggc ggccaatgca cgccgtgacg gctgagcgcc
     1021 tgcgttatgc gcagccacag cgaatcgtac ccccacacat aggtcagccc ccacagcacc
     1081 ggcaggaccg ccgccagagc gaccgccaga cgccggcgca aaacgtcgtg gcggcgcagc
     1141 tccgtcacga aataccagac gaggccgccc aggaaaaagc tcggcaggcc gcgcatgaaa
     1201 tcggcgccag ggttcagccc gcgcaggaac aggcccgcga cgctcaacgc cgcgaccgtc
     1261 cagacggatt gcaggcgaaa gcggcacagc aggaagaaca cggcgtacag gaacacctcg
     1321 accgagatcg accagcttgg gccgttgaaa ttctgcaccg cgtgcggcag ccattgatgc
     1381 gccatgacga ggttgagcgc gaaattgacg aaggtgttgc ccccgtagat gtagtcgtgc
     1441 ccgtacaggc cgtggaacgc ccactggatc agggcgacgg cgatgaaggt gacgaaatag
     1501 agcggataca gccgggcgaa acgcgcgacg aaaaagccgc gcagccc
//
LOCUS       DASVLL010000359         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_507453,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000359 DASVLL010000000
VERSION     DASVLL010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507453"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..583
                     /locus_tag="VF402_06005"
     CDS             68..583
                     /locus_tag="VF402_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799882.1"
                     /translation="MTVDRDLIAKEAAMGMYDDAPRRDDRRGSALLIAFPLLLIPVAI
                     YNLLAIIAMLGGSDGGSAYNATTQVLFNVPMPSPGTQWPVSIGDIILFGALICLFFEL
                     LKSTKSDRVAIMNHSLSMILFIVCLVEFLLLKPFATSTFFLVTSMTLMDVLAGFIVTA
                     ISARKDIDFGH"
     gene            complement(761..1021)
                     /locus_tag="VF402_06010"
     CDS             complement(761..1021)
                     /locus_tag="VF402_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799883.1"
                     /translation="MSHESPHLPVPAGPRVDHDAPRARSRLRGAPTDGAYAAQLLSGG
                     ERRGLKGGPDMLERAKTSYLQSEYSGQNDRRPRPGRVTRTEI"
     gene            complement(1094..1729)
                     /gene="phaR"
                     /locus_tag="VF402_06015"
     CDS             complement(1094..1729)
                     /gene="phaR"
                     /locus_tag="VF402_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX7799884.1"
                     /translation="MSETKTRAKKGDGERVIIKKYANRRLYNTATSSYVTLDNLSDMV
                     REGVDFVVFDAKTGDDITRSVLAQIIFEEESRGQNLLPIQFLRQLISFYGDSMQNLLP
                     TYLEMSLDGFAKQQERFRSQFQQAFGGAPGLGFFDEQVAQNLAMFDRAMRMFSPFSFA
                     NPGANPGAASAAAPTPDYAEPAPARDSDEVRELREQMAAMKAQIDKLAQKG"
BASE COUNT          384 a          648 c          695 g          441 t
ORIGIN      
        1 tcagcgccgt ccggggccgg tcagcgaaat ctgaccccgg cgcgtccggc gaaaggggga
       61 tgaggccatg accgtcgatc gcgatctgat cgccaaggaa gccgcgatgg gcatgtacga
      121 cgacgcgccg cgccgcgacg acagaagggg gtccgctttg cttatcgctt ttccgctgct
      181 gctgattccg gtggcgatct ataatctgct ggcgatcatc gccatgctgg gcggttcgga
      241 cggcggttcg gcctataacg ccacgacgca ggttttgttc aacgtgccga tgccgtcgcc
      301 gggcacgcaa tggccggtca gcatcggcga catcatcctg ttcggcgcgc tgatctgcct
      361 gtttttcgaa ctgctgaaat cgaccaagtc cgatcgcgtg gcgatcatga accattccct
      421 gtcgatgatc ctgttcatcg tgtgtctggt cgaattcctg ctgctgaagc cgtttgcgac
      481 ctcgaccttc tttctggtca cctcgatgac gctgatggac gtgctggccg ggtttatcgt
      541 cacggcgatt tcggcgcgca aggatatcga tttcgggcat taggggtggg gtttcaccta
      601 cccgaagacc gtaaaaatca acgccaccat cagcgtgtcg aaggccagcg tcatcaggcg
      661 caggggacgc gcgcggggtt tggcgaaggg gaacggcaca cgaagctcct gaggcgaaga
      721 acgccggaag ccatgcaagg atcgtgccgg tggttgagcc tcagatttcc gtgcgcgtga
      781 cgcggccggg gcgcgggcgg cggtcgttct ggccggaata ttccgattgc aggtaggagg
      841 tttttgcccg ctccagcatg tcgggaccgc ccttgaggcc gcggcgttcg ccgccgctca
      901 gcaactgcgc ggcataggcg ccgtcggtcg gcgcgccgcg aaggcgggat cgcgcgcgcg
      961 gcgcgtcatg gtccactctg ggaccggcgg ggacgggcag gtgcggggac tcatggctca
     1021 tgccgtccat tgtccccgca attggtgaag agtcaattgc cttaaccctt cgttaaccct
     1081 ctttaccggg gattcagccc ttttgcgcca gcttgtcgat ctgcgccttc atggccgcca
     1141 tctgctcgcg cagttcgcgc acctcgtcgc tgtcgcgcgc gggggcgggt tcggcgtagt
     1201 ccggcgtcgg cgcggcggcg gaagcggcgc ccggattcgc gcccgggttc gcaaatgaga
     1261 acggcgagaa catgcgcatg gcccggtcga acatggccag gttctgcgcc acctgctcgt
     1321 cgaagaagcc gaggccgggc gcgccgccga acgcctgctg gaactgcgag cggaagcgct
     1381 cctgctgctt ggcgaagccg tccagcgaca tttccagata ggtcggcaaa aggttctgca
     1441 tcgagtcgcc gtagaagctg atcaactggc gcaggaactg gatcggcagc aggttctggc
     1501 cgcggctctc ctcctcgaag atgatctggg ccagcacgga gcgggtgatg tcgtcgcccg
     1561 tcttggcgtc gaacacgacg aagtcgacgc cctcgcgcac catgtcggac aggttgtcca
     1621 gcgtgacgta ggagctggtc gcggtgttgt acagccgccg gttggcgtat ttcttgatga
     1681 tgacgcgctc gccgtcgccc ttcttggccc tggtcttggt ctcactcatg tcctgtcggt
     1741 tcccagatct tgaaagtcca gattgtataa ggcgcagccg cgttttcgcg ctgcaaccaa
     1801 catcgagact aactcctttt tcgcctatgc gtaaaggggc ggcgtgaacg gatgaaatag
     1861 accgataaat caattgatta gatgaatttg cgcctgcgat cacgagaaaa gtgatattgc
     1921 gcggcataag gcgcacaaaa cttcgccgcc aaacgcaggc gccggaacgg gtttgcacgt
     1981 gcgcgggcgc acaaaaagcg cacaatttgc gcggaacggc ccgcgcctat caaggttatg
     2041 tcatcgcctt gatttaaacc gtgaggtgtc ctacgccatg acgtagcaac cctgcttacc
     2101 cctaaggaag ctcgccttat gtccaacgaa gtcgtcatcg tttccgccgc ccgcacgccg
     2161 gtcggttc
//
LOCUS       DASVLL010000360         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_22853, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000360 DASVLL010000000
VERSION     DASVLL010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22853"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF402_06020"
     CDS             <1..275
                     /locus_tag="VF402_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX7799885.1"
                     /translation="RVIKLTGSRTDMADQHDSTDYVHGEMDIRQHESSYHLFNNLTKW
                     VSLCLAVLLVFLIVLTCTKAGFIAAFLSAAVLAVLGFFGLNRKPAH"
     gene            489..1625
                     /locus_tag="VF402_06025"
     CDS             489..1625
                     /locus_tag="VF402_06025"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX7799886.1"
                     /translation="MSLQLAIPAETSPGESRVAATPDTVKKWTAAGHAVRVQSGAGLG
                     AALPDDAFSAAGATVVKGANETIKGADIVLKVRGPSPDEIKALKPGAVVVATLDPYRE
                     RAALDALAKQQATAFAMEFVPRITRAQVMDVLSSQANLAGYRAVIEAAHAYGRAFPMM
                     MTAAGTVAPAKAFIMGVGVAGLQAIATARRLGAVVSATDVRPATKEQVESLGAKFLAV
                     EDEEFKNAQTAGGYAKEMSAEYKAKQAALTAAHIAKQDIVITTALIPGRQAPILVTKE
                     MVASMKPGSVLIDLAAPQGGNVEGCKADEIVDVNGVRIIGFSNIVASIAADASALYAK
                     NLQAFVGLLIDKDGQLAPDMEDEILKSALVAKDGAVVHPNLKKD"
     gene            1630..>1931
                     /locus_tag="VF402_06030"
     CDS             1630..>1931
                     /locus_tag="VF402_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEX7799887.1"
                     /translation="MEAVDPTIFRLAIFVLAIFVGYYVVWSVTPALHTPLMAVTNAIS
                     SVIIVGALIAAAASASGAGAGVWISKGAGGLASALAAVNIFGGFMVTRRMLAMYKK"
BASE COUNT          353 a          664 c          580 g          334 t
ORIGIN      
        1 ggcgcgtcat aaagcttaca ggttcgagga cagacatggc cgatcagcac gattccaccg
       61 actacgttca cggcgagatg gacatccgtc agcatgagtc ctcctatcac ctgttcaaca
      121 acctgaccaa atgggtcagc ctgtgcctgg ccgtcctgct ggtgttcctg atcgtgctga
      181 cctgcaccaa ggcgggcttc atcgccgcct tcctcagcgc cgccgtgctg gcggttctgg
      241 gctttttcgg cctgaacagg aagcccgcgc actgaagccg cccgcttgcg cgcgtcctgc
      301 gacgctgtgg cgttcagcgg tgataaagtc cgtccccgcg ttttcgtcgc gtaacaagtc
      361 tttgacttgc gcgacgtttg ctcataccaa gcaaaattat aaaaacaacg ggcgcaactt
      421 aacctttgcg tcaataataa agttgctttc gcaacattgt gccacagttt taagatgagg
      481 acgttatcgt gagcttgcag cttgccattc ccgccgaaac ctcgcccggc gaatcgcgcg
      541 tcgccgcgac gcccgacacc gtgaagaaat ggaccgccgc cggtcacgcc gtgcgcgtcc
      601 agtccggcgc gggcctcggc gccgccctgc ccgacgacgc cttcagcgcc gccggcgcca
      661 ccgttgtcaa aggcgcaaat gaaacgatca agggcgccga catcgtgctg aaggtgcgcg
      721 gcccgtcgcc ggacgagatc aaggcgctca aacccggcgc cgtcgtcgtc gccacgctcg
      781 acccgtaccg cgaacgcgcc gcgctcgacg cgctggccaa acagcaggcg acggccttcg
      841 cgatggagtt cgtgccgcgc atcacccgcg cgcaggtcat ggacgtgctg tcctcgcagg
      901 ccaacctggc cggttaccgc gcggtgatcg aggcggcgca cgcctatggc cgcgccttcc
      961 cgatgatgat gacggcggcg ggcacggtcg ccccggccaa ggccttcatc atgggcgtcg
     1021 gcgtcgcggg gctgcaagcc atcgccaccg cccgccgcct cggcgcggtc gtttccgcga
     1081 cggacgtgcg cccggccacc aaggagcagg tcgaatcgct cggcgccaag ttcctggcgg
     1141 tcgaggacga ggagttcaaa aacgcccaga cagccggcgg ctacgccaag gaaatgtccg
     1201 ccgaatacaa ggccaagcag gcggcgctga ccgcggcgca tatcgccaaa caggacatcg
     1261 tcatcaccac cgccctgatc cccggccgtc aggcgcccat cctcgtcacg aaagagatgg
     1321 tcgcctcgat gaagcccggc tcggtgctga tcgacctcgc cgcgccgcag ggcggcaatg
     1381 tcgagggctg caaggccgat gagatcgtcg acgtcaatgg cgtcaggatc atcggcttct
     1441 ccaacatcgt cgccagcatc gccgccgacg cctcggcgct ctacgccaag aacctgcaag
     1501 ccttcgtcgg cctgctgatc gacaaggacg gccaattggc gcccgacatg gaggacgaga
     1561 tcctcaaaag cgcgctggtc gctaaagacg gcgccgtcgt ccatccgaat ttgaaaaagg
     1621 attaagttca tggaagccgt cgatcctacg atcttccgtc tcgccatctt cgtgctggcg
     1681 atctttgtcg gctactacgt cgtgtggagc gtgacccccg ccctgcacac gccgctgatg
     1741 gccgtcacca acgccatttc ctcggtcatc atcgtcggcg cgctgatcgc cgccgccgcg
     1801 tccgcttcgg gcgcgggcgc cggagtgtgg atctccaagg gcgcgggcgg cctcgcctcg
     1861 gccctggccg ccgtcaacat cttcggcggc ttcatggtca cgcgccgcat gctggccatg
     1921 tacaagaaaa a
//
LOCUS       DASVLL010000361         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_397932,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000361 DASVLL010000000
VERSION     DASVLL010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397932"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..>1703)
                     /locus_tag="VF402_06035"
     CDS             complement(100..>1703)
                     /locus_tag="VF402_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HEX7799888.1"
                     /translation="VDVFEQTKVPSTAGAGLNVPPNGARICRWLGVDLDGGDPKGPDG
                     AIDGGRAAILESTRQFNADGSTTKRPFDHVTAAGDGAGFHHMHRLDLLMCLYKRVFEF
                     GPDSGAPCPITVHMDCRLAQLRQTADEVTATFSNGRTATGEVLVGADGINSATLRLAW
                     PNPRPKRWTEVTCFRGLIPRAAVASLRKADGSELDHNPIHSFSMDRHRTERSGATTYW
                     VRGGELLNVWIARYEPDSAAFEQEEGDWFPVSREEILREVDEAFTGSASRDDLLALAG
                     AIVRPTKWGLYDRDALETWVQGRICLLGDAAHPMLPTFGQGAAQSFEDAAALASAFAM
                     HGRDVATAMLHYERVRHYRATRFQLGSKFAFDHLRAKDTATQKALLEKLDERVSPAFA
                     HDKRGGEDDSWIYAYDARNIGPELPPKRLGPWDFRRAAKANYAAITRELWMPASPAKG
                     TRPVTREEVARHNTRDDCWIIISGKVYDITEWAPHHPGGAGIARMYAGKEATAEFGDF
                     HSAEAVAHMAHFCVGDLVEVPATSP"
BASE COUNT          269 a          566 c          571 g          297 t
ORIGIN      
        1 tccaaccgca ttcgcagcat cttcaagtcg aagggcgtgc tggcgatgga ttaggtcgcg
       61 caagcgatca gatcgattgc ccggcggtcg gcgtggccat cacggcgagg tggctggaac
      121 ctcgacgagg tcgccgacgc agaaatgagc catgtgcgcg accgcctcgg cgctgtggaa
      181 gtcaccgaat tccgcagtgg cttccttgcc ggcgtacatt cgggcgattc cggcgccacc
      241 ggggtgatga ggcgcccact cggtgatgtc gtaaaccttt cccgagatga tgatccagca
      301 gtcgtcgcgg gtgttgtgcc gggcgacctc ttcgcgcgtg accgggcgtg tgcccttggc
      361 gggggacgcc ggcatccaga gctcgcgggt gatcgcggcg tagttggcct tggcggcccg
      421 gcggaagtcc cacggtccta atctcttggg gggcaattcg ggaccgatat tgcgggcgtc
      481 gtacgcgtag atccaggagt cgtcctcgcc gccgcgcttg tcgtgggcaa aggccgggct
      541 gacccgttcg tcgagctttt cgagcagcgc tttctgcgtc gcggtgtcct tggcccgcaa
      601 atgatcgaag gcgaatttgg agccgagctg gaagcgggtg gcgcggtaat ggcgcacgcg
      661 ttcgtagtgc aacatcgcgg tcgccacatc gcggccgtgc atggcgaagg cgctcgcgag
      721 cgcggcggcg tcctcgaagg actgcgcggc gccttggccg aacgtcggaa gcatcgggtg
      781 cgccgcgtcg ccgagcagac agatgcggcc ctgcacccag gtttcgagcg cgtcacggtc
      841 gtagaggccc cacttcgtgg gacgaacgat cgcgcctgcc agagcgagca ggtcgtctct
      901 gctggcgctc cccgtgaagg cctcgtccac ctcgcgaagg atctcctccc ggcttacggg
      961 aaaccagtca ccctcctcct gctcgaacgc ggcagagtcg ggctcatagc gggcgatcca
     1021 cacgttcaac aattcgccgc cgcgcaccca gtacgtcgtc gccccgctcc tctcggtccg
     1081 atggcggtcc atgctgaagg agtggatggg gttgtggtcc aactcgctgc cgtcagcctt
     1141 gcggagcgag gcgaccgccg cccgcggaat gagcccgcga aagcaggtca cctcggtcca
     1201 gcgcttcgga cgcgggttcg gccacgccag ccgcaaggtg gcagaattga tcccgtcggc
     1261 gccgacgaga acctctcccg tagcggtgcg cccattcgaa aaggtcgccg tcacctcgtc
     1321 ggcggtctga cgcaactgcg ccagccggca gtccatgtgc acagtgatcg ggcacggcgc
     1381 cccgctgtcg ggaccgaact cgaacacccg cttgtacagg cacatcaaca ggtcgagcct
     1441 gtgcatgtga tgaaacccgg cgccgtcgcc cgcggcggtg acatgatcga acggcctctt
     1501 cgtcgtgctc ccatcggcgt tgaactgccg tgtcgactcc aggattgccg cccggccgcc
     1561 atcgatcgcc ccgtccgggc ccttgggatc cccgccgtcg aggtcgacgc ccagccaacg
     1621 gcatatccgc gccccgttgg gcgggacgtt caacccggcg cctgcggtgc tgggtacctt
     1681 cgtctgctca aaaacatcga ccg
//
LOCUS       DASVLL010000362         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_236905,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000362 DASVLL010000000
VERSION     DASVLL010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236905"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VF402_06040"
     CDS             complement(<1..1427)
                     /locus_tag="VF402_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7799889.1"
                     /translation="MAADKAQKTFGGLKTIVAASAAGTTFEWYDFFIFGALTATIAKN
                     FFASAGALGVILALATFAVGFIARPIGALVFGHIGDRKGRKGTFLYTILVMGLATVAI
                     GLLPTHAQIGDAAPLLLVALRLIQGFALGGEYGGAAIYVAEHAPANRRGTYTSWIQGS
                     ASFGLIAALIVILLTRRAMSPEAFEAWGWRIPFVVSAGLLVVSVYIRARTAESPAFKA
                     LHEAGQVAKAPMKEIFTRGRNLKGMLIALFGFMTAQGVIWYTTFFYTQVFLTAIVRID
                     PMTMNLLIMVMALISSGLYIGFAALSDRIGRKPVMIFGIVLASVSFFPGFQALMKYGN
                     PDLDAAVRRAPVVILADPAQCHFQFDLIGVKTFTSPCDVARNVVAGVNIPYVSENAPG
                     AAQAVIRVGGRTIPVGAAAGLDKAGVKAMKAGLSARLKSALGAAGYPDKADPKKSNHL
                     MMLVVLLLFAVAACALYGPMAALLV"
     gene            1562..1813
                     /locus_tag="VF402_06045"
     CDS             1562..1813
                     /locus_tag="VF402_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799890.1"
                     /translation="MKFSAPARAPEGHIAGLTSYARRARVRPVQIVVNIAEALLGLLG
                     VVVVILALRYGSFAGAGAALDSALACVGHAVAGFWPGNH"
     gene            1894..2511
                     /locus_tag="VF402_06050"
     CDS             1894..2511
                     /locus_tag="VF402_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX7799891.1"
                     /translation="MLEALIENNRRWSHRKTEIDPNFFRRLVAQQSPEYLWIGCSDSR
                     VPATEIVDLDPGEMFVHRNIANLAPAQDANSLSVLQFAVQVLKVKHILVVGHYGCGGV
                     RAALDSTGDSLIDHWLSPIRDVAHDHAHELDALEGSPKYDRLCELNVIQQVRNLSVNP
                     IVLRAWSEGQPLAIHGWVYALTNGYVTDLGVTVTSLEDRRRHFDI"
BASE COUNT          490 a          852 c          846 g          456 t
ORIGIN      
        1 tcgaccagca gcgcggccat cggcccgtac agcgcgcagg cggcgaccgc gaacagcagc
       61 agcacgacga gcatcatcag atggttgctc tttttcgggt cggccttgtc cgggtaaccg
      121 gcggcgccaa gcgcgctttt cagccgcgcg ctcaaaccgg ccttcatcgc cttgacgccc
      181 gccttgtcca gtcccgccgc cgcgccgacc gggatcgtcc ggccgccgac ccggatgacg
      241 gcctgggccg cgcccggcgc gttttcgctg acatagggga tattgacccc ggccacgaca
      301 ttgcgcgcca cgtcgcacgg cgaggtgaag gtcttgacgc cgatcaggtc gaactggaaa
      361 tggcactgcg ccggatcggc gagaatgacg accggcgcgc ggcgcacggc ggcgtcgaga
      421 tcgggattgc cgtacttcat cagcgcctga aaaccgggga agaagctgac cgaggccagc
      481 acgatgccga agatcatcac cggcttgcgc ccgatgcggt cggacagggc ggcgaagccg
      541 atatagaggc cgctggagat cagcgccatg accatgatca gcaggttcat ggtcatcggg
      601 tcgatgcgca cgatggcggt caggaacacc tgcgtgtaga agaaggtggt gtaccagatc
      661 acgccctgcg cggtcatgaa gccgaacagc gcgatcagca tgcctttcag attgcgtccg
      721 cgcgtgaaga tctccttcat cggcgccttg gcgacctgac cggcctcgtg cagcgccttg
      781 aaggccgggc tttcggcggt gcgggcgcgg atatagaccg agacgacgag caggcccgcc
      841 gagacgacga acggaatacg ccagccccac gcctcgaacg cctcgggact catggcgcgc
      901 cgggtcagca aaatgacgat cagcgccgcg atcaggccga aactggccga gccctgaatc
      961 cagctcgtat aggttccgcg ccggttggcg ggcgcgtgtt cggcgacata gatggccgcg
     1021 ccgccgtatt cgccgcccag agcgaagccc tgaatcagcc gcaaggccac cagcagcagc
     1081 ggcgcggcgt cgccgatctg cgcatgggtc ggcaacagcc cgatggcgac ggtggccagc
     1141 cccatgacga ggatggtgta gaggaaggtg cccttgcgcc ccttgcggtc gccgatatga
     1201 ccgaagacca gcgcgccgat cggccgggcg atgaagccga ccgcgaaggt ggccagcgcc
     1261 aggatcaccc ccagcgctcc ggcgctggcg aaaaagtttt tcgcgatggt cgcggtcaag
     1321 gcgccgaaga tgaagaagtc gtaccattcg aaggtggtgc ccgccgccga ggccgccacg
     1381 atggtcttca atccgccgaa ggttttttgc gccttatccg ccgccacgcc gccacccttt
     1441 tcctgtccgg ttatgatgat gcagccattt gaccaatatc cccgttattg gcaagtgcgc
     1501 cgccatctgg tccgatgcgg aatttgcggt taagctcatg cgtatcgagg gaggagacgc
     1561 catgaaattt tccgcacccg cgcgcgcgcc ggaaggccat atcgccggac tgacctctta
     1621 tgcgcgccgc gcgcgcgtgc gtccggtcca gatcgtcgtc aacatcgccg aggccctgct
     1681 gggcctgctg ggcgtggtgg tcgtcatcct ggccctgcgc tacggctcgt tcgccggggc
     1741 gggggcggcg ctcgattcgg cgctggcctg cgtcggccac gccgtcgcgg gcttttggcc
     1801 gggaaatcat taaccctgag ggcgaaaaag acttgatcca caggctgcgg cgtttatagc
     1861 tccccccaaa gtgagaggga gaccgggtcc gacatgttgg aagcgcttat cgagaacaat
     1921 cgccgttggt cgcatcgcaa gaccgagatc gatccgaact tcttccgccg cctcgtcgcc
     1981 cagcaatcgc cggaatatct gtggatcggc tgttcggatt cgcgcgtgcc ggccaccgaa
     2041 atcgtcgatc tcgatccggg cgagatgttc gtccaccgca atatcgccaa cctggccccg
     2101 gcgcaggacg ccaacagcct gagcgtgttg cagttcgccg tgcaggtgct gaaggtcaag
     2161 cacatcctcg tcgtcggcca ttacggctgc ggcggcgtgc gcgcggcgct ggattcgacg
     2221 ggcgacagcc tgatcgacca ctggctgtcg ccgatccgcg acgtggcgca cgatcacgcg
     2281 catgagctgg acgcgctgga gggcagcccc aaatacgacc gcctgtgcga gctgaacgtg
     2341 atccagcagg tgcgcaacct gtcggtcaat ccgatcgtgc tgcgcgcctg gagcgaaggc
     2401 cagccgttgg cgattcacgg ctgggtctat gcgctgacca acggctacgt caccgatctg
     2461 ggcgtcaccg tgacctcgct cgaagaccgg cgccggcact tcgacatctg agcgatttac
     2521 ctttcggtca tagcggttaa ttggcgttaa cccatcgaat tcattgccaa aaaataaccg
     2581 aacaacatcg gttttttatc acgcgtaaag accttcttca ggatttcagc cgcacattcc
     2641 agcc
//
LOCUS       DASVLL010000363         4035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_292220,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000363 DASVLL010000000
VERSION     DASVLL010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292220"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VF402_06055"
     CDS             complement(<1..847)
                     /locus_tag="VF402_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7799892.1"
                     /translation="MAFPVPYAAAPTRYDAMPYRRCGRSGLKLPAISLGLWQNFGGDD
                     VFETGRAILRCAFDSGVTHFDLANNYGPPYGSAEENFGRVMATDFRTHRDELVISTKA
                     GWDMWPGPYGDVGGSRKYLIASCDQSLKRMGLDYVDIFYSHRVDPETPLEETMGALAT
                     LHRQGKALYVGISSYSPELTRKAAEILKSEGVPLLIHQPSYSMLNRWIEEGLLDTLED
                     LGVGCIAFSPLAQGLLSRKYLNGVPDDARAAKGGSFRTGMLTEDNIRRIRELNAIAEG
                     RGQTLA"
     gene            complement(853..1272)
                     /locus_tag="VF402_06060"
     CDS             complement(853..1272)
                     /locus_tag="VF402_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX7799893.1"
                     /translation="MQTFRSLGESEARIAIDACIADLKRRDKAATVAVADSHGELIAL
                     WRMDGANLPSIVIAGNKAYTAARVRGDSGDMGRDVKSTGDDAHYHGDARYVGWDGGAV
                     VIHNGDVLGAVAVSGLIGEEDIAVARVGVDAILQVLG"
     gene            1440..3902
                     /locus_tag="VF402_06065"
     CDS             1440..3902
                     /locus_tag="VF402_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7799894.1"
                     /translation="MKRAHKQRMALSAALIALIAAPAVAGAPQVHPEKWPASHSPVGL
                     DKATEAKITAILKRMSLEEKVGQVIQPEIRYITPEEVAQYHIGTIENGGGSWPEQNKH
                     ATVADWVKLSETYYQAALRGNRQGVPLLWATDAVHGDNNVFGATLYPHNIGLGAMHDP
                     ALMEKIGAATAQEVRATGLDWAFAPTIAVARDDRWGRTYESYSEDPKIVSSYAAAMVK
                     GLEGEGATFLDRDHVIATAKHFLGDGSTDGGRDQGDSLASEDDLIRLHAQGYAAAVDA
                     GVQSVMASYNSWHGTKMHANKALLTDVLKGRMGFDGMIMGDWNAHGQIPGCDNGDCPI
                     TFNAGLDVFNAPTDWKALYGHMIAEVKAGTIPQSRLDDAVRRILRVKFRAHLFDEPSP
                     AHRPGAGGDIGTPEHRAIARQAVAESLVLLKNNGVLPLKPSARILIAGDGADNIAMQS
                     GGWTSSWQGADNANADFPGATSIYQGLKAQIEAAGGQALYSPDGVTPTKPDAAIVVFG
                     ETPYAEFMGDQPDVALHHDNADSLALLKTLKAEGVPVVAVLLSGRPLYVNPQINAADA
                     FVAAWLPGSEGEGVADVLLGRKDFTGRLAFSWPKRPDQTPLNVGDADYDPQFAYGYGL
                     SYAQPADTPQLPEVAETTKYGEKNVYFTKGPNGGNVWNGYKVSIGDSNAPHMDYVGTR
                     TTLYGTAALTVEPQDDGALHAVWNGKSDAWLEIGADKPSDIAREANGAMRLAVTLRVN
                     AAPSGAVKLGVGAASLLVTAPLKAAGTGYAQIAVPLSCFKTQDLSKTPTVLRLETAGT
                     LDVSISDVRLTEAQGDGACPAD"
BASE COUNT          720 a         1368 c         1316 g          631 t
ORIGIN      
        1 gcgccagggt ctggccgcgc ccttcggcga tggcgttcag ttcgcggatg cggcggatat
       61 tgtcctcggt cagcatgccg gtgcggaacg agccgccctt ggccgcgcgc gcgtcgtccg
      121 gcacgccgtt caggtatttg cggctcaaaa gcccctgcgc cagcggcgaa aaggcgatgc
      181 agccgacgcc gagatcttcc agcgtatcca gaagcccttc ctcgatccag cggttgagca
      241 tcgaatagga cggctggtgg atcagcagcg gcacgccctc ggatttgaga atctccgccg
      301 ccttgcgcgt caactccggc gaataggacg agatgccgac atagagcgcc ttgccctgac
      361 ggtgcagggt ggccagcgcg cccatcgtct cctccagcgg cgtttcggga tcgacgcggt
      421 gcgaatagaa gatgtcgaca tagtcgaggc ccatgcgctt caggctctgg tcgcaggacg
      481 cgatcaggta cttgcgcgag ccgccgacgt ctccgtaggg ccctggccac atgtcccagc
      541 ccgccttggt cgagatcacc agctcgtcac ggtgcgtgcg gaaatccgtc gccatcaccc
      601 ggccgaaatt ttcctccgcc gaaccgtagg gcgggccgta attgttggcg aggtcgaaat
      661 gggtgacgcc ggaatcgaag gcgcagcgca ggatggcgcg gcccgtctcg aacacgtcgt
      721 cgccgccgaa attctgccac aggccaagcg agatggcggg cagtttcagc cccgaccgac
      781 cgcaacggcg gtagggcatg gcgtcatagc gcgtcggcgc ggcggcatag ggaacgggaa
      841 aagccatatc gatcaaccca agacttgcaa aatcgcgtcg acgccgacgc gggcgacggc
      901 gatgtcctcc tcgccgatga ggccggaaac ggccacggcg ccgaggacgt cgccgttgtg
      961 aatgacgacc gcgccgccgt cccagccgac atagcgcgcg tcgccgtgat agtgggcgtc
     1021 gtcgccggtc gatttgacgt cgcggcccat atcgccggag tcgccgcgca cgcgggcggc
     1081 ggtataggcc ttgttgcccg cgatcacgat ggagggcagg ttggcgccgt ccatgcgcca
     1141 cagcgcgatc agttcgccgt ggctgtccgc gaccgcgacg gtggcggcct tatcccgccg
     1201 cttcaggtcg gcgatgcagg cgtctatggc gattcgcgcc tcgctttcgc cgaggctgcg
     1261 gaaggtttgc atggcgtgtc tcaacccgtt gttcttgtcc ttgatccccg acttaggcgg
     1321 cttggcgggc ggaagtcaag cggcctgatc tccttttgca accgtttaca aagcgcttga
     1381 cgacagcgct gtcatggttc ctgatgcgca aaacaaaaat ttacagcgag gaacccatta
     1441 tgaagcgcgc ccataaacag cgcatggcgc tgtccgccgc cctgatcgcc ctgatcgccg
     1501 cccccgccgt cgcgggcgcc cctcaggtcc acccggaaaa atggccggcc agccattcgc
     1561 ccgtcggtct ggacaaggcg acggaggcga agatcacggc catcctgaaa cgcatgagcc
     1621 ttgaggaaaa ggtcggtcag gtgatccagc cggaaatccg ctacatcacg ccggaggagg
     1681 tcgcgcaata ccatatcggc accatcgaga acggcggcgg ctcatggcct gagcagaaca
     1741 agcacgccac cgtcgccgac tgggtgaagc tgtccgaaac ctattatcag gccgcgctca
     1801 ggggcaaccg gcagggcgtg ccgctgttgt gggcgaccga cgccgtgcat ggcgacaaca
     1861 atgtcttcgg cgccaccctt tacccgcaca atatcggcct cggcgccatg cacgatccgg
     1921 cgctgatgga aaaaatcggc gccgcgacgg cgcaggaggt gcgcgccacc ggcctcgact
     1981 gggcgttcgc gccgaccatc gccgtggcgc gcgacgaccg ctggggccgg acctatgaga
     2041 gctattccga agacccgaag atcgtctcat cttacgccgc cgcgatggtc aaggggctgg
     2101 agggcgaagg cgcgaccttt ctcgaccggg accacgtcat cgctaccgcc aagcacttcc
     2161 tcggcgacgg ctcgacggac ggcgggcgcg atcagggcga cagcctggcc tccgaggacg
     2221 acctgatccg cctgcacgcg cagggctacg ccgccgccgt cgacgcgggc gtgcagtcgg
     2281 tcatggcctc gtacaattcc tggcatggga ccaagatgca cgccaataag gcgctgctga
     2341 ccgacgtgct gaaaggccgg atgggcttcg acggcatgat catgggcgac tggaacgcgc
     2401 acggccagat tccgggctgc gacaacggcg attgcccgat caccttcaat gcgggcctcg
     2461 acgtgttcaa cgccccgacc gactggaagg cgctctacgg ccacatgatc gcggaggtga
     2521 aggccgggac tatcccgcaa agccgcctcg acgacgccgt gcgccgcatc ctgcgcgtca
     2581 agttccgcgc ccatctgttc gacgagcctt cgcccgccca ccgtcccggc gcgggcggcg
     2641 atatcggcac gcccgaacac cgcgccatcg cgcgtcaggc cgtggcggaa tcgctggtgc
     2701 tgcttaaaaa caatggcgtc ctgccgctca aaccctcggc gcgcatcctg atcgcgggcg
     2761 acggcgccga caatatcgcc atgcagtcgg gcggctggac ctcaagctgg cagggcgcgg
     2821 acaacgccaa tgccgacttc cccggcgcga cctcgatcta tcagggcctg aaggcgcaga
     2881 tcgaggcggc gggcggtcaa gcgctctaca gccccgacgg cgtgaccccg accaaacccg
     2941 acgccgccat cgtcgtgttc ggcgaaaccc cttacgccga gttcatgggc gaccagccgg
     3001 acgtcgccct gcaccacgac aacgccgaca gcctcgccct gttgaagacg ctcaaggccg
     3061 aaggcgtgcc ggtcgtggcc gtgctgctgt cgggccgtcc gctctatgtc aacccccaga
     3121 tcaacgccgc cgacgccttc gtggcggcat ggctgcccgg ctccgagggc gagggcgtgg
     3181 ccgacgtgct gctgggccgg aaggacttta ccggcaggct cgccttctca tggccgaagc
     3241 gcccggacca gacgccgctc aatgtcgggg acgccgatta cgacccgcaa ttcgcctacg
     3301 gctatggcct ctcttacgcg cagcccgccg acacgccgca actgcccgaa gtcgcggaga
     3361 cgaccaaata cggcgagaag aacgtctatt tcaccaaggg cccaaacggg ggaaatgtct
     3421 ggaacggcta taaggtctcg atcggcgatt ccaacgcgcc gcacatggac tatgtcggca
     3481 cgcgcacgac gctgtacggc acggcggcgc tcaccgtcga gccgcaggat gacggcgccc
     3541 tgcatgcggt ctggaacggc aagtcggacg cctggctgga gatcggcgcc gataagccgt
     3601 ccgacatcgc gcgcgaggcc aacggcgcga tgcggctggc cgtcaccctg cgcgtcaacg
     3661 ccgccccgtc cggcgcggtc aagctcggcg tcggcgcggc tagcctgctg gtgaccgcgc
     3721 cgttgaaggc cgccggaacc ggctacgcgc agatcgccgt gccgctgtcg tgcttcaaga
     3781 cgcaggacct gtcgaaaacg cccaccgtgc tgcggctgga aaccgccggg acgctggacg
     3841 tgtcgatctc ggacgtgcgc ctgaccgaag cgcagggcga cggcgcctgc ccggcggatt
     3901 aagccttctc cgccgccttc acatattcgt cgtagtgctg gttcagcatg tcgcgatatt
     3961 cgtcgaccag ccgcgtgacg atggcctcgc ggtcgcggac cagccgctcc ggctcggtct
     4021 gcgtcaccat gaagg
//
LOCUS       DASVLL010000364         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_23017, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000364 DASVLL010000000
VERSION     DASVLL010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23017"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..825
                     /locus_tag="VF402_06070"
     CDS             139..825
                     /locus_tag="VF402_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7799895.1"
                     /translation="MLAIKPRARQIEFIGDSYTVGYGNTSPKPSCTRDEVWATTDTTQ
                     AFGPLTAKHYDADYQINAISGRGVVRNYGGFIADTLPQAYPYVLFDKQTKYDDPDWKP
                     QIIVVGLGTNDFTTPLHDGEKWKTRDDLHKDYEATYVKFVQELRANNPHAWFILMATD
                     QVDGEIQSEVNKVIAALQAAGESRIAFLPMNGLSFGGCDHHPTVADDKKVSDELIAFI
                     DQHPGLWQGQ"
     gene            complement(826..>2017)
                     /locus_tag="VF402_06075"
     CDS             complement(826..>2017)
                     /locus_tag="VF402_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7799896.1"
                     /translation="AIAEAREAADFCRYYARLARGQFGAPATLNGPVGERNTHSLHGR
                     GVFVCISPWNFPLAIFTGQIAAALAAGNAVLAKPAEQTPLIAHKAVKLFYGAGLHPDL
                     LALLPGDGAKIGAALVAHPGIAGVAFTGGTDTARAINRALAGRDGPIAPLIAETGGLN
                     GMFVDTTALKEQVIDDVIASAFGATGQRCSALRLLFLPRELADDYIAALKGAMDMLKL
                     GDPADFAVDVGPVIDLDALEALQRHIDTLTARGQVLHRVSAPELNGHYFAPTLAEIGD
                     PAELEREAFGPILHVVRYDSQAPEAMAEALAARGYGLTLGVHSRLESFAERIMRAVPA
                     GNVYVNRTITGAVVGVQPFGGEGLSGTGPKAGGPLALTRFAAERVVSVNITAQGGDPA
                     LLNL"
BASE COUNT          368 a          713 c          633 g          303 t
ORIGIN      
        1 acgcttaaca agccgggaga gatcatcaag acttacaaag gccttggcga aggccaacac
       61 atcattcgga ttgagcgagc aaccgaagat cagagtgcga caagcacttt tgaaggcttc
      121 tttgcgcctg cggggccaat gttggcgatt aagccgcgcg cgcgccagat cgagttcatc
      181 ggcgactcct acacggtcgg ctacggcaac acctcgccca agccgtcatg cacgcgcgac
      241 gaggtctggg ccacgaccga cacgacgcag gctttcgggc cgctgacggc gaagcattat
      301 gacgccgact atcagatcaa cgccatttcc gggcgcggcg tcgtgcgcaa ctatggcggc
      361 ttcatcgccg acaccctgcc gcaagcctat ccttacgtgc tgttcgacaa gcagacgaaa
      421 tacgacgacc cggactggaa gccgcagatc atcgtggtcg gcctcgggac caacgacttc
      481 accacgccgc tgcacgacgg tgaaaagtgg aagacccgcg acgacctgca caaggattac
      541 gaagccacct acgtcaaatt cgtgcaggaa ctgcgtgcga acaacccgca cgcctggttc
      601 atcctgatgg ccaccgatca ggtcgacggc gaaatccagt ccgaggtcaa caaggtcatc
      661 gccgccctgc aagccgcggg cgaaagccgc atcgccttcc tgcccatgaa cggcctgtcg
      721 ttcggcggct gcgaccacca cccgaccgtc gcggacgaca agaaggtgtc ggatgagttg
      781 atcgccttca tcgaccagca tccgggcctc tggcagggtc aatagtcaga ggttcagcag
      841 ggcgggatcg ccgccctgcg ccgtgatatt gacgctcacc acgcgctcgg cggcgaagcg
      901 cgtcagggcc agcggaccgc ccgccttcgg ccccgtgccg gacagcccct cgccgccgaa
      961 cggctgcacg ccgaccaccg cgccggtgat cgtgcggttg acatagacat tgcccgccgg
     1021 aaccgcgcgc atgatccgtt ccgcgaacga ttccagccgc gaatgtacgc ccagggtcag
     1081 gccgtagccg cgcgccgcca gcgcttcggc catcgcctcc ggggcctgcg aatcgtagcg
     1141 cacgacgtgc aggatcggcc cgaacgcctc gcgctccagt tccgccggat cgccgatctc
     1201 ggccagggtc ggcgcgaaat aatgtccgtt cagctccggc gcggatacgc gatgcagcac
     1261 ctgcccgcgc gccgtcaagg tatcgatatg cctttgcagc gcctccagcg cgtcgaggtc
     1321 gatcaccggc ccgacatcga cggcgaagtc cgccggatcg ccaagcttca gcatgtccat
     1381 cgcgcctttc agcgccgcga tatagtcgtc cgccagttcg cgcggcagga acagaagccg
     1441 cagcgccgaa cagcgctgcc ccgtcgcgcc gaaggccgag gcgatcacgt cgtcgatcac
     1501 ctgctccttc agcgcggtcg tatcgacgaa catgccgttc agcccgccgg tttccgcgat
     1561 cagtggcgcg atcggcccgt ccctgcccgc cagcgcgcgg ttgatcgcgc gcgccgtgtc
     1621 ggtcccgccg gtaaaggcca cgcccgcaat gcccggatgc gcgaccaggg ccgcgccgat
     1681 cttcgccccg tcgcccggca gcagggccag caggtcggga tgcagccccg cgccgtagaa
     1741 cagcttgacc gccttgtgcg cgatcagcgg cgtctgttcg gcgggcttgg ccagcacggc
     1801 gttgcccgcc gccagcgccg ccgcgatctg gccggtgaag atggccagcg ggaagttcca
     1861 cgggctgatg cagacgaaga cgccgcgccc gtgcaggctg tgcgtgttgc gctcgcccac
     1921 cggcccgttc agcgtcgccg gcgcgccgaa ctgcccgcgc gccagacggg cgtaataacg
     1981 gcagaagtcg gcggcttcgc gcgcctcggc gatggcg
//
LOCUS       DASVLL010000365         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_507946,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000365 DASVLL010000000
VERSION     DASVLL010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507946"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VF402_06080"
     CDS             <1..312
                     /locus_tag="VF402_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7799897.1"
                     /translation="SGGEQQMLAIARALMARPRLLLLDEPSLGLAPLIVRQIFDAIRA
                     LNAETGLTVFLVEQNAYHALKLAHRGYVMVNGLITLSGSGQELLERPEIRQAYLEGGR
                     H"
     gene            312..689
                     /locus_tag="VF402_06085"
     CDS             312..689
                     /locus_tag="VF402_06085"
                     /inference="COORDINATES: protein motif:HMM:NF045145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799898.1"
                     /translation="MEGLAHAFSIPHLLAEEDNFGVFFLVTVFLGGGAAWLTGRAAAQ
                     TWRPVWQVIIYSLILGGAVRFIHFSLFDGTLLSLHFYVVDAAVCLVIGILGFRVARAA
                     QMVRQYGWINEADGAMRWRRKPT"
     gene            771..1913
                     /locus_tag="VF402_06090"
     CDS             771..1913
                     /locus_tag="VF402_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7799899.1"
                     /translation="MMKTTTLGLALGFAIAAAGAANAQVKFGVQGPITGPNAAFGAQL
                     KNGAQQAVDDINAAGGINGQKLALQFGDDVSDPKQGVSVANKFSADGVKFVVGAFNSG
                     VTMPSSEVYQENGIVEVTPASTNPQITERKMWNIFRTCGRDDQQGAVAGAYILKQFKG
                     KKVAVVHDKTTYGKGLADETIKAMGKGGMKPVLYEGINVGEKDFSALVSKIKQSGADL
                     VYWGGLHTEGGLIVRQMRDQGVKAPLMGADGITTDEFAQVGGPGVEGTLMTFPPDPRN
                     NPAAKDAVAKFRAAKFEPEAYTLYSYAAVQVMADAAKQAKSNDPKKVAEKMHSGAHFK
                     TVIGDLSYDKKGDITRLDYVMYIWKKQADGKITYVECPGGDCSKAK"
BASE COUNT          411 a          697 c          687 g          361 t
ORIGIN      
        1 tccggcggcg agcagcagat gctggcgatc gcccgcgcgc tgatggcgcg gccgcgcctc
       61 ctcctgctcg acgagccgtc gctcggcctc gcccccctga tcgtgcggca gatcttcgac
      121 gcgatccgcg cgctcaacgc cgagaccggg ctgaccgtgt tcctggtcga gcagaacgcc
      181 taccacgcgc tcaagctggc gcatcgcggc tacgtcatgg tgaacggcct gataaccctg
      241 tccggcagcg gccaggaatt gctggagcgg ccggagatcc gccaggccta tctcgaggga
      301 ggccggcact gatggaaggc ctggcccacg cattctctat cccgcatctg ctcgccgaag
      361 aggacaattt cggcgtcttt tttctggtta ccgtgttcct gggcggcggt gcggcctggc
      421 tgaccggccg ggcggcggcg cagacctggc ggccggtatg gcaggtgata atctatagcc
      481 tgatcctcgg cggcgcggtc cggttcatcc atttcagcct gttcgacggc acgcttttgt
      541 ccttacattt ctatgtcgtt gacgccgccg tctgcctggt cattgggatc cttggattcc
      601 gcgttgcacg cgccgcgcag atggtccgcc agtatggctg gatcaacgag gcggacggag
      661 caatgcgctg gcgtcgcaaa ccgacctgag ttagcctttg gcttcctgta cttcactgtc
      721 ttcactgtct tcgctgtcat cgccggcacc accgttggag gggtagatag atgatgaaaa
      781 ccaccacgct cggcctcgcg ctgggctttg cgatcgccgc cgcgggcgcg gccaatgcgc
      841 aagtcaaatt cggcgttcag ggcccgatca ccggacccaa cgcagcgttc ggcgcgcagc
      901 tcaagaacgg cgcgcagcag gccgtcgacg acatcaacgc cgccggcggc atcaacgggc
      961 agaagctcgc cctccagttc ggcgacgacg tgtccgaccc gaagcagggc gtttcggttg
     1021 ccaacaaatt ctcggctgat ggcgtgaagt tcgtggtcgg cgccttcaat tccggcgtca
     1081 ccatgccctc ctccgaggtc tatcaggaga acggcatcgt cgaagtgacg ccggcctcga
     1141 ccaatccgca aatcaccgaa cgcaagatgt ggaacatctt ccgcacctgc ggtcgcgacg
     1201 accagcaggg ggcggttgcc ggcgcctaca tcctcaagca gttcaagggc aagaaggtcg
     1261 ccgtcgtcca cgacaaaacg acatacggca agggcctcgc cgacgagacc atcaaggcga
     1321 tgggcaaagg cggcatgaag ccggtgctgt atgaaggcat caacgtcggc gagaaggact
     1381 tctcggcgct ggtgtcgaag atcaagcagt cgggcgccga cctcgtctac tggggcggcc
     1441 tgcacaccga aggcggtctg atcgtccgtc agatgcgcga ccagggcgtc aaggcgccgc
     1501 tgatgggagc tgacggcatc accaccgacg agttcgcgca ggtcggcggt ccgggcgtcg
     1561 aaggcacgct gatgaccttc ccgccggatc cgcgcaacaa cccggccgcc aaggacgccg
     1621 tcgccaagtt ccgcgccgcc aagttcgagc cggaagccta cacgctgtac agctacgcgg
     1681 cggtgcaggt catggccgac gcggccaagc aggcgaaatc gaacgatccc aagaaggtgg
     1741 ccgagaagat gcactcgggc gcgcacttca agaccgtgat cggcgacctc tcctacgaca
     1801 agaagggcga catcacacgt ctcgactacg tgatgtacat ctggaagaag caggccgacg
     1861 gcaagatcac ctacgtcgag tgccccggcg gcgattgcag caaggccaag taagcctcgc
     1921 tccggcacga gaaagcgaaa agcccgccgt caccggcggg ctttttgttt ttgcgggcat
     1981 cggcgcgagc tagccgatct tccccagcgc cgggaaaacc tcgagcagcc agatgctgag
     2041 ctggctgatg ccgccggtca ggaacgcaat gccggtcagg accagaagcg cgcccatcgc
     2101 ctgctcgacg cggcgcaagt atttcttgaa gcgcgcgagg aacgcagcga aaggct
//
LOCUS       DASVLL010000366         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_23196, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000366 DASVLL010000000
VERSION     DASVLL010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23196"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF402_06095"
     CDS             <1..646
                     /locus_tag="VF402_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7799900.1"
                     /translation="ALYEPLSVLTTAARLSALDLSNVTAPVYVVPQAVMDTVAGFAIH
                     RGYLALGRARRALTLAEVVPAGRARLLVLYGVANTDNMGGLMRNAAAFGVDAAVLDET
                     CCDPLYRKAIRVSVGGVFSVPCVRVGNVIEALRALGVTPYALSPTGATRLDDVRPAER
                     SAFLFGAEGPGLPPDLMAACQSVRIDMHGGFDSLNVATTSGIVLHRMRAASGHG"
     gene            703..>1825
                     /locus_tag="VF402_06100"
     CDS             703..>1825
                     /locus_tag="VF402_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEX7799901.1"
                     /translation="MKVLMAAACLAAMTAGTAMAAETGYNPREAFAPRPMTQAVNRYR
                     SANGLPGPDYWQNRADYDIHAVLETKTPSLSGEEVITYTNNSPDTLDVLWLQLDQNLY
                     RKDSRGAHAAGDAQAGETSGGYTLDSVEIFDGKAFVAAQTLVDDTRLRVTLPRPLGGG
                     GAQVRVRIRWRFDIPGEWGGRMAWGKAKDGPIYDLAQWYPRMAVYDDIRGWDTLPYLA
                     QEFYLEYGDFDYAVNVPADMLVAGSGELMNAKEVLTPRELQRFERAKTSGATVMIRSP
                     DEIRDPATRPASKDGRLTWRFHMSHTRDVAFAASAVFAWDAAKIDLPGGKTAMAMSFY
                     PAESQGRDRWGRSTEYVKDAVENFSKRWYSYPWPAAINIA"
BASE COUNT          296 a          636 c          626 g          267 t
ORIGIN      
        1 cgccctctac gaaccgctca gcgtcctgac caccgccgcg cggctgagcg cgctcgacct
       61 ctccaacgtg acggcgcccg tctatgtcgt tccgcaggcc gtgatggaca cggtggcggg
      121 ctttgccatc catcgcggct atctggcgct ggggcgggcc agacgcgccc tgactctggc
      181 ggaggtcgtc cccgcgggcc gggcgcgcct gctggtgctg tacggcgtgg ccaacaccga
      241 caatatgggc gggctgatgc gcaacgcggc ggccttcggc gtcgacgcgg ccgtgctgga
      301 tgaaacctgc tgcgatcccc tgtaccgcaa ggcgatccgc gtctcggtcg gcggagtctt
      361 ctccgtgccg tgcgtgcgcg tcggcaatgt gatcgaggcc ctgcgcgcgc tcggcgtcac
      421 gccctatgcg ctcagcccca ccggcgcgac ccggctcgac gacgtgcggc ccgccgagcg
      481 ctcggccttc ctgttcggcg ccgaagggcc gggcctgccg cccgacctca tggccgcctg
      541 ccagagcgtg cgcatcgaca tgcacggcgg cttcgacagc ctgaacgtcg ccacgaccag
      601 cggcatcgtg ctgcaccgga tgcgcgccgc gtccggccac ggttgacagc cggtccgccg
      661 ggtgaaagca atgcggcggg caagcagacc gggcggcgga cgatgaaggt tttgatggcg
      721 gcggcctgtc tggccgcgat gacggcggga acggcgatgg cggcagagac aggctataat
      781 ccgcgcgagg cgttcgcgcc gcgtccgatg acgcaggccg tcaaccgcta ccgctcggcc
      841 aacggcctgc cggggccgga ctactggcag aaccgcgccg actacgacat ccacgccgtg
      901 ctggagacga aaacgccgtc gctgtcgggc gaggaggtca tcacctacac caacaacagc
      961 cccgatacgc tcgacgtcct gtggctgcaa ctggaccaga acctctaccg caaggacagc
     1021 cgcggcgccc acgccgccgg cgacgcgcag gccggcgaaa ccagcggcgg ctacacgctc
     1081 gattcggtcg aaatcttcga cggcaaggcg ttcgtggcgg cgcaaaccct cgtcgacgac
     1141 acgcgcctgc gcgtaaccct gccgcgaccg ctcggcggcg gcggcgcgca ggtccgggtg
     1201 cgcatccgct ggcgcttcga cattcccggc gaatggggcg ggcgcatggc gtggggcaag
     1261 gccaaggacg ggccgatcta cgacctggcg caatggtatc cgcgcatggc cgtctatgac
     1321 gatatccgcg gctgggacac gctgccctat ctggcgcagg aattctatct cgaatacggc
     1381 gacttcgact atgcggtgaa cgtccccgcc gacatgctgg tcgccggttc gggcgaactg
     1441 atgaacgcga aagaggtgct gacgccgcgg gagctgcaac gcttcgagcg ggcgaaaacc
     1501 tccggcgcca cggtgatgat ccgttcaccg gacgagatca gagatcccgc cacgcgcccg
     1561 gcctcaaagg acgggcgcct gacctggcgc ttccatatga gccatacgcg cgacgtcgcc
     1621 ttcgccgcct cggccgtgtt cgcgtgggac gcggcgaaaa tcgacctgcc cggcggcaag
     1681 accgcgatgg ccatgtcctt ctatccggcg gaaagccagg gccgcgaccg ctggggccgc
     1741 tcgacggaat atgtgaagga cgcggtcgag aacttctcga agcgctggta ttcctatccg
     1801 tggccggcgg cgatcaacat cgccg
//
LOCUS       DASVLL010000367         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_508171,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000367 DASVLL010000000
VERSION     DASVLL010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508171"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF402_06105"
     CDS             <1..320
                     /locus_tag="VF402_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7799902.1"
                     /translation="SQGRKRWRGDMDIEIACDMMEMAAHCDHLVLFSGDGDFRRLLES
                     VQRKGARVTVVSTVKTQPPMTSDELRRQADTFVDLADLQTVVGRPRSQNAPRPGQDED
                     DYA"
     gene            331..1110
                     /locus_tag="VF402_06110"
     CDS             331..1110
                     /locus_tag="VF402_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7799903.1"
                     /translation="MDLGIKGRYAIVCASSDGLGRACALELARAGCVVVINGRDGDKL
                     ARAADAIRGETGAEVIAVPGDVALAETQSRLIAAVPQVDILVNNNGGPPFRDFRDVTR
                     EDMLRGVTNNMIAPIEMVQRVIGGMTQRRFGRILNITSGSVKMPLGGLDLSSGARLGL
                     TGFLAGVARQVAHANVTINNLLPGAFDTRRLQSGQQASAEQRGINIDEVRAERAAAIP
                     ARRFGDPSEFAAACAFLCSAQAGYITGQSLLIDGGAFPGVM"
     gene            complement(1860..>2271)
                     /gene="smpB"
                     /locus_tag="VF402_06115"
     CDS             complement(1860..>2271)
                     /gene="smpB"
                     /locus_tag="VF402_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX7799904.1"
                     /translation="YFLENNLEAGIALTGTEVKSLRQGRANIAESYVSVEGDELCLIN
                     ADIPIYGHGNRFNHAPRRIRKLLLHRKEIHRFIVATQREGMTIVPTKLYFNDKGMVKL
                     EIALAKGKKIHDKRETEANRDWQRDKARLLRDRG"
BASE COUNT          408 a          799 c          647 g          417 t
ORIGIN      
        1 acagccaggg ccgcaagcgc tggcgcggcg acatggatat cgagatcgcc tgcgacatga
       61 tggagatggc cgcgcactgc gaccacctcg tgctgttctc cggcgacggc gatttccgcc
      121 gcctgctcga atcggtgcag cgcaagggcg cgcgcgtcac cgtcgtctcg accgtcaaga
      181 cccagccgcc gatgacctcg gacgagttgc gccgtcaggc cgacaccttc gtcgacctcg
      241 ccgacctgca aaccgtggtc ggccgcccgc gcagccagaa cgcgccccgg ccgggtcagg
      301 acgaagacga ctacgcctag gcggcaaatc atggatctcg gcatcaaggg ccgctacgcc
      361 atcgtctgcg cctccagcga cggacttggc cgcgcctgcg cgctggaact ggcgcgcgcg
      421 ggctgcgtcg tcgtgatcaa cggacgcgac ggcgataagc tggcgcgcgc cgccgacgcg
      481 atccgcggcg agaccggcgc ggaggtcatc gccgtccccg gcgacgtcgc gctggccgaa
      541 acgcaaagcc gcctgatcgc cgccgtcccg caggtcgaca tcctcgtcaa caacaacggc
      601 ggcccgccgt tccgcgactt ccgcgacgtc acgcgcgagg acatgctgcg cggcgtgacc
      661 aacaatatga tcgcgccgat cgagatggtt cagcgcgtga tcggcggcat gacgcagcgc
      721 cgcttcggcc gcatcctcaa catcacctcc ggctcggtga agatgccgct cggcggcctc
      781 gatctctcca gcggcgcgcg cctcggcctg accggcttcc tggcgggcgt ggcgcggcag
      841 gtcgcccacg ccaatgtcac catcaacaat ctgctgcccg gcgccttcga cacccgccgc
      901 ctgcaatcgg gccagcaggc cagcgccgaa cagcgcggaa tcaatattga cgaagtgcgc
      961 gccgaacgcg ccgccgccat cccggcccga cgcttcggcg acccgtccga attcgccgcc
     1021 gcctgcgcct tcctctgctc ggcgcaagcc ggctacatca ccggccaaag cctcctgatc
     1081 gacggcggcg cctttcccgg cgtgatgtag gacttgatcc acagatgaac acagatgttc
     1141 ataaggtcgc cacgctaccg ctaccgttcg accagcccac aattgcgggc gcaacgcgcc
     1201 tctcagaacc catccgcctc cgatagcggt agcgcgccgt taccctgaaa tcagtgctta
     1261 tctgtggatt caaaaccctg cctccgctcg cgcggacctt cttgtcaaag cctcccgatc
     1321 gacggcggcg cccttcccgg cgggacgtaa gtctttttat ccacagataa gcacaaattt
     1381 cataacaccg ccacgctacc gctatcgtcc ggtcagccca caattgcggg cgcaacgcgc
     1441 ctttcagaaa tccatccgct tccgatagcg gtagcgcgcc gttatcctga aatctgtgct
     1501 tatctgtgga ttcaaaaccc tgcctccgct cgcgcagacc ttcttgccaa agcctcgcga
     1561 tcgacggcgg cgcccttccc ggcgtggggt aagtcttttt atccacagat aagcacagat
     1621 gttcatggaa ccgccacgct accgctatcg tccggtcagc ccacaattgc aggcgcaacg
     1681 cgccctttca gacccaccca ctaccgatag cggtagcgtg aagccatcct gaaatctgtg
     1741 ctgatctgtg gataaaaacc ttgctcacgc tcgcatcgac cttatcggca aagcgtcatg
     1801 gccaacgtca tcgccttccc cagcgtgatg aaggccgtgt tgatctatgg ataaaaaccc
     1861 tacccccgat cgcgcagcag cctggccttg tccctctgcc agtcgcggtt ggcttcggtt
     1921 tcgcgcttgt cgtggatctt cttgcccttg gccagcgcga tctccagttt caccatgccc
     1981 ttgtcgttga aatagagttt cgtcggcacg atggtcatgc cttcgcgctg ggtggcgacg
     2041 atgaagcggt gaatctcttt ccggtgcagc agtaatttcc gtatccggcg cggcgcgtgg
     2101 ttgaagcggt tgccgtggcc gtagatcggg atatcggcgt tgatcaggca cagttcgtcg
     2161 ccttcgacgc tgacatagga ctcggcgatg ttcgcccgcc cctgccgcag cgacttcacc
     2221 tcggtcccgg tcagggcgat gcccgcctcc agattattct caaggaaata a
//
LOCUS       DASVLL010000368         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_508437,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000368 DASVLL010000000
VERSION     DASVLL010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508437"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VF402_06120"
     CDS             complement(<1..846)
                     /locus_tag="VF402_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799905.1"
                     /translation="MVAKYLAGMIAQGMTLMLCVFIACAAISPDAAQARTPSSRVFAS
                     QTPSSLYHDRMLEAYKLVSATSFQYTDDEIDQYGFRALHFRGDQRIYALWRVLYAYKD
                     DQNEEKFDAWHNRILAQAQADNDASLDVLARFMYQTYRNESNGFTELSEREWSAYLAM
                     SDPAIQNIVTLERIRQLQHFSQWADDIDMGDKLIVRLNAAGSDGAALLNVAHQTIIYA
                     LLAVGDKKAFAEHMLAIAMLLKDNSFFSQKMDMVYNMSLWAAKENDFDVAEKFQNLYS
                     YYANKY"
     gene            1045..1296
                     /locus_tag="VF402_06125"
     CDS             1045..1296
                     /locus_tag="VF402_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799906.1"
                     /translation="MLGDSLTGGDGGTGDSGEHGESQNDFFHGISLLRTRPKRSAIRP
                     FDEPGETADGFEAAAADREKNTGERLAAAVRARPSLTIY"
     gene            complement(1377..1784)
                     /locus_tag="VF402_06130"
     CDS             complement(1377..1784)
                     /locus_tag="VF402_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7799907.1"
                     /translation="MQSDLYPLPAVGVVCRRGGDVLLIRRGRAPRLGEWSIPGGKVGR
                     GESLRDAALRELYEETGVRARIGDLLAVYEIIEPDFHYVLIDYVADWIEGEPRAGDDA
                     DEARFLPFDAALLCVAHDDLRDVLRRAKDAAKA"
     gene            1878..3311
                     /locus_tag="VF402_06135"
     CDS             1878..3311
                     /locus_tag="VF402_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7799908.1"
                     /translation="MSTPALKTEIPAVAPEVLARIKAAVGEAGYSEDPMRLDPKLTEW
                     RGKWKGHTPLLVMPKSTEEVAAVVKICFEAGVAITPQGGNTGLVGGQIPFGEILISLE
                     RMKAVRDVAATDDTMVLETGLSLLEAQQIAERAGRYFPLSLAAEGTATVGGVISTNAG
                     GTAVLRYGVTRDLVSGLEVVLPNGEIFHGLKRLRKDNTGYDLKQLFIGAEGTLGIITA
                     ASLKLFPIMNSRATAIVAFESAQKAIDLLVRAKQETGGQVEAFELMGRYGLSLVLKHI
                     PDTREPLEAEYPWYALIEVASGDPDGAQAAMERVLGAAFEEELIVDGVIAQNETQAAE
                     FWRLREDHSAAEKAEGAAWKHDVSVPLSRMADYIAEGDKAIQAFLPGARLVAFGHVGD
                     GNVHFNVIVPEGMDAGEFNALRDAGAKVVHDLVHRYEGSISAEHGLGRMKTQEALLYK
                     DPVAVEAMRAVRLALDPKRIMNPHVLF"
BASE COUNT          730 a         1091 c         1041 g          624 t
ORIGIN      
        1 atatttattc gcataatagc tataaagatt ttggaatttc tccgcgacat cgaaatcgtt
       61 ttcctttgcc gcccagaggc tcatattgta aaccatatcc attttttgag aaaaaaacga
      121 attgtctttc aacaacatgg cgatagccag catatgctcg gcaaaagctt ttttgtcgcc
      181 caccgccagc aaggcgtaaa taatcgtctg atgcgcgacg ttcaataaag ccgcgccgtc
      241 actgccagcc gcgttcaggc ggacgatcag cttatccccc atatcgatat cgtcggccca
      301 ttgcgaaaaa tgctgcaact gacggatacg ctccagggta acgatattct gaatggccgg
      361 atccgacatc gccagatagg cgctccattc acgctcgctc agttccgtga agccgttgct
      421 ttcattgcga taggtctggt acatgaagcg cgccagcaca tcgaggctgg cgtcgttgtc
      481 cgcctgcgcc tgcgccagaa tccggttgtg ccaggcgtcg aatttttctt cgttctgatc
      541 gtccttgtag gcatagagca cgcgccacag cgcatagatg cgctgatctc ccctgaaatg
      601 gagcgcacgg aagccgtact gatcgatttc atcatcggta tattgaaatg acgtcgcaga
      661 gaccagctta tacgcttcca acatcctgtc atgatacaag gatgatggcg tctgggaggc
      721 aaatactcga gacgaagggg tccgagcctg cgcggcgtcg ggtgaaatcg ccgcacaggc
      781 gataaaaaca caaagcatga gcgtcatgcc ttgcgcaatc attcctgcca aatatttggc
      841 gaccacatta gtctccgaag atgctgctgt aacagcaaac cccaaagagc ttagaaatca
      901 tttaaaattt tggcgccgca acaaccaaaa aggtgggggg aagccttggc ttcaccccga
      961 ggcgacgcct catccttaca tggtgttgcc gaccggatcg acacaaaggc aaccataggt
     1021 cttcgagcag gtgcaggaag gaccttgctt ggcgacagcc tgaccggcgg cgacggcgga
     1081 accggcgaca gcggcgagca cggcgagagc caaaacgact tttttcatgg gatttccctt
     1141 ttacgaacac gaccaaagcg ctccgcgatc cgcccttttg acgaaccggg cgagaccgcc
     1201 gacggctttg aggccgccgc agcggaccgt gaaaagaaca ccggcgaacg attggccgca
     1261 gctgttcgcg cccgcccctc gttaacaatt tattaaccat atattttaag aaaaggcaac
     1321 ggaaacgcgc ccgattaatt cgaaaacaga ctatttttca gctcagaggc gtattttcag
     1381 gccttagcgg cgtctttggc gcgccgcagc acgtcgcgca gatcgtcgtg cgcgacacac
     1441 aaaagcgccg cgtcgaacgg caggaaacgc gcttcgtccg catcgtcgcc cgcgcgcggc
     1501 tcgccctcga tccagtcggc gacgtaatcg atcaggacgt agtgaaagtc cggttcgatg
     1561 atctcataaa cggccagcag atcgccgatt cgcgcccgca cgccggtctc ctcgtatagc
     1621 tcgcgcaggg ccgcgtcgcg caggctctcc cctcgcccga ccttgccgcc gggaatcgac
     1681 cattcgccca gccgcggcgc gcgcccgcgc cggatcagca gcacgtcgcc gccgcgccgg
     1741 cagaccacgc cgaccgccgg caggggatat aagtcggatt gcatcatggg ctcaggtttt
     1801 atgacttgcg caacgaagca tcctcgctta acgcccacgc agagtttccg caacgttcct
     1861 tatcgcgaga cgcccccatg tccacgcccg ctcttaaaac cgagattccc gccgtcgcgc
     1921 ccgaagttct ggcgcgcatc aaggccgccg tcggcgaggc cggctacagc gaagacccga
     1981 tgcggctcga tccgaagctg accgaatggc gcggcaagtg gaagggccat acgccgctgc
     2041 tggtcatgcc caaatcgacc gaagaggtcg cggcggtggt gaagatctgc ttcgaggccg
     2101 gggtcgcgat cacgccgcag ggcggcaaca ccgggctggt cggcggacag attccgttcg
     2161 gggaaatcct gatctcgctg gagcgcatga aggcggtgcg cgacgtggcg gcgaccgacg
     2221 acacgatggt tctggaaacc ggcctcagcc ttctggaggc gcagcagatc gccgagcggg
     2281 ccggacgcta tttcccgctg tcgctggccg ccgaaggcac ggcgacggtg ggcggcgtga
     2341 tttcgaccaa cgcgggcggc acggcggtgc tgcgctacgg cgtgacgcgc gatctggtgt
     2401 ccgggcttga ggtcgtgctg ccgaacggcg agatcttcca cggcctgaag cggctgcgca
     2461 aggacaatac cggctacgac ctcaagcagt tgttcatcgg cgccgaaggc actttgggga
     2521 tcatcacggc ggcgtcgctg aagctgtttc cgatcatgaa ttcgcgcgcc accgccatcg
     2581 tggcgttcga gagcgcgcaa aaggccatcg acctcctggt gcgcgccaag caggaaaccg
     2641 gcggccaggt cgaggcgttc gagctgatgg gccgctatgg cctgtcgctg gtgctcaagc
     2701 acattcccga cacgcgcgag ccgctggaag ccgaatatcc gtggtacgcg ctgatcgagg
     2761 tcgcttccgg cgatccggac ggcgcgcagg cggcgatgga gcgcgtgctg ggcgccgctt
     2821 ttgaggaaga actgatcgtc gacggcgtga tcgcccagaa cgagacgcag gccgccgaat
     2881 tctggcgcct gcgcgaagac cattccgccg ccgaaaaggc cgagggcgcg gcatggaagc
     2941 acgacgtctc ggtgccgctg tcgcgcatgg ccgactatat cgcggaaggc gacaaggcga
     3001 ttcaggcctt cctgcccggc gcgcggctgg tggcgttcgg ccatgtcggc gacggcaacg
     3061 tccacttcaa cgtcatcgtg cccgaaggca tggacgcggg cgaattcaac gccctgcgcg
     3121 acgcgggcgc caaggtcgtc cacgacctcg tgcatcgcta cgaaggctcg atttccgccg
     3181 aacacggcct cggccgcatg aagacgcagg aagccctgct ctacaaagac ccggtcgccg
     3241 tcgaagccat gcgcgccgtg cgtctggcgc tcgaccccaa gcgcatcatg aacccgcacg
     3301 tcctgttcta aaacccctct cccctccacg aggggagagg acgagagccg agcgtaagcg
     3361 aaggcgatcg ggtgaggggc cgcccgccga caaaacgcaa acgtcgcccc tcgcccgccc
     3421 tcatagcggc gccgacgcgc aacgcctaat cgaacagcgc gtcgatctcc gcctgcttgg
     3481 cgttct
//
LOCUS       DASVLL010000369         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_237532,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000369 DASVLL010000000
VERSION     DASVLL010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237532"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..299
                     /locus_tag="VF402_06140"
     CDS             111..299
                     /locus_tag="VF402_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799909.1"
                     /translation="MPDPVHEGQKVFSPDQLNQAGEDKEYFHPKKDHRYDVPKDANAH
                     ALKPDVEVAPGNKLRTQK"
     gene            321..692
                     /locus_tag="VF402_06145"
     CDS             321..692
                     /locus_tag="VF402_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7799910.1"
                     /translation="MKRFLPLLLLVSACSRPDAPLRAPDLANGRRQFAACAGCHSLSP
                     GVTVFGPSLYSVYGRKAGSLAGYDYSPAMRAYGKAWDEAALDAFLASPGRTVPGTRMG
                     FWGMRNAQDRRDLIAFLKNQP"
     gene            complement(689..1093)
                     /locus_tag="VF402_06150"
     CDS             complement(689..1093)
                     /locus_tag="VF402_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEX7799911.1"
                     /translation="MSLLNDFINAAGGGGGIAADLEQLTGHLGNGGLQAMLSQFEQGG
                     LGQVAQSWIGNGANLPVSTEQLQAVLGSEKVTALAQSLGIDTSQVAAALPGLVDHLTP
                     GGQLPLGNIEDMLGQAVNSGALGNLLGGLFKS"
     gene            complement(1155..1796)
                     /locus_tag="VF402_06155"
     CDS             complement(1155..1796)
                     /locus_tag="VF402_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX7799912.1"
                     /translation="MSTNALAPEHLAHLAAADPRLAPLCARFSHIGYRLRPGGFEALL
                     RLIVEQQLSVRAADTILSRVHAGIGEVTPQDLRDHPDDALRSYGLSRPKIAYAKALAE
                     AVLRGEFDAAALLVMDIETAAQTLLKLKGVGRWTAEVYLMFSEGRLDLFPVGDVALRE
                     AVGWLDALAARPDEIYCAERAALWSPYRSVAAHLLWAWYGAVRRGESTRALTA"
     gene            complement(1860..>2061)
                     /locus_tag="VF402_06160"
     CDS             complement(1860..>2061)
                     /locus_tag="VF402_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX7799913.1"
                     /translation="GVTLKESPRRDRIYTSTPQTVLAIANPPERTPDYESEATVTKRN
                     YGIVADTLRREYRVAQVGCIKS"
BASE COUNT          354 a          699 c          675 g          333 t
ORIGIN      
        1 tcgccgggga aaagtcaaag ggcggcttta cgccgcgttt gcgcgcatcc ggccgatccc
       61 aacccgcccc ttacgcgacc ggcgttatac tccggtcaag gaggcgcgcc atgcccgatc
      121 ccgttcatga aggccagaag gtgttttcgc cggaccagct caatcaggcc ggcgaggaca
      181 aggaatattt ccatccgaag aaggaccacc ggtacgacgt ccccaaggac gccaacgcgc
      241 acgcgctgaa gcccgatgtc gaggtcgcgc cgggcaataa gctcaggacg caaaaatagc
      301 ggcgtgctac agcgggcggc atgaaacgct ttctgccgct cctgctgctg gtgtccgcgt
      361 gcagccgccc ggatgcgccc ctgcgcgcgc cggaccttgc gaacggacgt cgccagttcg
      421 ccgcctgcgc cgggtgtcat tccttgtcgc ccggcgtgac cgtcttcggc ccgagcctct
      481 attccgtgta cggacgaaag gccggaagcc ttgccggtta tgactacagc ccggccatgc
      541 gcgcttacgg caaggcgtgg gacgaggccg cgctcgacgc gtttctggcc tcgcccggcc
      601 ggacggttcc ggggacgcgc atgggctttt ggggcatgcg gaacgcgcag gaccggcgcg
      661 acctgatcgc cttcctcaaa aatcagcctt agctcttgaa caggccgccg agcaggttgc
      721 ccagcgcacc ggaattgacc gcctgaccca gcatgtcctc gatattgccc agcggcaatt
      781 gcccgcccgg cgtcaggtga tcgaccagac ccggcagggc ggcggcgacc tgcgacgtgt
      841 cgatgcccag cgattgcgcc agcgccgtga ccttttccga gcccagcacg gcctgcaact
      901 gctcggtcga caccggcagg ttggcgccgt tgccgatcca ggattgcgcg acctggccca
      961 ggccgccctg ctcgaactgg ctcagcatgg cctgcaagcc gccgttgccg agatggcccg
     1021 tcagttgctc cagatcggcg gcgatcccgc ctccgccgcc ggcggcgttg ataaagtcgt
     1081 tcagcaggct catggctcgt tctcctcgga tacccagccc ggtaacggaa cgccgaaatc
     1141 cggcgggcgt caagtcatgc cgtaagcgct ctggtcgatt ctccgcgccg gaccgcgcca
     1201 taccacgccc agagcagatg cgcggcgacc gagcggtacg gcgaccacag cgccgcgcgt
     1261 tcggcgcaat agatttcgtc cggccgcgcc gccagcgcgt ccagccagcc caccgcctcg
     1321 cgcaaggcca cgtcgccgac cgggaacagg tcgagccgcc cttcgctgaa catcagatag
     1381 acctcggcgg tccagcgccc gacgcccttg agtttgagaa gcgtctgcgc cgccgtctcg
     1441 atatccatga ccagcagcgc ggcggcgtcg aactcgccgc gcagcacggc ctcggccagg
     1501 gccttggcgt aggcgatctt gggccgcgac aggccgtagg accgcaaggc gtcgtccggg
     1561 tggtcgcgca ggtcttgcgg cgtgacctcg ccgattccgg cgtggacgcg gctcaggatc
     1621 gtgtccgccg ccctgactga caattgctgc tcgacgatca gccgcagcag cgcctcgaac
     1681 ccgccgggcc ggagacgata gccgatatgc gaaaaccgcg cgcacaacgg cgcaaggcgc
     1741 ggatcggcgg cggccagatg cgcgagatgt tcgggagcga gggcgtttgt ggacattgaa
     1801 agacgttagc cacgaaacga agttcggcga agccaaactt cgtttcgtgg ctgaaaaaac
     1861 tacgacttga tacagcccac ctgcgccacg cgatattcgc ggcgtaaagt gtccgccacg
     1921 atgccgtaat tgcgtttggt gacggtggct tcggactcgt agtcgggcgt gcgctccggc
     1981 gggttggcga tggccagcac ggtttgcggc gtcgaggtgt agatgcggtc ccggcgcggg
     2041 gattccttca gggtcacgcc g
//
LOCUS       DASVLL010000370         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_23621, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000370 DASVLL010000000
VERSION     DASVLL010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23621"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VF402_06165"
     CDS             complement(<1..846)
                     /locus_tag="VF402_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7799914.1"
                     /translation="MTDLSKIRNFSIVAHIDHGKSTLSDRLIQFTGGLSAREMKEQVL
                     DNMEIERERGITIKAQTVRLNYKAKDGEDYVLNLMDTPGHVDFAYEVSRSLAACEGSI
                     LVVDASQGVEAQTLANVYQAIDNNHEIVPVLNKIDLPAAEPDRVRAQIEDVIGIDASD
                     AVLASAKSGLGIEDVLEAIVTRLPAPKGEENEPLKALLVDAWYDPYLGVVVLVRVFNG
                     RMKPGQQIKLMNAGASYRIDKLGVFQPKATDVDSLGPGEIGFFTAQIKEVSDAAVGDT
                     ITDERK"
     gene            1012..>2075
                     /locus_tag="VF402_06170"
     CDS             1012..>2075
                     /locus_tag="VF402_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HEX7799915.1"
                     /translation="MRHNKIARRAAWLSSCAWLLAGAAFAGPLHLNDKGYYETPGVNV
                     LVFSNWYDGLFADAKISGVELIHHGERTASNGDVRLSPTPGQWDPTGQFVDRKVDAAT
                     GTITATLKYPDYDFGYQIVAKPQGDGVQVSVVLDKPLPKALEGRAGFNLEFLPVVYFH
                     KSYLVDGKPGLFPRYPSSDMQIEPDARYAPQTGPDTKPLPIATGKSFVLAPEDAAERV
                     SVSSDSDISLYDGRNQAQNGWYVLRSMIPAGKTGAVVTWTVTPNAIPNWVRAPVIGHS
                     QLGYVPDQKKVAVIELDPNDKPAATAALLKINADGSETPVLTGEAKPWGHYLRYDYRT
                     FDFSSVTAPGLYEIAYEGQKT"
BASE COUNT          371 a          654 c          675 g          375 t
ORIGIN      
        1 cttgcgctcg tcggtgatgg tgtcgccgac ggcggcgtcg gagacttcct tgatctgggc
       61 ggtgaagaag ccgatctcgc cggggcctaa ggagtccaca tcggtcgcct tgggctggaa
      121 cacgcccagc ttgtcgatcc ggtacgaagc gcccgcgttc atcagcttga tctgctggcc
      181 gggcttcatc cgtccgttga acacgcgcac cagcacgacg acgccgaggt aaggatcgta
      241 ccacgcatcg accagcagcg ccttgagcgg ctcgttctcc tcgcccttcg gcgcgggcag
      301 gcgcgtgacg atggcctcca gcacgtcctc gatgccgagg cccgacttgg ccgaggccag
      361 cacggcgtcg gaggcgtcga tgccgatcac gtcctcgatc tgggcgcgca cccggtccgg
      421 ctcggcggcg ggcaggtcga tcttgttgag gaccggcacg atctcgtgat tgttgtcgat
      481 ggcctgatag acattggcca gggtctgcgc ctcaacccct tgcgaggcgt cgacgaccag
      541 gatcgaacct tcgcaggcgg ccagcgagcg cgacacctcg taggcgaagt cgacatggcc
      601 cggcgtgtcc atcaggttca gcacatagtc ttcgccgtcc ttcgccttat agttcaggcg
      661 cacggtctgg gccttgatgg tgatgccgcg ctcgcgctcg atctccatat tgtcgagcac
      721 ctgctccttc atctcgcgcg cgctcagacc gccggtgaac tgaatcagcc ggtcggaaag
      781 cgtcgatttg ccgtggtcga tatgggcgac gatggagaag ttgcggatct tggacaggtc
      841 ggtcatgcgc gcgcaactag cacggcgctc ggcaaagaga aagagggaag cgcgcggcgg
      901 tgtccggcgt gtaatcggtt acaaatcttt tgtgatcgcg gccttgcgac tggtcagacc
      961 gattggcgcg tgttagcgtt accaaaacaa cgacgacagg gactgttcac tatgcgtcac
     1021 aacaaaatcg cacgccgggc ggcgtggttg tcctcttgcg cctggctgtt ggccggggcg
     1081 gcgttcgccg ggcctttgca cctgaacgac aagggctatt acgagacgcc gggcgtcaat
     1141 gtgctggtgt tttccaactg gtatgacggg ttgttcgccg acgccaagat cagcggcgtc
     1201 gagctgatcc atcacggcga gcgcacggcg agcaacggcg acgtgcgcct gtcgccgacg
     1261 ccggggcaat gggatccgac ggggcagttc gtggaccgca aggtggacgc cgccacgggg
     1321 acgatcaccg cgactctgaa atatcccgac tacgatttcg gctatcagat cgtcgccaaa
     1381 ccgcagggcg acggcgtgca ggtcagcgtg gtgctggaca agccgctgcc gaaagcgctg
     1441 gagggccgcg ccgggttcaa tctcgaattc ctgcccgtcg tctatttcca caagagctat
     1501 ctcgtcgacg gcaagccggg cctgttcccg cgctatccgt cctccgatat gcagatcgag
     1561 ccggacgcgc gttacgcgcc gcagaccggc cccgacacca aaccgctgcc catcgccacc
     1621 ggcaagtcgt tcgttctggc gcccgaggac gccgccgagc gcgtatcggt gagcagcgac
     1681 agcgacatca gcctctatga cggccgcaat caggcgcaga acggctggta cgtgctgcgt
     1741 tcgatgattc ccgcgggcaa gaccggcgcg gtggtgacgt ggaccgtgac gccgaacgcc
     1801 attccgaact gggtgcgcgc gccggtgatc ggccattcgc agcttggcta tgtgcccgat
     1861 cagaagaagg tcgcggtcat cgaacttgac ccgaacgaca agcccgccgc gacggccgcc
     1921 ttgctgaaaa tcaacgccga cggcagcgaa accccggtgc tgaccggcga agcgaagccg
     1981 tgggggcatt atctgcgcta cgattaccgc accttcgact tttcgtccgt caccgcgccg
     2041 ggcctgtacg agatcgccta tgaggggcag aaaac
//
LOCUS       DASVLL010000371         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_454148,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000371 DASVLL010000000
VERSION     DASVLL010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454148"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..>1661
                     /gene="egtB"
                     /locus_tag="VF402_06175"
     CDS             3..>1661
                     /gene="egtB"
                     /locus_tag="VF402_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX7799916.1"
                     /translation="MNRPIGKTSSVSLSDRYAGVRARTLSLVGRLSAEDMTVQSMPDA
                     SPIKWHLAHTTWFFETFLLADQPGYTPFDPAFAFLFNSYYEALGARQPRAERGLLTRP
                     PLDDVLAYRRHVDRHMALLLAAGRADERLELGLAHEEQHQELMLMDGLHLFSRSPLSP
                     AFLPDWPADAQGRKGHFQLRPGGLVEIGHAGDGFAFDNEAPRHKVWLQPFEISDRLAR
                     NAEWLDFMADGGYRRPELWLSDGWAAVQAQGWRAPLYWRQHKDGWREMTLGGPRPLAP
                     DAPVTHVSFYEADAFARWAGARLPTEAEWEMAAGDGVLDQVWDVGWQWTGSAYLPYPG
                     FRPGFRPGAGAVGEYNGKFMSGQMTLRGGGAMTPDGHSRLTYRNFFAPDKRWVRTAVR
                     LARDLGAPTPDSPDGWDCAFAADVVAGLSARQKALPPKYFYDATGSDLFEAICRTPEY
                     YPTRVETALLRDIAAELARDIPPDAVLVEFGSGASDKTQILLAAAARITAYAPIDISE
                     DALRLATLRLARAWPHLAVTPVVGDFTALAALPSALRDRPRIGFF"
BASE COUNT          225 a          591 c          589 g          256 t
ORIGIN      
        1 ccatgaaccg cccgataggc aaaacgtctt ccgtgtccct gtccgaccgc tatgcggggg
       61 tgcgggcgcg cacgctttcc ctcgtcggcc gtctgtcggc cgaggacatg acggtccagt
      121 ccatgcccga cgccagcccg atcaagtggc atctggcgca tacgacctgg tttttcgaaa
      181 ccttcctgct ggccgaccag ccgggctata cgcccttcga tccggccttc gccttcctgt
      241 tcaactccta ttacgaggcg ctgggcgcgc gccagccgcg cgccgagcgc gggctgctga
      301 cgcggccgcc gctggatgac gtcctcgcct atcgccgcca tgtcgaccgc catatggcgc
      361 tgctgctggc ggcgggccgg gcggacgagc ggctcgaact ggggctggcg catgaggagc
      421 agcatcagga gctgatgctg atggacgggc tgcacctgtt cagccgctcg ccgctcagcc
      481 ccgccttcct gcccgactgg cccgccgacg cgcagggccg caagggtcat ttccagcttc
      541 ggcccggcgg tctggtcgag atcggccacg cgggcgacgg cttcgccttc gacaacgaag
      601 cgccgcgcca caaggtctgg ctgcaaccgt tcgagatcag cgaccggctg gcgcgcaacg
      661 ccgaatggct ggatttcatg gcggacggcg gttaccggcg gccggagctg tggctgtcgg
      721 acggctgggc cgccgttcag gcgcagggct ggcgcgcgcc gctttactgg cgccagcata
      781 aagacggctg gcgcgagatg acgctgggcg ggccgcggcc gctggcgccc gacgcgccgg
      841 tcacccatgt cagcttctac gaggccgacg cgttcgcgcg ctgggccggg gcgcggctgc
      901 cgaccgaggc cgaatgggag atggcggcgg gcgacggcgt gctggatcag gtctgggacg
      961 tcggctggca atggacgggc agcgcctacc tgccctatcc cggcttccgg cccggcttcc
     1021 ggcccggcgc gggcgcggtc ggcgaataca acggcaagtt catgagcggc cagatgacgc
     1081 tgcgcggcgg cggcgccatg acgcccgacg gccattcgcg cctcacctat cgcaacttct
     1141 tcgcgccgga caagcgctgg gtccgcacgg cggtgcggct ggcgcgcgac ctcggcgcgc
     1201 cgacgcccga cagccctgac ggctgggact gcgccttcgc cgccgacgtg gtcgcgggct
     1261 tatcggcgcg gcagaaggcg ttgccgccca aatatttcta tgacgccacc ggttcggacc
     1321 tgttcgaagc catctgccgc acgccggaat attatccgac gcgcgtcgag acggcgctgc
     1381 tgcgcgacat cgcggcggag ctggcccgcg acattccgcc cgacgccgtg ctggtcgagt
     1441 tcggcagcgg cgccagcgac aagacgcaga tcctgctggc ggcggcggcg cggatcaccg
     1501 cctacgcgcc catcgacata agcgaggacg cgctcaggct ggcgacgctc aggctggcgc
     1561 gcgcctggcc gcatctggcg gtgacgccgg tggtcggcga cttcaccgcc ctcgcggcgc
     1621 tgccgtcggc cctgcgcgac cggccgcgga tcggcttctt t
//
LOCUS       DASVLL010000372         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_237650,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000372 DASVLL010000000
VERSION     DASVLL010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237650"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VF402_06180"
     CDS             complement(<1..666)
                     /locus_tag="VF402_06180"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEX7799917.1"
                     /translation="MAMADSSNKGPKLPPANRPAERRERGGWLSKIAPGVSKFVSRRE
                     TPENLWVKCPDTGEMIYRSDLDASFWVTPSGYHMRIGASQRLKYTFDDGQWEALDAPD
                     VAEDPLQFSDGKSYTERLKAARAKTGARDVMAIGYGKVQGVDCIVLVQDFAFMGGSLG
                     TAAGEAFVAAARYAIGRKCPMVCFTAAGGARMQEGALSLMQMARTTLALQELKAAQLP
                     YIVV"
     gene            complement(684..1502)
                     /gene="trpA"
                     /locus_tag="VF402_06185"
     CDS             complement(684..1502)
                     /gene="trpA"
                     /locus_tag="VF402_06185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7799918.1"
                     /translation="MGVSRIDARFNELKRDNKAAFVAYIMAGDPDLETSLRTLQGLPA
                     AGADLIELGFPFSDPMAEGPTIQRAAERALKNGVKIAHVFELVKRFRETDRTTPIILM
                     GYMNPVENRGYEGWARAMAECGADGLIVVDCPPEEADGLTDALDAHDLALIRLATPTT
                     DAARLETVVRRTRGFIYYVSVTGVTGVKEADAAGVAGQVARVREAAHLPVAVGFGIRT
                     PQRAAEIARVADAAVVGSALVDEINTSLLENAAADTKVLEKAAVLAQAVHGARL"
     gene            complement(1505..>1695)
                     /locus_tag="VF402_06190"
     CDS             complement(1505..>1695)
                     /locus_tag="VF402_06190"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519031.2"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7799919.1"
                     /translation="LEAFQLTSKLEGIIPALESSHALARVGDLAKEVGKGGVVVLNLS
                     GRGDKDIFTVAGALGEGM"
BASE COUNT          278 a          589 c          546 g          282 t
ORIGIN      
        1 caccacgata tagggcagtt gcgccgcctt aagttcctgg agcgccagcg tcgtgcgcgc
       61 catctgcatc agcgacaagg cgccttcctg catccgcgcg ccgcccgccg ccgtgaaaca
      121 gaccatcggg catttgcgcc cgatcgcgta gcgcgccgcg gcgacgaagg cctcgcccgc
      181 cgccgtgccg agactgccgc ccatgaaggc gaagtcctgc accagcacga tgcagtccac
      241 gccctgcacc ttgccgtagc cgatggccat cacgtccctg gcgccggtct tggcgcgcgc
      301 ggccttgagg cgctcggtat aggatttacc gtcggagaat tgcagcggat cctcggccac
      361 gtccggcgcg tcgagcgctt cccactggcc gtcatcgaag gtgtatttca gccgttgcga
      421 cgcgccgatg cgcatatgat agccggacgg cgtgacccag aacgaggcgt cgaggtccga
      481 acggtagatc atctcgccgg tgtcggggca cttgacccag aggttttccg gcgtctcgcg
      541 gcgcgaaacg aacttcgaca cgccgggcgc gatcttggac agccacccgc cgcgttcccg
      601 gcgctccgcc ggacggttgg cggggggcag tttcgggcct ttgttcgagg aatcggccat
      661 agccatagaa tgctccgtaa accttacagg cgggcgccgt ggacagcctg agccaaaacg
      721 gccgcctttt ccagcacttt tgtgtcagca gccgcgtttt cgagtaaaga agtgttgatc
      781 tcatcgacca gcgccgaacc caccacggcg gcgtccgcga cgcgggcgat ttcggcggcg
      841 cgctggggcg tgcggatgcc gaagccgacc gcgaccggca gatgcgccgc ttcgcgcacg
      901 cgcgcgacct gaccggccac gcccgccgcg tcggcctcct tcacgccggt cacgccggtc
      961 accgagacgt aatagatgaa gccgcgcgtg cgccgcacca ccgtctcaag acgcgccgcg
     1021 tccgtggtcg gggtcgccag ccggatcagg gccagatcgt gcgcgtcgag cgcgtcggtc
     1081 aggccgtcgg cctcctccgg cgggcaatcg accacgatca ggccgtcggc gccgcactcg
     1141 gccatcgccc tggcccagcc ctcatagccg cgattttcga ccggattcat atagcccatc
     1201 aggatgatcg gcgtcgtccg gtccgtctcg cgaaagcgct tcaccagttc gaagacatgc
     1261 gcgatcttca cgccgttctt caaggcccgt tcggcggcgc gctggatcgt cggcccttcg
     1321 gccatcgggt cggagaacgg aaagcccagc tcgatcaggt cggccccggc ggcgggcagg
     1381 ccctgcaagg tccgcagcga ggtttccaga tcgggatcgc ccgccatgat ataggcgacg
     1441 aaggcggcct tgttgtcgcg cttcagctca ttgaagcgcg cgtcgatgcg agaaacgccc
     1501 atgattacat tccttcccca agcgcgccgg cgacggtgaa aatgtccttg tcgccgcggc
     1561 cggacaggtt gaggacgacg acgccgccct tgccgacctc cttggccaga tcgccgacgc
     1621 gcgccagggc gtgcgaggat tccaacgccg ggatgatgcc ttccagcttc gaggtcagtt
     1681 ggaacgcttc cagcg
//
LOCUS       DASVLL010000373         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_237686,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000373 DASVLL010000000
VERSION     DASVLL010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237686"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VF402_06195"
     CDS             <1..657
                     /locus_tag="VF402_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7799920.1"
                     /translation="ELFIVEGDSAGGSAKQGRNRNTQAILPLRGKILNVASATGDKAR
                     ANQELSDLGLALGVQPGAKFREDDLRYERIIIMTDADVDGAHIASLLITYFYRTMPSM
                     IRNGNLYLAMPPLYRLSLGGKTMYARDDAHRDELIAKEYKGKRPEIGRFKGLGEMMPG
                     QLKETTMDPRTRTLARVTLPHDEEDIEDLVETLMGRKPELRFRYIQDHAEFAVADLDV
                     "
     gene            733..2181
                     /locus_tag="VF402_06200"
     CDS             733..2181
                     /locus_tag="VF402_06200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7799921.1"
                     /translation="MTKFTDLGLGAEVLKAVAETGYDTPTAIQEQAIPVALTGLDVLG
                     IAQTGTGKTAAFTLPMIERLAAGRSRARMPRAIVLAPTRELADQVSEAFEKYAKYHKL
                     NWALLIGGVSMADQIAKLDKGVDVLICTPGRLLDLFDRSKVMLNGVQIMVVDEADRML
                     DMGFIPDIERIFKLTPATRQTLFFSATMPPEITRLTEQFLKNPVRIEVSRPASTNENI
                     TQYVLRVPHRDARAKRIALRALLKTLDIKNGIVFCNRKTDVDIVAKSLSKHGFNAAPI
                     HGDLDQALRMKTLDVFRKGDLTLLVASDVAARGLDIPDVGHVFNFDAPIHADDYVHRV
                     GRTGRAGRSGEAYLLLSPADEKNYDKVVKLIKKEPETLALDVDYNDLSETPRAPRRDS
                     GRDSGKGRGGDRKRDHKERPARRAAEAAVEEKITAPEEKKPQPVREAPAVAAAPSPRK
                     KKSGKTNEKPQDDTAFGDHVPAFILRAAPIKG"
BASE COUNT          470 a          749 c          702 g          354 t
ORIGIN      
        1 gaactgttca tcgtcgaagg cgattcggcc ggcggctcgg ccaagcaggg ccgcaaccgc
       61 aatacgcagg ccattctgcc cctgcgcggc aagattctca acgtcgcctc ggccaccggc
      121 gacaaggcgc gcgccaatca ggagctgtcc gatctcggcc tcgcgctcgg cgtgcagccc
      181 ggcgccaaat tccgcgagga cgatctgcgc tatgagcgca tcatcatcat gaccgacgcc
      241 gacgtcgacg gcgcccacat cgccagcctg ctgatcacct atttctaccg caccatgccg
      301 tcgatgatcc gcaacggcaa cctgtacctg gccatgccgc cgctgtaccg cctgagtctg
      361 ggcggcaaga cgatgtacgc gcgcgacgac gcccaccgcg acgaattaat cgctaaggaa
      421 tacaagggca agcgcccgga gatcggccgt ttcaagggcc tcggcgaaat gatgccgggc
      481 cagctcaagg aaacgacgat ggaccccagg acgcgcaccc tggcgcgcgt caccctgccg
      541 cacgacgagg aggatatcga ggatctggtc gagaccctga tggggcgcaa accggaactg
      601 cgtttccgct atattcagga ccacgccgaa ttcgccgtcg ccgatctgga cgtttaggac
      661 gtgaatacgc agaaaaacct gttaattccg gcgatttctg ctatgtgacg cgcattcaca
      721 attgcaggca tcatgacaaa atttaccgat ctgggtctgg gcgcggaagt gctcaaggcc
      781 gtggctgaaa caggttacga cacgccgacg gcgattcagg agcaggccat accggtcgcg
      841 ctgaccggtc tggacgtgct cggcatcgcc cagaccggca cgggcaagac ggcggccttc
      901 accctgccga tgatcgagcg tctggcggcg ggccgttcgc gcgcgcgcat gccgcgcgcc
      961 atcgtgctgg cgccgacgcg cgaactggcc gaccaggtgt ccgaggcgtt cgagaaatac
     1021 gccaagtatc acaagctgaa ctgggcgctg ctgatcggcg gcgtgtcgat ggccgaccag
     1081 atcgccaagc tggacaaggg cgtggacgtg ctgatctgca ctccgggccg cctgctggac
     1141 ctgttcgacc gctccaaggt catgctcaac ggcgttcaga tcatggtggt cgacgaagcc
     1201 gaccgcatgc tggacatggg cttcatcccc gacatcgagc gcatcttcaa gctgactccc
     1261 gcgacgcgcc agaccctgtt cttctcggcg accatgccgc cggaaatcac gcgcctgacc
     1321 gaacagttcc tgaagaatcc ggtgcgcatc gaggtgtcac gtccggcctc gaccaacgag
     1381 aacatcacgc aatacgtgct gcgcgtgccg caccgcgacg cgcgcgccaa gcgcatcgcg
     1441 ctgcgcgccc tgctgaaaac gctggacatc aagaacggca tcgtgttctg caaccgcaag
     1501 accgatgtcg atatcgtcgc caagtcgctg agcaagcacg gcttcaacgc cgcgccgatc
     1561 cacggcgatc tcgatcaggc gttgcgcatg aagacgctgg acgtcttccg caagggcgac
     1621 ctgaccctgc tggtggcgtc cgacgtcgcc gcgcgcggcc tcgacatccc cgatgtcggc
     1681 catgtgttca atttcgacgc gccgatccac gccgacgact acgttcaccg cgtcggacgc
     1741 accggacgcg cgggccggtc gggcgaagcc tatctgctgc tgtcgcccgc cgacgagaag
     1801 aattacgaca aggtcgtcaa gctcatcaag aaagagccgg aaaccctggc gctcgacgtc
     1861 gattacaacg acctgagcga gacgccgcgc gcgccgagac gagattcggg gcgcgattcc
     1921 ggcaagggcc gtggcggcga ccgcaagcgt gatcacaagg aaaggcccgc gcgccgcgcc
     1981 gccgaggcgg cggtcgagga aaaaattacc gcgcctgagg aaaaaaagcc acagccggtc
     2041 agggaagcgc ccgccgtcgc cgccgcgccg tcgcctcgca agaaaaagtc aggaaaaaca
     2101 aacgaaaagc cgcaggacga tacggctttc ggtgatcacg ttccggcttt cattttgcgc
     2161 gccgccccga ttaagggcta gggccgcgga ttttgccgtc gcggccgccg gcaaagcgaa
     2221 cggcgttaac cgcctgtcag aaaagttgcg ctaaggtgcc gctgttgcgc agaac
//
LOCUS       DASVLL010000374         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_237788,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000374 DASVLL010000000
VERSION     DASVLL010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237788"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1645
                     /locus_tag="VF402_06205"
     CDS             <1..>1645
                     /locus_tag="VF402_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEX7799922.1"
                     /translation="APGVARLGIPAQWQTDAGIGVATQHDATHWRERTALPSGLGTAA
                     TWNTRLAYEGGAMIGAEARDSGFNVLLAGGVNLMRDPRDGRNFEYGGEDPLLAGRMVG
                     AQIAGVQSNRIVSTVKHFAFNDQETGRHFVSSNLDHDQAHMSDLLAFQFAIEASDPGS
                     VMCAYNRVDEDYACENDWLLNRVLKGDWGYKGYVMSDWGAVYSTVKAANAGLDQESAG
                     DAFDARPFFKDDLKQAVADGRVSEARLDDMVRRILRSLYATGVMDAPPVAEKPIDLAA
                     HGRVSQADAEEAIVLLKNDPVGGQSLLPLARTAKHIVIIGGHADVGVLSGGGSSQVHA
                     KGGNAVPGLGSPDFPGPIYYFPSSPMQAIKARLPDAQIRFVSGDDPAAAAQAAKDADL
                     AIVFADQWTGEDHDFSITLPGQQDATVAAVAAANPRTVVVLETGGPVLTPWADKVPAL
                     LEAWYPGTNGGEAIARVLFGDVDASGRLPATFPASLDQLPHPALNDNNGKPHQPFDMT
                     YDEGAAVGYKWFDKKGLTPRFPFGYGLSYTHFRYSGLSAA"
BASE COUNT          264 a          582 c          555 g          244 t
ORIGIN      
        1 gcgccgggcg tggcgcggct ggggattccg gcgcaatggc agaccgatgc gggcataggc
       61 gtggcgaccc agcacgacgc gacccactgg cgcgaacgca ccgccctgcc ctccggcctc
      121 ggcacggcgg cgacgtggaa cacgcggctg gcctatgagg gcggcgcgat gatcggcgcc
      181 gaggcgcgcg attccggctt caacgtcctg ctggcgggcg gagtcaacct gatgcgcgac
      241 ccgcgcgacg gacgcaattt cgagtatggc ggcgaggatc cgctgctggc cgggcgcatg
      301 gtcggcgcgc agatcgcggg cgtgcagtcg aaccgcatcg tctcgaccgt caagcacttc
      361 gccttcaacg atcaggagac gggccgccac ttcgtcagct cgaacctcga ccacgatcag
      421 gcgcacatgt ccgacctgct ggccttccag ttcgccatcg aggcgtccga tcccggttcg
      481 gtgatgtgcg cctataaccg cgtcgacgaa gattatgcgt gcgagaacga ctggctgctc
      541 aaccgggtgc tgaagggcga ctggggctat aagggctatg tgatgtccga ctggggcgcg
      601 gtctattcca ccgtcaaggc cgcgaacgcc ggtctggatc aggaatcggc gggcgacgcc
      661 ttcgacgcca ggcccttctt caaggacgat ctgaaacagg cggtcgcgga cgggcgcgtg
      721 tccgaggcgc ggctcgacga catggtccgc cgcatcctgc gcagcctcta cgccaccggc
      781 gtgatggacg cgccgccggt ggcggaaaag cccatcgacc tcgccgccca cggcagggtc
      841 agccaggccg acgccgagga ggccatcgtg ctgctgaaaa acgatccggt cggcgggcag
      901 agcctgctgc cgctggcgcg gacggcgaaa cacatcgtca tcatcggcgg ccacgccgat
      961 gtcggcgtcc tgtcgggcgg cggctcgtcg caggtccacg ccaagggcgg caacgcggtt
     1021 ccgggtctgg gctcgcccga ctttcccggc ccgatctact atttcccgtc ctcgccgatg
     1081 caggcgatca aggcgcgcct gccggacgct cagatccgct tcgtcagcgg cgacgacccc
     1141 gccgccgccg cgcaggccgc gaaggacgcc gatctggcga tcgtgttcgc cgatcagtgg
     1201 accggcgagg accacgactt ctccatcacc ctgcccggcc agcaggacgc caccgtcgcg
     1261 gcggtcgccg ccgccaatcc gcgcaccgtc gtcgtgctgg aaaccggcgg gccggtgctg
     1321 acgccgtggg cggacaaggt tccggccctg ctcgaagcct ggtatccggg cacgaacggc
     1381 ggcgaagcca tcgcgcgcgt gctgttcggc gatgtcgacg cctccggccg cctgcccgcc
     1441 accttccccg cttcgctgga ccagttgccg cacccggccc tcaacgacaa taacggcaag
     1501 ccgcatcagc ccttcgacat gacctacgac gaaggcgcgg cggtcggcta taaatggttc
     1561 gacaagaagg gcctgacgcc gcgcttcccg ttcggctacg gcctcagcta cacgcatttc
     1621 cgctacagcg gcctgagcgc cgcct
//
LOCUS       DASVLL010000375         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_237893,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000375 DASVLL010000000
VERSION     DASVLL010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237893"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VF402_06210"
     CDS             <1..1459
                     /locus_tag="VF402_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7799923.1"
                     /translation="LMGRTRVTLIESPDIAAIGVGEGAFPTLRSTLRDLGVDEADFMR
                     RASATFKQGVRFNNWVRTPRDGEAEHFFHPFEAPYYDDGAALAYWLRLPPDARPPFAQ
                     AMTFQKRVAEAGRGPKRPHEGDFTGPLNYAYHIDSSQLAGMLAERARALGVRHIADAV
                     THVEVAPDGAVAQVHTRAHGAHAADLYIDCTGFRAELIGQALKAPFRSIRDVLFADRA
                     VTCRAAYPRPDAPLESYTQANAHAAGWSWDIGLEAMRGVGYVYSSAHSDDAQAEAVLR
                     DYMRGHEIEPRVIRFEPGWRETPWVRNCVAVGLSGGFVEPLEATGIVLIELAASMIAD
                     LLPASGPVAAPAARFNALMRERYENIANFLKLHYVLSRRDEPFWRDNRDPASIPERLR
                     DLLDQWRHRPPSRFDFRLDAETFAWFNYQYILYGMGFETRVAGTDNDVAASAEAQRML
                     NRIRQFGERATLDLPPHRTLIEQIYAHGFRETAQT"
     gene            1560..1898
                     /locus_tag="VF402_06215"
     CDS             1560..1898
                     /locus_tag="VF402_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX7799924.1"
                     /translation="MGAATALMGENAVMACTKAVERTVHRHLDDQIRFLRGRDEDLRD
                     LIAHIQSEEAAHLAYAEAHMRKSAAARLIEALVVSATESVIWLSTQGDVGRMARALEG
                     DARCAGSSTP"
     gene            1874..>2441
                     /locus_tag="VF402_06220"
     CDS             1874..>2441
                     /locus_tag="VF402_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX7799925.1"
                     /translation="MCGQFDALAQLGFLQELLGVKPVALPPLPRRVAPSAGAPIVIEN
                     PASRAFEALPARFGLVPSWYRGSLKDWKAATFNARVEEAVAKPAFKGAWRYRRALIPA
                     EAFYEWSGPKTDRRRFRVTRDDAEPLMFAGLWEEACLAEGELWSFAVLTRPAGADMAA
                     IHDREPLVLPPESWTRWLRREAVDLTRPA"
BASE COUNT          379 a          903 c          790 g          369 t
ORIGIN      
        1 cctgatgggg cgcacgcgcg tcaccctgat cgaatcgccc gacatcgcgg ccatcggcgt
       61 cggcgaaggc gcctttccga ccctgcgttc gaccctgcgc gatctcggcg tcgacgaggc
      121 cgacttcatg cgccgcgcct cggccacctt caagcagggc gtgcgcttca acaactgggt
      181 caggacgccg cgcgacggcg aggcggagca tttcttccac ccgttcgagg cgccctatta
      241 cgacgacggc gccgcgctgg cctactggct gcggctgccg cccgacgcgc gcccgccctt
      301 cgcccaggcc atgaccttcc agaagcgcgt cgccgaggcc ggacgcggcc ccaagcgccc
      361 gcacgaaggc gacttcaccg gcccgctcaa ctacgcctac catatcgatt cctcgcaact
      421 ggccgggatg ctggccgagc gcgcgcgggc tctgggcgtg cgccatatcg cggacgccgt
      481 cacccatgtc gaggtcgcgc ccgacggcgc cgtcgcacag gtgcatacgc gggcgcacgg
      541 cgcgcacgcc gccgacctct atatcgactg caccggcttt cgcgccgaac tgatcggtca
      601 ggcgctgaag gcgccgttcc gctcgatccg cgacgttctg ttcgccgacc gcgccgtcac
      661 ctgccgcgcc gcctatccgc gcccggacgc gccgctggaa agctacaccc aggccaatgc
      721 gcacgcggcg ggctggagct gggacatcgg cctcgaagcc atgcgcggcg tcggctacgt
      781 ctattccagc gcccacagcg acgacgcgca agccgaggcg gtcctgcgcg actatatgcg
      841 cggccacgag atcgagccgc gcgtgatccg cttcgagccg ggctggcgcg aaacgccgtg
      901 ggtgaggaac tgcgtcgcgg tcgggctgtc cggcggcttc gtcgagccgc tggaggccac
      961 cggcatcgtg ctgatcgagc tggcggccag catgatcgcc gacctcctgc ccgcctccgg
     1021 cccggtcgcc gcccccgccg cgcgcttcaa cgccctgatg cgcgagcgtt acgagaacat
     1081 cgccaacttc ctgaagctgc actatgtgct gagccgccgc gacgagccgt tctggcgcga
     1141 caaccgcgat ccggcctcga tccccgaacg cctgcgcgac ctgctggacc agtggcgcca
     1201 ccgcccgccc agccgcttcg acttccggct ggacgccgag accttcgcct ggttcaacta
     1261 tcagtacatt ctctacggca tgggcttcga gacgcgcgtc gccgggacgg acaacgacgt
     1321 cgccgcctcc gccgaggccc agcggatgct gaaccgcatc cgccagttcg gcgagcgcgc
     1381 cacgctggac ctgccgccgc accgcacgct gatcgagcag atctatgcgc acggcttccg
     1441 cgagacggcc cagacctaga gcgtttcccc aaaagcggtt ccactttttg gatcaggaag
     1501 agcgacaaaa caaagactta gagcgccaat ctgattccat cagattgacg ctctaagtcc
     1561 tgggcgccgc gaccgccctg atgggcgaaa acgccgtcat ggcctgcacg aaagccgtgg
     1621 agcgcaccgt gcatcgccat ctggacgacc agattcgctt cctgcgcggc cgcgacgagg
     1681 acttgcgcga cctgatcgcg cacattcaga gcgaggaggc cgcgcacctc gcctacgccg
     1741 aggcgcatat gcggaagtcc gccgccgccc gcctgatcga ggcccttgtc gtcagcgcga
     1801 ccgaatcggt catatggcta tccactcagg gcgatgtcgg ccgcatggcg cgcgccctgg
     1861 agggagatgc gcgatgtgcg ggcagttcga cgccttagcc caactggggt ttcttcagga
     1921 gttgctgggc gtgaagcccg tggccctgcc gcctttgccg cgccgcgtcg cgccgtccgc
     1981 gggcgcgccc atcgtcatcg agaaccccgc aagccgcgcg ttcgaggcgc ttccggcgcg
     2041 tttcgggcta gttccgtcat ggtatcgcgg ttcgctgaag gactggaagg ccgcgacctt
     2101 caacgcccgc gtcgaggaag ccgtcgcaaa accggccttc aagggcgcgt ggcgctaccg
     2161 ccgcgccctg attcccgccg aagccttcta cgaatggtcc gggccgaaaa cggaccgccg
     2221 ccgcttccgc gtcacgcgcg acgacgcgga gccgctgatg ttcgcaggct tgtgggagga
     2281 ggcctgtctg gccgagggcg agctatggag cttcgccgtc ctgacccgcc cggcgggcgc
     2341 ggacatggcc gcgatccacg accgcgagcc gcttgtcctg ccgccggaaa gctggacgcg
     2401 ctggctgcgg cgcgaagccg tcgacctgac gcgccccgcc c
//
LOCUS       DASVLL010000376         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_185178,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000376 DASVLL010000000
VERSION     DASVLL010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185178"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VF402_06225"
     CDS             complement(<1..367)
                     /locus_tag="VF402_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799926.1"
                     /translation="MSLAATFGIGHASASALAIADARAIAGHAASTARPTATDYAGLR
                     AKWSVMQTGKGNYNPIAAPYAAYISAIDAEARGWYSTLDTAPVPGGSLWSDTLAGTNE
                     VNTRVSFDRLRILAIAYATD"
     gene            335..607
                     /locus_tag="VF402_06230"
     CDS             335..607
                     /locus_tag="VF402_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799927.1"
                     /translation="MTDAEGGGQRHAGAGEKKSTPRNRRACIGACIEACIGVVHVASL
                     LNSALFRSLRQPDLILGHHIGAQDGRDNARHLSHAKYILVIENICQ"
     gene            1124..>1850
                     /locus_tag="VF402_06235"
     CDS             1124..>1850
                     /locus_tag="VF402_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799928.1"
                     /translation="MSDRPGKQYFWDASMMAVVWALLEPEGMKMSLRAWLSGDLLHAY
                     GLNMTTGAPMGPTWYAFSATNVFNTALTYIEVTGDRAFLEEDMPSSGKSVLWRLDDIA
                     MLWKEKVRPGQILASFGGNGNLLESGAPTYLGAVASLNAQSVGMMRDMARLHRAFGNN
                     DRAGLLDAAADALLPAVLALYKPGEGVWFSMTDDGQRVEHRHVVDFIYVSQALASDIP
                     PFMRREMRDFVQRELMMKNWLRAQ"
BASE COUNT          348 a          548 c          587 g          367 t
ORIGIN      
        1 cgtcggtggc ataggcgatg gccaggatgc gcagccggtc gaacgacacg cgggtattga
       61 cctcgttcgt cccggccaat gtgtcgctcc acagcgatcc gcccggaacc ggcgccgtgt
      121 cgagcgtcga ataccagccc ctggcctcgg cgtcgatggc ggaaatatag gcggcgtagg
      181 gagcggcgat ggggttgtaa ttgcccttgc ccgtctgcat caccgaccac ttggcgcgca
      241 atcccgcata gtcggtggcg gtcggacggg cggtcgaggc cgcatgcccg gcgatggcgc
      301 gcgcatcggc aatcgccagc gccgaggcgc tcgcatgacc gatgccgaag gtggcggcca
      361 acgacacgcc ggcgccggtg aaaagaaaag cacgccgcga aaccggcgcg cctgtatcgg
      421 ggcctgtatc gaggcctgta tcggggtcgt tcatgtcgcc tcgctgttga actcggccct
      481 gttccgcagt ttgcgccagc cggatttgat tttgggacac cacatcggcg cccaggatgg
      541 acgcgacaat gcgcgccatc tctcacatgc gaaatatatt ctcgtcatcg agaatatttg
      601 tcaataagct gaaaatatgc actgcgcttc cattccacga cttgtggggg gggaaacgct
      661 tgttcaccgg cgtagaagcg cctgtttgcg acctgcgccg ctttttcaga gctggaagca
      721 tttaacccgc ggcgctataa acccttggcc agcttgacga gtttgttatc gccgccgatc
      781 ggccatggat tccaagcatg atagccgcag cgcgcgccgg gcgccgggcg ccgggcgccg
      841 aattaatgac gagccccgcg aaaattcagg aaagcgatac cggcatatcc atatccccgc
      901 cacaacggcc tgaccctggc tctgcgcgag atgggggcgc tttgagcgcg ccttgctgtg
      961 gaacatggtt tatcccggtg ttgtcaatcg tcatttttcc gggcacttgc cgctgttcgt
     1021 cacatcggac gtcggtttga gacgcatcta ctatatgagc gccctgacag ttttgtgttg
     1081 cgagcgatcg caattcaggg tcagcccgcg agattttttg acattgagcg acagacccgg
     1141 aaagcagtat ttctgggacg cctcgatgat ggccgtggtc tgggcgctgc tggagccgga
     1201 aggcatgaaa atgtccttgc gggcgtggct ttcgggcgac ctgttgcacg cctatggcct
     1261 gaacatgacc accggcgccc ctatggggcc gacatggtac gccttcagcg ccacgaacgt
     1321 cttcaacacg gcgctgacct acatagaagt gaccggcgat cgcgcgtttc tggaggaaga
     1381 catgccttcc tccggcaaga gcgtcctgtg gcgcctcgac gacatcgcca tgctgtggaa
     1441 agaaaaggtc aggccgggac agatactggc cagtttcggc ggcaacggca atctgctgga
     1501 aagcggcgcc ccgacttatc ttggagcggt ggcgtcctta aacgcgcaaa gcgtcggcat
     1561 gatgcgcgac atggcgcggc tgcaccgggc gttcggaaat aatgaccgcg ccggactcct
     1621 tgatgcggcg gccgacgccc tgctgccggc cgtgcttgcg ctttataagc cgggcgaggg
     1681 cgtctggttc agcatgaccg atgacgggca gcgcgtcgaa caccgccatg tcgtcgactt
     1741 catctatgtc agccaggccc tggcgtcgga tattccgccg ttcatgcggc gggagatgcg
     1801 tgattttgtc caacgcgaac tcatgatgaa aaactggctg cgggcgcagt
//
LOCUS       DASVLL010000377         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_24065, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000377 DASVLL010000000
VERSION     DASVLL010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24065"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /gene="sfsA"
                     /locus_tag="VF402_06240"
     CDS             <1..565
                     /gene="sfsA"
                     /locus_tag="VF402_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEX7799929.1"
                     /translation="ALVTRLPGSNRKLGYRLEALRAAADFGATWVGVNTQWPNRLIAQ
                     AIAAGLIPALSGYAQARPEVRYGAGSRIDLLASGHAGRPDAFIEIKNVHLSRAPGLAE
                     FPDCVTARGARHLHELAQVAAAGHRAVVAFCIQRDDVTAFDVARDCDPAFGRAFDAAR
                     QAGVEMLAFSFCVSPQGLAFAREIPVL"
     gene            complement(717..2447)
                     /locus_tag="VF402_06245"
     CDS             complement(717..2447)
                     /locus_tag="VF402_06245"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX7799930.1"
                     /translation="MTTRRDVWRGAGALAALAVLPGTGLAASPVIVRVPIGFARNGMP
                     LLRLTLDGKGPYRFAIDTGAFAPCIRESLAKDLRLRRGGSIRTASLKGDEADYIYAAE
                     NVVIGGGLPFPKMSFIGFEKFPSSDMDGVLPASFLTSLPSQLDYERREIRYYLNGADM
                     DLDGFVRADAFFQAQKDNYAEKVFLTFQLDGRRLVCIVDTGAMAAIAVSGAYVGAHHL
                     WDKYKVLRESDSVGANGQSVHTRIVVAPDLTLGSLHLNAAPVTLIDPHAWDSLGEEDV
                     DGLVGTPLLRKFTLAFTGHKELYLKPNAGFAQISGRMPDQAVPDDAPAGAIPILYGEQ
                     HRILVPAKLGDDPAFPFMLATGQSECAISSREAEAHHIPALPDGGYDGGGLGFGEVRL
                     PHLKLTAHGGPAALGLDFLRGAPVSLDFDADLLTFHTDAPPDMSGYNRSDAARQPNPR
                     GGEGLYGTARLFGADLRCRIDTAFPYGLYLPPHTVAARQFWDRFPKARDAVMASAGRK
                     FATREVLAGPFSLAGFDVRPAPLILADPKAVGAPDDDAVLGMAVLQRFNFVFDRDGAV
                     WLRPNARFKA"
     gene            2622..3077
                     /locus_tag="VF402_06250"
     CDS             2622..3077
                     /locus_tag="VF402_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7799931.1"
                     /translation="MSEPVSDLETHLGYWLRYVSNHVSQAFARKVEARGVTVAEWVVL
                     RELYDADDALPARIAERIGMTRGAITRLADRLLAKALIERRDNPQDGRSQLLRLSETG
                     RALVPELARLADRNDAEFFDHLTPDDRARLEQILRGVVERRSLRAAPID"
     gene            3167..3574
                     /locus_tag="VF402_06255"
     CDS             3167..3574
                     /locus_tag="VF402_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HEX7799932.1"
                     /translation="MSDALPMPVLDAIAAVVEGSDSGVMPFPEVVRRLAEAGVESYYA
                     DMRRSERIFFMPDGRSRCVASRLSHEAAAPVFSPAGVKAAIADIQQKRIDYPAFCERI
                     LAAGCCAYVVTLLGRRAVYMGRDGETYVEPFPT"
     gene            complement(3770..>4269)
                     /locus_tag="VF402_06260"
     CDS             complement(3770..>4269)
                     /locus_tag="VF402_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HEX7799933.1"
                     /translation="FFQPGPDEGRRIRRDGETLHLAASGTAPKDSAPLLLIAGDPAYE
                     MQCEIEVDDGARAGLILFYDSGLYGGLGFDDKAFVTHQYGLERGRPANPYGRRLFLRL
                     RNDRHIVQMHTSGDGRDWTLFDRGMEVSGYHHNVRGGFLMLKPGLYAAGEGEARFRDF
                     RYRAL"
BASE COUNT          729 a         1483 c         1374 g          683 t
ORIGIN      
        1 cgccctcgtc acacgcctgc cggggtcgaa ccgcaagctc ggctaccggc tcgaagcctt
       61 gcgggcggcg gcggacttcg gcgcgacttg ggtcggcgtc aatacgcaat ggccgaaccg
      121 cttgatcgcg caggcgatcg cggcgggcct gatccccgcc ctgtccggct atgcgcaggc
      181 gcggcccgaa gtgcgctacg gcgccggctc gcgcatcgac cttctggcca gcggccacgc
      241 cgggcggccc gacgccttta tcgagatcaa gaacgtccac ctgtcgcgcg cgccggggct
      301 ggccgaattc cccgactgcg tgaccgcgcg cggcgccagg cacctgcacg agctggcgca
      361 ggtggcagcc gccggtcatc gcgccgtagt cgccttctgc atccagcgcg acgacgtgac
      421 cgcgttcgac gtggcgcgcg actgcgatcc ggccttcggg cgcgccttcg acgccgcgcg
      481 acaggcgggc gtcgaaatgc tggcgttcag cttttgcgtc tcgccgcagg gcctggcctt
      541 cgcgcgcgag attccggtcc tttagagcgt tttccgatct gattgcatca gatcggcgct
      601 ctaactttat gcttttacgc gctttttaat ccgaaaagtg cgtcacactt ttcggaaagc
      661 gctctaacgc ctgagaaaaa gcgccggagg caggaccgcc tccggcgcct tgttccttac
      721 gccttgaagc gcgcgttcgg cctgagccag accgcgccgt cgcggtcgaa tacgaagttg
      781 aagcgttgca agacggccat gcccagcacc gcatcgtcgt ccggcgcccc cacggccttc
      841 gggtcggcca ggatcagcgg cgcgggccgc acgtcgaagc ccgccagact gaacggcccg
      901 gccagcacct cgcgcgtcgc gaacttgcgc ccggcggacg ccatgaccgc gtcgcgcgcc
      961 ttggggaagc gatcccagaa ttgccgcgcc gccaccgtat gcggcggcag gtagaggccg
     1021 tacgggaagg ccgtgtcgat gcggcagcgc aggtcggcgc cgaacaggcg cgccgtgccg
     1081 taaagccctt cgccgccgcg cggattcggc tggcgggccg cgtccgagcg gttatagccg
     1141 ctcatatcgg gcggcgcgtc ggtatggaag gtcagcaggt cggcgtcgaa atcgagcgac
     1201 acgggcgcgc cgcgcaggaa atccagcccc agcgccgccg ggccgccatg cgccgtcagc
     1261 ttcaggtgcg gcaggcgcac ctcgccgaag ccgaggccgc cgccgtcata accgccgtcc
     1321 ggcagggccg ggatatgatg cgcctcggcc tcgcggctgg agatggcgca ttccgactgc
     1381 cccgtcgcca gcatgaacgg aaacgccggg tcgtcgccca gctttgcggg aaccagaatc
     1441 cggtgctgtt cgccgtagag gatcgggatc gcgcccgccg gggcgtcgtc gggcacggcc
     1501 tgatccggca tgcggccgct gatctgcgcg aagcccgcgt tcggcttgag gtacagctcc
     1561 ttgtggccgg tgaaggccag ggtgaacttg cgcagcagcg gcgtgccgac cagcccgtcc
     1621 acgtcctcct caccgagcga atcccaggcg tgcggatcga tcagcgtcac cggcgcggcg
     1681 ttgaggtgca ggctgcccag cgtcaggtcg ggcgcgacca cgatccgcgt gtgaacggac
     1741 tgcccgttgg cgccgacgct gtcggattcg cgcaaaacct tatatttgtc ccacaggtga
     1801 tgcgcgccga cataggcgcc gctgacggcg atggccgcca tggcgccggt gtcgacgatg
     1861 cagaccagcc tgcgcccgtc gagttgaaag gtcagaaaga ccttttcggc gtagttgtct
     1921 ttctgcgcct gaaaaaaggc gtcggcgcgc acgaagccgt cgaggtccat gtcggcgccg
     1981 ttgagataat agcggatctc gcgcctctca taatcgagct gcgacggcag cgaggtcagg
     2041 aagctggcgg gcagcacgcc gtccatgtcg gacgacggga acttctcgaa gccgataaac
     2101 gacatcttcg gaaacggcag gccgccgccg atcacgacgt tttcggcggc gtagatatag
     2161 tccgcctcat cgcctttcag gctggcggtg cggatggagc cgccgcgccg cagccgcaga
     2221 tccttggcca gcgactcgcg gatgcacggc gcgaacgcgc cggtgtcgat ggcgaaccgg
     2281 taaggcccct tgccgtccag agtcaggcgg agcagcggca tgccgttgcg ggcgaagccg
     2341 atcggcacgc gcacgatgac cggactggcc gccaaccccg ttcccggcaa aaccgccagc
     2401 gccgccaggg cgcccgcccc ccgccagaca tcgcgtcgcg tcgtcatgat ttccccccgt
     2461 gccgcctgaa aaaagactac aggccgcccc ggaccttagc aagataacgg cggcgctagc
     2521 cggacaaaat aattcataca aaataattcg ggcgaaataa ttgttgacta ggaaacatta
     2581 tggatttagc ttcctggtca acaaaaccgc gcaggccgtc catgtccgaa cccgtatccg
     2641 atctcgaaac gcatctcggc tactggctgc gctacgtctc caaccatgtg tcgcaggcct
     2701 tcgcgcgtaa ggtcgaggcg cgcggcgtca ccgtcgccga atgggtcgtg ctgcgtgagc
     2761 tttacgacgc ggacgatgcg ctgccggcgc gcatcgccga gcgcatcggc atgacgcgcg
     2821 gcgcgatcac caggctggcc gaccggctgc tggccaaggc gctgatcgag cggcgcgaca
     2881 acccgcagga cgggcgctcg caactgctgc gcctgagcga aacgggccgc gccctggttc
     2941 ccgaactggc gcggctggcc gaccgcaacg acgccgagtt tttcgaccat ctgacgccgg
     3001 acgaccgcgc gcggcttgag caaattttgc gcggcgtggt cgaacgacga agcctgcgcg
     3061 ccgcgcccat cgactgaatt ttccccgcgt atccgcatcc tcagcgccgc cgcgacccgg
     3121 cggaagcgcg cccccatgcc tgaccccatg cctgaaagga atctccatgt ccgacgccct
     3181 acccatgccc gtcctcgacg ccatcgccgc cgtcgtcgaa ggctccgatt ccggcgtcat
     3241 gccgttcccc gaagtggtgc ggcgcctcgc cgaagccggc gtcgaatcct actatgccga
     3301 catgcgccgt tccgagcgca tcttcttcat gccggacggg cgctcgcgct gcgtcgccag
     3361 ccgcctgagc cacgaagcgg cggcgccggt cttctccccc gcaggcgtca aggcggcgat
     3421 cgccgacatc cagcagaagc gcatcgacta tcccgccttc tgcgaacgca tccttgccgc
     3481 cggatgctgc gcctatgtcg tcaccctgct gggccgccgc gccgtctata tgggccgcga
     3541 cggcgagact tacgtcgagc ctttcccgac ctgagggtct gaagaaggga atccacagat
     3601 ggacacagat ggacgcagat ttttacggcg cgggacggaa agcgcggcgc gccctcttat
     3661 aataaaggat gcccgaagcg catcgcgatc tattccgcgt tcgccgtgaa aacgccggtc
     3721 agcgccgtaa aaatctgtgt ccatctgtgt ccatctgtgg attccctctt cacaacgccc
     3781 gataccggaa atcgcgaaac cgcgcctcgc cctcgcccgc cgcgtagagg cccggcttga
     3841 gcatcagaaa gccgccgcgc acattatgat ggtagccgga aacctccatg ccccggtcga
     3901 acagcgtcca gtcccggccg tcgccggaag tgtgcatctg cacgatatgc cggtcgttgc
     3961 gcaggcgcag gaacaggcgg cggccatacg gattggcggg ccgcccgcgc tccagcccgt
     4021 actggtgcgt gacaaacgcc ttgtcatcga agcccagtcc gccatagagg ccggaatcgt
     4081 aaaacaggat cagccccgcc cgcgcgccgt catcgacttc gatttcgcat tgcatctcat
     4141 aggccggatc gcccgcgatc agcaaaagcg gcgcggaatc cttcggcgcg gtcccgctgg
     4201 ccgccagatg cagcgtctcg ccgtcgcggc ggatgcgccg cccctcgtcc ggccccggct
     4261 gaaagaagt
//
LOCUS       DASVLL010000378         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_185325,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000378 DASVLL010000000
VERSION     DASVLL010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185325"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..510)
                     /locus_tag="VF402_06265"
                     /pseudo
     CDS             complement(385..510)
                     /locus_tag="VF402_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(547..1938)
                     /locus_tag="VF402_06270"
     CDS             complement(547..1938)
                     /locus_tag="VF402_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7799934.1"
                     /translation="MLKFITLTSAAVLLSACATAPVKQAPLPPKTLIAPAAFAGPQTA
                     AAMPAPASGQWWLIFHDDALDGLIARAMSANNDVALASAHLEQARALLGSARSDLWPQ
                     FGAGYNPSRSTDLQPPGKAANVQAVTADLSYEVDLFGRLNRAARAADDDAQASAALLK
                     DAQLLIQSRVAESYFALRALDEDRAIVADTLGAYRQSLAVTQHRFDEGDVAELDLARM
                     KTEVASTEAAAAALDQQRAQALHALAVLVGEPASGFAMETQPWARQPWADAVPAVPAG
                     LPSDVLNRRPDLIAARASLNAAAQRVGVAKAAWLPSLSLTAQGGYASPKITGLFDPAS
                     TTFTLAAALSQAVFDGGARKAGIDYAKGGLDAAFANYRQTTLTALQEVEDSLSDLDYL
                     RQQQDAETDAVASALRAYDLSQTRYREGSSSQLEVLDAERSLLSIRREALRVRAAQYQ
                     ARIALIRAIGGGW"
BASE COUNT          309 a          628 c          672 g          355 t
ORIGIN      
        1 cccaagctgg tcctgatgtg gcggctgatc accgaactgc cggagccgga agagccgaga
       61 tacttctgga tgtccacctt cgccggaacc gacgacttcg tacgctttta cgaatgacgg
      121 ggcaggcgcc gaaattcgca atcgcgccgc ggagcgttaa atttgatttt gcggacgagc
      181 caatctgcgc cgccccgccc ccaggcatga tgctgggtct tatggggcta gccatcgtcg
      241 gcggcgaagc cggatggctg ccgggaactc ggtggatatt cttgttgatg ctggccgccg
      301 gcatttttct ggccttcgcc ctgtgtctta caccgcccgt tcttcatgcc tggcatgatc
      361 gagcatggcg tctgcccaaa atcgcttcag cttgtagaac gtggctgggc tcggtccatg
      421 cttacgacgc aagtccgcag tcggcatccc gacctcctgc tccttgatca tcccgataat
      481 ccgcgccccg gcgaaacggc tttttctcat tggtcccttt ttattctctc tcttgaggac
      541 agctttttac cagccgccgc caatcgcgcg gatcagggcg atgcgggcct gatattgggc
      601 tgccctgact ctgagggctt cgcgccggat gctcagcaag gaacgttcgg catcgaggac
      661 ttccagctgc gaggaggagc cttcgcggta gcgcgtctgc gacaggtcgt aggcgcgcaa
      721 ggccgaggcg acggcgtcgg tctcggcgtc ttgctgctgg cgcagataat ccagatcgga
      781 caggctgtcc tcgacctcct gcaaggcggt cagcgtcgtt tggcggtagt tggcgaaggc
      841 ggcgtcgagg ccacctttgg cgtaatcgat ccccgccttg cgcgcgccgc cgtcgaacac
      901 ggcctgcgac agggccgccg ccagcgtaaa agtggtcgag gccggatcga acaggccggt
      961 gattttcggc gaagcgtaac cgccttgcgc cgtcaggctg agcgagggca gccatgccgc
     1021 cttcgccacc ccgacgcgct gggcggcggc gttgaggctg gccctggcgg cgatcaggtc
     1081 gggacggcgg ttcagcacgt cggacggcag gccggcgggc acggcgggga ccgcatccgc
     1141 ccacggctgc cgcgcccagg gttgtgtctc catcgcaaac ccgctggccg gttcgccgac
     1201 cagcacggcc agggcgtgca gggcctgcgc gcgctgctga tcgagcgcgg cggccgccgc
     1261 ctcggtcgag gcgacctcgg ttttcatgcg cgccagatcc agttcggcga cgtcgccctc
     1321 gtcgaaacga tgctgcgtga cggcgaggct ctgacggtag gcgccgagcg tgtcggcgac
     1381 gatggcgcga tcctcatcga gcgcgcgcag ggcgaaatag ctttccgcca cgcgcgactg
     1441 gatcagcagt tgcgcgtctt tcagcagggc ggcgctggcc tgcgcgtcgt catcggcggc
     1501 cctggcggcg cgattcaggc gcccgaacag atcgacctca tagctgagat cggcggtgac
     1561 cgcctgcacg ttggccgcct tgccgggcgg ttgcaagtcg gtcgagcgcg acgggttgta
     1621 gccggcaccg aactgcggcc acaggtccga tcgcgccgaa cctaaaaggg cgcgcgcctg
     1681 ttccagatgc gccgaggcca gagccacatc gttattggcg ctcatggcgc gcgcgatcag
     1741 cccgtccagc gcgtcgtcgt ggaagatcag ccaccactga ccggaagcgg gcgctggcat
     1801 ggcggcggcg gtttgcggac cggcgaaggc ggcgggcgca ataagggtct ttggcggcag
     1861 gggggcctgt ttcaccggcg cggtcgcgca ggcgctaagc aggaccgccg cagaggtgag
     1921 ggtgataaat ttcaacatca gtagctctcc tcgacgcgcg caac
//
LOCUS       DASVLL010000379         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_293488,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000379 DASVLL010000000
VERSION     DASVLL010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293488"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="fahA"
                     /locus_tag="VF402_06275"
     CDS             complement(<1..1027)
                     /gene="fahA"
                     /locus_tag="VF402_06275"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEX7799935.1"
                     /translation="MIDATHDAAARSWVAGADGHPDFPVQNLPFGLFSPPDEAPRPGV
                     AIGDFILDWRALTAAGLAQQTPADLGAVLATPALRRSLRRQVFRLLGDVQYRGAAEPY
                     LHAAADCALHNPVRIGDYTDFYAGIHHAAAAGRLFRPDNPLPPNYKYVPIGYHGRASS
                     ILPSGADITRPSGQSKAPDAEAPSFGPSQRLDFELELGVWIGPGNAMGQPIPIAEAAQ
                     HIGGYGLLNDWSARDLQAWEYQPLGPFLAKSFATTISPWIVTPEALAPFRCAQPPRPE
                     GDPAPLSYLRDEADQAAGALRLDLHVAIRTEAMRAAGLPPHTLSRSAARHLYWTPAQL
                     VAHHASNG"
     gene            complement(1024..>1693)
                     /locus_tag="VF402_06280"
     CDS             complement(1024..>1693)
                     /locus_tag="VF402_06280"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HEX7799936.1"
                     /translation="PELGPIGANGLANARDFETPAAAFEDREAPAELLQKYCGRLWRV
                     MLPHSPLDVVAWHGNYAPYRYDLRRFNTINTVSFDHPDPSIFTVLTSPSDTPGTAHCD
                     FAIFPPRWTVAEHSFRPPWFHRNIMSEFMGLITGAYDAKTDGFLPGGASLHSMMSAHG
                     PDRAAYDAAVAAELAPRKIEDTMAFMWDTRHPLRPTRAALDSPLLQTGYDACWTGFAK
                     ARLP"
BASE COUNT          261 a          528 c          657 g          247 t
ORIGIN      
        1 agccgttgct ggcgtgatgc gccacaagct gcgccggggt ccagtagaga tggcgcgccg
       61 cgctgcggct cagcgtgtgc ggcggcaggc ccgccgcgcg catggcctcg gtgcggatcg
      121 cgacatgaag gtccagcctg agcgcgcccg ccgcctgatc ggcctcgtcc cggagatagg
      181 acaaaggcgc cgggtcgcct tcggggcgcg gcggctgcgc gcaacggaac ggcgccagcg
      241 cctcgggcgt gacgatccag ggcgagatgg tggtggcgaa gctcttggcc aggaacgggc
      301 cgagcggctg gtactcccac gcttgcagat cgcgcgccga ccagtcgttg agcaggccgt
      361 agccgccgat atgctgcgcg gcttccgcga tcgggatcgg ttggcccatc gcgttgccgg
      421 ggccgatcca cacgcccagc tccagctcga aatcgagccg ctgcgacggg ccgaaggacg
      481 gcgcctcggc gtcgggcgcc ttcgactgac cgctgggccg cgtgatatcg gcgccggacg
      541 gcaggatgga cgaggcgcgg ccgtgatagc cgatggggac gtatttataa ttgggcggca
      601 aggggttgtc gggccgaaac agcctgccgg ccgcggcggc gtgatggatg cccgcgtaaa
      661 agtcggtgta gtcgccgatg cggaccggat tatgcagcgc acagtcggcg gcggcgtgca
      721 ggtaaggttc ggcggcgccg cgatattgaa cgtcgccgag cagccggaac acctgccgcc
      781 gcagcgaccg gcgcagggcg ggcgtggcca gcacggcgcc gaggtcggcg ggcgtctgct
      841 gcgccagacc ggcggcggtc agcgcccgcc agtcgaggat gaagtccccg atggcgacgc
      901 cgggacgcgg cgcttcgtcc ggcgggctga acaggccgaa gggcaggttc tgcaccggaa
      961 aatcggggtg gccgtcggcc ccggcgaccc aactgcgcgc ggcggcgtcg tgcgttgcgt
     1021 cgatcatggc agcctcgcct tcgcaaagcc cgtccagcag gcgtcgtaac cggtctgtag
     1081 caggggcgag tccagcgcgg cccgagtcgg gcgcagcgga tggcgcgtat cccacatgaa
     1141 ggccatcgta tcctcgattt tgcgcggcgc caattcggcg gcgacggcgg cgtcataggc
     1201 ggcgcggtcc gggccgtgcg cgctcatcat gctgtgcagg gacgcgccgc cgggcaggaa
     1261 gccgtccgtc ttggcgtcat aggcgccggt gatcaggccc atgaattcgc tcatgatatt
     1321 gcggtggaac cacggcgggc ggaagctgtg ttcggcgacc gtccagcgcg gcgggaagat
     1381 cgcgaaatcg caatgggcgg tccccggcgt gtcggacggc gaggtcagga cggtgaagat
     1441 cgacgggtcg ggatggtcga aactgaccgt gttgatcgtg ttgaaacggc gcaggtcgta
     1501 gcggtagggg gcgtaattgc cgtgccatgc gacgacgtcg agcggcgaat gcggcagcat
     1561 gacgcgccac aaccggccgc aatatttttg cagcaattcc gccggcgcct cgcggtcctc
     1621 gaaggcggcg gcgggcgtct cgaaatcgcg cgcattggcc aggccgttgg cgccgatggg
     1681 gccgagttcg ggc
//
LOCUS       DASVLL010000380         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_293542,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000380 DASVLL010000000
VERSION     DASVLL010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293542"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VF402_06285"
     CDS             complement(<1..154)
                     /locus_tag="VF402_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEX7799937.1"
                     /translation="MCQPTLYKKTYHATHPDMMQNVSNQDLRDKYLLSGLFEPDAVVL
                     NYTHFER"
     gene            711..>2696
                     /locus_tag="VF402_06290"
     CDS             711..>2696
                     /locus_tag="VF402_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799938.1"
                     /translation="MSKHKSSLIRASHVALIVALSCAAASAAQAQDAAQKDRSQPHKT
                     SGGAQAKADAASPVEVIVTGVLAATKAKDANASYSVLTQDDINRFTPISADDMLRDLP
                     GTVVETNDGVARNEVFTRGMTAGTGSNTSGYFWTTILEDGLPVIPFKFGGFQDGYFYR
                     ADISTSRVESVRGGSSATAITTSVGATFNYLSGKIRPGASIQTRFGFEGEDSHLSWKQ
                     VDGYYGWLNKAGDFGLSASGFFRTSTGQVDPGYDLNHGGQFKLNVYKSYDTASGGHGT
                     LTGTYKHLDDTNAELTAFSQPAYGYGDNPENVAGFGRDVDLFLRGGDAQMPDYRDPGY
                     HSLDAQGGFNYKQDAAWLKWDYSTAGRWSFSAAGKAQTSSIRGQGYKANSVVSLSSNL
                     AVRDNMGLNINNLDRTPGYYEFYDAGGTLVAKVANNVAGSQLGVNYHTGAACAKVTSV
                     TTAGRCVVYNALPNGSLDMGGGEVTGSGAPYQTVTLPVGGTDQDLVLTTRIEDSWRQS
                     RDMMFNFGANYTGDTFKVNLGVYAAHSNQQYFQWGAGNGVSAYANGQVENLNVQYVTQ
                     SGTRYQLTNGGGWGGLGAGLFTTAFQYGIINELSPYLGVAWSPTEHWDFNASVKESMY
                     SAKTYSETYDLRNPGAASLTNGGLDGNPLTVYDNVYFV"
BASE COUNT          543 a          837 c          816 g          500 t
ORIGIN      
        1 agcgctcgaa atgggtgtag ttcagcacga ccgcgtccgg ctcgaacagg ccggacagca
       61 ggtacttgtc gcgcagatcc tgattgctga cgttctgcat catgtccgga tgcgtggcgt
      121 ggtaggtctt cttgtaaagc gtgggctggc acatgggggg ctccgttttc atcatatcgg
      181 cccgtccgaa gggacgatgg aaaagccgct tgaatttgat atcgatatca tataaagaac
      241 aaatctcggc ttcgctatgg cttttttcgc cgttccctcg gcgacgccga atcgttcgag
      301 tcgctgcaaa gtgatttgag tttgcttcct tcggaagcct cttctccctg atatccttcg
      361 ccgctgcgtt ttcgcagcgg cgtctttttt aagggcttga attataattg ttattatggt
      421 tcgtcgcaaa tctgtaatat ttgtgcaaca gacccggcgc ttctgacgaa aacgatttgc
      481 gggacgggga aaaatctgat aacgatatca gatatgggcg ctgatcctac acaatgacgc
      541 cccggccatc agggattttt tcaaggaaac cgtcaacctg atatctttgt cgtgagaaaa
      601 ggggttcgca ggggcccgcc gcgcataaag gtcgcgcgca gaaatgatat cgatatcaaa
      661 aacgaacatc cgccgcgaag ccggcggaaa acaggaaaag gggacttaac gtgtcaaaac
      721 ataaatcgag cttgatccgg gcgtcgcatg tcgcgctgat cgtggccctg tcgtgcgccg
      781 ccgcaagcgc cgcgcaggct caggacgccg cgcaaaagga caggtcgcag ccgcacaaga
      841 cgtccggggg cgcgcaggcc aaagcggacg ccgcttcgcc cgtcgaggtg atcgtcaccg
      901 gcgtgctggc cgccaccaag gccaaggacg ccaacgcctc gtattcggtg ctgacgcagg
      961 acgacatcaa ccgtttcacg ccgatcagcg ccgacgacat gctgcgcgac ctgcccggca
     1021 ccgtggtcga aactaatgac ggcgtcgcgc gcaacgaggt gttcacgcgc ggcatgaccg
     1081 caggcaccgg ttcgaacacc tccggctatt tctggaccac gatcctcgaa gacggcctgc
     1141 cggtcatccc gttcaagttc ggcggctttc aggacggcta tttctaccgc gccgacatct
     1201 cgaccagccg cgtcgaatcg gtgcgcggcg gatcgtccgc gaccgcgatc acgacctcgg
     1261 tcggcgcgac gttcaactat ctgtccggca agatccgtcc cggcgcctcg atccagacgc
     1321 gcttcggctt cgagggcgag gattcgcacc tgtcctggaa gcaggtcgac ggctattacg
     1381 gctggctgaa caaggccggc gatttcggcc tcagcgccag cggcttcttc cgcacctcga
     1441 ccgggcaggt cgatccgggc tacgatctca accacggcgg ccagttcaag cttaacgtct
     1501 acaagagcta cgatacggcc agcggcggcc acgggacttt gaccggcacg tacaagcatc
     1561 tggacgacac caacgccgaa ctgaccgcct tctcgcagcc cgcctacggc tatggcgaca
     1621 acccggagaa tgtggcgggg ttcggccgcg acgtggacct gttcctgcgc ggcggcgacg
     1681 cccagatgcc ggattaccgc gatccgggct atcattccct cgatgcgcag ggcggcttca
     1741 actacaagca ggacgccgcg tggctgaagt gggattacag caccgccggc cgctggtcct
     1801 tctcggcggc gggcaaggcg cagacgagca gcatccgcgg tcagggttac aaggccaatt
     1861 cggtcgtgtc gctcagctcc aacctcgcgg tgcgcgacaa tatggggctg aatatcaaca
     1921 atctcgaccg cacgcccggc tattacgaat tctacgacgc cggcgggacg ctggtggcca
     1981 aggtcgccaa caatgtcgcc gggtcgcaac tgggcgtcaa ttaccacacc ggcgcggcct
     2041 gcgccaaggt tacgagcgtg accacggccg gacgctgcgt cgtctataac gcattgccca
     2101 acggcagcct cgacatgggc ggcggcgagg tgacgggcag cggcgcgccg tatcagaccg
     2161 tgaccctgcc cgtgggcggc acggatcagg atctggtgct gaccacgcgc atcgaggatt
     2221 cctggcgtca gtcgcgcgac atgatgttca acttcggcgc caactacacc ggcgacacgt
     2281 tcaaggtgaa cctgggcgtc tatgccgccc attccaacca gcagtacttc cagtggggcg
     2341 cgggcaacgg cgtttccgcc tacgccaacg gtcaggtcga gaacctcaat gtccagtatg
     2401 tgacgcagtc gggaacgcgc tatcagctga ccaacggagg cggctggggc ggactgggcg
     2461 cgggcctgtt cacgaccgcg ttccagtacg ggatcatcaa cgagctctcg ccctatctcg
     2521 gcgtggcctg gtcgccgacc gaacactggg acttcaacgc cagcgtcaag gagtcgatgt
     2581 acagcgccaa gacctattcg gagacctacg acctgcgcaa tccgggcgcc gcgtccctga
     2641 cgaacggcgg cctggacggc aatccgctga ccgtctacga caacgtctat ttcgtc
//
LOCUS       DASVLL010000381         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_509439,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000381 DASVLL010000000
VERSION     DASVLL010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509439"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VF402_06295"
     CDS             complement(<1..828)
                     /locus_tag="VF402_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX7799939.1"
                     /translation="MTRLTGILLAANVLLLGLAGCASLWRGGFDLFPQRAPPPVFHVR
                     GDAILTPQDKPFVARGINLQYGDRPEAAFPALSAIADTGANIVRLELRADTSAHDLRR
                     ALNRAVRLKTPVMVMLWEQDVTCAHDGARLRRYAQDLWMGRWSHVLLDPRYQPYLMLN
                     IANEWGTSADGFGDYMATYRELIRALRGRGYRMPLVIDAADCGQNADSFTAGRGAALE
                     AEDPLRNVIFSVHAYNRPWSDHARIDANLAALKASGLPFLLGEFGSSQLVEDGYSVDH
                     "
     gene            920..2800
                     /gene="dnaG"
                     /locus_tag="VF402_06300"
     CDS             920..2800
                     /gene="dnaG"
                     /locus_tag="VF402_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7799940.1"
                     /translation="MRFDDHFLEELKSRLKPSEVIGKSVRLRRQGREYVGLSPFTKEK
                     TPSFYVNDLKGQFFDFSSGKNGDIISFVQETERLSFNEAVEKLAGLAGMALPAVTPQA
                     IAADMKRQGLGDWMELAHTWFVARLHDRTPAAQNARDYLVRRGLGDADVAAFGLGYAP
                     RDHSALKDALVQKGAKVSELVECGLLIEVEGKAPYDRFRDRLIFPIHDQRGRIVSFGG
                     RALSPDEKAKYLNGPETPLFHKGHMLFGLPGAVKLLGAETGGDLVVVEGYMDVLACQR
                     ARVAAVAPLGTALTEEQIELLWRRHPEPVLCFDGDAAGQRAAYRALDRALPKLRPGRS
                     FRVCLLTGGKDPDEILREKGALALREALKDTKPFVEVLFQRELELEPLDTPERKAGLK
                     KRLRQLAAQIEDKDLGEAYREDLLGRYTQMTAPQRSAPAFADGPAPRREWRPGLRRGE
                     PFLPPVLPESRIAAHNLSSALDPTAAALAQGLIDRPDWMDDQVEALEAYGFGDPALEP
                     LARALVALSFAHSGLDSETLSRHLHRQGLGALLGEIQKAALKSGAPFLTEPSQADAHA
                     RWLMAFNAVSRLTALDRALNSARDQVGTEFFSRLKLERDGLRRSLRSGHIWRDSETKA
                     SG"
     gene            2919..>3523
                     /locus_tag="VF402_06305"
     CDS             2919..>3523
                     /locus_tag="VF402_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEX7799941.1"
                     /translation="MSTATEKPEAETSSDGPLLDLTDSAVKKFIKAAKARGYVTMDEL
                     NKVLPSEEFTSEQIEDTLAMLTEMGVNVIESEEDAAEGGEIAVREEAGAVATTEKTSS
                     YDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRDTMIRGLCESALTFEAIMVWR
                     EELASGRILLREIIDLEGTYGGGPNDGPPAGALNEGEDEEEA"
BASE COUNT          610 a         1143 c         1211 g          559 t
ORIGIN      
        1 gtgatcgacg ctgtagccgt cctcgaccaa ctggctggag ccgaactcgc cgagcaggaa
       61 gggcaggccg ctggccttga gcgcggcgag attggcgtcg atcctggcgt gatcgctcca
      121 cggcctgtta taggcgtgga cgctgaagat cacgttgcgc agcgggtctt cggcctccag
      181 cgcggcgccg cgcccggcgg tgaagctgtc cgcgttctgg ccgcagtcgg cggcgtcgat
      241 caccagcggc atgcggtagc cgcgcccgcg cagggcgcgg atcagttcgc gataggtcgc
      301 catgtagtcg ccgaacccgt cggcggacgt cccccattcg ttggcgatgt tgagcatcag
      361 gtacggctgg tacctcggat cgagcagcac gtggctccag cggcccatcc acaggtcttg
      421 cgcataccgg cgcagccgcg cgccgtcgtg cgcgcaggtc acgtcctgct cccacagcat
      481 gaccatgacc ggcgttttca gcctgacggc ccggttcagc gcgcggcgca ggtcgtgcgc
      541 cgaggtatcg gcgcgcaact ccagccggac gatgttggcg cccgtgtccg caatcgcgct
      601 cagcgccgga aaggcggcct cgggccggtc gccgtattgc agattgatcc cgcgcgcgac
      661 gaagggctta tcctgcggcg tcaggatggc gtcgccgcgc acgtgaaaga ccggcggcgg
      721 cgcgcgctgc gggaacaggt cgaagccgcc gcgccacagg ctggcgcagc ccgccagccc
      781 cagcaacagg acgttggcgg ccagcaggat ccccgtcaga cgtgtcacgc gcaatgccat
      841 gcgcccagcc tgacaaaaaa gcctgaagga ggcttaaacg aatcttcgca acaaagcgtg
      901 tttcgcctat agtcgcgccg tgcgtttcga cgatcatttc ctggaagaac tcaaatctcg
      961 cctgaaaccc tctgaggtca tcggcaagag cgtccggctg cgccgtcagg ggcgcgaata
     1021 tgtcggcctg tcgccgttca ccaaggaaaa gacgccgtcc ttctacgtca acgacctgaa
     1081 gggccagttc ttcgatttct cgtccggcaa gaatggcgac atcatctcct tcgtgcagga
     1141 gacggagcgg ctgagcttca acgaggcggt cgagaagctg gccggtctgg cgggcatggc
     1201 cctgcccgcc gtcacgccgc aggccatcgc cgccgacatg aagcggcagg gtctgggcga
     1261 ctggatggag ttggcgcaca catggttcgt cgcgcggctg cacgaccgga cgcccgccgc
     1321 gcagaacgcc cgcgactatc tggtcaggcg cggcctcggc gacgccgacg tcgccgcctt
     1381 cggcctcggc tacgcgccgc gcgaccacag cgcgctgaaa gacgcgctgg tgcagaaggg
     1441 cgcgaaggtg tccgaactgg tcgaatgcgg cctgctgatc gaggtcgagg gcaaggcgcc
     1501 ctacgaccgc ttccgcgacc ggctgatctt tcccatccac gatcagcgcg ggcgcatcgt
     1561 ctcgttcggc gggcgcgcgc tcagcccgga cgagaaggcg aaatatctca acggccccga
     1621 aacgccgctg tttcacaagg ggcatatgct gttcggcctg cccggcgcgg tgaagctgct
     1681 gggcgccgag acgggcggcg atctcgtcgt ggtcgagggc tatatggacg tgctggcgtg
     1741 ccagcgcgcc agggtcgccg ccgtcgcgcc gctgggcacc gccctgaccg aggagcagat
     1801 cgagcttctg tggcgccgcc atcctgagcc ggtgctgtgc ttcgacggcg acgcggcggg
     1861 ccagagggcg gcctatcgcg cgctcgaccg cgccctgccg aagctcaggc cggggcgcag
     1921 tttccgcgtc tgcctgctga ccggcggcaa agacccggac gaaatcctgc gcgaaaaggg
     1981 cgccctggcc ctgcgcgagg cgctcaagga caccaagccg ttcgtcgagg tgctgttcca
     2041 gcgcgagctt gagcttgagc cgctcgacac ccccgaacgc aaggcggggc taaagaagcg
     2101 cctgcggcaa ctggcggcgc agatcgagga caaggatctg ggcgaagcct atcgcgagga
     2161 tctgctgggc cgctacacgc agatgactgc gccgcagcgt tccgcgcccg ccttcgccga
     2221 cggcccggcg ccgcgccggg aatggcgtcc cggcctgcgg cgcggcgagc cgttcctgcc
     2281 gccggtgctg ccggaatcga ggatcgccgc gcacaacctg tcgtccgcgc tcgatccgac
     2341 cgccgccgcg ctggcgcagg ggctgatcga ccggcccgac tggatggacg atcaggtcga
     2401 ggcgctggag gcctacggct tcggcgatcc ggcgctggag ccgctggcgc gcgcgctggt
     2461 ggcgctcagt ttcgcgcatt cggggcttga cagcgaaacc ttgagtcgcc atctgcacag
     2521 gcagggactt ggagcattgt taggcgagat tcaaaaggcg gccctgaaat ccggcgcgcc
     2581 cttcctgacg gagccatctc aagccgatgc ccatgcccgc tggcttatgg cgttcaacgc
     2641 ggtttcgcga ttgacggcct tggaccgcgc cttgaattcc gcccgcgatc aggtgggcac
     2701 ggaatttttt tcgcggctca agctcgaacg cgacgggctg cggcggtcct tgagaagcgg
     2761 acatatctgg cgggactccg agacgaaggc atcgggctga tgcgttaagg ctaagacttc
     2821 gttgaggaat atcctgaata tacacttatg cgaccttacg ccgtcacggc gggcgcggag
     2881 acgatgatga ttttgacagc cttttcgagt gtggcagaat gagcacagcg acggaaaaac
     2941 ctgaagccga gacgtcctcc gacggcccgt tgctcgacct gaccgattcc gccgtcaaga
     3001 agttcatcaa ggccgccaag gcgcgcggct atgtgacgat ggacgagctg aacaaggttc
     3061 tgccctcgga agaattcacc tcggagcaga tcgaggacac gctggccatg ctcaccgaga
     3121 tgggcgtgaa cgtcatcgag tccgaggaag acgccgccga gggcggcgag atcgcggtgc
     3181 gcgaggaagc gggcgcggtc gcgacgacgg aaaagacctc gtcttacgac cgcaccgacg
     3241 atccggtgcg catgtacctg cgcgaaatgg gctcggtcga gcttctgtcg cgcgagggcg
     3301 aaatcgccat cgccaagcgc atcgaggcgg gccgcgacac catgatccgc ggcctgtgcg
     3361 aatcggccct gaccttcgag gccatcatgg tgtggcgcga ggagttggct tccggccgca
     3421 tccttttgcg cgagatcatc gatctggaag gcacctatgg cggcggtccg aacgacggcc
     3481 cgcccgccgg cgcgctcaat gagggcgagg acgaggagga ggc
//
LOCUS       DASVLL010000382         4451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_455107,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000382 DASVLL010000000
VERSION     DASVLL010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455107"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VF402_06310"
     CDS             <1..200
                     /locus_tag="VF402_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799942.1"
                     /translation="RYNGPDANWAEPDLRAARESLGRLIATEDLRRRLGGRARQDMIA
                     LNESWQDQAAWLPPASESLAF"
     gene            312..1433
                     /locus_tag="VF402_06315"
     CDS             312..1433
                     /locus_tag="VF402_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7799943.1"
                     /translation="MSAVTLVACSHKDDSKAGARGPSPVGYIVVHTQPVQVTQELAGR
                     TSAWLVADVRPQVTGIVKSRLFTEGGAVRAGQSLYQIDPATYQAAYDSALAAEQQAQA
                     SYDLAQTNAQRAQQLITVKAISQADFDSAQAAGKQAAATLSAQKANVETARINLNYTK
                     VLSPISGRIGKSSVTAGALVTANQTTALATVQDLSKIYVDITQSTADLLKLKRAIATG
                     QLGAPDHADVTLTLDDGTTYNQTGRIEFSDVTVDPSTGSVTLRAVFPNPDGVLLPGMF
                     VRARIVNGTAPNSVLIPQGAVILDPKGGATVLIAGGDGKAQSRKVGLGQMIGNQWQIT
                     SGLNAGDRVITEGAMKLKAGAAIKAEPVRAAPAPAGSAE"
     gene            1440..>4451
                     /locus_tag="VF402_06320"
     CDS             1440..>4451
                     /locus_tag="VF402_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7799944.1"
                     /translation="MNISRFFIDRPIFAWVIAIVIMLAGLLSIFTLPIEQYPRIALPQ
                     VTVNATYTGASAKVVEDSVTQVIEQSLTGLDHLKYMSSTSSAGSASVTLVFDAGTDPD
                     FAQVQVQNQVQSALRRLPQDVQQQGLRVNKASGTALMSIAFYTDDDSMSNADISDWVA
                     SNLNDPISRLNGVGDTRVFGSQYAMRIWLNADQLAAYSLTPSDITSAIEAQNTQVSAG
                     TIGASPSTPDTALTATITAQSQLQTPAQFENIIVKNNPDGSPVLLKDVARVELGPQNY
                     GFAARMNGHIASGMQVSLAIGANALKTADEVKALLNRMEPSFPAGLKYAIPNDSTQFV
                     KLSIEDVVKTLVEAVVLVFVVMFIFLQNWRATLIPAIAVPVVLLGAFGILAAFGYSIN
                     TLTMFGLVLAIGLLVDDAIVVVENVERVMTEEGLDPKEATRRSMDEITGALIGVASVL
                     AAMFVPMAFFGGTQGIIYRQFSVTLVSAMALSVIVALILTPPLCATLLHRPKPGEGIP
                     PAEEGDDDVKVHGVLSAFNRGFQVFAHRYQMSVRGIIRKPGLFLGVYGLIVAALVALA
                     MTLPTSFLPDEDQGILTTIVQLPAGSTSPQTQKVLEQVQNVYMKDKAVQYVFSVAGFS
                     FGGSGENQGMVIARMKDFKYRHAADMKVQAVQARARKAFARMHNATIIPTIPPPVREL
                     GNASGFDLELKDTSGAGHTQLANARDQLVALANRNPVLTGVRANVQDDTPELHIDIDN
                     TKAGALGVKVDDVNAMLATALGGTYVNDFLDRGRVKRVYVEGDAQFRARPEDINRWFV
                     RNSAGQMVPFSAFGSADWSFGPPQLQRYNGFGSMEIQGAAAQGQSNGKAMQIMESLVK
                     QLPGNWGFEWTGLSLQEQESGQQAPALYALSLLVVFLLLAALYESWSIPFAVIFVVPL
                     GIFGALVGTWLRGLDNDIYFQVGLLATMGLSAKNAILIVEFAKILHEEGRGLIAATLE
                     AVRIRLRPIIMTSLAFTFGILPLALANSAGSS"
BASE COUNT          760 a         1480 c         1461 g          750 t
ORIGIN      
        1 agagatacaa cggccccgac gccaattggg cagagcccga cctgcgcgcc gcccgcgaaa
       61 gccttggccg gttgatcgca accgaagacc tgcgtcgtcg gctgggcgga cgcgcccggc
      121 aggatatgat cgctttgaac gagtcgtggc aggatcaggc ggcatggctg ccgcccgcgt
      181 ctgaatcatt agccttctaa atattttttg ccctgcaccg ctggaatgtc gcatcccctg
      241 cgtatatgtg ggcaacttcc taagagggcc tgatgttgaa agcgaaattt gtgaccgccc
      301 cgcttctggt tctgagcgcc gtcacgctgg ttgcctgctc ccataaggac gattccaagg
      361 ccggggcgag gggcccctcg ccggtcggtt atatcgtcgt tcatactcag ccggtgcagg
      421 tgacgcagga gttggcgggg cggacttcgg cctggctggt ggcggatgtg cggccgcagg
      481 tgacggggat cgtcaagtcg cgtctgttca ccgagggcgg cgcggtcagg gcggggcagt
      541 cgctttatca gatcgatccg gccacctatc aggccgctta tgacagcgcg ctggccgccg
      601 agcagcaggc gcaggcgtct tacgatctgg cgcaaaccaa tgcgcagcgc gcgcagcaac
      661 tgatcacggt caaggcgatc agccaggccg atttcgactc cgcgcaggcc gccgggaagc
      721 aggccgcggc cacgctgtcg gcgcagaagg ccaatgtcga gacggcgcgc atcaacctga
      781 actacaccaa ggtgctgtcg ccgatttcgg gccgcatcgg caagtcgtcg gtgacggcgg
      841 gcgcgctggt gaccgccaac cagacgacgg cgctggccac ggtgcaggat ctgtcgaaaa
      901 tctatgtcga catcacgcaa tcgaccgccg acctcctgaa gctgaagcgc gccatcgcca
      961 cgggccagtt gggcgcgccc gaccatgccg acgtcaccct gacgctggat gacggcacga
     1021 cctacaacca gaccggccgc atcgaatttt ccgacgtgac ggtcgatccg tcgaccggct
     1081 cggtgacgct gcgcgcggtg ttcccgaacc cggacggcgt tttgctgccc ggcatgttcg
     1141 tgcgggcgcg catcgtcaac ggcacggcgc cgaacagcgt gctgatcccg cagggcgcgg
     1201 tgatcctgga cccgaagggc ggcgccacgg tgctgatcgc gggcggcgac ggcaaggcgc
     1261 agagccgcaa ggtcggctta gggcagatga tcggcaacca gtggcagatc acctccggcc
     1321 tgaacgcggg cgacagggtg atcaccgagg gcgccatgaa gctcaaggcc ggcgcggcga
     1381 tcaaggccga accggtcagg gcggcgcccg cccccgccgg aagcgcggag tagcccgtcg
     1441 tgaacatttc gcgtttcttc atcgaccggc ccatcttcgc gtgggtcatc gccatcgtca
     1501 tcatgctggc cgggctgttg tcgatcttca ccctgccgat cgagcagtat ccgcgcatcg
     1561 ccctgccgca ggtcaccgtc aacgccacct acaccggcgc ctcggccaag gtggtcgagg
     1621 attcggtcac gcaggtgatc gaacagagcc tgaccggcct cgatcacctc aaatacatgt
     1681 cctcgacctc gtcggcgggt tcggccagcg tcacgctggt cttcgacgcg ggcaccgacc
     1741 ccgacttcgc gcaggtgcag gtgcagaacc aggtgcagtc cgccctgcgc cgcctgccgc
     1801 aggacgtgca gcagcagggc ctgcgcgtca acaaggcgtc gggcacggcg ctgatgtcga
     1861 tcgccttcta taccgacgac gacagcatga gcaacgccga catctcggac tgggtggcgt
     1921 cgaacctgaa cgatccgatc tcgcgcctga acggcgtcgg cgacacgcgc gtgttcggct
     1981 cgcaatacgc gatgcgcatc tggctcaacg ccgatcaact ggccgcctat tcgctgacgc
     2041 cttccgacat cacctccgcc atcgaggcgc agaacacgca ggtgtccgcc ggcaccatcg
     2101 gcgccagccc ctcgacgccc gacaccgcgc tgaccgcgac catcaccgcg caatcgcagc
     2161 tgcagacccc ggcgcagttc gagaacatca tcgtcaagaa caatcccgac ggctcgcccg
     2221 tgctgctgaa ggacgtggcg cgcgtcgagc ttggcccgca gaattacggc ttcgcggcgc
     2281 gcatgaacgg ccatatcgcg tcggggatgc aggtgtcgct ggccatcggc gccaacgcgc
     2341 tgaaaaccgc cgacgaggtc aaggccctgc tcaaccgcat ggagcccagc ttcccggcgg
     2401 gcctgaaata cgccattccg aacgactcga cgcagttcgt gaagctgtcg atcgaggacg
     2461 tggtcaagac gctggtcgag gccgtcgtgc tggtcttcgt ggtgatgttc atcttcctgc
     2521 aaaactggcg cgcgaccctg atcccggcca tcgccgtgcc ggtcgtgctt ctgggggcgt
     2581 tcgggattct ggccgccttc ggctattcga tcaacaccct gacgatgttc ggtctcgtgc
     2641 tggccatcgg gcttctggtc gacgacgcca tcgtggtggt cgagaacgtc gagcgcgtga
     2701 tgaccgagga agggctggac cccaaggagg cgacccggcg ctcgatggac gagatcaccg
     2761 gcgcgctgat cggcgtcgcc tcggtgctgg cggccatgtt cgtgccgatg gcctttttcg
     2821 gcggcacgca gggcatcatc taccgccagt tctcggtgac cctggtgtcg gcgatggcgc
     2881 tgtcggtgat cgtggcgctg atcctgaccc cgcctttgtg cgcgaccctg ctgcacaggc
     2941 ccaagcccgg cgagggcatc ccgcccgccg aggaaggcga cgacgacgtc aaggtccacg
     3001 gcgtgctgtc cgccttcaac cgcggctttc aggtcttcgc ccaccgctat cagatgtcgg
     3061 tgcgcggcat catccgcaag cccggcctgt ttctgggcgt gtacgggctg atcgtggcgg
     3121 cgctggtcgc gctggccatg accctgccga cctcgttcct gccggacgag gatcagggca
     3181 tcctgaccac catcgtgcaa ctgcccgccg gctcgacctc gccccagacg cagaaggtgc
     3241 tggagcaggt gcagaacgtc tatatgaagg acaaggcggt ccagtacgtg ttctcggtcg
     3301 cgggcttcag cttcggcggc tccggtgaaa atcagggcat ggtcatcgcg cgcatgaagg
     3361 acttcaaata ccgccacgcc gccgacatga aggtgcaggc cgtgcaggcg cgcgcgcgca
     3421 aggcgttcgc caggatgcac aacgcgacca tcatcccgac cattccgccg cccgtgcgcg
     3481 agctgggcaa cgcatccggc ttcgatctgg agctgaagga caccagcggc gccggacaca
     3541 cgcaactggc caacgcgcgc gatcagctgg tcgcgctggc caaccgcaat ccggtgctga
     3601 ccggcgtgcg cgccaacgtg caggacgaca cgccggaact gcatatcgac atcgacaaca
     3661 ccaaggcggg cgcgctgggc gtgaaggtcg acgacgtcaa cgccatgctg gcgacggcgc
     3721 tgggcggcac ctatgtcaac gacttcctcg accgcggccg cgtcaagcgc gtctatgtcg
     3781 agggcgacgc gcagttccgc gcccgcccgg aggacatcaa ccgctggttc gtgcgcaatt
     3841 cggcgggcca gatggtgccc ttctcggcct tcggctcggc ggactggagc ttcggcccgc
     3901 cgcagttgca gcgctataac ggcttcggct cgatggagat tcaaggcgcc gccgcgcagg
     3961 gccagtcgaa cggcaaggcg atgcagatca tggagagcct cgtgaagcaa ctgcccggca
     4021 actggggctt cgaatggacc ggcctttccc tgcaggaaca ggaatcgggc cagcaggcgc
     4081 ccgcgctcta cgcgctgtcg ctgctggtgg tgttcctgct gctggcggcg ctgtacgaaa
     4141 gctggtcgat cccgttcgcg gtcatcttcg tggtgccgct ggggattttc ggcgcgctgg
     4201 tcggcacctg gctgcgcggg ctggacaacg acatctattt ccaggtcggg cttctggcca
     4261 ccatggggct gtcggccaag aacgccatcc tgatcgtcga attcgccaag atcctgcacg
     4321 aggaaggccg ggggctgatc gccgccacgc tggaggccgt gcgcatccgg ctgcggccga
     4381 tcatcatgac ctcgctggcc ttcaccttcg gcatcctgcc gctggccctg gccaattcgg
     4441 cgggatcgtc g
//
LOCUS       DASVLL010000383         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_399759,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000383 DASVLL010000000
VERSION     DASVLL010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399759"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VF402_06325"
     CDS             <1..572
                     /locus_tag="VF402_06325"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX7799945.1"
                     /translation="HGVTIWDEWADANGDLGPVYGKQWRSWAAPDGRTIDQMAQLVEN
                     LKTNPYSRRHVISAWNPAEVEDMALPPCHCLFQFFVADGRLSCQLYQRSADVFLGVPF
                     NIASYALLTHMVAQATGLEAGDFVHTFGDVHLYNNHIEQAKTQLARAPYAFPTLKLAP
                     KTDLFGFDYADIAIENYQSHGALKAPVAV"
     gene            569..1084
                     /locus_tag="VF402_06330"
     CDS             569..1084
                     /locus_tag="VF402_06330"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX7799946.1"
                     /translation="MSRPRLALVVAMSENGVIGRDGGLPWHIRSDLKNFKAITQFKPV
                     IMGSTTWESLPRKPLPGRLNLVLSRDPKFEAEGGVVCSTLMEALDIAREHAGDDGADE
                     ICVIGGANVYEQTLPKADRLYVTHVHAEIEGDTRFTAIDPHIWKVVREDRFEKGEGDD
                     YAFTLKVYERN"
     gene            complement(1069..2181)
                     /locus_tag="VF402_06335"
     CDS             complement(1069..2181)
                     /locus_tag="VF402_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX7799947.1"
                     /translation="MSDISADQVLAVLNAIVDPATGKGLADAGMVKALIARPERVGFM
                     LEVPQDRVTQYGPVRLAAEKALKAAFPQAAKTQVVLTAEMAAPEAPKGPSARLSERAV
                     ADGRPKAPVANARPNHVRRVIVVGSGKGGVGKSSVSLALAIGLSQLGLRVGLLDADIY
                     GPSVPVMLGLSQPPSFGADKLMVPPEAHGLKVNSVGFLVDPGQAMIWRGPMASQALTQ
                     LLTQTRWGTAEEPLDVLVVDLPPGTGDVQLTLTQKTLIDGAVVVSTPQEMALADARRA
                     VTLFGKTGVRVLGVVENMAFLALPDGAEMEVFGRGGAKAMAQALDAPFLGEVPLDPAL
                     RAGCDTGRPVTAVQPDGDLARRFRAIAQAIMDQLRS"
     gene            2264..>2766
                     /locus_tag="VF402_06340"
     CDS             2264..>2766
                     /locus_tag="VF402_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEX7799948.1"
                     /translation="MTPADAPLYLLAGPTASGKSAKALDWAGRTGGVILNADSMQLYR
                     DVPILTARPSRADEARAPHALYGLLDAQTLWSAGDWLRAARPFLAEALNGGPPVCVVG
                     GTGLYFNALIHGLAEVPEIADAAREQARGDYERLGEAAFRDRLRQCDPQAEARILPND
                     RQRLTRA"
BASE COUNT          464 a         1001 c          879 g          422 t
ORIGIN      
        1 accacggcgt caccatctgg gacgaatggg ccgacgccaa cggcgacctc ggccccgttt
       61 acggcaaaca atggcgctca tgggcggccc cggacggacg gacgatcgac cagatggcgc
      121 aactggtcga gaacctgaaa accaacccct attcgcgccg ccatgtgatt tcggcctgga
      181 acccggcgga ggtcgaggac atggccctcc cgccgtgcca ttgcctgttc cagttcttcg
      241 tcgccgacgg caggctgtca tgccagcttt accagcgctc ggccgacgtc tttctcggcg
      301 tgccgttcaa catcgcctcc tacgcgcttc tgacccatat ggtggcccag gcgacgggtc
      361 tggaggccgg cgacttcgtg catacgttcg gcgacgtgca tctctacaac aaccatatcg
      421 agcaggcgaa gacccagctt gcgcgcgcgc cctatgcgtt cccgacgctg aaactggcgc
      481 cgaaaaccga cctgttcggc ttcgactacg ccgacatcgc gatcgagaac taccagtcgc
      541 acggcgcctt gaaagcgccg gtggcggtat gagccgcccc aggctggcgc tggtcgtcgc
      601 catgagtgaa aacggcgtga tcggccgcga cggcggcctg ccgtggcata tccgctccga
      661 cctgaagaac ttcaaggcga tcacccaatt caagccggtg atcatgggct cgaccacctg
      721 ggaatcgctg ccgcgcaagc cgctgccggg gcggctcaac ctcgtcctgt cgcgcgatcc
      781 gaagttcgag gccgaaggcg gcgtcgtctg ctcgaccctg atggaggcgc tcgacatcgc
      841 gcgcgaacac gccggggacg acggggctga cgaaatctgc gtcatcggcg gggccaatgt
      901 ctatgagcag accctgccga aggccgaccg gctctacgtc acccacgtcc atgccgagat
      961 cgaaggcgat acccgtttca cggccatcga cccgcatatc tggaaagtcg tgcgcgagga
     1021 caggtttgaa aaaggcgaag gcgacgacta cgcctttacg ctgaaggttt acgaacgcaa
     1081 ctgatccatg atcgcctgcg cgatggcgcg gaagcgtcgc gccagatcgc cgtcgggctg
     1141 aaccgccgtc accggccgcc ccgtatcgca cccggcgcgc agggccggat cgagcggaac
     1201 ctcgccgagg aacggagcgt ccagcgcctg cgccatcgcc ttggcgccgc cgcgtccgaa
     1261 cacctccatc tctgcgccgt cgggcaaggc cagaaacgcc atgttctcga ccacgcccag
     1321 cacgcgcacg ccggtcttgc cgaacagggt caccgcgcgg cgcgcgtcgg ccagcgccat
     1381 ctcctgcggc gtcgacacca ccaccgcgcc gtcgatcagc gtcttctgcg tcagggtcag
     1441 ttgcacgtcg cccgtgcccg gcggcagatc gaccaccagc acgtcgagcg gttcctccgc
     1501 cgtgccccag cgcgtctggg tcagcaactg cgtcagggcc tgcgacgcca tcggcccgcg
     1561 ccagatcatc gcctgccccg gatcgaccag gaagccgacc gaattgacct tcagcccgtg
     1621 cgcttccggc gggaccatca gcttgtcggc gccgaacgac ggcggctgcg acagccccag
     1681 catgaccggg accgacgggc cgtagatatc ggcgtccagc aacccgacgc gcaggcccag
     1741 ttgcgacagg ccgatcgcca gcgccagcga cacgctcgac ttgccgaccc cgcccttgcc
     1801 ggagccgacc acgatgacgc gccggacatg gttgggccgc gcgttggcca ccggcgcctt
     1861 gggcctgccg tccgcgaccg cgcgctccga caggcgcgcg gacgggcctt tgggcgcttc
     1921 cggcgcggcc atttcggcgg tcagcaccac ctgcgtcttg gcggcctgcg gaaacgccgc
     1981 cttcagcgcc ttttccgccg ccagccgcac ggggccgtat tgcgtcaccc ggtcctgcgg
     2041 aacctccagc atgaagccga cgcgctccgg gcgcgcgatc agcgccttga ccatgcccgc
     2101 atcggccaga cccttgccgg tcgccggatc gacaatcgcg ttcaggacgg ccagaacctg
     2161 atcggcgctg atatcggaca tttgagcagg catggacgaa ccttcacgca atctttaaaa
     2221 cgcgcttcac ttaggccctt tatccgcgtg tttaaagacc aaaatgaccc ccgccgacgc
     2281 tcccctctat ctgctggccg gaccgaccgc ttcgggcaag tcggcgaagg cgctcgactg
     2341 ggccgggcgc accggcggcg tgatcctcaa cgccgattcg atgcagttgt accgcgacgt
     2401 tccgatcctg accgcgcgcc cgtcgcgggc ggatgaggcg cgggcgccgc atgcgctgta
     2461 cggccttctg gacgcgcaaa ccctgtggtc cgccggggac tggctgcgcg cggcgcggcc
     2521 cttcctcgcc gaggcgctga acggcgggcc gcctgtctgc gtcgtcggcg gcacggggct
     2581 ttatttcaac gcgctgatcc acggtctggc cgaagtcccg gagatcgcgg acgccgcccg
     2641 cgaacaggcg cgcggcgact atgagcggct gggcgaggcg gccttccgcg accgcctgcg
     2701 gcaatgcgac ccgcaggccg aggcgcgcat cctgccgaac gaccgccagc gcctgacgcg
     2761 ggccta
//
LOCUS       DASVLL010000384         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_455210,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000384 DASVLL010000000
VERSION     DASVLL010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455210"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VF402_06345"
     CDS             <1..224
                     /locus_tag="VF402_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7799949.1"
                     /translation="VTYAAYPDYVKSAPMEPQIAPNGPIRDALDYRHRFPDGRIGSDS
                     SLATVADGEQIIAAAVKGLLDDVARFEAE"
     gene            complement(225..1043)
                     /locus_tag="VF402_06350"
     CDS             complement(225..1043)
                     /locus_tag="VF402_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7799950.1"
                     /translation="MADDNALGGFLRDRRARLDPAAFGIPLARRRTPGLRREEVAQRA
                     NVSATWYTWLEQGRGGAASADVLDRIARALMLTEAEREHLFLLGLGRPPETRYRAADS
                     ISPRLQGVLDALPYSAAYIKTPMWDIVAWNRAASKVLTDYGALPPERRNILRLMFGAK
                     HVRAKQADWRRITRFVVATFRADVVRAGAVAEVEGLVAELCAMSAEFAALWRENNVQS
                     GYGDHPKRIEHPTAGWIALDYCAFAVDGRPDLQLVVYNPATPEDMAKVKALVEA"
     gene            1139..2032
                     /locus_tag="VF402_06355"
     CDS             1139..2032
                     /locus_tag="VF402_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7799951.1"
                     /translation="MRVFVTGASGWVGSVVTQELLRHGHSVLGLARSDASAAAVTAAG
                     ADVLRGDVRDTDSLIAGAQSADGVIHTAFIHDFSKFVENCEIDRRAVTAMAGVLADTG
                     KPFVVTSGTGVVSSADRTSEDVRVADVRHSFPRIASEQAAEAAAARGVNVSVVRLSPS
                     VHGAGDHGFVPRLIQLARETGKAAYVGAGDNVWPGVHRLDAARLYRLALERNAQDARY
                     HAAENRGTPFRAIAETIGRRLGLPVASLTPDAAPAHFGWLSPFAMLDNPTSSDWTRAT
                     LGWAPTQPDLPGDLDSAAYFA"
     gene            complement(2038..>2424)
                     /locus_tag="VF402_06360"
     CDS             complement(2038..>2424)
                     /locus_tag="VF402_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7799952.1"
                     /translation="LGRNAHAALITRGFAEMTRLAIAMGAQLATMAGLCGMGDLVLTC
                     SSPQSRNMSVGLALGQGQTLEAALAGKVSVAEGVQSAPAVCDLGRKNGVDLPLCNAVN
                     AVLSGGMSVDQAIDALLSRPLKAERV"
BASE COUNT          369 a          881 c          809 g          365 t
ORIGIN      
        1 ccgtcaccta cgccgcctat cccgattacg tgaaatccgc gccgatggag ccgcaaatcg
       61 cgcccaacgg cccgatccgc gacgcgctcg actaccgcca ccgcttcccc gacggccgca
      121 tcggctcgga ttcgtcgcta gccacggtcg cggacggcga acagatcatc gccgccgccg
      181 tcaagggcct gctcgacgac gtggccaggt tcgaggcgga gtagtcaggc ctcgaccagc
      241 gccttcacct tcgccatgtc ttccggcgtg gcgggattat agacgaccag ttgcaggtcg
      301 gggcggccgt cgaccgcgaa ggcgcaataa tcgagcgcga tccagcccgc cgtcggatgc
      361 tcgatgcgct tggggtgatc gccgtagccg ctctggacat tgttttcgcg ccacagcgcg
      421 gcgaattccg cgctcatcgc gcacagttcc gcgaccagcc cctcgacctc ggcgaccgcc
      481 ccggcgcgca ccacgtcggc gcgaaaggtc gcgaccacga agcgcgtgat ccggcgccag
      541 tcggcctgtt tggcgcgcac atgcttcgcc ccgaacatca gccgcaggat attgcgccgc
      601 tccggcggca gggcgccgta atcggtcagc accttcgacg ccgcccggtt ccaggcgacg
      661 atgtcccaca tcggcgtctt gatataggcg gcgctgtacg gcagcgcgtc gagcacgccc
      721 tgcaaacgcg ggctgatgct gtcggcggcg cggtagcgtg tctccggcgg acgccccaga
      781 cccagcagga acagatgctc gcgctccgcc tcggtcagca tcagggcgcg cgcgatccgg
      841 tccagcacgt cggccgaggc cgccccgccg cgcccctgct ccagccaggt gtaccaggtg
      901 gcgctgacat tggcgcgctg cgccacctcc tcgcggcgca ggcccggcgt gcggcggcgc
      961 gccaacggaa tgccgaacgc cgccggatcg agccgggcgc gccggtccct cagaaagccg
     1021 ccaagcgcgt tgtcgtccgc catgctgtta agatttatac ctgtataaat tcacgacttt
     1081 tacccggata agatacgcgc catatcacct ctcgtccagt tttataagga gagaacacat
     1141 gcgggttttt gtaacgggcg cttcgggctg ggtcgggtcg gtggtcacgc aggagttgct
     1201 gcgccacggc cacagcgttc tggggctggc gcggtcggac gcttcggcgg cggcggtgac
     1261 ggcggcgggc gcggacgtgc tgcgcggcga cgtgcgcgac acggacagcc tgatcgcggg
     1321 cgcgcaatcg gcggacggcg tgatccacac cgccttcatc cacgacttct cgaaattcgt
     1381 ggaaaactgc gagatcgacc ggcgcgccgt cacggcgatg gccggggttc tggccgatac
     1441 gggcaagccc ttcgtcgtca cctccggcac gggcgtcgtc tcctcggcgg acaggaccag
     1501 cgaggacgtc cgcgtcgccg atgtgcgcca ttccttcccg cgcatcgcct cggaacaggc
     1561 cgccgaagcc gccgccgccc gcggcgtcaa cgtgtcggtc gtgcgcctgt cgccgtcggt
     1621 gcatggcgcg ggcgaccacg gcttcgtgcc gaggctgatc cagctggcgc gcgaaacggg
     1681 caaggcggcc tatgtcggcg cgggcgacaa tgtctggccg ggcgtgcatc ggctggacgc
     1741 ggcgcgcctg taccggctgg cgctggagcg caacgcgcaa gacgcgcgct atcacgccgc
     1801 cgagaaccgg ggaacgccct tccgcgccat cgccgaaacc atcggcaggc gcctcggcct
     1861 ccccgtcgcg agcctgacgc cggacgcggc gccggcgcat ttcggctggt tgtcgccgtt
     1921 cgccatgctg gacaatccga cgtcgagcga ctggacgcgc gccacgctcg gctgggcgcc
     1981 gacgcagccc gacctgcccg gcgatctcga cagcgccgcc tatttcgcct gaaatcccta
     2041 aacccgctcc gccttcagcg ggcgcgacag cagggcgtcg atggcctgat cgacgctcat
     2101 gccgccggat aaaacggcgt tgaccgcgtt gcagagcggc aggtcgacgc cgttcttgcg
     2161 gccgaggtcg cagaccgccg gggccgactg cacgccctcg gcgaccgaca ccttgcccgc
     2221 cagcgccgcc tccagcgtct gcccttgtcc cagcgcgagg ccgacgctca tattgcgcga
     2281 ctgcggcgac gagcaggtca gcaccagatc gcccatgccg cacaaccccg ccatggtcgc
     2341 cagttgcgcg cccatcgcga tggccagccg cgtcatctcc gcgaagccgc gcgtgatcag
     2401 ggcggcgtgg gcgttgcggc ccag
//
LOCUS       DASVLL010000385         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_399858,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000385 DASVLL010000000
VERSION     DASVLL010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399858"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /gene="flhA"
                     /locus_tag="VF402_06365"
     CDS             <1..1862
                     /gene="flhA"
                     /locus_tag="VF402_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX7799953.1"
                     /translation="TVLLVTTLFRLALNITSMRIILTHGQDGEHAAGSVINAFGQLMT
                     QGNFIIGGILFSILVLANFVVITKGSGRIAEVSARFTLDSMPVKQMAIDADLSSGLID
                     GEEARKRRKEVELESSFFGAMDGASKFVRGDAVAGIVITLVNIVGGMLIGILQHGMDP
                     GQAAHTYILLTIGDGLVTQIPALIISIAAGFLVSKAGVDGSADKALVTQLATNPVALG
                     VVSAASGVIGLIPGMPVLFFGAIALGTGALAWRVGRKPPLPTPEEMAAQAAANAPPED
                     EPIATALAIDDVKIELGLGLLGLINDLDGRKLTDQIKALRRTLAQEYGFVMPSVRILD
                     NMRLNNQGYCVRIKEMEAGAGEVRIGSLMAMDPAGRQIELPGEHTREPAFGLPATWID
                     NALREEATFLGYTIVDPATVITTHLTEILKDNMADLLSYGELTKLLRDLPADEKKLAD
                     DLIPATVSVATLQRVLQALLKERVSIRDLSAILEALGEAAAHTKSVTQMVEHVRSRLA
                     RQLCWQNRAEDGTLPIVTLSHEWENAFASSLIGQGEERQLTMAPSQLQEFIRAVRDTF
                     ERVSMSGEVAVLLTSPLIRPFVRSIIERFRAATVVMSQNEIHPKVKLKTVGQI"
     gene            complement(1869..2201)
                     /gene="mazF"
                     /locus_tag="VF402_06370"
     CDS             complement(1869..2201)
                     /gene="mazF"
                     /locus_tag="VF402_06370"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HEX7799954.1"
                     /translation="MSYIPDQGDIVWLDFDPQAGHEQAGRRPAIVLSPAAYNNRTSLM
                     VCCPMTTKIKGNPWEVRIAAHREGVVLSDQIKSLDWTARRIEKTGEATAAELGQVRKF
                     ARALIGRP"
     gene            complement(2198..2470)
                     /locus_tag="VF402_06375"
     CDS             complement(2198..2470)
                     /locus_tag="VF402_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7799955.1"
                     /translation="MFKHGGHAVLTTLKKWGNSPSVRIPADIMRRANLHIDQKVEVEL
                     VDGVIQIRSAETGEEADLDAMIAQLTPDNLHDFVGTGPARGMETGE"
     gene            2602..>3048
                     /locus_tag="VF402_06380"
     CDS             2602..>3048
                     /locus_tag="VF402_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HEX7799956.1"
                     /translation="MKRVMRQVFGSTRPKDIWLDLWTFDRLNAQALVHIVYWAGLGLL
                     MIAAFGVAGVAVGNAWADGSLWGYVLALPMIVIGWLGVVIGIVLWRVLCEFILSVMGI
                     AEDLRVLRQYQEKLTPMEPQGAPAPVQAPVEAVETAPEMAAPVFTPD"
BASE COUNT          475 a          956 c         1057 g          560 t
ORIGIN      
        1 cgaccgtgct tctggtcacc accctgttcc ggctggcgct caacatcacc tcgatgcgca
       61 tcatcctgac gcacgggcag gacggcgaac acgcggcggg gtcggtgatc aacgccttcg
      121 ggcagttgat gacgcagggc aatttcatca tcggcggcat cctgttctcg attctggtgc
      181 tggcgaactt cgtcgtcatc accaagggtt cgggccgcat cgccgaagtg tcggcgcgct
      241 ttaccctcga ttccatgccg gtcaagcaga tggccatcga cgccgacctg tcgtccggtc
      301 tgatcgacgg cgaggaggcg cgcaagcggc gcaaagaggt cgagctggaa tcgagcttct
      361 tcggcgccat ggacggcgcc tcgaagttcg tgcgcggcga cgcggtggcg ggcatcgtca
      421 tcacgctggt caatatcgtc ggcggcatgc tgatcggcat tttgcagcac ggcatggacc
      481 ccggtcaggc ggcgcacacc tatatcctgc tgaccatcgg cgacggcctc gtcacgcaga
      541 ttccggcgct gatcatctcg atcgccgccg gtttcctcgt ctcgaaggcg ggcgtggacg
      601 gttcggcgga caaggcgctg gtcacgcaac tggcgaccaa tccggtggcg ctgggcgtgg
      661 tgtcggcggc gtcgggcgtg atcgggctga tccccggcat gccggtgctg ttcttcggcg
      721 ccatcgcgct ggggaccggc gcgctggcct ggcgcgtggg gcgcaagccg ccgctgccca
      781 cgccggagga gatggccgcg caggccgccg ccaacgcgcc gccggaggac gagccgatcg
      841 cgaccgcgct ggccatcgac gatgtgaaga tcgagctggg gctggggctg ctgggcctga
      901 tcaacgatct ggacgggcgc aagctgaccg accagatcaa ggcgctgcgg cggacgctgg
      961 cgcaggaata cggcttcgtc atgcccagtg tgcgcatcct cgacaatatg cgcctgaaca
     1021 atcagggcta ttgtgtgcgc atcaaggaga tggaggccgg cgccggcgag gtgcgcatcg
     1081 ggtcgctgat ggcgatggac ccggcggggc ggcagatcga actgccgggc gagcatacgc
     1141 gcgaaccggc cttcggcctg cccgcgacct ggatcgacaa cgcgctgcgc gaggaggcga
     1201 ccttcctcgg ctacaccatc gtcgatccgg cgaccgtgat caccacgcac ctgaccgaaa
     1261 tcctcaagga caatatggcc gacctgttgt cctatggcga gctgaccaag ctgctgcgcg
     1321 acctgcccgc cgacgagaag aagctggccg acgacctgat cccggccacc gtctcggtcg
     1381 ccaccttgca gcgcgtgttg caggccctgc tcaaggagcg cgtctcgatc cgcgacctgt
     1441 cggccattct ggaggcgctg ggcgaggcgg cggcgcacac caagtcggtg acccagatgg
     1501 tcgagcatgt gcgctcgcgg ctggcgcggc aactgtgctg gcagaaccgc gccgaggacg
     1561 ggacgctgcc catcgtcacc ctgtcgcacg aatgggagaa cgcctttgcg tcctcgctga
     1621 tcgggcaggg cgaagagcgg caactgacca tggcgcccag ccagttgcag gagttcatcc
     1681 gcgccgtgcg cgacacgttc gagcgcgtgt cgatgtcggg cgaggtggcc gtgctgctga
     1741 cctcgccgct gatccgcccg ttcgtgcgct cgatcatcga acgcttccgc gccgccaccg
     1801 tggtgatgag ccagaacgaa atccacccca aggtgaagct caagacggtc gggcagatct
     1861 gacgacggct atggccgtcc gatcagggcg cgggcgaatt tgcgcacctg gccgagttcg
     1921 gcggccgtgg cctcgccggt tttctcgatg cggcgggcgg tccagtcgag gctcttgatc
     1981 tgatccgaca gcacgacgcc ttcgcggtgc gccgcgatgc ggacctccca cggattgccc
     2041 ttgatcttcg tcgtcatcgg gcagcacacc atcagcgagg tgcggttgtt ataggcggcg
     2101 gggctgagga cgatggccgg gcggcggccc gcctgttcgt gtcccgcctg cggatcgaag
     2161 tcgagccaga cgatgtcgcc ctgatcggga atgtaactca ttcgcccgtt tccatgccgc
     2221 gcgccgggcc cgtgccgacg aaatcgtgca ggttgtcggg tgtaagctgc gcgatcatgg
     2281 cgtcgagatc ggcctcctcc ccggtttcgg cggagcgaat ctggatcacg ccgtccacca
     2341 gttcgacctc gaccttctgg tcgatatgca ggttggcgcg ccgcatgatg tcggcgggga
     2401 tgcgcacgga ggggctgttg ccccatttct tcagcgtggt cagcacggcg tggcctccat
     2461 gtttaaacat tgtcgatact ttagcatgat cgtccgtgcg tcgcaacatt gtatcgacgc
     2521 gccgccgctc ttgacatttg ccgcggctga ccgttttagc ttgggggaca gcaggcaatt
     2581 tcgcctgtca ggggatagcc tatgaagcgt gtgatgcggc aggtgttcgg ttcgacgcgg
     2641 ccgaaggata tctggctgga tctatggacc ttcgaccgtc tcaatgcgca ggcgttggtg
     2701 catatcgtct attgggcggg gctgggcctg ctcatgatcg ccgctttcgg cgtggcgggc
     2761 gtggcggtcg gcaatgcgtg ggcggacggc tcgctgtggg gctatgtgct ggccctgccg
     2821 atgatcgtga tcggctggct gggcgtcgtc atcggcatcg tcctgtggcg cgtgctgtgc
     2881 gaattcatcc tgtcggtcat gggcatcgcc gaggatctgc gcgtgctgcg ccagtatcag
     2941 gaaaagctga ccccgatgga gccgcaaggc gcgccggcgc cggttcaggc cccggtcgag
     3001 gcggtggaga cggcgccgga aatggccgcg cccgtcttta cgccggac
//
LOCUS       DASVLL010000386         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_455273,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000386 DASVLL010000000
VERSION     DASVLL010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455273"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF402_06385"
     CDS             <1..362
                     /locus_tag="VF402_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7799957.1"
                     /translation="GYACALFAKENPIEPTVLEIIDIQALACAPDAVATLMDALMAAG
                     RQRGAAKLRLSMYGPQLFERLGRHRDAARREGGWPHAHVRAAPGAPALDDWSPTPFDG
                     DLFMSLRAPPIPLRQSA"
     gene            450..1298
                     /locus_tag="VF402_06390"
     CDS             450..1298
                     /locus_tag="VF402_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7799958.1"
                     /translation="MTHIDLPANFAFLAGLHINPLFILSGLLVGALVGLTGVGGGSLM
                     TPILVLLFGVHPATAVGTDLLYASITKGVGTAIHGARRTVDWRIVGLLAIGSIPATLL
                     TLFGIEHCGKSAAMSSLITHVLGYALFLTAVMLIMRGRIRRYAERQQAEALAAGADEG
                     RLRARRIALTIVVGLVLGFLVSLSSVGAGALGVTALILMYPQLPTVRIVGSDIAHAVP
                     LTFLAGAGYFLLGKVEVSMLISLLLGSVPGIIAGSLLAPRLPEKALRVMLAAVLALVG
                     LRLVLG"
     gene            complement(1299..1796)
                     /locus_tag="VF402_06395"
     CDS             complement(1299..1796)
                     /locus_tag="VF402_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799959.1"
                     /translation="MPALLPRASTLLKAAIAAGAAIATGLLLSQCGQPKASANGFDVA
                     LSFTPAASAKLKALGQKAVVDAYFYGLPTEATAAKVDEEGHINLGESFASADGADQTT
                     RIDANAIDPAMMDKIKKDSAAVTVRAYLDPTAGVANMLDCSIFNGPLIAAQARPVAIR
                     CDLMK"
BASE COUNT          264 a          656 c          689 g          366 t
ORIGIN      
        1 tgggctatgc ctgcgcgctg ttcgccaagg agaatccgat cgagccgaca gtgctggaga
       61 tcatcgacat acaggcgctg gcgtgcgcgc cggacgcggt cgcgacgctg atggacgcgc
      121 tgatggcggc gggacggcag cgcggcgcgg ccaagctgcg cctgtccatg tacggcccgc
      181 aactgttcga gcggctgggc cgccaccgcg acgcggcgcg ccgcgaaggc ggctggccgc
      241 acgcccatgt ccgggccgcg cccggcgcgc cggcgctgga cgactggtcg ccgacgccgt
      301 tcgacggcga cctgttcatg agcctgcgcg cgccgccgat tccgctgcgt caatccgcct
      361 aacggatttc gtgaattaaa ttcatttgtc ttgcgcgccg ccctccgtaa tgtgaggctc
      421 gaaaaacgca acgggacaca ctcggcctta tgacgcatat cgacctgccg gccaatttcg
      481 cctttctggc cgggctgcat atcaacccgc tgtttatcct gtcgggcctg ctggtcggag
      541 cgttggtcgg gctgaccggc gtcgggggcg ggtctttgat gacgccgatc ctggtgctgc
      601 tgttcggcgt gcatccggcc acggcggtcg gcacggacct tctgtacgcc tcgatcacca
      661 agggcgtcgg cacggccatc cacggcgcgc gccgcacggt cgactggcgc atcgtgggcc
      721 tgctggccat cggctccatt cctgcgacgc tcctgaccct gttcggcatc gagcattgcg
      781 gcaagagcgc ggccatgtcg tcgctgatca cgcacgtgct gggctatgcg ctgttcctga
      841 cggcggtgat gctgatcatg cgcggacgca tccgccgcta cgccgagcgc cagcaggccg
      901 aggcgctggc cgccggcgcc gacgagggca ggctgcgcgc gcgccgcatc gccctgacca
      961 tcgtggtcgg cctcgtgctg ggctttctgg tgtcgctgtc gtcggtgggc gccggcgcgc
     1021 tgggcgtgac cgcgctgatc ctgatgtatc cgcaactgcc gaccgtgcgc atcgtcggct
     1081 cggacatcgc ccatgccgtg ccgctgacct ttctggccgg cgccggttat ttcctgctgg
     1141 gcaaggtcga ggtgtcgatg ctgatctcgc tgctgctggg atcggtgccc ggcatcatcg
     1201 cgggcagcct tctggcgccg cgcctgccgg aaaaggcgct gcgcgtgatg ctggccgccg
     1261 tgctggccct ggtcggcctc aggctggtgc tgggctgatt acttcatcag gtcgcagcgg
     1321 atggcgacgg gcctggcctg ggcggcgatc agcgggccgt tgaagatgct gcaatccagc
     1381 atgttcgcca cgcccgccgt cgggtcgaga taggcgcgca ccgtcaccgc cgccgaatcc
     1441 ttcttgatct tgtccatcat ggccggatcg atggcgttgg cgtcgatccg cgtcgtctgg
     1501 tccgcgccgt cggcgctggc gaaggattcg ccgagattga tatggccttc ctcatcgacc
     1561 ttggccgccg tcgcctcggt cggcaggccg tagaaatagg cgtcgaccac ggccttttgg
     1621 cccagcgcct tcagcttcgc gctggcggcg ggcgtgaagc tcagcgcgac gtcgaagccg
     1681 ttggccgagg ccttcggctg gccgcactgg ctcagcagca gaccggtcgc gatggcggcg
     1741 ccggcggcga tggcggcttt gagcagggtg gaggcgcggg gcagaagggc gggcatggtc
     1801 actcggtctc ggtatgtcgg cgaagcgctg tcataaatca tccgcctccg cgaggccagc
     1861 ctaaacggtt gtcccattat tgcacgtcgc agatcagcgt gaccgggcgc gattgcgcgg
     1921 tcgccacata gtcctgaaag acgtcgcaac cgacgccgtc gcgcggcttg acctc
//
LOCUS       DASVLL010000387         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_132789,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000387 DASVLL010000000
VERSION     DASVLL010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132789"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1535
                     /locus_tag="VF402_06400"
     CDS             69..1535
                     /locus_tag="VF402_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7799960.1"
                     /translation="MNKFYLGGAAALALCIGAVAYASQPAFSPKSEDYEQLALFGNVL
                     SLVEKDYVVKVDSKKLISAALQGMLASLDPHSNYLDGDDYTDLKEKTRGSYGGIGLEV
                     TGEDGAVKAVTAMDDTPAQKAGIESGDYITAIDGTSILGLPLNDAVSKMKGDPGSKLT
                     LTIVRTGKDQPFDVTLTREIITVKSVKTRMEGDYGYLRIASFQENTAAEAQAALSDLM
                     KQNPKMKGLILDLRNNPGGLLEQSVGVADMFLNGGEIVSQRGRQPDDIIRYQAKKGDM
                     LNGLPLVVLTNGGTASAAEIVSGALQDQHRAEIVGETTFGKGSVQSVIELGDNRAVKL
                     TTARYYTPSGRSIQKTGITPDLVVAQSRDQAKYIATAALQYTEAAYANALDADEGKKR
                     VPPVEQEVPPDGFDTKKGDFELQRGLDVLAAGGNVAEVRAHPKGVVLLTADLADKPGS
                     RFANLTKAGASSADSSSAASASASGSAASSSDASASSN"
BASE COUNT          321 a          559 c          509 g          245 t
ORIGIN      
        1 cgccacagtc acactcctat atgaaggtcg tgacttccgc ttcgcggccc cctcttcaga
       61 gatattgaat gaacaagttc tatcttggcg gcgcggccgc cctggcgctg tgcatcggcg
      121 ccgtggccta cgccagccag cccgccttct cgcccaaatc cgaggactat gagcaactgg
      181 ccctgttcgg caacgtcctc agcctggtcg agaaggacta tgtggtcaag gtcgattcca
      241 agaagctgat ctccgccgcg ctgcagggca tgctggccag cctcgatccg cattccaact
      301 atctcgacgg cgacgactat accgacctga aggaaaagac gcgcggctcc tacggcggca
      361 tcgggcttga ggtcaccggc gaggacggcg ccgtcaaggc cgtgaccgcc atggacgaca
      421 ccccggcgca gaaggcgggc atcgaatcgg gcgactacat caccgccatc gacggcacct
      481 cgatcctcgg cctgccgctc aacgacgcag tcagcaagat gaagggcgac cccggttcga
      541 aactgaccct gaccatcgtg cgcaccggca aggatcagcc gttcgacgtc accctgacgc
      601 gcgaaatcat caccgtgaag tcggtcaaga cgcgcatgga gggcgattac ggctatctgc
      661 gcatcgcctc gttccaggag aacaccgccg ccgaggcgca ggccgcgctt tccgacctga
      721 tgaagcagaa cccgaagatg aagggcctga ttctcgacct gcgcaacaat ccgggcggcc
      781 tgctggaaca gtcggtgggc gtcgccgaca tgttcctcaa cggcggcgag atcgtctcgc
      841 agcgcgggcg ccagcccgac gacatcatcc gctatcaggc caagaagggc gacatgctga
      901 acggcctgcc gctggtggtg ctgaccaacg gcggcacggc ctcggcggcg gaaatcgtct
      961 ccggcgcgct tcaggaccag caccgcgccg agatcgtcgg cgaaaccacc ttcggcaagg
     1021 gctcggtgca gagcgtgatc gagctgggcg acaaccgcgc ggtcaagctg accacggcgc
     1081 gctattacac gccgtcgggc cgctcgatcc agaagaccgg catcacgccc gatctcgtgg
     1141 tcgcccagtc gcgcgatcag gccaagtaca tcgccaccgc cgccctgcaa tataccgaag
     1201 ccgcctacgc caacgcgctc gacgccgacg aaggcaagaa acgcgtcccg ccggtcgagc
     1261 aagaggttcc gcccgacggt ttcgatacga aaaagggcga tttcgagctg caacgcggcc
     1321 tcgacgtgct ggccgcgggc ggcaatgtcg ccgaggtcag ggcgcacccg aagggcgtgg
     1381 tcctgctcac cgccgacctg gccgacaagc cgggcagccg cttcgccaac ctgaccaagg
     1441 cgggcgcttc gagcgcggac agcagcagcg cggcctcggc gtcggcgagc ggtagcgcgg
     1501 cgtcttcgtc agacgcctcc gcctcatcga actgaaatcg ggcctcccgg cgacgggagg
     1561 ccttttctgt ttccgagaga aagttgccgt cgacgccgtc tgaaccgatc aagggcatgg
     1621 acgccgttta ccgc
//
LOCUS       DASVLL010000388         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_186045,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000388 DASVLL010000000
VERSION     DASVLL010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186045"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VF402_06405"
     CDS             complement(<1..947)
                     /locus_tag="VF402_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7799961.1"
                     /translation="MDRKSYNLYAVILHWLIAGLILFMIFLGWRLGDHDSMRLSRVNL
                     HKSVGISILLLSLVRLGLRLAYKAPPEPPMPKWQLWAAKALHVGFYIVMIGMPLTGWL
                     MVSTSAREIPFFLWHWPHLPVPQNHATHEFFEGLHGLIAKLIIYVMVPLHVLAALKHQ
                     FVDKDEVMRRMVPGLTPKPVMNWRWIVPVGVVALAVGLGFGVYRGVPEKGGEKQAAAP
                     AASMQAPPAASETASPEASVASSSGSAAAVPVWAVDKAASKIAFATTFSGEAINGAFS
                     GYNAVIAFDPARLGASHVRVSIDLASVASGDGDRDSSLR"
     gene            1122..>2391
                     /locus_tag="VF402_06410"
     CDS             1122..>2391
                     /locus_tag="VF402_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7799962.1"
                     /translation="MTYRPSLKDIRFCLDHVIGMDAHYSSAAFPDFDADLRDAVLDAA
                     GQLAGDVLAPLNRVGDLHGAKLEGDRVVVAPGFLEAIRTYADGGWYGLAAEPEHGGQG
                     LPKMLEQACFSLFHAANMAFTLLPTLSQAAIDALQIHGTAQQQALYLPRLISGEWLGT
                     MNLTEPGAGSDLAALKTRAERNADGSYRLTGQKIFITWGEHDGAANILHLVLARLPDA
                     PPGTRGISLFLCPKYEVAPDGVLGARNAVQCVGLEHKMGIHASPTCVMAYEDARAELI
                     GAENGGLAAMFTMMNAARLAVGFEGVGLADAAWRQAQGYAQERRQGRSPITGEDYAPI
                     RDHPDVRLMLGLMKAKGEAARAVCMATAAAVDAARMAGDEASRQRHRRREDVLVPIAK
                     AWSTDRAVEAASLGVQVHGGMGFIEETGAAQ"
BASE COUNT          426 a          800 c          809 g          356 t
ORIGIN      
        1 ctgcgcaggc tggaatcgcg gtcgccgtcg ccggacgcca ccgaggccag atcgatgctc
       61 acccggacat gcgaggcgcc cagtctggcg ggatcgaagg cgatgacggc gttatagccg
      121 gaaaaggcgc cgttgatcgc ctcgcccgaa aaggtcgttg caaaggcgat cttgctggcg
      181 gccttgtcga ccgcccagac ggggaccgcc gccgcgctgc cggacgatga cgccacggac
      241 gcttccggcg aagcggtttc ggacgccgcc ggcggagcct gcatcgaggc ggcaggcgcc
      301 gccgcttgct tttcaccgcc cttttccggc acgccgcgat agacgccgaa gcccagcccc
      361 acggccagag cgaccacgcc caccggcacg atccagcgcc agttcatcac cggcttcggc
      421 gtcaggcccg gaaccatgcg ccgcatcacc tcgtccttgt cgacgaactg gtgcttgagc
      481 gccgccagca cgtgcagcgg gaccatgaca tagatgatca gcttggcgat caggccgtgc
      541 agcccctcga aaaactcatg cgtggcgtgg ttctgcggca cgggcaggtg cggccaatgc
      601 cagaggaaga acggaatctc gcgcgcgctg gtcgagacca tcagccagcc ggtcagcggc
      661 atgccaatca tcacaatgta aaagccgaca tgcagcgcct tggccgccca tagctgccat
      721 ttcggcatcg gcggctccgg cggcgccttg tacgccaggc gcaggccgag gcgcacgagg
      781 ctcaaaagca ggatcgatat gccgaccgac ttgtgcagat tgacccggct caggcgcatc
      841 gaatcgtggt cgcccagccg ccagcccaga aagatcatga acaggatcag tccggcgatc
      901 agccagtgca ggattacggc gtaaagattg tagctcttgc gatccatgcg cgcctcctcg
      961 tcgttccggc atacaccaac cgtcgcacag cgtcatgcgg atttatatgt aaccgctata
     1021 acttcatttt agccgccaaa agttgacgtt cacgtaaaac aacttgccaa gcccggaacg
     1081 cggcgcctac tatgacggca taaacatcag ggacttaaag catgacctac cggcccagcc
     1141 tcaaggacat ccgtttctgc ctcgaccatg tgatcggcat ggacgcgcat tacagcagcg
     1201 ccgcctttcc cgacttcgac gcggacctgc gcgacgcggt gctggacgcc gccgggcagt
     1261 tggcgggcga cgtgctggcg ccgctgaacc gcgtcggcga cctgcacggc gcgaagctgg
     1321 agggggatcg cgtcgtcgtc gcgccgggct tcctcgaagc gatcagaacc tatgccgacg
     1381 gcggctggta cgggctggcc gccgagccgg aacatggcgg tcagggcctg cccaagatgc
     1441 tggagcaggc gtgtttcagc ctgttccacg ccgccaacat ggccttcacc ctgctgccga
     1501 ccctgagtca ggccgccatc gacgccttgc agattcacgg cacggcgcag cagcaggcgc
     1561 tttatctgcc ccggctgatc tccggcgaat ggctggggac gatgaacctg accgagccgg
     1621 gcgccgggtc cgatctggcg gcgctgaaaa cacgggcgga gcgcaacgcg gacggctcct
     1681 atcgcctgac cgggcaaaag atcttcatca cctggggcga gcatgacggc gcggccaata
     1741 tcctgcatct ggtgctggcg cggctgcccg acgcgccgcc ggggacgcgc ggcatctcgc
     1801 tgttcctgtg cccgaaatac gaggtggcgc cggacggcgt tctgggcgcg cgcaacgccg
     1861 tgcagtgcgt cgggctggag cacaagatgg gcatccacgc ctcgccgacc tgcgtgatgg
     1921 cctatgagga cgcgcgcgcc gaactgatcg gcgccgagaa cggcgggctg gcggccatgt
     1981 tcaccatgat gaacgcggcg cggctggcgg tcggcttcga gggcgtgggg ctggccgacg
     2041 cggcgtggcg tcaggcgcag ggctatgcgc aggagcgccg tcagggccgc tcgccgatca
     2101 ccggcgagga ctatgcgccg atccgggacc atcccgacgt gcgcctgatg ctggggctga
     2161 tgaaggcgaa gggcgaagcg gcgcgcgccg tctgcatggc gaccgccgcc gccgtcgacg
     2221 ccgcgcgcat ggccggcgac gaggcgagcc gccagcgcca caggcggcgc gaggacgtgc
     2281 tggtccccat cgccaaggcg tggagcacgg accgcgccgt cgaggccgcc tcgctgggcg
     2341 tgcaggtcca cggcggcatg ggcttcatcg aggagaccgg cgcggcgcaa t
//
LOCUS       DASVLL010000389         6679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_239058,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000389 DASVLL010000000
VERSION     DASVLL010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239058"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VF402_06415"
     CDS             complement(<1..478)
                     /locus_tag="VF402_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family chaperone protein"
                     /protein_id="HEX7799963.1"
                     /translation="MTYKAEAPSKASDSLGYRPKRFWREVAVVSGADGFEVRLDGRAV
                     RTPQGRLMTLPNAALAEAAAAEWAAVAEHVDYAHMPLTRLAFVAVDRMDETREAARAE
                     ALRYAETDLTCYPSDYPQGLIAREDAAWRPVLAWAQAEMGLRFHQNKTLIHQPQPAE"
     gene            complement(480..1178)
                     /locus_tag="VF402_06420"
     CDS             complement(480..1178)
                     /locus_tag="VF402_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7799964.1"
                     /translation="MTLKLAVWDVDGTLVDSRASILRALQEGAVAVGLTPPSYDEARH
                     IVGLSLLEALQTLKPEISAEHAEAYAHQYKESFIRFHAEAGFHEPLYDGADAALRRLK
                     REGWRLGMATGKSRRGIERNEEFFGWQGVFDATFCGDDGPSKPDPFMIERNMETVGAA
                     PHETVMIGDTGHDIGMARAAGVYAIGVSWGFHTVAELEAAGAHEIVHDYATLNARLDA
                     FAAQIADRKTAIRV"
     gene            complement(1229..2233)
                     /locus_tag="VF402_06425"
     CDS             complement(1229..2233)
                     /locus_tag="VF402_06425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7799965.1"
                     /translation="MSKDVKTLYVADGEDGVRVDRFFKRRWPHVNHVQLNKLLRSGQV
                     RVDGGRVKADTRLVAGQTVRVPPLPDAPDADQQKRERATLSPQEIRYAHSLVLYEDED
                     VIALNKPSGLAVQGGTKTRNHVDRLLSAWGEGLERPRLVHRLDRDTSGVLLLGKSPQA
                     AARLAGAFAKRRAHKTYWAVVVGNPKPEEGFIDTPLVKKGFHDREIVQPAEANEPGAE
                     PAETEYVTIARAGPRAAWMALRPHTGRTHQLRAHMQAIGHSILGDPKYGSDASRDLSE
                     GLGLQLHHRRIELPHPTQGTLIVEAPLDPVIKAGFERFGFDWHEAEADPFVHMKKKRR
                     "
     gene            complement(2252..2674)
                     /gene="crcB"
                     /locus_tag="VF402_06430"
     CDS             complement(2252..2674)
                     /gene="crcB"
                     /locus_tag="VF402_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX7799966.1"
                     /translation="MLANALIVMIGGAFGSLARYLLGVYSARWWVRLDWPFNEWPLGT
                     FLANLVGGLVMGLVMGLLFGPLKDVVASDHVERWRLLLAVGVLGGFTTFSSFSLETVL
                     MLERRAYVLAGLYVSLSVILSIAAVALGLFVTRRIVAG"
     gene            2753..3184
                     /locus_tag="VF402_06435"
     CDS             2753..3184
                     /locus_tag="VF402_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX7799967.1"
                     /translation="MTLTLTYDPGNIFAKIIAGDIPCARVYEDARVLSFMDAFPQSKG
                     HTLVVPKVPVCNLFDIADDDLANLIVQTRRIARAVREALAPDGLRLIQFNGEAAGQTV
                     FHLHFHILPIWAGQPLSPHGAGGMADMEVLKAAAARIADRL"
     gene            3184..3810
                     /locus_tag="VF402_06440"
     CDS             3184..3810
                     /locus_tag="VF402_06440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7799968.1"
                     /translation="MSAFYVLRHGQTDWNVAARLQGSTDIPLNATGREQARKAAEILA
                     GEAITRIIASPLSRALETARIVGARIGVEPETDARLIERHFGLFEGMTIDEVRRHREE
                     MRDFMNPAADIDGKHYPDNAEPLPAVIDRVFACVDALRAAGRCLFVCHGIPFRAISRK
                     FLDDMYSSPNAAPVRFEETGGVWRMIALDPGNAPIQQIYNAQTTMGRI"
     gene            4199..4807
                     /locus_tag="VF402_06445"
     CDS             4199..4807
                     /locus_tag="VF402_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEX7799969.1"
                     /translation="MTDNKLFDPRGFAARLRGLIHPEKHAAFCERAGISLSVLNKYLA
                     APDGLSPSAEMLARICRATRASLDWLIMGEGDSGRTSDTIVNIPACDDPDDGMPFTRA
                     FLARLGYAAPESLCVLHAEGDSMEPLITDGAPVLIDRSDARLREGVFAFRIGEHVHVK
                     RLRPAGSGGVEILPANPAYPPETLPDGRFVLLGRAIWTGNDI"
     gene            complement(5025..6017)
                     /locus_tag="VF402_06450"
     CDS             complement(5025..6017)
                     /locus_tag="VF402_06450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7799970.1"
                     /translation="MIPFSVLDLSQIGEGSTVSDALANSARLAQAAEAHGYARFWLAE
                     HHGMPGIASAATAVAIAHVGHATKTIRVGAGGIMLPNHAPLMVAEQFGTLEALFPGRV
                     DLGLGRAPGTDMATARALRRNLEQGVDSFPQDVLELQAYLGAPVEGQRLLAVPGMNSH
                     VPIWILGSSLYGAQLAAQLGLPYAFASHFAPDMLFEALHVYRSTFRPSEHLQKPHVMV
                     GVMGVAADTDAEAEFLYTSMQQSFANLRRNVRAPFPKPSYDAVADLTADERVMIDHML
                     RYALVGSPATIAPRLASFLERTQADELIVSMPVHAIEARLHALALFAQVRKNLA"
     gene            6161..>6679
                     /locus_tag="VF402_06455"
     CDS             6161..>6679
                     /locus_tag="VF402_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7799971.1"
                     /translation="MEYRQLGRSGLKVPVLSYGTGTFGGRGDFFGKWGDSDVLEADRL
                     VNICLEHGLNFFDTADVYSKGASEEILGAALKGRRDKALISTKATFSMGDGPNDKGSS
                     RWHLIRAAEASLRRLNTDHIDVYFMHGYDALTPVEEMLSALDHLISSGKVRYIGASNY
                     SGWHLMKALATSE"
BASE COUNT         1166 a         2362 c         2078 g         1073 t
ORIGIN      
        1 tttccgccgg ttgcggctga tggatcagag tcttgttctg gtgaaaacgc agccccatct
       61 cagcctgcgc ccacgccaga accggccgcc acgccgcgtc ctcgcgcgcg atcaaccctt
      121 gcggatagtc ggacggatag caggtcaggt cggtttcggc gtaccgcagc gcctcggccc
      181 gcgccgcctc gcgcgtctcg tccatgcggt cgacggcgac gaaggccagc cgcgtcagcg
      241 gcatgtgcgc gtagtcgaca tgctccgcga ccgccgccca ttccgccgcc gccgcctcgg
      301 ccagcgccgc attcggcagg gtcatgagtc tgccctgcgg cgtccgaacc gcgcggccgt
      361 cgagacgcac ctcgaagccg tccgcgccgc tgacgacggc gacctcgcgc cagaagcgct
      421 tagggcgata gcccagcgag tccgaggctt tggagggcgc ttcggcttta tacgtcatat
      481 caaaccctta tcgccgtttt ccgatccgcg atctgagccg cgaaggcgtc gagccgcgcg
      541 ttcagcgtcg cgtaatcgtg tacgatctcg tgcgcgcccg ccgcctccag ctccgcgacg
      601 gtgtggaagc cccacgacac gccgatggcg taaacccccg ccgcgcgcgc catgccgata
      661 tcgtggccgg tgtcgccgat catcaccgtc tcgtgcggcg cggcgcccac cgtctccata
      721 ttgcgctcga tcatgaacgg gtcgggcttg gacggcccgt cgtcgccgca gaaggtcgcg
      781 tcgaacacgc cctgccagcc gaagaattcc tcgttgcgct cgatgccgcg ccgcgacttg
      841 cccgtggcca tgcccagccg ccagccctcg cgcttcaacc ggcgcagggc cgcgtcggcg
      901 ccgtcgtaca gcggctcgtg aaagcccgcc tcggcgtgaa agcggatgaa gctttccttg
      961 tactgatgcg cgtaagcctc ggcgtgttca gcggaaatct ccggcttcag ggtttgcagc
     1021 gcctccagca gcgacaggcc gacgatgtgg cgcgcctcgt catagctggg cggcgtcagc
     1081 ccgaccgcga cggcgccctc ctgcaaggcg cgcaggatcg aagcccggct gtcgaccagc
     1141 gtcccgtcaa cgtcccacac ggcaagttta agcgtcatga tactctactt cttatggatg
     1201 cgtgttcgtc acacgtcgca acggcgtctt accgtcgctt cttcttcata tggacgaacg
     1261 gatcggcttc ggcctcatgc caatcgaagc cgaaacgctc gaagccggcc ttgatgaccg
     1321 gatcgagcgg cgcctcgacg atcagcgtcc cctgcgtcgg gtgcggcagt tcgatgcgcc
     1381 ggtgatgcag ttgcagccct aagccttcgg acaggtcgcg gctggcgtcg gagccgtatt
     1441 tcggatcgcc gaggatggaa tggccgatgg cctgcatatg ggcgcgaagc tggtgcgtgc
     1501 gcccggtgtg cggccgcagg gccatccacg ccgcgcgcgg cccggcgcgc gcgatggtga
     1561 cgtattcggt ttcggcgggc tccgcgcccg gctcgttcgc ttcggcgggc tggacgatct
     1621 cgcggtcgtg aaagcccttc ttgaccagcg gcgtgtcgat gaagccctct tccggcttcg
     1681 gattgccgac caccaccgcc cagtaggtct tgtgcgcgcg gcgcttggcg aacgcgccgg
     1741 ccagccgcgc cgccgcctgc ggcgacttgc ccagcagcag cacgcccgac gtgtcgcggt
     1801 cgagacggtg gaccaggcgc ggccgctcca gcccctcgcc ccaggccgac aagagccggt
     1861 cgacatggtt cctggtcttg gtgccgccct gcacggccag ccccgacggc ttgttgagcg
     1921 cgatcacgtc ctcgtcctca taaaggacca gcgaatgcgc gtagcggatc tcctgcggcg
     1981 acagcgtcgc gcgctcgcgc ttctgctgat ccgcatccgg cgcgtccggc aggggcggca
     2041 cgcgcaccgt ctggcccgcg acaagccgcg tatcggcctt gacccgcccg ccgtcgacgc
     2101 gcacctgacc ggagcgcagc agcttgttga gctgcacgtg attgacgtgc ggccagcgcc
     2161 gcttgaagaa tcggtcgacg cgcacgccgt cctcgccgtc ggcgacgtac agggtcttga
     2221 cgtccttact cataccaaat tacccgccac attacccggc cactatcctg cgcgtcacga
     2281 acaagcccaa cgccacggcg gcgatcgaca ggatcaccga caaactcaca tacaaccccg
     2341 ccaggacata ggcgcgacgc tccagcatca gcaccgtctc cagcgaaaac gacgaaaagg
     2401 tcgtgaaccc gcccagcacg ccgaccgcca gcagcaaccg ccagcgctcg acatgatccg
     2461 aggcgaccac gtccttcagc ggcccgaaca gcaggcccat gaccagaccc atgaccagcc
     2521 cgccgacgag gttggccaga aacgtgccca gcggccattc gttgaacggc cagtccagac
     2581 gaacccacca tctggcgctg taaacgccga gcagatagcg cgccagcgaa ccgaaggcgc
     2641 cgcctatcat gacgatgagt gcattggcaa gcatcggaca gtcttacccc ccttggcgct
     2701 tcagagatag tcctctaagc tttttatcgg acccggatta cggaatcatc ccatgaccct
     2761 gaccttgacc tacgatcccg gcaatatttt cgccaaaatc atcgccgggg acatcccctg
     2821 cgcgcgcgtc tatgaggatg cgcgcgtgct gagcttcatg gacgcctttc cgcagtcgaa
     2881 gggacacacg ctggtcgtgc ccaaggttcc ggtctgcaac ctgttcgaca tcgccgacga
     2941 cgatctggcc aacctgatcg tgcagacgcg ccgcatcgct cgcgccgtgc gcgaggcgct
     3001 ggcgccggac ggcctgcgcc tgatccagtt caacggcgag gcggcgggcc agaccgtgtt
     3061 ccacctgcat ttccatatcc tgccgatctg ggccggccag ccgctgagcc cgcacggcgc
     3121 gggcggcatg gccgatatgg aggtgctgaa ggcggccgcc gcgcgcatcg cggaccggct
     3181 gtaatgtccg ccttctacgt tttgcgccac ggccagaccg actggaacgt cgcggcgcgg
     3241 ctgcaaggct cgaccgacat tccgctcaac gccacgggcc gcgaacaggc gcgcaaagcg
     3301 gcggagattc tggccggcga ggccataacg cgcatcatcg ccagccccct gtcgcgcgcg
     3361 ctggagacgg cgcgcatcgt cggcgcgcgc atcggcgtcg agccggagac ggacgcgcgg
     3421 ctgatcgaac gccatttcgg cctgttcgag ggcatgacca tcgacgaggt gcgccgccac
     3481 cgcgaggaga tgcgcgactt catgaacccc gccgccgata tcgacggcaa gcactatccc
     3541 gacaacgccg agccgctgcc cgccgtgatc gaccgcgtgt tcgcctgcgt cgacgcgctc
     3601 cgcgcggcgg gccgctgcct gttcgtctgt catggcattc cgttccgggc catatcgcgc
     3661 aaattcctcg acgacatgta ctccagcccg aacgccgcgc cggtcaggtt cgaggaaacc
     3721 ggcggcgtct ggcgcatgat cgcgctcgac cccggcaacg cgccgatcca acaaatctac
     3781 aacgcccaga ccactatggg gagaatatag aggaaataag ggaggacgcg ccaacgtcct
     3841 ccccccggtt ttgtccgcgc cgcggccacc cggagagaga agcttcgcgc ggacaaactg
     3901 aacgcggcgc cgccgccgac agacattgcg cggcccggca atcgcggcta cgcttggcgt
     3961 gaacgtggac tcgccgccgc cgcaaggcag ccggcggttt gcgcgcatac ccctcaacgg
     4021 gccggatttc cgcgatccgc gtggagcgcg ccgtcgcgtc cgcgcagggc ctcgcctctt
     4081 cccctcggcc gcgttccggc cggcgcctca cacgaccgcg atattcacga aagtgaactc
     4141 gaaaatacac gatcatgaat atgcgtcaag ctaaatccta ccgcccacgg cgataaaaat
     4201 gaccgataac aagcttttcg acccgcgcgg tttcgcggcc cggctgcgcg gcctcatcca
     4261 cccggaaaag cacgcggcct tttgcgaacg cgcgggcata agcctgtccg tcctcaacaa
     4321 atatctggcg gcgccggacg gcctgagccc cagcgccgag atgctggcgc gcatctgccg
     4381 ggccacgcgc gcaagcctgg actggctgat catgggcgaa ggcgattccg ggcggacctc
     4441 cgacacgatc gtgaacattc cggcctgcga cgatcccgat gacggcatgc cgttcacccg
     4501 cgccttcctc gcccggctgg gctatgcggc gccggaatcg ctgtgcgtcc tccatgccga
     4561 gggcgattcg atggagccgc tgatcacgga cggcgcgccg gtgctgatcg accggagcga
     4621 cgcgcgcctg cgcgaaggcg tcttcgcctt ccgcatcggt gagcatgtgc atgtcaagcg
     4681 cctgcggccc gccggttccg gcggcgtgga gatcctgccc gccaatccgg cctatccgcc
     4741 cgaaacgctg ccggacggcc ggttcgttct gctgggccgc gccatatgga ccggcaacga
     4801 catctagcgc gttccgaagc ggccaacagc gccgaaacga ttaagggagg acgcgccaac
     4861 gccctccccc cgatttgccc gcgccgcggc cacccggaga gagaagcttc gcacggacaa
     4921 aactgaacgt cgttatcggc gctattgtcg caagcagagg ttaaaaacaa ggacaactgc
     4981 aacgagtttg caattttttg catcttttcc cgatgaaagc ggttttaagc taaatttttc
     5041 cgcacctgcg cgaacagggc cagcgcatgc agccgcgcct cgatggcgtg gaccggcatg
     5101 gagacgatca gctcgtccgc ctgagtccgc tcaaggaagg acgcaaggcg cggcgcgatc
     5161 gtggcgggcg atccgaccag cgcgtaacgc agcatgtggt cgatcatcac ccgctcgtcc
     5221 gccgtcaggt ccgccaccgc gtcatagctc ggcttcggaa acggcgcgcg cacgttgcgg
     5281 cgcaggttgg cgaaggactg ctgcatcgag gtgtagagga actcggcctc ggcgtccgtg
     5341 tccgccgcca cgcccatcac gccgaccatg acgtgcggct tttgcagatg ctccgacggc
     5401 ctgaaggtcg agcgatagac gtgcagcgcc tcgaacagca tgtccggcgc gaaatgcgag
     5461 gcgaaggcgt agggcaggcc cagttgcgcc gccagttgcg cgccgtagag gctggagccc
     5521 agaatccaga tcggcacatg gctgttcatc cccggcacgg ccagcaggcg ttggccctcg
     5581 acaggcgcgc cgagataggc ctgcaactcc agcacgtcct gcgggaagct gtcgacgccc
     5641 tgctccaggt tccggcgcag cgcccgcgcc gtcgccatgt cggtccccgg cgcgcggcca
     5701 agccccagat cgacgcgccc cggaaacaac gcctccagcg tgccgaactg ctcggccacc
     5761 atcaagggcg cgtgattcgg cagcatgatc ccgcccgcgc cgacgcggat ggtcttcgtc
     5821 gcgtggccga catgcgcgat cgccaccgcc gtcgccgccg aggcgatgcc cggcatgccg
     5881 tgatgctcgg ccagccagaa gcgcgcatag ccgtgcgcct ccgccgcctg cgccagccgc
     5941 gccgaattgg ccagcgcgtc ggatacagtg ctgccctcgc cgatttgcga caggtcgaga
     6001 acggaaaacg ggatcatgcg gcggctcctt taccgtcaat gtaggcgcga cggttacgtt
     6061 ttccacatgc ggcccgcata aattccgccg gacggtttga aatccgccgc cgcgctcccc
     6121 aactcaggga ccatccccga agccgcaaag gagtcccgtc atggaatacc gtcagcttgg
     6181 ccggtccggc ctgaaagtcc cagtcctgtc ctacggaacc ggcaccttcg gcggacgcgg
     6241 cgacttcttc ggcaaatggg gcgacagcga tgttctggag gccgaccggc tggtcaatat
     6301 ctgcctcgaa cacggcctca acttcttcga cacggcggat gtctattcca aaggcgcgtc
     6361 ggaggaaatc ctcggcgccg cgctcaaagg ccgccgcgac aaggcgctga tctcgaccaa
     6421 ggcgacgttt tcgatgggcg acggcccgaa cgacaaaggc tcgtcgcgct ggcacctgat
     6481 ccgcgccgcc gaagccagcc tgcgccgcct gaacaccgac catatcgacg tctatttcat
     6541 gcacggctat gacgccctca cgccggtcga ggaaatgctg tcggcgctcg accacctgat
     6601 ctcgtccggc aaggtgcgtt atatcggcgc ctccaactat tccggctggc acctgatgaa
     6661 ggcgctggcg acctccgaa
//
LOCUS       DASVLL010000390         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_346628,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000390 DASVLL010000000
VERSION     DASVLL010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346628"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VF402_06460"
     CDS             <1..967
                     /locus_tag="VF402_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX7799972.1"
                     /translation="HARTRAMQAGAGSDDPVLARLTAAADALDRTLIAADEALALMDQ
                     AAESLDVEPGQLDRVEERLFALRAMARKLGVLVEDLPKERARIARSLNEIEDLDAHLR
                     RLDLAIHAAEKDFHAAVEALRARRQAAGAALSAAVMAELTPLKLDKAKFRVQVAALEP
                     ERYGAGGGDAVAFEVRTNPGADWGNLSAIASGGELARFALALKAALASRGDGDAAAPV
                     MIFDEVDQGVGGAVADAVGLRLKRLAERGQVIVVTHSPQVAARGDQHLKVAKYEQDGM
                     MRSAVAQLTPDETLEELARMLSGAEVTPEARAAAQRLKAVDAAAA"
     gene            1047..>2966
                     /gene="ligA"
                     /locus_tag="VF402_06465"
     CDS             1047..>2966
                     /gene="ligA"
                     /locus_tag="VF402_06465"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7799973.1"
                     /translation="MSPDDARALHAELAAQVQRHRDLYYNEETPELSDADYDALEARL
                     RALEDEFPDLKTADSPTAQVGAPISAKFAPVRHGAPMLSLDNAFASEDVGDFEARIRR
                     FLKLPDDEPVAFTAEPKIDGLSCSILYVNGELVRAATRGDGRTGEDITENVRTIGDIP
                     HRLHGEGWPNHIEVRGEVYMPLAEFAAMNARAEAEGAKVFANPRNAASGALRQLDANI
                     TASRPLHFFAYAWGEVIGGEVGGEFADTQWGALEAFRRWGFKVNPRSARVEGAQGLLD
                     VYDRLQTERAELGYDIDGVVYKVDRLDWQRRLGFVSRSPRWAVAHKFPAQQALTRLIA
                     VDIQVGRTGALTPVARLESVNVGGVVVTNVTLHNADEIARKDIHIGDMVRVQRAGDVI
                     PQVVGVELAERPADAKPYEFPAICPCPLKTEVVRGANIKGEEGVARRCSGDQACPHQH
                     VEYLKYVCSRRVLDIEGLGESNIEIFHRAGLLDEPADIYRLHERADRVRSAVLEERKR
                     QSAERQEKKGQAVDKALADEDRAFAGIDKLFAAIEDRRVLPLDRFIAALGVRHVGETT
                     ARLLARAYGSERHFHDAMTAAAGGDEAARHELEHHDQIGPVVAGTILSYFAHPYQRDI
                     YERFKAQVTVEDAEAV"
BASE COUNT          483 a          944 c         1112 g          427 t
ORIGIN      
        1 ccatgcgcgc acccgcgcca tgcaggcggg cgcgggttcg gacgatccgg tgctggcgcg
       61 gctgacggcg gcggcggacg cgctggaccg cacgctgatc gcggcggacg aggcgctggc
      121 cctgatggat caggcggcgg agagtctcga cgtcgagccg gggcaactgg atcgcgtcga
      181 ggagcggctg ttcgcgctgc gcgcgatggc gcgcaagctg ggtgtgctgg tcgaggatct
      241 gccgaaggaa cgcgcgcgca tcgcccggtc gctgaacgag atcgaggatc tggacgcgca
      301 tctgcgccgg ctcgatctgg cgatccatgc ggcggaaaag gatttccacg cggcggtcga
      361 agcgctgcgc gcgcgccgtc aggcggcggg cgcggcgctg agcgcggcgg tgatggcgga
      421 actgacgccg ctgaaactcg acaaggcgaa attccgcgtg caggtcgcgg cgctggagcc
      481 ggaacgctac ggcgcgggcg gcggcgacgc ggtcgcgttc gaggtgcgca ccaatccggg
      541 cgcggactgg ggcaatctga gcgccatcgc ctcgggcggc gaactggcgc ggttcgcgct
      601 ggcgctcaag gcggcgctgg cgtcgagagg cgacggagac gccgccgcgc cggtgatgat
      661 cttcgacgag gtcgatcagg gcgtcggcgg cgcggtcgcc gacgcggtcg ggctgcgcct
      721 gaaacggctg gccgaacgcg gtcaggtcat cgtcgtcacc cacagcccgc aggtggcggc
      781 gcgcggcgat cagcacctga aggtcgccaa gtacgaacag gacggcatga tgcgctcggc
      841 cgtcgcgcaa ctgacgccgg acgagacgct tgaggagctg gcgcgcatgt tgtcgggcgc
      901 cgaggtcacg ccggaagcgc gcgcggcggc gcagcggctc aaggctgtgg acgcggcggc
      961 ggcgtaggcc gttgacggtt gtcggcggcg cgggccttat gctacgccgg aaggattcca
     1021 caagaggaaa cgacgccttg gccagcctgt cccccgatga cgcccgcgct ttgcacgccg
     1081 aactggccgc gcaggttcag cgccatcgcg acctctatta taacgaggaa acgcccgaac
     1141 tgagcgacgc ggactatgac gcgctggagg cgcggctgcg cgcgctggag gacgaatttc
     1201 ccgatcttaa gacggcggac agtccgacgg cgcaggtcgg cgcgccgatc tcggccaagt
     1261 tcgcgccggt gcggcacggc gcgccgatgc tgtcgctcga caacgccttc gcgtccgaag
     1321 acgtcggcga tttcgaggcg cgcatccgtc gcttcctcaa actgcccgac gacgaaccgg
     1381 tcgccttcac cgccgaaccg aagatcgacg gcctgtcgtg ttcgatcctc tatgtgaacg
     1441 gcgagcttgt gcgcgcggcg acgcgcggcg acgggcgcac gggcgaggac atcaccgaga
     1501 acgtgcgcac catcggggac attccgcatc gtttgcatgg cgaaggctgg cccaaccata
     1561 tcgaggtgcg cggcgaggtc tatatgccgc tggccgaatt cgccgccatg aacgcgcgcg
     1621 ccgaggccga aggcgccaag gtgttcgcca atccgcgcaa cgccgcttcg ggcgcgctgc
     1681 ggcaactcga cgccaatatc accgcctcgc ggccgctgca tttcttcgcc tatgcgtggg
     1741 gagaagtgat tgggggcgag gtcggcggcg agttcgccga cacgcaatgg ggcgcgctgg
     1801 aggcgttccg gcgctggggg ttcaaggtca atccgcgctc ggcgcgggtc gagggcgcgc
     1861 aagggctgct cgatgtctat gaccggttgc agacggaacg cgccgaactg ggctacgaca
     1921 tcgacggcgt ggtgtacaag gtcgaccggc tcgactggca aaggcggctc ggcttcgtgt
     1981 cgcgctcgcc gcgctgggcc gtggcgcata agtttccggc gcagcaggcg ctgacgcggc
     2041 tgatcgccgt cgatattcag gtcgggcgca cgggcgcgct gacgccggtg gcgcggctgg
     2101 agtcggtcaa tgtcggcggc gtggtcgtca ccaacgtcac cctgcacaac gccgacgaga
     2161 tcgcgcgcaa ggacatccat atcggcgaca tggtgcgcgt gcagcgcgcg ggcgacgtga
     2221 tcccgcaggt cgtcggcgtc gaactggccg aacgtccggc ggacgccaag ccgtatgaat
     2281 tcccggccat ctgcccgtgc ccgctcaaaa ccgaggtcgt gcgcggcgcc aatatcaagg
     2341 gcgaggaggg cgtggcgcgc cgctgttccg gcgatcaggc ctgcccgcat cagcatgtcg
     2401 aatacctgaa atatgtgtgc agccgccgcg tgctggacat cgaggggctg ggcgaaagca
     2461 atatcgagat cttccaccgc gcgggcctgc tggacgaacc ggcggacatc taccgcctgc
     2521 atgagcgcgc cgatcgggtg cgctccgccg tgctggagga acgcaagcgc cagtcggcgg
     2581 agcggcagga aaagaagggg caggccgtgg acaaggcgct ggccgacgaa gaccgcgcct
     2641 tcgccgggat cgacaagctg ttcgccgcca tcgaggaccg gcgcgttctg ccgctggacc
     2701 gctttatcgc cgcgctgggc gtccggcatg tcggcgagac gaccgcgcgg ctgctggcgc
     2761 gcgcctatgg ctctgagcgg cattttcacg acgcgatgac ggcggcggcg ggcggcgacg
     2821 aggcggcgcg tcatgagttg gaacaccatg accagatagg gccggtggtc gccgggacga
     2881 tcctcagcta cttcgcgcac ccgtatcagc gcgacatcta tgaacgcttc aaggcgcagg
     2941 tgacggtcga ggacgccgag gcggtc
//
LOCUS       DASVLL010000391         4704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_79329, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000391 DASVLL010000000
VERSION     DASVLL010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79329"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VF402_06470"
     CDS             complement(<1..632)
                     /locus_tag="VF402_06470"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX7799974.1"
                     /translation="MKRLSTRLSALYGALFAFGLLMTALAGYLGVVGNARQTAQSELQ
                     AAARVFQRVWSDGAEVQAAGALLLAHDDGFRKALQNGDADTVGGDFAVLQKRIKADMG
                     FVIGADGQPMQGRGDPVAVAVAAALQGARDLHPRAGVLSFASRSYQVVAVPVLRAVGA
                     PERLGWIVFVSQLDAVEMQRLQALAPVPATASILTQTQGGLWMDAVGSERG"
     gene            898..1926
                     /locus_tag="VF402_06475"
     CDS             898..1926
                     /locus_tag="VF402_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX7799975.1"
                     /translation="MASFASDNAACAHPAVLAALAAANDGFAPAYGGDTVTDAMREAF
                     RAAFEAPDARVWPVFNGSACNGLALAAMIRPYESVLAHRVAHIHNDECGLPEFFTGGK
                     IVALDGADGKLTPDGVAELVAAALNHAPHTARPRALSLTQSTELGTVYTPDEVRALCE
                     TAHAHGLYVHMDGARLANAAAALGCRPAQITSEAGVDALSFGATKNGALAAEAVVIFN
                     PELARDFPYIHKRGGQLPSKARFISAQLLALLQDGLWLDLARRANRMAQALRDGLAAL
                     PGIRLLHPVEANALFVHLPRPVAERLTHAGHRFYPWPASGPDAYRLVTSFQTTDADVA
                     AFLRDAHV"
     gene            1919..2392
                     /gene="ybaK"
                     /locus_tag="VF402_06480"
     CDS             1919..2392
                     /gene="ybaK"
                     /locus_tag="VF402_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX7799976.1"
                     /translation="MSRTTPGTLCLSKLGVAFDLHPYAYDPDAPRVGLQAAQALGVDP
                     DQTFKTLMAEVDGRPVCVVVPSPREVSMKKLAAAFSGKSAHMMKVPDAERLTGYKVGG
                     VSPFGQRKPAPTVIDETAQLFERIYINAGQRGLLLSIAPDDAARVLNAPYVDLMA"
     gene            complement(2416..3189)
                     /locus_tag="VF402_06485"
     CDS             complement(2416..3189)
                     /locus_tag="VF402_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7799977.1"
                     /translation="MDKLPDDIRSDKAAILKEAQGLLKDPSRRRLLGNLLTLGGLSLA
                     TGVIVKNYHSVDEILRRISRFNDAAQGALFDPKRMAPEYSEADIANPFPFNAFYGLKQ
                     VPHVDAATYRLGLSGQIADKRPWTLEALRALPQTSQITRHICVEGWSAIGKWGGVRFS
                     DFLKQIGADTTCKYVGFKCADGYYTSIDMPTALHAQTLLTLSFRDHELPSWYGFPMKL
                     RMPTKLGYKNPKHIMDIFVTNVFPGGYWEDQGYNWFGGS"
     gene            complement(3193..3783)
                     /locus_tag="VF402_06490"
     CDS             complement(3193..3783)
                     /locus_tag="VF402_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7799978.1"
                     /translation="MDKKSIHPLWLRLIHAANAAAVVILILSGWRIYDATRFLGFGIP
                     ARYTLGGWLGGAIQWHFAAIWVVFAAALLYLILGLATRRIPRKFFPVSPRAFAGDLAA
                     ALRGRLGHEDLSVYNTVQRVAYLLAMLDVVVLIVSGLVLWKSVQFPYLRVLVGGYEGA
                     RYVHFAAMSFMAAFILVHVAMALLVPRTIKAMLWGR"
     gene            3922..4386
                     /locus_tag="VF402_06495"
     CDS             3922..4386
                     /locus_tag="VF402_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX7799979.1"
                     /translation="MPLSARRRFELINWYPPFIGAGIRVTGIDAGFTTVTVGMKLRWW
                     NKNIFGTQFGGALYMMCDPFFVAILMQNIGRDYIVWDKAASIRFLKPGRSDVRAVFHI
                     PPDEIARVKAEADARGKSEPEYTADIVDRDGTVIATVHKTLYVRRKAAAPPR"
     gene            complement(4393..>4704)
                     /locus_tag="VF402_06500"
     CDS             complement(4393..>4704)
                     /locus_tag="VF402_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEX7799980.1"
                     /translation="WLFTTPAVIFQPLSGLALARLAHYPMTAPWLIAALVLYVGVGAC
                     WAPVVGLQLRMRRLAARAAAEGGELPALYHRCFRLWFVLGCLAFPAVIAIFWLMAATP
                     G"
BASE COUNT          810 a         1612 c         1556 g          726 t
ORIGIN      
        1 ccgcgttcgc tcccgacggc gtccatccac agcccgccct gggtctgggt caggatcgag
       61 gccgtggccg ggaccggggc cagggcctgc aaacgctgca tttcgacggc gtccagttgc
      121 gagacgaaca cgatccagcc cagccgctcc ggcgcgccga cagccctgag caccggcacg
      181 gcgacgacct gataggagcg cgaggcgaag ctcagcaccc cggcgcgcgg atgcaggtcg
      241 cgcgcgccct gcaaggcggc ggcgaccgcg acggcgaccg gatcgccgcg cccctgcatc
      301 ggctggccgt cggcgccgat cacaaaaccc atatccgcct tgatgcgttt ttgcagcacg
      361 gcgaagtcgc cgccgaccgt atcggcgtcc ccgttttgca gcgccttgcg gaagccgtcg
      421 tcatgcgcca gcagcagcgc gcccgccgcc tgcacctcgg cgccgtcgct ccatacgcgc
      481 tgaaacacgc gcgcggcggc ctgcaactcg ctctgcgcgg tctgacgggc gttgccgacg
      541 acgcccagat aaccggccag cgcggtcatc agcagtccga aggcgaacaa tgcgccgtac
      601 agggccgata atcgggtgct gagccgtttc aagaccatga gacgccggaa atttctgtcg
      661 tatggccgga cgcccggacg ggcgggcctg accctttagg gtctgaacta tcacgcgatt
      721 tcataagacg gagttaaatg cgcgccgatg ctggcggcgg cgtcaaaatt tttccgccgg
      781 gttgactcat gtgtaaaaaa tcttcccggc ggcacacgtc tggacgcggc tccggcgggg
      841 cggtatgacg gccggtatga ccggacggcg tggtgggccg tccctttcgg agatctcatg
      901 gccagcttcg ccagcgacaa cgccgcctgc gcgcatcccg ccgtcttagc cgccctggcc
      961 gccgccaatg acgggtttgc gcccgcctat ggcggcgaca cggtcaccga cgcgatgcgc
     1021 gaagccttcc gcgccgcctt cgaggcgccg gacgcgcggg tctggccggt gttcaacggc
     1081 tcggcctgca acgggctggc gctggcggcg atgatccggc cgtatgaatc ggtgctggcg
     1141 caccgcgtcg cccatatcca taacgacgaa tgcggcctgc cggaattctt caccggcggc
     1201 aagatcgtcg cgctggacgg cgcggacggc aagctgacgc cggacggggt ggcggagctg
     1261 gtcgcggcgg cgctgaacca tgcgccgcat accgcgcggc cgcgcgcgct cagcctgacg
     1321 caatcgaccg aactggggac ggtctatacg ccggacgagg tccgcgccct gtgcgagacg
     1381 gcgcacgcgc acgggcttta cgtccatatg gacggcgcgc gcctcgccaa tgcggcggcg
     1441 gcgctcggct gccgtccggc gcagatcacg tccgaggccg gggtcgacgc gctgtccttc
     1501 ggcgccacca agaacggcgc cctggccgcc gaggcggtcg tgatcttcaa cccggagctg
     1561 gcgcgcgatt tcccttacat ccacaagcgc ggcgggcaat tgccgtccaa ggcgcgcttc
     1621 atctcggcgc agcttctggc gctgttgcag gacggcctgt ggctcgatct ggcgcgccgc
     1681 gccaaccgca tggcgcaggc gctgcgcgac ggtctggcgg ccctgccggg gatccgtctc
     1741 ctgcatccag tggaggcgaa cgcgctgttc gtccacctgc cccgcccggt cgccgagcgg
     1801 ctgacgcacg ccggtcaccg cttctatccg tggcccgcgt cggggccgga cgcctaccgg
     1861 ctggtgacca gcttccagac gacggacgcc gacgtcgcgg cctttctgag ggacgcgcat
     1921 gtctaggacc acgcccggca cgctgtgcct gtcgaagctc ggcgtcgcct tcgacctgca
     1981 cccttacgcc tacgacccgg acgcgccgcg cgtcggtttg caggcggcgc aggctttggg
     2041 cgtcgatccc gaccagacct tcaagaccct gatggccgag gtggacggca ggccggtgtg
     2101 cgtcgtcgtc ccgtcgccgc gggaggtgag catgaaaaag ctggcggcgg cgttttccgg
     2161 caagtcggcg catatgatga aggttcccga cgccgagcgc ctgaccggtt acaaggtcgg
     2221 cggcgtctcg cccttcggcc agcgcaaacc ggcgccgacc gttatcgacg aaaccgcgca
     2281 actgttcgaa cgcatctaca tcaatgcggg ccagcgcggc ctgcttttgt ccatcgcccc
     2341 cgacgacgcg gcgcgcgtgc tgaacgcgcc gtatgtcgat ctgatggctt aggggcggtg
     2401 gaggggtccg agcgcctaac tcccgccgaa ccagttatag ccctgatcct cccagtagcc
     2461 gccggggaag acgttggtga cgaagatgtc catgatgtgc ttgggattct tgtagcccag
     2521 cttggtcggc atgcgcagct tcatcggaaa gccgtaccag ctcggcagtt cgtgatcgcg
     2581 gaagctcagg gtcaaaagcg tctgggcgtg cagcgccgtc ggcatgtcga tcgaggtgta
     2641 atagccgtcg gcgcacttga agccgacata tttgcaggtc gtatcggcgc cgatctgctt
     2701 caggaagtcg gagaagcgca caccgcccca cttgccgatg gcgctccagc cctcgacgca
     2761 gatatggcgc gtgatctgcg aggtctgcgg cagggcgcgc agggcctcca gcgtccacgg
     2821 gcgcttgtcg gcgatctggc cggacaggcc cagccggtac gtcgccgcat cgacatgcgg
     2881 aacctgcttg aggccataga aggcgttgaa cggaaacgga ttggcgatgt cggcctcgct
     2941 gtattccggc gccatccgct tcggatcgaa cagcgcgccc tgcgcggcgt cgttgaagcg
     3001 cgaaatgcgg cgcaggatct catcgaccga gtggtaattc ttgacgatca ccccggtcgc
     3061 cagcgacagc ccgccgagcg tcagcagatt gcccagcagg cggcggcgcg acgggtcttt
     3121 gagcagccct tgcgcctctt tcaggatggc ggccttgtcg gatcggatgt cgtcgggaag
     3181 cttatccatg attcaccgcc cccacagcat ggccttgatc gtcctgggca caagcagcgc
     3241 catcgcgaca tggacgagga tgaacgccgc catgaagctc atggccgcga aatggacgta
     3301 acgcgcgcct tcgtaaccgc cgaccaacac gcgcaggtaa ggaaactgca ccgacttcca
     3361 cagcacgagg cccgagacga tcagcacgac cacgtccagc atggcgagca gataggcgac
     3421 gcgctgcacc gtgttataga cgctcaggtc ttcgtggccc agcctgccgc gcagggcggc
     3481 ggcgagatcg ccggcaaagg cgcgcgggct gacggggaag aacttgcgcg gaatgcgccg
     3541 cgtggccagg cccaggatca ggtacagaag cgccgccgcg aacaccaccc agatggcggc
     3601 gaaatgccac tggatcgccc cgcccagcca gccgcccagc gtgtagcgcg ccggtatgcc
     3661 gaagccgaga aagcgcgtgg cgtcatagat gcgccagccg ctcaggatca ggatgacgac
     3721 ggccgccgcg ttggccgcat ggatcagcct cagccacaaa ggatggatgc ttttcttgtc
     3781 cattcggcgc ctccctgccc cgttcaacct atcatgatac gcgcggcgcc gcatcccggt
     3841 tacagcccct tgccagatcg cgccgatgag cgcacaaagg tgacgttaag ggaaagattt
     3901 ggaacggaaa gattttgcgc catgcccctg tccgcccgcc gccgtttcga gctgatcaac
     3961 tggtatccgc cctttatcgg cgcgggcatc cgggtgaccg gcatcgacgc gggcttcacc
     4021 accgtcacgg tcgggatgaa gctgcgctgg tggaacaaaa acatcttcgg cacgcagttc
     4081 ggcggcgcgc tctacatgat gtgcgacccg ttcttcgtcg ccatcctgat gcagaacatc
     4141 ggccgcgact acatcgtctg ggacaaggcg gcctcgatcc gcttcctcaa gcccggccgc
     4201 tcggacgtgc gcgccgtctt tcacattccg ccggacgaga tcgcgcgcgt caaggccgag
     4261 gcggacgcgc gcggcaagtc cgaaccggaa tatacggcgg acatcgtcga ccgcgacgga
     4321 accgtcatcg ccacggtgca caagaccctc tatgtccgcc gcaaggccgc cgcgccgccg
     4381 cgatgacgcg cctcaacccg gcgtcgccgc catcagccag aagatggcga tcacggcggg
     4441 aaacgccagg cagcccagca cgaaccagag cctgaaacag cgatgataga gcgcgggcag
     4501 ttcgccgccc tcggcggcgg cccgcgccgc cagacggcgc atgcgcagtt gcagcccgac
     4561 caccggcgcc cagcaggcgc cgacgccgac atagaggacc agcgccgcga tcagccacgg
     4621 cgccgtcatc ggatagtgcg ccagccgcgc cagcgccagc ccgctcagcg gctggaagat
     4681 cacggcgggc gtggtgaaca gcca
//
LOCUS       DASVLL010000392         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_294433,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000392 DASVLL010000000
VERSION     DASVLL010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294433"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF402_06505"
     CDS             <1..249
                     /locus_tag="VF402_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX7799981.1"
                     /translation="YLGMIERLRPTDPVEKDASTLPQGDLPYSLVQAVIKLERAEALV
                     DILGPQFVTAYSRVKLHEYETFMRTISPWEREFLLLNV"
     gene            351..1628
                     /locus_tag="VF402_06510"
     CDS             351..1628
                     /locus_tag="VF402_06510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7799982.1"
                     /translation="MKNTGHPANSWYAETAPVVARPTLERDLDADVAIIGGGFTGLGA
                     ALELARHGVGAVVLEGAQIGSGASGRNGGQVHTGQRLDPEILTQKLGPDAARQLWDMA
                     QDARTSLRRFVRDYGVDCDWRDGLIHAWHRPGGEAEDRAYGDFIARRYGYEGFRLLSK
                     AETAAELGTGVYHGGSFDAGGGHLHPLKLALGLAAAAEAHGAKLYEHSRVRAWSPVAD
                     GVRLALANGARVRARTVLVCGNGYMEGLSERVDAHVMPINNFILTPEPLGDAAILPHD
                     YAAADSRFVVNYWRRTADNRLLFGGGETYTPWFPKDIKAFVRRNLLKIYPQLADAGIS
                     HAWGGTLGITLSRAPFVRRLGPNILVSAGYSGQGVVLAPYFGRLLARAVLGEARDLDL
                     LGRLPVPAFFGGRLLRWPTLAAGLSYYALRDRL"
     gene            1801..2865
                     /locus_tag="VF402_06515"
     CDS             1801..2865
                     /locus_tag="VF402_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7799983.1"
                     /translation="MTASPQASIPRSVPDSVSLSDIIAQTSRAKAVTAGKVRDIRQVT
                     SKLRILALNALIEAKHAGDAGRGFSVVADEVRGISGEVETLARDLGHEIVTLDALTQS
                     LATQSQGARLTDLALGAVEIIDRNLYERTCDVRWWATDAALVEQAAEPSPQRAAYACE
                     RLGVILDSYTVYIDLWLCDLDGRVIANGRPHQHRVIGADVRDRLWFSRALGLRSGQDF
                     AVADITTESLLGGAQIATYATGVRRGGKADGELIGILGIHFDWRPQAKAVVEGARLAE
                     DEKDRTRVLLIDAQGLVIAASDDKGVLSEHVRLRTEGRAQGHYVDSHSGAMIAFHRTP
                     GYETYRGLGWYGAIVQTAAE"
     gene            complement(2913..>3361)
                     /locus_tag="VF402_06520"
     CDS             complement(2913..>3361)
                     /locus_tag="VF402_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7799984.1"
                     /translation="MLEKGGDFVHGYTYSGHPTCAAVALANIEILERENLIERTRDVT
                     GPYLVKALKRLDAHPLIGETRSLGLIGAIEIVAEKGTNKRFKGAEGHAGPVVRDLCIK
                     NGLMVRAIRDSIVMCPPLIVSEAEIDRLVDILDRSLTEAEPALRAL"
BASE COUNT          527 a         1101 c         1173 g          560 t
ORIGIN      
        1 tatctgggca tgatcgagcg tttgaggccc accgatccgg tcgagaagga cgcctcgacc
       61 ctgccgcagg gcgacctgcc ctattcgctg gtgcaggcgg tgatcaagct ggagcgcgcc
      121 gaggcgctgg tcgacatcct ggggccgcag ttcgtcaccg cctattcgcg cgtcaagctg
      181 cacgaatacg agaccttcat gcgcaccatc agcccgtggg aacgggagtt cctgctgctg
      241 aatgtgtagc ctgagcgcac attgcccctc ccaccgcttc gcggtccccc ctccccgtaa
      301 acggggaggg ataaaatgac ctcatcctcc cccgtttacg ggggccttac atgaaaaaca
      361 ccggccaccc cgcaaactcc tggtacgccg aaaccgcgcc ggtcgtggcg cgtccgacgc
      421 tggagcgcga tctcgacgcc gacgtggcca tcatcggcgg cggcttcacc ggcctcggcg
      481 cggcgcttga gctggcgcgg cacggcgtcg gcgcggtcgt gctggagggc gcgcagatcg
      541 gttcgggcgc gtcgggccgc aacggcgggc aggtccatac cggccagagg ctcgatcccg
      601 aaatcctgac gcaaaagctc ggcccggacg cggcgcgcca gctttgggac atggcgcagg
      661 acgcccggac cagcctgagg cgcttcgtgc gcgactacgg cgtcgattgc gactggcgcg
      721 acggcctgat ccacgcatgg cacaggccgg gaggcgaggc cgaagaccgc gcctatggcg
      781 acttcatcgc gcgccgctac ggctatgagg gctttcgcct gctgtcgaaa gccgagaccg
      841 ccgccgaact cggcacgggc gtctatcacg gcgggtcgtt cgacgcgggc ggcggccatc
      901 tgcatccgct caagctggcg ctggggctgg ccgccgccgc cgaggcgcac ggcgcgaagc
      961 tctatgaaca cagccgcgtc cgcgcatggt cgccggtcgc ggacggcgtg cggctggcgc
     1021 tggcgaacgg cgcgcgggtc cgcgccagaa ccgtgctggt ctgcggcaac ggctatatgg
     1081 agggcctgag cgagcgcgtc gacgcgcacg tcatgccgat caacaacttc atcctgaccc
     1141 ccgaaccgct gggcgacgcc gcgatcctgc cgcacgacta cgcggcggcg gattcgcgct
     1201 tcgtggtcaa ttactggcgc aggacggcgg acaaccggct tttgttcggc ggcggcgaga
     1261 cctatacgcc gtggttcccc aaggacatca aggcgttcgt ccggcgcaac ctgctgaaaa
     1321 tctatccgca actggccgat gccgggatca gccatgcgtg gggcgggact ttgggcatca
     1381 cgctcagccg cgcgccgttc gtgcgccgtc tggggccgaa catcctcgtc agcgcgggct
     1441 acagcggtca gggggtcgtg ctggcgccct atttcggcag gctgctggcg cgggccgtgc
     1501 tgggcgaggc gcgcgatctc gacctgctgg gccgcctgcc cgtcccggcc tttttcggcg
     1561 gccgcctgct gcgctggccc accctggccg ccgggcttag ctactatgcg ttgcgcgaca
     1621 ggttataacg caactgcgaa tgaaggcgcg cgtcacgggc cttctgttac atggatgcga
     1681 cacctcaagg atcggcaggc gcggaaaggg tttttattct cccggcgccg gatcggttaa
     1741 gacgtggtta agccttgacg catgtaattt ttgctgcaac gcaaaagaag gtttccgacc
     1801 atgaccgcct ctccccaggc cagtatcccg cgtagcgtac ccgacagcgt ctccttgtcc
     1861 gacatcatcg cccagacctc gcgcgccaag gccgtgacgg cgggcaaggt gcgcgacatc
     1921 cggcaggtga cgtccaaatt gcgcatcctg gcgctcaacg cgctgatcga ggccaagcac
     1981 gccggcgacg ccgggcgcgg tttttcggtg gtggccgacg aggtgcgcgg catttccggc
     2041 gaggtcgaga ctctggcgcg cgatctcggc catgagatcg tcacgctcga cgccctgacg
     2101 cagtcgctgg cgacgcagag ccagggcgcg cgcctgaccg atctggcgct gggcgccgtc
     2161 gagatcatcg accgcaacct ttatgagcgc acctgcgacg tgcgctggtg ggcgaccgac
     2221 gcggcgctgg tcgagcaggc cgccgagccg tcgccgcagc gcgcggccta tgcgtgcgag
     2281 cggctggggg tcattctcga ttcctacacg gtctatatcg acctgtggct gtgcgatctc
     2341 gacgggcggg tcatcgccaa cgggcgtccg catcagcatc gggtgatcgg cgccgacgtg
     2401 cgtgatcgcc tgtggttctc gcgagcgctc ggtttgcgca gcggtcagga tttcgcggtc
     2461 gccgacatca ccaccgagag cctgctgggc ggggcgcaga tcgccaccta tgcgaccggg
     2521 gtgcggcgcg gcggcaaggc cgacggcgag ctgatcggca tactcggcat ccatttcgac
     2581 tggcggccgc aggcgaaggc cgtggtcgag ggcgcgcggc tggccgagga cgaaaaggac
     2641 cgcacgcgcg tgctgctcat cgacgcgcag gggctggtga tcgccgcttc ggacgacaag
     2701 ggcgtgctga gcgagcatgt gcggctgcgc acggaaggcc gggctcaggg ccactatgtc
     2761 gacagccaca gcggggcgat gatcgccttt caccgcacgc cgggctacga gacgtatcgg
     2821 gggctgggct ggtatggggc gatcgtgcag acggccgcgg aatagcgcgt cttcccctct
     2881 ccccgcttgc ggggagaggg gaaggttggc ggttacagcg cgcgcagcgc cggttcggcc
     2941 tcggtcaggg accggtcgag gatatcgacc agccggtcga tctcggcctc gctgacgatc
     3001 agcggcgggc acatgacgat ggagtcgcga atggcgcgca ccatcaggcc gtttttgatg
     3061 cacaggtcgc gcaccaccgg accggcgtgg ccttctgcgc ctttaaagcg tttattcgta
     3121 cccttctcgg ccacgatctc tatggcgccg atcaggccga ggctgcgcgt ttcgccgatc
     3181 aaagggtggg cgtcgaggcg cttcagggcc ttgaccagat aggggccggt gacgtcgcgg
     3241 gtgcgctcga tcaggttttc gcgctccagg atctcgatat tggccagcgc caccgccgcg
     3301 caggtcggat ggccggaata ggtgtagccg tggacgaaat cgccgccctt ttccagcatg
     3361 a
//
LOCUS       DASVLL010000393         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_400425,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000393 DASVLL010000000
VERSION     DASVLL010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400425"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..>1857
                     /locus_tag="VF402_06525"
     CDS             132..>1857
                     /locus_tag="VF402_06525"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7799985.1"
                     /translation="MSKHSGWISLLALAAASGAGAQTQSGDALLDAMGLNSQKAATET
                     HANLAPVKPLRGYTTYVMSAFNATSENKLSIFTSYDGKTYTSLASEVYTPQSGLLRDP
                     SILRARDGDYYVVYTTGWNGQSFGLAKSRDLRHWEHVRDVQLNLPGLANAKITNVWAP
                     EWFQDADGNVSIVVSLSEGGTKGPFSAYLIKANADLSDFSAPVAMQGLGGNHIDTFPI
                     KIGDRYWAITKNETTKTLERAWAASLTGPWTIDRTGDWAGWGDWIEGPALTPIKGADG
                     KDGWRLYFDDYRDKTYWYSDSFDNLDTWTKKQQLGGVSGAVRHFTVIREATDTVEAAT
                     RPTGTPEAVTWDRHSLMIGGKRVMIWAGEFQPFRLPSPSMWRDVLQKMKATGYNAVTL
                     YIDWGYHSSEEGHYDFSGVRNVERAIQMAEDEGLYVIIRPGPYVNAELSMGGFPGWVT
                     RQKAMARTDDPEYLKAVDEWQTQIDTIVARHQITNGGGKVIAFQIENELGDTSPSRQR
                     YMQHLADKVRADGISVPLFHNSASRLPNWTPRDSSAPWAVPGPTDLYAFDGYPGGGCT
                     GTRDPGKPN"
BASE COUNT          377 a          609 c          564 g          307 t
ORIGIN      
        1 gatcaatttt cgtcttccct atgcgtatat atcggccaat tttgattgat tttgatattt
       61 tccattgata tgccgccctg tcaatggcaa attcccgccg ccataagtca ataagacaaa
      121 gagggttcag ggtgtcgaaa cattcaggat ggatcagcct gctggcgctg gcggcggcgt
      181 ccggcgccgg cgcgcagacc cagagcggcg acgcgctgct cgacgccatg ggcctgaaca
      241 gccagaaggc cgccacggag acgcatgcga acctcgcccc ggtcaaacct ttgcgcggct
      301 atacgaccta tgtgatgtcg gccttcaacg ccacgtccga aaacaagctg tccatcttca
      361 cctcctatga cggcaagacc tacacctcgc tggcctcgga ggtttacacg ccgcaaagcg
      421 ggctgctgcg cgatccgtcg atcctgcgcg cccgcgacgg cgactattat gtcgtctata
      481 cgaccggctg gaacggccag agcttcggcc tcgccaaaag ccgcgacctg cgccattggg
      541 agcatgtcag ggacgtgcaa ctgaacctgc cgggcctcgc caatgccaaa attacaaacg
      601 tgtgggcgcc ggagtggttt caggacgccg acggcaacgt ctccatcgtc gtgtcgctga
      661 gcgaaggcgg gacgaagggg ccgttctcgg cgtatctgat caaggccaat gccgacctca
      721 gcgacttttc cgcgccggtc gccatgcagg gcttgggcgg caaccatatc gacactttcc
      781 cgattaaaat cggcgaccgc tactgggcga tcaccaagaa cgagacgacc aagacgctgg
      841 agcgcgcctg ggcggccagc ctgaccgggc cgtggaccat cgaccgcacc ggcgactggg
      901 cgggctgggg cgactggatc gaaggcccgg ccctgacgcc gatcaagggc gcggacggaa
      961 aagacggctg gcgcctctat ttcgacgact atcgcgacaa gacctactgg tattccgaca
     1021 gcttcgataa cctcgacacc tggacgaaga agcagcaact gggcggcgtc tccggcgcgg
     1081 tgcgtcactt caccgtcatc cgcgaagcga ccgatacggt cgaggccgcg accaggccca
     1141 cgggaacgcc cgaagcggtg acatgggacc gccattccct gatgatcggc ggcaagcggg
     1201 tgatgatctg ggcgggcgag ttccagccct tccgcctgcc ctcgccgtcc atgtggcgcg
     1261 acgtgctgca aaagatgaag gcgaccggct acaacgccgt caccctctat atcgactggg
     1321 gctatcattc atcggaagaa ggccattacg acttttccgg cgtgcgcaat gtcgagcgcg
     1381 cgatccagat ggccgaggac gaagggcttt acgtcatcat ccgtccgggg ccttacgtca
     1441 acgccgaact cagcatgggc ggcttccccg gctgggtgac gcggcaaaag gcgatggcgc
     1501 gcaccgacga tcccgaatac ctgaaagccg tcgacgaatg gcagacccag atcgacacga
     1561 tcgtcgcccg ccaccagatc accaatggcg gcggcaaggt catcgccttc cagatcgaga
     1621 acgaactggg cgacacctcg ccctcgcgcc agcgctatat gcagcatctg gccgacaagg
     1681 tgcgcgccga cggcatcagc gtgcccctgt tccacaattc ggccagccgc ctgcccaact
     1741 ggacgccgcg cgattccagc gcgccgtggg ccgtgccggg gccgaccgac ctctacgcct
     1801 tcgacggcta tccgggcggc ggctgcaccg gcacgcgcga tccgggcaag ccgaaca
//
LOCUS       DASVLL010000394         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_294527,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000394 DASVLL010000000
VERSION     DASVLL010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294527"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..560)
                     /locus_tag="VF402_06530"
     CDS             complement(171..560)
                     /locus_tag="VF402_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX7799986.1"
                     /translation="MTEPAPLRKPTEAELDILRVLWARGEASVRDVHAALGAERRTGY
                     TTVLKLMQIMTEKGLVTRREAGKAHLYQAAMRESDMQGRLLRDLSDKLFAGSTAMLAM
                     HALSLDAASDEELERIKALIARKRGQS"
     gene            complement(618..1547)
                     /locus_tag="VF402_06535"
     CDS             complement(618..1547)
                     /locus_tag="VF402_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799987.1"
                     /translation="MRRALVPLMLFAAVLSLMGLSLTACSRGARERQPGQAFLDSRIP
                     SGLAPQYYPPDGFVWSGYRADGLPEARYGVASPPVNPHAQVLIIADAGYPAETWFELT
                     RGLLAAGYGVWLLEPPGQGGAGRYLLQGNVIHSPDYRDADRAAAGLIRDLIHPTPQRP
                     LYVVGSGYGAITVLSLAAELKGPAYAGFTAYDPYLGGAIAPGGLWRHEDVAPGYWGAI
                     AQAWQMQNPDLRLRMKSESWRKQAMKAFSVVAGPHPTLRSGAPVTLFEPDTADAAQMK
                     AARALCAHTPDCRLRGSKGQQAFGGELLDALRS"
BASE COUNT          257 a          558 c          549 g          260 t
ORIGIN      
        1 cgccgccgtc atcgcgggca cggtcgccat cgccgccagc cccagccggt agcgcgcgcg
       61 gccgctcagc cgcgtcaggc acagatcgca cagccggtac agcgccgccg ccagcagcgc
      121 ctgccatccg gcgtgcatga gcgtcagtcc cagagcggtc ggaagcgtat tcatgattgg
      181 cctcgcttgc gcgcgatcag cgccttgatg cgttcgagtt cctcgtcgct ggccgcgtcg
      241 agcgacaggg cgtgcatggc cagcatggcg gtcgatccgg cgaacagctt gtcgctgaga
      301 tcgcgcagca accggccctg catgtcgctt tcgcgcatgg cggcctgata caggtgcgcc
      361 ttgcccgcct cgcgccgcgt caccagaccc ttttcggtca tgatctgcat cagtttcagc
      421 acggtcgtgt agccggtgcg gcgttcggcg cccagggccg catgcacgtc gcgcacgctg
      481 gcttcgccgc gcgcccacag gacgcgcagg atgtcgagtt cggcttcggt cggtttgcgc
      541 aggggggcgg gttcggtcat ggcgcaaatc tacgaacgct ttcgtatcga gtcaacgaaa
      601 atcttcgtag gcggcgttca agaccgcagc gcgtcgagca gttcgccgcc gaacgcctgt
      661 tggcccttgc tgccgcgcaa ccggcagtcc ggcgtatgcg cgcacagggc gcgggcggcc
      721 ttcatctgcg ccgcgtccgc cgtgtccggc tcgaacagcg tcaccggcgc gccggatctg
      781 agcgtcggat gcggccccgc gacgacggaa aacgccttca tggcctgctt gcgccagctt
      841 tcgcttttca tgcgcaagcg aaggtcggga ttctgcatct gccacgcctg cgcgatggcg
      901 ccccaatagc cgggcgcgac gtcttcgtga cgccacaacc cgcccggcgc gatcgcgccg
      961 ccgagatagg gatcgtaggc cgtaaagccc gcataggccg ggcctttcag ctccgccgcc
     1021 agcgacagga ccgtgatcgc gccgtagccg ctgccgacga cgtagagcgg gcgctgcggc
     1081 gtcgggtgga tcaggtcgcg gatcagaccg gcggcggcgc ggtccgcgtc gcgatagtcg
     1141 ggcgaatgga tgacattgcc ttgcagcagg tagcgcccgg cgccgccctg tcccggcggc
     1201 tccagcagcc agacgccgta acccgccgcc agaaggccgc gcgtcagctc gaaccaggtt
     1261 tccgccggat aaccggcgtc ggcgataatc agaacctgcg cgtgcggatt gaccggcgga
     1321 gaggccacgc cgtaacgcgc ctccggcagc ccgtccgcgc ggtagccgct ccagacgaag
     1381 ccgtccggcg gataatattg cggcgccagt cccgacggta tccggctgtc gagaaaggcc
     1441 tgacccggct ggcgctcgcg cgcgccgcgc gaacaggctg ttaagctcag gcccatcaga
     1501 ctcaaaacgg cggcgaacag catgaggggg acgagggcgc ggcgcatggc gggaatttaa
     1561 gcgagattcc ttcacgcgcc tatgccaatt cggcgcaaag cgttgccgat tgtgacttga
     1621 tctt
//
LOCUS       DASVLL010000395         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_400566,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000395 DASVLL010000000
VERSION     DASVLL010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400566"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF402_06540"
     CDS             complement(<1..317)
                     /locus_tag="VF402_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein"
                     /protein_id="HEX7799988.1"
                     /translation="MLVSTKSALKAAAAALILTSVAACASKPKPVATTPPPVAEQAPP
                     PAPPPTQQPVQQAPLPGIVPGSQQDFVVNVGDRIYFDTDQSSIRTDAQSVLAAQAAWL
                     TRYP"
     gene            complement(456..1727)
                     /gene="tolB"
                     /locus_tag="VF402_06545"
     CDS             complement(456..1727)
                     /gene="tolB"
                     /locus_tag="VF402_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX7799989.1"
                     /translation="MLTPGLAAAQGLTGTVDQGNIQPLPIAIEILGKDASGGDLGGQI
                     SKVVSADLDRSGYFAPIDPNAFIETALDVNVQPNWDSWKTINAQYLCSGRALVDADGR
                     LQVDFRLWDIFSQKQLLGQSLTATPENWRRIAHKIADAIYEKLTGQQGYFDTRIVFVA
                     ESGYRTARVRHLAIMDQDGFGASYLPVADGAKAVMTPRFSADSQEIAYMSLTADSARI
                     YIYNLETGRQEAVGQIHGMAFAPRFSPDGTKLAYSVASGGATNIYVMDLRTRATTRLT
                     GGGAINTSPCFSADGSQIAFTSDRGGSPQIYMMNADGSNVRRITFGAGIYSTPVFSPQ
                     GDKIAFTRQAGGRFSIGVMNPDGSGMKLLTSSYLEEGPTWAPNGRYIMFFRESPSGVS
                     SLWTVEVTGRVERQVPYFGGASDPAWSPRLK"
     gene            complement(1861..>2054)
                     /locus_tag="VF402_06550"
     CDS             complement(1861..>2054)
                     /locus_tag="VF402_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7799990.1"
                     /translation="TPNGRALQVNWTNKRNDPVWQAGASLAMAAVNKAAAESTDLPAN
                     IYGQKITFTFLAEKACEGR"
BASE COUNT          356 a          643 c          688 g          367 t
ORIGIN      
        1 ggatagcgcg tcagccacgc ggcctgcgcg gccagaacgc tctgggcgtc ggtgcggatc
       61 gaggactggt cggtgtcgaa atagatgcgg tcgccgacat tgacgacgaa gtcctgctgc
      121 gagccgggga cgatgcccgg cagcggcgcc tgctgcaccg gttgctgcgt gggcggcggc
      181 gcgggcggcg gagcctgctc ggccacgggc ggcggcgtcg tcgccacagg cttgggcttg
      241 ctggcgcagg cggccacgga ggtcagaata agcgcagcgg cggccgcttt cagggccgac
      301 ttcgtcgata ccagcatgtt tgtgtccctt tcaccaactt atttcaggca taatcgcacc
      361 agactacggc aagattatgc gcgttataaa ataaggaaac gattatatgc gtttttcggt
      421 ttgtcaaacg cataagcggt aattccatgg ccgccttatt tcagccgcgg cgaccaggcc
      481 gggtcggaag cgccgccgaa atagggaacc tgacgctcga cgcggccggt gacctcgacc
      541 gtccacagcg acgacacgcc gctgggcgac tcgcggaaga acatgatgta gcgcccgttc
      601 ggcgcccagg tcggcccctc ctccagatag gacgaggtca ggagcttcat gcccgaaccg
      661 tccgggttca tcaccccgat ggagaagcgt ccgcccgcct ggcgcgtgaa ggcgatcttg
      721 tcgccctgcg ggctgaacac cggcgtcgaa tagatgcccg cgccgaaggt gatgcggcgc
      781 acattggagc cgtcggcgtt catcatgtag atctgcggcg atccgccgcg atccgaggtg
      841 aaggcgatct gcgagccgtc cgccgagaag cacggcgagg tgttgatcgc cccgccgccg
      901 gtcagccgcg tcgtcgcgcg cgtgcgcagg tccatgacgt agatattggt ggcgccgccg
      961 ctggccacgc tgtaggccag cttggttccg tccggcgaga agcgcggcgc gaaggccatg
     1021 ccgtgaatct ggcccaccgc ctcctgacgg ccggtttcga gattgtagat gtagatgcgc
     1081 gccgaatccg ccgtcagcga catataggcg atttcctgcg aatccgccga gaagcgcggc
     1141 gtcatcaccg ccttggcccc gtcggccacc ggcaggtagg acgcgccgaa gccgtcctga
     1201 tccatgatgg ccagatggcg gacgcgcgcc gtgcgatagc cgctttcggc gacgaagacg
     1261 atgcgggtgt cgaaatagcc ctgctggccg gtcagcttct catagatggc gtcggcgatc
     1321 ttgtgcgcga tgcggcgcca gttttccggc gtggcggtca gcgactgccc cagcaactgc
     1381 ttctgcgaga aaatgtccca caggcggaaa tcgacctgca gccgcccgtc ggcgtccacc
     1441 agcgcgcggc ccgaacacag atattgcgcg ttgatggtct tccagctgtc ccagttcggc
     1501 tggacgttga cgtcgagcgc ggtctcgatg aaggcgttgg gatcgatcgg cgcgaaatag
     1561 ccggaacggt cgaggtccgc cgacaccacc ttggagatct ggccgcccag atcgccgccc
     1621 gaggcgtcct tgcccagaat ctcgatggcg atcggcagcg gctggatatt gccctgatcg
     1681 accgtgccgg tcagaccctg cgcggcggcc agacccggcg tcagcatcag gccggcgccc
     1741 agtccggcaa ggcccgtcag ggccagcgac cggcgcgtcg gttgcaaaat gtctggagtc
     1801 gtcttgatca tggcgttgct ccttgtagga taagcttccg gcaggtcggg cccaaggacg
     1861 ttaccgcccc tcgcaagcct tttccgccag gaaggtgaag gtaattttct gcccgtagat
     1921 attggcgggc aaatctgtcg attccgcggc ggccttattg acggccgcca tagcgagact
     1981 ggctccggcc tgccagaccg ggtcgttgcg cttattggtc caattgacct gaagcgcgcg
     2041 gccattggga gtca
//
LOCUS       DASVLL010000396         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_510300,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000396 DASVLL010000000
VERSION     DASVLL010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510300"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF402_06555"
     CDS             complement(<1..447)
                     /locus_tag="VF402_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7799991.1"
                     /translation="MTFADAWGDLRRDWADGWLSLICGVGVLLAFSQVWEAPLFGYNG
                     TPVSDGGLARAIYYPFYLAGLYLAALSWRRMAGAVWRAPLLLALLALCGLSYIWSIDP
                     SGTLRRCVALGMTMLAGYALAARFSWARLTETVAVAFVVMMLLSYVL"
     gene            complement(444..1169)
                     /locus_tag="VF402_06560"
     CDS             complement(444..1169)
                     /locus_tag="VF402_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7799992.1"
                     /translation="MVDLTREMAGLMTALGRPATARGRVLMFMAAYAGEGVSTVAREY
                     ARCEAAYAKKPVWLVDADLTGQGQLRAVGDDPARFGPPGALSKASPDGSAFFAISPTP
                     RGRDGAAIADARFIIAQPFLERRLWVTRFLREKLPPGARARLSDQPGYWRALRDHAQT
                     VVIDAPAFERGAAALKLAPLADGVIMVVSETDGDVQARLDLRHAIERAGGRVIGMVYN
                     RARHAKAGAPGKPAGLMGRAAAL"
     gene            complement(1269..2825)
                     /locus_tag="VF402_06565"
     CDS             complement(1269..2825)
                     /locus_tag="VF402_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7799993.1"
                     /translation="MGRNSVVWEERMRADEGWDEEEGGVVRLRPRAAAPKRGAALQLG
                     LGDILRLLLREAWLIVIVFCVIFGVGALVALKMPSNYTAGASLLMQLSQNYVYDPATG
                     DAARGAIATIDQVVQSEVEILNSTELKKRVIAKLGYKTILPDSPQYWAPKSDAQRQEA
                     DQAALKVLQGGFEASTAPQNNVVRLTFKHSDPMAAALILNTWIDEYQVYRRTVFKDTT
                     GPLLERQKQDFDAQLSVADRALQSFLAQHGVGDFQAAKTTYASVYDTVTKSLYDAQSQ
                     LATDRAKLAEINANLKSLSPEMSTERDLDLSVPNKIFALKQQRQELLSRYLPTAQPVK
                     DVDAQIAAYQAMKASGNGVGEQAHKMGVNPIYQDALTQKLNTEADIASLNGRIAQLQD
                     QAAQATAKMQALLGIEAQYNNLSADRDALQNNIKTFTQRIQENDAQQALSKEGLDDTV
                     RVVEKAAMPDKPRSLKRVILILSFLFAGFTALCAGLLRVYTRKGFASADMAARALDLP
                     VLAQAGMKSA"
     gene            2983..3696
                     /locus_tag="VF402_06570"
     CDS             2983..3696
                     /locus_tag="VF402_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7799994.1"
                     /translation="MLSRRHFLTLSLGVAGVLGTASRARADKPVKANEIAFANWTDEE
                     PLYLFYPGDKLEIDVAGAPELSRQTQVGPDGRVTLGLIGQVMAAFKSAPQLQDDIEKA
                     YQGVLRDPQVTVYPGETAPMRVLVGGEVKNAGWVDMTGDMDALQAVMAAGGFTHGAKT
                     HEVVLIRRSHDGRAMRRIIDLQKPLSGRSDRLEALRRFDIIYVPRTNIAEAGVFVEQW
                     INNLIPSPVLNYFTYRSFN"
     gene            3769..>3975
                     /locus_tag="VF402_06575"
     CDS             3769..>3975
                     /locus_tag="VF402_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7799995.1"
                     /translation="MSDLFLLSEAQMRRIEPFFPLSHGVPRVDDCRIISGIIYVIKHG
                     LMWRDAPKAYGPHKTIYNRFVRWSR"
BASE COUNT          664 a         1350 c         1288 g          673 t
ORIGIN      
        1 cagcacatag gacagcagca tcatgaccac gaaggcgacc gcgaccgtct cggtcagccg
       61 cgcccacgaa aaccgcgccg ccagcgcgta accggccagc atggtcatgc ccagcgccac
      121 gcagcgccgc aaggtgccgg acggatcgat ggaccagata taggacaggc cgcacagcgc
      181 cagcagcgcc agcagcagcg gcgcgcgcca gacggcgccc gccatgcgcc gccagctcag
      241 cgccgccaga taaagcccgg ccagatagaa gggataatag atcgcgcgcg ccagcccgcc
      301 gtcgctcacc ggcgtgccgt tatagccgaa cagcggcgct tcccagacct gcgagaaggc
      361 cagcagcacg cccacgccgc agatcagcga cagccagccg tccgcccagt cccggcgcag
      421 atcgccccag gcgtccgcga aggtcacagc gcggcggcgc ggcccatgag gccggccggc
      481 ttgcccggcg cgccggcctt ggcgtgccgc gcgcggttat agaccatgcc gatcacgcgt
      541 ccgcccgccc gttcgatggc gtgacgcagg tccagccgcg cctgcacgtc gccgtccgtc
      601 tcgctgacca ccatgatcac gccgtcggcc agcggcgcca gtttcagcgc cgccgcgccg
      661 cgctcgaacg cgggcgcgtc gatcaccacc gtctgggcgt gatcgcgcag ggcgcgccag
      721 tagccgggct ggtcgctgag ccgcgcgcgc gcccccggcg gcagcttttc gcgcaggaag
      781 cgcgtcaccc acaggcggcg ctccagaaag ggctgggcga tgatgaagcg cgcgtcggcg
      841 atcgccgcgc cgtcccggcc gcgcggcgtc ggcgagatgg cgaagaaggc cgaaccgtcg
      901 ggcgaggcct tgctgagcgc gcccggcggg ccgaagcgcg ccggatcgtc gccgaccgcg
      961 cgcaactggc cctgccccgt cagatcggcg tcgaccagcc agaccggctt tttcgcatag
     1021 gccgcctcgc agcgcgcata ttcgcgcgcc acggtcgaga ctccctcgcc cgcataggcc
     1081 gccatgaaca tcaggacgcg cccgcgcgcc gtcgcgggcc gccccagcgc cgtcatcagg
     1141 cctgccattt cacgggtaag gtcaaccatc ggcgcgttca gaacaatcct taaaactcgc
     1201 gcgactcacg ccgtccggcg aatcgcgcgc tcactgtcgc aaagcccggc gccgggttca
     1261 agccgccttc acgccgactt catcccggcc tgggccaata ccggcaggtc cagcgcgcgc
     1321 gccgccatgt cggcgctggc gaaccccttg cgcgtataga cccgcagcag ccccgcgcac
     1381 agggcggtga agcccgcgaa caggaaggac aggatcagga tcacccgttt caggctcctg
     1441 ggcttgtccg gcatggcggc cttctcgacc acgcgcaccg tgtcgtccag cccttccttc
     1501 gacagcgcct gctgcgcgtc gttttcctga atgcgctggg tgaaggtctt gatgttgttt
     1561 tgcagcgcgt cgcggtcggc gctgagattg ttgtactgcg cctcgatgcc cagcagcgcc
     1621 tgcatcttgg cggtcgcctg cgcggcctga tcctgaagct gggcgatgcg gccgttcagc
     1681 gaggcgatgt cggcctcggt gttcagcttc tgcgtcaggg cgtcctgata gatcgggttg
     1741 acgcccatct tgtgcgcctg ctcgccgacg ccattgccgg acgccttcat cgcctgatag
     1801 gcggcgatct gggcgtcgac gtccttgacc ggctgcgcgg tgggcaggta gcgcgacaga
     1861 agctcctgcc gctgctgctt cagcgcgaaa atcttgttcg gcacgctgag gtcgaggtcg
     1921 cgctcggtcg acatttccgg cgacagcgat ttgaggttgg cgttgatctc ggccagcttg
     1981 gcgcggtcgg tcgccagttg cgactgcgcg tcgtacagcg acttggtcac cgtgtcgtag
     2041 acgctggcgt aggtcgtctt ggcggcctgg aaatcgccga cgccgtgctg ggccagaaag
     2101 ctctgcaagg cgcggtcggc gacggacagc tgcgcgtcga aatcctgctt ctgccgttcc
     2161 agcagcggcc cggtcgtgtc cttgaacacg gtgcggcgat agacctgata ttcgtcgatc
     2221 caggtgttga ggatcagcgc cgccgccatc gggtcggaat gcttgaaggt caggcgcacg
     2281 acgttgttct gcggcgcggt cgaggcctcg aagccgcctt gcaacacctt gagcgcggcc
     2341 tgatcggctt cctggcgctg cgcgtcgctt ttcggcgccc agtattgcgg cgaatccggc
     2401 aggatggtct tgtagcccag cttggcaatc acgcgcttct tcaactcggt cgagttgagg
     2461 atttccactt ccgattgcac gacctgatcg atggtggcga tggcgccgcg cgccgcgtcg
     2521 ccggtggccg ggtcatagac gtaattctgc gacaactgca tcagaagact ggcgcccgcc
     2581 gtgtagttcg acggcatttt cagcgccaca agcgcgccga cgccgaaaat gacgcaaaag
     2641 acgatgacga tcagccaggc ttcgcgcagc aacaggcgca gaatgtcgcc cagccccagt
     2701 tgcagggccg cgccgcgctt cggcgccgcc gcgcgcggac gcaggcgcac gacgccgccc
     2761 tcctcctcgt cccagccttc atccgcccgc atgcgttctt cccacaccac tgaattccgg
     2821 cccatacccg cttcccatgc gcgtggactc gcgcaggaca cacgcatgga ttgcaaaccg
     2881 ggaatcgtct cgatcctcaa actagtccgg cgagcgttaa tgaatgttta ccattttagg
     2941 gtttgttaag gctgttccca agcctacaga atcggtcccg ccatgctgtc ccgcagacat
     3001 ttcctcaccc tgtccctcgg cgtcgcgggc gtcctgggga cggcgtcgcg cgcacgggcc
     3061 gacaagccgg tcaaggctaa cgagatcgcc ttcgccaact ggaccgacga ggagccgctc
     3121 tacctctttt atccgggcga caagctggag atcgacgtgg cgggcgcgcc ggagctgagc
     3181 cgccagaccc aggtcgggcc ggacgggcgc gtcaccctgg gcctgatcgg tcaggtcatg
     3241 gccgcgttca agagcgcgcc gcagcttcag gacgacatcg aaaaagccta tcagggcgtg
     3301 ctgcgcgacc cgcaggtcac ggtctatccg ggcgaaaccg cgccgatgcg cgttctggtc
     3361 ggcggcgagg tgaaaaacgc gggctgggtc gacatgaccg gcgacatgga cgcgctgcag
     3421 gcggtgatgg cggcgggcgg attcacccac ggcgccaaga cgcacgaggt ggtgctgatc
     3481 cggcgctcgc acgacgggcg cgccatgcgc cgcatcatcg atttgcaaaa accgctgtcg
     3541 ggccgctcgg accggctgga ggcgctgcgc cgcttcgaca tcatctacgt gccgcgcacc
     3601 aatatcgccg aggcgggcgt gttcgtcgaa caatggatca acaacctgat ccccagcccg
     3661 gtgctaaact acttcaccta ccggtcgttc aactaggctc aggacctatt aaatttgttg
     3721 tctaatcggc ttgaggctga ttcactcgat ggatggaggt gtgcagccat gagtgatctg
     3781 tttctgctga gcgaggcgca aatgcgtcgg atcgagccgt tctttccttt atcgcatggg
     3841 gttccgcggg ttgatgactg tcggatcatc tctgggatca tctatgtgat caagcacggt
     3901 ctgatgtggc gggatgcgcc gaaggcctat ggtccgcaca agacgatcta caaccggttt
     3961 gtgcgctgga gccga
//
LOCUS       DASVLL010000397         4111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_510413,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000397 DASVLL010000000
VERSION     DASVLL010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510413"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..449)
                     /locus_tag="VF402_06580"
     CDS             complement(78..449)
                     /locus_tag="VF402_06580"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX7799996.1"
                     /translation="MIEQLTDFPDTVVAIRLSGEITRTDYQDVFMPAVDAALKTHDKA
                     ALYYETAPGFTGYSLGAMGEDAVLGFRHYFRFSRVAIVSDVGWLRNAVAAFHPLFPCP
                     VRLYHPGEAAAAKRWIETGQM"
     gene            complement(720..4073)
                     /locus_tag="VF402_06585"
     CDS             complement(720..4073)
                     /locus_tag="VF402_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7799997.1"
                     /translation="MMFAKARAVALLSAVSGVALLASGQVWAQTGDTAGQTAGQTTSK
                     SDDNLTIVVTGQRKALETAEQRKKKTDQIVDSITAVDIGALPDRSVTEALQRVPGVTI
                     GRTDEPRDIDRLNVEGSGVMIRGLTWVRSEINGRDSFGAKNGRSLGWEDVTPELAAGV
                     DVYKNPSAEIVEGGLGGTINLRTWMPFDVKGDKLAFSADETYGDLRKKWSPSASVLVS
                     KRFDTKVGEMGLLLDVAHSDLYSRLNALEVDPYDAHSDNTVTSYDGGATTTTYSNALP
                     GQAAGSTVMVPVGVEYRLEDRDKTRDGLYGAFQWKPNDDTEFYATYFQTKSRLISQDH
                     FLQTSACCSATNNQSFMGGSPLMQVDDPDHPGSKKWVYDDSKAFTFDDDGNFVSGTIQ
                     DGAGGGDPVSNAMLFSLGTRYGDERAQTGDFSTGVKWRADRWDFKLDFQHIDSLHTDY
                     DMTVYNYLTAEGGVTLDLSGDLPKISMADTSNSAGDSVLYAAMDHHEIDKARQNTVTF
                     DANYDFASGFFKSFKFGYRATQRDSTVLDSGYNWGAIDAPWLFGGQTANAADYPENQQ
                     VVNFSNFFGGDVSVPALWMPTMNLAKSTSATTAYISKLFFTANTATGAPASGTYAYYC
                     ATCGNGTVPYGSYGGAYDPFPAGSLAGYMYVWAAATPNAATPSTGNFWSAFNGDYDYA
                     PTATSGVGTNLQSEKTQAVYGMLRFSQDSLFGASLPWDGNFGMRIVRTDSLSQGRGTI
                     VAMTANTNPSTMSEAAELATTFANGASNRTSGGNHYLNVLPSLNLRFKPTDNQFIRFA
                     VASSIVRPDFYQLQPSFTVYGSYQTRNATVADGVVDQTSGLAYTQTQLDDAAAAGTPV
                     TYNTGASFSFSTGNPNLKPMKALSYDLAYEWYMKPGSMLSVGLFDKELYNYIQSDQVS
                     IQMTNNGVSETVVGNMPQNHGHGQIRGVEAQSTYFYDFLPGWLSGFGTDVNFTILETH
                     GTQNTSASVYDSSQISSSKLKLPLEQLSRYSYNATLMYAKYGIDARLAYNWRSRYLMS
                     ASASNVVAPAYMEDYGQLDGSVMYALNKNFKIGIQAVNLTGARNIIDIDERDNWYYGT
                     EGNTSPSLIYRHNWTVADKRISLVLRGAF"
BASE COUNT          666 a         1218 c         1423 g          804 t
ORIGIN      
        1 ctggggctga cctataagta ttaaggcttc ctcccgtcca actgccgggc cgtccgcttt
       61 atgcgggcgg ctttttttta catctggccg gtttcgatcc agcgcttcgc cgccgccgcc
      121 tcgccggggt ggtagagccg caccgggcag gggaacagcg ggtgaaaggc ggcgacggcg
      181 ttgcgcagcc agccgacgtc gctgacgatc gcgacgcggg agaaccggaa atagtggcga
      241 aagcccagca cggcgtcctc gcccatggcg ccgaggctgt agccggtgaa gccgggcgcg
      301 gtctcgtaat agagcgccgc cttgtcgtgc gtcttcaggg cggcgtcgac cgcgggcatg
      361 aagacgtcct gatagtcggt cctggtgatc tcgccgctga ggcgtatcgc gacgaccgta
      421 tcggggaaat ccgtaagctg ttcgatcata gctctgtctc cggcatggct ctgtctccgg
      481 ccctcggccc ggcggcagtg tgcgcctgcg cccgccgccg cgcaaggggc gcaaaaaagc
      541 cggtcgcgag cgaccggcgt ggaagggatc gaacacaggc ttcgcggcgg cggcagccgc
      601 cgcgaagttt ttagagcgtt tccccaaaaa gtggttccac tttttggata aggaagagcg
      661 acaaaacaaa gacttagagc gccgatctga tgcaatcaga tcgaaaagcg ctctaaagct
      721 tagaacgcgc cgcgcagcac cagcgaaatg cgcttgtcgg cgaccgtcca gttgtggcgg
      781 tagatcagcg acggagaggt gttgccctcc gtgccgtaat accagttgtc gcgctcgtcg
      841 atgtcgatga tgttccgggc gccggtgagg ttgaccgcct ggatgccgat cttgaagttc
      901 ttgttcagcg cgtacatcac cgagccgtcg agctggccgt agtcctccat ataggcgggc
      961 gccacgacgt tggaggccga ggccgacatc aggtagcgcg agcgccagtt gtaggcgagg
     1021 cgcgcgtcga tgccgtactt ggcgtacatc agggtggcgt tgtacgaata gcgcgacaac
     1081 tgctccagcg gcagtttcag cttggacgag ctgatctggc tgctgtcata gacgctggcc
     1141 gaggtgttct gcgtgccgtg cgtctcaagg atggtgaagt tgacgtccgt gccgaagccg
     1201 ctcagccagc cgggcaggaa gtcgtagaaa taggtcgact gcgcctcgac gccgcggatc
     1261 tggccgtggc cgtggttctg cggcatgttg ccgaccacgg tttcggagac gccgttattg
     1321 gtcatctgga tgctgacctg atcgctctgg atgtagttgt aaagctcctt gtcgaacagg
     1381 ccgaccgaca gcatcgagcc gggcttcatg taccattcgt aggccagatc gtaggacagg
     1441 gccttcatcg gcttcaggtt cggattgccc gtgctgaacg agaagctggc gccggtattg
     1501 taggtgaccg gcgttccggc ggcggcggca tcgtccagtt gcgtctgcgt ataggccagc
     1561 ccgcttgtct ggtcgaccac gccgtcggcg acggtggcgt tgcgcgtctg atagctgccg
     1621 taaaccgtga acgagggctg caactgatag aagtccgggc gcacgatcga ggaggcgacc
     1681 gcgaagcgga tgaactggtt gtccgtcggt ttgaagcgca agttcaggct gggcagcacg
     1741 ttgaggtagt ggttgccgcc gctggtgcgg ttgctggcgc cgttggcgaa cgtggtggcc
     1801 agctcggcgg cctcggacat ggtcgacgga ttggtgttgg ccgtcatcgc cacgatggtg
     1861 ccgcgccctt gcgacagcga gtcggtgcgc acgatgcgca tgccgaaatt gccgtcccac
     1921 ggaagggacg cgccgaacag gctgtcctgc gagaagcgca gcatgccgta aacggcctgc
     1981 gtcttttcgc tttgcaggtt ggtcccgacg ccggaggtcg cggtcggggc gtagtcgtaa
     2041 tcgccgttga acgcggacca gaaattgccg gtggacggcg tcgccgcgtt cggcgtggcc
     2101 gcggcccaga catacatata gcccgccagg ctgcccgccg ggaacggatc gtaggcgccg
     2161 ccatagctgc cgtaaggcac ggttccgttg ccgcaggtcg cgcagtaata ggcataggtc
     2221 ccgctggcgg gcgcgcccgt cgccgtgttg gcggtaaaga acagcttgga aatataggcc
     2281 gtggtcgcgg acgtgctctt ggccaggttc atcgtcggca tccacagggc cggaaccgag
     2341 acgtcgccgc cgaagaagtt ggagaagttc acgacctgct ggttttcggg atagtcggcg
     2401 gcgttggcgg tctggccgcc gaacagccac ggcgcgtcga tggcgcccca gttatagccg
     2461 ctgtccagca cggtcgagtc gcgctgggtg gcgcggtagc cgaacttgaa cgacttgaag
     2521 aagccggagg cgaaatcgta attggcgtcg aaggtcaccg tgttctggcg cgccttgtcg
     2581 atctcgtggt ggtccatcgc cgcgtacagc accgaatcgc ccgccgaatt ggaggtgtcg
     2641 gccatgctga tcttcggcag gtcgcccgac aggtccagcg tgacgccgcc ttccgccgtc
     2701 aggtagttat agacggtcat gtcgtagtcg gtgtgcagcg aatcgatgtg ctggaagtcg
     2761 agtttgaagt cccagcggtc ggcgcgccac ttcacgccgg tcgagaagtc gccggtctgg
     2821 gcgcgttcgt cgccgtagcg cgtgccgagg ctgaacagca tggcgttgga caccggatcg
     2881 ccgccgcccg cgccgtcctg aatggtcccg ctgacgaaat tgccgtcgtc gtcgaaggtg
     2941 aaggccttgc tatcgtcata gacccacttt ttcgagccgg gatggtcggg atcgtccacc
     3001 tgcatgagcg gggagccgcc catgaagctc tggttgttgg tcgccgagca gcaggccgag
     3061 gtttgcagga aatggtcctg gctgatcagg cgcgacttgg tctggaagta ggtggcgtaa
     3121 aattcggtgt cgtcgttcgg cttccactga aaggcgccat agaggccgtc gcgcgtcttg
     3181 tcgcggtctt cgaggcggta ttcgacgccg accgggacca tgaccgtgct gccggcggcc
     3241 tgaccgggca gggcgttgct gtaggtcgtg gtggtcgcgc cgccgtcata ggaggtgacg
     3301 gtgttgtcgc tgtgcgcgtc gtaaggatcg acctccagcg cgttcaggcg gctgtagagg
     3361 tccgagtgcg cgacgtcgag cagcaggccc atctcgccga ccttggtgtc gaagcgcttg
     3421 gacaccagca ccgaggccga ggggctccac ttcttgcgca ggtcgccata ggtttcgtcc
     3481 gccgagaagg ccagcttgtc gcccttgacg tcgaacggca tccaggtgcg caggttgatc
     3541 gtgccgccga ggccgccttc gacgatctcc gccgacgggt tcttgtacac gtccacgccc
     3601 gccgccagtt ccggcgtcac gtcctcccag ccgagcgagc ggccgttctt ggcgccgaag
     3661 gagtcgcggc cgttgatttc cgaacgcacc caggtcaggc cgcggatcat cacgcccgag
     3721 ccttcgacgt tcagccggtc gatgtcgcgc ggctcgtccg tgcggccgat ggtgacgccc
     3781 ggcacgcgtt gcagcgcctc ggtcaccgag cggtcgggca gggcgccgat atcgacggcg
     3841 gtgatggaat cgacgatctg gtcggtcttc ttcttgcgct gctcggcggt ttccagcgcc
     3901 ttgcgctggc cggtgacgac gatggtcagg ttgtcgtcgg acttgctcgt cgtctggccc
     3961 gccgtctggc ccgccgtatc gccggtctgc gcccagacct gaccggaggc cagcagcgcc
     4021 acgccggaca cagccgacag cagggccacg gccctggctt tcgcgaacat catggattgt
     4081 ctcccttggt gtgttttttg gggtgtgttt t
//
LOCUS       DASVLL010000398         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_456118,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000398 DASVLL010000000
VERSION     DASVLL010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456118"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VF402_06590"
     CDS             <1..150
                     /locus_tag="VF402_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7799998.1"
                     /translation="SRNLIERMFCHLKDFRRIATRYDKKADNFLSAVLIAATFMWWIP
                     IESGP"
     gene            321..1175
                     /gene="bla"
                     /locus_tag="VF402_06595"
     CDS             321..1175
                     /gene="bla"
                     /locus_tag="VF402_06595"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HEX7799999.1"
                     /translation="MKLSMAVGAMLLALTLPAQASDADWTTSAAPFRIAGDIYYVGTK
                     GIASYLIVSNRQAILLDVGLEQNAPLVERNIAALGFKLSDVKIILNSHAHFDHAGGIA
                     RLKRDTGAKLYASPGDRWALEHGTHDGETTYPRGHFPPVGVDHVLKDGESVALGGARM
                     TATFTPGHTPGCTTWSTQAVEAGRTLNVVFPCSLSVAGNVLVGNRRYPGIVADYKRSF
                     AVLGAMKADIVLSNHPEIADVLGREARVRAGQGSAFIDRALLADIVSDARRAFDADLK
                     KQQQAKKR"
     gene            complement(1178..1879)
                     /locus_tag="VF402_06600"
     CDS             complement(1178..1879)
                     /locus_tag="VF402_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX7800000.1"
                     /translation="MHVTWPDIALRLFLSVVAGALIGLNRDINGKPAGMRTTILVAMA
                     ATLAMLQADMLLGTSGRATDSFAVLDLMRLPLGILSGIGFIGAGVILRHGGGVTGVTT
                     AASLWFVTVDGLCIGGGQIALAILALIVALAVLAMLKPLELRLPRWHVGDLWIGLAAD
                     GPAIAEIQARLTQGGVISQSEDIEYDATGRVTQVRLRVRFRARTAAADIPAVAGQLAA
                     LPGVGGVKWTFADKR"
     gene            complement(1882..3543)
                     /locus_tag="VF402_06605"
     CDS             complement(1882..3543)
                     /locus_tag="VF402_06605"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX7800001.1"
                     /translation="METLGLIDLAGFVALLLWGLHMVQNGVMDAYGPGIRRILQRGLK
                     HRLVAFLGGVGVTTVLQSSTATGLMAAGLAAAGTIDLASGLAVMLGANVGTTLIVQLL
                     SFDISVLTPILILAAALWHQVGRTSRQKDLARALIGLGLMLFALTHMTALLGHAARAE
                     GFDAVLKLVTQDLGLSLLLGAVLAWSMHSSIAVVLLVASFAQQGLAPLEASFALVLGA
                     NLGSAINPLLNMLAGKNPAALRLPAGNLANRVIGCLIGLPLLGLATHLPGLSEAAGRA
                     PANFHLVFNLVMALLFLPWLTPLARLLERCLPEALTADTGAPLYLDPALLRKPSLAIA
                     AAAREAMRMADVAEGMLVDVAAVFRKGGRNEAEAIHRTDDVLDALNRSIKIYLTALPA
                     NALTDNERRRCDQVLYAATTLEQVGDIIGNNLARRAGKLVRRQQAISPDGWQDMEPIF
                     QRVIGNVRLTAAVFMSGEKRALRTLMDEKEALEALEGKASRIHLDRLRQGSTDGLETS
                     ALEIDALHDLRQINDHLAAIAVSIWRAKAKKARQTARAAAAGAGS"
     gene            complement(3655..4059)
                     /locus_tag="VF402_06610"
     CDS             complement(3655..4059)
                     /locus_tag="VF402_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7800002.1"
                     /translation="MLSQKAKYAIRAMQELATLTAGETLQATELAERIRAPLHFLEGI
                     LMDLRREGLLRSRRGRSGGYALARPADMISIADLIRHVDGPLALTTCASRTAYARCDD
                     CPTPDDCRLRLVLLAARDAMAEVLEKSTLAQP"
BASE COUNT          793 a         1496 c         1385 g          719 t
ORIGIN      
        1 agccggaatc tgatcgaaag aatgttttgt catctcaaag acttcagacg catcgcgacc
       61 cgatacgaca aaaaggcaga caacttctta tctgccgttc ttatcgctgc caccttcatg
      121 tggtggatcc cgattgagtc cggaccctaa agcgttcccc aaaaagtgga gccacttttt
      181 ggataaggaa gagcggcaga tcgaaaagca ggccaaattt ttctcatggc tgctcccgaa
      241 taatctcgtt tgcgcctgcg tgcgtcccgt gaaaacctgc ggacgcacgc cggacggcgg
      301 cgcaatttcg ggagacggtt atgaaattat ctatggccgt cggcgccatg ctgctggcgc
      361 tgaccctgcc cgctcaggcg tccgacgccg actggacgac gtcggccgcg ccgttccgca
      421 tcgcgggcga catctattat gtcgggacca agggcatcgc ctcgtacctg atcgtctcga
      481 accggcaggc catcctgctc gacgtcgggc tggagcagaa cgcgccgctg gtggaaagga
      541 acatcgcggc gctgggcttc aaactcagcg acgtcaagat catcctcaac tcgcacgccc
      601 atttcgacca tgcgggcggc atagcccgcc tcaagcgcga caccggcgcg aagctctacg
      661 cttcgcccgg cgaccgctgg gcgctggagc acggaaccca cgacggcgag acgacctatc
      721 cgcgcggcca cttcccgccg gtcggcgtcg atcatgtgct gaaggacggc gagagcgtgg
      781 ctttgggcgg cgcgcgcatg acagcgacct tcacgcccgg ccatacccca ggctgcacca
      841 catggtcgac gcaggcggtc gaagcgggcc gcacgctgaa cgtcgtcttt ccgtgcagcc
      901 tgtcggtggc gggcaatgtg ctggtcggca accggcgcta tcccggcatc gtcgccgatt
      961 ataagcgcag cttcgccgtc ctcggcgcca tgaaggccga catcgtcctc agcaaccatc
     1021 ccgaaatcgc cgacgtgctg ggccgcgaag cccgcgtccg cgccggtcag ggatcggcct
     1081 tcatcgaccg cgctttgctg gcggacatcg tcagcgacgc gcgccgggcc ttcgacgcgg
     1141 acctcaaaaa gcagcagcag gcgaagaaac gctgatccta ccgcttgtcc gcgaacgtcc
     1201 atttgacgcc gccgacgccc ggcagcgcgg ccagttggcc cgcgaccgcc ggaatgtcgg
     1261 cggcggcggt tctggcgcgg aagcggaccc gcagccgcac ctgcgtcacg cggcccgtcg
     1321 cgtcgtattc gatatcctcc gactgcgata tgacgccgcc ttgcgtcagc cgcgcctgaa
     1381 tctccgcgat cgcgggcccg tcggcggcca gcccgatcca cagatccccg acatgccagc
     1441 gcggcaggcg cagctccagc ggcttcagca tggccagaac cgccagcgcg acgatcagcg
     1501 ccagaatcgc cagcgcgatc tgcccgccgc cgatgcaaag cccgtcgacg gtcacgaacc
     1561 acaggctggc tgcggtcgtg acgccggtga cgccgccgcc gtggcgcaga atgaccccgg
     1621 cgccgatgaa gccgatgccg ctcaaaatcc ccagcggcag acgcatcagg tcgaggaccg
     1681 cgaaggaatc cgtcgccctg cccgacgtgc cgagcagcat atcggcctgc aacatggcca
     1741 gggtcgccgc catcgcgacc agaatcgtcg tccgcatgcc cgcgggcttg ccgttgatgt
     1801 cccggttcag cccgatcaag gccccggcga cgacgctgag gaacagccgc agggcgatgt
     1861 ccggccatgt tacgtgcata ctcagctccc cgcgcctgcg gcggcggccc tcgccgtctg
     1921 ccgcgccttc ttggccttcg cgcgccagat cgagacggcg atcgcggcca gatggtcatt
     1981 gatctgacgc agatcgtgca gggcgtcaat ctccagcgcg ctggtttcga ggccgtccgt
     2041 cgatccctgc cgcaggcgat ccagatgaat ccgcgacgcc ttgccctcca gcgcctccag
     2101 cgcttccttc tcgtccatga gcgtcctgag cgcgcgcttt tccccgctca tgaagaccgc
     2161 cgccgtcaga cgcacattgc cgatgacgcg ctggaagatc ggctccatgt cctgccatcc
     2221 gtccggggaa atcgcctgct ggcggcgcac cagctttccc gcccggcggg cgaggttgtt
     2281 gccgatgatg tcgcccacct gttcgagcgt cgtcgccgcg tacagaacct gatcgcagcg
     2341 ccgccgctcg ttgtcggtca gtgcgttggc gggcagcgcc gtcagataga tcttgatgct
     2401 ccggttcagc gcgtccagca cgtcgtcggt ccggtgaatg gcctcggcct cgttccggcc
     2461 gcccttgcgg aacaccgccg cgacatcgac cagcatgcct tcggcgacgt ccgccatgcg
     2521 catcgcctcg cgcgccgccg ccgcgatggc gaggctgggt ttgcgcaaaa gcgcgggatc
     2581 gagatagagc ggcgcgccgg tgtcggcggt cagcgcctca ggcaggcaac gctccagaag
     2641 gcgggccagc ggcgtcagcc acggcaggaa cagcagcgcc atgacgaggt tgaagacgag
     2701 atggaaattg gccggcgccc ggcccgccgc ttcggacagg ccgggcagat gcgtggccag
     2761 acccagcagc ggcagcccga tcaggcagcc gatcacgcgg ttggccagat tgcccgcggg
     2821 cagccgcaag gccgccggat tcttgccggc cagcatgttc agcaacggat tgatcgcgct
     2881 gccgagattg gcgcccagca ccagcgcgaa actggcctcg agcggcgcca gaccttgctg
     2941 cgcgaaggag gcgaccaaaa ggacgaccgc gatgctggaa tgcatcgacc aggccagcac
     3001 ggcgcccaga aggaggctga ggccgagatc ctgcgtcacc agcttcagca ccgcgtcgaa
     3061 accctcggcg cgggcggcgt gacccagcag ggccgtcata tgggtcaggg cgaatagcat
     3121 caggccaagg ccgatcagcg cgcgggccag atccttttga cggctcgtcc gcccgacctg
     3181 atgccagagc gcggcggcca ggatgaggat cggcgtcagg accgagatgt cgaacgacaa
     3241 caattgcacg atcagcgtcg tgccgacatt ggcccccagc atgacggcca gcccggacgc
     3301 cagatcgatc gtccccgccg ccgccagacc cgccgccatc aggcccgtcg ccgtgctgct
     3361 ctgcaagacc gtcgtgacgc cgaccccgcc cagaaaggcg acgagccggt gtttcaggcc
     3421 gcgttgcagg atgcgcctta tgccgggtcc gtaggcgtcc atgacgccgt tttgaaccat
     3481 atgcagcccc cacaaaagga gcgcgacaaa accggccaga tcaatcagtc caagggtttc
     3541 catccgatgc aagcctcctt gcgaaccggt gaaaataagt ccgtgttcgg acgattacaa
     3601 atcaaaaatc cgcaagagcg gccctgaaag cggccctgag ccggaaacgg gcgttcaggg
     3661 ctgcgccagg gtcgattttt ccagcacctc ggccatggcg tcgcgcgcgg ccagcagcac
     3721 gaggcgcagg cggcaatcgt ccggcgtcgg gcagtcgtcg cagcgcgcat aggccgtgcg
     3781 gctggcgcag gtggtcaggg ccagcggccc gtcgacgtgg cggatgaggt cggcgatcga
     3841 gatcatgtcc gccgggcgcg ccagcgcata gccgccggag cgcccgcggc gcgagcgcag
     3901 caggccctcg cgccgcaggt ccatcaatat gccttcgaga aaatgcaggg gcgcgcggat
     3961 gcgctcggcc agctcggtgg cctgcaaggt ctcccccgcc gtcagggtcg ccagttcctg
     4021 catcgcgcga atggcgtatt ttgctttttg tgacaacatc cgcagtctgt ccgccctgag
     4081 cgcctctgtt cgcgagcctt agaattattc gccgcggacg tcaaacctaa tttgccgctg
     4141 ccggtctgcc gataagtatt tttcacctca cgcgttacca aaaatggcgc gattaattac
     4201 tacttattcg atatagtatt tggaggagtt tttatcatga ccgtgaccgt cagccatcct
     4261 cccctgttga tctggtacgc gcgctgtccg acgctgacgg cgctggggct ggtcgccaat
     4321 cgcggcgtgc tgcaacgcga gtttttacgt gaaaacgtcg ccctggtctc ggtgcgggaa
     4381 aattccacgc ccg
//
LOCUS       DASVLL010000399         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_295105,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000399 DASVLL010000000
VERSION     DASVLL010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295105"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF402_06615"
     CDS             complement(<1..402)
                     /locus_tag="VF402_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEX7800003.1"
                     /translation="MNYNTAVIDIGSNSVRLVIYRVEGRSIWPIFNEKVLAGLGRELK
                     TSGVLHKGGVKESLIALRRFKAILESYDLRDTHAVATAAVREAADGADFVARVATETG
                     FQVRVLSGAEEAWYSAQGVRCGHLDAHGVVGD"
     gene            complement(475..2742)
                     /locus_tag="VF402_06620"
     CDS             complement(475..2742)
                     /locus_tag="VF402_06620"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX7800004.1"
                     /translation="MTDSTATDLPPATETPLSALSEPVETKPASRQKVYDFSNDAPLD
                     PTLYVNRELSWLAFNQRVIEEAKNPRHPLLERLRFLSISANNLDEFYMVRVAGLQGQV
                     REGVRVLSADGHTPTEQLESVNRAAGQLMSRQQAIWTTIRNQLHDEGLIVLQPKDVNK
                     TDHAWLEKQFESQLFPVLTPLAIDPAHPFPFIPNLGFSIVLKLTRRRDARTMYALVPI
                     PTGVKRFWEIEAPARKPGETGPQRRKFISLENFVSLFLGQLFPGFDIVSRGLFRILRD
                     SDIELEEEAEDLVREFEALIKQRRLGSVVRVEIDASMPEDLRDFITGHLHAKAEDVVL
                     VGGILGMAEVSQLIPRDRNDLKFKPFEPRFPERIRDNGGDTFGAVREKDILVHHPFES
                     FDAVVQFLRQAARDPNVIAIKQTLYRTSKDSPIVAALIEAAEQGKNVTALVEIKARFD
                     EEANLRWARAMERAGVHVVYGFVEYKTHAKLSVVVRREGESLRTYCHFGTGNYHPITA
                     KIYTDLSLFTANPELGRDAMRVFNFITGYARPDKMEKLYFSPVTLKPGLIHLIDQEIQ
                     NAKAGKPAQIWAKLNALVDPSIIKKLYQASQAGVQIDLVIRGICCLRPGVAGVSDNIR
                     VKSIVGRFLEHARIVCFANGHAMPSDQARVFISSADWMSRNLDRRVEVMIPIENPTVH
                     RQVLDQIMVANLNDEAQSWSLGSDGVYHRIDTSHFDKPFSAHDYFMTNPSLSGRGRGV
                     KDLPQAFDHIGARKG"
     gene            complement(2797..3090)
                     /locus_tag="VF402_06625"
     CDS             complement(2797..3090)
                     /locus_tag="VF402_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800005.1"
                     /translation="MNLPDLASRIRAMRLVALPEPDEALLADILHKLFHERAISPSPD
                     LIDYLARRIDRSVAAAQKIVTDIEHYANGRPFNRALARAFLETRQGSFFDDEG"
BASE COUNT          572 a          911 c         1059 g          614 t
ORIGIN      
        1 atcgccgacc acgccgtgcg cgtcgagatg gccgcaacgc acgccctgcg ccgaatacca
       61 cgcctcttcg gcgccgctca gtacgcgcac ctgaaacccg gtctccgtgg cgacgcgcgc
      121 gacgaaatca gcgccgtcgg cggcttcgcg cacagcggcg gtggcgacgg catgggtgtc
      181 gcgcaggtcg taggattcga gaatcgcctt gaaacggcgc agcgcgatca ggctttcctt
      241 cacgccgccc ttgtgcagca cgccgctcgt tttcaactcg cggccgaggc ccgccagcac
      301 cttttcattg aagatcggcc agatcgaacg cccttcgacg cggtagatga ccagacgcac
      361 cgaattggag ccgatatcga tgacggcggt gttgtagttc aagaagccag cctccggtcg
      421 cttgcctttc gcttaaagcg tttgcggcgg tttgaccagt tcaaaagggc gttatcatcc
      481 cttgcgggcg ccgatatgat cgaaggcctg cggcagatcc ttgacgccgc gcccgcgccc
      541 ggaaagcgac gggttggtca tgaaataatc gtgcgcggag aagggcttgt cgaaatggct
      601 ggtgtcgatg cggtgataga cgccgtcgct gcccagcgac cagctctgcg cctcgtcgtt
      661 gagattggcg accatgatct gatccagcac ctgacgatga acggtcgggt tctcgatcgg
      721 gatcatcacc tcgacgcggc ggtcgagatt gcggctcatc cagtcggcgg acgagatgaa
      781 gacgcgcgcc tgatcggacg gcatggcgtg gccgttggcg aagcagacga tgcgcgcatg
      841 ttcgaggaag cgcccgacga tcgacttgac gcggatattg tccgacacgc ccgccacgcc
      901 cggacgcagg cagcatatgc cgcggatgac gagatcgatc tgcacgcccg cctgcgacgc
      961 ctgatacagc ttcttgatga tgctcggatc gaccagcgcg ttgagcttgg cccatatctg
     1021 cgcgggtttt ccggcctttg cgttctgaat ttcctgatcg atcagatgga taaggccggg
     1081 ttttaaggtg acgggcgaga agtagagctt ctccatcttg tccggccgcg catagccggt
     1141 gatgaagttg aacacgcgca tggcgtcgcg gcccagttcg gggttggcgg tgaacagcga
     1201 caggtcggta tagatcttgg ccgtgatcgg gtgatagttg ccggtgccga aatggcagta
     1261 ggtgcgcagc gattcgccct cgcgccgcac cacgacgctg agcttggcgt gggtcttgta
     1321 ttcgacgaag ccgtaaacga catgcacgcc cgcgcgctcc atggcgcgcg cccagcgcag
     1381 gttggcctcc tcgtcgaagc gcgccttgat ctcgaccagg gcggtgacgt tcttgccctg
     1441 ctcggcggcc tcgatcaggg cggcgacgat gggggaatcc ttggaggtgc ggtaaagcgt
     1501 ctgcttgatg gcgatgacgt tcggatcgcg cgcggcctga cgcaggaact gcaccacggc
     1561 gtcgaagctt tcgaacgggt ggtggaccag aatgtccttt tcgcgcacgg cgccgaaggt
     1621 gtcgccgccg ttgtcgcgga tgcgttcggg aaagcgcggc tcgaaaggct tgaatttcag
     1681 gtcgttgcgg tcgcgcggga tcagttgcga cacctcggcc atgccgagaa tgccgccgac
     1741 cagcaccacg tcctcggcct tggcgtgcag atggccggtg atgaagtcgc gtaagtcctc
     1801 gggcatggag gcgtcgatct cgacgcgcac gaccgagccg aggcggcgct gcttgatcag
     1861 cgcctcgaat tcgcgcacca gatcctcggc ctcttcctca agctcgatgt cggaatcgcg
     1921 caggatgcgg aacaggccgc gcgacacgat gtcgaagccg gggaaaagct ggcccagaaa
     1981 caggctgacg aagttttcca gcgagatgaa tttgcgccgc tgcggcccgg tttcgcccgg
     2041 tttgcgcgcc ggggcctcga tctcccagaa gcgcttgacg ccggtgggga tcggaaccag
     2101 cgcgtacatg gtcctggcgt cgcggcgccg ggtcagcttc agcacgatcg agaagccgag
     2161 attgggaatg aacggaaacg ggtgcgccgg gtcgatggcc agcggcgtca gcaccgggaa
     2221 aagctggctc tcgaactgtt tttccagcca cgcatggtcg gtcttgttga cgtccttcgg
     2281 ttgcaggacg atcaggccct cgtcgtgcaa ctgattgcgg atcgtggtcc agatcgcctg
     2341 ctggcggctc atgagctggc cggcggcgcg gttgaccgat tcgagttgtt cggtgggcgt
     2401 atggccgtcg gcgctcagga cgcgcacgcc ttcgcgcacc tgaccttgca ggcccgcgac
     2461 gcgcaccatg tagaattcgt cgaggttgtt ggccgagatc gacaggaagc gtaagcgttc
     2521 cagcagcgga tggcgcggat tcttcgcctc ctcgatgacg cgctggttga aggccagcca
     2581 gctcagttcg cggttgacgt agagcgtcgg gtcgagcggc gcgtcgttgc tgaaatcata
     2641 gaccttttgc cgcgacgcag gtttggtttc gaccggctcg gacagggcgg acaaaggcgt
     2701 ttcggtggcg ggtggcaggt ctgtggcggt cgaatcggtc atgaaatcag ctatgggcgt
     2761 tttaaagaaa aagactacgc ccgatttgtt taaaaactag ccttcatcgt cgaaaaacga
     2821 accctggcgg gtttcgagga aggcgcgggc cagcgcccgg ttgaacggac gtccgttggc
     2881 atagtgctcg atgtctgtga caattttttg cgccgccgcc acggaacggt cgatgcggcg
     2941 cgccagatag tcgatcaggt cgggcgaggg cgatatggcg cgctcatgga acagcttgtg
     3001 caggatgtcg gccagcagcg cctcgtcagg ctccggcagg gcgacgagac gcatggcgcg
     3061 gatgcgcgag gccagatcgg gcaggttcaa atcccacaga ataggctgta tttttgtcgt
     3121 taaaacaaga taatgtcctg ttttctgaac gtgatt
//
LOCUS       DASVLL010000400         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_456722,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000400 DASVLL010000000
VERSION     DASVLL010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456722"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VF402_06630"
     CDS             <1..635
                     /locus_tag="VF402_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7800006.1"
                     /translation="LNRQGVTCFVLFYRLAADGWDRPAYVGNADAQRAMRVIRSRAAG
                     LKIDPARLGVAGFSAGGFLTASLATRHGADFYAPVDAADRQSARPFLAAPIYPVQSVD
                     PAYAYPGLITTLFGGHVTPEIVKTWSPDRNVGPDTAPCFLCQNEDDQTVPVRNTVALR
                     DALAAAKIPVETHLFARGGHGFGMKDDPAQPWHIWPQLLANFARSRGLMG"
     gene            722..3133
                     /locus_tag="VF402_06635"
     CDS             722..3133
                     /locus_tag="VF402_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7800007.1"
                     /translation="MALATLAYSAAGGAMALALSTTWLALRLRARLTAAQSGLRDDLH
                     HKQGLLRELDSATQAFDEAFLAVEGDTVRPVWGEDSLRACAEALGLRNLDKNADGDLA
                     PAVMAALGEVSAEAADGLKALISEGRPCRFEIFTGGDAEDVNAPPGMTLIVEGRASGA
                     TAWIRLALAGARVSLSSGPFAQMAERLPAPCWISARDGRLVWANAAWLRAAEAPSLEA
                     AIKNNLTPDRNADALVMEAQAARTRRDGFRWLSLNGQRRAFQIIAEPLNDAYVCAYML
                     DVTEAEESREALRRHAKAHDETLDALEDAVAIFGPERQLTFHNRAFERLWELEPAWLA
                     ERPTHGEWLDRLRQKRRLPETSDYAAFKARESEAFSLTHTGDDEMWSLPDGRSLRVVH
                     QPHPLGGLLLLFSDKTGELKLKAQFNALIQVQKSTLDQLNDAVSVYGSDGRLRLRNSA
                     FDRFWNIRSDDMAAVMDFAEVAELCLPLVHDRGFWAELKARVTDTDPLARAPQKGEVK
                     VSDGRLAQWRTQPLPDGATLVAFSDITATRQLEKAIEDRDEALNESVRLKRDFVANVS
                     YELRTPLTTIVGYADLLQAQAGDNPKQKAYLDSIQAAAQELARSIDDVLDMAQIDAGE
                     MSIARAPVRLLDLVAEVGARLADSVKARGVSFDYRGVIETGEINVDARRLAQCLEHLL
                     GNAIRNAAQGGSVTLNARRNGDTLELEVAYTGRGIPYHVQAHIFDRYVGRERGGPGLG
                     LALVKALVELHDGWITLESEPDKGASFKIHLPGQSAIQSSAAVQVVEDKSAAAVDDDA
                     VRGVA"
     gene            3250..>3744
                     /locus_tag="VF402_06640"
     CDS             3250..>3744
                     /locus_tag="VF402_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX7800008.1"
                     /translation="MPKDLRRLRPVISRWMSLRHWRSQLVFWSGGLLVGLVAVMMAIV
                     ADKAQALFGVVRAHIFWLPLFLTPMGFAASAWVTKRWFGGAQGSGIPQVIAASEIVRH
                     NPEKAQNLVSMRIVIGKIAMLMLGLVCGAPIGREGPTVQVGAALMRMTGRIGGIVQNE
                     SALLL"
BASE COUNT          572 a         1284 c         1305 g          583 t
ORIGIN      
        1 tcctcaaccg tcagggcgtg acctgcttcg tgctgttcta ccggctggcc gccgacggct
       61 gggacaggcc cgcctatgtc ggcaacgccg acgcgcagcg cgccatgcgc gtgatccgtt
      121 cgcgcgccgc cggactgaag atcgatccgg cgcgcctcgg cgtcgcgggc ttttcggcgg
      181 gcggcttcct gacggccagc ctcgccacgc gccacggcgc cgacttctat gcgccggtgg
      241 acgccgccga ccggcaatcg gccaggccgt tcttggccgc gcccatctat ccggtgcaga
      301 gcgtcgaccc ggcctacgcc tatccgggcc tgatcacgac cctgttcggc ggccatgtga
      361 cgccggagat cgtcaagacc tggtcgccgg accgcaatgt cgggccggac acggcgccgt
      421 gcttcctgtg tcagaacgag gacgaccaga ccgtgccggt gcggaatacc gtggccctgc
      481 gcgacgcgct ggcggcggcg aagattccgg tcgagacgca cctgttcgcc cgcggcggcc
      541 acggcttcgg catgaaggac gatcccgccc agccctggca tatctggccg caactgctgg
      601 ccaatttcgc ccgcagccgc ggcctgatgg gctgagcggc gcggcggcgt tctttttaca
      661 cgcgaatcaa cttgctctat agttaaggct tctgtgattc gtatggatgt aggcgcgccg
      721 catggccctt gcgaccctgg cttattccgc agcgggcgga gcgatggcat tggccctgtc
      781 gacgacatgg ctggccttgc ggctgcgcgc gcgcctgacc gccgcgcaaa gcggcctgcg
      841 cgacgatctg caccataagc aaggcctgct gcgcgaactg gattcggcca cgcaagcctt
      901 cgacgaggcg tttctggccg tcgagggcga cacggtgcga ccggtctggg gcgaggactc
      961 tttgcgcgcc tgcgccgaag cgctgggcct caggaattta gacaaaaacg ccgacggcga
     1021 tctggccccc gccgtcatgg cggcgctggg cgaggtgtcg gccgaggccg ccgacggact
     1081 gaaggcgctg atttccgaag gccgtccgtg ccgcttcgag atcttcaccg gcggcgacgc
     1141 cgaggacgtc aacgcgccgc cgggcatgac gctgatcgtc gagggccgcg cgtccggcgc
     1201 gacggcgtgg atccggctgg ccctggccgg ggcgcgcgtt tcgctgtcgt cgggaccgtt
     1261 cgcgcagatg gccgagcgcc tgcccgcgcc gtgctggatc agcgcgcgcg acgggcgtct
     1321 ggtctgggcc aacgccgcct ggctgcgcgc cgccgaggcg ccgtcgctgg aggcggcgat
     1381 caagaacaac ctgacgccgg accgcaacgc cgacgcgctg gtcatggagg cgcaggccgc
     1441 gcgcacgcgc cgcgacggct tccgctggct cagcctgaac ggccagcgcc gcgcgtttca
     1501 gatcatcgcc gaaccgctca acgacgccta tgtctgcgcc tacatgctgg acgtgaccga
     1561 ggccgaggag agccgcgagg cgctgcgccg ccacgccaag gcgcacgacg agacgctgga
     1621 tgcgctggag gacgcggtgg cgatcttcgg cccggaacgg caactgacct tccacaaccg
     1681 cgccttcgag cgattgtggg agctggagcc cgcctggctg gccgaacgcc cgacgcacgg
     1741 cgaatggctg gaccggctgc gccagaagcg ccgcctgccc gaaacctccg actacgccgc
     1801 cttcaaggcc cgtgagagcg aggcgttcag cctgacccac accggcgacg acgagatgtg
     1861 gagcctgccc gacgggcgtt ccttgcgcgt cgtgcatcag ccgcacccct tgggcggcct
     1921 gttgctgctg ttctcggaca agacgggcga gctgaagctg aaggcgcagt tcaacgccct
     1981 gattcaggtg cagaaatcga cgctggacca attgaacgac gcggtgtcgg tctatggctc
     2041 ggacggacgg ctgcgcctgc gcaattcggc cttcgaccgc ttctggaaca tccgttccga
     2101 cgacatggcc gccgtgatgg acttcgccga ggtcgccgag ctgtgcctgc cgctggtcca
     2161 cgaccgcggc ttctgggccg agttgaaggc gcgcgtcacc gacaccgacc cgctggcgcg
     2221 cgcgccgcag aagggcgagg tcaaggtgtc cgacgggcgt ctggcgcaat ggcgcaccca
     2281 gccgctgccg gacggcgcca cgctggtcgc cttttccgac atcaccgcca cgcggcaact
     2341 ggaaaaggcc atcgaggacc gcgacgaggc gctcaacgaa tcggtgcggc tcaagcgcga
     2401 tttcgtcgcc aacgtctcct acgaactgcg cacgccgctg accaccatcg tcggctacgc
     2461 cgacctgttg caggcgcagg cgggcgacaa tccgaaacag aaagcctatc tcgactcgat
     2521 tcaggcggcg gcgcaggaac tggcgcgctc gatcgacgac gtgctggaca tggcccagat
     2581 cgacgcgggc gaaatgtcga tcgcgcgcgc gccggtgcgg ctgctcgatc tggtcgccga
     2641 ggtcggggcg cggctggccg acagcgtcaa ggcgcgcggc gtcagcttcg attaccgggg
     2701 cgtgatcgag accggcgaga ttaatgtcga cgcccggcgg ctggcgcaat gcctcgaaca
     2761 cctgctgggc aacgccatcc gcaacgcggc gcagggcggt tcggtcacgc tgaacgcgcg
     2821 ccgcaacggc gacacgctgg agcttgaggt cgcctatacc gggcgcggca tcccctatca
     2881 cgtgcaggcg cacatcttcg accgctatgt cgggcgcgag cgcggcgggc cggggctggg
     2941 gctggccctg gtcaaggcgc tggtcgagct gcacgacggc tggatcacgc tggagtccga
     3001 gccggacaag ggcgccagct tcaaaatcca cctgccgggc cagagcgcga tccagtcctc
     3061 cgccgccgtt caggtcgtcg aggacaagag cgccgccgcc gtggacgacg atgcggtgcg
     3121 cggcgtcgcc tgaacgcacg cggcgcgttt gcggggattt gcgaccggat gcggccgttt
     3181 caggcttgat ccggtggata agatagccga tagcgttccg ccccgacctc cctggagacc
     3241 gcccgtccca tgcccaagga tcttcgccgc cttcgtcccg tcatcagccg ctggatgagc
     3301 ctgcggcact ggcgctcgca actggtgttc tggagcggcg gcctgctggt cgggctggtg
     3361 gccgtgatga tggccatcgt cgccgacaag gcgcaggccc tgttcggcgt cgtgcgcgcg
     3421 catatcttct ggctgcccct gttcctgacg ccgatgggct tcgcggcctc ggcctgggtg
     3481 acgaaacgct ggttcggcgg cgcgcagggc tccggcattc cgcaggtcat cgccgccagc
     3541 gagatcgtgc gccacaaccc cgaaaaggcg cagaacctcg tctcgatgcg catcgtgatc
     3601 ggcaagatcg ccatgctgat gctgggtctg gtctgcggcg cgccgatcgg gcgcgaaggc
     3661 cccaccgttc aggtcggggc ggcgctgatg cgcatgaccg gccgcatcgg cggcatcgtg
     3721 cagaacgaat cggcgctgct gctg
//
LOCUS       DASVLL010000401         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_25860, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000401 DASVLL010000000
VERSION     DASVLL010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25860"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VF402_06645"
     CDS             complement(<1..852)
                     /locus_tag="VF402_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7800009.1"
                     /translation="MSEFNPSRDDFAALLDESMHGRDMLEGQVIHGVVVGVEKDYVTV
                     DVGLKTEGRIPLKEFGSEGANLKTGDHVEVYLERVENAMGEAVISRDKARREEAWTRL
                     EAIFNRGEPVMGSIVGRVKGGFTVDMDGASGFLPGSQVDIRPVRDVTPLMGKEQPFAI
                     LKMDRPRGNIVVSRRAILEEARAEQRTELVGQLAEGEIREGVVKNITDYGAFVDLGGI
                     DGLLHVTDMSWKRVSHPSQVLNVGDTVKVQIIKINPDTQRISLGMKQLQSDPWENVDV
                     KYPVGGKF"
     gene            complement(1096..1731)
                     /gene="cmk"
                     /locus_tag="VF402_06650"
     CDS             complement(1096..1731)
                     /gene="cmk"
                     /locus_tag="VF402_06650"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7800010.1"
                     /translation="MKTPFIIAVDGPAASGKGTLAAILAADYGLPCLDTGLLYRAVGH
                     LARKADPDDHAAIAALAQAIDADLLADAALRGRMAGEMASRVAAIPAVRAALKQYQVD
                     FANQPGGAVLDGRDIGTVIAPHAPVKLFVTAEPEVRARRRWLQLSAHDPELAYETVLD
                     DIRVRDARDSGRADAPLEKAADALLLDTTDLDIEASIAAARSLVKSRCDAG"
     gene            complement(1742..2161)
                     /locus_tag="VF402_06655"
     CDS             complement(1742..2161)
                     /locus_tag="VF402_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7800011.1"
                     /translation="MSLILLDTHVLIWMIDNSPRIGPRTRSLIEQASMAGDLCISAMT
                     VWEIAMLDSKGRLNLDRDIQVWIDTVLDEGGLSLTPLAPKIAFASTRLPDGFHSDPAD
                     RVIVATARHLHAQLITEDRLILSYADLGHVNAQSCSS"
     gene            complement(2158..>2366)
                     /locus_tag="VF402_06660"
     CDS             complement(2158..>2366)
                     /locus_tag="VF402_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7800012.1"
                     /translation="GEFKARCLKLLDEVAQTRQPLIITKHGKPVAQLTPLPPKKSPFG
                     LMAGSVLYEGDIISPIDVEWNANK"
BASE COUNT          410 a          740 c          763 g          453 t
ORIGIN      
        1 gaacttgccg ccgaccggat acttgacgtc gacgttttcc cacgggtcgg attgcagttg
       61 cttcatgccc agcgagatgc gttgcgtgtc cgggttgatc ttgatgatct ggaccttgac
      121 cgtatcgccg acgttcagca cctgcgacgg atgcgagacg cgcttccagc tcatgtcggt
      181 gacgtgcagc aggccgtcga tgccgcccag gtccacgaac gcgccgtaat cggtgatgtt
      241 cttgaccacg ccttcgcgga tctcgccctc ggccaattgg ccgaccagtt cggtgcgctg
      301 ctcggcgcgg gcttcttcga ggatggcgcg gcgcgacacg acgatattgc cgcgcggacg
      361 gtccatcttg aggatggcga agggctgttc cttgcccatc agcggggtga cgtcgcgcac
      421 cggacggata tcgacctgcg aacccggcag gaagccggaa gcgccgtcca tatcgacggt
      481 gaagccgccc ttgacgcggc cgacgatcga acccatgacc ggctcgccgc ggttgaagat
      541 ggcttcgaga cgggtccagg cttcttcgcg gcgggccttg tcgcggctga tgaccgcttc
      601 gcccatcgcg ttctcgacgc gctccagata gacctcgaca tggtcgccgg tcttcaggtt
      661 ggcgccttcg gagccgaact ccttcagcgg gatgcggcct tcggtcttca gaccgacgtc
      721 gaccgtcaca tagtcctttt cgacgccgac gacgacgccg tgaatgacct ggccttcgag
      781 catgtcgcgg ccatgcatcg attcatcgag cagggcggcg aaatcgtcgc gggagggatt
      841 aaattcgctc ataaaacaag catttctttg ggttggcgcg tgtccgctcc tgccgaatgc
      901 cgctctttcc gcaagaggcc gggcgggcga tctgcggcgg gcgtggcgcg gggtgcgact
      961 ttggattggg ttttaagctg tctgggaggc agccgcttcg acagcgacgg gccccaaggg
     1021 gtggaccgga cgtaaacgtc ccgatggcgg accctttgtg ctgcctctta aaccaccgct
     1081 gttgggattt gacgcttatc cggcgtcaca acggcttttc acaagggatc gcgcggccgc
     1141 gatggaggct tctatatcca agtcggtcgt atcaagcaag agcgcgtccg ccgctttttc
     1201 caaaggcgcg tcggcgcggc cggaatcgcg cgcgtcgcgg acgcggatat cgtccagcac
     1261 cgtctcatag gccagttcag gatcgtgcgc cgacaattgc agccagcggc ggcgcgcgcg
     1321 cacttccggt tcggcggtga cgaacagctt gaccggggca tgcggcgcga tcaccgtgcc
     1381 gatgtcgcgt ccgtccagca cggcgccgcc cggctgattg gcgaaatcga cctgatactg
     1441 cttcagggcc gcgcgcaccg ccggtatcgc cgcgacccgc gaggccatct cccccgccat
     1501 gcgcccgcgc aaggccgcgt cggccagcag atcggcgtcg atcgcttgcg ccagcgcggc
     1561 tatggcggcg tggtcgtcag gatcggcctt acgcgccaga tgcccgaccg cgcggtacaa
     1621 cagcccggtg tcgagacacg gcaggccgta atcggcggcg agaatagcgg ccaatgtgcc
     1681 cttgcccgaa gcggcgggac cgtcgacggc gatgatgaat ggcgttttca cgaactggaa
     1741 ctcacgaact gcaactctgg gcgttgacat gacccaaatc cgcgtaggac aggatcaggc
     1801 ggtcttcggt tatcaattgc gcatgaagat gccgcgccgt tgcgacaatg acccggtccg
     1861 cgggatcgct atgaaagccg tcgggcaagc gtgtgctggc gaaggcgatt ttcggcgcca
     1921 gcggcgtcag ggacagaccg ccctcatcca ggaccgtatc gatccagacc tgaatatccc
     1981 ggtccagatt gagacggcct ttcgaatcca gcatggcgat ttcccatacg gtcatggccg
     2041 aaatgcatag gtcgcccgcc atcgacgcct gctcgatcag cgagcgcgtt cgcgggccga
     2101 tacgcggtga attgtcgatc atccagatca ggacgtgcgt gtccagcagg ataagactca
     2161 tttattggcg ttccattcga cgtcaatcgg cgagataatg tcgccctcat aaagcacgct
     2221 gcccgccatc aggccgaacg gacttttctt cggcggcaag ggcgtcagtt gcgcgactgg
     2281 cttgccgtgc ttggtgatga tcagcggctg gcgggtctgc gccacctcgt cgagcagctt
     2341 caggcagcgc gccttgaact cgccgg
//
LOCUS       DASVLL010000402         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_25917, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000402 DASVLL010000000
VERSION     DASVLL010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25917"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VF402_06665"
     CDS             <1..350
                     /locus_tag="VF402_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800013.1"
                     /translation="ILTTVLIGVFSFALAPLLKGGEGAILIFALVSMALMGMTYGLLG
                     TTLAAMFPTRVRYTGASLTFNFSGIVGASLAPYIAVWMQKHYGLGAVGLYLLAAAAIT
                     LVCVLLSDKREAI"
     gene            434..>1688
                     /gene="ahcY"
                     /locus_tag="VF402_06670"
     CDS             434..>1688
                     /gene="ahcY"
                     /locus_tag="VF402_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7800014.1"
                     /translation="MADHIVRDLSLAPWGRKEIEIAEGEMPGLMATRAEFGKDKPLKG
                     ARIAGSLHMTIQTAVLIETLQALGAEVRWASCNIFSTQDHAAAAIAATGTPVFAVKGE
                     TLEEYWDYAHKIFEWPKTSEEPDGGYPNLILDDGGDATLLCVLGPKAEKDPSILDKPT
                     NEEEEALYKVMKRYLKEKPGFYSAIRDAIQGVSEETTTGVHRLYAMAQKGDLPFPAIN
                     VNDSVTKSKFDNLYGCRESLVDAIRRATDVMLAGKVAVVLGYGDVGKGSAASLRNGGA
                     RVIVTEIDPICALQAAMEGYEVRTVEEVAGQGDIFVTCTGNKDVLRLEHMREMKHNAI
                     VCNIGHFDSEIQISALRNYRWDEIKPQVHHVEFPDGKKIIVLSEGRLVNLGNATGHPS
                     FVMSASFTNQTLAQIELWTRGSHYDK"
BASE COUNT          334 a          537 c          521 g          296 t
ORIGIN      
        1 tgatcctgac caccgtgctg atcggcgtct tttcgttcgc gctggcgccg ctgctcaaag
       61 gcggcgaagg cgcgatcctg atcttcgccc tggtcagcat ggcgctgatg ggcatgacct
      121 acggcctgct cggcacgacg ctggccgcca tgttcccgac gcgcgtgcgc tataccggcg
      181 cctcgctcac cttcaatttt tccggcatcg tcggcgcgtc gctggcgccc tatatcgcgg
      241 tgtggatgca aaagcactac ggcctcggcg cggtcggcct ctacctgctg gccgcggcgg
      301 cgatcaccct cgtctgcgtg ctgctatcgg acaagcgcga agccatataa agatatgttt
      361 atgtctttat tgcgaagtga gtcctgagcc gctataagcg accgatcgca ttcgcaacag
      421 gatagacaac atcatggctg accatatcgt ccgtgacctt tctctggctc cgtggggccg
      481 caaggaaatc gagatcgccg agggcgaaat gccgggcctg atggccacgc gcgccgagtt
      541 cggcaaggac aagccgctga aaggcgcgcg catcgccggt tcgctgcaca tgaccatcca
      601 gacggcggtg ctgatcgaga cgctgcaggc gctgggcgcc gaggtgcgct gggcgtcgtg
      661 caacatcttc tcgactcagg accacgccgc cgccgccatc gccgccacgg gcacgccggt
      721 cttcgcggtc aagggcgaga cgctggagga atactgggat tacgcgcaca agattttcga
      781 gtggccaaag acttctgaag aacctgacgg ggggtatccg aacctgatcc tcgatgacgg
      841 cggcgacgcg accttgctgt gcgtgctggg tccgaaggcg gagaaagacc cgtccatcct
      901 cgacaagccg acgaacgagg aagaagaagc gctctataag gtgatgaagc gctacctgaa
      961 ggaaaaaccc ggcttctatt cggcgatccg cgacgccatc cagggcgtgt cggaagaaac
     1021 caccaccggc gtgcatcgcc tgtacgccat ggcgcagaag ggcgacctgc ccttcccggc
     1081 gatcaacgtc aacgattccg tcaccaagtc gaagttcgac aacctctacg gctgccgtga
     1141 atcgctggtc gacgccatcc gccgcgccac cgacgtgatg ctggccggca aggtcgcggt
     1201 cgtgctcggc tacggcgatg tcggcaaggg ttcggcggcg tctttacgca acggcggcgc
     1261 gcgcgtgatc gtcaccgaga tcgacccgat ctgcgcgttg caggcggcga tggaaggcta
     1321 tgaggtgcgc accgtcgagg aggtcgcggg acagggcgac atcttcgtca cctgcaccgg
     1381 caacaaggac gtgctgcgcc tcgaacacat gcgcgaaatg aagcacaacg ccatcgtctg
     1441 caatatcggc cacttcgatt ccgaaatcca gatcagcgcc ttgcgcaact atcgctggga
     1501 cgagatcaag ccgcaggtcc accacgtcga attcccggac ggcaagaaga tcatcgtgct
     1561 cagcgaaggc cgcctcgtga acctcggcaa cgccacgggc catccgtcct tcgtcatgtc
     1621 ggcctcgttc accaaccaga ccctggcgca gatcgagctg tggacgcgcg gttcgcacta
     1681 cgacaaga
//
LOCUS       DASVLL010000403         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_240290,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000403 DASVLL010000000
VERSION     DASVLL010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240290"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF402_06675"
     CDS             <1..258
                     /locus_tag="VF402_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7800015.1"
                     /translation="DAACDAACEQAYAALQGAGRDVLYNDSDERPGAKFASMDLIGVP
                     WQLIVGPKGLGEGVVELKHRASGDRQSLSLEAALAKLTHKA"
     gene            261..1547
                     /locus_tag="VF402_06680"
     CDS             261..1547
                     /locus_tag="VF402_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX7800016.1"
                     /translation="MNAILSMFAFRGWEADLAMRYLRTRRKDGGIAVIAIISFVGITL
                     AVTALISVLSIMNGFRDEMMSRVLAFQGHAYVQGTLIDDFAHRDDMLKRIKAVPGVIE
                     ATPQIDSPGLVQNAEGQTSLAYMRGVVPDDLRHTPIVADTLEKGGSMDRFGVGDYGGD
                     VVLIGDGLASDLGLIPGDQLTLTSPGGSTAFGAAPRRKVYTVGGIFKSGVSEIDKSFV
                     YMPIQQAQLFFDREDEWDQVEIKVEKPYDIEKYLPALHRATGRGAIVTTWKELNAPLW
                     GALKVERNAMRFILFFIVIIAMLNIISGIVMLVKNKARDVAILRTMGADRGAISRVFF
                     LTGTSIGAAGTLCGLMIGVLFCTFIREIQDALEWIFHVQLFNKDIYFLDHVPAKMEPS
                     EVIFVVVASLLAASVSTFFPALWASKLEPVEALRYE"
     gene            1540..2220
                     /locus_tag="VF402_06685"
     CDS             1540..2220
                     /locus_tag="VF402_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7800017.1"
                     /translation="MSKALVLRGLTRDYDIGDGRRLSVLKGVDLDVGAGELVGMIGPS
                     GSGKSSLLHAIGLLEAAPNAQLSIDGEDFAHASEAERTRARLRKIGFVYQFHHLLPEF
                     TALGNVCVPQHILGRRKDDAEARARELLTALGLADRLDHLPGQLSGGEQQRVAIARAL
                     ANNPRLLLADEPTGNLDPATSKQVFEALKAIVRAQGVAAVIATHNLDLAAHMDRVVRI
                     ENGQLVSA"
     gene            2503..>3942
                     /gene="dnaE"
                     /locus_tag="VF402_06690"
     CDS             2503..>3942
                     /gene="dnaE"
                     /locus_tag="VF402_06690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7800018.1"
                     /translation="MTENFVHLRVRSAYSLLEGAIKAYDLGKLAQKNAMPAVGIADRC
                     NLFGALEFSQTCKETGVQPIVGCALPVRGIGGEGTERWSRIPTIVLFAQNEQGYLNLT
                     KLSSIAYVDSEDDQFVPWDKVVEHNEGLILLSGGPDGPVNPLFEQNRGDEARAALQTM
                     LDTFGDRFYVELQRHDDSPNGGRKGGAAESGLVEFAYAHDAPLVATNDVQFATRDFYA
                     AHEALLCIADGAYLGQDERRKVTPEHWFKPAADMRAVFDDLPEACDNTLEIARRCAFL
                     VSKRDPILPRFDTGAGRNEAEELAFQAREGLKARFKQVKLAFPEEDYWARLEREIGVI
                     QKMGFPGYFLIVSDFIKWGKAHGIPVGPGRGSGAGSLVAWALTITDLDPLRFGLLFER
                     FLNPERVSMPDFDVDFCQERREEVISYVQGKYGRDRVAQIITFGTLQARAVLRDTGRV
                     MQLPLGQVDRLSKMVPNNPANPTTLAQAIE"
BASE COUNT          699 a         1203 c         1322 g          718 t
ORIGIN      
        1 gacgccgcct gcgacgcggc ctgcgagcag gcctatgcgg cgcttcaggg cgcagggcgc
       61 gacgtgctgt ataacgacag cgacgagcgg cccggcgcca agttcgccag catggacctg
      121 atcggcgttc cctggcagct catcgtcggc ccgaaggggc tgggcgaggg cgtggtggag
      181 ttgaaacatc gcgccagcgg cgatcgccag agcctgagcc tcgaagcggc cctggccaaa
      241 ctcacgcaca aggcgtaagc atgaacgcga tcctgtcgat gttcgccttt cgcggctggg
      301 aggccgatct ggccatgcgc tacctgcgca ccaggcgcaa ggacggcggc atcgcggtga
      361 tcgcgatcat ttccttcgtc ggcattacgc tggcggtgac ggcgctgatc agcgtcctgt
      421 cgatcatgaa cggctttcgc gatgagatga tgtcgcgcgt cctggccttt caggggcacg
      481 cctatgtgca gggaacgctg atcgacgact tcgctcaccg cgacgatatg ttgaagcgaa
      541 tcaaggctgt gccgggcgtt attgaagcga cgccgcagat cgactcgccg gggctggtgc
      601 agaatgcgga gggccagacc agtctggcct atatgcgcgg cgtcgtgccg gacgacctgc
      661 gccatacgcc gatcgtcgcc gatacgctgg aaaaaggcgg ttcgatggac cggttcggcg
      721 tcggcgatta tggcggcgac gtggtgctga tcggcgacgg actggcttcc gatctggggc
      781 ttataccggg cgatcaactg accctgacct cgccgggcgg ctcgaccgcc ttcggcgccg
      841 cgccgcgccg caaggtctat acggtcggcg gcatattcaa gagcggcgtc agcgagatcg
      901 acaagagctt cgtctatatg ccgatccagc aggcgcaact gttcttcgac cgcgaggacg
      961 agtgggacca ggtcgagatc aaggtcgaaa agccgtatga catcgaaaaa tacctgccgg
     1021 ccctgcatcg ggcgacgggc aggggcgcga tcgtcacgac ctggaaagaa ctgaacgcgc
     1081 ctttgtgggg cgcgctcaag gtcgagcgca acgccatgcg cttcatcctg ttcttcatcg
     1141 tcatcatcgc catgctcaac atcatctccg gcatcgtcat gctggtgaag aacaaggcgc
     1201 gcgacgtggc gatcctcagg acgatgggcg cggaccgggg cgcgatttcg cgggtctttt
     1261 tcctgaccgg gaccagcatc ggcgcggcgg gcaccttgtg cggcctcatg atcggcgtgc
     1321 tgttctgcac cttcatccgt gagattcagg acgccctgga gtggattttc cacgttcagc
     1381 tgttcaacaa ggacatctat ttcctcgacc atgtgccggc caagatggag ccgtccgagg
     1441 tgatcttcgt cgtcgtcgcc tcgctgctgg cggctagcgt ctccaccttc ttcccggcgc
     1501 tgtgggcgtc gaagctcgaa ccggtggagg cgctccgcta tgagtaaggc gctggttctg
     1561 cgcggcctga cgcgcgatta cgacattggc gacgggcgca ggctgagcgt gctgaaaggc
     1621 gtcgatctcg acgtcggcgc gggcgaactg gtcggcatga tcgggccgtc gggatcgggc
     1681 aagtcgtcgc tcctgcacgc catcggcctg ctggaggcgg cgccgaacgc gcaactgagc
     1741 atcgacggcg aggatttcgc ccacgcctcg gaagccgagc gcacgcgcgc ccggttgcgc
     1801 aagatcggct tcgtctatca gttccaccac ctgctgccgg aattcaccgc gctcggcaat
     1861 gtctgcgtgc cgcagcatat tctggggcgg cgcaaggacg acgccgaggc gcgggcgagg
     1921 gagctgctga cggccctggg attggcggac cggctcgacc acctgccggg gcaattgtcc
     1981 ggcggcgagc agcagcgcgt ggcgatcgcg cgggcgctgg ccaacaatcc ccggcttctg
     2041 ctggccgacg agccgaccgg caatctcgac cccgcgacct cgaagcaggt tttcgaggcc
     2101 ctgaaagcga tcgtgcgcgc tcagggcgtg gcggcggtga tcgccacgca caatctcgac
     2161 ctggccgcgc atatggaccg cgtcgtgcgg atcgagaacg gtcagctcgt ttccgcctga
     2221 aaaatcccta tccgaatcaa tgcgtttcta attttgttca cattgggggc aaaattgcgc
     2281 ttgcgtgtgg aacaaagcgg gaatatagaa caaagcaaga attgagaaca taatctgaac
     2341 acggccgccg aagcggcctc gcaagaggat gtggaaaaat gctgaacacg ctgcataacc
     2401 tgttgtcatt cgccgccgtt acgggctttg ttgttctgat gtgccatgtg gcgtctctcg
     2461 ccgcctgagg ggcgaagggg gcgctgatgg ggagcgcgcg ccatgactga gaatttcgtc
     2521 catcttcgcg tccggtcggc ctattcgctg ctggaaggcg cgatcaaggc ttacgatctg
     2581 ggcaagctgg cgcaaaagaa cgccatgccc gccgtcggca tcgccgaccg ctgcaacctg
     2641 ttcggcgcgc tggagttttc ccagacctgc aaggagacgg gcgtgcagcc gatcgtcggc
     2701 tgcgccctgc cggtgcgcgg catcggcggc gaagggaccg agcgctggtc gcgcatcccc
     2761 acgatcgtcc tgttcgcgca gaacgagcag ggctatctga acctgaccaa gctgtcgtcc
     2821 atcgcctatg tcgattccga ggacgaccag ttcgtgcctt gggacaaggt ggtcgagcac
     2881 aatgaaggcc tgatcctttt gtccggcggg ccggacgggc cggtcaatcc tctgtttgag
     2941 caaaatcgcg gcgacgaggc gcgcgccgcc ctgcaaacca tgctcgatac gttcggggat
     3001 cgcttctacg tcgagttgca gcgtcacgac gacagcccca atggcgggcg caagggcggc
     3061 gccgccgaat cgggtctggt cgaattcgcc tatgcgcacg acgcgccgct ggtggcgacc
     3121 aacgacgtgc agtttgcaac ccgcgatttt tacgcggcgc atgaggccct gctgtgtatc
     3181 gccgacggcg cctatctggg gcaggacgag cgccggaagg tcacgcctga acattggttc
     3241 aaaccggcgg cggatatgcg ggccgtgttc gacgacctgc ccgaagcctg cgacaacacg
     3301 ctggagatcg cgcggcgctg cgcctttctg gtcagcaagc gcgatccgat cctgccgcgc
     3361 ttcgacaccg gcgcgggccg caacgaggcc gaggaactgg cgtttcaggc gcgcgagggt
     3421 ctgaaagcgc gcttcaaaca ggtcaagctg gccttcccgg aagaggatta ctgggcgcgg
     3481 ctggaacgcg aaatcggcgt gatccagaag atgggttttc ccggctactt cctgatcgtt
     3541 tcggacttca tcaaatgggg caaggcgcac ggcattccgg tcgggccggg gcgggggtcg
     3601 ggcgccggtt cgctggtggc gtgggcgctg accatcaccg acctcgaccc gttgcgtttc
     3661 ggcctgctgt tcgaacgctt cctgaacccg gaacgggtgt ccatgcccga cttcgacgtc
     3721 gatttctgtc aggagcggcg tgaagaggtc atctcctacg ttcagggcaa atacggccgt
     3781 gaccgcgtgg cgcagatcat caccttcggc accttgcagg cgcgcgccgt gctgcgcgac
     3841 accggacggg tgatgcagtt gccgctgggg caggtggatc gtctgtccaa gatggtgccg
     3901 aacaatccgg ccaatccgac gacgctggcg caggccatcg ag
//
LOCUS       DASVLL010000404         5971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_295410,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000404 DASVLL010000000
VERSION     DASVLL010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295410"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VF402_06695"
     CDS             complement(<1..178)
                     /locus_tag="VF402_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX7800019.1"
                     /translation="MTKAASGDSKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREEHKIAFVKS"
     gene            437..1063
                     /locus_tag="VF402_06700"
     CDS             437..1063
                     /locus_tag="VF402_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX7800020.1"
                     /translation="MARISPSVRRARVVAFDFDGTLTCKDSFAAFLTATCGPARVGAA
                     FVLQPRLGLDYLATRDRGALKARLLRRLLGPVAQGELETLMRAFVARTGMGLFRPDAL
                     AAWRAYADTGVQRVIVTASPDLLVGGFGRLIGADRVIGTRLGFSAEGRLLPALDGENC
                     RGAEKVRRLREAFGMDFILQAAYGDTSGDKEMLAFAEEGHYRVFTQKP"
     gene            complement(1064..2488)
                     /locus_tag="VF402_06705"
     CDS             complement(1064..2488)
                     /locus_tag="VF402_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7800021.1"
                     /translation="MHDTSVLTQKTKPTGKARAVAAALLIAAGLGLNGCAVVSKLTHN
                     ANSDQGAVISQGQRISIVAFQQKLEPSKSLKGVGYYIPPAQPVTAWPVAGGDQSDPVQ
                     NAAAGKDFQIAWTRSIGVKSASDTEVLAQPVSDGKLIFTLDGQARVSAFDVDSGNQVW
                     SINLDPKIKRDKEGFGGGLAVSDGVLYVTSGYRFLIALDAATGAEKWRKALDTQLHSP
                     PTVDATQVYAADVDNQIFAFNKATGEMVWTYQAISEPARIMKSSSPVVSGDMVYAPFS
                     SGELVALTSNNGSQVWDQVLAQSTRTNALSEIRDIAGRPVLYNGVIFAASHSGAFQAM
                     DARTGAVKWTLDADSVNTPWVSGDVVFLMSLQGELMSVSRDSGQIYWLTDLDEGYLKK
                     KKGFFGMGREKTVGKVPVWTGPVVASDRLVLVNSLGQMLAFDAKSGKREDSLKLPGPA
                     YIAPIAVGDKLFVVTDDAKLVAIR"
     gene            complement(2534..3277)
                     /locus_tag="VF402_06710"
     CDS             complement(2534..3277)
                     /locus_tag="VF402_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7800022.1"
                     /translation="MSDIFDETEENLRADRVVAIVKVALPWVSAILVLALVIALGVWG
                     WQSWRATQSAHAAETYQAGLDASGKGDKATAKTKFEAAAKAGNPAYKALALMELGAMA
                     LDDNNTDEALKDFDAAAKATSSPLLSDTAALKAAYLAMDKSSLADEQKRLAPLMADKR
                     PLAAMAKEAMALAKLQSGDVNGARADLKVLGVTLGAPDGVKQRATEIVAAIDSGAAPT
                     ALAILKQPEPAAAIPNAALQSAVANAAAH"
     gene            complement(3439..4263)
                     /gene="panB"
                     /locus_tag="VF402_06715"
     CDS             complement(3439..4263)
                     /gene="panB"
                     /locus_tag="VF402_06715"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7800023.1"
                     /translation="MSRQTPPSRAKRVTAPDIRARKGQTPIVCLTCYTAPMARIMDPH
                     CDILLVGDSLGMVIHGLPNTVGVTMEMMSLHGQAVMRGAETALVVVDMPFGAYEAGPQ
                     QAFDNAVRLMKETGAQAVKLESGSAVAGTVRYLVERGIPVMGHIGLRPQAVNADGGFK
                     AKGRTAAEREKVLKEARDTDEAGAFAIVVEGVAADLAEEITRAVQAPTIGIGASAACD
                     GQVLVVDDMLGLFDWTPRFVRHYAELRTQIGEAVAAYAEDVRTRDFPAPEETYFSK"
     gene            complement(4298..4756)
                     /locus_tag="VF402_06720"
     CDS             complement(4298..4756)
                     /locus_tag="VF402_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800024.1"
                     /translation="MTFDPDPDHGFSDRDFEARLKSLFSEQPRYADAEGFCARLMFAL
                     RLKLWLRQGFIVLAGLIGGVYALAQFIRAPELRGRHVLTLKQAAADTDHTLRAGAAMM
                     SYARQNAGGWLDSWAHYLGVMQSPIFFWVSFTLCLACLALYYAYSQEERI"
     gene            complement(4774..5361)
                     /locus_tag="VF402_06725"
     CDS             complement(4774..5361)
                     /locus_tag="VF402_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7800025.1"
                     /translation="MAERGATSEARSDLTRFSDVDLIRLCAGGDRPVFAELARRHSGT
                     LRTALRRMGAAPHDADDMAQEALMIAYERLEEYRFDGPFAAWLKRIAARRYLKRVRGS
                     ARYMLTDDPAAYEAEAPQDDGFRAHNLEAALNRLKPVERLCVSLSFAGDLSHQDIADE
                     LKLPLGTVKSHLKRALERLRAILAPSETPARDSVS"
     gene            complement(5371..5760)
                     /locus_tag="VF402_06730"
     CDS             complement(5371..5760)
                     /locus_tag="VF402_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX7800026.1"
                     /translation="MDWDSVTGILAIIMIFGMPVFIIAIIFYFLYRSRQETQKTLRMA
                     IEKSDNLPPEFLDNLKAMQKKPKTPANDIRAGVILIAIALGLVALDAATHGYVIGSLS
                     GVAAIPGFIGVALLILGAIGSRKPSQG"
BASE COUNT          985 a         1980 c         1886 g         1120 t
ORIGIN      
        1 tcgatttcac gaaggcgatc ttgtgttcct cgcgaatgat gtccatgcac agttcgacgc
       61 attcgtcgca gatgaacacg gtcggcccgg cgatgagctt gcgcacttcg tgctggctct
      121 ttccgcagaa cgagcagtaa agggtgcttt ttgaatcgcc agaagcggct ttggtcatag
      181 gtttacaatc cttagcggcg cctgaatacg tcgcgacgcg tctcttcacg acacgagtct
      241 tacgcccaca agccttaaga taaggctgat tttggcacag acgcacgtca catttggttc
      301 acagacctgc gattatgcac agtttagacc acgtctcaac agcacatgca gcgtcaggct
      361 tagcttaacg ctctttatat gggataaccg ctacagtttg taagtgcgcg agtcgcgcat
      421 ccgtaccctg agcatcatgg cccgtatatc tccttccgtc cggcgcgcgc gcgtcgtcgc
      481 tttcgatttc gacggcacgc tgacctgcaa ggacagcttc gccgccttcc tgaccgcgac
      541 ctgcggcccg gcccgcgtcg gcgccgcttt cgttctgcaa ccgaggctcg ggctcgatta
      601 tctggcgacg cgcgaccgcg gcgcgctcaa ggcgcggctg ctgcgccgcc tgctggggcc
      661 ggtcgcgcag ggcgaactgg agaccctgat gcgcgccttc gtcgcgcgca cgggcatggg
      721 tctgttccgt cccgacgcgc tggcggcgtg gcgggcctat gccgatacgg gcgtgcagcg
      781 cgtgatcgtg accgcttcgc ccgatctgct ggtcggcggt ttcggcaggc tgatcggcgc
      841 ggaccgggtg atcggcacgc gcctcggctt ctccgccgaa ggccgcctgc tgcccgcgct
      901 cgacggcgag aactgccgcg gcgcggaaaa ggtgcgccgc ctgcgcgaag ccttcggcat
      961 ggatttcatt ctgcaagccg cctatggcga cacctccggc gacaaggaga tgctggcctt
     1021 cgccgaggaa ggccactacc gcgtcttcac gcaaaagccg tgatcagcgg atagccacca
     1081 gcttggcgtc gtcggtcacg acgaacagct tgtcgcccac cgcgatcggc gcgatatagg
     1141 ccggtccggg cagtttcagg ctgtcctcgc gcttgccgct cttggcgtcg aacgcgagca
     1201 tctggcccag cgaattgacc agcaccagac ggtccgacgc caccacgggc ccggtccaga
     1261 ccggaacctt gccgaccgtc ttttcgcgcc ccatgccgaa gaagcccttt ttcttcttca
     1321 ggtagccttc gtcgaggtcc gtcagccagt agatctggcc ggaatcgcgc gacacgctca
     1381 tcaactcgcc ttgcaggctc atcaggaaca cgacgtcgcc cgacacccac ggcgtattga
     1441 cgctgtcggc gtccagcgtc cacttcacgg cgccggtgcg cgcgtccatc gcctgaaagg
     1501 cgccggaatg gctggcggcg aagatcacgc cgttatagag gacgggacgc ccggcgatgt
     1561 cgcgaatctc ggacagggcg ttggtgcggg tcgattgcgc cagcacctga tcccagacct
     1621 ggctgccgtt attgctcgtc agggcgacca gctcgcccga ggaaaacggc gcatagacca
     1681 tgtcgcccga cacgacgggc gacgacgact tcatgatgcg cgccggttcg gagatggcct
     1741 gataggtcca gaccatttcg cccgtcgcct tgttgaaggc gaagatctgg ttgtcgacgt
     1801 cggcagcgta aacctgcgtc gcgtccaccg tcggcggcga atgcagttgc gtatccagcg
     1861 ccttgcgcca cttttccgcg cccgtggccg cgtccagcgc gatcaggaag cgatagccgg
     1921 aggtcacgta cagcacgccg tccgacacgg ccagaccgcc gccgaagcct tccttgtcgc
     1981 gcttgatctt cggatcgaga ttgatcgacc agacctgatt gccgctgtcg acgtcgaagg
     2041 ccgaaacgcg cgcctgtccg tccagcgtaa agatcagctt gccgtccgag acgggctgcg
     2101 ccagcacctc ggtgtcgctg gccgacttca cgccgatgga ccgcgtccag gcgatctgga
     2161 aatccttgcc cgccgcggcg ttctgaaccg gatcgctctg gtcgccgccc gccacgggcc
     2221 acgccgtcac gggctgcgcg ggcggaatat agtagccgac gcccttgagg ctcttggacg
     2281 gttcgagctt ctgctggaag gcgacgatcg agatgcgttg cccctgcgag atcaccgccc
     2341 cctgatcgct gttggcgttg tgcgtcagct tgctgaccac ggcgcagccg ttcaggccca
     2401 gcccggcggc gatcagcagc gccgccgcga cggcgcgcgc cttgcctgtc ggcttcgtct
     2461 tctgggtcag gactgacgtg tcgtgcatga gagcgggttc catcatgaaa ttatgtgcgg
     2521 cgctaaagcc ggattagtgc gcggcggcgt tggcgacggc gctttgcagc gccgcgttcg
     2581 ggatcgccgc ggcgggctcg ggttgcttga gaatcgccag cgcggtcggg gccgcgcccg
     2641 aatcgatggc ggcgacgatt tccgtggcgc gctgcttgac cccgtccggc gcgcccagcg
     2701 tcacgcccag caccttcaga tcggcgcgcg cgccgttgac gtcgccgctt tgcagcttgg
     2761 ccagggccat cgcctccttg gccatcgcgg ccagcgggcg cttgtcggcc atgagcggcg
     2821 ccaggcgctt ctgctcgtcg gccagcgaac ttttgtccat ggccagatag gcggccttga
     2881 gcgcggcggt gtcggacagc agcggcgagg aggtcgcctt ggccgccgcg tcgaaatcct
     2941 tgagcgcctc gtcagtgttg ttgtcgtcca gcgccatcgc gcccagctcc atcagggcca
     3001 gcgccttata ggccggattg cccgccttgg ccgccgcctc gaatttcgtc ttcgccgtcg
     3061 ccttgtcgcc cttgccgctg gcgtccagtc cggcctgata ggtttcggcg gcgtgcgcgc
     3121 tctgcgtggc gcgccagctt tgccagcccc agacgcccag agcgatcacc agagccagaa
     3181 cgaggatcgc gctgacccag ggcaaggcga ctttgacgat ggcgaccaca cggtcggcgc
     3241 ggaggttctc ttcggtttcg tcaaaaatat cgctcacggc gaatccttct gttcataggc
     3301 ttgggggccg gcggcttggg gtccggcgcg agcgcccgcc ggccgacagg ccgcgaaact
     3361 agcctctgac cggcgattaa gcaacatgcc gccgcgcggc ggcgcgtctt ttgcgacgat
     3421 taccccagag cccggcattt attttgagaa atatgtctct tctggggcgg gaaaatcgcg
     3481 cgtcctgaca tcttcggcgt aagcggccac cgcctcgccg atctgcgtac gcaattcggc
     3541 gtagtggcgc acgaagcgcg gcgtccagtc gaacaggccc agcatatcgt cgaccaccag
     3601 cacctgaccg tcgcaggccg ccgaggcgcc gatgccgatg gtcggcgcct gcaccgccct
     3661 ggtgatctcc tcggccagat cggcggcgac gccctcgacc acgatggcga aggcgcccgc
     3721 ctcgtcggtg tcgcgcgcct ccttcagcac cttttcgcgc tcggctgcgg tgcggccctt
     3781 ggccttgaag ccgccgtcgg cgttgaccgc ctgcgggcgc aggccgatat ggcccatgac
     3841 cggaatgccg cgctcgacca gatagcgcac cgtcccagcg accgccgagc cgctttccag
     3901 cttgaccgcc tgggcgccgg tctccttcat caggcgcacc gcgttgtcga acgcctgttg
     3961 cggcccggcc tcgtaggcgc cgaacggcat gtcgaccacg accagcgccg tctcggcgcc
     4021 gcgcatcacc gcctgaccgt gcaggctcat catctccatc gtcacgccga ccgtgttggg
     4081 caggccgtgg atcaccatgc cgaggctgtc gccgaccagc aggatgtcgc aatgcgggtc
     4141 catgatccgc gccatcggcg cggtgtagca ggtcaggcag acgatcggcg tctggccctt
     4201 gcgggcgcgg atatcgggcg cggtgacgcg cttggcgcgg ctgggcggcg tctggcggct
     4261 cataggggcc tcctgttgtc tatgcgaaaa acgaacctca gattcgttct tcctgagaat
     4321 aggcgtaata cagcgccaga cacgccagac atagggtaaa actgacccag aagaagatcg
     4381 ggctttgcat gacgcccaga taatgcgccc aggaatcgag ccagccgccc gcgttctggc
     4441 gcgcatagct catcatggcg gcgcccgcgc gcaaggtgtg gtcggtgtcc gccgccgcct
     4501 gtttcagcgt caggacgtgg cggccgcgca gttccggcgc gcggatgaac tgggccagcg
     4561 catagacgcc gccgatcagg cccgccagca cgataaagcc ctgacgcagc cacagtttca
     4621 ggcgcagcgc gaacatgagg cgggcgcaga atccttcggc gtccgcgtaa cgcggctgct
     4681 cgctgaacag gcttttcagg cgcgcctcga aatcgcggtc agaaaagccg tgatcggggt
     4741 cgggatcgaa tgtcatggtc cggtttcctg atgtcaggac acgctgtcgc gcgccggggt
     4801 ttccgaaggc gccagaatcg ccctgaggcg ttccagcgcg cgtttcaaat gactcttcac
     4861 cgtgccgagc ggcagtttca gctcgtcggc gatgtcctga tggctcagat cgcccgcgaa
     4921 gctgaggctg acgcacaagc gttccaccgg tttgaggcga tttaaggccg cttcgagatt
     4981 gtgcgcgcga aaaccgtcgt cctgtggcgc ctccgcctca taggcggcgg ggtcgtcggt
     5041 cagcatgtag cgcgcactcc cgcgcacgcg cttcagatag cgccgcgccg cgatgcgttt
     5101 cagccacgcc gcgaacgggc cgtcgaagcg gtattcctcc agccgttcat aggcgatcat
     5161 cagcgcctcc tgcgccatgt cgtcggcgtc gtgcggcgcc gcgcccatgc ggcgcagggc
     5221 cgtccgcaac gtccccgaat gacggcgcgc cagttcggcg aagaccggcc tgtcccctcc
     5281 ggcgcacagc cggatgagat cgacgtcgga aaaccgcgtc agatcggatc tcgcctctga
     5341 tgtcgcgccg cgctcagcca tagacgaagg tcagccctgc gacggcttgc ggctgccgat
     5401 ggcgcccagg atcagcaggg cgacgccgat aaagcccgga atggcggcca cgccggacag
     5461 gctgccgatg acgtagccgt gcgtcgccgc gtccagcgcc accagaccca gcgcgatcgc
     5521 gatcaggatc acccccgcgc ggatgtcgtt cgccggagtc ttcggctttt tctgcatcgc
     5581 cttgagattg tccagaaact ccggcggcag gttgtcgctc ttttcgatcg ccatgcgcag
     5641 ggtcttctga gtctcctgcc ggctgcgata gaggaagtag aagataatgg cgattataaa
     5701 gaccggcatg ccaaaaatca tgatgatggc cagaatgccc gtcacgctat cccaatccat
     5761 tgcgatgctc ctttcggttc ggacccgcct caggtccgtt catcatcaga gtctttcaac
     5821 ccgccttttg gatgcacgga ctcgcattaa gttttcgtaa tgcgattttt tccgcgaatt
     5881 tttaggcgcc ctcttgcaaa agatagaatt tagatatatc tgaattgcat cgaaaagacg
     5941 gaaggcgata ccgaaccctt ctttcgattc g
//
LOCUS       DASVLL010000405         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_295492,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000405 DASVLL010000000
VERSION     DASVLL010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295492"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VF402_06735"
     CDS             <1..736
                     /locus_tag="VF402_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7800027.1"
                     /translation="FLVARKDIKLDGSTPTLLYAYGGFEVSMQPSYSASVGKLWLEKG
                     GAYVLANIRGGGEFGPKWHEAGLKTHRQRIYDDFQAVAEDLIAKKITSTPRLGIMGGS
                     NGGLLMGVQLTERPDLWNAVVIQVPLLDMVNFNHMSAGASWEGEYGDPDDPVEGAFLR
                     SISPYHNVRAGVAYPEPFFETSTKDDRVGPVHARKMAALFEDMGLPFYYYENTEGGHA
                     AAANLQERARRYALEFTYLSEKLMDK"
     gene            complement(776..1957)
                     /locus_tag="VF402_06740"
     CDS             complement(776..1957)
                     /locus_tag="VF402_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800028.1"
                     /translation="MKHTQPIPAVTAFANDSDFISVREIRNLGHLGRTWSSSRVLNLA
                     KLYTAFRENADYLAKPMFENSGLNRALILKHTLRLDERHLFPGGRQTVTKVILPYDPF
                     DLRLGGRSFFFRQSRYEELMRTYLGVDDVGRNRDAKVLRCIDNLPSLDPFLLREHLAR
                     AGFVPSGVYFQISGSDLKSMASFTANEIENLVNVAFGQKRGGKGAAKLGNRILSDELH
                     KDLDPLRETLRMEEEAFQEGIICWRGFLYYKWRHRELQGGVREVLTGLGAIRPRAAHN
                     DATSLYLKKAVPRVARAIAAVINDTRITLAEYDEVYRALAVKRDPEPFSHFLRYGSSL
                     FLDLGEKIGTLDHIVSFWRFRMARTMRKGARPLHDIEYADLLMDFEAGLIQSLQKGQQ
                     P"
     gene            complement(1954..>2649)
                     /locus_tag="VF402_06745"
     CDS             complement(1954..>2649)
                     /locus_tag="VF402_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800029.1"
                     /translation="GYRPAACYLKISPHDVQRMVGFANAEIEQLVRIAFTEVMGEGAA
                     GKLAGKILSHELDAELRPLMTTLNMTEAEFSDGIFSWRGFLYFKWRQNELREEMRRVL
                     EALANYQPSGACDDALRDYFRDARPRLARRIIDAIAHAGRVLSVYDDAYNALVKGQDA
                     GPFHRFLLDGPALFFELGECIGILSHISSFWDYRVTRTLNSQHLSAVDYGDILMDFED
                     ALSLVTRYSEGRP"
BASE COUNT          473 a          848 c          879 g          449 t
ORIGIN      
        1 cttcctcgtg gcgcgcaagg acatcaaact ggacggctcg accccgaccc tgctctacgc
       61 ctatggcggc ttcgaggtgt cgatgcagcc gtcctattcg gcctcggtcg gcaagctgtg
      121 gctggaaaag ggcggcgcct atgtgctggc caatatccgc ggcggcggcg agttcgggcc
      181 gaaatggcat gaggcggggc tgaaaacgca ccgtcagcgc atctatgacg actttcaggc
      241 cgtcgccgaa gacctgatcg cgaagaagat cacctcgacg ccgcgcctcg gcatcatggg
      301 cggctcgaac ggcggcctgc tgatgggcgt gcaactgacc gaacgcccgg atctgtggaa
      361 cgcggtggtg attcaggtgc cgctgctcga catggtgaac ttcaaccaca tgtcggcggg
      421 cgcgtcatgg gaaggcgaat acggcgaccc cgacgatccg gtcgaaggcg ccttcctgcg
      481 ctcgatctcg ccctatcata acgtcagggc gggcgtcgcc tatcccgaac ccttcttcga
      541 gacctcgacc aaggacgacc gcgtcggccc cgtccacgcg cgcaagatgg ccgccctgtt
      601 cgaggatatg ggcctgccct tctactatta cgaaaacacc gaaggcggcc acgcggcggc
      661 ggcgaacttg caggaacgcg cccgccgcta cgcgctggaa ttcacctatc tgtccgaaaa
      721 gctgatggac aagtaagcgt cagggcgccc gttgcaggcc gccctcacgc gaatctcacg
      781 gctgctggcc cttttgcagc gactggatca gccccgcctc gaaatccatc aggaggtcgg
      841 cgtattcgat gtcgtgcagc gggcgggcgc ccttgcgcat ggtgcgcgcc atgcggaagc
      901 gccagaacga gacgatatgg tccagcgtgc cgatcttctc gccgaggtcg aggaacaggc
      961 tggagccgta gcgcaggaag tggctgaacg gctccgggtc gcgcttgacc gccagcgcgc
     1021 ggtaaacctc gtcatattcg gccagcgtga tgcgcgtatc gttgatgacg gcggcgatgg
     1081 cgcgcgcgac gcgcggcacg gccttcttga gatagaggct ggtggcgtcg ttgtgcgcgg
     1141 cgcgcgggcg gatggcgccc agccccgtca gcacctcgcg caccccgccc tgaagctcgc
     1201 ggtggcgcca cttgtaataa aggaagccgc gccagcagat gataccctcc tgaaacgcct
     1261 cctcctccat gcgcagcgtc tcgcgcagcg ggtccagatc cttgtgcagt tcgtccgaca
     1321 ggatgcggtt gcccagcttg gccgcccctt tcccgccgcg cttctgaccg aaggcgacgt
     1381 tgacgaggtt ctcgatctcg ttggcggtga agctggccat cgacttcagg tccgaacccg
     1441 aaatctggaa atagacgccc gacggcacga aaccggcgcg cgccagatgc tcgcgcagca
     1501 ggaacggatc gagcgagggc aggttgtcga tgcagcgcaa caccttggcg tcgcggttgc
     1561 gcccgacgtc gtcgacgccg agataggtgc gcataagctc ctcgtaccgg ctctggcgga
     1621 agaagaagga gcgcccgccc agacgcaggt cgaaagggtc gtagggcagg atgaccttgg
     1681 tcacggtctg gcgcccgccg gggaacagat ggcgctcgtc cagccgcagc gtgtgcttga
     1741 ggatcagggc gcggttcagc cccgaattct cgaacatcgg cttggccaga tagtcggcgt
     1801 tctcgcggaa cgccgtgtac agcttggcca ggttcagcac gcgcgacgac gaccaggtgc
     1861 ggccgaggtg gcccagattg cggatctcgc gcaccgagat gaagtcgctg tcgttggcga
     1921 aggcggtgac ggcgggaatg ggctgggtgt gcttcacggg cgtccctcgc tgtagcgggt
     1981 gaccagcgac agggcgtcct cgaaatccat caggatgtcg ccgtagtcga cggcgctcag
     2041 atgctggctg ttgagggtgc gcgtcacgcg gtagtcccag aacgaactga tatggctgag
     2101 gatgccgatg cattcgccca gctcgaagaa cagggccggt ccgtccagca ggaaacggtg
     2161 gaacggcccg gcgtcctgcc ccttgaccag cgcgttatag gcgtcgtcgt acacgctcag
     2221 cacgcgcccg gcatgggcga tggcgtcgat gatgcggcgc gccagacgcg ggcgcgcgtc
     2281 gcggaaatag tcgcgcaagg cgtcgtcgca ggcgcccgaa ggctggtaat tggccagcgc
     2341 ctccagcacc cggcgcatct cctcgcgcag ttcgttctgc cgccatttga agtacaaaaa
     2401 gccgcgccag gaaaagatcc cgtccgagaa ctccgcctcg gtcatgttca atgtggtcat
     2461 cagcggacgc aattccgcgt ccagctcgtg cgacaggatc ttgcccgcca gcttgcccgc
     2521 cgcgccctcg cccatcacct cggtgaaggc gatgcgcacc agttgctcga tttcggcgtt
     2581 ggcgaagccc accatgcgct gcacgtcgtg cggcgagatt ttcaggtagc acgccgccgg
     2641 cctgtaccc
//
LOCUS       DASVLL010000406         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_347973,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000406 DASVLL010000000
VERSION     DASVLL010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347973"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF402_06750"
     CDS             complement(<1..256)
                     /locus_tag="VF402_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7800030.1"
                     /translation="MSNDEIAIELPQDKLTGAALIKREAALAPDSPGVYRMLGHDGEV
                     LYVGKAKSLKKRILQYAQGRFHTQRIARMVALTHEMVLLRA"
     gene            complement(324..581)
                     /locus_tag="VF402_06755"
     CDS             complement(324..581)
                     /locus_tag="VF402_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7800031.1"
                     /translation="MPKTVFTGHHQDLVQTLIAARKASGLTQTELAERLGKDQTFISL
                     VERSQRRIDVLEFYAYAKALGVNPLELYAELISRLPEKLPI"
     gene            741..1157
                     /locus_tag="VF402_06760"
     CDS             741..1157
                     /locus_tag="VF402_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800032.1"
                     /translation="MKVLIASTAVMASLAFFATSANAQYYQQVYAYPAYVASYGYYLQ
                     TPATVTMVAPVSIAPAAPMPAIPVAQVSVAQTVVAAAPAAPVVQTAAVPVTQTALVAA
                     APVAAAVQPAAVPVTQVPVTPTAVVAAAPVVLFSAF"
     gene            1219..1809
                     /locus_tag="VF402_06765"
     CDS             1219..1809
                     /locus_tag="VF402_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800033.1"
                     /translation="MKMLLASTAVMASLALFATSANAQYYQQVYAYPAYVASYGYYLQ
                     APVAVAAVAPAVQMAQVPVAAPQAPVMSVVQPAAVRLTQAPVTQPVVVAAPVAQYAPQ
                     VVSVRLRHGDDESAKRCAFADDSIGYALNNYSQLLRNQLPRDMAKYYGDQMYAQTYNL
                     RNHIRAIKYSGNAAVCEYEGDVAIRTIQSIYQTTPR"
     gene            1953..3071
                     /locus_tag="VF402_06770"
     CDS             1953..3071
                     /locus_tag="VF402_06770"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7800034.1"
                     /translation="MSQTQSSTPRGEIRALTGLRGIAALYVVLFHANGLHAFPQAVRP
                     FVHHGYMAVDLFFLLSGFVMALTYADLFRDGFDLGNFKKFLLLRLARIYPLYILVTLI
                     TAVMITTVLADTYHFDDNVLRALPFNLTMTHVWGLAYSIVPPSWSISTEWAAYLLFPL
                     TIMVAVWLPRRYALIGVGIAFAVLAFVAFGPYWMTHKHNMHGTLDVVHSYAPGTTLRC
                     LASFYIGLVAFRFRERIPPFAASLAMIAALILLCFKSTDLGLVAVFAVLIMGLSHDKG
                     PVARVLQNPVIYWLGIVSYALYLVHDLIQKIVLKSFPAWGLSGGLSRSEWVWVSVAAS
                     LGVAALAHYAFEKPSRRWARALFLREKAAPAPAAIPAE"
BASE COUNT          521 a         1041 c          904 g          657 t
ORIGIN      
        1 gcgcgcgcaa aagcaccatt tcgtgcgtca gcgccaccat gcgcgcgatg cgctgggtgt
       61 gaaagcgtcc ctgcgcgtat tgcaggatgc gcttcttcaa ggacttggcc ttgccgacgt
      121 acagcacctc gccgtcatgg cccagcatcc ggtaaacgcc cggcgaatcc ggcgccaaag
      181 cggcctcgcg cttgatcagc gccgcgccgg tgagcttgtc ctgcggcagt tcgatggcga
      241 tctcgtcgtt actcataagc gcacattagc atgacccgaa gcccatatat ggcggcaagc
      301 gcgcgaaaag ccacgcgcgc cgctcatatc ggcaattttt ccggcaaccg gctgatcagc
      361 tcggcgtaca gctccagcgg attgacgccc aaagccttgg cgtaggcata aaattccagc
      421 acgtcgatgc gcctttgcga ccgctccacc agcgaaataa aggtctgatc cttgccgagc
      481 cgctcggcca actcggtctg agtcaggccg gaagccttcc gcgccgcgat caatgtttgc
      541 acgaggtcct gatggtgacc tgtaaagacc gtcttgggca tgagcctgat tccctgaacc
      601 aagcccttta attatatggc atttttcctt acgcaaattt tccatagtga ttttttccgt
      661 attttaaccg gctggaaatg atttagggta aaataaacct ggactacaga gcgtctaaac
      721 tgactcagaa ggagtttaag atgaaagttc ttattgcctc gacagccgtc atggcttcgc
      781 ttgcgttctt cgccacaagt gcaaacgcgc aatactatca gcaggtctat gcctatccgg
      841 cgtatgttgc gtcctacggc tattatctcc agacgcctgc gaccgtgacc atggtcgcgc
      901 ccgtttcgat cgcgccggcg gcgccaatgc ccgccattcc ggtggctcag gtttccgtcg
      961 cgcagaccgt cgtcgcggcg gcgccggcgg cgcccgtcgt tcagacggcc gccgtgcccg
     1021 tcacgcagac cgctcttgtc gccgccgcgc cggtcgccgc cgccgttcaa ccggccgccg
     1081 ttcccgtgac gcaggttccc gtcacgccga ccgccgtcgt cgcggcggcg cccgtggtgc
     1141 ttttctccgc gttttgacga gcggatgcga tccgcggcgg tccccggatc gtctcggtta
     1201 actcagaagg agtttacgat gaaaatgctt ctcgcctcga cggcggtcat ggcttcgctc
     1261 gcccttttcg ccacaagcgc aaacgcgcaa tattaccagc aggtctatgc ctatccggcg
     1321 tatgttgcgt cctacggcta ttatctccag gcgcctgtgg ccgtggcggc ggtcgcgccc
     1381 gccgttcaga tggcgcaggt tcccgtcgcc gcgcctcagg ctccggttat gtccgtggtt
     1441 cagcccgctg ccgtccggct gacgcaggcg cccgtgacgc aacccgtcgt cgtcgcggcg
     1501 cccgtcgcgc aatatgcgcc tcaggtcgtc tccgtccgcc tcagacacgg cgatgacgag
     1561 agcgcgaaac gctgcgcctt cgcggacgat tccatcggct acgccctgaa caattattcg
     1621 cagcttctgc gcaatcagtt gccgcgcgac atggcgaaat actacggcga ccagatgtat
     1681 gcgcagacct acaatctgag aaaccatatc cgcgccatca aatattccgg caatgcggcc
     1741 gtctgcgaat atgagggcga cgtggcaatc aggaccatcc agtccatcta tcagaccacg
     1801 ccgcgctgaa cgataaaagc gggaagccac gacgcggctt cccgttttca caggacgctt
     1861 ctccgatccc cgaccgcgcc tgtaacattc ctccgttcac ggagccgtga ttgtgatttt
     1921 gcattcgcgt cacaatgccg taaagcgggc gcatgtctca aacccaaagc tccacgccgc
     1981 gcggcgagat cagggcgctg accggcctgc gcgggatagc ggcgctgtat gtcgtgctgt
     2041 tccatgccaa cggcctgcac gcctttccgc aggccgtgcg cccgttcgtg catcacggct
     2101 atatggcggt ggacctgttc tttctgctca gcggcttcgt catggccctg acctatgccg
     2161 acctgttccg cgacggcttc gatctcggca atttcaagaa gttcctgctg ctgcggctgg
     2221 cgcgcatcta tccgctctac attctggtca ccctgatcac ggcggtgatg atcaccaccg
     2281 tgctggccga cacctaccat tttgacgaca acgtgctgcg cgccctgccg ttcaacctga
     2341 ccatgacgca tgtctggggg ctggcctatt ccatcgtgcc gccgtcatgg tcgatcagca
     2401 ccgaatgggc ggcctatctg ctgtttccgc tgaccatcat ggtcgcggtc tggttgccgc
     2461 gccgctatgc gctgatcggg gtggggatcg ccttcgccgt gctggccttc gtggccttcg
     2521 ggccgtactg gatgacgcac aagcacaata tgcacggcac gctcgacgtc gtgcattcct
     2581 atgcgccggg cacgacgctg cgctgcctcg cctccttcta tatcggcctc gtcgcctttc
     2641 gcttccgcga gcgaatcccg ccgttcgcgg ccagtctggc catgatcgcg gcgctgatcc
     2701 tgctgtgctt caaaagcacc gatctcggcc tcgtcgcggt gttcgcggtg ctgatcatgg
     2761 gcctgagcca cgacaagggg ccggtggcgc gcgtgctgca aaacccggtg atctactggc
     2821 tcggcatcgt gtcctatgcg ctctatctgg tccacgacct gatccagaag atcgtcctca
     2881 aatcgttccc ggcctggggc ctgagcggcg gcctgtcgcg ctccgaatgg gtgtgggtgt
     2941 cggtcgccgc gtcgctgggc gtcgccgccc tggcccacta tgcgttcgag aagccgtcgc
     3001 ggcgctgggc gcgcgcgctg ttcctgcgcg aaaaggccgc gcccgcgccg gcggcgattc
     3061 cggccgaata gctcaccatt tcggatcggg cgcggccccg cccagaacct cgcggatgcg
     3121 ccg
//
LOCUS       DASVLL010000407         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_26081, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000407 DASVLL010000000
VERSION     DASVLL010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26081"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="trmFO"
                     /locus_tag="VF402_06775"
     CDS             complement(<1..1016)
                     /gene="trmFO"
                     /locus_tag="VF402_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX7800035.1"
                     /translation="MSNAPSPVHVIGGGLAGSEACWQIASAGVPVILHEMRGAIRDGA
                     PITTDAHQSDDFAELVCSNSFRSDDWEYNAVGLLHAEMRRAGSLILSCGDAHQVPAGG
                     ALAVDREGFARAVTEALSRHPLITVMREEVTRLDDKPWDNIVCATGPLTSEALSRFVL
                     DVSGEGQLSFFDAIAPIVHYDSIDMSKAWKQSRYDKEGPGGDAAAYINCPMDKDQYEA
                     FIDALLNGPKAEFKDWEHVPYFDGCLPIEVMAERGRETLRYGPMKPVGLTNPHNPAVK
                     PHAIIQLRQDNALGTLYNMVGFQTKLKHGAQTDIFRMIPGLENAQFARLGGLHRNTFI
                     QSPK"
     gene            1172..1966
                     /locus_tag="VF402_06780"
     CDS             1172..1966
                     /locus_tag="VF402_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800036.1"
                     /translation="MGFSLKKAQMEAVGFLPAAWRGAWLILLALLAVSVAAPYALKFA
                     PVAAAAGGLLALLWALKLMALGALYRLALFGRTARAEGLGFGGVQTGRSEGRLLIAQI
                     ALILFLWLIAATLATVFMLAFNLSHLGDGYIDTVSAVSAVFERRQGLDWAFVVYAALA
                     VFLLVVLSVRLSLMYAASVAERRIVTLNALSLSEGAGLKLLIGTVIVLLPLALAMGLV
                     HVLRGVAHPAAALHTVLCAVAILLVLPLSAGFFASAYRQIRDNRAK"
     gene            2018..>2759
                     /locus_tag="VF402_06785"
     CDS             2018..>2759
                     /locus_tag="VF402_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800037.1"
                     /translation="MTLSFDFAFEGFRLIRQRPKLILFWGLLSLIGNSAITAILAGMA
                     GPALASLAHMQGQAPDQQTALSLMSRIMPAMMTSLPIYVLMSAVLSAAICRAVGGDSD
                     DRFGFLGFGKRECLLIALEIVMLLLRAVAMTGFLVFGELASQILPGPAGEALLSLFSL
                     AGMGVALWLSVRLSLNTAQTFDEGRLNIWGSFELSRERFWTLLIGYATAFGLALVVMF
                     LCKQIIDGALVIASGMTSAKGSDALDLSS"
BASE COUNT          407 a          831 c          972 g          549 t
ORIGIN      
        1 attttcggcg actggatgaa ggtgttgcgg tgcaggccgc cgaggcgcgc gaactgggcg
       61 ttttccagcc ccggaatcat gcggaagata tcggtctgcg cgccgtgctt cagcttggtc
      121 tggaagccga ccatgttgta gagcgtgccc agcgcgttgt cctggcgcaa ctggatgatg
      181 gcgtgcggct tgacggcggg attgtgcggg ttggtcagcc cgaccggctt catcggccca
      241 tagcgcagcg tctcgcgacc gcgctcggcc atcacctcga tgggcaggca gccatcgaaa
      301 tagggcacat gctcccagtc cttgaactcg gccttcggtc cgttcagcag cgcgtcgatg
      361 aaggcttcgt actggtcctt gtccatcggg cagttgatat aggccgccgc gtcgccgccg
      421 gggccttcct tgtcgtagcg tgattgcttc cacgccttcg acatgtcgat ggagtcgtaa
      481 tgcacgatcg gcgcgatggc gtcgaagaag ctcaactgcc cttcgccgct gacgtcgagg
      541 acgaagcgcg acagggcctc cgaggtcagc gggccggtgg cgcagacgat gttgtcccac
      601 ggcttgtcgt cgaggcgcgt gacctcctcg cgcatgaccg tgatcagcgg atgccgcgac
      661 agcgcctcgg tgacggcccg cgcgaagcct tcgcggtcga cggccagcgc gccgcccgcc
      721 ggaacctgat gcgcgtcgcc gcacgacagg atcagcgacc cggcgcggcg catctcggcg
      781 tgcagcaggc cgaccgcgtt atattcccag tcgtcggagc ggaacgagtt ggagcagacc
      841 agttcggcga aatcgtcgct ctggtgcgcg tcggtcgtga tcggcgcgcc gtcgcggatc
      901 gcgccgcgca tttcgtgcag gatgacggga acgcccgccg aggcgatctg ccagcaggct
      961 tcggaaccgg cgagaccgcc gccgatgacg tgaacggggg aaggggcgtt tgacatggcg
     1021 cggctgtaca gcatgcgggc gcgcctgtga agatgcggcg cgcgggttga aaatctccgc
     1081 ttttcacgaa ataagcttct caggcgaagg gaattgccgc aaaacgtcga taagggtgtt
     1141 atgcggacgc agatcggcca tcgggggaca tatgggcttt tcgctgaaaa aggcgcagat
     1201 ggaggccgtg ggctttctgc ccgcggcgtg gcgcggcgcg tggctgatcc tgctggcctt
     1261 gctggcggtg agcgtcgccg cgccgtatgc gctgaaattc gcgccggtcg ccgcggcggc
     1321 gggcgggttg ctggcgctgt tgtgggcgtt gaagctgatg gcgctggggg cgctgtaccg
     1381 gttggccctg ttcggcagga cggcgcgcgc cgaaggtctg ggtttcggcg gcgtgcagac
     1441 cggacgatcc gaagggcggc tgctgatcgc gcagatcgcc ctgatcctgt tcctctggct
     1501 gatcgcggcg acgctggcga cggtgttcat gctggccttc aatctgtcgc atcttggcga
     1561 cggctatatc gacacggtca gcgcggtttc ggcggttttc gagcgcaggc aggggcttga
     1621 ctgggcgttc gtcgtctatg cggctttggc ggttttcctg cttgtcgtgc tgagcgtgcg
     1681 gctgtcgctg atgtacgcgg ccagcgtggc ggagcggcgc atcgtgacgc tgaacgcgct
     1741 cagcctcagc gaaggcgccg gactgaaact gctgatcggg acggtgatcg tactcttgcc
     1801 gctggcgctg gcgatgggcc ttgtccatgt tctgcgcggc gtcgcgcatc cggcggcggc
     1861 cctgcacacg gtcctgtgcg ccgtcgcgat tttgctggtc ctgccgctat cggcgggatt
     1921 tttcgcatcg gcctataggc agatacggga caaccgggct aaatagtcga atccgcaaca
     1981 agccggcgcg ccgggggccg aggatttgac catacgcatg accctgtctt tcgactttgc
     2041 tttcgagggt tttcgcctca tccggcaaag accgaaactg atcctgttct gggggctttt
     2101 atccctgatc ggcaacagcg cgataacggc gattctggcc ggtatggccg gtccggccct
     2161 ggccagcctc gcgcatatgc aggggcaggc gccggaccag caaaccgctc tgtcgctgat
     2221 gtcgcggatc atgccggcga tgatgaccag cctgcccatc tatgtcctga tgtccgccgt
     2281 gctgagcgcc gcgatctgcc gggcggtcgg cggcgacagc gacgaccgtt tcggctttct
     2341 gggcttcggc aagcgcgaat gtctgctgat cgcgctggag atcgtcatgc tgctgctgcg
     2401 cgccgtcgcg atgaccggct tcctcgtgtt cggcgaactg gcgtcgcaga tcctgcccgg
     2461 cccggcgggc gaggcgcttt tgagcctgtt cagccttgcg ggcatgggcg tggcgctatg
     2521 gctgagcgtg cggttgtcgc tgaacacggc ccagaccttc gacgaaggcc gcctcaatat
     2581 atggggcagt ttcgagctga gccgcgaacg gttctggacg ctgctgatcg gctatgcgac
     2641 cgccttcggc ctggcgctgg tggtgatgtt cctatgcaag cagatcatcg acggcgcgct
     2701 ggtgatcgcc tccggcatga cctcggccaa gggatcggac gcgctcgacc tgagcagcc
//
LOCUS       DASVLL010000408         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_295790,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000408 DASVLL010000000
VERSION     DASVLL010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295790"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VF402_06790"
     CDS             complement(<1..1038)
                     /locus_tag="VF402_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7800038.1"
                     /translation="MSGSATTADATSVQPNPKHVQLQGMTLWLCGVLLALANFVAILD
                     VSIANVSVPNIAGALGVSTSQGTWVITSYAVAEAISVPLTGWLAARFGTVRVFSMALI
                     GFGLASLLCGMSPTFHILIMARVIQGFCGGPLMPLSQTLLLTIFPKKLQPAAMAIWAI
                     TTLVGPIAGPVLGGTLCDNLGWGSIFLVNVPIAILAGFLVWRVLFSQETQTVKARVDV
                     VGLLLLILWVGALQIMLDLGKDRDWFASPLILGLAVTAALGFIAFMIWELTDAHPIVD
                     LHVFRHPGFSLSMICLALMIGSFFAINVIAPLWMQTNLGWTATSAGNATGLIGILAII
                     AAPIASQLTTKV"
     gene            complement(1108..>1987)
                     /locus_tag="VF402_06795"
     CDS             complement(1108..>1987)
                     /locus_tag="VF402_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7800039.1"
                     /translation="QVDIYDTKPVKKGDVLLTLDDTDARLALASAQAQLDQVSRQVST
                     IYENDATYAAQVQAAQAQVASAQAALDKAQKAYDDRKTLVSIGAVTGEDLTSAKAALD
                     QARAALNNAQAQAAAAQGQRASNNALISNVSAGDNPQVVAARVKVQQAQVDLDRTVIR
                     APIDGVVAKNTVEVGQRVQPGTPLMDVVPIQSAYVNANFKEVQLKKVRIGQPVTLTAD
                     VYGGAVKFHGKVVGLSGGTGSAFSVIPAQNATGNWIKVVQRLPVRIALDPKELQQHPL
                     RVGMSMDAKIDVSGAK"
BASE COUNT          346 a          615 c          713 g          313 t
ORIGIN      
        1 gaccttggtg gtcagttgcg aggcgatggg cgcggcgatg atggccaaaa tcccgatcag
       61 gccggtggcg ttgcccgccg aggtggcggt ccagccgaga ttggtctgca tccacaaagg
      121 cgcgatgacg ttgatggcga agaaggagcc gatcatcagc gccagacaga tcatcgacaa
      181 agaaaagccg ggatggcgga agacgtgcag gtcgacgatc ggatgcgcgt cggtcagctc
      241 ccagatcatg aaggcgatga agccgagcgc ggcggtcacg gccaggccaa ggatcagcgg
      301 cgaggcgaac cagtcgcggt ccttgccgag gtcgagcatg atctgcaacg cgccgaccca
      361 caggatcagc agcaacaggc cgaccacgtc gacgcgcgcc ttgacggtct gggtttcctg
      421 actgaacagc acgcgccaga ccagaaaacc ggccaggatg gcgatcggca cgttgacgag
      481 gaagatcgaa ccccagccga gattgtcgca cagcgtcccg cccagcaccg gcccggcgat
      541 cggcccgacc agcgtggtga tggcccagat cgccatcgcc gccggttgca gcttcttcgg
      601 gaagatggtc agcagcaggg tctgcgacag cggcatcagc ggcccgccgc agaagccctg
      661 aatcacgcgc gccatgatca ggatgtggaa ggtcggcgac atgccgcaca gcagcgaggc
      721 gaggccgaag ccgatcagcg ccatcgaaaa gacgcgcacc gtgccgaagc gcgccgccag
      781 ccagccggtc agcggcaccg agatcgcctc ggccaccgcg taggaggtga tgacccatgt
      841 gccctgcgag gtcgagacgc cgagcgcccc ggcgatgttg ggcaccgaga cgttggcgat
      901 ggagacgtcg agaatggcga cgaagttggc cagcgccaga aggacgccgc acagccacag
      961 ggtcatgccc tgaagctgga cgtgtttggg gttgggctgg acgctggtgg cgtcagccgt
     1021 ggtcgcagaa ccggacatgg tctggctccc tgatgaagga tgacaatcga aaaaggccgc
     1081 cgccggagcg ggcggccttt caaaggctta tttcgcgccc gagacgtcga tcttggcgtc
     1141 catgctcatg ccgacgcgca acgggtgctg ttgcagttcc ttcgggtcga gcgcgatgcg
     1201 cacgggcagg cgctgcacga ccttgatcca gttgccggtg gcgttctggg ccgggatgac
     1261 cgagaaggcc gagccggtgc cgccggacag gccgaccacc ttgccgtgga acttgaccgc
     1321 gccgccatag acgtcggcgg tcagggtcac cggctggccg atgcggacct ttttaagctg
     1381 cacttccttg aagttggcgt tcacataggc gctctggatc ggcaccacgt ccatcagggg
     1441 cgtgccgggc tggacgcgct ggccgacctc gaccgtgttc ttggcgacga cgccgtcgat
     1501 gggggcgcgg atgacggtgc ggtccagatc gacctgcgcc tgctgcacct tgacgcgggc
     1561 ggcgacgacc tgcgggttgt cgccggcgct gacattggag atcagcgcgt tgttggaggc
     1621 gcgctggccc tgcgcggcgg cggcctgggc ctgcgcgttg ttgagcgcgg ccctggcctg
     1681 atccagcgcg gccttggccg aggtcagatc ctcgccggtg acggcgccga tggagaccag
     1741 cgtcttgcgg tcgtcatagg ccttctgcgc cttgtcgagc gcggcctgcg ccgaggcgac
     1801 ctgcgcctgc gcggcctgca cctgcgcggc gtaggtggcg tcgttttcgt agatggtcga
     1861 gacctgacgg ctgacctgat cgagctgggc ctgcgccgag gcgagggcca gcctggcgtc
     1921 ggtgtcgtcg agggtcagca gcacgtcgcc cttcttcacc ggcttggtgt cgtagatgtc
     1981 gacctgc
//
LOCUS       DASVLL010000409         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_134670,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000409 DASVLL010000000
VERSION     DASVLL010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134670"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VF402_06800"
     CDS             <1..723
                     /locus_tag="VF402_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7800040.1"
                     /translation="AASLALSLSACDSRNLSESFAAIKAIKVDDGADPNAPKDHKQLD
                     GQDLKLVVGTLKALTVDKGGLLAPKGEANGDAKPHRLDIDVVDPLKMPPPDDGGPLRV
                     LPADIAAAARLQQAAHDLPDLPIQTLTAAVSGKSAPVQAAPVQPAQVQAAQAQPAPAE
                     TATRLIQIGSFSSIGAARQAWDDLQERYPVALSYKARFQPITTADGRAMVRLKVGPVA
                     DARAEALCQALSVRDNWCAKAG"
     gene            818..2482
                     /locus_tag="VF402_06805"
     CDS             818..2482
                     /locus_tag="VF402_06805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7800041.1"
                     /translation="MTRELSVQHILFDSATRLIPQVFGALMFIAGALLLATVLTPIDP
                     TFMPYVKTWLPLGLVEFSHAIGAAVGVLMLFVARGLWERIDTAWYMALFLLVLGAVAS
                     VTRELAMWPAVGLTICALLLVPCKDAFNRRSNLLSLSFNPVWVVASLATLAAVVWFGF
                     FFYKHVPYAHSMWTHFSYRASASRFLRALVVMAVTVAILLLYRLFGIARQLPPEPDEA
                     DMARLRQVVAGASNPQAWLALLRDKHILWNDDQSAFLMYGVSGNQWVVMGEPVGDRIE
                     AEALCWRLKEMASLSNAKLSFYQVSPRLLPLMIDLGLVPYKIGEEALVDVRSFDLAGK
                     KGYGLRQVVRRFETLGATFEVIAPEALEPHMERLKEVSDAWLKAKSGKEKGYSLGSFQ
                     ADYMRLMPVGVLRMDGRIMAFANLWPTHDRTLLALDLMRYDPASPHNTMDYLFVQLIH
                     YARDNGYTYFSLGLAPLGGLQARPLSNAWPKLAALIWEIGGEFYNFEGLRAYKEKFRP
                     QWEPRYFAVPGLETALVPALMAVAALGGKAPRAARQNADQDSDPAD"
BASE COUNT          443 a          868 c          946 g          490 t
ORIGIN      
        1 gccgcaagtc tggcgctgag cctgagcgcc tgcgacagcc gcaatctgag cgaaagcttc
       61 gcggccatca aggcgatcaa ggtggatgac ggcgccgatc cgaacgcgcc caaggaccat
      121 aagcaactcg acggtcagga tctcaaactg gtcgtcggca cgcttaaggc gctgacggtc
      181 gacaaggggg ggctgctggc gccgaagggt gaggcgaacg gcgatgcgaa gccgcatcgt
      241 ctggacatcg acgtggtcga tccgctgaaa atgccgccgc cggacgatgg cggcccgttg
      301 cgcgtcctgc ccgccgacat cgccgccgcc gcgcgcctgc aacaggcggc gcacgatctg
      361 cccgacctgc cgattcagac cctgaccgcc gccgtttcgg gtaagtccgc cccggttcag
      421 gccgctcccg ttcagcctgc gcaggttcag gcggctcagg cccagcccgc ccccgccgaa
      481 acggcgaccc ggctgattca gatcggctcc ttctccagca tcggggccgc gcgtcaggcg
      541 tgggacgatc tgcaggaacg ctatcccgtc gccctgagct acaaggcgcg tttccagccg
      601 atcaccaccg ccgacggccg cgccatggtg cggctcaagg tcgggccggt ggcggacgcg
      661 cgcgccgagg ccctgtgcca ggcgctaagc gtgcgcgaca attggtgcgc caaagccggt
      721 taagcgcgta tccggcctga ttttcccgga ctttgcctgt atagtgcgaa tcgctggcca
      781 aagcgggtca ggggtgcagg gagccgcgaa ccggcgcatg acacgcgaac tgagcgttca
      841 gcatattctg ttcgattcgg ccacgcgcct gattcctcag gtcttcggcg ccctgatgtt
      901 catcgcgggc gccctgctgc tggcgacggt gctgacgccg atcgacccga ccttcatgcc
      961 ctatgtgaag acctggctgc ctctggggct ggtcgaattc tcgcacgcca tcggggcggc
     1021 ggtcggggtg ctgatgctgt tcgtcgcgcg cggcttgtgg gagcgcatcg atacggcgtg
     1081 gtacatggcg ctgttcctgc tggtgctggg cgccgtggcc tcggtcacgc gcgagctggc
     1141 gatgtggccc gccgtggggc tgacgatctg cgccctgctg ctggtgccgt gcaaggacgc
     1201 cttcaaccgc cgctccaacc tgctgagcct gtcgttcaat ccggtgtggg tcgtcgccag
     1261 tctggccacg ctggccgccg tcgtctggtt cggcttcttc ttctacaagc acgtgcccta
     1321 cgcccattcg atgtggacgc atttctcgta ccgcgcctcg gcctcgcgct tcctgcgcgc
     1381 gctggtggtc atggcggtga cggtggcgat cctgttgctg taccgcctgt tcggcatcgc
     1441 gcgccagttg ccgccggagc cggacgaggc cgacatggcg cggctcaggc aggtcgtggc
     1501 cggagcctcc aacccgcagg cgtggctggc cctgctgcgc gacaagcata tcctgtggaa
     1561 cgacgatcag agcgcgtttc tcatgtacgg cgtctccggc aatcagtggg tggtgatggg
     1621 cgagcctgtc ggcgacagga tcgaagccga ggcgctgtgc tggcggctga aggagatggc
     1681 ctcgctgagc aacgcgaaac tcagcttcta tcaggtcagc ccgcgcctgc tgccgctgat
     1741 gatcgatctg ggcctcgtgc cttacaagat cggcgaggag gcgctggtcg acgtgcgaag
     1801 cttcgacctg gccgggaaaa agggctacgg cctgcgtcag gtcgtgcgcc gtttcgagac
     1861 gctgggcgcg acgttcgagg tgatcgcgcc ggaggctctt gagccgcata tggagcggtt
     1921 gaaagaggtg tccgacgcct ggctcaaggc caagtccggc aaggaaaagg gctattcgct
     1981 gggcagtttt caggcggatt acatgcggct gatgccggtc ggggtgctgc gcatggacgg
     2041 ccgcatcatg gccttcgcca acctgtggcc gacgcatgac cggaccttgc tggcgctgga
     2101 tctgatgcgt tacgatccgg cctcgccgca caatacgatg gactatctgt tcgtgcagtt
     2161 gatccattac gcgcgcgaca acggctatac ctatttctcg ctggggctgg cgccgctggg
     2221 cgggttgcag gcgcggcccc tgtccaacgc gtggcccaag ctggcggcgc tgatctggga
     2281 gatcggcggc gagttctata atttcgaggg cctgcgcgcc tacaaggaaa agttccgccc
     2341 gcagtgggag ccgcgctatt tcgccgtgcc ggggctggag acggcgctgg ttccggcgct
     2401 gatggcggtg gcggcgctgg gcggcaaggc gccgagggcg gcgcggcaaa acgccgatca
     2461 ggacagcgac ccggcggatt agagcgcttt ccgaaaagtg tgacgcactt ttcggattaa
     2521 aaagcgcgta acagcataaa gttagagcgc cgatctgatg caatcagatc ggaaaacgct
     2581 ctaaaggacc ggaatctcgc gcgcgaaggt caggccctgc ggcgagacgc aaaagctgaa
     2641 cgccagcatt tcgacgcccg cctgtcgcgc ggcgtcgaag gcgcgcccga aggccggatc
     2701 gcagtcgcgc gccacgtcga acgcggtcac gtcgtcgcgc tggatgc
//
LOCUS       DASVLL010000410         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_401849,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000410 DASVLL010000000
VERSION     DASVLL010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401849"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="VF402_06810"
     CDS             <1..1674
                     /locus_tag="VF402_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7800042.1"
                     /translation="GVNGIIESLRKHKDDIRFIQTRHEEAAAFMACAYAKTTGRLGAC
                     IATSGPGGIHLLNGLYDAKLDGAPVIAITGMQFHDLLHTHTQQDVELDKLYMDVCVYN
                     CRIMGPAHTENVVELACRTALTRRGVAHVTMPVDMQSEAVDKDARSKRNVARHVSGLP
                     AFGAGLPHEDDLQRAADLLNAGRKTVIMSGAGAIGAGPLVEAVADRLGAVIVKPLLGK
                     ASVPDASDYCLGGVGLLGTLPSQLALEECDTLLIVGSSFPYIEFYPKPGHAKCVQIDA
                     DGARIGLRCPVDAGLVGDATACLERLLPRLEARADRKFLELGRRRKADWLELLEKRGT
                     ADVMPMKPQVVAYELGKRLADDAIFISDSGTIATWYARYLPFRYGQHCTLSGNLATMA
                     CGLPYAIAAQVAHPGRQVVAMVGDGGFSMLMAEFATAVKYRLPIKIVLIKNNALGQIK
                     WEQMVFLGNPEYECELQPIDFCKIAEACGGKAVRIEDPARCGAQLDAALAEPGPVLIE
                     AVVDPNEPPMPPKVSFEDMKKFSEALAKGTLHRGDIIRTIFRDKVRELV"
     gene            1674..>3247
                     /locus_tag="VF402_06815"
     CDS             1674..>3247
                     /locus_tag="VF402_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7800043.1"
                     /translation="MADAAHGRDPLAQALRGAIDGEVRFGDGDRALYAADASNFREVP
                     RGVVIPRSMDEMARIIALCARHGAAVVHRGGGTSLAGQTVSAGAVVIDSSKYCNRILD
                     IDPQARLARVEPGVVLDDLRAAAGRHGLTFGPDPATHSHNTLGGMLGNNSCGVHSLYA
                     RRTVDNVRELEALTYDGLRLTLGATPDDVLRRKLDLPGREGGIYRDLKALRDAYADDI
                     RRRYPDIPRRVSGYSLDELLPERGFQVARALVGSESTCVTILSATLELVPDPPCKALL
                     VIGFDDLDHAADAVPDLLRFRQGREGRGLIALEGMDADLTRAMRAKNFRVEDLRYLPG
                     GEGWLLAQFGGDTPDEAAAHARACRRSLERHPHVAGMRLLTDPEARDRLWKVREAGLG
                     ATSWVEGQADTWPGWEDSAVAPERLGAYMRDLRALYAKYGYQGAMYGHFGDGLIHSRV
                     SFGLHTQEEVADYRRFLEEAAVLVTRHGGSLSGEHGDGRQRAELLPVMYGERLIAAFA
                     AFKRIWDPLGRMNPGR"
BASE COUNT          502 a         1077 c         1155 g          513 t
ORIGIN      
        1 ggggtcaacg gcatcatcga aagcctgcgc aagcataagg acgacatccg cttcatccag
       61 acccgccatg aggaggcggc ggccttcatg gcctgcgcct acgccaagac gacgggccgc
      121 ctgggcgcgt gcatcgccac ctccggtccc ggcggcattc atctgctgaa cggactttac
      181 gacgccaagc tggacggcgc gcccgtcatc gcgatcaccg gcatgcagtt tcacgacctg
      241 ttgcacaccc atacgcagca ggacgtcgag ctggacaagc tctacatgga cgtctgcgtc
      301 tataactgcc gcatcatggg gccggcgcat accgagaacg tggtggagct ggcctgccgc
      361 acggcgttga cgcggcgcgg ggtcgcccac gtcaccatgc cggtcgacat gcagtccgaa
      421 gcggtcgaca aggacgcgcg ctcgaagcgc aacgtcgcgc gtcacgtctc gggcctgccc
      481 gccttcggcg ccggactgcc gcacgaggac gacctgcaac gggccgccga cctgctcaat
      541 gcgggccgca agaccgtcat catgagcggc gcgggcgcca tcggggccgg accgctggtc
      601 gaggcggtcg ccgaccggct gggcgcggtg atcgtcaagc cgctgctggg caaggcttcg
      661 gtgccggacg cgtcggatta ctgcctcggc ggcgtcgggc tgctgggcac gctgccgtcg
      721 caactggcgc tggaggaatg cgacacgctg ctgatcgtcg gctccagctt tccctatatc
      781 gaattctacc ctaaacccgg ccacgccaaa tgcgtgcaga tcgacgccga cggcgcgcgc
      841 atcgggctgc gctgtccggt ggatgccggg ctggtcggcg acgcgacggc gtgtctcgaa
      901 cggctcttgc cgcgccttga ggcgcgcgcg gatcgcaaat tcctcgaact ggggcgcagg
      961 cgcaaggcgg actggctgga gctgctggaa aagcgcggca cggccgacgt catgccgatg
     1021 aagccgcagg tcgtggccta tgaactgggc aagcggctgg cggacgacgc catcttcatc
     1081 tccgattccg gcacgatcgc gacctggtac gcgcgctatc tgccgttccg ctacggccag
     1141 cattgcaccc tgtccggcaa tctggcgacc atggcctgcg gcctgcctta cgccatcgcc
     1201 gcgcaggtcg cccatccggg ccgtcaggtg gtggcgatgg tcggcgacgg cggcttctcg
     1261 atgctgatgg ccgagttcgc gacggcggtg aaataccggc tgccgatcaa gatcgtgctg
     1321 atcaagaaca acgcgctggg ccagatcaag tgggagcaga tggtctttct gggcaatccc
     1381 gaatacgaat gcgaattgca gccgatcgac ttctgcaaga tcgccgaggc gtgcggcggc
     1441 aaggcggtgc gcatcgaaga ccccgcgcgt tgcggcgcgc agctcgacgc cgctttggcc
     1501 gagccggggc cggtgctgat cgaggccgtg gtcgatccca acgagccgcc gatgccgccc
     1561 aaggtcagct tcgaggacat gaagaagttt tccgaggcgc tggccaaggg cacgctgcat
     1621 cgcggcgaca tcatcaggac gatcttcagg gacaaggtgc gggagctggt ctgatggcgg
     1681 acgcggcgca cgggcgcgat ccgctggcgc aggccctgcg cggcgccatc gacggcgagg
     1741 tgcgcttcgg cgacggcgac cgcgcgctgt acgcggcgga cgcctccaac ttccgcgagg
     1801 ttccgcgcgg cgtggtcatc cccagaagca tggacgagat ggcgcgcatt atcgcgctgt
     1861 gcgcccgcca cggcgccgcc gtcgtccatc gcggcggcgg cacgtctttg gccggacaga
     1921 cggtgtccgc cggggcggtg gtcatcgatt cctcgaaata ctgcaaccgc atcctcgaca
     1981 tcgatccgca ggcgcggctg gcgcgcgtcg agccgggcgt ggtgctggac gacctgcgcg
     2041 cggcggcggg ccggcacggc ctgaccttcg gccccgatcc ggcgacgcac agccacaaca
     2101 cgctgggcgg catgctgggc aacaattcct gcggcgtgca ttcgctctat gcgcgccgca
     2161 cggtcgataa tgtgcgcgaa ctggaggcgc tgacctatga cggcctcagg ctgaccctgg
     2221 gcgccacgcc ggacgacgtt ttgcggcgca agctggacct gccggggcgc gagggcggga
     2281 tttaccgcga tctgaaggcc ctgcgcgacg cctatgccga cgacatccgc cggcgttatc
     2341 ccgacattcc gcgccgcgtc tccggctata gtctcgacga gttgctgccc gagcgcggct
     2401 ttcaggtcgc gcgcgcgctg gtcggcagcg aatcgacctg cgtgaccatt ctgtcggcga
     2461 cgctggagct ggttcccgat ccgccgtgca aggcgttgct ggtgatcggg ttcgatgatc
     2521 tcgaccacgc cgccgacgcc gtgccggacc tgttgcggtt ccggcagggg cgcgagggcc
     2581 ggggcctgat cgcgctggag ggcatggacg ccgatctgac ccgcgccatg cgggccaaga
     2641 atttccgcgt cgaggacctg cgctacctgc ccggcggcga gggctggctg ctggcgcagt
     2701 tcggcggcga cacgccggac gaggccgccg cgcatgcgcg ggcctgccgc cggtctctgg
     2761 agcgccatcc gcatgtcgcg ggaatgcgcc tgctgaccga tcccgaagcg cgcgaccggc
     2821 tgtggaaggt gcgcgaggcc gggctgggcg cgacctcgtg ggtcgaggga caggccgata
     2881 cctggccggg ctgggaggat tccgccgtcg cgccggagcg gctgggcgcc tatatgcgcg
     2941 acctgcgcgc cctgtacgcg aaatacggct atcagggcgc gatgtacggc catttcggcg
     3001 acggcctgat ccattcgcgc gtttcgttcg gcttgcacac gcaggaagaa gtggccgatt
     3061 atcgccgctt cctcgaggag gccgccgttc tggtgacgcg gcacggcggc agcctttcgg
     3121 gcgagcatgg cgacggccgc cagcgggccg aactgctgcc ggtgatgtac ggcgaaaggc
     3181 tgatcgcggc cttcgccgcg ttcaagcgca tctgggatcc gctggggcgg atgaatccgg
     3241 gccggat
//
LOCUS       DASVLL010000411         4353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_511855,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000411 DASVLL010000000
VERSION     DASVLL010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511855"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VF402_06820"
     CDS             complement(<1..901)
                     /locus_tag="VF402_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7800044.1"
                     /translation="MLLQNEHDLVVLLGAAAVILLVLSIMGGVIAALKAFQVRQFQDL
                     LFKAERKIDTLERRMFNVLNAVPVALVETDATGKFTFANRAAHQLLGRKDNELLGLRF
                     HSATWGITYPDGRVIPADLLPIARTLRGQTVRGFQHMLTRYGAREKILVSVTSMPVMN
                     SVGEVIGSSAAFVELETTQGEGIDDLNGLWRGHWFEAATAPFWGLDAAGRIVDLNAAA
                     CEAFGVTREQALGGGWTQAFVADADFQAAMDYLAQVQEGGAHDLSAGLTLRAPLTGET
                     APMRVTAWRVRTHEGGAEGLTVMA"
     gene            1134..2180
                     /gene="obgE"
                     /locus_tag="VF402_06825"
     CDS             1134..2180
                     /gene="obgE"
                     /locus_tag="VF402_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX7800045.1"
                     /translation="MKFLDQCKIYIRSGNGGAGSVSFRREKFIPNGGPDGGDGGKGGD
                     VWIEAVEGLNTLIDYRYQQHFKARTGEHGKGRQMHGANAEDVVLRVPVGTEVLDEEQE
                     TVIVDLDEAGKRFLLAKGGNGGWGNTRFKGPVNQAPTFALPGQEGEERWIWLRLKLIA
                     DAGLVGLPNAGKSTFLAASSAARPKIADYPFTTLTPNLGVIDLGPEQRFVLADIPGLI
                     EGASEGAGLGTRFLGHVERNKVLIHLIDGAQDDPVEAYRVIRHELEAYDEELAKKPEI
                     VAVNKCDALDAEARKKVARAIKKASGQTPHLISGVTGEGVRELLFAALAAIEAGRAAP
                     KAAPSASYNPWDNA"
     gene            2186..2827
                     /locus_tag="VF402_06830"
     CDS             2186..2827
                     /locus_tag="VF402_06830"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX7800046.1"
                     /translation="MWFAGPAPMPPAVRAGGVRPGFHLEPGMKIGLFGGSFNPAHEGH
                     RHVADTARVRLGLDRIIWLVSPQNPLKSRDETAPLGTRIAQIAPFIGPKDIVSDFETR
                     IGADYTLDTIRALKARFPGVRFVWIMGGDSLAGFHRWRGWAQLMHMVPMAVVSRPGAL
                     MKARLSPAAQRFARFRKPERAARALAFQPAPAWICLKGPLHGHSSTAIRKGRT"
     gene            3018..3353
                     /gene="rsfS"
                     /locus_tag="VF402_06835"
     CDS             3018..3353
                     /gene="rsfS"
                     /locus_tag="VF402_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX7800047.1"
                     /translation="MEPLERLIVERLDDDKAQDIVCIDLKGKSSVADTLIIASGRSHR
                     HVGALADHVLRALKDAGYGKAKVEGLPACDWVLIDVGDVVVHLFRPEVRSFYNIEKIW
                     SLSPEGAPA"
     gene            3358..3864
                     /locus_tag="VF402_06840"
     CDS             3358..3864
                     /locus_tag="VF402_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX7800048.1"
                     /translation="MKLTLCAVGKLGATVENALARDYLGRAGQTGRGLGVSAVELIEV
                     DARKAAKAQTSALLKAQEADAIRDALGLKGGGEPGILIACDEHGEALTSRQIARRIEG
                     YKDRGERRVTFLIGGADGLDPSLLQTAAFSLAFGPQTWPHALARVMLAEQMYRSLSIL
                     AGSPYHRD"
     gene            3963..>4353
                     /locus_tag="VF402_06845"
     CDS             3963..>4353
                     /locus_tag="VF402_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7800049.1"
                     /translation="MTAAPKILPTDWPVRRFRAALFDMDGTIVNSIAAAERVWGAWAQ
                     AHGLDVAAFLPTIHGKRSQDTIAALNLPGIDPEAEARRINRIEIDTVDGVAAIAGADA
                     FLRGLPADRWAVVTSAPRALALARIAAA"
BASE COUNT          708 a         1444 c         1515 g          686 t
ORIGIN      
        1 gggccattac ggtcaggccc tcggcgccgc cctcatgggt gcgcacgcgc cacgcggtca
       61 cgcgcatcgg cgcggtctcg ccggtcaggg gcgcgcgtaa ggtgaggccg gccgacaggt
      121 cgtgcgcgcc gccttcctgc acctgagcca gatagtccat cgcggcctga aaatccgcgt
      181 cggcgacgaa ggcctgcgtc cagccgccgc ccagcgcctg ttcgcgcgtc acgccgaagg
      241 cttcgcaggc ggcggcgttg agatcgacga tccggcccgc ggcgtccagc ccccagaagg
      301 gcgcggtggc cgcctcgaac cagtggccgc gccacaggcc gttgaggtcg tctatgccct
      361 cgccctgcgt ggtctccagc tcgacgaagg cggcgctgga gccgatgacc tcgccgacgc
      421 tgttcatcac cggcatggag gtgaccgaga cgaggatctt ctcgcgcgcg ccgtagcgcg
      481 tcagcatgtg ctggaacccc ctgacggtct ggccgcgcag ggtgcgcgcg atcggcagca
      541 ggtcggccgg gatcacgcgc ccgtcgggat aggtgatgcc ccaggtcgcc gaatggaagc
      601 gcaggcccag aagctcgttg tccttgcggc ccagaagctg atgcgcggcg cggttggcga
      661 aggtgaactt gccggtggcg tcggtttcga ccagcgcgac cggcacggcg ttcagcacgt
      721 tgaacatgcg ccgctccagc gtgtcgatct tgcgctcggc cttgaacaaa agatcctgaa
      781 actggcgcac ctgaaaggct ttcagcgccg cgatcacccc gcccatgatc gacaggacga
      841 gcaggatgac ggcggctgcg cccagcagca ccacgagatc gtgctcgttc tgcaataaca
      901 tgccccgcac cccttcgtgc atcgcgccgg agggaaggcg cgccgcgttc gcaaagactc
      961 taatgatctg gtcgtggcgc gtcatctgcc tcgttttgcg acagactccg ccccctggcc
     1021 cggcgcgcag caaaaaacat acccgccggg tctgcaagcg gattctcgcg catggcaagg
     1081 ccgcgaccgt ggcgtcgctt gccatgaggt gtgttttcgt tcaaagaagc gcgatgaaat
     1141 tcctcgatca gtgcaaaatc tacatccgct caggcaacgg cggcgcgggg tccgtgtcgt
     1201 tccggcggga gaagttcatc ccgaacggcg ggccggacgg cggcgacggc ggcaagggcg
     1261 gcgacgtgtg gatcgaggcg gtcgaggggc tgaacacgct gatcgactat cgttaccagc
     1321 agcatttcaa ggccaggacc ggcgagcacg gcaaggggcg ccagatgcac ggcgccaacg
     1381 ccgaggacgt ggtgctgcgc gtgccggtcg gcaccgaggt gctggacgag gagcaggaga
     1441 cggtgatcgt cgatctcgac gaggcgggca agcgcttcct gctggccaaa ggcggcaacg
     1501 gcggttgggg caatacccgc ttcaaggggc cggtcaatca ggcgccgacc ttcgccctgc
     1561 cggggcagga gggcgaggag cgctggatct ggctgcggct gaagctgatc gccgatgcgg
     1621 ggctggtcgg attgcccaat gcgggcaagt cgacctttct ggccgcgtcc tccgccgcgc
     1681 ggcccaagat cgccgattat ccgttcacca ccctgacgcc gaatctcggc gtgatcgatc
     1741 tggggccgga gcagcgtttc gtgctggccg acattccggg cctgatcgag ggcgcctcgg
     1801 aaggcgccgg gctgggcacg cgctttctag gtcatgtcga gcgcaacaag gtgctgatcc
     1861 acctgatcga cggcgcgcag gacgatccgg tcgaggcgta tcgcgtcatc cggcatgagc
     1921 ttgaggctta cgatgaggaa ctggcgaaaa agccggaaat cgtcgcggtc aacaagtgcg
     1981 acgcgctgga cgccgaggcg cgcaaaaagg tcgccagggc gatcaagaag gcgtcgggtc
     2041 agacgccgca tctgatttcc ggcgtgaccg gcgaaggggt gcgcgagttg ctgttcgccg
     2101 cgctggcggc catcgaggcg ggccgcgccg cgccgaaggc ggcgccgtcc gcaagctata
     2161 atccgtggga caacgcttaa aggccatgtg gttcgccggt cccgccccga tgccgcccgc
     2221 cgtgcgtgcg ggcggagtgc gtcccggctt tcaccttgag ccgggcatga agatcggcct
     2281 gttcggcggc tcgttcaatc cggcgcacga aggccaccgc cacgtcgcgg acaccgcgcg
     2341 cgtcaggctg gggctggacc gcatcatctg gctggtctcg ccgcagaacc ccttgaaaag
     2401 ccgcgacgag acggcgccgc tggggacgcg catcgcgcag atcgcgccgt tcatcgggcc
     2461 gaaggacatc gtctccgatt tcgagacgcg catcggcgcc gactatacgc tggatacgat
     2521 ccgggcgttg aaggcgcgct ttccgggcgt gcgtttcgtc tggatcatgg gcggggactc
     2581 gctggcgggc tttcaccgct ggcgcggctg ggcgcagttg atgcacatgg tcccgatggc
     2641 ggtggtgtcg cggcccggcg cgctgatgaa ggcgcgcctg tcgcccgccg cccaacgctt
     2701 cgcccgcttc cgcaagcccg aacgcgccgc gcgggcgctg gcgttccagc ccgctccggc
     2761 gtggatctgt ctgaaagggc cgctgcacgg ccattcctcg acggccatcc gcaaggggcg
     2821 gacttgagag ggcggctata aatttcatca tcggcggatg accggacggg ctttctgtgc
     2881 tagattgtct gtcatccgtg acaaaaagga acctgacact gtctccccaa aacgcgcaga
     2941 gcgcgcctga aaccccggct ccggtcgccc tttccgacgt cgagccgcaa agtaccggct
     3001 tcgccggcga cgaaacgctg gagccgcttg agcgcctgat cgtcgagcgc ctcgacgatg
     3061 acaaggcgca ggacatcgtc tgcatcgacc tgaagggcaa gtcctcggtc gccgacaccc
     3121 tgatcatcgc ctccggacgt tcccaccgcc atgtcggcgc gctggccgac catgtgctgc
     3181 gcgcgctgaa ggacgccggt tacggcaagg ccaaggtcga ggggctgccc gcctgcgact
     3241 gggtgctgat cgatgtcggc gacgtggtgg tccacctgtt ccgtcccgaa gtgcgcagct
     3301 tctataatat cgagaaaatc tggtcgctgt cgccggaagg cgcgccggcc tgatcgcgtg
     3361 aaactcaccc tctgcgccgt cggcaagctg ggcgcgacgg tcgagaacgc gctcgccagg
     3421 gactatctgg gccgcgccgg gcaaaccggg cgcggccttg gcgtgtccgc cgtcgagctg
     3481 atcgaggtcg acgcgcgcaa ggcggcgaag gcgcagacct cggcgctgct caaggcgcag
     3541 gaggccgacg ccattcgcga cgcattaggg cttaaaggtg ggggcgagcc gggcattctg
     3601 atcgcctgcg acgagcacgg cgaggcgctg acctcgcgcc agatcgcgcg ccgcatcgaa
     3661 ggctataagg accggggcga gcgccgcgtg accttcctga tcggcggggc ggacggactc
     3721 gatccgtcgc tgttgcaaac ggcggccttt tcgctggcct tcgggccgca gacctggccg
     3781 cacgcgctgg cgcgcgtcat gctggccgaa cagatgtacc ggagcttgag catcctggcc
     3841 ggttcgccct atcatcgcga ctgatcaccg ccggccggta ttttgtaatg caaaatgaat
     3901 tgtgcgccgc agcataatcg gcttgaatcg ggcgtctgca cgcagccctg aaggacatcc
     3961 ccgtgaccgc cgcgcccaaa atcctgccga ccgactggcc cgtgcgccgc tttcgcgccg
     4021 cgctgttcga catggacggc accatcgtca actccatcgc cgccgccgag cgcgtctggg
     4081 gcgcgtgggc gcaggcgcac gggctggacg tcgccgcctt cctgccgacc atccacggca
     4141 agcgctcgca ggacaccatc gcggcgctga acctgccggg gatcgacccg gaggccgagg
     4201 cgcggcgcat caaccggatc gagatcgaca cggtggacgg cgtcgccgcc attgccgggg
     4261 cggacgcctt cctgcgcggg ctgcccgccg accgctgggc ggtggtgacc tcggcgccgc
     4321 gcgcgctggc tctggcgcgc atcgccgccg ccg
//
LOCUS       DASVLL010000412         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_26650, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000412 DASVLL010000000
VERSION     DASVLL010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26650"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VF402_06850"
     CDS             complement(<1..607)
                     /locus_tag="VF402_06850"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEX7800050.1"
                     /translation="MNRRGVIAALPALALTGCQAAREAVGGRTAVFLRAARAQMRGWT
                     SYDARYTPIAYPGGDVRPDRGVCADVIVRAYRGLGIDLQQRVHEGMLAHFDLYPKLWG
                     LTAPDPNIDHRRVPNLAVFFSRFGKILPISRDGADYRPGDILVTRPIGRPHISIVSDR
                     RAWLGAGDLMVIQNMGMSVQENNDLLRYAITGHFRYGLDQPA"
     gene            complement(609..734)
                     /locus_tag="VF402_06855"
     CDS             complement(609..734)
                     /locus_tag="VF402_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX7800051.1"
                     /translation="MKRLVIVSLLALAPLLAACHTIQGVGQDITAAGKGLERATK"
     gene            complement(873..1001)
                     /locus_tag="VF402_06860"
     CDS             complement(873..1001)
                     /locus_tag="VF402_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX7800052.1"
                     /translation="MKRIVLISVMALLPLLAACHTVQGAGKDISAVGHGVSDAASH"
     gene            1162..>1887
                     /locus_tag="VF402_06865"
     CDS             1162..>1887
                     /locus_tag="VF402_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEX7800053.1"
                     /translation="MTQPIAIIGAGPAGLMAAGILSAAGRRVTVYDRMPSPARRFLMA
                     GRGGLNLTHSEVFERFVARYGKRAPHLRPALEGFTPKDLRDWADDLGAQTFVGTSGRV
                     FPRAMKASPLLRAWLARLAAQGVDVRMRSEWRGWDDRGRLRIGDEAVAADAVVLALGG
                     ASWPKLGADGGWTARLAEYGVQIAPFQPANAGFMTDWSEAMRRFAGEPLKNIALGFGG
                     RSVRGEIMLTRHGIEGGAVYALSG"
BASE COUNT          309 a          625 c          659 g          294 t
ORIGIN      
        1 atgcgggctg atccagcccg taacggaagt ggccggtgat cgcatagcgc agcaggtcgt
       61 tattctcctg cacgctcatg cccatgttct gaatgaccat caggtcgccc gcgcccagcc
      121 aggcgcgccg gtcgctgacg atggagatgt gcgggcgccc gatcggtcgc gtgaccagaa
      181 tgtcgccggg ccggtaatcc gcgccgtcgc ggctaatcgg cagaatcttg ccgaagcgcg
      241 agaagaacac cgccagattg ggcacgcggc gatggtcgat gttcgggtcc ggcgccgtca
      301 ggccccacag cttcggatag aggtcgaaat gcgccagcat accctcatgc acgcgctgtt
      361 gcaggtcgat ccccagcccg cgataggcgc gcacgatcac gtcggcgcag acgccgcgat
      421 ccggcctgac gtcgccgccc ggataggcga tcggcgtata gcgcgcgtcg taggacgtcc
      481 agccgcgcat ctgggcgcgc gccgcccgca ggaacaccgc cgtgcgcccg ccgaccgcct
      541 cgcgcgcggc ttggcatccc gtcagggcca gcgccggcaa agccgcgatc accccccgcc
      601 ggttcatgtt atttcgtcgc gcgctccagc cccttgccgg cggcggtgat gtcctgaccg
      661 acgccctgaa tagtgtggca ggcggccagc agcggcgcca gggccagaag ggacacgatg
      721 acgagacgct tcatgatgga tcatccttgg aacggttaac cgcatccgtc ttacgtccgg
      781 cgcgcgccac tgacaagacg cacgccggcg cgcgacgaaa aaaggccgtc ggcccggaga
      841 cgccgacggc ctttttcccg gatcggtcag gctcagtggc tggcggcgtc gctgacgccg
      901 tggccgacgg cgctgatatc cttgccggcg ccctgcaccg tatgacaggc cgccagcagg
      961 ggcagcagag ccatgacgga aataagaaca atgcgcttca tggtctttcc ctttcttcgc
     1021 atacatggcg gaccgacgcc cgccatcgct cacaaagtcc gtgatcggcc ataaggattc
     1081 aatgaggcgc gatgcggtaa aatataagcc cgctcatgcg tcccgctggc cagagcgcgg
     1141 ccaaagcgct atgggccgga tatgacacag cccatagcca tcatcggcgc aggcccggcg
     1201 ggcttgatgg ccgcggggat tttgagcgcc gccgggcgca gggtgacggt ttacgaccgc
     1261 atgccctcgc ctgcgcgcag gttcctgatg gccgggcgcg gcgggctgaa cctgacccat
     1321 tcggaggttt tcgagcgctt cgttgcgcgc tacggcaagc gcgcgccgca tctgcgtccg
     1381 gcgctggagg gcttcacgcc gaaggatttg cgcgactggg ccgacgatct gggcgcgcag
     1441 accttcgtcg gcacgtccgg gcgcgtgttt cccagggcga tgaaggcgtc gccgctgctg
     1501 cgcgcgtggc tggcgcggct ggcggcgcag ggcgtcgacg tgcggatgcg ttccgaatgg
     1561 cgcggctggg acgatcgggg ccgcctgcgg atcggagacg aagccgtcgc ggcggacgcg
     1621 gtcgtgctgg cgctgggcgg cgccagctgg ccgaaactcg gcgcggacgg cggctggacc
     1681 gcgcgtctgg ccgaatacgg cgttcaaatc gcgccgttcc agcccgccaa cgccggtttc
     1741 atgacggact ggagcgaggc catgcggcgc ttcgcgggcg aaccgctgaa aaacatcgcg
     1801 ctcggctttg gcggacgcag cgtcaggggc gagattatgc tgacgcgcca cggaatcgag
     1861 ggcggcgccg tttacgccct gagcggc
//
LOCUS       DASVLL010000413         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_512001,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000413 DASVLL010000000
VERSION     DASVLL010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512001"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VF402_06870"
     CDS             <1..129
                     /locus_tag="VF402_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800054.1"
                     /translation="CLVGKQMRLTPGGLTRVALTEGSLVVNSSQGGGVKDTWVLED"
     gene            131..1066
                     /locus_tag="VF402_06875"
     CDS             131..1066
                     /locus_tag="VF402_06875"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX7800055.1"
                     /translation="MLSRTAENIFWMARYIERAESTARLIQMGQRMAILPGPIHREEW
                     RSVLRVTGCDGLIDGERAVGEADIVQLLLLDEGNPSSIRSCMMRARANGRAVRTALTQ
                     DMWEALNDDWRKLESYDAASARRHLPGLLDWVKTRASTFRGAAETGQIRNEGHDFLRI
                     GGALERAEMTLRLLDVKYYVLLPETSVVGGARDHYQWTSVLHALSAARAYHHVYGGGD
                     YTPWRIADLLILNAQFPRSLSFCFGQIAWHLDRLAAAHGGRSAAQDVAAGLLSELRDR
                     ESGEVFEAGLHEFVQNALAVTNRLSREIAQAYHFA"
     gene            1075..>1500
                     /locus_tag="VF402_06880"
     CDS             1075..>1500
                     /locus_tag="VF402_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7800056.1"
                     /translation="MLLVVRHLTHMRYPHGAAGLALRLKLFPQSYDGQKVIDWHVEAD
                     GREIGPSIVGGYGERYAQWLAQGPRETLDIVAYGRVETENTAGIVKGLRRAPPPAIFL
                     RETALTRPDAAIRALGGGLGPDGGALAGLHALSRAVRDSL"
BASE COUNT          225 a          524 c          526 g          225 t
ORIGIN      
        1 tgcctggtcg gcaagcagat gcggctgacg cccggcgggc tgacccgcgt cgcgctgacc
       61 gaaggctcgc tggtcgtgaa ctcctcgcag ggcggcggcg tgaaggacac atgggtgctg
      121 gaagactgac atgctgagcc gcaccgccga aaacatcttc tggatggcgc gctatatcga
      181 gcgcgccgaa agcaccgcgc gcctgatcca gatggggcag cgcatggcga tcctgcccgg
      241 cccgatccac cgcgaggaat ggcgctcggt gctgcgcgtc acgggctgcg acgggctgat
      301 cgacggcgag cgcgcggtgg gcgaggccga tatcgtccag ttgctgctac tcgacgaggg
      361 caatccgtcg tcgatccgtt cctgcatgat gcgcgcccgg gccaatggcc gcgcggtgcg
      421 caccgcgctg acgcaggaca tgtgggaggc gctcaacgac gactggcgca agctggaatc
      481 ctacgacgcg gccagcgcgc gccgccatct gcccggcctg ctcgactggg tcaagacgcg
      541 cgcctccacc tttcgcggcg cggcggaaac cgggcagatc cgcaacgagg gccacgactt
      601 cctgcgcatc ggcggcgcgc tcgaacgcgc cgagatgacg ctgcggctgc tcgacgtgaa
      661 atactatgtg ctgctgcccg aaacctcggt ggtcggcggg gcgcgcgatc attatcaatg
      721 gacctcggtg ctgcacgccc tgtcggcggc gcgcgcctac caccacgtct atggcggcgg
      781 cgactatacg ccgtggcgca tcgccgacct gctgatcctc aacgcgcagt ttccgcgctc
      841 gctgtccttc tgcttcggcc agatcgcctg gcacctcgac cggctggccg ccgcgcacgg
      901 cgggcgcagc gcggcgcagg acgtcgccgc cggtctgctg tccgaactgc gcgaccgcga
      961 gagcggcgaa gtcttcgagg cgggcctgca cgaattcgtg cagaacgcgc ttgccgtcac
     1021 caaccgcctg tcgcgcgaga tcgcgcaggc ctaccatttc gcctgaaggt tccgatgctg
     1081 ctggtcgtcc gccatctcac gcatatgcgc tatccgcacg gggcggcggg gctggcgctg
     1141 cgcctgaaac tgtttccgca gagctatgac gggcagaagg tcatcgactg gcatgtcgag
     1201 gcggacgggc gcgagatcgg tccctccatc gtcggcggct atggcgagcg ctacgcgcag
     1261 tggctggcgc aaggtccgcg cgagacgctc gacatcgtcg cctacggccg cgtcgagacg
     1321 gaaaacaccg ccggaatcgt caagggcctg cgccgcgcgc cgccgcccgc gatcttcctg
     1381 cgcgaaacgg cgctcacccg tcccgacgcc gcgatccgcg cgctcggcgg cgggctgggg
     1441 ccggacggcg gcgcgctggc cggtctgcac gccctgagcc gggcggtccg cgactcgctg
//
LOCUS       DASVLL010000414         3223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_296344,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000414 DASVLL010000000
VERSION     DASVLL010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296344"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VF402_06885"
     CDS             complement(<1..1363)
                     /locus_tag="VF402_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX7800057.1"
                     /translation="MSSAHAEQAQPLTPRRVFSDPDLSGPKAVGAKLSPDGELLTFLR
                     PKDDDARIQDLWAAPVKGGAPYRLIDADRLASPDKTLSEAETARRERMRVSARGIVAY
                     DWSDDSQSILVPLDGDIYMIDRATGAVRRVTDTPGDEVDAKLSPDGKSVSYVRDQTLY
                     VRDLAGNIETAATPKGEGAISYGTAEFIAQEELSRYTGYWWSPTSRQIAYEKSDESGV
                     KIAERVEIGGDGVSTMAQRYPFAGTPNAVVDLYIKTLGRDAPVKVDLGRDPDIYLARV
                     TWARDGGSLYVQRLSRDQKRLDLLRVDAETGASVVVLSETADTWVEVYDDFRLLGDGG
                     FIWASERDGNKHFYLYGADGRLVHQITHGDWPVDALVDVNEQTGDLWFTACKDTPTER
                     RLYRVNYRHPGEPAAITPAGGWWTVSASGGGKAFIGTYADPRTPPQTALYDGDGRRLR
                     WIEE"
     gene            complement(1514..1756)
                     /locus_tag="VF402_06890"
     CDS             complement(1514..1756)
                     /locus_tag="VF402_06890"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX7800058.1"
                     /translation="MKDLIRTNNPALISFVESLLKEADIPYFVADQAISSAEGSIGMF
                     PKRILVPEDYQDKARRWLTEAGLEAELRPLKPNGWD"
     gene            complement(1892..>3223)
                     /locus_tag="VF402_06895"
     CDS             complement(1892..>3223)
                     /locus_tag="VF402_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800059.1"
                     /translation="ATVAFNVNGPLFKLPAGDVEAAFGVEHRYAKIDDEPDPNSVSGN
                     LLNYSTAQPTIGHTGVNEAYGEIDVPILKNLPAANELSLELAGRYTEDSKSGSADTYK
                     AGLVWKPFSWLTLRGSQGTSFRAPALFELFQGAQSGYLTFTDDPCNDLASQTHASPNR
                     VKNCALEVPADFEQKNAVEDVSVGGAENHLKPETSVNNTYGFVFRPTFLPSYLGKFDL
                     AVDYFDIQISNGIAQVGGKNLLDLCYDSAAGRAGGSYCNYSSRDPADNSLIVQDGYVN
                     VSDQGTKGYDYDLRWTQRFGDLTARIDGQATHFTSQTYRLLPTDPVTQSNGWVGSPAW
                     SGSVNAQLTYHKWSANYGMEWVGAMDSSDYEEVDRNTDPYVFWTKDYFIHTVSVEYDA
                     DSWTILAGVRNLTDQNPPQVSTYLQRIGNAPLYSGYDLIGRQFYFDLTKKF"
BASE COUNT          577 a         1026 c         1025 g          595 t
ORIGIN      
        1 tttcctcgat ccagcgcagg cgcctgccgt cgccgtcata gagcgcggtc tgcggcgggg
       61 tccgcggatc ggcgtaggtt ccgatgaacg ccttgccgcc gccggacgcc gacaccgtcc
      121 accagccgcc cgccggggtg atcgccgccg gttcgcccgg atgccggtaa ttcaccctat
      181 agaggcggcg ctcggtcggc gtgtccttgc aggccgtgaa ccagagatcg ccggtctgtt
      241 cgttgacgtc gaccagtgcg tcgaccggcc agtcgccgtg cgtgatctgg tggaccagcc
      301 tgccgtcggc gccgtagaga taaaaatgct tgttcccgtc gcgctccgac gcccagatga
      361 agccgccgtc gcccagcagc cggaaatcgt cataaacctc gacccaggta tcggcagtct
      421 cgctcagcac gaccacggac gcgcccgtct ccgcatcgac gcgcagcagg tcgagccgct
      481 tctgatcgcg gctcaggcgc tgcacgtaga ggctcccgcc gtcccgcgcc caggtaacgc
      541 gcgccagata gatgtcggga tcgcgcccca gatcgacctt gaccggcgcg tcccggccga
      601 gggtcttgat gtacaggtcc acgaccgcgt tgggcgtgcc cgcgaacgga tagcgctgcg
      661 ccatcgtgga gacgccgtcg ccgccgatct cgacgcgctc agcgatttta acgccgcttt
      721 cgtcggattt ttcgtaggcg atctgccgcg atgtcggact ccaccaataa ccggtgtagc
      781 ggctcagttc ctcctgcgcg atgaactccg ccgttccgta agagatcgcg ccctcgccct
      841 tcggcgtcgc cgccgtctcg atattgccgg ccagatcgcg cacatagagg gtctggtcgc
      901 gcacgtagga aacggattta ccgtccggcg acagcttggc gtcgacctcg tcgcccggcg
      961 tgtcggtgac gcgcctcacc gccccggtcg cgcggtcgat catgtagatg tcgccatcca
     1021 gcgggacgag aatggattgg gaatcgtcag accagtcata ggcgacgatg ccgcgcgccg
     1081 acacgcgcat gcgctcgcgc cgcgccgtct cggcctccga taaggtcttg tccggcgagg
     1141 cgagacggtc ggcgtcgatc agccggtacg gcgcgccgcc cttcacgggt gcggcccaca
     1201 gatcctgaat acgggcgtcg tcgtctttcg gcctgaggaa ggtcagcagt tcgccgtccg
     1261 gcgacagctt ggcgccgacc gccttggggc cgctcaggtc gggatcggaa aacacccgcc
     1321 gcggcgtcag gggctgggcc tgttcggcgt gggcggaaga catggcgccg gctccggtca
     1381 tcatcgttgt cgctagaaga aaggcgccga aaagggcggc ggagacgcgc ctgacatgca
     1441 tgaacagctc ccggaaacga accgcgacgc tagaacgctc accggcgggc gtcaagttgc
     1501 agatgcaacc agcctaatcc caaccgttcg gcttcaaggg gcgcagctcg gcctccagtc
     1561 ccgcctcggt cagccagcgg cgcgccttgt cctgataatc ctccggcacg aggatgcgtt
     1621 tcgggaacat gccgatcgag ccctcggcgg agctgatcgc ctgatccgcc acgaaatagg
     1681 gaatatcggc ttctttcagc aacgattcga cgaacgaaat cagggccgga ttgttggtgc
     1741 ggatcagatc tttcatgcac ggcagaatga tacgcttcgc cgcgccgggg caagaggctg
     1801 tatctggaaa agcgtcaaag cctccccaca acccataaaa aagcggcggc ggacatgtcc
     1861 gccgccgcct cttttgcact tcgttccgtt atcagaactt cttggtcagg tcgaagtaga
     1921 actgacggcc gatcaggtca tagcccgaat acagcggcgc attgccgatg cgttggagat
     1981 aggtcgagac ttgcggcggg ttctgatcgg tcaggttgcg cacgccggcc aggatggtcc
     2041 aggaatcggc gtcgtattcg accgacacgg tgtggatgaa gtagtccttc gtccagaaga
     2101 catacggatc ggtattgcga tcgacctctt catagtcgct ggaatccatc gcgccgaccc
     2161 attccatgcc gtaattggcg gaccacttgt ggtacgtcaa ctgcgcgttg accgaaccgc
     2221 tccaggccgg cgaaccgacc cagccgttcg attgggtgac cggatccgtg ggcaacagac
     2281 gataggtctg cgacgtgaag tgcgtcgcct gaccgtcgat gcgcgcggtc aggtcgccga
     2341 aacgttgcgt ccagcgcaga tcgtaatcgt aacccttcgt gccctgatcc gagacgttca
     2401 cataaccgtc ctgaacgatc agcgaattgt ccgccggatc gcggctcgaa tagttgcaat
     2461 aggagccgcc cgcacgaccc gccgcgctgt catagcagag gtcgagcaga ttcttgccgc
     2521 cgacctgggc gatgccgttg ctgatctgga tgtcgaaata atcgacggcg agatcgaact
     2581 tgcccagata gctcggcagg aaggtcggac ggaaaacaaa gccataggtg ttgttcaccg
     2641 aggtttccgg cttcaggtgg ttctcggcgc cgccgacgct gacgtcctcg acagcgttct
     2701 tctgttcgaa atcggccggc acttcgagcg cgcagttctt gacccggttc gggctggcgt
     2761 gggtctgcga ggccagatcg ttacacggat cgtcggtgaa cgtcaggtag ccgctttgcg
     2821 cgccctggaa cagttcgaac agggccggcg cccggaagga cgtgccttgc gaaccgcgca
     2881 gggtcagcca gctgaacggt ttccagacca gaccggcctt ataggtgtcc gccgaacccg
     2941 acttggaatc ctcggtgtaa cggcccgcca gttccagcga cagctcattg gccgccggca
     3001 ggttcttcag gatcggcaca tcgatttcgc cgtaggcttc attgacgccg gtgtggccga
     3061 tcgtcggctg cgccgtcgaa tagttcagca ggttgcccga aacgctgttg ggatcgggct
     3121 cgtcgtcgat cttggcgtaa cgatgctcga cgccgaaggc cgcctcgacg tcgccggccg
     3181 gcagcttgaa caggggaccg ttgacgttga aggccacggt ggc
//
LOCUS       DASVLL010000415         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_188000,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000415 DASVLL010000000
VERSION     DASVLL010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188000"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VF402_06900"
     CDS             complement(<1..210)
                     /locus_tag="VF402_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7800060.1"
                     /translation="MTRSPLSRRSFLAMTTAAAAAGLYARATEAQPPADGAVSLAGRW
                     AFALDPADEGAAAGWHTKPLPADAVI"
     gene            409..>1708
                     /locus_tag="VF402_06905"
     CDS             409..>1708
                     /locus_tag="VF402_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX7800061.1"
                     /translation="MTFFEMTDQNGAVRPCYAEVQRWVDEVGIEGLNARLKEAETIFR
                     RIGITFAVYGEGGDPERLIPFDLLPRIFSAAEWRVLDRGIRQRARALNAFLYDVYHRG
                     EIIRAGVVPGDLVYRNKAFEPEMIGFDPPGRVYSHIVGIDIIRTGDSGFQVLEDNCRT
                     PSGVSYMLENREIMQRMFPELFGASRVAPVDSYPNDLLATLKEVAPAGAGSDPTVVVL
                     TPGAFNSAYYEHSFLADLMGVELVEPMDLYVDGDRVWMKTTHGPRRVDVIYRRIDDDY
                     LDPLTFRPDSMLGVPGLFGAYRAGGVTICSAPGAGIADDKAIYLFVPDMIRFYLSEAP
                     ILQNVPTWRCALPDECAYVLEHLSELVAKEVHGSGGYGMLIGPRATRAEVEAYAARIR
                     ANPADFIAQPTLDLSTAPVLGHDAIVGRHVDFRPYCLVGKQ"
BASE COUNT          284 a          565 c          556 g          303 t
ORIGIN      
        1 gatgacggcg tccgccggca gcggcttcgt gtgccagccc gccgccgcgc cctcatcggc
       61 ggggtccagc gcgaacgccc agcgtcccgc cagcgagacg gcgccgtcgg cgggcggctg
      121 cgcctcggtc gcccgcgcgt aaagccccgc cgccgccgcc gcggtcgtca tggcgaggaa
      181 ggatcggcgg ctgagcgggg aacgggtcat cgggcgtcct ttaagcattc aagagcggcg
      241 cttacatccg ctcacgtatc gggataaatt ttcatgaatg tgcatgatta atctcattta
      301 gtcaattata gtataattaa ggctccacgc ctgaattacg cgccgacaaa acgggcttgc
      361 caaatacatt gtgcggtgca aacttgcagg tggaacgagg gagcgcggat gacctttttc
      421 gagatgaccg atcaaaacgg cgcggtgcgg ccctgttacg ccgaggtgca gcgctgggtc
      481 gacgaggtcg ggatcgaagg gttgaacgcg cggctcaaag aagccgagac gatcttccgc
      541 cgcatcggca tcaccttcgc ggtctatggc gagggcggcg atcctgagcg gctgatcccg
      601 ttcgacctgc tgccgcgcat cttctccgcc gccgaatggc gcgtgctgga tcgcggcatc
      661 cgtcagcgcg cccgcgcgct caacgccttt ctctacgacg tctatcatcg cggcgagatc
      721 atccgcgccg gcgtcgtgcc gggcgacctc gtctatcgca acaaggcgtt cgagccggag
      781 atgatcggct tcgacccgcc ggggcgcgtt tacagccaca tcgtcggcat cgacatcatc
      841 cgcaccggcg acagcggctt tcaggtgctg gaggacaatt gccgcactcc gtcgggcgtc
      901 agctacatgc tcgaaaaccg cgagatcatg cagcggatgt tccccgaact gttcggcgcc
      961 agccgggtgg cgccggtcga ttcctatccc aacgacctgc tggcgacgct gaaggaggtg
     1021 gcgcccgccg gggcgggcag cgatcccacc gtcgtggtgc tgacgccggg cgcgttcaat
     1081 tcggcctatt acgagcacag cttcctggcc gacctgatgg gcgtcgaact ggtcgagccg
     1141 atggacctct atgtcgacgg cgaccgcgtg tggatgaaga cgacgcacgg cccccggcgg
     1201 gtcgacgtca tctatcgccg catcgacgac gactatctcg atccgctgac cttccgcccg
     1261 gactccatgc tgggcgtgcc gggcctgttc ggcgcctatc gcgcgggcgg ggtgacgatc
     1321 tgctccgcgc ccggcgccgg gatcgccgac gacaaggcga tctatctgtt cgtgcccgac
     1381 atgatccgct tctatctttc cgaagcgccg atcctgcaaa acgtgccgac atggcgctgc
     1441 gcgctgcccg acgaatgtgc ctatgtgctg gagcatctca gcgaactggt cgccaaggag
     1501 gtgcatggtt cgggcggtta cggcatgctg atcggcccca gggcgacgcg cgccgaggtc
     1561 gaggcttacg ccgcgcgcat ccgcgccaat ccggccgact tcatcgcgca gccgacgctc
     1621 gacctgtcga ccgcgccggt gctgggccat gacgcgatcg tcgggcgcca cgtcgatttc
     1681 cgcccctact gcctggtcgg caagcaga
//
LOCUS       DASVLL010000416         6550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_457866,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000416 DASVLL010000000
VERSION     DASVLL010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457866"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VF402_06910"
     CDS             complement(<1..671)
                     /locus_tag="VF402_06910"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX7800062.1"
                     /translation="MALPAHPSAALPPPDLPEAPVPEAPPRAAGVLPVSEQELVVRLG
                     QMPSAIAAPLLRAHMPALTPQTLLALIMATGEAHHRLIAARKGLDWRVAKALLRTGRD
                     GVILILLDNGGVDFDAEDEAFLAARALESPDIRAAVLAHPRLKTAHARLNAGEPLSQA
                     NLRLLARLRAGSSGGFTREAAKRLDLDADALWRRLHAGSVTPLALLACALGFDRAAFG
                     RILGH"
     gene            877..2235
                     /locus_tag="VF402_06915"
     CDS             877..2235
                     /locus_tag="VF402_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800063.1"
                     /translation="MSDMDPDTDSGRRTTAGVFTPSPGRERLEIAHDRAVEIVERLRE
                     THVDITGHAFLLRLDTLIVRLGARWEIKRELVFEHLKTAFERLYQEPNWCIQVHEDTW
                     MGMLPEIGSRKGALVMTQIWRDLCGFFVGDMSALEVPLYEVLVEDVDCFSLSRIDLRT
                     WFDAAEVDDAPVKARGGAFHAEEGRGPSDAPSSTPIQRPLIVAASVTYAGRSLKVASS
                     AEPLFEMKKMVMIGHRLEALVMDEVDNTLLDRKALANLDWGLREMADIVNIDQGLKLL
                     KMKAPEQRKMVMLVPAAFSTFASARARQRITSEVSRAAVEMGLKVLFEVRGLDGVPPH
                     RILEIVSMIKPFCMTVVGSVSADRKAIAALSKCGLSGVSLEYDGVRRDEEALRDYLSM
                     LSQTAKTSAGACMIQGFDNYRQMAVARLAGVTHASVKTAALMSPRGHAEAHEAEPEAR
                     EA"
     gene            2362..2859
                     /locus_tag="VF402_06920"
     CDS             2362..2859
                     /locus_tag="VF402_06920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7800064.1"
                     /translation="MQIMPEKSADEELIRAVTTAAFLEAEHSDGNEASIVDELRDTDA
                     LTISLVAADGGNIVGHVAFSPVLIDGFHDGWFGLGPVSVIPNCQRKGIGTALIEAGLA
                     DLRRQGSRGCVVLGDPAYYRRFGFAVDPALYLAGVPPEYFQSLSFDGQPRSGRVAYHP
                     AFGGG"
     gene            complement(2905..3765)
                     /locus_tag="VF402_06925"
     CDS             complement(2905..3765)
                     /locus_tag="VF402_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX7800065.1"
                     /translation="MRWQDGRESGVEDRRGGGVGAPHIIGGGGIGVVVVVLLLSFVFH
                     VDPRQLLSATDADTTQTASGPAAVSACPQGDETCRFVDVVHTSTDDVWSAVFRSEGKA
                     YRPSTIVLYSQGAQTGCGLGQSAMGPFYCPADEKIYLDLDFFNTLEQQLGARGDFARA
                     YVIAHEEGHHIQKLLGVSDAVHDKEQHLSEKAANALSVRLELQADCYAGVWARRADDR
                     MHWLEAGDIDEALGAAAAVGDDTLQKETQGRVVPDSFTHGTSAQREHWFKAGFAGGDP
                     ADCDTFSGEI"
     gene            4046..5113
                     /locus_tag="VF402_06930"
     CDS             4046..5113
                     /locus_tag="VF402_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7800066.1"
                     /translation="MRLKPVWLTAGVMLAVMAGAQGCAKKPKADASAQHAATVNYAVV
                     QTGVAPVRITGSGSVSAWQEVPVGAETGGLTAVALLADEGQEVKQGQPLLKMNDVLLQ
                     AQLKQASANYDQANKAYQRAETLYKQGYLSAAGLDQAQASMLTTQAALDTAQTQLSLA
                     TVRAPVSGVVSSRTAVLGQIVQQGAELFRIVRDGRIELNMEVVESDLHRIQPGMSATV
                     TSESTGPVAGTVRIVTPEVNPTTRLGYARISAPWSSGLRPGMFAQGDIDAGTQTVMEL
                     PQSAIVYSQNLPAVYLIGPDNKAHIRKVALGEHLGQAVVIKDGVAPGDRVVTTGAGFL
                     NDGDLVHPVAASGGEAARTGG"
     gene            5118..>6550
                     /locus_tag="VF402_06935"
     CDS             5118..>6550
                     /locus_tag="VF402_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7800067.1"
                     /translation="MSNGSAQRGGFQISSWSIRNPIPTIVFFLVMTIAGVMGFQSLRV
                     NNWPDIDFPIVVVTVTRAGAAPEELQNQVTRIVEDSVSGLGGVKHIQSYVNEGASTTV
                     ITFQLDEDLERATNDVRNAVAGVRSSLPGDVQDPIVSRVDNAQGQPLLTYVIRSDNMS
                     PEDLSWYVDNDVAKKALAVKGVSSLTRNGGVDREIRVELDPARLAAKGVTASDISSQL
                     ASYNVNMPGGRFNSGTSEQTIRTVGGADTVTALSESRINLSNGQSVRLGDLGTVTDTW
                     SEPRTRARYNGNEVVGFSVERTRGTSEKRVAEGVRAAVKALAASNPHVQIEEVASSVD
                     EVNRSFDSSMEALVIGALLAVAVVFLFLRDWRATLVSAVAMPMSLLPTFYVMSVLNQS
                     FNIVTLLALSLTIGILVDDAIVEIENIVRHMREGKKPYPAAIEAADEIGLAVVATTAT
                     LIAVFAPTGFMPGIVGQFFKSFAIASCV"
BASE COUNT         1099 a         2105 c         2264 g         1082 t
ORIGIN      
        1 caatggccga ggatgcgccc gaaggcggcg cggtcgaaac cgagcgcgca ggccagcagg
       61 gccagcggcg tgaccgatcc ggcgtgcagc cgccgccaca gcgcgtccgc gtcgagatcg
      121 aggcgtttgg cggcttcgcg cgtgaacccg ccgctcgatc cggcgcgcag ccgcgccagc
      181 aggcgcaggt tggcctgcga aagcggctcg cccgcattca gccgcgcatg cgcggttttc
      241 aggcgcggat gggccaggac ggcggcgcgg atgtccgggc tttccagcgc gcgcgcggcc
      301 aaaaacgcct cgtcctcggc gtcgaaatcg acgccgccat tgtcgagcag gatcaggatc
      361 acgccgtcgc gcccggtgcg cagcagcgcc ttggccacgc gccagtccag ccccttgcgc
      421 gccgcgatca ggcggtgatg cgcctcgccc gtggccatga tcagcgccag cagggtctgc
      481 ggcgtcaggg cgggcatatg ggcgcgcagc agcggcgcgg cgatggcgga gggcatctgg
      541 cccagccgca cgaccagttc ctgctcggac acgggcagga cgcccgcggc gcgcggcggc
      601 gcttccggaa cgggcgcttc cggcaggtcg gggggcggga gagcggcgga cgggtgagcg
      661 ggcagggcca aggcggcgtt tatccctggt gacgaatcag gcgaaacgaa tcgagtgtag
      721 caaatgtggc ggcggcaggg tgaacacttg cttaataagg cgttcgccgc ctttttcgac
      781 gcggaggaga aaaatctcgg ggcgtgttaa tcttgtctca atcgaccctt tctaaggtcg
      841 tgtccgagtg ttttgagtat cttcggaagg gccgacatgt ccgacatgga ccccgacacg
      901 gactccggcc gccggacaac ggcgggcgtc tttacgccgt ctcccggacg tgagcggctg
      961 gagatcgcgc acgaccgcgc ggtggagatc gtcgagcggc tgcgcgaaac ccatgtcgac
     1021 atcaccggcc acgcctttct tctgcgcctc gacacgctga tcgtgcgcct gggcgcgcgc
     1081 tgggagatca agcgcgagct ggtgttcgag catctgaaaa cggcgttcga gcgcctgtat
     1141 caggagccga actggtgcat ccaggtgcat gaagacacgt ggatgggcat gctgcctgag
     1201 atcggctcgc gcaagggcgc gctggtcatg acgcagatct ggcgcgacct gtgcggcttc
     1261 ttcgtcggcg acatgtcggc gctggaggtg ccgctgtacg aggtgctggt cgaggacgtc
     1321 gactgcttct cgctcagccg catcgatctt cggacctggt tcgacgccgc cgaggtggac
     1381 gacgcgccgg tcaaggcgcg cggcggcgcc ttccacgcgg aggaggggcg cggcccgtcc
     1441 gacgcgccgt cctcgacccc gatccagcgc ccgctgatcg tcgccgcctc ggtgacctat
     1501 gcggggcgct cgctgaaagt ggccagctcc gccgaacccc tgttcgagat gaagaagatg
     1561 gtcatgatcg gccaccgtct cgaagcgctg gtcatggacg aggtcgacaa cacgctgctg
     1621 gaccgcaagg cgctggccaa tctcgactgg ggcctgcgcg agatggccga catcgtcaac
     1681 atcgatcagg ggctgaagct gttgaagatg aaggcgccgg aacagcgcaa gatggtcatg
     1741 ctggtcccgg cggcgttctc gaccttcgcc tcggcgcggg cgcggcagag gatcacgtcc
     1801 gaagtatcgc gcgcggcggt ggagatgggg ctgaaggtgc tgttcgaggt gcgcgggctg
     1861 gacggcgtgc cgccgcaccg catcctcgaa atcgtgtcga tgatcaagcc gttctgcatg
     1921 acggtggtcg gctcggtcag cgccgaccgc aaggcgatcg cggcgctgag caagtgcggc
     1981 ctgtcgggcg tcagcctcga atatgacggc gtccggcgcg acgaggaggc gttgcgcgac
     2041 tatctgtcca tgctcagcca gaccgccaag acctcggcgg gcgcctgcat gatccagggc
     2101 ttcgacaatt accgccagat ggcggtggcg cggctggccg gcgtgaccca cgccagcgtc
     2161 aagaccgccg ccctgatgtc cccgcgcggc cacgccgaag cgcacgaagc cgaaccggaa
     2221 gcgcgcgaag cctgaggcgg ggcttttcaa gagaaggtcc cctttttttg ccgtgcttgt
     2281 tggggagaag cggcgcgatg acgcgccgct cgaacaccac ttcgccattg agaggctacg
     2341 acgccgtggt agaataagct gatgcagata atgcccgaga aatctgcgga tgaagagctt
     2401 atccgcgccg tgaccactgc agcgttcctc gaagcagaac acagtgatgg taacgaagcg
     2461 tcgatcgttg atgagttgcg ggatacagat gctttgacga tctcactcgt cgcggcggat
     2521 ggtgggaaca tcgtaggcca cgtggccttc tccccagtgt tgatcgatgg ctttcacgac
     2581 ggctggttcg ggcttggtcc cgtgtcggta atcccaaatt gtcagcgaaa gggcattgga
     2641 accgcgctta tcgaggcggg tctcgccgac ctaagacgcc agggctccag aggttgcgtg
     2701 gttcttggcg atcccgcgta ctaccgtcgc ttcggctttg cggtcgatcc cgccctttat
     2761 ttagcgggag tcccgccaga gtattttcaa agcctctcgt tcgacgggca gcctcgaagc
     2821 ggaagggtcg cttatcaccc cgcttttggc ggcggctgac gagcccattc tcggcttcgc
     2881 taaccaggat aatgggtttg cggcctaaat ctccccgctg aacgtgtcgc aatcggcggg
     2941 atcgccgccg gcgaatccgg ccttgaacca gtgctcgcgc tgcgccgacg ttccgtgcgt
     3001 gaagctgtcg gggacgacgc ggccttgcgt ctccttttgc agcgtgtcgt cgccgaccgc
     3061 cgccgcggcg cccagcgctt cgtcgatgtc gccggcctcc agccagtgca tccggtcgtc
     3121 ggcgcgcctg gcccagaccc cggcgtagca gtcggcctgc aactccagcc gcaccgacag
     3181 ggcgttggcg gccttttcgc tgagatgctg ttccttgtca tgcacggcgt cggacacgcc
     3241 gagcagcttc tggatatggt ggccttcctc gtgcgcgatc acataggcgc gcgcgaagtc
     3301 gccgcgtgcg ccgagctgct gctccagcgt gttgaagaag tcgaggtcga gatagatctt
     3361 ctcgtcggcc gggcaataga acggccccat cgccgactgg ccgaggccgc agccggtctg
     3421 cgcgccctgc gaatacagca cgatggtcga ggggcggtac gccttgccct cggagcggaa
     3481 gaccgccgac cagacgtcgt cggtcgaggt gtggacgacg tcgacgaagc ggcacgtctc
     3541 gtcgccctgc gggcaggccg acaccgccgc cgggccgctg gcggtctgcg tggtgtccgc
     3601 gtcggtggcc gacagaagct ggcgcggatc gacatgaaag acgaaggaca gcagcagcac
     3661 gacgacgacc acgccgatcc cgccgccgcc gatgatgtgc ggcgcgccga cgccgccgcc
     3721 gcgccggtcc tcgaccccgc tttcacgtcc atcctgccag cgcatgtcgg gagcctccgt
     3781 ccttcaggtt tcccgactat cggtcaggcc ggggcgcatt tcaagcccgc ggccggggcg
     3841 attgctgaca gtttccgtcg cgcgcgggcc gcattcgtgg caaaagaaaa cgcagccttt
     3901 cgccgcgcgc caagatgtag gatttttaat gttaggtttc gttgactcgc cgcgaggccg
     3961 acagcatcat gcgcgcaaaa aaccgggcct gaattcaggg ccaaattcag gaatcgacat
     4021 caaagtgcag aattcggggc agaagatgag gctgaagccg gtctggctga cggcgggcgt
     4081 tatgctggcc gtcatggcgg gcgcgcaggg ctgcgcgaag aaacccaagg ccgatgcgtc
     4141 ggcgcaacac gccgcgaccg tgaattacgc cgtcgtgcag accggcgtcg cgccggtgcg
     4201 catcaccggc tccggctcgg tcagcgcctg gcaggaggtg ccggtcggcg ccgagacggg
     4261 cgggctgacc gccgtggcgc tgctggccga cgaggggcag gaggtcaagc agggccagcc
     4321 gctgctgaag atgaacgacg tgctgctgca ggcgcagctc aagcaggcca gcgccaatta
     4381 cgatcaggcc aacaaggctt atcagcgcgc cgaaacgctt tataaacaag gctatctgtc
     4441 cgccgccggg ctggatcagg cgcaggcctc catgctgacg acgcaggccg cgctggacac
     4501 ggcgcagacg caattgtcgc tggcgacggt gcgcgcgccg gtgtcgggcg tggtctcgtc
     4561 gcgcacggcg gtgctgggcc agatcgtgca gcagggcgcc gaactgttcc gcatcgtgcg
     4621 cgacggccgc atcgagctga acatggaggt ggtcgaatcc gacctgcacc gcattcagcc
     4681 gggcatgagc gcgaccgtga ccagcgaatc gaccggaccg gtcgccggaa cggtgcgcat
     4741 cgtcacgccg gaggtcaatc ccacgacgcg gctcggctat gcgcgcatct cggcgccgtg
     4801 gtcgtcgggc ctgcgtccgg gcatgttcgc gcaaggcgat atcgacgccg ggacgcagac
     4861 cgtgatggag ctgccgcaat cggccatcgt ctattcgcag aacctgccgg cggtctatct
     4921 gatcggaccg gacaacaagg cgcatatccg caaggtggcc ttgggcgaac acctcggtca
     4981 ggccgtggtg atcaaggacg gcgtcgcgcc gggcgaccgc gtggtgacca ccggcgccgg
     5041 cttcctgaac gacggcgatc tcgtgcatcc ggtcgccgcg tcgggcggcg aagcggccag
     5101 gaccgggggc taggcgcatg tcgaacggct cagcgcagcg cggcggcttc cagatatcgt
     5161 cctggtcgat ccgcaatccg attccgacca tcgtcttctt cctcgtgatg accatagcgg
     5221 gcgtcatggg ctttcagtcg ttgcgggtga acaactggcc cgacatcgat tttcccatcg
     5281 tggtcgtgac ggtgacgcgc gcgggcgcgg cgcctgagga gttgcagaac caggtcacgc
     5341 gcatcgtcga ggactcggtg tccggcctcg gcggcgtcaa gcacatccag tcctacgtca
     5401 acgaaggcgc gtccaccacg gtcatcacct tccaactgga cgaggatctg gagcgcgcca
     5461 ccaacgacgt gcgcaacgcc gtggccgggg tcaggtccag cctgcccggc gacgtgcagg
     5521 atccgatcgt gtcgcgcgtc gacaacgcgc agggccagcc gctgctgacc tatgtgatcc
     5581 gctccgacaa catgtcgccg gaggatctga gctggtacgt cgacaacgac gtggccaaga
     5641 aggcgctggc ggtcaagggc gtgtcgtcgc tgacgcgcaa cggcggcgtg gaccgtgaaa
     5701 tccgcgtcga gctggatccg gcgcgcctcg ccgccaaggg cgtgacggcc agcgatatct
     5761 ccagccagct cgcctcctac aacgtcaaca tgccgggcgg gcgcttcaat tccggcacgt
     5821 cggaacagac catccgcacc gtcggcggcg ccgacacggt gaccgccctg tcggaatcgc
     5881 gcatcaacct gtcgaacggc cagagcgtgc ggctgggcga cctcggcacg gtcaccgaca
     5941 cctggtccga gccgcgcacg cgggcgcgct ataacggcaa cgaagtcgtc ggcttctcgg
     6001 tcgagcgcac gcgcggcacc tcggaaaagc gcgtcgccga aggcgtgcgc gcggcggtca
     6061 aggcgctggc ggcgtcgaac ccgcacgtcc agatcgagga ggtcgcctcg tcggtcgacg
     6121 aggtcaaccg cagcttcgat tcgtcgatgg aggcgctggt gatcggcgcg ctgctggccg
     6181 tggcggtggt gttcctgttc ctgcgcgact ggcgcgcgac gctggtgtcg gcggtggcca
     6241 tgccgatgtc gcttctgccg accttctacg tgatgagcgt gctgaaccag tcgttcaaca
     6301 tcgtgaccct gctggccctg tcgctgacca tcggcattct ggtcgacgac gccatcgtcg
     6361 agatcgagaa catcgtgcgc cacatgcgcg aagggaaaaa accgtatccc gccgccatcg
     6421 aggccgccga cgaaatcggc ctggccgtgg tggcgacgac ggcgaccctg atcgcggtgt
     6481 tcgcgccgac cggcttcatg cccggcatcg tcggccagtt cttcaagagc ttcgcgatcg
     6541 cctcgtgcgt
//
LOCUS       DASVLL010000417         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_81405, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000417 DASVLL010000000
VERSION     DASVLL010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81405"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="moaA"
                     /locus_tag="VF402_06940"
     CDS             complement(<1..890)
                     /gene="moaA"
                     /locus_tag="VF402_06940"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX7800068.1"
                     /translation="MSASRPLIDGFGRRVSYVRLSVTDRCDLRCAYCMSAHPTFLPRG
                     DLLSFDELERLCGFFVAHGVERLRVTGGEPLVRRGILDFLTRVGEHVHAGRLRELTLT
                     TNGTQLERFAEGLAAAGIRRVNVSCDTLRPDVFRRVTRGGDLARVLAGIEAAQGAGLQ
                     VKLNVVALAQDNRAEIPDIVAFAHARGMAVSLIETMPLGDEVEGREDQFLSLADVLAE
                     LRSFWTVEAAAEATGGPARYYRVAETGGKLGLITPLSHNFCDTCNRVRVTCAGRLYMC
                     LGQDDHVDLRAPLRDGSGEAG"
     gene            complement(937..1965)
                     /locus_tag="VF402_06945"
     CDS             complement(937..1965)
                     /locus_tag="VF402_06945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7800069.1"
                     /translation="MRILNIMLAQVRGGVETMSLRYHEALKASGVAVLSMGHPDGVLG
                     EALPSTEFRPVHALFNHDPFAAARIAAAARAFRPDLVLTHGNRATGICLLPFVGTAKR
                     TVQVIHNFRHKGQSGRVRAAICVSASVAASVRRKHPGLDVYELPNFGPLSVRAVKPAP
                     RSAVILGTLGRLHVNKGIDVMLRALAILRERGLDARLRIGGDGPLRAELEALTRELGL
                     AAAVDFVGWVADAADFLHGLDLFVLPSRVEPFGLVVAESMAAGAPIVATGIDGPKEIL
                     QDGALGTLCAPDDPAALAAAVAAAAADWPSSLDKARAAQAYAQAHFSLEAGRARLTAT
                     LDAIMAGG"
BASE COUNT          332 a          766 c          713 g          275 t
ORIGIN      
        1 cccgcctcgc cggagccgtc gcgcagcggc gcgcgcagat cgacgtgatc gtcctgcccc
       61 agacacatgt agagccgccc ggcgcaggtc acgcgcaccc ggttgcaggt atcgcagaaa
      121 ttatggctga gcggcgtgat caggcccagt tttccgcccg tctcggccac gcggtaatag
      181 cgcgccgggc cgccggtggc ctccgcggcc gcctcgacgg tccagaagct gcgcagctcg
      241 gccagcacgt cggccaggga caggaactga tcctcgcgcc cctcgacctc gtcgcccagc
      301 ggcatggtct cgatcaggct gaccgccatg ccgcgcgcat gggcgaaggc gacgatgtcg
      361 gggatttcgg cgcggttgtc ctgcgccagc gcgacgacgt tcagcttgac ctgcaggccc
      421 gcgccctgcg ccgcctcgat cccggccagc acgcgcgcca gatcgccgcc gcgcgtcacc
      481 cgccggaaca cgtcgggcct gagcgtgtcg cacgagacgt tgacgcgcct tatgcccgcc
      541 gccgccagcc cttcggcgaa gcgctcgagc tgcgtgccgt tggtggtcag ggtcagttcg
      601 cgcaggcgcc ccgcatggac gtgttcgccg acgcgcgtga gaaaatcgag gattccccgc
      661 cgcaccagcg gctcgccgcc ggtgacgcgc agccgctcga ctccgtgcgc gacgaaaaag
      721 ccgcacaatc gctccagctc gtcgaagctc agaaggtcgc cgcgcggcaa aaaggtcgga
      781 tgcgcgctca tgcagtaggc gcagcgcagg tcgcagcggt cggtcaccga cagccgcaca
      841 tagctgacgc ggcggccgaa accgtcgatg aggggacgag acgcggacat gacgcgactt
      901 taggcgcgcg cgcgcggcgg cgcaagcgtt cgcggctcag ccgcccgcca tgatggcgtc
      961 cagcgtcgcc gtcaggcgcg cacgcccggc ttcgaggctg aaatgcgcct gcgcataggc
     1021 ttgcgccgcg cgcgccttat ccaggctgga cggccagtcc gccgccgccg ccgcgaccgc
     1081 cgccgccagc gcggcgggat cgtccggcgc gcacagcgtg cccagcgcgc cgtcctgcaa
     1141 gatctccttc ggcccgtcga tgccggtcgc gacgatcggc gcgcccgccg ccatgctttc
     1201 ggccaccacc agcccgaacg gctcgacgcg cgacggcagc acgaacaggt ccagcccgtg
     1261 caggaagtcg gcggcgtcgg cgacccaacc gacgaagtcg accgccgccg caagccccag
     1321 ctcgcgcgtc agcgcctcca gctcggcgcg caacggcccg tcgccgccga tgcgcaaacg
     1381 cgcgtcgagg ccgcgttcgc gcaggatcgc cagcgcgcgc agcatcacgt cgatcccctt
     1441 gttgacatgc agccgcccca gcgtgcccag aatcacggcg ctccggggcg cgggcttgac
     1501 ggcgcgcacg ctcagcggcc cgaaattggg cagttcgtac acgtcgaggc ccggatgttt
     1561 gcgccgcacg ctggccgcca ccgaggccga gacgcagatg gcggcgcgca cccgcccgct
     1621 ctggccctta tggcggaaat tgtggatcac ctgcacggtg cgcttcgccg tgccgacgaa
     1681 cggcagaagg cagatgccgg tggcgcggtt gccgtgggtc agcacgaggt cgggccggaa
     1741 ggcgcgcgcg gcggcggcga tgcgcgcggc ggcgaacgga tcgtggttga acagggcgtg
     1801 gaccggcctg aactccgttg acggcagggc ctcgcccagc acgccgtcgg gatggcccat
     1861 gctcagcacc gcgacgccgg acgccttcag cgcctcgtga tagcgcagcg acatggtttc
     1921 gacgccgccg cgcacctgcg ccagcatgat gttgagaatc cgcatgactc gtcccgcctg
     1981 aagccgccgg agccggaaac gcgcccggaa ccgcatcaga aatacgctct aggtcgtttt
     2041 cgacatattt aaaacacctg gcgcggaaaa tcggcggaag cgtatc
//
LOCUS       DASVLL010000418         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_402527,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000418 DASVLL010000000
VERSION     DASVLL010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402527"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="VF402_06950"
     CDS             complement(<1..1249)
                     /locus_tag="VF402_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7800070.1"
                     /translation="MAKGNAPQGGRGRGGRTPVQALLYWGTVLAIWAAIFTVAFLGFM
                     AIGLPDTASLDKINKQPSITYLDRSGALIAVRGSQAAAAVDIDKLPDYVPLAFVAIED
                     RRFFTHPGFDPIGMTRGLFYDIFRRKKGGNLAGGSTITQQLARNLFLSADQNLKRKVQ
                     ELMIAIWLEHKYTKKQILALYLNRVYFGAGAYGIEAASQRYFNKPAKDLSIGEAAMLA
                     GLMKAPNSYSPLSDQERAGKRALIVLNEMVEAKVITPEQRDEALRTPINVSKTLASAH
                     AQYFVDWLDSQVRELVGPNNTEDLIVETTLDLPIQTDAERAVQTIMARDARRGVEQAA
                     LVSVDGEGRIRAMIGGVSYADSQYNRATMAKRQTGSSFKPFVYLAAMEAGYQPSTPVV
                     DEPYTLGNWSPQNYEKTYMGLIDL"
     gene            1420..>2736
                     /locus_tag="VF402_06955"
     CDS             1420..>2736
                     /locus_tag="VF402_06955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX7800071.1"
                     /translation="MSDALARRPDPVRRPDLDAAETVLRDVFGYDGFRGRQADVLSAV
                     LAGRDVLAVLPTGGGKSLCYQIPALLHAGLVLVISPLIALMSDQVAALKSRGVRAERF
                     ESSQTLDERTRIWDEARAGTLDLLYLSPEALMQGFVLERMARLKPRLIAIDEAHCISQ
                     WGHDFRPEYRALGQVRQLLPGVPMLALTATADAQTRADIKDALGVPDAEEIVAGFDRP
                     NLSLRFERRNGPAAKAVAGLLQRRKGAAGIVYCGSRDGSEKLAEALRREGHAAAAYHA
                     GLPAKTREQRLADFMSGGIGVMTATIAFGMGINKTDVRFVVHADPPSSMEAYWQEVGR
                     AGRDGAPASGVCFYGPGDLGWALRRLSLREQEGAPDQGAQKQKARDFFAFALGTDCRR
                     QGVKRYFGEDAGDPCGTCDNCLTRAAPADVTEAAQMLMSALYRLNGP"
BASE COUNT          424 a          914 c          940 g          458 t
ORIGIN      
        1 gcaggtcgat caggcccata taggtctttt cgtaattctg cggcgaccag ttgccgaggg
       61 tgtagggttc gtccaccacc ggcgtcgagg gctgataccc ggcctccatc gccgccagat
      121 agacgaacgg cttgaacgaa gacccggtct ggcgcttggc catcgtcgcg cggttatatt
      181 gcgagtcggc gtaggagacg ccgccgatca tggcgcggat gcgcccttcg ccgtcgacgg
      241 acaccagggc ggcctgctcg acgccgcgcc ttgcgtcgcg cgccatgatg gtctgcaccg
      301 cgcgctcggc gtcggtctgg atcggcaggt cgagcgtggt ctcgacgatc aggtcttcgg
      361 tgttgttcgg cccgaccagt tcgcgcacct gcgaatcgag ccagtcgacg aaatactgcg
      421 cgtgcgccga ggccagcgtc ttggagacgt tgatcggggt gcgcagcgcc tcgtcgcgct
      481 gctccggcgt gatcaccttg gcctcgacca tctcgttcag cacgatcagc gcgcgcttgc
      541 ccgcccgttc ctgatccgac agcggcgaat aggagttggg cgccttcatc agcccggcca
      601 gcatggcggc ctcgccgatg ctcagatcct tggcgggctt gttgaaatag cgctgcgacg
      661 ccgcctcgat gccataggcg ccggcgccga aatagacccg gttcaggtac agggccagaa
      721 tctgcttctt ggtgtacttg tgctccagcc agatggcgat catcagctcc tgcaccttgc
      781 gcttgaggtt ctggtccgcc gacaggaaca ggttgcgcgc cagctgctgg gtgatggtcg
      841 agccgcccgc cagattgccg cccttcttgc gccggaagat gtcgtagaac aggccgcgcg
      901 tcatgccgat cgggtcgaag cccggatggg tgaagaagcg ccggtcctcg atggcgacga
      961 aggccagcgg cacatagtcg ggcagcttgt cgatatcgac ggcggcggcg gcctgcgagc
     1021 cgcgcaccgc gatcagcgcg ccggagcggt ccagataggt gatcgagggc tgcttgttga
     1081 tcttgtccag cgaggcggtg tcgggcaggc cgatggccat gaagcccaga aacgcgacgg
     1141 tgaagatcgc cgcccagatc gccagcaccg tgccccagta caacagggcc tgtaccggcg
     1201 tgcggccgcc gcggccccgg cccccctgcg gagcgttacc cttagccatt cgtcatcctt
     1261 acctgcggac ccgttgcaac cggtccttcg tcgcgcctcg acacgccctc tataaccgat
     1321 gacgcggcgt ctgtcgcgtt aaacataaga cacgtgtaaa gccccggttt ggccaaatta
     1381 agggttaaca gcgcgcgcga accagcttac cggcgaacca tgtccgacgc cctggccaga
     1441 cgccccgatc ctgtccgccg ccccgatctc gacgccgccg aaacggtgtt gcgcgacgtg
     1501 ttcggctatg acggctttcg cggacgtcag gccgacgtgc tgagcgccgt tctggccggg
     1561 cgcgacgttc tggccgtgct gccgaccggc ggcggcaaga gtttgtgcta tcagattccc
     1621 gccctgctgc atgccggtct ggtgctggtg atctcgccgc tgatcgcgct gatgagcgat
     1681 caggtggcgg cgctgaaaag ccgcggcgtg cgcgccgagc gcttcgaatc ctcgcagacg
     1741 ctggacgagc gcacgcgcat ctgggacgag gcgagggcgg ggacgctgga ccttctgtac
     1801 ctgtcgcccg aagcgctgat gcagggcttc gtgctggagc gcatggcgcg gctgaaaccg
     1861 cgcctgatcg ccatcgacga ggcgcactgc atcagccagt ggggccacga cttccgcccg
     1921 gaataccgcg cgctggggca ggtccggcag ctcctgcccg gcgtgccgat gctggccctg
     1981 accgccaccg ccgacgccca gacccgcgcc gacatcaagg acgcgcttgg cgttcccgac
     2041 gccgaggaga tcgtcgcggg cttcgaccgc ccgaacctgt ccttgcgctt cgagcgccgc
     2101 aacggaccgg cggccaaggc cgtggcgggg ctgctgcaac ggcgcaaggg cgcggcgggc
     2161 atcgtctatt gcggctcgcg cgacggctcg gaaaagctgg ccgaggcgtt gcgccgcgaa
     2221 ggccatgcgg cggcggccta tcacgccggg ctgcccgcga agacgcgcga acagcgcctg
     2281 gccgatttca tgagcggcgg gatcggcgtg atgaccgcca ccatcgcctt cggcatgggg
     2341 atcaacaaga ccgatgtgcg cttcgtcgtt cacgccgatc cgccgtcctc gatggaggcc
     2401 tactggcagg aggtcggacg cgccgggcgc gacggggcgc cggcgtcggg cgtgtgcttt
     2461 tacggccccg gcgatctcgg ctgggcgctg cggcggctct ctttacgcga gcaggagggc
     2521 gcgccggatc agggcgcgca gaagcagaag gcgcgcgact tcttcgcctt cgcgctgggg
     2581 accgattgcc gccgccaggg cgtaaagcgc tatttcggcg aggacgcggg cgatccgtgc
     2641 ggaacctgcg acaactgcct gacgcgcgcc gcgcccgccg acgtcaccga ggcggcgcag
     2701 atgctgatgt cggccctcta tcgcctcaac ggaccg
//
LOCUS       DASVLL010000419         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_27015, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000419 DASVLL010000000
VERSION     DASVLL010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27015"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..888)
                     /locus_tag="VF402_06960"
     CDS             complement(103..888)
                     /locus_tag="VF402_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7800072.1"
                     /translation="MDEPMDESLRVAAHGALDTSPLDTAFKNLTPQGRLHGALAHKLG
                     VDILSGVYPPGRILLNEVESSSALDISRSAYREAIRILAAKGMVESRPKTGTRVTEKT
                     RWNILDPEVLRWMFETEPTESFILDLFELRLITEPAAAALAAQRRTDEQLRVMDEALT
                     VMAAETLATEAGRQADLDFHDTLMQATGNEALASLSSSISAAVAWSTRYKQRLKALER
                     DPVPDHRAVYEAIARADASDARWCMESLVRLALHDTQASMKVK"
     gene            complement(1236..2378)
                     /locus_tag="VF402_06965"
     CDS             complement(1236..2378)
                     /locus_tag="VF402_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HEX7800073.1"
                     /translation="MTRTPSRPALAAACLAAAAILSACGGGGGGGGGSSSTGSSSSSS
                     ASSSSSSSSSSSSSSSSSSSSSAGVLDSCKLSGDVSPVHDPSIIQAGGKWYVYVTDAS
                     ATQGGFIPVRCSSDKLAWSDCGFVFTTLPAWISGAVPGATTIWAPDVSYFNGLYHLYY
                     AVSTFGSNVSAIGMATNTTLDPTDPAYKWVDKGQVLTSQASSNFNAIDPNILVDAGGK
                     VWLSYGSFWEGIFQQEIDPVTGLIKTGGTAYHLARRATSVTYDPVEGASIVYKGGYYY
                     LFVSWDFCCESSPANSNYKIVVGRSTSPNGPFLDQGGADMAAGGGTILLQGNSAWSGP
                     GGQTAYVDGSGDGVIAFHALKLAQNGLDYLFVDSLDFSSGWPVIQP"
     gene            complement(2375..>2668)
                     /locus_tag="VF402_06970"
     CDS             complement(2375..>2668)
                     /locus_tag="VF402_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800074.1"
                     /translation="PCPAARFLKGALLRQKSCIILALGRQARNGACRIFRARALYVAE
                     IKDFPLYFQTLKLLRRLARAGCAEIFPLGKKLCLSILRVQRTDKSAVIQGDFA"
BASE COUNT          506 a          845 c          859 g          458 t
ORIGIN      
        1 cccggcgccg cgccgcggac gcgcgggcgc ggcggcaggc gcggcggcag gcgcggagcc
       61 aacgccggaa cggacatcac gcccggaaat cgcgagaggc ggctatttca ccttcatgct
      121 ggcctgcgtg tcgtgcaggg ccagccgcac cagcgattcc atgcaccagc gcgcgtcgga
      181 ggcgtcggcg cgggcgatgg cctcatagac cgcgcgatgg tcagggaccg gatcgcgctc
      241 cagcgccttg agccgctgct tgtagcgcgt ggaccaggcg acggcggcgc tgatcgagga
      301 gctgagcgag gccagcgcct cgttgccggt ggcctgcatc agcgtgtcat ggaagtcgag
      361 atcggcctgc cgcccggcct cggtggccag ggtttccgcc gccatgaccg tcagcgcctc
      421 gtccatgacg cgcaactgct cgtcggtgcg gcgttgcgcc gccagcgccg ccgccgccgg
      481 ttcggtgatc aggcgcagct cgaacaggtc gaggatgaac gattccgtcg gctcggtctc
      541 gaacatccag cgcagcactt cgggatcgag aatgttccag cgcgttttct cggtcacgcg
      601 cgtgcccgtc ttggggcggc tttcgaccat gcccttggcg gccaggatgc ggatcgcctc
      661 acggtaggcc gagcgcgaaa tgtcgagcgc gctgctcgat tcgacctcgt tgagcagaat
      721 ccggcccggc gggtaaacgc cgctgaggat gtccacgccc agcttgtgcg ccagcgcgcc
      781 gtgcagccgc ccctgcggcg tcagattctt gaaggcggtg tcgagcgggc tggtgtccag
      841 cgcaccgtgc gcggccaccc gcagcgattc gtccatcggc tcgtccatcg accggcctcc
      901 ccgtagcctg tttttccgtc aaaacggcta acattttatc agacaattga caatcggaaa
      961 cgcggcgact atagcgctta tttgtcagac ttatttgccg acaatagcga agatgaaaac
     1021 gtccccatgc gcgccggagc ggcggcgcgg gaccggatcg gaatcctcct taccctggag
     1081 gcgcaacctg ccccctccgc ttcggcgaag ggggcttttt tgcgcgccgt ttagagcgtt
     1141 tccccaaaaa gtggctccac tttttggata aggaagatag aaaaaacaaa tacttatagc
     1201 gccaatctga tgcaatcaga tcggcgctct aagcctcagg gctggatgac cggccagccg
     1261 ctgctgaaat cgagactgtc gacgaacagg taatcgaggc cgttctgcgc cagcttcaac
     1321 gcatggaagg cgatcacgcc gtcgccggag ccgtcgacat aggcggtctg gccgccgggg
     1381 ccgctccagg cgctattgcc ttgcaacagg atcgtcccgc cgcccgcggc catgtcggcg
     1441 ccgccctggt cgaggaacgg cccgttcggc gaggtcgagc gcccgacgac gatcttgtaa
     1501 ttgctgttgg ccggactgct ttcgcagcag aaatcccacg agacgaacag gtagtaatag
     1561 ccgcccttat agacgatgga cgcgccctcg accgggtcgt aggtcacgct ggttgcgcgg
     1621 cgcgccagat gatacgcggt cccgcccgtc ttgatcagcc cggtgaccgg gtcgatttcc
     1681 tgctggaaga tcccttccca gaagctgccg tagctcagcc agaccttgcc gccggcgtcg
     1741 acgaggatgt tcgggtcgat ggcgttgaaa ttgctcgacg cctgcgaggt cagcacctgc
     1801 cccttgtcga cccatttata ggccgggtcc gtcggatcca gcgtcgtatt ggtcgccatg
     1861 ccgatggccg agacgttcga gccgaaggtc gagacggcgt aatagaggtg gtagaggccg
     1921 ttgaagtacg aaacgtccgg cgcccagatg gtggtcgcgc ccggaaccgc gccgctgatc
     1981 caggcgggca gggtcgtgaa cacgaagccg caatcgctcc aggccagctt gtcggaactg
     2041 caccgcaccg gaatgaagcc gccctgcgtc gccgaggcgt cggtgacata gacgtaccat
     2101 ttgccgcccg cctgaatgat cgacgggtca tgcaccgggc tgacgtcgcc gctgagcttg
     2161 caactgtcca gaacgcccgc cgaactggag gaggaactgg acgaactgga cgaggaactg
     2221 gaggacgacg aactcgacga cgaggcgctg ctgctggagg acgagcctgt cgaggacgaa
     2281 ccgccgccac cgccgccgcc accgccgcag gcggacagga tggcggcggc ggcgagacag
     2341 gcggcggcga gcgccggacg cgaaggcgtg cgtgtcatgc gaaatctccc tgaataacgg
     2401 cgcttttgtc cgtcctttga acgcgcaata tactcagaca taattttttg cctagaggga
     2461 aaatttccgc gcaaccggcg cgcgccagcc gacgcaaaag ctttaatgtc tgaaaataaa
     2521 gcggaaaatc cttaatttcc gcaacgtaaa gcgccctcgc ccgaaaaatc cggcaagcgc
     2581 catttcgcgc ttgtcgcccc agggcaagaa tgatacacga tttttgtctg agtaaagcgc
     2641 ccttcaaaaa acgcgccgcc ggacaagg
//
LOCUS       DASVLL010000420         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_296742,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000420 DASVLL010000000
VERSION     DASVLL010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296742"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VF402_06975"
     CDS             complement(<1..1058)
                     /locus_tag="VF402_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7800075.1"
                     /translation="MLIIGAGLAGLSAALACRDAGLDFRVLEAGAAPGGRAQTKRVGP
                     GLPADMGGHWLHGEDSPLRALIERYRLPARPDAGAFFISENGHLHASDDDWLTRVIDG
                     DLARRIRLGEAPDVPLPDLAKDGEGRVRLEDFGYLWNGLEPPLRPSAREYLTDENTPG
                     GLQLGGGVGALIEALASDIGYARIRCNTAVTHVVAISDSVCARASDGTSWLARRAIFT
                     GSLAVLTSGMVRFDPPLSDATRAHLDGMVMGEMNKIVIAVDHAFLRERGVAPDTAYEL
                     LDARPPHFCHVHSGGAPLIQLYACGERARRIERLNADQAVGYVSALLRHIPALAGFEA
                     HMHGPPLISTWSANLFTQG"
     gene            complement(1087..1263)
                     /locus_tag="VF402_06980"
     CDS             complement(1087..1263)
                     /locus_tag="VF402_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800076.1"
                     /translation="MTKSPIQSFIDGFNAKPRQQPHHPRLQEDRRMRDNLDEDKVDEM
                     VDDTFPASDPPATY"
     gene            complement(1387..>1700)
                     /locus_tag="VF402_06985"
     CDS             complement(1387..>1700)
                     /locus_tag="VF402_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7800077.1"
                     /translation="QSGVHIDAIAANNDEMALGAIAALQKAGYPAGKVAVGGVDGTAA
                     GLAAIKSGYLTMSLLQNAREQAAEAMADVKKLAQGQYAQLYDWVPYEIIIPSNLATFE
                     H"
BASE COUNT          255 a          566 c          606 g          273 t
ORIGIN      
        1 ccctgcgtga acaggttggc gctccaggtc gagatcagcg gcgggccgtg catgtgcgcc
       61 tcgaacccgg ccagagcggg aatatgacgc aaaagggcgc tgacatagcc gacggcctga
      121 tcggcgttga gccgctcgat ccgccgcgcg cgctcgccgc aggcgtaaag ctggatcagc
      181 ggcgcgccgc cgctgtggac atggcagaag tgcggcgggc gcgcgtcgag cagttcgtag
      241 gccgtgtccg gcgccacgcc gcgttcgcgc aggaaggcgt gatcgaccgc gatgacgatc
      301 ttgttcatct cgcccatgac catgccgtcc agatgcgcgc gcgtcgcgtc ggacagcggc
      361 gggtcgaacc tcaccatgcc cgatgtcagc acggccagag agccggtgaa gatcgcgcgc
      421 cgcgccagcc aactcgtgcc gtcggaggcg cgcgcgcaga cgctgtcgct gatggcgacc
      481 acgtgggtga cggcggtgtt gcagcggatg cgcgcatagc cgatgtcgct ggccagcgcc
      541 tcgatcagcg ccccgacgcc gccgccgagt tgcagcccgc cgggcgtgtt ctcgtcggtc
      601 aggtattcgc gcgccgaagg ccgtagcggc ggctccagcc cgttccacag atagccgaaa
      661 tcctccagcc ggacgcggcc ctccccgtcc ttcgccagat cgggcagggg aacgtccggc
      721 gcctcgccca gccggatgcg gcgggccaga tcgccgtcga tgacgcgcgt cagccagtcg
      781 tcgtcgcttg catgcaaatg gccgttttcg ctgatgaaga aggcgcctgc gtcggggcgc
      841 gcgggcaggc ggtaacgctc gatcagcgcg cgcagcgggc tgtcctcgcc gtgcagccag
      901 tggccgccca tatcggcggg caggccgggg ccgacgcgct tcgtctgtgc gcggccgccc
      961 ggcgcggcgc cggcttcgag cacgcggaag tcgagcccgg cgtcacggca ggccaaggcg
     1021 gcgctcaggc ccgccagccc ggcgccgata atcagcacgt cggtcatcat ggcgacggcg
     1081 acccgttcag taggtcgcgg gcggatcgct ggccgggaag gtgtcgtcga ccatctcgtc
     1141 gaccttgtcc tcgtcgagat tgtcgcgcat ccgcctgtct tcctgcaggc gcggatggtg
     1201 cggctgctgg cgcggcttgg cgttgaagcc gtcgataaag ctctggatcg gtgatttggt
     1261 catggtggac ccctgtgggc gaacccctgt tacggccata atcgggccgt cgggggagca
     1321 taccaccggc gcgataagcc aggggtcacg aatacggggg cgcggcgtaa agacgcggta
     1381 aggccctcag tgttcgaagg tggcgaggtt ggacgggatg atgatctcgt agggcaccca
     1441 gtcataaagc tgggcgtact gaccctgcgc cagcttcttg acgtcggcca tcgcctcggc
     1501 ggcctgttcg cgggcgtttt gcagcagcga catggtcaga tagccggact tgatcgccgc
     1561 cagaccggcc gccgtgccgt cgacgccgcc gaccgccacc ttgcccgccg gataaccggc
     1621 cttttgcagc gcggcgatgg cgcccagcgc catttcgtcg ttattggccg cgatggcgtc
     1681 gatatgcacg ccgctttgca
//
LOCUS       DASVLL010000421         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_349095,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000421 DASVLL010000000
VERSION     DASVLL010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349095"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VF402_06990"
     CDS             complement(<1..805)
                     /locus_tag="VF402_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX7800078.1"
                     /translation="MNANLRNFALWVIIVLLLLALFTLFQNPGQRTTAQDISFSQLLT
                     EVDQGRVRDVVIQGPEIHGTFANGTSFQTYAPSDPGLVQKLYTKGVAITARPPSDNVP
                     WFVSLLVSWLPFIALIGVWIFLSRQMQGAGGKALGFGKSRAKLLAEAHGRVTFDDVAG
                     VDEAKQDLQEIVEFLRDPGKFQRLGGRIPRGVLLVGPPGTGKTLIARAVAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGL"
     gene            complement(938..2020)
                     /gene="tilS"
                     /locus_tag="VF402_06995"
     CDS             complement(938..2020)
                     /gene="tilS"
                     /locus_tag="VF402_06995"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7800079.1"
                     /translation="MAMASDHGEPVSAEEAASLFEDLVAIPALILAVSGGPDSTALLW
                     LAARWRAARTSGPTLIAVTVDHGLRKEAGAEAKAVAKFARHLGIAHRTLRWTGKKPKT
                     GLPQAARMARYRLLAEAARKAGAPAILTAHTLDDQAETVLIRMSRGSGLTGLAGMARV
                     SPVPGRRDGAVRLIRPFLDIAKSRLIATLKAAKIPFADDPTNRDPRFTRARLRGLLPV
                     LAGEGLDAPRLATLARRLARAEAALAQAAAEAFDRLAEEKSGRIAFDRSGFAGLSPEI
                     ALRLLGRAIDALGDEGPVELGKLEGLSGALATALADGQGRFRRTLAGAVVTLTATAIM
                     VERAPPRRSRALTKRRASRRRGGKRR"
BASE COUNT          359 a          764 c          740 g          335 t
ORIGIN      
        1 cgaggccggc gccgcgatgg cggccgaccg cgtcgatttc gtcgatgaag atgatgcaag
       61 gcgcgttctt cttcgcctgc tcgaacatgt cgcgcacgcg tgacgcgccg acgccgacga
      121 acatctcgac gaagtcggag cccgaaatgg tgaagaacgg cacgttggct tcgccggcca
      181 ccgcgcgcgc gatgagcgtc ttgccggtgc ccggaggacc gacgagcagc acgccgcgcg
      241 gaatgcgtcc gccgaggcgc tggaatttgc ccgggtcgcg caggaattcg acgatctcct
      301 gcaaatcctg cttggcctcg tcgacgccgg cgacatcgtc aaatgtcacg cggccgtgcg
      361 cttcggccaa cagcttggcg cgcgacttgc cgaagccgag cgccttgccg cccgcgccct
      421 gcatttgccg cgacaggaag atccacacgc cgatgagcgc gatgaagggc agccacgaca
      481 cgagcagcga cacgaaccac ggcacgttgt cggacggcgg ccgcgcggtg atcgcgacgc
      541 ccttggtata gagcttctgc accaggcccg gatcggatgg cgcataggtc tggaacgacg
      601 tgccgttggc gaaggtgccg tgaatctccg gaccctggat gacgacgtcg cgcacgcggc
      661 cctgatcgac ctcggtcaga agctgcgaga aggagatgtc ctgggcggtg gtccgctggc
      721 ccggattctg gaagagcgtg aacagcgcca gcaacaggag gacgatgatc acccagagag
      781 cgaaattgcg aagattggcg ttcatctaag cgtccttacc cgccgcgaaa gaccatggcc
      841 gcggccgtcc cgtttatgac gaatctttat ggcgacgtca cgtcaacgca atgtatgcac
      901 ggcccttccg catggaaagg gtccgatttc cagtaatcta gcgccttttg ccgccccttc
      961 gccggctcgc ccggcgcttg gttaaggcgc ggctgcgccg cggcggggcg cgttcgacca
     1021 tgatggccgt ggcggtgagc gtcaccaccg ccccggccag ggtccgccga aaccggcctt
     1081 gcccgtcggc caaggcggtc gcaagcgccc ccgagagccc ctcgagcttg ccgagttcga
     1141 ccggtccttc gtcgccgagg gcgtcgatgg cgcggccgag caggcgcagg gcgatttcgg
     1201 gggaaaggcc ggcaaatccg gaccggtcga aggcgatccg gccggatttc tcctccgcaa
     1261 gccggtcgaa ggcctcggct gccgcctgtg ccagcgccgc ctcggcgcgg gcgagccgtc
     1321 gcgccaaagt cgccaaccgc ggcgcatcga gcccctcccc ggccaggacc ggcagcaggc
     1381 cgcgcagccg cgcccgcgtg aagcgcgggt cgcggttggt cgggtcgtcg gcaaagggga
     1441 ttttcgcagc cttcagcgtg gcaatcagcc gcgatttcgc aatgtccagg aaggggcgga
     1501 tgagccggac ggcaccatcc cggcgtcccg gcaccggcga cacccgcgcc atccctgcga
     1561 gccccgtcag cccgctgccg cggctcatgc ggatcagtac tgtctcggcc tgatcgtcca
     1621 acgtatgcgc cgtgaggatc gccggcgcgc cggccttccg cgccgcctcc gccagcagcc
     1681 gatagcgcgc catgcgcgcc gcctgcggca ggcccgtctt cggcttcttg cccgtccagc
     1741 gcagggtgcg gtgcgcgatg ccgagatggc gcgcgaactt cgccaccgcc ttcgcctcgg
     1801 cgcccgcttc cttgcgcaat ccgtgatcga cggtgacggc gatcagcgtc gggccggacg
     1861 tgcgcgccgc gcgccagcgc gccgcgagcc acagcagcgc ggtcgagtcc gggccgccgg
     1921 acaccgccag tatcaaagcc ggaatcgcga cgagatcttc aaaaagcgaa gctgcttctt
     1981 ccgcagaaac cggctcgccg tggtcagaag ccatcgccat cgcccgccgc cggacgttcc
     2041 cggccggcac atcctagacg ttcaggcgct ggcgctgaac cattctccgt tcattcccgc
     2101 gcaagcggga atccagttga gggcgtcagc agtggacgcg cttctgttcg gccgcgaccg
     2161 agcgcttcac gctggccgag gcgtgcggat atttgcgg
//
LOCUS       DASVLL010000422         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_81726, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000422 DASVLL010000000
VERSION     DASVLL010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81726"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VF402_07000"
     CDS             complement(<1..1468)
                     /locus_tag="VF402_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7800080.1"
                     /translation="MLKRGASTLALAACLLSMPAAHAFAQAETPAPAASVRDVADAKA
                     EALVAQMTLDEKIGQLVNVAPAIPRLNIPAYNWWTESLHGALGAVPTTNFPEPVGLAA
                     SFDAPLVKDVAGAISTEVRALHTLGRQTGHLGHIGTGLDTWSPNINIFRDPRWGRGQE
                     TYGEDPYLTAHLGVAFIEGMQGPNPDLPYVIATPKHFAVHSGPEPTRHVADIFASAHD
                     IEDTYLPAFRAAIVEAKAGSVMCAYNSVWGQPACASDLLLKDHLRGDWGFKGYVVSDC
                     DAVTDISIHHKYAPDPAAAVAAAFKAGVDNECNTQTLSDTPGLGARYKEAYERGLLGM
                     NDIDRALVRLFSARFRNGDLPGVRADAPVPVSAINTPEHQALALKAAIESLVLLKNDG
                     VLPFKANARIAVIGPLADATRVLRGNYSSPASAPPISVLAGLRQAMPDARIAYAPAAP
                     SITDGNPIPASAFVTPDGKPGLQAAYYNASGVGADGKAT"
     gene            1756..>2276
                     /locus_tag="VF402_07005"
     CDS             1756..>2276
                     /locus_tag="VF402_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEX7800081.1"
                     /translation="MTINDMRLSRRRLLTASASLTVIPLTGHAADAPPSPLRLWYRQP
                     ATRWEEALPLGNGRLGVMVFGRVAQERLQLNEDTLWSGGPYSADNPDALAALPQVRAL
                     IAAGDYKAATDLASAKMMGKPLSQMSYGAAGDLLLSFDGARAPETYERDLDLQTAVAT
                     TRLRMGGARLTRE"
BASE COUNT          367 a          741 c          801 g          367 t
ORIGIN      
        1 ccgtggcctt gccgtcggcg ccgacgccgg aggcgttgta ataggcggcc tgcaaaccgg
       61 gcttgccgtc cggcgtgacg aaggccgagg ccggaatcgg attgccgtcg gtgatcgagg
      121 gcgcggcggg cgcataggcg atccgcgcgt ccggcatggc ctgacgcaag ccggccagca
      181 ccgagatcgg cggcgcgctg gcgggcgagg aatagttgcc gcgcagcacg cgcgtcgcgt
      241 cggccagcgg cccgatcacg gcgatcctcg cattggcctt gaacggcagc acgccgtcgt
      301 tcttgagcag caccagactc tcgatggcgg ctttgagcgc cagcgcctga tgttcgggcg
      361 tgttgatggc gctgaccggc accggcgcgt cggcgcgcac gcccggcagg tcgccgttgc
      421 ggaaccgcgc cgaaaacagc cgcaccagcg cccggtcgat gtcgttcatg cccagcaggc
      481 cgcgctcata ggcttccttg tagcgcgcgc cgaggcccgg cgtgtcggac agggtctggg
      541 tgttgcactc gttgtccacg cccgccttga aggccgccgc gaccgccgcc gccggatcgg
      601 gcgcgtactt gtgatggatc gagatgtcgg tgacggcgtc gcagtccgac acgacatagc
      661 ccttgaagcc ccagtcgccg cgcaaatggt ccttgagcag caggtcgctg gcgcaggcgg
      721 gctggcccca gacgctgttg taggcgcaca tgaccgaccc ggccttcgcc tcgacgatgg
      781 cggcgcggaa ggcgggcaga taggtgtcct cgatgtcgtg ggccgaggcg aagatgtcgg
      841 cgacgtggcg cgtcggctcc ggcccggaat ggacggcgaa gtgcttgggc gtggcgatga
      901 cgtagggcag gtcggggttc ggcccctgca tgccctcgat gaaggcgacg ccgagatgcg
      961 cggtcaggta cggatcctcg ccgtaggttt cctgcccccg gccccagcgc ggatcgcgga
     1021 agatgttgat attgggcgac catgtgtcca gccccgtgcc gatatggccg agatggccgg
     1081 tctgacgccc cagtgtgtgc agggcgcgca cctcggtgct gatcgcgccc gccacgtcct
     1141 tgaccagcgg cgcgtcgaag ctggcggcca ggccgaccgg ttcggggaag ttggtggtcg
     1201 gcaccgcgcc gagcgcgccg tgcagcgatt cggtccacca gttgtaggcc gggatgttca
     1261 ggcgcggaat cgcgggcgcg acattgacga gctggccgat cttttcgtcc agcgtcatct
     1321 gcgccaccag cgcctcggcc ttggcgtcgg cgacgtcgcg caccgaagcg gccggggccg
     1381 gcgtttcggc ctgcgcgaag gcgtgcgccg ccggcatgga gaggagacag gccgcgaggg
     1441 ccagcgtcga ggcgccgcgt ttcagcatga cgatatttcc ctgtttttat gcattggtcg
     1501 cgccgtttct gatagcgcta ccagacgtta gcccaaaagt cggacaagtg aaagatgttt
     1561 ttacaggcga atcctctccg cctcaaaggc cgtaatccgc ttttattttc aatatcttaa
     1621 cgcccatcca agccgcgcga tcacgcaatg ttgcaaatta tttaacttgc aggccgcgcc
     1681 gcaacgcagt ttagtctgac aataaaagct gtgcgttaaa gtcagccgcg ccgcaaggcg
     1741 taacagggaa ccaagatgac aatcaatgac atgcgcctca gtcggcgccg cctgctgacc
     1801 gccagcgcaa gcctgacggt gataccgcta acaggtcacg ccgccgacgc gccgccgtcg
     1861 ccgctgcggc tgtggtaccg ccagcccgcg acccgctggg aggaggcgct gccgctcggc
     1921 aacggacggc tgggcgtcat ggtgttcggc cgcgtggcgc aggagcggct gcaactgaac
     1981 gaggacaccc tatggtcggg cggtccctat tcggcggaca atcccgacgc cctggccgcg
     2041 ctgccgcagg tgcgcgccct gatcgcggcg ggcgattaca aggcggccac cgatctggcc
     2101 agcgcgaaga tgatgggcaa gccgctgagc cagatgtcct acggcgcggc gggcgacctg
     2161 ctgctcagct tcgacggcgc gcgggcgccg gagacttacg agcgcgacct cgacctgcaa
     2221 accgccgtcg ccacgacgcg cctgcgcatg ggcggcgccc ggctgacgcg cgaaag
//
LOCUS       DASVLL010000423         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_458140,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000423 DASVLL010000000
VERSION     DASVLL010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458140"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VF402_07010"
     CDS             <1..477
                     /locus_tag="VF402_07010"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEX7800082.1"
                     /translation="WLNLPAENKRQPALARDIAAADIPSVIYPKAEAKVLAGRLQGLM
                     GPIMSPPTQPSLLDIQLGPEGEITLPLPADHRGFVYVFSGGVAVEQTLINAGQAGVLT
                     AEGGDTLNLAAGQDGARALVATALPLNEPLVRYGPFAMNGHDGVKQAFTDYQNGLF"
     gene            complement(532..1869)
                     /locus_tag="VF402_07015"
     CDS             complement(532..1869)
                     /locus_tag="VF402_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannanase"
                     /protein_id="HEX7800083.1"
                     /translation="MSSPTRRALMGAGLAAAALGVSLPARAQTAASDFVSRDGPHFRL
                     KGETYRYVGANIWYGAYLGSAASFGDRKRLVKELDDLKALGVGNLRVLGSSELSPLIN
                     SLDPAFSYGPGKYNDDLLSGLDFLLAEMGKRDMKAVLYLTNFWEWSGGMVTYQYWTNG
                     GHYINMNDPAHPWPEFADFSAQFYASGAAKRAYWDYVGMLTNRVNALTGKAYKDDPAI
                     MAWQLCNEPRPGGSDAAVLANSEAYYGWIADTAALIRRNAPNHLVSLGHEGLMGANMH
                     EDILIKAHEHIDYLTAHIWPQNWSWVDKDDLAGSFDAGAAKVQTYIDAHIRIARRIGK
                     PLVFEEFGFPRDNIAYDPGTPTTFKDRFYGLIYKAVEDAIVSDTPVAGSNFWAWGGAG
                     RALHADHHMLRGETAYVGDPPHEPQGWYSVFNTDTSTQSLIKAHAARLAALKA"
     gene            complement(2078..2962)
                     /locus_tag="VF402_07020"
     CDS             complement(2078..2962)
                     /locus_tag="VF402_07020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7800084.1"
                     /translation="MPDLVEHVSDTAFWVAWHRAQEGRRRDALFHDPLAEKLAGERGR
                     RIARHMGIARAMAWSMALRTHSLDGFIRDAVQDGVDMIVNLGAGLDTRPYRLDLPAGL
                     MWVEVDFAPMIAYKNDALADETPVCRLERIACDLSKDAARRDMLTRLNARARHILVLT
                     EGVVPYLDNDQVAALAADLAAQDHVSVWLTDYYSPLFRSLSARGKIMKRLARNAPFRF
                     DPGDAPDDWPRFFAAHGWRIDAVRFTGEEGRRLGRDLPLNWLMRRMMRFAPEARLAPY
                     RRMNGMVRLTRGHDIRPL"
     gene            3072..>3802
                     /locus_tag="VF402_07025"
     CDS             3072..>3802
                     /locus_tag="VF402_07025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX7800085.1"
                     /translation="MTAAALDILKTIYGYDAFRGPQARIVEHVIAGNNAFVLMPTGGG
                     KSLCYQIPAIARPGMGLVVSPLLALMADQVAALRQAGVKAAALNSDLAGEARGALWRD
                     VFNGDLDLLYVAPETLLREDVLERLSQAQLSLIAIDEAHCLSQWGHDFRPSYRQLDAL
                     VRQFPDTPRMALTATADEPTRAEILSHLDIAESDAFIAGFDRPNIRYAVEEKDNPRAQ
                     LKDFLKKHEGESGIVYCLSKRKTDET"
BASE COUNT          675 a         1240 c         1235 g          652 t
ORIGIN      
        1 tggctgaacc tgcccgcgga gaacaagcgc cagccggcgc tggcgcgcga catcgccgcc
       61 gcggatattc cgtccgtcat ctatccgaag gccgaggcga aggtgctggc cgggcgtctg
      121 caaggcctga tggggccgat catgtcgccg ccgacccagc cgtcgctgct ggatattcaa
      181 ctggggccgg agggtgagat caccctgccg ctgcccgcag atcaccgggg cttcgtctat
      241 gtgttcagcg gcggcgtcgc ggtcgagcag accctgatca acgccgggca ggcgggcgtc
      301 ctgacggcgg agggcggcga cacgctcaac ctcgccgccg ggcaggacgg cgcccgcgcg
      361 ctggtcgcca cggccttgcc gctcaacgaa ccgctggtgc gctacggacc gttcgcgatg
      421 aacggccatg acggcgtcaa gcaagccttt accgattatc agaacggcct cttctgattt
      481 tcggtctggc cgcgccgcgt ctctcccccc tgagcggcgc ggcctctttt atcaggcctt
      541 cagggccgcc agacgcgcgg cgtgcgcctt gatcaggctc tgcgtcgacg tgtcggtgtt
      601 gaacacgctg taccagccct gcggctcgtg cggcgggtcg ccgacatagg ccgtttcccc
      661 gcgcagcatg tggtgatcgg cgtgcagggc gcgtccggcg ccgccccatg cccagaagtt
      721 cgagcccgcc accggcgtat ccgaaacgat ggcgtcctcg accgccttgt agatcaggcc
      781 gtagaaccgg tccttgaagg tggtcggcgt gccgggatcg taggcgatgt tgtcgcgcgg
      841 aaagccgaat tcctcgaaca ccagcggctt gccgatccgg cgcgcgatgc ggatatgcgc
      901 gtcgatatag gtttgcacct tggccgcccc ggcgtcgaaa ctgcccgcca gatcgtcctt
      961 gtcgacccag ctccagttct gcggccagat gtgcgcggtc agataatcta tgtgttcatg
     1021 ggcctttatc aggatatcct catgcatgtt ggcgcccatc agcccctcat ggcccagcga
     1081 caccagatgg ttgggcgcgt tgcggcggat cagcgcggcg gtgtccgcga tccagccgta
     1141 ataggcttcg gagttggcca gaaccgcggc gtcgctgccg cccggacgcg gctcgttgca
     1201 aagctgccag gccatgatcg ccggatcgtc cttgtacgcc ttgccggtga gcgcgttgac
     1261 gcggttggtc aacatgccga catagtccca ataggcccgt ttggccgcgc cgctggcgta
     1321 gaattgcgcg gaaaaatccg cgaactccgg ccacggatgc gccgggtcgt tcatgttgat
     1381 gtaatggccg ccattggtcc agtactgata ggtgaccatg ccgcccgacc attcccagaa
     1441 attggtcagg tacaaaaccg ccttcatgtc gcgcttgccc atctcggcca gcaggaaatc
     1501 cagcccgctc agcaggtcgt cattgtactt gcccggccca taggaaaagg ccggatcgag
     1561 cgaattgatc agcggcgaca attcggacga gccgagcacg cgcagattgc cgacgcccag
     1621 cgccttcagg tcgtccagtt ccttcaccag ccgcttgcgg tcgccgaagc tggcggccga
     1681 gccgagatag gcgccgtacc agatgttcgc gccgacatag cgataggttt cgcccttcag
     1741 ccggaaatgc ggcccgtcgc gcgacacgaa gtccgaagcg gcggtttgcg cccgcgcggg
     1801 caggctcacg cccagcgccg cggcggccag acccgcgccc atcagcgcgc ggcgggtagg
     1861 tgacgacatg gctttcctct cctgctgtgt tgtatcgctg agagaggcta gccgataatc
     1921 gcgattaagc aaattcaaaa taattattgc cgcggtagcg ccgagtccta ccggaacgcg
     1981 ggcgcggtaa ggccgggtag ggccgtcgcg cacgcggcga atcgcccgac ctgcgccgcc
     2041 agtatcggcg gccggaggtt gcgcaaaggt taagacatca tagcggacgg atatcgtggc
     2101 cgcgcgtcag ccgcaccatg ccgttcatgc gccgataggg cgcaaggcgc gcttccggcg
     2161 cgaaacgcat catccgccgc atcagccagt tgagcggcag atcgcgcccg agacggcggc
     2221 cttcctcgcc ggtgaaacgc accgcgtcga ttcgccagcc atgcgcggcg aaaaagcgcg
     2281 gccagtcgtc gggcgcgtcg ccgggatcga agcggaacgg cgcgttgcgc gccaaccgct
     2341 tcatgatctt gccgcgcgcc gacaggctgc ggaacagcgg gctgtaatag tcggtgagcc
     2401 agaccgagac atgatcctgc gcggccaggt cggcggccag cgcggcgacc tgatcgttgt
     2461 cgagataggg aacgacgccc tcggtcagga ccaggatatg acgggcgcgg gcgttcagcc
     2521 gcgtcagcat atcgcgccgc gccgcatcct tgctcaggtc gcaggcgatg cgctccagac
     2581 ggcaaaccgg cgtttcatcg gccagcgcgt cgttcttgta ggcgatcatc ggcgcgaaat
     2641 cgacctcgac ccacatgaga ccggcaggca ggtcgagacg atagggccgc gtatcgagcc
     2701 cggcgccgag attgacgatc atgtcgacgc catcctgaac ggcgtcgcgg atgaagccgt
     2761 ccagactatg ggtgcgcaac gccatggacc acgccatcgc gcgcgctatg cccatatgcc
     2821 gcgcgatcct gcggccgcgt tcaccggcca gcttttcggc cagcggatcg tgaaacagcg
     2881 cgtcgcgccg ccgcccttcc tgcgcgcgat gccacgcgac ccagaaggcg gtgtcggaaa
     2941 catgttcgac gagatcaggc atgagcgaaa accggtctgt taaaggcgca gagagattag
     3001 gtgacacaca gatcataatt atggtgaaac ggctattgaa gacctcgctt cgattctcca
     3061 tgctgacgac gatgaccgct gccgctctcg acatccttaa aaccatctac ggctacgacg
     3121 cgttccgggg gccgcaggcg cgcatcgtcg agcatgtcat cgcgggcaac aacgccttcg
     3181 tgctgatgcc gacggggggc ggaaagtcgc tctgctacca gattccggcc atcgcccggc
     3241 ccggcatggg gttggtcgtg tcgccgttgc tggccttgat ggccgatcag gtggcggccc
     3301 tgcggcaggc gggcgtgaag gccgccgcgc tcaattccga cctggcgggc gaggcgcgcg
     3361 gcgccttatg gcgcgacgtc ttcaacggcg atctcgacct gctctatgtc gcgccggaga
     3421 cgctgctgcg cgaggacgtg ctggagcggt tgtcgcaggc gcaactgtcg ctgatcgcca
     3481 tcgacgaggc gcattgcctg tcgcaatggg ggcacgattt ccgtccctcc taccgccagc
     3541 tcgacgcgct ggtccggcag tttcccgata cgccgcgcat ggcgctgacg gcgacggcgg
     3601 acgaaccgac gcgcgcggaa atcctgtcgc acctcgacat cgccgaaagc gacgccttca
     3661 tcgccggttt cgaccggcct aatatccgtt acgctgttga agaaaaagac aatccacgcg
     3721 cccaactcaa ggattttctc aagaagcatg agggcgagag cggcatcgtc tactgcctgt
     3781 cgaaacgcaa gaccgacgag ac
//
LOCUS       DASVLL010000424         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_349215,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000424 DASVLL010000000
VERSION     DASVLL010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349215"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VF402_07030"
     CDS             complement(<1..1242)
                     /locus_tag="VF402_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7800086.1"
                     /translation="MHMGPTEIFLIAMLIILTAPYLVWRLFRTDDYAPLVVVQIIGGI
                     LLGPGVLGAVFPAYYRFVFNPQTVGALNGVAWWAVMIFVFVAGIELDLKKAWRHRVET
                     GVTAGLALGTPLVCGCAAALGVLALARHTGGAALWIGPKGQTWQFILGIGMACAVTAL
                     PILVLFLEKLSILRQPLGQRLLRYASLDDVAIWGVLALILLDWARVGRQAGFLAAFFV
                     AAWLTRKLMRRVREGDRWYLALIWLAACGFAADWSGLHFMVGAFLAGAALDADMFDQK
                     KMDLFREHILLAVMPVFFLSTGLRTTWEMGGAAVFAAAGALLVASVAGKLAGVHAAGR
                     ILKWEKGEASIIGWLLQTKALIMIIFVNVLLDKGIITSGAFTATLLMALGSTMLSIPM
                     TKPLLTRYGAIVFKTAEATPDA"
     gene            1440..1691
                     /locus_tag="VF402_07035"
     CDS             1440..1691
                     /locus_tag="VF402_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX7800087.1"
                     /translation="MPTMTSREFNQDVGRAKRDAREEPVFVTDRGKPTHVLMSFDAFC
                     RLSGQGESILDLLALDDGAQIDLEASSLGAGWDRREAFR"
     gene            1691..2191
                     /locus_tag="VF402_07040"
     CDS             1691..2191
                     /locus_tag="VF402_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7800088.1"
                     /translation="MYLLDTGIVAALRKAKWDADLTPLAQWARRVPNPNLFISALSLL
                     ELENGVAQAAKRDPEAAKPLRDWLDAQVARAFDGRILSLDAAIVRRRAQLSYEDGRDG
                     LMAATALEHGLTLVTRRPSAYRSGRVKLLNPFDAETQDSLLDKEQWPGDARAGASWLR
                     NLFLRF"
BASE COUNT          416 a          799 c          688 g          324 t
ORIGIN      
        1 cgcgtccggc gtcgcctcgg ctgtcttgaa gacgatggcg ccatagcgcg tcagcagcgg
       61 cttggtcatc gggatcgaca gcatggtcga gcccagcgcc atcaggagcg tggccgtgaa
      121 ggcgccgctg gtgatgatgc ccttgtcgag caggacgttg acgaagatga tcatgatcag
      181 cgccttggtc tgcaacagcc agccgatgat cgacgcctcg cccttctccc atttcaggat
      241 gcggcccgcg gcgtgaaccc ccgccagctt gcccgccacc gacgcgacca gcagcgcgcc
      301 cgccgccgcg aacaccgccg cgccgcccat ctcccacgtc gtgcgcaggc cggtcgacag
      361 gaagaagacc ggcatgacgg ccagcaggat atgttcgcgg aacaggtcca tcttcttctg
      421 gtcgaacatg tccgcgtcga gcgccgcgcc cgccaggaag gcgccgacca tgaagtgcag
      481 gcccgaccag tccgccgcga aaccgcacgc cgccagccag atcagcgcca gataccagcg
      541 gtcgccctcg cggacgcgcc gcatcagctt gcgcgtcagc cacgcggcga cgaagaaggc
      601 ggcgaggaaa ccggcctgac gcccgacccg cgcccagtcg agcaggatca gcgccagcac
      661 gccccagatc gccacatcgt ccagcgaggc gtaacgcagc aaccgctggc ccagcggctg
      721 acgcaatatg ctcagctttt cgaggaacag caccagaatc ggcagcgccg tcaccgcgca
      781 ggccatgccg atgccgagaa tgaactgcca cgtctggccc ttcggcccga tccacagcgc
      841 cgccccgccc gtatggcgcg ccagggccag cacgcccagc gccgccgcgc agccgcacac
      901 gagtggcgtc cccagcgcca gccccgccgt cacgcccgtc tcgacgcgat gccgccaggc
      961 ctttttcagg tccagctcga tgcccgccac gaagacgaag atcatcaccg cccaccacgc
     1021 gacgccgttc agcgcgccga cggtctgcgg attgaagacg aaacggtaat aggccggaaa
     1081 gacggcgccc agcacgcccg gccccagaag gatgccgccg atgatctgca ccaccaccag
     1141 cggcgcgtaa tcgtcggtcc tgaacagccg ccacaccaga tatggcgcgg tcaggatgat
     1201 cagcatggcg atcaggaaaa tctcggtcgg acccatatgc ataggcgtgt cgctccccgc
     1261 gtgcgttgtg attttccgcc acgaagccgc aggccggaac gcgggtcaag ccgaaacaga
     1321 aaattgcaac tcatttattt tattgatgat ttttatgttg caatgccgcc acatcgtagg
     1381 catgttgcta caaataaccg tgatttcgac actacgttcc tgcaaaacgg aggcccgcca
     1441 tgcccaccat gaccagccgc gaattcaatc aggacgtcgg ccgcgccaag cgcgacgcgc
     1501 gcgaggagcc ggtgttcgtc accgaccgcg gcaagccgac ccatgtgctg atgagcttcg
     1561 acgccttctg ccgcctgagc ggtcagggcg aatcgatcct cgacctgctg gcgctggacg
     1621 acggcgcgca gatcgatctg gaggcttcca gcctcggcgc gggctgggac cggcgcgagg
     1681 cgttccgctg atgtacctgc tcgataccgg cattgtcgcg gccttgcgca aggccaagtg
     1741 ggacgccgac ctgacgccgc tggcgcaatg ggcgcggcgc gtccccaatc ccaacctgtt
     1801 catctccgcc ctgtccctgc tggagctgga gaacggcgtc gcgcaggcgg cgaagcgcga
     1861 ccccgaagcc gccaagccct tgcgcgactg gctggacgcg caggtcgccc gcgccttcga
     1921 cggccgcatc ctcagcctcg acgccgccat cgtgcgccgc cgcgcgcaac tcagctacga
     1981 ggacgggcgc gacggcctga tggcggcgac ggcgctggag cacggcctga ccctggtgac
     2041 gcgccgcccg tcggcctacc gctccgggcg cgtgaagctg ctcaacccgt tcgacgccga
     2101 aacgcaggac tcccttctgg ataaggaaca atggcccggc gacgcgcgcg cgggcgcaag
     2161 ctggctgcgc aacctgttct tacgctttta gataattagc cagcacgacg gcgttgttgt
     2221 gcgcctc
//
LOCUS       DASVLL010000425         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_241769,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000425 DASVLL010000000
VERSION     DASVLL010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241769"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VF402_07045"
     CDS             complement(<1..433)
                     /locus_tag="VF402_07045"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEX7800089.1"
                     /translation="MAQGDLPAPVKTRAYAPNRIPSAPPVAADTPVRLSPDTAATPSH
                     QTWYPAGQPANLAAPAAKSAVSSQPVVMNVRIGEHQDRTRFVIELSDPVKLRVFTLVN
                     PNRVVIDMPEVLWRLNGPQKPTTNGAIKRYRYGLFRPGNARF"
     gene            933..>1789
                     /locus_tag="VF402_07050"
     CDS             933..>1789
                     /locus_tag="VF402_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800090.1"
                     /translation="MKRMLIDASHREETRVVVLDGSKVEEFDFEAASKRPLKGNIYLA
                     KVTRVEPSLQAAFVEYGGNRQGFLAFNEIHPDYYQIPIADRQALLEEQQREARRHAEE
                     EGFESVETGTAVVSDEELEAEEQAEAAAGTAEEIPSENGEEPVEDEAHAETEPHDDED
                     FFDNKGGDHVAESEVESGFDVEQADLAATDEEDEDEHPHLDGEHDHEDDDENPLEQLA
                     DEEVDEGIVDQQTLSEAGDAPHAEAHGEEHQYPYSAEPPHIVDPNVESDQGIVDEQAA
                     AESFVEGEQ"
BASE COUNT          409 a          562 c          541 g          277 t
ORIGIN      
        1 cgaaccgcgc attgccagga cggaacaagc catagcgata acgtttgatc gcgccgttag
       61 tggtgggttt ctgaggcccg ttcaaccgcc agagcacctc gggcatgtcg atgacgacgc
      121 ggttcggatt gacgagcgtg aacacccgca gcttcaccgg atcggaaagt tcgatgacaa
      181 agcgcgtgcg gtcctggtgc tcgccgatac gcacattcat cacgaccggc tgcgaagaga
      241 cggcgctttt tgcggccggc gcggcgagat ttgcgggctg tccggcggga taccaggtct
      301 gatgcgaggg ggtggcggcc gtatccggag acagccggac cggggtatcg gccgcaaccg
      361 gcggcgcgga gggtatccgg ttgggggcgt aagcccgagt tttaacgggg gccggcaagt
      421 ctccctgggc caaggctatt ccggcccaac ccagtaccaa aactccaaaa acagccccaa
      481 aacgcctcat aacggccttt cgctacccaa aaccggccaa atcaagacca agtcaaactg
      541 cctccaaacc atttacggtt cgttagccgc ttcggatttt acaatgattc cgaacctaac
      601 ccttgccgga cccgcgcggg atacctaaaa ggaaaacaac ctgcttcgcc ggatttcggc
      661 gattccgcac tcggccccgg gccgagcctg cagggcgata ggcgcccgca gacccccaaa
      721 tccgcaacca gcaggtgccc tcaggcgaac ggcctagccg caagccacaa agtgaattga
      781 tatgaccagc ttcgccagag ccctcgcgcc agtccaagcg gcccagccgc cggtggtgac
      841 gctccggctt tccacccatc acgaaacaat gcggctccat ccgatgcagc gtgccctgag
      901 ggcgcgccgc ggtgcgccgc ccggagattt ccatgaaacg catgttgata gacgccagcc
      961 accgggaaga gacccgcgtg gttgttctcg acggttcgaa ggtcgaggaa tttgatttcg
     1021 aggccgcgtc caagcggccg ctcaagggaa acatctatct tgccaaggtg acgcgggttg
     1081 agccgtcgct gcaggcggcg ttcgtggagt atggcggcaa ccgccagggc ttcctcgcct
     1141 tcaacgaaat ccatcccgac tattatcaga tcccgatcgc cgatcgtcag gcgctgctcg
     1201 aagaacagca gcgcgaggct cgccgccacg cggaagaaga aggctttgaa tcggtcgaga
     1261 ccggcaccgc cgttgtctcc gatgaagaac tcgaagccga agaacaggcc gaagctgccg
     1321 cgggcacggc cgaggaaatt ccgtccgaga atggcgaaga gccggtcgag gatgaggccc
     1381 acgccgaaac cgagcctcac gacgacgaag atttcttcga caacaagggc ggcgatcacg
     1441 tcgccgaaag tgaggtcgaa tccggtttcg acgtcgaaca ggccgatctt gccgcaaccg
     1501 acgaggagga cgaggacgaa catcctcatc tcgacggcga acacgatcac gaagacgacg
     1561 acgagaatcc gctcgaacag ctcgcggatg aagaagtcga cgaaggcatc gtcgatcagc
     1621 agaccctctc cgaagccggc gatgcgccgc atgccgaagc ccatggcgaa gaacaccagt
     1681 atccctacag cgccgagccg ccccacatcg tcgatcccaa tgtcgaatcc gatcagggca
     1741 tcgtggacga acaggccgcc gcggagtcct ttgtggaagg cgagcagca
//
LOCUS       DASVLL010000426         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_188547,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000426 DASVLL010000000
VERSION     DASVLL010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188547"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(428..504)
                     /locus_tag="VF402_07055"
     tRNA            complement(428..504)
                     /locus_tag="VF402_07055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(468..470),aa:Pro,seq:ggg)
     gene            complement(589..1086)
                     /locus_tag="VF402_07060"
     CDS             complement(589..1086)
                     /locus_tag="VF402_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7800091.1"
                     /translation="MSKGPLALRTISETADILGVPQHVLRFWETRFTFIRPTKRAGGR
                     RFYRPQDIELLTGVKTLLYDRGYTIRGVQKLFKDQGQKAVLGAHDATALPTGQGLVGE
                     DEIAAENAAGAAQPVFGAGEGGPLVTDEMRARFLDIARQLDEAQATLGRSLGRDTNRG
                     KFSRE"
     gene            complement(1105..1407)
                     /locus_tag="VF402_07065"
     CDS             complement(1105..1407)
                     /locus_tag="VF402_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX7800092.1"
                     /translation="MKSQTLTRAELFEDVHNELGLPRQECAAIVERTLDLMVEALERG
                     ETVKLSGFGVFQVRDKNARVGRNPKTGKPAAIDPRKVISFRASQIVKARVDAGVEA"
     gene            complement(1505..2476)
                     /locus_tag="VF402_07070"
     CDS             complement(1505..2476)
                     /locus_tag="VF402_07070"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7800093.1"
                     /translation="MRTAVTGVGAYLPEEVVTNADLAKVVDTSDEWIANMTGIRERRR
                     AAPEQASSDLAVEAARKALAAAGRTPEDLDLIIVATTTPDLTFPATAALVQKKLGAPM
                     GIAFDVQAVCSGFVYALSVADGFVARGLAKCVLVIGAEAMTKLLDFSDRSTCILFGDG
                     AGAVVLEPVEGEGTTDDRGILGFDLRCDGSKTDLLYVSGGVFETENRIGKIKMQGNQV
                     FKQAVQKISQAIEAAIEKADLKIEDIDWFIPHQANQRILNGVAHHLGLDEGKVISTVA
                     MHANTSAASIPLAWYEGVRDGRVRPGDLVLIEALGGGLTWGAAAIRL"
BASE COUNT          437 a          845 c          765 g          445 t
ORIGIN      
        1 atcgggtgat gtgcgggcat gaacggctcc tgagggtcaa tagattcgac cggagcgttt
       61 cccgcgttca ggagccgcac ggacgaacga caggataccg acggcagcat ccgatgttcg
      121 tcgcggggat cccggcgcga ggccaatgac cagagctttc gttaccgaaa ggacttgggc
      181 ttaccaaacc aagtcagcct tttccgacgt ggcggatata gcattgagaa ggttgggggg
      241 caatgggctg tcgttgaata cggtgtcgct gcgcgacgag tcttaagttg agcgacagga
      301 ttggctccac ttcgtttcgc cgaactcgcc acttcgtggc tcgtttcgaa cgaggttcga
      361 acatgaacgc tccgaccatt tgattataga aaaggccagc ccaagggctg gccttttcta
      421 tatcagatgg tcggagcgac aggattcgaa cctgcgaccc cttgaccccc agtcaagtgc
      481 gctaccaggc tgcgccacgc tccgtccatg acctgaaaaa ggcgaaagcg cctcatcagg
      541 agacgcggct tatagagacg aaacctcaca gccgcaatcc ccaaaagctc actcgcgcga
      601 gaatttcccc cgatttgtgt cgcgccccag cgaccggccc agggtcgcct gcgcctcgtc
      661 cagttgccgg gcgatatcga ggaaacgcgc gcgcatctca tcggtcacaa gcggtccgcc
      721 ctcaccggcg ccgaacaccg gctgcgccgc cccggcggcg ttttccgccg cgatctcgtc
      781 ctcgccgacc agcccctgcc cggtcggcag cgccgtcgcg tcatgcgcgc ccaaaaccgc
      841 cttctggccc tgatccttga acagcttctg cacgccccgg atcgtgtagc cgcgatcgta
      901 gagcagcgtc ttcacgccgg tcagcagttc gatatcctgc gggcggtaga accggcgccc
      961 gcccgcgcgc ttggtcgggc ggatgaaggt gaacctggtt tcccagaaac gcagcacatg
     1021 ttgcggcacg cccagaatat cggcggtctc ggatatggtc cgtaaggcta aaggcccctt
     1081 gctcaccgcg gtttacgcct ccgactaagc ttccacgccc gcatcgacgc gcgccttgac
     1141 gatctgcgag gcgcggaacg agatcacctt gcgcggatcg atggcggcgg gcttgccggt
     1201 cttggggttg cgcccgacgc gcgcgttctt gtcgcgcacc tggaacacgc cgaagcccga
     1261 cagcttgacc gtttcgccgc gctccagcgc ctcgaccatc aggtccagcg tgcgctcgac
     1321 gatagcggcg cattcctgcc gcggcaaacc cagctcgtta tgaacgtcct cgaacagttc
     1381 cgcgcgcgtc aaggtctgcg atttcatggc gtccatgctc cacctgtgct gtagcagagt
     1441 cttaacatgc cgcaaatccg ccttgtcgtc aaaggcttat tcacaagagt tcagaaggcg
     1501 cgccctacag ccggatcgcc gccgcgcccc aggtcaaccc gccgcccagc gcctcgatca
     1561 gcaccagatc gcccggcctg acgcgtccgt cgcgcacgcc ctcgtaccag gccagcggaa
     1621 tcgacgccgc cgaggtgttg gcgtgcatgg ccacggtcga gatcacctta ccctcatcga
     1681 ggccaagatg atgcgcgacc ccgttcagga tgcgctgatt ggcctgatgc gggatgaacc
     1741 agtcgatgtc ctcgatcttc aggtccgcct tttcaatggc cgcctcgatg gcctgcgaaa
     1801 tcttctgcac ggcctgcttg aagacctgat tgccctgcat cttgatcttg ccgatgcggt
     1861 tctccgtctc gaacacgccg ccggagacgt acagaaggtc ggtcttggag ccgtcgcagc
     1921 gcaggtcgaa acccagtatg ccgcggtcgt ccgtcgtccc ctcgccctcg accggctcca
     1981 gcaccaccgc cccggcgccg tcgccgaaca ggatgcaggt ggagcggtcg gaaaaatcga
     2041 gcagcttcgt catcgcctcg gcgccgatca ccagcacgca tttggccagc ccgcgcgcca
     2101 cgaagccgtc ggccacgctc agggcgtaga cgaagcccga gcagaccgcc tgcacgtcga
     2161 aggcgatgcc catgggcgcg ccgagcttct tctggaccag cgccgccgtc gccgggaagg
     2221 tcaggtcggg cgtggtggtg gccacgatga tcaggtcgag gtcctcgggc gtgcgcccgg
     2281 ccgcggccag cgcctttcgc gccgcctcga ccgccaggtc ggacgaggcc tgctccggag
     2341 ccgcccggcg gcgctcgcgg atgccggtca tgttggcgat ccactcgtcg gaggtgtcga
     2401 cgaccttggc caggtccgcg ttggtgacga cctcttcggg gagataggcg ccgacgccgg
     2461 tgacggcggt ccgcagggtc gctgtcactc gg
//
LOCUS       DASVLL010000427         4605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_82318, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000427 DASVLL010000000
VERSION     DASVLL010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82318"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="VF402_07075"
     CDS             <1..1571
                     /locus_tag="VF402_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800094.1"
                     /translation="FDSRVKGDAGAQTPAKARGTATEDMDETPFLGAEFAGGLPVMYR
                     RRPLVQPEDIAAMLPVELGSGVNLYGYYMFHGGRNPEGDTTLEESDLNGGYNGLPVIN
                     YDFQAPYGQYGQPNPVLDLIRPWHEFLNAFGGRLAPMSVHRPEVTPKGRDDLVTPRWA
                     VRSSGRSGFVFLSNHVRQYAMAEQKATRFRVDLPGGALTFPSQPVDIADGASFAWPFH
                     FDLDGVDLVWASAQPVTRLSDGGQVVYVFEAQDGIAPEFAFDRDVEALSGHKTRTGGH
                     VLVSDVTPGRGVAIRAGKARIVVLSARDAQRLSVLDYQGRRRLILSDALAYGDGTDLH
                     LRSENPQFAFGVWPPVTAPRASLPLAPVKTTGLFQTFRAQAAPVSPQATVEPVRPAGT
                     VPPLNIGGRAKAALQPYPETYGQAAAWRIAIAPDALTGTADVTLDIRYRGDVARLFDG
                     AKMVDDQFWYGPDWRVGLRRLGVGPFTLNVLPLRADAPVYIDDAYRPRPSDGAQVADV
                     ASVRLVPTYGLDLR"
     gene            1664..4186
                     /locus_tag="VF402_07080"
     CDS             1664..4186
                     /locus_tag="VF402_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7800095.1"
                     /translation="MSKPLPSEAADMTQARLAAIVDSSNDAIVGKDLNSIITSWNASA
                     ERIFGYAPEEVIGKSITLLIPPELQSEEDVIIGKIRAGERIHHFATERVCKSGRRIDV
                     SITVSPIRDATGRIVGASKIARDISGQKQAEARRAALMRLTDAIRDLEDPDAIAFAAA
                     RVLGETLRISRAGYGVIDTEAETITIERDWNAPGIDSLAGVLRFRDYGSYIEDLKRGR
                     AVVFADAETDPRTAPRAAALKAISAQAVVNLPVTERGRFVALLYLNNATARAWSDAEL
                     DFVREAAERTRTATERARVAAALKQREAELRELNEHLERRVEAALAERRILADIVEST
                     DAFILVCDLSLNLLAINRAGADEFEHIYGVRPKTGDAMLALIARLPREQEAMRAVWSR
                     ALAGEAFAHVGDFGDPERARRHYEMKFNPLFDEEGRQIGAYLFAFDMTERLRDQQRLR
                     TAEDQLRQAQKMEAVGQLTGGVAHDFNNMLAVVSGSLELLDRRTDPEEARSRRLIAAA
                     LEASKRAGTLTQRLLAFSRQQPLKPEVLDPNKLVSGMSDLFRHSLGAHVQLETVLAGG
                     LWRIHADQNQLENVLLNLAVNARDAMPDGGRLTIETQNAHLDQRYVASELGILPGQYV
                     LIAVTDSGAGMTEDVMRKAFDPFFTTKDVGKGTGLGLSQVYGFVKQSGGHIKIYSEVG
                     EGTTIKIYLPRHLGPAEDGGDPGRTETPPPADRGELILVVDDEDLVRRFSVEALTELG
                     YRVLEASSAQAALAMILDRPDVDLLFTDIVMPEMNGKKLADLVREKRPDLPVIYTTGY
                     TRNAVVHNGTLDPDVELISKPFTIEELATRVRTVLEAKAVRR"
     gene            complement(4202..>4605)
                     /locus_tag="VF402_07085"
     CDS             complement(4202..>4605)
                     /locus_tag="VF402_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800096.1"
                     /translation="GFAVFVVPGIMFGVAVCVAIPAYVGEKGRGLWGSVKRSFELTKG
                     HRWTLFLLFFVAIVATWILEVVVEAPFLPSLVASVTRGAQPALGLPLMLLLSAFSALL
                     SLLWYVFFAAIYVSLRESKDKLSPQQTANVF"
BASE COUNT          764 a         1537 c         1624 g          680 t
ORIGIN      
        1 gcttcgacag ccgcgtcaag ggcgacgccg gggcgcagac gcccgcgaag gcgcgcggca
       61 cggcgacgga ggacatggac gagacgccgt ttctgggcgc cgagttcgcg ggcgggctgc
      121 ccgtcatgta ccgccgccgc ccgttggtgc agcctgagga catcgccgcc atgctgccgg
      181 tcgagctggg gtcgggggtc aatctctacg gctattacat gttccacggt gggcgaaacc
      241 ccgaagggga cacgacgctg gaggaaagcg atctgaacgg cggctataac ggcctgccgg
      301 tgatcaatta cgattttcag gcgccttatg gccagtacgg ccagccgaat ccggtgctgg
      361 acctgatccg gccgtggcat gaattcctca acgcgttcgg cggccggctg gcgccgatga
      421 gcgtgcatcg cccggaggtc acgccgaagg ggcgggacga cctcgtcacg ccgcgctggg
      481 ccgtgcgcag ttcgggccgg tccggcttcg tgttcctgag caatcatgtg cggcaatatg
      541 cgatggccga acagaaggcg acgcggttcc gcgtcgacct gcccggcggg gcgctgacct
      601 ttccgtcgca gccggtggac atcgccgacg gggcgtcgtt cgcgtggccg ttccatttcg
      661 acctcgacgg cgtcgatctg gtctgggcca gcgcgcagcc ggtcacgcgc ctgagcgacg
      721 gcgggcaggt cgtctatgtc ttcgaggcgc aggacgggat cgcgccggaa ttcgccttcg
      781 accgcgacgt cgaggctttg tccgggcaca agacgcggac gggtggacat gtcctcgtgt
      841 cggacgtgac gccggggcgc ggcgtggcga tccgggcggg caaggcgcgg atcgtcgtcc
      901 tgtcggcgcg ggacgcgcag cgcctgagcg tgctggacta tcaggggcgc aggcggctga
      961 tcctcagcga cgcgctggcg tacggcgacg ggacggacct gcatctgcgg tcggagaacc
     1021 cgcagttcgc gttcggcgtc tggccgccgg tgacggcgcc gcgggccagc ctgccgctgg
     1081 cgccggtcaa gacgacgggc ctgttccaga ccttccgcgc gcaggccgcg ccggtgtcgc
     1141 cgcaggcgac ggtggagccg gtccgcccgg ccggaacggt tccgccgctc aacatcggcg
     1201 gccgggcgaa ggcggcgttg cagccctacc ccgaaaccta cggtcaggcg gcggcgtggc
     1261 gcatcgccat cgcgccggac gcgctgacgg ggacggcgga cgtgacgctg gacatccgtt
     1321 atcgcggcga cgtggcgcgt ttgttcgacg gcgcgaagat ggtggacgac caattctggt
     1381 acgggccgga ctggcgcgtg gggctgcggc ggctgggcgt tgggccgttc acgctgaacg
     1441 tgctgccgct gcgcgccgac gcgccggtct atatcgacga cgcctaccgc cccaggcctt
     1501 cggacggcgc gcaggtcgcg gacgtggcgt cggtgcggct ggtcccgacc tacgggctgg
     1561 acctgcgata gcgggcggcg gcggtctttc ggactgcttt cttatttttt tggctttacc
     1621 cggagagatg ttgagccgac gcatccctat cacgagtgac gccttgagca agccgttgcc
     1681 ttccgaagcc gccgacatga ctcaggccag gctggccgcc atcgtcgatt cctccaacga
     1741 cgccatcgtc ggcaaggatc tcaacagcat catcacctca tggaacgcca gcgccgagcg
     1801 catcttcggc tatgcgccgg aggaggtgat cggcaagtcg atcaccctgc tgatcccgcc
     1861 cgaactccag tccgaggagg acgtgatcat cggcaagatc cgcgcgggcg aaagaatcca
     1921 ccatttcgcg accgagcggg tgtgcaagtc gggcaggcgc atcgacgtgt cgatcaccgt
     1981 gtcgccgatc cgcgacgcca cgggccgcat cgtcggcgcc tcgaagatcg cccgcgacat
     2041 cagcggccag aagcaggccg aggcgcgccg cgccgccctg atgcggctga ccgacgcgat
     2101 ccgcgacctg gaagaccccg acgcgatcgc cttcgccgcc gcccgcgtgc tgggcgagac
     2161 cttgcggatc agccgggcgg gctatggcgt catcgacacc gaggccgaga cgatcaccat
     2221 cgaacgcgac tggaacgcgc cgggcatcga cagtctggcc ggggtgctgc gtttccgcga
     2281 ctacggctcc tatatcgagg atctgaaacg cggccgcgcc gtcgtgttcg ccgacgccga
     2341 gaccgacccg cgcaccgcgc cgcgcgccgc agcgctcaag gcgatcagcg cgcaggcggt
     2401 cgtcaatctg ccggtgaccg aacgcggccg cttcgtcgcc ctgctttacc tcaacaacgc
     2461 cacggcgcgg gcgtggtccg acgccgagct ggacttcgtg cgcgaggccg ccgaacgcac
     2521 gcgcacggcc accgaacgcg cccgcgtcgc cgccgcgctg aagcagcgcg aggccgaact
     2581 gcgcgagctg aacgaacatc tggagcggcg cgtcgaggcc gcgctggccg agcggcgcat
     2641 cctggccgac atcgtcgaat ccaccgacgc cttcattctg gtctgcgacc tgagcctcaa
     2701 cctgctggcg atcaaccgcg cgggcgcgga cgagttcgag catatttacg gcgtgcggcc
     2761 gaaaaccggc gacgccatgc tggcgctgat cgcgcgcctg ccgcgcgagc aggaggccat
     2821 gcgggcggtg tggagccggg cgctggcggg cgaggccttc gcccatgtcg gcgacttcgg
     2881 cgacccggag cgcgcgcgcc gccattacga gatgaagttc aacccgctct tcgacgagga
     2941 gggccgccag atcggcgcct atctgttcgc cttcgacatg acggagcggc tgcgcgacca
     3001 gcagcgcctc aggaccgccg aggaccagtt gcggcaggcg cagaagatgg aggcggtggg
     3061 ccagttgacc ggcggcgtcg cgcacgattt caacaacatg ctggccgtgg tgtcgggttc
     3121 gctggaattg ctggaccggc gcacggaccc ggaagaggcg cgctccaggc ggttgatcgc
     3181 cgcggcgctg gaggcgtcga agcgcgccgg aacgctcacc cagcgcctgc tggccttttc
     3241 gcgccagcag ccgctcaagc ccgaagtgct cgatccgaac aagctggtgt cgggcatgtc
     3301 cgacctgttc cgccattcgc tcggcgcgca tgtccagttg gagaccgtgc tggccggggg
     3361 attgtggcgc atccacgccg atcagaacca gttggagaac gtgctgctca acctggccgt
     3421 caacgcgcgc gacgccatgc cggacggcgg caggctgacc atcgaaacgc agaacgccca
     3481 tctcgaccag cgctatgtgg ccagcgaact gggcatcctg ccgggacaat atgtgctgat
     3541 cgccgtgacc gacagcggcg cgggcatgac cgaggacgtc atgcgcaagg cgttcgaccc
     3601 cttcttcacc accaaggacg tcggcaaggg caccggtctg ggcctcagtc aggtctatgg
     3661 cttcgtcaag cagtcgggcg gccacatcaa gatctattcc gaggtcggcg aaggcacgac
     3721 gatcaagatc tacctgccgc gccacctcgg ccccgccgag gacggcggcg accccggccg
     3781 cacggaaacc ccgccgccgg cggatcgcgg cgagctgatc ctggtcgtcg acgacgagga
     3841 tctggtgcgg cgcttctcgg tcgaggcgct gaccgagctg ggctaccgcg tgctggaggc
     3901 gtcgagcgcg caggcggcgc tggcgatgat tctggaccgg cccgacgtcg accttttgtt
     3961 caccgacatc gtcatgcccg agatgaacgg caagaagctg gccgacctgg tcagggagaa
     4021 gcgcccggac ctgccggtga tctacacgac cggctatacg cgcaacgccg tggtgcataa
     4081 cggcacgctc gatcccgacg tcgagctgat cagcaagccg ttcaccatcg aggaactggc
     4141 gacgcgcgtg cggacggtgc tggaggcgaa ggccgtcaga aggtaacggc ccgctccaag
     4201 cctagaacac gttggccgtc tgctgcggcg acagcttgtc cttgctctcg cgcaggctga
     4261 catagatggc ggcgaagaag acgtaccaca gcagggagag cagggcgctg aacgcgctga
     4321 gcagcagcat cagcggcagg ccgagcgccg gttgcgcgcc gcgcgtcacg ctggcgacca
     4381 gggacggcag gaagggcgcc tccaccacga cttcgagaat ccatgtggcg acgatggcga
     4441 cgaagaagag caggaacagc gtccagcggt ggcccttggt cagttcgaac gagcgcttca
     4501 cgctgcccca caggccgcgg cccttttcgc cgacataggc cgggatggcg acgcagaccg
     4561 cgacgccgaa catgatcccc ggcacgacga acaccgcgaa gccga
//
LOCUS       DASVLL010000428         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_513120,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000428 DASVLL010000000
VERSION     DASVLL010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513120"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VF402_07090"
     CDS             <1..702
                     /locus_tag="VF402_07090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518935.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7800097.1"
                     /translation="AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDDNGAGS
                     LTALPVIETQANDVSAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSSA
                     QIKAMKQVAGAIKGDLAQYREMAAFAKFGSDLDAATQRLLARGARLTELLKQPQYSPL
                     KVEEQVCVIYAGTRGYLDKVAVSDVGRFEREFLSLLRTRHTDLLDAIRTQKALSSELE
                     EKLKGVVSDFAATFA"
     gene            712..1602
                     /locus_tag="VF402_07095"
     CDS             712..1602
                     /locus_tag="VF402_07095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX7800098.1"
                     /translation="MASLKDMRNQIGSVKATQKITKAMQMVAAAKLKRAQETAENARP
                     YAKHMAAVIANLAKGVSGDSAPQMLVGNGSTYTQLVVVATADRGLAGGFNSNIARAAR
                     EFIGAQIAAGKNVRIICVGRKGRDQLKRLYGDRIVESFDMSGTKVLSMDAVKPIAELI
                     TREFLEGRGDVVTLFYSNFKSVIAQVPASKRLIPADVDLTAAEDTPNSSLGGAVYEYE
                     PGEAELLETLLPRNLGVQLLASMLENNAGFYAAQMSAMDNATRNAGEMINALNLQYNR
                     KRQAQITTELIEIIAGAEAL"
     gene            1615..>2639
                     /gene="atpD"
                     /locus_tag="VF402_07100"
     CDS             1615..>2639
                     /gene="atpD"
                     /locus_tag="VF402_07100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7800099.1"
                     /translation="MSAHQKIAAATGKIAQIIGAVVDVEFDGHLPAILNALETTNGAS
                     GARLVLEVAQHLGENTVRAIAMDATEGLVRGQPVTDLGAPITVPVGPATLGRIMNVIG
                     EPIDEAGPIDTKFFNPIHADAPAFADQATSAELLVTGIKVVDLICPYAKGGKIGLFGG
                     AGVGKTVTIQELINNIAKAYGGYSVFAGVGERTREGNDLYHEMIESKVNELGGGGTSR
                     CTLVYGQMNEPPGARARVALTGLAQAEYFRDVEGKDVLFFVDNIFRFTQAGSEVSALL
                     GRIPSAVGYQPTLATEMGKLQERITSTNKGSITSVQAVYVPADDLTDPAPATSFAHLD
                     ATTVLSRS"
BASE COUNT          494 a          917 c          802 g          426 t
ORIGIN      
        1 gcctatcgcc agatgtcgct gctgctgcgc cgtccgcccg gccgcgaagc ctatccgggc
       61 gacgtcttct atctgcactc ccgcctgctg gagcgcgcgg cgaagctgaa cgacgacaat
      121 ggcgcgggtt ccctgaccgc cctgccggtc atcgagactc aggccaacga cgtgtcggcc
      181 tatatcccga ccaacgtgat ctcgatcacc gacggccaga tcttcctcga aaccgacctg
      241 ttctatcagg gcatccgccc ggcggtgaac gtcggcctgt cggtgtcgcg cgtcggctcg
      301 tccgcccaga tcaaggcgat gaagcaggtc gcgggcgcga tcaagggcga cctggcccaa
      361 taccgcgaaa tggccgcctt cgccaagttc ggctcggacc tcgacgccgc gacgcaacgc
      421 ctgctggcgc gcggcgcccg cctgaccgag cttctgaagc agccgcaata ttcgccgctg
      481 aaggtcgagg agcaggtctg cgtgatctac gccggcacgc gcggctatct cgacaaggtc
      541 gccgtctcgg acgtgggccg tttcgagcgc gaattcctgt cgctcctgcg cacccgtcac
      601 accgatcttc tggacgcgat ccgcacccag aaggcgctgt cctcggaact ggaagagaag
      661 ctcaagggcg tcgtttccga tttcgccgcg accttcgcct aaggttccga catggccagc
      721 ttgaaggata tgcgcaatca gatcggaagc gtgaaagcca cgcagaagat cacgaaagcg
      781 atgcagatgg tggcggcggc caagctcaag cgcgcgcagg agacggcgga aaacgcccgt
      841 ccctacgcca agcacatggc tgcggtcatc gccaatctcg ccaagggcgt ctccggcgat
      901 tccgcgccgc agatgctggt cggcaacggc tcgacctaca cgcagctcgt cgtcgtggcg
      961 acggcggacc gcggtctggc gggcggtttc aactcgaaca tcgcgcgcgc cgcgcgtgag
     1021 ttcataggcg cgcagatcgc ggcgggcaag aatgtccgca tcatctgcgt cggccgcaag
     1081 ggccgcgacc agctcaagcg cctgtacggc gaccgcatcg tcgaaagctt cgacatgtcg
     1141 gggaccaagg tgctaagcat ggacgcggtc aagccgatcg ccgagctgat cacgcgcgaa
     1201 ttcctcgaag ggcgcggcga cgtcgtcacc ctgttctact cgaacttcaa gtcggtgatc
     1261 gcgcaggttc cggcctcgaa gcgcctgatc cccgccgacg tcgatctgac cgccgccgag
     1321 gatacgccca attcttctct tggcggcgcg gtctatgaat atgaaccggg cgaggccgaa
     1381 ctgctggaaa ccctgctgcc gcgcaatctc ggcgtgcagc ttctggcctc catgctggaa
     1441 aacaacgccg gtttctacgc cgcccagatg agcgcgatgg acaacgccac gcgcaatgcg
     1501 ggcgaaatga tcaacgccct caatctccaa tacaaccgca agcgtcaggc ccagatcacg
     1561 accgaactga tcgagatcat cgccggcgcc gaagccctct aaggaacgat acccatgtcc
     1621 gctcatcaaa agatcgcggc tgcgaccggc aagatcgctc agatcatcgg cgcggttgtc
     1681 gacgtcgagt tcgacggcca cctgcccgcc attctcaacg cgctggaaac caccaacggc
     1741 gcgtcgggcg cccgtctggt gctggaagtc gcccagcacc tcggtgaaaa caccgtgcgc
     1801 gccatcgcca tggacgccac cgaaggtctg gtgcgcggtc agcccgtcac cgatctcggc
     1861 gcgccgatca ccgtgccggt cggtccggcg acgctgggcc gcatcatgaa cgtcatcggc
     1921 gagccgatcg acgaagccgg tccgatcgac accaagttct tcaacccgat ccacgccgac
     1981 gccccggcct tcgccgatca ggcgacctcg gcggagctgc tggtcacggg catcaaggtc
     2041 gtcgacctga tctgccccta cgccaagggc ggcaagatcg gcctgttcgg cggcgccggc
     2101 gtcggcaaga ccgtcaccat tcaggaactg atcaacaaca tcgccaaggc ttacggcggc
     2161 tattcggtgt tcgccggcgt cggcgagcgc acccgcgaag gcaacgacct ctatcacgaa
     2221 atgatcgagt cgaaggtgaa cgaactgggc ggcggcggaa cctcgcgctg cacgctcgtt
     2281 tacggccaga tgaacgagcc tcccggcgcc cgcgcccgcg tcgccctgac cggcctcgct
     2341 caggccgaat atttccgcga cgtcgaaggc aaggacgttc tgttcttcgt cgacaacatc
     2401 ttccgcttca cgcaggccgg ttcggaagtg tcggcgctgc tgggccgcat cccgtccgcc
     2461 gtgggctatc agccgaccct ggccaccgag atgggcaagc tgcaagagcg catcacctcg
     2521 accaacaagg gttcgatcac ctcggttcag gccgtgtacg tgcccgccga cgacctgacc
     2581 gacccggcgc cggcgacctc gttcgcccac ctcgacgcca ccacggttct gtcgcgctc
//
LOCUS       DASVLL010000429         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_513186,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000429 DASVLL010000000
VERSION     DASVLL010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513186"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VF402_07105"
     CDS             complement(<1..574)
                     /locus_tag="VF402_07105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7800100.1"
                     /translation="MRGLAAEDSARIRLTRAQGLTLLGVLAALGLAAALAPMATGHVA
                     YMTLWLLFAGNAALRLAAALLPQGAGKTPYLHPGLLPRYTVIVALYHEAGIAAQVLSA
                     MLALRYPKTRLDIVFALEEDDAETIAALSALDLPGHVSLSLTPPGFPRTKPRALNHAL
                     MQARGDLVVVYDAEDRPDPGQLLEAARAFAA"
     gene            881..1603
                     /locus_tag="VF402_07110"
     CDS             881..1603
                     /locus_tag="VF402_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7800101.1"
                     /translation="MSLDECADQYVLGLVERDRVLALSDHARYADSYFRDRAQGIRRI
                     KPRLESVLALHPDMVVRAWGGDLRLIQALQRRGIKVVDIDDVQSYAAARAQLFKVGAA
                     LDAEAGAQIAAHRFDEALSQVRNIGRGRTVIYYTPSGTSAGPDTLTGDMLKTLGFKLA
                     VTRSGYFSISPESLIAMKPDVFALGYYDDRYAMRRVPGRQPLVRALIEKTPHFALPDA
                     AMACGGWFSLYALRDLSQETAP"
     gene            1603..>2153
                     /locus_tag="VF402_07115"
     CDS             1603..>2153
                     /locus_tag="VF402_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX7800102.1"
                     /translation="MNLSLTSLNLWLAGAIVALIALLLLFGDVPLSGAMWLDALIHWK
                     SVGAEIVWGIRLPRNLAAAGVGAMLGMSGALMQGLLRNPLAEPGLLGVSAGAGLGAAV
                     AIVLGLGLIDFAVAGFALIGAVIVAVILLVFVERFPQRQSLILLGVGLSALCGALMAL
                     TFNLSPSPVTTAEMLGWMMGSVE"
BASE COUNT          305 a          774 c          711 g          363 t
ORIGIN      
        1 cggcggcgaa ggcgcgcgcc gcctccagca actgccccgg atcgggccgg tcctcggcgt
       61 cgtacaccac cacgagatcg ccgcgcgcct gcatcagcgc atgattgagc gcgcgcggct
      121 tggtgcgcgg aaagcccggc ggcgtcagcg acagactcac atggccgggc aggtccagcg
      181 cgctcagcgc cgcgatcgtc tccgcgtcgt cctcctccag cgcgaacacg atgtccagcc
      241 gcgtcttcgg ataacgcagg gccagcatgg cgctcagcac ctgcgccgcg atcccggctt
      301 cgtggtaaag ggcgacgatc accgtatagc gcggcagcag gcccggatgc aggtacggcg
      361 tcttgcccgc cccttgcggc agcagcgccg ccgccagccg cagcgcggcg ttgcccgcga
      421 acagcagcca caacgtcatg taggccacat gccccgtcgc catcggcgcc agcgcagccg
      481 ccaaccccag cgccgccagc acgcccaaca gcgtcaggcc ctgcgcgcgc gtcagccgta
      541 tccgcgccga atcctccgcc gccaggccgc gcaaaggcgt gcggcggcgg cgcgtgggcc
      601 gcgcgccggg gaaaagatgg attcggacgg ttccggcggc cttagaatcc ttatagacct
      661 gtcgtcgctc aaaggattcg gtcttaaacg cccggttcat cgcgcccccg ctgacctgtt
      721 ccgttttaga acttcaggtt tatgaaaagc gggttttatt tagaggtgac agcgattgcg
      781 agtagcgtcc gcgctaaaat tactcgccct atgcgcgacc tgcctggcgc ccggcgcggc
      841 gcaggcgcgc gatcccgcgc ctgtttccct gccgaaactg gtgtctcttg acgaatgcgc
      901 cgatcaatat gtgctgggtc tggtcgagcg cgaccgcgtg ctggccctgt ccgaccatgc
      961 gcgctacgcc gactcttact tccgcgaccg ggcgcaggga atccgccgca ttaagccgcg
     1021 cctcgaatcg gtgctggccc tgcatcccga catggtggtg cgcgcctggg gcggcgacct
     1081 gcgcctgatt caggcgttgc agcggcgcgg gatcaaggtg gtcgatatcg acgacgtgca
     1141 gtcctacgcc gccgcccgcg cccagctttt caaggtcggc gccgcgctgg acgccgaggc
     1201 gggcgcgcag atcgccgcgc accgcttcga cgaggccctg tcgcaggtgc gcaatatcgg
     1261 ccgcggccgc acggtcatct attatacgcc gtccgggacc agcgcgggcc ccgacaccct
     1321 gaccggcgac atgctgaaaa ccctcggctt caagctggcc gtgacgcgga gcggctattt
     1381 ttcgatctcg ccggaaagcc tgatcgccat gaagcccgac gtgttcgcgc tcggctatta
     1441 cgacgaccgc tacgccatgc ggcgcgtgcc gggccgtcag cccctggtgc gcgccctgat
     1501 cgaaaagacc ccgcatttcg ccctgcccga cgccgccatg gcctgcggcg gctggttcag
     1561 cctctacgcc ctgcgcgacc tatcccagga gacggcgccc tgatgaacct gtccctcacc
     1621 tcgctcaatc tgtggctggc cggcgcgatc gtggcgctga tcgcgctttt gctcctgttc
     1681 ggcgacgtgc cgctcagcgg cgccatgtgg ctggacgcgc tgatccactg gaagtccgtc
     1741 ggcgccgaga tcgtctgggg catccgcctg ccgcgcaacc tcgccgccgc cggggtcggc
     1801 gccatgctgg gcatgagcgg cgcgctgatg cagggcctgt tgcgcaatcc gctggccgaa
     1861 ccgggcctgc tgggcgtgtc ggcgggcgcg gggctgggcg ccgccgtcgc catcgttctg
     1921 ggcctcggcc tgatcgactt cgcggtcgcc ggattcgcgc tgatcggcgc ggtgatcgtc
     1981 gccgtcatcc tgctggtctt cgtcgagcgc ttcccgcagc ggcaatcgct gatcctgctc
     2041 ggcgtcggcc tgtcggcgct gtgcggcgcg ctgatggcgc tgaccttcaa cctgtcgccc
     2101 tcgccggtca ccaccgccga gatgctgggc tggatgatgg gctcggtcga gaa
//
LOCUS       DASVLL010000430         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_349892,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000430 DASVLL010000000
VERSION     DASVLL010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349892"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VF402_07120"
     CDS             complement(<1..944)
                     /locus_tag="VF402_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7800103.1"
                     /translation="MTQSDDHLFGVYTRAPVVAERGEGVRLYDRSGRMWIDFVQGVAT
                     NAFGHAAPFLVEALKAQAEKVWHVSNIYRIPGQDELAGKLCDRSFADKVFFTNSGTEA
                     VECALKLARKYHAHRGQPERFDVIGMTGAFHGRTYAAVSAAGNPAYLEGFGPALPGYV
                     HVPFGDMDAVREAAARPSAAAIIVEPVQGEGGLRDLTPAQLHELRAICDAHGLLLIFD
                     EIQCGMGRTGKLWAHEYSDIVPDVMTVAKALGGGFPIGACLATEAAAEGMVFGAHGST
                     FGGNPLAMAVGIAAFDALSAPELLDNVNRMAGYLRQQM"
     gene            complement(1104..1310)
                     /locus_tag="VF402_07125"
     CDS             complement(1104..1310)
                     /locus_tag="VF402_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800104.1"
                     /translation="MVHPNFSDQAGGMARRPAPFRMEMDAVRRELMARDQRAAQQRRL
                     TQLAIAAAVAALIGAALWALRGGL"
     gene            complement(1389..2300)
                     /locus_tag="VF402_07130"
     CDS             complement(1389..2300)
                     /locus_tag="VF402_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX7800105.1"
                     /translation="MRNLFKIIAAALLLAFAPRIAAAEAAPTVTVAHPLLWEIDDGAA
                     RVYLFGSIHVMKPDTVWLSDDLQRRFDSAGQAWFEVADLDDQAAVQQQAQRYMLDPSG
                     HMTAGLTPAEVVRLDALLAPYGLGSAQMMGYRKWAVALVLNVQQIAAAGYNPKTGVDL
                     TLLQQARATGKPVHGFETIDQEMQKLAPADDAEDMAALRSAIADSATSAQDIADLFQA
                     WANGDEKRLTSFMVDKMKAESPSLYRRVIVERDAAWTPQVERLVRDLKAQGGGTAFVT
                     VGVGHLVGPDSLIAMLRADGAKVKQVE"
     gene            complement(2302..2493)
                     /locus_tag="VF402_07135"
     CDS             complement(2302..2493)
                     /locus_tag="VF402_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7800106.1"
                     /translation="MKNRIKVLRAERDWSQADLAERLGVSRQTVNAIETEKYDPSLPL
                     AFRIAALFGLAIEAIFTSE"
BASE COUNT          487 a          917 c          932 g          476 t
ORIGIN      
        1 tccatctgct gacgcagata gcccgccatg cggttgacgt tgtcgagcag ctccggcgcg
       61 gacagcgcgt cgaaggccgc gatcccgacc gccatggcca gcgggttgcc gccgaaggtc
      121 gagccatgcg cgccgaacac catgccttcc gccgccgcct cggtggccag gcacgcgccg
      181 atggggaagc cgccgcccag cgccttggcg acggtcatca cgtcggggac gatgtcggaa
      241 tattcatgcg cccacagctt gccggtgcgg cccatgccgc actggatctc gtcgaagatc
      301 agcaggaggc cgtgcgcgtc gcagatggcg cgcagctcgt gcagttgcgc gggcgtcagg
      361 tcgcgcagac cgccttcgcc ctgcaccggc tcgacgatga tcgcggcggc cgagggtctg
      421 gcggcggctt cgcgcaccgc gtccatgtcg ccgaacggca catgcacata gccgggcagg
      481 gccgggccga agccttcgag ataggccgga ttgcccgccg ccgaaacggc ggcgtaggtg
      541 cggccgtgga aggcgccggt catgccgatg acgtcgaaac gctccggctg gccgcgatgg
      601 gcgtggtatt tgcgcgccag cttgagcgcg cactcgaccg cctccgtgcc ggaattggtg
      661 aagaacacct tgtcggcgaa ggagcggtcg cacagcttgc ccgccagttc gtcctgaccc
      721 ggaatacggt agatgttcga gacgtgccag accttttcgg cctgcgcctt cagcgcctcg
      781 accagaaacg gcgcggcgtg gccgaaggcg ttggtcgcca cgccctgcac gaagtcgatc
      841 cacatgcggc ccgatcggtc atacagccgg acgccttcgc cgcgctcggc caccacgggc
      901 gcgcgcgtat agacaccgaa cagatgatcg tcagactggg tcatggcttg atccttgccg
      961 ttgatttgaa acaggaaagc ggccgcatcg cgggatgcgg atacaaggtg cgcctaagct
     1021 gtggggggcc taggcgcgcc ttgtgtataa aatcagccgg aaagtcaatg aaatccttgg
     1081 accggagccc cgaacgccgc cgctcagagg ccgccgcgca gcgcccacag ggccgcgccg
     1141 atcagcgcgg cgaccgccgc cgcgatggcc agttgcgtca gccgccgctg ctgcgccgcc
     1201 cgctggtcgc gcgccatcag ttcgcgtcgc accgcgtcca tctccatccg gaacggcgcg
     1261 ggccgccgcg ccatgccgcc ggcttggtcg gaaaaattcg gatggaccat aatgggacgc
     1321 ctttcgaaca gggacggagg ttgcaatccc gccgtgcaaa tccggggcca gtcccaccgc
     1381 gccggttgtt actcgacctg tttcaccttg gcgccgtcgg cgcgcagcat ggcgatcagg
     1441 ctgtccggcc cgaccagatg gccgaccccg accgtgacga aggcggttcc gccgccctgc
     1501 gccttgaggt cgcggacaag ccgctcgacc tgcggcgtcc aggcggcgtc gcgctcgacg
     1561 atgacgcgac gatagagcga cggcgactcg gccttcatct tgtcgaccat gaaagaggtc
     1621 agccgctttt cgtcgccgtt cgcccaggcc tggaacaggt cggctatgtc ctgggccgag
     1681 gtcgccgaat ccgcgatcgc ggaccgcagc gcggccatgt cctcggcgtc gtcggcgggc
     1741 gccagctttt gcatctcctg atcgatggtc tcgaagccgt gcaccggctt gcccgtggcg
     1801 cgcgcctgtt gcagcaaggt caggtcgacg ccggtcttcg gattgtagcc ggcggcggcg
     1861 atctgctgca cgttcagcac cagcgcgacc gcccatttcc gatagcccat catctgcgcg
     1921 gaaccgaggc cgtagggcgc cagcagcgcg tcgagcctga cgacctcggc gggggtcagc
     1981 ccggcggtca tatggccgga cgggtccagc atatagcgct gcgcctgttg ctgcacggcg
     2041 gcctgatcgt caagatcggc gacctcgaac cacgcctgac cggcgctgtc gaaacgcctt
     2101 tgcaggtcgt cgctgagcca gaccgtgtcc ggcttcatga cgtggatcga gccgaacaga
     2161 tagacgcgcg ccgcgccgtc gtcgatctcc cacaacaaag gatgcgcgac ggtgacggtg
     2221 ggcgccgctt cggcggcggc gatgcgaggc gcgaaggcca gcaacagcgc ggcggcgatg
     2281 atcttgaaca ggttgcgcat gttattccga agtgaaaatg gcttcgatgg cgaggccgaa
     2341 cagcgccgcg atccggaagg ccagcggcag ggaagggtcg tacttttccg tttcgatagc
     2401 gttgacggtc tgacgcgaga cgccgaggcg ctcggccaga tccgcctgcg accagtcgcg
     2461 ctcggcgcgc aagaccttga tgcggttctt catgaccgtt cccgcgcgcg cggcggcgcg
     2521 gcgcgtttgt ccgcgcgcat cagcggtatt tccacaggac ggcggccagc gccgcgagtc
     2581 cgaaacgaca ggtccatgat gacgacggca tgtgcggggc tcccggcgcg atatgacaag
     2641 gatgctttac atattggaga cggttttgtc aagcaagctg gacattatgc acttgacata
     2701 cctatatttt catcacgctt tttcatttac caaccataaa atgaaaaaaa ctaatcagaa
     2761 ttcttgtctg ggcgttgcct ttgatgtatt gatgcatcat ggaacagaat cg
//
LOCUS       DASVLL010000431         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_242275,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000431 DASVLL010000000
VERSION     DASVLL010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242275"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF402_07140"
     CDS             <1..543
                     /locus_tag="VF402_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800107.1"
                     /translation="DAEVARARQDFGQDALVLKPRISAGAKNTLVWDGERLADAVATD
                     AKAVPPVGPAMIQPYMPAIASEGEWSLIFFVGEFSHAVLKTPKAGDFRSQPDYAARLR
                     AMAPPPEALRLAQGCVDFIGRDRLLYARVDMVRAPDGGFCLMELELIEPDLYLTYDRC
                     ASMRFKDAVERALHVGCGRH"
     gene            complement(697..1338)
                     /gene="nth"
                     /locus_tag="VF402_07145"
     CDS             complement(697..1338)
                     /gene="nth"
                     /locus_tag="VF402_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7800108.1"
                     /translation="MRQPSPALIKKLFERFEQDKPEPKTELDFVNPYTLVVAVALSAQ
                     TTDKAVNKATEPLFKIADTPQKMLDLGEDRLMRMIASIGLYRNKAKNVIALSRLLIDR
                     YNGQVPLTREGLLALPGVGNKTASVVLNELGVEPAIAVDTHVFRVSHRLGLVDADATT
                     PDKVERQLMAVIPKKWLTRAHHWLILHGRYVCTARNPKCLSCIVSDLCPKIGL"
     gene            1413..>1794
                     /locus_tag="VF402_07150"
     CDS             1413..>1794
                     /locus_tag="VF402_07150"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX7800109.1"
                     /translation="MTAILFVCLGNICRSPLAEGVFRHQAEAAGVADRFRIDSAGCGG
                     WHAGEAPDPRAIAAAARHGIHISGQSARQIGPADFDAFDLILGLDRDNVRHLQRMAGQ
                     GSGTARVTLYLEEALGLGKDTPDPY"
BASE COUNT          296 a          564 c          589 g          345 t
ORIGIN      
        1 gacgccgagg tcgcccgcgc gcgtcaggat ttcgggcagg acgcgctggt gctgaaaccg
       61 cgcatctcgg cgggggccaa gaacacgctg gtctgggacg gcgaacggct ggccgatgcc
      121 gtcgccacgg acgccaaggc cgtaccgccg gtgggaccgg cgatgatcca gccctacatg
      181 cccgccatcg cgtccgaagg cgaatggtcg ctgatcttct tcgtcggcga attttcccat
      241 gcggtgctga aaaccccgaa ggcgggcgat ttccgttcgc agccggatta cgccgcgcgc
      301 ctgcgcgcga tggcgccgcc gccggaggcg ctgcgactcg cgcaaggctg cgtcgatttc
      361 atcggccgcg accggctgtt gtacgcacgc gtcgatatgg tgcgcgcgcc cgatggcggc
      421 ttttgcctga tggagctgga gttgatcgag ccggaccttt acctgaccta cgataggtgc
      481 gcctcgatgc gtttcaagga cgcggtcgaa cgggcgctgc atgtcggatg tgggcgccat
      541 tgattttttg agttttttag ccacgaaaag cacgaaagga cacgaaaggg tttttgcgcg
      601 aagcgcgctc tattctccat cgtcagtaaa aaagaacgcg cttcgcgcag gactctattt
      661 tcgtgtcctt tcgtgccttt cgtggcaaaa aaaaccttac aacccgatct tcggacacag
      721 atcgctgacg atgcagctca aacacttcgg attgcgcgcc gtacagacat agcggccatg
      781 caggatcagc cagtggtgcg cgcgggtcag ccattttttc gggatgaccg ccatcagttg
      841 ccgctccacc ttgtcgggcg tggtcgcatc cgcatccacc agacccaacc gatgcgagac
      901 gcggaagaca tgcgtgtcga ccgcgatggc cggttcgacg cccagttcgt tcagcaccac
      961 gctggccgtc ttgttgccga cgccgggcag ggccagcagc ccctcgcgcg tcagcggaac
     1021 ctgaccgtta tagcggtcga tcaggaggcg gctcagggcg atgacgttct tcgccttgtt
     1081 gcggtaaagc ccgatggagg cgatcatgcg catcagcctg tcctcgccga ggtcgagcat
     1141 cttctgcggc gtgtcggcga ttttgaacaa gggttcggtc gccttgttga ccgccttgtc
     1201 cgtcgtctgg gccgacaggg cgacggccac caccagcgtg tagggattga cgaaatcgag
     1261 ttcggtcttc ggttcgggct tgtcctgctc gaaacgctcg aacagctttt tgatcagggc
     1321 ggggcttggc tgtctcatgg cggcatcatg ggcgaaaagc gttgctgcgg caagggcttg
     1381 ccgcgcggga cggcggcctg taagacgcgg gcatgaccgc gatcctgttc gtctgccttg
     1441 gcaatatctg ccgctcgccg ctggccgagg gcgtgttccg ccatcaggcc gaggccgccg
     1501 gcgtggccga ccgtttccgg atcgattccg ccgggtgcgg cggctggcat gcgggcgaag
     1561 cgcccgaccc gcgcgcgatc gccgccgccg cgcggcacgg gatccacatt tccggccaga
     1621 gcgcgcgcca gatcggaccc gccgacttcg acgccttcga cctgatcctc ggcctcgacc
     1681 gcgacaatgt ccgccatttg cagcgcatgg cgggccaggg ttcggggacg gcgcgggtga
     1741 cgctctatct tgaggaagcg ctcggcctcg gcaaggacac gcccgatccc tatt
//
LOCUS       DASVLL010000432         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_27981, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000432 DASVLL010000000
VERSION     DASVLL010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27981"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="gshB"
                     /locus_tag="VF402_07155"
     CDS             <1..627
                     /gene="gshB"
                     /locus_tag="VF402_07155"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX7800110.1"
                     /translation="KTLVVNNPTSVRDAPEKLLATHFPDLQPPTLVSADPVAIHDFFA
                     RHGDCILKPLHGAAGNGVIKLKAGDRNLDALIELFGSISREPVILQKFIPAVSAGDKR
                     IILIDGEIAGGVNRVPEEGAVRSNLRVGGRAERLELTARDREICARLKPVLKNKGLMF
                     VGIDVIGDYLTEINVTSPTGAVQLKAFEGVDAGDLLWDRIEARHAAKG"
     gene            840..2390
                     /locus_tag="VF402_07160"
     CDS             840..2390
                     /locus_tag="VF402_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX7800111.1"
                     /translation="MPARVTTVAFEGAEARRVEAEVVLTNGHIAFQVVGLGDKAVAES
                     RERVRGAFAGMGLALPGKRIVANLSPADLPKEGTHFDLPIALALMTEMGVIPQDALDR
                     YVCVGELRLDGSIAAIAGALPAAIAASSYGMGLICPHDNGTEAAWAGDVSILAPRSLI
                     GLINHFKGQAVLSPPAPGEIRTARKAMDLREVKGQETPKRALEIAAAGGHNLLFVGPP
                     GSGKSMLAQRLPGILPPLTSRELLETSQIWSMAGLIAKGELTRERPFRSPHHSASMAA
                     LTGGGLRARPGEASLAHNGVLFLDELPEFSPQALDSLRQPLETGDVVVARANNHVRYP
                     ARVQLVAAMNPCRCGYGGVGKGACGKAPRCQSDYQNRVSGPLMDRIDLQIDVPPVTAM
                     DMALPAPAEGSAEVAARVAAARELQAERAGRLDLAPDNALNATAYGAGLEAICALDDG
                     ARALLTKAAEQASLSARGWTRTLRLARTIADLEQSGATLRRHVAEALIYRRRSREGAT
                     EARLYTPS"
     gene            2467..>2912
                     /locus_tag="VF402_07165"
     CDS             2467..>2912
                     /locus_tag="VF402_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7800112.1"
                     /translation="MSALPAPSDHDLSPEDRLKAREDFFRLMSHEIRTPLNGVMGMLS
                     LLSRTPLSPDQESYLDTARESGEHLLTLVNDLLDYMRIDAGRIQLEPTQVRLEPLLQS
                     VAELLSPRAHAGGIEIAWALDSRLEHIQADEGRLRQILLNFAGNAV"
BASE COUNT          482 a          977 c          976 g          477 t
ORIGIN      
        1 aagacgctgg tggtcaacaa tccgaccagc gtgcgcgacg cgccggaaaa gctgctggcg
       61 acgcatttcc ccgacctgca accgccgact ttggtcagcg ccgatccggt cgccatccac
      121 gacttcttcg cgcgccacgg cgactgcatc ttaaagccgc tgcacggcgc ggcgggcaac
      181 ggggtgatca agctcaaggc gggcgaccgc aatctcgacg cgctgatcga actgttcggc
      241 tccatcagcc gcgagccggt catcctgcaa aaattcatcc cggcggtcag cgcgggcgac
      301 aagcgcatca tcctgatcga cggcgagatc gcgggcggcg tcaaccgtgt gccggaggag
      361 ggcgcggtgc gctccaacct gcgcgtcggc gggcgggcgg aacggctgga actgaccgcg
      421 cgtgaccgcg aaatctgcgc gcggctgaaa cccgtgctga agaacaaggg cctgatgttc
      481 gtcggcatcg acgtaatcgg cgactatctg accgaaatca acgtcacctc gccgaccggc
      541 gcggtgcagc tcaaggcgtt cgaaggggta gacgcgggcg atcttttgtg ggatcggatc
      601 gaggcgcgcc acgccgccaa gggttgagcg cggcgcgtcc ggccttcaca aatctttaac
      661 aatttcaggg atttgcacaa ttattgcgtg cgcgaagttt gaaattcccc tattgttcga
      721 tttttgttct tgatttgtat ttttatcccc gctaacttgt ggatagattc tgtatctcgt
      781 caccgtattc cggtcgcgaa catcatacaa tttctgtagc gcagcatagg ggggatgtca
      841 tgcccgcgcg agtcaccacc gtagcgttcg agggcgccga ggcgcgccgc gtcgaggccg
      901 aggtcgtgct gaccaacggc catatcgcgt ttcaggtcgt gggcctgggc gacaaggcgg
      961 tggccgaaag ccgcgagcgg gtgcgcggcg ccttcgcggg catggggctg gccctgccgg
     1021 gcaaacgcat cgtcgccaat ctgtcgcccg ccgacctgcc caaggaaggc acgcatttcg
     1081 acctgccgat cgcgctggcg ctgatgaccg agatgggcgt gatcccgcag gacgcgctcg
     1141 accgctatgt ctgcgtcggg gaattgcggc tcgacggctc gatcgcggcc atcgccgggg
     1201 ccttgcccgc cgcgatcgcg gcctcgtcct acggcatggg gctgatctgc ccgcacgaca
     1261 acggcaccga ggcggcgtgg gcgggcgacg tctcgatcct ggcgccgcgt tcgctgatcg
     1321 gcctgatcaa ccatttcaag ggacaggccg tgctgtcgcc gcccgcgccc ggcgaaatca
     1381 ggacggcgcg caaggcgatg gacctgaggg aggtcaaagg gcaggaaacg cccaagcgcg
     1441 cgctcgagat cgcggcggcg ggcgggcata atcttctgtt cgtcggcccg cctggatcgg
     1501 gcaagtcgat gctggcgcag cgcctgcccg gcatattgcc gccgctgacc tcgcgcgaac
     1561 tgctggagac ctcgcagatc tggtcgatgg ccgggctgat cgccaagggc gagctgacgc
     1621 gcgagcggcc gttccgcagc ccgcaccatt cggcctcgat ggcggccctg accggcggcg
     1681 gcctgcgcgc gcggccgggc gaagcgtcgc tggcgcataa cggcgtgctg tttctcgacg
     1741 aactgccgga attttcgccg caggcgctcg attcgctgcg tcagccgctg gagaccggcg
     1801 acgtggtggt ggcgcgcgcc aacaaccatg tgcgctatcc ggcgcgcgtg caactggtcg
     1861 cggcgatgaa tccgtgccgc tgcggctacg gcggcgtcgg caagggcgcg tgcggcaagg
     1921 cgccgcgctg ccagagcgat taccagaacc gcgtctccgg ccccctgatg gaccgcatcg
     1981 acctgcagat cgacgttcca ccggtgaccg cgatggacat ggccctgccc gcgcccgccg
     2041 aaggctcggc cgaggtcgcc gcgcgcgtgg cggcggcgcg cgaacttcag gccgaacgcg
     2101 ccgggcggct cgatctggcg ccggacaacg cgctcaacgc caccgcctac ggcgcggggc
     2161 tggaggcgat ctgcgcgctc gacgacggcg ccagggcgct tttgaccaag gccgccgagc
     2221 aagcgtcgct gagcgcgcgc ggctggacgc gcaccctgcg gctggcgcgc accatcgccg
     2281 atctcgaaca gtcgggcgcc accttgcgcc gccacgtcgc cgaagccctg atctaccgcc
     2341 gccgaagccg cgaaggcgcg acggaggcgc ggctctatac cccttcttaa cctccgtttg
     2401 gcgcagcctt aaccttttgc ggttaagctg cggaacatca gaacgatcac gacggaaaat
     2461 ccgcccatgt cagccctgcc cgctccctcc gatcacgatc tgtcgccgga agaccggctc
     2521 aaggcgcgcg aggatttctt ccggctgatg agccatgaaa tccgcacccc gctcaacggc
     2581 gtgatgggca tgttgtcgct gctgtcgcgc acgccgctgt cgccggatca ggaatcctac
     2641 ctcgatacgg cgcgcgagtc gggcgaacat ctgctcaccc tggtcaacga cctgctcgac
     2701 tacatgcgca tcgacgccgg gcgcatccag ctggagccga cgcaggtccg gctggagcct
     2761 ctgctgcaaa gcgtggccga gcttctgtcg ccgcgcgccc acgcgggcgg catcgagatc
     2821 gcctgggcgc tcgattcgcg tctggagcat attcaggccg acgagggccg cctgcgccag
     2881 atcctgctga acttcgccgg caacgccgtg aa
//
LOCUS       DASVLL010000433         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_403351,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000433 DASVLL010000000
VERSION     DASVLL010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403351"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VF402_07170"
     CDS             complement(<1..332)
                     /locus_tag="VF402_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800113.1"
                     /translation="MMAADRTPRLAPMAVLALAAFALLLAMRPAAADEWRGNNAARVN
                     ATIRPCFGMLLDAQLRACGPMRHYREDGYRYGYYGSRADATIDCDRAAPGYVEAVARR
                     IRPYGVLYL"
     gene            complement(364..1806)
                     /locus_tag="VF402_07175"
     CDS             complement(364..1806)
                     /locus_tag="VF402_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7800114.1"
                     /translation="MSEEKPRAQAAVARLRPLIGLALGLFAAVALSGCGRVADVLHHL
                     FGGGPAVSGQYACPRPPDPFQTQGFNGQEREIRYYRHLAFANDFFAQLELANRYAAIN
                     AEDKNLEDPIESATWLVMALSNAQGYLPMNRAIGPDGATMASRYDKCRAYERERAYRM
                     LDQELSMMDSTERDKVRDRVIYIQSTLGADGFRTLARLYDDQYGPMGEPANDPQAVRA
                     MMRGANYGMSNVRTLFERSDVDAYLYNYKASETGDVSAYVLLKDFEKSAPDRQRYAAF
                     VETKARRWTAPYEFYPPDAPGGGVPHSDESRLSDAQSYALTRMDELPFIHVVRALDYL
                     NLLHVACFDAKDVPPQTVSALRSMIGMPAQPGYLTPLDRVRAIELAAVNGDSRSQLVL
                     AVMYSEGVGVGVDYARAFYWYQQAEKQGSPEARYAMSTYFDLGLKGVADQDKAQAVVM
                     QLDAALEGFRPSVNRLQDVLAQVSQTAQQQ"
     gene            2209..2688
                     /locus_tag="VF402_07180"
     CDS             2209..2688
                     /locus_tag="VF402_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7800115.1"
                     /translation="MQGKAAIYKDVTRDVLAVLDGEPDRIARMATCVSLLAQAFPAWF
                     WTGFYIVDAHKKAQGIDELVVGPYMGTLGCLRIRFGRGVCGAAAAARTSQVVPDVHAF
                     ADHIACDSRSNSEIVVPVFDAAGELIAVFDSDSESFGAYDDADRVHLEALMRAVFAV"
     gene            2765..>3552
                     /locus_tag="VF402_07185"
     CDS             2765..>3552
                     /locus_tag="VF402_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7800116.1"
                     /translation="MAQQYIFQMQGLSKSYPGGKKVFENIWLSFYSDAKIGVVGVNGS
                     GKSTLLKIMAGIDKEFSGEARAADGTKMGYLEQEPHLDPDKTVWENVIDWCEEKKIFD
                     RFNEVAALMGEEYSDALMEEMNALQEKIDAGDLWDIDSRIEMAMDALRCPPNDWAVTK
                     LSGGEKRRIALARLLLSKPDMLLLDEPTNHLDAESVAWLQHHLENFPGCVILVTHDRY
                     FLDQVTKWTLELDRGKGVPYEGNYSGWLEQKQKRVVQEQSESEAR"
BASE COUNT          638 a         1133 c         1183 g          598 t
ORIGIN      
        1 agatacaaca ctccgtaggg ccggatgcgg cgcgccaccg cctcgacata gcccggcgcg
       61 gcgcggtcgc aatcgatggt ggcgtcggcg cggctgccgt aatagccgta gcgatagccg
      121 tcctcgcggt aatgccgcat cggcccgcac gcgcgcaact gcgcgtcgag cagcatgccg
      181 aagcacggcc ggatcgtggc gttgacgcgc gcggcgttgt tgccgcgcca ttcgtcggcg
      241 gcggcgggcc gcatcgcaag cagcaacgca aacgccgcca gcgccagcac ggccatcgga
      301 gccagtctcg gggtgcggtc tgcggccatc atgatgcgtt tcatccttgc tattgctgcc
      361 gggctattgc tgctgggccg tctgcgacac ctgcgccagc acgtcctgca aacggttgac
      421 cgaaggccgg aagccttcca gcgcggcgtc gagctgcatg accacggcct gcgccttgtc
      481 ctgatcggcg acgcctttca gccccagatc gaaataggtc gacatggcgt agcgcgcctc
      541 gggcgagcct tgcttctcgg cctgctgata ccagtagaag gcgcgggcgt aatcgacgcc
      601 gacgccgacg ccctcggaat acatgaccgc cagcaccagt tgcgaacgcg aatcgccgtt
      661 gaccgccgcc agctcgatgg cgcgcacccg gtcgagcggc gtcaggtagc cgggctgggc
      721 cggcatgccg atcatcgacc gcagcgccga tacggtctgc ggcggcacgt ccttggcgtc
      781 gaaacaggcg acgtgcagca gattcaggta atcgagcgcg cgcaccacat ggatgaacgg
      841 caattcgtcc atgcgggtca gggcgtagct ctgcgcgtcg ctcaggcggc tttcgtccga
      901 gtgcggcacg ccgccgcccg gcgcgtccgg cggatagaat tcgtaaggcg ccgtccagcg
      961 ccgcgccttg gtctcgacga aggcggcgta gcgctggcgg tcgggcgccg acttctcgaa
     1021 atccttgagc agcacgtagg cgctgacgtc gccggtctcg gacgccttgt aattgtagag
     1081 ataggcgtcg acgtcggagc gctcgaacag ggtgcgcacg ttcgacatgc cgtaattggc
     1141 gccgcgcatc atggcgcgca ccgcctgcgg atcgttggcc ggttcgccca tcggcccgta
     1201 ctggtcgtcg tagagccgcg ccagcgtgcg gaagccgtcg gcgcccagcg tcgactggat
     1261 atagatcacc cggtcgcgca ccttgtcgcg ctcggtcgaa tccatcattg acagttcctg
     1321 atcgagcatg cggtaggcgc gttcgcgctc ataggcgcgg cacttgtcgt agcgcgaggc
     1381 catggtggcg ccgtccggcc cgatggcgcg gttcatcggc aggtagccct gcgcgttgct
     1441 gagcgccatg accagccagg tcgccgattc gatcgggtct tcgagattct tgtcctcggc
     1501 gttgatggcg gcgtagcggt tggccagctc aagctgcgcg aagaagtcgt tggcgaaggc
     1561 gaggtggcgg taatagcgga tttcccgctc ctgaccgttg aagccctgcg tctggaacgg
     1621 atcgggcggg cgcgggcagg cgtattggcc cgacaccgcc gggccgccgc cgaacaggtg
     1681 atgcaggacg tcggcgacgc gcccgcagcc gctcagcgcg acggcggcga acaggcccag
     1741 ggccagcccg atcagcggac gcaggcgcgc cacggcggcc tgcgcgcgcg gcttttcttc
     1801 gctcatgtca cgcctgaaca tcacgccatc gctccggggc ggcaggatta tggttaacgc
     1861 gacacggtgt aagggcgttg ttagaaagga atatgacgat ggtcaacttg cccccacctc
     1921 ttgccggcgc gcagcccctg cgcggtcggc ccctcttgaa gcctctgaaa accttgctga
     1981 aaacgctgca aaacacgccc aacgccgcct gaacgcggcg tttgagataa cgccatcatg
     2041 cgtcaaaagc cgtgccttga ccagagaaaa gtgttaacaa ccccctgccg ccggactttc
     2101 gcgccttacg cgcgttccgg ccacacctta cccctgacgg gcgaaaaacc ggagagcgcc
     2161 gccttgagtc acagcgtgca aaaaccctcg gcgcaaaaac ccctggtcat gcagggcaag
     2221 gccgcgattt acaaggacgt gacaagggac gttctggccg ttctggacgg cgagccggac
     2281 cggatcgcgc gcatggcgac ctgcgtgtcg ctgctggcgc aggcgtttcc cgcctggttc
     2341 tggaccggct tctatatcgt cgacgcgcac aagaaagcgc aggggatcga cgagctggtg
     2401 gtcgggcctt acatggggac gctgggctgc ctgcgcatcc gtttcgggcg cggcgtgtgc
     2461 ggcgcggcgg cggcggcgcg gacctcgcag gtcgtgccgg acgtccacgc cttcgccgat
     2521 cacatcgcct gcgattcgcg ctccaattcc gaaatcgtcg tgccggtgtt cgacgcggcg
     2581 ggcgaactga tcgcggtgtt cgattccgat tcggaaagct tcggcgccta tgacgacgcg
     2641 gaccgcgtcc atctcgaagc gctgatgcgg gccgtgttcg ccgtttaagc cttgagattt
     2701 tacggcgggg cggctaaacg caacgccaat tcccgacatt ccagcaaaag ggcgcaccct
     2761 cctcatggcg caacaatata tcttccagat gcaggggctg agcaagtcct accccggcgg
     2821 caagaaggtc ttcgagaata tctggctctc cttctattcc gacgccaaga tcggcgtggt
     2881 cggggtcaac ggctcgggta agtcgaccct gctgaagatc atggccggca tcgacaagga
     2941 attctccggc gaggccaggg ccgccgacgg caccaagatg ggctatctgg agcaggagcc
     3001 gcatctcgac ccggacaaga cggtctggga aaacgtcatc gactggtgcg aggagaaaaa
     3061 gatcttcgac cgcttcaacg aagtcgccgc cctgatgggc gaggaatatt ccgacgcgct
     3121 gatggaggag atgaacgcgc tgcaggaaaa gatcgacgcg ggcgatctgt gggacatcga
     3181 cagccgcatc gaaatggcga tggacgcgct gcgctgcccg ccgaacgact gggcggtcac
     3241 caagctgtcg ggcggtgaaa agcgccgcat cgcgctggcc cgcctgctgt tgtcgaagcc
     3301 ggacatgctg ctgctggacg aaccgaccaa ccacctcgac gccgagtcgg tggcgtggtt
     3361 gcagcaccat ctggaaaact tccccggctg cgtgatcctg gtcacccacg accgttactt
     3421 cctcgatcag gtgaccaagt ggacgctgga gctggatcgc ggcaagggcg tgccgtacga
     3481 gggcaactat tccggctggc tggagcagaa gcagaagcgc gtggtgcagg agcagtccga
     3541 atccgaagcg cg
//
LOCUS       DASVLL010000434         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_458786,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000434 DASVLL010000000
VERSION     DASVLL010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458786"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1006
                     /locus_tag="VF402_07190"
     CDS             239..1006
                     /locus_tag="VF402_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7800117.1"
                     /translation="MSDHADAVQAQFDPQAAAYLSSRVHAEGPDLKRAAELVAAAIPA
                     DKGKALDVGCGAGHLAYALAPHLSRTVAADPSPAMLATVADAARKRWLNIETRQARAD
                     ALPFADGEFCVAASRYSAHHWQGLEAAVAEMRRVVKTGGYLLMIDVLGHEDDLVDTHM
                     QAIELLRDRSHRRNLKGSQWRRVIAASGFDMIEEESWPLPMAFEPWVARMRTPALRVE
                     AIRAVLDEAPAEVRDALEVQPDYAFAFRTGLFWAKAV"
     gene            1108..>2275
                     /locus_tag="VF402_07195"
     CDS             1108..>2275
                     /locus_tag="VF402_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7800118.1"
                     /translation="MQFQKIKLSGFKSFVDATEFRIDPGLTGIVGPNGCGKSNLLEAL
                     RWVMGATSAKAMRGAGMDDVIFAGSDKRPSRNWAEVTLSIDNADRLAPQPFTDQPVLE
                     VSRRIDRGAGSSYRINGKEVRARDVQLLFADASTGANSPALVRQGQISELIAAKPQNR
                     RRVLEEAGGVSGLHTRRHEAELRLSAAETNLSRLDDIARELDSALSRLKREARQADKY
                     KKISAEIRALQRAILHARWLEARSLLEGAVRDMQDQSGRVETATRAAAAAQTAALKAA
                     EAIAPLREEEAVAATVMHRLNIEKERLDLEEKQAVSEIERLKSEIRRLQEDHQRERDL
                     AADGAQQVERLQASLARVRDEIAAAPSREPELKAAVDAAEARRAAAAAAIETLAA"
BASE COUNT          365 a          800 c          789 g          321 t
ORIGIN      
        1 agggcggcgg cctcctcgcg gcgcaggcct ttggcgcggc ggcggcggcc cgtcggcagg
       61 ccgcaggctt cgggcgtcag gcgctcgcgc gcggcgcgca cgaaggcggc caggttttcg
      121 gcgtcggcga tgtgcggcat agcggctcca gagtgttacg ccttatacca ctataaactc
      181 cagtcttaca cccccttaaa agccgtctat cctaagcccc gttattaagg agacacccat
      241 gtccgaccac gccgacgccg ttcaggccca gttcgatccg caggccgccg cttacctgag
      301 cagccgcgtc cacgccgagg ggcccgacct gaagcgcgcc gccgagctgg tcgccgccgc
      361 cattccggcg gataagggca aggcgctcga cgtcggctgc ggcgccgggc atctggccta
      421 cgcgctggcg ccgcatctga gccggaccgt ggccgccgat ccgtcgcccg ccatgctggc
      481 gaccgtggcc gacgccgcgc gcaagcgctg gctgaacatc gagacgcggc aggcgcgcgc
      541 cgacgccctg cccttcgccg acggcgagtt ctgcgtcgcc gcctcgcgct attcggcgca
      601 ccactggcag gggctggagg cggcggtcgc cgagatgcgc cgcgtggtca agaccggcgg
      661 ctatctgctg atgatcgacg tgctggggca tgaggacgat ctggtcgaca cgcacatgca
      721 ggcgatcgag ctgctgcgcg accgctcgca ccgccgcaat ctcaaggggt cgcaatggcg
      781 ccgggtcatc gccgcgtccg gcttcgatat gatcgaggag gaatcgtggc cgctgccgat
      841 ggcgttcgag ccgtgggtgg cgcggatgcg cacgcccgcc ttgcgcgtcg aggcgatccg
      901 cgccgtgctg gacgaagccc ccgcggaggt gcgcgacgct ctggaggtgc agccggatta
      961 cgccttcgcc ttccgcaccg gcctgttctg ggcgaaggcg gtgtaagcga tccacaggaa
     1021 tcgccggggc gggcgcgaag tttcttgacg caaccgcgca tggtatgagt gttaaagaat
     1081 cgtttaacct cccgccccgg ccctgtcttg cagttccaga agatcaagct ttcgggattc
     1141 aagtccttcg tcgatgcgac cgaattccgc atcgatccgg ggctgaccgg cattgtcggg
     1201 ccgaacggct gcggcaagtc gaacctgctg gaggcgctgc gctgggtgat gggcgcgacc
     1261 tcggccaagg cgatgcgcgg cgccggcatg gacgacgtga tcttcgccgg ttcggacaag
     1321 cgcccgtcgc gcaactgggc cgaggtgacg ctgagcatcg acaacgccga ccggctggcg
     1381 ccccagccct tcaccgacca gccggtgctg gaggtgtcgc gccgcatcga ccgcggcgcg
     1441 gggtccagct atcgcatcaa cggcaaggag gtgcgcgcgc gcgacgtgca gcttctgttc
     1501 gccgacgcct cgaccggcgc caattcgccc gccctcgtcc gtcagggcca gatttccgaa
     1561 ctgatcgccg ccaagccgca gaaccgccgc cgcgtgctgg aggaagcggg cggcgtgtcg
     1621 ggcctgcaca cccgccgcca cgaggccgaa ctgcgcttat cggccgccga aaccaacctg
     1681 tcgcgcctcg acgacatcgc gcgcgaactc gattcggccc tgtcgcggct caagcgcgag
     1741 gcgcgtcagg cggataagta caagaagatt tccgccgaga tccgcgcgct gcaaagggcc
     1801 atcctgcacg ccagatggct ggaggcgcgc agccttctgg aaggcgccgt gcgcgacatg
     1861 caggaccagt cgggccgcgt cgaaaccgcg acccgcgccg ccgccgccgc ccagaccgcc
     1921 gcgctgaaag ccgccgaggc catcgcgccc ttgcgcgagg aggaggccgt cgccgccacg
     1981 gtcatgcacc ggctgaacat cgaaaaagag cgcctcgacc ttgaggagaa gcaggcggtc
     2041 agcgaaatcg agcgcctgaa aagcgagatc cggcgcctgc aggaggatca tcagcgcgaa
     2101 cgcgacctcg ccgctgacgg cgcgcagcag gtcgagcgtt tgcaggcgtc gctggcgcgg
     2161 gtgcgcgacg agatcgccgc cgcgccgtcg cgcgaaccgg agttgaaagc cgccgtcgac
     2221 gccgccgagg cgcgccgcgc cgccgccgcc gccgccatcg aaaccctggc cgccg
//
LOCUS       DASVLL010000435         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_458798,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000435 DASVLL010000000
VERSION     DASVLL010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458798"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VF402_07200"
     CDS             complement(<1..1194)
                     /locus_tag="VF402_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7800119.1"
                     /translation="MRPSVYVLAFALLSGAGPALADGADAPAPGAQVVGRQESPDHVL
                     HVDVTLDGEGRLAYAVTRNGHEVIAPSRLGFVLQNAPKLDGRFTLDQVATDSHDDTWT
                     QPWGEWSAIRDHYNELRVTVAQAPYANRKMTVVFRVFDDGVGFRYEFPQQASLPVVKI
                     GEELTEFDVADPATAWWAPAGELSNAEYLYEKTPLAEVGQAQTPLTVKTDRGVYIAFH
                     EAALVDYAAMWIRRIDGQRLRAQLAPSPLGPSVVRAAPFTTPWRTMIIADSAPGLYMS
                     HIELNLNAPNKLGDVSWVRPYKYVGVWWEMHLDHSTWAQGPRHGATTQNAEKYIDFAA
                     KNGFRGVLVEGWNKGWDGVWYGDGSDFSFTEATPDFDLKAVADYGLKKGVHLIGHHET
                     GGNIAH"
     gene            complement(1318..2361)
                     /locus_tag="VF402_07205"
     CDS             complement(1318..2361)
                     /locus_tag="VF402_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7800120.1"
                     /translation="MTVKRPTSFDIAALTGVSQTTVSRALAESPLVSHETRRKVQDAA
                     RALRYKVDANARKLRSRKIHTLAILISEDLEKNDNPINPFFLPLLGSILRYAGQKGYD
                     VLVSLQQPADDWGTDYGFSRRADGVIFLGCKDYQSYTHKFEYLNEVGDPWVVWGVDRP
                     DCARLCIGSDNEGGAYNAVTHLANLGRRRIAYLGKFQPEHPEFKERYQGYRRALQDAG
                     LPYDENLRRDVFLDREEGVAAVAQMFAGGHAFDAVFCATDMLAMGAMQELIRRGLRVP
                     EDVSVMGFDDLWVCSSLSPSLSTVRQDTVAAAQALVDGLSNLIEDEPAPDTRIPTQLV
                     IRESCGGLNLRRH"
BASE COUNT          408 a          843 c          750 g          410 t
ORIGIN      
        1 atgggcgata ttgccgccgg tttcgtggtg gccgatcaga tgcacgccct ttttcagccc
       61 atagtccgcc accgccttga ggtcgaagtc cggcgtcgcc tcggtgaagg agaagtccga
      121 gccgtcgccg taccacacgc cgtcccagcc cttgttccag ccctcgacca gcacgccgcg
      181 aaagccgttc ttcgccgcga aatcgatgta tttttcggcg ttctgcgtcg tcgccccgtg
      241 ccgcggcccc tgcgcccagg tcgaatggtc gaggtgcatc tcccaccaca cgccgacata
      301 tttgtacggc ctgacccacg acacgtcgcc cagcttgttc ggtgcgttca ggttcaactc
      361 gatatgcgac atgtaaaggc ccggcgcgct atcggcgatg atcatcgtcc gccacggcgt
      421 cgtaaacggc gcggcgcgca cgaccgaagg ccccagcggc gacggcgcca gttgcgcgcg
      481 caggcgctgg ccgtcgatgc ggcgaatcca catggcggcg taatcgacca gcgccgcctc
      541 gtgaaaggcg atatagacgc cacggtcggt cttgacggtc agcggcgtct gcgcctgacc
      601 gacctcggcc agcggcgttt tttcgtacag atattcggcg ttggacaact cgcccgccgg
      661 cgcccaccac gcggtcgccg gatcggcgac gtcgaactcg gtcagctcct cgccgatctt
      721 caccaccggc agcgacgcct gctgcggaaa ctcgtagcgg aagccgaccc cgtcgtcgaa
      781 gacgcggaac accaccgtca tcttgcggtt ggcatagggc gcctgcgcga cggtgacgcg
      841 cagttcgtta taatggtcgc ggatggcgct ccattcgccc cacggctgcg tccatgtgtc
      901 gtcgtggctg tccgtcgcca cctgatcgag cgtgaagcgc ccgtccagct tgggcgcgtt
      961 ctgcaacacg aagccgagcc gcgacggcgc gatgacctca tgcccgttgc gcgtcaccgc
     1021 gtaggccagc cgtccctcgc cgtccagcgt gacgtcgacg tgcaggacat ggtccggcga
     1081 ctcctgccgc cccaccacct gcgcgccggg cgccggcgcg tcggcgccgt ccgccagagc
     1141 cggacccgcg ccggacagca gcgcgaaggc gagaacatag acggatggac gcatgacgat
     1201 gtttcccctg tttcggcgcc ccggcgggct tgtcgctcca tcattaaatg atgatcatca
     1261 tattttaaag cgtaaaaact cccggcccgg ctatgggccg cggccatgag tacgtattca
     1321 atgccgccgt aaattcagcc cgccgcacga ttcgcggatc accagttgcg tcggaatgcg
     1381 cgtgtccggc gccggttcgt cctcgatcag gttgctcagc ccgtccacca gagcctgcgc
     1441 ggcggcgacc gtgtcctgcc gcaccgtgct cagcgacggc gacagcgacg aacagaccca
     1501 caggtcgtcg aagcccatga ccgacacgtc ctccggcacg cgcagcccgc gccggatcag
     1561 ctcctgcatc gcgcccatgg ccagcatgtc ggtggcgcag aacaccgcgt cgaacgcgtg
     1621 gccgcccgcg aacatctgcg ccacggcggc gacgccctcc tcgcggtcga gaaacacgtc
     1681 gcggcgcagg ttttcgtcgt agggcagccc cgcgtcctga agcgcgcggc gatagccctg
     1741 ataccgctcc ttgaactcag gatgctccgg ctggaacttg ccgagatagg cgatgcgccg
     1801 ccgccccaga ttggccaggt gcgtgacggc gttataggcg ccgccctcat tgtccgagcc
     1861 gatgcacaga cgcgcgcagt cgggccggtc cacgccccag accacccacg gatcgccgac
     1921 ctcgttcaga tactcgaact tgtgcgtata ggactgatag tccttgcagc cgagaaagat
     1981 caccccgtcg gcgcggcgcg aaaagccgta atccgtcccc cagtcgtcgg cgggctgttg
     2041 cagcgacacc agcacgtcat agcccttctg gcccgcatag cgcaagatcg agccgagcag
     2101 cggcaggaag aacggattga tcggattgtc gttcttctcc agatcctcgg agatcaggat
     2161 ggccagggtg tgaatcttgc gcgaccgcag cttgcgcgca ttggcgtcga ccttgtagcg
     2221 cagggcgcgc gcggcgtcct gcaccttgcg gcgcgtctca tggctgacca gcggcgattc
     2281 ggccagcgcg cgcgacacgg tggtctgcga cacgccggtc aaggcggcga tatcgaacga
     2341 ggtcggacgt ttgaccgtca tgacggctcc cctcctgaca aaccgggcgc atggcccgcg
     2401 aacgtttccc t
//
LOCUS       DASVLL010000436         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_403480,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000436 DASVLL010000000
VERSION     DASVLL010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403480"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VF402_07210"
     CDS             <1..144
                     /locus_tag="VF402_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX7800121.1"
                     /translation="MCPNIPDDMKELYVQKGAGHYGVFNGRRFRDEIYPRIRDFIAKH
                     DVV"
     gene            159..896
                     /locus_tag="VF402_07215"
     CDS             159..896
                     /locus_tag="VF402_07215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX7800122.1"
                     /translation="MTRLFSPREAYADGQVIDCGDYALRLKVNRRARRISLRIDNKTG
                     EAVASAPSPRHLKDAVAFARARHDWIVGMRRREPERAPFAPGLRLEIRGHPVTLAEAK
                     GVVTPRAEADEDGGWRLVAGGDPSLYARRIERYLRAQALKAFQAETDAFAARLGVSGV
                     RVSLFDARARWGSCTPGRKAIRYSWRVILAPREVFSYLAAHEVAHLKHPDHSPAFWAC
                     VSELYGRDWKPARKWLKEQGHSLFRFS"
     gene            953..1531
                     /locus_tag="VF402_07220"
     CDS             953..1531
                     /locus_tag="VF402_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800123.1"
                     /translation="MSDKTLFGCMAVVLMLGLASAPGAQPVNSVFGPMRPQGTGAAAR
                     PLPPRLKVFLQASYRDSQATPATEVDIISAPLHTAHGELILVYATGGYVCGTGGCNLL
                     VLKPTATGYSLIGDVQTWAPIRLLPTSHFGRPDIAVWVQGGGVRPGYAAAMRYDGETY
                     PRDDGVYERHRLGKVSGRILISGREKGVHLFP"
     gene            complement(1561..>2362)
                     /locus_tag="VF402_07225"
     CDS             complement(1561..>2362)
                     /locus_tag="VF402_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7800124.1"
                     /translation="ANDVGRDKVAEAAHRLGITSTINTDPAMALGTSEVAPIEMAQAY
                     IPFSNGGYRAAAHGIIRIRTASGKVLYQYRDPSQDGGRVRVINNPPLMEMNQMLRAVM
                     HGGTGTGAYIDGYDLAGKTGTTSDFRDAWFDGYTGGFVTVVWVGRDDNTVMRGVTGGS
                     TPAAIWKTYMTSALKRIQVSPIPDGPPAAGTLSPVAEDALRDLLGSQADAVAASQAAN
                     DNPQPAEDGLPPVVTMKPIPNPDDKKPAANKDDPSLDDIFSQAQKKNQ"
BASE COUNT          342 a          783 c          824 g          413 t
ORIGIN      
        1 atgtgcccga acattcccga cgacatgaag gaactctacg tgcagaaggg cgccgggcat
       61 tacggcgtgt tcaacggccg ccgcttccgc gacgaaatct atccgcgcat ccgcgacttc
      121 atcgccaagc acgatgtggt gtgatggcgg acggccacat gacccgcctg ttctcgccgc
      181 gtgaggctta cgccgacggt caggtgatcg actgcggcga ttacgccctg cggctgaagg
      241 tcaaccgccg cgcccgccgc atctccttgc gcatcgacaa caagaccggc gaggcggtcg
      301 cctcggcgcc gtcgccgcgc catctgaagg acgcggtcgc cttcgcgcgc gcgcgacatg
      361 actggatcgt cgggatgcgc cgccgcgaac cggagcgcgc gccgttcgcg ccgggcctga
      421 ggctggagat tcgcggccat ccggtcacgc tggccgaggc gaagggcgtg gtcacgccgc
      481 gcgccgaagc ggacgaggac ggcggctggc ggctggtggc gggcggtgat ccatcgctgt
      541 acgcccgccg gatcgagcgt tacctgcgcg cgcaggcgct caaggcgttt caggccgaaa
      601 ccgacgcctt cgcggccagg ctgggcgtgt cgggcgtcag ggtctccctg ttcgacgccc
      661 gggcgcgctg gggctcctgc acgccggggc gcaaggcgat ccgctattcg tggcgcgtga
      721 tcctggcccc gcgcgaggtg ttcagctatc tggccgcgca cgaggtcgcg cacctgaaac
      781 accccgacca cagccccgcc ttctgggcct gcgtgagcga actctacggc cgcgactgga
      841 aaccggcgcg caaatggctg aaagagcagg gccacagcct gttccggttt tcctgaatcc
      901 ggccttccca gtctggtgaa tttatgatgt cctcacgccg cgggagggga tcatgtctga
      961 caagacctta ttcggctgca tggccgtcgt tctcatgctg gggcttgcgt ccgcgcccgg
     1021 cgcgcagccg gtcaacagcg tcttcggccc gatgcggccg caaggaaccg gcgcggcggc
     1081 gcgtccgttg ccgccgcgcc tcaaggtgtt tctacaggcc agctatcgcg acagtcaggc
     1141 gactccggcg acagaggtcg atattatcag cgcgccgctt cataccgcgc acggcgaact
     1201 gatattggtt tacgccaccg gcggatatgt ttgcggcaca ggcggttgca acctgttggt
     1261 cctgaagccg acggcgacgg gctatagcct catcggcgat gtccagacat gggcgccgat
     1321 ccgcctgctg ccgacgtcgc attttggccg ccccgacatc gccgtgtggg tgcagggcgg
     1381 cggcgtcagg cccggctatg cggccgcgat gcgttacgac ggcgagacct atcctcgcga
     1441 cgacggcgtt tatgagcgac atcgtctggg caaggtcagc ggccgtattc tgatcagcgg
     1501 ccgggaaaaa ggcgttcacc tctttccctg accctgatgg atgcagggcg gtcgcccccg
     1561 ctactgattc ttcttctggg cctgcgagaa gatgtcgtcc agcgacgggt cgtccttgtt
     1621 ggccgccggt ttcttgtcgt cggggttcgg gatcggcttc atggtgacga cgggcggcag
     1681 gccgtcctcg gccggttgcg ggttgtcgtt ggccgcctgc gaggccgcca cggcgtcggc
     1741 ctgcgagccg agcaggtcgc gcagcgcgtc ctcggccacc ggcgacaggg ttcccgccgc
     1801 gggcgggccg tccgggatcg gcgagacctg gatgcgtttc agcgccgagg tcatgtaggt
     1861 cttccagatc gccgccgggg tcgaaccgcc ggtcacgccg cgcatgacgg tgttgtcgtc
     1921 gcgcccgacc cagaccacgg tgacgaagcc gccggtgtag ccgtcgaacc acgcgtcgcg
     1981 gaagtcgctg gtcgtgccgg tcttgccggc cagatcgtag ccgtcgatat aggcgcccgt
     2041 gccggtgccg ccgtgcatga cggcgcgcag catctggttc atctccatca gcggcggatt
     2101 gttgatcacg cgcacgcggc cgccgtcctg cgacgggtcg cggtactgat acagcacctt
     2161 gcccgaggcg gtgcggatgc ggatgatgcc gtgcgcggcg gcgcggtagc cgccgttgga
     2221 gaacggaata taggcctgcg ccatctcgat gggcgccact tccgaggtgc ccagcgccat
     2281 ggccggatcg gtgttgatcg tggaggtgat ccccagacgg tgcgcggcct cggccacctt
     2341 gtcgcgcccg acgtcgttgg cc
//
LOCUS       DASVLL010000437         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_242604,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000437 DASVLL010000000
VERSION     DASVLL010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242604"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="nuoF"
                     /locus_tag="VF402_07230"
     CDS             complement(<1..823)
                     /gene="nuoF"
                     /locus_tag="VF402_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7800125.1"
                     /translation="MVGILEDKDRIFTNLYGFHDWGLEGAKQRGAWNATRDILSYGRD
                     WIITNVKNSGLRGRGGAGFSTGLKWSFMPKEVKDRPHYLVVNADESEPGTCKDREIMR
                     HDPHLLIEGCLIASFAMQAHACYIYLRGEYVQERERMEAAVKQAYEAKLIGKNNIHGW
                     DFDVYIHHGAGAYICGEETALLESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIA
                     VVGTILRRGAAWFASFGAPNNAGTKLFCVSGHVNKPCNIEEAMSIPFRQLIEDHCG"
     gene            complement(823..1491)
                     /gene="nuoE"
                     /locus_tag="VF402_07235"
     CDS             complement(823..1491)
                     /gene="nuoE"
                     /locus_tag="VF402_07235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7800126.1"
                     /translation="MSLRRLAKEQPASFTFSAETLEKANWWIAKYPENRRRSAVIPIL
                     WLVQKQEGWVSEPAITAIARLLDMPQIRVFEVATFYTMFQLEPVGSAALIQVCGTTPC
                     MLRGSDALMQVCKDKIGPKDKLSKDGKFTWQEVECLGACCNAPMAQINDYYYEDLTPE
                     NLARIIDDFAAGQSPKPGPYNGRHTSEPEGGVTSLTDPRLYDGSAAKKIKIPNAPGTE
                     EVKA"
     gene            complement(1586..1993)
                     /locus_tag="VF402_07240"
     CDS             complement(1586..1993)
                     /locus_tag="VF402_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800127.1"
                     /translation="MNGSAAFAIWMRSHMVLSTALIYAFVALVIAALILGRWYFSRSA
                     WPFHPGGSTGFLRDEFLRLGVIFIPYAIVMLIYRFFVYDLHPELATSPITWVLLLSVI
                     VFRRASRFIPFVRDAGRRIDAARKMAREATFSA"
     gene            complement(1990..>2251)
                     /locus_tag="VF402_07245"
     CDS             complement(1990..>2251)
                     /locus_tag="VF402_07245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517402.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX7800128.1"
                     /translation="LIHHFKLYTEGFHTPEGEVYACVEAPKGEFGVYLVSDGTNRPYR
                     CKIRAPGFPHLQAMDWMNRGHLLADVSAILGSLDIVFGEIDR"
BASE COUNT          422 a          702 c          710 g          417 t
ORIGIN      
        1 cgccgcagtg atcctcgatc aactggcgga acgggatcga catggcttct tcgatattgc
       61 acggcttgtt gacgtggccg gaaacgcaga acagcttggt gcccgcattg ttcggcgcgc
      121 cgaacgaggc gaaccatgcc gcgccgcgcc gcaggattgt gccgaccacc gcgatcgatt
      181 cgacgttgtt caccgtggtc gggcaaccgt agaggcccgc gcccgccggg aagggcggct
      241 tcaggcgcgg ctggcccttc ttgccttcca gcgattccag cagggctgtt tcctcgccgc
      301 agatataggc gccggcgccg tggtggatat agacgtcgaa atcccagccg tggatgttgt
      361 tcttgccgat cagcttggcc tcgtaagcct gcttgacggc ggcctccatg cgctcgcgct
      421 cctgcacgta ctcgccgcgc aggtagatgt agcaggcgtg cgcctgcatg gcgaacgagg
      481 cgatcaggca gccttcgatc agcagatgcg gatcgtggcg catgatttcg cggtctttgc
      541 aggtgccggg ttcggattcg tcggcgttga ccacgaggta gtgcgggcgg tccttgacct
      601 ccttgggcat gaaggaccac ttcagaccgg tcgagaagcc ggcgccgccg cgtccgcgca
      661 ggccggaatt cttcacattg gtgatgatcc agtcgcgccc gtaggacagg atgtccctgg
      721 tggcgttcca cgcgccgcgc tgcttcgcgc cctccaggcc ccagtcgtgg aagccgtaga
      781 ggttggtgaa aatccggtcc ttgtcttcga gaatcccgac cattacgctt tcacctcttc
      841 tgtacccggc gcattgggaa tcttgatctt cttcgccgcc gagccgtcat agagcctggg
      901 gtcggtcagc gaggtgacgc cgccttccgg ctcggaggta tggcggccgt tgtacggtcc
      961 cggcttgggc gactggcccg ccgcgaagtc gtcgatgatc cgggccaggt tttccggcgt
     1021 caggtcttcg taataatagt cgttgatctg cgccatcggg gcgttgcagc acgcgcccag
     1081 acactcgacc tcctgccagg tgaacttgcc gtccttcgac agcttgtcct tggggccgat
     1141 cttgtccttg cacacctgca tcagcgcgtc ggaaccgcgc agcatgcacg gcgtcgtgcc
     1201 gcacacctgg atcagcgccg ccgagccgac cggctcaagc tggaacatgg tgtagaaggt
     1261 cgcgacctcg aagacgcgaa tctgcggcat gtccagcaat ctggcgatcg cggtgatcgc
     1321 cggttcggac acccagccct cctgcttctg caccagccac aggatcggaa tcaccgcgct
     1381 gcggcggcgg ttctcaggat atttggcaat ccaccagttg gccttttcca gcgtttccgc
     1441 cgaaaaggtg aagctggccg gttgctcctt ggcgagacga cgtagggaca tcaggcctct
     1501 ctcataagcg aacaggacga gttgcgggtg gctttacacc agtgcgccgc aaaagacagg
     1561 gtaaaatcgg aattgatcac ggctttcatg cggaaaacgt cgcctcccgc gccattttac
     1621 gcgccgcgtc gatacgccgc ccggcgtcgc gcacgaacgg aataaaccgc gaggcgcgcc
     1681 ggaacacgat caccgacagc agcagcaccc aggtgatcgg cgaggtcgcc agttccggat
     1741 gcaggtcata aacgaaaaaa cggtagatca gcatgacgat cgcgtaagga atgaagatca
     1801 cgcccagacg caggaattcg tccctgagga agcccgtcga gccgccggga tgaaacggcc
     1861 aggcgctgcg gctgaaatac cagcggccaa ggatcagcgc ggcgatgacc agcgccacga
     1921 aagcgtagat cagcgccgtg gacagcacca tatggctgcg catccagatc gcgaaggccg
     1981 ccgaaccgtt catcggtcga tttccccgaa cacgatgtcg agcgagccga ggatggccga
     2041 gacgtccgcc agcaggtggc cgcggttcat ccagtccatc gcctgcaaat gcgggaagcc
     2101 cggcgcgcga atcttgcagc ggtaaggacg attcgtgccg tcggacacca gatagacgcc
     2161 gaactcgccc ttcggcgcct cgacgcaggc gtaaacctcg ccctccggcg tatggaagcc
     2221 ttcggtgtag agtttgaagt gatggatcag c
//
LOCUS       DASVLL010000438        10158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_28400, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000438 DASVLL010000000
VERSION     DASVLL010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28400"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2283
                     /locus_tag="VF402_07250"
     CDS             <1..2283
                     /locus_tag="VF402_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence domain protein"
                     /protein_id="HEX7800129.1"
                     /translation="KTLIKGATRAGKPAVGAVTLRALAQDAASFEDKPDLKQTAYDGA
                     VETAVLEQSGPVRSVIRLEGRHTDGSRQWLPFTVRLYFYAGSDAVRIVHSFIFDGDET
                     KDFIKGLGVVAQAPMSDELYNRHIRFSGQNDGVWGEAVRSLTGLRRDPGKAAREAQIA
                     GKRVDPAMIAPAVGDNLPNSPYWNDFTCAQATPDGYTIRKRVEPGHAFIESAADHRAS
                     GLCYAGSPQGGVALGLKDFWQRAPTRLDIRGAAGDMAELTAWLWSPDAPAMDIRPYRP
                     DWGMDTYAKQNAGLLLTYEDFEPGWNRPYGIARTSELTLWALPATPERQRFADMAAAL
                     SQTPRLTVAPERLHLVEGIFGAWSLPDSSTPTRALIEGRIKYQLDYYLQQIDERKWYG
                     FWDYGDVMHTYDADRHVWRYDIGGFAWDNSELQSDMMFWYGYLRSGRADMFRMAEAMT
                     RHTSEVDVLHIGPYKGFGTRHGVQHWADSSKQPRVSNAAFKRFYYYLTADERVGDLMR
                     DLVTSDQTLKDVYIERKVASAGGSGIGIRKHVEGTVDCSFGTSWGSFIAAWLTEWERT
                     QDTRWRDRILTGMNSIAKLKYGWFCGGAPYDLKTGKFVGPGDQVSVSHLNAVFGVVEI
                     TEELLTLIDAPAYKKVWLDYCIYYNSSPEEIKAFLGVVPKDRSLRDAHSRLTAYAARE
                     LKDPKLAARAWSEFFDGDNRRFRTRLNPQEDKVTGPDVLYAIEEDPAISTNGASQWGL
                     AAIENLQLIGDSLEANAPKG"
     gene            2413..3180
                     /locus_tag="VF402_07255"
     CDS             2413..3180
                     /locus_tag="VF402_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7800130.1"
                     /translation="MTHHRRNVAITGADSGIGAACARAFGALGDHVTVFYYHDKDGAE
                     ASVRAVEAAGGQAVAIQCAVNDRDSVDRAFADMEARYGPARVFVNSAGVNMRGVRVRD
                     MAPEVWLRTVATDLTGAYLTSKRFLQSCPRGPASIVHISSIHAEAVRDGGADYCAAKA
                     GLNNLVRTLAIEEAANAIRVNAIAPGMILTPMNQRAVDDPDYRRKLERHIPMGRAGRA
                     EEVAHLARWLASDEAAYITGASLVIDGGLSLLQAVGA"
     gene            3177..4148
                     /locus_tag="VF402_07260"
     CDS             3177..4148
                     /locus_tag="VF402_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800131.1"
                     /translation="MTRQAARSAPDPVTGDRKEHVVTGRGGVDFNVEALQDVRLNGPR
                     ALCERDLLSPYVWRAPDGRLGLMVRAAPPAGKPRTDTGQIWAGWSHDGVTFDMLDAPV
                     LTGGPEDTDIGGVEDPTVLLREDGSYVVYYTGVGPDMTHGEMFYATGPSVDRLSKTGV
                     ALASTKSMGNTKEATVGRTADGRWRLFYEYAAQNASRVGLAVGPDVGGPWHERPTPFM
                     PREDGWDNWHLSPGPMLMDRPGEPVMFYNGATRDARWRIGWIAFDRDCRTVVDRCIEP
                     LLTPPPVEDRTATDIAFAASVIVQDGEIWLYYSLEDRRLARARLRQS"
     gene            4166..4531
                     /locus_tag="VF402_07265"
     CDS             4166..4531
                     /locus_tag="VF402_07265"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HEX7800132.1"
                     /translation="MHGHKRWTIPEGYLPPDDPAKPRPLVSHEAFCVLNPGDDDADIE
                     VTIYYGDREPVGPYRMRVAARRTVHFRFNDLNDPEPIPAGVDYASVITASAPILVQHT
                     RLDSRSGTIALMTTMAIAG"
     gene            4538..5767
                     /locus_tag="VF402_07270"
     CDS             4538..5767
                     /locus_tag="VF402_07270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX7800133.1"
                     /translation="MTAFRERGAAAQMDDRPLRSYYTGANPALAVDIDDLRARAHRLT
                     PRFVLEYLEGGAGQETTLARERAVYGEWLFMPRTLMDEARRDLTRPLLGRDAKMPLLV
                     GPTGLNGLFRRGADLELARACARFGLPFVQSTMSNERMETVAGVEGLRHWWQLYVFGP
                     DEIWQSLVDRAAACGCEALVVTTNSQVYGQRQWSTRTRSEQALPPPAVLFDMLRHPGW
                     LLATMARGMPEFSNVVDYIPKAHRGFFDSAYWIRGQMPAALSWRDIARIRARWKGPMF
                     VKGVLNLEDLDPALDSGVDGVMMGSHGGRQADGAVSALDLLGRAKDRVGDRLALYVSG
                     GLRHGADLLKALGLGADAVIAGRAPLYGLCAYGEAGVYRALEILRDEALNEMAQMGAP
                     SPDQLGRLLIRRDRLPL"
     gene            complement(5768..6796)
                     /locus_tag="VF402_07275"
     CDS             complement(5768..6796)
                     /locus_tag="VF402_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX7800134.1"
                     /translation="MTLSIHNITRTFSGFAALKDISFDVQPGEFIALLGPSGSGKTTL
                     LRLLAGLDAPDSGAITFQGEDYLKLDPRQRRVGMVFQSYALFRHMTVAQNIAFGLDVR
                     SGKAKPPKAEIRATVERLLKLVQLDGLGKRYPSQLSGGQRQRVALARALAIEPRMLLL
                     DEPFGALDALVRKDLRRWLRHIHNETGVTTIFVTHDQEEALDLADRVVVLKDGRIEQI
                     GTPLDIFDAPASAFVFDFLGAANRLPCRVRDGRALFDGFDAPLAAPVADGDYVARFRP
                     FEADLSREGPGLPVTVSAVLAAGGTLRLELRGDDGALFETEQSRAADLAYVRDAALRL
                     RPQKVFVY"
     gene            complement(6796..7641)
                     /gene="cysW"
                     /locus_tag="VF402_07280"
     CDS             complement(6796..7641)
                     /gene="cysW"
                     /locus_tag="VF402_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX7800135.1"
                     /translation="MTARAAKPPKTAGAIALLGFTLIWLGVVIVLPLVYVLATAFAKG
                     VAPWLSALGNEDTIDAIKLTLLVAVLSVPLNAGFGLCAAWAITRFEFPGKSLLLAVLD
                     LPFSLSPVIAGLVWILLFGAQGWFGFLMRDHDVKIVFALPGLVLATVLVTLPFVAREL
                     IPLMEDQGTDEEVAARTLGARGWDIFFRVTLPNIKWALTYGVLLCTARAMGEFGAVSV
                     VSGHIRGLTDTLPLQIEIAYNQYDTVGAFAAASILAGLALVTLILKTLLEWRFAGELA
                     ANRRH"
     gene            complement(7638..8465)
                     /gene="cysT"
                     /locus_tag="VF402_07285"
     CDS             complement(7638..8465)
                     /gene="cysT"
                     /locus_tag="VF402_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX7800136.1"
                     /translation="MHVRTRVLPGFRITLGLTVFYLSVMVVIPLIALIARPWEHGLTG
                     VMFVLTDPRVLHALWISFSIALAAAFVNVFAGLAVAWSLTRYSFPGRRLLDAFIDLPF
                     ALPTAVAGIALSSLYAGNGWLGQALNAVGLKVAYTPLGIFVALVFIGLPFIVRSVQPV
                     LAEFEAEIEEAAQTLGANGWQSLTRIVLPALMPSLVTGFSLALARGVGEYGSVIFIAG
                     NMPYVSEIAPLLIVIKLQEFDYAGAAAVGVVMLALSLLILVLLNLIQRRFARWGAAA"
     gene            complement(8741..9763)
                     /locus_tag="VF402_07290"
     CDS             complement(8741..9763)
                     /locus_tag="VF402_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7800137.1"
                     /translation="MQRRSFLKTLAGGAALAAASGALPGVTLAAGKTRELLNVSYDPT
                     RELYAQYNPLFIQHWKQKTGEDITVRQSHGGSGGQARSVIDGAPADVVTLALAADIDA
                     ISQKAGLLPANWQSRFPNYSTPYTSTIVFLVRKGNPKKIKDWDDLIRLDVSVVTPNPK
                     TSGGARWNYLAAYAYAKKRWGTDARAREFLKNLFNRVPVLDTGARGSTVTFAERGVGD
                     VLIAWENEAFLALNDIGKGKFEIVVPSISILAEPPVAVVDKNALKNGNLAAAREYLSY
                     LYSPEAQEVIAKNYYRPIDKTVAAKYASQFPALNLVTIRDFGGWKKVQAEQFDYGGVF
                     DQITRK"
     gene            complement(9799..>10158)
                     /locus_tag="VF402_07295"
     CDS             complement(9799..>10158)
                     /locus_tag="VF402_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7800138.1"
                     /translation="NTALDVSGSWVLTGESVGYKGGVKPAHDFSLKNGGWGAWELTAR
                     YGYNDIDKDAFTGGFASIGSSARKATDTGLGLGWYWSENLKLLLDYDQTKFEGGAAGG
                     ADRRTEDFTAARVQFRY"
BASE COUNT         1677 a         3366 c         3574 g         1541 t
ORIGIN      
        1 aagacgctca tcaagggcgc gacccgcgcg ggcaagcccg ccgtcggcgc ggtgacgctg
       61 cgggcgctgg cgcaggacgc cgccagcttc gaggacaagc cggacctgaa acagaccgct
      121 tacgacggcg cggtcgagac ggcggttctg gaacagtccg gcccggtgcg cagcgtgatc
      181 cgtctcgaag gccgccacac cgacggctcg cgtcagtggc tgcccttcac ggtgcggctc
      241 tatttctacg ccgggtccga cgcggtgcgc atcgtccaca gcttcatctt cgacggcgac
      301 gaaaccaagg acttcatcaa gggcctcggc gtcgtcgcgc aggcgccgat gtcggacgag
      361 ctttacaacc gccatatccg cttttccggc cagaacgacg gcgtgtgggg cgaggcggtg
      421 cgctcgctga ccggcctgcg ccgcgatccg ggcaaggcgg cgcgcgaagc gcagatcgcg
      481 gggaagcgcg tcgatccggc catgatcgcc ccggcggtcg gcgacaacct gcccaactcg
      541 ccctactgga acgactttac ctgcgcgcag gcgacgccgg acggttacac gatccgcaag
      601 cgcgtcgagc cgggccacgc cttcatcgaa tccgccgccg accaccgcgc gtcgggcctg
      661 tgttacgcgg gcagcccgca gggcggggtg gcgctgggcc tcaaggactt ctggcagcgc
      721 gcgccgacgc ggctcgatat ccgcggcgcg gcgggcgaca tggccgagct gaccgcgtgg
      781 ctgtggtcgc cggacgcgcc cgccatggac atcaggccct atcgtcccga ctggggcatg
      841 gacacctacg ccaagcagaa tgcgggcctg ctgctcacct atgaggattt cgagccgggc
      901 tggaaccggc cctacggcat cgcccgcacc agcgagctga cgctgtgggc gctgcccgcc
      961 acgccggagc gccagcgttt cgccgatatg gccgccgccc tgtcgcagac gccgcgcctg
     1021 acggtcgcgc cggagcggct gcatctggtc gaggggattt tcggcgcctg gagcctgccc
     1081 gattcctcga ccccgacgcg cgccctgatc gagggccgga tcaagtatca gctcgactat
     1141 tacctgcaac agatcgacga gcgcaaatgg tacggcttct gggactatgg cgacgtcatg
     1201 cacacctacg acgccgaccg gcacgtctgg cgctacgata tcggcgggtt cgcgtgggac
     1261 aattcggaac tgcagtcgga catgatgttc tggtacggct atctgcggtc gggccgcgcc
     1321 gacatgttcc gcatggccga ggcgatgacg cggcacacct cggaggtcga cgtgctgcat
     1381 atcgggccgt acaagggctt cggcacccgc cacggcgtgc agcactgggc ggattcgtcc
     1441 aaacagccgc gcgtgtcgaa cgcggccttc aagcgcttct actactacct gaccgccgat
     1501 gagcgcgtcg gcgacctgat gcgcgatctc gtcacctccg accagacgct gaaggacgtc
     1561 tatatcgagc gcaaggtcgc ctcggcgggc ggctccggca tcggcatccg caagcacgtc
     1621 gaaggcacgg tcgattgcag cttcggcacc tcgtggggat cgttcatcgc ggcgtggctg
     1681 accgaatggg agcgcacgca ggacacgcgc tggcgcgacc ggatcctgac cggcatgaac
     1741 tcgatcgcga aactgaaata cggctggttc tgcggcggcg cgccctatga cctgaaaacc
     1801 ggcaaattcg tcgggccggg cgatcaggtt tcggtgtcgc acctgaacgc ggtgttcggc
     1861 gtggtcgaga tcaccgagga gttgctgacc ctgatcgacg ccccggccta caagaaggtc
     1921 tggctggatt attgtatata ttacaacagc tcgccggagg aaattaaagc gtttctgggc
     1981 gtcgttccca aggaccgcag ccttcgcgac gcccattcgc gcctgaccgc ctatgcggcg
     2041 cgcgagctga aagacccgaa gctggccgcg cgcgcgtgga gcgagttctt cgacggcgac
     2101 aaccgccgtt tccgcacccg cctgaatccg caggaggaca aggtgacggg gccggacgtt
     2161 ctgtacgcca tcgaggaaga cccggcgatc tcgaccaacg gcgcctcgca atgggggctg
     2221 gccgccatcg agaatctgca actgatcggc gacagcctcg aggccaacgc gccgaagggt
     2281 tgagcgcgga acggggaggc gtgatccgcg cctccccgcc ttatgaaaaa ttcatatatt
     2341 cggcggttat attatatcgg cgccaatata tccgacgctc tagcatgaac gccatagcga
     2401 ggagcgtggc gtatgactca tcaccggcgg aacgtcgcca ttaccggcgc ggattcaggc
     2461 ataggcgcgg cctgcgcgcg cgcgttcggc gcgctgggcg accatgtcac tgtcttttat
     2521 taccacgaca aggacggcgc cgaggccagc gtccgcgcgg tcgaggcggc gggcgggcag
     2581 gccgtcgcga tccagtgcgc ggtcaacgac cgggactccg tcgacagggc ctttgcggac
     2641 atggaggcgc gctatggccc ggcgcgcgtc ttcgtcaatt cggcgggcgt caacatgcgc
     2701 ggcgtgcggg tgcgcgacat ggcgccggag gtctggctgc gcacggtcgc gaccgatctg
     2761 accggcgcct acctgacctc gaagcggttt ctgcaaagct gcccgcgcgg cccggcgtcg
     2821 atcgtgcata tctcctcgat ccacgccgag gcggtgcgcg acggcggcgc cgactattgc
     2881 gccgccaagg ccgggctgaa caatctggtg cgcacgctgg cgatcgagga ggcggccaac
     2941 gccatccgcg tcaacgccat cgcgcccggc atgatcctga cgccgatgaa ccagcgcgcc
     3001 gtcgacgacc ccgattatcg ccgcaagctg gagcgccaca ttccgatggg ccgcgccggg
     3061 cgggccgagg aggtcgcgca tctggcgcgc tggctggcct ccgacgaggc cgcctacatc
     3121 accggcgcca gcctcgtgat cgacggcggg ctgtcgctgt tgcaggcggt cggcgcatga
     3181 cccggcaggc cgcccgctcg gcgccggacc cggtgaccgg cgaccgcaag gaacatgtcg
     3241 tcaccgggcg cggcggcgtc gatttcaacg tcgaggcgct tcaggacgtg cggctgaacg
     3301 ggccgcgcgc gctgtgcgaa cgcgacctgc tcagccccta tgtctggcgc gcgccggacg
     3361 ggcggctcgg cctgatggtc agggccgcgc cccccgccgg caagccgcgc acggacaccg
     3421 gccagatctg ggcgggctgg agccatgacg gggtgacctt cgacatgctg gacgcgcccg
     3481 tgctgaccgg cgggccggag gacaccgaca taggcggcgt cgaggatccg accgtgctgc
     3541 tgcgtgagga cggctcctat gtcgtctatt acaccggcgt cgggccggac atgacgcacg
     3601 gcgagatgtt ctacgccacc ggcccgtccg tcgaccggct gagcaagacc ggcgtggcgc
     3661 tggcctcgac caagtcgatg ggcaacacca aggaggccac ggtcgggcgc acggcggacg
     3721 gccggtggcg gctgttctac gaatacgcgg cgcagaacgc ctcgcgcgtc ggcctggccg
     3781 tcggccccga cgtcggcggg ccgtggcatg agcgcccgac gccgttcatg ccgcgcgagg
     3841 acggctggga caactggcac ctgtcgcccg gtccgatgct gatggaccgg cccggcgagc
     3901 cggtgatgtt ctacaacggc gccacgcgcg acgcccgctg gcgcatcggc tggatcgcct
     3961 tcgaccgcga ttgccgcacg gtcgtcgacc gctgcatcga accgctgctg acgccgccgc
     4021 cggtcgagga ccggaccgcc accgacatcg ccttcgccgc ctcggtcatc gtgcaggacg
     4081 gcgagatctg gctctattac tcgctcgagg accgccgtct ggcgcgcgcg cgcctccggc
     4141 aaagctgaaa aggaaggacg ccgacatgca cggacacaaa cgctggacca tccccgaagg
     4201 ttacctgccg cccgacgatc cggccaagcc gcgccctctg gtcagccacg aagccttctg
     4261 cgtgctgaat ccgggcgatg acgacgccga tatcgaggtg accatctatt acggcgaccg
     4321 cgagccggtg gggccgtacc ggatgcgggt cgcggcgcgg cgcaccgtgc atttccgctt
     4381 caacgacctg aacgaccccg agccgatccc ggccggcgtc gattacgcca gcgtcatcac
     4441 cgcctcggcg ccgattctgg tgcagcacac gcggctcgac agccgcagcg ggacgatcgc
     4501 gctgatgacc accatggcga tcgcgggata ggcggcgatg acggccttcc gcgagcgcgg
     4561 cgcggcggcg cagatggacg accggccgct acggtcgtac tacaccggcg cgaacccggc
     4621 tctggccgtg gatatcgacg acctgcgcgc gcgggcgcac cggctgacgc cgcgcttcgt
     4681 gctggaatat ctcgaaggcg gcgcggggca ggagacgacg ctggcgcgcg agcgcgcggt
     4741 ctatggcgag tggctgttca tgccgcgcac cctgatggac gaggcgcgcc gcgacctgac
     4801 gcggccgctg ctggggcggg acgcgaagat gccgctgctg gtcgggccga ccgggctgaa
     4861 cggcctgttc cggcgcgggg ccgacctcga actggcgcgc gcctgcgccc gcttcggcct
     4921 gccgttcgtg caaagcacca tgtccaacga acgcatggag acggtcgccg gggtcgaggg
     4981 gctgcgccac tggtggcagc tttacgtgtt cggccccgac gaaatctggc agtcgctggt
     5041 ggaccgcgcg gcggcgtgcg gctgcgaggc gctggtcgtg acgaccaatt cgcaggtgta
     5101 cggccagcgg caatggagca cgcgcacccg cagcgagcag gccctgccgc cgcccgccgt
     5161 cctgttcgac atgctgcgcc atcccggctg gctgctggcg acgatggcgc gggggatgcc
     5221 ggaattttcc aatgtcgtcg actatatccc gaaggcgcat cgcggctttt tcgacagcgc
     5281 ctactggatt cgcggccaga tgcccgccgc cctgtcgtgg cgcgacatcg cgcgcatccg
     5341 cgcgcgctgg aaaggcccga tgttcgtcaa gggcgtgctg aatctggagg atctcgatcc
     5401 ggcgctggac agcggcgtcg acggggtgat gatgggcagc cacggcggac ggcaggccga
     5461 cggcgcggta tcggcgctcg acctgctggg gcgcgcgaag gacagggtcg gcgaccggct
     5521 ggcgctttac gtctccggcg ggctgcggca cggcgcggac ctgctcaagg cgctggggct
     5581 gggcgccgac gcggtcatcg ccgggcgcgc gccgctttac ggcctgtgcg cctacgggga
     5641 ggcgggcgtt tatcgcgcgc tggagatcct gcgcgacgag gcgctcaacg agatggccca
     5701 gatgggcgcg ccgtcgcccg atcagcttgg ccgcctgctg atccgccgcg accgcctgcc
     5761 gttatagtca gtaaacgaac accttttgcg ggcgcagcct gagcgcggcg tcgcgaacat
     5821 aggcgaggtc ggcggcgcgc gattgttcgg tctcgaacag cgcgccgtcg tcgccgcgca
     5881 gctccagacg cagcgtgccg cccgccgcca gcacggcgct gaccgtgacg ggcaggccgg
     5941 ggccttcgcg gctgaggtcc gcctcgaacg ggcggaagcg cgcgacatag tcgccgtccg
     6001 cgaccggcgc ggccagcggc gcgtcgaagc cgtcgaacag cgcgcgcccg tccctgacgc
     6061 ggcacggcag ccggttggcc gcgccgagga agtcgaacac gaaggccgag gcgggcgcgt
     6121 cgaaaatgtc cagcggcgtt ccgatctgct cgatccggcc gtccttcagc accacgacgc
     6181 ggtcggcgag gtcgagcgcc tcctcctgat cgtgggtgac gaagatcgtg gtcacgccgg
     6241 tttcgttgtg gatgtggcgc agccagcggc gcaaatcctt gcgcaccagc gcgtcgagcg
     6301 cgccgaacgg ctcgtccagc aacagcatgc gcggttcgat ggccagcgcg cgcgccaaag
     6361 cgacgcgctg acgctggccg ccggacaact ggctgggata gcgcttgccg aggccgtcga
     6421 gctgcaccag cttcagcagg cgctcgaccg tcgcgcggat ctcggccttg ggcggtttcg
     6481 ccttgccgga acggacgtcg aggccgaagg cgatgttctg cgccacggtc atgtggcgga
     6541 acagggcgta ggactggaac accatgccga cgcggcgctg gcgcggatcg agcttcagat
     6601 agtcctcgcc ctgaaaggtt atggcgcccg aatccggcgc gtccagcccg gccagcaggc
     6661 gcaacagcgt cgtcttgccg gagccggacg ggccgagcag ggcgatgaat tcgcccggct
     6721 gcacgtcgaa gctgatgtcc ttgagcgcgg cgaagccgga aaaggtgcgg gtgatgttgt
     6781 ggatgctgag ggtcatcagt gcctccggtt ggcggccagt tcgccggcga aacgccattc
     6841 gagcagggtt ttgaggatca gggtgacgag cgccagtccg gccaggatcg aagccgccgc
     6901 aaaggcgccc actgtgtcgt actggttgta ggcgatctcg atttgcagcg gcagggtgtc
     6961 ggtcaggccg cggatatggc cggacacgac gctgaccgcg ccgaattcgc ccatcgcccg
     7021 cgccgtgcag agcaagacgc cgtaggtcag cgcccacttg atgttgggca gggtgacgcg
     7081 gaagaagatg tcccagccgc gcgcgcccag cgtgcgggcc gcgacctcct cgtccgtacc
     7141 ctgatcctcc atcagcggga tcagctcgcg cgccacgaac ggcagggtga ccagcaccgt
     7201 ggccagcacg aggccgggca gggcgaacac gatcttgacg tcgtggtcgc gcatcaggaa
     7261 gccgaaccag ccctgcgcgc cgaacagcag aatccagacg aggcccgcta tgaccggcga
     7321 cagcgaaaac ggcaggtcca gcacggccag cagaaggctc ttgcccggaa actcgaagcg
     7381 cgtgatcgcc caggcggcgc acaggccgaa gcccgcattg agcggcacgg agaggacggc
     7441 gaccagcagg gtcagtttga tcgcgtcgat cgtgtcttca ttgccgagcg cgctgagcca
     7501 cggcgccacg cccttggcga aggcggtcgc cagcacatag accagcggca gcacgatcac
     7561 gacgccgagc cagatcaggg tgaagccgag cagggcgatc gcgcccgcgg ttttcggcgg
     7621 tttggcggcg cgggcggtca tgcggcggct ccccagcggg cgaagcggcg ctggatcagg
     7681 ttcagcagca ccagaatgag cagggacagg gccagcatga ccacgccgac agcggcggcg
     7741 cccgcatagt cgaactcctg aagcttgatg acgatcaaaa gcggcgcgat ctccgagaca
     7801 tagggcatgt tgcccgcgat gaagatcacg ctgccgtatt cgccgacgcc acgcgccagc
     7861 gccagcgaga agccggtgac cagcgacggc atgagcgcgg gcagcacgat gcgcgtcagg
     7921 ctctgccagc cgttcgcgcc gagggtctgc gcggcctcct cgatctcggc ctcgaactcg
     7981 gccagcaccg gctggaccga gcgcacgatg aagggcaggc cgataaagac cagcgccacg
     8041 aagatgccga gcggcgtata ggcgaccttg aggccgaccg cgttcagggc ctgacccagc
     8101 cagccgttgc ccgcatagag gctggacagg gcgatgcccg ccacggcggt cggcagggcg
     8161 aagggcaggt cgataaaggc gtcgagcagg cggcgtccgg ggaaagaata gcgcgtcagg
     8221 ctccacgcga ccgccagccc ggcgaagacg ttgacgaagg cggcggccag cgcgatcgag
     8281 aagctgatcc aaagggcgtg cagcacgcgc ggatcggtca gcacgaacat cacccccgtg
     8341 aggccgtgct cccacgggcg cgcgatcagg gcgatcagcg gaatgaccac catgacgctg
     8401 aggtagaaga ccgtcaggcc gagcgtgatc ctgaaaccgg gcagcacgcg cgtgcgcaca
     8461 tgcaggccgg gggctttgaa gtcgtccggc agagccggag cggagtcggc cgaagcggag
     8521 tcggccagag cggagtcggc cagagcggag tcgggttgtc ccgtcatgag aattcctgtc
     8581 ggttgggtgt cttcatcctc ccccgttcac gggggaggtg gcgagcttgt ctcgccggag
     8641 gggggggggg gcgctcgcgg ctcccccttc cgtctctcct tcgctaacgc tccggagagc
     8701 caccttcccc gtaatcgggg aaggatgagt agtatcgaca ttattttcgg gtaatctgat
     8761 cgaacacccc gccgtaatcg aactgctcgg cctgcacctt cttccagccg ccgaagtcgc
     8821 ggatggtgac gaggttgagc gccgggaact ggctggcgta tttggcggcc accgtcttgt
     8881 cgatcgggcg gtaatagttc ttcgcgatga cctcctgcgc ctcggggctg tagagatagg
     8941 acaggtattc cctggcggcg gccagattgc cgttcttcag cgcgttcttg tccaccacgg
     9001 ccaccggcgg ctcggccagg atcgagatcg acgggaccac gatctcgaac ttgcccttgc
     9061 cgatgtcgtt gagcgcgagg aaggcctcgt tctcccacgc gatcagcacg tcgccgacgc
     9121 cgcgctcggc gaaggtcacg gtcgagccgc gcgcgccggt gtcgaggacc ggcacgcgat
     9181 tgaacaggtt tttcaggaat tcgcgcgccc tggcgtccgt gccccagcgc tttttcgcat
     9241 aggcataggc ggccagatag ttccagcgcg cgccgcccga cgttttcgga ttcggcgtga
     9301 ccaccgagac gtcgagccgg atcaggtcgt cccagtcctt gatctttttc ggattgccct
     9361 tgcgcaccag aaacacgatg gtcgaggtgt agggcgtcga atagttgggg aagcgcgact
     9421 gccagttggc gggcagcagc ccggccttct gcgagatcgc gtcgatgtcg gcggccaggg
     9481 ccagcgtcac cacgtcggcg ggcgcgccgt cgatgaccga gcgggcctgc ccgccggagc
     9541 cgccgtggct ctggcgcacg gtgatgtcct cgccggtctt ctgcttccag tgctggatga
     9601 acaggggatt atactgggcg taaagctcgc gcgtcgggtc gtaggagacg ttgagcagtt
     9661 cgcgggtctt gcccgccgcc agtgtgacgc cgggcagggc gcccgacgcg gcggccaggg
     9721 cggcgcctcc cgcgagggtc ttcaggaagg atctgcgttg catgagaggt atccttgtga
     9781 gggggtgtga aaacgcgatc agtaacggaa ctggacgcgg gcggcggtga agtcttcggt
     9841 cctgcggtcc gcgccgccgg ctgcgccgcc ctcgaacttg gtctggtcgt agtcgagcag
     9901 cagcttgagg ttctcgctcc agtaccagcc gaggccgagc cctgtgtcgg tcgccttgcg
     9961 cgccgaactg ccgatcgagg cgaagccgcc ggtgaaggcg tccttgtcga tgtcgttata
    10021 accgtagcgc gccgtcagct cccacgcgcc ccagccgccg ttcttcaggc tgaaatcatg
    10081 cgcgggcttg acgccgccct tatagcccac gctctcgccg gtcagcaccc acgagccgga
    10141 cacgtcgagc gcggtgtt
//
LOCUS       DASVLL010000439         4530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_459316,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000439 DASVLL010000000
VERSION     DASVLL010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459316"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF402_07300"
     CDS             <1..411
                     /locus_tag="VF402_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800139.1"
                     /translation="AYMRLLCVEDNSTLRATVDLMLAPAGIDVDFATDGAEAVEAWQF
                     NEYDAVLMDIEMPGMNGLAAVRAIRRMEDGFHLGYTPVLFLSSYDDEAHVERADEAGG
                     DGFLAKPFTTETLLGALDGLMRQRRGGDLSLAAQ"
     gene            552..1583
                     /locus_tag="VF402_07305"
     CDS             552..1583
                     /locus_tag="VF402_07305"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7800140.1"
                     /translation="MGYRVAVVGATGAVGREMLAILEEVNFPVSEIHAVASRKSMGIE
                     IGFGNKSLICQNLETFDFSKVDIVLMSAGGDVSKAWSPKIGAQGPIVIDNSSAWRMDP
                     DVPLVVPEVNPDAVWDCKKKHIIANPNCSTIQMLVVLKPLHDAATIKRVVVSTYQSVA
                     GAGKAGMDELWDQTKAIYGLGDKEPKKFTKQIAFNVIPHIDVFMEDGYTKEEWKMIVE
                     THKILDPTIDVVPTAVRVPVFVGHSESLNIEFENPMDEQLAREILREAPGVAVVDKRE
                     NGGYVTPIEVVGEFDTYVSRIRNDPTVPHGISLWCASDNLRKGAALNAVQIAQLLDER
                     GVIEARADA"
     gene            complement(1773..3389)
                     /locus_tag="VF402_07310"
     CDS             complement(1773..3389)
                     /locus_tag="VF402_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7800141.1"
                     /translation="MPALLSLLRRFCRQRRGNVTLIFGLSALTLIAVTGGALDVSGMS
                     DTRAALQDAADAAALRGALTYNQGLSASQTAANTAFLQNFQSDKVEGLQYNLSANVSG
                     NITQLTYTASAQYKPLFLGLLGVGAQTLSAESKAQSQVNPFELVFVLDTTGSMAGTRI
                     SNLKSAVGSVLDTLYDMEGDNSSDVKIGVAPFDTQVRVASGSSYAYIDYSNCASHEDG
                     YYTGGGWYGQTYVPGIDYHACLAVWDVYNSICATAKSPSACQSTAASALYYKTSTSDG
                     VTYYTALGYANEKISKNNYAIYSYTETYYIDPATFKKVVTQSGLTTTSAKNSKSYVGP
                     TSGYSNFPSSDLDNVAASWSGCLSDRSQNFDVSAQSADAVMPDTLYVAAGCTQSNLTT
                     VKDMTNNITSVKSYVNGLTTNGNTNITVGLQFGMEMLSPDAPYTNGVAWTDDDVDKYM
                     ILVTDGANTQNRWSSTTGDIDARTKLACQAVEDRGVTLFVVNIIEGDSTLLENCASQT
                     SYYYNLTSASQLGDAMQDILKSIGKLRLVH"
     gene            complement(3483..>4530)
                     /locus_tag="VF402_07315"
     CDS             complement(3483..>4530)
                     /locus_tag="VF402_07315"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7800142.1"
                     /translation="RELGARFTAQIAGETVVDLWGGQADREGTRAFDAATLTPVFSTG
                     KAVMALLIARAVDQGKLDYEAPVASYWPDYGQNGKDKVTLGQLISHQDGLPGFYPPQD
                     PSIWFDASATLKALCEQAPLWTPGDGSGYHPITGGYLLGEVFRRADGRSLGTALREDF
                     AEPFGVEVMIGTPDVYAPRIAHLQKPSAPPDLGPIDRIKSSAFLDKGSAPAGRGSADW
                     RRMEIPSANAHATAPGLARLMSVVANGGTLDGQALISPQTLTQATRERCYGQDRVLPY
                     RLSWGAGFLRNKGLNIYGPNARAVGHSGWGGSCALADPERKVSAAYVMNKQSPYLLGD
                     PRPVALIDAFYGCL"
BASE COUNT          803 a         1390 c         1503 g          834 t
ORIGIN      
        1 gcgtatatga ggctattatg cgttgaagac aattccaccc tgcgcgccac ggtcgatctg
       61 atgctggcgc cggccgggat cgacgtcgat ttcgccacgg acggcgccga ggcggtcgag
      121 gcgtggcagt tcaatgagta tgacgcggtg ctgatggaca tcgaaatgcc cggcatgaac
      181 ggactggcgg cggtgcgcgc catccgccgc atggaggacg gcttccacct cggctatacg
      241 ccggtgctgt tcctgtccag ctatgacgac gaggcgcatg tcgagcgcgc cgacgaagcg
      301 ggcggcgacg gctttctggc caaacccttc accaccgaga ccctgctggg cgcgctggat
      361 ggcctgatgc gtcagcggcg cggcggcgac ctgtctttgg cggcccaata aggcgaaaac
      421 gccgcccgac acggccaaga cggcgccaag acggtgattg tggacagttt ggacttgtca
      481 tcccggcgaa aaaggcccat gtgccgccca ttcgggccta tgcgcctttt ttgctcaggg
      541 agttcagcat catgggttat cgggtcgctg tcgtcggcgc caccggcgcc gtgggtcgcg
      601 agatgctcgc catcctcgaa gaggtcaatt ttcccgtttc ggagatccat gcggtcgcct
      661 cgcgcaagtc gatggggatc gagatcggct tcggcaacaa gagcctgatc tgccagaatc
      721 tcgaaacctt cgatttttcc aaggtcgaca tcgtgctgat gagcgcgggc ggcgacgtgt
      781 cgaaggcatg gtcgcccaag atcggcgcgc agggtccgat cgtcatcgac aactcgtcgg
      841 cctggcgcat ggacccggac gtgccgctgg tcgtgccgga ggtgaacccg gacgcggtct
      901 gggactgcaa gaagaagcac atcatcgcca acccgaactg ctcgaccatc cagatgctgg
      961 tcgtgctgaa gccgctgcat gacgccgcca cgatcaagcg cgtcgtcgtc tcgacctatc
     1021 agtcggtcgc gggcgcgggc aaggcgggca tggacgagtt gtgggaccag accaaggcca
     1081 tctacggcct cggcgacaag gagcccaaga agttcaccaa gcagatcgcc ttcaacgtca
     1141 ttccgcacat cgacgtcttc atggaggacg gctacaccaa ggaagaatgg aagatgatcg
     1201 tcgagacgca caagattctc gacccgacca tcgacgtcgt gccgaccgcc gtgcgcgtgc
     1261 cggtgttcgt cggccattcc gaatcgctca atatcgagtt cgagaacccg atggacgagc
     1321 agttggcgcg cgaaatcctg cgcgaggcgc ccggcgtcgc cgtggtcgac aagcgcgaga
     1381 acggcggcta tgtcacgccc atcgaggtgg tcggcgaatt cgacacctat gtctcgcgca
     1441 tccgcaacga tccgactgtg ccgcacggca tttcgctgtg gtgcgcctcg gacaatctgc
     1501 gcaagggcgc ggcgctcaac gcggtgcaga tcgcgcaatt gctggacgaa cgcggcgtga
     1561 tcgaggcgcg ggcggacgct taaagcgttt ccccaaaaag tggttccact ttttggataa
     1621 ggaagagcga caaaacaaag acttagagcg ccgatctgat gcaatcagat cgaaaagcgc
     1681 tctaaagccg gttacagggt gacagaaaac cggcgggcga tgcgcgccgg tttttttgcg
     1741 tcgccggttt tttgcgtcgc cgtttttttg cgtcaatgca ccagccgcag cttgccgatg
     1801 ctcttgagga tgtcctgcat ggcgtcgccg agctgcgagg ccgaggtcag gttgtagtaa
     1861 taggatgtct ggctggcgca gttttccagc agggtcgaat cgccttcgat gatgttgacc
     1921 acgaacaggg tgacgccccg atcctcgacc gcctgacagg ccagtttcgt gcgcgcgtcg
     1981 atgtcgccgg tggtcgaact ccagcggttc tgcgtattgg ccccgtcggt gaccaggatc
     2041 atgtacttgt cgacatcgtc gtccgtccag gccacgccgt tcgtataggg cgcgtccggc
     2101 gacagcatct ccatgccgaa ttgcaggccg accgtgatgt tggtgttgcc gttggtggtc
     2161 aggccgttga cgtaggattt gaccgaggtg atgttgttgg tcatgtcctt gaccgtggtc
     2221 aggttgcttt gcgtgcaacc cgccgcgaca tagagggtgt cgggcatgac cgcgtcggcg
     2281 ctttgcgcgg agacgtcgaa attctggctg cggtcgctca ggcagcccga ccacgaggcc
     2341 gcgacattgt cgaggtcgct ggacggaaaa ttgctgtagc cgctggtcgg gccgacatag
     2401 gacttgctgt tcttggcgct ggtcgtggtc aggccggact gcgtcaccac cttcttgaag
     2461 gtcgccggat cgatatagta ggtctcggta tagctataga tggcgtagtt gttcttgctg
     2521 atcttttcgt tagcgtagcc gagggcggtg taataggtca cgccgtcgct ggtcgaggtc
     2581 ttgtaataaa gcgcgctggc ggcggtggac tgacacgccg acgggctttt ggccgtggcg
     2641 cagatgctgt tgtaaacgtc ccagaccgcc agacaggcgt gatagtcgat gccgggcaca
     2701 taggtctgac cataccagcc gccgcccgta taatagccgt cctcatggct ggcgcagttg
     2761 gaatagtcga tataggcata gctcgaaccg gaggcgacgc gcacctgggt gtcgaagggc
     2821 gcgacgccga tcttcacgtc gctgctgttg tcgccttcca tgtcgtaaag cgtgtccagc
     2881 accgagccga ccgccgattt caggttgctg atgcgcgtcc ccgccatcga gccggtggtg
     2941 tcgagcacga agaccagctc gaacgggttg acctgcgact gcgccttcga ctcggcgctc
     3001 agggtctggg cgccgacgcc cagcaggccc agaaacagcg gcttgtactg cgccgacgcc
     3061 gtataggtca gttgcgtgat gttgccgctg acattggccg acaggttgta ttgcaggccc
     3121 tcgaccttat ccgactggaa attttgcaga aacgccgtgt tggcggcggt ctggctggcg
     3181 ctcaggccct gattgtaggt cagggcgccg cgcagggccg ccgcgtcggc ggcgtcctga
     3241 agcgcggcgc gcgtgtcgct catgcccgat acgtcgagcg cgccgccggt gacggcgatc
     3301 agcgtcaggg cgctcagccc gaagatcagg gtgacattgc cgcgtctttg ccggcaaaat
     3361 cggcgcagca gggaaaggag cgcgggcatg gcgtcgacca tacgagggat agggcccgcg
     3421 actatgcccg cacaacgtta atttagcatg acgcgacagg gtaaacggcg cggcggccgc
     3481 ggttaaaggc agccgtaaaa cgcgtcgatc agcgcgaccg ggcgcggatc gcccagcaga
     3541 tagggcgact gcttgttcat cacataggcc gccgacacct tgcgctccgg gtcggccagc
     3601 gcgcacgagc cgccccagcc gctgtggccg acggcgcgcg cattggggcc gtagatgttc
     3661 agccccttgt tgcgcaaaaa gcccgcgccc cacgacaacc ggtacggcag cacgcggtcc
     3721 tgaccgtagc agcgctcgcg cgtcgcctgc gtcagggtct gcggcgagat cagcgcctgc
     3781 ccgtccagcg tcccgccgtt ggcgaccacg ctcatcagcc gcgccaggcc cggcgccgtg
     3841 gcgtgggcgt tggcggacgg aatctccatg cgccgccagt ccgccgaacc gcgccccgcc
     3901 ggggccgaac ccttgtcgag gaaggccgat gacttgatgc ggtcgatggg gccgagatcg
     3961 ggcggcgcgg acggcttttg cagatgcgcg atgcgcggcg cgtaaacgtc cggcgtgccg
     4021 atcatgacct cgacgccgaa cggttcggcg aaatcctcgc gcagcgcggt ccccagcgaa
     4081 cggccgtcgg cgcggcggaa cacctcgccc agcagatagc cgccggtgat cgggtgatag
     4141 ccggagccgt cgccgggcgt ccacaacggc gcctgttcgc acagcgcctt cagcgtcgcg
     4201 gatgcgtcga accagatcga cggatcctgc ggcggataaa agccgggcag gccgtcctga
     4261 tgcgagatca gttgccccag cgtgaccttg tccttgccgt tttggccata gtcgggccag
     4321 taggacgcca ccggcgcttc gtaatcgagt ttgccctgat cgacggcgcg cgcgatcagc
     4381 agcgccatca ccgccttgcc ggtcgaaaac accggcgtca gggtcgccgc gtcgaaggcg
     4441 cgcgtccctt cgcggtcggc ctgcccgccc cagagatcga ccacggtttc gcccgcgatc
     4501 tgcgccgtga agcgcgcgcc cagctccctg
//
LOCUS       DASVLL010000440         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_82995, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000440 DASVLL010000000
VERSION     DASVLL010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82995"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..662)
                     /locus_tag="VF402_07320"
     CDS             complement(138..662)
                     /locus_tag="VF402_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEX7800143.1"
                     /translation="MTFKTVFVGGGAVLAAGFAVSASAGQVQIGVEIPQTSVAEFHKP
                     YVAGWIETAAGQPAGDVFVWYDVRKPNNAGAKWLSDLRTWWRKSGRSLNLPVDGLSGA
                     THAPGRQTLTLDSGASAFKGLQPGAYQLVVEAARENGGHSLVKVPFTWDGKAFSAAGR
                     TTGELGAVTVSYKP"
     gene            complement(672..1289)
                     /locus_tag="VF402_07325"
     CDS             complement(672..1289)
                     /locus_tag="VF402_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX7800144.1"
                     /translation="MSERSPVLSPFWRNQLRQWHWISSALCLIALLLFSVTGFTLNHA
                     AQISARPKTTHVEKDLPATDAALIAQAKDGQPLPAGLRAAVKALTGVAAGKAKAQVSD
                     GEIYVDLTTPGVDNALTIEDDGHVTWERTDRGLIAVLNDLHKGRHAGPVWSLFIDLIA
                     IACVVFAVTGLGLLWLYEKHRRVTWPLVAFGALLPFIIFMLFVHS"
     gene            1483..>1918
                     /locus_tag="VF402_07330"
     CDS             1483..>1918
                     /locus_tag="VF402_07330"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7800145.1"
                     /translation="MDRRAVLKTLGAMAAAAATPFAANAQNAARPGRFTAADDGFLLD
                     GQPFQVLAGEMHYPRIPRDSWRDRLRKLKALGLNTLTTYVFWNAHEPKPGVYDFSGNL
                     DVAAYVKLAQEEGLYVNLRPGPFVCAEWDGGGLPAWLFPTDDI"
BASE COUNT          331 a          630 c          621 g          336 t
ORIGIN      
        1 ctttcgccag gttccccctt ccgtcgtttg ctccgcaaac ggcgccttcc ccgtttacgg
       61 ggaaggcgag agtctttatt cgccctttct gcgaagcggt gggaggggcg tccttcatat
      121 tcggaagcgt catcggttta cggcttgtaa ctcaccgtca cggctccgag ttcgcccgtg
      181 gtccggcctg cggcgctgaa cgccttgccg tcccaggtga agggcacctt gacgaggctg
      241 tggccgccgt tttcgcgcgc cgcctcgacc accagttgat acgcgcccgg ttgcaggccc
      301 ttgaaggccg acgcgccgga atccagcgtc agggtctggc ggcccggcgc gtgggtcgcg
      361 cccgacaggc cgtcgaccgg caggttcagg ctgcgcccgc tcttgcgcca ccaggtgcgc
      421 aggtcgctca gccacttggc gcccgcattg ttgggcttcc tgacgtcgta ccagacgaat
      481 acgtcgccgg cgggctggcc cgccgccgtt tcgatccagc ccgcgacata gggcttgtga
      541 aattcggcga ccgaggtttg cggaatctcg acgccgatct gcacctgacc ggcgctggcc
      601 gacacggcga atccggcggc cagaaccgcg cctccgccga cgaaaactgt tttaaacgtc
      661 ataagttacc tctaactatg aacaaacaac atgaagatga tgaagggcaa aagcgcgcca
      721 aacgcgacca gcggccaggt gacgcggcga tgcttttcgt acagccacaa cagccccagc
      781 cccgtcaccg cgaacaccac gcaggcgatc gcgatcaggt cgatgaacag gctccagacc
      841 ggcccggcgt ggcgcccctt atgcaggtcg ttgagcacgg cgatcaggcc gcgatccgtg
      901 cgctcccacg tgacatggcc gtcgtcctcg atggtgagcg cattatcgac gccgggcgtg
      961 gtcaggtcga catagatctc gccgtccgac acctgcgcct tcgccttgcc cgccgcgacg
     1021 ccggtcaggg ccttgacggc ggcgcgcaaa ccggcgggca ggggctggcc gtccttcgcc
     1081 tgcgcgatca gggcggcgtc ggtcgcgggc aggtcttttt cgacgtgcgt ggtcttcggt
     1141 ctggccgaaa tctgcgcggc gtggttcagc gtgaagccgg tgaccgagaa cagcagcagc
     1201 gcgatcaggc acagggccga actgatccag tgccactgac gcaactgatt gcgccagaac
     1261 ggggacagca cgggggaacg ctcggacatg ggacggcggg tccataattg ataatcattc
     1321 tcattatcaa ccgcatccgc gcggcgcaag cccgttttcg gcccgcgcgc gattgatttt
     1381 gcggcaggtc cgaaaagcgg gccgcgaaaa ttcgggcttg aggcgcgacg cgattgtgct
     1441 aattgtcata caataaataa tcccgccaca aaggaaacgc cgatggaccg ccgcgccgta
     1501 ctgaaaaccc tgggggcgat ggccgccgcc gccgcgacgc ccttcgccgc caatgcgcaa
     1561 aacgcggcgc ggccggggcg cttcacggcg gcggacgacg gtttcttact ggacggccag
     1621 ccgtttcagg ttctggccgg tgagatgcac tatccgcgca tcccgcgcga ttcgtggcgc
     1681 gaccggctgc gcaagctgaa ggcgctgggg ctgaacaccc tgaccaccta tgtgttctgg
     1741 aacgcgcacg agccgaagcc gggcgtgtac gacttttccg gcaatctcga cgtcgccgcc
     1801 tatgtgaagc tggcgcagga ggaggggctt tacgtcaatc tgcgccccgg tccgttcgtg
     1861 tgcgccgaat gggacggcgg cggcctgccc gcgtggctgt tcccgaccga cgacatcg
//
LOCUS       DASVLL010000441         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_404029,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000441 DASVLL010000000
VERSION     DASVLL010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404029"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..414)
                     /locus_tag="VF402_07335"
     CDS             complement(145..414)
                     /locus_tag="VF402_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800146.1"
                     /translation="MTTPITPSLSHLLNPQAAPPRPAAQGGGFASALAAQKAFFQQVT
                     APKMTPATYTAADIQKAAPQPSAPLPATGSDQPVRRPGSFLNIVV"
     gene            510..2045
                     /locus_tag="VF402_07340"
     CDS             510..2045
                     /locus_tag="VF402_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800147.1"
                     /translation="MPTLRAAALPSRNPAFGAFRGLAARRPLLVAAAALLLWTLLIRL
                     PFLHVIHDDEAFYAVVAQRWLRGEWPYAASYDVKAPGVFAVLAAVQAVFGAHLAVIKG
                     VEIAFTAWGALALYRLLRPVAGPVCAGIAAGAYPLYSLFMLGVAAPCQLIEAAFTIEA
                     FALALAADRAGRRRLVLMALSGLMMGCAVMTKQTAAFTALGLFGWAAWRSRARRDLLP
                     LALFCAAGLLPALGFAVCFLACGHVRDAFDAVVVNALSRSQTNMSRPSPLGVLQRVAQ
                     FPAFLKPILVISVGTLLALLRLPRLRAAVGGAPLSLLLVWSLSEALGILACRSPDIWY
                     GFVLIPPFLALAAIVLRHGVAFRPDQRAAWIGAYLLAAAAQPFLVPDAIAPSSLADYT
                     GSVRAAGALRMAGLKPGDNLLVTGRGHYAYLMTGALPKAKYFNAMHLMCAFPTPDADP
                     LAAAFATRPDFVLVSDLEFYISCQRPDRAAALNAELAAHYQAVSHVSGRWDSFTVYRR
                     RDP"
     gene            2089..>3424
                     /locus_tag="VF402_07345"
     CDS             2089..>3424
                     /locus_tag="VF402_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800148.1"
                     /translation="MLSASTPALSFAPVVTGWRRLRRARPNLILLAAILVWTCVTRLP
                     LLHVVQDDEAFYSVVASRWLRGELPYVASFDVKAPGVFALFAIVQAVFGASLYVVKGV
                     EIVFVALGALGLYRLLARHATARSAWWAAFLYPLYCLTLNGVGSPCEIVQAALKIWAF
                     AWLADAMLAQGEANRIRFAVLSGLMIGLAVTLKQTAVFEGAALFGGLAWQAWKRREAW
                     PLALFAVAGAAPTLVFAAYFFVHGHLADLYRDVVTLAYRRSQMGPLLPPAPWYVELTQ
                     RCELLIYVMTPLAVVTLGVLLAALRRQRLARVGDGCVIGLGLIWYVAAMASIMINRAP
                     DPRYVTALIAPSLLLFSLVVCEGTDFSAPRRAAWLLALGLLAAGAPLVALRVSLQAAH
                     HSNDPDYTGMALAAKTLRTDGARPGDNLLVVSRGHYIYVLSGLLPRAKYFNAM"
BASE COUNT          447 a         1117 c         1155 g          705 t
ORIGIN      
        1 ggataagccg cgcaaaaagg acaagctggc caaaaagctg aaaaaactgc ccatgccgga
       61 actgtcgccg ggcgtgtcgg gcgaagacga cgaccgggtg catgcgccgg aagaaaaaac
      121 ggcttaaaat gccctgaaac ccgcttaaac cacgatattc aggaaagatc cgggacgccg
      181 gaccggctgg tccgaacccg ttgccggcaa aggggcggac ggctgcggcg ccgccttttg
      241 gatatccgcc gccgtatagg tcgcgggcgt catcttcggc gccgtcacct gctgaaaaaa
      301 cgccttctgc gccgccaggg cgctggcgaa tccgccgcct tgcgccgccg ggcgcggcgg
      361 cgcggcctgc gggttcagaa ggtgcgacag ggaaggcgtg atcggcgtgg tcataggcaa
      421 attatgccgt ggatcaggta tttaaatggt taacggccat aaaggtttgc gcaaacggat
      481 atttacgttt cgccgctagg atgcgcgcca tgccgaccct gcgcgccgcc gccttgccct
      541 ctcgaaatcc tgccttcggc gcctttcgcg gcctcgcggc gcggcgtccc ctgctggtcg
      601 cggcggcggc gctgctgctg tggaccctgt tgatccggct gcctttcttg catgtgatcc
      661 acgacgacga agccttctac gccgtggtgg cgcagcgctg gctgcgcggc gaatggccct
      721 atgccgccag ctatgacgtc aaggcgccgg gcgtcttcgc cgttctcgcc gccgttcagg
      781 cggtattcgg cgcccatctg gccgtgatca agggcgtcga gatcgccttc accgcctggg
      841 gcgcgctggc gctttaccgc ctgttgcggc ctgtcgcggg tccggtctgc gcgggcatcg
      901 ccgccggcgc ctatccgctc tattcgctgt tcatgctggg cgtcgccgcg ccgtgccagt
      961 tgatcgaggc cgccttcacc atcgaagcct tcgcgctggc gcttgccgcc gaccgcgccg
     1021 gacgccgccg ccttgtcctc atggccttga gcgggctgat gatgggatgc gccgtcatga
     1081 ccaagcagac ggcggcgttt acggcgctgg gcctgttcgg ctgggcggcg tggcgcagcc
     1141 gggcgcggcg cgacctcctg ccgctggcgc tgttctgcgc ggcgggcctt ttgcccgcgc
     1201 tggggttcgc ggtctgtttt ttggcctgcg ggcatgtccg cgacgccttc gacgccgtgg
     1261 tcgtgaacgc gctcagccgt tcgcaaacca acatgtccag gcccagcccg ctgggcgtgt
     1321 tgcagcgcgt ggcgcaattt cccgccttcc tcaaaccgat tctggtcatc agcgtcggaa
     1381 ccctgctggc gctgctgcgc ttgccgcgcc tcagggcggc ggtcggcggc gcgcctttgt
     1441 ccctgctgct ggtctggagc ctgagcgaag cgctcggcat cctggcctgc cgttcgccgg
     1501 acatctggta cggcttcgtg ctgattccgc cgtttctggc gctggcggcg atcgtgctgc
     1561 gccacggcgt cgcctttcgc ccggaccagc gcgccgcatg gatcggggcc tatctgctgg
     1621 ccgcggcggc gcagcctttt ctcgtgccgg atgcgatcgc gccgtccagc ctcgcggact
     1681 atacgggcag cgtccgcgcc gccggagcgt tgcgcatggc ggggctgaag cccggcgaca
     1741 atctgctggt gacggggcgc ggccattacg cctacctgat gaccggcgcc ctgcccaaag
     1801 ctaaatattt caatgccatg catctgatgt gcgccttccc gacgcccgac gcggacccgc
     1861 tggcggcggc cttcgccacg cgccccgatt tcgtgctggt ctccgatctt gaattttaca
     1921 ttagttgcca aagacctgac cgcgccgccg cgctcaacgc ggagttggcg gcgcattatc
     1981 aggccgtgtc gcatgtcagc ggccgatggg acagtttcac cgtctatcgc cgccgcgacc
     2041 cgtgagtgaa aaaggcattc tttaccgtta cagtctagga cggagcctat gctttccgct
     2101 tcgacgcccg ccttgtcttt tgcgccggtt gtgaccggat ggcgccgcct gcgccgcgcc
     2161 cgtccgaacc tcattctgct tgcggcgatc ctggtctgga cctgtgtgac gcgcttgcct
     2221 ttgctgcatg tcgtgcagga tgacgaggct ttttacagcg tcgtggcgtc gcgctggctg
     2281 cgcggcgaac tgccctatgt cgcgagtttc gacgtcaagg cgccgggtgt gttcgcgctg
     2341 tttgcgatcg tgcaggccgt gttcggcgcc agcctctatg tcgtcaaagg cgtggagatt
     2401 gtctttgtcg cgctgggggc gctggggctt taccggctgc tggcgcgaca tgcgaccgcg
     2461 cgttcggcgt ggtgggcggc gtttctgtat ccgttgtact gcctgacttt gaacggcgtc
     2521 ggctcgccgt gcgagatcgt tcaggccgct ttgaaaatct gggccttcgc ctggctggcg
     2581 gacgccatgt tggcgcaggg tgaggcaaac cggatccggt tcgccgtgct gagcggattg
     2641 atgatcggtc tggccgtcac cctgaaacag acggcggtgt ttgagggcgc ggccttgttc
     2701 ggcgggttgg cctggcaggc atggaagcgg cgcgaagcct ggcctttggc gctgttcgcc
     2761 gtggcgggcg cggcgccgac cctggtcttc gccgcctatt tcttcgtcca cggccatctg
     2821 gccgacctgt atcgggacgt cgtgaccttg gcgtatcggc gttcgcagat ggggccgctg
     2881 ttgccgcctg cgccgtggta tgtcgagctc acgcaacggt gcgagctgct gatctatgtc
     2941 atgacgccgt tggcggtcgt gacgcttggc gtgctgctgg ccgccttgcg ccgccaaagg
     3001 ttggcgcgcg tcggcgatgg ctgcgtcatc ggtctgggat tgatctggta cgtcgcggcg
     3061 atggccagca tcatgatcaa tcgcgcgccg gacccgcgat acgtcacggc cctgatcgcg
     3121 cccagcctgc tgctgttcag ccttgtcgtc tgcgaaggga ccgacttttc ggcgccgcgc
     3181 cgcgcggcct ggttgctcgc tctgggcctg ctggccgccg gcgcgccctt ggtcgccttg
     3241 cgggtctcgc tgcaagccgc gcatcacagc aacgatccgg actataccgg catggccttg
     3301 gccgccaaga cgctgcgcac ggacggcgcg cgtccgggcg acaatctgct ggtcgtcagt
     3361 cgcggccact atatctatgt gctgagcggc ctcttgccgc gcgcgaagta tttcaacgcc
     3421 atgc
//
LOCUS       DASVLL010000442         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_28569, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000442 DASVLL010000000
VERSION     DASVLL010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28569"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VF402_07350"
     CDS             <1..312
                     /locus_tag="VF402_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800149.1"
                     /translation="QTRDRKPHSDKNRPDKNRADRARLVEKVRDMDPAGVFEPRAEGP
                     RKGRGGKPFNQKGGQKGGQQAQKFGERKSHKPNLVKGNAAKSGFVKSGEGAQFKRRAK
                     A"
     gene            465..>1714
                     /locus_tag="VF402_07355"
     CDS             465..>1714
                     /locus_tag="VF402_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase/hydrolase"
                     /protein_id="HEX7800150.1"
                     /translation="MTAAPQISSAGTRLGAGVFARLLKLAAPLLALGMLMTANALTGC
                     TAREPQNNDPQAIVARTLDDHSWARPQEARVSHVDLDLNADFNKKILSGTATLTLVTE
                     ANAKQVILDTQALDIQGVTDAAGAPLKYTLGKGNEMMGKPLTIELPQGAQTVVVHYAT
                     QEGGKALQWLEPSQTAGKKLPFLFSQGEEINTRSWIPTQDSPAIRQTYAARVTVPSNL
                     VAVMSADRLTPKGEPVPGHPSLRAFRFDMKQPIPPYLIAIAIGDIGFRAEGPRTGVYA
                     ERAVLDKAAYEFADMGKMVDAAETLYGPYPWGRYDVLVLPPSFPFGGMENPTLTFVTP
                     TVLAGDRSLVSLVAHELAHSWSGNLVTNATWEDFWLNEGFTTYCENRIMEQVYGKDRA
                     DMLKVLGWQDLQGALKDLDEAGQA"
BASE COUNT          327 a          569 c          571 g          247 t
ORIGIN      
        1 cagacgcggg accgcaagcc ccattccgac aagaaccgtc ccgacaagaa tcgcgccgac
       61 agggcgcgcc ttgtcgaaaa ggtgcgcgac atggacccgg ccggcgtgtt cgaaccgcgc
      121 gccgaaggtc cgcgcaaggg ccgcggcggc aagccgttta atcaaaaggg cggccaaaag
      181 ggcggccagc aggcgcagaa gttcggcgag cgcaagagcc ataagccgaa cctcgtcaag
      241 ggcaacgccg ccaaatcggg cttcgtcaag tccggcgaag gcgcgcaatt caagcgccgg
      301 gccaaggcct gatccaggac gatacgccgc atcgctcccg ctccgcgtgc aaacggggag
      361 ccggggcgat gcggcatttt cacagttgtg tgatgaggcg gtttgccctt aaatagccgc
      421 gcacaaactg cgtgtgcgct cgccagtcag gattacgccc gaatatgacc gccgcccccc
      481 agatttcatc cgccgggacg cgcctgggcg cgggcgtatt cgcccggttg ctgaagctgg
      541 cggcgccgct tctggcgctg gggatgctga tgaccgccaa cgccctgacc ggctgcacgg
      601 cgcgcgagcc gcagaacaac gatccgcagg ccatcgtcgc gcgcaccctc gacgaccatt
      661 cctgggcgcg tccgcaggag gcgcgcgtca gccatgtcga tctcgacctg aacgccgatt
      721 tcaacaagaa gattttatcc gggaccgcga ccctgaccct ggtgaccgag gccaacgcca
      781 agcaggtgat cctcgacacg caggcgctgg acatccaggg cgtgaccgac gcggcgggcg
      841 cgccgctgaa atacacgctg ggcaagggca acgagatgat gggcaagccg ctcaccatcg
      901 aattgccgca gggcgcgcaa accgtggtcg tgcattacgc cacgcaggaa ggcggcaagg
      961 cgctgcaatg gctggagccg tcgcagacgg cgggcaagaa gctgcccttc ctgttcagtc
     1021 agggcgagga gatcaacacc cgaagctgga ttccgacgca ggattcgccc gccatccgcc
     1081 agacctacgc cgcgcgcgtc accgtgccgt cgaacctggt ggcggtgatg agcgccgacc
     1141 gcctgacgcc gaagggcgag ccggtgccgg gccatccgtc cttgcgcgcc ttccgcttcg
     1201 acatgaagca gccgatcccc ccctacctga tcgccatcgc catcggcgac atcggcttca
     1261 gggccgaggg gccgcgcacc ggcgtctatg ccgagcgcgc cgtgctggac aaggccgcct
     1321 acgaattcgc cgacatgggc aagatggtcg acgcggcgga gacgctgtac gggccgtatc
     1381 cgtggggccg ctacgacgtg ctggtgctgc cgccgtcctt cccgttcggc ggcatggaga
     1441 atccgaccct gaccttcgtc acgccgaccg tgctcgcggg tgatcgctcg ctggtgtcgc
     1501 tggtcgcgca cgaactggcg cattcgtggt cgggcaatct ggtcaccaac gcgacctggg
     1561 aggacttctg gctcaacgaa ggcttcacca cctattgcga gaaccgcatc atggagcagg
     1621 tgtacggcaa ggaccgcgcc gacatgctca aggtgctggg ctggcaggat ttgcaaggcg
     1681 cgctgaaaga cctcgacgag gcggggcagg cgga
//
LOCUS       DASVLL010000443         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_459875,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000443 DASVLL010000000
VERSION     DASVLL010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459875"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..>2779
                     /locus_tag="VF402_07360"
     CDS             55..>2779
                     /locus_tag="VF402_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7800151.1"
                     /translation="MFRVLYCLRDHHDWKLVALAAIVCLISSAVTVMMIARAGLTREA
                     ARKEVRRSYAGWVAASGAAAGFAIWATHFIAMLAYNPGVPMTFSPGLTLLSLLVAVAL
                     TAGGIEIGVSRAFRGSGLTGGAVIGAGIASMHFIGMAALDVPGRLVWAPDLVFVSIAA
                     GMALSAAAMWSVNRFRRHPVWRAAPLLTLAIICHHFVAMGAVTVLPDPARGVAGLSLS
                     PTAMAAALFCAAAAVMAVCMFAVIWGQRFDAMRADTDRRFRVLLEGLEDTLLFLLDRE
                     GRVLDWNNGSERNNRYPVGEIVGKIYGDVCALDAQFPLRYRAALAAAAAEGRAELEAL
                     IQDADGHRFWGQTTLRALRGDDGELLGFASITQDVTARKADQDRSFEATRNLDAALSH
                     MSQGLLLFDRNEKLVLANKRFLDIYGLDPARVTPGVSFRDLLVMLMATRDGAAPGPDE
                     IEAWHARNRALIAAPGGGDMVSDFFAHRTMSVSHRPMPDGGWVTTFEDITERRKAEAR
                     IAHMARHDGLTGLPNRQHLNGHLDAELERVAALGEKLAVVAIDLNGFRAINDRHGHGV
                     GDRMLKAVAARLSDALRDGEFAARFGADEFAAVKSYRDDGALDDFLTRLSAAVGAVTE
                     APDAQGRVLSLGGGGAIGAALYPQDAATRETLLNNANLAMWRARASHGDQPCFFDAGA
                     DEAARDRRALTHDLERALENGEFRLFYQVQQAVATGAVTGYEALIRWNHPQRGFVSPA
                     DFIPLAEESGAIVAIGEWVLRTACEEAARWDESLRIAVNLSPVQLSDERLIATVREAL
                     VVTGLSPSRLELEITETSIIEDRTRALHILRQIKALGVSIAIDDFGTGYASLDTLGAF
                     PFDKIKIDRSFLMQADASPQAHAIVRAILALGRSLGIPVLAEGVETQSQLDL"
BASE COUNT          379 a         1026 c          959 g          415 t
ORIGIN      
        1 ggagaaggtt gcgacggcgg tgcagaaaga ccgtatccga cagggcctga gtttatgttc
       61 cgagtacttt actgcctgcg cgatcaccac gactggaaac tggtcgcgct ggccgccatc
      121 gtctgcctga tttccagcgc cgtcaccgtc atgatgatcg cccgcgccgg gctgacgcgc
      181 gaagcggcgc gcaaggaggt ccggcgcagc tacgccggct gggtggccgc gtccggcgcg
      241 gcggcgggct tcgccatctg ggcgacgcac ttcatcgcca tgctggccta caatcccggc
      301 gtgccgatga ccttttcgcc cggcctgacc ctgctgtcgc tgctggtcgc cgtcgccctg
      361 acggcgggcg gcatcgagat cggcgtctcc agggcgtttc gcggctccgg cctgaccggc
      421 ggcgcggtga tcggcgcggg catcgcctcg atgcacttca tcggcatggc cgcgctcgac
      481 gtgccggggc ggctcgtctg ggcgcccgac ctcgtcttcg tctccatcgc ggcgggcatg
      541 gcgctgtcgg cggcggccat gtggtcggtc aaccgcttcc gccgccatcc ggtctggcgc
      601 gcggcgccgc tgctgaccct ggccatcatc tgccaccatt tcgtcgccat gggcgcggtc
      661 acggtgctgc ccgacccggc gcgcggcgtc gcggggctga gcctgtcgcc gaccgccatg
      721 gccgccgccc tgttctgcgc cgccgccgcc gtcatggccg tctgcatgtt cgcggtgatc
      781 tggggccagc gcttcgacgc gatgcgcgcc gacaccgacc ggcgcttccg ggtgctgctg
      841 gaggggctgg aggacacctt gctgttcctg ctggaccgcg aggggcgcgt gctggactgg
      901 aacaacggct ccgagcgcaa caaccgctat ccggtcggcg agatcgtcgg caagatctac
      961 ggcgacgtct gcgcgctgga cgcccagttc ccgctgcgtt accgcgcggc gctggccgcc
     1021 gccgccgccg agggccgcgc cgagctggag gcgctgattc aggacgccga cggccaccgc
     1081 ttctggggcc agaccaccct gcgggcgctg cgcggcgacg acggcgagct gctcggcttc
     1141 gcctcgatca cgcaggacgt caccgcccgc aaggccgatc aggaccgcag cttcgaggcg
     1201 acgcgcaatc tcgacgccgc cctgtcgcac atgtcgcagg gcctgctttt gttcgaccgc
     1261 aacgaaaagc tggtgctggc caacaagcgt tttctcgaca tctacggcct ggacccggcg
     1321 cgggtcacgc ccggcgtgtc gttccgcgac ctgctcgtca tgctgatggc cacgcgcgac
     1381 ggcgcggcgc cgggcccgga cgagatcgag gcctggcacg cgcgcaaccg cgccctgatc
     1441 gccgcgcccg gcggcgggga catggtgtcg gacttcttcg cccaccgcac catgtcggtg
     1501 tcgcaccgcc cgatgccgga cggcggctgg gtgacgacct tcgaggacat caccgagcgg
     1561 cgcaaggccg aggcgcgcat cgcccacatg gcccgccacg acggcctgac cggcctgccc
     1621 aaccgtcagc acctgaacgg ccatctcgac gccgagctgg agcgcgtggc ggctttgggc
     1681 gagaagctgg ccgtggtcgc catcgacctc aacggcttcc gcgccatcaa cgaccgccac
     1741 ggccacggcg tcggcgaccg gatgctcaag gcggtggccg cgcgcctgtc ggatgcgctg
     1801 cgcgacgggg aattcgccgc ccgcttcggc gccgacgagt tcgccgccgt caaatcctat
     1861 cgcgacgacg gcgcgctcga cgacttcctg acccgcctga gcgccgccgt cggcgcggtc
     1921 accgaggcgc cggacgcgca aggccgcgtg ctgtcgctgg gcggcggcgg cgccatcggc
     1981 gcggcgctct acccgcagga cgccgcgacg cgcgaaaccc tgctgaacaa cgccaacctg
     2041 gccatgtggc gcgcgcgcgc cagccacggc gaccagccgt gcttcttcga cgccggcgcg
     2101 gacgaggccg cgcgcgaccg ccgcgccctg acgcacgatc tggagcgcgc gctggaaaac
     2161 ggcgaattcc gcctgttcta tcaggtgcag caggccgtcg cgaccggcgc ggtcaccggc
     2221 tacgaggcgc tgatccgctg gaaccacccg cagcgcggct tcgtctcgcc cgccgacttc
     2281 atcccgctgg ccgaggaaag cggcgccatc gtcgccatcg gcgaatgggt gctgcgcacc
     2341 gcctgcgagg aggcggcgcg ctgggacgaa tccttgcgca tcgcggtcaa cctgtcgccg
     2401 gtgcagctca gcgacgagcg gctgatcgcg acggtgcgcg aggcgctggt cgtcaccggc
     2461 ctgtcgccgt cgcggctgga gctggagatc accgagacct cgatcatcga ggaccgcacg
     2521 cgcgccctac acatcctgcg ccagatcaag gcgctgggcg tcagcatcgc catcgatgat
     2581 ttcggcaccg gctacgcctc gctcgacacg ctgggcgcct tcccgttcga caagatcaag
     2641 atcgaccgct ccttcctgat gcaggccgac gccagcccgc aggcccacgc catcgtgcgc
     2701 gccatcctgg cgctgggccg aagcctcggc atcccggtgc tggccgaggg cgtggagacg
     2761 cagagccaac tcgaccttt
//
LOCUS       DASVLL010000444         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_137661,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000444 DASVLL010000000
VERSION     DASVLL010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137661"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /gene="acpA"
                     /locus_tag="VF402_07365"
     CDS             <1..1364
                     /gene="acpA"
                     /locus_tag="VF402_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEX7800152.1"
                     /translation="LNVLPKIWGGLVKTEQVVNHTSYKVGEDDIQGLPNAPFALATPN
                     GDPLPQGVVTRDLIHAFYQNQMQINGGRNDGFAAWGDSGARVMGHYGDNNVNLRLWRI
                     AQDYTLCHNFFMGAFGGSFLNHQYLVAAQPPYYPHADQSPAKSLIATVRGDPKGYDLQ
                     PAPDMPSSCMDGPARFGPSALSPDFHVVNTMSPPYAPTMTLDAKNPLYGDPGNSRTLP
                     PQVHATIGDRLSDKGIDWAWYSGGWGDALARAAGARKAETDVFPSRPNFQDHHQPLNY
                     FKSFAPGTQARAAHLRDAGTDETARTNRFMADIEAGKLPPVAFYKPQGNLNMHAGYSD
                     VDSGDRHIATIIDALQNGPQWANMLIVVTFDENGGWWDHVAPPKGDRWGPGTRVPALI
                     VSPFARRGFVDHTVYDTGSISRFLVRRFGLETLPGLEMRDEGMMKQEGFRPGDLTGAL
                     RFS"
     gene            1468..2079
                     /locus_tag="VF402_07370"
     CDS             1468..2079
                     /locus_tag="VF402_07370"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX7800153.1"
                     /translation="MFKFTPSRMIAVSVCGALAAGAFSLSACNKTQQAQTSEATSSAM
                     SDAGAMMSDTGSMIADAVTPDTPQDFANKAAVANTFELEESRLALKTSKNADVLAFAK
                     KMIADHTKAGKALDAAVAKSGKVSAPSATLDDAHQSKLDDLKKKTGDDFDKAYISDQK
                     DAHSEAVSLFDHYSKNGDDAALKDFAAATLPVLQSHKDMIDKM"
     gene            2201..2461
                     /locus_tag="VF402_07375"
     CDS             2201..2461
                     /locus_tag="VF402_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800154.1"
                     /translation="MAGVVYHLIDNNTGEEVSASDGFAFAVPPVPDHVIHDEVLRERY
                     GGPAIVDRVETLPDGGVNVYIDGREELTNNDAIDADQAYRRS"
     gene            complement(2446..3135)
                     /locus_tag="VF402_07380"
     CDS             complement(2446..3135)
                     /locus_tag="VF402_07380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7800155.1"
                     /translation="MSRTPSEANRAGYDRWSKLYDDTVNSTVAMDERAFPPVWAHVRG
                     RDVLEIGCGTGRHTLRLAAAGNRVTGMDLSPGMLAEARRKLADFPEVRLLQADILRDE
                     PEPEGFDVVLTALVLEHIADPAAFFRRAARALRPGGAFYLSEIHPQRIAGGAQANFID
                     EDGEAVRLTSFAHSAEAIEAAAARAGLRLDRARDIAGDAALAERNAGWARHIGRPMLR
                     VWSFNKPQERR"
     gene            3170..3784
                     /locus_tag="VF402_07385"
     CDS             3170..3784
                     /locus_tag="VF402_07385"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7800156.1"
                     /translation="MFAILRMGRRATTHRLIIASGARVQLARGALACQPARMTLYYDH
                     IETPFGATAAVVDDDGALVEFTFLNGVMDNLYAHDRGATREAARIGAVAAQVRDFFAR
                     RRTGFDLPLRPRGTDFQRRVWTALCDIPFGETISYQELARRVGDARAMRAVGAANGRN
                     PIALIVPCHRVIGKSGKLTGYGGGLPLKRALLDFEAQAAAPALF"
     gene            3841..>4242
                     /locus_tag="VF402_07390"
     CDS             3841..>4242
                     /locus_tag="VF402_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7800157.1"
                     /translation="MSKSSLPLILLAPLLLLSACHRAAPAAADNDGGADMTMADPVEY
                     TDAQKTAILATFPAPYNAADLSEGEKEFNKCRACHTITPDKMNMTGPHLYGVFGRKSG
                     TAPGYPFTEAMTNHDVVWGFDTLDTYLTSPQT"
BASE COUNT          743 a         1490 c         1358 g          651 t
ORIGIN      
        1 tattgaacgt cctgccgaaa atctggggcg ggctggtcaa gaccgaacag gtggtcaatc
       61 atacgagcta caaggtcgga gaggacgaca ttcagggcct gcccaacgcg ccgttcgcgc
      121 tggcgacacc gaacggcgat ccgctgccgc agggcgtggt gacgcgcgac ctgatccacg
      181 ccttttatca gaaccagatg cagatcaacg gcggccggaa cgacggcttc gccgcctggg
      241 gcgattccgg cgcgcgggtc atgggccatt acggcgacaa caacgtcaat ctgcgcctgt
      301 ggcggatcgc gcaggactat accctgtgcc acaacttctt catgggcgcg ttcggcggct
      361 cgttcctgaa ccaccagtac ctcgtcgccg cgcagccgcc ctattatccg cacgccgatc
      421 aaagcccggc gaaaagcctg atcgccacgg tgcgcggcga tccgaagggc tacgacctgc
      481 aaccggcgcc ggacatgccg tcaagctgca tggacggacc ggcgcgcttc ggccccagcg
      541 ccctgtcgcc ggacttccac gtcgtcaaca cgatgagccc gccctacgcg ccgaccatga
      601 cgctggacgc gaaaaacccg ctgtacggcg atccgggcaa ttcgcgcacc ctgccgccgc
      661 aggtccacgc caccatcggc gaccggctga gcgacaaggg gatcgactgg gcgtggtaca
      721 gcggcggctg gggcgacgcc ttggcgcgcg cggccggggc gcggaaggcc gaaacggacg
      781 tcttcccgtc gcggcccaat tttcaggacc accaccagcc gctcaactat ttcaagagct
      841 tcgcgcccgg cacgcaggcg cgcgcggcgc acctgcgcga cgccggaacc gacgaaacgg
      901 cgcgcaccaa ccgcttcatg gccgacatcg aggccgggaa gctgccgccg gtggcgtttt
      961 acaagccgca gggcaatctc aacatgcacg ccggttattc cgacgtcgac agcggcgacc
     1021 ggcacatcgc cacgatcatc gacgcgctgc aaaacgggcc gcagtgggcg aatatgctga
     1081 tcgtcgtgac cttcgatgaa aacggcggct ggtgggacca tgtcgcgccg ccgaaaggcg
     1141 accgctgggg gccgggcacg cgcgtgccgg cgctgatcgt ctcgccgttc gccaggcgcg
     1201 gcttcgtcga tcacacggtc tatgacaccg gctcgatctc gcgcttcctg gtgcggcgtt
     1261 tcgggctgga gaccctgccc ggccttgaga tgcgcgacga aggcatgatg aaacaggagg
     1321 gtttcaggcc cggcgacctc accggcgcgc tgcggttttc gtagtccgtc cctaccgtct
     1381 ctttgcaatc gcggccccgt cacaaagcgc gattttattt gacggcgcgt aggatgcccg
     1441 gcgttcacaa cctcgtgagg atacgccatg ttcaagttca ccccttcccg tatgatcgcc
     1501 gtctcggtct gcggcgcgct ggccgccggc gcgttcagcc tgtccgcctg caacaagacc
     1561 cagcaggcgc agacctcgga agccaccagc agcgccatgt ccgacgccgg ggccatgatg
     1621 tccgacaccg gctcgatgat cgccgacgcg gtcacgccgg atacgccgca ggacttcgcc
     1681 aacaaggcgg cggtcgccaa tacgttcgag cttgaggaaa gccgcctggc gctcaagacc
     1741 tcgaagaacg ccgacgtcct ggccttcgcg aagaagatga tcgccgacca caccaaggcg
     1801 ggcaaggcgc tcgacgccgc cgtggccaag tccggcaagg tgtccgcccc gtccgccacg
     1861 ctggacgacg cgcatcagtc caagctggac gacctgaaga agaagaccgg cgacgatttc
     1921 gacaaggctt acatcagcga ccagaaggac gcgcacagcg aagcggtcag cctgttcgac
     1981 cattacagca agaacggcga cgacgcggcg ctcaaggact tcgccgccgc caccctgccg
     2041 gtgctgcaaa gccacaagga catgatcgac aagatgtgac cggtccggaa ggcggcccgt
     2101 cccggtcgcc ttccgcccgc ggcgcatgac cgcattgccc gcttatgtct tttgcgtttc
     2161 cctgtcccgg cagaggacaa aacccggcag gaggcaagcc atggcaggcg ttgtttatca
     2221 tctcatcgac aacaataccg gcgaggaggt gtccgcatcc gacggcttcg ccttcgccgt
     2281 tccgccggtc cccgatcacg tcatccacga cgaggtcttg cgcgaacgct atggcggtcc
     2341 ggccatcgtc gaccgcgtgg agaccctgcc ggacggcggc gtcaacgtct atatcgacgg
     2401 gcgcgaggaa ttgaccaata acgacgccat cgacgccgat caggcttatc gccgttcctg
     2461 aggcttgtta aacgaccaga cgcgcagcat cggccgtccg atatggcgcg cccatccggc
     2521 gttgcgttcc gccagcgccg cgtcgccagc gatgtcgcgc gcccggtcca gccgcaatcc
     2581 cgcgcgcgcc gccgcggcct cgatcgcctc ggcgctgtgg gcgaagctgg tcaggcgcac
     2641 ggcctcgccg tcctcatcga tgaaattggc ctgcgcgccg cccgcgatgc gctgcggatg
     2701 gatttccgac aggtagaaag cgccgccggg ccgcaaggcc cgcgccgcgc gcctaaagaa
     2761 ggcggcggga tcggcgatat gctccagcac cagcgccgtc agcacgacgt cgaagccctc
     2821 cggctccggt tcgtcgcgca ggatatcggc ctgcaacagg cgcacctccg ggaagtcggc
     2881 cagtttacgc cgcgcctcgg ccagcatgcc cggcgacagg tccatcccgg tcacgcggtt
     2941 gcccgccgcc gccagccgca gcgtatgccg ccccgtgccg cagccgatct ccagcacgtc
     3001 gcggccgcgc acatgggccc agaccggcgg gaaggcccgt tcgtccatcg cgacggtcga
     3061 gttgacagtg tcgtcgtaca gcttcgacca gcggtcgtaa ccggcgcggt tggcttcgga
     3121 gggggtgcgg gacatggcga tggctcctga aacaaaggct aggagcgtat tgttcgccat
     3181 ccttcggatg ggccgccgcg ccacgacgca ccggcttata atcgcgtccg gcgcccgtgt
     3241 ccagcttgcg cgcggcgcgc tcgcgtgtca gcctgcgcgc atgacgctct attacgacca
     3301 tatcgagacg ccgttcggcg caaccgcggc cgtcgtcgac gacgacggcg cgctggtcga
     3361 attcaccttc ctgaacggcg tcatggacaa tctttatgcc cacgatcggg gcgcgacgcg
     3421 cgaggcggcg cgaatcggcg ccgtcgccgc gcaggtgcgg gatttcttcg cgcgccgccg
     3481 gaccggcttc gacctgccgc tgcggccgcg cggcacggat tttcagcgcc gcgtctggac
     3541 cgcgttgtgc gatatcccgt tcggcgagac gatctcgtat caggaactgg cccggcgcgt
     3601 cggcgacgcc agggccatgc gcgcggtggg cgcggccaat ggtcgcaacc cgatcgcgct
     3661 gatcgtgccg tgccaccgcg tcatcggcaa gtccggcaag ctgaccggct atggcggcgg
     3721 cctgccgctc aagcgcgccc tgctcgattt cgaggcgcag gccgccgccc cggcgctttt
     3781 ctgactttgc agcccgcagc gggcggccta caagacgggc gtcatccgtg gagtcgcccc
     3841 atgtcgaaat cgtccttgcc gctgatcctg ctcgcgcccc tgctgctgct gagcgcctgc
     3901 caccgcgccg cgcccgccgc cgcggacaat gacggcggcg cggacatgac catggccgat
     3961 ccggtggaat acaccgacgc gcagaagacc gcgatcctgg cgaccttccc ggcgccctac
     4021 aacgccgccg acctgagcga gggcgaaaag gagttcaaca agtgccgcgc gtgccatacg
     4081 atcacgccgg acaaaatgaa catgaccggt ccgcacctgt acggcgtgtt cggccgcaaa
     4141 tcgggcacgg cgccgggcta tccgttcacc gaggcgatga cgaatcatga cgtcgtctgg
     4201 ggcttcgaca cgctggacac gtacctgacc tcgccgcaga cc
//
LOCUS       DASVLL010000445         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_189870,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000445 DASVLL010000000
VERSION     DASVLL010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189870"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VF402_07395"
     CDS             <1..445
                     /locus_tag="VF402_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800158.1"
                     /translation="PTGSIRAKPVRPVVFGIAGGALAAAVLAGCVTGGEAQPGGRSLA
                     VGQCLSQPLDETRVLDDHTLLALDRSGRGALFHMASACLRVDEAVIMRYFSGGPVCGP
                     LDVDIAGSDGGMMAPTHCLVDSVTPLDKVEARRMLDGPERRKLHK"
     gene            complement(436..1293)
                     /locus_tag="VF402_07400"
     CDS             complement(436..1293)
                     /locus_tag="VF402_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800159.1"
                     /translation="MLPETSTKTVLVLEDSRTQARIVGALFERCGLKVDFTFTMDEAL
                     HALRRRSYYLILLDVFVADENVLDSIDRFQKLAAGAPVAVMTAGRRGKPTAASHALNA
                     ARRACVDYLLPKPFDYKDIMQICGEIEDGRRHRKPMRRVLIIDDDAYTRAFVRDIIEE
                     AGFYAAESASVEEAIMRLDITRIDAVIIDIVMPGISGMTGIKIIKATWPSVPIIAMTG
                     YLKDTDYLESALLKGAAHALPKPFGKLQLTGVLEGCLAASGPRDGSPEDNTEEHPDDQ
                     GVGDGPLYL"
     gene            complement(1321..>1571)
                     /locus_tag="VF402_07405"
     CDS             complement(1321..>1571)
                     /locus_tag="VF402_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800160.1"
                     /translation="QACVGHWNFAQRRRAPVLSHARLIRDMSAAAFAEFERTLIRERT
                     LTGPRRPRASQICVGKRAPRRIFRIASAVRPWSQRYNK"
BASE COUNT          238 a          488 c          567 g          278 t
ORIGIN      
        1 gccgaccggc tcgatccggg ctaagcccgt gcgcccggtt gtcttcggca tcgccggcgg
       61 cgcgctggcg gcggctgtgc tggccggttg cgtgacgggc ggcgaagcgc agccgggcgg
      121 gcgctcgctg gcggtcggtc agtgcctgtc gcagccgctg gacgagacgc gcgtgctgga
      181 tgaccatacg ctgctggcgc tcgaccgctc cgggcgcggg gcgctgttcc acatggcgtc
      241 ggcgtgtctg cgggtcgatg aagcggtgat catgcgctat ttcagcggcg gtccggtctg
      301 cggcccgctc gacgtcgata tcgccgggtc cgatggcggc atgatggcgc ccacgcattg
      361 tctggtcgac agcgtgacgc cgctggacaa ggtcgaggcg cggcggatgc tggacgggcc
      421 ggagcgccgg aagcttcaca aatagagcgg tccgtcgccg acgccctgat cgtcgggatg
      481 ttcttccgta ttgtcctccg gcgatccgtc ccgcggcccc gacgcggcga ggcagccttc
      541 cagcacgccg gtcagttgca gcttgccgaa cggcttgggc agggcgtgcg ccgcgccctt
      601 gagcaacgcg ctttccagat agtcggtgtc cttcagatag ccggtcatgg cgatgatcgg
      661 cacgctcggc cacgtcgcct tgatgatctt gatgccggtc atgccgctga tgcccggcat
      721 gacgatgtcg atgatcacgg cgtcgatgcg cgtgatgtcc agccgcatga tcgcttcctc
      781 gaccgaggcg ctctcggcgg cgtagaagcc ggcctcctcg atgatgtcgc gcacgaaggc
      841 gcgcgtatag gcgtcgtcgt cgatgatcag cacgcggcgc atcggcttgc ggtggcggcg
      901 gccgtcctcg atctcgccgc agatctgcat gatgtccttg tagtcgaacg gcttgggcag
      961 cagatagtcg acgcaggcgc gccgcgcggc gttgagcgcg tggctggcgg cggtcggctt
     1021 gccgcgccgc ccggcggtca tcacggccac cggcgcgccc gcggccagct tctgaaagcg
     1081 gtcgatgctg tccagaacgt tctcgtcggc gacgaagacg tcgagcagga tcaggtaata
     1141 gctgcgccgc cgcagcgcgt gcagcgcctc gtccatggtg aaggtgaaat cgaccttgag
     1201 gccgcaacgc tcgaacaagg cgccgacgat acgcgcctgg gtgcgggaat cttccagaac
     1261 gaggacggtt ttggtggaag tctctggaag cattggcaga acgactgtta tcacgagatg
     1321 ttacttattg taacgttgtg accacggccg gacggcggac gcaatacgga aaatgcggcg
     1381 cggcgcccgc ttacccacac aaatctgtga agcgcgcggg cgtcggggcc cggtcagcgt
     1441 gcgttcgcgg attagggtgc gctcgaattc ggcgaacgcg gcggcggaca tatcccgaat
     1501 aaggcgggcg tgcgataata ccggcgcgcg gcggcgctgc gcgaagttcc agtgtccgac
     1561 gcaggcttgc c
//
LOCUS       DASVLL010000446         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_351189,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000446 DASVLL010000000
VERSION     DASVLL010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351189"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VF402_07410"
     CDS             <1..528
                     /locus_tag="VF402_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7800161.1"
                     /translation="IDQGTIVCDEEEIVEKHIVSGVAFSRDEAKITLLGLPNKIGVSA
                     AVFSKLAAANINVDMIVQSEKRSGDYANQIFTVGRRDAAQAVQIMQDAREEIGFDDIR
                     LDDTVSKVSIVGVGMRPHTGVAETMFRALSEKGINIQVISTSEIKISVLIDEAYTELA
                     VRALHAAYGLDKIGV"
     gene            754..2439
                     /locus_tag="VF402_07415"
     CDS             754..2439
                     /locus_tag="VF402_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX7800162.1"
                     /translation="MAFKHLPMLGKILCLLGLLTAVVVIAVGYTTSRMKAISGDFGRV
                     IEGPGVAAVDMERASKNIQRAQTALYKLVSFSDPEDIAAAEGELKQAQSSFSKETNDA
                     LKALPKATQRIEGIQASYNAAVDQTCAQMRALAERGDDADALRQMRTACDPAITQLEK
                     TMAQVVDDTIADNVAASTAAGADTRRTIGVTWGGLTVGLVLVLGLAVLVTRKGVVAPL
                     TTLNATMTQMDKGDLDTPPPGRERRDELGAMARTLETFRQGLAEGVRLRAEAEAARAA
                     ELAKAQRERGIVDAFQGKMVNLASSFVRSSGEVSEAAQSLSATAEETSRQAQVVTGAA
                     EEAAGSVQTAAAATEEMAASVQEINAQVVRAAEVARDAADEIGGTEVEIRGLSEAAAG
                     IEAVVGLINDIAAQTNLLALNATIEAARAGDAGKGFAVVASEVKALAGQTAHATQDIS
                     RRVELIQEATRRTVQSIERIVATIADMRDISASIASAVEQQGAATHEIAGNASRAADG
                     AQTVTENIFGVGRAAEMTGAASTQLMGLSGSLSEQAGDLQDEVRAFVSALQAA"
     gene            2655..>3676
                     /locus_tag="VF402_07420"
     CDS             2655..>3676
                     /locus_tag="VF402_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX7800163.1"
                     /translation="MLGKVFTLLLLMAAVTVCAVGFTTSRMKSIDHDYSTVISGPGVA
                     VVNMERAARQVVKSETAFYKLVSSSDQADLSVADDDLKTAETTFATETRSAIRAAPNE
                     TGRIQGIEASYDTVVDQTCAQTQKLALADDDAAALKQMTASCEPAFSQVEQTMSKVVD
                     DTLADNAAASAAATKFTRHTEHISWAGALAGLGLVLVAAFFMTRLTIVRPLQDLTGTM
                     GSLSSGQLRVEVPGRERGDELGAMARSTDVFRQGLEAAEALRLEAADQERRSAERMKA
                     ERHAIADSFQSKMGALASDFVRSSSEVSDAAQSLAATAEETSRQAQVVSGAAEEAAVN
                     VQTIAAA"
BASE COUNT          668 a         1197 c         1259 g          552 t
ORIGIN      
        1 atcgaccaag gcaccatcgt gtgcgacgag gaagaaatcg tggaaaagca tatcgtttcc
       61 ggcgtggcgt tcagccgtga tgaagccaag atcaccttgc tgggcctgcc caacaagatc
      121 ggcgtgtcgg ccgcggtctt ctccaagctg gccgccgcca acatcaatgt cgacatgatc
      181 gtgcagtcgg aaaagcgctc cggcgactac gccaaccaga tcttcaccgt cggccgccgc
      241 gacgccgcgc aggccgtgca gatcatgcag gacgcccgcg aagagatcgg cttcgacgat
      301 atccgcctcg acgatacggt gtccaaggtg tcgatcgtcg gcgtcggcat gcgcccgcac
      361 accggcgtgg ccgagaccat gttccgcgcc ctgtcggaaa agggcatcaa tattcaggtc
      421 atctcgacct cggaaatcaa gatttccgtg ctgatcgacg aagcctatac cgaactggcc
      481 gtgcgcgccc tgcacgccgc ctatggcctc gacaagatcg gcgtctaggt agagcgtttc
      541 cccaaaaaac agctccgctt tttggataag gaagagcgat aaaacaaaag cctggagcgc
      601 cgatccgatt ccatcggatc ggcgctccag gcgggcctga cggcggcttt tgttgcaacg
      661 gcgccatcat tgcgccgctc aggttgcgat taggcaaaat ttaaccacag ttcagcacat
      721 cctgtcgcat cggattcgcg aagggtttgc gtcatggcgt tcaagcacct gcctatgctg
      781 ggcaagattc tttgcctgct cggtctgttg acggcggtgg tggtgatcgc cgtcggttac
      841 acgacctcgc gcatgaaggc catcagcggc gatttcggca gggtcatcga agggccgggc
      901 gtcgcggcgg tcgacatgga gcgcgccagc aagaacatcc agcgcgccca gaccgcgctc
      961 tataagctgg tgtcgttcag cgacccggaa gacatcgccg ccgccgaggg cgagctgaaa
     1021 caggcgcaaa gctcgttctc gaaggagacg aacgacgcct tgaaggcgct gccgaaggcg
     1081 acgcagcgca tcgagggcat tcaggccagc tacaatgccg ccgtcgatca gacctgcgcg
     1141 cagatgcgcg ctctggccga acgcggcgac gacgcggacg cgctgcgcca gatgcgcacc
     1201 gcctgcgatc cggcgatcac gcaactggaa aagacgatgg cgcaggtggt cgacgacacc
     1261 atcgccgaca atgtcgccgc gtccaccgcc gccggggcgg atacgcgccg caccatcggc
     1321 gtgacctggg gcgggctgac ggtcgggctg gttctggtgc tggggctggc cgtgctggtg
     1381 acgcgcaagg gcgtggtcgc gccgctgacc acgctgaacg cgacgatgac gcagatggac
     1441 aagggcgatc tggacacgcc gccgccggga cgcgagcgcc gcgacgaact gggcgcgatg
     1501 gcgcgcacgc tggagacgtt ccggcagggg ctggccgagg gcgttcgctt acgcgccgag
     1561 gccgaggccg cgcgcgccgc cgaactagcc aaggcgcagc gtgagcgcgg catcgtcgac
     1621 gcgttccagg gcaagatggt caatctggcc tcgtcgttcg tgcgctcgtc cggcgaggtg
     1681 tccgaggcgg cgcagagcct gtcggctacc gccgaggaaa ccagccgtca ggcgcaggtc
     1741 gtcaccggcg ccgccgagga ggccgcgggc agcgtgcaga ccgccgccgc cgcgaccgag
     1801 gaaatggccg cttcggtgca ggagatcaac gcgcaggtgg tgcgcgccgc cgaggtggcg
     1861 cgcgacgccg ccgacgagat cggcggaacc gaggtcgaga ttcgcggcct gtccgaagcc
     1921 gccgccggca tcgaggccgt ggtcgggctg atcaacgaca tcgccgcgca gaccaatctc
     1981 ctggcgctca acgccaccat cgaggcggcg cgggcgggcg acgcgggcaa gggcttcgcg
     2041 gtcgtggcgt ccgaggtcaa ggcgctggcc gggcagaccg cccacgccac gcaggacatc
     2101 agccgccgcg tcgagctgat tcaggaggcg acgcgccgca cggtgcagtc gatcgagcgc
     2161 atcgtcgcca ccatcgccga catgcgcgac atctcggcca gcatcgcctc ggcggtcgag
     2221 cagcagggcg cggccacgca cgagatcgcg ggcaatgcgt cgcgcgcggc ggacggcgcc
     2281 cagaccgtga ccgaaaacat cttcggcgtc gggcgcgcgg cggaaatgac cggcgcggcc
     2341 tcgacgcaac tgatgggcct gtcgggcagc ctgagcgagc aggcgggcga tttgcaggac
     2401 gaggtgcgcg ccttcgtgag cgccttgcag gcggcctgag ggcgcgccgg atcgattgct
     2461 tttgcggact cgcttctgag ttaagacgcg gcgttaaatt cgccttaacc gtgaatcgtt
     2521 aacgtccgac taggcgccgc atgtgtcggg ggcgagacca gtatcttctc gccgatgtct
     2581 tcccgtcatg ccggtgaagc gcgtatccgg gaggcggctc gatgtcgaaa tttctgtctt
     2641 tcaaaaatat gcccatgctg ggcaaggtgt tcacgctgct gctgctgatg gcggccgtga
     2701 ccgtgtgcgc ggtggggttc accacgtcgc gcatgaaatc catcgaccat gactacagca
     2761 cggtcatctc cggtcccggc gtcgccgtcg tcaatatgga gcgcgccgcc cgtcaggtcg
     2821 tgaagtcgga gacggccttc tacaaactgg tctcctccag cgatcaggcc gatctcagcg
     2881 tcgccgacga cgatctgaaa accgccgaga cgacgttcgc gaccgaaacg cgctcggcga
     2941 tcagggcggc gccgaacgag accggacgca ttcagggcat cgaggccagc tacgatacgg
     3001 tggtcgacca gacctgcgcg cagacgcaga agctggccct ggccgatgac gacgccgccg
     3061 cgctgaaaca gatgaccgcg tcgtgcgaac cggccttttc gcaggtcgag cagaccatga
     3121 gcaaggtggt cgacgacacg ctggccgaca acgccgccgc ctcggccgcc gcgacgaaat
     3181 tcacgcgcca caccgaacac atcagctggg ccggagcgct ggccgggctg gggctggtgc
     3241 tggtcgcggc cttcttcatg acgcgcctga ccatcgtgcg gccgttgcag gatctgaccg
     3301 gcacgatggg cagcctgtcg tccgggcagt tgcgcgtcga ggtgccgggg cgcgagcgcg
     3361 gcgacgaact gggcgcgatg gcgcgctcga ccgacgtctt ccggcagggg ctggaggcgg
     3421 ccgaagcctt gcgcctcgaa gccgccgatc aggagcgccg cagcgccgag cgcatgaagg
     3481 ccgagcgcca cgccatcgcc gattccttcc agtcgaagat gggcgcgctg gccagcgatt
     3541 tcgtgcgctc gtcgtcggaa gtctcggacg ccgcgcaaag cctggccgcc accgccgagg
     3601 aaaccagccg tcaggcgcag gtcgtctccg gcgccgccga ggaagccgcc gtcaacgtcc
     3661 agaccatcgc cgccgc
//
LOCUS       DASVLL010000447         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_189990,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000447 DASVLL010000000
VERSION     DASVLL010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189990"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VF402_07425"
     CDS             complement(<1..214)
                     /locus_tag="VF402_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7800164.1"
                     /translation="MADFSIELSLTGLICGVDEAGRGPCAGPVSVAAVILDPERLPEG
                     VDASKALTETRRFELEPLIKRAAVAWS"
     gene            complement(242..799)
                     /locus_tag="VF402_07430"
     CDS             complement(242..799)
                     /locus_tag="VF402_07430"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX7800165.1"
                     /translation="MKSILGLMFLPLRRYADFHGRSRRSEYWLWVLFKFLLGAALVLL
                     AGALGLSGHVGGVGVVALLRMILFFAIVIPDTAVAARRMHDIDRSGWWILMPAAVGAA
                     ASLLCLLVAGGSLMAEVQTLVAVSQTNDPELFLSGLTALIVPMIWVTGPILLAKIVTL
                     VFRCTPGTHGGNRFGPDPKAHGGLA"
     gene            complement(841..1431)
                     /locus_tag="VF402_07435"
     CDS             complement(841..1431)
                     /locus_tag="VF402_07435"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7800166.1"
                     /translation="METKTRCHWPGQDPLYVAYHDEEWGAPEYDPRALWEKLVLDGFQ
                     AGLSWITILRKREALRAAFAGFDPDKVARFNAADIERLMNDPGIIRSKAKILSAIDSA
                     RITLDLREKGVSFTDLIWQAQDHKPLHNHFEDRTQVPAETPASRELAKLLKSHGYKFC
                     GPVIVYAFMQAVGMVNDHLVTCFRHDELIAAASKPA"
     gene            complement(1421..2248)
                     /locus_tag="VF402_07440"
     CDS             complement(1421..2248)
                     /locus_tag="VF402_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX7800167.1"
                     /translation="MSVTTLAHRGLLSVSGPDWAGFLKGLCTAHIDDLAAEAGRGDLH
                     NLHYGAFLRPQGRMIGDCLLHAAAADEIWLDVPLDVRDELFAKLNMYRLRARVAIAKR
                     DEGVAVAMNALPGGFMPDPRSAVTGGVYGRAYGKDGGGGGWRAFRFALGLAEGGEDFG
                     ADDLYAIDANLDALRAIDFHKGCYVGQELTSRMKRRGQIKNRVLPLRHEGRLNKGAEV
                     LVGERKIGEVLSGGDGASLGLMRLDRLAPDAVCDGVPVAVAIPGWLQDLIPETTDGD"
BASE COUNT          413 a          811 c          803 g          392 t
ORIGIN      
        1 ccgaccacgc gacggcggcc cgtttgatca gcggctccag ctcgaaacgg cgcgtctcgg
       61 tcagggcctt ggaagcgtcg acgccttcgg gcaggcgctc cggatcgagg atgacggcgg
      121 ccacgctgac cggaccggcg cacgggccgc gcccggcttc gtcgacgccg cagatcaggc
      181 ccgtcaggct cagttcgatg ctgaaatccg ccatgcgctg cctttacgcc gattccgccg
      241 ctcaggcgag gccgccatgc gccttcgggt cgggaccgaa gcggttgccg ccgtgcgtcc
      301 ccggcgtgca gcgaaagacc agggtgacga tcttggccag caggatcggg ccggtgaccc
      361 agatcatcgg cacgatcagc gcggtcaggc cggacaggaa cagttcgggg tcgttggttt
      421 gcgacaccgc gaccagggtc tgcacctcgg ccatcaggct gccgcccgcg accagcaggc
      481 agagcagcga cgccgccgcg ccgaccgccg cgggcatcag aatccaccag ccggagcggt
      541 cgatatcgtg catccgccgc gccgccaccg ccgtgtcggg aatgacgatg gcgaagaaca
      601 ggatcatgcg cagcagcgcg accacgccga cgccgccgac atggccgctc aggcccagcg
      661 ctccggccag cagcaccagc gcggcgccca gcaggaattt gaacagcacc cacagccagt
      721 attcgctgcg gcgcgaccgg ccgtgaaaat cggcgtagcg ccgcaacggc aggaacatca
      781 gtcccagaat cgatttcata cgcgcccccg cactggcttc cggctgaaag cgccgcctct
      841 ttacgccggt ttcgacgcgg cggcaatcag ttcgtcatgc cggaaacagg tgacgagatg
      901 gtcgttgacc atgccgaccg cctgcatgaa ggcatagacg atcaccgggc cgcagaattt
      961 atagccgtgc gatttcagca gcttggccag ttcgcgcgag gccggggttt cggcgggcac
     1021 ctgcgtcctg tcctcgaaat ggttgtggag cggtttatgg tcctgcgcct gccagatcag
     1081 gtcggtgaag ctgacgccct tttcgcgcaa gtcaagcgtg atgcgcgcgc tgtcgatggc
     1141 cgataaaatc ttggccttcg agcggatgat gcccggatcg ttcatcagcc gttcgatgtc
     1201 ggccgcgttg aagcgcgcca ccttgtcggg atcgaacccg gcgaaggccg cgcgcagggc
     1261 ttcgcgcttg cgcaggatgg tgatccacga cagcccggcc tgaaagccgt ccagcaccag
     1321 cttttcccac agggcgcgcg gatcgtattc gggcgcgccc cattcttcgt cgtgataggc
     1381 gacgtagagc gggtcttgtc ccggccagtg gcagcgcgtc ttagtctcca tccgtcgtct
     1441 ccgggatcag gtcttgcagc cagccgggga tcgcgaccgc gacgggaacg ccgtcgcaaa
     1501 ccgcgtccgg cgcgaggcgg tcgaggcgca tcaggcccag cgaggcgccg tcgccgccgc
     1561 ttaagacctc gccgattttg cgttcgccga caagcacttc ggcgcctttg ttcagacggc
     1621 cctcatggcg cagcggcagc acgcggttct tgatctggcc gcgccgcttc atgcgcgagg
     1681 tcagttcctg cccgacatag cagcccttgt ggaagtcgat ggcgcgcagc gcgtcgagat
     1741 tggcgtcgat ggcgtagagg tcgtcggcgc cgaaatcttc gccgccctcg gcgaggccga
     1801 gcgcgaaacg gaaggcgcgc cagccgccgc cgccgccgtc tttaccgtag gcgcggccat
     1861 aaaccccgcc ggtgacggcg ctgcgcggat cgggcatgaa gccgccgggc agggcgttca
     1921 tcgcgaccgc cacgccttcg tcgcgcttcg cgatcgcgac ccgggcgcgc aggcggtaca
     1981 tgttgagttt ggcgaacaac tcatcgcgca catcaagcgg cacgtcgagc cagatttcgt
     2041 cagcagcggc ggcgtgcagc agacaatccc cgatcatcct gccctgtggc cgcaaaaacg
     2101 ccccgtaatg caggttatga agatcgcccc ggccggcctc ggcggccagg tcgtcgatat
     2161 gggccgtgca cagacccttg agaaaaccgg cccagtcggg gccggagacg ctgagcagtc
     2221 cgcgatgggc gagggtggtt acagacatga ttttgatatg aaacgcattg gttttaaact
     2281 ctgacgcatg gcggtgtttc cgattctcgt tctggccttc tgcgacgtcg cgcgcgccct
     2341 gagcgtgagc ggcgcgatcg cgcggctgaa acaggaggcg ccggaggccc gcatcacgct
     2401 ggccaccacg cccgccgtc
//
LOCUS       DASVLL010000448         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_29080, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000448 DASVLL010000000
VERSION     DASVLL010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29080"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1922
                     /gene="uvrA"
                     /locus_tag="VF402_07445"
     CDS             <1..>1922
                     /gene="uvrA"
                     /locus_tag="VF402_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7800168.1"
                     /translation="KHDIDVVVDRIVVKGGIMARLADSLETALRLADGLAIAEYADKA
                     EGESEPQRLIFSEKFACPVSGFTIAEIEPRLFSFNNPFGACPACDGLGVKLAFDEGLV
                     IPDPAATLEGGAVAPWSKGPSPLYAQTLKALALHYGVALNTKWQALPDGVRDVILHGS
                     GSEKIKFVYEDGSRRYDVSKPFEGVIPNMERRWRETDSSWVREDLARYQSETPCEACG
                     GARLKPEALAVKIDARNIAEISALSIRHAHEWFDAIEARLSEKQMDIARRILKEIRDR
                     LRFLNDVGLDYLNLSRNSGTLSGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDN
                     DRLLDSLKGLRDLGNSVLVVEHDEDAILSADYVIDMGPAAGVHGGEIVAEGAPEAIKA
                     NPKSLTGQYLTGKREITIPEDRRPIDRKKMLKVVGARGNNLKNVTGEIPVGVMTCITG
                     VSGGGKSTFTLETLYKAAARRLNNASAHPALHDRIEGLEFFDKIVEIDQSPIGRTPRS
                     NPATYTGAFGPIRDWFAGLPEAKARGYGPGRFSFNVKGGRCEACQGDGVIKIEMHFLP
                     DVYVTCDVCKGKRYNRETLEIVFKGHSIADVLDMTVEEGAEAFKAVPSIRDKLKTLER
                     VGLGYIKIGQQATTLS"
BASE COUNT          363 a          643 c          624 g          292 t
ORIGIN      
        1 tcaagcatga tatcgacgtg gtggtcgacc gcatcgtggt caagggcggc atcatggcgc
       61 gtctggccga ttcgctggag accgcgctgc ggctggccga cgggctggcc atcgccgaat
      121 acgccgataa ggcggagggc gaaagcgagc cgcaacggct gatcttctcc gaaaaattcg
      181 cctgtcccgt ctccggcttc accatcgccg agatcgagcc gcgtctgttt tcgttcaaca
      241 atccgttcgg cgcctgcccg gcctgcgacg ggctgggcgt caagctggcc ttcgacgaag
      301 ggctggtcat acccgatccc gcggcgactc tggaaggcgg cgccgtcgcc ccatggtcga
      361 aaggcccctc gccgctctac gcccagacgc tcaaggcgct ggccctgcat tacggcgtgg
      421 cgctgaacac gaaatggcag gccctgccgg acggcgtgcg cgacgtgatc ctgcacggct
      481 ccggctcgga aaagatcaag ttcgtctatg aggacggctc gcgccgctac gacgtaagca
      541 agccgttcga gggcgtgatc ccgaacatgg agcgccgctg gcgcgagacg gattcgtcgt
      601 gggtgcgcga agacctcgcc cgctaccagt ccgaaacgcc gtgcgaagcc tgcggcggcg
      661 cgcgcctcaa gcccgaagcc ctggccgtta agatcgacgc ccgaaacatc gccgaaatct
      721 cggccctgtc gatccggcac gcgcacgaat ggttcgacgc catcgaggcc cgcctgagcg
      781 aaaagcagat ggacatcgcg cgccgcattc tgaaggagat ccgcgaccgg ctgcgcttcc
      841 tcaacgatgt cggcctcgac tatctcaacc tgtcgcgcaa ttccggcacg ctcagcggcg
      901 gcgagagcca gcgcatccgg ctggcctcgc agatcggctc cggcctgacc ggcgtgctgt
      961 acgtgctgga cgagccgtcc atcggcctgc atcagcgcga caacgaccgg ctgctggaca
     1021 gcctcaaggg cctgcgcgac ctcggcaatt cggtgctggt ggtcgaacat gacgaggacg
     1081 ccatcctgag cgccgattac gtcatcgaca tgggcccggc ggcgggcgtg catggcggcg
     1141 agatcgtggc ggaaggcgcg cccgaagcga tcaaggccaa cccgaaatcg ctgaccggcc
     1201 aatacctgac cggcaagcgc gaaatcacca tccccgaaga ccgccgtccc atcgaccgca
     1261 agaagatgct gaaagtcgtc ggcgcacgcg gcaacaacct gaaaaacgtc accggcgaga
     1321 tcccggtcgg cgtgatgacc tgcatcaccg gcgtgtccgg cggcggcaag tcgaccttca
     1381 cgctggagac gctgtacaag gcggcggcgc ggcggctcaa caacgcctcg gcgcacccgg
     1441 ctctgcacga caggatcgaa gggctggaat tcttcgacaa gatcgtcgag atcgaccagt
     1501 cgccgatcgg gcgcacgccg cgctccaacc cggccaccta taccggcgcc ttcggtccga
     1561 tccgcgactg gttcgcgggc ctgcccgaag ccaaggcgcg cggctacggg ccggggcgct
     1621 tctcgttcaa cgtcaagggc gggcgctgcg aggcgtgtca gggcgacggc gtgatcaaga
     1681 tcgagatgca tttcctgccc gacgtctatg tcacttgcga cgtctgcaag ggcaagcgct
     1741 ataaccgcga aacactggag atcgtgttca agggccactc catcgccgac gtgctggaca
     1801 tgacggtcga ggaaggcgcc gaggcgttca aggcggtgcc gtcgatccgc gacaagctca
     1861 agacgctgga gcgggtcggc cttggctaca tcaagatcgg ccagcaggcc accaccctgt
     1921 cc
//
LOCUS       DASVLL010000449         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_190059,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000449 DASVLL010000000
VERSION     DASVLL010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190059"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..540
                     /locus_tag="VF402_07450"
     CDS             37..540
                     /locus_tag="VF402_07450"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX7800169.1"
                     /translation="MPPIPPRKSSFPPVVDARTRLLICGSLPGERSLAAQRYYAHPQN
                     QFWRLMSSVLDQDLAALDYGARLAALRRAGVGLWDTVASARRLGSTDAAIRDHAPNDL
                     KALAATLPTLRAVGFNGATAAKLGTKALLGTPLALIALPSSSPLHTVGLEAKLPAWMV
                     LRAWVDA"
     gene            600..992
                     /locus_tag="VF402_07455"
     CDS             600..992
                     /locus_tag="VF402_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEX7800170.1"
                     /translation="MGINLNPFTWWTGATWGTMAGLLGKTRVGEDALGNVYYQGGRDT
                     AGQPRRWVIYDGANDASRIAPEWFSWLHHQVDDVPERALPAPRRWEKPAVPNMTGTAL
                     AYRPPGALEKGGRRAAATGDYEAWTPEG"
     gene            989..>1580
                     /locus_tag="VF402_07460"
     CDS             989..>1580
                     /locus_tag="VF402_07460"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEX7800171.1"
                     /translation="MTRRQLAVGAAIAVLIGLSGWVGYRWWDSRQAAEQAAQRPPSAL
                     DEQESASNVTMVQMPEGADPSYGATPMAERVAVLGLLNKRNGQTRDVTLKPGQATRVG
                     DVIIRLRACERTAPWEDQDLTGAFVQLDVKQFDNSWRRVFSGWLYKERPALNVVQHPI
                     YDVWPKSCAMTFPEGGPNSTSAGSAASTPAPSRSSAP"
BASE COUNT          236 a          579 c          550 g          215 t
ORIGIN      
        1 cttgcctgcg ccccccagcg cgaggaaacg gacgccatgc cgcccatccc gccgcgcaaa
       61 tcctccttcc caccggtcgt cgacgcgcgc acccgcctgc tgatctgcgg cagccttccg
      121 ggcgagcgca gcctggcggc gcagcgttat tacgcgcatc cgcagaacca gttctggcga
      181 ttgatgtcat cggtgctcga ccaggatctg gccgcgctgg actatggcgc gcggctggcg
      241 gcgttgcgcc gggcgggtgt gggcctgtgg gacaccgtcg cctcggcccg ccggctcggc
      301 agcaccgacg cggcgatccg cgatcacgcc cccaatgatc tcaaggcgct cgccgcgacc
      361 cttcccaccc tgcgcgcggt cggcttcaac ggcgccacgg cggcgaaatt gggcaccaaa
      421 gcgctcttgg gcacgccttt agcgctgatc gccctcccgt cgagcagtcc gctccacacc
      481 gtcgggctgg aggcaaagct cccagcctgg atggtattgc gcgcctgggt ggacgcgtga
      541 tcggcggctc ccgcgccttc gcggttgaag ggccgcgcga cgcaggctaa gcgcgctgca
      601 tgggcatcaa cctcaatccc ttcacctggt ggaccggcgc gacctggggc acgatggccg
      661 gccttctcgg caagacgcgg gtcggcgagg acgcgctggg caacgtctat tatcagggcg
      721 ggcgcgatac cgccgggcag ccgcgccggt gggtgatcta tgacggcgcc aacgacgcca
      781 gccggatcgc gcccgaatgg ttcagctggc tgcaccatca ggtcgacgac gtgcccgaac
      841 gcgcgctgcc cgcgccgcgc cgctgggaga agccggcggt gcccaacatg accggcaccg
      901 cgctcgccta tcgcccgccc ggcgcgctgg aaaagggcgg ccggcgcgcc gccgcgaccg
      961 gcgactacga agcctggaca cccgagggat gacgcgacgg cagctcgccg tcggggcggc
     1021 gatcgcggtc ctgatcgggt tgagcggttg ggtcggctat cgctggtggg acagccgcca
     1081 ggccgccgag caggcggcgc agcgcccgcc ttcggcgctg gacgagcagg aaagcgcctc
     1141 caacgtcacg atggtccaga tgcccgaggg cgcggacccg tcctacggcg cgacgccgat
     1201 ggctgagcgc gtcgcggtgc tcgggctgct caacaagcgc aacggccaga cccgcgacgt
     1261 gacgctcaag cccggccagg ccacgcgcgt cggcgacgtc atcatccgcc tgcgcgcctg
     1321 cgagcgcacg gcgccgtggg aagatcagga tttgaccggc gccttcgtgc agctcgacgt
     1381 gaagcagttc gacaacagct ggcggcgcgt cttctcgggc tggctctaca aggagcgccc
     1441 cgcgctcaac gtcgtgcagc acccgatcta cgacgtctgg cccaagagct gcgcgatgac
     1501 cttccccgaa ggcggcccca attccacgtc cgcgggcagc gcggcgtcca cgccggcgcc
     1561 gagccggtcg agcgcgccga
//
LOCUS       DASVLL010000450         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_460250,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000450 DASVLL010000000
VERSION     DASVLL010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460250"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..841
                     /locus_tag="VF402_07465"
     CDS             116..841
                     /locus_tag="VF402_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX7800172.1"
                     /translation="MSNKPPPPDDEPRRRMIKPPTGGNLSGRAVGHTAVKTAKRRTAS
                     SAKWLERQLNDPFVQKAKAEGWRSRAAFKLIELDERFHLLHRGAKVVDLGCAPGGWVQ
                     VAIQRGAEKVVGVDLLPVDPVPGAELIEADFTDPAIGPRLMHMLGDKPNLVLSDLAHN
                     TVGHRQTDHLKIMSLLELASDFALDNLKTGGAFAAKAFQGGETEDLLLKLKRRFETVR
                     HFKPKSSRADSSEIYIVAMGFRG"
     gene            complement(838..1296)
                     /locus_tag="VF402_07470"
     CDS             complement(838..1296)
                     /locus_tag="VF402_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800173.1"
                     /translation="MSDAPLKPPRPAVLIVDDDAFVVDSMKIMLGTLGYRSLSAPGAV
                     KGLAFAVKHRPQAILLDLSMPEVDGFAFLLHQRKLPEIQDIPVIVLTASHDKTDVQRA
                     VQLGAADYLVKPLEVDQLARRLERFAPSPLYTPANASVVDWGVKFNKSLL"
     gene            complement(1325..>1768)
                     /locus_tag="VF402_07475"
     CDS             complement(1325..>1768)
                     /locus_tag="VF402_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800174.1"
                     /translation="RAVFGRRGPAQAATAAARRRPKLLVVDDDPAVRDLFLTLLSDEA
                     DVLLAVNSDDALRVMRLHRPDLVLLDDIMPGAVTGLKFLENLQASGEFAATPIVMITA
                     SDAPEHIARGARAGAAGYITKPFDARLVAAMVRDRLGLPFLGQPL"
BASE COUNT          312 a          557 c          616 g          283 t
ORIGIN      
        1 accggtgcgg gccatgcatg actctccttc ggacgacata acagcatacc cgatcgccgc
       61 gttacgcgaa attctgtttt gcgcaacgac ggcaaatccc ggtatagagt ggcgtatgag
      121 taacaagccg cctcccccgg acgacgaacc gcgccgccgc atgatcaagc cgccgacggg
      181 cggaaatctg tccggccgcg cggtcggcca caccgccgtc aagaccgcca agcgccgcac
      241 ggcctcgtcg gccaagtggc tggagcggca actgaacgat ccgttcgtgc agaaggccaa
      301 ggccgagggc tggcgctcgc gcgcggcgtt caagctgatc gaactcgatg agcgctttca
      361 tctgctgcat cgcggcgcga aggtggtcga tctgggctgc gcgcccggcg gctgggtgca
      421 ggtcgccatt cagcgcggcg cggaaaaggt ggtcggcgtc gatcttctgc cggtcgatcc
      481 ggtgccgggc gccgagctga tcgaggccga cttcaccgat ccagccatcg ggccgcgcct
      541 gatgcacatg ctgggcgaca agcccaatct ggtgctgtcc gatctggcgc acaacacggt
      601 cggccaccgc cagaccgatc acctgaagat catgagcctg ctggaactgg cgtcggattt
      661 cgcgctcgac aacctgaaga ccggcggggc cttcgcggcc aaggcgtttc agggcggcga
      721 gaccgaagac ctgctgctga agctgaaacg gcggttcgag acggtcaggc atttcaagcc
      781 gaagtccagc cgcgccgatt cctcggaaat ctacatcgtg gcgatgggat tcaggggcta
      841 aagaaggctc ttgttgaact tgacgcccca atcgaccacc gaggcgttgg cgggcgtata
      901 tagcggactg ggggcgaagc gctccagccg ccgcgccagt tggtccacct ccagcggttt
      961 gaccagatag tccgccgcgc ccagttgcac ggcgcgctgg acgtcggtct tgtcgtggct
     1021 ggcggtcagc acgatcaccg gtatgtcctg aatctccggc agcttgcgct ggtgcagcag
     1081 aaaggcgaag ccgtcgacct ccggcatgga caggtccagc agaatcgcct gcggtcggtg
     1141 cttgaccgcg aaggccaggc ccttcacggc gccgggcgcg ctgaggctgc ggtagcccag
     1201 cgtccccagc atgatcttca tggagtcgac gacgaaggcg tcgtcgtcga cgatcagcac
     1261 ggcggggcgg ggcggtttga gcggggcgtc ggacatgtgc gctccttatc tataagggca
     1321 ggatctataa gggctggccg agaaagggca ggcccaggcg gtcgcgcacc atagcggcga
     1381 cgaggcgggc gtcgaaaggc ttggtgatgt aaccggcggc gccggccctg gcgccgcgcg
     1441 cgatgtgttc cggcgcgtcg ctggcggtga tcatgacgat gggcgtcgcg gcgaattcgc
     1501 cggaggcttg caggttttca aggaatttca ggccggtcac ggcgccgggc atgatgtcgt
     1561 ccagcaggac cagatcgggc cgatgcaggc gcatgacgcg cagcgcgtcg tcgctgttga
     1621 ccgccagcag cacgtccgcc tcgtccgaca gcaacgtcag aaataagtcg cgcacggcgg
     1681 ggtcgtcatc gaccaccagc agcttcggcc tgcggcgcgc ggcggccgtg gcggcttgcg
     1741 ccgggccgcg ccggccgaaa acggcgcg
//
LOCUS       DASVLL010000451         5552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_29338, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000451 DASVLL010000000
VERSION     DASVLL010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29338"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1027
                     /locus_tag="VF402_07480"
     CDS             218..1027
                     /locus_tag="VF402_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7800175.1"
                     /translation="MCPFDPASRPMEPLLSIRDASVVRGTRALIDRLSLDLLPGQHTA
                     ILGPNGSGKSTLVKLITRQLYPLAGGEVRVFGQDRWNVTELRSLMGIVSSAMQLDFDA
                     HPPLEAFDCVVSGFFAARGLWKHHKVTDEMRTHADEAMARIGATHLKGRDMSSLSTGE
                     ARRVLIARALVHRPRALLLDEPCAGLDPASRRGFLEALRGVAATTTLLLVTHHIEEIL
                     PEIGHIVMLKDGRVFREGPKAALLTTDALTALFGMPVEVAPRGDWYHATID"
     gene            complement(1078..2526)
                     /gene="otsA"
                     /locus_tag="VF402_07485"
     CDS             complement(1078..2526)
                     /gene="otsA"
                     /locus_tag="VF402_07485"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HEX7800176.1"
                     /translation="MSRLIVVSNRVNPPADTGVGTAGGLAMALSAALREDKGLWFGWS
                     GETVEHYSGQMSMQVVGGVTVALLDLEEQDRDEYYNGYANRTLWPLFHYRTDLVNYER
                     SFDEGYERVNTRFAETLLPLIGPDDVIWVQDYHLIPLGRELRKRGVKNRIGFFLHIPW
                     PARRVLNTLPRHAELVESMFAYDLIGFQTGDDAEAFNDYIVHDLGAETFEDGAARWRD
                     RVTRVRAFPIGIDAADFAATLKSDKAREYRDLMIHSQGGRKMILGVDRLDYSKGLEER
                     FAAYETLLEKNPDLHSHVFFLQIATRSRDDVDAYQDLRARLDTVSGQINGAYATVEWV
                     PLRYVNRPYRRDELAGIYASAHVGLVTPLRDGMNLVAKEYVASQPPEDPGVLVLSEFA
                     GAAEQMKEALIVNPMSREDVAEAIRKGLSMPLKERKARWELLMDKVQKGDVSRWRDDF
                     VSALKAPKTAPGARPRVVPMSAAAVKIQSTVA"
     gene            complement(2523..3287)
                     /gene="otsB"
                     /locus_tag="VF402_07490"
     CDS             complement(2523..3287)
                     /gene="otsB"
                     /locus_tag="VF402_07490"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX7800177.1"
                     /translation="MSRAAPPRTLRLDDAALFLDLDGTLAPFSATPDGVGPDPLRNGL
                     LRDLARRLGGRLAVISGRSIADLDRILDNSVLCVAGSHGLQRRDARGHVTAAPVHPGV
                     RVAADAFDDFAQARPGVLVERKPLSVALHYRNAPQAEGDVQALALELARATGLKLQLG
                     ACVAELVTLGADKGQALAAFMREAPFKNAAPVFIGDDLTDEDGFRAAAELGGYGVLVG
                     PPRPTRAQCRLDSSAAVIAWLQDLLPAERLSLECVS"
     gene            3674..5488
                     /locus_tag="VF402_07495"
     CDS             3674..5488
                     /locus_tag="VF402_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7800178.1"
                     /translation="MPPNLDLAPIGNCNATALIDRNGRFVWTCYPRVDGEPVFSALLE
                     PSCGGSDKAGFWAVEMTDLKSVRQAYIRNTAVLRTEMEDAHGAVLEVIDFAPRLYNLN
                     RTYRPWAFFRLVRPVKGTPRLRIKMTPTGNYGRDRARETYGSSHVRYTCGKHTLRLTT
                     NAPLSYLGDGDQDDDSAPPFRLEQPLAFHLGPDEPYPADVLADAMHMMHTTISYWQAW
                     TRTLTLPLEWQEAVIRAAITLKLCAYEDTGAIVAAMTTSIPEAADTGRNWDYRYCWIR
                     DAYYVVRALNRLGAADMLESYLVYLRNLVDMAAGGHVQPLYSVALESTLVERVAPDLK
                     GYRGMGPVRIGNQAYEHHQHDVYGQIILPLTQSFFDRRLLRLGSEAEFRAMEKVGERA
                     FAVYDTPDAGLWEFRTRASIHTYSALLCWAACDRLANCAASLGLVDSEALWTERAAII
                     RKAIETRAWNADKGYFAASFEGDELDASLLQLFELGFLPANDPRCTATFKAVETALRK
                     GDNLHRYIEPDDFGEPETAFNFCTFWYIEALYMQGEIDEARRLFEAMLARRTSSGLLS
                     EDVAVETGELWGNFPQTYSLVGLISCATLLSKPWATIR"
BASE COUNT          898 a         1919 c         1793 g          942 t
ORIGIN      
        1 agccgccgat cctcgggtga ggggccgccc gccgactgcg cgcaggcccc tcacccgatc
       61 gccttcgctg acgctcggct ctcgtcctct ccccattgtc atggggagag gggaaggttt
      121 gcggttatag cggctggcgc atgggtttag aatgcgaatt ttcgcctttc ctccttttgc
      181 gcaaacgcct acatgtcccg gcgaaggagt cccccgcatg tgtccgttcg atcccgccag
      241 ccgcccgatg gagcctttgc tgtcgatccg cgacgcctcg gtcgtgcgcg ggacgcgcgc
      301 gctgatcgac cggctgagcc tcgacctctt gccgggccag cacacggcga tcctcggccc
      361 caacgggtcg ggcaaatcca cgctggtcaa gctgatcacg cgccagcttt atccgctggc
      421 gggcggcgag gtgcgcgtct tcggtcagga ccgctggaac gtgaccgaac tgcgcagcct
      481 gatgggcatc gtctcgtcgg ccatgcagct cgatttcgac gcccatccgc cgctggaagc
      541 cttcgattgc gtcgtgtccg gcttcttcgc cgcgcgcggc ctgtggaagc atcacaaggt
      601 cacggacgag atgcggacgc acgccgacga ggcgatggcg cgcatcggcg ccacgcatct
      661 gaaagggcgc gacatgtcga gcctgtcgac cggcgaggcg cggcgcgtgc tgatcgcgcg
      721 cgccctcgtc caccgtccgc gcgccctgtt gctggacgag ccgtgcgcgg gcctcgatcc
      781 ggcttcgcgg cgcggctttc tcgaagcgtt gcgcggcgtc gcggccacga ccaccctgct
      841 gctggtcacg caccatatcg aggaaatcct gccggagatc ggccatatcg tcatgctgaa
      901 ggacggccgg gtgttccgcg aaggccccaa ggccgccctg ctcaccaccg acgcgctgac
      961 cgccctgttc ggcatgccgg tcgaggtcgc gccgcgcggc gactggtatc acgccacgat
     1021 cgactgaatc cggcgcagag ccgcgctcat agggacgact ctgggcggcg acgggcatca
     1081 cgccacggtc gactgaatct taaccgccgc cgcgctcatg ggcacgaccc ggggccgcgc
     1141 gcccggcgcc gtcttcgggg ctttcagcgc gctgacgaaa tcgtcgcgcc agcgcgacac
     1201 gtcgcccttt tgcaccttgt ccatcagcag ctcccagcgc gccttgcgct ctttcagcgg
     1261 catggaaagg cccttgcgga tcgcctcggc cacgtcctcg cggctcatcg ggttgacgat
     1321 cagcgcctcc ttcatctgct cggcggcgcc cgcgaattcc gacagcacca gcacgccggg
     1381 gtcttcgggc ggctgcgagg cgacatattc cttggccacc aggttcatgc cgtcgcgcag
     1441 cggcgtcacc agcccgacat gcgccgaggc gtagatgccc gccagttcgt cgcggcgata
     1501 gggccggttg acgtagcgca gcggcaccca ctccacggtc gcataggcgc cgttgatctg
     1561 gcccgacacg gtgtcgagcc gcgcgcgcag atcctgatag gcgtcgacgt cgtcgcgcga
     1621 gcgcgtggcg atttgcagga aaaacacatg gctgtgcagg tcgggattct tctccagcag
     1681 agtctcgtag gcggcgaagc gctcctccag ccccttggag tagtcgagcc ggtcgacgcc
     1741 gaggatcatc ttgcgcccgc cctgactatg gatcatcagg tcgcggtatt cgcgcgcctt
     1801 gtcgcttttc agcgtggcgg cgaagtcggc ggcgtcgatg ccgatcggga aggccctgac
     1861 gcgcgtgacg cggtcgcgcc agcgcgccgc gccgtcctcg aaggtctcgg cgccgagatc
     1921 gtggacaatg tagtcgttga acgcctcggc gtcgtccccg gtctgaaagc cgatcaggtc
     1981 gtaggcgaac atcgattcga ccagctcggc gtgacgcggc agcgtgttca gcacccgccg
     2041 cgcgggccac ggaatatgca ggaagaagcc gatgcggttc ttcacgccgc gcttgcgcag
     2101 ctcgcgcccc agcgggatca ggtgatagtc ctgcacccag atcacgtcgt ccggcccgat
     2161 cagcggcaac agcgtctcgg cgaagcgcgt attgacgcgc tcataccctt cgtcgaagct
     2221 gcgctcgtaa ttcacgaggt cggtgcggta gtggaacaat ggccatagcg tgcggttggc
     2281 gtagccgttg taatattcgt cgcggtcctg ctcctcgagg tcgagcaggg cgacggtgac
     2341 gccgccgacc acctgcatcg acatctggcc ggaatagtgt tcgaccgtct cgccgctcca
     2401 gccgaaccac agccccttgt cttcgcgcag ggccgcgctc agggccatgg ccagacctcc
     2461 ggcggtgccg acgccggtgt cggcgggcgg gttgacgcgg ttggaaacga cgatcagacg
     2521 gctcacgata cacactccag ggacaggcgt tcggcgggca agaggtcttg cagccaggcg
     2581 atgacggcgg cggacgagtc caggcggcat tgcgcgcgcg tcgggcgcgg cggcccgacc
     2641 agcacgccgt agccgcccag ttcggcggcg gcgcggaagc cgtcctcgtc cgtcaggtcg
     2701 tcgccgatga aaaccggcgc ggcgttcttg aacggcgcct cgcgcatgaa ggcggccaga
     2761 gcctgcccct tgtcggcgcc cagcgtaacc agttcggcca cgcaggcgcc cagttgcagc
     2821 ttcagccctg tggcgcgcgc cagttccagc gccagcgcct gcacgtcgcc ctcggcctgc
     2881 ggcgcgttgc ggtaatgcag cgccacgctc agcggcttgc gctcgaccaa tacgccgggg
     2941 cgcgcctgcg cgaaatcgtc gaaggcgtcg gcggcgaccc gcacgcccgg atggaccggc
     3001 gccgccgtga catggccgcg cgcgtcgcgc cgttgcaggc cgtggctgcc cgccacgcac
     3061 aggacgctgt tgtcgagaat gcggtcgagg tcggcgatcg agcgcccgct gatcaccgcc
     3121 agccgcccgc ccagccgccg cgccagatcg cgcagcaacc cgttgcgcag cggatcgggg
     3181 ccgaccccgt ccggcgtcgc gctgaacggc gccagcgtgc cgtccagatc gaggaacagg
     3241 gcggcgtcat cgaggcgcag tgtgcgcggc ggcgcggcgc ggctcatgcc cgccgccttt
     3301 taccgcatcg cgcgccatcc aagactgtca cgtccaagac tgtcatccgg ccccatctcc
     3361 tcttttcacc gtccggctat ttcagttaaa gcttcccgaa cattgcggcg atcaagtaat
     3421 aaaacttata gaacgcaaag aaatttcgac aaatcgaata gatgttcgga aaataaaaat
     3481 tccataaacg gaattcaaca agagagtcag aggttagcgt caacgattgc cattgtccag
     3541 acctgagcct atatttcgcc acgatctcgc gtttctgtga tgacgtgcgc cgtcattcgt
     3601 gaatggcgtc ggcgcgcctt acgatggtaa gccgatattt ctccgctatg ttttcgcgtc
     3661 caaggagccc tccgtgccgc ccaatctcga tctggccccc atcggcaact gcaacgccac
     3721 cgccctgatc gaccggaacg ggcgcttcgt ctggacctgc tatccgcgcg tcgacggcga
     3781 gccggtgttt tccgccctgc tggagccgtc gtgcggcggt tcggacaagg ccggtttctg
     3841 ggcggtcgag atgaccgacc tgaaaagcgt gcgtcaggcc tatatccgca acaccgccgt
     3901 gctgcgcacc gagatggagg acgcgcacgg cgccgtgctg gaggtgatcg acttcgcgcc
     3961 gaggctttac aatctcaacc gcacctatcg tccgtgggcc tttttccggc tggtgcggcc
     4021 ggtgaagggc acgccgcgcc tgcgcataaa aatgacgccg acggggaatt acggccgcga
     4081 ccgcgcgcgc gaaacctatg gctccagcca cgtgcgctac acctgcggca agcacaccct
     4141 gcgcctgacc accaacgcgc cgctcagcta tctcggcgac ggcgatcagg acgacgacag
     4201 cgcgccgccc ttccggctgg agcagccgct ggcctttcac ctcggcccgg acgagccgta
     4261 tcccgccgac gtgctggccg acgccatgca catgatgcac accacgatca gctactggca
     4321 ggcgtggacg cgcaccctca ccctgccgct ggaatggcag gaggcggtga tccgcgccgc
     4381 catcacgctg aaactgtgcg cctatgagga caccggcgcc atcgtcgccg ccatgaccac
     4441 ctcgatcccc gaagccgccg atacggggcg caactgggac tatcgctact gctggatccg
     4501 cgacgcctat tacgtggtgc gggcgctgaa ccggctgggg gcggcggaca tgctggaatc
     4561 ctatctcgtt tacctgcgca acctcgtcga catggcggcg ggcgggcatg tgcagccgct
     4621 ctattccgtg gcgctggaat cgacgctggt cgagcgcgtc gcgccggacc tgaagggcta
     4681 tcgcggcatg gggccggtgc gcatcggcaa ccaggcctac gagcatcacc agcacgacgt
     4741 gtacggccag atcatcctgc cgctgaccca gtccttcttc gaccggcgcc tgttgcgcct
     4801 cggctcggag gccgagttcc gcgccatgga gaaggtcggc gagcgcgcct tcgccgtcta
     4861 tgacaccccc gacgccgggc tgtgggagtt ccgtacccgc gccagcatcc acacctattc
     4921 ggcgctgctg tgctgggcgg cgtgcgaccg gctggccaac tgcgccgcca gcctgggcct
     4981 tgtcgactcc gaggccttgt ggaccgagcg cgccgccatc atccgcaagg ccatcgagac
     5041 gcgcgcctgg aacgcggaca agggctattt cgccgcctcg ttcgagggcg acgaactgga
     5101 cgccagcctg ctgcaactgt tcgagctggg cttcctgccg gccaacgatc cgcgctgcac
     5161 agcgaccttc aaggcggtcg agacggcgct gcgcaagggc gacaatctcc accgctatat
     5221 cgagccggac gatttcggcg agccggagac ggcgttcaac ttctgcacct tctggtacat
     5281 cgaggcgctg tacatgcagg gcgagatcga cgaggcgcgc cgcctgttcg aggccatgct
     5341 ggcgcgccgc acctcatcgg gcctgctctc cgaagacgtc gcggtcgaga ccggcgagtt
     5401 gtggggcaac ttcccgcaga cctattcgct ggtcggcctg atcagttgcg cgaccctgct
     5461 gtcgaagcca tgggcgacga tccgctaaaa cacccccctt aaaacaccgt cgtcacgtcc
     5521 ggcgccagcg gatcgggtcc gtacttgttc gg
//
LOCUS       DASVLL010000452         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_29342, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000452 DASVLL010000000
VERSION     DASVLL010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29342"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VF402_07500"
     CDS             <1..1638
                     /locus_tag="VF402_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7800179.1"
                     /translation="HDELTLIGIIRPGEPVGEMSLIAGTQHTSTVMALRDSDLLAMPR
                     EDFLHALDTRPELLLALSRKMMERARREAPNVAPNVFAFFALGEPAIGAFIEAVAVQI
                     RELGYRVAVIDKGFHGATPEAFTQIEAENDYVLHIAESHESHWRLLSARQVDHVFLVA
                     DAAHAPDARATAWEKSLLHRAPDLILTYPANQKKAHVKGRTRAWLDVIKPTRWFHIHT
                     ESLPDAARIARVICGHSVGVVFSGGGARAFSQIGAIQALREAHLPIDFVCGSSMGAII
                     AAGLALGWDDSEIDWRMRDAFVNSSPLDDVTFPFISMVSGRKVDKRLEKHFGDIAIED
                     IHLPFFCLSSNLTSGVIKLHKTGLLRKALRASISLPGVLPPVVDDGQVLVDGAVMRSF
                     PATMMRNTHLGTVIGIDVTRARGLDPKSLVTPKNLSSWFTHGDWRRGPPIVSIMMRSA
                     TIITAADLAVSRAATDLLIIPEPDGVEIRDWKAYDKAVENGYQTTVATLAQLDSPVTT
                     LRKTGRSIHPDIPAFTPDDTYVETATEPPRKRNEGGS"
     gene            complement(1635..>2224)
                     /locus_tag="VF402_07505"
     CDS             complement(1635..>2224)
                     /locus_tag="VF402_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800180.1"
                     /translation="PVFQSASVMPPPVITAINPYARPQTPAPQPEAAEVRAVAPAADV
                     TQAPFVQASPLNWREQLQRPLPQAEPDPAETPPPMTAARGASHFDEDYGDDDGWTLDG
                     GRVAVSNETSEPEHASWWTMIVSTFWWIFISGVGLGCLGVAAGAYYKSKDLAVIRNGA
                     FGDYMTWSIIMAAVGILCVSISVWLIMRRLGGLKD"
BASE COUNT          369 a          805 c          699 g          351 t
ORIGIN      
        1 cacgacgaac tgacgctgat cggcatcatc cgccccggcg agccggtcgg cgagatgtcg
       61 ctgatcgcgg gcacgcagca cacctcaacc gtcatggcgc tgcgcgattc cgacctgctg
      121 gccatgccgc gcgaggattt cctgcacgcg ctcgacaccc ggccggaact gctgctggcc
      181 ctgtcgcgca agatgatgga gcgcgcgcgc cgcgaagcgc ccaatgtcgc gccgaacgtc
      241 ttcgccttct tcgcgctggg cgagcccgcg atcggcgcct tcatcgaagc cgtggcggtc
      301 cagattcgcg aactgggcta ccgcgtcgcc gtgatcgaca agggctttca cggcgccacg
      361 cccgaagcct tcacccagat cgaagccgaa aacgactatg tgctgcacat cgccgagtcg
      421 cacgaaagcc actggcgcct gctgagcgcg cgtcaggtcg atcacgtctt tctggtcgcc
      481 gacgccgccc atgcgcccga cgcgcgcgcc acggcgtggg aaaagtcgct gctgcaccgc
      541 gcgcccgacc tgatcctgac ctatccggcc aaccagaaga aggcgcacgt caagggccgc
      601 acccgcgcct ggctcgacgt gatcaagccg acgcgctggt tccatatcca caccgaatcg
      661 ctgcccgacg cggcgcggat cgcgcgcgtc atctgcggcc attcggtcgg cgtggtgttt
      721 tccggcggcg gcgcgcgcgc cttttcgcag atcggcgcca ttcaggccct gcgcgaagcc
      781 catcttccca tcgacttcgt atgcggctcg tcgatgggcg cgatcatcgc cgccggtctg
      841 gcgctgggct gggacgacag cgagatcgac tggcggatgc gcgacgcctt cgtcaattcg
      901 tcgccgctgg acgatgtgac cttcccgttc atctcgatgg tgtcgggccg caaggtcgac
      961 aagcggctgg aaaagcattt cggcgacatc gccatcgagg acatccacct gcccttcttc
     1021 tgcctgtcgt ccaacctgac ctccggcgtg atcaagctgc acaagaccgg ccttctgcgc
     1081 aaggccctgc gcgcctcgat ctcgctgccc ggcgtgctgc cgcccgtggt cgacgacggt
     1141 caggtgctgg tggacggcgc ggtcatgcgc tccttcccgg ccaccatgat gcgcaacacc
     1201 catctcggca cggtgatcgg catcgacgtg acgcgcgcgc gcggcctcga tccgaagtcg
     1261 ctggtcacgc cgaaaaacct gtcgagctgg ttcacgcacg gcgactggcg gcgcgggccg
     1321 cccatcgtct cgatcatgat gcgttcggcc accatcatca ccgccgccga tctggccgtg
     1381 tcgcgcgccg cgaccgatct gctgatcatc cccgagccgg acggcgtcga aatccgcgac
     1441 tggaaggctt acgacaaggc ggtcgagaac ggctatcaga cgacggtggc cacgctggcg
     1501 caactcgatt cgccggtcac caccctgcgc aagacgggcc gcagcatcca cccggacatt
     1561 ccggccttca cgccggacga cacctatgtc gagaccgcca cggagccgcc gcgcaaacgc
     1621 aacgaaggcg gcagctaatc cttgagcccg cccaggcggc gcatgatcag ccacaccgag
     1681 atcgacacgc acaatatgcc gaccgcggcc atgatgatcg accaggtcat atagtccccg
     1741 aaggcgccgt tgcggatcac cgccagatcc ttcgacttgt aataagcgcc ggcggcgacg
     1801 cccagacagc ccagcccgac gccgctgatg aagatccacc agaaggtcga gacgatcatc
     1861 gtccaccagc tggcgtgttc aggctcgctc gtctcgttcg acaccgccac gcggccgccg
     1921 tccagcgtcc agccgtcgtc gtcgccgtaa tcctcatcga aatggctggc gccgcgcgcc
     1981 gccgtcatcg gcggcggcgt ttccgccggg tccggctcgg cctgcggcag cggccgctgc
     2041 aactgctcgc gccagttcag cggcgacgcc tgtacgaacg gcgcctgcgt gacatcggcg
     2101 gccggagcga cggcgcggac ctcggccgcc tccggttgcg gcgcgggcgt ttgcggacgc
     2161 gcatagggat tgatcgccgt aatgaccggc ggcggcatga ccgacgcgct ctggaacacg
     2221 ggcg
//
LOCUS       DASVLL010000453         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_138247,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000453 DASVLL010000000
VERSION     DASVLL010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138247"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF402_07510"
     CDS             complement(<1..285)
                     /locus_tag="VF402_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX7800181.1"
                     /translation="MSFTGISANRQELLQIAEAVAREKAIDKSVVIASIEEAIQKAAR
                     SRYGADHDIRVHIDPRTGEMTITRVTTVVEDELLENEYAQKTLADALRDDK"
     gene            complement(289..894)
                     /gene="rimP"
                     /locus_tag="VF402_07515"
     CDS             complement(289..894)
                     /gene="rimP"
                     /locus_tag="VF402_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7800182.1"
                     /translation="MRAKTAEDRKLLDLFEPLAEAQGLNIVRIRMMGSSRAGGARRLQ
                     IMAERKSDGDINVNECARLSRAISALVDEADPISGEFVLEVSSPGIDRPLTRLEDFAT
                     YEGLEARIELDRLAEGRKRFRGVLAGIEGDHIAIDLEGEDETAMIPFAWVVEAKLVLT
                     DDLMKRGAEARAARGDEDEDEDEDFGDDDYEDDVANDDEED"
     gene            complement(1277..1963)
                     /gene="trmB"
                     /locus_tag="VF402_07520"
     CDS             complement(1277..1963)
                     /gene="trmB"
                     /locus_tag="VF402_07520"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX7800183.1"
                     /translation="MSDEEKSAAPTWGPMRSFGRIKSRTLKPRQASLFDTLLPRIAAD
                     DAHVTAALAKGPVWLEIGFGGGEHLAQQAARNPDVTIIGCEPFLNGVGSVLRHVEDGG
                     LDNVLILADDARPLLARLPDAALARIFILFADPWPKARHNKRRFIQTETVAEFARVLK
                     PGGIARFATDWADYADWALERFLANPDFIWEAQAPEDWTQPPADHVTTRYETKGLGDC
                     KPVFFDFARR"
     gene            complement(2021..>2439)
                     /locus_tag="VF402_07525"
     CDS             complement(2021..>2439)
                     /locus_tag="VF402_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX7800184.1"
                     /translation="RLITEPAAAALAAQRREPKHLDIMDKALNVMASQTLATEAGRKA
                     DLDFHNALLDATKNEALICLSSSIGAAVSWSTRFKQRHSALPRDPVPDHRAVYEAIRR
                     QDPNDARWCMETLVRLALRDTQASMDGATLGTAPQL"
BASE COUNT          402 a          787 c          795 g          455 t
ORIGIN      
        1 cttgtcgtcg cgcagcgcgt cggccagcgt cttctgcgcg tattcgttct ccagcagctc
       61 gtcctcgacg accgtggtga cgcgggtgat ggtcatttcg ccggtgcgcg gatcgatgtg
      121 aacgcgaatg tcgtgatcgg cgccgtagcg cgaacgcgcc gccttctgga tcgcttcctc
      181 gatggaggcg atgaccacgg acttgtcgat cgccttttcg cgggcgaccg cttcggcgat
      241 ttgcagcaac tcctggcggt tggcgctgat tccggtaaag ctcatggctt aatcctcttc
      301 atcgtcgtta gcgacgtcgt cttcataatc gtcgtcgccg aaatcttcgt cttcgtcttc
      361 gtcttcgtcg ccgcgcgccg cgcgcgcctc ggcgccgcgt ttcatcaggt cgtcggtgag
      421 gaccagcttg gcctcgacga cccaggcgaa cgggatcatc gcggtctcgt cctcgccttc
      481 gaggtcgatg gcgatatggt cgccctcgat cccggccagc acgccgcgaa agcgcttgcg
      541 cccctcggcc agccggtcca gctcgatgcg cgcctcaagc ccttcgtagg tcgcgaagtc
      601 ctcaagccgg gtcaggggcc ggtcgatgcc gggcgaggac acttccagca cgaattcgcc
      661 ggagatcgga tcggcctcgt cgaccagggc cgagatcgcc cgcgacaggc gcgcgcattc
      721 gttgacgttg atgtcgccgt ccgacttgcg ctcggccatg atctgcaggc ggcgcgcgcc
      781 gcccgcgcgg ctcgacccca tcatgcggat gcgcacgatg ttcagcccct gcgcttcggc
      841 gagaggctcg aacaggtcga gaagcttgcg atcttcggcg gtcttggcgc gcataaaacc
      901 caggttcaga taataaaaaa agcggcgagc cgctcgggcc gccgccgaag ccttgcggct
      961 gtcggacaat gttgccacgc tttataggcg catcatggcc gtttgccaag cgttgatttg
     1021 gctaaaggtt ttcgccacga aaaacacgaa aaggcacgaa atccgcgcgt agcgtactat
     1081 ctatgtttat tggaaccacg gaaaccacgg aaaacacgga cgatcttgtg acgccgcgta
     1141 gcggcgtttc cgtggtttcc gtgttttccg tggtttaaaa aacgaccgtc aggcgaccag
     1201 caggcggtaa gacgctacgc gcggatttcg tgccttttcg cgcctttagt ggcgaaaaaa
     1261 atagtggcga aaaaaatcaa cgccgcgcga aatcgaagaa caccggctta caatcgccca
     1321 gacctttcgt ttcatagcgc gtggtgacat ggtcggcggg cggttgcgtc cagtcttcgg
     1381 gcgcctgcgc ctcccagatg aagtccgggt tggcgaggaa gcgctccagc gcccagtcgg
     1441 cgtaatcggc ccagtcggtg gcgaagcgcg cgatcccgcc gggtttcagc acgcgcgcga
     1501 attcggccac ggtttcggtc tggatgaagc ggcgcttgtt gtgccgcgcc ttcggccacg
     1561 gatcggcgaa caggatgaag atgcgcgcca gcgccgcgtc cggcaggcgc gccagcagcg
     1621 ggcgcgcgtc gtcggcgagg atcagcacat tgtccagccc gccgtcctcg acatggcgca
     1681 ggaccgagcc gacgccgttc aggaacggct cgcagccgat aatcgtcaca tcggggttgc
     1741 gcgcggcctg ctgggccaga tgctcgccgc cgccgaagcc gatctccagc cagaccggcc
     1801 ccttggccag cgctgccgtc acatgcgcgt cgtccgcggc gatgcgcggc agaagcgtgt
     1861 cgaacagcga cgcctgacgc ggcttcaagg tgcgcgactt gatgcggccg aacgagcgca
     1921 tcggccccca tgtgggcgcg gcggattttt cttcatctga catggcggcg tctgtagcgg
     1981 cgaacgcggc aaaagacaat ccgctctaaa tccacccgtc ctaaagctgc ggcgcggtcc
     2041 ccagcgtggc gccgtccatg ctggcctgcg tgtcgcgcag cgccagccgc accagcgtct
     2101 ccatgcacca gcgcgcgtcg ttcgggtctt gccgccggat cgcctcatag acggcgcggt
     2161 gatcgggcac gggatcgcgc ggcagggcgg aatggcgctg cttgaagcgc gtcgaccacg
     2221 acaccgccgc gccgatcgac gaactcaggc agatcagcgc ctcgtttttg gtggcgtcga
     2281 gcagggcgtt gtggaagtcg aggtcggcct tgcgcccggc ctcggtggcc agggtctgcg
     2341 acgccatgac gttcagcgcc ttgtccatga tgtcgagatg cttcggctcg cgccgctgcg
     2401 ccgccagcgc ggcggcggcc ggttcggtga tcaggcgca
//
LOCUS       DASVLL010000454         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_299578,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000454 DASVLL010000000
VERSION     DASVLL010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299578"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VF402_07530"
     CDS             <1..246
                     /locus_tag="VF402_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0032981 - mitochondrial respiratory chain
                     complex I assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase subunit NDUFA12
                     family protein"
                     /protein_id="HEX7800185.1"
                     /translation="KRRWVIYKGYADASKIPPDWHGWMHYIYDTPPSEQPLRRKAWEK
                     PHEPNLSGTPFARFPSGSLNAEGQRQASAGDYEAWKP"
     gene            258..1004
                     /locus_tag="VF402_07535"
     CDS             258..1004
                     /locus_tag="VF402_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEX7800186.1"
                     /translation="MSGPRFKLSVLLALSGLTVAFAVIPAGAQVAPPSRDGGGNDDAP
                     AYVDNADRQPYYVDGVEYRPYVPEKHKAKSTDAPPPEEQPETAPAGSQLHPYAPPASA
                     PAGPQDSIVASAAPVSASSSSSSSRSASLTPQKRPRYAIAVIQALDKVTAETIRFEAP
                     IGKPVRYKGLIYTARACETTTPDEAAPDVIAYLEVRTNPAPATSNAPAVRSREIFHGW
                     SFASAPSLNPIEHPNYDAWVIGCRQPLPGV"
     gene            complement(983..1642)
                     /gene="aat"
                     /locus_tag="VF402_07540"
     CDS             complement(983..1642)
                     /gene="aat"
                     /locus_tag="VF402_07540"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX7800187.1"
                     /translation="MADFTLDDLIECYRTGVFPMSDARDDEYLFLVDPPLRGVLPLDG
                     FHLPSRLARTVRKDDYAIRVDTAFAEIIELCAEEARNRTSTWISHAIQDLYQALFARG
                     LAHSVEVWRGDDLVGGLYGVAIGGAFFGESMVSRARDASKIALVHLAARLCAGGFTLL
                     DCQFQTEHLRQFGVIEIPKADYLKKLADAVDRKADFYLLPRRTTGRQALDALAQTPGR
                     G"
     gene            complement(1736..2173)
                     /locus_tag="VF402_07545"
     CDS             complement(1736..2173)
                     /locus_tag="VF402_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800188.1"
                     /translation="MIRPAALLPLLAACALYAGAAQAGQDDYAYKTSTLRNVVISDYG
                     KRPAPSGLLRTWLRIGVVENHLASLDVYLNYQGEAQKLPPLGATCDIVYHMGWIEGYF
                     KPLQSAADRDRVIDDFTCRADSKRVVKSAARYPRRLSKARASA"
     gene            complement(2275..>2627)
                     /locus_tag="VF402_07550"
     CDS             complement(2275..>2627)
                     /locus_tag="VF402_07550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517334.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7800189.1"
                     /translation="AIECRLNAENPKTFAASPGLITDFHAPGGLGVRLDSAIYAGYSV
                     PPYYDSLIGKLIVHGRDRTECIARLKRCLGEMVIGGIDTTIPLFLDLLQEKDVQTGDY
                     NIHWLENWIKAQQA"
BASE COUNT          446 a          857 c          895 g          429 t
ORIGIN      
        1 aagcgccgct gggtcatcta taagggctat gccgacgcct cgaagattcc gcccgactgg
       61 cacggctgga tgcactatat ctacgacacg ccgcccagcg agcagccttt gcgccgcaag
      121 gcgtgggaaa agccgcacga gccgaacctg agcggcacgc cgttcgcgcg cttcccgtcg
      181 ggttcgctga acgccgaagg ccagcgtcag gcgtcggcgg gcgattacga ggcctggaag
      241 ccgtaaagag ggtcgttatg agtggaccgc gtttcaagct ttccgtcctg ctggccctgt
      301 ccgggctgac ggtggcgttc gcggtcattc cagcaggcgc gcaggtggcg ccgccgtcgc
      361 gcgacggcgg cgggaacgac gacgcgccgg cttacgtcga taacgccgac cgccagccct
      421 actatgtcga tggggtcgaa taccgcccct acgtgccgga aaagcacaag gccaaatcga
      481 cggacgcgcc gccgccggag gaacagccgg aaacggcccc ggccggttcg caactgcatc
      541 cctatgcgcc gcccgcgagc gcgcccgccg ggccgcagga ttcgatcgtc gcttcggcgg
      601 cgccggtcag cgcgtcgtca tcgtcgtcct ccagccgttc ggccagcctg acgccgcaga
      661 agcgtccgcg ttacgcgatc gcggtcattc aggcgctgga caaggtcacg gcggagacga
      721 tccgcttcga ggcgccgatc ggcaagccgg tgcgctacaa ggggctgatc tacaccgcgc
      781 gcgcctgcga gacgaccacg ccggacgaag ccgcgccgga cgtcatcgcc tatctggagg
      841 tgcgcaccaa tccggcgccc gccaccagca acgcgcccgc cgtgcgttcg cgcgagatat
      901 tccacggctg gagcttcgcc tcggcgccca gcctgaaccc gatcgagcat ccgaactatg
      961 acgcctgggt gatcggctgc cgtcagcccc tgccgggcgt ctgagccagc gcgtccagcg
     1021 cctgacggcc cgtggtccgg cgcggcagca ggtagaagtc cgccttgcgg tccaccgcgt
     1081 cggccagttt tttcagatag tccgccttgg ggatttcgat cacgccgaac tgacgcagat
     1141 gttcggtctg gaactggcag tcgagcaggg tgaagccgcc cgcgcacagg cgggccgcca
     1201 gatggacgag ggcgatcttc gatgcgtcgc gcgcccgcga caccatgctt tcaccgaaaa
     1261 acgcgccgcc gatggccacg ccgtagaggc cgccgacgag atcgtcgccg cgccacacct
     1321 cgacgctgtg cgccagtccg cgcgcgaaca gggcctgata cagatcctga atggcgtggc
     1381 tgatccaggt cgaggtgcgg ttacgggcct cctcggcgca cagctcgata atctccgcga
     1441 acgccgtgtc gacgcggatg gcgtaatcgt ccttgcgcac cgtgcgcgcc agccgcgacg
     1501 gcaggtgaaa accgtccagc ggcagcacgc ccctgagcgg cggatcgacc agaaacaggt
     1561 attcgtcgtc gcgcgcgtcc gacatgggaa acacgccggt gcggtagcat tcaatcagat
     1621 cgtcgagcgt gaagtcggcc aagccgcggt cctgcgtttt gcgagtcgcg cgcgattata
     1681 gacggcgcat cgcggcgatc aatgcgtcaa gtccttgcgt ttcatggctg tccgctcagg
     1741 cggaggcgcg ggctttcgac aggcggcggg gatatcgcgc ggcgcttttg acgacgcgct
     1801 tcgaatccgc cctgcaggtg aagtcgtcga tgacccggtc gcggtccgcc gcgctttgca
     1861 gcggtttgaa atacccctcg atccagccca tgtgatagac gatgtcgcag gtcgcgccca
     1921 gcggcggcag tttctgcgcc tcgccctgat agttgagata gacgtcgagg ctggcgaggt
     1981 ggttttcgac cacgccgatg cgcagccatg tgcgcagcag gccggacggg gcgggccgct
     2041 tgccgtaatc gctgatcacc acgttgcgca gggtcgaggt cttgtacgca tagtcgtcct
     2101 gaccggcctg cgccgcgccg gcgtacagcg cgcaggcggc gagaagcggc aagagggcgg
     2161 cggggcgtat catggcgaca cgctatacct cctcggcgat tcaggcaaaa gaaaagcctt
     2221 gcgtccggca aggcttttcc atcgcggaac gggtcggccc ggaaccggcc ggatctacgc
     2281 ctgctgggcc ttgatccagt tttccagcca gtgaatattg tagtcgccgg tctgtacgtc
     2341 cttttcctgc aacaggtcga ggaacagggg gatggtggtg tcgatgccgc cgatgaccat
     2401 ctcgcccagg cagcgcttca gccgggcgat gcattcggtg cggtcgcggc cgtggacgat
     2461 cagcttgccg atcaggctgt cgtaataggg cggcacggaa tagccggcgt agatcgccga
     2521 atcgaggcgc acgccgaggc cgccgggggc atggaagtcg gtgatcaggc cgggcgaggc
     2581 ggcgaaggtc ttcgggttct cggcgttgag ccggcactcg atggcgt
//
LOCUS       DASVLL010000455         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_190410,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000455 DASVLL010000000
VERSION     DASVLL010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190410"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="VF402_07555"
     CDS             <1..97
                     /locus_tag="VF402_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800190.1"
                     /translation="EGVENFHFYTLNRADLVYAICRILGLKEAVQ"
     gene            94..1158
                     /locus_tag="VF402_07560"
     CDS             94..1158
                     /locus_tag="VF402_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX7800191.1"
                     /translation="MNRAERIEALKAAARERILILDGSWGVMIQRRGLDEADFRQQRF
                     KDYPGQLKGNNDILCLTRPDIIADLHDQYYAAGADISETNTFSATTIAQDDYRLTLAD
                     VIDINREGAKIARAAADRWTQKEPDKPRFVAGSIGPLNKMLSMSSDVNDPGARLVTFD
                     QVYQAYREQIQALNEGGVDLYLIETITDTLNCKAAIKAILDLEDEGLDRLPIWISGTI
                     TDRSGRTLSGQTAEAFWNSVRHAKPFAVGFNCALGADLMRPHIAELSRVADCLVAAYP
                     NAGLPNAMGQYDEQPHETAHELHEWAKDGIVNILGGCCGTTPDHIKHVADEVRGVAPR
                     QIPERPRALRLAGLEPFELT"
     gene            1158..>1770
                     /locus_tag="VF402_07565"
     CDS             1158..>1770
                     /locus_tag="VF402_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX7800192.1"
                     /translation="MTTTILFGGTSRERLVAVATAQAVAEALPEADLWFWDANDTVWS
                     ASHDALQGHARPFEVPFRADGARIGGLAAAMDRAAAEGRVLVLALHGGSAENGEFQVL
                     AEARGVPFTGSGSAASHLAFDKASAKRFAALAGVASPSTVTLDEADEALAHYGRLIAK
                     PAQDGSSYGLIFVNSAQDLAAVRRESATAPYVIEPFVTGAEATC"
BASE COUNT          306 a          584 c          601 g          279 t
ORIGIN      
        1 cgaaggcgtc gaaaatttcc atttctatac gctcaaccgc gccgatctgg tgtatgcgat
       61 ctgccggatt ttagggttga aggaggccgt ccaatgaacc gggccgaacg catcgaagct
      121 ttgaaagcgg ccgccaggga acgcatcctg atcctcgacg gctcgtgggg cgtgatgatc
      181 cagcgccgcg ggctggacga ggccgatttc cgccagcagc gcttcaaaga ctatccgggc
      241 cagctcaagg gcaataacga catattgtgc ctgacgcgcc cggacatcat cgccgacctg
      301 cacgatcagt attacgcggc gggcgcggac atttccgaaa ccaacacctt ctccgccacc
      361 accatcgcgc aggacgacta tcgcctgacc ctggccgacg tgatcgacat caaccgcgag
      421 ggcgcgaaga tcgcccgcgc cgccgccgac cgctggacgc aaaaggagcc ggataagccg
      481 cgcttcgtcg ccggttcgat cgggccgctg aacaagatgc tttccatgtc gtcggacgtc
      541 aacgatccgg gcgcgcggtt ggtcaccttc gatcaggtct atcaggccta tcgcgagcag
      601 attcaggcgc tcaacgaggg cggggtcgat ctctacctga tcgagacgat caccgacacg
      661 ttgaactgca aggcggcgat caaggcgatc ctcgaccttg aggacgaggg actggatcgc
      721 ctgccgatct ggatttccgg caccatcacc gaccggtcgg gacggacgct gagcggccag
      781 accgccgaag ccttctggaa cagcgtgcgc cacgccaaac cgttcgcggt cgggttcaac
      841 tgcgcgctgg gcgccgacct gatgcgcccg cacatcgcgg agctgtcgcg cgtcgccgac
      901 tgtctggtcg cagcctaccc gaacgcgggc ctgcccaacg cgatgggcca gtatgacgag
      961 cagccgcacg aaaccgcgca tgaactgcat gagtgggcca aggacggcat cgtcaacatc
     1021 ctcggcggct gttgcggcac gacgccggat cacataaagc acgtcgcgga cgaggtgcgc
     1081 ggcgtggcgc cccggcaaat ccccgaacgc ccgcgcgccc tgcgtctggc ggggcttgag
     1141 cctttcgagt tgacctgatg acgacgacga tcctgtttgg cggaaccagc cgcgaacgtc
     1201 tggtggcggt ggcgacggcg caggccgtgg ccgaggcgct gcccgaggcg gacctgtggt
     1261 tctgggacgc gaatgacacg gtgtggtcgg cgtcgcacga cgccttgcaa ggccacgcca
     1321 ggccgttcga ggttccgttc cgggccgacg gcgcgcgcat cggcggtctg gcggcggcga
     1381 tggaccgggc ggcggcggaa ggccgcgttc tggtgctggc cctgcatggc ggctcggcgg
     1441 aaaacggcga gtttcaggtg ctggccgagg cgcgcggcgt gcccttcacc ggctccgggt
     1501 cggcggcctc gcacctcgcc ttcgacaagg cgtcggccaa gcgcttcgcc gctctggcgg
     1561 gcgtggcgtc gccgtcgacg gtgacgctgg acgaggcgga cgaggcgctg gcccactatg
     1621 gccgcctgat cgccaagccc gcgcaggacg ggtccagcta tggcctgatc ttcgtcaatt
     1681 ccgcgcagga tctggccgcc gtgcgccgcg aaagcgcgac ggcgccctat gtgatcgagc
     1741 cgttcgtcac cggcgccgag gcgacctgcg
//
LOCUS       DASVLL010000456         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_460570,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000456 DASVLL010000000
VERSION     DASVLL010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460570"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VF402_07570"
     CDS             complement(<1..500)
                     /locus_tag="VF402_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7800193.1"
                     /translation="MTPDDLSSPTHDEIREYLHRQMLSSVSHDLKTPLATIIGSLEVM
                     TMLYDRLSEEKRRSLITSALTEAFRLDYFITNILDMAKFEADAVRPRMEATRLSQMIQ
                     DAQARLGPLKARGELRITAAGRDELTTDPMLGARAIGLGLHNAYKFGGRKAGRTEDNP
                     LIEASYG"
     gene            complement(758..1114)
                     /locus_tag="VF402_07575"
     CDS             complement(758..1114)
                     /locus_tag="VF402_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800194.1"
                     /translation="MNRSGGFLQTLLIFILLVVVIVAGVWFYNARHRPTAGQQIGRAI
                     DSIPPAVSSAAGDITDSGVYSSAAVALDNAGAAASSALAKTGAAASSAVSETSADVKA
                     AAKKQKQQDRSASASK"
     gene            complement(1111..1338)
                     /locus_tag="VF402_07580"
     CDS             complement(1111..1338)
                     /locus_tag="VF402_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800195.1"
                     /translation="MHPHSQPAQIIPFPARRAQRPARAQNADLNWRVSIDGEFSEDDL
                     PPELFDADEDREALALYAHIADQWADQRNDG"
     gene            complement(1366..1518)
                     /locus_tag="VF402_07585"
     CDS             complement(1366..1518)
                     /locus_tag="VF402_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800196.1"
                     /translation="MPVIPQAPQPDPAPQPDIPQPQEPDVNPAPEPPGTPEPPSEIPP
                     VTRPEV"
BASE COUNT          249 a          473 c          575 g          284 t
ORIGIN      
        1 ccgtaggacg cttcgatcag cggattgtcc tcggtgcggc ccgccttgcg tccgccgaac
       61 ttgtaggcgt tatgcagccc cagtccgatg gcgcgcgcgc ccagcatcgg gtcggtggtc
      121 agctcgtcgc gccccgccgc cgtgatgcgc agctcgccgc gcgccttgag cggccccagc
      181 cgcgcctgcg cgtcctggat catctggctc agccgcgtcg cctccatgcg cgggcgcacc
      241 gcgtcggcct cgaacttggc catgtcgagg atgttggtga tgaaatagtc gagccggaag
      301 gcctcggtca gggccgaggt gatcagcgac cggcgcttct cctcgctgag ccggtcgtac
      361 agcatggtca tgacctccag cgagccgatg atggtcgcca gcggcgtctt gaggtcatgc
      421 gagacggacg acagcatctg gcggtgcaga tactcgcgga tttcgtcatg ggtcggcgag
      481 gacagatcgt cgggagtcat gaacgtattt taccagtacg gccacgccat gcaaaccatt
      541 caaccgtgac ggcgaaggaa aaacggcctg gcaatgcgaa agggagcaag gggaaaaacc
      601 cttgctcccg tctttatgcg acgcggtcgc ctgagggggc gggggacagg cgcgcgtcgg
      661 gctccggtca gtggatgacg tggaacagat agagcagaat gatcaccggc aggggcacgc
      721 cgaccagcca taggatgagg cctttcatga gctttcctta tttggatgcg gacgcgctgc
      781 ggtcctgttg cttttgcttt ttcgccgccg ccttgacgtc ggcggaggtt tccgagacgg
      841 cgctggaggc ggcggcgccg gttttggcca gcgcgctgga cgcggccgcg ccggcgttgt
      901 cgagcgcgac ggcggcgctg gaatagacgc cggagtcggt gatgtcgccc gccgcgctcg
      961 aaacggcggg cggaatggag tcgatggccc ggccgatctg ctggcccgcg gtcgggcggt
     1021 ggcgcgcgtt gtagaaccac accccggcca cgatcaccac caccagcagg atgaagatca
     1081 gcagggtttg caggaagccg ccggaccggt tcatccgtcg ttcctctggt cggcccactg
     1141 atcggcgatg tgagcgtaga gcgccagcgc ctcgcggtcc tcgtcggcgt cgaacagttc
     1201 cggcggcaga tcgtcctcgg aaaattcgcc gtcgatgctc acccgccagt tgaggtctgc
     1261 attctgggcg cgggccgggc gttgcgcgcg ccgcgcggga aagggtatga tctgggccgg
     1321 ttgcgaatgg gggtgcatgg tcgcggtcct cgcaggggaa ggggatcaga cttcaggccg
     1381 ggtcacgggc gggatttccg acggcggttc gggagtcccc ggcggctccg gcgccggatt
     1441 gacgtcgggc tcctgcggct gtgggatgtc gggctgcgga gccggatcgg gctgcggagc
     1501 ctgcggtatg acgggcatga cgcgtatcct gagaagctga actggcttcc agatttaggc
     1561 gaatttctgc aagggctcag t
//
LOCUS       DASVLL010000457         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_83808, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000457 DASVLL010000000
VERSION     DASVLL010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83808"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1201)
                     /locus_tag="VF402_07590"
     CDS             complement(290..1201)
                     /locus_tag="VF402_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7800197.1"
                     /translation="MNLDAARVSLRRMNAPAKPSAEDRRARKAMRREKQRAANPVRAE
                     AMHKARLADLYAGAPRGDAAPACNIGCSGWFYWDWKGLFYPAQMATQDWFGYYADRFD
                     TVELNAPFYSWPTVNAVKGWLRQAGERDFVYTVKVSELITHVRRFEDVKGLVADFGYI
                     ADLLGERMGCFLFQFPASFDYTPERLARIVAQLEPRRRNVVEFRHASWWNAAVHDAFR
                     DHGVIFCATSGPNLPDAVVKTADEIYVRFHGPERWYRHDYADAELADWARRIRDSGAR
                     RVWAYFNNDYNGFAIRNAETLRRMLKG"
     gene            complement(1216..>1590)
                     /locus_tag="VF402_07595"
     CDS             complement(1216..>1590)
                     /locus_tag="VF402_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX7800198.1"
                     /translation="PAVLIRNGRVFITYSASATDENYCMGLLTADENADLMDPKAWAK
                     SPQPVFRSSPEHNVWGPGHNGFTVDEKGRDMLLYHARDYREIQGEPLHDPNRSERLQY
                     IRYRADGTPDFGVPVANGPLRA"
BASE COUNT          264 a          515 c          534 g          277 t
ORIGIN      
        1 gaagccaggt gcgagccgga tcacgaactg gtctggctgg agccgatgca gacgctggcg
       61 aacctgaaaa acgaaggcta tgcgtgggcc gtgacgctgt ggctgcgcca gcgcacgcat
      121 cgggactaaa cgtctgccag cccctcaccc gaggatcggc ggcttcgcct tgctcctcgt
      181 cctctcccca tggccatggg gagaggggaa gcaataggcg gcggtttgaa cacgatttac
      241 cgtagggagc gtcgtactcg tctggcttgc gaggctgaga cagtctgcgc taacccttca
      301 gcatccgccg cagggtttcg gcgttcctga tcgcgaagcc gttataatcg ttgttgaaat
      361 aggcccagac gcgccgggcg ccgctgtcgc ggatgcgccg cgcccagtcg gccagttcgg
      421 cgtcggcata atcgtggcgg taccagcgct ccgggccgtg gaagcgcaca tagatctcgt
      481 cggcggtttt gaccaccgcg tccggcaggt tcgggccgct ggtcgcgcag aagatgacgc
      541 cgtggtcgcg aaaggcgtca tggaccgccg cgttccacca actggcgtgg cggaactcca
      601 ccacgttgcg gcgccgaggc tccagttgcg ccacgatgcg cgccagccgc tccggcgtgt
      661 agtcgaagct ggccggaaac tggaacagga agcagcccat gcgttcgccc agcaggtcgg
      721 cgatatagcc gaaatccgcg accagcccct tcacgtcctc gaaacggcgc acatgggtga
      781 tcagctcgga aaccttgacc gtatagacga aatcccgctc ccccgcctgc cgcagccagc
      841 ccttgaccgc gttcacggtc ggccagctat agaagggcgc gttcagctcg accgtgtcga
      901 accggtcggc gtaatagccg aaccagtcct gcgtcgccat ctgcgccgga tagaacaagc
      961 ccttccagtc ccagtagaac cagcccgaac agccgatatt gcaggcgggc gccgcatcgc
     1021 cgcgcggggc gccggcgtaa aggtcggcca ggcgcgcctt gtgcatcgcc tcggcgcgga
     1081 cgggattggc ggcgcgctgc ttttcgcgcc gcatcgcctt gcgggcgcgg cggtcttcgg
     1141 cggaaggctt ggcgggggcg ttcatgcgcc gaaggctgac gcgcgcggcg tcaaggttca
     1201 atgccggttt cggccttacg ccctcaacgg cccattcgcc acggggacgc cgaagtcggg
     1261 cgttccgtcg gcgcggtaac ggatatattg cagccgctcg ctgcggttcg ggtcgtgcag
     1321 cggctcgccc tgaatctcgc gatagtcgcg cgcgtggtag agcagcatgt cgcggccctt
     1381 ttcatcgacg gtgaagccgt tgtgccccgg cccccagaca ttgtgttcgg gggaggacct
     1441 gaacaccggc tgcggcgatt tcgcccacgc cttcgggtcc atcaggtcgg cgttttcgtc
     1501 cgccgtcaac aggcccatgc agtagttctc gtcggtggcg ctggccgaat aggtgatgaa
     1561 gacgcgcccg ttgcggatca gcacggcggg
//
LOCUS       DASVLL010000458         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_515316,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000458 DASVLL010000000
VERSION     DASVLL010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515316"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..878)
                     /locus_tag="VF402_07600"
     CDS             complement(57..878)
                     /locus_tag="VF402_07600"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX7800199.1"
                     /translation="MQTLAALRSQIDALDADLLRLVDARAGLGAAIAEAKARENGARE
                     NGAREGGADAQTSQLRPDREAMLIRKLLAMPRQSASDAVVVRVWRELISENLRIQGAA
                     HGGLHLNLSSQEHSREVLVWARERFGFAPSFGYVDNAAAAVQAARDPRHISVLSLDPR
                     GGAWWARLLAEPKVRVMAALPELSAGRFHAVAVAAIAPEPTGDDVTFWVSDSPQHENR
                     VIETLGHNGLAADFLCAAQGLKLFALHGFVQENDPRLGSPELGSLSGVIGAAARI"
     gene            complement(965..2011)
                     /locus_tag="VF402_07605"
     CDS             complement(965..2011)
                     /locus_tag="VF402_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEX7800200.1"
                     /translation="MSDAARTKRHRIGIFAPAVLVALLFAGWSAYWLHTAHRLEAEVA
                     KQRDALIKAGYQVSYDPFRIQGFPYRMKADFKNLSVIAPSGRGFAAGEAEAQADAYAL
                     DKWVIVVPDGFTVYRGHPGGVELGRIAVRGSALRASVSHIGKPIWNVALQGLGLSLTP
                     SDPAHPFAFSTADNFEAYLRPDAQDKDAADMLVRVTGAKGMPDSLVAGLSGGGPLSLH
                     IETTVAGMSGFQGRTFDDALKSWSAKGGRLTAFKSEVQAGTLSLYSHADALSLQGDGH
                     VAGHLELEMSGTFRPVEVLGALRIVSPENMTLARPLLDMTLATKDTQKFGIDFRDGGA
                     YIGLLKVSDAPIFP"
     gene            complement(2035..>2343)
                     /locus_tag="VF402_07610"
     CDS             complement(2035..>2343)
                     /locus_tag="VF402_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX7800201.1"
                     /translation="RLIAFDRVPWPRPDCLHRARRLHFGNKIYDDALTRARLAQAFGR
                     LIRKKDDRGVFVMLDSAAPTRLFSGLPEGVVLQRCTLAEALASIPRFLYPEEVVNNDA
                     "
BASE COUNT          412 a          789 c          771 g          371 t
ORIGIN      
        1 ttacgtgtga ggcgttcgca atccgatagg gcgcttcgcg ctcttttccg tggcgtctaa
       61 atccgcgccg ccgcgccgat cacgccggac aggctgccta attccggcga accgaggcgc
      121 ggatcgttct cctgcacgaa accgtgcagg gcgaacagct tcaagccttg cgccgcgcac
      181 aggaagtcgg cggcgaggcc gttatggccg agcgtctcga tcacgcggtt ttcgtgctgc
      241 ggcgaatccg acacccagaa ggtgacgtcg tcgccggtcg gctccggcgc gatcgccgcc
      301 accgccacgg catgaaaacg ccctgccgag agttcgggca gggcggccat gacgcgcacc
      361 ttgggttcgg ccagcagacg cgcccaccac gcgccgccgc gcgggtccag cgacaggacg
      421 gagatatgac gcggatcgcg cgcggcctgc acggcggcgg cggcgttgtc gacatagccg
      481 aagctgggcg cgaagccgaa gcgttcgcgc gcccagacca agacttcgcg cgaatgctcc
      541 tgactggaca ggttcaggtg caaaccgccg tgagccgcgc cctgaatacg caggttttcc
      601 gagatcagct cgcgccagac gcgcaccacc accgcgtccg acgccgattg gcgcggcatg
      661 gccagcagtt tgcggatcag catggcctcg cggtcggggc gcagttgcga ggtttgcgcg
      721 tccgccccgc cttcgcgcgc cccgttttcg cgcgccccgt tttctcgcgc cttggcctcg
      781 gcgatggcgg cgcccagacc ggcgcgcgcg tcgaccagac gaagcagatc ggcgtcgagc
      841 gcgtcgatct ggctgcggag agcggcaaga gtctgcaaag gaaaacactc gtaactatca
      901 atagaggcgc aagcttaggc tccgcgccgc ctgttaacaa gtgcggaaat gccagaaaac
      961 agtattaagg aaatataggc gcatctgaaa ctttcagcag gccgatatag gcgccgccat
     1021 cgcggaaatc tatgccgaac ttctgcgtgt ctttggtcgc cagcgtcatg tcgagtagcg
     1081 gccgggccag ggtcatgttc tccggcgaga cgatgcgcag ggcgcccagc acctcgaccg
     1141 gcctgaacgt gcccgacatc tccagctcca gatgcccggc gacgtggccg tcgccttgca
     1201 ggctcagcgc gtcggcgtgg ctgtagaggc ttaaagtccc cgcctgcact tcggatttga
     1261 acgcggtcag cctgccgccc ttcgcgctcc agcttttcag cgcgtcgtcg aaggtgcggc
     1321 cctgaaagcc cgacatgccg gcgaccgtgg tttcgatatg cagcgacaac gggccgccgc
     1381 cgctcagccc cgcgacgagg ctgtccggca tgcccttggc gccggtgacg cgcaccagca
     1441 tgtcggcggc gtccttgtcc tgagcgtcgg ggcgcagata ggcctcgaaa ttgtcggcgg
     1501 tcgagaaggc gaacggatgc gccgggtccg acggcgtcag gctcaggccc agcccttgca
     1561 gggcgacgtt ccagatcggc ttgccgatat gcgagacgct ggcgcgcaag gccgagccgc
     1621 gcaccgcgat ccggcccagc tcgacgccgc ccggatggcc gcgatagacc gtaaagccgt
     1681 ccgggaccac gatcacccat ttatcgagcg cataggcgtc ggcctgcgcc tcggcctcgc
     1741 ccgccgcgaa gccccgcccc gaaggcgcga tcacgctgag gttcttgaaa tccgccttca
     1801 tgcgataggg aaaaccctga atgcggaacg gatcgtagct gacctgataa ccggccttga
     1861 tcagggcgtc gcgctgtttg gcgacctcgg cttcgagacg gtgggcggtg tgcagccagt
     1921 aggcgctcca gcccgcgaac aacagcgcga ccagaacggc gggcgcgaaa ataccgatac
     1981 gatgacgctt tgtcctggcc gcgtcgctca tctcaatcct cccctcaggc cgcttcaggc
     2041 gtcgttatta accacttcct ccggataaag gaagcgcggg atcgaggcca gcgcctcggc
     2101 cagcgtgcag cgttgcagca ccacgccttc gggcaggccg gaaaacagcc gcgtcggcgc
     2161 ggcggaatcg agcataacga acacgccgcg gtcgtccttc ttgcggatca gccgcccgaa
     2221 ggcctgcgcc agccgcgcgc gcgtcagggc gtcgtcgtaa atcttattgc cgaaatgcag
     2281 cctgcgcgcg cggtgcaggc agtccgggcg cggccacggc acgcggtcga aggcgatcag
     2341 gcg
//
LOCUS       DASVLL010000459         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_138495,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000459 DASVLL010000000
VERSION     DASVLL010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138495"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..931
                     /locus_tag="VF402_07615"
     CDS             83..931
                     /locus_tag="VF402_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800202.1"
                     /translation="MRLILPAVSLLALTVMALSAPGYARVTHPAAKPRPAAKAAEKSA
                     AKPADNPAVTGRNADGALHMPVLADAPTDDFERVAWCHGVLSGDMELAEIINPVMPTD
                     DQLQLIGASYLRAYEAALTLSGKGKAESEHKKAEAARDFGYNEWAAARKAPLKEAAGL
                     YVNWQLPGECEKAAVRLSGHPNLFAEMATDEEAEAIQAVMTSGGPHDYSEKPKPVLTA
                     QAAPTDGGPVAANTLGRRVDLDAALPKIQKPAPADSDADKPKDEKRHWSEGLAYRLGW
                     SDTPKK"
     gene            complement(932..1207)
                     /locus_tag="VF402_07620"
     CDS             complement(932..1207)
                     /locus_tag="VF402_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7800203.1"
                     /translation="MQVKWSSAARRDLVRLHDFLHPVSPRAAGQTIKILIAAVDKLSA
                     YPEAAPLSDDYDGRNVRSVIVGDYNLHYEVTPDVIFIVRIWHTREDR"
     gene            complement(1209..1439)
                     /locus_tag="VF402_07625"
     CDS             complement(1209..1439)
                     /locus_tag="VF402_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX7800204.1"
                     /translation="MPSHLVAVPADLDEALDAAAQRLSRPRDWIVEQALQSWISREER
                     RHQMIEEGLKDIDEGRVIDHAEIKTWAAGLKD"
     gene            complement(1506..1715)
                     /locus_tag="VF402_07630"
     CDS             complement(1506..1715)
                     /locus_tag="VF402_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800205.1"
                     /translation="MPPSDDDIYAEKLPHPIEAPYVTQKVIRELRAMAAGEEAYARFA
                     YISPRIKRVWIVAALLIEAYQASLR"
BASE COUNT          415 a          697 c          673 g          365 t
ORIGIN      
        1 tttcggtaac accgtcaaaa aggcgatttt atgccgcagt tgcgctataa gcgcagatag
       61 aatctttctc ggagccgctc ccttgcgcct tatcctaccg gctgtttccc tgctcgccct
      121 caccgtcatg gccctcagcg cgcccggtta cgcgcgggtc acgcatccgg ccgccaagcc
      181 caggcctgcg gcgaaggcgg cggaaaagtc tgcggcgaag cccgccgaca acccggcggt
      241 gacaggccgc aacgccgacg gcgcgctgca tatgccggtg ctggccgacg cgcccacgga
      301 cgatttcgag cgcgtggcgt ggtgccacgg cgtgctgtcg ggcgatatgg agctggcgga
      361 aatcatcaat ccggtgatgc cgaccgacga tcagttgcaa ctgatcggcg cctcttacct
      421 gcgcgcctac gaggcggcgc tgaccctgtc gggcaagggc aaggccgaat ccgagcacaa
      481 aaaggccgag gcggcgcgcg atttcggcta taacgaatgg gccgccgcgc gcaaggcgcc
      541 gctgaaggaa gccgccggcc tctacgtcaa ctggcaactg ccgggcgaat gcgaaaaggc
      601 cgccgtgcgc ctgtccggcc atcccaacct gttcgccgaa atggccaccg acgaggaggc
      661 cgaggcgatt caggccgtca tgacctccgg cgggccgcac gattacagcg aaaagcccaa
      721 gcccgtgctg accgcccaag ccgcgcccac ggacggcggc cccgtcgccg ccaacacgct
      781 gggccgccgc gtcgacctcg acgccgccct gccgaaaatc cagaaaccgg cgcccgcgga
      841 ctccgacgcc gacaagccca aggacgaaaa gcgccactgg tccgagggcc tggcctaccg
      901 cctcggctgg agcgacacgc cgaagaagtg atcagcggtc ttcgcgggtg tgccagatgc
      961 ggacgatgaa gatcacgtct ggcgtcactt cgtagtgcag gttataatcc ccgacgatga
     1021 cggagcgcac gtttcgaccg tcatagtcgt cggacaaagg ggcggcttcg ggataggccg
     1081 acagcttgtc cacggcggcg atcaggattt tgatggtctg acccgccgcg cgcgggctga
     1141 cgggatgcag gaaatcgtgc agccgcacaa gatcacgccg cgccgcggac gaccatttga
     1201 cctgcatgtc agtctttcag tcctgcggcc cacgtcttta tttcggcgtg gtcgataaca
     1261 cggccttcat cgatatcctt caagccttcc tcgatcatct gatgacgccg ttcctcacgg
     1321 ctgatccagc tttgcagggc ctgttcgacg atccagtcgc gcggacgcga cagcctttgc
     1381 gccgcggcgt caagggcttc atcgagatcg gcgggaaccg cgacaagatg agagggcatg
     1441 gttggttttc cggactatgg gtcgatattg tcacccaatg gatggttttc gtcaaccgcg
     1501 cccgcttagc gcaacgacgc ctgatacgcc tcgatcagca gggccgcgac gatccagacg
     1561 cgtttgatgc gcggcgagat ataggcgaag cgcgcatagg cttcctcgcc cgccgccatc
     1621 gcgcgcaatt cgcggatgac cttttgcgtg acatagggcg cctcgatggg atgaggcagt
     1681 ttctcggcgt agatatcgtc atccgatggc ggcatcggtc ccccttttcc agcagacgat
     1741 ctccagagac gatctctgga gaccggggtg ctagaaaacc gcccaaggaa cggcgcgacg
     1801 tgttcacgca agcgctattg tattccgccg ccaccccagc caaagccgag tggcgcatcg
     1861 gattaacgag cggatgctgc tcgccttagg taaagaggtt ttctaggccc ctgagccttc
     1921 tttgtgttcg gctcaaggcc agtctaacgc acggacgcag cgaaggaaag ctttacgagc
     1981 ggcttctcgg aattgccgca ggccgcttcc cctctccccg ccagcgggga gaggacgaga
     2041 gccgagcgta agcgaaggcg atcgggtgag gggctgctcg ccggcaaagc gcaggcgccg
     2101 cctagcattc cagcccgccc ctcgcttcgc tgcggcgaga aaaaagaatc
//
LOCUS       DASVLL010000460         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_29861, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000460 DASVLL010000000
VERSION     DASVLL010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29861"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..707
                     /locus_tag="VF402_07635"
     CDS             219..707
                     /locus_tag="VF402_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800206.1"
                     /translation="MSRMTMISMAALAALVAAGPIAGAANAQNGAQNGGGWGGRGGPL
                     GMLSRYDTNGDGKISLAEYEAGRDAMFKRMDANGDGAVSFAEIDAQEQAMQQRMGDRG
                     GRGGDRMKARMDQLKAADANGDQSISADEFKAASDAEFKALDTNGDGFIDAGEAAAMM
                     KR"
     gene            744..1460
                     /locus_tag="VF402_07640"
     CDS             744..1460
                     /locus_tag="VF402_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7800207.1"
                     /translation="MPQLTLMTPEPGPKRSGAAGHGSQPPRLTVIPGSRVDSDPDEIL
                     LERMARGDAAALSAFVARKTPRLMSLGRRLLGDSAEAEDVAQETLLRTWKQAADWLPG
                     KARIDTWMHRVALNLCYDRLRRRRDTVRAEDVEIVDSTPGPSARLEQAQASQRLQRAL
                     DALPERQKVAITLCYYQELSNIDAAKLMGVSIEALESLLARARRGLKGLMQADNDVTA
                     QVKGQKQRTRAQDGRSGREV"
BASE COUNT          282 a          484 c          597 g          213 t
ORIGIN      
        1 gatccgcaca acgagccgga ggcgtttcag cgcaggctgg cgcgcgccgt gcatacgcgc
       61 cgcctcgaca cgctggagaa gaagctcaag gacatccgcc gccgtgcgag aacggcgttc
      121 gagaaaatcg tcagccccgg cgacggatag gacggcgccg cgccgtgaaa cgcgacaagg
      181 gccgccggtg tggcggtcca tttgacggga tgacgctcat gtcgcgcatg acgatgattt
      241 cgatggccgc tctggcggcg cttgtcgccg ccggtccgat cgcgggcgcg gctaatgccc
      301 agaatggcgc ccagaatggc ggcggctggg gcggtcgcgg cggtccgctg gggatgctga
      361 gccgctacga cacgaacggc gacggcaaga tcagcctggc cgaatacgag gccggacgcg
      421 acgccatgtt caagcgcatg gacgccaatg gcgacggcgc cgtcagcttc gccgagatcg
      481 acgcgcagga gcaggccatg cagcaacgca tgggcgatcg cggcgggcgc ggcggcgacc
      541 gcatgaaggc gcgcatggac cagctcaagg ccgccgacgc caatggcgac cagtcgatct
      601 cggccgatga gttcaaggcc gcctccgacg ccgagttcaa ggcgctggac accaacggcg
      661 acggtttcat cgacgcgggc gaagccgccg cgatgatgaa gcgataggcg gcgcgggccg
      721 gctgtgataa gaggatgcga accatgccgc agttgacgct catgacgccg gagcccggtc
      781 cgaagcgtag cggcgcggcg ggacatggct cgcagccgcc gcggctgacg gttattccgg
      841 ggagcagagt ggacagcgat cccgacgaga tcttgctgga gcgcatggcg cgcggcgacg
      901 ccgccgcgct gtcggcgttc gtggcgcgca agacgccgcg cctgatgtcg ctggggcggc
      961 gcctgctcgg cgattccgcc gaggccgagg acgtggcgca ggagacgctg ctgcgcacct
     1021 ggaagcaggc ggcggactgg ctgccgggca aggcgcgcat cgacacctgg atgcaccgcg
     1081 tggcgcttaa tctatgctac gaccggctgc ggcggcggcg cgacacggtg cgggccgagg
     1141 atgtcgagat cgtcgattcc acgcccggcc cgtcggcgcg gctggagcag gcgcaggctt
     1201 cgcagcggtt gcagcgcgcg ctggacgcgc tgcccgaacg gcagaaggtt gcgatcacgc
     1261 tctgttatta tcaggagttg tcgaatattg acgcggccaa gcttatgggc gtcagtatcg
     1321 aggcgctgga gagtctgctg gcgcgggcgc gccgcggact gaaagggttg atgcaggcgg
     1381 ataacgatgt taccgcgcag gtgaagggtc aaaagcaacg gacccgcgcc caggacggcc
     1441 gatccgggcg ggaggtgtag aatgaacaag cgtatggatg aaacccggtt cgcggagatc
     1501 gtatccgcct atggcgcgca ggcgcggcgc tggcccgagg ccgagcgcgc gcaggcgctg
     1561 atcttcgcgc gcgaca
//
LOCUS       DASVLL010000461         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_84159, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000461 DASVLL010000000
VERSION     DASVLL010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84159"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1066)
                     /locus_tag="VF402_07645"
     CDS             complement(131..1066)
                     /locus_tag="VF402_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX7800208.1"
                     /translation="MEPSVYVGVDVSKDRLDVYVRPSGECFSVDQSPQGIDRLVLRLR
                     ALPVVMVALEATGGYERAAAAALSVAGLPLVVVNPAQVRSFAKALGQQAKTDRIDAQV
                     IARFVEATRPQVRPLPDDSTAALADLIARRRQIVDMISVEKQRLPLSAKPVQKSIRRV
                     IKALERELAGVNGDIDAMIRASPVWRYKEDLLTSAPGVGPTVARTLLAELPELGRLDR
                     KQIAALAGLAPYARQSGQWRGKCVIGGGRKAVRKVLFLSAMTAVRRSQSLKAFYEKLL
                     SLGKPKMLALIAVERKLLTALNAMIRDKAKWCEAA"
     gene            complement(1328..2584)
                     /locus_tag="VF402_07650"
     CDS             complement(1328..2584)
                     /locus_tag="VF402_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX7800209.1"
                     /translation="MNTFFLKTGVAVAALLAALPAAAHQTRPAMSPDFQQSSEVAAQR
                     TVKIVQTGTAAQDDAQVWGDATPDVQCPDGPVDSAGWRAIPEDLRRIARPGQCYSRLL
                     VAPQVETYMDHVVTAPAHTETRVIPAVTRVVDQDVVVSPEHTVRRLVPAVTHTEMVTE
                     VVRPAGVREERIPAEYETRATHVMTQAPQQVWTRSRGIATGAALVTPDDHQPVPYRAD
                     GTLQWPGKYGDDQSVPVDRETADYLAQGSAQDVWCLDQEPGQYAERTERVETRPESVR
                     RVEIPAVTRQVEHVVVDQPEHEVEDVVPAVVEHRQAIEVVTPEHTESYEVPAVYQDVQ
                     KQRVVGQPKTVWREVLCSKNATPKVIMSIQRALAARGYQPGAIDGHLGGQTVSAMQKF
                     QADNGLPQGQVSVEAVQLLGIDLLHR"
     gene            2803..>2994
                     /locus_tag="VF402_07655"
     CDS             2803..>2994
                     /locus_tag="VF402_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7800210.1"
                     /translation="MISRYARPSAAAIWDAATKYKIWFEIEAHAATKMAELGVIPVEA
                     AHRVFGRDDDVLIDHARHGG"
BASE COUNT          504 a          982 c          989 g          519 t
ORIGIN      
        1 cctcgcggtc caaagctgtg ctttggactc gctcgacccc gaaatcatag atttcaggga
       61 ggtataagag gagacgccgc ccttatacct ccctgaacgc gcagcgtttg gggagcgact
      121 gtgttgttcg tcaagcggcc tcgcaccatt ttgccttatc tctgatcatg gcgttgagcg
      181 cggtgaggag ctttcgttcg acggcgatga gtgcgagcat tttgggtttt ccgaggctca
      241 gcagcttttc gtagaaggct ttgaggcttt ggctccgtct cacggcggtc atggcgctga
      301 ggaagaggac tttccgcacg gcttttctgc cgccgccgat gacgcatttg ccgcgccatt
      361 ggccggactg tcgcgcatag ggcgcaagtc cggccagcgc ggcgatctgt ttacggtcga
      421 gccggcccag ttcgggcagt tcggccagca gggtgcgcgc gacggtcggg ccgacgccgg
      481 gggcggaggt gagcagatct tccttgtagc gccatacggg cgaagcgcgg atcatggcgt
      541 cgatgtcgcc attgactccg gccagttcgc gctccagcgc cttgatcacg cgccggatgc
      601 ttttctgcac cggctttgcc gagagcggca ggcgctgctt ctcgacgctg atcatgtcga
      661 cgatctgacg gcgccgggcg atcagatcgg ccagcgccgc cgtgctgtcg tcgggcaggg
      721 gacggacctg cggtcgcgtc gcctcgacga agcgcgcgat gacctgcgcg tcgatacggt
      781 cggtcttggc ctgctggccc agagctttgg cgaagctgcg cacctgagcc ggattgacca
      841 ccaccaaagg caagccagcc accgacaagg ccgccgccgc cgcgcgctcg taaccgcccg
      901 tggcctccag cgcgaccatg accacaggca gggcgcgcaa acgcaaaaca agccggtcga
      961 tcccctgcgg actttgatcg acgctaaaac actcgcctga aggacgcaca tacacatcca
     1021 ggcgatcctt cgacacatcg acgccgacat aaaccgacgg ttccatcctt gacccttcct
     1081 tgcataaacg ggctcgcatc acggccctga cgactgttcg ggattgatgg aaaagcggat
     1141 gccgaccctg ctcccagcgg gctctaaacc caagggggac ccggactaac gaccgccgcc
     1201 aacatcgccc agtataaact gaacgatgga attatcttac aagggggacc gcgcgaagac
     1261 gcgcagcggc tgagatgcgg tggtggggta tctgaccttg cggcgcccca gacgcctctg
     1321 actcccccta ccgatgcagc aggtcaatcc ccagcaactg caccgcctcg accgagacct
     1381 ggccttgcgg caggccgttg tcggcctgga acttctgcat ggcggagacg gtctggccgc
     1441 cgagatggcc gtcgatggcg ccgggttgat agccgcgcgc ggccagggcg cgctggatcg
     1501 acatgatcac cttgggcgtg gcgttcttcg agcacagcac ctcgcgccag acggttttcg
     1561 gctggccgac gacgcgctgc ttctgcacgt cctgatagac cgccggaact tcataggatt
     1621 cggtgtgttc gggcgtgacc acctcgatgg cctgacgatg ctcgacgacg gcggggacga
     1681 cgtcctcgac ctcgtgttcg ggctggtcga ccacgacgtg ttcgacctga cgggtgacgg
     1741 cggggatttc gacgcggcgc accgattccg ggcgcgtctc gacgcgctcg gtgcgctcgg
     1801 cgtactggcc cggttcctga tccagacacc agacgtcctg cgccgagcct tgcgcgagat
     1861 agtccgccgt ctcgcggtcg accggcacgg actggtcgtc gccgtacttg cccggccatt
     1921 gcagcgtgcc gtcggcgcgg tacggcacgg gctggtgatc gtccggcgtg accagggccg
     1981 cgccggtggc gatgccgcgg ctgcgggtcc agacctgctg cggcgcctgg gtcatcacgt
     2041 gcgtggcgcg cgtctcgtat tccgccggaa tacgctcctc gcgcacgccg gccgggcgga
     2101 cgacctcggt gaccatctcg gtatgggtga cggcgggcac gaggcggcgc accgtgtgtt
     2161 cgggcgagac gacgacgtcc tgatcgacca cgcgcgtcac ggcgggaatc acgcgcgtct
     2221 cggtatgggc gggcgcggtc acgacatggt ccatataggt ctcgacctgc ggcgccacca
     2281 gcaggcgcga atagcactga cccggccggg cgatgcggcg cagatcctcc gggatggcgc
     2341 gccagcccgc cgagtcgacc ggcccgtccg ggcactgcac gtccggcgtg gcgtcgcccc
     2401 aaacctgcgc gtcgtcctga gccgccgtgc ccgtctgcac gatcttgacc gtgcgctgcg
     2461 ccgcgacctc cgaggattgc tggaagtcgg gcgacatcgc cggacgggtc tgatgcgcgg
     2521 cggcgggcag ggccgccagc agggcggcga cggcgacgcc ggttttcagg aaaaacgtat
     2581 tcacgacaat aacctcagga cttcggacag cgaacgattc cggtgaaaat taacccttta
     2641 taaacctttg cgccaagtcc gcacacctga gacgggtaaa ttaaccacat tgcgtaaggg
     2701 gcgcgtaaag gcgggcgcgc agaggattag ggcttgacat tgccgcccgc gctttccatt
     2761 gccagcggcg aaactccatc caccgtccga aggcttccgc ccatgatctc ccgttacgcc
     2821 cgcccgtccg ccgccgccat ctgggacgcg gcgaccaagt acaagatctg gttcgagatc
     2881 gaggcccacg ccgccaccaa gatggccgag ctgggcgtga tcccggtcga ggcggcgcac
     2941 cgtgtgttcg ggcgagacga cgacgtcctg atcgaccacg cgcgtcacgg cggg
//
LOCUS       DASVLL010000462         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_515534,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000462 DASVLL010000000
VERSION     DASVLL010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515534"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VF402_07660"
     CDS             complement(<1..845)
                     /locus_tag="VF402_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7800211.1"
                     /translation="MTKVLKVGVAGAGVFGGYHANKYRQLPGLEFVGVYDLDPARAEA
                     LAAQHGVAAFGGGALDDFLGRLDAVTIAAPAFAHADIALKALERGVHVYTEKPLAALS
                     EDGARMADLAERKGLVLACGHQERAVFEAIGLYGVPERPSRLEAVRNGTASTRNLDVS
                     VVLDLMIHDLDLAISLAGGQPGGLSASARYREAEGYDAVGADEALADVDFDNGMKAIF
                     RASRMAEARERTMRVVYPQGEVRIDFLNRTFENTTPFALNPDFAEAEIARDPLGTSVS
                     RFLAAV"
     gene            1049..>1804
                     /locus_tag="VF402_07665"
     CDS             1049..>1804
                     /locus_tag="VF402_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiG"
                     /protein_id="HEX7800212.1"
                     /translation="MASRLFGAYVVVDYSAAEGKKTGESSMWIGVMKRDVRFRLSYEA
                     HNPAARAEGLALLREILADLHRRGDRVLLCVNFPLGFPRGSARRLGLSGEAWSALWDF
                     LGKNIVDKADNSNNRFQVAAKMNRLMTDAAYPFWGCPKSAAQKWLASVKPESYGDFPE
                     FRLTEESLRARKLPASVLPRSLWQMHGAGVAGGTAMLGIAALRALRADLGDAARLWPF
                     ETGFGALDLEALDGVTTLIAETHPPAFGGKPEPG"
BASE COUNT          286 a          622 c          605 g          291 t
ORIGIN      
        1 acggcggcga ggaagcgcga cacgctggtc cccagcggat cgcgcgcgat ctcggcctcg
       61 gcgaagtcgg gattgagcgc gaacggcgtc gtgttctcga aggtccggtt gaggaagtcg
      121 atccgcacct cgccctgcgg atagacgacg cgcatggtgc gttcgcgcgc ctcggccatg
      181 cgcgaggcgc ggaagatcgc cttcatgccg ttgtcgaaat cgacatcggc cagcgcttcg
      241 tcggcgccga ccgcgtcata gccctcggcc tcgcgatagc gcgcgctggc cgacaggccg
      301 cccggctggc cgcccgccag cgaaatggcc agatcgagat cgtggatcat caggtccagc
      361 accaccgaca cgtcgagatt gcgcgtcgac gccgtgccgt tgcgcaccgc ctccagccgc
      421 gaagggcgct ccggcacgcc gtaaagcccg atcgcctcga acacggcgcg ctcctgatgg
      481 ccgcaggcca ggaccagccc cttgcgctcg gccagatccg ccatccgcgc gccgtcctcg
      541 ctcagcgcgg ccagcggttt ttcggtatag acatgcacgc cgcgctccag cgccttcagc
      601 gcgatatcgg cgtgcgcgaa agcgggcgcg gcgatggtca ccgcgtcgag gcggccgaga
      661 aaatcgtcca gcgcgccgcc gccgaacgcc gccacgccgt gctgcgccgc cagcgcttcg
      721 gcgcgcgccg gatcgagatc gtaaacgccg acgaattcca gaccgggcag ttggcgatac
      781 ttgttcgcat gataaccgcc gaacacgccc gcgcccgcca cgccgacctt taagaccttc
      841 gtcatcgtct gcctcctgaa accggacccc tgcttagccc gcttcgcgaa caaaattcaa
      901 agcctaaatt gtgcgccgaa attgtgcaaa ggcgaagcgc gcgggcatta tgcccttttt
      961 cggcgccggc aatcgcgcta gaaacgcggc cgtctttcgc gcgcggccgc tgctgaacgc
     1021 cgcgcgcctt tccttatgag gttctttcgt ggcatcccgt ctgtttggcg cttacgtcgt
     1081 ggtcgattac agcgccgccg aaggcaagaa gaccggcgag tcgtccatgt ggatcggcgt
     1141 gatgaagcgc gacgtgcgct ttcgcctgtc gtatgaggcg cacaatccgg cggcgcgcgc
     1201 cgaggggctg gcgctgctgc gcgagattct ggccgacctg caccggcgcg gcgaccgcgt
     1261 gctgctgtgc gtcaacttcc cgctcggctt cccgcgcggt tcggcgcggc gcctgggcct
     1321 gagcggcgaa gcgtggtcgg cgctgtggga cttcctcggc aagaacatcg tcgacaaggc
     1381 cgacaacagc aacaaccgct ttcaggtcgc ggccaagatg aaccggctga tgaccgacgc
     1441 ggcctacccg ttctggggct gtccgaaatc ggcggcgcag aagtggctgg cctcggtcaa
     1501 gccggaatcc tatggcgatt ttcccgaatt tcgcctgacc gaggagtctt tgcgcgcgcg
     1561 caagctgccg gcgtcggtcc tacccaggag cctgtggcag atgcacggcg cgggcgtggc
     1621 gggcggcacg gccatgctgg ggatcgcggc gctgcgcgcc ttacgcgccg atctgggcga
     1681 tgcggcgagg ctatggccgt tcgagacggg cttcggcgcg ctcgaccttg aggcgctgga
     1741 cggcgtgacc acgctgatcg ccgagacgca cccgccggcg ttcggcggca agcccgaacc
     1801 cggc
//
LOCUS       DASVLL010000463         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_139140,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000463 DASVLL010000000
VERSION     DASVLL010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139140"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1554
                     /locus_tag="VF402_07670"
     CDS             <1..>1554
                     /locus_tag="VF402_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7800213.1"
                     /translation="ADGIEVYTTRPDTLFGAAFLAIAADHPLARAAAERDPALAGFIA
                     GLNRGGVSQADIDTAEKQGFDTGLRVKHPFIDGQTLPVWVANFVLMDYGTGAIFGCPA
                     HDQRDLDFARKYGLPVREVVLPPGADAETFAVGKEAYTGPGHIFRSGFLDGLEIEAAK
                     DAAIDRIEAMGLGEGATVFRLRDWGVSRQRYWGCPIPVIHCEACGVVPVPDDQLPVTL
                     PEDVTFDVPGNPLERHPAWKHVACPCCGRDARRETDTLDTFVDSSWYFARFTDPTAAE
                     PINRTAADYWLPVDQYIGGVEHAVLHLLYARFITRALNHCGYLDVKEPFAGLFTQGML
                     THETYKDAQGQWVEPGDVEIETVDGRRAAKRLSTGEALTIGDVEKMSKSKKNTVAPED
                     IFDTYGVDSARLFVLSDSPPERDVQWSTSGVEGSWRFTHRVWDLFDSLPDAEVPCAND
                     AVALDLRKAAHKAVKAVTEGFESFRFNAAIAKLYELVTTIRVNDVSVVGSQARRDVLE
                     LLAGLIAPVT"
BASE COUNT          251 a          569 c          486 g          248 t
ORIGIN      
        1 gccgacggga tcgaggtcta caccacccgc ccggacacgc tgttcggcgc cgccttcctc
       61 gccatcgccg ccgatcatcc gctggcgcgc gccgccgccg aacgcgatcc cgccctcgcc
      121 ggctttatcg ccggcctcaa ccggggcggc gtgtcgcagg ccgatatcga caccgccgaa
      181 aagcagggct tcgacaccgg cctgcgcgtc aagcacccgt tcatcgacgg tcagaccctg
      241 cccgtctggg tcgccaactt cgtgctgatg gactacggca cgggcgccat tttcggctgc
      301 ccggcgcatg accagcgcga cctcgatttc gcgcgcaaat acggcctgcc ggtgcgcgaa
      361 gtcgtgctgc cgcccggcgc cgacgccgaa acctttgcgg tgggcaagga agcctatacc
      421 ggccccggcc atatcttccg ctccggcttc ctcgacgggc ttgagatcga ggccgccaag
      481 gacgcggcca tcgaccgcat cgaagcgatg ggcctcggcg agggcgcgac cgtgttccgc
      541 ctgcgcgact ggggcgtgtc gcgccagcgc tactggggct gcccgatccc ggtcatccat
      601 tgcgaggcgt gcggcgtggt ccccgtgccg gacgatcagt tgccggtcac cttacccgaa
      661 gacgtgacct tcgacgtccc cggcaatccg ctggagcgtc atccggcctg gaagcacgtc
      721 gcctgtccgt gctgcggccg ggacgcgcgg cgcgaaaccg acacgctcga caccttcgtc
      781 gattcctcgt ggtatttcgc gcgcttcacc gacccgaccg ccgccgagcc gatcaacagg
      841 accgccgccg actactggct gccggtcgat cagtatatcg gcggcgtcga acacgcggtc
      901 ctgcaccttc tgtatgcgcg cttcatcacc cgcgcgctga accattgcgg ctatctcgac
      961 gtcaaggagc cgttcgcggg cctgttcacg caaggcatgc tgacgcacga gacctataag
     1021 gacgcgcaag gccagtgggt cgaacccggc gatgtcgaga tcgaaaccgt cgacggccgc
     1081 cgcgccgcca aacgcctgtc caccggcgag gccctgacca tcggcgacgt cgagaagatg
     1141 tcgaaatcga agaagaacac ggtcgcgccg gaagacatct tcgataccta tggcgtcgat
     1201 tccgcccgcc tgttcgtgct cagcgactcg ccgcccgaac gcgacgtgca atggtcgacc
     1261 tccggcgtcg aaggctcgtg gcgcttcacc caccgcgtct gggatctgtt cgacagcctg
     1321 cccgacgcgg aggttccgtg cgccaatgac gccgtcgcgc tcgacctgcg caaggccgcg
     1381 cacaaggcgg tcaaggccgt caccgaaggc ttcgaaagct tccgcttcaa cgccgccatc
     1441 gccaagctgt acgagctggt gaccaccatc cgcgtcaacg acgtctccgt cgtcggctcg
     1501 caggcgcgcc gcgacgtgct ggagctgctg gccgggctga tcgcgccggt cacg
//
LOCUS       DASVLL010000464         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_84368, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000464 DASVLL010000000
VERSION     DASVLL010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84368"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF402_07675"
     CDS             <1..612
                     /locus_tag="VF402_07675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7800214.1"
                     /translation="HRAGVIHRDVKPENIILTGDGGFKLVDLGVSRIPDMDRDTVFVP
                     GTPSYKAPEQFEGRPGDARSDIFAMDVTLYRMFTGAYPFGEIETGQTPVFSRPAPLLE
                     KRPDLPAWLDSVILQALAPDPDARIQDGFEMAFKLETGAHGAAPDFFRKHSWLARHQV
                     GLWRGISAALLVALVFFVWAWATHWTAPEGLTRLLARIADPNG"
     gene            813..3437
                     /locus_tag="VF402_07680"
     CDS             813..3437
                     /locus_tag="VF402_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7800215.1"
                     /translation="MGFLRKALLAACLLLTPAAAHAADIPQGLLPDMATPLAYRLDLT
                     VLPDRARFSGHAEIDIDLKAATTSLYMHGRDLNVTRAAVRIGNRTLPASFRQVDPLGV
                     ARLDVASTLPKGRATLIFDYDAPFGNGPAGLYHIKVGGDWYAWTQFESIDARAAYPSF
                     DQPGYKTPFTVSLTTKPGYVAVSNAPESGEAAAGDLVRHRFETTKPLPTYLVAFAVGP
                     FVTRSGAVPPSPQRPYPLPLRVIATKPNAGRLDYVMNETPSIVAHLEAYFDRPFPFPK
                     LDQIASPVMPGAMENAGADTYEDNLILLDPGADTHQRQNFGMVVSHELSHQWFGDYVT
                     PAWWDDIWLNESFANWMGYRIGNAWRPELNIGVGAIGEAFDAMDVDALKVGRPIHQPI
                     ASNGQIDAAFDRITYGKGGQVVAMIAAYLGDATFRDGVRLHMARHPYGTADTEQFFGA
                     LADAANEPRVLDAMKSFVDQQGFPTVAISHDGDKLVATQSRYARLGTRLAPQIWTIPL
                     CVRRGETRACTLMDRPRQTLDLPKSGPIMPNAGGAGYYRFTLTPQDWETLIQAGAGLP
                     AGEGLAAADSLWGQFEAGGGSAARLIEAARVFAANPDSNVAVRLGGNLADLRRRGVIP
                     DAAQSDYQRVMEEIYQPRLAALGFDPRAGLYLSEAPDRQRLRQELVGLLAAEARDSAV
                     RHRLNAAATAYLNGDAGALDQAFFGPAFAVYVAEGGLPAAQDMYARVLHAGDAVFRAA
                     ALKAVTADATPQTARWVFASFDDGRLRASDRLALIGGLFRAPAARGLAYDWLKAHFGE
                     LTAGAGIFDAGRVASLPSDDCSAARADEIDAVMRAGVEAAGRGALSFDRMLEDMRDCG
                     ALRDAEGAHLAAALKDAP"
     gene            3587..4798
                     /locus_tag="VF402_07685"
     CDS             3587..4798
                     /locus_tag="VF402_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX7800216.1"
                     /translation="MSDTEPTPPSVSRGLPPGLIERAVNIILRPLPTWETIAGESASV
                     KSIYMGYAAILAAIGPVCHAVGGMIFGRGVPGLMVLHTPPLAAVAEAVVGYVLNLVMV
                     YVLALIIDALAPSFGGRKDRLSAFKVAAYSSTAAWVAGVFGLIPALAILGLLGFYSFY
                     LLYLGLPRLMQAPADKGLGYTVVVIIVSILLWIIVAAVGGAIGGAAMMGLGGAALTQP
                     EAQGRLTTPVGGVDLGKLQKAADQMSKQAEAMKNGTAAPVKVADPSALAALLPASYMG
                     VSAIGPSTSSGGAGGVKVAAAETSYPVNGGTIRLKISDNGGLGGLGGVMAAADMNVSN
                     SSAGGYDKLTTQNGEMVSEKYDANARHGEYAVIADGRYSITAEGDNVDMSALKSLVAG
                     IDMNRLKALGG"
     gene            complement(4779..>5123)
                     /locus_tag="VF402_07690"
     CDS             complement(4779..>5123)
                     /locus_tag="VF402_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 family protein"
                     /protein_id="HEX7800217.1"
                     /translation="AAVWFRSFIFFRVDHKTRSMPFLLANCLSAFFLWVAENIGAYTN
                     TWRYPGKDWHIVAWQKMGAWALLLVLAFVTVTLVVRPQAPESEAERAGGYRLWRRGFQ
                     RRPTPDISRPEP"
BASE COUNT          801 a         1733 c         1768 g          821 t
ORIGIN      
        1 catcgggcgg gcgtcatcca ccgcgacgtc aagccggaga acatcatcct gaccggcgac
       61 ggcggcttca agctggtcga cctcggcgtg tcgcgcattc ccgatatgga cagggacacg
      121 gtcttcgtgc cgggcacgcc cagctataag gcgccggagc agttcgaggg gcgaccgggc
      181 gacgcgcgct ccgacatctt cgcgatggac gtgacgctct accgcatgtt caccggcgcc
      241 tatccgttcg gcgagatcga gacgggccag acgccggtgt tttcgcgccc cgcgccgctg
      301 ctggaaaagc gcccggacct gcccgcctgg ctggacagcg tgatcctgca agcgctggcg
      361 cccgatcccg acgcgcgcat tcaggacggc ttcgagatgg ccttcaagct ggagaccggc
      421 gcgcacggcg cggcgccgga cttcttccgc aagcattcgt ggctggcgcg ccatcaggtc
      481 gggctgtggc gcgggatcag cgcggcgctg ctggtcgcgc tggttttctt cgtctgggcg
      541 tgggcgacgc attggacggc gccggaagga ttgacgcggc tgctggcgcg gatcgccgat
      601 ccgaacgggt aggggcgtcg aagtccccct tccgtcattt gcttcgcaaa tgctgccttc
      661 cccgtgaacg gggaaggcga aagaaactcg ccctccctgc gcagcaaggg tgggaggggc
      721 cttccatccg cagattacaa aaagttaata ttagccccgc gcacagacat gttagccgaa
      781 aggaatagtt gcaaccgtat cggacgcccg acatgggttt cctgcgcaag gccttgctgg
      841 ccgcctgcct gctcctgacg cccgccgccg cgcacgccgc cgacattccg cagggcctgc
      901 tgcccgatat ggcgacgccg ctggcctacc ggctcgacct gaccgtgctg cccgaccggg
      961 cgcgtttctc cggccacgcc gagatcgaca tcgacctgaa ggccgcgacg acgtccctct
     1021 acatgcatgg ccgcgacctg aacgtcaccc gcgcggcggt cagaatcggc aacaggacgc
     1081 tgcccgcaag tttccgtcag gtcgatccgc tcggcgtggc gcggctcgat gtcgcctcga
     1141 ccctgcccaa gggccgggcg accctgatct tcgactatga cgcgccgttc gggaacggtc
     1201 cggcggggct ttatcacatc aaggtcggcg gcgactggta cgcctggacg cagttcgaat
     1261 ccatcgacgc gcgcgccgcc tatccgtcct tcgaccagcc cggctacaag acgccgttca
     1321 ccgtgtcgct gacgacaaag cccggctatg tcgccgtctc caacgcgccg gaaagcggcg
     1381 aggccgcggc gggcgatctg gtccggcaca ggttcgagac gaccaagccg ctgccgacct
     1441 atctggtcgc cttcgcggtc gggccgttcg tgaccaggag cggcgccgtc ccgccgtcgc
     1501 cgcagcgccc gtatccgctg ccgctgcgcg tgatcgcgac caagccgaac gcgggcaggc
     1561 tcgactatgt gatgaacgag acgccgtcca tcgtcgcgca tctcgaagcc tatttcgacc
     1621 ggcccttccc gttcccgaag ctggaccaga tcgcttcgcc ggtcatgccg ggcgcgatgg
     1681 agaatgcggg cgcggacacc tatgaggaca atctcatcct gctcgatccg ggcgccgaca
     1741 cgcatcagcg ccagaatttc ggcatggtgg tctcgcacga gctgtcgcac caatggttcg
     1801 gcgattacgt cacgcccgcc tggtgggacg atatctggct gaacgagagc ttcgccaact
     1861 ggatgggcta tcgcatcggc aacgcctggc gcccggagct gaacatcggc gtcggcgcca
     1921 tcggggaggc tttcgacgcg atggacgtcg acgcgctgaa ggtcgggcgc ccgatccatc
     1981 agccgatcgc gtcgaacggc cagatcgacg ccgccttcga ccgcatcacc tatggcaagg
     2041 gcggtcaggt ggtggcgatg atcgccgcct atctcggcga cgcgaccttc cgcgacggcg
     2101 tgcggctgca tatggcgcgc catccctacg gcacggcgga cacggagcag ttcttcggcg
     2161 cgctggccga cgcggccaac gagccgcgcg tgctggacgc gatgaaaagc tttgtcgatc
     2221 agcagggctt tcccaccgtg gccatcagcc atgacggcga taaactggtc gcgacccaga
     2281 gccgctatgc gcgccttggg acgcggctcg cgccgcagat ctggacgatt ccgctctgcg
     2341 tccggcgcgg cgaaacgcgc gcctgcacgc tgatggaccg gccccgccag accctcgacc
     2401 tgcccaaaag cggcccgatc atgccgaacg cgggcggcgc gggttattac cgcttcacgc
     2461 tgacgccgca ggactgggaa acgctcatcc aggcgggcgc cggtctgccg gcgggcgaag
     2521 ggctggccgc cgccgacagc ctgtggggcc agttcgaggc gggcggcgga agcgccgcgc
     2581 gactgatcga ggcggcgcgg gtcttcgcgg ccaatcccga ctccaacgtc gcggtcaggc
     2641 tgggcggcaa tctggccgac ctgcgccgcc gcggcgtgat tcccgacgcc gcgcaaagcg
     2701 attaccagcg cgtgatggag gaaatctatc agccgcgcct ggccgcgctc ggcttcgatc
     2761 ccagggcggg cctctacctg tcggaggcgc cggaccgtca gaggctgcgt caggaactgg
     2821 tcgggctgct ggccgccgag gcgcgcgaca gcgccgtgcg ccaccggctg aacgccgccg
     2881 cgacggccta tctgaacggc gacgcgggcg cgctggatca ggccttcttc ggccccgcct
     2941 tcgccgtgta cgtcgccgag ggcggcctgc ccgccgcgca agacatgtat gcgcgcgtgc
     3001 tgcacgcggg cgacgcggtg ttccgcgccg ccgcgctcaa ggcggtgacg gcggacgcga
     3061 cgccgcaaac ggcgcggtgg gtgttcgcca gcttcgacga cgggcgcctg cgcgcctcgg
     3121 accggctggc cctgatcggc ggcctgttcc gggcgcccgc cgcgcgcggt ctggcctatg
     3181 actggctgaa ggcgcatttc ggcgagctga cggcgggcgc gggcattttc gacgccgggc
     3241 gcgtcgcgtc tctgccgtcg gacgactgtt ccgccgcccg cgccgacgag atcgacgcgg
     3301 tcatgcgcgc cggggtcgag gccgccgggc gcggcgctct gtccttcgac cggatgctgg
     3361 aggacatgcg cgactgcggc gcgctgcggg acgccgaggg cgcgcatctg gcggcggcgc
     3421 tgaaagacgc gccgtaacag accggtatcg cgattgtgac aggcggaggc gtccggttcg
     3481 attgaccgga cgcctttttt tggacatttt tccccgttcg cgcggtttcg ccgcctgtac
     3541 cggtatgcat attttttcac gtccgctctt tttcgggaaa gccctcatgt ccgatacaga
     3601 acccacgccg ccctcggttt cgcgcggcct gccgcccggc ctgatcgagc gcgcggtcaa
     3661 catcatcctc aggccgctgc cgacatggga gacgatcgcg ggcgaaagcg ccagcgtgaa
     3721 atccatctat atgggctatg cggcgattct ggcggccatc ggtccggtct gccatgcggt
     3781 cggcggcatg atattcggac gcggcgttcc gggcctgatg gtgctgcaca cgccgccgct
     3841 ggcggcggtt gcggaggcgg tcgtcggcta tgtgctgaac ctcgtcatgg tctatgtgct
     3901 ggcgctgatc atcgacgcgc tggcgcccag tttcggcggg cgcaaggaca ggttgtcggc
     3961 gttcaaggtc gcggcctatt cgtcgacggc ggcgtgggtc gcgggcgtgt tcggcctgat
     4021 accggcgctg gcgattctgg gcctgctcgg cttttacagc ttctatctgt tgtatctggg
     4081 gctgccgcgc ctgatgcagg cgcccgccga caaggggctg ggctatacgg tggtggtcat
     4141 catcgtgtcg attctgttgt ggatcatcgt ggccgccgtc ggcggcgcga tcggcggcgc
     4201 ggcgatgatg gggctgggcg gcgcggcgct gacgcaaccg gaggcgcagg gcaggctgac
     4261 cacgccggtc ggcggcgtcg atctcggcaa gctgcaaaag gccgccgacc agatgtcgaa
     4321 acaggccgag gcgatgaaga acggaaccgc ggcgccggtg aaggtcgccg acccctcggc
     4381 cctggccgcc ctgctgcccg ccagctatat gggcgtctcg gcgatcggcc cctcgacctc
     4441 gtcgggcggc gcgggcggcg tcaaggtcgc ggcggcggaa accagctatc cggtcaacgg
     4501 cggcacgatc cgcctcaaga tcagcgacaa cggcgggctg ggcggcctcg gcggcgtcat
     4561 ggccgccgcc gacatgaacg tcagcaacag cagcgcgggc ggctacgaca agctgacgac
     4621 gcagaacggc gagatggtca gcgaaaaata cgacgccaac gcgcgccacg gcgaatacgc
     4681 ggtgatcgcc gacggccgct acagcatcac cgccgagggc gacaatgtcg acatgtcggc
     4741 gctgaaatcc ctcgtcgccg ggatcgacat gaaccggctc aaggctctgg gcggctgatg
     4801 tccggggtcg ggcggcgctg aaatccgcgc cgccacaggc gatagccgcc ggcgcgttcg
     4861 gcctcgcttt ccggcgcctg cggcctgacc accagcgtca ccgtcacgaa ggccagcacc
     4921 agcagcaagg cccacgcgcc catcttctgc caggcgacga tatgccagtc cttgcccgga
     4981 tagcgccatg tgttcgtata ggcgccgata ttctccgcca cccacagaaa gaaggcgctg
     5041 agacagttgg ccagcaggaa cggcatggac cgggtcttgt ggtcgacgcg gaagaagatg
     5101 aagctcctga accagacggc ggc
//
LOCUS       DASVLL010000465         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_515835,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000465 DASVLL010000000
VERSION     DASVLL010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515835"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /gene="plsX"
                     /locus_tag="VF402_07695"
     CDS             <1..1042
                     /gene="plsX"
                     /locus_tag="VF402_07695"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX7800218.1"
                     /translation="RRPDLFFQLHGDPLLINPVLARHPELKARATVVETDQFIAMDEK
                     PAQAIRRRNTSLANAIHAVKAGEAKAAVSAGNTGALMALSKMILKMITHDLERPAIGC
                     LWPNAKGYSTVLDVGANVTSDARQLIEFAMMGAAFHRAVKGVDKPSIGLLNIGSEDVK
                     GHEEVREAHRLLRDNAFGLDYYGFVEGTDLCAGTVDVIVTDGFTGNVALKTAEGTARF
                     IRDLLRGALKSSPLAMLGALLAAPALKAMAKKVDPGASNGGPLLGLNGVVIKSHGGAD
                     ARAYANAIAVAASLAGSGYARELESSLALLTRSLADGDGDGGLAVTGNDTNIEAKTPA
                     VMAVAETENKVS"
     gene            1047..>1706
                     /locus_tag="VF402_07700"
     CDS             1047..>1706
                     /locus_tag="VF402_07700"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX7800219.1"
                     /translation="MRVLRSAVTGVGGYLPETVMPNTDFEKLVDTSDAKIVELTGIRQ
                     RHRVAEGQTTSDLAAEAAKVALAAAGKSAADVDMIVVATTTPDMPFPSTASLVQKKIG
                     APVCPAFDVQAVCSGFVYALSVADGFVARGLAKCVLVIGAEAMTRLLDFSDRSTCILF
                     GDGAGAVVLEPVEGEGTTDDRGILGFDLRCDGSKTDLLYVSGGVFEADNRIGKIKMQG
                     NQ"
BASE COUNT          294 a          527 c          615 g          270 t
ORIGIN      
        1 gcggcggccg gacctgttct ttcagttgca tggcgatccg ttgctgatca acccggtgct
       61 ggcgcgtcat cctgagttga aggcgcgcgc gacggtggtc gaaacggacc agttcatcgc
      121 catggacgaa aaaccggcgc aggcgatccg ccgccgcaat acgagtctcg ccaacgccat
      181 tcacgccgtg aaggcgggcg aggcgaaggc ggcggtgtcg gcgggcaaca ccggcgcgct
      241 gatggcgctg tcgaagatga tcctgaagat gatcacccac gatctggagc gcccggccat
      301 cggctgtttg tggccgaacg ccaaggggta ctccaccgtg ctggacgtcg gggcgaacgt
      361 gacctcggac gcgcggcaac tgatcgaatt cgccatgatg ggcgcggcct ttcaccgcgc
      421 ggtgaagggc gtcgacaagc ccagcatcgg cctgctgaac atcggctccg aggacgtcaa
      481 ggggcatgag gaggtgcgcg aggcgcaccg gctgctgcgc gacaacgcct tcggtctgga
      541 ctattacggc ttcgtcgagg gcaccgatct gtgcgcgggc acggtcgacg tgatcgtcac
      601 cgacggcttc accggcaatg tggcgctcaa gaccgccgag ggcacggcgc gcttcatccg
      661 cgacctgctg cgcggcgcgc tgaaatcgtc gcctctggcc atgctgggcg cgctgctggc
      721 ggcgcccgcg ctgaaggcga tggcgaagaa ggtcgatccg ggcgcctcga acggcgggcc
      781 gctgctgggg ctgaacggcg tggtgatcaa gagccacggc ggcgccgacg ccagggccta
      841 cgccaacgcc atcgcggtgg cggcgtcgct ggccggcagc ggctatgcgc gcgaactgga
      901 aagctcgctg gcgctgctga cgcgcagcct ggcggacggc gacggcgacg gggggcttgc
      961 tgtaaccgga aatgatacaa atatagaggc gaagacgccc gccgtgatgg cggttgccga
     1021 gacggaaaac aaggtaagtt gaaactatgc gtgtgttgag atcggcggtc acgggcgtgg
     1081 gcggctacct gcccgaaacg gtgatgccca atacggattt cgagaaactg gtcgacacct
     1141 ccgacgccaa gatcgtcgag ctgaccggca tccgccagcg ccatcgcgtc gcggaaggcc
     1201 agacgacgtc cgatctggcc gccgaggccg cgaaggtcgc tttggccgcc gccgggaaga
     1261 gcgccgccga cgtggatatg atcgtcgtcg cgacgaccac gcccgacatg ccgtttccgt
     1321 ccaccgcctc gctggtgcag aagaagatcg gcgcgcccgt gtgtccggcc ttcgacgtgc
     1381 aggcggtgtg ttcgggcttc gtttacgccc tgagcgtggc cgacggcttc gtggcgcgcg
     1441 ggctggccaa atgcgtgctg gtgatcggcg ccgaggcgat gaccaggctg ctcgatttct
     1501 ccgaccgttc gacctgcatc ctgttcggcg acggcgccgg ggcggtggtg ctggagccgg
     1561 tcgagggcga ggggacgacg gacgatcgcg gcatactggg cttcgacctg cgctgcgacg
     1621 gctccaagac cgaccttctg tacgtttccg gcggcgtgtt cgaggcggac aaccgcatcg
     1681 gcaagatcaa aatgcagggc aatcag
//
LOCUS       DASVLL010000466         4739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_300339,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000466 DASVLL010000000
VERSION     DASVLL010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300339"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VF402_07705"
     CDS             <1..174
                     /locus_tag="VF402_07705"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478048.1"
                     /note="catalyzes the formation of N-acetyl-l-glutamate
                     5-semialdehyde from 2-oxoglutarate and
                     N(2)-acetyl-L-ornithine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX7800220.1"
                     /translation="RDVMTIARDNGLLIAGGSDNCIRLLPPLNLTEDEAREAIGLLDK
                     TFAEAREKLKESA"
     gene            174..1091
                     /gene="argF"
                     /locus_tag="VF402_07710"
     CDS             174..1091
                     /gene="argF"
                     /locus_tag="VF402_07710"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7800221.1"
                     /translation="MKHFLDICDLTPADLRAILDDAHARKAARRGWPKGRVDADAPAQ
                     DRVLAMIFQKNSTRTRFSFDAAIRQLGGDAIISTASDMQLGRGETIEDTARVLSRMVD
                     AIMIRANEHDDVVRLARASTVPVINGLTNLSHPCQIIADLQTIEEHRGNIAGKKLAWI
                     GDGNNMCTSFIHAAARFGFELAIACPDAYRPNPQALVFASESRARVTVSNDPAAAARD
                     ADVVLADTWVSMGDLDHDERLAAFQPYQVNAGLMKLAKPDALFLHCLPAHRGEEVTDD
                     VIDGPQSVVWDEAENRIHAQKAVIAWCFS"
     gene            complement(1228..2511)
                     /locus_tag="VF402_07715"
     CDS             complement(1228..2511)
                     /locus_tag="VF402_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEX7800222.1"
                     /translation="MTQMTMSPTTLRADPARVVILPFSISIMPRDSAMGAMSRVTRIC
                     AALTALSSEQCRAELEVVNRDFTGRHWQTRGIFLDRFRQIQQMQGDMENLDEDHQALI
                     GAYFCHEYSYQAAAIMNPSVVPHPDQSNANGGVNFVMSARTVGEGHTSTISFREGVFY
                     PDGTMELWPEADFAVAAKPDLNAPLDGAVEVRGHQACPISETVLFPVTRAQSNGLEDL
                     RLTWFEDEDGSRAYIGTYTAYSGRETACECLETQDFRAFRLTPFTGAASQHKGLAFFP
                     RRMNGRYAAIARLDHESLFYLESDDRYAWNNGERIMGPEAPWELIQMGNCGSPIELDE
                     GWLLLTHGVGAVRRYSLGAALLDKDDPRKLIARSKTPLLSPSEDNREGYVPNVIYTCG
                     AMRVGDWIFMPYGVADSSIHFASIHVKDMLAHLGV"
     gene            complement(2536..>4739)
                     /locus_tag="VF402_07720"
     CDS             complement(2536..>4739)
                     /locus_tag="VF402_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7800223.1"
                     /translation="AIIGNFPPRQCGLATFTRDMYACLSKALPDAAWRVIAMNDAPDA
                     GYAYPDAVTDAIFQDDQAAYAQLARSLREDGTEALFVQHEFGIYGGPSGAYLLDLLEA
                     VDMPIVTTLHTVLARPTPEQRRVMEGLIRLSTTLVTMARKGAEILKTVYGVPDGQIAV
                     VPHGAPSRPLRDTAAFKERMGLSGRRTLTTFGLLSPNKGVETVIAALPELARQAPDLV
                     YLVIGATHPHLIRHEGEAYRERLKAMARDLGVEDHVRFIDRFIGDDELIDILQATDIY
                     VTPYLTEAQITSGTLSYALALGRPVVSTPYWHAVEALSGGVGIVCPFQDAAAFTAAIA
                     GLLASEGRRAEMSLRAYEAARPSRWSAVAEAYIQRAEADVQTRIDEKTKPGTVDAALP
                     AQPPLAALARMSDDCGVAQHGALRLPDRRHGYCVDDNARALSLMARLSHLEPPPPGRE
                     AMTRAYAAFVNHAWLPEGRFRNFMAYDRRWLDEGGSDDACARTFESLIDVARSNLPAD
                     LRQWACDLGGRVLAHVARWPSLRARAVLTRTLIRGCGVVGDEQAVRGLTRDFCADLHA
                     RLRRFTRPGHVWFEPELSYDNARIPEALILGGRFLSEGDYVRDGLAALRWLMQVQTHA
                     PTGVFMPAPTSRFDVQGQDHPLYDQQPIEALASVEACLSAAQVSGDDGWKAEARRALD
                     WFTGRNTQHLSLIDEDGGCRDGLTPNGRNENQGAESLLAWPLSWLAMRLNA"
BASE COUNT          761 a         1623 c         1637 g          718 t
ORIGIN      
        1 cgcgacgtca tgacgatcgc gcgcgacaac ggccttctga tcgcgggcgg ttcggacaac
       61 tgcatccgcc ttttgccgcc gctgaacctg accgaggacg aggcgcgcga agccatcggc
      121 ctgttggaca agaccttcgc cgaggcgcgc gaaaagctga aggagagcgc gtgatgaagc
      181 actttctcga catctgcgac ctgaccccgg cggacctgcg cgccatcctc gacgacgcgc
      241 acgcgcgcaa ggccgcgcgc cggggctggc ccaagggccg cgtcgacgcc gacgcgccgg
      301 cgcaggaccg cgtgctggcc atgatcttcc agaagaattc gacgcgcacg cgcttctcgt
      361 tcgacgcggc gatccggcaa ctgggcggcg acgcgatcat ctcgaccgcc tcggacatgc
      421 agcttgggcg cggcgagacg atcgaggaca cggcgcgcgt gctgtcgcgc atggtcgacg
      481 cgatcatgat ccgcgccaac gaacacgacg acgtggtgcg gctggcgcgc gcgtccaccg
      541 tgccggtgat caacggcctg accaaccttt cgcatccgtg ccagatcatc gccgacctcc
      601 agaccatcga ggagcatcgc ggaaacatcg cgggcaagaa gctggcctgg atcggcgacg
      661 gcaacaatat gtgcacaagc ttcatccacg cggcggcgcg cttcggcttc gagctggcca
      721 tcgcgtgccc cgacgcctac cggccgaacc cgcaggcgct ggtcttcgcc tcggaatcgc
      781 gcgccagggt gacggtgagc aacgatccgg ccgccgcggc cagggacgcc gacgtggtgc
      841 tggccgacac ctgggtgtcg atgggcgacc tcgaccacga cgaacggctg gcggcgttcc
      901 agccctatca ggtcaatgcc ggtctgatga agctggccaa gcccgacgcg ctgttcctgc
      961 actgcctgcc cgcgcaccgc ggcgaggagg tgacggacga cgtgatcgac ggcccgcaat
     1021 cggtggtctg ggacgaagcg gaaaaccgca tccacgccca gaaggccgtg atcgcatggt
     1081 gtttcagcta gggcagggtt gcccctccca ccattgctgc gcaatggtcc cccctccccg
     1141 taaacggggc gggcgaatac ggcgacgacc cgcctccccc gttcacgggg gaggcggcat
     1201 tggcgaagcc aatgacggag gggggcctca aacccccaga tgcgccagca tgtccttcac
     1261 atggatcgag gcgaaatgga tgctggaatc cgccacgccg tagggcatga agatccagtc
     1321 cccgacgcgc atcgcgccgc aggtgtagat gacgttcggc acatagccct cgcggttatc
     1381 ctccgacggc gacaggagcg gcgtcttcga ccgcgcgatc agcttgcgcg gatcgtcctt
     1441 gtccagcagc gccgcgccca gcgaatagcg ccgcaccgcg ccgacgccgt gggtcagcaa
     1501 aagccagccc tcgtccagtt cgatgggcga gccgcaattg cccatctgga tcagctccca
     1561 cggcgcctcc ggccccatga tgcgctcgcc gttgttccac gcatagcggt cgtcgctttc
     1621 gagatagaac aggctttcgt gatccagccg cgcgatggcg gcgtagcgtc cgttcatgcg
     1681 gcgcgggaaa aaggccagcc ccttgtgctg cgaggccgcg ccggtgaagg gcgtcagccg
     1741 gaaggcgcgg aagtcctgcg tctcaaggca ttcgcaggcg gtttcgcgcc ctgaataggc
     1801 ggtgtaggtg ccgatgtagg cgcggctgcc gtcctcgtcc tcgaaccagg tcaggcgcag
     1861 atcctccagc ccgttcgact gggcgcgcgt caccgggaac agcacggttt ccgagatcgg
     1921 gcacgcctga tggccgcgca cctcgaccgc gccgtccagc ggcgcgttga ggtcgggctt
     1981 ggcggcgacg gcgaaatcgg cttcgggcca aagctccatg gttccgtcgg gatagaagac
     2041 gccctcgcgg aacgagatgg tcgaggtgtg cccctcgccg accgtgcgcg ccgacatgac
     2101 gaagttgacg ccgccgttgg cgttgctctg gtcgggatgc ggcacgaccg acgggttcat
     2161 gatggcggcg gcctgatagc tgtattcatg gcagaaatag gcgccgatca gggcctgatg
     2221 gtcctcgtcg agattttcca tgtcgccctg catctgctgg atctggcgga agcggtcgag
     2281 gaagatgccg cgcgtctgcc agtgccgccc ggtgaagtcg cggttgacca cctccagctc
     2341 ggcgcggcac tgctcgctcg acagggcggt cagggccgcg cagatgcgcg tgacccgcga
     2401 catggcgccc atggccgaat cgcgcggcat gatcgagatc gagaacggca ggatgaccac
     2461 gcgcgccggg tcggcgcgca gggtggtcgg gctcatggtc atctgtgtca taatatccgc
     2521 cggtaagtgt gtgggttacg cgttcagccg catcgccagc cagctcagcg gccaggccag
     2581 cagcgattcc gcgccctggt tttcgttgcg tccgttcggc gtcagcccgt cgcggcagcc
     2641 gccgtcttcg tcgatcagcg acaggtgctg cgtgttgcgt ccggtgaacc agtccagcgc
     2701 ccgccgcgcc tcggccttcc agccgtcgtc gccgctgacc tgcgccgcgc tcaggcaggc
     2761 ctcgacgctg gccagcgcct cgatcggctg ctggtcgtac agcggatggt cctgcccctg
     2821 cacgtcgaag cgcgaggtcg gcgcgggcat gaacacgccc gtcggcgcgt gggtctgaac
     2881 ctgcatcagc cagcgcagcg ccgccagccc gtcgcgcacg tagtcgccct cggacaggaa
     2941 gcggccgccc aggatcagcg cttcgggaat gcgcgcattg tcgtagctca gctccggctc
     3001 gaaccagaca tggccgggcc gcgtgaagcg ccgcagccgc gcgtgcaggt cggcgcagaa
     3061 atcgcgcgtc agcccccgca cggcctgttc gtccccgacc acgccgcagc cgcggatcag
     3121 cgtccgcgtc aggacggcgc gcgcgcgcag ggacggccag cgcgcgacat gcgccagaac
     3181 gcgtccgccc agatcgcacg cccattgccg cagatcggcg ggcaggttgg agcgcgcgac
     3241 gtcgatcagg ctttcaaagg tccgcgcgca ggcgtcgtcc gagccgcctt cgtccagcca
     3301 gcgccggtcg taggccatga agttgcggaa gcgcccttcg ggcagccagg cgtgattgac
     3361 gaaggccgca taggcgcgcg tcatcgcctc gcgccccggc ggcggcggct ccagatggct
     3421 gagccgcgcc atcagcgaca gcgcgcgcgc attgtcgtcg acgcaatagc cgtggcggcg
     3481 gtcgggcagg cgcaaggctc cgtgctgcgc gacgccgcaa tcgtcgctca tgcgcgccag
     3541 cgccgcgagc ggcggctgcg cgggcagggc cgcgtccacg gttccgggtt tcgtcttttc
     3601 gtcgatacgg gtctgcacgt ccgcctccgc gcgctggatg taagcctcgg ccaccgccga
     3661 ccagcgcgag ggccgcgccg cctcgtaggc gcgcaggctc atctccgcgc gccggccttc
     3721 cgaggccagc agcccggcga tggcggcggt gaaggcggcg gcgtcctgga acggacagac
     3781 gatcccgacc ccgccgctca gcgcctcgac cgcgtgccag tagggcgtcg acaccaccgg
     3841 ccgccccagg gccagcgcgt aggacagcgt gccggaggtg atctgcgcct cggtcaggta
     3901 cggggtgaca tagatgtcgg tggcctgaag gatgtcgatc agttcgtcgt cgccgatgaa
     3961 gcggtcgatg aagcggacat ggtcctcgac gccgagatcg cgcgccatcg ccttcaggcg
     4021 ctcgcgatag gcttcgccct catgccggat caggtgcgga tgggtggcgc cgatgacgag
     4081 atagacgagg tccggcgcct gccgcgccag ttcgggcagg gcggcgatca ccgtctcgac
     4141 gcccttgttc ggcgacaaaa ggccgaaggt ggtcagggtg cggcgcccgc tcaggcccat
     4201 ccgctccttg aaggcggcgg tgtcgcgcaa gggccgcgac ggcgcgccgt gcggcacgac
     4261 ggcgatctgg ccgtccggga cgccatagac ggttttcaga atttccgcac ccttcctcgc
     4321 catggtcacc agcgtcgtgg acaggcggat cagcccctcc atcacccggc gctgctccgg
     4381 cgtcggacgc gccagcaccg tgtgcagggt ggtgacgatc ggcatgtcga ccgcttcgag
     4441 caggtccagc agataggcgc cggacgggcc gccgtagatg ccgaattcgt gctgcacgaa
     4501 caaagcctcg gtcccgtcct cgcgcaacga gcgcgccagt tgcgcatagg ccgcctgatc
     4561 gtcctgaaag atcgcgtcgg tgaccgcgtc gggataggcg taaccggcgt cgggcgcatc
     4621 gttcatggcg atcacgcgcc acgccgcgtc cggcagcgcc ttcgacaggc aggcgtacat
     4681 gtcgcgcgta aaggtcgcca gcccgcactg acgcggcggg aaattgccga tgatggcgg
//
LOCUS       DASVLL010000467        11171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_191017,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000467 DASVLL010000000
VERSION     DASVLL010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191017"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /gene="nirB"
                     /locus_tag="VF402_07725"
     CDS             complement(<1..1863)
                     /gene="nirB"
                     /locus_tag="VF402_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEX7800224.1"
                     /translation="MTQHKERLIVIGAGMAGGRIVEEILKRDETRFDISIIGAESLPT
                     YDRIQLSPVLAGEKRFDQIVTHSQDWYDSNGVKTHFGYWVQSIDRVRKEVMLHDGQRI
                     GYDRLILATGSDPIRLPLPGADLSGVVAFRDLHDVDAMQAAAEQGGEAVVIGGGLLGL
                     EAAYGLAKRGMKATVIHLVDVLMERQLDEAAGRLLEKALIERGVASILNAQSEAILGE
                     THVEGLKLKDGRVIPCSLLVMAVGIRPHVEPARSAGLTVERGIVVDDQMRTLDPDIFA
                     VGECVQHRGQCYGLVAPIWDMCRTLADVLTEKNPDAAFVGTSLATRLKVSGVDLYSAG
                     QFGGGEGCEDIVFRDPGRGVYKRIVIKDDKLVGTVLFGEAADGGWYFDLMKKGEDIAP
                     IRDTLIFGQAVTSALAGADPSDAVAAWPDDTEVCGCNGVSKGQVVASICAGNDTLDKV
                     RGHCKASASCGSCTGIVESLLKVTLGDAFKAQSGPKPVCKCTAHGHAHVRKAIVEQGL
                     KTIPDVMQALKWATPDGCSSCRPALNYYLLCAWPREYKDDPRSCFVNERNHANIQKDG
                     TYSVVPRMWGGLTSAKELRAIADVVDKYDLPMVKVTGGQRLDIFGVKKEQLPQVW"
     gene            complement(2089..3303)
                     /locus_tag="VF402_07730"
     CDS             complement(2089..3303)
                     /locus_tag="VF402_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX7800225.1"
                     /translation="MISKDFLKSGHTPTLFSAFLYFDMSFMVWVLLGALGVQISKDLG
                     LSPAEKGLMVAVPILAGAALRIVNGFLVDMIGPKKTGALAQAVVIIGLLAAFFLHIDS
                     YGAMLAMGGVLGMAGASFAVALPLASRWYPPKYQGVALGIAGAGNSGTVLASLFAPTL
                     AKTFGWQSVFGMAALPLIAVFVLYMLLAKDAPDAPPRKTLSQYANILRQADAWWFMLF
                     YGVTFGGFVGLSSSLNIYFNSEYGLSPVTAGFFTAACVFIGSLVRPIGGAVADRVGGV
                     RSLSVMYVIAAAALVALSFGMPSVWGALIVLLVGMGALGMGNGAVFQLVPQRFRSEIG
                     IMTGLVGAFGGVGGFYLAASLGLSKQITGSYQIGLVGFALLALIALCGLTLVKKTWRT
                     TWHVYGAADAKI"
     gene            complement(3318..4436)
                     /locus_tag="VF402_07735"
     CDS             complement(3318..4436)
                     /locus_tag="VF402_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEX7800226.1"
                     /translation="MFADITPLLDSNLRYAHVEQAGFANDADALTWRNRFGVQSRQGP
                     FSGLIEFENVTALVQDYNSGLNGKTAYPQESDPQVTELNRAQVTWTPDAHSALTVGRQ
                     RIILDDARFVGNVGWRQDEQTFDAVRYDLKSGGFALTAAYLTKINRVLGEEKDWDSDS
                     YILNAGYALHGVKLTAFDYALDFANAAASSTLTLGARATGGFNLASVKVNLTGQYARQ
                     SDYRNNPAEFELQESMLEVAATYRIASFKINYESLGGDGKVGFVTPLGTTHAFEGFAD
                     AFSGTGGNKTTANGLDDLNCAATLSLPHKSALTLIYHDLSTARLGVGLGHEWDAVATV
                     GLTKHLSLLAKYADYDASASPLAPASRRKTWLALSYKL"
     gene            complement(4788..5933)
                     /locus_tag="VF402_07740"
     CDS             complement(4788..5933)
                     /locus_tag="VF402_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7800227.1"
                     /translation="MNLTLAYNPLNDAALLLVAEALGFYDSEGLSVTLSREANWSTVR
                     DKLAYGMIDGAHILAPMPFAAALGQGPSMGRIIAPMALGAGGNLVLVSAGLKRALEED
                     GAAPKTLRGSARLLAREVAARRYAGAGRLVFAVPFTFSPHHFVLRHWVAAAGLDPERQ
                     VQWVVAPPSRMADMVRDGVIDGFCSGSPWPQTAELNGDGEVLFSDPDFWTLKPEKVLG
                     VRAGWAEANPDGVIALVRALMRAAAWASDPAHRGDLIRILARKAYVGAPAEAIAAALG
                     DQGAGLTLDPQTATFPWISHAKWFLGQMVRWGWLAPDHDFDALARRIYRPDLWREAAG
                     GLGLALPLADEKDEGGQAAGWSLPAKPQAIDMPPNALFDGGVFRCGS"
     gene            complement(5930..6520)
                     /locus_tag="VF402_07745"
     CDS             complement(5930..6520)
                     /locus_tag="VF402_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX7800228.1"
                     /translation="MKVLVIDPDETRATLVADGIRAEYGGPDTLIRQEARFELRMLRD
                     LDPDMVIIACESPDRDMLEALQLANDAAPRPVVMFVDRSDANATAKALEAGVVGYIVD
                     GFNPARIASILSVAASRFEVMQGMRRDLDKARADLAARKTIDRAKGVLMASRNLSEDE
                     AYKLLRKHAMDTGRPMQAVAADILAVSDLLKPGSAS"
     gene            6796..8826
                     /locus_tag="VF402_07750"
     CDS             6796..8826
                     /locus_tag="VF402_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX7800229.1"
                     /translation="MRRAHGLRDGARHLGASLAAAWRGTSLSVRLSLGALIVLLLAVV
                     VFLAQPNWNWARGAVASLISARLHRPVGIAGNLRVRLFSFTPSATVTGLRVGQPTGFV
                     KDAPKDDLASVAALSASVQLMPLFVGHVVLPRVEADGPVVTLYQDGAGHANWDFSNGA
                     AKGQPLRLPLIRNFVIHDGHIRMTSLSRRMRFAGTVEAHEKADAGGQAFGLTGDGGLN
                     GKAFTLKATGGPLLNVRSGTPYPFDLIVTAGGTRIAARGRVLHPFDLGRLQGAVTVSG
                     GNLADLYYLTGLTLPDTPAYRLSAQVRRDDRAYDIAGIDGRVGHSDLEGALKVVLARD
                     GRPRLSGDLTSRRLDFKDLGALFGATSANAPQGAAVAVNPEPTAAARRLLPDVPLDVA
                     RVRGMDAAVHYRARSVNASPNLPLRAVDLGVKLDQGLLRLDPIAFDFPPGRLKGTARI
                     DARTDAQTNSVDFTVSDIAVQQFTPKFQNAEPVEGRISARLRATGAGHSVHEAAASAN
                     GRFALVMPGGTVRQSLAELMGVDATKGLFMLLAKDPHQTDVRCAVADFAVRDGVMTAQ
                     RIVFDTGVVVVNGSGRVDLRDESLKLVFKGKAKKFRLVRLNAPIVIGGHLTRPVFGVD
                     PGPALAQGGAGAALGAVSAGLAVLPFLNLSGAPDADCAALMAEAQQHGAPKK"
     gene            8850..10226
                     /locus_tag="VF402_07755"
     CDS             8850..10226
                     /locus_tag="VF402_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7800230.1"
                     /translation="MADASDLKQHFTDAPGLSAAMADLLGDVTDLRVLEPSVGQGALL
                     TGLRGVPAHIDAVDIDPRTLAQARARLAHLPLRAHPGDFLALDLPRDYDAVIANPPFG
                     LYFTPQARKTLKQRFPGLYVRESYGLFFVQAVRRLRPGGRYVFLLPDSFLTSRHHAPL
                     RRFIADEAAVTEIVRFPSRRFASVNFGYGHMCLIAGRRAALTARDRIGWRDAFGDAPL
                     LEQARVRLTGADLIGALDDGWRPARDVLPDGWTRLGAVAECRTGIYTGDNPRFLGYDP
                     RRVTKRLNGHPIDWDRDVGAGYVPLVRGGHRAFDTAPDWAIRWDAAALAHYRNDKKAR
                     FQNSGWYFRAGLAVPMVTSRRLSASLMQDAVFDQGVVGVFPRDPAQRAALLLYLNSSA
                     ATRLRNERLGGSANNSANYLKRLPVPAFDAAAAARAQGVVARAIRDGDLPQAVCDAFV
                     GDASGGAA"
     gene            complement(10209..>11171)
                     /locus_tag="VF402_07760"
     CDS             complement(10209..>11171)
                     /locus_tag="VF402_07760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7800231.1"
                     /translation="YGGPSTSFRVYLKNYDPAYADRADDESPQYLGVETNTTPGRWQR
                     AEFTPDDFGVAEWWMRKRRLPPRYGHPQFKDITSMIVETGSEAPLGRHDFQVREIVVR
                     RALLSEAQWYSLLLGVWIVMIVGYLGYRVVNLRRALRERRILDALALREAQEAANHDP
                     LTGLLNRRGMGERFDGLTAARGRTAGLAAILLDVDHFKALNDAHGHACGDRVLSDLAG
                     VIRRHVRAADLAARWGGEEFLVVCAGVNRRGAQRVAEKLRACIESHDFGVGAPVTASF
                     GVHWSSAGEPDLAQRVALADKALYAAKAAGRNCCRVYPLLSRAA"
BASE COUNT         1777 a         3797 c         3792 g         1805 t
ORIGIN      
        1 ccagacctgc ggcaactgct cttttttcac gccgaaaatg tcgagccttt ggccgcccgt
       61 gaccttgacc atcggcaggt cgtacttatc gaccacatcg gcgatggcgc gcagctcctt
      121 cgcgctggtc aggccgcccc acatgcgcgg cacaacgctg taggtgccgt ccttctggat
      181 attggcgtgg ttgcgctcat tgacgaagca cgaacgcgga tcgtccttgt attcgcgcgg
      241 ccaggcgcac aacaggtagt agttcagcgc cggacggcac gacgaacagc cgtcgggcgt
      301 cgcccatttg agcgcctgca tcacgtcggg gatggttttc aggccctgct cgacgatggc
      361 tttacgcaca tgcgcatggc cgtgcgcggt gcatttgcac accggcttcg gcccgctttg
      421 cgctttaaac gcgtcgccca gcgtgacctt cagcaggctc tcgacaatgc cggtgcagga
      481 gccgcaactg gcggacgcct tgcaatggcc gcgcaccttg tcgagcgtgt cgtttccggc
      541 gcagatcgac gcgaccacct ggcccttgga tacgccgttg cagccgcaca cctcggtgtc
      601 gtcaggccat gccgcaacgg cgtcagaagg gtccgcgccg gccagggcgc tggtcaccgc
      661 ctgaccgaag atcagcgtat cgcggatggg ggcgatgtcc tcgcccttct tcatcaggtc
      721 gaaataccag ccgccgtccg ccgcttcgcc gaacagcacc gtgccgacca gcttgtcgtc
      781 cttgatgacg atgcgcttat agacgccgcg gccgggatcg cggaacacga tgtcctcgca
      841 gccctcgccg ccgccgaact gaccggcgga atagagatcg acgcccgaaa ccttcagccg
      901 cgtcgccagc gaagtcccga cgaaggccgc gtccgggttc ttttcggtca gcacgtcggc
      961 cagggtccgg cacatgtccc agatcggcgc gacgaggccg tagcactggc cgcgatgctg
     1021 cacacattcg ccgaccgcga agatgtcggg atcaagggtg cgcatctggt cgtcgaccac
     1081 aatgccgcgc tcgaccgtca atccggcgga cctggccggt tcgacgtgcg gccggatgcc
     1141 gacggccatg accagcagcg aacacggaat cacgcgcccg tctttcagct tcaggccctc
     1201 gacatgggtt tcgcccagaa tagcctcgga ctgggcgttc aggatcgagg cgacgccgcg
     1261 ttcgatgagc gctttttcca gcagccgccc ggcggcttcg tccagttgcc gctccatcag
     1321 cacgtcgacc agatggatga cggtggcctt catgccgcgt ttcgccagcc cgtaggcggc
     1381 ttcgagaccc agcagcccgc cgccgatgac gacggcttcg ccgccctgtt ccgccgccgc
     1441 ctgcatcgcg tcgacatcgt gcaggtcgcg gaaggcgacc acgccggaaa ggtcggcgcc
     1501 gggcaggggc aggcggatcg ggtcggagcc ggtggcgagg atcagcctgt catagccgat
     1561 gcgctggccg tcatgcagca tgacctcctt gcgcacgcgg tcgatggatt gcacccaata
     1621 gccgaagtgg gtcttgacgc cgttgctgtc gtaccagtcc tgcgagtggg tgacgatctg
     1681 gtcgaagcgc ttttccccgg ccagcaccgg cgaaagctgg atgcggtcgt aggtgggcag
     1741 gctctcggcg ccgatgatcg agatgtcgaa gcgcgtctcg tctcgcttga ggatttcctc
     1801 gacgatgcgg cccccggcca tgccggcgcc gatgacgata agccgctctt tgtgctgggt
     1861 catgcgaacc tcttgagcaa aaaaaagagc cccgaaacgc gccttccaga tggaaagccg
     1921 cttcgggact ccgttgtcct tgatgatgcg ttgcaaaaac cgccgttggt tcctgcgccg
     1981 cgaatgtcgt tagccggtct ttggctagga cttacagcgt atgacagttc tgcggattgt
     2041 caacgcggaa aaagcgcggc gcacaaattt tgtgcgccgc gataagattc aaatcttggc
     2101 gtcggccgcg ccatagacat gccaggtcgt gcgccaggtc tttttcacca gggtcaggcc
     2161 gcaaagcgcg atcagggcca gaagggcgaa gccgacgagg ccgatctgat agctgccggt
     2221 gatctgcttg gacaggccca gcgaggcggc gagatagaag ccgccgacgc cgccgaaggc
     2281 gccgacgagg ccggtcatga tgccgatctc gctgcggaag cgttgcggca ccaattgaaa
     2341 gaccgcgccg ttgcccatgc ccagcgcgcc catgccgacc agcagcacga tgagcgcgcc
     2401 ccagacgctg ggcatgccga agctgagcgc gaccagggcg gcggcggcga tcacgtacat
     2461 caccgacagg ctgcgcacgc cgccgacccg gtctgcgacc gcgccaccga tcggccgcac
     2521 cagcgagccg atgaacacgc aggcggcggt gaagaagccc gccgtgaccg gcgacaggcc
     2581 gtattcgctg ttgaaataga tgttgagcga cgaggacagg ccgacgaagc cgccgaaggt
     2641 gacgccgtag aacagcatga accaccacgc gtccgcctga cgcaggatgt tggcgtattg
     2701 cgaaagcgtc ttgcgcggcg gcgcgtccgg cgcgtctttc gccagcagca tgtagaggac
     2761 gaagaccgcg atcagcggca gggccgccat gccgaacacc gactgccagc cgaacgtctt
     2821 ggccagggtc ggcgcgaaca gcgaggccag cacggtgccg gaattgcccg ccccggcgat
     2881 gccgagcgcc acgccctgat atttcggcgg ataccagcgc gaggccagcg gcagggcgac
     2941 ggcgaacgag gcgcccgcca tgcccagcac gccgcccatg gccagcatgg cgccatagct
     3001 gtcgatatgc aggaagaagg cggccagcag gccgatgatg accacggcct gcgccagcgc
     3061 gccggtcttt ttcggcccga tcatgtcgac cagaaagcca ttgacgatgc gcagggccgc
     3121 gcccgccaga atcggcacgg cgaccatcag gcccttctcg gcgggactga ggcccagatc
     3181 cttggagatc tgcacgccga gcgcgcccag cagcacccag accatgaagc tcatgtcgaa
     3241 gtacaagaag gcggagaaca gggtgggcgt atgcccgctt ttcaggaaat ccttggagat
     3301 cataacgtgc gatcctctta aagtttatag ctgagcgcca gccatgtctt gcggcgcgag
     3361 gcgggggcga gcggggaggc ggaggcgtcg taatcggcgt acttggccag cagcgacaga
     3421 tgtttggtca ggccgaccgt ggcgaccgcg tcccattcat ggccgaggcc gacgccgagc
     3481 cgcgccgtcg aaaggtcgtg ataaatcaac gtcaaggccg acttgtgcgg caggctgagc
     3541 gtggccgcac agttcaggtc gtcgaggccg ttcgcggtcg tcttgttgcc gcccgtgccg
     3601 gaaaaggcgt cggcgaagcc ttcgaaggcg tgcgtcgtgc cgagcggcgt gacgaagccg
     3661 accttgccgt cgccgccgag actttcataa ttgatcttga agctggcgat ccggtaggtc
     3721 gcggcgacct ccagcatgct ttcctgaagc tcgaactcag ccggattgtt gcggtagtcg
     3781 ctctggcgcg cgtactggcc ggtcagattg accttgacgc tggcgaggtt gaagccgccg
     3841 gtggcgcgcg cgcccagcgt cagggtcgag gacgccgccg cgttggcgaa gtcgagcgca
     3901 tagtcgaagg cggtcagctt cacgccgtgc agggcgtaac cggcgttcag gatatagctg
     3961 tcgctatccc agtccttttc ctcgcccagc acgcggttga tcttcgtcag ataagccgcc
     4021 gtcagggcga aaccgccgct tttcagatcg tagcgcacgg cgtcgaaggt ctgttcgtcc
     4081 tgccgccagc cgacattgcc gacgaagcgc gcgtcgtcga ggatgatgcg ctggcgcccg
     4141 acggtcaggg cggaatgcgc gtccggcgtc cacgtcacct gcgcgcggtt cagttcggtg
     4201 acctgcgggt cggattcctg tggataggcg gtcttgccgt tcaggccgga attgtagtcc
     4261 tgcacaagcg ccgtgacgtt ctcgaattcg atcaggccgg aaaacgggcc ttgccggctc
     4321 tgaacgccga agcggttgcg ccaggtcagg gcgtcggcgt cgttggcgaa accggcctgc
     4381 tcgacatgag cgtagcgcag gttggaatcg aggaggggag tgatgtccgc aaacacgggg
     4441 gagcgtcctt ttcgggcata aaaaaacgcc tcaagccgga ccggccccgt ctgggggttc
     4501 agtctgtcct gagacgtcgt tgtcggatgt gacggccgcc ctgcgccgtc cttggcgcga
     4561 aaaagcggct caatcggcga tcttcgttga ccgcatgacc ctgagactgc gggcgaacgc
     4621 gccgggcgtc aatgcgggtt tgagttatgt tgctctgcac aattttcgtg tggcgaaaac
     4681 gtcacaaaag ggcggggcag ggggcggaaa tggcggtggg gcgggttagt tgggagatgg
     4741 ggcattatgt ttcttttgaa actttcttgg tcgcgggaag aaacccctca agacccgcag
     4801 cgaaacaccc ccccgtcgaa caaagcgttc ggcggcatgt cgatggcctg cggcttcgcg
     4861 ggcagcgacc agcccgccgc ctgcccgccc tcgtccttct catcggccag cggcagggcc
     4921 aggcccagcc cgcccgccgc ctcgcgccac agatcggggc ggtagatcct tcgcgccagc
     4981 gcgtcgaaat cgtggtccgg cgccagccag ccccagcgca ccatctggcc caggaaccat
     5041 ttggcgtgac tgatccacgg gaaggtcgcg gtctgcggat cgagcgtcag cccggcgccc
     5101 tgatcgccca gcgccgccgc gatcgcttcg gcgggcgcgc cgacataggc cttgcgcgcc
     5161 agaatgcgga tcaggtcgcc gcgatgcgcc ggatcgctgg cccacgccgc cgcgcgcatc
     5221 agggcgcgca ccagcgcgat cacgccgtcg gggttcgcct cggcccagcc cgcgcgcacg
     5281 cccagcacct tttccggctt cagcgtccag aagtccgggt cggaaaacag cacctcgccg
     5341 tcgccgttca gctcagccgt ctgcggccat ggcgagccgg agcagaagcc gtcgatcacc
     5401 ccgtcgcgca ccatgtcggc catgcgcgac ggcggcgcca ccacccattg aacctgccgt
     5461 tccgggtcga gccccgccgc cgcgacccag tggcgcagca cgaaatggtg cggcgaaaag
     5521 gtgaagggca cggcaaaaac cagccttccg gcgccggcat agcgccgcgc cgcgacctcg
     5581 cgcgccagca gccgcgcgct gccgcgcaag gttttcggcg ccgcgccgtc ctcctccagc
     5641 gcgcgtttca gccccgccga caccagcacc aaattaccgc ccgcccccag cgccatcggc
     5701 gcgatgatcc gtcccatcga cggcccttgc cccagcgccg ccgcgaacgg catgggcgcc
     5761 aggatgtgcg cgccgtcgat catgccgtag gccagcttgt cgcgcacggt ggaccagttg
     5821 gcttcgcgcg acagggtgac gctcaggccc tcgctgtcgt aaaaaccgag cgcctcggcg
     5881 accagcagca gcgccgcgtc gttgagcgga ttgtaggcga gggtcaggtt catgacgcgc
     5941 ttcccggttt cagaaggtcg ctgacggcca gaatgtcggc ggccacggcc tgcatgggcc
     6001 gccccgtgtc catagcgtgc ttgcgcagga gtttataggc ttcgtcctcg ctgagattac
     6061 gcgacgccat cagcacgccc ttggcgcggt cgatggtctt gcgcgcggcg aggtcggcgc
     6121 gcgccttgtc gaggtcgcgg cgcatcccct gcatgacctc gaagcgcgag gcggcgacgc
     6181 tgaggatcga ggcgatgcgc gccgggttga agccgtcgac gatatagccg accaccccgg
     6241 cctccagcgc cttggccgtg gcgttggcgt cggagcggtc gacgaacatc accaccggac
     6301 gcggcgccgc gtcgttggcc agttgcagcg cctccagcat gtcgcggtcc gggctttcgc
     6361 aggcgatgat caccatgtcg gggtcgaggt cgcgcagcat gcgcagctcg aagcgcgcct
     6421 cctgacggat cagcgtgtcc ggcccgccgt attcggcgcg gatgccgtcc gccaccaggg
     6481 tcgcgcgcgt ctcgtcggga tcgatgacca gaaccttcat atagtccgtc ggcagtccat
     6541 aaaaaaagcc gccccaaccg gccttcggcg tcgtccgaag cggctgaggg cggcgttgcc
     6601 ccggccggtc gtccgcattc gcgtcgcccg ccgtctctta gcatgtgcaa aaaatttatc
     6661 cttggcaaga gacattccca cgcgcgcctt acggatcgat ggcggcccga cgctgcgtcc
     6721 ttatctttcc ctgtggataa tgcgcgcatg actcatgcga cgcggttcga ttcagaggcg
     6781 atacgcgcat ggtccgtgcg ccgggcgcac ggcctgcgcg acggcgcgcg ccatctgggc
     6841 gccagtcttg cggcggcgtg gcgcggaacc tcgctgagcg tgcggctgag cctcggcgcg
     6901 ctgatcgtgc tgctgctggc ggtcgtcgtc tttctggcgc agccgaactg gaactgggcg
     6961 cgcggcgcgg tcgcctcgct gatctcggcg cggctgcatc gcccggtcgg catcgccgga
     7021 aacctgcgcg tgcgcctgtt cagcttcacg ccgtcggcga cggtgaccgg cttgcgggtg
     7081 ggccagccca cgggcttcgt gaaggacgcg ccgaaggacg atctggcctc tgtcgccgcc
     7141 ctgagcgcca gcgtgcagct catgccgctg ttcgtcggcc atgtcgtgct gccgcgcgtc
     7201 gaggcggacg ggccggtcgt gacgctttat caggacggcg ccggtcacgc caactgggac
     7261 ttcagcaacg gcgcggcgaa gggccagccg ctgcgcctgc cgctgatccg caacttcgtc
     7321 atccacgacg gccatatccg catgaccagc ctgtcgcgca ggatgcgctt cgccggaacc
     7381 gtcgaggcgc acgaaaaggc cgacgcgggc gggcaggcgt tcggcctgac cggcgacggc
     7441 gggctgaacg gcaaggcgtt cacgctgaag gccacgggcg gtcccttgct caatgtgcgc
     7501 agcgggacgc cctatccgtt cgacctgatc gtcacggcgg gcggcacgcg catcgccgcg
     7561 cgcggacggg tgctgcatcc gttcgatctg ggccggttgc agggcgcggt gacggtgagc
     7621 ggcggcaatc tggccgacct ctactacctg accggcctga ccctgcccga cacgcccgcc
     7681 taccggctga gcgcgcaggt gcgccgcgac gaccgcgcct atgacatcgc gggcatagac
     7741 gggcgcgtcg gccacagcga cctcgaaggc gcgctcaagg tcgtgctggc gcgggacgga
     7801 cgcccgcgcc tgagcggcga cctgacctcg cggcggctgg atttcaagga tctgggcgcg
     7861 ctgttcggcg ccacctcggc caatgcgccg cagggcgcgg cggtggcggt caatcccgaa
     7921 ccgacggcgg cggcgcgccg cctcttgccc gacgttccgc tcgacgtggc gcgggtgcgc
     7981 ggcatggacg ccgctgtgca ttaccgcgcg cggagcgtca acgcctcgcc gaacctgccg
     8041 ctgcgcgcgg tcgatctcgg cgtcaagctg gatcaaggcc tgctgcgcct cgatccgatc
     8101 gccttcgact ttccgccagg ccgcctgaaa gggaccgcgc ggatcgacgc gcgcaccgac
     8161 gcccagacca acagcgtcga tttcaccgtc agcgacatcg ccgtgcagca gttcacgccg
     8221 aaattccaga acgccgaacc cgtcgagggc cggatcagcg cccgcctgcg cgcgaccggc
     8281 gcaggccatt ccgtccacga agccgccgcc agcgccaacg ggcgcttcgc cctggtcatg
     8341 ccgggcggga cggtgcggca gagcctggcc gaactgatgg gcgtggacgc gaccaagggc
     8401 ctgttcatgc tgctggccaa agacccgcac cagaccgacg tgcgctgcgc ggtcgccgat
     8461 ttcgccgtgc gcgacggcgt gatgacggcg cagcgcatcg tgttcgacac cggcgtggtg
     8521 gttgtcaacg gttcgggccg cgtcgacctt cgggacgaga gcctgaaact ggtgttcaag
     8581 ggcaaggcca agaagttccg cctcgtgcgg ctcaacgcgc ccatcgtgat cggcggccac
     8641 ctgacccggc ccgttttcgg cgtcgatccc ggtccggccc tggcgcaggg cggcgccggc
     8701 gcggcgctcg gcgcggtgtc ggcggggctg gcggtgctgc cgttcctgaa cctgagcggc
     8761 gcgcccgacg ccgactgcgc cgcgctgatg gccgaggcgc agcagcacgg cgcgccgaaa
     8821 aaatagcgac tcatggttta agtcggcgca tggccgacgc ttccgacctc aaacagcatt
     8881 tcaccgacgc ccccggcctc agcgcggcga tggcggacct gctcggcgac gtgacggatt
     8941 tgcgcgtgct ggagccgtcg gtggggcagg gggccttgct gaccggcctg cgcggcgttc
     9001 cggcgcatat cgacgcggtc gacatcgatc cgcggacgct ggcgcaggcg cgggcgcggc
     9061 tggcgcatct gccgctgcgc gcgcatccgg gcgactttct cgcgctcgac ctgccgcggg
     9121 attacgacgc ggtgatcgcc aacccgcctt tcggcctcta tttcacgccg caggcgcgca
     9181 aaacgctgaa acagcgcttt ccgggcctct acgtgcgcga atcctacggc ctgttcttcg
     9241 tgcaggcggt gcggcggctg cggcccggcg ggcgttacgt cttcctgctg ccggacagct
     9301 ttttgaccag ccgccatcac gcgccgctgc gccgcttcat cgcggacgag gccgccgtga
     9361 ccgagatcgt gcgctttccg tcgcgccgct tcgccagcgt caatttcggc tacggccata
     9421 tgtgcctgat cgccgggcgg cgcgccgcgc tgacggcgcg ggaccgcatc ggctggcgcg
     9481 acgccttcgg cgatgcgccg ctgctggagc aggcgcgcgt gcgcctgacc ggggccgatc
     9541 tgatcggcgc gctcgacgac ggctggcgcc cggcgcgcga cgttctaccc gacggctgga
     9601 cgcggctggg cgcggtcgcc gaatgccgga ccggcatcta taccggcgat aatccgcgct
     9661 ttctcggcta tgatccgagg cgcgtgacga agcgcctgaa cggtcatccg atcgactggg
     9721 accgcgatgt gggggcgggc tatgtcccgc tggtgcgcgg cgggcatcgc gccttcgaca
     9781 ccgcgccgga ctgggcgatc cgctgggacg cggcggcgct ggcgcattat cgcaacgaca
     9841 agaaggcgcg ctttcagaac agcggctggt atttccgcgc cgggctggcc gtgccgatgg
     9901 tgacgtcgcg gcggctgtcg gcctcgctga tgcaggacgc ggtgttcgat cagggcgtgg
     9961 tcggggtctt tccgcgcgat ccggcgcagc gcgcggcgct tttgctgtac ctcaattcat
    10021 cggccgccac gcggctgcgc aacgaacggc tgggcggatc ggccaacaat tcggccaact
    10081 atctcaagcg tctgccggtc cccgccttcg acgccgccgc cgccgcgcgc gcgcagggcg
    10141 tcgtcgcgcg ggcgatccgg gacggcgacc tgccgcaggc cgtctgcgac gccttcgtcg
    10201 gcgatgcgtc aggcggcgcg gcttagcagt ggatagacgc ggcagcagtt gcgccccgcc
    10261 gccttggccg cgtacagcgc cttgtcggcc agcgcgacgc gctgcgccag atcgggctcg
    10321 cccgccgaac tccaatgcac gccgaagctg gccgtgaccg gggcgccgac gccgaagtcg
    10381 tggctctcga tgcaggcgcg cagcttctcc gccacgcgct gcgcgccgcg ccggttgacg
    10441 cccgcgcaga cgaccaggaa ctcctcgccg ccccagcgcg cggccagatc ggcggcgcgg
    10501 acatggcggc ggatgacccc ggccagatcg ctcagcaccc ggtcgccgca cgcatggcca
    10561 tgcgcgtcgt tcagcgcctt gaaatggtcg acgtccagca ggatcgcggc cagacccgcc
    10621 gtccgcccgc gcgccgccgt caggccgtcg aagcgctcgc ccatgccgcg ccggttgaga
    10681 aggccggtca gcgggtcgtg gttcgccgcc tcctgcgcct cgcgcagcgc cagcgcgtcc
    10741 agaatccgcc gctcgcgcag cgcgcgccgc aggttgacga cgcggtagcc gagatagccg
    10801 acgatcatca cgatccacac gcccagcagc agcgaatacc actgcgcctc gctcagcagc
    10861 gcccggcgca ccacgatttc gcgcacctga aaatcatgcc gccccagcgg cgcctcggag
    10921 ccggtttcga cgatcatcga ggtgatgtct ttgaactgcg gatggccgta gcgcggcggc
    10981 agacggcgtt tgcgcatcca ccattcggcg acgccgaaat cgtcgggcgt gaactcggcg
    11041 cgctgccatc gccccggcgt ggtgttggtt tcgacgccga gatattgcgg actttcatcg
    11101 tcggcgcggt cggcataggc cggatcgtag tttttcagat agacgcggaa cgaggtcgaa
    11161 gggccgccgt a
//
LOCUS       DASVLL010000468         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_461358,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000468 DASVLL010000000
VERSION     DASVLL010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461358"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VF402_07765"
     CDS             <1..1082
                     /locus_tag="VF402_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX7800232.1"
                     /translation="GAGNPDPLDLPDAVLVRPTVVCVFDNVAHEILLASAVWPDKLPA
                     QDAYSAALARLDAVEADLRAPLSAQPAPRDHHQEPLSSPTSAGQYGAMVERAKAYIAA
                     GDIFQVVPSHRFEAPYPLDPFAFYRSLRRHNPSPYLYYLNFGDFQLAGSSPEILVRVR
                     DGKLTIRPIAGTRPRGKTPAEDKALEEELLADPKELSEHLMLLDLGRNDVGRVAKPAS
                     VRVTEQFIIERYSHVMHIVSNVEGDAAETLDAVDALLAALPAGTLSGAPKVRAMEIID
                     ELESVKRGVVYGGGVGYISSGGAVDICIVLRTALFKDHMMYAQAGAGIVADSVPQTEY
                     EETCHKAGALIRAAQDAWRYSYFSN"
     gene            complement(1086..1367)
                     /locus_tag="VF402_07770"
     CDS             complement(1086..1367)
                     /locus_tag="VF402_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800233.1"
                     /translation="MTASSLDRLDQRLRAADSLLTRRRALPSGLALLAALAALVLSAL
                     LLIFLLTAGPKAIAAPAESAPKADFQLSGSAFPDAPSAPSIPSGSEPAR"
     gene            1515..2108
                     /locus_tag="VF402_07775"
     CDS             1515..2108
                     /locus_tag="VF402_07775"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7800234.1"
                     /translation="MILVIDNYDSFTYNLVHYLAELGAETQVYRNDALSVQDALALAP
                     RAILLSPGPCAPDQAGICLPLLRAAPEDMPILGVCLGHQSIGQAYGGDVIRAKTLMHG
                     KTSPIHHQGKGLFKNMPDPFTATRYHSLAVKRETLPADLEVTAWTEDGEIMGLQHRTR
                     PIHGVQFHPESIATEGGHQLLANFLDLAGVKTQNLYV"
BASE COUNT          369 a          741 c          682 g          328 t
ORIGIN      
        1 tgggcgcggg caatcccgat ccgctcgacc tgcccgacgc cgtgctggtg cgccccaccg
       61 tggtctgcgt gttcgacaat gtcgcgcacg aaatcctgct ggccagcgcg gtctggccgg
      121 acaagcttcc ggcgcaggac gcctacagcg ccgcgctggc gcgtctcgac gcggtcgagg
      181 ccgacctgcg cgcgccgctc agcgcccagc ccgcgccgcg ggaccaccat caggagccgc
      241 tgtcctcgcc gacctcggcg gggcaatacg gcgccatggt cgagcgcgcc aaggcttaca
      301 tcgccgcggg cgatatcttt caggtcgtgc ccagccaccg cttcgaggcg ccctacccgc
      361 tggacccgtt cgccttctac cgctccctgc gccgccataa tccgtcgccc tatctctatt
      421 acctcaattt cggcgacttc cagctggcgg gttcgtcgcc ggaaatcctg gtgcgggtgc
      481 gcgacggcaa gctgaccatc cgcccgatcg ccgggacgcg gccgcgcggc aagaccccgg
      541 ccgaggacaa ggcgctggaa gaggaactgc tggccgaccc caaggaattg tccgaacacc
      601 tgatgctgct cgacctcggc cgcaacgacg tcggccgggt cgccaaaccg gccagcgtgc
      661 gcgtgacgga gcaattcatc atcgagcgct attcgcacgt catgcacatc gtctcgaacg
      721 tcgagggcga cgcggcggag acgctggacg ccgtcgacgc gctgctggcc gccctgcccg
      781 ccggcaccct gtccggcgcg cccaaggtgc gggcgatgga gatcatcgac gagctggaat
      841 cggtcaagcg cggcgtcgtc tatggcggcg gcgtcggcta tatctcgtcc ggcggcgcgg
      901 tcgacatctg catcgtgctg cgcaccgccc tgttcaagga ccatatgatg tacgcgcagg
      961 cgggcgcggg catcgtggcc gacagcgtgc cgcaaacgga atacgaggaa acctgtcaca
     1021 aggcgggcgc cctgatccgc gcggcgcagg acgcctggcg ctacagctat ttcagcaatt
     1081 aacgcctacc gcgccggttc gctgcccgac ggtatggacg gcgcggacgg cgcatcggga
     1141 aacgcgctgc cggacaactg gaaatcggct ttcggcgcgc tttccgccgg ggcggcgatg
     1201 gctttcggtc cggccgtcag cagaaaaatc agcaacagcg cgctgagcac gagcgcagcc
     1261 agcgccgcca gcagggccag gcccgacggc agcgcgcgcc gccgcgtcag caggctgtcg
     1321 gcggcgcgta aacgctggtc gaggcggtcg agactggagg cggtcatagg cggcgtccgt
     1381 gaaagaatcg ccggtaaggc taggccgatg cggttaacga aaatttacat ttgcgccctg
     1441 atcgcccctt tgcgcatctt gccccttgga aaatcgggcg tatgtccgta atgagagcgt
     1501 aactggaggc cgccatgatc ctggtcatcg ataattacga cagcttcacc tacaacctcg
     1561 tccactatct ggccgagctg ggcgcggaaa cgcaggtgta ccgcaacgac gcgctcagcg
     1621 tgcaggacgc gctggcgctg gcgcccaggg cgatcctgct gtcgccggga ccgtgcgcgc
     1681 ccgatcaggc gggcatctgc ctgccgctgc tgcgcgccgc gccggaggac atgccgattc
     1741 tcggcgtctg cctcggccat caatccatcg gtcaggccta tggcggcgac gtgatccgcg
     1801 ccaagaccct gatgcacggc aagacctcgc cgatccacca ccaaggcaag ggcctgttca
     1861 aaaacatgcc cgacccgttc accgccacgc gctatcactc gctggccgtg aagcgcgaaa
     1921 ccctgcccgc cgatctggag gtcaccgcct ggaccgagga cggcgagatc atggggctgc
     1981 aacaccgcac gcgcccgatc cacggcgtgc agttccatcc cgaatcgatc gccaccgaag
     2041 gcggccatca gttgctggcc aacttcctcg atctggcggg cgtgaagacg caaaacctct
     2101 atgtctgacg ccttcaaacc
//
LOCUS       DASVLL010000469         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_352352,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000469 DASVLL010000000
VERSION     DASVLL010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352352"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="dusA"
                     /locus_tag="VF402_07780"
     CDS             complement(<1..915)
                     /gene="dusA"
                     /locus_tag="VF402_07780"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX7800235.1"
                     /translation="MTAKFSIAPMMDWTDRHCRAFHRALTQRALLYTEMVTAKAVIHG
                     DRERLIGFDAREHPVALQLGGSDPVELAQCAGIAAAWGYDEVNLNCGCPSDRVQSGAF
                     GACLMREPQLVADCMQAMREAAPIPATVKCRIGVDDQDPRTALFALVEACAPKQGRAG
                     VTTFIVHARKAWLKGLSPKENRDVPPLDYELVYELKRARPDLTISLNGGVTSLDAAEA
                     HLAHVDGVMLGRAAYHEPALLGQVDRRFFGVGEEVGPFAALDAYRPYMQAQLAAGTPL
                     AHMTRHMLGLMHGLPGARAFRRILTVDSI"
     gene            1299..2345
                     /locus_tag="VF402_07785"
     CDS             1299..2345
                     /locus_tag="VF402_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX7800236.1"
                     /translation="MASTAAQISGKIRLAINIGNTLEAQGGETGWGNPKITQALVNAY
                     KSAGFDAVRLPCAWDSYADKATARISDAWLERVRTVVQYCMNADLYVVLNIHWDGGWL
                     EKHVDAADKDAVGARQRAFWQQIATRLRDFDERLMFASANEPDASTAAGADILLNYHQ
                     IFIDAVRATGGRNACRTLIIQAPQTNIDKAVALWTHAPADPAADRMMLEVHYYDPSQF
                     TIITSDQSWGRMFYYWGKGNHSTIEPGRNATWGEEDYVDRQMRKLKTAFVDKGVPVIV
                     GEFGAYRKTGPLDMAKHNASVVAWNGYVVRQALANGASPFYWDTGGLIDRATCAVKDR
                     DVLDALLTAAGKKL"
     gene            complement(2340..>2877)
                     /locus_tag="VF402_07790"
     CDS             complement(2340..>2877)
                     /locus_tag="VF402_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7800237.1"
                     /translation="AGVAEVVRQGLADPARVCMLGVGFGGYAALKAAEGGHVRCAASI
                     DGVTDAASYLTWRQARAPSPDPDAFASLTPSAQWPRAFDDAPYSLRKLELFLGAGRPA
                     IDAAQVRAPVLLIHQAGDGVVPVSQSRALNDALRAAGKPVDFVELKGAGHAPDTEAAR
                     LAVLQSVTAFLARYDPPS"
BASE COUNT          495 a          977 c          959 g          446 t
ORIGIN      
        1 gatcgagtcc acggtcagga tgcgccggaa ggcccgcgcg cccggcaggc cgtgcatcag
       61 gcccagcatg tgccgcgtca tgtgcgccag cggcgtcccg gccgcaagct gcgcctgcat
      121 gtacgggcga taggcgtcca gcgccgcgaa cgggccgacc tcctcgccga cgccgaaaaa
      181 gcggcggtcg acctgcccca gcagcgccgg ttcgtgatag gcggcgcggc ccagcatcac
      241 cccgtcgaca tgcgccagat gcgcctcggc cgcgtcgagc gacgtaaccc cgccgttaag
      301 gctgatcgtc aggtcgggcc gcgcgcgctt caattcatag accagttcgt aatcgagcgg
      361 cgggacgtcg cggttctcct tcggcgacag gcccttcagc cacgccttgc gcgcgtggac
      421 gatgaaggtg gtcacgcccg cgcgtccctg ttttggcgcg caggcctcga ccagcgcgaa
      481 cagcgccgtg cgcggatcct gatcgtcgac gccgatgcgg cacttgaccg tcgccgggat
      541 cggagccgcc tcgcgcatgg cctgcatgca gtcggccacc aattgcggct cacgcatcag
      601 gcaggcgccg aacgcgcccg actgcacgcg gtcgctgggg cagccgcagt tgagattgac
      661 ctcgtcatag ccccacgccg ccgcgatccc ggcgcattgc gccagctcga ccggatcgga
      721 cccgcccagt tgcagcgcca cgggatgctc gcgcgcatcg aacccgatca gccgctcgcg
      781 atccccgtgg atcaccgcct tcgcggtcac catctcggta tagagcagcg cccgctgcgt
      841 cagcgcccgg tgaaacgccc ggcaatgccg gtcggtccag tccatcatgg gggcaatgct
      901 gaattttgcg gtcatatctg tctgattgtg aaggctattt atgcccggca cttccgtcct
      961 atctcggaag cgccgggctg tcccttgtcc caatacacga aaccgcgata tttatacaat
     1021 aaatttcagt atgttaaatg gccgcctgaa ggcggcggcg cgggcggttg aaaccgggtt
     1081 gcttatcggt gaagaagtct atgtattggg tcggcgccgc ttttccaggc gtgaaggcta
     1141 tcgcggggag acttatgagc atggaaagac gccggttctt atcgacggcg gcgggggccg
     1201 tcgcctgcgc cgtttatggc ggaagcgccg ccgcggaagc gccgtccggg ctttacccgg
     1261 actataatac cagccccatc cccgcggacg cgaccggcat ggcgagcacg gcggcgcaga
     1321 tttccggcaa gatccggctg gccatcaata tcggcaacac ccttgaggcg cagggcgggg
     1381 aaaccggctg gggcaatccg aagatcacgc aggcgcttgt caacgcctat aaatcggcgg
     1441 gcttcgacgc cgtccgcctg ccctgcgcct gggattcata cgccgacaag gcgacggcgc
     1501 ggatttcgga cgcctggctg gagcgcgtca ggacggtcgt tcagtattgc atgaacgccg
     1561 atctttatgt ggtcctcaac atccactggg acggcggctg gctggaaaag cacgtcgacg
     1621 cggcggacaa ggacgccgtc ggcgccagac agagggcgtt ctggcagcag atcgccaccc
     1681 gcctgcgcga cttcgacgaa cggctgatgt tcgccagcgc caacgaaccg gacgccagca
     1741 cggcggcggg ggcggacatc ctgctgaact atcaccagat ctttatcgac gccgtgcgcg
     1801 cgaccggcgg ccgcaacgcc tgtcgcaccc tgatcatcca ggcgccgcag acgaatatcg
     1861 acaaggccgt cgccctgtgg acgcatgcgc ccgccgatcc ggcggcggac cggatgatgc
     1921 tggaggtcca ttattacgac ccgtcgcaat tcaccatcat cacctcggat cagtcctggg
     1981 gcaggatgtt ctactactgg ggcaagggca atcactcgac gatcgagccg ggccgcaacg
     2041 cgacctgggg cgaggaggac tatgtcgacc ggcaaatgcg caagctcaag accgccttcg
     2101 tcgacaaggg cgttccggtc atcgtcggcg agttcggcgc ctatcgcaag accggcccgc
     2161 tggacatggc caaacacaac gcctccgtgg tcgcgtggaa cggctatgtc gtgcggcagg
     2221 cgctggccaa cggggcgtcg ccgttctact gggacaccgg cgggctgatc gaccgcgcga
     2281 cctgcgcggt caaggatcgg gacgttctgg acgccttact gacggcggcg ggcaaaaagc
     2341 tatgacggcg ggtcatagcg ggcgagaaac gccgtgaccg attgcaggac cgccagacgc
     2401 gccgcctccg tgtcgggcgc atggcccgcg cccttcagct cgacgaaatc gaccggcttg
     2461 cccgccgcgc gcagggcgtc gttcagcgcg cggctttgcg atacgggcac gacgccgtcc
     2521 ccggcctgat ggatcagcag cacgggcgcg cgcacctgcg ccgcgtcgat ggcgggccgc
     2581 ccggcgccca gaaacagctc cagcttgcgc aggctgtagg gcgcgtcgtc gaaggcgcgg
     2641 ggccattgcg ccgacggcgt caaactggcg aaggcgtccg gatcgggcga cggcgcgcgc
     2701 gcctgccgcc atgtcaggta gctggcggcg tcggtcacgc cgtcgatcga ggcggcgcag
     2761 cgcacatggc cgccttccgc cgccttcagc gcggcgtaac cgccgaagcc tacgccaagc
     2821 atgcagacgc gcgcggggtc ggccagcccc tgccgcacga cctcggccac gcccgcg
//
LOCUS       DASVLL010000470         4332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_352393,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000470 DASVLL010000000
VERSION     DASVLL010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352393"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VF402_07795"
     CDS             <1..741
                     /locus_tag="VF402_07795"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7800238.1"
                     /translation="AIGDAARLIRSGDADVMVAGGAESSIVPIGIAGFIACRALCTAF
                     NDNPKAASRPYDKDRDGFVMGEGAGIVVLEDYEHARARGAKIYAEVLGYGLSGDAYHI
                     TAPSEDGDGGFRAMKAAARNAGVDPKDIDYVNAHGTSTMADGIELKAIERFLGQRAAT
                     GTVSSTKSAIGHLLGGAGAVEAIFCTLAIRDQIAPPTLNLDNPDVETPIDLVPLKAKK
                     MKIDKAMSNSFGFGGTNAALILGKVADA"
     gene            743..1843
                     /gene="mltG"
                     /locus_tag="VF402_07800"
     CDS             743..1843
                     /gene="mltG"
                     /locus_tag="VF402_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7800239.1"
                     /translation="MTEAPAPKKVRSLLAPLAGLAAGVLLLIFAGGLFLYAELFGPGP
                     SPLTRVTFAHGMSVSVMAAQLQKQRVIRSATLFRVAARLRGRENGLKAGVYEFPARVS
                     LIGALSRIEDGKVVQTYVTIPEGRTSAQAVRILMAVKDLTGDVETPPEGSILPETYLY
                     TPGETRQAVLERMLAAGRKTLDELWARRAPGLPYASKEDALIMASIVERETAIPAERP
                     HIAAVFINRLKQGVRLGSDPTVIYGVSRGEPLGRGLSKAELETWTPWNTYLIDKLPVT
                     PICNPGKASIAAVLNPAQSNDLYFVANGAGGHVFSATYEQHLANVAKWRAVENQVDVY
                     TSSAVADADAPGRPAVATPQKAVATVKLKAER"
     gene            1847..2731
                     /locus_tag="VF402_07805"
     CDS             1847..2731
                     /locus_tag="VF402_07805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX7800240.1"
                     /translation="MALNSMTGFARIEGGHGDLNWTLELRSVNGRGLEVRYRFPAGFE
                     AVERIGRELSKRWFQRGQMNLTVAVTSARAATGVALNQQVLDLYVAASNRLVEAGHAL
                     TPGADGLLALRGVVETSGEDGAALDEAAEAALAADLASLCEGMAGARAAEGQALAAVL
                     SAHLDGMERLVAEAETLAAQQVEAIRDRFARRMAELSGEAGEGLAERVMQEAAALAVK
                     ADVREELDRLHSHIEQARRLIGQEVASGRKLDFLAQEFMREANTLCSKSALIELTRTG
                     LELKAVVDQFREQTQNVE"
     gene            2735..3376
                     /gene="gmk"
                     /locus_tag="VF402_07810"
     CDS             2735..3376
                     /gene="gmk"
                     /locus_tag="VF402_07810"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7800241.1"
                     /translation="MSIPQKLRRGLMLIVVAPSGAGKTSLCRRLMADHGDLDLSVSVT
                     TRGPRPGEQDGREYHFITDAAMDALVQDDALLEWAPVHDARYGTPREPVERALSQGKN
                     VLFDIDWQGAQRISVKAPSDVVRVFILPPSMLELRRRLVARAQDEMDVIERRLNRAKG
                     EIAHWAEYDYVILNDDFDRSYAELVHIYHAEVSRRSRGLWIAPFVDELMGEAI"
     gene            complement(3513..>4332)
                     /gene="rsmA"
                     /locus_tag="VF402_07815"
     CDS             complement(3513..>4332)
                     /gene="rsmA"
                     /locus_tag="VF402_07815"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7800242.1"
                     /translation="DLPTLRESLEQHGLMAKKAFGQHFLLDLNVTRKIVRLGGPFDGD
                     TVIEVGPGPGGLTRAVLETEAAHLIAVEKDARFIGLLEELKIPYGERFEVIEADALKV
                     DEARLLAERNLPPRAHIVSNLPYNVGTPLLIKWLTGPWRPLSMTLMFQLEVALRVVAP
                     VGDDDYGRLSVIAQVLCDCEKIMDLPARAFTPPPRVDSAVVRLTPKAERPDAVIVRNL
                     EKLTAAAFGQRRKMLRAALKALGGEALLAQAGIEPTLRAEQVAPADFLRLAGLL"
BASE COUNT          739 a         1402 c         1521 g          670 t
ORIGIN      
        1 gccatcggcg acgcggcccg gctgatccgt tcgggcgacg ccgacgtgat ggtggcgggc
       61 ggcgccgaat cgtcgatcgt gccgatcggc atcgcgggct tcatcgcctg ccgcgcgctc
      121 tgcaccgcgt tcaacgacaa tcccaaggcg gcgtcgcgcc cctatgacaa ggaccgcgac
      181 ggcttcgtca tgggcgaggg cgcgggcatc gtcgtgctgg aggactacga acacgccagg
      241 gcgcgcggcg cgaaaatcta cgccgaggtt ctgggctacg gcctgtccgg cgacgcctat
      301 cacattaccg cgccttcgga agacggcgac ggcggtttcc gcgccatgaa ggcggcggcc
      361 aggaacgcgg gcgtcgatcc gaaggacatc gattacgtca acgcgcacgg cacctcgacc
      421 atggccgacg gcatcgaact gaaggccatc gagcgattct tagggcaacg cgccgccacc
      481 ggcacggtgt cgtcgaccaa gtcggccatc gggcacctgc tgggcggcgc cggggcggtg
      541 gaggcgatct tctgcacgct ggccatccgc gaccagatcg cgccgccgac gctcaatctc
      601 gacaatcccg acgtcgaaac ccccatcgat ctcgtgccgc tcaaggccaa gaagatgaag
      661 atcgacaagg cgatgtccaa cagcttcggc ttcggcggca ccaatgcggc cctgatcctc
      721 ggcaaggtgg ccgacgcctg acatgaccga agcgcccgcg ccgaaaaagg tcagatcgct
      781 gctggcgccg ctggccggac tggcggcggg cgtgcttctg ctgatcttcg cgggcggact
      841 gttcctgtac gccgaactgt tcggccccgg ccccagcccg cttacgcgcg tcaccttcgc
      901 gcacggcatg agcgtctcgg tcatggcggc gcagttgcag aagcagcgcg tcatccgttc
      961 ggcgactctg ttccgcgtgg cggcgcggct gcgcggccgc gagaacggcc tgaaggcggg
     1021 cgtctatgaa tttcccgccc gcgtttcgct gatcggcgcg ctgagccgca tcgaggacgg
     1081 caaggtggtg cagacctatg tgaccatccc cgaaggccgc acctcggcgc aggccgtgcg
     1141 catcctgatg gccgtcaagg atctgaccgg cgacgtcgag acgccgccgg aaggctcgat
     1201 cctgccggaa acctatctct atacgccggg cgagacgcgg caggccgtgc tggagcgcat
     1261 gctggcggcg ggccgcaaaa cgctggacga actgtgggcc agacgcgcgc cgggcctgcc
     1321 ttacgccagc aaggaggacg cgctgatcat ggcgtccatc gtcgagcgcg aaacggccat
     1381 tccggcggag cgtccgcaca tcgccgccgt cttcatcaac cgcctgaaac agggcgtgcg
     1441 cctcggctcc gatccgaccg tgatctatgg cgtgtcgcgc ggcgagccgc tggggcgggg
     1501 gctgagcaag gcggaactgg aaacctggac gccgtggaac acctatctga tcgataagtt
     1561 gccggtgacg ccgatctgca atccgggcaa ggcgtccatc gccgccgtgc tcaatccggc
     1621 gcaaagcaac gacctctatt tcgtcgccaa cggcgcgggc gggcatgtgt tctcggccac
     1681 ctacgaacag catctggcca atgtcgccaa gtggcgcgcg gtcgagaatc aggtcgacgt
     1741 ctatacctcg tcggcggtgg cggacgcgga cgcgccgggc cgcccggcgg tcgcgacgcc
     1801 gcagaaggcc gtcgcgacgg tgaagctgaa agcggaaaga taggccatgg cgctgaacag
     1861 catgaccggg ttcgcccgca tcgaaggcgg gcatggcgac ctgaactgga cgctggaatt
     1921 gcgcagcgtc aacgggcgcg gcctcgaggt gcgctatcgc tttccggcgg gtttcgaggc
     1981 ggtcgagcgc atcgggcgcg aactgagcaa gcggtggttc cagcgcgggc agatgaacct
     2041 gaccgtcgcc gtgacgtcgg cgcgcgccgc cacgggcgtc gcgctcaatc agcaggtgct
     2101 ggacctctat gtcgccgcca gcaaccggct ggtcgaggcg ggccatgcgc tgacgccggg
     2161 cgcggacggg ctgctggcct tgcgcggcgt ggtcgagacg tcgggcgagg acggcgcggc
     2221 gctcgacgaa gccgccgagg ccgcgctggc cgccgatctg gccagtttat gcgagggcat
     2281 ggccggggcg cgcgccgccg aggggcaggc gctggcggcg gttctgagcg cgcatctgga
     2341 cggtatggaa cggctggtcg ccgaggcgga gacgctggcg gcgcagcagg tcgaggcgat
     2401 ccgcgaccgc ttcgcgcgcc gcatggcgga gttgagcggc gaggcgggcg aggggctggc
     2461 cgagcgcgtg atgcaggagg cggcggcttt ggcggtcaag gccgacgtgc gcgaggagct
     2521 ggatcgcttg cattcgcata tcgaacaggc gcggcggctg atcgggcaag aggttgcgtc
     2581 gggccgcaag ctcgatttcc tggcgcagga attcatgcgc gaggccaaca ccctgtgttc
     2641 gaaatcggcg ctgatcgaat tgacccgcac ggggctggag cttaaggcgg tcgtcgacca
     2701 gttccgcgaa cagacgcaaa atgtggagta aagcatgtcg atcccgcaaa aactgagacg
     2761 cggcctgatg ctgatcgtcg tggcgccgtc gggcgcgggc aagacgtcgc tgtgccgccg
     2821 cctgatggcc gatcacggcg atctggacct gtcggtttcg gtgacgacgc gcggcccgcg
     2881 tccgggcgag caggacgggc gcgaatatca tttcatcacc gacgcggcga tggacgcgct
     2941 ggtgcaggac gacgcgctgc tggaatgggc gccggtgcat gacgcgcgtt acggcacgcc
     3001 gcgcgagccg gtggagcgcg cgctgagtca gggcaagaac gtgctgttcg acatcgactg
     3061 gcagggggcg cagcgcatct cggtcaaggc gccgtcggac gtggtgcggg tgttcatcct
     3121 gccgccgtcg atgctggagc tgcgccgccg cctggtggcg cgcgcgcagg acgagatgga
     3181 cgtgatcgag cgccggttga accgcgccaa gggcgagatc gcgcactggg cggaatacga
     3241 ttatgtcatc ctcaatgacg atttcgaccg gtcttatgcg gagttggtgc atatctacca
     3301 cgccgaggtg tcgcggcggt cgcgggggct gtggatcgcg ccgtttgtag atgagctgat
     3361 gggcgaggcg atctgaaacc tctaatgggg cgtggggtca atgaccccac atctttcctt
     3421 cttcttcctt ttccgccacc gcccgaaggg cgatgacgga aaaggaaaag ggaggaaggc
     3481 tggggccatt ggccccagac cccgttacgg gttcagagca gcccggccaa ccgcagaaaa
     3541 tccgccggag cgacctgctc cgcgcgcaag gtcggctcga tccccgcctg agccagcagc
     3601 gcctcgccgc ccagcgcctt caaggcggcg cgcagcattt tccggcgctg gccgaaagcc
     3661 gcggcggtga gtttctccag attcctgacg atgaccgcat ccggccgctc cgccttcggc
     3721 gtcaggcgca ccacggcgct gtccaccctg ggcggcggcg tgaaggcgcg cgcgggcagg
     3781 tccatgatct tctcacagtc gcacaagacc tgcgcgatca ccgacaggcg accatagtcg
     3841 tcgtcgccga ccggcgccac gacgcgcaac gcgacctcca actggaacat cagcgtcatc
     3901 gacaacggcc gccacggccc ggtcagccac ttgatcagca aaggcgtgcc gacattgtaa
     3961 ggcaggttcg acacgatatg cgcccgcggc ggcagattgc gttccgcaag caatcgcgcc
     4021 tcatcgacct tcagcgcatc ggcctcgata acctcgaagc gttcgccgta agggattttc
     4081 agttcctcaa gcagcccgat gaagcgcgcg tccttctcga cggcgatcag atgcgcggcc
     4141 tcagtctcca gcacggcgcg cgtcaggccg ccggggccgg ggcccacctc gatcaccgta
     4201 tcgccgtcga aagggccgcc cagccgcacg atcttgcgcg tgacgttgag gtcgagcagg
     4261 aaatgctgcc cgaacgcctt tttcgccatc aggccgtgct gttccagact ctcgcgtaag
     4321 gtcggcagat cg
//
LOCUS       DASVLL010000471         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_191315,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000471 DASVLL010000000
VERSION     DASVLL010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191315"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VF402_07820"
     CDS             complement(<1..744)
                     /locus_tag="VF402_07820"
                     /inference="COORDINATES: protein motif:HMM:NF044765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX7800243.1"
                     /translation="MMPRPQVSLPPLYYVDHFHEMIDFVAATCAGLLGEAERAFIRDF
                     RALDAWAQCLYVRMINRRKTAFRPADLAYREIGDLHGGLAALTRAGFVRPLDARDHRA
                     VLSDLPKPDLLALAKAHGVAARAAWSKPQLTARLFEAVPFAALAAAQTDAIVPDRRDA
                     VDFLLYLQFGRSSDGMTRFALRDLGVAPTRAGSGHSPRFGSADEARAGFFYSRMLEAA
                     RQGGAVPDDGWPEPVSEDNRRLRDKLLHRL"
     gene            922..>2190
                     /locus_tag="VF402_07825"
     CDS             922..>2190
                     /locus_tag="VF402_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7800244.1"
                     /translation="MMSAGAHAATLEASGTLKADQPGPVIDKYVYSQFSEHLGRGIYD
                     GVWVGPESKIPNVRGVRSDIVAALKAIHTPAIRWPGGCFADEYHWRDGVGPRDQRPVR
                     KNHWWAGDAESNAFGTHEFMDFVEQVGADPYISINIGSSNPSEMLDWVDYMTSDGQDS
                     LAQERRKNGRDAPWKTPILGFGNEAWGCGGNMTPEYYSDELRKFTGFYHRDPNNPTIR
                     VASGANSDDTHWTDVVMKNAGHNIDALSLHYYTIPTGNWDHKGAATGFDEQAWTDTFA
                     QTLRMDKIISDHEAVMDRYDPQKRVGLFVDEWGTWYDVEPGTNPGRLYQQNTLRDAVL
                     AAANFNIFHHHADRVRLTAIAQTVNVLQSMILTEGDKIELTPTYYAYLMYVPFQDATE
                     LPLDIAAPTFTHGKTSIPAFNVSAARAKDGK"
BASE COUNT          412 a          739 c          707 g          332 t
ORIGIN      
        1 caggcggtgc agcagcttgt cgcgcaggcg ccggttgtcc tcgctgaccg gttcgggcca
       61 gccgtcgtcg ggaaccgcgc cgccctgacg cgccgcctcc agcatccggc tgtagaaaaa
      121 gcccgcgcgc gcttcgtccg ccgagccgaa acgggggctg tggcccgaac ccgcgcgggt
      181 cggcgcgacg cccagatcgc gcagcgcgaa ccgggtcatg ccgtcgctgg aacggccgaa
      241 ttgcagatag agcaggaagt ccacggcgtc gcgccggtcc gggacgatcg cgtccgtctg
      301 cgccgccgcc agcgccgcga acggcacggc ctcgaacagg cgcgccgtca gttgcggctt
      361 gctccacgcc gcgcgcgctg cgacgccgtg cgccttcgcc agcgccagca ggtcgggctt
      421 gggcaggtcg ctcagcacgg cgcgatggtc gcgcgcgtcg agcggccgga cgaaaccggc
      481 ccgcgtcagc gccgccagcc cgccatgcag gtcgccgatc tcccgatagg cgaggtcggc
      541 gggccggaac gcggtctttc tgcggttgat catgcgcacg taaagacact gcgcccacgc
      601 gtccagcgcg cggaagtcgc ggataaaggc gcgctcggcc tcgcccagca gtccggcgca
      661 ggtggcggcg acgaaatcga tcatctcgtg gaagtgatcg acataataga gcggcggcaa
      721 actgacctga ggccggggca tcatcgttct atatatgttc ctgacgctcc cggcaagcgt
      781 ctgaaaatcc cgcgccgccg cagttccggc tggtcaggcg cgatcaaatc ggttacagct
      841 tccgtcaata gtccgacaaa tagagattaa gaggaaatca tgaaacgact cctgaccgct
      901 ttcgtcgccg gcgccgcgct gatgatgagc gcgggcgcgc acgcagccac gctcgaagct
      961 tcgggaacgc tcaaggccga tcagccgggg ccggtcatcg acaaatatgt ctattcgcag
     1021 ttttccgagc atctggggcg cggcatctat gacggcgtct gggtcgggcc ggagtcgaag
     1081 attcccaatg tgcgcggcgt gcgctcggac atcgtcgcgg cgctgaaggc gatccacacc
     1141 ccggcgatcc gctggcccgg cggctgcttc gcggacgaat accactggcg cgacggcgtc
     1201 ggcccgcgcg atcagcgccc ggtgcgcaag aaccactggt gggcgggcga cgccgagagc
     1261 aacgccttcg gcacgcacga attcatggac ttcgtcgaac aggtcggcgc cgatccctat
     1321 atctcgatca atatcggctc ctccaatccc agcgagatgc tggactgggt cgactacatg
     1381 acctcggacg gtcaggacag ccttgcgcag gagcgccgca agaacggccg cgacgcgccg
     1441 tggaagacgc cgatcctcgg cttcggcaac gaggcgtggg gctgcggcgg caatatgacg
     1501 ccggaatatt attccgacga gctgcgcaag ttcaccggct tctatcaccg cgatccgaac
     1561 aatccgacga tccgcgtcgc ttcgggcgcc aattcggacg acacccactg gaccgacgtg
     1621 gtgatgaaga acgccgggca caatatcgac gccctgtcgc tgcattatta caccatcccg
     1681 accggcaact gggatcataa gggcgcggcg accggcttcg acgagcaggc gtggaccgac
     1741 accttcgccc agaccctgcg catggacaag atcatctccg accacgaggc ggtgatggac
     1801 aggtacgatc cgcaaaagcg cgtcggcctg ttcgtcgacg agtggggcac gtggtacgat
     1861 gtcgagccgg gcaccaatcc gggccgcctc tatcagcaga acaccctgcg cgacgccgtg
     1921 ctggcggcgg cgaacttcaa catcttccac caccacgccg accgggtgcg cctgaccgcc
     1981 atcgcccaga cggtcaacgt gctgcaatcg atgatcctga ccgaaggcga caagatcgag
     2041 ctgacgccga cctattacgc ttatctgatg tacgtgccgt tccaggacgc gacggaactc
     2101 ccgctcgata tcgcggcgcc gaccttcacg cacggcaaga ccagcatccc ggccttcaac
     2161 gtctcggcgg ccagggctaa ggacggcaag
//
LOCUS       DASVLL010000472         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_244863,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000472 DASVLL010000000
VERSION     DASVLL010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244863"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1400)
                     /locus_tag="VF402_07830"
     CDS             complement(309..1400)
                     /locus_tag="VF402_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7800245.1"
                     /translation="MSKRQSDYLSALLAEAPAQAPAPFPVQEASPASSSPAPSPASAA
                     PVPAKPGMTLLARESALARVAAGEVKQVTQLLLDPARVRIWPGNPRHQASLNEDNCRD
                     LIDAILAENGQKVPAIVRRVSGEAEFDYEVIAGSRRHWAISWLRANHYPDMMFLAQVH
                     ALDDEAAFRISDIENRARKDVSDFERARIYLKALDDHYGGRQARMAERLRLSKGWLSK
                     MLSVAALPGWIVDAFASPADIQLKTCYPLAQRVHAAQKDDADLLRALKAEARDLHKAQ
                     GQYEGQGVIPAAEVIRRLMSAGVDEAGREALVTLDKGGRAALSVVSANRNGVSVRLHA
                     GSGATPQELADMLRRALEDLDGQGRGLRF"
     gene            complement(1413..2651)
                     /locus_tag="VF402_07835"
     CDS             complement(1413..2651)
                     /locus_tag="VF402_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7800246.1"
                     /translation="MNANPPENGLLNAGFGGEAGRIGQLAGAGEAMVERLRRTAFLPG
                     AAKGLNVRLGIAEAADLLGCSTNRIRMAEQDGRLPPPPESESGRRLGYDIPSLLNMRK
                     VLGASPSRAPEDPPAIITVQNFKGGVGKSTVTTHLAHYLAVAGYRVLVVDCDSQATTT
                     TLFGFNPHFAIRREETLYPYRSIEPTEDDLRYAIKRTVWPNVDLIPSNLQMFDVEYEL
                     AATGSGGGGTLAARFRKLRQGLADLAQDYDVILLDPPPALGTISLAVMQAANALLVPL
                     AATTPDFCSTVQFLSMLSQVIQQLTEAGIKVEYDFCRLICSKFNSNDPSHTMIQRIME
                     QAFGPALLPVPILESAEISHAALRMMTIYELERPIGTPKTHKRCRSNIDEAMSQIEQL
                     IRQRWGRARPARAEDLVAAG"
     gene            complement(2845..>3081)
                     /locus_tag="VF402_07840"
     CDS             complement(2845..>3081)
                     /locus_tag="VF402_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800247.1"
                     /translation="DALPIRADARVLGATLKAGESAEYRLGQGRHAYLAPSSGALDVN
                     GVRIEARDGAAIKDEPVIRVTALEDAELVLVDAA"
BASE COUNT          476 a          993 c         1103 g          509 t
ORIGIN      
        1 catgacctcg cgctggtcgc gcaaggacag gtcggccaga tagggcagaa acaggtcgat
       61 ctgctccggc ttttcaccga cggctgcgcg catgccctct ccccttccct tccaacccgt
      121 ttccccaggg aaaccgccgc cgccgaaccc ggcggagggg aaaccacatg agtctgccta
      181 taccgattct cgcataacct ggagtcgcgc cgggtagaac cggctctacc gggcctgacc
      241 cgccggtaga gcctccattt tctctccgcc gggttcggcg ggggcggcgg tttccctggg
      301 gaaacgtttt agaagcgcag gcccctgccc tgcccgtcca gatcctccag cgcgcggcgc
      361 agcatgtcgg ccagttcctg cggcgtggcg ccggaaccgg cgtgcaggcg caccgagacg
      421 ccgttgcggt tggccgagac gacgctgagc gcggcgcgcc cgcccttgtc cagcgtgacc
      481 agcgcctcgc ggcccgcctc atcgacgccc gcgctcatca ggcggcggat gacctcggcg
      541 gcggggatca cgccctgacc ttcatattgg ccctgcgcct tgtgcaggtc gcgcgcctcg
      601 gccttcagcg ccctgagcag gtcggcgtcg tccttctggg cggcgtggac gcgctgcgcc
      661 agcgggtagc aggtcttgag ctggatgtcg gcggggctgg cgaaggcgtc gacgatccag
      721 ccgggcaggg cggcgacgct gagcatcttg gacagccagc ccttcgacag gcgcaggcgc
      781 tcggccatgc gcgcctgcct gccgccgtaa tggtcgtcca gcgccttgag atagatgcgc
      841 gcgcgctcga aatcggacac gtccttgcgc gcgcggttct cgatgtcgga gatgcggaag
      901 gccgcctcgt cgtcgagcgc atgcacctgc gccaggaaca tcatgtcggg atagtggttg
      961 gcgcgcagcc acgagatcgc ccaatggcgg cgcgaaccgg cgatgacctc gtagtcgaac
     1021 tcggcctcgc cggagacgcg gcgcacgatg gcgggcacct tctggccgtt ctcggccagg
     1081 atggcgtcga tcaggtcgcg gcaattgtcc tcgttgagcg aggcctgatg gcgcggattg
     1141 ccgggccaga tgcgcacgcg cgccggatcg agcagaagct gggtcacctg cttgacctcg
     1201 ccggcggcga cgcgcgccag cgccgattcg cgcgccagca aggtcatgcc gggcttggcc
     1261 ggaaccgggg ccgcggaggc gggggagggg gccggactcg atgaagcggg cgaggcttcc
     1321 tgaaccggga acggggcggg cgcctgcgcg ggcgcttcgg ccagcagggc ggacaggtaa
     1381 tcggactggc gtttcgacac gacgtggtct ccttaaccgg cggcgacgag gtcttcggcg
     1441 cgggcgggcc gggcgcggcc ccagcgctga cggatcagtt gctcgatctg cgacatggcc
     1501 tcgtcgatat tggagcggca gcgcttgtgc gtcttgggcg tgccgatcgg ccgctccagc
     1561 tcgtatatgg tcatcatgcg caaagccgca tggctgatct cggccgattc caggatcggc
     1621 acgggcagca gggccgggcc gaaagcctgc tccatgatgc gctggatcat ggtgtggctg
     1681 gggtcgttgg agttgaactt ggagcagatc aggcggcaga agtcgtattc gaccttgatg
     1741 cccgcctcgg tcagttgctg gatcacctgc gacagcatgg acaggaactg cacggtcgag
     1801 cagaagtccg gcgtggtcgc ggccagcggc accagcaggg cgttggccgc ctgcatgacc
     1861 gccagcgaga tggtgcccag cgcgggcggc gggtcgagca ggatcacgtc gtaatcctgc
     1921 gccagatcgg ccagcccctg acgcagcttg cggaagcgcg ccgccagcgt gccgccgccg
     1981 cccgaaccgg tggcggccag ctcgtactcg acgtcgaaca tctgcaggtt ggacgggatc
     2041 aggtcgacgt tcggccatac ggtgcgcttg atcgcatagc gcaggtcgtc ctcggtgggc
     2101 tcgatcgacc ggtacgggta cagcgtctcc tcgcggcgga tggcgaagtg cggattgaag
     2161 ccgaacaggg tggtggtggt cgcctggctg tcgcagtcga cgaccagcac gcgatagccc
     2221 gccacggcca gatagtgggc gaggtgggtg gtcacggtcg acttgccgac gccgcccttg
     2281 aagttctgta ccgtgatgat ggcgggcggg tcttccggcg cgcgcgacgg cgaggcgccc
     2341 agaaccttac gcatattgag cagggacggg atgtcgtagc cgaggcggcg gcccgattcg
     2401 ctttccggcg gcggcggcag gcgcccgtcc tgctcggcca tgcggatgcg gttggtcgaa
     2461 cagcccaaaa ggtcggcggc ctcggcgatg ccgaggcgca cgttcagccc cttggccgcg
     2521 ccgggcagga aggcggtgcg gcgcagacgc tcgaccatcg cctcgccggc gccggccaac
     2581 tggccgatgc gtccggcctc gccgccgaat ccggcgttca gcaggccgtt ctcaggcggg
     2641 tttgcgttca ttttagcctc cgatacacgg attgagtgaa ttttgcgtca tatttaacgc
     2701 taaagcttca atgcgccaag acggttaata aaaagctaat gacgccataa cccgattcgg
     2761 aaggcgtgcg gattcaaacc tgagacatga aaaagcgcgc gatccgtggg ggaagcgcgc
     2821 gcttttcgtg atgttacgcc gggatcaggc cgcgtcgacc agcaccagtt cggcgtcttc
     2881 cagcgcggtg acgcggatca cgggctcgtc cttgatggcg gcgccgtcgc gggcctcgat
     2941 gcggaccccg ttgacgtcga gcgcgccgga ggacggcgcg agataggcgt gacggccttg
     3001 gcccagcctg tattccgcgc tttcgcccgc cttcagcgtc gcgcccagca cgcgggcgtc
     3061 ggcgcggatc ggcagggcgt c
//
LOCUS       DASVLL010000473         6484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_191399,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000473 DASVLL010000000
VERSION     DASVLL010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191399"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VF402_07845"
     CDS             <1..541
                     /locus_tag="VF402_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7800248.1"
                     /translation="HPDVFNILLQVLDDGRLTDGQGRVVDFRNTLIVMTSNLGSDILV
                     NMEPDGDVEAVRPLVMNVVRAHFRPEFLNRIDEIILFHRLDQAHMHDIVRIQLKGLEK
                     LLADREMTLSIDEAALNDLAEKGFDPAYGARPLKRVIQKLLVDGIAREILAGRFVDGD
                     VIHVGFDGETLSIGKPTVN"
     gene            622..1281
                     /gene="pgsA"
                     /locus_tag="VF402_07850"
     CDS             622..1281
                     /gene="pgsA"
                     /locus_tag="VF402_07850"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7800249.1"
                     /translation="MANMRRTSQQAGDMTGESVNPIPNILTMIRLIGGVVMFVLLAGA
                     VGGIPFLDVNSEVLDALQRWGVYIFVIAALTDLFDGILARKLHAESRWGAILDPIADK
                     ILVCGTILGLFCIKTGDPVFALPAAFILFREFAVSALREAGAARGVKVPVSFMGKIKT
                     TLQLIALAALLVTESWGAFGLPLDFYGPCFIAANILLLIAAIASVWSGVAYFLAARAD
                     L"
     gene            1589..2728
                     /locus_tag="VF402_07855"
     CDS             1589..2728
                     /locus_tag="VF402_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7800250.1"
                     /translation="MSLALSTLIYEWRRYFAAIIALAVAGLLVLAMTGMFLGVGKAFT
                     ATIDRSPAEIMVLPAQADSLFGNNNGLPRRLIPTVYQHPDVTEVMPLDGSWAYWSNFP
                     KDGQPAKGNGVQVMVIDPGPGSLTIPNDFDERLIQTIAEPYAVVLDASSLGKLGVKVG
                     DKAKINGHTVWVRATTTDYPSMFNSMVFMTRQTAKLLYVVQEGPRVGGLLVKIKDPKR
                     VRQVVAELNKMGGGQYKAWTREDLSKVSERGMLKEGGISVMIGFAVIVGAFIGVVITW
                     QTLQAAILANIKEFAGLRALGVSMGSLRLIIMEISFWVGVFGLVVTALLVAGIAVLAR
                     AFNIPMDFPLYIDIPVSIVLLLIAIMSGFFSLGVLKKSQPADLLR"
     gene            2725..3435
                     /locus_tag="VF402_07860"
     CDS             2725..3435
                     /locus_tag="VF402_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7800251.1"
                     /translation="MTGQIAIDAKNLGKSYKIGKSKQQVLKNVDFNACHGQVTMIMGP
                     SGSGKSTLIAALSGLMKPDEGEVIALGENLWKKKRGKIDRFRLDHCGFIFQGFNLFPA
                     LNATQQVEQVLKYCKIPHGESKRRARAVLESVGLGPRLSLRPSEMSGGEKQRVAIARA
                     LSKEPSLIFADEPTSALDSVSGQVVIRLLHDAAKARNAAVIVVTHDPRLEAYADRIIH
                     MEDGKILSDHMVDRSSQA"
     gene            3447..4412
                     /locus_tag="VF402_07865"
     CDS             3447..4412
                     /locus_tag="VF402_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX7800252.1"
                     /translation="MSRKVFAVSALVLMAAAGGLAGCKPAPGGKGGKAAVTVPASNYA
                     AIANGKVDVEGGVIQVAARTAGIVREVYVQEGDTVAAGQILARQEDDAQRLAVQTARA
                     GVDQAAAAEQLTEVNIRTAQREYDRLAKLAPGNYVAKQKLDEAKDAIDTAKATLDSQV
                     AAVQTAKAQLAQAEYNLDLTVIRAPLAGKIIRRYANPGAGASTLNVSDMFDLEPAIPH
                     IIRAEIVESSIPDVHVGQEAEIVSENDPTKTYVGRVLRIAATFGARKLLSDSQNEATD
                     ERVVEVVVSADNAPFLIGQRVLVKFMKPGKKAQGTAAGGASASAG"
     gene            complement(4511..6070)
                     /locus_tag="VF402_07870"
     CDS             complement(4511..6070)
                     /locus_tag="VF402_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7800253.1"
                     /translation="MPENFDKDYKALLRPFGPRLWLTSAFAAGVSVCAVALLGLSGWF
                     LTAAAVAGAGGPVAVQAFNYLLPSAFIRFFAIGRTVLRYGERYLGHSAALRAMADLRP
                     ALFARLLGQRRGALMRLSRGEASSRFVQDVGALENRLVAQSAPASAAGGVVMTLLMCG
                     WASPWGALILLAFMAAALGAALYIHRRLPEADAASEQAAIGALKARFYELSALLPDVR
                     TYDLRAPLMAALRALEARLLAAKTHTAGRDAVTGAVSLILMGLCLVCLIASDAHVRLA
                     DLALSLLAASMGFDSLGVLAKAVGQKAGFDQARARVAALYDAVREPPPALPVKAPYLL
                     ADGARYDLDAGLRLRIDGASGSGKTRLIEGLMGLRDDRPGGVYDPSLFSLCPQDAALL
                     TGTVRDNLLMALRDAPPREAQEAMLWAALDDAALAERVRALPGGLDTWVGDGGVTLSG
                     GERKRLALARALLRDSPVLVLDEPTEGLDLATEAHVVARLRLRLKNRGLILISHREAP
                     RTLASQVLRAG"
     gene            complement(6070..>6484)
                     /locus_tag="VF402_07875"
     CDS             complement(6070..>6484)
                     /locus_tag="VF402_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7800254.1"
                     /translation="MYRRAILGLGYLPQETSIFRGLSVEKNILAVLELAEPDHAARAE
                     RLEQLLDEFGLTRLRTSPAMGLSGGERRRIGLARALLKNAPLWLLDEPAADLDAASEA
                     DLIARLPGLFEGRTVILSSHSPRLCALADRIVEVG"
BASE COUNT         1098 a         2216 c         2142 g         1028 t
ORIGIN      
        1 gcacccggac gtgttcaaca tcctgttgca ggtgctggac gacgggcggc tgaccgacgg
       61 gcaggggcgt gtggtggatt tccgcaatac gctgatcgtg atgacctcga acctcggttc
      121 ggacattctg gtcaatatgg agccggatgg cgatgtcgag gcggtgcgtc cgctggtgat
      181 gaatgtggtg cgggcgcatt tccgcccgga attcctgaac cgcatcgacg agatcatcct
      241 gttccaccgc ctcgatcagg cgcacatgca cgacatcgtg cgcatccagc tcaagggtct
      301 ggaaaagctg ctggccgacc gcgagatgac cttgagcatc gacgaggcgg cgctgaatga
      361 ccttgccgaa aagggcttcg accccgccta tggcgcgcgg cccttgaagc gggtgatcca
      421 gaagctgctg gtcgacggca tcgccaggga aatcctcgcc ggccgcttcg tcgacggcga
      481 cgtgatccac gtcggcttcg acggcgaaac cctgagcatc ggcaaaccga cggtgaacta
      541 acggggtccg gggccaatcg gccccggctc tttgcctgtt ttttccgcac gccggataaa
      601 aggcttggcg gggcggcggc gatggctaat atgcgccgga cttcacaaca ggcgggcgac
      661 atgaccggcg aaagcgtcaa tccgattccc aatatcctga ccatgatccg cctgatcggc
      721 ggggtcgtca tgttcgtgct gctggcgggg gcggtggggg ggattccgtt cctcgacgtc
      781 aattccgagg tgctggacgc gctgcaacgc tggggcgtct atatcttcgt catcgctgcc
      841 ctgaccgacc tgttcgacgg cattctggcg cgcaagctgc acgccgagag ccgctggggc
      901 gccatactcg acccgatcgc ggacaagatt ctggtctgcg gcacgatcct gggcctgttc
      961 tgcatcaaga ccggcgatcc ggtgttcgcc ctgcccgccg ccttcatcct gttccgcgaa
     1021 ttcgccgtca gcgccctgcg cgaagccggg gccgcgcgcg gggtcaaggt gccggtgtcc
     1081 ttcatgggca agatcaagac cacgctgcaa ctgatcgcgc tggccgccct gctggtcacc
     1141 gaaagctggg gcgcgttcgg cctgccgctc gacttttacg ggccgtgctt catcgccgcc
     1201 aatatcctgc tgctgatcgc cgccatcgcg tcggtctggt cgggcgtcgc ctacttcctc
     1261 gccgcccgcg cggatttgtg aggcgcctta tcttcccctc tcccctcgac gaggggagag
     1321 gacgagagcc gagcgcagcg aaggcgacgg gcggggcgcg cagcctgagg ggccgcccgc
     1381 aggttgagcg caaacgtcgg gcggcccctc acccgaggat cggcggctgc gccttgttcc
     1441 tcgtcctctc cccattgtca tggggagagg ggatccgggc gcaaaagatg tcaatatcgt
     1501 aaccaacctt accgcgattt aattggacct tcgcggtgaa gtccccgatg ctggccgccg
     1561 tcagttcgcg tttcgcgcag ggggagttgt gtccttagcc ttatcgaccc ttatctatga
     1621 atggcggcgg tatttcgctg ccattatcgc gctggccgtc gccgggctgc tggtgctggc
     1681 gatgaccggc atgtttctgg gcgtcggcaa ggcgttcacc gccaccatcg accgttctcc
     1741 ggccgagatc atggtgctgc ccgcgcaggc cgacagcctg ttcggcaaca ataacggcct
     1801 gccgcgccgc ctgatcccga cggtttacca gcatcccgac gtcaccgagg tcatgccgct
     1861 ggacggcagc tgggcctact ggtccaactt ccccaaggac ggccagcccg ccaagggcaa
     1921 cggcgtgcag gtcatggtca tcgaccccgg ccccggctcg ctgaccattc ccaacgattt
     1981 cgacgaacgg ctgatccaga cgatcgccga gccttacgcg gtcgtgctgg acgcctccag
     2041 cctcggcaag ctgggcgtca aggtcggcga caaggccaag atcaacggcc acaccgtatg
     2101 ggtgcgcgcc accacgaccg attatccgtc catgttcaac tccatggtct tcatgacgcg
     2161 ccagacggcc aagctgctct atgtcgtgca ggaaggcccg cgcgtcggcg gcctgctggt
     2221 caagatcaag gatcccaaac gcgtccgtca ggtcgtggcc gagctgaaca agatgggcgg
     2281 cggccagtac aaggcgtgga cgcgcgaaga cctgtccaaa gtgtccgagc gcggcatgct
     2341 gaaggagggc ggcatctcgg tcatgatcgg cttcgcggtg atcgtcggcg cctttatcgg
     2401 cgtcgtcatc acctggcaga ccttgcaggc cgccatcctg gccaatatca aggagttcgc
     2461 gggcctgcgc gcgctgggcg tgtcgatggg cagccttcgc ctgatcatca tggagatcag
     2521 cttctgggtc ggcgtgttcg ggctggtcgt cacggcgctg ctggtggcgg gcatcgccgt
     2581 gctggcgcgc gccttcaaca tccccatgga ctttccgctc tatatcgaca ttcccgtctc
     2641 catcgttctt ctgctgatcg ccatcatgtc gggcttcttc tcgctcggcg tcctcaagaa
     2701 gagccagccc gcggacctgc tgcgatgacc ggacaaatcg ccatcgacgc caagaatctc
     2761 ggcaagagct acaagatcgg caaatcgaag cagcaggtgc tgaagaatgt cgatttcaac
     2821 gcctgtcacg gtcaggtcac catgatcatg gggccgtccg gttcgggcaa gtcgaccctg
     2881 atcgcggccc tgtcggggct gatgaagccc gacgagggcg aggtgatcgc gctgggcgag
     2941 aacctgtgga agaagaagcg cggcaagatc gacaggttcc gcctcgacca ttgcggcttc
     3001 atctttcagg gcttcaacct gttcccggcg ctcaacgcca cgcagcaggt cgagcaggtg
     3061 ctgaaatact gcaagattcc gcacggcgaa tcgaagcgcc gcgccagggc cgtgctggaa
     3121 tcggtcgggc tggggccgcg cctgagcctg cgcccctcgg aaatgtcggg cggcgagaag
     3181 cagcgcgtcg ccatcgcccg cgcgctgtcg aaagagccgt cgctgatctt cgccgacgag
     3241 ccgacctcgg cgctcgattc ggtgtcgggg caggtggtga tccggctgct gcacgacgcc
     3301 gccaaggcgc gcaacgccgc cgtcatcgtc gtgacccacg atccgcgcct cgaagcctat
     3361 gccgaccgca tcattcacat ggaagacggc aagatcctga gcgatcacat ggtcgaccgt
     3421 tcttcgcaag cctaagagaa accttcatgt cacgcaaagt tttcgccgtt tccgccctcg
     3481 tcctgatggc cgccgccggg ggactggccg gctgcaagcc cgcgccgggc ggcaagggcg
     3541 gcaaggccgc cgtcaccgtg ccggccagca attacgccgc catcgccaac ggcaaggtcg
     3601 atgtcgaggg cggggtgatc caggtcgccg cccgcaccgc cggcatcgtg cgcgaagtct
     3661 atgtacagga aggcgacacg gtcgcggcgg gtcagattct ggcccgtcag gaggacgacg
     3721 cgcaacgcct ggccgtgcag accgccaggg ccggggtcga tcaggccgcc gccgccgagc
     3781 aactgaccga ggtcaacatc cgcacggcgc aacgcgaata cgaccgcctc gccaagctgg
     3841 cgcccggcaa ctatgtcgcc aagcagaagc tcgacgaggc gaaggacgcc atcgacaccg
     3901 ccaaggccac gctcgattcg caggtcgccg ccgtgcagac ggcgaaggcg caactggcgc
     3961 aggccgaata caatctcgac ctgaccgtga tccgcgcgcc gctggcgggc aagatcatcc
     4021 gccgctacgc caatccgggc gccggggcct cgaccctgaa cgtgtcggac atgttcgacc
     4081 tcgaaccggc gatcccgcac atcatccgcg ccgagatcgt cgaaagctcg atccccgacg
     4141 tgcatgtcgg tcaggaggcc gagatcgtgt cggagaacga tccgaccaag acctatgtcg
     4201 gccgcgtgct gcgtatcgcc gccaccttcg gcgcgcgtaa gctcctgtcc gacagccaga
     4261 acgaggcgac cgacgagcgc gtcgtcgagg tcgtggtcag cgccgacaac gcgcccttcc
     4321 tgatcggcca gcgcgtgctg gtcaagttca tgaagccggg caagaaggcg cagggcacgg
     4381 cggcgggcgg cgcttcggct tcggcgggct agagcgtttt ccgatctgat tgcatcagat
     4441 cggcgctcta actttatgct tttacgcgct ttttaatccg aaaagtgcgt cacacttttc
     4501 ggaaagcgtt ctaaccggcc ctcaacacct gagacgccaa cgtgcgcggc gcctcgcggt
     4561 ggctgatcag gatcaggccg cggttcttga gccgcagccg cagccgcgcg accacgtgcg
     4621 cctcggtggc gaggtccagc ccctcggtcg gttcgtccag caccagaacc ggcgagtcgc
     4681 gcagcagcgc gcgcgccagc gccagccgct tgcgctcgcc accggataag gtcaccccgc
     4741 cgtcgccgac ccacgtgtcg aggccccccg gcagggcgcg cacccgctcc gccagcgcgg
     4801 cgtcgtccag cgccgcccac agcatcgcct cctgcgcctc gcgcggcggg gcgtcgcgca
     4861 gcgccatcag caaattgtcg cgcaccgtgc cggtcaagag ggcggcgtcc tgcgggcaca
     4921 ggctgaacag ggacggatcg taaacgccgc cgggccggtc gtcgcgcaag cccatcagcc
     4981 cttcgatcag ccgcgtcttg ccgctgcccg acgcgccgtc gatgcgcagc cgcaaaccgg
     5041 cgtcgagatc gtagcgcgcg ccgtccgcca gcaggtacgg cgctttaacc ggcaacgcgg
     5101 gcggcggctc gcgcacggcg tcgtacaggg cggcgacgcg cgcccgcgcc tgatcgaagc
     5161 ccgccttctg gccgacggcc ttcgccagga cgccgaggct gtcgaagccc atgctggccg
     5221 ccagcaggct cagcgccaga tcggcgaggc ggacatgcgc gtccgacgcg atcaggcaga
     5281 cgaggcacag ccccatcagg atcaggctga ccgccccggt gacggcgtcg cgcccggccg
     5341 tatgcgtctt cgccgcgagc agccgcgcct ccagcgcgcg cagcgccgcc atcagcggcg
     5401 cgcgcaggtc gtaggtgcgc acgtcgggca gcagggcgct cagttcgtag aaccgcgcct
     5461 tgagcgcgcc gatggcggcc tgttccgacg ccgcgtcggc ttcgggcagg cggcggtgga
     5521 tatagagcgc cgcgcccagc gccgccgcca tgaaggccag caggatcagc gccccccacg
     5581 ggctggccca gccgcacatc agcagcgtca tgacgacgcc gcccgccgcc gaggccgggg
     5641 cgctttgcgc gaccagccgg ttttccagcg cgccgacatc ctgcacgaaa cggctggacg
     5701 cctcgccgcg cgacaggcgc atcagcgcgc cgcgccgctg gcccagcagg cgcgcgaaca
     5761 gggcggggcg caggtcggcc atggcgcgca gggccgcgct gtggccgaga tagcgctcgc
     5821 cgtagcgcag cacggtgcgg ccgatggcga agaagcggat gaaggcgctg ggcagcaggt
     5881 agttgaacgc ctgcacggcg acggggccgc ccgcgcccgc cacggcggcg gcggtcagga
     5941 accagccgga caggcccagc aaggccacgg cgcacacgct cacccccgcc gcgaaggccg
     6001 aggtcagcca caggcgcggc ccgaacgggc gcagcaaagc tttgtaatcc ttatcgaagt
     6061 tttcaggcat cagccgacct ccacgatacg gtcggccagc gcgcacaggc gcggcgaatg
     6121 gctggacagg atcacggtgc ggccctcgaa caggccgggc aggcgcgcga tcaggtcggc
     6181 ttcggacgcc gcgtcgaggt ccgccgccgg ctcgtccagc agccacaggg gcgcgttctt
     6241 caaaagcgcg cgcgccagcc cgatgcgccg ccgctcgccg cccgacaggc ccatcgccgg
     6301 cgaggtgcgc agtcgcgtca gcccgaattc gtccagcaat tgctccagcc gttccgcgcg
     6361 cgccgcatgg tccggctccg ccagctccag cacggcgagg atgttctttt cgaccgacag
     6421 gccgcggaag atcgacgtct cctgcggcag atagccgagg ccgaggatcg cacggcggta
     6481 catc
//
LOCUS       DASVLL010000474         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_301111,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000474 DASVLL010000000
VERSION     DASVLL010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301111"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF402_07880"
     CDS             complement(<1..253)
                     /locus_tag="VF402_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7800255.1"
                     /translation="MRDIHHFIDGRAVPGTSGRFGDVYDPNTGRVQARVALASPAEVD
                     DAVWRAAKAQTGWAAVNPQRRARVMFAFKALLESHMDELA"
     gene            complement(329..1402)
                     /locus_tag="VF402_07885"
     CDS             complement(329..1402)
                     /locus_tag="VF402_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX7800256.1"
                     /translation="MFDHPDYDGHEKVLMAEDAQSGLRAIIAVHSTALGFAAGGCRLW
                     RYATSEAALSDALRLSRGMSYKNAMADLPLGGGKAVILGPVAPERRQAAFEAFGRMVD
                     SLNGAYVTAEDVGVSVADMQVVARTTPYVSGLSGAEGRSGGDPSPYTARGVRVGIEAA
                     ARARLGRPEVAGLAVAVQGLGHVGMSLCRELHDLGANLVVSDIDAGRVQAAVARFGAR
                     AVGADDILTQDVDVVAPCALGGAISEYAARYVKAGIVAGAANNQLATPEAGRILAERG
                     VLYAPDYVINAGGIIMVSGELSGDNDEAQVLARVDAIGARLDAIFAAAKAENVITNEV
                     ADRMARAKIAAGKPARAPVPTTA"
     gene            complement(1395..>2260)
                     /locus_tag="VF402_07890"
     CDS             complement(1395..>2260)
                     /locus_tag="VF402_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEX7800257.1"
                     /translation="RSYLGDAVFSLMVMLGAAWQSGALPLTRAAVEKAIALNGAAVKA
                     NLGAFALGRAWAEGRLDATLKPEPDFDLEAFTARRMADLTAYQNAAYAAQYKALVDLA
                     RKAEAPLHDARFSEATARYAFKLMAYKDEYEVARLYGLPEFRRRLAEQFEGTKAVHVW
                     LAPPLLGDKKRRFGPWVFGLFAVLRTLKGLRGTPFDPFGHTPERRRERALIAEYAALI
                     RALAPRLAAHNLDLAVALARLPDDIRGFGPIKTANADKAEARRAELKAQFDAARPAVR
                     NVMPAKETEHV"
BASE COUNT          332 a          835 c          752 g          341 t
ORIGIN      
        1 gcgccagttc gtccatatgg ctttccagca gcgccttgaa ggcgaacatg acgcgcgcgc
       61 gccgctgcgg attcacggcg gcccagccgg tttgcgcctt cgccgcccgc cacacggcgt
      121 cgtcgacctc ggcagggctc gccagcgcca cgcgcgcctg aacccggccg gtgttgggat
      181 cgtacacgtc gccgaaacgg ccggacgttc ccggaacagc ccggccgtcg atgaagtgat
      241 gaatgtcgcg catgatgacg tcctgaagcg gcgggcgccc ctcccgccgc ttcgcggtcc
      301 ccctctccgt tgcgcaggga gggatgactt acgcggtcgt cgggacggga gccctggcgg
      361 gttttccggc ggcgatctta gcgcgcgcca tgcgatcggc aacctcattt gtgatcacgt
      421 tttcagcttt cgccgccgcg aagatggcgt cgagccgcgc gccgatggcg tcgacgcgcg
      481 ccagcacctg cgcctcgtcg ttgtcgccgg acagctcgcc cgacaccatg atgatgccgc
      541 ccgcattgat cacatagtcc ggcgcgtaca ggacgccgcg ttcggccagg atgcggcccg
      601 cctccggcgt cgccaactgg ttgttggccg cgcccgcgac gatgccggcc ttgacgtagc
      661 gcgcggcata ttcgctgatc gcgccgccca aagcgcacgg cgcgaccacg tcgacgtcct
      721 gcgtcaggat atcgtcggcg ccgaccgccc tggcgccgaa gcgcgccaca gccgcctgca
      781 cgcgcccggc gtcgatgtcc gacaccacca gattggcgcc gagatcgtgc agttcccggc
      841 acagggacat gccgacatgg ccgaggccct gcaccgcgac ggccagaccg gcgacttccg
      901 ggcggcccag ccgcgcccgc gccgccgcct cgatgccgac gcgcacgccg cgcgcggtgt
      961 agggcgacgg atcgccgccg gaacggccct cggcgccgct caggcccgat acgtaaggcg
     1021 tggtccgcgc cacgacctgc atgtccgcca ccgacacgcc gacatcctcg gcggtgacgt
     1081 aggcgccgtt caggctgtcc accatgcgcc cgaaggcttc gaacgccgcc tgacggcgct
     1141 ccggcgcgac ggggccgagg atcaccgcct tgccgccgcc cagcggcaga tcggccatcg
     1201 cgttcttgta gctcatgccg cgcgacaggc gcagcgcgtc cgacagggcc gcctcgctcg
     1261 tcgcatagcg ccatagacgg caaccgcccg ccgcgaagcc cagcgcggtc gaatgcacgg
     1321 cgatgatggc gcgcaggccg ctttgcgcgt cctccgccat cagcaccttt tcgtggccgt
     1381 catagtcggg atggtcaaac atgctcggtt tcctttgctg gcatgacgtt acggaccgcc
     1441 gggcgggcgg cgtcgaattg cgccttgagt tcggcgcgtc tggcctcggc cttgtcggcg
     1501 ttggcggtct tgatcgggcc gaagccgcga atgtcgtcgg gcaggcgggc caaagccacg
     1561 gcgaggtcga gattgtgcgc ggccagacgc ggcgcgaggg cgcggatcag cgcggcgtac
     1621 tccgcgatca gcgcgcgttc gcggcggcgc tccggcgtat ggccgaacgg atcgaacggc
     1681 gtgccgcgaa gccctttcag cgtccgcagc acggcgaaca ggccgaacac ccacgggccg
     1741 aacctgcgct ttttgtcccc cagcagcggc ggcgccagcc agacatgcac cgccttcgtc
     1801 ccctcgaact gttcggccag gcgccggcgg aactccggca ggccgtagag gcgcgccacc
     1861 tcgtattcgt ccttataggc catcagcttg aaggcgtagc gcgccgtcgc ctcgctgaaa
     1921 cgcgcatcgt gcagcggcgc ttcggccttg cgcgccagat cgaccagcgc cttatattgc
     1981 gcagcatagg cggcgttctg ataggcggtc aggtccgcca tgcggcgcgc agtgaaggct
     2041 tccagatcga aatccggctc cggctttagc gtggcgtcca gccgcccttc cgcccacgcc
     2101 cggcccagcg cgaaggcgcc gagattggcc ttcaccgccg cgccgttcaa cgcgatcgcc
     2161 ttttcgaccg ccgcgcgcgt cagcggcagg gcgcccgact gccaggccgc gcccagcatc
     2221 accatcagcg aaaacaccgc gtcgccgaga tagctccgcg
//
LOCUS       DASVLL010000475         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_84810, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000475 DASVLL010000000
VERSION     DASVLL010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84810"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VF402_07895"
     CDS             complement(<1..434)
                     /locus_tag="VF402_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7800258.1"
                     /translation="MKKTLIIAAGVAASLALAGVASAQDVGQVYGSLGYQNSNNDKTD
                     SNLGSVDARVGTKLNRYFGVEGEAAFGTNHDDTAAGRYKLTNKWGAYGVGYLPVSPSF
                     DLIGRVGVSDTNLKAPPAAGKLEQGTALDYGVGAQYHLNKDYA"
     gene            complement(699..1163)
                     /locus_tag="VF402_07900"
     CDS             complement(699..1163)
                     /locus_tag="VF402_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEX7800259.1"
                     /translation="MRLASIDIDEQSLSAASDQIEHERKVAVFDLIEKNSFAPEGAAE
                     AGCEGPFGLRLALEDNRLAFDVSGPGYARRILLSLTPFKTIFRDYRMICESYYEALKN
                     ATPAQIEAIDMGRRGVHNEGAELLSERLSGKIDIDHETSRRLFTLINALVRS"
     gene            complement(1240..1617)
                     /locus_tag="VF402_07905"
                     /pseudo
     CDS             complement(1240..1617)
                     /locus_tag="VF402_07905"
                     /inference="COORDINATES: protein motif:HMM:NF022612.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
     gene            complement(1618..2916)
                     /gene="murA"
                     /locus_tag="VF402_07910"
     CDS             complement(1618..2916)
                     /gene="murA"
                     /locus_tag="VF402_07910"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7800260.1"
                     /translation="MDRIAIVGGSPLFGDIPISGAKNSAIKLMAASLLTSEPVRLTNM
                     PRLADTRLLSRLLQRFGCGVSETDGPDGQEMRLHTPEITSAFAPYDLVRQMRASFNVL
                     GPLLARTGHAKVSLPGGCTIGARPVDLHLKALEALGAHIDMHEGYVFAQAPRGLQGAE
                     IEFPFVSVGATEHALMAAALAHGTTVLKNSAREPEIVDLAECLNAMGARISGAGTPTI
                     AIEGVTSLRGTDWAVICDRIEAGTYAAAGAMAGGEVRLTRVRPELIAILLDKLEAAGC
                     EVHRGFDTVTIRRKDGQRLKPVDVTTGVYPGFATDLQAQFMALMTLADGESTIRETIF
                     ENRFMHVPELGRLGADISVSGGEAVVRGVAELRGAQVMATDLRASACLVIAGLAARGE
                     TVINRVYHLDRGFENLEGKLSACGANVRRLKGESGPAEDE"
     gene            complement(3028..3192)
                     /locus_tag="VF402_07915"
     CDS             complement(3028..3192)
                     /locus_tag="VF402_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800261.1"
                     /translation="MSKKTGTAPPKTAAEQRAERLQAALRDNLRRRKTPGKAAQAAPE
                     PDPDGDGPED"
     gene            3299..3373
                     /locus_tag="VF402_07920"
     tRNA            3299..3373
                     /locus_tag="VF402_07920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3331..3333,aa:Thr,seq:ggt)
     gene            complement(3560..>3851)
                     /locus_tag="VF402_07925"
     CDS             complement(3560..>3851)
                     /locus_tag="VF402_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800262.1"
                     /translation="QKADKNTRALVIASLILQQPTHESAARLIHDCSFGYKLDSLNVP
                     NIALAVQRNNKACATMEKIFSDSAVEIQLFSPISKVPSDDKGQRKQGLSCEE"
BASE COUNT          623 a         1273 c         1226 g          729 t
ORIGIN      
        1 gcgtagtcct tgttgaggtg atactgggcg ccgacgccgt aatcgagggc cgtgccctgc
       61 tccagcttgc ccgcggcggg cggcgccttc aggttggtgt ccgagacgcc gacgcggccg
      121 atcaggtcga agctgggcga caccggcaga tagccgacgc cataggcgcc ccacttgttg
      181 gtcagcttgt agcggcccgc ggcggtgtcg tcgtggttcg tgccgaaggc ggcctcgccc
      241 tcgacgccga aatagcggtt gagcttggtc ccgacgcgcg cgtcgacgct gcccagattg
      301 gagtcggtct tgtcgttgtt gctgttctga tagccgagcg agccgtaaac ctgaccgacg
      361 tcctgcgccg aagcgacgcc cgcaagagcc agagacgcgg cgacgcccgc cgcaatgatg
      421 agggtcttct tcatgatggt gtgctccata tttcgttacc ggcgatcgtt tcgtcgcctg
      481 agagaaatat ggaggcgccg cccggccttc atggggcgtt tccgaggtcg ttttggggta
      541 ttcgggttca attgcggcgg aaaggacgcg attgcgcccc agatgcggcg ggatgtccgc
      601 tttccgccgg attgaggcca tgtagatgaa cctcagatga acggccgttc attacaaaaa
      661 tgtaagaaaa tcataaggat aggcagattc tgccgacctc agctccgcac cagcgcgttg
      721 atcagcgtga acaggcggcg cgacgtttca tggtcgatgt cgatcttgcc gctcagccgc
      781 tcgctcagca gttcggcgcc ttcgttatgc acgccgcggc ggcccatgtc gatcgcctcg
      841 atctgggcgg gcgtggcgtt tttcagcgcc tcgtaatagc tttcgcagat catgcggtag
      901 tcgcggaaga tggtcttgaa cggcgtcagc gacagcagga tgcggcgcgc atagcccggc
      961 ccgctcacat cgaaggccag ccggttgtcc tccagcgcca gccgcaggcc gaacgggcct
     1021 tcgcagcccg cctccgccgc gccttcgggc gcaaaactgt tcttctcgat caggtcgaag
     1081 accgccacct tgcgctcgtg ctcgatctgg tcggacgcgg cggaaaggct ctgttcgtcg
     1141 atgtcgatgc tggcgaggcg catgatgaca gtccgtgggg tggtccggcg catggcgcgc
     1201 gcggcgcggc gcggcgaggt cgagcagggt caggtcgacg cattcggcct cgatgcgcac
     1261 atccttagcc ccctcgcccg cgaagcgcaa tgtcagcgcg accgccggat cgtccagcgt
     1321 ctcgaccgcg atgtcgagca ggctgagcgg cgtcgcggcg cttttgggat cgaagccgcg
     1381 cgcctgcacc cgcaggaccg acgcgatgcg cagcgccgac ggcgcgcgca aggcgcggcc
     1441 cggtctttcg tgacagaagc ggctcatgcc cagcgtgaaa tgccgcgccc cggcgtcgta
     1501 ggccagatcg cccgcgcgca gcgccgcgtc ctgcgtcagc gcggccagcg ccgccacgtc
     1561 ctccggcgcc tgcgcgatca gccgcagcgg cctgtgcggt ttcccccggc cgaacatcta
     1621 ttcgtcttcc gccgggccgg actcgccctt gaggcggcgc acgttcgcgc cgcaggccga
     1681 cagcttgccc tcaagattct cgaagccgcg atcgaggtga tagacccggt tgatgacggt
     1741 ttcgccgcgc gccgccagcc cggcgatcac caggcaggcc gaggcgcgca gatcggtggc
     1801 catgacctgc gctccgcgca gctcggcgac gccgcgcacc accgcctcgc cgccgctgac
     1861 cgaaatgtcg gcgccgaggc ggcccagttc ggggacatgc atgaagcggt tttcgaaaat
     1921 cgtctcgcgg atggtcgatt cgccgtcggc cagcgtcatc agcgccatga actgcgcctg
     1981 cagatccgtc gcgaagcccg gatagacgcc ggtggtcacg tcgaccggct tcaggcgctg
     2041 gccgtccttg cgccggatcg tcaccgtgtc gaagccgcga tgcacctcgc agcccgccgc
     2101 ctccagcttg tcgagcagga tcgcgatcag ttccggccgc accctggtca gccgcacctc
     2161 gccgcccgcc atcgcgccgg ccgccgcata ggtgcccgcc tcgatgcggt cgcagatcac
     2221 ggcccagtcg gtcccgcgca ggctggtcac gccctcgatg gcgatggtgg gcgtgcccgc
     2281 gcccgaaatc ctggcgccca tcgcgttcag gcattcggcc agatcgacga tctccggttc
     2341 gcgcgcgctg tttttcagca ccgtcgtccc gtgcgccagc gccgccgcca tcagcgcgtg
     2401 ctcggtggcg ccgacgctga cgaaggggaa ttcgatctcg gcgccttgca ggccgcgcgg
     2461 cgcctgggcg aagacatagc cctcgtgcat gtcgatatgc gcgcccagcg cctccagcgc
     2521 cttcaaatgc aggtccacgg gccgcgcgcc gatggtgcag ccgccgggca agctgacctt
     2581 ggcgtggccg gtgcgcgcca aaagcggccc cagcacgttg aacgaggcgc gcatctgccg
     2641 caccagatcg tagggcgcga aggccgaggt gatttccggc gtgtgcagcc gcatctcctg
     2701 cccgtccggc ccgtccgtct cgctgacgcc gcagccgaag cgctgcaaca ggcggctcaa
     2761 caggcgcgta tcggccagac gcggcatatt ggtcaggcgc accggctcgg aggtcagcag
     2821 cgacgccgcc atcagcttga tggccgaatt cttggcgccg gaaatgggga tgtccccgaa
     2881 caaggggctg ccgcccacga tagcgatgcg atccataaaa ccagtcgtcc gtccactcat
     2941 ccgcacaaca gcggaaacag cggttctaca tgaccttgtg cgccgcgcaa cgacttttgc
     3001 gcgcggacgc ggctcgcaat cgcgtgatca gtcctccggc ccgtcgccgt cggggtccgg
     3061 ctccggcgcg gcctgcgccg ccttgcccgg cgtctttctg cggcgcaggt tgtcgcgcag
     3121 cgccgcctgc agacgctcgg cgcgttgctc ggcggcggtt ttcggcggcg cggttccggt
     3181 tttcttcgac atgcggcgtg atccttcgcg cctgtcctaa aaaatgcggg gaataattgc
     3241 aagtccgggc atttttcgct ttgccttcgc aaaaagtttg gctatgacgc acgccttcgc
     3301 cgccgtagct cagtggtaga gcgcatcctt ggtaaggctg aggtcggcag ttcaatcctg
     3361 cccggcggca ccatcggaac ccaagccctg caaagggttt gggttccgac cacacctccc
     3421 gacatttatg caaatttacg cgtttcgtag gcattctgct tcttcgacca tatctcatag
     3481 gacggcatca ttcgtattgc catcccgctc ccaatggaac atgaatacgc gcgttcctat
     3541 ccgactaata ccacggtgcc tattcctcac acgacagtcc ctgcttgcgt tgtcctttat
     3601 catcgcttgg caccttagaa atcgggctga ataactggat ttctacggcc gagtcagaaa
     3661 atattttctc catagtggcg caggctttat tgtttctttg cactgccagt gcgatattgg
     3721 gaacattcag agagtcgagc ttgtacccaa atgagcaatc atgtataagc cgcgccgccg
     3781 attcgtgtgt gggctgctgg agtattaagg acgctatgac cagagcacgt gtatttttgt
     3841 cggccttttg g
//
LOCUS       DASVLL010000476         3690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_30758, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000476 DASVLL010000000
VERSION     DASVLL010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30758"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1566)
                     /locus_tag="VF402_07930"
     CDS             complement(241..1566)
                     /locus_tag="VF402_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800263.1"
                     /translation="MTAPSLSRAQDAAPRLIKPAVFGEVREVTGDAVPLVAAKPGCKL
                     APLKDGPCLDGIALDATPSRVLMLARPAQPGARVAGAYGRDFTLYTVWRDARGAQAQA
                     LPLPTSGLSVPRDCFALDGEDVGYGIGSRNGGLVAVESQIVSCGGGPRAPHGPYAPQG
                     DPIRSGPGGWHVTEMRLVAGQRRYLAQPGSCDPRFSVKTDWCAETAVRYLQSHPDEKE
                     LDLIATRAAARAGDVLGDDDIQQWVLKRKGDAKFKADSRWFKTSMMTAVDGCAPVEAV
                     RWYVEPGEDGLYITEKALNRCGAPPAPVPTETYEAYGQDMFIVDCARHRWRDRDEKSP
                     DGCFDQARGYLQRTRQSSAFVVVLNQEARLDDHLYDGGYISYDVARVSLGKDGALMAK
                     RDTVYTPGVALRNCSAVGDDPAESRGFVIVRSMGVQWARPYVWMRCPVY"
     gene            complement(1691..3166)
                     /locus_tag="VF402_07935"
     CDS             complement(1691..3166)
                     /locus_tag="VF402_07935"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7800264.1"
                     /translation="MSAVRLSDILRRDLDRDPVVTGVTADSRKVADGTLFCALPGAKA
                     DGRDFIPQAIRQGAAAILAPDGPFGKGLGVEDGESVVVQVADVRRAYALAAKAFYGAQ
                     PKTCVAVTGTNGKTSVATFCRQIFAALGHTTGSMGTLGVVRQNPDGTETAITPPGLTT
                     PDAADMARHMAELAQDGVTHVALEASSHGIDQRRLDGVSLCAAGFTNLTQDHLDYHAT
                     MDAYRTAKLRLFEQLLPRGRTAVLNADSDGYNAFAAMSILSGLSVMAVGERGRGLALT
                     GRDLTVDGQILYLEHQGRTYDVKLPLAGLFQASNALVAAGLAIAAGEEAGAVIAALGR
                     LKGARGRLERVGAKGNGAEVYVDYAHTPDGLETVLKALRPHTHGRLVCVFGCGGDRDR
                     GKRPLMGAIAAKLADRAIVTDDNPRGEDPAAIRAEVLGGMDKSAVEIGDRRAAIFEAV
                     AGLKSGDVLVVAGKGHEQGQIVAGQVLPFDDVSVAREALGA"
     gene            complement(3166..>3690)
                     /locus_tag="VF402_07940"
     CDS             complement(3166..>3690)
                     /locus_tag="VF402_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7800265.1"
                     /translation="MSYAEAAGAVLNGGILRPLTIRKYDGTSPLGGVRVMSPTTTRTM
                     LDLMRDNVLKGTGTRANVPGLRVGGKTGTAQKPVNGRYTNARISSFAAVFPTDGPVDS
                     DRYLVLVMFDSPQGSPSSSMVKTGAYVAAPVAGHIIERIAPFLGVKRVVDTFSNPNWD
                     KAPVQADDVTGGDE"
BASE COUNT          560 a         1364 c         1203 g          563 t
ORIGIN      
        1 tgcccgtcgc gcgcgccctt cagcgccaga aacacatcgc ccggcgccag cgcgcggctg
       61 tcgaaatcaa ccccgttcgc ggacctggcc gcgccttcgt cgagtcccgt cagcgtgccg
      121 ccggtggcct gcgccagttc cgcaaaggtc caaaggggcc gctccgccat cgatatatcc
      181 tgctacgcgc gtgaaagcgc cattgaaccc gactcaaacg attaggcaag cccagtttcg
      241 ttaatagacc gggcaacgca tccagacata gggccgcgcc cattgcacgc ccatcgacct
      301 gacgatcacg aagccgcgcg attccgccgg gtcgtcgccg acggcggaac agttgcgcag
      361 ggcgacgccc ggcgtataga ccgtgtcgcg cttggccatg agcgcgccgt ccttgcccag
      421 gctgacgcgc gcgacgtcat agctgatata gccaccgtca tagagatggt cgtcgaggcg
      481 cgcctcctga ttcagcacca ccacgaaggc cgaactctgg cgcgtgcgtt gcagatagcc
      541 gcgcgcctga tcgaagcagc cgtccggcga tttttcatcg cggtcgcgcc agcggtgacg
      601 cgcgcaatcg acgatgaaca tgtcctgacc gtaggcctca tacgtctcgg tcggaaccgg
      661 cgccggcggc gcgccgcaac ggttcagcgc cttttcggtg atgtagaggc cgtcctcgcc
      721 cggctcgacg taccagcgga cggcctcgac cggcgcgcag ccgtccacgg ccgtcatcat
      781 cgaggtcttg aaccagcggc tgtcggcctt gaacttggcg tcgcccttgc gcttcagcac
      841 ccattgctgg atgtcgtcgt cgccgagaac gtccccggcg cgcgccgccg ccctggtcgc
      901 gatcaggtcc agctccttct cgtcgggatg cgattgcaga tagcgcacgg ccgtctcggc
      961 gcaccagtcg gtcttgacgc tgaaccgcgg atcgcacgac cccggttgcg ccaggtagcg
     1021 gcgctggccg gcgaccagcc gcatttcggt gacatgccaa ccgccgggac cggagcggat
     1081 cgggtcgccc tgcggcgcat aggggccgtg cggcgcgcgc gggccgccgc cgcaactgac
     1141 gatctggctc tccaccgcca ccagcccgcc gttgcggctg ccgatcccgt agccgacatc
     1201 ctcgccgtcg agcgcgaagc aatcgcgcgg cacgctcagg ccggaggtcg gcagcggcag
     1261 ggcctgcgcc tgcgcgcccc tggcgtcgcg ccagaccgta tagagcgtga aatcccggcc
     1321 ataggccccc gcgacgcgcg cgccgggctg cgccggacgc gccagcatca gcacgcgcga
     1381 cggcgtggcg tcgagcgcga tcccgtcgag gcaggggcca tccttgaggg gcgccagttt
     1441 gcaacccggc ttggccgcga ccagcggcac ggcgtcgccg gtgacttcgc gcacctcgcc
     1501 gaacaccgcc ggtttgatca gacgcggcgc cgcgtcctgc gcccgtgaca gggacggcgc
     1561 cgtcatgccc aacgctaata cgcccacagc gataaaacgc aacattccgc cctcccgaac
     1621 agcttttccc gaacagcttt ccctgagacg gcatagcccg gatcggccgc gatgttaacc
     1681 cttcacggga tcaggcgccc agcgcttcgc gcgccaccga gacgtcgtcg aacggcagaa
     1741 cctgcccggc gacgatctgg ccctgctcgt ggcccttgcc cgccaccacc agcacgtcgc
     1801 cgcttttcag cccggccacc gcctcgaaga tggcggcgcg gcggtcgccg atctcgacgg
     1861 cgctcttgtc catgccgccc agcacctcgg cgcggatggc ggcggggtct tcgccgcgcg
     1921 gattgtcgtc ggtgacgatg gcgcggtcgg ccagcttcgc ggcgatggcg cccatcagcg
     1981 gccgcttgcc ccggtcgcgg tcgccgccgc agccgaacac gcagaccagc ctgccgtgcg
     2041 tatgcgggcg cagcgccttc agcaccgtct ccagcccgtc cggcgtatgg gcgtaatcga
     2101 catagacctc ggcgccgttg cccttagcgc cgacgcgctc cagccgcccg cgcgcgccct
     2161 tgagccgccc cagggccgcg atcaccgccc cggcctcctc acccgccgcg atggccagcc
     2221 cggcggcgac cagcgcgttc gacgcctgaa acagtccggc cagcggcagc ttgacgtcgt
     2281 aggtccggcc ctgatgttcg agatagagga tctggccgtc cacggtcagg tcgcgcccgg
     2341 tcagggccag gccgcgcccg cgctcgccca ccgccatcac gctcaggccc gacaggatcg
     2401 acatggccgc gaacgcgtta tagccatcgg aatcggcgtt cagcacggcg gtccggccgc
     2461 gcggcagcaa ctgctcgaac aggcgcagct tggccgtgcg gtaggcgtcc atcgtcgcat
     2521 gatagtcgag atggtcctgc gtcaggttgg tgaagcccgc cgcgcacagc gacaccccgt
     2581 ccagccgccg ctgatcgatg ccgtgcgacg aggcttcgag cgcgacatgg gtgacgccgt
     2641 cttgcgccag ttcggccatg tggcgcgcca tgtcggcggc gtccggcgtg gtcaggcccg
     2701 gcggcgtgat cgccgtctcg gtcccgtccg ggttctggcg cacgacgccc agcgtgccca
     2761 tcgagccggt cgtgtggccg agcgccgcga agatctggcg gcagaaggtc gccacgctgg
     2821 tcttgccgtt ggtgccggtc accgccacgc aggttttcgg ctgcgcgccg taaaacgcct
     2881 tggcggccaa agcgtaggcg cggcgcacgt cggccacctg caccaccacc gactcgccgt
     2941 cctcgacgcc gaggcccttc ccgaacgggc cgtccggcgc caggatcgcc gccgcgccct
     3001 gacggatggc ctgcgggatg aagtcgcgcc cgtcggcctt cgcgcccggc agcgcgcaga
     3061 acagcgtccc gtccgccacc ttgcggctgt ccgccgtcac gccggtcacg acgggatcgc
     3121 ggtccagatc gcggcgcagg atgtcggaaa gccgcacggc gctcattact catcacctcc
     3181 ggtaacgtca tcggcctgaa cgggcgcctt gtcccagttc ggattgctga atgtgtccac
     3241 gacgcgcttc acgcccagga acggcgcgat gcgttcgatg atatggcccg cgaccggcgc
     3301 cgcgacatag gcgcccgtct tgaccatgct cgaactgggc gaaccttgcg gcgagtcgaa
     3361 catcaccagc accagatatc ggtcgctatc cacaggtccg tcggtcggaa agacggcggc
     3421 gaaggacgaa atgcgcgcat tggtgtagcg cccgttgacc ggcttctgcg ccgtgccggt
     3481 cttgcctccg acgcgcaggc ccggcacgtt ggcgcgcgtg cccgtgcctt tcagcacatt
     3541 gtcgcgcatc aggtccagca tggtgcgcgt ggtcgtcggc gacatgaccc tgacgccgcc
     3601 cagcggcgac gtcccgtcat atttgcggat ggtcagcggg cgcaaaatac cgccgttcag
     3661 caccgcgccc gccgcctcgg cataggacat
//
LOCUS       DASVLL010000477         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_353057,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000477 DASVLL010000000
VERSION     DASVLL010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353057"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF402_07945"
     CDS             <1..508
                     /locus_tag="VF402_07945"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX7800266.1"
                     /translation="IEDLRAIPWVFSWTQTRFMLPGWYGFAAAVRALKIDDETLRAML
                     EWDFFDVFLANMEMALASCDMELAKSYAGLARDAKEAARIYETIRFEFEDTVALILRI
                     RRTDALLSTQPRLRRAIDRAQPLLRSLNRLQVYLMAQRRHGNQHKLLKLALQLTVNGV
                     ASGLRNTG"
     gene            complement(528..1652)
                     /locus_tag="VF402_07950"
     CDS             complement(528..1652)
                     /locus_tag="VF402_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7800267.1"
                     /translation="MGPISRAGRNGPRLFQGGVRVTQNKASSQPPAGNAVSTINDVAR
                     LAGVSIKTVSRVMNNEPNVREETRAKVKEAAALLHYRPNLLARSLAGSRSFLIGLLYD
                     NPSSSYVIDTQEGVIRRSRLSGYHVLSEPQDSLAPDLGRAIASLLATIRLDGVILTPP
                     LCDMGQVLDAIEAAGVPYVRVTPTTFPGRSAYVDMDDVQAAYDMTRYLLDRGHRDIGF
                     VRGHPEHGSSHRRYEGYTRAMKEAGLTPRPNHIAQGFNSFASGQAAAKSLFAAADDLP
                     SAVFASNDDMAFGVMAYAQQHGVRVPDQVSVVGFDDNPGAMIIWPHLTTIRQPVADLA
                     YAAADILLSRADAGEGVALRAASRLLPFELVRRESDREAG"
     gene            complement(1761..2396)
                     /locus_tag="VF402_07955"
     CDS             complement(1761..2396)
                     /locus_tag="VF402_07955"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX7800268.1"
                     /translation="MSTHPDIEKFMTMGPVLPVMVIPSLDQALPLADALIAGGIRVLE
                     ITLRTDCALDAIRLIAKERPEAVVGAGTVLTPKDAEKAARAGAKFLVSPGLTKTLAHQ
                     DALPLLPGVATSSEVMRALEWGFTHLKFFPAVPAGGVPYLKGIGGPLPQAKFCPTGGV
                     SEKNAADFLALKNVLCVGGSWVAPEKAMAEGNWAEITRLSAEAVAAFGKKA"
BASE COUNT          413 a          896 c          918 g          431 t
ORIGIN      
        1 gatcgaggat ctgcgcgcga tcccgtgggt gttttcatgg acgcagacgc gcttcatgct
       61 gcccggctgg tacggcttcg ccgccgccgt gcgcgcgctg aagatcgacg acgagacgtt
      121 gcgcgccatg ctggaatggg acttcttcga cgtgtttctc gccaatatgg agatggcgct
      181 ggcctcgtgc gacatggagc tggccaaatc ctatgccggt ctggcgcgcg acgcgaagga
      241 ggccgcgcgc atctacgaga cgatccgctt cgagttcgag gacacggtcg cgctgatcct
      301 gcgcatccgc cggaccgacg ccctgttgtc gacccagccc cggctgcgcc gcgccatcga
      361 ccgcgcccag cctttgttgc gcagcctgaa ccgcttgcag gtctatctga tggcccagcg
      421 ccgccacggc aaccagcaca agctgctcaa gctggccctg caactgaccg tcaacggcgt
      481 cgcgtcgggc ctgcgcaata cggggtgagg ggggcggaaa tcccgcctca gccggcctcc
      541 cggtcggact cgcggcgcac caactcgaac ggcagcaggc gagacgcggc gcgcaaggcg
      601 acgccttcac cggcgtcggc gcgcgacagg aggatgtcgg cggcggcgta ggcgaggtcg
      661 gcgaccggct ggcggatggt ggtcaggtgt ggccagatga tcatggcgcc gggattgtcg
      721 tcgaagccga ccacggaaac ctgatcgggc acgcgcacgc cgtgctgctg ggcgtaggcc
      781 atgacgccga aggccatgtc gtcattgctg gcgaagacgg cgctgggcag gtcgtccgcc
      841 gccgcgaaca gggatttcgc cgccgcctga ccggaggcga agctgttgaa gccctgcgcg
      901 atatggttcg ggcgcggcgt caggcccgcc tccttcatgg cgcgcgtata gccttcgtag
      961 cggcggtgcg acgagccgtg ctccgggtgg ccgcgcacga agccgatgtc gcggtggccg
     1021 cggtccagca ggtagcgcgt catgtcgtag gccgcctgca cgtcgtccat gtcgacatag
     1081 gccgagcgcc ccggaaaggt ggtcggcgtc acgcgcacat agggcacgcc cgccgcctcg
     1141 atcgcatcca gcacctgccc catgtcgcac aggggcgggg tcaggatcac gccgtcgagc
     1201 cggatggtgg ccaaaaggct ggcgatggcg cggccgaggt ccggcgccag gctgtcctgc
     1261 ggttcggaca ggacgtgata gccgctcagg cggctgcggc ggatcacgcc ctcctgcgtg
     1321 tcgatcacgt agctggagga cgggttgtcg tacaaaagcc cgatcaggaa ggagcgcgat
     1381 ccggccagcg agcgcgccag caggttcggg cggtagtgca acagcgccgc cgcctccttg
     1441 accttggcgc gcgtctcctc gcgcacgttc ggctcgttgt tcatgacgcg cgacacggtc
     1501 ttgatcgaca cgcccgccag ccgcgccacg tcgttgatgg tggagacggc gttgccggcc
     1561 ggcggctggc ttgaggcctt gttctgcgtc acccggacac cgccctgaaa caggcgcgga
     1621 ccgttgcggc ccgcgcggga aattggtccc ataacctgcc ttgcgaatga cagctttgtc
     1681 aaccgcgatc cggtcgtgtt ttcaggggct taccggaacg cctgtcccgc cttgtccaga
     1741 ggaccaacgg cggggcgcgc tcaggccttt ttgccgaacg ccgcgaccgc ctcggcggac
     1801 aggcgggtga tctcggccca gttcccctcg gccatcgcct tttccggcgc gacccacgag
     1861 ccgccgacgc acaatacgtt cttcagcgcc aggaagtccg ccgcgttctt ttccgacacg
     1921 ccgccggtcg ggcagaactt cgcctgcggc agcgggccgc cgatgccctt caggtaaggc
     1981 acgccgcccg ccgggaccgc cggaaagaat ttcaggtgcg tgaagcccca ttccagcgcg
     2041 cgcatcacct cggacgaggt cgccacgccc ggcagcagcg gcagcgcgtc ctgatgcgcc
     2101 agcgtcttgg tcaggcccgg cgagacgagg aacttcgcgc ccgccctggc ggccttttcg
     2161 gcgtccttcg gcgtcagcac cgtgcccgcg ccgaccacgg cctccgggcg ctccttggcg
     2221 atcagccgga tggcgtcgag cgcgcagtcg gtgcgcagcg tgatctccag cacccggatg
     2281 ccgcccgcga tcagcgcgtc cgccagcggc agcgcctgat ccagcgacgg gatcaccatg
     2341 accggcagca cggggcccat ggtcatgaac ttttcgatat cgggatgggt gctcatagcg
     2401 gtgtcctcat aaattcgcct ccccgtttac gggaacgtgg cggacgggct ggccgctggc
     2461 cagaccgggc tgacggaggg gcatcggacg gacggggtcg ccccttccgt catttcggcc
     2521 cttgcggacc tcaatgccac cttccccata aatggggaag gcgaaatttc cccctcgtca
     2581 ggcgagaatc tgatgatcgg tcagcgcgtt ggccgagccg agcagagcgg cgtagggatg
     2641 gatgatgatg ctggacgg
//
LOCUS       DASVLL010000478         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_407006,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000478 DASVLL010000000
VERSION     DASVLL010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407006"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..912)
                     /locus_tag="VF402_07960"
     CDS             complement(37..912)
                     /locus_tag="VF402_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7800269.1"
                     /translation="MDAALGEASPLRPGDPGAPRTEDIELRLLLEAVYQKYHYDFRGY
                     AMASVKRRMTQARQHFQRETFSQLQDLVLHDDAMMGRLLPFLTVQVSEMFRDPSYFRA
                     IREKVAPHLRTYPSLKVWIAGCSTGEELYSFAILFREEGLENRTLFYATDINAEALEQ
                     AEAGVYSLDRIRQFTENHRLSGGKSSLSDYYTAAYGAASFDKSLRRNVVFSDHSLATD
                     AVFAETHMVSCRNVLIYFDRALQDRAVGLFKDSLTHKGFLGLGSKETLRFSSQADAFV
                     DFAREERIYQKRERD"
     gene            complement(897..>2196)
                     /locus_tag="VF402_07965"
     CDS             complement(897..>2196)
                     /locus_tag="VF402_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800270.1"
                     /translation="AAEAPKAKKAKTPKTLSGPRFHDDRENLAPGKRTLLIVEDDETF
                     ARILYDLAHEQGFQCVVAATAEAALDAVEPYQPAAVLLDIGLPDHSGLSVLDRLKRDV
                     RTRHIPVHVVSGNDYQHAALSLGAAGYMLKPVKREELIGAFRQLETRLSRSVGRILIV
                     EDDTVQRESVARLLKSGDVETVAVGTAAECLELLKSQTFDCMVLDLNLPDATGYSLLE
                     TLSQEDAYSFPPVIVYTGRDLSADDEQRLRRYSNSIIIKGAKSPERLLDEVTLFLHQV
                     VSELSSEQQSLLKKARNRDSMLEGRRILVVEDDVRNVYAVTNIFEPLGAIIEIARNGK
                     EALDALNKANAGLKKGERGPDSIDLVLMDVMMPEMDGLTATREIRKRPEWAKLPVIML
                     TAKAMKDDQERCLEAGANDYMAKPLDVEKLISLVRVWMPR"
BASE COUNT          359 a          720 c          727 g          390 t
ORIGIN      
        1 cccggcgcgc gcgcgccgcc ttcgggtccg ccgccatcag tcgcgctccc gcttctgata
       61 gatgcgctcc tcgcgcgcga agtcgacgaa ggcgtcggcc tgcgaggaaa agcgcaaggt
      121 ctccttggag ccgaggccga gaaagccctt gtgggtcagc gagtccttga acaggccgac
      181 ggcgcggtcc tgcaaggcgc ggtcgaaata gatcagcacg ttgcggcacg acaccatgtg
      241 cgtctcggcg aacaccgcgt cggtggccag actatgatcg gaaaacacca cgttgcggcg
      301 caacgacttg tcgaacgagg ccgcgccata ggccgccgta taatagtccg acagggacga
      361 tttgccgccc gacaggcggt ggttctcggt gaactggcgg atgcggtcca gactgtacac
      421 cccggcctcc gcctgctcca gcgcctcggc gttgatgtcg gtggcgtaga acagggtgcg
      481 gttctccagc ccctcctcgc ggaacaggat ggcgaaggaa taaagctcct cgcccgtcga
      541 acagcccgcg atccacacct tcagcgacgg ataggtgcgc aggtgcggcg cgaccttttc
      601 gcggatggcg cggaaatagg acggatcgcg gaacatctcg ctgacctgca cggtcaggaa
      661 gggcagaagc cgccccatca tggcgtcgtc gtgcagcacc agatcctgaa gctgcgaaaa
      721 ggtctcgcgc tggaaatgct ggcgcgcctg cgtcatgcgg cgcttgaccg aggccatggc
      781 gtagccgcga aaatcgtagt ggtacttctg atacaccgct tcgagcagca ggcgcagttc
      841 gatgtcctcg gtgcgcggcg cgcccggatc gcccggcctg agcggtgagg cttcgcctag
      901 cgcggcatcc atacgcgcac cagagagata agtttttcaa cgtcgagcgg cttggccata
      961 tagtcgttgg cgccggcttc gaggcagcgc tcctgatcgt ccttcatcgc cttggcggtc
     1021 agcatgatca ccggcagttt cgcccattcg ggacgtttgc ggatttcgcg cgtggcggtc
     1081 aggccgtcca tctccggcat catcacgtcc atcagcacca gatcgatgct gtccgggccg
     1141 cgctcgccct tcttgaggcc ggcattggcc ttgttcagcg cgtcgagcgc ctccttgccg
     1201 ttgcgggcga tctcgatgat cgcgcccagc ggctcgaaga tgttggtgac cgcatagacg
     1261 ttgcgcacgt cgtcctcgac cacgaggatg cgccgcccct ccagcatcga gtcgcggttg
     1321 cgcgccttct tgagcaggct ctgctgctcg ctcgacagtt ccgacaccac ctgatgcagg
     1381 aacagggtga cctcgtccag caggcgttcg ggcgacttgg cgcccttgat gatgatcgag
     1441 ttggaatagc ggcgcaggcg ctgctcgtcg tcggcgctca ggtcgcggcc ggtatagacg
     1501 atgaccggcg ggaaggaata ggcgtcctcc tgactcagcg tctccagcag cgaatagccg
     1561 gtggcgtcgg gcaggttcag gtccagcacc atgcagtcga aggtctgcga tttcagcagt
     1621 tcgagacatt ccgccgccgt gccgaccgcc accgtctcga cgtcgccgct cttgagcagc
     1681 cgcgccacgc tctcgcgctg caccgtgtcg tcctcgacga tcaggatgcg cccgaccgag
     1741 cgcgacagcc gcgtctccag ttgccggaag gcgccgatca gctcctcgcg cttgaccggc
     1801 ttgagcatgt agcccgccgc gcccagcgac agcgccgcgt gctgataatc gttgccggag
     1861 acgacatgca ccgggatgtg gcgcgtgcgc acgtcgcgtt tcagccggtc cagcaccgac
     1921 aggccggaat ggtcgggcag gccgatgtcg agcagcacgg cggcgggctg atacggctcg
     1981 accgcgtcga gcgcggcctc ggcggtggcg gcgacgacgc actggaagcc ctgctcgtgc
     2041 gccagatcgt acaggatgcg cgcgaaggtc tcgtcgtcct cgacgatcag cagggtgcgc
     2101 ttgcccggcg ccagattctc gcggtcgtcg tggaagcgcg gcccggacag ggttttgggc
     2161 gtcttggcct tcttcgcctt cggcgcctcg gccgcc
//
LOCUS       DASVLL010000479         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_192172,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000479 DASVLL010000000
VERSION     DASVLL010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192172"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VF402_07970"
     CDS             complement(<1..929)
                     /locus_tag="VF402_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800271.1"
                     /translation="MALLHWTPEQRARFSRENLLFRHDLDKSDLFSDEALIALIDRYP
                     RERLEVFSMGYDPANWGEWYFGRRGDLDGRALMEGVKAGRLWLNLRKVNHADADIAAL
                     CDSMFSEIRKEAGVATLKQDLGLLISSPDAHVFYHLDIPLVMLWQVRGVKRLYLYPPK
                     DSCVSDRHREGVALRESDEQLPYDPACDREALIHDLRPGEMMTWEQNAPHRIVNHGML
                     NVSMSIEFMTPAAVWKANVLYANGLLRRWFGLNPSMMRSPKFLEPFKVAFARLVKLAG
                     GYRGHKSPLRPSFSLDPARPGVPVFDPDVTPPKR"
     gene            complement(933..>1550)
                     /locus_tag="VF402_07975"
     CDS             complement(933..>1550)
                     /locus_tag="VF402_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7800272.1"
                     /translation="QAQARFDADVVFGPVKARLPRPDDAHDGYIEAFFSRFGPDESQL
                     LSGYYGCGNSLIRRACLPEDRPPFSVAHNQMGGEDDQLFHSLMTNGARIAWAADAFVY
                     EDVPSARARLSYTLLRAFAFGQGPSYTAAKTGKPFVCAAWMMQGLAQAALFGLWGGAL
                     ALVRHRGAARRLDKAARGAGKVLWFPPFKIGFYGAALLRKKPKGR"
BASE COUNT          276 a          518 c          507 g          249 t
ORIGIN      
        1 cgcttcggcg gcgtgacgtc ggggtcgaac accgggacgc cgggacgcgc gggatcgagc
       61 gagaagctgg gccgcagcgg actcttatgg ccgcgatagc ctccggccag tttgacgaga
      121 cgcgcgaagg cgaccttgaa cggctccaga aacttcggcg agcgcatcat cgacgggttc
      181 agcccgaacc agcgccgcaa caggccattg gcgtacaaga cattggcctt ccagacggcg
      241 gcgggcgtca tgaactcgat cgacatggag acgttgagca tgccgtgatt gacgatgcgg
      301 tgcggcgcgt tctgctccca cgtcatcatc tcgccgggcc tgaggtcgtg gatcagcgcc
      361 tcgcggtcac aggccggatc gtaaggcagt tgctcgtcgc tttcgcgcag cgccacgccc
      421 tcgcgatggc ggtcggacac gcaactgtct ttcggcggat agaggtagag ccgcttgacg
      481 ccgcgcacct gccacaacat gaccagagga atgtcgagat gataaaagac atgcgcgtcg
      541 ggcgaggaga tcaacaggcc gagatcctgc ttcaacgtgg cgacgcccgc ttccttcctg
      601 atttccgaaa acatggaatc gcacagggcg gcgatgtcgg cgtcggcgtg attgaccttg
      661 cgcaggttca gccacaggcg ccccgccttg acgccctcca tgagcgcgcg cccgtccaga
      721 tcgccgcgcc gtccgaaata ccattcgccc cagttggccg gatcgtagcc catgctgaac
      781 acttccagcc gctcgcgcgg atagcggtcg atcagcgcga tcagcgcttc gtcgctgaac
      841 agatcgctct tgtcgagatc gtggcggaac agcaggttct cgcgcgaaaa cctggccctc
      901 tgttcgggcg tccagtgcag cagagccatg agctatcggc ctttcggttt cttcctgagc
      961 agcgccgcgc cgtagaatcc gatcttgaac ggcgggaacc acaacacttt tccggcgccg
     1021 cgcgccgcct tgtcgagcct gcgcgccgcg ccgcgatgac ggaccagcgc cagcgcgccg
     1081 ccccacaggc cgaacagcgc ggcctgcgcc agcccctgca tcatccatgc cgcgcagacg
     1141 aacggctttc ccgtcttggc ggcggtgtag gacggcccct gcccgaaggc gaaggcgcgc
     1201 agcagcgtgt aggacagcct ggcgcgcgcg gacggcacgt cctcatagac gaaggcgtcc
     1261 gccgcccagg cgatgcgcgc gccgttggtc atcaggctgt ggaacaactg atcgtcctcg
     1321 ccgcccatct ggttgtgcgc gaccgagaac ggcgggcggt cctccggcag gcaggcgcgg
     1381 cgtatcaggc tgttgccgca gccgtaatag ccgctcagaa gctgcgattc atcgggaccg
     1441 aagcgcgaga agaaggcttc gatatagccg tcgtgcgcat cgtccggccg cggcaggcgc
     1501 gccttgaccg gcccgaacac cacgtcggcg tcgaagcgcg cctgcgcctg
//
LOCUS       DASVLL010000480         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_516736,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000480 DASVLL010000000
VERSION     DASVLL010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516736"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VF402_07980"
     CDS             complement(<1..341)
                     /locus_tag="VF402_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX7800273.1"
                     /translation="MVTIILKDAALVARLRKALLGWYDAHGRDLAWRRGARDPYRVWL
                     SEVMLQQTTVPHAAPYYEKFLRLWPTARDLAAADDARVMAEWAGLGYYARARRLLACA
                     RVVARDHGGAFP"
     gene            340..903
                     /locus_tag="VF402_07985"
     CDS             340..903
                     /locus_tag="VF402_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX7800274.1"
                     /translation="MKRSLPSLEDSVRILQTMRTKRAPKPRPPIVKQVQPFLKSLETK
                     FAGMDDGLGALKARWPEIVGASLARVSEPARVIRGPKTRPGGALEIRVMGAYAPLIQH
                     QSATLLDRINLYFGGRRIERLRIVQGPLTQGARPAQAAPPRPLTPQEELALQKQVADV
                     ADDKLRAQLLKLGRGVMRRRNAPPSPR"
     gene            1032..>1505
                     /locus_tag="VF402_07990"
     CDS             1032..>1505
                     /locus_tag="VF402_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7800275.1"
                     /translation="MTFSNLLPHLTARLAMFGALALMAAGATGCAKKADLAAGEMTLG
                     NPQAKVTLVEYASVACPICAHVNETIMPQVRAKYIDTGKVLYVYKPMMTGNPNVAAAG
                     HLLAQCVAKKNGHDKYFSVVDAIMRSQAAMGGEETGYANARPVLWSIAQSAGMTEA"
BASE COUNT          276 a          543 c          472 g          214 t
ORIGIN      
        1 ggaaacgccc cgccgtgatc gcgcgccacc acgcgcgcgc atgccagcaa ccgccgcgcc
       61 cgcgcgtaat agcccagccc cgcccattcc gccatcacgc gcgcatcgtc cgccgccgcc
      121 agatcgcgcg ccgtcggcca tagcctgagg aatttttcgt aatagggcgc ggcgtgcggc
      181 acggtggtct gctgcaacat cacctcggac agccagacgc ggtacggatc gcgcgcgccg
      241 cgccgccagg ccagatcgcg cccgtgcgcg tcgtaccagc cgagcagcgc cttgcgtaaa
      301 cgcgcgacaa gggccgcatc cttcagtata atcgtcacga tgaaacgctc gctgccctct
      361 ctggaagact cggtccgcat cctgcaaacg atgcgcacga aacgcgcgcc gaaaccgagg
      421 ccgcccatcg ttaagcaggt tcagccgttc ctgaaaagcc tcgaaacgaa attcgccggg
      481 atggacgacg gcctcggcgc gcttaaagct cgctggcccg agatcgtcgg cgcctcgctg
      541 gcccgcgtca gcgaaccggc gcgcgtcatt cgcggcccga agaccaggcc gggcggcgcg
      601 ctggaaatcc gcgtcatggg cgcctatgcg ccgctgatcc agcaccagtc ggcgaccctg
      661 ctcgaccgca tcaacctcta tttcggcgga cgcaggatcg agcgcctgcg catcgtgcag
      721 gggccgctga cgcagggcgc ccggcccgct caggccgcgc cgccgcgccc gctcacgcct
      781 caggaggaac tggccctgca aaagcaggtc gcggacgtgg cggacgacaa gctgcgcgcg
      841 caactgctga aactggggcg cggcgtgatg cggcggcgga acgcgccgcc ctccccgcgg
      901 tgagcccgcg ttggaactgg cgcgccgacg ccatatttcc agcatatttt gcgctttaaa
      961 aaacccgccg acaggctaaa agaacggtaa cggccctgag gccgattccg ccttcatcat
     1021 cggggagtcc catgacgttt tccaatcttc tgccgcatct gaccgcgcgt ctggccatgt
     1081 tcggcgcgct ggcgctcatg gccgcgggcg cgacgggatg cgcgaaaaag gccgacctgg
     1141 ccgcgggcga aatgacgctg ggcaatccgc aggccaaggt cacgctggtc gaatacgcct
     1201 cggtcgcctg cccgatctgc gcccatgtca acgagacgat catgccgcag gttcgcgcca
     1261 agtacatcga caccggcaag gtgctgtacg tctataagcc gatgatgacc ggcaacccca
     1321 acgtcgccgc cgccgggcac cttctggcgc aatgcgtcgc caaaaagaac ggccacgaca
     1381 agtatttcag cgtcgtcgac gccatcatgc gctcccaggc cgcgatgggc ggcgaggaga
     1441 ccggctacgc caacgcccgc ccggtgctgt ggagcatcgc ccagtccgcc ggcatgaccg
     1501 aggcc
//
LOCUS       DASVLL010000481         5978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_516746,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000481 DASVLL010000000
VERSION     DASVLL010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516746"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1333
                     /gene="coaBC"
                     /locus_tag="VF402_07995"
     CDS             140..1333
                     /gene="coaBC"
                     /locus_tag="VF402_07995"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7800276.1"
                     /translation="MAPPQRILLIVGGGIAAYKTLELIRLLKKAGHEVRVVLTKAAQA
                     FVTPLSAAALSGGKVYTDLFDLTDETEMGHIVLSRQADLVVVAPATADLMAKLAGGQA
                     NDLASTLLLATDKRVLMAPAMNVRMWRHPATQRNLAALRADGVLFTGPDEGEMACGEF
                     GPGRMSEPDAIFAAIVAALTEAPKPLAGRRVVMTAGPTFEPLDPVRGLTNRSSGKQGY
                     LVAQKLAEAGAQVTLVSGPVALPTPAGVTRIDVETALEMYDAVHAQLPCDAFVAVAAV
                     ADWRAQAVAAEKQKKTAEDTLSLTLVKNPDILESVSKHLEARPRLVIGFAAETQKLED
                     YAKAKLASKGCDAIVANDVSDGVFGTAQNQATLVDLDGYTPIEGTSKAQIADFLVGYI
                     RKRLA"
     gene            1421..1876
                     /gene="dut"
                     /locus_tag="VF402_08000"
     CDS             1421..1876
                     /gene="dut"
                     /locus_tag="VF402_08000"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7800277.1"
                     /translation="MTDRNTLRFKRWPGADALPLPAYATDGAAGFDFRAHLDAPVTLA
                     PGERRLISTGFAVAVPHGYELQIRPRSGLAAKFGISMPNTPGTVDSDYRGLVQVCLIN
                     HGAEPFEIAPGDRIAQGVLAAAPQWALIEVDELDETARGAGGFGSTGRA"
     gene            complement(1975..2721)
                     /gene="dapB"
                     /locus_tag="VF402_08005"
     CDS             complement(1975..2721)
                     /gene="dapB"
                     /locus_tag="VF402_08005"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7800278.1"
                     /translation="MSIRFAINGYRGRMGQAVARVLGERDLPIVARFEKGDSFDLSQS
                     DAVIDFSTPEASLELLEACAGEGLARPKPLVHVIGSTGFTQDQIDRIAPFADQVVIVR
                     SGNYSLGVNLLAGLVKQAAARLDAADWDIEIFEAHHKRKVDAPSGTALMLGEAAARGR
                     GVDLADAKVAARDGITGARRAGDIGFSVVRAGGIVGEHSAIIASEDEIITLSHSARDR
                     TLFARGALQAALWGVRQQPGIYDMQDVLGF"
     gene            2876..4471
                     /gene="murJ"
                     /locus_tag="VF402_08010"
     CDS             2876..4471
                     /gene="murJ"
                     /locus_tag="VF402_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7800279.1"
                     /translation="MPDTAPPESAPVKKSTGLVRNSLINAAFTLISRFAGFARDLVIA
                     AYMGASGNIMADAYATAQNFPNLFRRIFAEGAFSTAFVPSYSAMLERDGKEKADKMAH
                     DAMATLVFLTVAFTLLCELTMPWLMYVLKPGFAATPEKMKWAVIFTQITMPYLPCMAI
                     VAHLAGVLNARNRFILSAAAPILLNAVTLACVIPTRTPFQSALWASIGTLIAGVLQAS
                     LLLWGARKTGAHVSLSIVPVFSKDINALLLLAVPGALAAAATQLNVFVSQFFSSGVKG
                     ATMWMAIADRFYQLPLGLVGVAIGTALLPALSRAVHAQDHEHAQKTMDQAVVFALVFA
                     LPAAVALVAMPYFLVDAVYVRGEFLTFDAQWTARCLFHYGWGVPAFVLTRILNPAFFA
                     RKDTFGPMKFALVSVVTNIVVGVSMFHVIGVPGLAVGTSAAAWVNVLLMLVTLHRRGT
                     WTIHKAALGKALLVLLASLGMAAFCFAASHYRLRIQSVVGGKEIAVLLVSLVGVFVYI
                     GLLFTSGAVRPSELKAMLRRRRG"
     gene            4477..5481
                     /gene="trpS"
                     /locus_tag="VF402_08015"
     CDS             4477..5481
                     /gene="trpS"
                     /locus_tag="VF402_08015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7800280.1"
                     /translation="MTSKRILSGIQASGSLHLGNYLGALKKFADMQNDEAERFYFIAD
                     LHAITVWQDPEKLLAQTREIAACYLAAGVDPVRSAIFPQSAVAGHVELAWIFNCVARL
                     GWLDRMTQFKDKAGKDKERASVGLYTYPVLQAADILLYKATHVPVGEDQRQHLELTRD
                     VAKKFNHDFGVDYFPVPETLYQGPGARIMSLRDGMAKMSKSDPSDNARINLTDTADMI
                     AGKIKKAKSDADVLPEAPEGLEDRPEAKNLVGIYAALSGQSVTEVLAEFGGKGFGAFK
                     PALAEVVVDKMSPVSERLRALLKDPAEIDSALRIGVEKARAVAEPVVKDVKKIVGLWG
                     "
     gene            5526..>5978
                     /locus_tag="VF402_08020"
     CDS             5526..>5978
                     /locus_tag="VF402_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX7800281.1"
                     /translation="MRKLLIVAAAMLVLAGCDSRTETSKTVVSDGQVSATTKVTANAD
                     GVHSGLKFTDYAVRAALGINPNTAAYVTVANTGKTPDRLVSASCACAKTASLHTMTMH
                     GTMMEMGDAKDGFPIAPGQTIALKPGGDHIMLMGLTQRPQDGGFVDVTL"
BASE COUNT          975 a         2048 c         1969 g          986 t
ORIGIN      
        1 ggacggcccg gcgtcgcagc ccgccagcat cagcagcccg gccgccatca gcgcgcgcgt
       61 tcgcattgtt ccccccttca cggcttgctc cctcgtcccc acctatggca caagcgcggc
      121 aaaaggaaaa ggaaccggca tggcccctcc ccaacgcatc ctgcttatcg tcggcggcgg
      181 catcgcggcc tacaagacgc tggagctgat ccgcctgctc aagaaggcgg gccacgaggt
      241 ccgcgtcgtg ctgaccaagg ccgcgcaggc gttcgtcacg ccgctcagcg ccgccgccct
      301 gtccggcggc aaggtttaca ccgacctgtt cgacctgacc gacgagacgg agatgggcca
      361 tatcgtcctg tcgcgtcagg ccgacctcgt cgtcgtcgcg cccgccaccg ccgatttgat
      421 ggccaagctg gcgggcggtc aggcgaacga tctggcctcg accctgctgc tggccaccga
      481 caagcgcgtg ctgatggccc cggcgatgaa cgtgcgcatg tggcgccacc cggcgacgca
      541 gcgcaatctg gcggcgctgc gcgcggacgg cgtgctgttc accggaccgg acgagggcga
      601 aatggcctgc ggcgagttcg ggccgggccg catgagcgag ccggacgcca tcttcgcggc
      661 gatcgtggcg gcgctgaccg aagcgccgaa acccttggcg ggcaggcgcg tcgtcatgac
      721 cgccgggccg accttcgagc cgctggaccc cgtgcgcggc ctgaccaacc gttcgtccgg
      781 caagcagggc tatctggtcg cgcagaagct ggccgaggcg ggggcgcagg tgactctggt
      841 gtccgggccg gtggccctgc cgaccccggc gggcgtgacg cgcatcgacg tcgagacggc
      901 gcttgagatg tacgacgccg tgcatgcgca actgccgtgc gacgccttcg tcgccgtcgc
      961 ggcggtcgcc gactggcgcg cgcaggccgt cgcggcggag aagcagaaaa agaccgccga
     1021 ggacacgctc agcctcaccc tggtcaagaa tcccgacatt ctggaatcgg tgtccaagca
     1081 tctggaggcg cgcccgcgcc tcgtgatcgg cttcgcggcg gagacgcaga agctggagga
     1141 ctacgccaag gccaagctgg cctccaaggg ctgcgacgcc atcgtcgcca acgacgtcag
     1201 cgacggcgtg ttcggcacgg cgcaaaatca ggccaccctg gtcgatctgg acggctatac
     1261 gccgatcgag gggaccagca aggcgcagat cgccgacttc cttgtcggct atatccgcaa
     1321 gcggcttgcc tgaaaccggc gcggcgggct atggaccggg acgccccccc ctgagacgac
     1381 gcccccctga gacgacagac tcctgacagg atagaccgcc atgaccgacc gcaacacgct
     1441 ccgtttcaag cgctggcccg gcgccgacgc cctgcccctg cccgcctacg ccaccgacgg
     1501 ggcggcgggc ttcgacttcc gcgcccatct cgatgcgccg gtcacgctgg cgcccggcga
     1561 gcggcggctg atctccaccg gcttcgcggt cgccgtgccg cacggttacg agttgcagat
     1621 ccgcccgcgc tccgggctgg cggcgaagtt cggcatatcg atgccgaaca cgcccggcac
     1681 ggtcgacagc gattatcgcg gtctggtgca ggtctgcctg atcaatcacg gcgccgaacc
     1741 gttcgagatc gcgcccggcg accgcatcgc gcagggcgtg ctggccgccg ccccgcaatg
     1801 ggcgctcatc gaggtcgacg agctggacga aacggcgcgc ggcgcgggcg gcttcggctc
     1861 gaccgggcgg gcctagagcg ttttccgatc tgattgcatc agatcggcgc tctaacttta
     1921 tgcttttacg cgctttttaa tccgaaaagt gcgtcacgct tttcggaaag cgctctaaaa
     1981 cccaagcacg tcctgcatat cgtagattcc cggctgctgg cggacgcccc atagcgccgc
     2041 ctgtagcgcg ccgcgcgcga acagggtgcg gtcgcgcgcc gagtgcgaca gggtgatgat
     2101 ctcgtcctcc gaggcgatga tggccgaatg ttcgccgaca atgccgcccg cgcgcaccac
     2161 cgaaaagccg atgtcgcccg cccggcgcgc gccggtgatg ccgtcgcgcg cggcgacctt
     2221 cgcgtcggcg agatcgacgc cgcgcccccg cgccgccgct tcgcccagca tcaatgcggt
     2281 gccggacggc gcgtccacct tgcgcttgtg gtgcgcctcg aaaatctcga tgtcccagtc
     2341 cgccgcgtcc agccgcgccg ccgcctgctt caccaggccc gccagcaggt tgacgcccag
     2401 cgaatagttg ccggagcgca cgatgaccac ctgatcggcg aacggcgcga tgcggtcgat
     2461 ctggtcctgc gtgaagccgg tcgagccgat gacatgcacc aagggcttcg ggcgcgccag
     2521 cccttcgccc gcgcacgcct ccagcagctc cagcgaggcc tcgggcgtcg agaagtcgat
     2581 caccgcgtcg ctctggctca ggtcgaagct gtcgcccttc tcgaagcgcg cgacgatcgg
     2641 caggtcgcgt tcgcccagca cgcgcgccac cgcctgaccc atgcggcccc gatagccgtt
     2701 gatcgcgaaa cgtatggaca tgggcggcgc tcctgcaaca aaggccgtct ggataggacg
     2761 gggcgacggc ggcgtcaatg tggttcagga cggttcggaa ggcgttaacc catacgcgct
     2821 tgcgggaagg cggcggcggg cttaaacggg aggctcatcc attcgccgag ccccgatgcc
     2881 cgacaccgcc cctcccgaaa gcgcacccgt caaaaaatcc accggactgg tgcgcaattc
     2941 gctgatcaac gccgccttca cgctgatctc gcgcttcgcg ggcttcgcgc gcgatctcgt
     3001 catcgccgcc tatatgggcg cgtcgggcaa tatcatggcc gacgcctacg ccacggcgca
     3061 gaacttcccc aacctgttcc gccgcatctt cgccgagggc gcgttctcga ccgccttcgt
     3121 gccgtcctat tccgccatgc tggagcgcga cggcaaggaa aaagccgaca agatggcgca
     3181 cgacgccatg gccacgctgg tgttcctgac cgtcgccttc accctgctgt gcgaactcac
     3241 catgccgtgg ctgatgtatg tcctgaagcc cggcttcgcg gccacgccgg aaaagatgaa
     3301 gtgggcggtg atcttcaccc agatcaccat gccctacctg ccgtgcatgg ccatcgtcgc
     3361 gcatctggcc ggggtgctga acgcgcgcaa ccgcttcatc ctgtcggcgg cggcgccgat
     3421 cctgctcaac gcggtgactc tggcctgcgt gatcccgacc cgtacgccgt tccagtcggc
     3481 gctgtgggcc tcgatcggca cgctgatcgc gggcgtgttg caggcgtcgc tgctgttgtg
     3541 gggcgcgcgc aaaaccggcg cccatgtcag cctgtcgatc gttccggttt tctcgaagga
     3601 catcaacgcg ctgctgctgc tggccgtgcc gggcgcgctg gcggcggcgg cgacccagct
     3661 caacgtcttc gtgtcgcaat tcttctcgtc cggcgtgaag ggcgcgacca tgtggatggc
     3721 catcgccgac cgcttttacc agttgccgct gggcctcgtc ggggtcgcca tcggcacggc
     3781 gctgctgccc gccctgtcgc gcgccgtgca tgcgcaggac cacgaacatg cgcagaagac
     3841 gatggatcag gcggtggtgt tcgcgctggt cttcgccctg cccgccgccg tggcgctggt
     3901 cgccatgccg tattttctgg tcgacgcggt ctatgtgcgc ggcgaattcc tgaccttcga
     3961 cgcgcaatgg acggcgcgct gcctgttcca ctacggctgg ggcgtgcccg ccttcgtgct
     4021 gacgcggatt ttgaacccgg ccttcttcgc gcgcaaggac acgttcgggc cgatgaagtt
     4081 cgcgctcgtc tcggtcgtga ccaatatcgt cgtcggggtg tcgatgttcc acgtcatcgg
     4141 cgtgccgggg ctggcggtcg gcacctcggc ggcggcgtgg gtcaacgtgc tgctgatgct
     4201 cgtcaccctg cataggcgcg gtacatggac gattcacaag gccgctctgg gcaaggcgct
     4261 gctggtcctg ctggccagcc tcggcatggc ggctttctgt ttcgccgcct cgcattatcg
     4321 cctcaggatt caaagcgtcg tcggcggcaa ggaaatcgcc gtgctgctgg tttccctcgt
     4381 cggcgtcttc gtctatatcg ggctcctctt caccagcggg gccgtgcgcc cctccgaact
     4441 gaaggcgatg ctgcggcgtc gcagaggata actatagtga ccagcaaacg catcctgtcg
     4501 ggcattcagg cgtccggctc gctccacctc ggcaactatc tcggcgcgct caagaagttc
     4561 gccgacatgc aaaatgatga ggccgagcgc ttctatttca tcgccgacct gcacgcgatc
     4621 acggtctggc aagaccccga aaagctgctg gcgcagacgc gcgaaatcgc cgcctgctac
     4681 ctggcggcgg gcgtggaccc ggtcaggtcg gccatcttcc cgcagtcggc ggtggccggt
     4741 catgtcgagc tggcctggat tttcaactgc gtggcgcgcc tcggctggct ggaccgcatg
     4801 acccagttca aggacaaggc gggcaaggac aaggaacgcg ccagcgtcgg cctctacacc
     4861 tatccggtgc tgcaggccgc cgacatcctg ctgtacaagg cgacgcacgt gccggtcggc
     4921 gaggaccagc gccagcacct cgaactgacc cgcgacgtcg ccaagaagtt caaccacgac
     4981 ttcggcgtcg actatttccc cgtgccggaa accctgtacc aggggccggg cgcgcgcatc
     5041 atgtccttgc gcgacggcat ggccaagatg tcgaagtccg atccttcgga taatgcgcgc
     5101 atcaacctga ccgacacggc ggacatgatc gccggcaaga tcaagaaggc caagtcggac
     5161 gccgacgtgc tgcccgaagc gcccgaaggt ctggaggacc gcccggaagc gaagaacctc
     5221 gtcggcatct acgccgccct gtcggggcag tcggtgaccg aggttctggc cgaattcggc
     5281 ggcaagggct tcggcgcgtt caaaccggcc ctggccgagg tggtggtgga caaaatgtcc
     5341 cctgtgtcgg aacgtctgcg cgcgctactg aaggatccgg cggagatcga cagcgccttg
     5401 aggataggcg tcgaaaaggc gcgcgcggtc gccgagccgg tggtgaagga tgtgaaaaag
     5461 atcgtcgggc tctggggtta gtccttgggg ctgatccccg cccggcggca taagggaggt
     5521 ttgtcatgcg taagcttctg atcgtagcgg cggcgatgct ggttctggcg ggctgcgaca
     5581 gccgcaccga gacgtcgaaa acggtggtca gcgacggcca ggtcagcgcg acgaccaagg
     5641 tgaccgcgaa cgccgacggc gtgcattccg ggctgaaatt caccgattac gccgtgcgcg
     5701 ccgcgctggg gatcaatccg aacacggcgg cctatgtaac cgtcgccaac accggcaaga
     5761 cgccggaccg gctggtgtcg gcgtcctgcg cctgcgcgaa gacggcctcg ctgcacacca
     5821 tgaccatgca cgggaccatg atggagatgg gcgacgcgaa ggacggcttt cccatcgcgc
     5881 cgggccagac gattgcgctc aagccgggcg gcgatcacat catgctgatg ggcctgaccc
     5941 agcgcccgca ggacggcggc ttcgtcgacg tgacgctc
//
LOCUS       DASVLL010000482         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_245674,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000482 DASVLL010000000
VERSION     DASVLL010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245674"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VF402_08025"
     CDS             <1..1211
                     /locus_tag="VF402_08025"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HEX7800282.1"
                     /translation="DWSHPLGDYDKTNQQIQLGWDVQHNVSDNYNYQSLTLDHPVDPP
                     QSPRASSVTQFNDDQVLSAAYATYQRQFGPLSFQAGLRVENLHEKIVSDNPVQATSHG
                     WSRDSLDYTPNLFFMYKLDPDGKTTLKLLYSRKIQRPQGSQLDPQIVYSEDGLTARSG
                     NINLHSATTDKYDFDINHDGTLYDLQGQFYYNATNGNFEQVQQALPGQPNVILTTYEN
                     SGDKRNYGFSGSFSYHSADRKLRLNLNPNIGYTVTDYIDFSTHMLKRAQGPNSSLNGR
                     VMYKITDKDNLSLGVQYRGKNVDVQGYRTAQQSVNLSWMHQIIQNKFMLTANLSNVVM
                     GPMSKSIQDSPVAYGWSRSENPGATFMVSLRYMFGQPMQRGDFRGRPDGPPGGWRGRG
                     GPGPGGPGGF"
     gene            1318..>2437
                     /locus_tag="VF402_08030"
     CDS             1318..>2437
                     /locus_tag="VF402_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEX7800283.1"
                     /translation="MKRYVRAMAMGAVFLLACVTGAHAATDPLAPTGRWSAVTTGEAA
                     TPPMGWNSWNAFHTDVTEDKVLASAKAIVDSGLAAKGYRYINIDDGWWLKRRVSDGRM
                     IIRTSIFPSAAVGGPEETSFKPFTDKIHALGLKAGIYSDIGANNCSQAFGGPDTPNLP
                     EGTVAEREVGLYGHADQDIRLYFGEWGFDYIKVDACGIRAFGADNPRVKSGLYRELPP
                     LIDVAEIRRTNIPAVRGLYQQVADALRRTRPKGDYVFSICAWGSADVRAWAKDVGNLS
                     RTSDDITPSWTRMLANFDSAADRALYAHPGSWNDPDMLFIGHGDFDEHHLTEARSHFA
                     LWSMLSAPLIIGYDLRRAPPELMDILGNRAVIAVDQDPA"
BASE COUNT          472 a          800 c          757 g          408 t
ORIGIN      
        1 gcgactggtc gcatccgctc ggcgactacg acaagacgaa ccagcagatt caactgggct
       61 gggacgtgca gcacaatgtc agcgacaatt ataattacca gtcgctgacc ctcgatcatc
      121 cggtcgaccc gccgcaatcg ccgcgcgcct cgtcggtgac gcagttcaac gacgatcagg
      181 ttctgagcgc cgcctatgcg acctatcagc gccagttcgg cccgctgtcg tttcaggcgg
      241 gcctgcgcgt cgagaacctg catgagaaga tcgtctcgga caatccggtg caggcgacct
      301 cgcacggctg gtcgcgcgac tcgctcgact atacgcccaa cctcttcttc atgtacaagc
      361 tggacccgga cggcaagacc acgctgaagc tgctgtattc gcgcaagatt cagcgcccgc
      421 aaggatcgca gctcgatccg cagatcgtct atagcgagga cggcctgacc gcgcggtcgg
      481 gtaatatcaa cctgcactcg gccacgaccg acaagtacga tttcgacatc aatcacgacg
      541 gaaccctgta cgatttgcag ggccagttct actacaacgc caccaacggc aatttcgagc
      601 aggtgcagca ggcgctgccg ggccagccga acgtcatcct gaccacctac gagaacagcg
      661 gcgacaagcg caattacggc ttcagcggca gcttcagcta tcattcggcg gaccgcaagc
      721 tacgcctgaa cctcaatccg aacatcggct atacggtcac cgactatatc gacttcagca
      781 cgcacatgct caagcgcgcg caggggccga actccagcct gaacgggcgc gtgatgtaca
      841 agatcaccga caaggacaat ctgagcctcg gcgtgcagta ccgcggcaag aacgtcgatg
      901 tgcagggcta ccgcaccgcg cagcagtcgg tgaacctgtc gtggatgcac cagatcatcc
      961 agaacaagtt catgctgacc gccaacctgt ccaatgtcgt catggggccg atgtccaaga
     1021 gcatacagga cagcccggtc gcctatggct ggagccgcag cgaaaatccc ggcgcgacct
     1081 tcatggtcag cctgcgctac atgttcggcc agccgatgca gcgcggcgat ttccgcggcc
     1141 gtcccgacgg tccgccgggc ggctggcgcg ggcgcggcgg ccccggaccc ggcggaccgg
     1201 gcggtttctg atgcgtccgt tcccggcgcg cgcctgcgga aagcggttgc gcgccgggcg
     1261 ggcttgaggc taaatggcgt ccgatattta taatataata aatacagggg agacgtcatg
     1321 aagcgttatg tccgggcgat ggcgatgggc gccgtctttt tgctggcctg cgtcacgggc
     1381 gcccatgcgg ccacggaccc gctggcgccg acgggacgct ggagcgccgt gacgacgggc
     1441 gaggcggcca cgccgcccat gggctggaac tcctggaacg cctttcacac cgacgtcacc
     1501 gaggacaagg tgctggcctc ggccaaggcc atcgtcgaca gcggtctggc ggccaagggc
     1561 taccgctata tcaatattga cgacggctgg tggctgaagc gccgcgtcag cgacgggcgg
     1621 atgatcataa ggacgtccat cttcccgtcc gccgccgtgg gcgggcctga ggaaaccagc
     1681 ttcaagccgt tcacggacaa gattcacgcc ttgggtctga aggccggaat ctattcggac
     1741 atcggcgcca acaactgctc gcaggccttt ggcggtcccg acacgccgaa cctgcccgag
     1801 gggacggtgg ccgagcgcga ggtcgggctt tacggccacg ccgatcagga catccggctc
     1861 tatttcggcg aatggggctt cgactacatc aaggtcgacg cctgcggcat ccgcgccttc
     1921 ggggcggaca atccgcgcgt caaatcgggg ctgtatcgcg aactgccgcc gttgatcgac
     1981 gtcgcggaaa tccgccgcac gaatatcccg gcggtgcgcg ggctctatca gcaggtcgcc
     2041 gacgcgctgc gccgcacgcg gcccaagggc gattacgtgt tctcgatctg cgcctgggga
     2101 tcggccgatg tgcgcgcctg ggccaaggac gtcggcaatc tgtcgcgcac cagcgacgac
     2161 atcacgccga gctggacgcg catgctggcc aatttcgaca gcgcggccga ccgcgccctc
     2221 tacgcgcatc ccggctcgtg gaacgatccc gacatgctgt tcatcggcca cggcgatttc
     2281 gacgaacatc acctgaccga ggcgcggtcg cacttcgccc tgtggtcgat gctgtcggcg
     2341 ccgctgatca tcggctacga cctgaggcgc gcgccgcccg aactgatgga cattctcggt
     2401 aatcgcgccg tcatcgccgt cgatcaggat ccggcgg
//
LOCUS       DASVLL010000483         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_462297,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000483 DASVLL010000000
VERSION     DASVLL010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462297"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VF402_08035"
     CDS             complement(<1..194)
                     /locus_tag="VF402_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800284.1"
                     /translation="MTSALPFTASERHGAVSLGVLALAACLALSGTAADARTRARAPS
                     LRAQILAETQHMLDAQKAQF"
     gene            451..2406
                     /locus_tag="VF402_08040"
     CDS             451..2406
                     /locus_tag="VF402_08040"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HEX7800285.1"
                     /translation="MPDSHDFIATSTGGTVDCKTTVEEYILQQDWRVNANANTSYSNA
                     GLINNAAGKIIANYWLDQVYAPDEAMAHRNADIHLHDLDCLTGYCAGWSLRALLDEGF
                     NGVPGKVSSRPPRHFREALGQMANFLGILQSEWAGAQAFSSFDTYLAPYVFKDRLSFE
                     DIRKAIRGFVYNLNVPARWGQSPFTNITLDMTVPPDLKDNFPTAGGLHLFKSVDDPEL
                     LEIARSRGAAALEDLSYRHFEPEMQQIVIAYYEVMTEGDAHGQPFTFPIPTVNIMEDF
                     DWDGPMARALFENTAKIGSSYFQNFIGSQYMLDADGQKVPNPEAYAPHAVRSMCCRLQ
                     LDLRELQKRGNGLFGSAEMTGSIGVVTLNMARLGYRFRGRFDDLMRETGRLMDLAMSS
                     LEKKRTFVQTLYDRGLYPYTKRYLPHFRNHFSTIGVNGMNEMVRNFTGDAFDVSDRRG
                     IALCERILDAMRARLRRYQEETGHLYNLEATPAEGTTYRFAKEDARRFPDILQAGFDQ
                     NIYYTNSSQIPVGHTEDPFEALDLQDGLQGKYTGGTVLHLYMSERISSGEACKRLVRT
                     VIENYRLPYITITPVFSVCETHGYLAGEQEQCPACGQATLVWTRVMGYHRPVASFNLG
                     KRGEHRERRHFKEQPRAAARFACEAAG"
BASE COUNT          480 a          807 c          765 g          407 t
ORIGIN      
        1 tcgaactgcg ccttctgggc gtcgagcatg tgctgcgtct cggccaggat ctgcgcgcgc
       61 aggctcggcg ccctggcgcg ggttctggcg tcggccgccg tgccggaaag cgcgaggcag
      121 gccgccaggg ccaggacgcc gaggctgacg gcgccatgcc gctcgctggc tgtgaagggg
      181 agggcggagg tcatgatgtg tcccttatgt aaggcgacgc gcataacggc gcgcagggcc
      241 gtgcataacg cgcgcgggcg cttacgcggt tgataaccgt caaccggccg ggccaagtcg
      301 gtaagggctg aaaggcggcg ggcgcgcgcc gccggacacg gcgcggagaa cttcgggccg
      361 ccgcttgata agcgtcaaca gctctcccga taacacaaca tatagtggct ggaatcggac
      421 tttgaactac atatagcaca ggaggcgcat atgcccgaca gccatgactt catcgcgacc
      481 tcgaccggcg gcacggtcga ctgcaagacg accgtcgagg agtacatcct gcagcaggac
      541 tggcgcgtca acgcgaacgc caatacctct tacagcaatg cgggcctgat caacaacgcg
      601 gcgggcaaga tcatcgccaa ctactggctg gatcaggtct atgcgcccga cgaggcgatg
      661 gcgcaccgca acgccgacat ccacctgcac gatctggact gcctgacggg ctattgcgcg
      721 ggctggagcc tgcgcgccct gctggacgaa gggttcaacg gcgttcccgg caaggtgtcg
      781 agccgcccgc cacggcattt ccgcgaggct ttggggcaga tggccaactt cctcggcatt
      841 ctccagtcgg aatgggcggg cgcgcaggcc ttcagctcgt tcgacaccta tctggcgccc
      901 tatgtgttca aggatcgcct gagcttcgag gacatcagga aggccattcg cggctttgtc
      961 tataatctca acgttccggc gcgctggggg caaagcccgt tcaccaacat cacgctggac
     1021 atgaccgtgc cgccggacct gaaggacaac tttccgacgg cgggcggcct gcacctgttc
     1081 aaatccgtcg acgacccgga gcttctcgaa atcgcccggt cgcgcggcgc ggcggcgctg
     1141 gaagacctgt cctaccgcca tttcgagccg gaaatgcagc agatcgtcat cgcctattac
     1201 gaggtcatga cggaaggcga cgcgcacggc cagcccttca ccttccccat accgaccgtc
     1261 aacatcatgg aggatttcga ctgggacgga ccaatggcca gggccttgtt cgagaacacc
     1321 gccaagatcg gcagctccta cttccagaac ttcatcggca gccagtatat gctggacgcc
     1381 gacggccaga aggttcccaa ccccgaagcc tatgcgccgc acgccgtccg cagcatgtgc
     1441 tgccgcctgc aactggacct gcgcgagctt cagaagcgcg gcaacggcct gttcggctcg
     1501 gccgagatga ccggctcgat cggcgtggtc acgctgaaca tggcccgtct ggggtatcgg
     1561 ttccggggcc ggttcgacga cctgatgcgc gaaaccgggc ggctgatgga tctggccatg
     1621 tcgtcgctgg agaagaagcg gacattcgtc cagaccctgt acgaccgcgg cctctatccc
     1681 tacaccaaac gctacctgcc gcacttccgc aaccatttca gcaccatcgg cgtcaacggc
     1741 atgaacgaga tggtgcgcaa tttcaccggg gacgccttcg acgtcagcga ccggcgcggg
     1801 atcgcgctct gcgagcggat tctggacgcc atgcgcgcgc gtctgcgccg gtatcaggag
     1861 gagacgggcc acctctacaa tctggaagcc accccggccg aaggcacgac ctaccgcttc
     1921 gccaaggagg acgccagacg tttccccgac atccttcagg cgggcttcga tcagaacatc
     1981 tattacacca attcgagcca gatcccggtc ggccataccg aagacccctt cgaagcgctc
     2041 gacctgcagg acggcctgca aggcaaatac accggcggca cggtcctgca cctctatatg
     2101 agcgagcgca tctcctccgg cgaagcgtgc aagcgtctcg tcaggacggt catcgaaaat
     2161 taccggctgc cctacatcac gatcacgccg gtcttttcgg tttgcgagac gcacggctat
     2221 ctcgccggcg agcaagagca gtgtccggcc tgcggtcagg ccacgctggt ctggacccgc
     2281 gtgatgggct accaccgtcc ggtcgccagc ttcaacctcg gcaaacgcgg cgagcacagg
     2341 gaacggcgtc atttcaagga acagccgcgc gcggcggccc gtttcgcctg cgaagccgcc
     2401 ggataggcca tgtgggaaaa gctgccgata acggggctga cccccttcac gtttcagga
//
LOCUS       DASVLL010000484         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_31398, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000484 DASVLL010000000
VERSION     DASVLL010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31398"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VF402_08045"
     CDS             <1..235
                     /locus_tag="VF402_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7800286.1"
                     /translation="LEKGMGTKEAARAAGVFWKDENDLLRQAQAWRFDALDALAADLI
                     EADKLCKSTGMPDALISERLYLQIAGKAARAGL"
     gene            complement(575..1459)
                     /locus_tag="VF402_08050"
     CDS             complement(575..1459)
                     /locus_tag="VF402_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7800287.1"
                     /translation="MSEKQRGLGRGLSALLGDQGVAAAVSETPKTQSEAARGLEQPIE
                     LLVRNPDQPRRLFRDEEIEELAASIREKGVLQPILVRPAPGQAGSYQIIAGERRWRAA
                     QKAGLHTVPVIIRDLDDLDVLEIGIIENVQRADLNAIEESRAYRVLMERFGRTQDALA
                     QAVGKSRSHIANSLRLLNLPEAVQDHVLQGRLSAGHARALITAHDPEGLAQVVIDKGL
                     SVRQTEALARDGGKLETKSARPAARGGDADTLSLEQDLQEALGLKVRLDDRGGKGELR
                     LAYATLEQLDELCRKLMK"
     gene            complement(1487..2317)
                     /locus_tag="VF402_08055"
     CDS             complement(1487..2317)
                     /locus_tag="VF402_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7800288.1"
                     /translation="MPAVTERKARVLAVANQKGGVGKTTTAINLGTALAAIGKRVLLV
                     DLDPQGNASTGLGVPKAQRASSLYDVIVDRRPIGDTAVQTAVPGLWLLPSDPDLSGVE
                     IELGQAERRSYRLRDALDGLEDAYAGKKGGYDYILIDCPPSLNLLTVNAMSAADAVLV
                     PLQCEFFALEGLTQLMRTIDLVRGSLNPKLGLQGIVLTMFDRRNALSGHVERDVRSHF
                     GELVYTTVIPRNVRVSEAPSFGKPALIYDMKCSGSQAYIKLARELVARERARKKAEAA
                     "
BASE COUNT          422 a          818 c          763 g          467 t
ORIGIN      
        1 gctggaaaaa ggcatgggaa ccaaggaggc cgcgcgcgcc gccggggtct tctggaagga
       61 cgaaaacgac ctgttgcgtc aggcgcaggc ctggcgtttc gacgcgctcg acgccctcgc
      121 cgccgacctg atcgaagccg acaagctgtg caaatcgacc ggcatgcccg acgcgctgat
      181 ttcggaacgg ctctatctgc aaatcgcggg caaggcggcg cgggctgggt tgtaagcttt
      241 tcgccacgaa aagcgcgaaa aagtaggtcc acagatttac acagattaac gcagattctg
      301 gcggtgcgga tggaaggggt ggtgcgcctt ttccaatcta aggacgcgct ttgcgcgtcg
      361 ttgtttgtcg cttacccgaa agagcgtcgg ttaagccccg ccgctaaaaa tctgcgttaa
      421 tctgtggacc tactttttcg tggccttggc ggagccaagg catttcgcgc ttttcgtggc
      481 ccaaaaaccc cacctctctc acgcatttga ccgcatccga ctacgctctg ccgcgccaaa
      541 ttcgcgtgag acgccgttct ccgaatttcc gtaattattt cataagctta cgacacagct
      601 catcgagctg ttcgagcgtc gcataggcca ggcgcagctc acccttgccg ccgcggtcgt
      661 cgaggcgcac cttcaggccc agagcctcct gcaagtcctg ctccagcgac agggtgtcgg
      721 cgtcgccgcc gcgcgccgcc ggtctggcgg atttcgtctc cagcttgccg ccgtcgcgcg
      781 ccagcgcttc ggtctgacgc accgacaggc ccttgtcgat cacgacctgc gccagccctt
      841 ccgggtcgtg cgcggtgatc agggcgcgcg cgtgaccggc gctcaggcgg ccttgcagca
      901 catggtcctg caccgcttcg ggcaggttga ggagacgcaa actgttggcg atatggctgc
      961 ggctcttgcc gaccgcctgc gccagcgcgt cctgggtgcg gccgaaacgc tccatcagca
     1021 cgcgataggc gcgcgattct tcgatggcgt tgagatcggc gcgctgcacg ttctcgatga
     1081 tgccgatctc cagcacgtcg agatcgtcga ggtcgcggat aatgaccggc accgtgtgca
     1141 ggcccgcctt ctgcgccgcg cgccagcgcc gttcgccggc gatgatctgg tacgacccgg
     1201 cctgaccggg ggcgggccgg accagaatcg gctgcaacac gcccttctcg cggatcgacg
     1261 cggccagctc ctcgatctcc tcatcgcgga acaggcgacg cggctgatcc gggttgcgga
     1321 ccagcagctc gatcggctgc tccagcccgc gcgccgcttc gctttgcgtc ttcggcgttt
     1381 cgctcaccgc cgccgccacg ccctgatcgc ccagcagggc cgacagaccg cgacccagac
     1441 ctctttgttt ttccgacata atttcttttc cgtcccggtt cgggttttag gccgcttcgg
     1501 ccttcttgcg cgcgcgctca cgcgccacca gttcccgcgc cagcttgata taggcctggc
     1561 tgcccgaaca tttcatatcg tagatcaggg cgggcttgcc gaaggacggc gcctccgaca
     1621 cacgcacgtt acgcggtatg accgtggtgt aaaccagttc gccgaagtgc gagcgcacat
     1681 cgcgctcgac atggccggac agggcgttgc ggcggtcgaa catggtcagc acgatgcctt
     1741 gcaggcccag cttggggttc aggctgccgc gcaccaggtc gatggtgcgc atcagttgcg
     1801 tcagcccctc cagcgcgaaa aattcgcatt gcagcggcac cagcaccgcg tcggcggcgc
     1861 tcatggcgtt gacggtcaag aggttcagcg acggcgggca gtcgatcagg atatagtcgt
     1921 aaccgccctt cttgcccgca taggcgtcct ccagaccgtc gagcgcgtcg cgcaggcggt
     1981 aggaacggcg ctcggcctga cccagctcga tctcgacgcc ggacaggtcg ggatcggacg
     2041 gaagcagcca caggcccggc accgccgttt gaacagccgt atcaccaatc gggcgacggt
     2101 cgacaatgac atcatagagc gacgaggcgc gctgcgcctt gggcacgccg aggccggtcg
     2161 aggcgttgcc ctgcgggtcg agatcgacca gcagcacgcg cttgccgatg gccgccaggg
     2221 ccgtgccgag attgatcgcc gtcgtcgtct tgccgacgcc gcccttctgg ttggcgaccg
     2281 ccagaacgcg ggctttccgt tcggtcacag ccggaatttc gggagcatta tcgggcacgg
     2341 cgcagcctct cgatatggat gacgcgaccg cggggatcgc tggcggactc aaaaatttct
     2401 acgtcaaaac gccagttttt accagcttcc gccagttcac tttccacgtt ttcccccttt
     2461 aaaaaccacg
//
LOCUS       DASVLL010000485         3556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_192430,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000485 DASVLL010000000
VERSION     DASVLL010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192430"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VF402_08060"
     CDS             <1..907
                     /locus_tag="VF402_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7800289.1"
                     /translation="PEPPVSMTTRDALAAARAAVRASLEGAPERGNLLGGLKSGVSRR
                     QTPAAKTPAKGKGGILMNALKVSSLASALVVVGTTGYLAFKNTTPDNVKSGGPIAAAA
                     VEAPDAAADAQNQQALKTRYDLALKALDARAPGAVDTLKKVANQGYAPAQYRLSLLYN
                     GQDRLIPADKTEARVWTQRAAEGGVAKAMYNLGAMYYEGDGGAQDHSLAAMWFRKAAE
                     RGVKDSQYNLGVLYERGDGVPLNPSEAYKWLRIAGNGGDKIAAREADDLAAQLTDEQR
                     QRAESAVTGFTPITDGAPPLPGSEG"
     gene            992..2446
                     /locus_tag="VF402_08065"
     CDS             992..2446
                     /locus_tag="VF402_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7800290.1"
                     /translation="MDAQARPRPAAPDLAAVKPLLPWLVAVAFFMESLDTTILNTGVP
                     VIADALNVTALSMKAVLSSYTLALAVFIPISGWMADRFGTRRVFASAIGLFTLGSLLC
                     GLATNIHWLVAFRVLQGMGGAMMAPVGRLILVRTFDHRELVRAMSFVSIPTLIAPMLG
                     PLLGGLIVDYLHWRVIFFVNIPVGLAGMALVAMKLPNFRDPKPRPLDFLGLILFSSGV
                     ALLSYVLEVFGEHRLGVGGVLGLLAIAGLLLFGYGLHAARTTFPLLILGLFRIRTFAT
                     AVSGSFITRLGIGGAPFLLPLLYQVGFGLTPVQSGLLIMPQAIGSMASKFLLPRILDR
                     FGYAGVLRVNTLSVGVLLALFGFISARTPIWLIVVLALAYGALTSLQYTSMNTLIYAD
                     VPRKQTSGASTIASTLQQLSVSFGVATAGLITLFFLPADTDDRHLMIDGLHKAFWLLG
                     GFTLISTLIFSTLRKGDGEAAKSAPAAAAPSVKG"
     gene            complement(2537..3034)
                     /locus_tag="VF402_08070"
     CDS             complement(2537..3034)
                     /locus_tag="VF402_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEX7800291.1"
                     /translation="MANDAATAPAITLGNPAVVGLAGFGMTTLALQLHNLGYIGIAPV
                     LWLGFIFGGLGQFIAGFMEQKTGNNFGFCAFVGYGAFWLWLCAYIVGDSTGLALFKLA
                     DSDLAYMLAGWGLFTFGLWIASWRVSKAMWLTFLTLTLGFAGLALVKWGHTELLKPAA
                     YDLIV"
     gene            complement(3202..>3556)
                     /locus_tag="VF402_08075"
     CDS             complement(3202..>3556)
                     /locus_tag="VF402_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX7800292.1"
                     /translation="LLLDPGEFYILASKTNVEIPAREAAEMLPIDPGVGEFRVHYAGF
                     FDPGFGTEEAQGTGSKGVLEVRCHETPFLLEDGQTVARLVYEPLTDKPEKLYGQLGSN
                     YQKQGLKLSKHFQPW"
BASE COUNT          569 a         1217 c         1169 g          601 t
ORIGIN      
        1 gcctgagccg ccggtgtcga tgacgacgcg cgacgctctg gccgccgcgc gcgccgccgt
       61 gcgcgccagt ctggaaggcg cgccggagcg cggcaacctg ctgggcgggc tgaaatcggg
      121 cgtatcgcgc cgccagacgc ccgccgccaa gactccggcc aagggcaagg gcggcatctt
      181 gatgaacgcg ctcaaggtct cgtccctggc cagcgcgctg gtggtggtcg gcacgaccgg
      241 ctatctggcc ttcaagaaca cgacgccgga caacgtcaaa tcgggcggcc cgatcgccgc
      301 cgccgccgtc gaggcgcccg acgcggccgc cgacgcgcag aaccagcagg cgctcaagac
      361 gcgctacgat ctggcgctga aggcgctgga cgcgcgcgcg ccgggcgcgg tcgacacgct
      421 gaaaaaagtc gccaatcagg gctatgcgcc cgcgcaatac cgcctgtcgc tgctgtacaa
      481 cggtcaggac cggctgatcc ccgccgacaa gaccgaggcg cgcgtgtgga cccagcgcgc
      541 ggccgagggc ggtgtggcca aggccatgta taatctcggc gccatgtatt acgaaggcga
      601 cggcggcgcg caggatcatt ccctggccgc catgtggttc cgcaaggccg ccgagcgtgg
      661 cgtcaaggac agccagtaca atctcggcgt gctgtacgaa cgcggcgacg gcgtgccgct
      721 gaacccgtcc gaagcctata agtggctgcg catcgccggc aacggcggcg acaagatcgc
      781 cgcgcgcgaa gccgacgatc tggccgcgca actgaccgac gaacagcgcc agcgcgccga
      841 aagcgcggtc acgggcttca cgccgatcac cgacggcgcc ccgcccctgc ccggctcgga
      901 aggctgatcg cggcggcgaa gaggcgtgac cggcgcttgc gcgccggggg ccttgcgtcc
      961 atagttacgg acgcccgttc ggagttcgcc cttggacgct caagcccgcc ccagacccgc
     1021 cgcgcccgat cttgcggcgg tcaagccgct gctgccctgg ctggtggcgg tggcgttctt
     1081 catggaatcg ctcgacacca ccatcctgaa caccggcgtg ccggtgatcg ccgacgcgct
     1141 gaacgtcacg gcgctgagca tgaaggcggt gctgtccagc tatacgctgg ccctggccgt
     1201 gttcattccg atctccggct ggatggccga ccgcttcggc acgcggcgcg tcttcgcctc
     1261 ggccatcggc ctgttcacgc tcggctcgct gctgtgcggg ctggcgacca atattcactg
     1321 gctggtggcg ttccgcgtgc tgcaaggcat gggcggcgcg atgatggcgc cggtggggcg
     1381 gctgatcctg gtgcgcacct tcgaccaccg cgagctggtg cgcgccatga gcttcgtgtc
     1441 gattccgacc ctgatcgcgc cgatgctggg gccgttgctg ggcgggctga tcgtcgacta
     1501 cctgcactgg cgcgtgatct tcttcgtcaa tattccggtc gggctggcgg gcatggcgct
     1561 ggtggcgatg aagctgccca atttccgcga tccgaagccg cgtccgctcg acttcctcgg
     1621 cctgatcctg ttcagttccg gcgtcgcgct gctgtcctac gtgctggagg tgttcggcga
     1681 gcatcgcctc ggcgtcggcg gcgtgctggg cctgctggcc atcgccgggc tgctgctgtt
     1741 cggctacggg ctgcacgccg ccaggacgac ctttccgctg ctcatcctcg gcctgttccg
     1801 catccgcacc ttcgccacgg cggtgtcggg cagcttcatc acccggctgg gcatcggcgg
     1861 cgcgccgttc ctgctgccgc ttttgtatca ggtcggcttc ggcctgacgc cggtgcagtc
     1921 gggcctgctg atcatgccgc aggccatcgg ctcgatggcc agcaagttcc tgctgccgcg
     1981 cattctcgac cgcttcggct atgcgggcgt gctgcgcgtc aacaccctga gcgtgggcgt
     2041 gctgctggcc ctgttcggct tcatctccgc gcgcacgccg atctggctga tcgtggtgct
     2101 ggcgctggcc tacggcgcgt tgacctcgtt gcagtacacc agcatgaaca cgctgatcta
     2161 cgccgacgtg ccgcgcaagc agaccagcgg cgcctcgacc atcgccagca cgctgcaaca
     2221 gctttcggtg tcgttcggcg tggcgacggc cgggctgatc accctgttct tcctgcccgc
     2281 cgacaccgac gaccgccatc tgatgatcga cggcctgcac aaggcgttct ggctgctggg
     2341 cggcttcacc ctgatctcga ccctgatctt ctcgacgctc agaaaaggcg acggcgaggc
     2401 cgccaaaagc gcccccgccg ccgcggctcc gtccgtcaag ggctgatcac aggatcggcc
     2461 gtcccatcgg cagcacgtcg cggccgaaca cgctcttgaa gatgatcgag aacatcatgt
     2521 accacgcggt cagcgctcac acgatcaggt cgtaggcggc gggcttcaga agctcggtat
     2581 ggccccactt caccagagcc agccccgcga agcccagcgt cagggtcagg aaggtcagcc
     2641 acatcgcctt ggagacgcgc cacgaggcga tccacagccc gaaggtgaac agcccccagc
     2701 ccgccagcat gtaggccagg tcgctgtcgg ccagcttgaa cagcgccagc cccgtcgaat
     2761 cgccgacgat gtaggcgcac agccacagcc agaaggcgcc gtagccgacg aaggcgcaga
     2821 aaccgaaatt gttgccggtc ttctgctcca tgaagccggc gatgaactgg cccagcccgc
     2881 cgaagatgaa acccagccac agcaccggcg cgatgccgat atagccgaga ttatgcagtt
     2941 gcagcgccag tgtggtcatg ccgaacccgg ccagaccgac cacggcggga ttgcccaggg
     3001 tgatggcggg cgcagtcgca gcgtcgtttg ccatgtatgt gtttcctctt aacccgtttt
     3061 gttttgtgat gtaaccggtt tgcccccggc cgcggcaagg gaaacacaga ttacgcgcaa
     3121 ctgcaatcgc gttcgatcag agggggcgcg ttcgaccaga ggaggagaag gcgaaaggtc
     3181 ggcggatcga ccgccagcct gctaccacgg ctggaagtgc ttgctgagct tcagtccctg
     3241 cttctggtaa ttcgacccca gttgtccgta cagcttttcc ggcttgtcgg tcagcggctc
     3301 atagaccagc cgcgcgaccg tctggccgtc ctccagcagg aacggcgtct cgtggcagcg
     3361 cacttccagc acgcctttcg agccggtgcc ttgcgcctcc tcggtgccga agcccggatc
     3421 gaagaaaccg gcgtaatgca cgcggaactc gccgacgccg gggtcgatgg gcagcatctc
     3481 ggcggcttcg cgcgccggga tctcgacatt ggtcttcgag gccagaatgt agaattcgcc
     3541 cggatcgagc agcagt
//
LOCUS       DASVLL010000486         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_245980,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000486 DASVLL010000000
VERSION     DASVLL010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245980"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VF402_08080"
     CDS             complement(<1..1075)
                     /locus_tag="VF402_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX7800293.1"
                     /translation="MRAQVTLDDKYRQESGPVFMGGVQALVRLPMLIRDQDRRRGLNT
                     AGFISGYRGSPLGGYDTQLELAAGHLAAHDIVFQHAVNEDLAAAAVWGTQHVPLFPGA
                     KTDGVFGLWYGKSPGVDRSMDAIRHANFAGTSPLGGVLAIAGDDHASKSSTLPNQSEF
                     AFMDAEIPVLAPATLDEVIDFGVRGVSLSRYSGCWVALKVLSDQMDSSAVVHLDPSQW
                     RSVIPAFDMPPEFYTEGVSIRLHDSPEAKERRHRHIKLPAAQAFARANGFDRLVMDAP
                     RPRIGIAASGKAYLHVLDALRLLRISGAEARDLGLRLWKVGLVWPLDPAAARAFAGGL
                     QTVLVVEERRALIEPQLRDALYDL"
     gene            1236..1715
                     /locus_tag="VF402_08085"
     CDS             1236..1715
                     /locus_tag="VF402_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7800294.1"
                     /translation="MNRDKQGHILDDIDRRILRLLREDAHLTTQEIADRVGLSQTPCW
                     SRVKRLEESGVIAGYVALLDADKIGRPLSVIVEVNLDQHDDDKIEAFARHLAALPEVT
                     DAWLTTGEYDYAIIAAVEDTEDYENFLRKRLLKFPGVRHARSTFCLRRLKRAASPSV"
     gene            1795..2217
                     /locus_tag="VF402_08090"
     CDS             1795..2217
                     /locus_tag="VF402_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX7800295.1"
                     /translation="MSLRPLLPLLALIPLSACAALPWSGDARSGLGGDWRLSATPDGA
                     VADPAKYELSFQPQGRAAFKLDCNRANGIWEAKPRGAGGALTFGPLAMTRALCGAGSL
                     DTRIARDMSKVVFYTLQGGTLSLNLKDGGAYRWIRTAR"
     gene            complement(2218..2469)
                     /locus_tag="VF402_08095"
     CDS             complement(2218..2469)
                     /locus_tag="VF402_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX7800296.1"
                     /translation="MAIAQSDLQARIEAAFPGAEVTITDLAGDGDHYRARIVSAAFNG
                     LPRVRQHQMVNRALADLLSGPLHALALETVAPGTPETKD"
BASE COUNT          486 a          958 c          914 g          453 t
ORIGIN      
        1 gcaggtcata gagggcgtcg cgcagttgcg gctcgatcag ggcgcggcgc tcctcgacca
       61 ccagcacggt ttgcaggccg ccggcgaagg cgcgcgccgc cgccggatcg agcggccaca
      121 ccagcccgac cttccatagc ctgaggccca gatcgcgcgc ctcggcgcct gaaatccgca
      181 acaggcgcag ggcgtccagc acatgcaggt acgccttgcc gctggcggcg attccgatgc
      241 gcgggcgcgg cgcatccatg accagccggt cgaagccgtt ggcgcgcgcg aacgcctgcg
      301 cggcgggcag cttgatatgg cggtggcggc gctccttggc ctccgggctg tcgtgcagcc
      361 ggatcgagac gccctcggtg taaaattctg gcggcatgtc gaaagcgggt atgacgcttc
      421 gccactgcga cggatcgaga tgcacgaccg ccgaggaatc catctggtcg gacagcactt
      481 tcagcgcgac ccagcagccg gaatagcgcg acagcgacac cccgcgcacg ccgaagtcga
      541 tcacctcgtc caaagtcgcg ggcgccagca ccggaatctc ggcgtccatg aaggcgaatt
      601 ccgactggtt gggcagggtc gaggacttgc tggcgtggtc gtcgcccgcg atcgccagca
      661 cgccgcccag cgggctggtc ccggcgaaat tggcgtggcg gatcgcgtcc atcgaccggt
      721 cgacgcccgg cgacttgccg taccacaggc cgaacacgcc gtccgtcttc gcgccgggaa
      781 acagcgggac atgctgcgtg ccccatacgg cggcggcggc gaggtcttcg ttgacggcgt
      841 gctgaaaaac gatgtcgtgc gcggccagat gtccggcggc cagctcaagc tgggtgtcgt
      901 agccgcccag cggcgagccg cgatagccgg aaatgaatcc cgccgtgttc agcccccggc
      961 ggcggtcctg gtcgcggatc agcatcggga gacgcaccag ggcctgaacg ccgcccataa
     1021 agaccggccc ggactcctga cggtacttgt cgtccaatgt gacctgcgcg cgcatcgatg
     1081 gtccctgttg ctttatctcg ttgcataaga gactatgccc attgcggcgg aatgtgcttc
     1141 cgaagtgcgc gccgcaaacc ggtggtcacg aaatagtttt tctgcgacaa tggccggaaa
     1201 cagaataatc ttttgctgcg gagcaatatc gcgcaatgaa cagggataaa cagggccata
     1261 tactggatga cattgaccgc aggattctgc ggctgctgcg cgaggacgcc catctgacga
     1321 ctcaggagat cgccgaccgg gtcggcctgt cgcagacccc gtgctggtcg cgcgtcaagc
     1381 ggctggagga aagcggcgtg atcgcgggct atgtcgcgct tctggacgcc gacaagatcg
     1441 ggcggccctt gagcgtcatc gtcgaggtca atctcgacca gcacgacgac gacaagatcg
     1501 aggcgttcgc gcgccatctg gccgccctgc cggaggtcac cgacgcgtgg ctgaccacgg
     1561 gcgaatacga ctatgcgatc atcgcggcgg tggaggacac cgaggattac gaaaacttcc
     1621 tgcgcaagcg gctgctgaaa tttcccggcg tgcgccacgc gcgctcgacc ttctgcctgc
     1681 gccgcctgaa acgcgccgcc tcgcccagcg tgtgatcaca atcccccatt gcttttttac
     1741 gccgctaacg cgcaggtacc gcctgtcggc cttctcttct tcggaaagac aaccatgtcg
     1801 ctgcgccctc tcctgccgct gctcgccctg atccctttaa gcgcctgcgc cgccctgccg
     1861 tggagcggcg acgcgcgcag cggcctcggc ggcgactggc ggctgagcgc cacgcccgac
     1921 ggcgcggtcg cggacccggc gaaatacgag ctgagctttc agccgcaggg ccgggcggcg
     1981 ttcaagctgg actgcaaccg cgccaacggc atctgggagg ccaagccgcg gggcgcgggc
     2041 ggcgccctga ccttcgggcc gctggccatg acgcgcgccc tgtgcggcgc gggttcgctg
     2101 gacacgcgca tcgcgcgcga catgagcaag gtcgtattct atacgctgca aggcggcacg
     2161 ctgtcgctga accttaagga cggcggggcg tacaggtgga tcaggaccgc gcggtgatca
     2221 gtctttcgtc tccggcgtcc ccggcgccac cgtctccagc gccagcgcgt gcagcgggcc
     2281 gctcagcagg tcggccaggg cgcggttgac catctgatgc tggcgcacgc gcggcaggcc
     2341 gttaaacgcc gccgagacga tgcgcgcgcg gtaatgatcg ccgtctccgg ccagatcggt
     2401 aatcgtcacc tcggcgccgg gaaaagccgc ctcgatgcgg gcctgaaggt cgctttgcgc
     2461 aatcgccatg tgagaactcc tctgaacgcg cgtgtctaat cgtttccgtg cgcctattca
     2521 accgccacga tcacatgcga cgccttgatc agcgccaccg ccgtcacccg gcccgcaaag
     2581 gcgttccgcc gccggcgccc agatcggtca gaaatacaac gctttcgcca ttcgcagcca
     2641 cttcggcttc tgataaagga caggaccatg acacatcttc tccgccgcca tctcctgatc
     2701 gccgccgccc tggtcatgct gcccgccgcc gcccatgcgg cggacgtcag cgtggcggtc
     2761 gcagccaact tcaccgaacc ggccaaggac atcgccgccg cgttcgagaa a
//
LOCUS       DASVLL010000487         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_516988,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000487 DASVLL010000000
VERSION     DASVLL010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516988"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VF402_08100"
     CDS             <1..336
                     /locus_tag="VF402_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX7800297.1"
                     /translation="GLGGDWRLTATPDGAVADPAKYQLSFQPQGRAAFKLDCNRANGI
                     WEAKPHGAGGALTFGPLAMTRALCGAGSLDTRIARDMGKVESYTLQGGALSLNLKDGG
                     AYTWVRTAR"
     gene            complement(337..570)
                     /locus_tag="VF402_08105"
     CDS             complement(337..570)
                     /locus_tag="VF402_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX7800298.1"
                     /translation="MAIAQSDLQARIEAAFPGAEVTITDLAGDGDHYRARIVSDAFNG
                     LPRVRQHQMVNRALADLLSGPLHALALETVAKD"
     gene            complement(722..1516)
                     /locus_tag="VF402_08110"
     CDS             complement(722..1516)
                     /locus_tag="VF402_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX7800299.1"
                     /translation="MNDSLEALVILRRGNGTQVGASRLALLRAVAELGSITAAAKAVG
                     LSYKAAWEAAQALNNLSPRPLIIAQAGGRSGGTAQVSEAGLVLLRAYDQLEARMAGQV
                     ARLEADLAGAGLSGGDLIRSLGMRTSARNAFAGRVTTVTDGAVNAEVALDIGGQEIVA
                     VVTRESVEALGLAPGVTAVALIKSSFVLLAGGHDDLPVSARNRLKGVVAAVREGAVND
                     EVTLDLGGGKSVTAVVTCGSRAQLGLTPGSPAQALIKASHVIVAVE"
     gene            1750..3252
                     /locus_tag="VF402_08115"
     CDS             1750..3252
                     /locus_tag="VF402_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7800300.1"
                     /translation="MRILFAAAVSSAALLAAVPALAQTPDQAKMDALQAQVDALKAQL
                     SVLQSRIDGLKSPPPVAPAVAVPAPVPAPAAAAPAAPPVKVAMKNGTPSIATSDGAFS
                     IAVHGVFQLDAAAYDQSKRLGAAVTGRDLNSGTNFRRARIGLDGKLFSDFDYRILLDF
                     GGAGAENVGHFHEAWLQYNGLKAMKLRVGEFAPNVGMEDAGSANASPFLERSSAAEVA
                     RGLGGGDTRMGISAFGGNERLIWAVALTGNLASSLNTQGGFSPATYDEQLGMTARLAG
                     VPFEGKNWLIHTGVNYSAVINPADMGASAATRYPVQLRERPELTVDVTRLIDTGALNA
                     QSAAATGLELGAQYGPLFAQSEAFHYAVERLNPAAGVSNPDFSGWYVEGGWALTGERR
                     KYNAATQAFDGLTPAYNFDPKSGHWGAFEVVARYSTLDLDYYRDAALAADRVLGGQQN
                     VTSLGLDWQLNPAIRFVFQGQAVKVDRINAAGAQIGQKYNAFAIRSQFGF"
     gene            3318..>4011
                     /gene="modA"
                     /locus_tag="VF402_08120"
     CDS             3318..>4011
                     /gene="modA"
                     /locus_tag="VF402_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7800301.1"
                     /translation="MPAAAAPAVTWAADVSVAVAANFTEPAKEIAAAFEKKTGDHVAL
                     SFGASGVFLSQIEQGAPFEVFLSADSERPQKLEADGFAVAGTRFTYAQGALVLWSATP
                     GYVDARGAVLKRGAYQHLAIADPAAAPYGQAAVETLNSLGLYAAAAPKLVKGSSIGQT
                     YGFVKSGAAELGFLALSQVIHESGGSMWRVPAAAYKPILQDAVLLKPGANDPAARAWL
                     AFLKSPQARRIIA"
BASE COUNT          637 a         1457 c         1273 g          644 t
ORIGIN      
        1 ggcctcggcg gcgactggcg gctgaccgcc acgcccgacg gcgcggtcgc cgacccggcg
       61 aaataccaac tgagcttcca gccgcagggc cgggcggcgt tcaaactgga ttgcaaccgc
      121 gccaacggca tctgggaggc caagccgcac ggcgcgggcg gcgccctgac cttcgggccg
      181 ctggccatga cgcgcgccct gtgcggcgcg ggttcgctgg acacgcgcat cgcgcgcgac
      241 atgggcaagg tcgaatccta tacgctgcaa ggcggcgcgc tgtcgctgaa ccttaaggac
      301 ggcggggcgt acacatgggt caggacggcg cggtaatcag tccttcgcca ccgtctccag
      361 cgccagtgcg tgcagcgggc cgctcagcag gtcggccaaa gcgcggttga ccatctgatg
      421 ctggcgcacg cgcggcaggc cgttgaacgc gtccgagacg atgcgcgcgc ggtaatgatc
      481 gccgtctccg gccagatcgg taatcgtcac ctcggcgccg ggaaaggccg cctcgatgcg
      541 ggcctgaagg tcgctttgcg caatcgccat gtcggaactc ctctgaacgc gcgtgtctga
      601 agcgctcttc cttatccaaa aagtgggacc actttttggg gaaacgcttg tctgatcgct
      661 cttccttatc caaaaagtgg tgaccacttt ttggggaaac gctctaatcg tttccgtgcg
      721 tctattcaac cgccacaatg acgtgcgacg ccttgatcag cgcctgcgcc gggctgccgg
      781 gcgtcagccc cagttgcgcg cgcgaaccgc atgtcaccac cgccgtcacg ctcttgccgc
      841 cgccgaggtc gagcgtcacc tcgtcattga ccgcgccttc gcgcacggcg gcgaccacgc
      901 ctttgagccg gttgcgcgcc gacaccggca ggtcgtcatg cccgcccgcc agcagcacga
      961 agctcgactt gatcagcgcc accgccgtca cgccgggcgc caggcccagc gcctcgacgc
     1021 tttcgcgcgt gacgacggcg acgatctcct gcccgccgat atcgagcgcg acctcggcgt
     1081 tgacggcgcc gtcggtcacc gtcgtcaccc ggcccgcgaa ggcgttgcgc gcgctggtcc
     1141 tcatacccag actcctgatc agatccccgc ccgaaagccc ggcgccggcc agatcggcct
     1201 ccagccgcgc cacctgcccg gccatccgcg cctccagttg gtcgtaagcg cgcagcagga
     1261 ccagtcccgc ctcgctgacc tgcgccgttc cgccggaacg cccgcccgcc tgcgcgatga
     1321 tcaacggacg gggcgacagg ttgttgagcg cctgcgccgc ctcccacgcc gccttgtagc
     1381 tgaggccgac cgccttcgcc gccgccgtga tcgaccccag ctccgccact gcgcgcagca
     1441 gggccagccg cgaggcgccc acctgcgtcc cattgccgcg ccgcaagatc accagagctt
     1501 cgagcgagtc gttcatcctt gcagtgtaag gccgcgccgc cgtttatggc cagtgcattc
     1561 tgacccgccg gtccccatgt cttacgttat gtaggttgac gacataacga ctctgattta
     1621 ggcaacggat tgcggggcgc gtccaggggc ggacgcacga aagcccaccg ggccaaagac
     1681 ggcgcgggcg atatatccgg taatccggtc cagttcatcc ttctcattac tcagctttca
     1741 ggagtcgcca tgcgcattct gttcgccgcc gccgtatcga gcgcggccct tctcgccgcc
     1801 gtacctgcgt tggcgcaaac cccggatcag gcgaaaatgg acgccttgca ggcgcaggtc
     1861 gacgcgctca aggcgcagtt gagcgtcttg cagtcccgca tcgacgggct gaaatcgccg
     1921 ccgcccgtgg cgcccgccgt cgccgttccg gccccggttc cagccccggc cgctgccgcg
     1981 cccgccgcgc cgccggtcaa ggtcgcgatg aagaacggca cgccgtccat cgccacgtcc
     2041 gacggcgcct tttccatcgc ggtgcatggc gtctttcagc tcgacgccgc cgcttacgac
     2101 cagagcaagc gcctcggcgc cgccgtcacg ggccgcgacc tgaacagcgg cacgaatttc
     2161 cgccgcgcgc gcatcggcct cgacggcaag ctgttcagcg atttcgacta ccgcatcctg
     2221 ctcgatttcg gcggcgcggg cgccgagaat gtcggccact tccacgaagc ctggctgcaa
     2281 tataacggcc tcaaggccat gaagctgcgc gtcggcgaat tcgcgcccaa tgtcggcatg
     2341 gaggacgccg ggtccgccaa cgcctcgccg tttctggagc gttcctcggc ggcggaagtg
     2401 gcgcgcggcc tcggcggcgg cgacacgcgc atggggatca gcgcctttgg cggcaatgag
     2461 cggctgatct gggccgtggc cctgaccggc aatctggcct cgtcgctgaa cacgcagggc
     2521 ggtttcagcc cggcgaccta cgacgaacaa ctgggcatga cggcgcggct ggccggggtt
     2581 ccgttcgagg gtaaaaactg gctgatccat accggcgtca actattccgc cgtcatcaac
     2641 cccgccgaca tgggcgcgtc cgccgccacg cgctatccgg tgcagttgcg cgaacggccc
     2701 gaactgacgg tcgacgtcac gcgcctgatc gacaccggcg cactcaacgc gcaatccgcc
     2761 gccgcgaccg gcctcgagtt gggcgcgcag tacggtccgc tcttcgccca gagcgaggcc
     2821 ttccactacg ccgtagaacg cctcaacccc gccgcgggcg tcagcaatcc cgatttcagc
     2881 ggctggtacg tcgaaggcgg ctgggcgctc accggcgagc gccgcaagta caacgccgcg
     2941 acccaggcct tcgacggcct gacgcccgct tacaatttcg acccgaaaag cggccattgg
     3001 ggcgcgttcg aggtggtggc gcgctattcg acactcgatc tcgactatta ccgcgacgcg
     3061 gcgctggccg ccgaccgcgt gctgggcggg cagcagaacg tgacctcgct gggcctcgac
     3121 tggcagctca atccggcgat ccgcttcgtc tttcagggac aggcggtcaa ggtcgaccgc
     3181 atcaacgccg ccggcgccca gatcggtcaa aagtacaacg ctttcgccat tcgcagccag
     3241 ttcggcttct gataaaggac aggaccatga ctcacctttt ccgccgtcat cttctgatcg
     3301 ccgccgccct gttcgtcatg cccgccgccg ccgcgcccgc cgtcacttgg gcggcggacg
     3361 tcagcgtggc ggtcgcggcc aatttcaccg aaccggccaa ggagatcgcc gccgccttcg
     3421 agaaaaagac cggcgaccac gtcgccctga gcttcggcgc gtccggcgtc tttctgagcc
     3481 agatcgaaca gggcgccccg ttcgaggtct ttttgtccgc cgactcggag cgcccgcaaa
     3541 agctggaggc cgacggcttc gcggtggcgg ggacgcgctt tacctatgcg cagggcgcgc
     3601 tggtgctgtg gagcgcgacg ccgggctatg tcgacgcgag gggcgcggtg ctgaaacgcg
     3661 gcgcctatca gcatctggcc atcgccgatc ccgccgccgc cccctacggc caggccgccg
     3721 tcgagacgct gaacagcctc ggcctctacg ccgccgccgc gccgaagctc gtcaagggat
     3781 cgtccatcgg ccagacctac ggcttcgtca aaagcggcgc ggcggagctg ggcttcctgg
     3841 ccctgtcgca ggtgatccac gaaagcggcg gctcgatgtg gcgcgtcccc gccgccgcct
     3901 acaagccgat cctgcaagac gccgtgctgc tgaagcccgg cgcgaacgat ccggcggcca
     3961 gggcgtggct cgccttcctg aaatcgccgc aggcgcgccg gatcatcgcc t
//
LOCUS       DASVLL010000488         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_141195,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000488 DASVLL010000000
VERSION     DASVLL010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141195"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /gene="recO"
                     /locus_tag="VF402_08125"
     CDS             <1..509
                     /gene="recO"
                     /locus_tag="VF402_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7800302.1"
                     /translation="RLEALDATPDVLDDALALTGLQSACAMTRLALPEREAQPGVYHA
                     LSALLGLMDMPEVWPAVYVRYEAGLLEALGFGLDLSACAVSGGHDDLIYVSPKSGRAV
                     SRAAGAPYHDRLLALPPFLLSSQGGLGEGDIARGLELTGFFLERHVFHPHNQPLPEIR
                     RRLAEALR"
     gene            579..1037
                     /locus_tag="VF402_08130"
     CDS             579..1037
                     /locus_tag="VF402_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800303.1"
                     /translation="MLPYSEQQLIDANVNPATGLATDYLNLFNEAVMLFEMGLDMPDM
                     AEELVDWHPRGYIEHFEQSGFEMKDVVVAAYMFAPGDIREAFDATVARALSVFDSSVH
                     ILLSTHVMDEAARADLDGRLAEMKALVIEMDAHIHGRVEADLQSEVDALF"
     gene            1126..3348
                     /gene="parC"
                     /locus_tag="VF402_08135"
     CDS             1126..3348
                     /gene="parC"
                     /locus_tag="VF402_08135"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX7800304.1"
                     /translation="MSDVMIPAGGDDGGRILDEPMSEALSRRYLAYALSTIMHRALPD
                     VRDGMKPVHRRIMYAMGDMRLTPEAAARKCAKVVGEVMGTYHPHGDQAIYDALVRMAQ
                     DFAQRYQLVDGQGNFGNIDGDNPAAMRYTECKLTPAAMMLLDGIGENAVDFRATYDEQ
                     DEEPVVLPAGFPNLLANGAMGIAVGMATSIPPHNVTELIDACRKLLAKPSTTLDELLE
                     IIPGPDFPTGGVCVEAAANIRDAYRTGKGSIRTRARYEVEDLGRGAWRIVVREIPYQV
                     QKSRLIEQLAALIEAKKAPLLGDVRDESDEEVRLILEPKSRNIEPEVLMESLFKISDL
                     ETRFAVNMNVLDAGGTPRVMNLRECLTAFLDHRREVLQRMSRWRLERIEKRLHLLDGL
                     MIAFLNLDEVIRIIREDETPKQTLMARFSLSDVQADYILDTRLRQLARLEEMSLRAEH
                     DKLSKERDELLDLLGDEKKQWKRIDQQLVEARKKLMSARRTTFAEAPSGFEVAAIESY
                     LPKEPITVILSKRGWIRAAKGKVEDPSELKFKEGDELGFLVPGYTTDKLLIFTSDGRF
                     LTLGCDKLPSARGHGEPLRLMLDIDEGARIVDLFMLTPGAKRVMVSKSGYGFIMNEDD
                     AVANKRAGKQVLNVEGTEAFAALPVTGDHIMVLGENNKLLVYPVSELPEMARGKGVKL
                     QAYKDGGVKDIAVADLTRGAVWYDGAGKAREFKDVKDYIGRRASAGRIAPRGLRKLRV
                     "
     gene            complement(3354..>3552)
                     /locus_tag="VF402_08140"
     CDS             complement(3354..>3552)
                     /locus_tag="VF402_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800305.1"
                     /translation="RASQAQWTGMSLDECVITSIETYVIFEVCGYLKLVECGISRETA
                     AQLIKSTADAVSKYWNYNKGC"
BASE COUNT          653 a         1108 c         1198 g          593 t
ORIGIN      
        1 cgcggctgga ggcgcttgac gccacgcccg acgtgctgga cgacgcgctg gcgctgaccg
       61 gcctgcaaag cgcgtgcgcg atgacgcggc tggccctgcc ggagcgcgag gcgcagccgg
      121 gcgtctatca cgccctgtcg gcgctgctgg gcctgatgga catgccggag gtctggcccg
      181 ccgtctatgt gcgctatgag gccggattgc tggaggcgct gggcttcggg ctggacctga
      241 gcgcgtgcgc ggtcagcggc gggcatgacg acctgatcta tgtcagcccg aagtcggggc
      301 gggcggtcag ccgcgcggcg ggcgcgccct atcatgacag gctgctggcc ctgccgccgt
      361 tcctgctgtc cagtcagggc gggctgggcg agggcgacat cgccaggggg ctggaactga
      421 cggggttttt cctggagcgg catgtgtttc atccgcacaa ccagcccctg ccggagatac
      481 gccggcggct ggccgaggcc ctgcgctgac ggcgggcagg attttggtaa ggtttaccgt
      541 gtcataaccg cataaggaat aaggactgtc gcgattccat gctgccttac agcgaacagc
      601 aactgataga cgccaacgtc aatccggcca ccggactggc gaccgactat ctcaacctgt
      661 tcaacgaggc ggtcatgctg ttcgaaatgg gcctcgacat gccggacatg gccgaggagc
      721 tggtcgactg gcatccgcgc ggctatatcg agcatttcga gcagtcgggc ttcgagatga
      781 aggacgtggt ggtcgccgcc tacatgttcg cgccgggcga catccgcgaa gccttcgacg
      841 ccacggtggc gcgcgcgctc agcgtgttcg attcctcggt ccacatcctg ctcagcacgc
      901 acgtcatgga cgaggcggcg cgcgccgacc tcgacgggcg gctggccgag atgaaggcgc
      961 tggtcatcga gatggacgcg catatccacg gccgcgtcga ggccgacctg caaagcgaag
     1021 tggacgccct gttttaggcg ttttggccgc gaataaccgg tctgtccacg ccgcaaagcc
     1081 ttaaccgctc ttgttttcat tccgttcccg ttttagcgtc gcggcatgag cgatgtgatg
     1141 attcccgcgg gcggcgacga cggcggccgt attctggacg agccgatgag cgaggcgctg
     1201 tcgcgccgct atctggccta tgccctgtcg accatcatgc accgggcgct gcccgacgtg
     1261 cgcgacggca tgaagccggt gcatcgccgc atcatgtacg cgatgggcga catgcgcctg
     1321 acccccgaag ccgcggcgcg caagtgcgcc aaggtggtcg gggaggtgat ggggacgtac
     1381 cacccgcacg gcgaccaggc gatctacgac gcgctggtgc gcatggcgca ggacttcgcg
     1441 cagcgctatc agcttgtcga cgggcagggc aatttcggca atatcgacgg cgataacccc
     1501 gccgccatgc gttacaccga atgcaagctg accccggcgg cgatgatgct gctggacggc
     1561 atcggcgaga acgcggtcga tttccgcgcc acctacgacg agcaggacga ggagccggtg
     1621 gtgctgcccg ccgggttccc gaacctgctg gccaacggcg cgatgggcat cgcggtcggc
     1681 atggcgacct cgatcccgcc gcacaacgtc accgagctga tcgacgcctg ccgcaagctg
     1741 ctggccaaac cgtcgaccac gctggacgaa ctgctggaga tcattccggg gccggacttc
     1801 ccgaccggcg gggtctgcgt cgaggccgcc gccaatatcc gcgacgccta ccgcaccggc
     1861 aagggcagca tccgcacccg cgcgcgctac gaggtcgagg atctggggcg cggcgcctgg
     1921 cgcatcgtgg tgcgcgagat tccgtatcag gtgcagaagt cgcgcctgat cgagcaactg
     1981 gccgcactga tcgaggccaa gaaggcgccg ctgctgggcg acgtgcgcga cgaatcggac
     2041 gaggaggtgc gcctgatcct cgagccgaag tcgcgcaata tcgagcccga agtgctgatg
     2101 gagagcctgt tcaagatatc cgatctggag acgcgcttcg ccgtcaacat gaacgtgctg
     2161 gacgcgggcg gcacgccgcg cgtgatgaac ctgcgcgagt gcctgaccgc cttcctcgac
     2221 caccgccgcg aggtgttgca gcgcatgtcg cgctggcggc tggagcgcat cgagaagcgg
     2281 ctgcacctgc tcgacgggct gatgatcgcc ttcctcaatc tcgacgaggt gatccgcatc
     2341 atccgcgagg acgagacgcc gaaacagacc ctgatggcgc ggttttcgct gagcgacgtg
     2401 caggccgact acattctcga cacccgcctg cggcaactgg cgcggctgga ggagatgagc
     2461 ctgcgcgccg agcatgacaa gctgtcgaag gaacgcgacg aactgctcga cctgctgggc
     2521 gacgagaaga agcagtggaa gcgcatcgac cagcagttgg tcgaggcgcg caagaagctg
     2581 atgtcggcgc ggcgcaccac cttcgccgag gcgcccagcg gtttcgaggt cgccgcgatc
     2641 gaatcctacc tgcccaagga gccgatcacg gtcatcctgt cgaagcgcgg ctggatccgc
     2701 gccgccaagg gcaaggtcga ggatccgtcg gagctgaaat tcaaagaagg cgacgaactc
     2761 ggcttcctcg tgccgggcta tacgacggac aagctgctga tcttcacctc ggacgggcgt
     2821 ttcctgacgc tgggctgcga caagctgccg tcggcgcgcg gccatggcga gccgctgcgg
     2881 ctgatgctcg acatcgacga gggggccagg atcgtcgacc tgttcatgct gacgccgggc
     2941 gcgaagcgcg tcatggtgtc gaagtccggc tacggcttca tcatgaacga ggacgacgcg
     3001 gtcgccaaca agcgcgcggg caagcaggtg ctgaacgtcg aggggaccga ggcgttcgcc
     3061 gccctgccgg tcaccggcga ccacatcatg gtgctgggcg agaacaacaa gctgctggtc
     3121 tatccggtgt cggaactgcc ggaaatggcg cgcggcaagg gcgtgaaatt gcaggcctat
     3181 aaggacggcg gcgtcaagga catcgccgtc gccgacctaa cgcggggcgc cgtctggtac
     3241 gacggcgcgg gcaaggcgcg cgagttcaag gacgtgaagg actatatcgg ccgccgcgcc
     3301 tcggcgggcc gcatcgcgcc gcgcggcctg cgcaagctca gggtttaagg aattcaacaa
     3361 cccttattat aattccagta ctttgagacc gcatctgctg ttgacttgat aagttgtgcc
     3421 gcagtctctc tagaaattcc gcattctaca agctttaaat atccacagac ctcaaaaatg
     3481 acataagttt caatgcttgt aattacacat tcatcaagtg acatacctgt ccattgagcc
     3541 tgagacgcgc gt
//
LOCUS       DASVLL010000489         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_31975, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000489 DASVLL010000000
VERSION     DASVLL010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31975"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF402_08145"
     CDS             <1..475
                     /locus_tag="VF402_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7800306.1"
                     /translation="PGNIFSEGSVGVVSRSGTLTYEAVFQTTNAGLGQTTAVGIGGDP
                     VKGTEFIDVLEMFLADEATKSIIMIGEIGGSAEEDAAQFILDEARRGRKKPMAGFIAG
                     RTAPPGRRMGHAGAIISGGKGGAEDKIAAMEAAGIKVAPSPAALGETLLSVLKGL"
     gene            787..>2338
                     /locus_tag="VF402_08150"
     CDS             787..>2338
                     /locus_tag="VF402_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7800307.1"
                     /translation="MADDSSRLNQVFAETSFLYGTNAIFIEQMQEQWARDPNSVSPEW
                     RAFFDSLKDRPEAVNANADVAAWGRPDGAPRDELISAMDGVWPAVQAKVEKGLAAKTP
                     GASPADLQNAARDSIRALMLIRTYRIRGHLQSNLDPLELEPKGDNPELHPSYWGFTDA
                     DMNRTIFIDGVLGLESATLREILQIVRRTYCGNIGVQYMHIADPAEKAWVQERIEGRD
                     KEISFTREGKIAILKKLIEAEGFEKFCHRRFPGTKRFGLDGGEAMIPALEQIIKRGGA
                     LGVQDIIFGMAHRGRLNTLTAVMGKPYRAMFHEFQGGSSLPADIEGSSDVKYHMGASS
                     DREFDGNKVHLSLTANPSHLEIVNPVVLGKTRAKQAAYLKTREIETNPDRSMAMGLLI
                     HGDAAFAGQGVGMECFALMDLKGYRTGGTLHFVINNQIGFTTAPRFSRSSPYPSDVAL
                     MAQAPILHCNGDDPEAVVFAAKMATEFRQKFAKDVVIDMFCYRRFGHNEGDDPTFTQP
                     IMYARIKNH"
BASE COUNT          454 a          767 c          734 g          383 t
ORIGIN      
        1 gccgggcaat atcttctccg aaggctcggt cggcgtcgtt tcgcgctccg gcaccctgac
       61 ctacgaggcc gtgttccaga ccaccaatgc gggcctgggc cagaccacgg cggtcggcat
      121 cggcggcgac ccggtcaagg gcacggaatt catcgacgtg ctggagatgt tcctcgccga
      181 cgaagcgacc aagtcgatca tcatgatcgg cgaaatcggc ggctcggcgg aagaagacgc
      241 cgcccagttc atcctcgacg aagccaggcg cggccgtaaa aagccgatgg ccggtttcat
      301 tgcaggccgc acggcgcctc cgggccgccg catgggccat gccggcgcca tcatctccgg
      361 cggcaagggc ggcgcggaag acaagatcgc ggcgatggaa gccgccggga tcaaggtcgc
      421 gccttcgccc gcggccctcg gcgagacgct gctgagcgtc ctgaaaggtc tgtaagacga
      481 atttaccgca aggcgccaaa cttcattaag cagtttggcg ccttttgcgg taatatgcgt
      541 aaattcgtcg cacccgcccc aaaacggtca tcgccgtggg gtagggacgg cgctccaccg
      601 gacgggcggc gatgttcagc caaagccgca ggtccatatt ttcaaagcct gccccgtggt
      661 gtcgtgattg gctgcgggtt caacggcgct caagcctcag tccgtcatag ggcctagggg
      721 cggttgcgca aaaccttatc tgaaagggag gcggtgtcgt cattggccgc ctcgacgggt
      781 agcaatatgg cggatgattc cagccggctg aaccaggtgt tcgcggagac ttcgtttctc
      841 tacggtacga acgctatttt catcgaacag atgcaggaac aatgggcgcg cgatcccaat
      901 tcggtttccc ccgaatggcg cgcgtttttc gattccctga aggaccgccc cgaagcggtc
      961 aacgccaatg cggacgtggc cgcatggggc cgccccgacg gcgcgccgcg cgacgagctg
     1021 atctcggcga tggacggcgt gtggcccgcg gtgcaggcca aggtcgaaaa gggactcgca
     1081 gccaagacgc ccggcgcttc gccggcggac ctgcaaaacg ccgcgcgcga ttcgatccgc
     1141 gccctgatgc tgatccgcac ctatcgtatt cgcggccacc tgcaatccaa tctcgatccg
     1201 ctggagcttg agcccaaggg cgacaatccg gagctgcacc ccagctactg gggcttcacc
     1261 gatgcggaca tgaaccgcac catcttcatc gacggcgtgc tgggtctgga atcggcgacg
     1321 ttgcgcgaga tcctgcaaat cgtgcgccgc acctattgcg gcaatatcgg cgtgcagtac
     1381 atgcacatcg ccgacccggc ggaaaaggcc tgggtgcagg agcgcatcga gggccgcgac
     1441 aaggaaatct ccttcaccag ggaaggcaag atcgccatcc tgaagaagct gatcgaggcc
     1501 gagggcttcg agaagttctg ccaccgccgc ttccccggca ccaagcgctt cggcctcgac
     1561 ggcggcgaag ccatgatccc cgcgctggag cagatcatca agcgcggcgg cgcgctgggc
     1621 gtgcaggaca tcatcttcgg catggcccac cgcggccgcc tgaacacgct gaccgccgtg
     1681 atgggcaagc cctatcgcgc catgttccac gaattccagg gcggctcgtc cctgccggcg
     1741 gacatcgaag gctcgtcgga cgtgaagtac catatgggcg cctcgtccga ccgggaattc
     1801 gacggcaaca aggtgcacct cagcctgacc gccaacccgt cgcaccttga gatcgtcaac
     1861 ccggtcgtgc tgggcaagac gcgcgccaag caggccgcct atctgaaaac gcgcgagatc
     1921 gagaccaatc ccgaccgcag catggcgatg ggcctgctga tccacggcga cgccgccttc
     1981 gcgggtcaag gcgtcggcat ggaatgcttc gcgctgatgg acctgaaagg ctaccgcacc
     2041 ggcggcacgc tgcacttcgt gatcaacaac cagatcggct tcaccacggc gccccggttc
     2101 tcgcgctcgt cgccctatcc gtcggatgtg gcgctgatgg cgcaggcgcc gatcctgcac
     2161 tgcaacggcg acgatcccga agcggtggtg ttcgcggcca agatggcgac cgagttccgc
     2221 cagaagttcg ccaaggacgt ggtcatcgac atgttctgct atcgccgctt cggccacaac
     2281 gaaggcgacg acccgacctt cacccagccg atcatgtacg cccggatcaa gaaccacc
//
LOCUS       DASVLL010000490         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_141616,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000490 DASVLL010000000
VERSION     DASVLL010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141616"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /gene="folK"
                     /locus_tag="VF402_08155"
     CDS             <1..493
                     /gene="folK"
                     /locus_tag="VF402_08155"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7800308.1"
                     /translation="GTIIIAYGANLTAPGVTTSQAFAAVVKALAENGVRVARASRLWR
                     SQAWPNPDDPPYVNAVMIVETDAPPAALLETLHRIEHDFGRRRGAGARNAPRTLDLDL
                     IAYGEKVRATTPVLPHPRAHERGFVMGPLSEIAPDWTHPALGRTAAELYAAVTVGRDA
                     QPV"
     gene            complement(481..1449)
                     /locus_tag="VF402_08160"
     CDS             complement(481..1449)
                     /locus_tag="VF402_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7800309.1"
                     /translation="MPKPPHLSHNWTGYLLAFLGSFLFATKGIWIKLAYRYGIDASGL
                     LSLRLGIAMPFYIGIGLMTWRRAKAAGRSDLPPVLKRPGLYLQAMLVGALGYFVASYT
                     DFVSLKSLSPQFERLILFTYPLFVILFGAAFFAQPLRLKSIWSFAIAYAGLALVFLTD
                     LKTSGPVVAVGALWCCASSVSFALYLLLAKPMIRRLGPSLFTSWAMCGAGVATLTQFF
                     ILHPRGLGVPLSASLLWLSLGLALTATVAPSYLISFALSRISSQANAVIGFVNPVFTL
                     IMSVAILAIPVTASDIGGTALVLLGVGLYIWLDQKAARQTPADQTG"
     gene            1682..2125
                     /locus_tag="VF402_08165"
     CDS             1682..2125
                     /locus_tag="VF402_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX7800310.1"
                     /translation="MKAQTLNAKTLNAKTLSGRAYAIDGDTIVIGGRHIRLFGVDAFE
                     HDQTCGRFQCGQAATRVMRARLQGQVVACSQQDVDPYGRAVAVCTAGGEDLGRVMVRA
                     GLAVAYRHFSLRYLPDETWAQAHRQGAWAYGFDSPQYWRRTHVRK"
     gene            complement(2186..>2677)
                     /locus_tag="VF402_08170"
     CDS             complement(2186..>2677)
                     /locus_tag="VF402_08170"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX7800311.1"
                     /translation="WKGRIVSLTWRSGVGFVWSQATLKPLRTFHYRGEGWGLTADAHN
                     LIMSDGTATIKFLDPVTLKTVRTITVTLDGQPLDQINELEWVKGELIANVWRTRQLAR
                     IDPKTGVVTGVVDLYNLPEIASPPADPDAVANGIAYDAAHDRLFLTGKLWPHLYEVRF
                     APE"
BASE COUNT          453 a          793 c         1018 g          413 t
ORIGIN      
        1 gggaacgata atcatagcct acggcgccaa tctgacggcg ccgggcgtga cgacttcgca
       61 ggctttcgcg gcggttgtca aggcgctggc ggaaaatggc gtgcgcgtgg cgcgggcgtc
      121 gcggttatgg cgctcgcagg cgtggccgaa cccggacgat ccgccctatg tcaacgccgt
      181 catgatcgtc gagacggatg cgccgcccgc cgctctgctg gagacgctgc accggatcga
      241 gcacgatttc gggcggcggc gcggcgcggg ggcgcgcaac gcgccgcgca cgctcgatct
      301 cgacctgatc gcctatggcg agaaggtgcg cgcgacgacg ccggttctgc cgcatccgcg
      361 cgcgcatgag cgcggtttcg tcatggggcc gctgagcgag atcgcgccgg actggacgca
      421 tccggcgctg gggcggacgg cggcggagct gtacgcggcc gtgacggtgg ggcgggacgc
      481 tcagcccgtc tgatcggcgg gcgtttggcg ggcggccttc tggtccagcc agatgtagag
      541 gccgacgccg agcaggacca gcgccgtgcc gccgatgtcg gacgcggtga ccgggatggc
      601 caggatggcg acgctcatga tcagggtgaa gaccgggttg acgaagccga tcaccgcatt
      661 ggcctgtgac gagatgcggc tgagcgcgaa gctgatcagg tagctgggcg cgacggtggc
      721 ggtcagggcc aggccgaggc tgagccacag cagggatgcg gacagcggga cgccgaggcc
      781 gcgcggatgc aggatgaaga actgcgtcag ggtcgcgacg cccgcgccgc acatcgccca
      841 cgaggtgaac agggacggcc ccagccgccg gatcatcggc ttggccagca gcaggtagag
      901 cgcgaagctg accgaggagg cgcagcacca cagggcgccg accgcgacga ccgggccgct
      961 cgttttcagg tcggtgagga acaccagagc cagcccggca taggcgatgg cgaagctcca
     1021 gatcgatttc agccgcaacg gctgggcgaa gaaggcggcg ccgaacagga tgacgaatag
     1081 cggataggtg aacaggatca gccgctcgaa ctgcggcgac agcgatttca ggctgacgaa
     1141 atcggtgtag gaggcgacga aatagccgag cgcgccgacc agcatggcct gcaggtacag
     1201 gccgggacgc ttcaggaccg gcggcaggtc ggaccggccc gccgccttcg cgcgccgcca
     1261 ggtcatgagg ccgatgccga tgtaaaacgg catggcgatg ccgaggcgca aggacaggag
     1321 gccgctggcg tcgatgccgt agcggtaggc cagcttgatc cagatgccct tggtggcgaa
     1381 caggaacgag ccgaggaagg ccagcagata gccggtccag ttatggctca gatgaggcgg
     1441 cttcggcatg gacgggtccg gtgaccgggc attgacggcg ggcgcggatc aggctttctg
     1501 ggcgagcagg cgtttggccg cccagatcag gttggacagg atgtcgagac gtgcgagcgc
     1561 gaagctgttg gcgaaattcg tgaacatgac gggcgggggt gtacgccttc gcgggcgcgg
     1621 gatcaacctg tggatggcgt caacttgcgt taccgttctc gcgtgcatgg cgcccgccgg
     1681 cgtgaaggcc cagaccctga acgcgaagac cctgaacgcg aagactctaa gcggcagggc
     1741 ctatgcgatc gacggcgaca ccatcgtcat cggcgggcgg catatccggc tgttcggcgt
     1801 cgacgccttc gagcatgacc agacctgcgg gcggttccag tgcggtcagg cggcgacgcg
     1861 ggtgatgcgc gcgcggctgc aagggcaggt ggtggcctgt tcgcagcagg atgtcgatcc
     1921 ttacggccgg gcggtggcgg tctgcacggc gggcggcgag gatctgggcc gggtcatggt
     1981 gcgcgccggg ctggccgtgg cctatcggca tttctcgctg cgctacctgc cggacgagac
     2041 atgggcgcag gcgcatcgtc agggcgcctg ggcctacggc ttcgacagcc cgcaatactg
     2101 gcgccggacg catgtgcgga aatagggtcg gccacgaggc tccgggtttt cgtgtccctt
     2161 cgtgtttttc gtggcccgaa atcaactatt ccggcgcgaa ccgcacctcg tacaggtggg
     2221 gccagagctt gccggtgagg aacaggcggt cgtgggcggc gtcgtaggct atgccgttgg
     2281 cgacggcgtc gggatcggcg ggggggctgg cgatttcggg caggttgtag aggtcgacga
     2341 cgccggtgac gacgccggtt ttcgggtcga tgcgcgccag ttgccgcgtg cgccagacat
     2401 tggcgatcag ttcgcccttc acccattcca gttcgttgat ctggtcgagc ggctggccgt
     2461 ccagcgtgac ggtgatcgtg cggaccgtct tgagcgtgac cggatcgagg aatttgatcg
     2521 tcgccgtgcc gtcgctcatg atcaggttat gcgcgtcggc ggtcaggccc cagccttcgc
     2581 cgcgataatg gaacgtgcgc aacggtttca gcgtggcctg cgaccagacg aagccgacgc
     2641 cggaccgcca ggtcaggctg acgatgcggc ccttcca
//
LOCUS       DASVLL010000491         7078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_193300,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000491 DASVLL010000000
VERSION     DASVLL010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193300"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF402_08175"
     CDS             <1..215
                     /locus_tag="VF402_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX7800312.1"
                     /translation="RITHVTEVLGLEGDVIVTQDLFVYEIEGEDKNGKIVGRHRSTGI
                     GRPRFWDRARYYGLERELAEALDASE"
     gene            222..1217
                     /locus_tag="VF402_08180"
     CDS             222..1217
                     /locus_tag="VF402_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7800313.1"
                     /translation="MIQIILIALLGFVLIASLGFALAGGGGSAQLAKRTQAVGARATT
                     TARRQKGAQARTPEERRRQISDQLKEAERRERKTRLTLRARMLYAGLEPDEKRFWIIS
                     AVLGSVAFFALILLLGKMPFALRLLVAAGGAFAATYGLPRWALGFMAKRRVAKFTAEF
                     PNAMDIIVRGIKSGLPVNDGLKLVAKECSAPLGPEFQRLVENVAIGMSLEQALDKMSE
                     HVPAPELRFFAIVIAIQQKSGGNLSEALGNLSTVLRARKMMREKIKALSSEAIASASI
                     IGCLPPGVALMIFITRPDYISIMFTNVVGNLMLMGGAVWMGLGILMMRKMINFKF"
     gene            1227..2252
                     /locus_tag="VF402_08185"
     CDS             1227..2252
                     /locus_tag="VF402_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7800314.1"
                     /translation="MEAFAAAIVNPGNLTAIVVALMVFFTVVTLGQSLMSGEQLAKRM
                     KSVATRREELRRLSRQGVAREGSALRRTDTSPYRALVEKLNLRTLLEDPKVVEKLAAA
                     GFRGPKPVSTFYFFRFAMPFVLAVGTAFYVFGLNVGHLSLQIKALTCMGAMVLGYYAP
                     NLYISNRASKRRQSIVAAFPDALDLLLISVESGMSIETALHRVAGEIGSASIELAEEL
                     SLLVAELSYLPERRMAYEGLAVRTNHPGVKAVCTAMVQAEKFGTPLGAALRVMAKENR
                     ELRLSAAEKKAAALPAQLTVPMIVFFLPVLFVVILGPVVLRISAMQKHDSSTPVINSH
                     PTPKNPH"
     gene            2335..3903
                     /locus_tag="VF402_08190"
     CDS             2335..3903
                     /locus_tag="VF402_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7800315.1"
                     /translation="MRFIVRAALSASLAALATAPALAAAPSVSLSVDAAKPGATIDRH
                     IFGQFAEHLGHGVYGGVWVGKDSSIPNTRGVRNDVVAALKAIKVPDVRWPGGCYADSY
                     HWRDGVGPERNTRVNTAWGNVVDPNTFGTDEYMDFLSQIGAEAYVSLNIGSGTVGEAQ
                     DWMEYMTADQPTTLEKERAANGHPAPYDVPFMGIGNESWDCGGLMTPDEYTAELRRYA
                     RFVGSYNPKAPTQKIAVGPGTDDTAWTEAVMKTWKERRGWSFDFQGLSLHSYTVYDGW
                     SPHMPSTGFDQTAYAHVLKETLKMGPLIDKQAAVMDKYDPDRKVALVVDEWGAWLAPT
                     PGSNPAFLMQQNTQRDAVLAALNLNIFARHADRVRMANIAQMVNVLQAMILTDGPKMV
                     LTPTYYVFKLYVPFQDATFLPVTYDAGTYTQGDTTLPRLDAIAAKGKDGKIWLSLTNL
                     DPNQPLEVDAAALGFKASSASGQTLSAPKVDSLNDFDHPDVVTPKPIGGKLKNGAVIL
                     TLPPASVTVVSLEP"
     gene            complement(3987..5858)
                     /locus_tag="VF402_08195"
     CDS             complement(3987..5858)
                     /locus_tag="VF402_08195"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX7800316.1"
                     /translation="MSLSEAANRVAPVARKANMMVRPKARPELKLHRSVVTDPSRDAL
                     LTDFGKTTLDDRYLMPGETYQDMFARVATTFADDADHAQRIYDYISKLWFMPATPVLS
                     NGGAARGLPISCFLNAVPDNLEGIVATWNENVALASNGGGIGTYWGGVRSIGEKVKGA
                     GQTSGIIPFIRVMDSLTLAISQGSLRRGSAAVYIDIFHPEIEEFLEIRKSSGDFNRKS
                     LNLHHGIGITDEFMEAVRDGKPFALRSPKNDEVLRYVDARSLWQKILEIRLQTGEPYL
                     IFSDTVNRAMPQHQRELGLKVRQSNLCSEIMLHTGVDHLGRDRTAVCCLSSVNAETFL
                     EWRDAPGFIEDVMSFLDNVLEDFIQQAPSSMEAAIYSAQRERSVGLGLMGFHSFLQRQ
                     GVAFESAMAKSWNMRLFKHLLREADKASVKLAEERGPCPDAAERGVMERFSHKMAIAP
                     TASISIICGGTSAGIEPIPANIYTHKTLSGSFAVKNPYLVELLQAKGQDTAEVWDSIV
                     QHEGSVQHLDFLNEDEKAVFKTAFEIDQRWVVELAADRTPEICQSQSLNIFLPGDVDK
                     WDLHMLHWTAWEQGLKSLYYLRSKSVQRAAHAGSEARESIERMETPKTDYEECLACQ"
     gene            complement(6100..6477)
                     /gene="panD"
                     /locus_tag="VF402_08200"
     CDS             complement(6100..6477)
                     /gene="panD"
                     /locus_tag="VF402_08200"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX7800317.1"
                     /translation="MRQLLRSKIHNAYVTEANLAYIGSITIDEDLMDAVGLWEGERVL
                     VVSNTSGARLETYVIAGARGSGHIAMNGAAAHLIAESEQIIIMGFELTDTPIIPSVVL
                     VDRNNKLDRYLSETPDTILSQKS"
     gene            complement(6622..>7078)
                     /locus_tag="VF402_08205"
     CDS             complement(6622..>7078)
                     /locus_tag="VF402_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7800318.1"
                     /translation="REALVEAAAWHIHAAREAAFEAEARKLKPGQDALDGAIDAYWRL
                     LDSVPFSAFLALERAGRQDEEVARAIAPAQEAFDRGAMGRATPGFIMAGADARFQVSR
                     DLARFALEGMHRTGLTYEHEARVDNVLSVIKRAIHMLNRKGDVSDLWSE"
BASE COUNT         1229 a         2325 c         2281 g         1243 t
ORIGIN      
        1 gccgcatcac ccacgtcacc gaggtgctgg gcctcgaagg cgacgtgatc gtgacgcagg
       61 atctgttcgt ctatgaaatc gagggcgagg acaaaaacgg caagatcgtc gggcgtcacc
      121 gctcgaccgg catcggccgc ccgcgtttct gggaccgcgc gcgctattac ggactggagc
      181 gcgaactggc ggaggcgctg gatgccagcg aatagcgcgt catgattcag attattctga
      241 tcgcccttct cggtttcgtg ctgatcgcca gcctcggctt cgcgctggcg ggcggcggcg
      301 gttcggcgca actggccaag cgcacgcagg cggtcggcgc gcgcgccacc accacggcgc
      361 ggcggcagaa gggcgcgcag gcgcgcaccc cggaggagcg ccgccgccag atcagcgatc
      421 agctcaagga ggccgagcgg cgcgagcgca agacgcgcct caccttgcgc gcgcgcatgc
      481 tgtacgccgg gctggagccg gacgaaaagc gcttctggat catcagcgcc gtgctgggct
      541 cggtcgcctt tttcgcgctg atcctgctgc tgggcaagat gccgttcgcc ctgcggctgc
      601 tggtcgcggc gggcggcgcc ttcgcggcga cctacggcct gccgcgctgg gcgctgggct
      661 tcatggccaa gcgccgcgtc gccaaattca cggcggaatt tcccaacgcg atggacatca
      721 tcgtgcgcgg catcaagtcg ggcctgccgg tcaatgacgg gctgaaactg gtggccaagg
      781 aatgttcggc gccgctgggg ccggagttcc agcgtctggt cgagaacgtc gccatcggca
      841 tgtcgctgga gcaggcgctc gacaagatga gcgagcacgt gcccgcgccg gaactgcgct
      901 tcttcgccat cgtcatcgcc atccagcaga agtcgggcgg caacctgtcc gaggcgctgg
      961 gcaacctgtc gaccgtgctg cgcgcgcgca agatgatgcg cgaaaagatc aaggcgctgt
     1021 cctccgaagc catcgcctcg gcctcgatca tcggctgcct gccgccgggc gtggcgctga
     1081 tgatcttcat cacgcggccc gactatatct cgatcatgtt caccaacgtc gtcggcaatc
     1141 tgatgctgat gggcggcgcg gtgtggatgg gcctgggcat cctgatgatg cgcaagatga
     1201 tcaatttcaa gttctagggg gaccgcatgg aagccttcgc cgccgccatc gtgaaccccg
     1261 gcaacctgac cgccatcgtc gtcgcgctca tggtgttctt caccgtcgtc acgctgggcc
     1321 agagcctgat gtcgggcgaa cagctcgcca aacgcatgaa gtccgtcgcc acccgccgcg
     1381 aggagctgcg ccgcctgtcg cgtcagggcg tggcgcgcga aggcagcgcc ttgcgccgca
     1441 ccgacaccag cccgtaccgc gctttggtcg agaagctgaa cctcaggacg ctgctggaag
     1501 accccaaggt ggtcgagaag ctggccgccg ccggtttccg cggccccaag ccggtgtcga
     1561 ccttctactt cttccgcttc gccatgccgt tcgtgctggc ggtcgggacg gccttctacg
     1621 tgttcggcct gaacgtcggc cacctgtcgt tgcagataaa ggcgctgacc tgcatggggg
     1681 cgatggtgct gggctattac gcgcccaacc tctacatctc caaccgggcc tccaagcggc
     1741 gccagtcgat cgtcgcggcc tttcccgacg cgctggacct gctgctgatc tcggtcgagt
     1801 cgggcatgtc gatcgaaacc gcgctgcacc gcgtggcggg ggaaatcggc tcggcctcga
     1861 tcgagctggc cgaggagctg tcgctgctgg tcgccgaatt gtcctacctg cccgaacgcc
     1921 gcatggccta tgaagggctg gcggtgcgca ctaatcatcc gggcgtcaag gccgtgtgca
     1981 cggcgatggt gcaggccgaa aagttcggca cgccgctggg cgccgccttg cgcgtcatgg
     2041 ccaaggaaaa ccgggaattg cgcctgtccg ccgccgaaaa gaaggccgcc gccctgcccg
     2101 cgcagttgac cgtgccgatg atcgtcttct tcctgcccgt gctgttcgtg gtcatcctcg
     2161 ggccggtcgt gctgcgcatc tcggccatgc agaagcacga ttcgtcgacg ccggtgatca
     2221 attcgcaccc gacgccgaag aacccgcact aagcggcggc ctgttgcgcc cggcgcctga
     2281 gtatggtagc gccatcataa ctcagacata taaacagcaa cagggatctc agtcatgcgc
     2341 ttcatcgtcc gcgccgcgct cagcgcgtcg ctcgccgccc tcgccaccgc tccggcgctg
     2401 gccgccgcac cctccgtctc gctcagcgtc gacgcggcca agcccggcgc cacgatcgac
     2461 cggcatattt tcggccagtt cgccgagcat ctcggccacg gcgtctatgg cggcgtctgg
     2521 gtcggcaagg actcgtcgat ccccaacacg cgcggcgtcc gcaacgacgt ggtcgcggcg
     2581 ctcaaggcga tcaaggtgcc cgacgtgcgc tggcccggcg gctgctacgc cgacagctac
     2641 cactggcgcg acggcgtcgg gcctgagcgc aacacccgcg tcaacaccgc ctggggcaat
     2701 gtcgtcgacc ccaacacctt cggcaccgac gaatatatgg acttcctcag ccagatcggc
     2761 gcggaggcct atgtctcgct caatatcggc tccggcacgg tcggcgaggc gcaggactgg
     2821 atggagtaca tgaccgccga ccagccgacg acgctggaaa aggagcgcgc ggccaacggc
     2881 catccggcgc cctatgacgt gccgttcatg ggcatcggca atgaaagctg ggactgcggc
     2941 gggctgatga cgccggacga atacaccgcc gaactgcgcc gctacgcccg cttcgtcggc
     3001 agctataacc ccaaggcgcc gacgcagaag atcgcggtcg gccccggcac ggacgacacg
     3061 gcctggaccg aggcggtgat gaagacgtgg aaggagcgcc gcggctggtc gttcgacttc
     3121 cagggcctgt cgctgcattc ctacaccgtc tatgacggct ggtcgccgca catgccctcg
     3181 accggtttcg atcagacggc ctatgcccac gtgctgaagg aaaccctgaa gatggggccg
     3241 ctgatcgaca agcaggccgc cgtcatggac aagtacgatc ccgacaggaa ggtcgctctg
     3301 gtcgtcgacg aatggggcgc gtggctggcg ccgacgcccg gctcgaaccc cgccttcctg
     3361 atgcagcaga acacccagcg cgacgccgtg ctggccgcgc tcaacctcaa catcttcgcg
     3421 cgccacgccg accgcgtgcg catggccaac attgcccaga tggtcaatgt gttgcaggcc
     3481 atgatcctga ccgacggccc gaagatggtg ctgacgccga cctattacgt cttcaagctg
     3541 tacgtcccgt tccaggacgc gaccttcctg ccggtgacct acgacgcggg gacttatacg
     3601 caaggggata cgactctgcc gcgcctcgac gccatcgcgg cgaagggcaa ggacggcaag
     3661 atctggctgt ccctgaccaa tctcgacccg aaccagccgc tcgaggtcga cgccgccgcg
     3721 ctgggcttca aggcgtcctc ggccagcggc cagaccctga gcgcgcccaa ggtcgacagc
     3781 ctcaacgact tcgaccaccc cgacgtcgtg acgccgaagc cgatcggcgg caagctgaag
     3841 aacggcgccg tcatcctgac cctgccgccc gcctcggtga cggtcgtgtc gctggagccg
     3901 taaggcgaga cttcgcgagg aaaaagaaag ggaggaaagg accggcgcct ttcctccctt
     3961 tttcatttgt ctccgcgtcg cgttgcttac tggcaggcga gacattcctc gtagtcggtc
     4021 ttgggcgtct ccatccgctc gatggactcg cgcgcttcgg agccggcgtg ggcggcgcgc
     4081 tgcaccgact tggagcgcag gtaatagagc gatttcagcc cctgctccca cgccgtccag
     4141 tgcagcatgt gcaaatccca cttgtcgacg tcgccaggca ggaagatgtt gagcgactgc
     4201 gactggcaga tttccggcgt gcggtcggcg gccagttcga ccacccagcg ctgatcgatc
     4261 tcgaaggcgg tcttgaacac ggccttttcg tcctcgttga ggaagtcgag atgctgcacc
     4321 gagccttcgt gctgcacgat cgaatcccag acctcggcgg tgtcctgccc cttggcctgc
     4381 aacagctcga ccagatacgg gttcttgacc gcgaacgagc cggacagggt cttgtgcgtg
     4441 tagatattgg ccgggatcgg ctcgatgccc gccgacgtgc cgccgcagat gatcgagatc
     4501 gaggcggtcg gcgcgatggc catcttgtgc gagaagcgct ccatcacgcc gcgctcggcg
     4561 gcgtcggggc acggcccgcg ctcctcggcc agcttgacgg aagccttgtc ggcctcgcgg
     4621 agcaggtgct tgaacaggcg catgttccac gacttcgcca tcgccgactc gaacgcgacg
     4681 ccctggcgtt gcaggaagga atggaagccc atcaacccta agcccaccga gcgttcgcgc
     4741 tgcgccgaat agatggccgc ctccatcgag gagggcgcct gctggatgaa gtcctccagc
     4801 acgttgtcga ggaagctcat cacgtcctcg atgaagcccg gcgcgtcgcg ccattcgagg
     4861 aaggtctcgg cgttgaccga cgacaggcag cacacggcgg tgcggtcgcg gcccagatga
     4921 tcgacgccgg tgtgcagcat gatttccgag cacaggttcg actggcgcac cttcaggccc
     4981 agttcgcgct ggtgctgcgg catggcgcgg ttcaccgtgt cggagaagat caggtacggc
     5041 tcgccggttt gcaggcggat ttcgaggatc ttctgccaca gcgaacgcgc gtcgacatag
     5101 cgcagcacct cgtcgttctt gggcgagcgc agcgcgaacg gcttgccgtc gcgcaccgct
     5161 tccatgaatt cgtcggtgat gccgatgccg tggtgcaggt tcagcgattt gcggttgaaa
     5221 tcgcccgacg acttgcggat ttcgaggaac tcctcgattt ccgggtggaa gatgtcgata
     5281 tagacggcgg ccgagccgcg gcgaagcgag ccttgcgaaa tcgccagggt cagcgagtcc
     5341 atcacgcgga tgaacgggat aatgccggag gtctggcccg cgcccttgac cttttcgccg
     5401 atcgagcgca cgccgcccca gtaggtgccg atgccgccgc cgttcgaggc cagcgccacg
     5461 ttctcgttcc aggtcgcgac gatgccttcg agattgtccg gcaccgcgtt caggaagcac
     5521 gagatcggca ggccgcgcgc cgcgccgccg ttcgacaaaa ccggcgtcgc cggcatgaac
     5581 cacagtttgg agatgtaatc gtagatgcgc tgggcgtgat cggcgtcgtc ggcgaaggtg
     5641 gtcgccacgc gcgcgaacat gtcctgatag gtctcgcccg gcatcaggta gcggtcgtcc
     5701 agcgtcgtct tgccgaaatc ggtcagcagg gcgtcgcgcg acggatcggt gacgacggac
     5761 cggtgcagct tcagttccgg ccgcgccttg ggacgcacca tcatattcgc cttgcgtgcg
     5821 acaggagcaa cacgattggc cgcttcactc aaagacatcg tacattcacc ctgatactgg
     5881 agcctcgcgc ttcgccgcgc ccgctgggaa agaggctgtt aacaactaca tctagtgtcc
     5941 gcagcgcccc gtccgccgat atatggggac ataagggccg ctcatcgtca atgggtcagg
     6001 gcgcgtcgtg gtgaagattt ttcgtaaacg aaagcttaat ttttgctttt cgccaagcgg
     6061 ctcaaggggt tgcgaaagct taacactttt tcgccttaat cacgatttct gtgaaagaat
     6121 cgtatcgggc gtttcgctca aatagcggtc aagtttgttg ttgcggtcga ccagcacgac
     6181 actggggata atcggcgtgt cggtcagctc gaagcccatg atgatgatct gctccgattc
     6241 cgcgatcagg tgcgcggcgg cgccgttcat ggcgatatgg cccgacccgc gcgcgcccgc
     6301 gatcacatag gtttccagcc gcgcccccga cgtgttggac accaccagca cgcgctcgcc
     6361 ctcccacaaa ccgaccgcgt ccatcagatc ctcgtcgatg gtgatcgagc cgatataggc
     6421 caggttcgcc tcggtgacgt aggcgttgtg aatcttggaa cgcagtaact gacgcatggg
     6481 agtcccggta tcttgccacg aaaaacacga aaggacacga aagacgcacc ctgatgaagc
     6541 tgcgcgtttg ctaccccatt ttatccgggg cgcgaagcgc ttccttgagt tttcagaacg
     6601 cagacgcttt ttctcggccg atcattccga ccacagatcg ctgacgtcgc ccttgcggtt
     6661 gagcatatgg atggcgcgtt tgatgacgct gagcacgttg tcgacgcgcg cctcgtgttc
     6721 gtaggtcagg ccggtgcggt gcatcccttc cagcgcgaaa cgggccaggt cgcggctgac
     6781 ctgaaagcgc gcgtcggcgc cggccatgat gaagccgggc gtggcgcggc ccatggcgcc
     6841 gcggtcgaag gcttcctgcg ccggggcgat ggcgcgcgcg acctcctcgt cctgccgccc
     6901 ggcgcgctcc agcgccagaa aggccgaaaa cggcacggaa tcgagcagcc gccaataggc
     6961 gtcgatggcg ccgtccagcg cgtcctgacc gggtttcagc ttgcgggcct cggcctcgaa
     7021 cgcggcctcg cgcgcggcgt ggatgtgcca ggcggccgcc tcgaccagcg cctcgcgc
//
LOCUS       DASVLL010000492         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_463332,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000492 DASVLL010000000
VERSION     DASVLL010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463332"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF402_08210"
     CDS             <1..226
                     /locus_tag="VF402_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800319.1"
                     /translation="AAMSLAFLQSLMRLIVASYLAINLWLAFRKGWTTGLWKMRVERV
                     REPRTFWFTVGVYALTLALMAVALLARHGF"
     gene            complement(467..1330)
                     /locus_tag="VF402_08215"
     CDS             complement(467..1330)
                     /locus_tag="VF402_08215"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7800320.1"
                     /translation="MKVCFAGLGVMGAPMAGHLIKDGHEVAGYNRTFAKTEDWAARYG
                     GRAARTMAEAAAQADALLLCVGRDGDVRETIAAALPHLKPGALVIDHTTTSAKLAREM
                     AALCAGKGVVFFDAPVSGGQAGAENGQLSVMAGGDAGRFDDVRALTRPYTKAITLLGE
                     AGAGQTAKMCNQIAIAGAVQGVAEALHFARSAGLDTDLVFDAISAGAAGSWQMTNRWK
                     TMSEDRYDFGFAVDWMRKDLGIALDEARENGAHLAVAALVDQFYSEVQAMGGRRWDTS
                     SLHARLEARRK"
     gene            complement(1327..1812)
                     /locus_tag="VF402_08220"
     CDS             complement(1327..1812)
                     /locus_tag="VF402_08220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7800321.1"
                     /translation="MDKKALRHTLGGFATGVCVITAPDPDHVGGHVIGMTANSFSSVS
                     LTPPLVQWCLDLKAHRYDVYAKAPRFGVNVLGAGQAVLSRRFARENAHILPEDGLSDA
                     AAPLRLREAAAFLECETYDSRVLGDHLVIVGRVLAFDSDPRRAGLLFFRGRYGDTGAM
                     L"
     gene            1978..2883
                     /locus_tag="VF402_08225"
     CDS             1978..2883
                     /locus_tag="VF402_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX7800322.1"
                     /translation="MANRDQPIIIKKVRKGGGAGHHGGAWKVAYADFVTAMMAFFLLM
                     WLINTTSPEQKRGVADYFAPANVSQSTSGSGGILGGQALGTDGDKNEGTMSFIEQMAP
                     KPPDVQDRDGQSSQVKGKANNASDADSDQETDAQKREQMAFQSAAESLRQALQDMPEL
                     AELSKNILVDQTPEGLRIQLIDQEGRSMFDTNSAQPNDRAKLLLRAVAKVINQLPNRI
                     SITGHTSATPGATAQNPRDWALSAARADAARAVLQAAGVDQDRIAQVSGKAASEPLYP
                     DDPSLAGNRRIAIVLLREAPVLPQN"
     gene            2966..3697
                     /locus_tag="VF402_08230"
     CDS             2966..3697
                     /locus_tag="VF402_08230"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX7800323.1"
                     /translation="MSEHFPGRKLRYYLTVPGPCPYLPGQEERKVFVHLPPLEAIGVN
                     DQLSAIGFRRSQNIAYRPACQTCRACQSVRIPVRRYALSRSDRRVVRANADLSKAFVE
                     AEASREQFDLLLKYLRARHPDGGMMDMSWPDLVAMIEDTLVRTHLIEYRLGERLVACV
                     LVDALNDGLSLVYSFYDPELPARSLGRYVILDHIDQASRAGYDYVYLGYWVKGSPKMD
                     YKARYWPLEGLTDLGWQTLPKPVKS"
BASE COUNT          666 a         1348 c         1158 g          627 t
ORIGIN      
        1 ggccgcgatg agcctcgctt ttctccaaag cctgatgcgc ctgatcgtcg ccagctatct
       61 ggcgatcaat ctctggcttg ccttccgcaa gggatggacg accggcctat ggaaaatgcg
      121 ggtcgagcgc gtgcgcgaac ccagaacctt ctggttcacg gtgggcgtct atgctctcac
      181 cctggccctg atggccgtgg cgttgctggc tcggcatggg ttttaggttt ttgccactaa
      241 agacacgaaa cggcacgaaa tccgcgcata gcgccttgtc gcctgttggt cgctgacggt
      301 cgttttttaa accacggaaa acacggaaat cgctaatctt ctgacgccgc gaagcggcgt
      361 ttccgtgttt tccgtggtta aatcttacgg acgctcagga cagacatata cgacaaggcg
      421 ctacgcgcgg atttcgtgcc ttttcgtgcc tttagtggct aaaaaactac ttccgccgcg
      481 cctccaaacg cgcgtgcagg ctggaggtgt cccaccgccg cccccccatt gcctgcacct
      541 cggaatagaa ctggtccacc aaggccgcca cggccagatg cgcgccgttc tcgcgcgctt
      601 cgtcgagcgc tatgcccaga tccttgcgca tccagtcgac cgcgaagccg aagtcgtagc
      661 ggtcctcgct catcgtcttc cagcggttcg tcatctgcca cgatccggcc gcgcccgccg
      721 agatggcgtc gaacacgagg tccgtgtcca gcccggcgct gcgggcgaaa tgcagcgcct
      781 cggccacgcc ctgcacggcg cccgcgatgg cgatctggtt gcacatcttg gcggtctgtc
      841 ccgcgcccgc ttcgcccagc aaggtgatgg ctttcgtata gggccgggtc agcgcccgca
      901 cgtcgtcgaa gcgccctgcg tccccgcccg ccatcaccga caactggccg ttctcggccc
      961 cggcctgccc gcccgacacc ggcgcgtcga agaagacgac gcccttaccg gcgcacaggg
     1021 ccgccatctc gcgcgccagc ttcgccgagg tcgtcgtgtg gtcgatcacc agagcgcccg
     1081 gcttcaggtg cggcagcgcc gccgcgatcg tctcgcgcac atcgccgtcg cgcccgacgc
     1141 acagcagcag cgcgtcggcc tgcgccgccg cctccgccat cgtgcgcgcc gcccggccgc
     1201 catagcgcgc ggcccagtcc tccgtcttgg cgaaggtgcg gttatagccc gccacctcat
     1261 gcccgtcctt gatcagatgc ccggccatcg gcgcgcccat cacgcccagt cccgcgaaac
     1321 acactttcat agcatcgctc ccgtatcgcc atagcgcccg cggaaaaaca gcagccccgc
     1381 gcgccgcgga tcgctgtcga aggccagcac ccggccgacg atgaccagat ggtcgcccag
     1441 cacgcggctg tcgtaggtct cacactccag aaacgccgcc gcctcgcgca gccgcagcgg
     1501 cgcggccgcg tcgctcagcc cgtcctcggg caggatatgc gcgttctcgc gcgcgaaccg
     1561 gcgcgacagc acggcctgcc cggcgcccag cacgttgacg ccgaagcgcg gcgccttggc
     1621 gtaaacgtcg taacggtgcg ccttcagatc gaggcaccac tgcaccaaag gcggcgtcag
     1681 cgacaccgag ctgaaggaat tggcggtcat gccgatgaca tggccgccga catgatcggg
     1741 gtccggcgcg gtgatgacgc agacgccggt ggcgaagccg cccagcgtgt ggcgcagagc
     1801 ctttttatcc ataatctcaa ccagaaaaac gcacccttca ctaagccgtt ccggcaggcg
     1861 aaagcaacct ttccgaggat ttttgtcgcg ccgccgcgat ccggccccga cgcctcaacc
     1921 ccctattaac gttaagacgg ctaaggtcgg ggcggatttc tcaggttttg cggcgtcatg
     1981 gccaatcgcg atcaacccat catcatcaag aaggtcagga aaggcggcgg cgccggtcac
     2041 cacggcggcg cgtggaaggt cgcctatgcc gacttcgtga ccgcgatgat ggccttcttc
     2101 ctcctgatgt ggctgatcaa caccacctcg ccggagcaga agcgcggcgt cgccgactat
     2161 ttcgccccgg ccaacgtgtc gcaatcgact tccggctccg gcggcatcct cggcgggcag
     2221 gcgctcggca cggacggcga caagaacgaa ggcaccatgt ccttcatcga gcagatggcg
     2281 cccaagccgc ccgacgtgca ggaccgcgac ggccagtcga gccaggtcaa gggcaaggcc
     2341 aacaacgcct ccgacgccga cagcgatcag gagaccgacg cccagaagcg cgagcagatg
     2401 gccttccaga gcgcggcgga atccctgcgt caggccttgc aggacatgcc cgaactggcc
     2461 gagctgtcga agaacatcct ggtggaccag acgccggagg gcctgcgcat ccagttgatc
     2521 gatcaggaag gccgctcgat gttcgatacg aactcggccc agcccaacga ccgcgccaag
     2581 ctgctgctgc gcgcggtggc caaggtgatc aaccagcttc ccaaccgcat ctcgatcacc
     2641 ggccacacct cggccacgcc gggcgcgacg gcgcagaatc cgcgcgactg ggcgctgtcc
     2701 gccgcgcgcg ccgacgccgc ccgcgccgtg ctgcaggcgg cgggcgtcga tcaggaccgc
     2761 atcgcgcagg tgtccggcaa ggccgccagc gaaccccttt accccgacga tccgtctttg
     2821 gcgggcaatc gccgcatcgc catcgtgctg ctgcgcgaag cgccggtgct gccgcaaaat
     2881 taaacccggc gcccgcttgc cggatgggac gaattggttt caaataaggt ctaatcaccc
     2941 gcgcggcttc aggacatccg gccatatgag cgagcatttc cccggacgca aactcagata
     3001 ctatctgacc gttcccggtc cctgcccgta tctgcccggt caggaggagc gcaaggtctt
     3061 cgtgcatctg ccgccgctgg aggccatcgg cgtcaacgat caattgtcgg ccatcggctt
     3121 ccgccgctcg cagaacatcg cctaccgccc cgcctgccag acctgccgcg cgtgccagag
     3181 cgtgcgcatc ccggtgcggc gctatgcgct gtccagatcc gaccgccgcg tcgtgcgcgc
     3241 caacgccgac ctttccaagg cgttcgtcga ggccgaggcc agccgcgaac agttcgacct
     3301 gctgctaaaa tacctgcgcg cgcgtcatcc cgacggcggc atgatggaca tgagctggcc
     3361 cgacctcgtg gccatgatcg aggacacgct ggtgcgcacg cacctgatcg agtaccggct
     3421 gggcgagcgg ctggtcgcct gcgtgctggt cgacgcgctc aatgacggcc tgtccctggt
     3481 gtacagcttc tacgacccgg agcttccggc gcgcagcctc ggccgctacg tcatcctcga
     3541 ccatatcgat caggccagcc gcgcgggcta cgactatgtc tatctcggct actgggtcaa
     3601 gggctcgccc aagatggact acaaggcccg ctactggccg ctggaagggc tgaccgacct
     3661 cggctggcag acattgccga agccggtcaa atcctgatca gggccgggcg ggcgtcgcgt
     3721 cggatgacgc gctgtccgaa gtcgctgtgg ttgaagacgc cgcttcgggc gacacctccg
     3781 cagacgacca ttgcgatga
//
LOCUS       DASVLL010000493         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_408059,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000493 DASVLL010000000
VERSION     DASVLL010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408059"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VF402_08235"
     CDS             <1..735
                     /locus_tag="VF402_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7800324.1"
                     /translation="YIAALERRVIAPDLRGRGLSENDPDAGNYHLWTYARDVAQMCDA
                     FGIGQAVVIGSSMGGLAAMVLATLRPHLVRAAVLNDVGPKPARKGLARIAARAGADPA
                     PHDDWAGLAAALAEQSGEDYPNYKAADWLNMARRLYRKGRDGRLHPAFDARLTAACRH
                     LTLDLAAYDLEPCYASLAHRRPVLLLHGAQSDLIGEKEIRAMKRVSRAGGGDFTRVDV
                     SHVGHAPDLSEAQSRLAIQDFLDALP"
     gene            793..1194
                     /locus_tag="VF402_08240"
     CDS             793..1194
                     /locus_tag="VF402_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX7800325.1"
                     /translation="MIFDSPYLLGFDEMRLLIERVERNHDAYPPYNVEALSPDALRIS
                     IAVAGFAQDQLRIEARGAHLTVSALRDKTEAAGRDYLHRGIALRGFNRAFVLSEGWKV
                     ADASLAHGLLHIDLTRPRPDGETQTIPIRAG"
     gene            complement(1191..1655)
                     /gene="ptsN"
                     /locus_tag="VF402_08245"
     CDS             complement(1191..1655)
                     /gene="ptsN"
                     /locus_tag="VF402_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX7800326.1"
                     /translation="MSLANLLDRNAILGHVSVNSKRQALQVVADVVARNLNLDAADVL
                     PALLEREKLGSTGVGLGVAVPHAALKGLDRMYGVFARLETPVAYEAIDDMPVDLVFAL
                     LAPEDAGTEHLRALAKVSRLLRQKDLREQLRAIENPDAIYALLTGMADTNVA"
     gene            complement(1815..2429)
                     /gene="raiA"
                     /locus_tag="VF402_08250"
     CDS             complement(1815..2429)
                     /gene="raiA"
                     /locus_tag="VF402_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7800327.1"
                     /translation="MQIQVSGKQVEVGEALRERIESELSQGVAKYFERGGVAEVTISK
                     QGYLFKADCLVRLASGQVLVSHAFGGDAHSAFSGTLDNMEKRVRRYKRRLKSHHNGAP
                     QKEEIANVTVLRAGDLDGVNADDDMEDGFDHATEPQAMVIAETEAPLRTMTVSGAVAE
                     MEMSNYPVVMFRNAAHGGFSVIYRRPDGNIGWIDPERTNVRGHA"
BASE COUNT          393 a          837 c          803 g          418 t
ORIGIN      
        1 tacatcgccg cgcttgagcg ccgggtcatc gcgccggacc tgcgcgggcg cggcctgtcc
       61 gagaacgatc ccgacgcggg caattatcat ctgtggacct atgcgcgcga cgtggcgcag
      121 atgtgcgacg ccttcggcat cggccaggcg gtggtgatcg gcagttcaat gggcgggctg
      181 gcggcgatgg tgctggccac cttgcgcccg catctggtgc gcgccgccgt gctgaacgat
      241 gtcgggccga agcccgcgcg caaggggctg gcgcgcatcg ccgcgcgcgc cggcgccgat
      301 ccggcgccgc atgacgactg ggccgggctg gccgccgcgc tggccgaaca atcgggcgag
      361 gactatccga actataaggc ggccgactgg ctgaacatgg cgcggcgcct ttatcgcaag
      421 gggcgcgacg gccggttgca tccggccttc gacgcgcgcc tgaccgccgc ctgccgccat
      481 ctgacgctcg atctggccgc ctacgatctg gagccgtgct acgccagtct ggcgcaccgg
      541 cgcccggtcc tgctgctgca cggcgcgcag tccgacctga tcggcgaaaa ggagatccgc
      601 gccatgaagc gcgtcagccg cgcgggcggc ggcgacttca cccgcgtcga cgtcagccat
      661 gtcggccatg cccccgacct gagcgaggcc cagtcgcggc tggcgattca ggatttcctc
      721 gacgccttgc cctaggcggc gctccgcccc atatctttcg gcgtaaaggc ggagttcagc
      781 atggcgcgcg gcgtgatatt cgacagtccc tacctgctgg gcttcgacga gatgcgcctg
      841 ctgatcgagc gcgtcgagcg caaccatgac gcctatccgc cctataatgt cgaggccctg
      901 tcccccgacg ccctgcgcat cagcatcgcg gtcgccgggt tcgcgcagga ccagttgcgc
      961 atcgaggcgc gcggcgccca tctcacggtc agcgctctgc gcgacaagac cgaggcggcc
     1021 ggacgcgact acctgcatcg cggcatcgcc ctgcgcggct tcaaccgcgc cttcgtattg
     1081 tcggaagggt ggaaggtggc ggacgccagc ctcgcgcacg gcctgttgca tatcgacctg
     1141 acgcggcccc ggcccgacgg cgagacgcag acgataccga tccgagcggg ttaagctaca
     1201 ttcgtatccg ccatgccggt cagcagggcg tagatggcgt cggggttttc tatggcgcgc
     1261 agttgttcgc gcaagtcctt ctgccgcaac aggcgcgaca ccttggccag ggcgcgcaag
     1321 tgctccgtgc ccgcgtcctc cggcgccagc agcgcgaaga ccagatcgac cggcatgtcg
     1381 tcgatggcct cataggcgac cggcgtctcc agacgcgcga acacgccgta catgcggtcc
     1441 agccccttga gcgcggcgtg cggcacggcg acgcccagcc ccaccccggt cgagccgagc
     1501 ttttcgcgct ccagcagcgc cggcagcacg tcggcggcgt cgagattcag attgcgcgcg
     1561 acgacgtcgg ccacgacctg aagcgcctgg cgcttggaat tgacgctgac atggcccaga
     1621 atggcgttgc ggtcgagcag gttggctaaa gacataatcc gtccgtgaag gcttaattgg
     1681 gcgcatccgt aacgacgtta acgccgcgcg ttgccttaaa ggggcgggcc gcaagcgtca
     1741 acccaccccc tgatgacaag tttttgactg gctttcagcc catttcgcgt atcgcgcgca
     1801 cgcaaacggc gcgtttacgc gtggccgcgc acattggtgc gctccggatc gatccagccg
     1861 atattgccgt cgggacggcg atagatgacc gaaaagccgc catgcgcggc attgcggaac
     1921 atcacgaccg gatagttgga catctccatt tcggccacgg cgccggagac ggtcatggtg
     1981 cgcagcggcg cctcggtttc ggcgatgacc atggcctgcg gctccgtcgc atggtcgaag
     2041 ccgtcctcca tgtcgtcgtc ggcattcacg ccgtcgagat cgccggcgcg cagcaccgtg
     2101 acattggcga tctcctcctt ttgcggcgcg ccgttgtggt ggctcttcag ccgccgctta
     2161 tagcggcgca cgcgcttctc catattgtcg agcgtgccgg agaaagcgga atgggcgtcg
     2221 ccgccgaagg cgtgggacac cagcacctga cccgaggcca ggcgcaccag acagtcggcc
     2281 ttgaagagat agccctgctt cgagatcgtg acctcggcca cgccgccgcg ctcgaaatac
     2341 ttggcgacgc cttgcgacag ctcgctttcg atgcgctcgc gcagcgcctc gccgacttca
     2401 acctgttttc cgctgacttg aatttgcata gcgaaatacg ctccgttctt a
//
LOCUS       DASVLL010000494         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_86858, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000494 DASVLL010000000
VERSION     DASVLL010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86858"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..397)
                     /locus_tag="VF402_08255"
     CDS             complement(137..397)
                     /locus_tag="VF402_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEX7800328.1"
                     /translation="MITKIVTAAAIYIMIWWVSLFAVLPLGGDPSHHEAGVPVQKGND
                     PGAPVRHNLGKKMRLNSLVAFVLWLVAMAFMYLIHLPLPEIR"
     gene            complement(451..1392)
                     /gene="argC"
                     /locus_tag="VF402_08260"
     CDS             complement(451..1392)
                     /gene="argC"
                     /locus_tag="VF402_08260"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7800329.1"
                     /translation="MAYKVFIDGEAGTTGLQIRERLEKRADIELIRLADAVRKDAAAR
                     KAALNGAHAVILCLPDEAAREAVSLIDNPDVAVIDASTAHRVAEGWTYGFPEMPNGDE
                     TPQRDLIAASKRISNPGCHATGFIALTRPLVEAGLIAPFFPIMATSLSGYTGGGKSMI
                     AEFESGEGHERIYATGLMHKHLPEMTLYSGLESPPIFSPKVGRFPQGLIVEVPLPLWA
                     LPDAPDLETVHDALMAAYDGERFVNVASLEESRATGSVDADSLANTNQLLIHVFGDPE
                     AETANLVAVFDNLGKGASGAAVQNLNLVLGVDEGLGL"
     gene            complement(1656..2144)
                     /gene="rpsI"
                     /locus_tag="VF402_08265"
     CDS             complement(1656..2144)
                     /gene="rpsI"
                     /locus_tag="VF402_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7800330.1"
                     /translation="MSEVQAPAQGLEALASLSSNPEAAPVERVQQLDKLGRAYATGKR
                     KNAIARVWIKPGSGKWIINGREQEVYFARPVLRMMIAQPLNLTDRATQFDIMVSVQGS
                     GLSGQAGAIRHGLSKALTYYEPGLRPALKAAGFLTRDSRVVERKKYGRAKARRSFQFS
                     KR"
     gene            complement(2144..2617)
                     /gene="rplM"
                     /locus_tag="VF402_08270"
     CDS             complement(2144..2617)
                     /gene="rplM"
                     /locus_tag="VF402_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7800331.1"
                     /translation="MLTSTTAALKPADVEKKWIVIDAEDAVLGRLAAFIAMRLRGKHL
                     PNFTPHTDGGDNVIVINAEKVHLTGKKHNEIFYWHTGHPGGVKQRTLGQLLNGKYPER
                     VLTKAVERMMPKESPLARKQMTQLRIYAGATHPHEAQTPEVIDFKALSRKNVRSA"
BASE COUNT          502 a          893 c          897 g          510 t
ORIGIN      
        1 ttgagcaggg gcagaaaaaa gcgcgacagg cgcatgggca gagaatccgt cagtttgagg
       61 ttggcgcaag gctaccgggc ctgcgcctta tcgaaaagcg tttttgcaaa caaaatgcgg
      121 cggaaacgcg cccgcgtcag cgaatttccg gcaggggcag atggatcagg tacatgaacg
      181 ccatcgccac cagccacagc acgaaggcga ccagcgagtt gagccgcatc ttcttgccga
      241 gattgtgccg caccggcgcg ccgggatcgt tgcccttctg caccggcacg cccgcctcat
      301 gatggctggg gtcgccgccc agcggcagga ccgcgaacag cgagacccac cagatcatga
      361 tgtaaatcgc ggcggcggtg acgatcttgg tgatcatggc ggcgtatcct tcccggcttc
      421 gccggtcggt ccacgccgcc gacggttctt ttacagaccc agcccttcgt ccacccctaa
      481 taccagattg aggttttgta cagccgcgcc cgacgcgccc ttgccgaggt tgtcgaacac
      541 cgccaccaga ttggccgtct cggcctccgg gtcgccgaag acgtggatca acagttgatt
      601 ggtgttggcc aggctgtccg cgtcgaccga tccggtcgcg cgcgactctt cgagcgacgc
      661 cacattgacg aagcgctcgc cgtcataggc cgccatcagc gcgtcgtgga ccgtctcaag
      721 gtccggcgcg tccggcagcg cccacaaggg cagcggaacc tcgacgatca gcccctgcgg
      781 gaagcgcccg accttggggc tgaagatcgg cggcgattcc agcccgctgt agagcgtcat
      841 ttccggcaga tgcttgtgca tcaggccggt ggcgtaaatg cgctcatggc cctcacccga
      901 ctcgaattcc gcgatcatcg acttgccgcc gccggtatag cccgacaggg aggtcgccat
      961 gatcgggaag aacggcgcga tcagcccggc ctcgaccagc gggcgcgtca gggcgatgaa
     1021 gccggtggcg tggcagccgg gattgctgat ccgcttcgac gccgcgatca ggtcgcgctg
     1081 cggcgtttcg tcgccgttcg gcatttccgg gaagccatag gtccagccct cggcgacgcg
     1141 atgcgcggtc gaggcgtcga tcaccgccac atccgggtta tcgatcaggc tcaccgcctc
     1201 gcgcgccgcc tcgtccggca ggcacaggat cacggcgtgc gcgccgttca gcgccgcctt
     1261 acgcgccgcc gcgtccttgc gcacggcgtc cgccagacgg atcagctcga tatcggcgcg
     1321 tttttcaagc cgctcgcgaa tttgcagacc cgtggtcccg gcctcgccgt cgatgaaaac
     1381 cttatacgcc ataggacgct cccgatcaga acttaaagta aattctcaaa accttccctc
     1441 tcccctccat gaggggagag gacgaggagc aaggcgtagc cgccgatcct cgggtgaggg
     1501 gccacccgcc gactgagcgc aggcccctca cccgatcgcc ttcgcttacg ctcggctctc
     1561 gtcctctccc cgtaaacggg gagaggggta gtaaaagcaa aaaaagcgct ccggttgccc
     1621 gaagcgcttt tcgcagaacc gtatgacccg tgcgattaac gcttcgagaa ctggaaggaa
     1681 cggcgggcct tggcgcggcc gtacttcttg cgttcgacga cgcgcgagtc gcgggtcagg
     1741 aagccggcgg ccttcagggc cgggcgcagg cccggttcat aataggtcag cgccttggac
     1801 aggccgtggc ggatggcgcc ggcctgaccc gacaggcccg aaccctgcac cgacaccatg
     1861 atgtcgaact gcgtggcgcg gtcggtcagg ttgagcggct gggcgatcat catgcgcagc
     1921 accgggcggg cgaaatagac ttcctgctcg cggccgttga tgatccactt gccggagccc
     1981 ggcttgatcc agacgcgcgc gatggcgttc ttgcgcttgc cggtggcata ggcgcggccc
     2041 agcttgtcga gctgctgaac gcgctcgacc ggggctgctt ccgggttgga ggacagcgaa
     2101 gcgagggctt ccaggccctg ggcgggagct tgaacttcag acattaggcg ctccggacgt
     2161 tcttgcggct cagcgccttg aagtcgatga cttccggcgt ctgggcttcg tggggatgag
     2221 tggcgccggc gtagatgcgc agttgcgtca tctgcttgcg ggccagcggg ctttccttcg
     2281 gcatcatgcg ctcgaccgcc ttggtcagca cgcgctccgg atacttgccg ttcagcagtt
     2341 ggccaagcgt gcgctgcttg acgccgcccg gatggccggt gtgccagtag aagatttcgt
     2401 tgtgcttctt gccggtcagg tgaaccttct cggcattgat cacgatgacg ttgtcgccgc
     2461 cgtcggtgtg cggcgtgaag ttcggcagat gcttgccgcg aagacgcatg gcgatgaaag
     2521 cggcgagacg gcccagcacg gcgtcctcgg cgtcgatcac gatccacttc ttctcgacat
     2581 cggcgggctt gagcgccgca gtggtcgaag tcaacatggg gtcagtttcc ttgataagcc
     2641 atgccccaca gaccgggacg cagcgaagag gcgcgtcatg tacatgagcg cccccaccct
     2701 gtcaacgcgg aattttcaac caaacgcggc tgatggcatt gaattgtaag ggaaaattgc
     2761 actaggtatt aaattaccgc aacatttccc cgcccctcag aa
//
LOCUS       DASVLL010000495         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_354988,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000495 DASVLL010000000
VERSION     DASVLL010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354988"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="ettA"
                     /locus_tag="VF402_08275"
     CDS             <1..1143
                     /gene="ettA"
                     /locus_tag="VF402_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX7800332.1"
                     /translation="LSKPDMLLLDEPTNHLDAESVAWLQHHPENFPGCVILVTHDRYF
                     LDQVTKWTLELDRGKGVPYEGNYSGWLEQKQKRVVQEQSESEARQRALTKELEWVRSG
                     AKARQAKSKARLAAYEDMVREQENARQAQTFATIQIPPGPRLGNVVLEVEGLEKEYGD
                     KLLFKDLTFRLPPNGIVGVIGPNGAGKSTLFKLITGQEQPDKGTIRLGETVKLAYVDQ
                     SRDDLNPNHTVWEAISGGTDIMVVGKKEINSRAYVGGFNFKGGDQQKKVGLLSGGERN
                     RVHLAKTLAAGGNVILLDEPTNDLDIETLQALEEALEEFAGCAVVISHDRWFLDRLAT
                     HILAFEGDSHVEWFEGNFEMYEEDKKRRLGADSLIPHRIKFQKFSR"
     gene            1476..>2134
                     /locus_tag="VF402_08280"
     CDS             1476..>2134
                     /locus_tag="VF402_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800333.1"
                     /translation="MSENKSIFIATPCFGGVVTQAYMQSVIALMQYKAEPPLHLTLAL
                     LGNDALITRSRNTLVSSFLNDTSATHLLFIDADISFAPAQVMRLIEAGKEVVGAMYPI
                     KALDWETAAHRLSRGLETAEEAAMHYVGAPLQDHRARRDGDFVTAAYAGTGMLLVTRA
                     AIEKMIKAYPHLQYAAIHSYPREQRTPATQYALFECLIDRDTGLYLSEDYAFCQRWRD
                     IG"
BASE COUNT          412 a          703 c          664 g          355 t
ORIGIN      
        1 ttgtcgaagc ccgacatgct gctgctggac gaaccgacca accacctcga cgccgagtcg
       61 gtggcgtggt tgcagcacca tccggaaaac ttccccggct gcgtgatcct ggtcacccac
      121 gaccgctact tcctcgatca ggtgaccaag tggacgctcg aactcgaccg cggcaagggc
      181 gtgccgtatg agggtaacta ttccggctgg ctggagcaga agcagaagcg cgtggtgcag
      241 gaacagtccg aatccgaggc gcgccagcgc gcgctgacca aggagctgga atgggtgcgc
      301 tccggcgcca aggcgcgtca ggccaagtcg aaggcccgtc tggccgccta tgaggacatg
      361 gtgcgcgaac aggagaacgc gcgtcaggcc cagaccttcg ccaccatcca gattccgccc
      421 ggaccgcgcc tcggcaatgt cgtgctggag gtcgaggggc tggaaaagga atatggcgac
      481 aagcttttgt tcaaggatct gaccttccgc ctgccgccca acggcatcgt cggcgtgatc
      541 ggtccgaacg gcgcgggcaa gtcgaccctg ttcaagctga tcaccgggca ggaacagccc
      601 gacaagggca cgatccgcct cggcgagacg gtcaagctgg cctatgtcga tcagtcgcgc
      661 gacgacctca atcccaacca tacggtctgg gaggcgatct ccggcggcac cgacatcatg
      721 gtggtcggca agaaggagat caattcacgc gcctatgtcg gcggcttcaa cttcaagggc
      781 ggcgaccagc agaagaaggt cggcctcctg tcgggcggcg agcgcaaccg cgtccacctg
      841 gccaagacgc tggcggcggg cggcaacgtc atcctgctcg acgaaccgac caacgacctc
      901 gacatcgaaa ccttgcaggc tctggaagag gcgctggagg agttcgcggg ctgcgccgtg
      961 gtcatcagcc acgaccgctg gttcctcgac cgtctggcca cccacatcct cgccttcgag
     1021 ggcgattcgc acgtcgaatg gttcgagggc aatttcgaga tgtacgagga agacaagaaa
     1081 cgccgcctcg gcgccgacag cctgatcccg caccggatca agttccagaa gttcagccgg
     1141 taagtccaca aggccgcaac gttttcgtcg cggccttgtt tataggcgtg gatagtcttc
     1201 gagggccaga gtctcggtct gcgaagttcc cccttccgtc accgcctccg gcggcgctgc
     1261 cttccccgta aacggggaag gcgggatctt tgttcgccct ccctgcgcag cggggaggag
     1321 ggaccgccga aggcggtggg aggggccttt cttccgccgg accgcgaagc ccctttataa
     1381 cgcccgcgac acgcccatcg ccctaaccct gccctgcaat caccctaggg tcacggcttc
     1441 ggttgggagc cacagtcaag gggcgcattt catacatgtc ggaaaacaaa agcattttca
     1501 tcgctacgcc ctgcttcggc ggcgtcgtga cgcaggccta tatgcagtcg gtcattgcgc
     1561 tgatgcaata taaggccgag ccgccgcttc acctgactct ggccctgctc ggcaacgacg
     1621 cgctgatcac gcgcagccgc aacacgctgg tctccagctt tctcaacgac acgagcgcca
     1681 cgcaccttct gttcatcgac gccgacatca gcttcgcgcc ggcgcaggtg atgcggctga
     1741 tcgaagccgg caaggaggtg gtcggcgcca tgtacccgat caaggcgctg gactgggaga
     1801 ccgccgcgca ccgcctgtcg cgcgggctgg agacggcgga ggaggcggcc atgcactatg
     1861 tcggcgcccc gttgcaggac catcgcgcgc gccgcgacgg cgatttcgtc accgccgctt
     1921 atgccgggac cggcatgttg ctggtgacgc gggccgccat cgaaaagatg atcaaagcct
     1981 atccgcacct gcaatacgcc gccatccatt cctatccgcg cgaacagcgc acgcccgcga
     2041 cgcaatacgc cttgttcgag tgcctgatcg accgcgacac cgggctttat ctcagcgagg
     2101 actacgcctt ctgccagcgc tggcgcgaca tcgg
//
LOCUS       DASVLL010000496         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_464065,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000496 DASVLL010000000
VERSION     DASVLL010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464065"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VF402_08285"
     CDS             complement(<1..1658)
                     /locus_tag="VF402_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX7800334.1"
                     /translation="MTQTLPTDAASGLPIWDLSDLYDGPEDPRIREDFARGERHVKEL
                     ARLEGAFLSARANAERLGLLIDQAAGLYEKAMDDMGGALAYATLSSTIDRENPAVAKM
                     QSDLSSQAAMISAGALFLSLELNQLEDWEIEQALRATERAQRWRPWLRRIRASRPHEL
                     SADLERLMLERMPATAQWTRLFDETLTRLRARVGKENLTLNEALTRLSAPQAATRKAA
                     AKGLSAALAENAPVLALALNTLAFEKQIEDRWRKYDDPAASRHLANEVDKDAVDAMAE
                     AVGESYARLSHRYYALKARIMGRKSLDFWDRNAPLSEDVQKSYTWDEAKRIVLGAFAQ
                     MGPEFVRRAQLFFDHAWIDAAPRAGKSSGAYAHPVTANRHPYVMLNFTGDRREVLTLA
                     HELGHAVHQTLCQPLGSILADTPLTLAETASIFAEGLVFEHLVADASPEDKKALLAGK
                     IEDGLNSVIRQIAFHRFEEKFHAARQDGEVALDDINAIWLEVMGESLGPAIKLNEGYE
                     VWWAYVSHFVHAPFYVYAYAFGDLLVSALMEKRREDPAAFTPLY"
     gene            complement(1703..>2016)
                     /locus_tag="VF402_08290"
     CDS             complement(1703..>2016)
                     /locus_tag="VF402_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7800335.1"
                     /translation="YSVVHDYCGHGIGREMHEAPQILHFGKPGTGPALHAGMTFTIEP
                     MLNRGRRHVKTEDDGWTVVTRDGSLSAQFEHTVAVTASGAAVLTLRHDESATGPGGSG
                     V"
BASE COUNT          311 a          659 c          697 g          349 t
ORIGIN      
        1 tcgtacagcg gcgtgaaggc cgccgggtct tcgcggcgct tctccatcag cgccgacacc
       61 agaaggtcgc cgaacgcata ggcatagaca tagaagggcg cgtggacgaa gtggctgaca
      121 taggcccacc acacctcata accttcgttg agcttgatcg cggggccaag ggattcgccc
      181 atgacctcca gccagatggc gttgatgtcg tcgagcgcga cctcgccgtc ctgacgcgcg
      241 gcgtggaatt tctcctcgaa acggtggaag gcgatctggc ggatcaccga gttcagcccg
      301 tcctcgatct tgccggccag cagcgccttc ttgtcctccg gcgaggcgtc ggccaccaga
      361 tgctcgaaca ccagcccctc ggcgaagatc gaggccgtct cggccaaagt cagcggcgtg
      421 tcggccagaa tcgagccgag cggctggcac agggtctgat gcaccgcgtg gcccagttcg
      481 tgcgccagag tcagcacctc gcggcggtcg ccggtgaagt tgagcatcac gtacggatgg
      541 cggttggccg tgaccggatg ggcgtaggcg cccgacgact tgcccgcgcg cggcgcggcg
      601 tcgatccacg cgtggtcgaa gaacagttgc gcgcggcgga caaattccgg ccccatttgc
      661 gcgaaggcgc ccagcacgat gcgcttggcc tcgtcccagg tgtacgattt ctgcacatcc
      721 tcgctgagcg gcgcgttgcg gtcccagaaa tcgagcgatt tgcggcccat gatgcgggcc
      781 ttcagcgcgt aatagcggtg cgacagtctg gcgtaggact cgccgaccgc ctcggccatg
      841 gcgtcgaccg cgtccttgtc cacctcgttg gcgaggtggc gcgaggcggc ggggtcgtcg
      901 tacttgcgcc agcggtcctc gatctgcttc tcgaacgcca gcgtgttcag cgccagagcc
      961 agcaccggcg cgttctcggc cagcgccgcg ctcagcccct tggcggcggc cttgcgcgtg
     1021 gcggcctgcg gcgccgacag gcgggtcagg gcctcgttga gcgtcaggtt ttctttaccg
     1081 accctggcgc gcagccgggt cagcgtctcg tcgaacaggc gcgtccattg cgccgtcgcg
     1141 ggcattcgct ccagcatcag gcgttcgagg tcggcggaca gttcgtgcgg gcgcgaggcg
     1201 cggatgcgcc gcagccacgg gcgccagcgc tgggcgcgct ccgtggcgcg cagggcctgc
     1261 tcgatctccc agtcctccaa ctggttcagt tcgaggctca ggaacagcgc gcccgccgag
     1321 atcatcgcgg cctgagacga caggtccgac tgcatcttgg cgacggccgg gttctcgcgg
     1381 tcgatggtgc tgctgagcgt cgcataggcc agcgcgccgc ccatgtcgtc catcgccttc
     1441 tcgtacaggc cggcggcctg atcgatcagc aggccgaggc gttcggcgtt ggcgcgggcg
     1501 gaaaggaagg cgccctccag cctggccagt tccttcacat gtctttcgcc gcgcgcgaaa
     1561 tcttcccgga tgcgggggtc ttcggggccg tcgtaaaggt cggagagatc ccagatgggc
     1621 aggccggatg cggcgtcggt aggcagggtc tgtgtcatgg ccgttcagat aatccgcgcc
     1681 gacccgaaaa gcgagaggta atttaaacgc cggacccgcc cggaccggtg gcggattcgt
     1741 cgtggcgcag ggtcagcacc gccgcgccgg acgcggtgac ggcgaccgta tgttcgaatt
     1801 gcgccgacag ggagccgtcg cgcgtcacca ccgtccagcc gtcgtcttcc gttttcacat
     1861 gccgccggcc gcgattgagc atgggctcga tggtgaaggt catgcccgca tgtaaggcgg
     1921 ggccggttcc cggcttgccg aagtgcagaa tctgcggggc ctcgtgcatc tcgcggccga
     1981 tgccgtggcc gcaataatcg tgaacgacgc tatagc
//
LOCUS       DASVLL010000497         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_303096,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000497 DASVLL010000000
VERSION     DASVLL010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303096"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..784
                     /locus_tag="VF402_08295"
     CDS             17..784
                     /locus_tag="VF402_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7800336.1"
                     /translation="MTKRSVKRFMREGTLDLDQYQPDTKVRRLNVHSGGAAWSPIGHE
                     DRDQSYMKTIKPKSEGQAALLSAIDDKNLVLALGPAGTGKTYLAIAKAVEALEAGKVG
                     RIVLSRPAVEAGESIGFLPGDMEDKLAPYLRPLYDALTDRLSMKRVKALMQEGLIEIA
                     PVGYMRGRTLNNAFVVIDEAQNCTYTQLKMLLTRLGWNSTMIVTGDPNQSDLLPGLSG
                     LADVADKFEAVDAIAVVRLMDKDIVRHPLVAQMLGVL"
     gene            complement(803..>1586)
                     /locus_tag="VF402_08300"
     CDS             complement(803..>1586)
                     /locus_tag="VF402_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HEX7800337.1"
                     /translation="DFRPHEGGCGRIYVQDKGLKNDELSRLLQSLIEIGHYRKLALLG
                     FPVARDLMGWLKTAEARLNLITADMAAQNSSQDALLERLMALSAEVESRANAVRFRQG
                     ASEAYYRLTEDRLLSLRESRVEGFSTMREFIERRLQPAMRTCEAASKRLDDLATRIGR
                     ASDLLRARISISLEVQNQNLLRSMDLRARLQMKLSALVEGLSVFAVSYYVFNLVKYAL
                     DPLLSGHEQLAHRLYAPLIAAIVALAWLFITHRKKKIEGAEA"
BASE COUNT          283 a          521 c          528 g          254 t
ORIGIN      
        1 tcataaggag catcccatga ccaagcgatc cgtcaaacgc ttcatgcgcg aaggcacgct
       61 cgacctcgac cagtatcagc ccgataccaa ggtccgccgt ctgaacgtcc atagcggcgg
      121 cgcggcgtgg tcgcccatcg ggcatgagga tcgcgaccag agctacatga aaacgataaa
      181 accgaaatcg gagggccagg cggccctgct ttcggcgatc gacgacaaga atctcgtgct
      241 ggcgctcggc ccggcgggca ccggcaagac ctatctggcc atcgccaagg cggtcgaggc
      301 gctggaggcg ggcaaggtcg ggcgcatcgt gctgtcgcgc ccggcggtcg aggcgggcga
      361 atcgatcggc ttcctgcccg gcgacatgga ggacaagctg gcgccctatc tgcgcccgct
      421 ctacgacgcc ctgaccgacc ggctgtcgat gaagcgcgtc aaggcgctga tgcaggaagg
      481 gctgatcgag atcgcgccgg tcggctacat gcgcgggcgc acgctcaaca acgccttcgt
      541 ggtcatcgac gaggcgcaga actgcaccta cacgcagctc aagatgctgc tgacgcggct
      601 gggctggaac tcgaccatga tcgtgaccgg cgacccgaac cagtcggacc tgctgcccgg
      661 cctgtcggga ctggccgacg tcgccgacaa gttcgaggcg gtcgacgcca tcgcggtcgt
      721 gcgcctgatg gacaaggaca tcgtgcggca cccgctggtc gcgcagatgc tgggcgtgct
      781 gtaacgccaa tatcgaaggc gcctaggctt cggcgccttc gatcttcttc ttgcggtggg
      841 tgatgaacag ccaggccagc gcgacgatcg cggcgatcag cggcgcgtac agcctatgcg
      901 ccagttgctc atggccgctc agcagcgggt cgagcgcgta tttgacgagg ttgaacacgt
      961 agtagctgac ggcgaacacc gacaaaccct cgaccagcgc cgacagcttc atttgcaggc
     1021 gcgcgcgcag gtccatgctg cgcagcaggt tctggttctg cacctccagc gagatcgaga
     1081 tgcgcgcgcg cagcaggtcc gaggcccgcc cgatgcgcgt cgccagatcg tccagccgct
     1141 tcgaggccgc ctcgcaggtg cgcatggccg gttgcaggcg gcgctcgatg aattcgcgca
     1201 tcgtcgaaaa gccctcgacg cggctttcgc gtaaggagag cagccggtcc tcggtcaggc
     1261 ggtaataggc ttcgctggcg ccctgccgga agcgcacggc gttggcgcgg ctctcgacct
     1321 cggcggacag ggccatcagc cgctccagca gcgcgtcctg cgacgagttc tgcgccgcca
     1381 tgtcggcggt gatcaggttc agccgcgcct cggcggtctt gagccagccc atcaggtcgc
     1441 gcgccaccgg aaagcccagc agggccagtt tgcggtaatg gccgatctcg atcaggctct
     1501 gcaacaggcg cgacaactcg tcgttcttca ggcccttgtc ctgcacgtag atgcgcccgc
     1561 agccgccttc gtgcgggcgg aaatcg
//
LOCUS       DASVLL010000498         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_87359, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000498 DASVLL010000000
VERSION     DASVLL010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87359"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1252)
                     /locus_tag="VF402_08305"
     CDS             complement(32..1252)
                     /locus_tag="VF402_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800338.1"
                     /translation="MTAQVSEPTAPSTTKTGSGIVLSVILAVSFCHLLNDMMQSLLPA
                     IYPNLQARFGLSFAQIGLVTLAYQITASLLQPLVGLYADRRPTPLALPGGTLFTLAGL
                     SVLSVAHSYGVLLIGACLLGVGSSVFHPESSRVVRMAAGGRHGLGQSLFQVGGNAGSA
                     LGPLAAAIVVVRWGQSSLALFALLALLSCAVLWFVSRWYRHHGLERIRRAHKSETAAA
                     PSSKTALGGVAILLGLIFSKYVYLASITSYFTFYLIERFHVSVEVAQMHLFVFLAAVA
                     VGTVAGGPLGDRFGRKYVIWFSILGALPFTLLLPHASLFWTGPLSVIIGLILASAFPA
                     IVVFAQELMPGKVGMISGLFFGFSFGMGGLGAAALGWLADRTGIEAVYAVCAVLPAIG
                     LIAAFLPNLKKSGG"
     gene            1358..>1554
                     /locus_tag="VF402_08310"
     CDS             1358..>1554
                     /locus_tag="VF402_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800339.1"
                     /translation="MRNVDPGVYEDTPRPVIAIGNDYPPAFELARHTHRRAQLLYPAE
                     GVVTVDTAHGAWVVPPERAVW"
BASE COUNT          308 a          520 c          519 g          207 t
ORIGIN      
        1 cctgatccgc gtgaccgcgc ccgaggacac cttagccgcc tgattttttc aggttcggca
       61 ggaaggccgc gatcaggccg atggccggca ggacggcgca gacggcgtac accgcctcta
      121 tgcccgtccg gtcggccagc cagcccagcg ccgctgcgcc cagaccgccc atgccgaacg
      181 agaagccgaa gaacaggccg gagatcatgc cgaccttgcc cggcatcagc tcctgcgcga
      241 acacgacgat ggccgggaag gccgaggcca ggatcaggcc gatgatcacg ctgagcggac
      301 cggtccagaa caggctggcg tgcggcagca acaacgtgaa cggcagggcg cccaggatcg
      361 agaaccagat cacgtacttg cgcccgaagc ggtcgcccag cggcccgccc gccaccgtgc
      421 cgaccgcgac ggcggccagg aacacgaaca ggtgcatctg cgcgacctcg accgagacat
      481 ggaagcgctc gatcaggtag aaggtgaagt agctggtgat gctggccaga tagacgtatt
      541 tcgagaagat caggcccagc aggatggcga cgccgcccag agcggtcttc gacgacggcg
      601 ccgccgccgt ttcggattta tgcgcgcggc ggatgcgctc aagaccgtga tgcctgtacc
      661 agcggctgac gaaccacagc accgcgcacg acaaaagcgc cagcagcgcg aacagcgcca
      721 ggctcgactg tccccagcgc accaccacga tggcggcggc cagcggcccc agcgccgacc
      781 cggcgttgcc gccgacctga aacagcgact gccccagacc atgtcgcccg cccgccgcca
      841 tccgcaccac gcgcgacgat tccgggtgaa agaccgacga gccgacgccc agcaggcacg
      901 ccccgatcag cagcacgccg taactgtgcg cgaccgacag gacgctcagc cccgccagcg
      961 tgaacagggt gccgccgggc agggccagcg gcgtcggccg ccggtcggcg tacagcccga
     1021 ccaggggctg caacagcgaa gcggtgatct gataggccag ggtcacgagg ccgatctgcg
     1081 cgaagctgag gccgaaacgc gcctgaaggt tcggatagat ggcgggcagg agcgactgca
     1141 tcatgtcgtt gagcaggtgg cagaagctga ccgccagaat gaccgacagg acgatgcctg
     1201 agccggtctt ggtcgtgctg ggggcggtag gttccgagac ctgagcggtc atgacgctga
     1261 ctccttgact gtcataaagc gagctgctgt ttaggcgctt ttcgtgtgtc ccgcattcct
     1321 atatgggata atcattttcg caaatgggac aatcgccatg cgcaatgtcg atccgggcgt
     1381 ctatgaggac acgccgcgtc cggtcatcgc catcggcaac gactatccgc ccgccttcga
     1441 gctggcgcgg cacacgcacc ggcgcgcgca actgctctat ccggcggaag gggtggtgac
     1501 ggtcgacacc gcgcacggcg cctgggtcgt gccgccggag cgcgcggtgt ggat
//
LOCUS       DASVLL010000499         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_87375, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000499 DASVLL010000000
VERSION     DASVLL010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87375"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF402_08315"
     CDS             <1..180
                     /locus_tag="VF402_08315"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEX7800340.1"
                     /translation="PAGPDTPHYEMIDAAPCVYNRLVVYRSISLHAGLLGASTLTDDP
                     RTGRLTLNTLIQLRG"
     gene            complement(376..990)
                     /gene="grpE"
                     /locus_tag="VF402_08320"
     CDS             complement(376..990)
                     /gene="grpE"
                     /locus_tag="VF402_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7800341.1"
                     /translation="MSDTPETEGANLDQDAPINKDGALLNAELEKLTAENAALKEQAL
                     RYAADAENTKRRAEREMNDARAFAIQRFARDLLGVADILQRALGAAPSDLVDPAFKNF
                     VAGIDMTEKELAGAFERNGVKKIAPLKGEKFDPHRHQAVSEIPSDEVEGGGVVTVMQT
                     GYELFGRVIRPAMVAVAARKTPAQPNGYAPEAPEDTGENIDTKA"
     gene            complement(1027..2100)
                     /gene="hrcA"
                     /locus_tag="VF402_08325"
     CDS             complement(1027..2100)
                     /gene="hrcA"
                     /locus_tag="VF402_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7800342.1"
                     /translation="MSLLNSTASLSELDARARDIFRHVVETYLETGEPVGSRTVSKSG
                     VHLSPASIRNTMQDLAHLGLLSAPHTSSGRLPTHQGLRLFVDGLLEIGDLPEDDKRDI
                     EARLTGKGQTFDQALHEASNILSGLAGGAGIVMTPAFEAGVRHVEFVALGPDQALAVL
                     VYADGRVENRLMALQAGMTPAVLIEASNYLNTHLRGRTLTEARASLKAELEAERRRLD
                     EAAATLIETGLAVWSGGDAGQRALIVRGRANLLGAGQSAEDLERVRSLFEDLEQKEEL
                     IDLLDDVAGAQGVRIFIGAESKLFSLSGSAVIAAPYMVQQPKTGAGRVVGAIGVIGPA
                     RLNYARIIPLVDYTARILGRLLD"
BASE COUNT          338 a          742 c          743 g          386 t
ORIGIN      
        1 cccgccgggc cggacacgcc gcattacgag atgatcgacg ccgcgccgtg cgtctataac
       61 cggctggtcg tttatcgcag catcagcctg catgccgggc tgctgggcgc ctcgaccctg
      121 acggacgatc cgcgcaccgg ccggttgacg ctcaacaccc tgatccagtt gcgcggataa
      181 ggccgaccta cccctctccc ctccacgagg ggagaggacg agagccgaac gaagtgaagg
      241 cgatcgggtg aggggccgcc agccggttgc gctccgtcgg cgggcggccc ctcacccgag
      301 gatcggcggc tgcgccttgc tcctcgtcct ctccccattg tcatggggag aggggaaggg
      361 aggggtgtaa ggccgttacg ccttggtgtc gatgttttcg ccggtgtctt ccggcgcttc
      421 gggggcgtag ccgttgggtt gcgcgggggt cttgcgcgcg gccacggcga ccatggccgg
      481 gcggatgacg cggccgaaca gctcgtagcc ggtctgcatg acggtgacga cgccgccgcc
      541 ctcgacctcg tcggacggga tttcgctgac cgcctgatgc cggtgcgggt cgaacttctc
      601 gcctttcagc ggcgcgatct ttttcacgcc gttgcgctcg aacgccccgg ccagctcttt
      661 ttcggtcatg tcgatgccgg cgacgaagtt cttgaacgcc ggatcgacga ggtccgaggg
      721 cgccgcgccc agcgcgcgtt gcaggatgtc ggccacgccc aaaaggtcgc gggcgaagcg
      781 ctggatggcg aaggcgcgcg cgtcgttcat ctcgcgctcg gcgcggcgct tggtgttttc
      841 ggcgtcggcg gcgtagcgca gggcctgttc cttcaaggcc gcgttctccg ccgtcaactt
      901 ctccagctcg gcgttgagca gggcgccatc tttatttatg ggcgcgtcct gatcgaggtt
      961 ggcgccttcg gtttcgggcg tatcgctcat ggtcttgact gatccttcaa atatcgccgg
     1021 aggccgctaa tcgagcaggc ggcccagaat ccgggcggtg tagtccacaa gtgggatgat
     1081 gcgcgcataa ttcaaccggg ccgggccgat cacgccaatg gcgccgacca ccctgcccgc
     1141 gccggtcttg ggctgctgca ccatgtaggg cgctgcaata acagccgagc cggacaaaga
     1201 aaacagtttc gactccgcgc cgatgaaaat tctcaccccc tgcgcgcccg cgacgtcgtc
     1261 caaaaggtcg atcagctcct ccttctgctc cagatcctcg aacagggagc gcacccgttc
     1321 gaggtcttcg gcgctttgcc cggcgcccag aaggttggcg cggccgcgca cgatcagggc
     1381 gcgctggccc gcgtcgccgc ccgaccagac cgcaagcccc gtctcgatca gggtcgccgc
     1441 cgcctcgtcc agccgccgcc gttcggcttc cagctcggcc ttcaggctgg cgcgcgcctc
     1501 ggtcagggtg cggccgcgca gatgggtgtt gagatagttg gacgcctcga tcagcacggc
     1561 gggcgtcatc ccggcctgca gggccatcag ccggttctcc acccggccgt cggcatagac
     1621 cagcacggcc agcgcctgat cggggccgag ggcgacgaat tcgacgtggc gcaccccggc
     1681 ctcgaaggcg ggcgtcatca cgattccggc gccgcccgcc agaccggaca ggatgttgct
     1741 ggcctcgtgc agggcctgat cgaaggtctg gcccttgccg gtcaggcgcg cctcgatgtc
     1801 gcgcttgtcg tcctcgggca ggtcgccgat ctccaaaagc ccgtccacga acaggcgcag
     1861 gccctgatgc gtcggcagcc gccccgacga ggtgtgcggc gcggacagaa gccccagatg
     1921 cgccagatcc tgcatggtgt tgcggatcga ggcgggcgac aggtgaacgc cgcttttcga
     1981 caccgtgcgc gacccgaccg gctcgccggt ttccagatag gtctcgacga catggcggaa
     2041 gatgtcgcgc gcccgcgcgt ccagctcgct cagcgaagcc gtcgaattga gcagactcat
     2101 cgaatagggc gaatgcgaca tggccgccag cttagcaaac cgggccgcgg cgcgacaagt
     2161 atttagggct ttgcagcaca ggttaacggg tatagcctcc cggttcttg
//
LOCUS       DASVLL010000500         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_464629,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000500 DASVLL010000000
VERSION     DASVLL010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464629"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VF402_08330"
     CDS             <1..168
                     /locus_tag="VF402_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEX7800343.1"
                     /translation="IISLSLWVNRHTSLVMRNKEEIGPLIDINRTIMQGLLPQRQAAG
                     ETPVMARQSYG"
     gene            complement(188..1126)
                     /locus_tag="VF402_08335"
     CDS             complement(188..1126)
                     /locus_tag="VF402_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7800344.1"
                     /translation="MSAAAILSGLLPVFLLIALGYGLRKSKFLAETAWRPIEIFAVYV
                     LYPGFLVPAIWHADLSGPSAGPVGAAILITIAISAGLGLALKPVLRLSGPTYSSVFQG
                     LIRFNSFVFIPAATAIFGPKAMSLAAVAVSALIPLTNLLSILALAHWGEPEGGGGERP
                     PLHLLRVIISNPIFAACLLGLALNLIRAPGVPPLEKALTMLGEAAIPTGLILAGAGLS
                     FGYVARQPWLTAGTAIYKTLIMPVICWGICRALGGDSLAQGVALCCGAAPSAAAAYVL
                     ARHMGGDAPLMAGVIALTTILSAAAMPLLLIAFHLG"
     gene            complement(1123..1596)
                     /gene="ruvX"
                     /locus_tag="VF402_08340"
     CDS             complement(1123..1596)
                     /gene="ruvX"
                     /locus_tag="VF402_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7800345.1"
                     /translation="MPVLDITDLKTALPPRRALLGLDLGEKTIGVAVSDISLTLASPL
                     ELIRKSKFTQEAERLFHLMAQREADGMVIGLPVNMDGSEGPRCQSVRAFGRNLLRLRD
                     LPIAFWDERWSSSVMNRFLIEAADLSRAKRAEVIDRNAAAYILQGALDRMRDAAP"
     gene            1849..2205
                     /locus_tag="VF402_08345"
     CDS             1849..2205
                     /locus_tag="VF402_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEX7800346.1"
                     /translation="MLRTVLALAAAASALTFVAGSAQAQTRTPDQVTISTRHVDFNDA
                     GQMRALYAKLQFAARSVCESEITSAEIARADKACARQALSDAVRRIGAPQLSRLDADE
                     NKRDADGTQVLASLDR"
     gene            2344..2697
                     /locus_tag="VF402_08350"
     CDS             2344..2697
                     /locus_tag="VF402_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEX7800347.1"
                     /translation="MASALAAGALGTACACEAHDWARDDATTRVAVTFRTTDLSDPAR
                     TPDVRARLFAAVQRACQSEGEGPKWREADDRACEAEAMRDAEQRLRAARPDASAPEAL
                     IREDATQTRRLFAGR"
BASE COUNT          513 a          920 c          886 g          423 t
ORIGIN      
        1 atcatctcgc tgtcgctctg ggtgaaccgg cacacctcgc tggtcatgcg caacaaggaa
       61 gagatcgggc cgctgatcga catcaaccgc accatcatgc agggcctgct gccccagcgc
      121 caggcggcgg gcgagacgcc cgtcatggcg cgtcaatcct acggttaggg cttacggtta
      181 aaacgcgcta ccccagatgg aacgcgatga gcagcagcgg catggccgcc gcgctcagga
      241 tcgtggtcag ggcgatcacg cccgccatca gcggcgcgtc gccgcccatg tgccgggcca
      301 gcacataggc cgccgccgcc gacggcgcgg cgccgcagca cagggccacg ccctgcgcca
      361 gcgaatcgcc gcccagcgcg cggcatatcc cccagcagat caccggcatg atcagcgtct
      421 tgtagatcgc cgtccccgcg gtcagccacg gctggcgcgc gacatagccg aagctgaggc
      481 ccgccccggc caggatcagc ccggtcggaa tcgccgcctc gcccagcatg gtcagcgcct
      541 tctccagcgg cggaacgcct ggcgcgcgta tgagattcag cgccagcccc agcaggcagg
      601 cggcgaagat cggattgctg atgatgacgc gcagcagatg cagcggcggg cgttcgccgc
      661 cgccgccttc gggttcgccc cagtgcgcga gggcgagaat gctcaacagg ttggtgagcg
      721 ggatcagcgc gctgaccgcc acggcggcca ggctcatcgc cttcgggccg aagatcgccg
      781 tcgccgccgg gatgaagacg aacgagttga agcggatcag cccctgaaac acgctggaat
      841 aggtcgggcc gctcagccgc aggaccggct tcagcgccag gcccaggccc gccgagatcg
      901 cgatggtgat caggatcgcc gccccgaccg gcccggcgga cggccccgac aggtcggcgt
      961 gccagatggc cgggacgaga aagcccggat acagcacata gacggcgaag atttcgatcg
     1021 gccgccaggc cgtttcggcc agaaatttcg atttgcgcag gccgtagccg agcgcgatga
     1081 gcagaaagac cggcagcagc ccgctcagga tcgccgccgc gctcacggcg ccgcgtcccg
     1141 catccggtcc agcgcgcctt gcaggatata ggcggcggcg ttgcggtcga tgacctcggc
     1201 gcgtttggcg cgcgacagat cggccgcctc gatcaggaag cggttcatca ccgacgacga
     1261 ccagcgctcg tcccagaagg cgatcggcag gtcgcgcagg cgaagaagat tgcgcccgaa
     1321 cgcgcgcacg gactggcagc gcgggccttc gcttccgtcc atattgaccg gcaggccgat
     1381 gaccatgccg tcggcctcgc gctgcgccat caggtggaac aggcgctcgg cctcctgcgt
     1441 gaacttgctc ttgcggatca gctccagcgg cgaggcgagg gtcagggaaa tatcggacac
     1501 ggccacgccg atggtctttt cgccgaggtc gagccccagc agggcgcggc gcgggggcag
     1561 ggcggttttc aggtcggtga tgtcgagtac gggcatggct tccccagtgg cgccgccgcc
     1621 gccttgagtc aatgcgcgcc gccgccgggt cgtcgcaaat gcgaatatat cgattgcaac
     1681 ataagtatta aatttatgtc atacattaac atcctgtaat ttatgaaacc catgtaatac
     1741 atgaaaccga tttaacaata ttgatgtttg aattgacgca aagcacgtgt atttgttgcc
     1801 tcagttgatt ttctttatct ttagaaactg tatctcagga aacatatcat gcttcgcacc
     1861 gttctcgcgc tcgccgccgc cgcttccgcc ctgaccttcg tcgccggctc ggcgcaggcg
     1921 caaacccgca ctcccgatca ggtcaccatc tcgacgcgcc atgtcgattt caacgacgcg
     1981 ggccagatgc gcgcccttta cgccaaactg caattcgcgg ccaggtcggt ctgcgaaagc
     2041 gagatcacca gcgccgagat cgccagggcc gataaggcct gcgcgcgtca ggcgctgagc
     2101 gacgccgtcc gccggatcgg cgcgccgcaa ctgagccgcc tcgacgcgga tgaaaacaag
     2161 cgcgacgccg acgggacgca ggtcctggcc tcgctcgacc gctaaagcgc tttccgaaaa
     2221 gtgtgacgca cttttcggat taaaaagcgc gtaaaagcat aaagttagag cgccgatctg
     2281 atgcaatcag atcggaaaac gctctaagcc ccggagtctc tcatgaaaat cctgtcgctt
     2341 gttatggcgt cggccttggc cgccggcgcc cttgggaccg cttgcgcctg tgaggcgcat
     2401 gactgggcgc gcgatgacgc gacgacgcgc gtggcggtca cgttccgcac tacggacctg
     2461 agcgatccgg ccagaacgcc cgacgtgcgc gcccgcctgt tcgcggcggt ccagcgcgcc
     2521 tgccaaagcg aaggcgaggg gccgaaatgg cgcgaagccg acgaccgcgc ctgcgaggcc
     2581 gaggccatgc gggacgcgga gcagcggttg cgcgcggcgc ggcccgacgc ttcggcccct
     2641 gaagctctga tccgcgagga cgcgacgcaa acccggcgcc tgttcgccgg ccgctgacgg
     2701 cctgtctccc tgtccgccag ccctcaaaga ccgcgcggcg cg
//
LOCUS       DASVLL010000501         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_355628,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000501 DASVLL010000000
VERSION     DASVLL010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355628"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF402_08355"
     CDS             complement(<1..294)
                     /locus_tag="VF402_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7800348.1"
                     /translation="MRFLHAMIRVHDLDAALRFFRDGLGLRQVRRHDSEQGRYSLIFL
                     SADEDAHLGAKDGLRGQAEVELTYNWPDGNGVVETYAGGRNFGHLAYEVDDIYA"
     gene            complement(304..522)
                     /locus_tag="VF402_08360"
     CDS             complement(304..522)
                     /locus_tag="VF402_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX7800349.1"
                     /translation="MSDALIPPPEVIYVDTHKVACNGGGGALGHPLTYYELGEDGVVE
                     CGYCDRKFVHKDHAHGYHDPAPRPEGDH"
     gene            638..1936
                     /locus_tag="VF402_08365"
     CDS             638..1936
                     /locus_tag="VF402_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800350.1"
                     /translation="MNRDQQGGRRRLTSIFGGAMGNLVEWYDWYAYSAFALYFSKAFF
                     PHGDRTAQLLNTAAIFAVGFFMRPVGGWLLGWWGDRYGRRAALTLSVLLMCFGSLIIA
                     CAPPFAAIGVASPLLLVLARLMQGLSVGGEYGASAAYLSEMAGRRHRGFWSSFQYVTL
                     IMGQLIALALLIVLQQWLLTPAQLEAWGWRIPFVVGALLALAALFIRRHIDETPAFRH
                     SAAVAGSRTLHLLRHYPLAFLQVLGLTLGGTLAFYTYTTYMQKFLVNTAGWTKPRATL
                     LSALTLIVYAALQPAFGALSDRIGRKPLLVGFGAAGVIFTVPIMTALSHAQSFWTAFA
                     LVMAALVIVSGYTSINAVVKAELFPAHVRTLGVALPYALAVSLFGGSAEYVALWLKQS
                     GHETRFFAYVAACIGVSLLVYVFTADTQKTSAFHADEDAS"
     regulatory      1970..2079
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2152..3132
                     /locus_tag="VF402_08370"
     CDS             2152..3132
                     /locus_tag="VF402_08370"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEX7800351.1"
                     /translation="MNALTPNAPDLTGTDITAVLPADFRLQSGQRLEQTEVTARLYGP
                     RDGPVVVAAGGISAGRVLFAKEDSTETAPGWWEGVAGPGEALDLNRFRVLSIEFAPRL
                     PASRVYTISTHDQARLVKLLLDRIGVARIYSFIGASYGAMIALAFAELYPDAVERLCV
                     ISAAHRAHPMATAFRGLQRRLLQFGAENGRPREGISLARQLAMTTYRSDAEFAERFDG
                     PPPGEAGGDYTVCGYLRARGDAYAATDVNRWISMSDSLDRHRVDPAKIKARVFLAAVP
                     IDRLVPYDDMKLLHDQLADSVFIDFPSLYGHDAFLKEIKLVSDIVRTSLA"
     gene            3139..4308
                     /gene="metB"
                     /locus_tag="VF402_08375"
     CDS             3139..4308
                     /gene="metB"
                     /locus_tag="VF402_08375"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX7800352.1"
                     /translation="MTKDYLKDAAPATKAVNIGVDSDPAHGAVMPPIYLTTNYSFAGF
                     GEKRGYDYARSGNPTRDVIAEVIAELEGGAGAVMTASGLAALDLLWQDLPGNRRVVVP
                     HDCYGGTQRLLNARHKQGLIDLVYVDQGDDAALKKALSEPVALLLLETPSNPLMRLID
                     LEKLIALGHKAGAKVAVDNTFLSPALQNPIAFGADFVVHSTTKYINGHSDVIGGALVC
                     TTEADVTAYRWWANCLGSTPAAFDSYMTLRGVRTLMVRLRQQQESAQAIAEYLEAHPA
                     VKVVHYPGLASHPQHELAKRQQKGFGAMMSFELAGGLDAVRTLLADIDLFSLAESLGG
                     VESLIVHPGSMTHAAMSPQAQATAGITEGLLRLSIGLEDKDDLIKALSRALDRVA"
BASE COUNT          707 a         1492 c         1475 g          790 t
ORIGIN      
        1 ggcgtagatg tcgtcgacct cataggcgag atggccgaaa ttgcgtccgc ccgcgtaagt
       61 ctcgaccacg ccgttgccgt ccggccagtt gtacgtcagc tcgacctcgg cctgaccgcg
      121 caaaccgtct ttcgcgccca gatgcgcatc ctcgtccgcc gacaggaaga tcaggctgta
      181 gcgcccctgt tcgctgtcgt ggcggcggac ctgacgcagg cccaggccgt cgcggaagaa
      241 gcgcagggcc gcgtcgagat cgtggacgcg gatcatggcg tgcaggaagc gcatcgggcg
      301 gacttagtga tcgccttcgg gacgcggcgc cgggtcgtga tagccgtgcg catggtcctt
      361 atgcacgaac ttgcggtcgc aatagccgca ttcgaccacg ccgtcctcgc ccagctcgta
      421 ataggtcagc ggatggccca gcgcgccgcc gccgccgttg caggcgacct tgtgcgtatc
      481 gacataaatc acttcggggg gcggaatcag ggcatcgctc atggcggcgc aaacctctac
      541 ttgaaaacgt ccgcttctcc ttaatcgaca acgcccggcc cggcaagacg ggccgcgtcg
      601 atttgcgctt tgccgcattt cgtgaaatcg tcggcgcatg aacagggatc aacaaggcgg
      661 gcgccgacgg ctgacctcca tcttcggcgg cgcgatgggc aatctggtcg aatggtacga
      721 ctggtacgcc tattcggcct tcgcgctcta tttctccaaa gccttctttc cgcacggcga
      781 ccgcaccgcg cagttgctca acacggcggc gatcttcgcg gtcggcttct tcatgcggcc
      841 ggtcggcggc tggctgctgg gctggtgggg cgaccgctac gggcggcggg cggcgctgac
      901 cctgtcggtg ctgctgatgt gcttcggctc gctgatcatc gcctgcgcgc cgccgttcgc
      961 cgccatcggc gtcgcctcgc cgctgctgct ggtgctggcg cggctgatgc agggcttgag
     1021 cgtcggcggc gaatacggcg ccagcgccgc ctatctgtcg gaaatggccg gccgcaggca
     1081 tcgcggcttc tggtccagct ttcagtatgt gacgctgatc atggggcaac tgatcgcgct
     1141 ggccctgctg atcgtgttgc agcaatggct gctgacgccc gcgcagcttg aggcgtgggg
     1201 ctggcggatt ccgttcgtcg tcggggcctt gctggcgctg gccgccctgt tcatccgccg
     1261 ccatatcgac gagacgcccg ccttccgcca cagcgcggcg gtcgcgggca gccgcaccct
     1321 gcacctgctg cgccattatc cgctggcgtt cctgcaggtg ctgggcctga cgctgggcgg
     1381 cacgctggcc ttctacacct ataccaccta catgcagaaa ttcctcgtca acacggcggg
     1441 ctggacgaag ccgcgcgcga cgcttctgtc ggccctgacc ctgatcgtct atgcggccct
     1501 gcaaccggct ttcggcgccc tgtccgaccg catcgggcgc aagcccttgc tggtcggctt
     1561 cggcgcggcg ggcgtgatct tcaccgttcc gatcatgacc gccctgtcgc acgcgcaatc
     1621 gttctggacc gcgttcgcgc tggtgatggc ggcgctggtc atcgtgtcgg gctatacgtc
     1681 gatcaacgcc gtggtcaagg ccgagctgtt ccccgcccat gtgcgcacct taggcgtggc
     1741 cctgccctat gcgctggccg tgtccctgtt cggcggctcg gcggaatatg tcgcgctgtg
     1801 gctgaagcag tccgggcatg agacgcgctt cttcgcctac gtcgccgcct gcatcggcgt
     1861 gtcgctgctg gtttacgtct tcaccgccga cacgcagaag acctcggcct tccacgccga
     1921 cgaggacgcc tcttgacggc ggcgcggact tgcgcgcata agcatgcaga actcatcgag
     1981 accagccgag ggaaaggccc gacgacgctg gggcaaccgc tgccgacaag gcggaatggt
     2041 gccaactcct gcgcagccca tttctgcgaa agatgagaga cgcgaacgtc cgggcgcagg
     2101 cgcccgtcgg tctctcgact ggtgtcgatg cttagcttga gaccgccaga gatgaacgcc
     2161 cttacgccca acgcccccga cctgaccgga accgatatca ccgccgtcct acccgccgat
     2221 ttccgcctgc aaagcgggca gaggctggag cagaccgagg tgacggcgcg gctctatggc
     2281 cccagggacg gccccgtggt cgtggcggcg ggcggcatct ccgccgggcg cgtgctgttc
     2341 gccaaggagg attcgaccga gacggcgccc ggctggtggg aaggcgtggc cggtcccggc
     2401 gaggcgctgg acctcaaccg gttccgcgtg ctgtccatcg agttcgcgcc gcgcctgccc
     2461 gcgtcgcgcg tctataccat ctcgacccac gatcaggcgc ggctggtcaa gctgctgctg
     2521 gaccggatcg gggttgcgag gatctacagc tttatcggcg cctcctacgg cgccatgatc
     2581 gcgctggcct tcgccgagct ttatcccgac gcggtcgagc gtttgtgcgt gatctcggcg
     2641 gcgcaccgcg cgcacccgat ggcgacggcg ttccgcgggc tgcaacggcg gctgctgcaa
     2701 ttcggcgccg agaacggccg gccgcgggaa ggcatttcgc tggcgcggca actggccatg
     2761 accacctatc gctccgacgc cgagttcgcc gagcgcttcg acgggccgcc gcccggcgag
     2821 gccgggggcg attacacggt ctgcggctat ctgcgcgcgc gcggcgacgc ctatgcggcc
     2881 accgacgtca accgctggat ctcgatgtcg gacagcctcg accgccaccg cgtcgatccg
     2941 gcgaagatca aggccagggt gttcctggcc gccgtgccca tcgaccggct ggtgccttac
     3001 gacgacatga agctgctgca cgaccaactg gccgacagcg tgttcatcga cttcccctcg
     3061 ctctacggac acgacgcctt cctgaaggaa atcaagctcg tgtccgatat cgtccgcacc
     3121 tcgttagcgt aaagacccat gaccaaggac tatctcaaag acgccgcgcc cgccaccaag
     3181 gccgtcaata tcggcgtcga ttccgacccg gcgcacggcg cggtgatgcc gccgatctat
     3241 ctgaccacca actattcgtt cgcgggcttc ggcgaaaagc gcggctacga ctatgcgcgc
     3301 tccggcaatc cgacgcgcga cgtgatcgcc gaggtgatcg ccgaactgga aggcggcgcc
     3361 ggggcggtga tgaccgcgtc gggtctggcg gcgctcgacc tgctctggca ggacttgccg
     3421 ggaaaccggc gcgtggtggt tccgcacgac tgctacggcg gcacgcagcg ccttttgaac
     3481 gcgcgccata aacaggggct gatcgacctc gtttacgtcg atcagggcga cgacgcggcg
     3541 ctgaaaaagg cgctgtcgga accggtcgcg ctgctcttgc tggagacgcc gtccaatccg
     3601 ctgatgcggc tgatcgacct tgaaaagctg atcgcgctcg gccacaaggc gggggcgaag
     3661 gtcgcggtcg acaacacgtt tttatcgccc gcgctgcaaa atccgatcgc cttcggggcg
     3721 gacttcgtcg tgcattcgac gaccaagtac atcaacggcc attccgacgt gatcggcggc
     3781 gcgctggtct gcacgaccga ggcggacgtg acggcgtatc gctggtgggc caactgcctc
     3841 ggctcgaccc cggcggcgtt cgattcctac atgacgctgc gcggcgtgcg cacgctcatg
     3901 gtgcgcctgc gccagcagca ggagagcgcg caggccatcg ccgaatatct cgaagcgcat
     3961 ccggcggtga aggtcgtgca ttatccgggc ctcgcctcgc acccgcagca cgaactggcc
     4021 aaacgccagc aaaagggctt cggcgccatg atgagtttcg agcttgccgg cggcctcgac
     4081 gcggtcagga cgctgctggc cgacatcgac ctgttcagcc tggccgaatc gctgggcggg
     4141 gtcgagagcc tgatcgtgca tccgggcagc atgacgcacg cggccatgtc gccgcaagcg
     4201 caggcgacgg cgggcatcac cgaagggctg ttgcgcctgt cgatcgggct cgaggacaag
     4261 gacgatctga tcaaagcgct gagccgcgcg ctggaccgcg tggcttaggt tggatcgaca
     4321 ttcacttggc ttgagcggat cgcgctttgg cggaacgcgg acgtatccgc ggatggctca
     4381 gatcaaggaa gggatttaac cacggacgat ggccttcggc catcttcacg gaccgccgct
     4441 tgcgcggcgt acacggacaa aggg
//
LOCUS       DASVLL010000502         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_464754,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000502 DASVLL010000000
VERSION     DASVLL010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464754"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VF402_08380"
     CDS             complement(<1..715)
                     /locus_tag="VF402_08380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723422.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7800353.1"
                     /translation="MQLNPAEISELIKSRIEGLAASADIRNQGTVISVTDGIVRVHGL
                     SDAMAGEMLEFKPTPDGTPTFGLALNLERDSVGAVILGEYEHISEGDTVKCTGRILEV
                     PVGPELIGRVVNALGQPIDGKGPINARLTDVIEKVAPGVIARKSVDQPVQTGLKAIDS
                     MVPIGRGQRELIIGDRQTGKSAVAVDTIINQKGQNMTCVYVAIGQKASTIKNLVRALE
                     ANGAMEYTIVVAATASESAA"
     gene            complement(752..1282)
                     /locus_tag="VF402_08385"
     CDS             complement(752..1282)
                     /locus_tag="VF402_08385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX7800354.1"
                     /translation="MAELATIARPYAEALFEASRGDLGGTSQWLDALAAVAGNEQLLQ
                     FADNPKTGEQQVFDVVVDVARVQLPEPGRNFLRMVIDNGRLAALPEIAHQFRAMKNAQ
                     SGSSDAVVYSAFPIAADQLGPVAQALERRFGRKLNVAVQEDPSLIGGIRVVVGDEVLD
                     TSVKARLEQMKVALTA"
     gene            complement(1298..1768)
                     /locus_tag="VF402_08390"
     CDS             complement(1298..1768)
                     /locus_tag="VF402_08390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX7800355.1"
                     /translation="MSITGTLIIQIIVFLILVGFTMKFVWPPIAVALDERAKKIAEGL
                     AAADKAKADLAAADRRVEEELAKSRSETAALLADAERRAQAIVEEAKGRAAEEGAKII
                     AAARAEAEQQAVKAREALREQVAALAVKGAEQILRKEVNAGVHAELLKRLQTEL"
     gene            complement(1834..2097)
                     /gene="atpE"
                     /locus_tag="VF402_08395"
     CDS             complement(1834..2097)
                     /gene="atpE"
                     /locus_tag="VF402_08395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX7800356.1"
                     /translation="MEVISFVALAAGLIIGLGAIGACIGIGVMGSKYLESAARQPELM
                     NELQTKMFLLAGLIDAAFIIGTGIALWFATANPFLGQIANLPK"
     gene            complement(2172..>2860)
                     /gene="atpB"
                     /locus_tag="VF402_08400"
     CDS             complement(2172..>2860)
                     /gene="atpB"
                     /locus_tag="VF402_08400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004477.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7800357.1"
                     /translation="RRATAGVPGRFQAAVEILVEMVEAQAKGIVHNATSRKFVSPLAL
                     TIFIWIILMNTMDLLPVDLIPAIWKGIYGATGHDPEHAYMRVVPTADLSISMGLSISV
                     LLICLFYNVKIKGVGGWVHELFTAPFGPHPLLWPFNFAMQMIEFVAKTVSHGMRLFGN
                     MYAGELIFLLIAMMGGAFSFTGTGIGLAIGHIIAGSAWAIFHILIIVLQAFVFMMLTL
                     VYVGQAHDAH"
BASE COUNT          454 a          911 c          990 g          505 t
ORIGIN      
        1 tggcggcgga ttcggaagcc gtggcggcca cgacgatggt gtattccatc gcgccgttcg
       61 cctcgagcgc gcgcaccagg ttcttgatcg tggacgcctt ctgcccgatg gcgacgtaca
      121 cgcaggtcat gttctgaccc ttctggttga tgatcgtgtc gaccgcgacg gcggacttgc
      181 cggtctggcg gtcgccgatg atcagttcgc gctggccgcg gccgatcggc accatcgagt
      241 cgatggcctt caggccggtc tgcaccggct ggtccaccga cttgcgcgcg atcacgcccg
      301 gggcgacctt ctcgatcacg tccgtcaggc gggcgttgat cgggcccttg ccgtcgatcg
      361 gctggcccag cgcgttcacc acgcggccga tcagttcggg gccgacgggc acttcgagaa
      421 tgcggcccgt gcacttgacg gtgtcgcctt cggagatgtg ctcgtactcg cccaggatca
      481 ccgcgccgac cgagtcgcgc tcgaggttca gcgcgaggcc gaacgtgggc gtgccgtcgg
      541 gcgtgggctt gaactccagc atttcgccgg ccatcgcgtc ggacaggccg tggacgcgga
      601 cgatgccgtc ggtcacggag atcaccgtgc cctggttgcg gatgtcggcg ctggcggcca
      661 ggccttcgat ccggctcttg atcagttcgc taatttctgc gggattgagc tgcatgactc
      721 gttccttttg tccttggagc ggaaacgacc ctcaggccgt cagcgcgact ttcatttgtt
      781 ccaggcgggc cttcaccgag gtgtccagca cctcgtcgcc gaccaccacg cgaatgccgc
      841 cgatgaggga cgggtcttcc tgcacggcca cgttcagctt gcggccgaag cggcgttcca
      901 gcgcctgcgc caccgggccg agctggtcgg ccgcgatcgg gaaggcgctg tagaccacgg
      961 cgtcggacga gccgctctgg gcgttcttca tcgcgcggaa ctggtgcgcg atctcgggca
     1021 gcgccgcgag gcggccgttg tcgatcacca tgcgcaggaa gttgcggccc ggctcgggca
     1081 gctgcacgcg ggcaacgtcg acaaccacgt cgaaaacctg ctgctcgccg gtcttcggat
     1141 tgtccgcgaa ctgcagcagc tgctcattgc ccgcgacggc agcgagggcg tcgagccact
     1201 gcgaggtgcc gcccaggtca ccccgggacg cctcgaacag cgcctcggcg tacgggcgcg
     1261 cgatggtggc gagttcagcc atggccttcg ttccttctta cagctcggtc tgcaggcgct
     1321 tgagcagctc ggcgtggacg ccggcgttca cttccttgcg caggatctgc tccgcaccct
     1381 tcacggccag cgcggcgacc tgctcgcgca gggcctcgcg ggccttgacg gcctgctgct
     1441 cggcctcggc acgggcggcg gcgatgatct tggcgccttc ttccgcggcg cggcccttgg
     1501 cttcctcgac gatcgcctgg gcgcggcgct cggcgtcggc cagcagggcg gcggtttcgc
     1561 tgcgagactt ggccagttcc tcttccacgc gacggtccgc ggcggcgagg tcggccttgg
     1621 ccttgtcggc ggcagccagg ccttcggcga tcttcttcgc gcgttcgtcg agcgcgacgg
     1681 cgatcggcgg ccacacgaac ttcatcgtga acccgaccag gatcaggaac acgatgatct
     1741 gaatgatgag ggtaccggtg atgctcactt tgtcatcctt tgctcccgcg cgtcaggaaa
     1801 ccggcgggaa gggttggacc gaaaggatgg aaattacttc ggcaggttcg cgatctggcc
     1861 caggaacggg ttggcggtgg cgaaccacag ggcgataccg gtgccgatga tgaaggcggc
     1921 gtcgatcagg ccggccagca ggaacatctt ggtctgcagt tcgttcatca gctcgggctg
     1981 gcgcgcggcc gattccaggt acttgctgcc catgacgccg atgccgatgc aggcgccgat
     2041 ggcacccaga ccgatgatca ggccggcggc cagggcgaca aagctaatga cttccatgat
     2101 tgctcctaag gactttggtg gttgacgaga aaagggaaag ggaaaaaccg gtggcggcgc
     2161 ggcgcgccgc ctcagtgcgc gtcgtgggcc tggccgacgt acacgagcgt cagcatcatg
     2221 aacacgaagg cctgcagcac gatgatcagg atgtggaaga tcgcccaggc cgagcccgcg
     2281 atgatgtggc cgatggccag gccgatgccc gtcccggtaa aggagaaggc gccgcccatc
     2341 atggcgatca gcaggaagat cagctcgccg gcgtacatgt tgccgaacag tcgcatgccg
     2401 tgcgagaccg tcttggcgac gaactcgatc atctgcatgg cgaagttgaa gggccacagc
     2461 agcggatgcg ggccgaaggg ggcggtgaac agctcgtgca cccagccgcc cacgcccttg
     2521 atcttgacgt tgtagaacag gcagatcagc agcacggaga tcgacaggcc catggagatg
     2581 gacaggtcgg ccgtgggcac cacgcgcatg taggcgtgct cggggtcgtg gccggtggcg
     2641 ccgtagatgc ccttccagat ggcggggatc aggtcgaccg gcagcaggtc catcgtgttc
     2701 atcaggatga tccagatgaa gatggtcagc gccagcgggg agacgaactt gcggctggtg
     2761 gcgttgtgca cgatgccctt ggcctgggcc tcgaccatct ccaccaggat ctccaccgcc
     2821 gcctggaagc gcccgggcac gccggcggtc gcgcggcgcg
//
LOCUS       DASVLL010000503         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_518886,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000503 DASVLL010000000
VERSION     DASVLL010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518886"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VF402_08405"
     CDS             complement(<1..584)
                     /locus_tag="VF402_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7800358.1"
                     /translation="MTLYIGIGGWTYEPWRGVFYPDGLAQKRELEYAASKLSGIEING
                     TYYGSQKPESFRKWRDETPDGFKFAVKGTRYATNRRNLAESRESVERFLNSGVTELGD
                     KLGPINWQFMATKKFDAEDFENFFRLLPDRLNGVALRHAVEVRHDSFRDPAFIALAKR
                     YNIAVVTAADSEFPQIADQTADYAYLRLMGTTEG"
     gene            complement(618..>1567)
                     /locus_tag="VF402_08410"
     CDS             complement(618..>1567)
                     /locus_tag="VF402_08410"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX7800359.1"
                     /translation="GAHAASFDCVKAATAMEKTICASPDLSRADDRLAAAFGAALGGV
                     SADGRTILRANERQFLQYAADLCHPGGAADGADCLTDVFSHRAEQLDHAVVHIGGRTF
                     MPVSAFHIVPAEDGGAPCSHILTRQQIDAPATPADRDWNLWAKAAVEDAYRNSDAVSN
                     GAPVAGVDALVRMDIAAASPDLIDVSLGTAVIDMTAHNSETSAIWLTRVRRELKADDL
                     FDAGKPWATALAPLAQRRLESYDAEPPEQGFHKIRRIDEDGNWHLTPQGLRIDYPDGV
                     LADGPASAATTLLSWAELRPWLKSGLPFDPARIQVAPDR"
BASE COUNT          245 a          524 c          508 g          290 t
ORIGIN      
        1 tggccttccg tcgtgcccat cagccgcaga taggcgtagt ccgccgtctg gtcggcgatc
       61 tgcggaaact ccgaatccgc cgccgtgacg accgcgatat tgtagcgttt cgccagcgcg
      121 atgaaggccg gatcgcggaa actgtcgtgc cggacctcga ccgcatggcg caaggccacg
      181 ccgttcagcc tgtccggcag cagcctgaag aaattttcga aatcctcggc gtcgaacttt
      241 ttggtcgcca tgaactgcca gttgatcggc cccagcttgt cgcccagctc ggtcacgccg
      301 ctgttcagga agcgctcgac cgactcccgc gattcggcca gattgcgccg gttggtggcg
      361 tagcgcgtgc ccttgaccgc gaatttgaag ccgtccggcg tctcgtcgcg ccatttgcgg
      421 aacgattccg gtttttgcga accgtaatag gtgccgttga tctcgatgcc gctcagcttc
      481 gaggccgcgt attccagctc gcgtttctgc gccagaccgt cgggatagaa gacgccgcgc
      541 cacggctcat aggtccagcc gccgattccg atatagaggg tcatgatgtc ttccgtcctt
      601 gtcgtgagcg tttgcgatta ccggtcgggc gcgacctgaa tcctggcggg atcgaacggc
      661 agcccggact tcagccacgg cctgagttcg gcccaggaca aaagggtcgt agcggcggag
      721 gccgggccat cggccagcac gccgtcagga taatcgatgc gcagcccctg cggcgtcaga
      781 tgccagttgc cgtcctcgtc gatgcgccgg attttgtgga aaccttgctc cggcggctcg
      841 gcgtcatagc tttcaagacg ccgctgcgcg agcggggcca gagcggtcgc ccagggcttt
      901 ccggcgtcga acaggtcgtc cgccttaagt tcccgccgga cgcgcgtcag ccatatggcg
      961 gaggtttcgc tgttgtgcgc agtcatgtcg atcaccgcgg tcccaagact cacgtcgatc
     1021 agatcgggcg aggccgccgc gatatccatg cggaccagcg catcgacgcc cgccacgggc
     1081 gcgccgttgc tgacggcgtc ggaattgcga taggcgtcct cgaccgccgc cttcgcccac
     1141 aggttccagt cgcggtcggc gggcgtcgcc ggcgcgtcga tctgctggcg cgtcagtata
     1201 tggctgcacg gcgcgccgcc gtcttcggcg gggacgatat gaaacgcgct gaccggcatg
     1261 aaggtgcgtc cgccgatgtg tacgaccgca tggtcgagct gttcggcgcg atggctgaaa
     1321 acgtccgtca gacagtccgc gccgtccgcc gcgccgcccg gatgacacag atcggcggcg
     1381 tattgcagga actggcgctc attggcgcgc aggatcgtcc gcccatcggc ggaaacgccg
     1441 cccagcgccg ctccgaacgc cgccgccagc ctgtcgtccg ccctggacag gtccggcgac
     1501 gcgcagatcg tcttttccat cgccgtcgcg gccttcacgc aatcgaagct ggccgcgtgc
     1561 gcgccgc
//
LOCUS       DASVLL010000504         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_303700,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000504 DASVLL010000000
VERSION     DASVLL010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303700"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VF402_08415"
     CDS             complement(<1..466)
                     /locus_tag="VF402_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7800360.1"
                     /translation="MNIAVKPARRLALSLSRDHSHGPLGYLEMGRPAGPPVVFLHGFG
                     ADLLTWSLCLGPLARDYRLVALDLPGHGRTHPDVGDGTLAFMTGWLDEALDGLGLASA
                     HLVGHSMGARIALGFVLSHPARVRSLSLIAPAGLGGDFPHDALSAFLDANSPQ"
     gene            complement(463..1233)
                     /locus_tag="VF402_08420"
     CDS             complement(463..1233)
                     /locus_tag="VF402_08420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7800361.1"
                     /translation="MTFNPQFNPMNLEGRTILVTGATSGIGRATAVYLSRLGARVIAS
                     GRHQERLDAVLAELEGNGHLGRIFDLADLDAIAPWLKALCAEVGPLNGIAHCAGVQAT
                     RPIQAVKPDFVMDVLTQNLGAALMLAQGFRLKTCHGDPASLVFVSSSAALKTAPGNVV
                     YAASKGGIVSAVRGLGVELVRDGVRVNAVAPAMVDTPMSDQFRAILSEENFQRVVDMH
                     PLGLGRPDDVAAAIAFLLADTSRWITGSILTVDGGFLA"
     gene            complement(1230..>1688)
                     /locus_tag="VF402_08425"
     CDS             complement(1230..>1688)
                     /locus_tag="VF402_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HEX7800362.1"
                     /translation="DLDISHDLEHSRYTGKLKERSLTWWRKVGKAIGLQPSTLYPEYG
                     NFLIFPNIVVTFSYGAFLTFQWFEPTGPETLRIHTTSWLAKNNGRAARGMVIDSLKAF
                     SEQVRNEDRDICAIAQKGTRGMDGDRRMVLGALEGRIAHFQKAYAARMGL"
BASE COUNT          311 a          576 c          553 g          248 t
ORIGIN      
        1 cctgcggcga gttggcgtcg agaaaggccg acagcgcatc gtgaggaaag tcgccgccga
       61 ggcccgcggg cgcgatcagg ctcaggctcc tgacgcgcgc cggatgcgac aggacgaaac
      121 cgagcgcgat cctggcgccc atcgaatgcc cgaccagatg cgcggacgca aggcccagcc
      181 catcgagcgc ctcatccagc cagccggtca tgaaggccag cgtcccgtcg ccgacatcgg
      241 gatgcgtgcg gccatgtccg ggcaggtcga gcgcgaccag ccggtagtcg cgcgccagcg
      301 gccccaggca cagcgaccag gtcagcaggt ccgcgccgaa gccgtgcagg aacaccaccg
      361 gcggtcccgc cggacgcccc atctccagat agccgagcgg cccgtggctg tgatcgcgcg
      421 acagcgacag ggcgaggcgg cgcgcaggtt tcacggctat attcacgcca gaaagcctcc
      481 atcgaccgtc aggatcgaac cggtgatcca gcgcgacgtg tcggccagca ggaacgcgat
      541 cgccgccgcc acatcgtcgg gacggcccag ccccagcgga tgcatatcga cgacgcgctg
      601 gaaattctcc tccgacagga tggcgcggaa ctggtccgac atcggcgtat cgaccatggc
      661 gggcgcgacc gcgttgacgc gcaccccgtc gcgcaccagc tcgacgccca gccccctgac
      721 cgccgagacg ataccgcctt tcgaggccgc atagacgaca ttgccgggcg cggttttcag
      781 cgccgccgag gacgagacaa agaccaggga cgccggatcg ccgtgacacg tcttgagccg
      841 gaagccctgc gccagcatca gggcggcgcc gagattctgc gtcagcacgt ccatgacgaa
      901 gtcgggcttc accgcctgaa tcgggcgcgt cgcctgcacc ccggcgcaat gggcgatccc
      961 gttcagcggc ccgacctcgg cgcacagggc cttgagccag ggcgcgatgg cgtcgagatc
     1021 ggcgaggtcg aagatgcggc ccagatgacc gtttccctca agctcggcca gcacggcgtc
     1081 gagacgttcc tgatgacgcc ccgacgcgat cacccgcgcg ccgagccgcg acagatagac
     1141 cgccgtcgcg cgcccgatgc cggaggtcgc gccggtgacg aggatcgtcc gcccttcgag
     1201 gttcatgggg ttaaattggg ggttgaaggt cacaggccca tcctcgccgc ataggctttc
     1261 tggaaatgcg cgatgcggcc ttccagcgcg cccagcacca tgcgccggtc gccgtccatg
     1321 ccgcgcgttc ccttctgcgc gatggcgcag atgtcgcggt cctcgttgcg cacctgttcg
     1381 gaaaaagcct tgagactgtc gatcaccatg ccgcgcgccg cccggccatt attcttcgcc
     1441 agccacgagg tggtgtggat gcgcagcgtt tcaggccccg tcggctcgaa ccactggaag
     1501 gtcagaaagg cgccgtagga aaaggtcacg acgatattcg gaaagatcag gaagtttccg
     1561 tattcgggat aaagcgtcga cggctgaagg ccgatggcct tgcccacctt tcgccaccat
     1621 gtcagcgagc gttctttcag cttgccggta taacgcgaat gttccaggtc gtgggaaatg
     1681 tccaggtc
//
LOCUS       DASVLL010000505         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_143148,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000505 DASVLL010000000
VERSION     DASVLL010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143148"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VF402_08430"
     CDS             complement(<1..1258)
                     /locus_tag="VF402_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX7800363.1"
                     /translation="MESVNGYIRRWWKEAVVYQVYPRSFYDSNGDGVGDLRGIVEKLD
                     HIKGLGVDVVWLSPHFDSPNADNGYDIRDYRKVGAEFGTMRDFDDLLSAIHAAGMRLI
                     VDLVVNHTSDEHRWFVESRKGPDNPYRDYYIWREGDAPPNDWTSIFGGPAWKREGDSW
                     YLHLFHDKQPDLNWDNPKVRAEVHDLMRFWLDKGVDGFRMDVIPFISKDKSFPDLPPQ
                     WRSRPQYVYTQGPHVHDYLQEMRREVLDAYDTMNVGEAFGVTLARTPDFIDERRGELD
                     MAFQFAAVEVDRDENGHWKPWSLPELKAVFARQDAAFDTFCWPAVFLSNHDNPRLVSR
                     YGDDSPAWRVKSAQVLATLLLTQKGTPFLYQGDEIGLTNLRFTSPDQFNDVWSKNGWR
                     DQVESGRMRAGDFLAEQNHLSRDHARA"
     gene            1322..1415
                     /gene="ffs"
                     /locus_tag="VF402_08435"
     ncRNA           1322..1415
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF402_08435"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1427..2149)
                     /locus_tag="VF402_08440"
     CDS             complement(1427..2149)
                     /locus_tag="VF402_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7800364.1"
                     /translation="MSSRITQLTYAIGDIHGYDDLFERLIDLIRADAGRLEQRPRIVL
                     LGDYIDRGPSSPQVLERILRLEQAAWCDLVALKGNHEAALLRFLDEPAFGETWREWGG
                     GATLGAYGVDMPYLCNAPEVWRDVRDAAAKAIDPRHIEMLRRLPPLFQADDYLFVHAG
                     VDPDRPITEQDAETFMYIRGRFLRAEKACDYVVVHGHTPEDAPSDTPWRIGVDTGVYF
                     NGVLTAVRLRDGARDFLHVRSG"
BASE COUNT          440 a          739 c          684 g          420 t
ORIGIN      
        1 gcgcgcgggc gtgatcgcgg ctcagatggt tctgttcggc gaggaaatcc ccggctctca
       61 tgcgcccgct ttcgacctga tcgcgccagc cgttcttcga ccagacatcg ttgaactgat
      121 ccggcgaggt gaagcgcaga ttggtcagcc cgatctcgtc gccctgatag aggaagggcg
      181 tccccttctg cgtcagcagc agcgtggcca gaacctgcgc ggacttcacg cgccacgccg
      241 ggctgtcgtc gccatagcgc gagacgaggc gcggattgtc gtggttggac aaaaagacgg
      301 cgggccagca aaatgtgtcg aacgccgcgt cctgccgcgc gaacaccgcc ttcaattcgg
      361 gcaggctcca cggcttccaa tggccgtttt cgtcgcggtc gacctcgacg gcggcgaact
      421 gaaacgccat gtccagctcg ccgcgccgtt cgtcgatgaa atccggcgtc cgcgccagcg
      481 tcacgccgaa cgcctcgccg acattcatcg tgtcgtaggc gtccagaacc tcgcgccgca
      541 tttcctgcaa atagtcatgc acgtgcggcc cctgcgtata gacgtattgc gggcgtgagc
      601 gccattgcgg cggcaggtcg gggaagcttt tgtccttgga aatgaacggg atcacgtcca
      661 tgcggaaacc gtcgacgccc ttgtccagcc agaagcgcat gaggtcgtga acctcggcgc
      721 gcacctttgg attgtcccag ttcaggtccg gctgcttgtc gtggaacagg tgcagatacc
      781 agctatcgcc ctcgcgcttc cacgccggac cgccgaagat cgaggtccag tcattgggcg
      841 gcgcgtcgcc ttcacgccag atgtaatagt cgcgataagg attgtccggc cccttccggc
      901 tttcgacgaa ccagcggtgt tcgtccgagg tgtgattgac caccagatcg acgatcagcc
      961 gcatccccgc cgcatggatg gcgctcagca ggtcgtcgaa atcccgcatc gtcccgaact
     1021 cggcgccgac cttgcgatag tcgcggatat cgtagccgtt gtcggcgttg ggcgaatcga
     1081 aatgcggcga cagccagacg acgtcgaccc ccagcccctt gatgtgatcg agcttttcca
     1141 cgatcccgcg cagatcgccc acgccgtcgc cattgctgtc atagaacgag cgcggataga
     1201 cctgatagac caccgcctct ttccaccagc gccttatgta gccattgacc gattccatgc
     1261 cggaaagggt gagcgcaaaa atcccctctt gcaagcgggc ctgaaaatcc gcatataggc
     1321 ccccgagacg gtcgggcgaa ggcctcgcca acctggtcag ggccggaagg cagcagccat
     1381 aacgaattca ccttcgggtc ggccgtctca cctaatcccc gaaaattcag ccgctccgca
     1441 cgtgcaggaa atcgcgcgcg ccgtcccgca gccgcaccgc cgtcaggacg ccgttgaaat
     1501 agacgccggt gtcgacgccg atgcgccacg gcgtgtcgct gggcgcgtct tccggcgtgt
     1561 gtccatgcac gaccacatag tcgcaagcct tttcggcgcg cagaaagcgt ccgcgaatat
     1621 acatgaaggt ttccgcgtcc tgttcggtga tcgggcgatc cggatcgacg cccgcatgga
     1681 cgaacaggta gtcgtccgcc tgaaaaagcg gcggcaggcg gcgcaacatt tcgatatggc
     1741 gtggatcgat ggctttcgcc gccgcgtccc gcacgtcgcg ccatacctcc ggcgcgttgc
     1801 aaagataggg catgtcgacg ccataggcgc ccagggtcgc gccgccgccc cattcgcgcc
     1861 acgtctcgcc gaacgccggt tcgtccagaa agcgcagcaa cgccgcttcg tgattgcctt
     1921 tcagcgccac gagatcgcac catgcggcct gttccagcct gagaatacgc tccagaacct
     1981 gcggcgatga cggccctctg tcgatatagt cgcccagcag aacgatgcgg ggccgctgtt
     2041 ccagccgccc ggcgtcggcc cggataaggt cgatcagacg ctcgaacaga tcgtcataac
     2101 catgaatgtc gccgatggcg taggtcagtt gcgtgatgcg agaggacatg acggcataat
     2161 gcggcgaaaa tgaacgtggc ggaatggcga ttctctgtta gtcattcggc aacatagctg
     2221 acgcctgtgc aaaaagcccc tatataggtt gcatgtccga accctcagac cttgacgatc
     2281 cgt
//
LOCUS       DASVLL010000506         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_519058,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000506 DASVLL010000000
VERSION     DASVLL010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519058"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VF402_08445"
     CDS             complement(<1..614)
                     /locus_tag="VF402_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX7800365.1"
                     /translation="MSKNLLSVATLSVATLGAPRIGPRRELKFALESYWSGRSDRAAL
                     LQAARDLRAANWARQKARGVDIIPSNDFSLYDHVLDTSLMVGAIPAIYGDKADLDAYF
                     AMARGRQNGEAETCAHGHVHHGGQGGCPAQEMTKWFDTNYHYMTPEFTKDQSFALRSL
                     KPVEEFLEARALGHHTRPVLVGPVTFLKLGKGTDGVAPLSLIGRL"
     regulatory      complement(722..926)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1150..>2112
                     /locus_tag="VF402_08450"
     CDS             1150..>2112
                     /locus_tag="VF402_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800366.1"
                     /translation="MDARVFLERKGVWAFGLGCAGVTIGVLLHLPMFLMGSGMGFHLA
                     GMPMDPWMIFGMFLIVAGIGVAAYGLLPRHVAHHIETAQRISVHAPEDAPLGRAHWVL
                     MAVLVVALVIDVMKPAALGFVMPGMVAEYGVSNAHAALVPFFALVGTVCGSILWGIIA
                     DIYGRKASILLSAVVFVGTSICGAMPSLNWNIAMCFLMGAGAGGMLPVAYALLAETMP
                     SKHRGWALVLVGGLGAVGGYFAASGFSALLVPDFSWRILWLLNLPTGLILICMGGLIP
                     ESAKFLLTQGRAAEAHRIMARFGTISEKLAEGQEDASDVFAHHAH"
BASE COUNT          332 a          689 c          684 g          407 t
ORIGIN      
        1 agccgcccga tcagcgacag cggcgcgacg ccgtccgttc ccttgcccag cttcaggaag
       61 gtcacaggcc cgaccagaac cgggcgcgta tgatggccca gcgccctcgc ttcgaggaat
      121 tcctcgaccg gcttcagcga acgcagcgcg aagctctggt ccttcgtgaa ttccggcgtc
      181 atgtagtgat aattggtgtc gaaccacttg gtcatttcct gcgccgggca gccgccctgc
      241 ccgccatgat gcacatgacc gtgcgcgcag gtttcggcct cgccgttctg acggcccctg
      301 gccatggcga aatacgcatc gagatcggcc ttgtcgccat agatggccgg gatggcgccc
      361 accatcagcg acgtgtccag cacatgatcg tacagcgaaa aatcgttgga cgggatgatg
      421 tccaccccgc gcgccttctg acgcgcccag ttggcggcgc gcagatcgcg cgcggcctgc
      481 aacagcgcgg cccggtcgga ccggcccgac cagtaggatt ccagcgcgaa tttcagttcg
      541 cgcctcgggc cgatgcgcgg cgcgcccaat gtggcgacag atagcgtagc gacggataat
      601 aggttttttg acacggcttt ccccgtaagg ctgatcggac cggggcggcg ggcgcgcgcc
      661 gacccccgcc gccctgtcga aggccgcgcg ttccgacgcc gtcccgcggc cctccgcccg
      721 gaatggatat gcggtcgctg gcaggtctcc tgactcgcgg gtcgtcgcct cgatccgcct
      781 tctcgggagg cggtcatgtc ggaccggtct cccaatggcg tgatggatgt cggctcgccg
      841 ctcacagttg cgggggcagc gccggactcg caccggcttc cctcttggct ccgcgccccc
      901 ggcgaaccgg atgcgcaaag aaccaacgac gccgccaccc taggcggatc ggcgccgccc
      961 gtcaatatga tataaagata tatttatatc gttatatatt tatgtcgcgc cccggaaagg
     1021 ccgaaagcgt ttccgggaat gttgccaacg tagcaacaat gaaaaatata caatacattg
     1081 aattttttag ttttaattcc gaaaaaactc tgccttatgt tcgcaaaaag cattggcgga
     1141 gggcgaaata tggacgcgcg ggtttttctc gaacgcaagg gcgtgtgggc gttcgggctg
     1201 ggctgcgcgg gcgtcaccat cggcgtgctg ctgcatctgc cgatgttcct gatgggcagc
     1261 ggcatgggtt ttcatctggc cggcatgccg atggacccgt ggatgatctt cggcatgttc
     1321 ctgatcgtgg cgggcatcgg cgtcgccgcc tacggcctgt tgccgcgcca cgtcgcgcac
     1381 catatcgaga cggcgcagcg catatcggtc catgcgcccg aggacgcgcc gctcggccgc
     1441 gcccactggg tgctgatggc cgtgctggtc gtcgcgctcg tcatcgacgt gatgaagccc
     1501 gccgcgctcg gcttcgtcat gcccggcatg gtcgcggagt acggcgtaag caacgcccac
     1561 gccgctctgg tgccgttctt cgcgctggtc ggcacggtct gcggctcgat cctgtggggg
     1621 atcatcgccg acatctacgg gcgcaaggcg tcgatccttc tgtccgccgt ggtgttcgtc
     1681 ggcacctcga tctgcggcgc catgccgtcg ctgaactgga acatcgccat gtgctttttg
     1741 atgggcgcgg gcgcgggcgg catgttgccg gtcgcctatg cgctgctggc cgagaccatg
     1801 ccgtccaagc atcgcggctg ggcgctggtg ctggtcggcg gcctcggcgc ggtcggcggc
     1861 tatttcgccg cctccggctt ttcggccctg ctggtgcccg acttctcgtg gcgcatcctg
     1921 tggctgctga acctgccgac cgggctgatc ctgatctgca tgggcgggct gatcccggaa
     1981 tcggccaagt tcctgctgac gcagggccgc gccgccgagg cgcaccgcat catggcgcgc
     2041 ttcggcacga tcagcgagaa gctggccgaa ggtcaggagg acgccagcga cgtattcgcg
     2101 caccacgcgc ac
//
LOCUS       DASVLL010000507         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_194679,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000507 DASVLL010000000
VERSION     DASVLL010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194679"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VF402_08455"
     CDS             <1..915
                     /locus_tag="VF402_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800367.1"
                     /translation="RFAAAKVMARPLLGNLSPAVTSISVPNTGNTTGGTLTIGNPKLS
                     PFRADNFDADVEWYFKKNALLSFAVFTKKINSYPQTVIYSAPLSTFLSADNIAALRQQ
                     FSNANQLNYIDSDYTFDARQYRDAPGGTLSGWEFNYQEDLDFLPGVLKNLGVQFNATH
                     INSKLTYILDPGSATTAATYGSGPWLGASPDAQNLTVYYEVPKFSVRVSVARRDGYYT
                     NYPIAAGSCAPGACDSPLVNDFAGSKGTTNVDAVASWNFNAHLSLRLEGLNLSNQTTN
                     RYAYVASPVVTQYASSGRQITLGVRFKY"
     gene            1069..>2399
                     /locus_tag="VF402_08460"
     CDS             1069..>2399
                     /locus_tag="VF402_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEX7800368.1"
                     /translation="MTFTRRHAAISLGASLGAFLAAPLAARAASGETPGPLSVWHRQP
                     AAVWTEASPIGNGRLGAMVYGGVGREELQLNEDTLWAGGPYDPTHKGAVKNLAEVRRL
                     IFAGRYKDAEALIEQGMMAQPERQMSYQTIGSLFLTAGPSSMASDYVRELDLDRALSR
                     VGFTQDGARITRETFASPVDQVIVMRVRADRPGGVRLRLDYGTPQAARAAVDGADLIL
                     SGVNTSQQGVPAALRFEARVRVRATGGRLVPDGDGLILAGADEALILIAAATSFKSWS
                     DTSGDPAALNTAVLANAEGKSFDDLLAAHVAAHRRLFRRVALDLGQGEAASLPTDARV
                     NRADLASDPGLAALYYQFGRYLMIACSRPGTQAAGLQGLWNDRLNAPWGGKYTININT
                     EMNYWPAERGALPECVGPLVDLVCDIARRGEETAREHYGARGWVCHHNTDI"
BASE COUNT          396 a          856 c          767 g          380 t
ORIGIN      
        1 cgcttcgccg ccgccaaggt gatggcgcgg ccgctgctgg gcaacctgtc gccggcggtc
       61 acctcgatca gcgtgccgaa caccggcaac accacgggcg gcacgctgac catcggcaac
      121 cccaagctca gcccgttccg cgccgacaat ttcgacgccg acgtggaatg gtacttcaag
      181 aagaacgcgc ttctgtcgtt cgcggtcttc accaagaaga tcaactccta cccgcagacg
      241 gtgatctatt cggcgccgct atcgaccttc ctgagcgcgg acaatatcgc ggcgctgcgc
      301 cagcagttct ccaacgccaa ccagttgaac tatatcgaca gcgactacac cttcgacgcg
      361 cgccagtatc gcgacgcgcc gggcggcacg ctgtcgggct gggagttcaa ctatcaggag
      421 gatctcgact tcctgccggg cgtgctgaag aatctcggcg tgcagttcaa cgccacgcac
      481 atcaattcca agctgaccta catcctcgat ccgggcagcg cgacgacggc ggcgacctac
      541 ggttcggggc cgtggctggg cgcctcgccc gacgcgcaga acctgaccgt ctattacgaa
      601 gtgcctaaat tcagcgtgcg cgtgtcggtc gccaggcgcg acggctatta caccaactat
      661 ccgatcgccg ccggatcgtg cgcgccgggc gcgtgcgatt cgccattggt caacgacttc
      721 gccggctcca agggcacgac caatgtcgac gccgtcgcca gctggaattt caacgcccat
      781 ctgagcctgc gcctcgaagg cctgaacctg agcaaccaga cgaccaaccg ctacgcctat
      841 gtcgccagcc cggtcgtcac ccagtacgct tccagcggcc gccagatcac cctgggcgtg
      901 cgcttcaagt attaagcttg tcccgccggc gctcttcccc cgccggcggg caagacccca
      961 gctccccgtt ttgcgccgaa gcctcagcgc gcgtcagctg gattcctggc gccggtttgc
     1021 aagaatcggc gcccttttta ttccgaacgc ccatgcggga gccgttccat gaccttcacc
     1081 cgccgtcacg ccgccatctc tctgggagcc tcgttgggcg cctttctggc cgcgccgctg
     1141 gccgcgcgcg ccgcgtccgg agagacgccc ggcccgctga gcgtgtggca caggcaaccc
     1201 gcggcggtct ggaccgaagc ctcgcccatc ggcaacggac ggctgggcgc gatggtctat
     1261 ggcggcgtcg ggcgcgagga gttgcaactc aacgaggaca cgctctgggc aggcggacct
     1321 tacgatccga cgcacaaggg cgcggtgaaa aacctcgccg aggtccgccg cctgatcttc
     1381 gccgggcgct ataaggacgc cgaggccctg atcgagcagg gcatgatggc ccagccggag
     1441 cgccagatgt cgtatcagac gatcggcagc ctgtttctca ccgccgggcc gtcgtcgatg
     1501 gcctcggact atgtgcgcga actcgatctc gaccgcgcgc tgagccgcgt cggcttcacg
     1561 caggacggcg cgcgcatcac gcgcgaaacc ttcgcctcgc cggtcgatca ggtcatcgtc
     1621 atgcgcgtcc gcgccgatcg gcccggcggc gtgcggctgc gcctcgacta cggcacgccg
     1681 caggcggcgc gggccgccgt cgacggcgcc gacctgatcc tgagcggcgt caacaccagc
     1741 cagcagggcg tgcccgccgc cctgcggttc gaggcgcgcg tccgcgtgcg cgcgacaggc
     1801 ggacggctgg tccccgacgg cgacgggctg atccttgcgg gcgccgacga ggcgctgatc
     1861 ctgatcgccg ccgcgaccag cttcaaatcg tggagcgaca cgtccggcga tccggcggcg
     1921 ctcaacacgg ccgtgctggc caacgccgag ggtaaatcgt tcgatgattt gctggccgcg
     1981 cacgtcgccg cgcaccggcg cctgttccgg cgcgttgcgc tcgatctggg ccaaggcgag
     2041 gccgcgtccc tgccgaccga tgcgcgcgtc aaccgcgccg atctggcctc cgatccgggg
     2101 ctggccgcgc tctattacca gttcggacgc tatctgatga tcgcctgttc gcggcccgga
     2161 acgcaggcgg cgggcctgca agggctgtgg aacgacaggc tgaacgcgcc atggggcggc
     2221 aaatacacca tcaatatcaa caccgagatg aactactggc ccgccgagcg cggcgccttg
     2281 cccgaatgcg tcgggccgct ggtcgatctg gtgtgcgaca tcgcccggcg cggcgaggag
     2341 acggcgcgcg agcattacgg cgcgcgcggc tgggtctgcc accacaatac cgacatctg
//
LOCUS       DASVLL010000508         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_87645, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000508 DASVLL010000000
VERSION     DASVLL010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87645"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2429
                     /locus_tag="VF402_08465"
     CDS             <1..>2429
                     /locus_tag="VF402_08465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7800369.1"
                     /translation="VRPAQFCAPSAAGLALACGFDEPETPQDASRALFDVIDSLVRTV
                     TTAPLGFRLAAASGVKLMEKQGWAWAPLLDAALARTDMPSDYAPPPALDVWTLLEEWE
                     DQPPEGQARAVALSEAEVTEALNRDLQRANLSERRPEQLDFALKARDIFAPKWMTDQP
                     NLLLAEAGTGTGKTLGYLAPAHLWAKKAQGQVWVSTYTKALQKQIYNDTRALFDTAEE
                     RAREVVVRKGRENYLCLLNFQERLAAAHSAPEVIMAVLMARWALYTRDGDIMSGDFPT
                     WLPSLLPPTAPAHVFQTMTPQSLVDRRGECTYAACAHYRVCFIEKSVRKAKSASLVVA
                     NHALVMAQAAYDLLDRQRKEGEDEAAPALSRLIFDEGHHVFEAADGAFATRLSGLEAY
                     ELRRWIRGNEGRSRRGRGLEARLGDLIAQDETAKSALRDLLRAAVVLPSDGFSQRMAV
                     RTQSGGGLEPIGPIEALLTEVHNQIEVRKGEGTLRARFSPDNDPGGECEAHPCLDGVA
                     QAAKIAARALSGIEAPLLALVRALEDRLSAAGDELEAPDRIRIEGALRGLERRARHLL
                     PAWRHLLQSLAEEDFESGEPDPDKAQAPAQTIDWFSTELHRGRVADVALNRHYVDPSL
                     PLAEFVLKPAHGVLVASATLSDGYVKDDAGEGDAPDAFSLARQRTGANHLVMGAKTAR
                     IATPFDYAAQARLFVVTDVSREDARSVGAAMLALFKAARGGGLGLFTAIRRLRAVYDQ
                     IATPLSEAGLPLYAQHVDPLDVSDLISVFRAERHSCLLGTDAVRDGVDVPGQALRLIA
                     FDRVPWPRPDC"
BASE COUNT          356 a          829 c          881 g          363 t
ORIGIN      
        1 cgtgcgtccg gcgcagttct gcgcgccgtc ggcggcgggg ctggccctgg cgtgcggctt
       61 cgacgagccg gagacgccgc aggacgcgtc gcgcgcgctg ttcgacgtga tcgacagcct
      121 cgtccgtaca gtgacgaccg cgccgctcgg cttccggctg gcggcggcca gcggcgtgaa
      181 actgatggaa aagcagggct gggcgtgggc gccgctgctg gacgccgcgc tggcccggac
      241 cgacatgccg tccgattacg cgccgccgcc cgcgctcgac gtgtggaccc ttctggagga
      301 gtgggaggac cagccgcccg aaggtcaggc gcgcgccgtg gcgctgagcg aggccgaggt
      361 gaccgaggcg ctgaaccgcg acctgcaacg cgccaacctg tccgaacgcc gcccggagca
      421 gctcgatttc gcgctgaagg cgcgcgacat cttcgcgccg aaatggatga cggaccagcc
      481 gaacctgctg ctggccgagg cgggcaccgg caccggcaag acgctcggct atctcgcgcc
      541 cgcgcacctg tgggccaaga aggcgcaggg gcaggtctgg gtctcgacct ataccaaggc
      601 gctgcaaaag cagatttata acgacacgcg cgccctgttc gacacggcgg aggaacgcgc
      661 gcgcgaggtc gtggtgcgca aggggcgcga gaactatctg tgcctgctca attttcagga
      721 gcgcctcgcc gccgcccact ccgcgcccga agtcatcatg gccgtgctga tggcgcgctg
      781 ggcgctctat acgcgcgacg gcgacatcat gagcggcgat ttcccgacct ggctgccgtc
      841 gctgctgccg ccgaccgcgc ccgcgcatgt gttccagacg atgacgccgc aaagcctggt
      901 cgaccggcgc ggcgaatgta cctacgcggc ctgcgcgcac taccgggtct gcttcatcga
      961 gaaatcggtg cgcaaggcga aatccgcctc gctggtggtc gccaatcatg cgctggtgat
     1021 ggcgcaggcg gcttacgacc tgctcgaccg ccagcgcaaa gaaggcgagg atgaagccgc
     1081 gcccgccctg tcgcggctga tcttcgacga gggccaccat gtgttcgagg cggcggacgg
     1141 cgccttcgcg acccgtctat cggggctgga ggcctacgaa ctgcggcgct ggatacgcgg
     1201 caacgaaggc cgttcgcgcc ggggccgggg gctggaggcg cggctgggcg acctgatcgc
     1261 gcaggacgag acggccaaat cggcgctgcg cgacctgctg cgcgccgccg tcgtcctgcc
     1321 ctcggacggc ttttcgcagc gcatggcggt gcgcacgcaa agcggcggcg ggctggagcc
     1381 gatcggcccc atcgaggcgc ttttgaccga agtccacaac cagatcgagg tgcgcaaggg
     1441 cgaggggact ttgcgcgcgc gtttttcgcc ggataacgat ccgggcggcg aatgcgaggc
     1501 gcatccgtgt ctcgacggcg tggcgcaggc ggcgaagatc gcggcgcgcg ccctgtcggg
     1561 catcgaggcg ccgctgctgg cgctggtgcg cgcgctggag gaccggctga gcgcggcggg
     1621 cgacgaactg gaggcgcccg accgcatccg catcgaaggc gctctgcgcg ggctggagcg
     1681 gcgcgcgcgc cacctgctgc ccgcctggcg gcatctgctg caaagcctgg ccgaggagga
     1741 ttttgaaagc ggcgaaccgg acccggataa ggcccaagcc ccggcccaga cgatcgactg
     1801 gttctcgacc gaactgcatc gcgggcgcgt ggccgatgtg gcgctgaacc ggcactatgt
     1861 cgatccgtcc ctgccgctgg ccgaattcgt gctgaagccc gcgcacggcg tgctggtcgc
     1921 ctcggccacc ctgtcggacg gctatgtcaa ggatgatgct ggagaaggcg atgcgcccga
     1981 cgccttttcg ctggcgcggc agcgcaccgg cgccaaccac ctcgtcatgg gcgccaagac
     2041 cgcgcgcatc gccacgccgt tcgactatgc ggcgcaggcg cgtttgttcg tcgtcaccga
     2101 cgtgtcgcgt gaggatgcgc gctctgtcgg cgccgccatg ctggccctgt tcaaggcggc
     2161 gcgcggcggg gggctgggcc tgttcaccgc catccgccgc ctgcgcgccg tctatgacca
     2221 gatcgcgacg ccgctcagcg aggcggggct gccgctgtac gcgcagcatg tcgatccgct
     2281 cgatgtctcc gacctgatca gcgtgttccg cgccgagcgg cactcctgcc ttttgggcac
     2341 cgacgcggtg cgcgacggcg tcgatgtgcc ggggcaggct ttgcgcctga tcgccttcga
     2401 ccgcgtgccg tggccgcgcc cggactgtt
//
LOCUS       DASVLL010000509         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_143483,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000509 DASVLL010000000
VERSION     DASVLL010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143483"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VF402_08470"
     CDS             <1..507
                     /locus_tag="VF402_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7800370.1"
                     /translation="LQKGVVGLPLVHPVEGVIARTRTLLDAFRAAGRTVVLVNVDGVA
                     PGRTEEPRRVLDLPDGWTDLIPELDAQPGDLRVTKRSWGAFPTTDIEAQLRARGVTQV
                     VVTGVATATGVEATARQAFEAGFNVTLAVDAMTDMRADAHAYSLAKVFPRLGETGTAD
                     EIVALLRA"
     gene            504..>1645
                     /locus_tag="VF402_08475"
     CDS             504..>1645
                     /locus_tag="VF402_08475"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800371.1"
                     /translation="MSEAAPAGGMFRSLRLRNYRVWAAGALVSNIGAWVQRTAQDWLV
                     LTELTHHDASAVGLVMALQYAPQFLLLPWTGMAADRLNQRRLLMATQAVMGVLALTLG
                     ALVVLGAAKLWHVCVFAFLLGCAAAFDAPVRQTFVSELVGNDDLPNAVALNSTSFNAA
                     RMIGPAAAGLAVAAIGSGWAFVFNGLSFFAVLASLFFLRRDELRPNPRAARARGGFIE
                     SLRYVSGRPDLKAILLVLFLIGTFGLNFPIFISTMTVSAFHGDARAYGLLSAAMAVGT
                     LGGALMNARRTRPVFGSLLNGAGLFGLGCALAALAPSPLLFAAALVMTGAAALTVTNT
                     SNSLIQISTDPVMRGRMMALRVGVALGGTPIGAPLVGWVANACGPRA"
BASE COUNT          194 a          601 c          576 g          274 t
ORIGIN      
        1 ctgcaaaagg gcgtggtcgg cctgcccctc gtccatccgg tcgaaggcgt catcgccagg
       61 acgcgcacgc tgctcgacgc gttccgcgcg gcgggccgga cggtcgtgct ggtcaatgtc
      121 gacggcgtcg cgccgggccg caccgaggag ccgcgccgcg tcctcgacct gcccgacggc
      181 tggaccgacc tgatccccga actggacgcg caacccggcg atcttcgcgt caccaagcgt
      241 agctggggcg ccttcccgac caccgacatc gaggcgcagt tgcgcgcgcg cggcgtcacg
      301 caggtggtcg tcaccggcgt cgccaccgcc accggcgtgg aagcgaccgc gcgtcaggct
      361 tttgaagcgg gcttcaacgt gaccctcgcc gtcgacgcca tgaccgacat gcgcgccgac
      421 gcccacgcct acagcctcgc caaggtcttt ccgcgcctcg gcgagaccgg aacggcggac
      481 gagatcgtcg cgcttctgcg cgcatgagcg aggccgcgcc cgcaggcggc atgttccgct
      541 ccttgcgcct gcgcaattac cgcgtctggg cggcgggcgc gctggtgtcg aacatcggcg
      601 cctgggtgca gcgcacggcg caggactggt tggtcctgac cgagctgacg catcacgacg
      661 cctcggcggt cggcctcgtc atggcgctgc aatacgcgcc gcaattcctt ctgctgccgt
      721 ggaccggcat ggctgccgac cgcctgaacc agcgccgcct tttaatggcg acgcaggcgg
      781 tcatgggcgt gctggccctg acgctgggcg cgctggtcgt gctgggcgcg gcgaagctgt
      841 ggcatgtgtg cgtgttcgcc ttcctgctcg gctgcgcggc ggcgttcgac gcgccggtgc
      901 gccagacctt cgtcagcgaa ctggtcggca atgacgacct gcccaacgcc gtggcgctca
      961 actcgacctc gttcaacgcc gcgcgcatga tcggcccggc ggcggcgggg ctggccgtgg
     1021 ccgccatcgg ctccggctgg gcgttcgtgt tcaacggcct gtccttcttc gccgtgctgg
     1081 cctcgctgtt tttcctgcgc cgcgacgagc tgcggcccaa tccgcgcgcg gcgcgggcgc
     1141 gcggcggctt tatcgagagc ctgcgttatg tgagcgggcg gccggacctc aaggcgatcc
     1201 tgctggtcct gttcctgatc ggcacgttcg ggctgaactt cccgatcttc atctcgacca
     1261 tgacggtgag cgcctttcac ggcgacgcgc gcgcctacgg cctgttgtcg gcggcgatgg
     1321 ccgtcggcac gctgggcggc gcgctgatga acgcgcgccg gaccagaccc gttttcggct
     1381 cgctgctgaa cggcgcgggg ctgttcggcc tcggctgcgc gctggcggcg ctggcgccgt
     1441 cgccgctgtt gttcgccgcc gcgctggtca tgaccggcgc cgccgccctg accgtgacca
     1501 acacctccaa cagcctgatc cagatctcga ccgacccggt gatgcgcggc cgcatgatgg
     1561 ccttgcgcgt cggcgtcgcg ctgggcggca cgccgatcgg cgcgcccctc gtcggctggg
     1621 tcgccaacgc ctgcggcccg cgcgc
//
LOCUS       DASVLL010000510         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_519254,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000510 DASVLL010000000
VERSION     DASVLL010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519254"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VF402_08480"
     CDS             complement(<1..1322)
                     /locus_tag="VF402_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX7800372.1"
                     /translation="MRLTRRGLIGTAGASGLTAGAARAKGASGPFAGTWESLADGYRT
                     PDWFRDAKFGIWAHWGPQCIPEWGDWYGRQMYQQGNPFYEHHVKTYGHPAQFGFMQFL
                     KDWKIDQWEPDRLLSLYKKAGARYFVALAGHHDNFDMFDSRYQAWNVTKLGPKVDTMA
                     GWAKAARDHGLRFGLSNHLAHAWHWWQTAYGYDPEGPVRGLRYDAYRLSKADGAGQWW
                     DGYDPQELYTGPHGDMPAPDGIDTIKAMNAYTDAHSGQWLETVPPGDPYYVKKWLARA
                     NDMVEKYRPDLVYYDDTALPLEQAGLDATAYYYNQSLRWHGKLDVVATGKKLSDLQRR
                     AIVEDVERGFSDTLRPIPWQTDTCIGNWHYDRPLYERNGYKSAKDVIQRLCDVVSKNG
                     NLLLSIPVRGNGAIDEKEEKIVEDIAGWMAVNGEAIFATRPWDIYGEGP"
BASE COUNT          280 a          586 c          559 g          332 t
ORIGIN      
        1 gggccttcgc catagatgtc ccacggacgg gtggcgaaga tcgcctcgcc gttgaccgcc
       61 atccagcccg cgatgtcctc gacgatcttc tcctccttct cgtcgatggc gccgttgccg
      121 cgcaccggga tcgacagcag caggttgccg ttcttcgaca ccacgtcgca caggcgctgg
      181 atcacgtcct tggcgctttt atagccgttg cgctcataga gcgggcggtc gtagtgccag
      241 ttgccgatgc aggtgtcggt ctgccacggg atcgggcgca gcgtgtcgga aaagccgcgc
      301 tcgacgtcct cgacgatggc gcggcgttgc aggtcgctga gcttcttgcc ggtggcgacc
      361 acgtccagct tgccgtgcca tctgaggctc tggttgtaat aataggcggt ggcgtccagc
      421 ccggcctgct ccagcggcag ggcggtgtcg tcataataga cgaggtcggg ccggtacttt
      481 tcgaccatgt cgttggcgcg cgccagccac tttttgacgt agtagggatc gcccggcggg
      541 accgtctcca gccattgccc ggaatgtgcg tcggtatagg cgttcatcgc cttgatggtg
      601 tcgatgccgt ccggcgcagg catgtcgccg tgcgggccgg tatagagttc ctgcgggtcg
      661 tagccgtccc accactggcc cgcgccgtcc gccttgctca gccggtaggc gtcgtagcgc
      721 aggccgcgca ccggcccttc cgggtcgtag ccataggcgg tctgccacca atgccaggcg
      781 tgcgccagat ggttggacag accgaagcgc aggccgtgat cgcgcgccgc cttcgcccag
      841 cccgccatgg tgtcgacctt cggccccagc ttcgtgacgt tccatgcctg atagcgggaa
      901 tcgaacatgt cgaaattgtc gtgatgcccg gccagggcga cgaaatacct ggcgcccgcc
      961 ttcttgtaga ggctcagaag ccggtccggc tcccactggt cgatcttcca gtccttgagg
     1021 aactgcatga agccgaactg cgccggatgg ccgtaggtct tgacgtggtg ttcgtagaac
     1081 ggattgccct gctggtacat ctgccgcccg taccagtcgc cccattcggg gatgcattgc
     1141 ggcccccagt gcgcccagat gccgaacttg gcgtcgcgga accagtccgg cgtccggtag
     1201 ccgtccgcca gcgactccca cgtcccggcg aaggggccgg aagcgccctt cgcccgcgcc
     1261 gcgcccgccg tcaggccgct ggccccggcg gtgccgatca gtccccgtcg cgtcagtctc
     1321 atgcgttctt cctgtcgaaa ggcggcgcgg gcggcgtcgc cgttttttat atgcagcgtg
     1381 attgatcgta tatgaacaag cggcggagat caaaccgcca gcggtaaaat cagcgcgacc
     1441 tcaattccgc atacgcaaca aggcgccatt agtaaatttt cgtttgccgc cgcgcgcaag
     1501 gacgcctaac atgtccgaca atagaacagc cgccatgcag gcggcggacg cgcgcccgct
     1561 tcacgggctg cgacgacagg aggaaaaatc ccatgtttat caccgccatc gcggccgcgt
     1621 ccgcctttct gatccagtcg ccggacaaga ccctggccat gcaggtgtcg agggacggat
     1681 cgcagttcac cgtccggcgc aagggcgagg agatcatcgc gccctcgccc ctgggcctga
     1741 tccccgccga cgcgccg
//
LOCUS       DASVLL010000511         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_194946,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000511 DASVLL010000000
VERSION     DASVLL010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194946"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VF402_08485"
     CDS             <1..150
                     /locus_tag="VF402_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7800373.1"
                     /translation="DGEIMGLQHRTRPIHGVQFHPESIATEGGHQLLANFLDLAGVKT
                     QNLYV"
     gene            143..1165
                     /gene="trpD"
                     /locus_tag="VF402_08490"
     CDS             143..1165
                     /gene="trpD"
                     /locus_tag="VF402_08490"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7800374.1"
                     /translation="MSDAFKPLLARLADGTTLNEADAEAFFDACLRGEPTPAQVAAAV
                     TAIRIRGETVGELAACARAMRKAAIHLDHPYQVIDVCGTGGDGLHTLNISTAVGFVAA
                     GGGLKVAKHGNRAITSKSGTADVLAALGVNLEPTQAQQRAALDTAGICFMFAQAHHGA
                     MRHVSPIRQQLGFRTIFNLLGPLSNPAGAQRQVVGVPSIRFVEPIAQALGQLGAQKAW
                     VVHGSGLDELTTTGETEVAEWRDGFVRLFHITPEAVGLPRAALADLIGGAPEHNAREL
                     AELLDGKTGPYRDIVLLNAAAAFLVADKVETLREGVDLAAHVIDAGAARAALDALIRA
                     TAPIES"
     gene            1168..>1910
                     /gene="trpC"
                     /locus_tag="VF402_08495"
     CDS             1168..>1910
                     /gene="trpC"
                     /locus_tag="VF402_08495"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX7800375.1"
                     /translation="MSDILEKIAAYKREEVAARKASGADLDARLETASPPRGFMTALT
                     RDRKPGALSLIAEIKKASPSKGLIRQDFDPPGLARAYAAGGARCLSILTDAPSFMGCE
                     SYFESARAAVDLPCIRKEFLVDVWQVAESRALGADAILVIMAMIDDALAVDLIDAAHD
                     LGMDALVEVHDRAEMARALRLKSPLIGINNRNLRTFEVSLATTEDLAHQVGPEHVLVA
                     ESGVFTPADVARCAQAGAAAMLVGESLMR"
BASE COUNT          321 a          682 c          621 g          286 t
ORIGIN      
        1 gacggcgaga tcatggggct gcaacaccgc acgcgcccga tccacggcgt gcagtttcac
       61 cccgaatcga tcgccaccga gggcggccat cagttgctgg ccaacttcct cgacctggcc
      121 ggcgtgaaga cgcaaaacct ctatgtctga cgccttcaaa cctttgctgg ccagactggc
      181 tgacggaacg accctgaacg aggccgacgc cgaagccttt ttcgacgcct gcctgcgcgg
      241 cgagccgacc ccggcgcagg tcgccgccgc cgtcaccgcc atccgcatcc gcggcgagac
      301 ggtcggcgaa ctggccgcct gcgcgcgcgc catgcgcaag gccgccatcc acctcgacca
      361 tccgtatcag gtcatcgacg tgtgcggcac cggcggcgac ggcctgcaca cgctcaacat
      421 ctccaccgcc gtcggcttcg tggcggcggg cggcgggctg aaggtcgcca agcacggcaa
      481 ccgcgccatc acctcgaaat ccggcacggc ggacgtgctg gcggcgctgg gcgtcaatct
      541 ggagccgacg caggcgcagc aacgcgcggc gctcgatacg gcgggcatct gcttcatgtt
      601 cgcgcaggcg catcacggcg ccatgcgcca cgtctcgccg atccgccagc aactcggctt
      661 ccgcaccatt ttcaacctgc tgggaccgct gagcaatccg gcgggcgcgc agcgtcaggt
      721 ggtcggcgtg ccgtcgatcc gcttcgtcga gccgatcgcg caggcgctgg gccagctcgg
      781 cgcgcaaaag gcgtgggtcg tgcatggctc cggtctcgac gaactgacca cgaccggcga
      841 aaccgaggtc gccgaatggc gcgacggctt cgtgcgcctg ttccacatca cgccggaggc
      901 ggtcggcctg ccgcgcgcgg ccctggccga cctgatcggc ggcgcgccgg aacataatgc
      961 gcgcgaactg gccgaattgc tcgacggcaa gaccggacct taccgcgaca tcgtgctgct
     1021 gaacgccgcc gccgcctttc tggtcgccga caaggtcgag accctgcgcg aaggcgtcga
     1081 cctcgccgcg catgtgatcg acgccggggc ggcgcgggcg gcgctggacg ccttaattcg
     1141 ggcgaccgcg cccatagaga gctaaaaatg agcgatattc tggaaaaaat cgccgcttat
     1201 aaacgcgagg aagtcgccgc gcgcaaggcg tccggcgccg atctcgacgc gcgcctcgaa
     1261 accgcctcgc cgccgcgcgg cttcatgacg gccctgacgc gcgaccgcaa gcccggcgcc
     1321 ctgtcgctga tcgccgaaat caagaaggcg tcaccctcca agggcttgat tcgtcaggat
     1381 ttcgacccgc caggcctggc ccgcgcctat gcggcgggcg gcgcgcgctg cctgtcgatc
     1441 ctgaccgacg cgcccagctt catgggctgc gaatcctatt tcgaatcggc ccgcgccgcc
     1501 gtcgacctgc cctgcatccg caaggaattc ctggtcgatg tctggcaggt cgccgaatcg
     1561 cgcgcgctgg gcgccgacgc gattctggtc atcatggcga tgatcgacga cgcgctggcc
     1621 gtcgacctga tcgacgcggc gcacgacctg ggcatggacg cgctggtcga ggtgcacgac
     1681 cgcgccgaaa tggcgcgcgc cctgcgtctg aaatctccgc ttatcggcat aaataacaga
     1741 aatctcagaa ctttcgaggt cagcctcgcc acgaccgaag acctggccca tcaggtcgga
     1801 ccggagcatg tcctggtggc ggaaagcggc gttttcaccc ctgccgacgt cgcgcgctgc
     1861 gcgcaggcgg gcgccgccgc catgctggtc ggcgaaagcc tgatgcggca
//
LOCUS       DASVLL010000512         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_519556,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000512 DASVLL010000000
VERSION     DASVLL010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519556"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF402_08500"
     CDS             <1..220
                     /locus_tag="VF402_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEX7800376.1"
                     /translation="PGADGRPVRFRVTLDGHAPGADHGADTDANGDGVVSGDRLYQLI
                     RQTGKVGDRTFAITFLDPGVEAFSFTFG"
     gene            complement(245..2137)
                     /locus_tag="VF402_08505"
     CDS             complement(245..2137)
                     /locus_tag="VF402_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7800377.1"
                     /translation="MIRFRPRRADPPPSRRQARQEEQGLAAAMCGNMRAFLLFAIAYF
                     VVNTLFYRVAGDPGWLPALSGATLIACGLFWVWNRRAASMTRLEITSHVLGFLFIANA
                     LLDICLAYQPVKLLFLLLLLPAFSISGARLRAALPSSLAAVAAFVWAAWRFERAHFGD
                     YLWVAFAGVIIGAGLAGVIRMSMREAVRARTRADAHRDEALSLAAFDALTGLANRRAF
                     LNVAEQRLSEGGRFYLGLVDLDGFKPINDIFGHAAGDRVLVEVARRLREVCGPEAVVA
                     RLGGDEFALILPDPPGGEAWRARADRISEHLARPYALDAETAHLSASLGFALHDENQP
                     AAAPSKLLERADYALYEAKEKGRGVAVLFEPHHEALIRNAKAVEQALRGIDSEKELTL
                     AFQPQRDVRGERTLAFEALARWRNARLGQVPPDVFIRAAERSGLITGLTPVLLKKALK
                     AARRWPGDLRLAFNLSARDILSPAAVAKIAQVVRESGVDARRIEFEITETAMLTDFDR
                     ARANIAALRALGCRMALDDFGSGYSNFSYLNRVEVDTVKIDRSFVQALGRGEKAREII
                     KSMIELAQNLGMDHVIEGVETEAEMHQLRLAGARQVQGFLYGRPMDEADIAAFLRAEH
                     DARLTG"
     gene            complement(2254..>2826)
                     /locus_tag="VF402_08510"
     CDS             complement(2254..>2826)
                     /locus_tag="VF402_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800378.1"
                     /translation="LRGFAISALGSITYYVGIGYVPAFISSTGALPESQALWLSTIAA
                     VAVIAVTPVVGALSDRLGRRPVLVALCLASAVLPTALFSLMASGAAASALLGAVTLAA
                     VAGGVSAVGAVATAEQFPGEGRLSGLALGATVATALFGGFTPYAAQRLIETTHWPVAP
                     GLMIAVVAVCVLPVLLRLPETAVRAADRRG"
BASE COUNT          473 a          985 c         1002 g          366 t
ORIGIN      
        1 gccgggcgcg gacggcaggc ccgtgcgctt ccgcgtcacg ctcgacggcc acgcgccggg
       61 cgccgaccac ggcgcggata ccgacgcgaa cggcgacggc gtggtgagcg gggaccggct
      121 ctatcagttg atccggcaga cgggtaaggt cggggaccgg acgtttgcga ttacgtttct
      181 ggaccccggt gtggaggcat tttcctttac gttcgggtag ggggagaagg caggcgccac
      241 aacctcaccc cgtcaaccgc gcgtcgtgtt cggcgcgcag gaaggcggcg atgtcggctt
      301 cgtccatcgg gcggccgtac agaaacccct gcacctgacg cgctccggcg aggcgcaact
      361 ggtgcatctc ggcctccgtc tccacgccct cgatgacgtg gtccatgccg agattctgcg
      421 ccagttcgat catcgacttg atgatctcgc gcgccttttc gccgcgcccc agcgcctgca
      481 cgaacgaacg gtcgatcttg accgtgtcga cctcgacgcg gttgagatag ctgaaattgc
      541 tgtagccgga gccgaaatcg tccagcgcca tgcggcagcc gagcgcgcgc agggcggcga
      601 tattggcgcg cgcccggtcg aagtcggtca gcatggccgt ctcggtgatc tcgaactcga
      661 tgcggcgcgc gtcgaccccg gattcgcgca ccacctgcgc gatcttggcc accgccgccg
      721 gggacaggat gtcgcgcgcc gacaggttga aggccagccg cagatcgccg ggccagcgcc
      781 gcgccgcctt cagcgccttt ttcagcagca ccggcgtcag gccggtgatc aggcccgaac
      841 gctcggcggc gcggatgaac acgtcgggcg gcacctgacc cagccgggcg ttgcgccagc
      901 gcgccagcgc ctcgaaggcc agggtgcgct cgccgcgcac gtcgcgctgc ggctggaagg
      961 cgagggtcag ctccttttcg ctgtcgatgc cgcgcagcgc ctgctcgacc gccttggcgt
     1021 tgcggatcag cgcctcgtgg tgcggctcga acagcaccgc gacgccgcgg cccttttcct
     1081 tggcctcgta gagcgcgtag tcggcgcgct ccagcagctt ggacggcgcg gcggcgggct
     1141 ggttctcatc gtgcagggcg aagcccagcg aggccgacag gtgcgccgtc tccgcgtcca
     1201 gcgcataggg ccgggccaga tgttcgctga tccggtcggc gcgcgcgcgc cacgcctcgc
     1261 cgccgggcgg atcgggcagg atcagcgcga actcgtcgcc gcccagccgc gccacgaccg
     1321 cttcggggcc gcagacctcg cgcaggcggc gggcgacctc gaccagcacg cggtcgcccg
     1381 ccgcatggcc gaaaatgtcg ttgatcggct tgaagccgtc cagatcgacc agaccgaggt
     1441 aaaagcgccc gccctcgctc aggcgctgtt cggcgacgtt caggaaggcg cgccggttgg
     1501 ccagcccggt cagggcgtcg aaggcggcca gcgacagggc ctcgtcgcgg tgcgcgtcgg
     1561 cgcgcgtccg cgcgcgcacc gcctcgcgca tgctcatgcg gatcaccccg gccagaccgg
     1621 cgccgatgat cacgcccgcg aaggcgaccc agagatagtc gccgaaatgc gcgcgctcga
     1681 agcgccatgc cgcccagacg aaggcggcga ccgccgccag cgaggacggc agcgccgcgc
     1741 gcaggcgcgc ccccgaaatc gagaaggcgg gcagcaggag cagcagaaaa agcagcttga
     1801 ccggctgata cgccaggcag atgtccagca acgcattggc gatgaacagg aaccccagga
     1861 cgtggctggt gatctccagc cgcgtcatgg aggcggcgcg gcggttccac acccagaaca
     1921 ggccgcacgc gatcagggtc gcgccggaca gggcgggcag ccagcccgga tcgcccgcga
     1981 cgcggtagaa cagcgtgttg acgacgaaat aggcgatggc gaacagcagg aaggcgcgca
     2041 tattgccgca catggccgcc gccagcccct gttcttcctg acgggcttgc cgccgggacg
     2101 gcggaggatc ggcgcggcgc ggtctgaaac ggatcatgaa aggcgttttc caatgggatc
     2161 gcgccgcgcc gccgccgccg cgctttacgc ttgaagggaa aggaacgccc gaattgtctc
     2221 cgaagagggt aaacaccccg ataaggcgaa aggttaaccg cgacgatccg ccgcccttac
     2281 ggccgtttcg ggcagccgca gcagcaccgg caggacgcag acggcgacga cggcgatcat
     2341 caggcccggc gcgacgggcc aatgcgtcgt ttcgatcagc cgttgcgcgg cgtagggcgt
     2401 aaagccgccg aagagggcgg tcgccacggt ggcccccagc gccagcccgc tgagacggcc
     2461 ttcgccgggg aactgttcgg cggtggccac cgcgccgacc gcgctgacgc ccccggcgac
     2521 ggccgccaat gtcacggccc ccagcagggc ggacgccgcc gcgccgctgg ccatcaggct
     2581 gaacagggcg gtgggcagga cggcgctggc cagacagagc gcgaccagca ccggcctgcg
     2641 ccccagccgg tcggacaggg cgccgacgac cggcgtcacc gcgatcaccg ccacggccgc
     2701 gatcgtcgac agccacaggg cctgactttc cggcaaggcg ccggtcgaac tgataaaggc
     2761 cggaacatag ccgatgccga catagtaggt gatcgagccg agcgccgaga tggcgaagcc
     2821 gcgcag
//
LOCUS       DASVLL010000513         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_465633,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000513 DASVLL010000000
VERSION     DASVLL010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465633"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VF402_08515"
     CDS             complement(<1..185)
                     /locus_tag="VF402_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX7800379.1"
                     /translation="MIGRLRGLLLERGEEEALVECAGVGYIVRCGVRTLADLPDLGRE
                     VILHIDSQTREDGTRLYG"
     gene            complement(182..763)
                     /gene="ruvC"
                     /locus_tag="VF402_08520"
     CDS             complement(182..763)
                     /gene="ruvC"
                     /locus_tag="VF402_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7800380.1"
                     /translation="MKNMTRILGLDPGLRRLGWGLVDVEGARLSWVAHGVIEPPQDEA
                     LSVRLLHLFDALSAVIAEWTPDEAAIEETFVNMNPSSTLKLGHARAAAMLAPAKAGIM
                     VAEYAALDIKKSVVGAGRAEKEQVLFMVRRLLPKAPDMKADAADALACAITHAHKRKM
                     RGLAQTLTARSAPGANPPSRSEALGQLKRGEAS"
     gene            926..2452
                     /locus_tag="VF402_08525"
     CDS             926..2452
                     /locus_tag="VF402_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800381.1"
                     /translation="MNASVGIRTDSAGRAQPAAFAALALLALAVCAVGLYAGRAPILS
                     PYPEDWIGWARTTLTWSGVFAVGVVLLAVGAIGAARSLLPVRRPAARAPEAELAAPPV
                     ADAPADASDAPDIEPEAVAAPSLAAHLGVESLEPAIFHDNASDSFMSLTFGAPDLKAP
                     PSAEIIPIRPDINVAPRDPLEAALLAEAPEPAVHREAPTDLNAVITSTLRFAPETAPE
                     PVQETLPEAQPETLPETAAETLPETPAAALEADPEAEVRQAAQTALSVWPDATRAIAA
                     DELSARLARLRDDPAPDSARAFRLIATGDLNGAANALQQQANALAEAGQTSRAAEIWR
                     IFGALHMGRDDARAMHAYEQVSALDASDANIHLYLARRYQMAGDAARQPDVIRRALYV
                     ISDPDTRAELLAPYADLMLKAGDVKAGGDALEELSRLHETNAHLKPDDVGARSAYAVS
                     VARLAQAREMQGVFDQAAPLYQKAHRVFSDLSAMKPDHPGLRALADKALQDARRFQSA
                     "
     gene            complement(2469..>2891)
                     /locus_tag="VF402_08530"
     CDS             complement(2469..>2891)
                     /locus_tag="VF402_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7800382.1"
                     /translation="GEAGKGFAVVASEVKSLAGQTAKATTEISDKIAQIQQTTARAVS
                     AIESITGTITDINTASNHIANTVDQQNGATQEIVHAVAQASAGAGEVSSNIASMAHSA
                     EQTGSAAAQVLGASSQLARQADTLRQEMTRFLGRIRAA"
BASE COUNT          411 a         1035 c          989 g          456 t
ORIGIN      
        1 ccgtaaagcc gcgtgccgtc ctcgcgcgtc tggctgtcga tatgcaggat cacttcgcgg
       61 ccaaggtcgg gtaaatcggc caatgtgcgc acgccgcagc gcacgatata gccgacgccc
      121 gcgcattcga cgagcgcctc ctcctcgccg cgctccagca acagtccgcg caaacgtccg
      181 atcacgatgc ttcgcctctt ttaagttgcc ctaacgcttc gcttcttgag ggcgggttgg
      241 cgccgggagc gcttcgcgcg gtcagggtct gagccaggcc gcgcatcttc ctcttatgcg
      301 catgcgtgat cgcgcaggcc agcgcgtcgg cggcgtcggc cttcatgtcg ggcgccttgg
      361 gcagcaggcg ccggaccatg aacaagacct gctccttctc ggcgcggccc gcgccgacca
      421 ccgacttctt gatgtcgagc gcggcgtatt cggcgaccat gatcccggcc ttcgcgggcg
      481 ccagcatggc ggcggcgcgc gcgtggccca gtttcagcgt cgaggacgga ttcatgttga
      541 cgaaggtttc ctcgatcgcc gcctcgtccg gcgtccactc ggcgatcacc gcgctcaggg
      601 cgtcgaacag gtgcagcagc cgcaccgaca gcgcctcgtc ctgcggcggt tcgatcacgc
      661 cgtgcgcgac ccacgacagg cgcgcgccct cgacgtcgac aagcccccag ccgagcctgc
      721 gcaggccggg atcgagaccg agaatccgcg tcatgttctt catatgttcg gttctatccg
      781 caagttcggc gctccgcaag cgggattggc cggaaagttg catacgcccg caaggatggg
      841 cgcgacacgc ccattatggc gcatcggctt gccatatggc cgaagctgag gttaatgttt
      901 tctaaacggc aatgcggggg ccaccatgaa cgccagcgtc gggatcagga cggacagcgc
      961 cgggcgcgca caaccggcgg ccttcgccgc gctggcgctg ctggccctgg cggtgtgcgc
     1021 ggtcgggctt tatgcggggc gcgcgccgat tctcagcccg tacccggaag actggatcgg
     1081 ctgggcgcgc accaccctga cctggtccgg cgtgttcgcc gtcggcgtcg tgctgctggc
     1141 cgtcggcgcg atcggcgcgg cgcgcagcct tttgcccgtc cggcggcccg ccgcgcgggc
     1201 gcccgaggcc gagcttgcgg cgccgcctgt cgcggacgcg cccgccgacg cttcggacgc
     1261 gcccgatatc gagccggaag ccgtcgccgc gccgtctctg gccgcgcatc tgggcgtcga
     1321 gtcgctggag cccgccatct ttcacgacaa cgcgtccgac agcttcatga gcctgacctt
     1381 cggcgcgccc gacctcaagg cgccgccgtc cgccgagatt attcccatcc gcccggacat
     1441 caacgtcgcg ccgcgcgatc cgctggaagc cgcgctgctg gccgaggcgc cggagcccgc
     1501 cgtgcatcgc gaggcgccca ccgacctgaa cgccgtcatc acctcgaccc tgcggttcgc
     1561 gccggaaacg gcgccggagc cggttcagga gacgctgccg gaggcgcagc cggaaacttt
     1621 gcccgaaacg gcggctgaaa ccttgcccga aacgcccgcc gccgctcttg aggccgatcc
     1681 tgaggccgag gtgcgtcagg ccgcgcagac ggcgctgtcg gtctggcccg acgccacacg
     1741 cgccatcgcc gccgacgagt tgagcgcgcg tctggcgcgg ctgcgcgacg atccggcgcc
     1801 cgacagcgcg cgcgccttcc gcctgatcgc caccggcgat ctgaacggcg ccgcgaacgc
     1861 cttgcagcag caggcgaacg ccctggccga agccgggcag accagccgcg ccgccgaaat
     1921 ctggcgcatc ttcggcgcgc tgcacatggg ccgcgacgac gcgcgcgcca tgcacgccta
     1981 tgagcaggtg tccgcgctgg acgcctccga cgccaatatc catctctatc tggcgcgccg
     2041 ttatcagatg gcgggcgacg ccgccaggca gcccgacgtc atccgccgcg cgctctacgt
     2101 gatcagcgac ccggacacgc gcgccgagct gctggcgccc tatgccgacc tgatgctgaa
     2161 agccggggac gtcaaggcgg gcggcgacgc gctggaggag ctgagccgcc tgcacgagac
     2221 gaacgcgcat ctgaagcccg acgatgtcgg cgcgcgctcg gcctatgcgg tcagcgtggc
     2281 gcggctggcg caggcgcgcg aaatgcaggg cgtcttcgat caggccgcgc ctctgtacca
     2341 gaaggcgcac cgcgtctttt ccgacctgtc ggccatgaag cccgatcatc cgggcctgcg
     2401 cgctttggcc gacaaggccc tgcaggacgc gcggcgcttc cagagcgcct gacgcgtccg
     2461 cgactcgcct aggccgcgcg gatgcggccg aggaagcgcg tcatttcctg acgcagggtg
     2521 tcggcctgcc gggccagttg cgacgaggcg cccagcacct gcgcggcggc cgagccggtc
     2581 tgttcggcgg aatgggccat gctggcgatg ttggaggaca cctcgcccgc gcccgccgaa
     2641 gcctgcgcga cggcgtggac gatttcctgc gtcgcgccgt tctgctgatc gacggtgttg
     2701 gcgatgtggt tgctggccgt attgatgtcc gtgatcgtgc cggtgatgct ctcgatggcc
     2761 gagacggcgc gcgccgtggt ctgctggatc tgggcgatct tgtcggagat ttcggtggtc
     2821 gccttggcgg tctggccggc cagcgacttg acctcggagg cgacgacggc gaagcccttg
     2881 cccgcctcgc c
//
LOCUS       DASVLL010000514         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_144390,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000514 DASVLL010000000
VERSION     DASVLL010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144390"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VF402_08535"
     CDS             <1..1260
                     /locus_tag="VF402_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7800383.1"
                     /translation="PGTVRRLNALGAPFDKTPDGGFVLNREAAHGLARVARVGGDLAG
                     KKILAAVVAAAQKAAHIEIWDDAHALGLIRSDVRTVIGARVRRGDRLIDVYAPAVILA
                     TGGSGGLYAVTTNPSALRGEGMAMAALAGAGIADPEFVQFHPTALNVGRDPAPLATEA
                     LRGEGAVLIDAQGERFAFDDHPDGELAPRDIVARAVHKANLHRGGAFLDATHAIGAHF
                     DTEFPTVFAACMAEGIDPRVQPMPVAPAAHYHMGGVRADLDGRTSLKGLYAVGECAAT
                     GVHGANRLASNSLLEAAAFGERTGKAACAHLPDAFAPDAAHAIVPAVPPELPPQDLRA
                     LRDAMSRDAGMVRSEAGLRELLALLDRLDALYPGALALVAARLIATCALQRRESRGSH
                     YRSDFPQTEKTGERTYTDWNAVNKEVV"
     gene            1260..2108
                     /gene="nadC"
                     /locus_tag="VF402_08540"
     CDS             1260..2108
                     /gene="nadC"
                     /locus_tag="VF402_08540"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7800384.1"
                     /translation="MINPGLPDILIEPVVRAALLEDLGRAGDVTADAVIPPKARFQAE
                     FRARGHGVSAGIDCARLAMSLMDPQIRFETVLPDGSVVKPGDVIARIEGKARAILAAE
                     RTALNLLCHLSGVASLTARYVAALDGLNARIVCTRKTLPSLRALQKHAVRCGGGYNHR
                     YGLDDAILIKDNHIAICGGVEAAMQRAKAGVGHTMKIEIEVDSIAQLKVALPFKPDVV
                     LLDNFTPDMFAQAVKLAAGKTLLEASGGVNLDTVRAIAETRVDVISVGALTHSAPNFD
                     IGLDAV"
     gene            complement(2205..>2405)
                     /locus_tag="VF402_08545"
     CDS             complement(2205..>2405)
                     /locus_tag="VF402_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7800385.1"
                     /translation="HSLVGAIWFGVTARLTAHFGLGAAAQWALRSLGVLLAVVAAFVF
                     DALVDKPLSQRVSRLSFVKGGV"
BASE COUNT          365 a          878 c          825 g          337 t
ORIGIN      
        1 cccggcacgg tgcgccgcct caacgccctc ggcgcgccgt tcgacaagac gccggacggc
       61 ggcttcgtgc tgaaccgcga ggccgcgcac ggcctggccc gcgtggcgcg cgtcggcggc
      121 gatctggcgg gcaagaagat cctcgccgcc gtggtcgcgg cggcgcaaaa ggccgcgcat
      181 atcgagatct gggacgacgc ccacgcgctc ggcctgatcc ggtcggacgt caggaccgtg
      241 atcggcgcgc gggtccggcg cggggaccgg ctgatcgacg tctacgcgcc cgccgtcatt
      301 ctggccaccg gcggttcggg cgggctttac gccgtcacca ccaatccgtc cgccttgcgc
      361 ggcgaaggca tggcgatggc ggcgctggcg ggcgccggga tcgccgaccc ggagttcgtg
      421 cagttccatc cgaccgcgct gaacgtcggc cgcgacccgg cgccgctggc caccgaggcc
      481 ctgcgcggcg aaggcgccgt actgatcgac gcgcagggcg agcgcttcgc cttcgacgat
      541 cacccggacg gcgagctggc gccgcgcgac atcgtggcgc gcgcggtgca taaggcgaac
      601 cttcatcgcg gcggcgcctt cctcgacgcc acgcacgcca tcggcgcgca tttcgacacg
      661 gaatttccga ccgtcttcgc cgcctgcatg gccgagggga tcgacccgcg cgtccagccc
      721 atgccggtcg cgcccgccgc gcactaccat atgggcggcg tccgcgccga tcttgacggc
      781 cgcacgtcgc tgaagggcct ctatgcggtc ggcgaatgtg cggccaccgg cgtccacggc
      841 gccaaccgcc tcgcctccaa cagccttctg gaagccgccg ccttcgggga gcgcacgggc
      901 aaggcggcgt gcgcccatct gccggacgcc ttcgcgccgg acgcggccca tgcgatcgtc
      961 cccgccgttc cgccggaact gccgccccaa gacctgcgcg ccctgcgcga cgccatgagc
     1021 cgcgacgcgg gcatggtgcg ctctgaggcc gggctgcgcg agctgctggc cctgctggac
     1081 cggctggatg cgctttatcc cggcgccctg gcgctggtcg cggcgcggct gatcgcgact
     1141 tgcgccttgc aaagacgcga gagtcgcggt tctcattacc gcagcgactt cccgcagacg
     1201 gaaaagacgg gcgagcgcac ctatacggac tggaacgctg tcaacaagga agtcgtctga
     1261 tgatcaatcc cggcctgcct gacatcctga tcgaacccgt cgtgcgcgcc gcgctgctcg
     1321 aagacctggg ccgcgcgggc gatgtgaccg ccgacgcggt gatcccgccg aaggcgcgct
     1381 ttcaggccga gttccgggcg cgcggccacg gcgtcagcgc cgggatcgac tgcgcgcggc
     1441 tggccatgag cctgatggac ccgcagattc gcttcgagac ggttttgccc gacggcagcg
     1501 tggtgaagcc cggcgacgtg atcgcgcgca tcgagggcaa ggcgcgcgcc atcctggccg
     1561 ccgaacgcac ggcgctgaat cttctgtgcc atctgtcggg cgtggccagc ctgaccgcgc
     1621 gatacgtcgc cgcgctggac gggctgaacg cgcgcatcgt ctgcacgcgc aagaccctgc
     1681 cttccttacg cgcgctgcaa aagcacgcgg tgcgctgcgg cggcggctac aaccaccgct
     1741 acggcctcga cgacgccatc ctgatcaagg acaaccacat cgccatctgc ggcggcgtcg
     1801 aagcggccat gcagcgcgcc aaggcgggcg tcggccacac catgaagatc gagatcgagg
     1861 tcgattccat cgcccagctc aaggtcgccc tgcccttcaa gccggacgtc gtcctgctcg
     1921 acaacttcac gcccgacatg ttcgcgcagg ccgtgaagct ggcggcggga aagaccctgc
     1981 tggaagcgtc cggcggcgtc aatctcgaca ctgtgcgcgc catcgccgaa acccgcgtcg
     2041 acgtcatcag cgtcggcgcc ctgacccact cggcgccgaa tttcgacatc gggctggacg
     2101 cggtctgatc gaccggcgga gggcttccgc cgggtgcgac gccttggggt cgcggggcat
     2161 tcgggacggt ctgcgcgccg gcggcctgac gatccgcaat ccgttcagac gccgccctta
     2221 acgaagctca accgcgacac gcgctggctc agcggcttgt cgaccagcgc gtcgaagacg
     2281 aaggcggcga cgacggccag cagcacgccg aggctcctta aggcccactg cgcggcggcg
     2341 ccgaggccga aatgcgcggt cagtctggcc gtaacgccga accagatcgc gccgaccagc
     2401 gaatg
//
LOCUS       DASVLL010000515         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_409642,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000515 DASVLL010000000
VERSION     DASVLL010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409642"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2093
                     /locus_tag="VF402_08550"
     CDS             <1..>2093
                     /locus_tag="VF402_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800386.1"
                     /translation="NMNHASIAPGVPFMAGCYDWSTGCSPTQMGERLDTTGRIFDRRE
                     GTQDLSFNLKWDVNDRLSTNFDVQYIKASQNSYDIMLSPHTQADIVYSTDGDGVPEAS
                     FAPDAGNNYASGFLGNMHNWYYGFVQDHHDHSTANELAVRADAEYRFGGGGWLDSLRV
                     GVRRAERKQQVDYSAFNWAGLYANWGCDAAMFSIDNGPGGYVADGSCPSPTNPNNATF
                     NGYKPGLSELASFGGDFYNGHVLANTPMPFPNVGRLGSEQGLIDELSVSALNNGVVGQ
                     GWTPICERATDVSGSCYTQAEMLYVDEVTNAAYLELKFGGSDKTIFGSSVTLSGNVGV
                     RYVQTKDTSDGGIAFPLQDWYNNANAQPCNKPITDPHQVTNISCWLTPELLAYSNSGS
                     ALQSYGGEHDNWLPSLNLKFGLTDKQLIRFAASRALSRPDMGQLRNYLSVAAPAIDTS
                     DTSPYIVWNDPNAPHTAANVKGYNFVFTGSSGNPGLQPITADQFDLSYENYFAPSGSF
                     TFDLFYKRFNSSLGLGKYARDLSNNGVTETVIVTAGANTNDGGNLRGFEAAYQQFFDF
                     LPGLWSGLGVQANYTHVSQNGINNTNLANFPGYDSGSTIAYGGGVDYGTAVIDSHRLA
                     GISDDTYNLVALYEKGPVAARLAYNYRSKYLVNNLDAIIGLPVFEKGAGYLDASIRYQ
                     INDHVEISLEGTNLTG"
BASE COUNT          406 a          696 c          637 g          354 t
ORIGIN      
        1 aatatgaatc acgcctcgat cgcgccgggc gtgccgttca tggcgggctg ctatgactgg
       61 tcgacgggtt gttcgccgac ccagatgggc gaacgtctgg acaccacggg ccgcatcttc
      121 gatcgccgcg aaggcacgca agacctgtcg ttcaacctga aatgggacgt caacgaccgg
      181 ctgtcgacca atttcgacgt gcagtacatc aaggcgtcgc agaacagcta cgacatcatg
      241 ctgtcgccgc acacccaggc cgacatcgtc tattcgaccg acggcgacgg cgtgcctgag
      301 gcctcgttcg cgccggacgc gggcaacaat tacgccagcg gcttcctcgg caacatgcac
      361 aactggtact acggcttcgt gcaggatcac cacgatcatt ccaccgccaa cgaactggcc
      421 gtgcgcgcgg acgccgaata ccggttcggc ggcggcggct ggctcgacag cctgcgcgtc
      481 ggcgtgcggc gcgccgagcg caagcagcag gtcgactatt cggccttcaa ctgggcgggc
      541 ctgtacgcca actggggctg cgacgcggcc atgttctcga tcgacaacgg gccgggcggc
      601 tatgtcgccg acggatcgtg tccgtcgccc accaatccga acaacgcgac cttcaacggc
      661 tataagccgg gcctgtcgga actggccagc ttcggcggcg acttctataa cggccatgtg
      721 ctggccaaca cgccgatgcc gttccccaat gtcggccgtc tcggctccga acaggggctg
      781 atcgacgagc tgagcgtgtc cgcgctgaac aacggcgtgg tggggcaggg ctggacgccg
      841 atctgcgagc gcgcgacgga tgtgtcggga agctgctaca cccaggcgga aatgctgtac
      901 gtcgacgagg tgaccaacgc ggcctatctg gagctgaaat tcggcggttc ggacaagacg
      961 atcttcggct cgtccgtgac gctgagcggc aatgtcggcg tgcgctacgt ccagaccaag
     1021 gacacctcgg acggcggcat cgccttcccg ttgcaggact ggtacaacaa cgccaacgcg
     1081 caaccgtgca acaagccgat caccgacccg catcaggtga ccaacatctc gtgctggctg
     1141 acgccggagc tgctggccta ctccaattcc ggttcggcgc tgcaatccta tggcggcgag
     1201 catgacaact ggctgcccag cctgaacctg aagttcggcc tgaccgacaa gcaactgatc
     1261 cgcttcgccg cctcgcgcgc cctgtcgcgg ccggacatgg gccagttgcg caactacctg
     1321 tcggtggccg cgccggccat cgacacgtcg gacacctcgc cctacatcgt ctggaacgat
     1381 ccgaacgcgc cgcacaccgc cgccaatgtg aagggctata acttcgtctt caccggctcg
     1441 tccggcaatc cggggctgca accgatcacg gcggaccagt tcgacctgtc gtatgaaaac
     1501 tacttcgcgc cttcggggtc gttcaccttc gacctgttct acaagcgctt caactccagc
     1561 ctgggcctcg gcaaatacgc gcgcgacctg agcaataacg gggtgacgga gacggtgatc
     1621 gtgaccgccg gggccaacac caacgacggc ggcaacctgc gcggcttcga ggccgcctac
     1681 cagcagttct tcgacttcct gccggggctg tggtcggggc tgggcgtgca ggccaactac
     1741 acccatgtca gccagaacgg catcaacaac accaatctgg ccaacttccc cggctatgac
     1801 agcggttcga ccatcgccta cggcggcggc gtggactacg gcacggcggt gatcgattcg
     1861 caccgtctgg ccgggatctc ggacgacacc tacaacctgg tcgccctgta cgaaaagggg
     1921 ccggtggcgg cgcgtctggc ctataattac cgctcgaaat atctggtgaa caatctggac
     1981 gccattatcg gcctgccggt gttcgagaag ggggcgggct atctcgacgc ctcgatccgc
     2041 taccagatca acgaccacgt cgaaatctcg ctggaaggca ccaacctgac cgg
//
LOCUS       DASVLL010000516         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_88546, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000516 DASVLL010000000
VERSION     DASVLL010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88546"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VF402_08555"
     CDS             <1..1020
                     /locus_tag="VF402_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEX7800387.1"
                     /translation="YDFDPVRNPFMMERMGINAVFNAGAMLFDGRYVVVARVEGDDRK
                     SFFAVAESPNGIDNFRFWPRPVVLPQTEEPDTNVYDMRLTRHEDGWIYGLFCTERKDR
                     SQPRDLSAATAQCGIARTKDLVDWERLPDLKTRSGQQRNVVLHPEFVDGQYALYTRPQ
                     DGFISVGSGGGVGWGLCADMTNAVVEAETIVDDKVYHTIKEVKNGQGPAPIRTGQGWL
                     HLAHGVRNTAAGLRYVLYLFMTELARPWVVSHAPGGHFLAPQGDECVGDVSNVVFSNG
                     WIEDQGKVFIYYASSDTRLHVATSSVAQLTDYCLNTPPDPLTSAGCVAQRLDLIARNA
                     AFPAR"
     gene            1017..>1678
                     /locus_tag="VF402_08560"
     CDS             1017..>1678
                     /locus_tag="VF402_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX7800388.1"
                     /translation="MRRALDTLRLEARAEADAILRWWRVHAPDPGGGFYGEIDASGRP
                     VAEAPRAAILNARLLWFFSASGEAALARRAADYLNAHLFDREQGGVYWSVDAAGAPLD
                     TKKQTYAQAFALYGFAEYYAATGDAAALAAARGLRDLIETRFRDAERGGYIEALTRDG
                     APLADPRLSDKDMDAPKTMNTHLHVLEAYTRLHQVAPDAATQAALRGAAQVFLDRFVG
                     PEG"
BASE COUNT          264 a          541 c          609 g          264 t
ORIGIN      
        1 tacgatttcg atccggtccg caatccgttc atgatggagc ggatggggat caacgccgtg
       61 ttcaacgccg gcgccatgct gttcgacggc cggtatgtcg tcgtggcgcg cgtcgagggc
      121 gacgaccgca agtcgttctt cgccgtggcg gaaagcccga acgggatcga caatttccgc
      181 ttctggccgc gtccggtggt cctgccgcag acggaggagc cggatacgaa cgtttacgac
      241 atgcgcctga cccggcatga ggacggctgg atctacggcc tgttctgcac cgagcgcaag
      301 gataggagcc agccgcgcga cctgagcgcg gcgacggcgc aatgcgggat cgcgcgcacg
      361 aaagacctcg tggactggga gcggctgccg gacctgaaga cccgctccgg ccagcagcgc
      421 aacgtcgtgc tgcaccccga attcgtcgac ggccagtacg cgctctatac ccgcccgcag
      481 gacggcttca tctcggtcgg ctccggcggc ggcgtcggct gggggctgtg cgccgacatg
      541 acaaacgcgg tcgtcgaggc ggaaaccatc gtcgacgaca aggtctatca cacgatcaag
      601 gaggtgaaga acgggcaggg accggcgccg atccgcaccg gccaaggctg gctgcatctg
      661 gcgcacggcg tgcgcaacac ggcggcgggg ctgcgttatg tgctctacct gttcatgacc
      721 gagctggcgc gcccgtgggt cgtcagccat gcgccgggcg ggcatttcct ggcgccgcaa
      781 ggcgacgagt gcgtcgggga cgtgtcgaac gtcgtgttct cgaacggctg gatcgaggat
      841 cagggcaagg tcttcatcta ttacgcctcg tccgacacgc gcctgcatgt ggcgacctcg
      901 tcggtcgcgc aactgacgga ctactgcctg aacacgccgc cggacccgct gacctcggcg
      961 ggttgcgtcg cgcagcgcct cgacctgatc gccaggaatg cagcgtttcc ggcgcgatga
     1021 gacgggcgct ggacacgctg cggctggagg cccgcgcgga ggcggacgcc atcctgcgct
     1081 ggtggcgcgt ccatgcgccg gaccccggcg gcggctttta cggcgagatc gacgcgtccg
     1141 gccggcctgt tgccgaagcg cccagggcgg cgatcctcaa cgcgcgcctg ttgtggttct
     1201 tttccgccag cggggaggcg gcgctggcgc ggcgcgcggc ggactatctg aacgcgcatc
     1261 tgttcgaccg tgaacagggc ggcgtttact ggagcgtcga cgcggcgggc gcgccgctgg
     1321 atacgaagaa gcagacctac gcgcaggcct tcgcgcttta cggcttcgcg gaatattacg
     1381 ccgcgacggg cgacgcggcg gcgctggcgg cggcgcgggg tttgcgcgac ctgatcgaga
     1441 cgcgcttccg ggacgcggag cgcggcggtt atatcgaggc gctgacgcgc gacggcgcgc
     1501 ctctggccga tccgcgcctg tccgacaagg acatggacgc gcccaagacg atgaacaccc
     1561 acctgcatgt gctggaagcc tatacgcggc tgcatcaggt cgcgccggac gcggcgacgc
     1621 aggcggcgct gcgcggcgcg gcgcaggtgt ttctggaccg ttttgtcggc ccggaagg
//
LOCUS       DASVLL010000517         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_33983, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000517 DASVLL010000000
VERSION     DASVLL010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33983"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /gene="infB"
                     /locus_tag="VF402_08565"
     CDS             <1..1621
                     /gene="infB"
                     /locus_tag="VF402_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7800389.1"
                     /translation="LDRGRGSVATVLVKRVSESDVLEGFIDAEDHEDDTQPRPPVVAV
                     MGHVDHGKTSLLDAMRKTDTAGGEAGGITQHIGAYQVRVASGERVTFLDTPGHAAFSA
                     MRARGANVTDIVVLVVAADDGVMPQTVEAINHARAAGTPMIVAINKIDKPDAKPQNII
                     NELLQYEVVVESLGGETQVVEVSAKTGQGLDSLIEAILLQAEVLDLRANPDSTAEGVV
                     IEAKLDKGRGPVATVLVKRGTLKRGDNVVAGSAYGRVRALINERDEQLNEAGPSMPVE
                     ILGLNGAPDPGEAIAVVESEARAREITEYRERVRREKTMAPVGAVSLSDMMSRLAANK
                     AKEVQLIVKSDVQGSAEAIIGSLDKLGTDEVRARVIHSGAGAITESDVQLAKGSNAPI
                     IGFNVRAGKQARDLAEREGVEIRYYAIIYDLIDDIKGVMSGLLAPIQRETFLGNAEVL
                     EVFDISKVGRVAGCRVTEGRVQKGARVRILRDNVVIQEMGVLTTLKRFKDEVNEVVVG
                     QECGMSFGPFQDIKQGDFIECFTVEEIKRTL"
     gene            2347..3225
                     /gene="purU"
                     /locus_tag="VF402_08570"
     CDS             2347..3225
                     /gene="purU"
                     /locus_tag="VF402_08570"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX7800390.1"
                     /translation="MPDTSSFDPSHHVLIIKCPDTRGIVAAVSGYLNDNDISIVESNQ
                     FNDSEGDMFYVRVVFRQAGPKMPPMETLRDGFKPIAHRFSMDWSIHNLSIKPRVVIAV
                     SKFGHCLYELLHRWQSGQLPVEIACVVSNHEDMRSFVEWNGLPYVHLPVDKANKAGQE
                     AALLTLIEDQRADLVVLARYMQILSDDLARRLEGRCINIHHSFLPSFKGAKPYHQAHA
                     RGVKIIGATAHYVTSDLDEGPIIEQDVQRVHHGMTPEQMVVIGQGIEARVLARAVTWH
                     AERRVIINGGKTVVFS"
BASE COUNT          660 a         1029 c         1038 g          610 t
ORIGIN      
        1 gctcgaccgg ggtcggggct ctgtggccac ggtcctggtc aagcgcgtgt cggaatcgga
       61 cgttctggaa ggcttcatcg acgccgagga ccacgaggac gacacccagc cgcgtccgcc
      121 ggtcgtggcc gtcatgggcc acgtcgacca cggcaagacc tccctgctcg acgcgatgcg
      181 caagaccgac acggcgggcg gcgaagcggg cggcatcacc cagcacatcg gcgcctatca
      241 ggtgcgggtg gcgtcgggcg agcgcgtcac cttcctcgac acgccgggcc acgcggcctt
      301 ctcggccatg cgcgcgcgcg gcgccaacgt caccgacatc gtggtgctgg tggtggccgc
      361 cgacgacggc gtcatgccgc agacggtcga ggcgatcaac cacgcccgcg ccgccggcac
      421 gccgatgatc gtggcgatca acaagatcga caagccggac gccaagccgc agaacatcat
      481 caacgagctg ctgcaatacg aggtcgtggt cgaaagcctc ggcggcgaaa cgcaggtggt
      541 cgaggtttcg gccaagaccg gtcagggcct cgacagcctg atcgaagcca tcctgctgca
      601 ggccgaggtt ctggacctgc gcgccaaccc cgacagcacc gccgagggcg tggtgatcga
      661 ggccaagctg gacaagggcc gcggcccggt ggcgaccgtg ctggtcaagc gcggcacgct
      721 caagcgcggg gataacgtgg tggcgggttc ggcctatggc cgcgtgcgcg ccctgatcaa
      781 cgagcgcgac gagcaactga acgaagccgg tccgtccatg ccggtcgaga tcctcggcct
      841 caacggcgcg cccgatccgg gcgaggccat cgccgtggtc gaatccgaag cccgcgcgcg
      901 cgagatcacc gaatatcgcg aacgcgtccg ccgcgaaaag accatggccc cggtcggcgc
      961 cgtgtcgctc agcgacatga tgtccaggct ggcggccaac aaggccaagg aagtgcaact
     1021 gatcgtcaag tccgacgtgc aggggtccgc cgaagccatt atcggctcgc tggacaagct
     1081 gggcaccgac gaggtgcgcg cccgcgtcat ccattccggc gccggggcga tcaccgaatc
     1141 ggacgtgcaa ctggccaagg gctcgaacgc gccgatcatc ggcttcaacg tccgcgccgg
     1201 caagcaggcg cgcgacctgg ccgagcgcga gggcgtggag atccgctact atgcgatcat
     1261 ctacgacctg atcgacgaca tcaagggcgt catgtcgggc ctgctggcgc cgatccagcg
     1321 cgaaaccttc ctcggcaacg ccgaggtgct ggaggtgttc gacatctcca aggtcggccg
     1381 cgtcgcgggc tgccgcgtca ccgagggccg ggtgcagaag ggcgcgcgcg tccgcatcct
     1441 gcgcgacaac gtcgtcattc aggaaatggg cgtcctcacc acgctcaaac gcttcaagga
     1501 cgaggtcaac gaggtggtcg tcggccagga atgcggcatg agcttcggcc cgttccagga
     1561 catcaagcaa ggcgatttca tcgaatgctt cacggtcgag gaaatcaagc ggacgcttta
     1621 acggttcgct taaactgaac ttatgtgatg cggggtcaat cggccccgca tcttttttgt
     1681 cacaacctcg aatcggtgct aactcgccat atagattaat ttggttgaga gaagcgccag
     1741 aaatgccaat tcagtttttc ccgcgagcag ggcaaatcct gatttgcgac ttttctgatt
     1801 tcaaagaacc tgaaatgtgc aaaatcaggc cggttatcat tgtatcgcca cggctgcctc
     1861 accgcagtga gatcgttacg gttgtcccca taagcacgac ggcgcctatc catgattttc
     1921 cttacgcagt aaggctttcc aaaaattacc atcctttgga ggcggatgat ttaccctgct
     1981 gggccaaatg tgacatgatc atgaacattg cccgacggcg actggacggc tttaaggtcg
     2041 gcaaaagaag gtgggaaacg cctcaagcga gcggagatga tttacgagcg gttagaatcg
     2101 gcgtgataca tggtttaggc atgggcatct tgctaaagaa cgccgatcag actatataga
     2161 cgcggtatcc cggttatcgg gctttaagac ctagtctcta ggcaggctgt tcaatgagcg
     2221 atgacaagct tcgagcagtc tagatattgt cgccgtccct tagccccgct tcggcggggc
     2281 tttgtgtttc cggcaaacgc gaaaatgcgc ctatgacggc attgaacggg atgatatagg
     2341 cccttcatgc ctgacacatc ctctttcgac ccctcccacc acgttctgat catcaagtgc
     2401 ccggacacgc gcggcatcgt cgcggcggtg tcgggttatc tgaacgacaa cgacatttcc
     2461 atcgtcgagt ccaaccagtt caatgattcc gaaggcgaca tgttctatgt ccgcgtcgtg
     2521 ttccggcagg ccgggccgaa aatgccgccg atggagactt tacgcgacgg tttcaagccg
     2581 atcgcgcacc gtttcagcat ggactggtcg atccacaacc tgtcgatcaa gccgcgcgtg
     2641 gtgatcgccg tgtcgaagtt cggccattgc ctgtacgagc tgctgcaccg ctggcagtcg
     2701 gggcagttgc cggtcgagat cgcctgcgtc gtgtcgaacc acgaggacat gcgctccttc
     2761 gtcgaatgga acggcctgcc ttatgtccat ctgccggtcg acaaggcgaa caaggccggg
     2821 caggaagccg cgctgctgac gctgatcgag gaccagcgcg ccgacctcgt ggtgctggcg
     2881 cgctacatgc agattctcag cgacgatctg gcgcggcggc tggaggggcg ctgcatcaac
     2941 atccaccact ccttcctgcc gtcgttcaag ggcgccaagc cgtaccatca ggcgcacgcg
     3001 cgcggcgtga agatcatcgg cgccaccgcc cactacgtca cctctgacct cgacgaaggc
     3061 ccgatcatcg agcaggacgt gcagcgcgtc caccacggca tgacgcccga acagatggtg
     3121 gtgatcggtc agggcatcga ggcgcgcgtg ctggcccgcg ccgtgacgtg gcacgccgag
     3181 cgccgcgtca tcatcaacgg cggcaagacg gtcgtctttt cctagagcgc ttttcgatct
     3241 gattgcatcg gatttgcgct ctcggttttt gtctgtcgct cttccttatc caaaaagtgg
     3301 ctccactttt tggggaaacg ctctaggttg tggtgac
//
LOCUS       DASVLL010000518         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_466375,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000518 DASVLL010000000
VERSION     DASVLL010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466375"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..846)
                     /locus_tag="VF402_08575"
     CDS             complement(25..846)
                     /locus_tag="VF402_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7800391.1"
                     /translation="MHLQDRWKRIIDELNTDRVTSVDELTERLGVSPATIRRDLNELH
                     ELGHLLRVRGGAMRAEGAVYAARGEERRSGLQSQPAFADSVITNAAAKRAIARLALDY
                     ITPGLSMIVDGGTTTFMMASMMADDAYHVLTTSVPILQCLFERPRIKVTVPGGELFRD
                     QGIILNPYEDGILQHFAARRMIIGCQALTRNGLMQTDTLLVQSERRLIERAAEVIVLA
                     DSSKIDAPASLSVCPLKDIDIVITDDKIDPNAVRWLNAHDIEVKIARLDADAAAA"
     gene            complement(1035..2453)
                     /locus_tag="VF402_08580"
     CDS             complement(1035..2453)
                     /locus_tag="VF402_08580"
                     /inference="COORDINATES: protein motif:HMM:NF044822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX7800392.1"
                     /translation="MKRERRSRNGPNCLVFDAGRTYAKVSVVTPSGQVLAQRRTPTPT
                     FATPLYTAIDTDALFTWLLAQLRTFSDFGIDRIIPVAHGAACAFLDDGLNLLQPIQDY
                     ESAIPPAFARAYARRRPGFDETLSPPLPKGLNLGAQIWWHQHRDPALFARARWILPYP
                     QYWAWRLSGALHAEITSLGCHSDLWAPRRNDFSSLAHDQDWSRRFPALRKAWESAGPL
                     KADIARLTGLPATTRVCVGLHDSGAALAALLGGWQQDDDLPALLSTGTWFIAMAPGGA
                     LDHLQAERDCMGAVDVFGNAVPCARFMGGRAFEMITEGGCASALDVDAATLAEVMHEA
                     ALALPSFLDAGGPYPGLRGEIRGLRKDTPRTRAALGMLYQALLATTCLDMIQCGRTLL
                     IEGMAAGNGVLSGLIAALHDGPVLCNTGPSGVTLGAAALAFLGERPMPRLIHRQVTPL
                     LPAEMRDYQRIWREAISAEIAA"
BASE COUNT          410 a          856 c          958 g          453 t
ORIGIN      
        1 ctgaggcgga catgccgccg ggccttacgc cgccgccgcg tcggcgtcga gtctggcgat
       61 cttgacctcg atgtcgtgcg cgttgagcca gcgcacggcg ttggggtcga tcttgtcgtc
      121 ggtgatcacg atgtcgatgt ccttgagcgg gcacaccgac agggaggcgg gcgcgtcgat
      181 cttgctggag tcggccagca cgatcacctc ggcggcgcgc tcgatcaggc ggcgctcgga
      241 ctgcaccagc agggtgtcgg tctgcatcag cccgttgcgc gtcagcgcct gacagccgat
      301 gatcatgcgg cgcgcggcga agtgttgcag gatgccgtcc tcatacgggt tgaggatgat
      361 gccctgatcg cggaacagct cgccgcccgg cacggtgacc ttgatgcgcg ggcgctcgaa
      421 caggcattgc aggatcggca ccgaggtggt cagcacgtga taggcgtcgt cggccatcat
      481 cgacgccatc atgaaggtcg tcgtgccgcc gtcgacgatc atcgacaggc ccggcgtgat
      541 gtagtcgagc gccagcctgg cgatggcgcg cttggcggcg gcgttggtga tcacgctgtc
      601 ggcgaaggcc ggttgcgatt gcaggcccga acggcgctcc tcgccgcgcg cggcgtacac
      661 cgcgccctcg gcccgcatgg cgccgccgcg cacgcgcaac aggtggccca gctcgtgcag
      721 ctcgttgaga tcgcgccgga tggtggcggg cgatacgccc agccgttcgg tcagttcgtc
      781 gacggaggtg acgcggtcgg tgttcagctc gtcgataatg cgtttccagc ggtcttgcag
      841 atgcatggtt cggtcctgtt ggcgccgata tttaataacg gcgattcgta aagaatcggt
      901 tgtaacgccg cacggaattg cgcgcaagac gcgactctat gattgctcat gatcgattct
      961 ataggaggaa tccatgcgcc tgtcgagcgt tgtcacgccg cacgcgcggc gcggcgttcg
     1021 ggatcgcggt aagatcaggc cgcgatctcg gcggagatgg cttcgcgcca gatccgctga
     1081 tagtcgcgca tctcggcggg cagcagcggc gtcacctgac ggtggatcag gcgcggcatg
     1141 ggccgttcgc ccaggaaggc cagcgcggcg gcgcccaggg tgacgccgct ggggccggtg
     1201 ttgcacagca ccgggccgtc gtgcagcgcc gcgatcaggc ccgacagcac gccgttgccc
     1261 gccgccatgc cttcgatcaa aagcgtgcgc ccgcactgga tcatgtcgag gcaggtggtg
     1321 gccagcagcg cctgatacag catgcccagc gccgcccgcg tgcgcggcgt gtccttgcgc
     1381 aggccgcgga tttcgccgcg cagccccgga tacgggccgc ccgcgtcgag gaaggagggc
     1441 agggccagcg ccgcctcgtg catgacctcg gccagggtcg cggcgtcgac gtccagcgcc
     1501 gaggcgcagc cgccttcggt gatcatctcg aacgcgcgtc cgcccatgaa gcgcgcgcac
     1561 ggcacggcgt tgccgaacac gtcgaccgcg cccatgcagt cgcgctcggc ctgaaggtgg
     1621 tcgagcgcgc cgccgggcgc catcgcgatg aaccacgtgc cggtggacaa aagggcgggc
     1681 aggtcgtcgt cctgctgcca gccgcccagc agcgccgcca gcgccgcgcc ggaatcgtgc
     1741 aggccgacgc agacgcgcgt cgtcgcgggc aggccggtca ggcgcgcgat gtcggccttg
     1801 agcggtccgg cggactccca cgccttgcgc agggccggga agcgccgcga ccagtcctga
     1861 tcgtgcgcca gcgaggaaaa gtcgttccgg cgcggcgccc acaggtcgct gtggcagccc
     1921 agcgaggtga tctccgcgtg cagcgcgccg ctcaggcgcc acgcccagta ttgcggatag
     1981 ggcagaatcc agcgcgccct ggcgaacagg gccgggtcgc ggtgctgatg ccaccagatc
     2041 tgcgcgccga ggttcagccc cttgggcagc ggcggcgaca gcgtctcgtc gaagccgggg
     2101 cggcggcgcg cataggcgcg cgcgaacgcg ggcgggatcg cgctttcata gtcctgaatc
     2161 ggctggagca ggttgaggcc gtcgtcgaga aacgcgcagg ccgcgccgtg cgccaccgga
     2221 atgatgcggt cgatgccgaa atcgctgaac gtcctgagtt gcgccagcag ccaggtgaac
     2281 agggcgtcgg tgtcgatggc ggtgtagagc ggggtcgcga aggtcggggt cggcgtgcgg
     2341 cgctgcgcca gcacctgacc cgacggcgtg accacgctga ccttggcgta ggtgcgtccg
     2401 gcgtcgaaga cgaggcaatt cggtccgttg cggctcctgc gttccctttt cacctttgcg
     2461 cttccctgct tcgcggtgag aattttgcgt ttcctgagcg attttgattg aaggcgacga
     2521 tttatggcgt tatcgtcgcg ttcgtaccgc ttattgtcat ttgtatgaca tctatagtcc
     2581 acctgcgccc gcagttcaat gcgtattaag ctttgacacg ggggtgtgaa tgatccgcca
     2641 acgatccgcc gtcggggcgg acgcgtaacc tcatgac
//
LOCUS       DASVLL010000519         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_195893,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000519 DASVLL010000000
VERSION     DASVLL010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195893"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF402_08585"
     CDS             <1..205
                     /locus_tag="VF402_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEX7800393.1"
                     /translation="KQAAARQLHKPVVTLIVKSTATGLPFYPASDYHQHFWQTHAAHY
                     DAYYQGCGRGPALHKLWGALADS"
     gene            complement(206..1852)
                     /locus_tag="VF402_08590"
     CDS             complement(206..1852)
                     /locus_tag="VF402_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7800394.1"
                     /translation="MTDDLKSQRDRYIAFSLAAADLLIEVDADFRIVRTIGATQALLN
                     AFAAEVVGRAAADIFTPGDRRFARRMLTRARAVGRIEPCALDLDQGDQPPLRVNLGAC
                     HLSGQDGHSFLSLTILSEAARLGSEGRDAASGLMEPGVYTAFAEQTLRRAGPGTPDDM
                     KLVRVRGLSRAMRDLPADRGRLLMAEIGAVLRAQSLNGVAAARLDEEAFSFLPSGAAA
                     SEQGLTQDIAAAARVAGLPDGALNATVINLQLSVGNLDPDSIARALSYVLSDFCRPDA
                     ARPVKDLESGLKAAMAETVGQFDSLRNLIDDGDYTLFYQPVVRLDDRSPHHYEALLRF
                     SDGRAPFDAVRMSEQLGLVQKLDLAVARKAVDMLNLRTDVKIAINLSGQSAQNDAFRE
                     ALRTLLLPHPDLSNRLLFELTESKAIEDMEAAANFLRWLRRTGYQVCLDDFGAGAATY
                     AYLRRFDVDYVKIDGPFLKEALDNTRQRALIRSIVSLTRDLKAETVAEMIEDETTLKL
                     CKELNVGYGQGFLFGKPKPQIDAPKALMVGKRKGFSESWG"
BASE COUNT          296 a          660 c          671 g          293 t
ORIGIN      
        1 gaaacaggcc gccgcccggc agttgcacaa gccggtcgtc accctgatcg tcaaatcgac
       61 cgccaccggc ctgcccttct acccggcatc cgactatcac cagcacttct ggcagaccca
      121 cgccgcgcat tacgacgcct attatcaggg ctgcggccgc ggcccggccc tgcacaaact
      181 ctggggcgcg ctggccgatt cgtgatcagc cccagctctc gctgaagccc ttgcgcttgc
      241 cgaccatcag ggccttgggc gcgtcgatct gcggcttcgg cttgccgaac aggaagccct
      301 ggccgtagcc gacgttcaac tccttgcaga gcttcagcgt cgtctcgtcc tcgatcatct
      361 cggcgacggt ttcggccttg aggtcgcgcg tcagcgacac gatggagcgg atcagcgcgc
      421 gctggcgcgt attgtcgagc gcctctttca ggaacggccc gtcgatcttg acgtaatcga
      481 cgtcgaagcg gcgcaggtag gcgtaggtcg ccgcgcccgc gccgaaatcg tcgaggcaga
      541 cctgatagcc ggtgcggcgc agccagcgca ggaagttggc cgccgcctcc atgtcctcga
      601 tcgccttcga ttcggtcagc tcgaacagca gccggttcga caggtccggg tgcggcagca
      661 acagggtgcg cagggcttcg cggaaggcgt cgttctgcgc cgactggccc gacaggttga
      721 tggcgatctt cacgtccgtg cgcaggttca gcatgtcgac cgccttgcgc gccacggcga
      781 ggtcgagctt ctgcacgagg ccgagctgct cgctcatgcg cacggcgtcg aacggcgcgc
      841 gcccgtcgga gaagcgcagc agcgcctcgt aatggtgcgg gctgcggtcg tcgaggcgca
      901 cgaccggctg atagaacagc gtgtagtcgc cgtcgtcgat caggttgcgc agcgaatcga
      961 actggccgac cgtctcggcc atcgccgcct tgaggccgct ttccagatcc ttcacgggcc
     1021 gggcggcgtc gggacggcag aagtcgctca gcacatagct gagcgcgcgc gcgatgctgt
     1081 ccgggtcgag attgccgacc gacagttgca ggttgatgac cgtggcgttg agcgcgccgt
     1141 cgggcaggcc cgcgacgcgc gcggcggcgg cgatgtcctg cgtcaggccc tgttccgacg
     1201 cggcggcgcc cgacggcagg aagctgaacg cctcctcgtc cagacgcgcg gcggcgacgc
     1261 cgttcagcga ctgggcgcgc agcacggcgc cgatctcggc catcagcagc cgacccctgt
     1321 cggcgggcag gtcgcgcatc gcccgcgaca ggccgcgcac gcgcaccagc ttcatgtcgt
     1381 ccggcgtccc cggcccggcg cggcgcaggg tctgttcggc gaacgccgta tagacgcccg
     1441 gctccatcag gcccgacgcg gcgtcgcgcc cctccgagcc gagacgcgcg gcctcggaca
     1501 gaatggtcag gctcagaaag ctgtggccgt cctgaccgga caggtgacag gcgccgagat
     1561 tgacgcgcag cggcggctga tcgccctgat cgagatcgag cgcgcacggc tcgatgcgcc
     1621 cgacggcgcg ggcgcgcgtc agcatgcggc gcgcgaagcg gcggtcgccc ggcgtgaaga
     1681 tgtcggcggc cgcgcgtccg acgacctcgg cggcgaaggc gttgagcagg gcctgggtcg
     1741 cgccgatggt gcggacgatg cggaaatccg cgtcgacctc gatcagcagg tcggcggcgg
     1801 ccagcgaaaa ggcgatataa cggtcgcgct ggcttttcag atcatcggtc aaaggacggc
     1861 gctccccggc ggccgcgctc tctttcgggg cggcggctga aaaggcagcc taggcccgac
//
LOCUS       DASVLL010000520         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_357020,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000520 DASVLL010000000
VERSION     DASVLL010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357020"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..2656)
                     /locus_tag="VF402_08595"
     CDS             complement(170..2656)
                     /locus_tag="VF402_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX7800395.1"
                     /translation="MRKTSERWFTVAGIVAMSLVALAGFAVAIYAAWLFHDMPDGSEL
                     ADFHPATSARVFAWDGTLIGQYGQERRIYVPYNRIPPRLREAFMAAEDRSFFQHNGVD
                     VGGVSRAMAKNVFNLLRGRRFEGGSTITQQVAKNLLLNNERTLGRKLKEVILARRLEK
                     NLSKEQILELYLNDIYLGYRAYGVGAAAYNYFGKSVDQLSLSEMAFLGALPKGPENYK
                     PSTHKAAAIARRNWVLGEMTKAGWVTQAEAQAAMKDDLVIQAKPLRAKYQDADYFVSE
                     VELRSKRLFDDKIYTDGYYIKTTLDPKLQTIARAALMNGLETYDRRHGWRGAWGSLDV
                     DDPNWIADAQDAEDKLPDNRKAPSERADWIVAMITKSGGAVRDVDGGEGAIKADDVAW
                     AKATRGLKAGDLVYVSKDSDGVYSLRQVPQVNGALVAVDPYSGRILALVGGYSYSLSK
                     FNRATQALRQPGSSIKPFVYAVALEHDFTPASIVDDSPVSMMGGDGKMWTPENYERDF
                     LGPQPIRRGIELSRNLMTIHLAQKVGIKPITQGIINFGVTDSMPNEMSMVLGAGEVNP
                     YRLVDAYAMFVNGGYKVKPHMISEVEDRNGHVVYKADERTCPSACTAAFDGLESPRLP
                     QIGAPVLDPITAYQMNSFLQGVTIRGTAAAARSLGFPIGGKTGTTNDFRSAWFVGFSA
                     DVVAGVFVGFDDNRSLGNHETGAVAALPIWMDFMKQAHAGKPARDFNKPKDAVYAYVN
                     GIQEAFKPGTEPKMQAAPVDGPRPYLDTWNGGDQGGAQNGGQDGAAPMTYVPSEEPPP
                     ASDKPGAPIVYVPQNDNAPRRSARNGKNQF"
BASE COUNT          471 a          957 c          887 g          491 t
ORIGIN      
        1 ggcgtaacgc gacatcccag tcaaagacgc ctcctgagca gaccgacgga ctgctcgata
       61 tcggcggcgg cggcctcaat atccaaacgc atggagaagt tcctgcaaaa aacgtgaggg
      121 gcgggtttat cctccccgcc cccggatgac aagtcagaac tgggacacgt cagaactggt
      181 ttttgccgtt gcgcgcgctg cgccgcggcg cgttgtcgtt ctgcggcaca tagacgatgg
      241 gcgcgcccgg cttatccgac gcgggcggcg gctcctcgga cggcacatag gtcatcggcg
      301 ccgcgccatc ctgcccgccg ttctgggcgc cgccctgatc gccgccgttc cacgtgtcca
      361 gatacggacg cggaccgtcc acgggcgcgg cttgcatctt gggctcggtg cccggcttga
      421 aggcttcctg aatgccgttg acataggcgt aaaccgcgtc cttcggcttg ttgaagtcgc
      481 gcgcgggctt gccggcgtgc gcctgtttca tgaagtccat ccagatcggc agcgccgcga
      541 ccgcgccggt ttcatgattg cccagcgagc ggttgtcgtc gaagccgacg aacacgcccg
      601 ccaccacgtc ggccgagaag ccgacgaacc acgccgagcg gaaatcgttg gtcgtgccgg
      661 tcttgccgcc gatcgggaag ccgaggctgc gcgccgccgc cgccgtgccg cgaatggtca
      721 cgccctgcaa aaagctgttc atctgatagg ccgtgatcgg gtccagcacc ggcgcgccga
      781 tctgcggcag gcgcgggctt tccagcccgt cgaacgccgc cgtgcaggcc gacggacagg
      841 tgcgctcgtc ggccttatag acgacatggc cgttgcggtc ctcgacctcc gagatcatgt
      901 gcggcttgac cttgtagccg ccgttgacga acatggcgta ggcgtcgacc aaccggtacg
      961 gattgacctc gcccgcgccc agcaccatcg acatctcgtt cggcatcgaa tcggtgacgc
     1021 cgaaattgat gatgccctgc gtgatcggct tgatgccgac cttctgcgcc agatggatgg
     1081 tcatcaggtt gcgcgacagc tcgatcccgc gccggatcgg ctgcggcccc aggaagtcgc
     1141 gctcatagtt ttccggcgtc cacatcttgc cgtcgccgcc catcatcgac accggcgagt
     1201 cgtcgacgat cgaggcgggc gtgaaatcgt gctccagcgc cacggcgtaa acgaacggct
     1261 tgatcgacga gccgggctgg cgcagcgcct gcgtggcgcg gttgaacttc gacagcgaat
     1321 aggaatagcc gccgaccagc gccaggatgc ggcccgaata gggatcgacc gcgaccaggg
     1381 cgccgttgac ctgcggcacc tgacgcaggc tgtaaacgcc gtcggaatcc ttcgacacat
     1441 agaccagatc gcccgccttc agcccgcgcg tcgccttggc ccaggcgacg tcgtccgcct
     1501 tgatcgcgcc ctcgccgccg tcgacgtcgc gcaccgcgcc gccggatttg gtgatcatgg
     1561 cgacgatcca gtccgcgcgc tcggacggcg ccttgcggtt gtccggcagc ttgtcctcgg
     1621 cgtcctgcgc gtccgcgatc cagttgggat cgtcgacgtc gaggctgccc cacgccccgc
     1681 gccagccgtg gcggcggtca taggtctcca gcccgttcat cagcgccgcc cgcgcgatcg
     1741 tctgcaattt cggatcgagc gtggtcttga tgtaatagcc gtcggtatag atcttgtcgt
     1801 cgaacaggcg ctttgaacgc agctcgacct cggagacgaa atagtcggcg tcctgatact
     1861 tggcgcgcag cggcttggcc tgaatgacca gatcgtcctt catcgcggcc tgcgcctcgg
     1921 cctgcgtgac ccagcccgcc ttggtcattt cgcccagcac ccagttgcgg cgcgcgatcg
     1981 ccgccgcctt gtgcgtggac ggcttgtagt tctccggccc cttgggcagg gcgcccagga
     2041 acgccatctc gctcagcgac aactgatcga ccgacttgcc gaaatagttg tacgccgccg
     2101 cgccgacgcc ataggcgcga tagccgagat agatgtcgtt caggtacagt tcgagaatct
     2161 gctccttgct gagattcttc tccagccgcc gcgccaggat cacttccttg agcttgcgcc
     2221 ccagcgtgcg ttcgttgttg agcagcaggt tcttggcgac ctgctgggtg atggtcgagc
     2281 cgccctcgaa acggcgcccg cgcaacaggt tgaagacgtt tttcgccatg gcgcgcgaca
     2341 cgccgccgac gtcgacgccg ttatgctgga agaagctgcg gtcctcggcg gccatgaagg
     2401 cctcgcgcag gcgcggcgga atgcggttgt acggcacgta gatgcggcgt tcctgaccgt
     2461 actggccgat cagggtgccg tcccaggcga agacgcgcgc cgaggtggcc gggtggaagt
     2521 cggccaactc cgaaccgtcc ggcatgtcgt gaaacagcca ggcggcatag atggccacgg
     2581 cgaaaccggc cagagccacc aggctcatgg ccacgatccc cgccacggtg aaccaacgct
     2641 cagacgtctt gcgcaaacgg cctccaactc aaccttcgcg gcggcctgtt cgcgcgcccg
     2701 ctgtcatccg gttccgacac ttaccaaacc gccgcccgca ggcaaagcga ttccggcgaa
     2761 aaagcgccgc cactccacgc acggacgcgg aaacgccgat gacccc
//
LOCUS       DASVLL010000521         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_34177, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000521 DASVLL010000000
VERSION     DASVLL010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34177"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VF402_08600"
     CDS             <1..677
                     /locus_tag="VF402_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800396.1"
                     /translation="KTVFNLLKSSRVDLMLLDVYVDGGNTLTHLPDLRALAPDAPIAV
                     MTAGGAGGQALQATLNMARRAQVDFVLPKPFGPEHLTPILDEVGRMRRAPELPRHILV
                     VDDCRVVRKLTTMALAEKGYRISEARTMEEAFERVDIAHVDVVLTDIFMPGMGGIEGI
                     GIIKSTWPAVAIVAMSAGAEHKLDHVQALAAAKYMGADALLPKPFTPEDMNLLVEAVL
                     EEKLAA"
     gene            904..1704
                     /locus_tag="VF402_08605"
     CDS             904..1704
                     /locus_tag="VF402_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800397.1"
                     /translation="MDENKEQAQRIASMLHQQKWTSVLSFDQQMAMRILKGGRFHLLL
                     FDAYAKGNNTLQMMDDIRADAQDAPLAIMSDGGHRSAALNAAMNAARVAGADFVIAKP
                     FNPEKLKNLLDDTNRYHRARAKERHVLVVEDDPDLRRDVVRVLKQVGYKVSWAANMED
                     VFFDHNLGLVDVVLTAVLIPGIGGIEGTAQIKKDHPHVKVIAMSQGVDETITAVHVLS
                     AAQAAGADALLAKPFHMPDLLRAMTGLIRAPDAPAENAKQAEIDALFD"
     gene            complement(1701..>2073)
                     /locus_tag="VF402_08610"
     CDS             complement(1701..>2073)
                     /locus_tag="VF402_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7800398.1"
                     /translation="HDVSVPLSRMADYIAEGDKAIQAFLPGARLVAFGHVGDGNVHFN
                     VIVPEGMDADAFNALRDEGAKVVHDLVHTYEGSISAEHGLGRMKTQEALLYKDPVAVA
                     AMRAVRLALDPRRIMNPHVLF"
BASE COUNT          384 a          648 c          699 g          342 t
ORIGIN      
        1 acaagacggt tttcaacctg ctgaaaagct cgcgcgtcga tctgatgctg ctcgacgtct
       61 atgtcgacgg cggcaacacc ctgacgcacc tgccggacct gcgcgcgctg gcgcccgacg
      121 cgcccatcgc ggtcatgacg gcgggcggcg cgggcgggca ggccttgcag gcaacgctga
      181 acatggcgcg gcgcgcgcag gtggatttcg tgctgcccaa gccgttcggg ccggaacacc
      241 tgacgccgat cctcgacgag gtcggccgga tgcgccgcgc gccggaactg cccaggcaca
      301 tcctcgtcgt cgacgactgc cgcgtggtgc gcaagctgac cacgatggcg ctggccgaaa
      361 aaggctatcg catcagcgag gcgcgcacga tggaggaggc gttcgagcgc gtcgacatcg
      421 cccatgtcga cgtcgtgctg accgacatct tcatgccggg catgggcggc atcgagggca
      481 tcggcatcat caagtccacc tggcccgccg tggcgatcgt ggccatgtcg gcgggcgccg
      541 agcacaagct ggaccacgtg caggcgctgg ccgccgcgaa atatatgggc gccgacgcct
      601 tgctgcccaa gccgttcacg ccggaggaca tgaaccttct ggtcgaggcg gtgctggaag
      661 aaaagctggc ggcgtaagaa aaccggcgtc ccgagggcgc gggtcagagt tgaggcggcg
      721 gtcccgccaa gtattgcagc gcaatattgt gacgatgacc tgcctcatag taccgtcctc
      781 aaaaggacgg ggcgtgctgt gcgtcattat tggcgagttg ttaagcactg cgacattatg
      841 attatttttt aaggcgtaaa agttggccgg ggagatcgta tgggtctggg cagcgtactt
      901 gtcttggacg aaaataaaga gcaggcgcaa cgcatcgcca gcatgctgca tcagcagaag
      961 tggacgtcgg ttctcagctt cgaccagcag atggccatgc ggattctcaa gggcggccgc
     1021 tttcatctgc tgctgttcga cgcctacgcc aagggcaata acaccttgca gatgatggac
     1081 gacatccgcg ccgacgctca ggacgcgccg ctggccatca tgtcggatgg cgggcaccgc
     1141 agcgccgcgc tcaacgcggc gatgaacgcc gcgcgcgtcg ccggcgccga cttcgtcatc
     1201 gccaagccgt tcaacccgga aaagctgaag aaccttttgg acgacaccaa ccgctatcac
     1261 cgcgcccgcg ccaaggagcg gcacgtgctg gtggtcgagg acgatcccga cctgcgccgc
     1321 gacgtggtgc gcgtgctgaa acaggtcggc tacaaggtgt cgtgggccgc caatatggag
     1381 gacgtgttct tcgaccacaa tctcggtctg gtggacgtgg tgctgacggc ggtgctgatc
     1441 cccggcatcg gcggcatcga aggcacggcg cagatcaaga aggaccaccc gcacgtcaag
     1501 gtcatcgcca tgtcgcaggg cgtggacgag acgatcacgg cggtgcacgt gctgtcggcg
     1561 gcgcaggcgg cgggcgcgga cgccctgctg gccaagccgt tccatatgcc cgacctgctg
     1621 cgcgccatga ccggcctgat ccgcgcgccg gacgctccgg cggagaacgc caagcaggcg
     1681 gagatcgacg ccctgttcga ttaaaacagg acgtgcgggt tcatgatccg cctggggtcg
     1741 agcgccagac gcaccgcgcg catggctgcg acggcgaccg ggtctttgta gagcagggcc
     1801 tcctgcgtct tcatgcggcc gaggccgtgc tcggcggaaa tcgagccttc gtatgtatgc
     1861 acgagatcgt ggacgacctt ggcgccttcg tcgcgcaggg cgttgaacgc atcggcgtcc
     1921 atgccttccg gcacgatgac gttgaagtgg acattgccgt cgccgacatg gccgaacgcc
     1981 accagccgcg cgccgggcag gaaggcctga atcgccttgt cgccttccgc gatatagtcg
     2041 gccatgcgcg acagcggcac cgagacgtcg tgc
//
LOCUS       DASVLL010000522         6050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_145249,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000522 DASVLL010000000
VERSION     DASVLL010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145249"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VF402_08615"
     CDS             complement(<1..624)
                     /locus_tag="VF402_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7800399.1"
                     /translation="MTTQPKPRIVILGAGLGGSIAAFEIRDALGSKAEIVVVNKGHTY
                     QFTPSNPWVAVGWRRREAIEVDLRPVFRKKGIELYAEGAARVDPVRKVVRLTGGADLS
                     YDYLVIATGPELAFDEIEGFGPHTGFTQSVCQADHAEAANAAFDALAAHPQPMVIGAA
                     QGASCFGPAYEFAMIVDTELRKRKARDRVPMTFVTSEPYIGHLGLDGV"
     gene            772..1104
                     /locus_tag="VF402_08620"
     CDS             772..1104
                     /locus_tag="VF402_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7800400.1"
                     /translation="MFGLFGPRTRTIDMTPAEVKAGLDAGELVLVDVREPDEHRAERI
                     QGARNMPLSRFDPKALPVDKTVVLHCAGGVRSARAVALCKQHDVDVRRHLAGGLAARK
                     AHGLPTRH"
     gene            1124..2149
                     /locus_tag="VF402_08625"
     CDS             1124..2149
                     /locus_tag="VF402_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7800401.1"
                     /translation="MTVRPRLAAVFLLSAAGLIAGLTACGEKTQSPAPAASNLPALTV
                     AAAEVDDLKPVAATLTSYRMAQATARLAGVLTSLDVREGDTVRAGQRIGFVQDSRIAP
                     QTSAYAAAAAAADAQAVQAQAQYNRVKTLYDKGIYARAALDQADAARKAAEADVRAAR
                     AQTAANAAYGDQGAIYAPDSGKVLSVAATTGAVVMMGQSVAVITSGAPVARIELPEGQ
                     SRALQVGGRVRLEADNRQAVGVIIRVYPSVTQGQVEADVTPAGFETLPVGAKITAWVS
                     LGRRRAVLLPRAYVTTRYGLDYVRLAQAGGGVMETVVETAPYDAQHVEIIGGLDAGDR
                     VIAYGAR"
     gene            2149..5397
                     /locus_tag="VF402_08630"
     CDS             2149..5397
                     /locus_tag="VF402_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7800402.1"
                     /translation="MESASQTGRLGLSGRLTKATLNSPLTPLALLVAILLGLIAVMSI
                     PREEEPQISVPMIDISVSDPGVRAADGVERVAKPLEAIVKSVPGVDHVYSFIDDDHVL
                     VTARFKVGTDPDNAAIRIHEKISANMDRLPAGVAEPLIQERGVNDVPALVLTLSPKPG
                     AGDRMTDQALYDLAVKLRAEVAKVDDVGLTFIVGGRPEEIRVEPDPALMRARGVSLDA
                     LTAAIKAADAAMPLGTLRDDNRSVLVGAGKTLQTAQDVGRITVPSIGGQPVLVQDVAT
                     VVEAPRQDQSQVFRYTEQKNGQWSQAPAVSLAVAKRQGANAVVLTRAILARVAALKGP
                     LIPAGVDVAVTRDYGESANEKANELLFHLGLATVSIVVLIGFAIGWREAGVTAVVIPT
                     TILLTLFASNLMGYTINRVSLFALIFSIGILVDDAIVMIENISRHWGMNDGRPRAQAV
                     VDAVAEVGNPTVIATLTVVAALLPMLFVSGLMGPYMAPIPVNASAAMVFSFFVAVILA
                     PWLMIRFARKALPGEAAVAHHDHEGFIGRIYRRVAAKVIADKKTAWRFLIGVGAATLL
                     ACAMFATKTVTVKLLPFDNKSELQVIADLPEGTTLETTQRVLAQAARIASRLKEVEAV
                     DSYAGTAAPFNFNGLVRHYYFRQYPEQGDLNVQLLPKGKRSRASHAIAIDLRNRLRAI
                     ALPQGGVLKVVEAPPGPPVMATLLAEIYGPDQATRRAVAEKVKGIFKSIPYIVDVDDS
                     YGAPQPRVDIAADRTRLEALNVSDGQVYAAVDTLMNGQVLGYSNRGQGREPLQIAVRL
                     PQSQRTLTSGILSMPVAVSQTAQGARLVALDEVADVTRDRSSTHLYRRDGRDVDMIMA
                     ELAGKYEAPIYGILDVDDRIRAADWGAAGTPDIRMHGQPQNEQHATVLWDGEWEITWV
                     TFRDMGAAFGVALLGIYALVVAQFKSFKLPLVILTPAPLTLIGIVIGHILFHAPFTAT
                     SMIGFIALAGIIVRNSILLVDFIQRHKLPGGGYDRPLKAVLLDAGATRFKPILLTALA
                     AMIGAAVILSDPIFQGLAISLLFGLLSSTLLTVLVIPAIYVALKSDDERVKS"
     gene            5481..5786
                     /locus_tag="VF402_08635"
     CDS             5481..5786
                     /locus_tag="VF402_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7800403.1"
                     /translation="MSAAPATTSELEDSAHSAARLLKLLASEQRLLILCKLIDRECSV
                     GELSEHIHLTQSATSQHLARMRAEGLVDTRRDAQTIYYRLKDPAAIRIIRTLSEIYC"
     gene            complement(5828..5903)
                     /locus_tag="VF402_08640"
     tRNA            complement(5828..5903)
                     /locus_tag="VF402_08640"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5868..5870),aa:Ala,seq:ggc)
BASE COUNT         1002 a         2079 c         1986 g          983 t
ORIGIN      
        1 gacgccgtcg aggccgagat ggccgatata gggctcggac gtcacgaagg tcatcggcac
       61 gcggtcgcgc gccttgcgtt tcctcagctc ggtatcgacg atcatggcga attcgtaggc
      121 cgggccgaag caggacgcgc cctgcgccgc gccgatcacc atcggctgcg gatgggcggc
      181 cagggcgtcg aacgccgcgt tcgccgcctc ggcgtgatcg gcctggcaga ccgactgggt
      241 aaagccggtg tgcggcccga aaccctcgat ctcgtcgaag gccagttccg gtcccgtggc
      301 gatgaccaga tagtcgtagg acaggtccgc gccgccggtc agacgcacga ccttgcggac
      361 cggatcgacg cgggccgcgc cttcggcata gagctcgatc ccctttttcc gaaacacggg
      421 gcgcagatcg acctcgatgg cttcgcgcct gcgccagccg acggccaccc acggattgga
      481 aggcgtgaac tgataggtgt ggcccttgtt gaccacgacg atctcggcct tcgagccgag
      541 ggcgtccctg atttcaaaag cggcgatcga gccgcccaga cccgcgccca gaatgacgat
      601 gcgcggcttt ggctgagtgg tcattacggt cctcccggtt ttttcgcccg aacctcgcgc
      661 cgcaaattca gcccgaaatt caggttgacc taatatgcat gttatcttat attagaaata
      721 tagaatatgc aaatattctt ttcatgcaac ccgcaccggc aggaggccgc catgttcgga
      781 cttttcggac ccaggaccag aaccatcgat atgacgcccg ccgaggtgaa ggcggggctg
      841 gacgcgggcg aactcgtcct cgtcgacgtg cgcgaaccgg acgagcatcg cgccgagcgc
      901 atccagggcg cgcggaacat gccgttgtcg cgcttcgatc cgaaggcctt gccggtggat
      961 aaaaccgttg tgctgcattg cgcgggcggc gtgcgttcgg cgcgcgccgt cgcgctgtgc
     1021 aaacagcacg atgtcgacgt ccggcgtcat ctggcgggcg gcctcgccgc gcggaaggcc
     1081 cacggcctgc cgacccggca ttgagttgtc gagagacccc gccatgaccg tccgtccccg
     1141 tctcgccgcg gttttccttt tgagcgccgc cggcctgatc gccggtctga cggcctgcgg
     1201 cgaaaagacg caaagccccg cccccgccgc gtcgaacctg cccgccctca ccgtcgccgc
     1261 cgccgaggtc gacgatctca agccggtggc cgcgaccctg accagctaca ggatggcgca
     1321 ggcgacggcg cggctggcgg gcgtgctgac ctcgctggac gtgcgggagg gcgacacggt
     1381 ccgggccggg caacggatcg gcttcgtgca ggattcgcgc atcgcgcccc agacctcggc
     1441 ctatgcggcg gcggcggcgg cggcggacgc ccaggccgtg caggcgcagg cgcaatataa
     1501 ccgcgtcaag acattgtacg acaagggcat ctacgccagg gccgcgctgg atcaggccga
     1561 cgccgcccgc aaggccgccg aggccgacgt cagggcggcg cgcgcccaga cggcggccaa
     1621 cgccgcctat ggcgatcagg gcgcgatcta cgcccccgat tccggcaagg tgctgagcgt
     1681 cgcggcgacc acgggcgcgg tggtgatgat gggccagtcc gtcgccgtca tcacctccgg
     1741 cgcgccggtg gcgcgcatcg aactgcccga aggccagagc cgggccttgc aggtcggcgg
     1801 gcgcgtgcgt ctggaggccg ataaccggca ggcggtcggc gtgatcatcc gcgtctatcc
     1861 gtcggtgacg cagggtcagg tcgaggccga cgtcacgccc gccgggttcg agaccctgcc
     1921 ggtcggcgcc aagatcacgg cctgggtgtc gctggggcgg cggcgggccg tgctgctgcc
     1981 gcgcgcctat gtgacgacgc gctacggcct cgactatgtc aggctggcgc aagcgggcgg
     2041 cggcgtgatg gaaaccgtgg tcgagaccgc gccctacgac gcgcagcatg tggaaatcat
     2101 cggcggcctc gatgcgggcg accgcgttat cgcttacgga gcgcgctgat ggagtccgcg
     2161 tcgcaaaccg gccgtctggg cctgtcgggc cgtctgacta aggccacgct caactcgccg
     2221 ctgacgccgc tggccctgct ggtggcgatc ctgctgggcc tgatcgccgt catgtcgatc
     2281 ccgcgcgagg aggagccgca gatcagcgtg ccgatgatcg acatctcggt gtccgatccc
     2341 ggcgtgcgcg ccgccgacgg cgtcgaacgg gtcgccaagc cgctggaggc catcgtcaaa
     2401 agcgtgcccg gcgtcgatca cgtttacagc ttcatcgacg acgatcatgt gctggtgacg
     2461 gcgcgcttca aggtcggaac ggaccccgac aacgccgcga tccgcatcca tgaaaagatt
     2521 tccgccaata tggaccgcct gcccgccggg gtggccgagc cgctgattca ggagcgcggc
     2581 gtcaacgacg tgccggcgct ggtgctgacc ctgtcgccga aaccgggcgc gggcgaccgc
     2641 atgaccgatc aggcgctcta cgatctggcg gtcaagctgc gcgccgaggt cgccaaggtc
     2701 gacgatgtcg gcctgacctt catcgtcggc ggccgcccgg aggaaatccg cgtcgagccc
     2761 gatccggccc tgatgcgcgc ccgcggcgtc tcgctcgacg ccctgaccgc cgcgatcaag
     2821 gccgccgacg ccgccatgcc gctcggaacc ctgcgcgacg acaaccggtc cgtgctggtc
     2881 ggcgcgggca aaacgctgca aaccgcgcag gatgtcggcc gcatcactgt gccgtcgatc
     2941 ggcggccagc ccgtgctggt gcaggacgtc gccacggtgg tcgaggcgcc gcgtcaggac
     3001 cagagccagg tcttccgcta caccgaacag aagaacggac aatggtcgca ggcgcccgcc
     3061 gtatcgctgg ccgtcgccaa gcgtcagggc gccaacgccg tcgtcctcac ccgcgcgatc
     3121 ctcgcccgcg tcgcggcgct gaaagggccg ctgatcccgg cgggcgtcga tgtcgcggtc
     3181 acgcgcgact atggcgagag cgccaatgaa aaggccaatg agctgctgtt ccatctgggg
     3241 ctggcgaccg tctccatcgt cgtcctgatc ggcttcgcca tcggctggcg cgaggcgggc
     3301 gtcacggcgg tggtcatccc gaccaccatc ctgctgaccc tgttcgcctc caacctgatg
     3361 ggctacacca tcaaccgcgt cagcctgttc gcgctgatct tctccatcgg gattctggtc
     3421 gacgacgcca tcgtcatgat cgagaacatc tcgcgccact ggggcatgaa tgacggacgc
     3481 ccgcgcgcgc aggccgtcgt cgacgccgtg gccgaggtcg gcaacccgac cgtgatcgcc
     3541 accctgaccg tggtggcggc gctgctgccc atgctgttcg tgtccggcct gatggggccg
     3601 tacatggcgc cgatcccggt caacgcctcg gcggcgatgg tcttttcctt cttcgtcgcc
     3661 gtcatcctgg cgccgtggct gatgatccgc ttcgcgcgca aggccctgcc cggcgaggcg
     3721 gccgttgcgc atcacgatca tgagggcttt atcggccgga tctaccgccg cgtcgccgcg
     3781 aaggtgatcg ccgacaaaaa gaccgcctgg cgcttcctga tcggggtcgg cgccgcgacc
     3841 ctgctcgcct gcgccatgtt cgccaccaag acggtgacgg tgaagctgct gcccttcgac
     3901 aacaagtcgg aattgcaggt catcgccgac ctgccggaag ggacgacgct ggagaccacg
     3961 cagcgcgtgc tggcgcaggc cgcgcgcatc gcctcgcgcc tgaaggaagt cgaggcggtc
     4021 gattcctacg ccgggaccgc cgcgccgttc aatttcaacg gtctggtgcg ccactactac
     4081 ttccggcaat atcccgaaca gggcgacctg aacgtgcaac tcctgcccaa gggcaagcgc
     4141 tcgcgcgcca gccacgccat cgccatcgac ctgcgcaacc gtctcagggc catcgcgctg
     4201 ccgcagggcg gcgtgctgaa ggtggtcgag gccccgccgg gaccgccggt catggccacc
     4261 ctgctggccg aaatctacgg cccggatcag gccacgcgcc gcgccgtggc ggagaaggtc
     4321 aagggcatct tcaagtcgat cccctatatc gtcgatgtcg acgattccta cggcgcgccg
     4381 cagccgcgcg tcgatatcgc cgccgaccgc acccggctgg aggcgctcaa cgtctccgac
     4441 ggtcaggtct atgccgccgt cgatacgctg atgaacggtc aggtgctggg ctattccaac
     4501 cggggtcagg ggcgcgagcc gttgcagatc gccgtgcgcc tgccgcaatc gcagcgcacc
     4561 ctgacctcgg gaatcctgtc catgccggtg gccgtttcgc agaccgcgca gggcgcgcgc
     4621 ctcgtcgcgc tcgacgaagt ggccgacgtg acgcgcgacc ggtcctcgac ccacctctat
     4681 cgccgcgacg ggcgcgacgt cgacatgatc atggccgaac tggcgggcaa gtacgaggcg
     4741 ccgatctacg gcatactcga cgtggacgac aggatcaggg ccgccgactg gggcgcggcc
     4801 ggaacccccg acatccgcat gcacggccag ccgcagaacg aacagcacgc caccgtgctg
     4861 tgggacggcg aatgggagat cacctgggtg accttccgcg acatgggcgc cgccttcggc
     4921 gtggccctgc tcggcatcta cgccctcgtc gtggcgcagt tcaaatcgtt caaactgccg
     4981 ctggtcatcc tgacgccggc gccgctgacc ctgatcggca tcgtgatcgg ccacatcctg
     5041 ttccacgccc cgttcacggc gacctcgatg atcggcttca tcgccctggc cgggatcatc
     5101 gtgcgcaact cgatcctgct ggtcgatttc atccagcgcc acaaattgcc gggcggcggc
     5161 tatgaccggc ccctaaaggc ggtgctgctc gacgcgggcg ccacgcgctt caagccgatc
     5221 ctgctgacgg cgctggcggc gatgatcggc gcggcggtga tcctgagcga tccgatcttt
     5281 cagggtctgg ccatctcgct gctgttcggt ctcctgtcct cgaccctgct gaccgtgctg
     5341 gtcattccgg ccatctatgt cgcgctgaaa tccgatgatg aacgggtgaa atcatgaccg
     5401 gcccgcgcag aacggttgac aaggcggccg ggactgacaa agtccggaac ccggtccatc
     5461 ccagccatga gtgtccgcat ttgtccgccg cgcccgccac aacctcggaa ctggaagaca
     5521 gcgcccattc ggcggcccgt ctgctgaagc tgctggccag cgagcaacgc ctgctgatcc
     5581 tgtgcaagct gatcgaccgg gaatgcagcg tgggcgaatt gtccgagcat atccatctga
     5641 cccagtcggc gacctcgcag catctggcca ggatgcgcgc cgaggggctg gtcgatacgc
     5701 gccgcgacgc ccagaccatc tattaccgcc tgaaagaccc ggcggcgatc cgcatcatca
     5761 gaaccctgtc ggaaatctac tgctgagcgg ccattaaaaa accccgctgc gaggcggggt
     5821 tttttaatgg tggagctaag cggagtcgaa ccgctgacct cttgaatgcc attcaagcgc
     5881 tctaccaact gagctatagc cccatcctta aacctcacgc caaaaagtgg aagccgcttt
     5941 ctggaaacat atgaggcgtc gtaaattcgg agcgcaccgc gctccgggat ggtcccggaa
     6001 ggcgtggccc gccgaggccg cgacttctat ttcaaggctt ggccgcgcgc
//
LOCUS       DASVLL010000523         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_145288,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000523 DASVLL010000000
VERSION     DASVLL010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145288"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF402_08645"
     CDS             complement(<1..839)
                     /locus_tag="VF402_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7800404.1"
                     /translation="MNAAASGSFKIGGDLEVHRLGYGAMRITGPGVWGPPHDRAEAIR
                     TLRRLPELGVNFIDTADSYGPDVSEHLIRAALHPYDGLIVATKGGLTRKGPNIWIPNG
                     DPDYLIGQARKSREQLGVERIDLWQLHRIDPKVPRDEQFAAVRRLLDDGVIRHAGLSE
                     VSVEDIEAARKVFPVATVQNKYNLVERQSEAVLDYCEANAIGFIPWYPLAAGELARPG
                     GAVDTVARRYGATAGQIALAWVLKRSPVMLPIPGTGKVRHLEENVAAADIVLSDADFA
                     ALD"
     gene            complement(899..>1513)
                     /gene="prfC"
                     /locus_tag="VF402_08650"
     CDS             complement(899..>1513)
                     /gene="prfC"
                     /locus_tag="VF402_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516496.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX7800405.1"
                     /translation="TRGMKLKVQNTGKQISVNAPIMFFASDRELAENAYGGDVIGIPN
                     HGVLRVGDSLSESGAVRFAGLPNFAPEILQRVRVKDPLKAKHLKKALESLAEEGVSQL
                     FRPVIGSDFIVGAVGQLQFEVMADRLANEYGLECQFEPSPYAEARWLSGDAAKIEDFA
                     GKYRAQMGTDIDEAPVFLGKSGWEINYVAERFPDVRFLRAKERA"
BASE COUNT          238 a          518 c          488 g          269 t
ORIGIN      
        1 ttgtccagag cggcgaaatc ggcgtcgctg aggacgatgt ccgccgccgc gacgttttcc
       61 tccagatgcc ggaccttgcc ggtgccgggg atcggcagca tgacgggcga gcgcttcagc
      121 acccaggcca gcgcgatctg gcccgccgtg gcgccgtagc ggcgcgcgac cgtatcgacc
      181 gcgccgccgg gccgcgccag ttcgcccgcc gccagcggat accacgggat gaagccgatc
      241 gcgttcgctt cgcaatagtc cagcacggcc tcgctctggc gctcgaccag attgtacttg
      301 ttctgcaccg tggcgacggg aaagaccttg cgcgccgcct cgatgtcctc gaccgagact
      361 tccgacaggc ccgcatgacg gatcacgccg tcgtccagca ggcgccggac cgccgcgaac
      421 tgttcgtcgc gcggaacctt ggggtcgatg cggtgcagtt gccacaggtc gatgcgctcg
      481 acgccgagct gttcgcggct cttgcgcgcc tgcccgatca gatagtcggg atcgccgttc
      541 ggaatccaga tgttcggccc cttgcgcgtc agcccgccct tggtggcgac gatgaggccg
      601 tcatagggat gcagcgccgc gcggatcagg tgttcggaca cgtccgggcc ataggaatcg
      661 gcggtgtcga tgaagttcac gcccagttcg ggcaggcgcc gcaaggtgcg gatcgcctcg
      721 gcgcggtcgt gcggcggccc ccagacgccc ggcccggtga tgcgcatcgc gccgtagccc
      781 agacgatgca cctcaagatc gccgccgatt ttgaagcttc ccgatgccgc tgcgttcatg
      841 acctgctcct tttgccgcat gcgccctgag atgggcgcgg gacggcgcgg atgcaagctc
      901 aggcgcgctc tttcgcgcgc aggaatctca cgtccgggaa gcgctcggcg acatagttga
      961 tctcccagcc ggacttgccg aggaagaccg gcgcttcgtc gatgtcggtt cccatctggg
     1021 cgcggtactt gcccgcgaag tcctcgatct tcgccgcgtc gccgctcagc cagcgcgctt
     1081 ccgcataggg ggaaggctcg aactggcatt ccagcccgta ttcgttggcc aggcggtcgg
     1141 ccatgacctc gaactgcaac tgcccgacgg cgccgacgat gaagtccgaa ccgatcaccg
     1201 gacggaacag ttgcgacacc ccctcctcgg ccagagattc cagcgccttt ttcaggtgct
     1261 tggccttgag cgggtctttc acgcgcaccc gttgcaggat ttccggcgcg aaattgggca
     1321 ggcccgcgaa acgcaccgcg ccgctttccg acaaagagtc gccgactcgc agcaccccgt
     1381 ggttcggaat gccgatcacg tcgccgccat aggcgttttc cgccagctcg cggtccgagg
     1441 cgaagaacat gatcggcgcg ttgaccgaaa tctgcttacc ggtgttctga accttcagct
     1501 tcatgccgcg cgt
//
LOCUS       DASVLL010000524         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_145321,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000524 DASVLL010000000
VERSION     DASVLL010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145321"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VF402_08655"
     CDS             complement(<1..1088)
                     /locus_tag="VF402_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7800406.1"
                     /translation="MASEIHIHNGPLIIGAGLAGLSAALACAPHKVLVLSPVPLGQAA
                     SSAWAQGGMAAALSAGDSPAAHALDTVNAGAGLVDAEAARALTEAGPGTVRRLNALGA
                     PFDKTPDGGFVLNREAAHGLARVARVGGDLAGRKILEAVVAAAQKADHIEIWDDAHVL
                     GLIRSDARTVTGARVRRGDRLIDVYAPAVILATGGSGGLYAVTTNPSALRGEGMAMAA
                     LAGAQIADPEFVQFHPTALKVGRDPAPLATEALRGEGAVLIDARGERFAFDDHPDGEL
                     APRDIVARAVHKANLHRGGAFLDATQAIGAHFDKEFPTVFAACMAEGIDPRVQPMPVA
                     PAAHYHMGGVRTDLDGRTSLKGLYAVGE"
     gene            complement(1221..2347)
                     /gene="nadA"
                     /locus_tag="VF402_08660"
                     /pseudo
     CDS             complement(1221..2347)
                     /gene="nadA"
                     /locus_tag="VF402_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920750.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
BASE COUNT          373 a          835 c          876 g          417 t
ORIGIN      
        1 cattcgccga ccgcataaag gcccttcagc gatgtgcggc cgtcaaggtc ggtgcggacg
       61 ccgcccatat ggtaatgcgc ggcgggcgcg accggcatgg gctggacgcg cgggtcgatc
      121 ccttcggcca tgcaggcggc gaagacggtc gggaattcct tatcgaagtg ggcgccgatg
      181 gcttgcgtgg cgtcgaggaa ggcgccgccg cgatgcagat tcgccttatg caccgcgcgc
      241 gccacgatgt cgcgcggcgc cagctcgccg tccgggtggt cgtcgaaggc gaagcgctcg
      301 cccctggcgt cgatcagcac ggcgccttcg ccgcgcagcg cctcggtggc cagcggcgcc
      361 ggatcgcggc cgaccttgag cgcggtcgga tggaactgca cgaactccgg gtcggcgatc
      421 tgcgccccgg ccagcgccgc catcgccatg ccctcgccgc gcaaggcgga cggattggtg
      481 gtgacggcgt aaagcccgcc cgagccgccg gtggccagaa tgacggcggg cgcatagacg
      541 tcgatcagcc tgtccccgcg ccggacccgc gcgccggtca cggtccgtgc gtccgatctg
      601 atcaggccga gcacatgggc gtcgtcccag atttcgatat ggtcggcctt ttgcgccgcc
      661 gcgaccacgg cttcgagtat cttcctgccc gccaggtcgc cgccgacgcg cgccacgcgg
      721 gccaggccgt gcgcggcttc gcgattgagc acgaaaccgc cgtccggcgt cttgtcgaac
      781 ggcgcgccga gggcgttgag gcggcgcacc gtgccgggac cggcctcggt cagggcgcgc
      841 gcggcctcgg cgtcgacgag gcccgctccg gcgttgacgg tgtcgagcgc gtgcgcggcg
      901 ggggaatccc cggcggacag cgccgccgcc atgccgccct gcgcccaggc ggacgaggcg
      961 gcctgaccca gcggaaccgg cgacaggacg agaaccttgt gcggcgcgca ggcgagggcg
     1021 gcggacagcc ccgccagccc cgcgccgata atcagcgggc cgttatggat gtggatttcg
     1081 ctggccatgt tggcctcctg aagcggtttg ccccctccgt cggcttcgcc gccgcctccc
     1141 ccgctgtcgc agggggggaa gagacgacct gcctccgttt acgggaggga gcagcgatgc
     1201 ggcggtgggg gaagggagcc ttagctgatc atctcgacgt cgacatgatg ccgcgccttg
     1261 accatgtcgt agcgggcggg cgtcgtcagc ggcggcatgt cgatcatgcg ctgcacggcg
     1321 gcggcggcgc gcgggatcag ggccggatcg acgtcgacct catgccggcg caggcgcaag
     1381 gagtcgtaaa tgccttgcag ggtgatgcgc ttcatgtgcg ggcacaggtt gcaggggcgc
     1441 aggaactcca cgcccggcgc gtcggaggcg acgttgtcgg ccatcgaaca ttcggtgatc
     1501 agcaccacct gcgcgggctt gttcctcgtc acatagtcgc tcatcgccgt ggtcgagccg
     1561 gtgaagtccg aggccgcgat cacgtcgggc ggacattccg ggtgggccag cacgaccgcg
     1621 ttcggatggg ctttgcgcac ctcggcgatg tcggcggcgt cgaagcgctc atgcacctcg
     1681 caggcgcccg cccaggcgat gatcttcacg ccggtctggc gcgcgacgtt cctggccagg
     1741 aactgatcgg gcagcatgat gaccgtatcg acgccccatt cccgcgcgat atgctcgacc
     1801 acctgcaccg cattggccga ggtgcagcag atgtcggtct cggccttcac ctccgccgtg
     1861 gtgttgacat aggtgacgac cggcaggccg ggatagcgct gcttgatcag ccgcacctgc
     1921 tcgccggtga tgctggaggc cagcgagcag cccgcgcgca tgtccgggat cagcacggtc
     1981 ttttccggcg ccagaatctt cgaggtctcg gccatgaaat gcacgccgcc ctgcacgatg
     2041 atcgcggcgt cggacttggc cgcctcgcgc gccagcccca gcgagtcgcc gacatagtcg
     2101 ccgacgccgt ggaagatgtc cggcgtcatg tagttgtgcg ccagcacgac cgcgttcatc
     2161 tcgcgcttca gccggttgat agcggcgatc agcggcgcct gcagccgcca ttccagatcc
     2221 gagaccttgc ctttcaggcg gtcatggatc gcggcggttt cggcctcgat ctgcggggtg
     2281 aattccagcg gcagggcggt gtccagcatg agcctatcct caaaccactt atgctcaaat
     2341 tcagcataag gtggctataa catagtcttt gccgcgcgcg atgcaatacg cttttacgga
     2401 cggggcttca cgttttatga cagagccgtg atgggcgtcc tgtgaaggtt tacctgtggc
     2461 gcgtcgccgc tccgcgccga atccaccttg aatccttatg g
//
LOCUS       DASVLL010000525         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_196507,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000525 DASVLL010000000
VERSION     DASVLL010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196507"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..900)
                     /gene="paaC"
                     /locus_tag="VF402_08665"
     CDS             complement(148..900)
                     /gene="paaC"
                     /locus_tag="VF402_08665"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX7800407.1"
                     /translation="MSTAYFEYTLRLGDDALLLGQRLSAWCGHAPSLEVDLGLSSLAL
                     DLVGQATFWLEQAAKHDPQGRSADALAFHREARDFHNCLMVEQPNGDFAQTLARQFLY
                     STYQALLMEALAASADREIADIAARCAPDIAYHVRFAADWMVRLGDGDCLSHRRLVKS
                     LGYMWHFVDELFVMDEIDHALLRAGIAADKAALRPEFDARVDAVLREAGLPAPEGRRA
                     VINGRKGHDGAHIAHMLHEMNIPPQEGHDLPW"
     gene            complement(1262..2329)
                     /locus_tag="VF402_08670"
     CDS             complement(1262..2329)
                     /locus_tag="VF402_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX7800408.1"
                     /translation="MIVRAARLAAALIAPILIAPAFIACGAQAAPKPAIIAYLPTFRG
                     DMAAHLDAVDLSRLTQIDIAFVNPAPDGAVVRADALACEVRPQVGGATAATLAELRTV
                     VSRAHAAGVKVFASLGGGVIPACAGDWATLLSPAMRPLVEANLLQFARDFDLDGLDID
                     LEWRVLSAVDAAGDYTPFARDLSRALHRDGKRLSCATASQPGGMIPAAAIPYFDTIGV
                     MSYDGVGPDWGTPGEEHATLDMARRDLAVWRARGARKDRLVLGVPFYGHGFGAYKGGY
                     DYKDILAAHGPEAAESDVVGRLCAGCDYITYNGRPTIRLKAELARTGAAGVMIWDITG
                     DAAGADSLLGALYESLNADAK"
     gene            2639..>3507
                     /locus_tag="VF402_08675"
     CDS             2639..>3507
                     /locus_tag="VF402_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7800409.1"
                     /translation="MSRPNTRILPVLAVLCSILSVQAGASFSKHLFPALGAEGVTALR
                     QLYSALLLLLVFRPWTGGPARKDWGVVLLYGALLGIMNLTFYLAIARLPLGIGVALEF
                     LGPLTVAIVSSRQARDFVWVACAVAGLALLIPWNSHTGALDPVVVAWALVAALCWGLY
                     IIVGQKVSDRAHGGKAVALGMTFSLVLTLPVGMIHAGAALWAPAILPWAVAVAVLSSA
                     IPYSLEMMALRHIPAKTFGLMMSLEPAAGATLGFLIVGERLSLTQVAAIALVIVASAG
                     SSLTARRPVDESLP"
BASE COUNT          545 a         1151 c         1204 g          607 t
ORIGIN      
        1 cattttgccg caacgagaac acggtattcg gcatctgccg caatttgatg gaacccgagc
       61 agagcaacta agcattccag cccgctaacg gtaagccccc acattgtcgt tacgcaaccg
      121 ttattcacgt aaagagacgg gatggcgtca ccacggcagg tcgtggcctt cctgcggcgg
      181 gatgttcatc tcgtgcagca tatgggcgat gtgggcgccg tcgtggccct tgcggccgtt
      241 gatgacggcg cgccggcctt ccggcgcggg caggcccgct tcgcgcagca cggcgtcgac
      301 gcgcgcgtcg aattcggggc gcagcgcggc cttgtcggcg gcgatcccgg cgcgcagcag
      361 cgcatggtcg atctcgtcca tcacgaacag ttcgtcgacg aagtgccaca tatagccgag
      421 gctcttgacc agcctccggt ggctcaggca gtcgccgtcg cccagccgca ccatccagtc
      481 ggcggcgaac cggacatgat aggcgatgtc gggcgcgcag cgcgcggcga tgtcggcgat
      541 ctcccggtcg gcggaggcgg ccagcgcctc catcagcagc gcctgatagg tcgaatagag
      601 gaattgccgc gccagggtct gggcgaaatc gccgttcggc tgctcgacca tcaggcagtt
      661 gtggaagtcg cgcgcctcgc gatgaaaagc cagcgcgtcg gcgctgcggc cctgcggatc
      721 gtgcttcgcc gcctgctcca gccagaaggt cgcctgaccg accagatcga gcgccagact
      781 ggacaggccc agatcgactt cgaggctggg ggcgtggccg caccacgcgc tcaggcgctg
      841 gccgagaagc agggcgtcgt cgccgagacg aagcgtgtat tcaaaatagg cggtggacat
      901 gacgtttcct ttggcggcgc ggttcgcgct ttttgtctaa gaatggaggt ttgctctgac
      961 ggttttatgc tgcgcgtcag ggcgcagcat tacggtcgtg acgatgccgc cgggcggacg
     1021 ggccgcgacc ggcgcgcaag accgcgcaca ggcggcatcg ggtcacgcaa aggacagagg
     1081 cggcgggcat taacaaattc tgccgtgaat tcttatcgtt acccggagtc taaccaaaag
     1141 gcggcgccgg gtcaatttca aaactgacgt ttatgtcaat tttatatttc ttccgacacg
     1201 gcgcaatggc gtcctgattt cgggcgcgat cgtcggaaat ttttgctgcg ccatgctgcg
     1261 cttatttcgc atccgcgttc agcgactcgt acagcgcgcc cagcaggctg tccgcgcccg
     1321 ccgcgtcgcc ggtgatgtcc cagatcatca cgccggcggc gcccgtccgc gccagttccg
     1381 ccttgagccg gatcgtcgga cggccgttat aggtgatgta gtcgcagccc gcgcaaaggc
     1441 ggccgaccac atcgctttcg gcggcttcgg ggccgtgcgc ggccagaatg tccttgtaat
     1501 cgtagccgcc cttataggcg ccgaagccgt ggccgtagaa cggaacgccc agcacgagcc
     1561 ggtccttgcg cgccccccgc gcgcgccaga cggccaggtc gcggcgcgcc atgtcgagcg
     1621 tggcgtgttc ctcgcccggc gtcccccagt ccgggccgac gccgtcatag gacatgacgc
     1681 cgatcgtgtc gaagtacggg atcgccgccg ccgggatcat gccgcccggc tgcgaggcgg
     1741 tcgcgcagct caggcgcttg ccgtccctgt gcagggcgcg gctcagatcg cgcgcgaacg
     1801 gcgtatagtc cccggcggcg tccacggcgc tcagcacgcg ccattccaga tcgatgtcga
     1861 ggccgtcgag gtcgaaatcg cgcgcgaatt gcagcagatt ggcctcgacc agcggccgca
     1921 tggcgggcga cagcaaggtc gcccagtcgc ccgcgcaggc cgggatgacg ccgccgccca
     1981 gcgaggcgaa caccttgacg cccgccgcat gggcgcggct gacgaccgtg cgcaactccg
     2041 ccagcgtcgc cgccgtcgcg cccccgacct gcggccgcac ctcgcaggcc agggcgtctg
     2101 cgcgcacgac ggcgccgtcg ggcgccgggt tgacgaaggc gatgtcgatc tgggtcagac
     2161 ggctcaggtc gacggcgtcg agatgggcgg ccatatcgcc cctgaaggtt ggcaggtagg
     2221 cgatgatcgc gggcttcggc gccgcctgcg ccccacaggc gatgaaggcg ggcgcgataa
     2281 ggataggcgc gatgagagcg gcggccagcc gcgcggcgcg gacgatcatg agcgtttccc
     2341 ctgttcggac cccagcctga cagcgcgcgc ggcggctggc aataagacca atccgatttt
     2401 gcgtcgcgcc tgtttccgcc gcggggggtt gagacatgtc gttgcaattg ctatctgtgc
     2461 ggcgaccccg gacgagggac gtcgtacccg gtcaagctag agcgctttcc gaaaagtgtg
     2521 acgcactttt cggattaaaa agcgtgtaaa agcataaagt tagagcgccg atctgatgca
     2581 atcagatcgg aaaacgctct aagacgacgt cgcaacctta tcgccgccgg agtccgccat
     2641 gtcccgtccc aacacacgca tcctgccggt tctggccgtg ctgtgctcga tcctgtccgt
     2701 gcaggcgggc gcctcgttca gcaagcacct gttccccgcg ctgggcgccg agggggtgac
     2761 cgccttgcgc cagctctatt ccgccctgtt gctgctgctg gtgttccgcc cgtggaccgg
     2821 cggtccggcg cgcaaggact ggggcgtggt gctgctgtac ggcgcgcttc tgggcatcat
     2881 gaacctgacc ttctacctcg ccatcgcgcg gctgccgctg ggcatcggcg tggcgctgga
     2941 gttcctgggg ccgctcaccg tggccatcgt ctcgtcgcgg caggcgcgcg atttcgtctg
     3001 ggtggcgtgc gccgtggcgg ggctggccct gctgatcccg tggaacagcc acaccggcgc
     3061 gctcgatccg gtcgtcgtgg cgtgggcgct ggtcgcggcc ctgtgctggg ggttgtacat
     3121 catcgtcggc cagaaggtgt ccgaccgcgc gcacggcggc aaagcggtgg cgctgggcat
     3181 gacgttctcg ctggtcctga ccctgccggt cgggatgatc catgccggcg ccgcgctgtg
     3241 ggcgcccgcg atcctgccgt gggccgtggc ggtggcggtg ctgtcgagcg cgatccccta
     3301 ttcgctggag atgatggcgc tcaggcacat tccggccaag accttcggcc tgatgatgag
     3361 cctcgaaccg gcggcgggcg cgacgctcgg ctttttgatc gtcggggaaa ggctgagcct
     3421 gacccaggtc gcggccatcg cgctggtcat cgtcgcctcg gcgggctcca gcctgaccgc
     3481 gcgccgcccg gtggacgaaa gcctgcc
//
LOCUS       DASVLL010000526         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_467002,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000526 DASVLL010000000
VERSION     DASVLL010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467002"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VF402_08680"
     CDS             complement(<1..494)
                     /locus_tag="VF402_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX7800410.1"
                     /translation="MASDGIESKMPGTNFRPGTAREIVDETATNLIELTGVIKWFDVA
                     KGFGFIVPDNGMPDILLHVTCLRRDGYQAAYEGARVVVEVLERQKGLQAFRIVSMDES
                     TAIHPAQMPPPRTHVTVTPTSGLERAQVKWFNRLRGFGFLTRGDGTPDIFVHMETLRR
                     YGIA"
     gene            complement(639..1400)
                     /locus_tag="VF402_08685"
     CDS             complement(639..1400)
                     /locus_tag="VF402_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX7800411.1"
                     /translation="MIADNLETAVDRLRELVADASMILPFTGAGISTECGIPDFRSPG
                     GFWARNKPIQFEDFLNSREMRDESWRRRFVMEDSFGGAKPGRGHRALASLYKAGKAQA
                     VVTQNIDNLHQASGVAAEHVVELHGNTTYATCLECGRRYEIAEVRARFEANGGHAPDC
                     ACGGFIKTATVSFGQAMPEPAMRRAEALTRDCDLFLVVGSSLVVWPAAGFPALAKRNG
                     AALVIVNREPTEFDEFADLVIRHDIGDALAPFIAH"
     gene            1461..1916
                     /locus_tag="VF402_08690"
     CDS             1461..1916
                     /locus_tag="VF402_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7800412.1"
                     /translation="MRYLHTMLRVRNLDAALDFYCNKLGLKETRRRVDEKNRYTLVFL
                     AGSEDQGLVEDSKKKGRPGPEVELTYNWDTEDYGEARFFGHLAYEVDDIYETCDRLMK
                     AGVTINRPPRDGVMAFVRSPDNHSIELLQKGQPKPAQEPWASMKNTGRW"
     gene            1992..2067
                     /locus_tag="VF402_08695"
     tRNA            1992..2067
                     /locus_tag="VF402_08695"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2026..2028,aa:Glu,seq:ttc)
     gene            2170..2325
                     /locus_tag="VF402_08700"
     CDS             2170..2325
                     /locus_tag="VF402_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800413.1"
                     /translation="MASADPKDKSRVCPACGGRLLVMVRLKASEARAASAYFKCERCA
                     HILIAED"
     gene            complement(2400..2840)
                     /locus_tag="VF402_08705"
     CDS             complement(2400..2840)
                     /locus_tag="VF402_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800414.1"
                     /translation="MTKTKLWTAVALVGAIVMAASPSYARDGRNAAAAIGFGAGALVG
                     AAAANAANNNAYYSPGYYESGYAYEPGYAYDPGYVEEPAYAYEPAPVVAYGSGSGWDR
                     GGQCWISTDQSRGFGYYGPCAQHNEDQFNARQGDFRTVRSRVKP"
     gene            3513..3656
                     /locus_tag="VF402_08710"
     CDS             3513..3656
                     /locus_tag="VF402_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800415.1"
                     /translation="MLNRETMVAALRRDVTFVWHAVIAVVFIGFASLAVAAALLDLLS
                     LQR"
     gene            complement(3663..>4375)
                     /locus_tag="VF402_08715"
     CDS             complement(3663..>4375)
                     /locus_tag="VF402_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEX7800416.1"
                     /translation="EMVGPGKPNALSTQARTRALAYISSHPEIWEVILTGGDPLVASA
                     RRLSDTLRALAAIDHVKVLRIHTRMPIAAPETITPALVRALKVKAKPTWLALHVNHAR
                     ELGRDQRAAIARLADAGIPLLGQTVLLKGVNDTVESLEALMRALVECRIKPYYLHHAD
                     LAPGTAHLRTDIATGQALMRALRGRVSGLCQPDYVLDIPGGHGKAPIGPNYLKSAQTG
                     VAVTDFTGAAHAYPPAAD"
BASE COUNT          734 a         1454 c         1414 g          773 t
ORIGIN      
        1 tcagcaatgc cgtagcggcg cagcgtctcc atgtgcacga agatgtccgg cgtgccgtcg
       61 ccgcgggtca ggaagccgaa gccgcgcagc cggttgaacc atttcacctg cgcgcgctcg
      121 aggccgctgg tcggcgtcac cgtcacatgc gtgcgcggcg gcggcatctg cgccggatgg
      181 atcgcggtcg actcgtccat cgacacgata cggaatgcct gcagaccctt ctgccgctcg
      241 agcacttcga cgacgacgcg cgcgccctcg taggcggcct gatagccgtc gcggcgcagg
      301 cacgtcacgt gcaagaggat atccggcatg ccgttgtcgg ggacgatgaa accgaagccc
      361 ttcgcgacgt caaaccactt gatcactccg gtcagctcga tgaggttcgt cgcggtttcg
      421 tcgacgatct ctctggcggt ccctggccgg aaatttgtcc caggcatttt cgattcaatc
      481 ccgtccgacg ccatgattgc cccgacgtcg ctgcctgctg gcgcctcttg aagagcgctt
      541 cgtcgaatct tgtgaatcga agataacatc cggccttaat ccgcaaaggc caaaataggc
      601 ttgcgcacaa gctgtgcaca gcgtcagaca tttgcgaatc aatgggcgat gaagggggcg
      661 agcgcgtcgc caatgtcgtg acgaatgacg agatcggcga attcgtcgaa ctcggtcggc
      721 tcacggttga cgatcacgag agccgcaccg ttgcgcttgg ccagcgccgg gaagcctgca
      781 gccggccaca ccacaagcga cgagccgacc acgagaaaca gatcgcagtc gcgcgtcaaa
      841 gcctcggcgc ggcgcatcgc cggctccggc atggcctgcc cgaacgacac ggtcgcggtc
      901 ttgatgaaac cgccgcaggc gcagtctggc gcatgtcccc cgttcgcctc gaagcgcgcc
      961 cggacctcgg cgatctcata gcgccgcccg cactcgaggc aggtggcgta ggtcgtgttt
     1021 ccgtgcagtt cgactacgtg ctcggccgca actccggatg cctggtgcag gttgtcgatg
     1081 ttctgcgtga cgaccgcctg cgccttgccg gccttgtaaa ggctggcgag cgcccggtgc
     1141 ccgcggcccg gcttggcccc gccgaaactg tcttccatga cgaagcgccg ccgccaggat
     1201 tcgtcgcgca tctcacgact gttgaggaaa tcctcgaact ggatcggctt gttgcgggcc
     1261 cagaacccgc cgggggaccg gaagtccggg atgccgcact cggtcgagat gccggcgccg
     1321 gtgaacggta ggatcatgct tgcgtccgcg accagctcgc gaagccgatc gacggcggtc
     1381 tcaagattgt cggcgatcat ttgctatata gtgccggcgc ggcgccaacc gcgacccgat
     1441 cccttccgtg aggtttgtcc atgcgatatc tgcacaccat gttgcgcgtc cgcaatctcg
     1501 atgcggcgct cgatttctat tgcaacaagc tcggcctgaa ggagacccgg cgccgggtcg
     1561 acgagaagaa ccgttatacg ctggtgttcc tggcaggcag cgaagatcag ggtctggttg
     1621 aggacagtaa aaagaagggc cgccccggtc ccgaggtcga gctcacctac aactgggaca
     1681 cggaagacta cggcgaggcg cgctttttcg gccatctcgc ctacgaggtc gacgacattt
     1741 acgaaacctg cgaccggctg atgaaggccg gcgtcacgat caaccggccg ccgcgcgacg
     1801 gcgtcatggc cttcgtgcgc tcgcccgaca accattcgat cgaactcctg cagaagggcc
     1861 agcccaagcc ggcgcaggaa ccctgggcgt cgatgaagaa caccggacgc tggtagcgcg
     1921 cttgcgggcc gtggcgaggc tctttatacg gagccatccg ctgcgccggc ctccccggcc
     1981 ccgcaggcca ggcgcccttc gtctagaggc ctaggacacc gccctttcac ggcggcgaca
     2041 cgggttcgaa tcccgtaggg cgcgccatct ctcgcggcca aaaaagcttc gaacaccgga
     2101 agccatgcgc cgccggcagg cctgccagcc tgaacgcagg gtaatacctt ccgcggtgtt
     2161 cattatggga tggccagcgc tgatcccaag gacaagagcc gtgtttgccc ggcctgcggc
     2221 ggacgcctgt tggtaatggt gcggctcaag gcaagcgagg cccgcgccgc gtccgcctat
     2281 ttcaagtgcg agcgttgcgc ccacatcctc atcgccgagg actagtccgc acgcgatgtt
     2341 ccaatgacaa acggcggccg ttgccggccg ccgttgtcgc tctcggccgc gccgctgact
     2401 tacggcttga cgcggctgcg gacggtgcgg aaatcgcctt ggcgggcgtt gaactgatcc
     2461 tcgttgtgct gcgcgcaggg gccgtagtag ccgaagccgc gcgactggtc ggtcgagatc
     2521 cagcactggc cgccgcgatc ccagcccgag ccagaaccgt aggccacgac cggcgcgggc
     2581 tcatacgcat aggccggctc ttcgacgtaa cccggatcgt aggcatagcc cggctcatag
     2641 gcgtagcccg actcgtaata gcccggactg taataggcat tgttgttcgc cgcattggcg
     2701 gccgctgcgc cgaccagcgc gcccgcgccg aagccgatcg ccgccgccgc gttgcgccca
     2761 tcgcgggcgt agctgggact cgccgccatc acaatggcgc cgacgagcgc gaccgccgtc
     2821 cagagtttcg tcttggtcat aggtagcctc cttgtttgtc ctgctttgcc ggaacgtgtt
     2881 cgctcggttt ccggccgttg caatgccgcg gcgcgccgtt ggcattcgaa aatctaaagt
     2941 cagggcccgg acgatcgttc cggtgctggc gcacctgtga gatcggggcc ggagtggctc
     3001 agcaatggcg cggccgtgcg gcgctggatg caacatcaac gcgcaatgcg cgttggcttt
     3061 ccgctccact ttcgggacgg cccatgcgat tccttctggc ggcggcagtg ttcgtgacgg
     3121 cgctggcggt cgatgtgcgg cccgcaccgg cgcgcgtcgc tcacgtggcg gcgccatggt
     3181 gtgtcattca caacaccggc cgcgccgagc ggacgtgctt tccgacgcgc gcgctctgtc
     3241 accgcttcgg cgaacgaccc ggcggcggcg ccgaatattg cgtccggtat cccgtgtgga
     3301 gcggccgacg ctaggcctcg tcacgcggca caacgcgacg accgaagcct tcctgcgctc
     3361 cttcacgaat tcgtgcgtcg ctgcggcgtt atgtcagcca tgcgcgacga taagcgcaat
     3421 tcccgcggat ttaacgcact cgttatgtgg cggaacgtac cgcggatgta cattccgcct
     3481 tgccctgcct tcgctgcaag gagcatcggg ccatgctgaa cagagagacc atggtagctg
     3541 cgcttcgccg cgacgtgacc ttcgtctggc acgccgtgat cgctgtcgtc ttcatcggct
     3601 tcgccagcct cgccgtcgcc gccgcgctgc tcgacttgct gtcgttgcag cgctagagcg
     3661 cctcaatccg ccgcgggcgg ataggcgtgc gccgcgcccg tgaagtccgt caccgccact
     3721 cccgtttgcg ccgatttgag ataattcgga ccgatcggcg ccttgccatg gccgccggga
     3781 atatcgagca catagtctgg ctggcacagg cccgacacgc ggccgcgcag cgcccgcatc
     3841 aaggcctgcc ccgttgcgat atccgtacgc agatgcgcag tgccgggcgc cagatcggcg
     3901 tgatgcagat aataaggctt gatgcggcat tccacgagcg cgcgcatcaa ggcctcgagc
     3961 gattcgaccg tgtcgttgac gcccttcaaa agcaccgtct ggccgaggag cggaatgccg
     4021 gcgtcggcga gccgcgcgat cgccgcgcgc tgatcgcggc cgagctcgcg cgcatggttg
     4081 acgtgcaggg cgagccaggt cggcttcgct ttgaccttca gcgcccgcac cagcgccggg
     4141 gtgatcgtct caggcgccgc gatcggcatg cgcgtgtgga tgcgcagcac cttgacgtga
     4201 tcgatggcgg cgagtgcacg cagcgtatcg gagagccgcc tcgccgaggc gacgagcggg
     4261 tcgccgcccg tcaggatcac ctcccagatc tcaggatgcg acgagatata ggcgagcgcg
     4321 cgggtgcgcg cctgtgtcga cagcgcattc ggcttgcccg gcccgaccat ttcgc
//
LOCUS       DASVLL010000527         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_250351,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000527 DASVLL010000000
VERSION     DASVLL010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250351"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VF402_08720"
     CDS             complement(<1..760)
                     /locus_tag="VF402_08720"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7800417.1"
                     /translation="MSQQVRQFTVEPDDDGIRLDRWFKRHLPQVGFGTVSKWARTGQL
                     RVDGKRVKPEDRLAAGQVLRVPPGGDTEPKKREPRSLTETQRAEAEAMLIAKTDAALV
                     LNKPPGLATQGGTKTYNHVDGLLDAYADEDEPRPRLVHRLDKDTSGVLLVARTPGSAA
                     FFSKRFSGRSARKVYWALVVGVPEIAEGLIDAPIAKQPGTGGEKMHVDEEGGQAARTR
                     YRVVDRAGNRAAWLELEPLTGRTHQLRVHCAAMGH"
     gene            complement(757..1164)
                     /gene="crcB"
                     /locus_tag="VF402_08725"
     CDS             complement(757..1164)
                     /gene="crcB"
                     /locus_tag="VF402_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX7800418.1"
                     /translation="MNPPTQILASSYVALGGAIGALLRYQSGRLMTHWLGAAVVTAFP
                     WATLVVNVVGSLFMGALAGFLARHGHGGEQWRLFIGVGVLGGFTTFSSFSLEMMLLVE
                     RGQAFQALTYALVSILAGLTALYLGLIIMRIAG"
     gene            1371..1577
                     /gene="rpsU"
                     /locus_tag="VF402_08730"
     CDS             1371..1577
                     /gene="rpsU"
                     /locus_tag="VF402_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7800419.1"
                     /translation="MQIMVRDNNVEQALRALKKKLQREGVYREMKLRRHYEKPSEKRA
                     REKAAAVRRARKLERKRAERDGSK"
     gene            1653..2228
                     /locus_tag="VF402_08735"
     CDS             1653..2228
                     /locus_tag="VF402_08735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7800420.1"
                     /translation="MVEPTRVPLQPIRKGSLTKVWAGIVVVLLVAAAFAWFNVPHAKK
                     LAGGVTLETLQEGKGPNPTTSDIALINYKGMLANGKAFDQGQGVPMPVTGVVPGFSTG
                     LQAMQAGGKYRLRIPAEQAYGSEEKRNQQTGEVVIPANSDLVFDVDLLEFRSQEEVMR
                     MMQQQQMMQQLQQGGAGGASGAPAPAPAPGQ"
BASE COUNT          412 a          760 c          768 g          396 t
ORIGIN      
        1 gatgccccat cgccgcgcag tggacgcgca gttgatgggt gcggccggtg agcggctcga
       61 gttccagcca ggcggcgcgg ttgccggcgc ggtcgaccac ccggtagcgg gtccgggcgg
      121 cctgaccgcc ttcttcgtcg acatgcatct tttcgccacc ggtgcccggc tgcttggcga
      181 tcggcgcgtc gatcagtccc tcggcaatct ccggcacgcc gacgacgagc gcccaataaa
      241 ccttcctggc gctgcgccct gagaagcgtt tcgagaagaa cgccgcgctg cccggcgtgc
      301 gcgcgacgag caggactccg ctggtgtcct tgtcgagccg gtggaccagg cgcggacgag
      361 gttcgtcctc gtccgcatag gcatcgagca ggccatcgac gtggttgtac gtcttggtcc
      421 cgccctgcgt cgccagaccc ggcggtttgt tgagcaccag tgcggcgtcg gtctttgcga
      481 tcagcatcgc ttcggcctcc gcccgctgcg tttcggtcag tgacctcggc tcgcgtttct
      541 tcggttccgt gtcgccgccc ggcggcacac gcaggacctg acccgccgcg agccggtctt
      601 ccggcttcac ccgcttgccg tctacccgca gttgcccggt tcgcgcccac ttggaaaccg
      661 tgccgaagcc cacttgcggc aggtggcgct tgaaccagcg atcgagcctg atgccgtcat
      721 cgtcgggctc gaccgtgaat tgccggactt gctggctcat cccgcgatgc gcatgatgat
      781 caggcccagg tagagcgccg tcaggccggc cagtatcgaa acgagcgcgt aagtcagggc
      841 ctggaaggcc tgtccgcgct cgaccagcag catcatctcc aggctgaagc tggaaaaggt
      901 cgtgaagccg ccgagcacgc ccacgccgat gaacagccgc cactgctcgc cgccgtgccc
      961 gtggcgagcg agaaacccgg cgagcgcgcc catgaacagg ctgccgacca cgttgaccac
     1021 caaggtcgcc caagggaaag ccgtcaccac ggcggccccc agccagtggg tcatcagccg
     1081 gccgctctgg tagcgaagca gggctccgat cgcgccgccg agagcgacgt aggaggaggc
     1141 gagaatttgg gtgggcgggt tcatccccgg ctctagccgc ggcgaagcgc ggttggaagg
     1201 cgttccgccc cgaaatcctt gcctttcccg cccgtttcgc ttatcgggcg ccccgttctg
     1261 ccggtcccct gtggattcgg ccgctttttc atttggtttt acgctggtgc gcctgctccg
     1321 gacgatgcgg gctcttaccg gatttgaaga gacaagaggt agcttagttt atgcagatca
     1381 tggttcgcga taacaatgtc gagcaggccc tgcgcgcgct gaaaaagaag ctgcagcggg
     1441 aaggcgtcta tcgtgaaatg aagctgcgcc gccactacga gaagccgagc gagaagcgcg
     1501 cccgcgaaaa ggccgccgcc gtacgccgtg cgcgcaagct cgaacgcaag cgggcggagc
     1561 gcgacggcag caagtaagct tgtaagccga ctggcgataa gccaaagggg cgcgggacat
     1621 ctcccgcgcc ttttttgcat caggaaccca caatggtcga acccacccgt gtcccgcttc
     1681 agccgatcag gaagggctcg ctgaccaagg tctgggccgg catcgtcgtc gtgctgctgg
     1741 tggcggccgc cttcgcctgg ttcaacgttc cgcacgcgaa gaagctcgcc ggcggcgtga
     1801 cgctggaaac gctgcaggaa ggcaaggggc cgaacccgac gacctccgat attgcgctga
     1861 tcaactacaa gggcatgctc gctaacggga aggcattcga ccaggggcag ggcgtgccga
     1921 tgcccgttac cggcgtggtg cccgggttct cgaccgggct ccaggcgatg caggcgggcg
     1981 gcaagtaccg cctccgcatt cccgcggagc aggcctacgg ttccgaagaa aaacgcaatc
     2041 agcaaaccgg cgaagtggtt attccggcga acagcgatct ggttttcgac gtcgacctgc
     2101 tggaattccg tagccaggaa gaagtcatgc ggatgatgca gcaacagcag atgatgcagc
     2161 agttgcagca agggggcgcc ggcggtgcct cgggcgcgcc cgcgcccgca cctgcgccgg
     2221 ggcaataagc cgcccaacag ctagtccaag atgacgattg aggggattcc gtgcctaatc
     2281 ggcgacgtcc ctcccaggtt ccttgacagg gggaaggtcg aattgccgat tcctca
//
LOCUS       DASVLL010000528         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_145613,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000528 DASVLL010000000
VERSION     DASVLL010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145613"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="gltX"
                     /locus_tag="VF402_08740"
     CDS             complement(<1..974)
                     /gene="gltX"
                     /locus_tag="VF402_08740"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7800421.1"
                     /translation="MSDTPSQVVTRFAPSPTGFLHIGGARTALFNWLFARHHGGKFLL
                     RIEDTDRARSTQPAIDAILDGMTWLGLDWDGEAVHQFARAERHVEVAGQLLAGGHAYK
                     CFATPEELAALREEQRAQGKPIRYDGRWRDRDPAQTPEGAPFVVRLKAPREGATTIVD
                     RVQGEVTVQNSEIDDFVLLRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHLNNAFRQLP
                     IIRAMNWPEPVYAHIPLIHGPDGAKLSKRHGALGVDAYRDELGILPEALDNYLLRLGW
                     GHGDDEIISRGQAIDWFELDAVGKSPSRFDLKKLENLNGHYIREAGDAR"
     gene            1057..3201
                     /locus_tag="VF402_08745"
     CDS             1057..3201
                     /locus_tag="VF402_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX7800422.1"
                     /translation="MKTAVWRAVEAAWTQQAGRLFLWLPVAFGAGAALYLALPVEPSW
                     AWCAGPCAVSLLAAMLCRRWAAPGWLGGLTLVLFLFALGVTTAKLRAEHVAAPVLDPD
                     RTTYRLTTFVVDNISPSREEPRLLLAPIAMPGVAPERTPLRLRVSLRPGVIEAAHVRP
                     GDAISVFAILNPPPAPSLPGGYDFAQAAWFQGIGGAGFVPGAVRTLAIKPAGFRLSYV
                     LWLNRLRWAITQRLVNAITPVWRDGGELGGFAAALVTGQQAYVPQSLIVDMRNSGLAH
                     ILSISGVHMAVVGGFVFFGLRWLMAWFPPLVLRFPVKKIVAALSMGCVLLYLAVSGAP
                     APAVRSAVVACVAFAAILLDRRALSLRALAIAAFVVIALTPEAVIQPGFLMSFTATAA
                     LLALAEGWRPPIREISVPGWVRAIQSAIQAVWLSILASLVATAATTPFAIAYFNRFSV
                     YGLLSNLFESPITAFVVMPFLALGTVLAGTPLGPVCLNIAAGGLWALRRIAALTSALP
                     HAVIGWPTAPAFILPLATMGVVWACLVKGRMRWLGAVAASAILWWPHTPPSDIWIDPA
                     GGNAAVRVGGEAFVLRPKVRQYGFVLWTQRYGLTASPDARDGYYACKGYACVPKAGAP
                     FRVGFWFSNKPPKAAKFDELCSASDLVVLRNPMADWPASCAGVNRISAGDFRRLGAME
                     LTRQTKGWAIRAAQPLRGHRFWSVRSDADSDQ"
     gene            complement(3195..3893)
                     /gene="lexA"
                     /locus_tag="VF402_08750"
     CDS             complement(3195..3893)
                     /gene="lexA"
                     /locus_tag="VF402_08750"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX7800423.1"
                     /translation="MLTTKQRELLMFIHERIKETGVSPSFDEMKEALDLASKSGIHRL
                     ITALEERGFIRRLAHRARALEVIKLPDQATASAPPRGRQAFVPQVVEGARPSEAPRAV
                     NDDARDLPLLGKIAAGVPIEAMGQERDRMPVPEAMLGAGEHYILEIEGDSMINAGILN
                     GDYVVIRKTDSAQSGEIVVALVDDVTATLKRLRRKGASIALEAANPAYKTQIYNDGQV
                     KIQGRLVGLMRRYH"
BASE COUNT          522 a         1263 c         1426 g          749 t
ORIGIN      
        1 cgcgcatcgc cggcctcgcg aatgtagtga ccgttaaggt tctccagctt cttcaaatcg
       61 aaccgcgatg gtgatttgcc gactgcatcg agctcgaacc agtctatcgc ctgcccgcga
      121 ctgatgatct cgtcgtcgcc atgaccccag ccgaggcgga gcaggtagtt gtcgagcgcc
      181 tccgggagga tgccgagttc gtcgcgatag gcatcgacgc ccagcgcacc gtggcgcttg
      241 gagagcttgg cgccgtcggg accgtggatc agcgggatat gcgcatagac cggctcgggc
      301 cagttcatcg cgcggatgat gggcagctga cggaaggcgt tgttgagatg gtcgtcgccg
      361 cggatgacgt gggtgacgcc catatcgtgg tcgtccacca ccaccgcgag catgtaggtc
      421 ggggtgccgt cggagcggag caggacgaaa tcgtcgatct cgctgttctg gacggtgacc
      481 tcgccctgca cgcggtcgac gatcgtcgtc gcgccttcgc gcggcgcctt gaggcggacg
      541 acgaacggcg cgccttcggg cgtttgggcg gggtcgcggt cgcgccagcg gccgtcgtag
      601 cggatcggct tgccttgggc gcgctgttcc tcgcgcagcg cagcgagctc ctcgggcgtc
      661 gcaaagcatt tgtaggcgtg gccgccggcg agcagttgtc cggcgacctc gacgtgacgc
      721 tcggcgcgag cgaactggtg gaccgcctcg ccgtcccaat cgaggccgag ccatgtcatg
      781 ccgtcgagga tcgcgtcgat cgcgggctgg gtggagcgag cgcggtcggt gtcctcgatc
      841 cgcagcagga atttgccgcc atgatggcgc gcgaacagcc agttgaacag cgcggtgcgc
      901 gcgccgccga tatgcaggaa accggtcggc gagggggcga agcgggtgac gacttgcgaa
      961 ggcgtatcgg acatggtcat gtagctcgga tttcgatttg gtctgcgtca tatagcgccg
     1021 cgcgcgcgca accaagtctt aaacacgggc ggggggatga aaacagcggt ttggcgggct
     1081 gtcgaggcgg cgtggacgca gcaggcgggg cggttgttcc tgtggctgcc ggtggcgttc
     1141 ggcgcgggcg cggccctgta tctggcgctg ccggtcgagc cgtcctgggc atggtgcgcc
     1201 ggtccgtgcg cggtgtcgtt gctggcggcg atgctgtgcc ggcggtgggc ggcgccgggc
     1261 tggctcggcg gattgacgct tgtgctgttc ctgttcgcgc tgggcgtgac gacggcgaag
     1321 ctgcgcgccg agcatgtcgc ggcgccggtt ctggatcccg accggacgac ctaccgcctg
     1381 accaccttcg tggtcgacaa tatctcgccg tcgcgcgagg agccgcgtct gctgctggcg
     1441 cccatcgcca tgccgggcgt cgcgccggaa cggacgccgc tgcgcttgcg cgtttcgctg
     1501 cgtccgggcg tgatcgaggc cgcccatgtg cggcccggcg atgcgatctc ggtgttcgcg
     1561 atcctgaacc cgccgcccgc gccgtccttg cccggcggct acgatttcgc gcaggccgcg
     1621 tggtttcagg gcataggcgg cgcgggcttc gttccgggcg cggtgcgcac gctggccatc
     1681 aagcccgccg ggtttcgcct gagttatgtc ttgtggctga accggctgcg ctgggcgatc
     1741 acgcagcggc tggtgaacgc gatcacgccg gtgtggcgcg acggcggcga actgggcggc
     1801 ttcgcggcgg ctctggtgac ggggcagcag gcctatgtgc cgcaaagcct gatcgtggac
     1861 atgcgcaatt cggggctggc gcatatcctg tcgatttccg gcgtgcatat ggcggtggtc
     1921 ggcggcttcg ttttcttcgg cctgcgctgg ctgatggcgt ggtttccgcc gctggtcctg
     1981 cggtttccgg tcaagaagat cgtcgccgcg ctgagcatgg gatgcgtgct gctgtatctg
     2041 gcggtgtcgg gcgcgcccgc gccggcggtg cgctcggcgg tcgtggcctg cgtggcgttc
     2101 gcggcgattt tgctggaccg cagggcgttg tcgttgcggg cgctggccat cgcggcgttc
     2161 gtggtgatcg cgctgacgcc cgaagcggtg atccagcccg gcttcctgat gtcgttcacc
     2221 gccacggcgg cgctgctggc gctggccgag ggctggcggc cgccgatccg cgaaatcagc
     2281 gtgcccggct gggtcagggc cattcaatcg gcgattcagg ccgtctggct gtcgattctg
     2341 gcgtcgctgg tcgccaccgc cgcgacgacg ccgttcgcca tcgcctattt caaccgcttc
     2401 tcggtctatg gcctgttgtc caacctgttc gagtcgccga tcaccgcctt tgtcgtcatg
     2461 ccgtttctgg cgctcggcac ggtgctggcg gggacgccgc tggggccggt ctgcctcaat
     2521 atcgccgcgg gcggactatg ggcgctgcgc cggatcgccg ccctgacctc ggcgctgccg
     2581 cacgccgtca tcggctggcc gacggcgccg gctttcattc tgccgctggc gacgatgggc
     2641 gtggtgtggg cctgcctcgt caaggggcgg atgcgatggt tgggcgccgt cgcggccagc
     2701 gcgatcctgt ggtggccgca cacgccgccg agcgacatct ggatcgatcc ggcgggcggc
     2761 aatgcggcgg tgcgcgtcgg cggggaggct ttcgtgctgc gtcccaaggt gcggcaatac
     2821 ggcttcgtgc tgtggacgca gcgctatggc cttacggcgt cccccgatgc gcgcgacggc
     2881 tattacgcgt gcaagggcta tgcctgcgtt ccgaaggccg gggcgccgtt ccgggtcgga
     2941 ttctggttct ccaacaagcc gccgaaagcc gccaaattcg atgaattgtg ttcggcaagc
     3001 gatctggtcg tgctgcgcaa cccgatggcc gactggcccg cctcgtgcgc aggcgtcaac
     3061 cgcatcagcg ccggggattt ccgccgcctc ggcgcgatgg agctgacgcg acaaacaaaa
     3121 ggctgggcga tccgcgccgc ccagcccctc agaggtcatc gtttctggtc cgtgcgttcg
     3181 gacgccgatt ccgatcagtg ataacggcgc atcaggccga cgaggcggcc ctgaatcttc
     3241 acctgaccgt cattgtagat ctgcgtctta taggccgggt tggcggcttc gagcgcgatc
     3301 gacgcgccct tgcggcgcag gcgtttcaac gtcgccgtga cgtcgtcgac cagcgccacc
     3361 acgatctcgc ccgactgcgc ggaatcggtc ttgcggatca cgacgtaatc gccgttgaga
     3421 atgcccgcat tgatcatcga atcgccttcg atttcgagga tatagtgttc gcccgcgccg
     3481 agcatggctt ccggcaccgg catgcggtcg cgctcctggc ccatcgcctc gatcggcacg
     3541 cccgccgcga tcttgcccag cagcggcagg tcgcgggcgt cgtcattgac ggcgcgcggc
     3601 gcttcggacg gcctggcgcc ctcgaccacc tgcggcacga aggcctgacg gccgcgcggc
     3661 ggcgccgagg cggtggcctg atcgggcagt ttgatgactt ccagcgcccg cgcccggtgg
     3721 gcgaggcggc ggatgaagcc gcgttcctcc agcgccgtga tcaggcggtg aatccccgac
     3781 ttggaggcca gatccagcgc ctccttcatt tcgtcgaacg agggcgatac gcccgtctcc
     3841 ttgatgcgtt cgtgaatgaa catcagaagt tcgcgttgtt tggtcgtcag catcgggaca
     3901 tccttcgcat cgcccggcca ccccacgggt tgcgcggaac aagtcatgaa cctgcgaatg
//
LOCUS       DASVLL010000529         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_196892,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000529 DASVLL010000000
VERSION     DASVLL010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196892"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF402_08755"
     CDS             complement(<1..252)
                     /locus_tag="VF402_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX7800424.1"
                     /translation="MATTTTKTIMLVAAEASGDLLGAGLMAQLKAQAPQVDWRFVGVG
                     GARMAEHGVKSPFDIAELSILGLVEGLKAYGRVMRRVRDT"
     gene            complement(233..1075)
                     /gene="lpxI"
                     /locus_tag="VF402_08760"
     CDS             complement(233..1075)
                     /gene="lpxI"
                     /locus_tag="VF402_08760"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080215.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX7800425.1"
                     /translation="MSGGGKLALIAGGGGLPVEIAAYLRAMGRPYIVIRIEALSDEAL
                     DAHPGYTLGLGDFLKLFEILSNEQCRAVCMCGYVQRPDFDAMKRDEMGARVLPTIQSA
                     GRAGDDSLLRQLANVITAQGYVIEGAHEANPDLLIGSGLQAGPDLSPAAMEDAQKALG
                     IAAAIGKLDVGQAVVVAGGVVLAVEAQEGTQAMLRRIPLLPATLRGTAGTRKGVLAKL
                     AKPIQDVRLDMPTIGVATVDDAAEAGLCGIVARSHHLLIVDKPAVFARAAEKGVFIYG
                     HDDN"
     gene            complement(1072..1866)
                     /gene="lpxA"
                     /locus_tag="VF402_08765"
     CDS             complement(1072..1866)
                     /gene="lpxA"
                     /locus_tag="VF402_08765"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX7800426.1"
                     /translation="MTVSIHPTAIVHDGAQLGADVTIGPFCIVGPQVTLKDRVALKSH
                     VSVDGVTELGADCVVHPFACLGGPPQHLAHQGEPTRLVVGERNLIREHVAMHAGTVKG
                     GGVTVIGNDCMFMTGSNVAHDCILGDNVIMANYASLGGHVRVGDFVFLGGLSAVHQFA
                     RLGRYCFIGGGGIVTKDVIPYGSVWGNHARLEGLNLVGLKRRGFSRELILALRTAYRM
                     MFAEEGTFQERLDDVLENFSEVDQVVEIVRFIREDSTRPICLPSDS"
     gene            complement(1863..2375)
                     /gene="fabZ"
                     /locus_tag="VF402_08770"
     CDS             complement(1863..2375)
                     /gene="fabZ"
                     /locus_tag="VF402_08770"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX7800427.1"
                     /translation="MTNLTDEAALNAGEGEGENAAAPVHVDYAEILRRIPHRYPFLLI
                     DRGENWIRNKSMTGIKNVTFNEPFFNGHFPENPVMPGVLLIEALGQTGAVLMSKSLDA
                     QVEGKTIFFMSVDGVRFRSPVRPGDVVRMPVEVVRHRGDVFKFRGEAYVGDRIVCEAE
                     FAAMVVESPK"
BASE COUNT          416 a          872 c          764 g          403 t
ORIGIN      
        1 cgtatcgcgc acccgccgca tcacgcggcc ataggctttc aggccctcca cgaggcccag
       61 aatcgacagt tcggcgatgt cgaacgggct tttcaccccg tgttcggcca tgcgcgcgcc
      121 gccgacgccg acgaagcgcc agtcgacctg cggcgcctgc gccttcaact gcgccatcag
      181 cccggcgccg agcagatcgc cggacgcctc ggcggcgacc agcatgatcg tcttagttgt
      241 cgtcgtggcc atagatgaag acgcctttct cggcggcgcg ggcgaaaacg gccggtttat
      301 ccacgatcag caggtgatgg gaacgcgcca cgatgccgca caggcccgcc tcggccgcgt
      361 cgtccaccgt cgcgacgccg atggtcggca tgtcgagccg cacgtcctga atcggcttgg
      421 ccagcttggc cagcacgccc ttgcgcgtcc cggccgtccc gcgcaacgtc gcgggcagca
      481 gcggaatccg ccgcagcatg gcctgcgtcc cctcctgcgc ctcgaccgcc aggaccacgc
      541 cgcccgcgac gacgaccgcc tgcccgacgt cgagcttgcc gatggcggcg gcgatgccga
      601 gggccttctg cgcgtcctcc atcgcggcgg gcgacaggtc cggcccggcc tgcaagccgg
      661 agccgatcag caagtccggg ttggcctcgt gcgcgccttc gatgacatag ccctgcgcgg
      721 tgatgacgtt ggccagttgc cgcagcagcg aatcgtcgcc cgcgcgcccg gcgctctgga
      781 tcgtcggcag cacgcgcgcg cccatctcgt cgcgcttcat cgcgtcgaaa tcggggcgct
      841 gcacatagcc gcacatgcac acggcgcggc actgttcgtt cgacaggatc tcgaacagct
      901 tgagaaaatc gcccagcccc agcgtgtagc cgggatgcgc gtccagcgcc tcatccgaca
      961 gcgcctcgat gcggatgacg atatagggcc gccccatcgc gcgcagatac gccgcgatct
     1021 cgaccggcag gccgccgccg cccgcgatca gggccagttt gccgccgccg ctcatgagtc
     1081 ggacggcagg cagatcggac gggtggagtc ctcgcggatg aagcgcacga tctcgaccac
     1141 ctgatcgacc tcggagaagt tctccagcac gtcgtcgagg cgctcctgaa acgtgccttc
     1201 ctcggcgaac atcatgcggt aggcggtgcg cagcgccagg atcagctcgc gcgagaagcc
     1261 gcgccgcttg agcccgacca gattcagccc ttccagccgc gcatggttgc cccacacgct
     1321 gccgtagggg atcacgtcct tggtgacgat gccgccgccg ccgatgaagc agtaacgccc
     1381 cagccgcgcg aactgatgca ccgccgacag cccgcccaga aacacgaagt cgccgacgcg
     1441 cacatgcccg cccagcgagg cgtaattggc catgatgacg ttgtcgccga ggatgcagtc
     1501 gtgcgcgacg ttcgagccgg tcatgaacat gcagtcgttg ccgatcaccg tcacgccgcc
     1561 gcccttgacc gtgccggcgt gcatggcgac gtgttcgcgg atcaggttgc gctcgcccac
     1621 gaccagcctg gtcggctcgc cctgatgcgc cagatgctgc ggcggcccgc ccaggcaggc
     1681 gaacggatgc acgacgcaat ccgcgcccag ctccgtcacc ccgtccaccg agacgtgcga
     1741 tttcagcgcg acgcggtctt tcagcgtcac ctgcggcccg acgatgcaga aaggtccgat
     1801 cgtcacgtcg gcgcccagtt gcgcgccgtc atggacgatg gccgtcgggt gtatgctgac
     1861 tgtcatttag ggctctcaac caccatggcc gcgaattcgg cctcgcagac gatccggtcg
     1921 ccgacatagg cttcgccgcg gaatttaaag acgtcgccgc gatggcgcac gacctcgacc
     1981 ggcatgcgca ccacgtcgcc cggacgcacc gggctgcgga aacgtacacc atccacggac
     2041 ataaagaaga tggtcttgcc ttcaacttgc gcgtccaaag atttcgacat cagcaccgcg
     2101 ccggtctggc ccagggcctc gatcagcagc acgccgggca tgaccgggtt ttccgggaag
     2161 tgcccattga agaacggctc gttgaacgtc acgttcttga tgccggtcat cgacttgttc
     2221 ctgatccagt tttcgccgcg gtcgatcagc aggaacggat agcggtgcgg gatgcggcgc
     2281 agaatctcgg cgtaatccac atgaaccggc gcggccgcgt tctccccctc gccttcgccg
     2341 gcgttcaacg cggcttcgtc agtcaaattc gtcatgaagg ttgattatcc tttatccttg
     2401 atggcgttct tctccagcca gaccatttcg cgcaggaact ggcgcgacgg ccgtg
//
LOCUS       DASVLL010000530         5235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_250489,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000530 DASVLL010000000
VERSION     DASVLL010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250489"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VF402_08775"
     CDS             <1..1362
                     /locus_tag="VF402_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX7800428.1"
                     /translation="HNVLNARHHESEGDIIADAGVPGSVTIATNMAGRGTDIQLGGNV
                     DMRVQKWISDQEAAGVEVTQEQIFEKKGEIEAQVAVLKEKALAAGGLFVLGTERHESR
                     RIDNQLRGRTGRQGDPGRSKFFLSCEDDLMRIFAGDKLNAMMKTLGVEDGEAITSPLL
                     NGAIATAQKRVEQRNYEIRKNLLKYDDVVNDQRKAVFEQRQEFMDTDDLSELIAEFRQ
                     DTIHDLVEQHMPPKAYADQWDIDGLNERVQALTGLDLPLHDWADEEGIANEEFEERLQ
                     KAAAEKMDERLRLLGDEQMKALEKQFLLQMIDMHWREHLMHLDHLRAVIGLRGYGQRD
                     PLNEYKTEAFTMFEALLVNLRQSVTRWLMTMEIRFQAPEEAPPEMPPALSQRRSFQEV
                     HLDPLTGHNERAARLSDDLTPEQRAALPHSALPGGWENTARNGLCPCGSGKKFKHCHG
                     VLV"
     gene            1519..2094
                     /locus_tag="VF402_08780"
     CDS             1519..2094
                     /locus_tag="VF402_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800429.1"
                     /translation="MRIASMTVALGLALTALPAGAQEIVAAAPADRAIAAGDVAAVAV
                     AQIDDKADAAQAQQKIDLYKQLMELNGVTQNIRNVVAATKTATRLIIIDRAGVDKLTP
                     EQDARYNRIADNVLSATQDELIDTVAAAQAPSFTLAEIHQLIDANASPAAARYNAVKF
                     LTPDDNAKAVQGYMVDAVIKIIKSYSQSQTG"
     gene            2120..2809
                     /locus_tag="VF402_08785"
     CDS             2120..2809
                     /locus_tag="VF402_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800430.1"
                     /translation="MTLRLVLYTALAAALIAAPASAQTRPMLSILSLSGPDAKPADIT
                     AGLAAGQGAPSAPSDAALDKQTAEAVTALYENRTADQAARPAADAVADPALAERIGLA
                     RALFRVDGTDALIRHFVETEHMKLIIAEVARHIDFSKLSESDKYRLAAIAANAQAELE
                     DRILNLSAAQTAQLMTKPDLLQLLAAFDTDAQRKLTAVRLGDNGKTDSLAELDIRLAQ
                     YQIVKQYESGQ"
     gene            complement(2832..3548)
                     /locus_tag="VF402_08790"
     CDS             complement(2832..3548)
                     /locus_tag="VF402_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7800431.1"
                     /translation="MSRIRSLLFMLWLYGSMAFFGLVFSPLLLAPPVCALAVIRWWAK
                     AVLFGARLIVGIRVEFRGLEHRPDGAALLAGKHMSMLDTIAPFVVLKAPAYVLKKSLI
                     YLPFFGWYAWRTRMVAIKREDAAKALKSMVAACRGSLAEGRQIVIFPEGTRSDPGDDP
                     DYKPGVAALYRDLQVPCHLLATNSGQFWPGHGVDRKPGTVVFEFLPPLPAGMKRAEFM
                     TEVKARLEGASARLVADNAR"
     gene            complement(3556..4203)
                     /locus_tag="VF402_08795"
     CDS             complement(3556..4203)
                     /locus_tag="VF402_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7800432.1"
                     /translation="MRSLIALGVVVLLWLAGLLVFADRVIDSTPAIEPDAPADAIVVL
                     TGASDLRLKEGMRLLERRKGQRLFISGVNREVTRPQLLHVTEGSRRLYECCVDLGYEA
                     ENTVGNAREIADWARGHDFYDLIVVTSDYHMPRALLELKADMPDAHFIAYPVATPELD
                     ARGWWKSPKGQRLIVLEYCKYLAILGRDAILSVSRAFGDKGEAPKSGSEAAASAS"
     gene            complement(4233..5129)
                     /locus_tag="VF402_08800"
     CDS             complement(4233..5129)
                     /locus_tag="VF402_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7800433.1"
                     /translation="MINFFLDRFRNLQARDLLPRQDEREVALHYVIGVLCFLACMAAL
                     VVMASDRAAAGWSRDIRSEITVQVRPQGLESGSVAAAHAAEALAGIKGVGQVTALEPQ
                     KAKDLIKPWLGDAVLDDLPIPNLVEVSLDPRHPASLHDIREALSAQNIDASVDDHSVW
                     LKDVQQSALTIRLLSLGVFLIIGSATAAVVGFATRAGLAARGNIVEVLSLCGASDIFI
                     AERFQNRFARMAFESGLLGAGLAAVAMALIKVTGRSQSFALALPFSWSDLLILIPCPV
                     LVALIAAVTARMVTLRLLGGGR"
BASE COUNT          980 a         1832 c         1708 g          715 t
ORIGIN      
        1 cacaacgtcc tcaacgcgcg ccaccacgaa tccgaaggcg acatcatcgc cgacgccggc
       61 gtgccgggtt cggtgaccat cgccaccaac atggccggtc gcggcaccga catccagttg
      121 ggcggcaatg tcgacatgcg cgtgcagaag tggatttccg accaggaagc cgcgggcgtc
      181 gaggtcacgc aggagcagat tttcgagaag aagggcgaga tcgaggccca ggtcgccgtg
      241 ctcaaggaaa aggcgctggc ggcgggcggc ctgttcgtgc tgggcaccga gcggcacgaa
      301 agccgccgca tcgacaacca gcttcgcggc cgcaccggcc gtcagggcga ccccggccgc
      361 tcaaaattct tcctgtcgtg cgaagacgac ctgatgcgca tcttcgcggg cgacaagctg
      421 aacgcgatga tgaagacgct gggcgtcgag gacggcgagg cgatcacctc gccgctgctc
      481 aacggcgcca tcgccacggc gcaaaagcgc gtcgagcagc gcaactacga aatccgcaag
      541 aacctgctca aatacgacga cgtggtcaac gaccagcgca aggccgtgtt cgagcagcgt
      601 caggagttca tggacaccga tgacctcagc gaactgatcg ccgaattccg tcaggacacc
      661 atccacgatc tcgtcgagca gcacatgccg cccaaggcct atgccgacca gtgggacatc
      721 gacggcctca acgagcgcgt ccaggccctg accggcctcg acctgccgct gcacgactgg
      781 gccgacgaag aaggcatcgc caacgaggag ttcgaggagc gcctgcaaaa ggccgccgcc
      841 gagaagatgg acgagcgcct gcgccttttg ggcgacgagc agatgaaggc gctggaaaag
      901 cagttcctgt tgcagatgat cgacatgcac tggcgcgagc acctgatgca cctcgaccac
      961 ctgcgcgcgg tgatcggcct gcgcggctac ggccagcgcg atccgctcaa cgagtacaag
     1021 accgaagcct tcaccatgtt cgaggcgctg ctggtcaatc tgcgccagtc ggtcacgcgc
     1081 tggctgatga cgatggagat ccgcttccag gcgccggagg aggcgccgcc ggaaatgccg
     1141 cccgccctgt cgcagcgccg ttccttccag gaagtgcatc tcgacccgct gaccggccac
     1201 aacgagcgcg ccgccaggct cagcgacgat ctgacgccgg aacagcgcgc cgccctgccg
     1261 cactcggccc tgcccggcgg ttgggaaaac accgcccgca acggcctgtg cccctgcggc
     1321 tccggcaaga agttcaaaca ctgccacggc gtactggtgt aaaaatcccc cttctcctcc
     1381 ccattcaagg ggagcgaccg cgaagcggtg gaggggcctt ctcccccccc ccgaaaaaca
     1441 cgccgttacg cgacccgctt gcctgcgcgg ccttaccggg ttaaggtccg gttaagcatc
     1501 gggtgaaggg gagccgccat gcgcatcgca tccatgaccg tcgcgctggg gctggcgctg
     1561 accgccctgc ccgccggggc gcaggagatc gtcgccgccg cgccggcgga ccgggcgatc
     1621 gccgccggcg acgtcgccgc cgtcgccgtg gcccagatcg acgacaaggc cgacgccgcg
     1681 caggcgcagc agaagatcga cctctacaag cagctcatgg agctgaacgg cgtcacccag
     1741 aacatccgca acgtggtcgc cgccaccaag accgccacgc ggctgatcat catcgaccgc
     1801 gcgggcgtgg acaagctgac gccggaacag gatgcgcgct acaaccgcat cgccgacaac
     1861 gtgctgagcg ccacgcagga cgagctgatc gacacggtcg ccgccgcgca ggcgcccagc
     1921 ttcaccctgg ccgaaatcca tcaactgatc gacgccaacg cctcgcccgc cgccgcccgg
     1981 tacaacgcgg tcaagttcct gacgccggac gacaacgcca aggcggtgca gggctatatg
     2041 gtcgacgcgg tgatcaagat catcaaaagc tattcgcagt cgcagaccgg ctgacgcggc
     2101 ggcgcacggg gggattttca tgacgcttcg cttggttctt tacacagccc tggctgcggc
     2161 cctgatcgcc gccccggcgt cggcgcagac gcgccccatg ctgtccatcc tgtcgctgtc
     2221 cgggccggac gccaagcccg ccgacatcac cgcgggcctc gccgcgggcc agggcgcccc
     2281 gtccgcgccg agcgacgccg cgctcgacaa gcagaccgcc gaggcggtga cggcgctgta
     2341 cgagaaccgc accgccgatc aggccgcccg gcccgccgcc gacgccgtcg ccgatccggc
     2401 gctggccgag cgcatcgggc tggcgcgcgc cctgttccgt gtcgacggca ccgacgcgct
     2461 gatccggcat ttcgtcgaga ccgagcatat gaagctgatc atcgccgagg tggcgcggca
     2521 tatcgacttc tccaagctca gcgaaagcga caagtaccgg ctggccgcca tcgccgccaa
     2581 cgcgcaggcc gagctggagg acaggattct caatctcagc gcggcccaga ccgcgcaact
     2641 gatgaccaag ccggacctgc tgcaactgct ggccgccttc gatacggacg cgcagcgcaa
     2701 gctgaccgcc gtgcggctgg gcgacaacgg caagaccgac agcctcgccg aactcgacat
     2761 ccggctggcg cagtatcaga tcgtcaaaca gtacgagagc ggccagtaac gacgcccgca
     2821 gccttttccg actaccgcgc gttatccgcc accagccgcg ccgaggcgcc ttccagccgc
     2881 gccttgacct cggtcatgaa ttcggcgcgc ttcatcccgg cgggcagcgg cggcaggaac
     2941 tcgaacacca cggtgcccgg cttgcggtcg acgccgtggc cgggccagaa ctggccggaa
     3001 ttggtcgcca gcaggtgaca gggaacctgc aagtcgcggt acagcgccgc cacgcccggc
     3061 ttgtaatcgg gatcgtcgcc cggatcggag cgcgtgcctt cggggaagat cacgatctgc
     3121 cgcccctcgg cgaggctgcc ccggcaggcg gccaccatgc ttttcaacgc cttggccgcg
     3181 tcctcgcgct tgatcgccac catgcgcgtg cgccacgcgt accagccgaa gaagggcagg
     3241 tagatcagcg acttcttcaa cacataggcg ggcgccttca gcacgacgaa cggcgcgatg
     3301 gtgtcgagca tgctcatatg cttgcccgcg agcagcgccg cgccgtcggg ccggtgctcg
     3361 agaccgcgga actcgacgcg gatgccgacg atcaggcgcg cgccgaacag cacggccttg
     3421 gcccaccagc ggatgacggc cagtgcgcag accggcggcg ccagcagcag cggcgagaag
     3481 acgaggccga aaaacgccat ggagccatag agccacagca tgaacagcag ggagcggatg
     3541 cgcgacaaga taacctcacg aggcggaagc ggcggcctcg gagcccgatt tgggcgcttc
     3601 gcccttgtcg ccgaaggcgc gcgacacgct cagaatcgcg tcgcggccga ggatcgccag
     3661 atatttgcag tattccagca cgatcagcct ctggcccttg ggcgacttcc accagccgcg
     3721 cgcgtccagt tccggcgtcg ccaccggata ggcgatgaaa tgcgcgtcgg gcatgtcggc
     3781 cttcagctcc agcagggcgc gcggcatgtg atagtcggag gtgaccacga tcaggtcgta
     3841 gaagtcgtgc ccgcgcgccc agtcggcgat ctcgcgcgcg ttgccgacgg tgttttccgc
     3901 ctcgtagccg agatcgacgc agcattcgta gagccgccgg gagccttcgg tgacgtgcag
     3961 aagctgcggg cgcgtgacct cgcggttgac gccggagatg aacaggcgct ggcccttgcg
     4021 ccgctccagc aggcgcatcc cctccttcag gcgcagatcc gaggcgccgg tcagcaccac
     4081 gatggcgtcg gcgggcgcgt cgggttcgat ggccggggtg gaatcgatca cgcggtcggc
     4141 gaagaccagc agtccggcca gccagagcag cacgacgacg ccgagcgcaa tcaaggaccg
     4201 catgtgctta atgtccgcct ccccagatgc gtctaacgcc cgccgcccag caatctgagc
     4261 gtcaccatgc gcgccgtcac ggcggcgatc agcgccacca gcacgggaca ggggatcagt
     4321 attaacaggt cggaccacga aaagggaagg gccagcgcga agctttgtga ccggccggtg
     4381 acttttatca gcgccatcgc cacggcggcc agcccggcgc ccagaaggcc cgactcgaac
     4441 gccatgcgcg cgaagcggtt ctggaagcgt tcggcgatga agatgtccga ggcgccgcac
     4501 agcgacagca cctcgacgat attgccgcgc gccgccagcc ccgcgcgcgt ggcgaagccg
     4561 accacggcgg cggtcgccga accgatgatc aggaacacgc ccagcgacag gagccggatg
     4621 gtcagcgcgc tttgctgcac gtccttgagc cagaccgaat ggtcgtcgac cgaggcgtcg
     4681 atattctgcg ccgacagggc ctcgcggatg tcgtgcaggc tggccggatg gcgcggatcg
     4741 aggctgacct cgaccaggtt ggggatcggc aggtcgtcca gcaccgcgtc gcccagccac
     4801 ggcttgatca gatccttcgc cttttgcggc tccagcgccg tgacctgacc gacgcccttg
     4861 attccggcca gcgcctcggc ggcgtgagcg gcggcgaccg agccgctttc cagcccctgc
     4921 gggcgcacct gcacggtgat ttccgagcgg atgtcgcgcg accagcccgc cgccgcgcgg
     4981 tcggacgcca tcaccaccag cgcggccatg caggccagga agcacaggac gccgatgacg
     5041 taatgcagcg cgacctcgcg ttcgtcctga cgcggcaaga ggtcgcgcgc ctgtagattg
     5101 cggaagcggt cgaggaagaa gttgatcatc cgcccgcgcc tttcgcgagc gagcgccgcg
     5161 ccagccgccc gtccttcagc tccagcaccg gcatgcccga agcgcgcacc aggctttcgt
     5221 cgtgggtggc gatca
//
LOCUS       DASVLL010000531         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_520949,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000531 DASVLL010000000
VERSION     DASVLL010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520949"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VF402_08805"
     CDS             complement(<1..281)
                     /locus_tag="VF402_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800434.1"
                     /translation="MRLFIRTEASSAIGMGHFMRCFAIAEEARAQDMPVTFLLNALDD
                     ARGRLEAIGADGVVLPPLASGEDLLAMSGLGLSRRDWLVVDSYAADEAY"
     gene            complement(329..1330)
                     /locus_tag="VF402_08810"
     CDS             complement(329..1330)
                     /locus_tag="VF402_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEX7800435.1"
                     /translation="MTAWRTTGPVLRGVVTAMPETVSENADLAAGFGAETVAKIMAAT
                     GIERRPVSRTHTVSDLGAAAAARLLDGLGWDRSSVGLLIVVTQTPDYPLPSTACVLQN
                     RLGLAMGAAAFDLALGCSGYAYGLSVAASMMAAGGIERALIVAGDITTRMISEANRSL
                     APLFGDAAAATALELGGADIAFDLGSDGSGAPYLISKTGGLAEPGAPELFMDGTQVMA
                     FSLKQVAPSVARALAAAERTIDDMDYVVLHQANAMMLKTLAHKIRARDDQMVYAIRDY
                     GNTSSASIPLALSDHFGKAAPRAEVSLLLCGFGVGWSWSTAVWRTPWPAVHEIVRVS"
     gene            complement(1327..2070)
                     /locus_tag="VF402_08815"
     CDS             complement(1327..2070)
                     /locus_tag="VF402_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7800436.1"
                     /translation="MSTSGKWLILSGASRGLGLALARDLLAQGYGVAACSTTHNDDLA
                     ALEADHPARFLWRSVDMADAAAIDAFVAEAVRAPPGERLWGVINNAGVSVEGILSTLP
                     VRDIEHVLQVNLTGAICLARAALRRMMKARKSGRVINISSITGLRGYTGLSAYAASKA
                     GLDGFSRALAREVGRLGITVNAIAPGYMKTQLSEGLDDAQLAQIVRRTPLNRLCELAD
                     VVHLARFLLSEEAAFITGQTLVVDGGLTS"
     gene            complement(2067..>3101)
                     /locus_tag="VF402_08820"
     CDS             complement(2067..>3101)
                     /locus_tag="VF402_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX7800437.1"
                     /translation="LIGPEGDLAVAPAPGHGPHVVDVAQTGAAGLIMALLGAGDVYAV
                     RDPSAPPVVVAGEGRVHLQTSGTTGTPKWRAHEIANLTEGMAAGSTPARWLLTFHPGS
                     FAGVQVILSALIGGHALIAPPCDASVAEMADLAVRRQATHISGTPTFWRAFLMALGGR
                     TPDLRAVTLGGEAADQAILDALRARFPNAALRHIYATTEAGRVLTVGDGREGFPLAML
                     TGRLSLSEHGTLRVGGLDTHDAVEIAGDRVLFRGRLDTMVNIGGVKVFPETVEAFILR
                     HPGVQDVRVSPRPNPITGHMLTADMVLKPFADQNDVKAHLSGLPRAQRPVLTRFVESL
                     PTGQTGKKLR"
BASE COUNT          524 a         1118 c         1022 g          437 t
ORIGIN      
        1 atataggcct cgtccgccgc ataggaatcc accaccagcc agtcccgccg cgacaggccg
       61 agcccgctca tggccagtaa atcctcgccc gaagcgagcg gcggcaagac cacgccgtcg
      121 gcgccgatcg cctccagccg cccgcgcgca tcgtccagcg cgttgagcag aaacgtcacc
      181 ggcatgtcct gcgcccgcgc ttcttccgcg atggcgaaac agcgcatgaa gtggcccatg
      241 ccgatcgcgc tcgacgcctc ggtgcggatg aaaagcctca tggccaaaac cctgtcatgg
      301 cccaaaccct gtcatggccc aaaccctgtc atgaaacgcg cacgatctca tgcacggcgg
      361 gccagggcgt gcgccatacg gcggtggacc acgaccagcc gacgccgaag ccgcacagca
      421 acaggctcac ctcggcgcgc ggcgcggcct tgccgaaatg gtcgctgagc gccagcggga
      481 tcgaggccga ggaggtgttg ccgtagtcgc ggatcgcata gaccatctgg tcgtcgcgcg
      541 ccctgatctt gtgggccagc gtcttgagca tcatggcgtt ggcctgatgc agcacgacat
      601 agtccatgtc gtcgatggtc cgctccgccg ccgccagcgc gcgcgccacg gacggcgcga
      661 cctgcttgag cgaaaaggcc atgacctgcg tgccgtccat gaacagctcc ggcgcgcccg
      721 gttcggccag cccgccggtc ttcgagatca gatagggcgc gccggacccg tccgaaccga
      781 ggtcaaaggc gatgtcggcg ccgcccagtt ccagcgccgt cgccgccgcc gcgtcgccga
      841 acaaaggcgc gaggctgcgg ttcgcctccg aaatcatccg cgtggtgatg tcgcccgcca
      901 cgatcagcgc gcgttcgatc ccgcccgccg ccatcatcga cgcggcgacc gacaggccgt
      961 aggcgtagcc cgaacagccc agcgccagat cgaaggccgc cgcgcccatc gccagcccca
     1021 gccggttttg cagcacgcag gccgtcgacg gcagcggata gtccggcgtc tgggtgacga
     1081 cgatcagcag gccgaccgat gagcgatccc agccgaggcc gtccagcagg cgcgccgccg
     1141 ccgccgcgcc gaggtcgcta accgtgtgcg tcctcgacac cgggcggcgc tcgatgccgg
     1201 tggcggccat gatcttggcg accgtctccg cgccgaaacc ggcggccaga tcggcgtttt
     1261 cgctgaccgt ctccggcatg gccgtgacga ctccgcgtaa aacgggaccg gtcgtccgcc
     1321 acgccgtcat gaggtcagcc cgccgtcgac caccagggtc tggccggtga tgaacgccgc
     1381 ctcctcggac agcaggaagc gggccagatg cacgacgtcg gccagctcgc acagccggtt
     1441 cagcggcgtg cggcgcacga tctgcgccag ttgcgcatcg tccagccctt cggacagttg
     1501 cgtcttcata tagccgggcg cgatggcgtt gaccgtgatc cccagccgcc cgacctcgcg
     1561 cgccagcgcg cggctgaaac cgtcgaggcc cgccttcgac gccgcatagg cgctgaggcc
     1621 ggtatagccg cgcagaccgg tgatcgagga gatattgatg acgcggccgc tcttgcgcgc
     1681 cttcatcatc cggcgcaaag ccgcgcgcgc caggcagatc gccccggtca gattgacctg
     1741 caagacatgc tcgatatcgc gcaccggcag ggtggacagg atgccttcga ccgacacccc
     1801 ggcattgttg atcacgcccc acagccgttc ccccggcggc gccctcacgg cctccgccac
     1861 gaaggcgtcg atggcggcgg cgtcggccat atcgacggat cgccacaaaa agcgcgccgg
     1921 atggtcggcc tccagcgcgg ccagatcgtc attgtgcgtg gtcgaacagg cggcgacgcc
     1981 atagccctgc gccagcaggt cgcgcgccag cgccagcccc agcccgcgac tggcgccgct
     2041 caggatcagc catttgccgg aagtgctcat ctcagcttct ttcctgtctg gccggtgggc
     2101 aaggactcga cgaagcgcgt cagaacgggc cgctgcgcgc gcggcaggcc cgaaagatgc
     2161 gccttcacgt cattctgatc cgcaaaaggt ttcagaacca tatccgccgt cagcatatgg
     2221 ccggtgatcg ggttggggcg cggcgagacg cgaacgtcct gcacgcccgg atggcgcagg
     2281 ataaaggctt cgaccgtttc gggaaagacc ttgaccccgc cgatattgac catcgtatcc
     2341 agccgcccgc gaaacagcac gcggtcgccg gcgatctcga ccgcatcatg ggtgtcgagg
     2401 ccgccgaccc gcagggttcc gtgttcggaa aggctcagcc gtccggtgag catggccagc
     2461 ggaaagccct cgcgcccgtc gccgaccgtc agcacgcggc ccgcctcggt cgtggcgtag
     2521 atatggcgca gggcggcgtt cgggaagcgc gcgcgcaggg cgtcgaggat cgcctgatcc
     2581 gccgcttcgc cgcccagcgt cacggccctg aggtcgggcg tccggccgcc cagcgccatc
     2641 aggaaggcgc gccagaaggt cggcgtgccg gaaatgtgcg tcgcctgacg ccgcacggcc
     2701 agatcggcca tttcggccac gctggcgtca caaggcggcg cgatcagcgc atggccgccg
     2761 atcagggcgg ataaaatcac ctgcaccccg gcaaaactgc cgggatggaa ggtcaggagc
     2821 cagcgcgcgg gagtcgatcc ggcggccata ccttcggtca gattggcaat ctcgtgcgcg
     2881 cgccatttcg gcgttcccgt cgtgccggag gtttgcagat ggacgcgccc ctcgcccgca
     2941 acgacgacgg gcggcgcgga cggatcgcgc acggcataga cgtcgcccgc gcccagcagg
     3001 gccatgatca ggcccgccgc gccggtttgc gccacgtcga cgacgtgcgg gccgtgtccc
     3061 ggcgcaggcg cgaccgccag atcgccctcc ggtccgatca g
//
LOCUS       DASVLL010000532         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_357989,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000532 DASVLL010000000
VERSION     DASVLL010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357989"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VF402_08825"
     CDS             complement(<1..557)
                     /locus_tag="VF402_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX7800438.1"
                     /translation="MIALALHGGAGARAGRDYSLEIQHMAEVAAEARAALRVGAHALD
                     VVTDVVAALEASGLYVAGKGASPNAAGVYELDAAVMTGHDQRCGAVAALQGYASPVRV
                     ARAVMERTPHVLLAGDGAGLFAAGQGFEALSDPGAYFTRAGRFESNHPPGALAHGTVG
                     CVCRDAHGRLAAATSTAGVFGKLPGR"
     gene            complement(637..1263)
                     /locus_tag="VF402_08830"
     CDS             complement(637..1263)
                     /locus_tag="VF402_08830"
                     /inference="COORDINATES: protein motif:HMM:NF039735.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6348 family protein"
                     /protein_id="HEX7800439.1"
                     /translation="MSETLPDYRALQNDLSGLLRAERLTPQTRGDWVLVDDAFPALRA
                     HADQGRLHVELALYDGRIVRESFPDGGGPDDGWARFRDGPFRVFLSAFWERHNPGLVA
                     REVWKRGDGPWLALIGPYLRETDAGEAAPVPYPLFGVVRDFVRRQAMEDDLHWLSLHI
                     DVADGEAQVAARFDNAEQPELAEALRALDWPRDGRVHALRNFTLLARS"
     gene            1438..2148
                     /locus_tag="VF402_08835"
     CDS             1438..2148
                     /locus_tag="VF402_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEX7800440.1"
                     /translation="MSILMPKATAVWLVDNTSLSFEQIADFCGLHFLEVKGIADGEVA
                     RDIRGADPIANGQLTREELDKAEKTPSYRMKALVSRHAELLKPVKKGPRYTPVSRRQD
                     RPDAIKWFVKNHPEITDAQISKLLGTTKSTIEQVRNNTHWNSANIKPVDPVTLGLVSQ
                     IELDAVVRKAAEARRKHAEKMGIPLEDPALQPAQTVQPAFDEEEDSPYRKSDLSVEDV
                     FGAGSNTRRTDEDEDDAY"
BASE COUNT          377 a          757 c          721 g          303 t
ORIGIN      
        1 cggcccggca gcttgccgaa cacgcccgcc gtcgaggtgg cggcggccag ccgtccatgc
       61 gcgtcgcggc acacgcagcc gaccgtgccg tgcgccaacg cgcccggcgg atgattggac
      121 tcaaagcggc ccgcgcgggt gaaataggcg ccgggatcgc tgagcgcctc aaatccctgt
      181 ccggcggcga acaggccagc gccgtccccg gccagcagga cgtgcggcgt gcgctccatc
      241 accgcgcgcg ccacgcggac cgggctggcg tagccttgca gggcggcgac cgcgccgcac
      301 ctctggtcgt ggccggtcat caccgccgcg tcgagttcgt aaacgcccgc cgcgttgggc
      361 gaggcgccct tgcccgcgac atagaggccg gacgcctcca gcgcggcgac cacgtcggta
      421 acgacgtcga gcgcgtgcgc gccgacgcgc agggcggcgc gcgcctcggc ggcgacctcg
      481 gccatatgct ggatttcgag gctgtagtcg cgaccggccc tggcgccggc gccgccatgc
      541 agggccagcg cgatcatggg aaactccgtt gcatcggtaa cgtgtcttag gcgcgtgaac
      601 gccgccgcgc aagcctggct tctggttagc ttctggttag cttctggcca gaagggtgaa
      661 gttgcgcagg gcgtgaacgc ggccgtcgcg cggccagtcc agcgcgcgca aggcttcggc
      721 cagttccggc tgctcggcgt tgtcgaagcg cgcggcgacc tgcgcctccc cgtccgcgac
      781 gtcgatgtgc agcgacagcc agtgcagatc gtcctccatc gcctgccgcc gcacgaaatc
      841 ccgcacgacg ccgaacaggg gatagggcac gggcgcggcc tcgcccgcat cggtttcgcg
      901 cagataaggc ccgatcaggg ccagccacgg gccgtcgccg cgtttccaca cctcgcgcgc
      961 gacgaggccg ggattgtgcc gctcccagaa ggcgctgaga aagacgcgaa aagggccgtc
     1021 gcgaaagcgc gcccagccgt cgtccggccc cccgccgtcc gggaagctct cgcgcacgat
     1081 ccggccgtcg tacagagcca gctcgacatg cagccgcccc tgatcggcat gggcgcgcag
     1141 ggccggaaag gcgtcgtcga ccagcaccca gtcgccgcgc gtctgcggcg tcagccgctc
     1201 ggcgcgcaac aggccgctca ggtcgttttg cagggcgcga tagtcgggca gggtttcgga
     1261 catggtcgga gtcattaggg gacggccaag gttaaccgca atccggacct gcgtcgaatc
     1321 cgctcttttc tttcacgaaa aatcgcttta tagctgtcct caatccccgc gcccggctgc
     1381 aaaagccggg cgtagccatt tctggacgcc ggatgcgtcc tatcgagagg tgcgaacatg
     1441 tccatcctta tgcccaaggc cacggcggtc tggctggtcg acaacacatc gctgagcttc
     1501 gagcagatcg ccgatttctg cggcctgcac ttccttgagg tcaagggcat cgccgacggc
     1561 gaggtcgcgc gcgacattcg cggcgccgac ccgatcgcca acggccagtt gacgcgcgaa
     1621 gagctggaca aggcggaaaa gacgccgtcc taccgcatga aggcgctggt gtcgcgccac
     1681 gccgaactgt tgaagccggt caagaagggg ccgcgctaca ccccggtgtc gcgccgtcag
     1741 gaccgtcccg acgcgatcaa gtggttcgtc aagaaccacc ccgaaatcac cgacgcgcag
     1801 atttccaagc ttctgggcac caccaagtcg accatcgagc aggtgcgcaa caacacccac
     1861 tggaacagcg ccaacatcaa gccggtcgac ccggtgacgc tgggcctcgt ctcgcagatc
     1921 gagctggacg ccgtggtgcg caaggccgcc gaagcccggc gcaagcacgc cgaaaagatg
     1981 ggcatcccgc tggaagaccc ggcgctgcaa ccggcccaga ccgtgcagcc cgccttcgac
     2041 gaggaggagg acagccccta ccgcaagagc gacctgtcgg tcgaggacgt cttcggcgcc
     2101 ggttccaaca cccgccgcac ggacgaggac gaagacgacg cttattgagc ggtcggta
//
LOCUS       DASVLL010000533         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_197156,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000533 DASVLL010000000
VERSION     DASVLL010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197156"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..>1546
                     /locus_tag="VF402_08840"
     CDS             128..>1546
                     /locus_tag="VF402_08840"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7800441.1"
                     /translation="MRWMAAQRALVLGCALIGVAGAGHAQDLRADFMAPHDAARPLAR
                     WWWFGPAVRADELKREIAAMKAGGFGGFEIQPVYPLRLDGNLPYLSDDFLNMVRTADQ
                     TGRAAGLRVDVTLGSGWPYGGPQTGPDQASAHIRKLTQDAGLPLPVLKPGERFIAGFT
                     GPDAQSAQPVSLDRAIAARPGDKLFLFIQSPTGQQVKRAAVGAEGPVLDHMNRAAVEH
                     HLDTVADRLMTAFDTPPYSVFSDSLEVYGADWTGDLLAQFRKRRGYDLTPHLFDLFDD
                     SPATADVRHDWALTLNELVDERYLTTIEDWAKAHGTKFRAQVYGMPPVSLSSNRIPDL
                     PEGEGAHWRQFSTARWASSANHIYGRPVTSAESWTWVHTTPFRATPLDVKAEADVLML
                     EGINQFVAHGWPYSPPDAPEPGWSFYAAGVFNDHNPWWGVMPDLTGYLTRMSWLLRQG
                     GNVADVALYLPEDDALAATTPGK"
BASE COUNT          248 a          524 c          523 g          251 t
ORIGIN      
        1 cgcgttgcac tataggcgac cgttgcgttt cgaaactgca aaaatgtgtt cacatttgat
       61 cgaaaatgaa taatcatgtc gcaaaaggcg gggccttggg tacgccgccg gacgacagga
      121 gaggttcatg cggtggatgg cggcgcagcg cgcgctggtg ctgggttgcg cgcttatcgg
      181 cgttgcgggc gctggccatg cgcaggatct gcgtgcggat ttcatggcgc cgcacgacgc
      241 ggcgcggccg ctggcgcgct ggtggtggtt cggcccggcg gtgcgggcgg acgagctgaa
      301 gcgcgagatc gcggccatga aggcgggcgg tttcggcggc ttcgagattc agccggtcta
      361 tccgctgcgg ctcgacggca acctgcccta tctgtccgac gatttcctga acatggtcag
      421 gaccgccgac caaaccgggc gcgcggcggg tctgcgcgtc gacgtcacgc tcggctccgg
      481 ctggccctat ggcgggccgc agaccggccc cgatcaggcc tcggcccata tccgcaaact
      541 gacgcaggac gcgggcctgc ccctgcccgt cctgaaaccg ggcgagcgtt tcatcgccgg
      601 tttcaccggc cccgacgcgc aatcggcgca gccggtcagc ctcgaccggg cgatcgccgc
      661 gcggcccggc gacaagctgt tcctgttcat ccagagcccg acgggccagc aggtcaagcg
      721 cgccgccgtc ggcgccgagg gtccggtgct ggaccacatg aacagggccg ccgtcgaaca
      781 ccatctcgat acggtcgccg accggctgat gaccgccttc gatacgccgc cctattcggt
      841 gttcagcgac agtctcgaag tctatggcgc cgactggacc ggcgatctgc tggcgcagtt
      901 ccggaagcgg cgcggctatg acctcacgcc gcacctgttc gacctgttcg acgacagccc
      961 ggccaccgcg gacgtgcgcc acgactgggc gctgacgctc aacgaattgg tcgatgagcg
     1021 ctacctgacc accatcgagg actgggccaa ggcgcacggc acgaagttcc gcgcgcaggt
     1081 ttacggcatg ccgccggtca gcctgtcctc caaccgcatc cccgacctgc ccgaaggcga
     1141 aggcgcgcac tggcggcagt tctcgaccgc gcgctgggcc agttcggcca accatatcta
     1201 tggccgtccg gtcacctcgg cggaaagctg gacatgggtt cacaccacgc cgttccgcgc
     1261 cacgccgctg gacgtcaagg ccgaggccga cgtgctgatg ctggaaggga tcaaccagtt
     1321 cgtcgcccac ggctggccct attcgccgcc ggacgcgccg gagcccggct ggtccttcta
     1381 tgcggcgggc gtgttcaacg accataatcc gtggtggggc gtcatgcccg acctgaccgg
     1441 ctatctgacg cgcatgagct ggctgttgcg gcaaggcggc aacgtcgccg atgtggcgct
     1501 ttacctgccc gaggacgacg cgctggccgc gaccacgccg ggcaag
//
LOCUS       DASVLL010000534         7072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_411029,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000534 DASVLL010000000
VERSION     DASVLL010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411029"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1597)
                     /locus_tag="VF402_08845"
     CDS             complement(101..1597)
                     /locus_tag="VF402_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEX7800442.1"
                     /translation="MRTRISRRAFGASLTALAAASTAHAAPDGLPDWINPLRRDLEAM
                     AGRLTATLKPWSGPQRVFTPEAYGHVAGAALSTKAIQAAIDAAAQAGGGVARLAQGDY
                     VSGALDMRSGVKLDIAGGARLLGSLNLADWPERIARRRTVQDTNMGMNQSLIFAEGLR
                     DIAIGGDGTIDGRGGRFHGDETIHGTPGRPFLLRVIDCRNVHISGLFMKDSPCWMQNY
                     LNCDNLLIEKLRVENQCNWNNDGMDIDGCRNVIVRGCHISSGDDGICFKGAAQAPAEN
                     ILIENCTVFTSCNALKLGTDSQSGFRNVLARNLILGGVDDTMRHIKPVDASTGISWEM
                     VDGGHAENILCRNIEIVRARSPFFLRLDDRGRVQPDQPKPPVGSLRRIVFEHVRGADN
                     GPRGSFFIGAPSQAVEDVALIDVRLSQRPAAKAVAEADIPEMHDDYPDADMLRPHGDL
                     APAYGLWARHVHGLTLKEYRVTPTGPDPRPEYVMDSDVSGAIDGISHV"
     gene            complement(1719..2246)
                     /locus_tag="VF402_08850"
     CDS             complement(1719..2246)
                     /locus_tag="VF402_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7800443.1"
                     /translation="MIETILIVDDNADLAATLARSLTRRGYRAHVEASTEAALAVTAM
                     VRPQAAVVDLRLGGASGLECVKGLHAFDPAMRIVVLTGYASITTAVEAVKLGACHYLA
                     KPANAEDILAAFGRAEGETGVEIAAAPTSLKNLEWERINEVLIETDYNISETARRLGL
                     HRRTLARKLAKRPVR"
     gene            complement(2243..3556)
                     /locus_tag="VF402_08855"
     CDS             complement(2243..3556)
                     /locus_tag="VF402_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7800444.1"
                     /translation="MTSYQSIEDAANRKNLRVLIALRGMAFCVQAGLIAYAAYGLGIV
                     LPLPLMLATASVLIAFNLWSVWRLRQARPVHDLDLFAGLAIDVIVLAAQLDVSGATSN
                     PFVSVFMLPVILGAVMLAPARAWLIYGLTLICYAALAVSAAHRATPMLMDMGGGAPAG
                     RGAALVDMSALHINGMMFGYAVCAGALVFLIARIRGNLRARDQEVADLRARGLEQAHL
                     MRLGLLTAGAAHELGTPLTTLSVILKDWQDFGPPDDDAREAEIAAMRDQVARCKTIIS
                     DILTASGRTRGEGAQAVALDAFVQAAARSWRDRHPATPLDADIRLGGAVIAGDRVLEQ
                     ALHNLLDNAAEASPSGVRLSGRLDGGDLILRVEDDGAGFAPDILAGLGQPYLTTKTGD
                     APRGLGLFLAVNTAQALGGRLDARNMARGAEVALIVPMRALSMTA"
     gene            3645..5843
                     /locus_tag="VF402_08860"
     CDS             3645..5843
                     /locus_tag="VF402_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800445.1"
                     /translation="MTPLRPLPLLLAAALPLCAHAETATEVVVRGQRKAPDVPTTSEG
                     VTAEALARAVNVVTPEDALRYAPNVLIRQRHIGDTQSPVSTRTSGVGGSARSLIYVDG
                     VLISALIGNNNTSASPKWGLIAPDAIARVDVLYGPYAAAYAGNSLGGVILFETRMPTK
                     LEGGITVQGAVQDFRKYGDKDRYGVGRVAADIGDRNGRLAWRLSLNRLDARSQPLSYV
                     TANALPSGAGGGFGALNRSGAPVRILGAGGLEHQTQDNVSGRVVYDLTPALTAAYTFG
                     LFVNDDRAHAHSYLSDSGGAPVYTSGFTSGVYRLRETQLAQGLSLTSHTGGAFDYSLT
                     ASVFAYLNSHQRAPTGLLPAAAAGGPGTDAALDGTGWNTLDAQGTWRPGGAHVVSFGL
                     HQDAFRLDNPKYKVADWRGGDEGALIADSRGRTRTQAAWAQDAMTLGPRTRLTVGLRL
                     EHWRASGGENYSAAPALDVRQPALTRDAASPKLVLAFNPTPDWTLKASLGAAARFPTV
                     TELYQAVTTGPVLSTPAPDLRPEQAVSSELSAQKTWPDASLRVSLFHESIRDALLSQS
                     APLAAGSSTLYTYVQNVDRTRGGGIEIVGDRSDFLIRNLQISGWATYAEVKVDKDTAF
                     AAAVGKDIPQLPRLRASLVLTYAPAPRWDVTLAARYSGPAYATIDNSDRVHDVYTGFD
                     GFTVIDLHVRYRLDAHWSAEAGIDNLNDAAYFIYHPFPQRTALLTLKYRL"
     gene            6175..>7072
                     /locus_tag="VF402_08865"
     CDS             6175..>7072
                     /locus_tag="VF402_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7800446.1"
                     /translation="MLALTLGLAASATAQTSGTSAPHDDTGGNSTVVVVTGYRASLQS
                     SLNAKKHADVMLDAITADDIASFPEANLAESLQRIPGISIDRDNGEGRTISVRGLGGD
                     FTRVRINGMEALSTAGANDSGSSPNSSRAFDFNTFASELFSRVTVRKSASAETDEGSL
                     GATVDLTTGHPFDYKGQKFALSAEDAYYENGKHQNPRLAGLYSNRWFGGRLGFLVSAA
                     YQQRDTSISSYARSAGSADYTYRGTTWATLPAKGAPKVYGFAAPTGTNLGAYGITNPT
                     AIAALTGSDPDAYAALYPDGFGS"
BASE COUNT         1122 a         2454 c         2381 g         1115 t
ORIGIN      
        1 gaggtaatag cgccggtcca cccggatcac gtccgggtcg gaatagtcgg cgtagagaac
       61 gggattggcg taggtcagcg cggcggcggc gatcagggcg ctaaacatgg ctgatcccgt
      121 caatggctcc gctgacgtcg ctgtccatca catattccgg gcgcggatcg ggaccggtgg
      181 gcgtgacgcg atactccttc agcgtcagcc catgcacatg ccgcgcccac agaccgtagg
      241 ccggggcgag gtcgccgtgc ggccgcagca tgtcggcgtc gggatagtcg tcgtgcattt
      301 cgggaatatc ggcctcggcg accgccttgg ccgccgggcg ctgcgacagg cgcacgtcga
      361 tcagcgccac gtcctcgacg gcctgcgacg gcgcgccgat gaagaacgag ccgcgcgggc
      421 cgttgtcggc gccgcgcaca tgctcgaaca cgatgcggcg caggcttccg accggcggtt
      481 tcggctggtc cggctgcacc cgtccgcgat cgtcgaggcg caggaagaac gggctgcgcg
      541 cgcgcacgat ctcgatgttc cggcacagga tattctcggc atggccgccg tcgaccatct
      601 cccacgatat gcccgtcgag gcgtccacgg gcttgatatg ccgcatcgtg tcgtccaccc
      661 cgccgaggat caggttgcgc gccagcacgt tgcggaatcc gctctgcgaa tccgtgccga
      721 gcttgagcgc gttgcacgag gtgaacacgg tgcagttctc gatcaggatg ttttccgccg
      781 gggcctgcgc cgcgcccttg aagcagatcc cgtcgtcgcc ggagctgata tggcagccgc
      841 gcacgatgac gttgcggcag ccgtcgatat ccatgccgtc attgttccag ttgcactggt
      901 tctcgacgcg cagcttctcg atcagcaggt tgtcgcagtt gagatagttc tgcatccagc
      961 acggcgaatc cttcatgaac aggccgctga tatgcacgtt gcggcaatcg atgacgcgca
     1021 gcaggaacgg tcggcccggc gtgccgtgga tcgtctcgtc gccgtggaag cgtccgccgc
     1081 gcccgtcgat cgtcccgtcg ccgccgatgg cgatgtcgcg caggccctcg gcgaagatca
     1141 gcgactggtt catgcccata ttggtgtcct gcaccgtgcg ccgcctggcg atgcgttcgg
     1201 gccagtccgc aagattcagg ctgcccagaa ggcgcgcgcc gcctgcgatg tcgagcttca
     1261 cgccgctgcg catatcgagc gcgccgctga cgtaatcgcc ctgcgccagc cgcgccacgc
     1321 cgccgcccgc ctgcgccgcc gcgtcgatgg cggcctgaat cgccttggtg gacagggccg
     1381 cgcccgccac gtggccgtaa gcctccggcg tgaagacgcg ctgcggcccg gaccacggtt
     1441 tgagcgtggc ggtcagccgc ccggccatcg cctccagatc gcgccgcagc gggttgatcc
     1501 agtcgggcag accatcgggc gccgcatggg ctgtggaggc ggcggcgagg gcggtgaggc
     1561 tggcgccgaa ggcgcgtcgt gatatccggg ttcgcatttt cctgcctgtc tttttccgtc
     1621 agtgtgccgc atgggccgcg aaaaggaaag aaagcccggc aggggtgaac cgccgggcct
     1681 gtcagttgca agacctcacg gggagcgagg ccggcgcctt atcgcaccgg gcgcttggcc
     1741 agtttgcgcg ccagggtgcg gcggtgcagg cccagacggc gggcggtttc ggagatgttg
     1801 taatccgtct cgatcagcac ctcgttgatg cgttcccatt cgaggttttt cagcgaggtc
     1861 ggcgcggcgg cgatctcgac gccggtttcg ccttccgcgc gcccgaaggc ggcgaggatg
     1921 tcctcggcgt tggcgggctt ggcgaggtag tggcaggcgc ccagcttgac cgcctcgacc
     1981 gccgtggtga tgctggcgta gccggtcagc acgacgatgc gcatcgccgg atcgaaggcg
     2041 tgcaggccct tcacgcattc cagccccgac gcgccgccta gcctgagatc gaccacggcg
     2101 gcctgcgggc gcaccatcgc cgtgacggcc agcgccgcct cggtcgaagc ctcgacatgc
     2161 gccctgtagc cgcgccgcgt cagcgaccgc gccagcgtgg cggcgaggtc ggcgttgtcg
     2221 tcgacgatca ggatggtctc gatcatgcgg tcatgctcag cgcgcgcatc ggcacgatca
     2281 gcgcgacttc ggcgccgcgc gccatattgc gcgcgtccag ccgtccgccc agcgcctgcg
     2341 ccgtgttgac ggccaggaac aggcccagcc cgcgcggcgc gtctccggtc ttggtggtga
     2401 gatagggctg gcccagcccg gccaggatgt cgggcgcgaa gcccgcgccg tcgtcctcga
     2461 cgcgcaggat caggtcgccg ccgtcgaggc ggccgctcag gcgcacgccg gacggcgacg
     2521 cctcggcggc gttgtcgagc aggttgtgca gggcctgctc cagcacgcgg tcgcccgcga
     2581 tgacggcgcc gccgagccgg atgtcggcgt cgagcggcgt cgcgggatgg cggtcgcgcc
     2641 agcttcgggc ggcggcctgc acgaaggcgt ccagcgcgac ggcctgcgcg ccttcgccgc
     2701 gcgtccggcc cgacgcggtc aggatgtcgg agatgatcgt cttgcagcgc gcgacctgat
     2761 cgcgcatggc ggcgatctcg gcctcgcgcg cgtcgtcgtc gggcgggccg aaatcctgcc
     2821 agtccttgag gatcaccgac agggtggtca gcggcgtgcc cagttcgtgc gccgcgcccg
     2881 ccgtcagcag gcccagccgc atcaggtgcg cctgctccag cccgcgcgcg cgcagatccg
     2941 ccacctcctg atcgcgcgcg cgcagattgc cccggatgcg cgcgatcagg aagaccagcg
     3001 ccccggcgca gaccgcatag ccgaacatca tgccgttgat gtgcagggcc gacatgtcga
     3061 ccagcgccgc gccgcgtccg gcgggcgcgc cgccgcccat gtccatcagc atcggcgtgg
     3121 cccgatgcgc ggcggagacg gccagcgccg cgtaacagat cagggtcagg ccgtagatca
     3181 gccaggcgcg cgccggcgcc agcatgacgg cgcccagaat gaccggcagc atgaagaccg
     3241 acacgaacgg gttggaggtc gcgccgctga cgtcgagctg cgcggccagc acgatcacgt
     3301 cgatcgccag tcccgcgaac aggtcgagat catgcacggg ccgggcctga cgcaggcgcc
     3361 agaccgacca gagattgaac gcgatcagta ccgaggcggt cgccagcatg aggggcaggg
     3421 gcaaaacgat gccgaggccg tacgccgcat aggcgatcag ccccgcctgc acgcagaacg
     3481 ccatgccgcg cagcgcgatc agcacgcgca ggttcttgcg gttggccgcg tcttcgatgc
     3541 tttgatagga ggtcatgcga ggtcttttat aacgcctcct tcatgcctgc gcttggacgt
     3601 tttgtccaat aggcgtcggc gcgcggcggc gatacagccc ggccatgacc ccgttgcgcc
     3661 ccctgccgct gcttctcgcc gccgccctgc cgctctgcgc ccacgccgaa accgccaccg
     3721 aagtcgtggt caggggccag cgcaaggcgc ccgacgtccc gaccaccagc gagggcgtca
     3781 ccgccgaggc gctggccagg gcggtcaatg tcgtcacgcc ggaggacgcg ctgcgctacg
     3841 cgccgaacgt gctgatccgc cagcgccata tcggcgacac gcaatcgccg gtcagcacgc
     3901 gcacctccgg cgtcggcggt tcggcgcgca gcctgatcta tgtcgacggc gtgctgatct
     3961 cggcgctgat cggcaacaac aacacctcgg cctcgccgaa atgggggctg atcgcgccgg
     4021 acgcgatcgc gcgcgtcgac gtgctgtacg ggccttacgc cgccgcctat gcgggcaaca
     4081 gcctcggcgg cgtcatcctg ttcgagacgc gcatgccgac gaagctggaa ggcgggatca
     4141 cggttcaggg cgccgtgcag gatttccgca aatacggcga caaggaccgt tacggcgtgg
     4201 gccgcgtcgc cgccgatatc ggcgaccgca acgggcggct ggcctggcgg ctcagcctca
     4261 accgtctcga cgcccgctcg cagccgctga gctatgtgac cgcgaacgcc ctcccgtccg
     4321 gcgccggcgg cggattcggc gccctcaacc gctccggcgc gccggtccgg attctgggcg
     4381 ccggcgggct ggagcatcag acgcaggaca atgtgtcggg ccgcgtcgtt tacgacctga
     4441 cgcccgcgct gacggcggcc tatacgttcg gcctgttcgt caatgacgac cgggcgcacg
     4501 cgcacagcta cctgagcgat tccggcggcg cgccggtcta tacgtccggc tttaccagcg
     4561 gggtttaccg tctgcgcgag acgcaactgg cgcagggcct gtccctgacc tcgcacacgg
     4621 gcggcgcgtt cgattacagc ctgaccgcct ccgtgttcgc ttacctgaac agccatcagc
     4681 gcgcgccgac cggcctcttg cccgccgcgg cggcgggcgg gccggggacc gacgcggcgc
     4741 tggacggcac gggctggaat acgctcgacg cgcagggaac ctggcgtccc ggcggcgcgc
     4801 atgtcgtcag tttcggcctg catcaggacg ccttcaggct cgacaacccg aaatacaagg
     4861 tcgccgactg gcgcggcggc gacgagggcg cgctgatcgc cgacagccgg ggccgcaccc
     4921 gcacgcaggc ggcgtgggcg caggacgcga tgacgctggg gccgcgcacg cgcctgaccg
     4981 tcgggctgcg gctggaacac tggcgcgcca gcggcggcga aaactattcc gccgcgcccg
     5041 cgctcgacgt gaggcaacct gcgttgacgc gcgacgccgc ttcgccgaag ctggttctgg
     5101 ccttcaatcc gacaccggac tggacgctga aagcctcgct gggcgccgcc gcgcgctttc
     5161 cgaccgtcac cgagctttat caggccgtga ccaccggccc ggtgctgagc acgcccgcgc
     5221 cggacctgcg ccccgaacag gccgtgtcgt cggagctgtc ggcgcagaag acctggccgg
     5281 acgcttcctt gcgcgtctcg ctgttccacg agtcgatcag ggacgcgctg ctgtcgcaga
     5341 gcgcgccgct ggcggcggga tcgtccacgc tctacaccta cgtccagaac gtcgaccgca
     5401 cgcgcggcgg cgggatcgag atcgtcggcg acaggagcga tttcctgatc cgcaacctgc
     5461 agatttccgg ctgggcgacc tacgccgagg tgaaggtgga caaggatacg gccttcgccg
     5521 ccgccgtggg caaggacatt ccgcaactgc cgcgcctgcg cgcatcgctg gtcctgacct
     5581 atgcgccggc gccgaggtgg gacgtgacgc tggcggcgcg ctattcgggt ccggcctacg
     5641 ccaccatcga caacagcgac cgcgtccatg acgtctatac cggcttcgac ggcttcaccg
     5701 tcatcgacct gcatgtgcgc taccggctcg acgcgcactg gagcgccgag gccgggatcg
     5761 ataacctgaa cgacgcggcc tatttcatct atcacccgtt cccgcagcgc acggcgctgc
     5821 tgacgctgaa ataccggctg taaagccgtg gcgctttgat cgtacaataa agcaaatata
     5881 ttcaaagtcc gggcgcggcg gcggccttgc tttgtgaaaa gcgtctcaca tataaaatgg
     5941 ccatgacagc gctgacatga aaaaaccgta ttgacgccgg ttttatttcc gccaatatga
     6001 caccggtttc catttatggt ccgacaattt cggcccaaat ccaagactcg aatctcaggc
     6061 acagcacgac ccgccggtca cataaaaaat tccctaaggc cggtggatac aggaaggacg
     6121 acacacgtga cccaacagac atcccaggcg aagtctcttt atttttgcgc atcggtgtta
     6181 gcgttaacgc tcggcctcgc ggcgtcggcc acggcccaga cgtcggggac atccgcaccg
     6241 catgatgaca ccggtggcaa ctcgaccgtc gtcgtggtca ccggttatcg cgcctcgctg
     6301 caatcgtcgc tgaacgccaa gaagcacgcc gacgtcatgc tggacgcgat cacggcggac
     6361 gacatcgcct cgttcccgga agcgaacctg gccgaatcgc tgcaacgcat tccgggcatc
     6421 tcgatcgacc gcgacaacgg cgagggccgg accatttcgg tgcgcggcct cggcggcgac
     6481 ttcacccgcg tgcgcatcaa cggcatggag gccctgtcga cggcgggcgc caacgattcg
     6541 ggctccagcc ccaactcgtc gcgcgccttc gatttcaaca ccttcgcctc ggagctgttc
     6601 agccgcgtga ccgtgcgcaa gagcgcctcg gcggagaccg acgaaggctc gctgggggcc
     6661 acggtcgacc tgaccaccgg ccatccgttc gactacaagg gccagaagtt cgccctgtcg
     6721 gcggaggacg cctattacga gaacggcaag catcagaatc cgcgcctcgc gggcctgtac
     6781 tcgaaccgct ggttcggcgg caggctcggc ttcctcgtct ccgccgccta tcagcagcgc
     6841 gacacctcga tcagctccta cgcgcgctcg gccggttcgg ccgactacac ctatcgcggc
     6901 acgacttggg cgactttgcc cgccaagggc gcgccgaagg tttacggctt cgccgcgccg
     6961 accggcacga atctgggcgc ttacgggatc accaatccga cggcgatcgc ggccctgacc
     7021 ggctcggacc cggacgccta tgcggcgctt tatcccgacg gtttcggctc gg
//
LOCUS       DASVLL010000535         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_411068,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000535 DASVLL010000000
VERSION     DASVLL010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411068"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /locus_tag="VF402_08870"
     CDS             complement(<1..1896)
                     /locus_tag="VF402_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7800447.1"
                     /translation="MAQRPVFFDPTGRRKRASFIGAVALIAAALVLFVGLVLSILVVP
                     VPEGLDLHMEYPRPEASKIVLNHANPRHRPLTASRGDSNSNVNQTVAAFYTPWDDASR
                     ASLEKHINNIDILAPVLASVTGKDHKFLYQRDDNLTYLLQNASHRPAVLIVVQNVAQD
                     NWDGPDTARLLNNAQARRKLLGQIGDMLRAEKAQGVTFDFEALPDFSLTAYLNFIREA
                     KAQLGPQGFAVTLTAPVDTTVWNLRAFAGAADRLFLMIYDQHSMADPPGPIAAQGWFN
                     ERLAYALKSVPRDKAIVAIGSYAYDWTNMPGGGINVTPRTVEEAWRIARESQAQIVFD
                     PVSGNAHFSYSQNGVDHEVWLLDAVSAWNQLRSIDDSNVWGVALWRIGSEDPGVWADL
                     RAFQTKATPDLSKLTPLGDVDVEGNGEIVHIDATPTTGSRDVVFDRQRIAQDETYKEL
                     PTPYVVRRTGYAPGKLALTFDDGPDARWTPRILDILKREHVPATFFVIGEKAIEHPEL
                     LRRMVAEGHTLGSHSYTHPDMSKVPPSWLRFELNSTQRVVEAYTGRSLRLLRIPYFGD
                     AEPTTENELGPALAAQRLGYVNVGLHVDAEDWQRPGVPAIIHNVLQGVADGEASQLTP
                     QCKYDASE"
     gene            complement(1987..>4051)
                     /locus_tag="VF402_08875"
     CDS             complement(1987..>4051)
                     /locus_tag="VF402_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800448.1"
                     /translation="HKADRDVYDVTQDPDAATGSAADVMNNIPAVTVDQDGAVALRGD
                     QNVQIYINGKPSPQMQGDNRGPALQSLSADDIDSVEVMTNPSAAFGADTGGGIINIVL
                     KKGRHVKPRTSLNVAAGDAGRYQFGFRSGTNIGKLTLNGNVNLRSDARKNGGHSDRAR
                     IDPDTGATRDSTQDNQSNTRTRDLSANLNADYDLSDNDTLSAELNYARHDRDGYNVGE
                     YRYDAAAGSPASQTAQAGTTRGVSSDGGVRLTFDHRGDTPDNDFKMQLAHSQSRPDNA
                     SDYRQIYHQPQKPDSLYATRNRTQNVNDDFSGDWKRAFDGGHQVQAGWDVRHTQSDAY
                     NYRSMNHAEGAPEAANPNYTNQFNVDQTISAAYVTYQRTLGRFSAQVGIRGEKLHENL
                     DQVTSGLSAVVDQVNWSPSLFALYSLNDDDRARLSYGQKIVRPGAWLLNPFLRYGDAQ
                     NVSSGNPYLQPERIETWELEYDHTTRPLNYSATLFYKTARDNFSRASSYLPGAGDVLL
                     TTNINDGSRRDTGLQYALSGQNDVLRYGLNGVLGWSAQDATDYVTHAPVHRESGNSSA
                     RIYARWSPDKRNTFGVNLRWRGRQLLSQGYRTGSTSLNLSYQYQIVPNKAQITFNARD
                     VLSGEGTRSVTRTSTVYNVSERYDYGATFMMALRYTFGPAPKTPPPPEPGRPKWRGYG
                     GGSGL"
BASE COUNT          586 a         1285 c         1418 g          762 t
ORIGIN      
        1 ttccgacgcg tcgtacttgc attgcggcgt caactggctg gcctcgccgt cggcgacgcc
       61 ttgcagcacg ttgtggatga tggcgggaac ccccggcctt tgccagtcct cggcgtcgac
      121 gtgcagcccg acattgacgt aacccagccg ctgcgccgcc agcgcgggcc ccagttcatt
      181 ctcggtcgtc ggctcggcgt cgccgaaata gggaatgcgc agcagccgca gcgagcggcc
      241 cgtataggcc tcgacgacgc gctgggtcga gttcagctcg aagcgcagcc acgagggcgg
      301 caccttggac atgtccgggt gggtgtagga atggctgccc agcgtgtggc cctcggcgac
      361 catgcggcgc agaagttcag gatgctcgat ggccttttcg ccgatgacga agaaggtcgc
      421 cgggacatgc tcgcgcttga ggatgtcgag gatgcgcggc gtccagcgcg cgtccggccc
      481 gtcgtcgaag gtcagggcca gcttgcccgg cgcgtagccg gtgcggcgca cgacataggg
      541 cgtcggcagt tccttgtacg tctcgtcctg cgcgatgcgc tgacggtcga acacgacgtc
      601 gcggctgccc gtggtcggcg tggcgtcgat atgcacgatc tcgccattgc cctcgacgtc
      661 gacatcgccc agcggggtca gcttcgacag gtcgggcgtg gccttggtct ggaacgcgcg
      721 caggtccgcc cagacgccgg ggtcttcgga gccgatgcgc cacagcgcga cgccccagac
      781 gttcgaatcg tcgatggagc gcaactggtt ccacgcgctg accgcgtcca gcagccagac
      841 ctcgtgatcc acgccgttct ggctgtagga gaagtgcgcg ttgcccgaca ccgggtcgaa
      901 cacgatctgc gcctgcgatt cgcgcgcgat gcgccacgct tcctcgaccg tgcgcggcgt
      961 gacgttgatc ccgccgccgg gcatgttcgt ccagtcgtag gcgtaggagc cgatggcgac
     1021 gatcgccttg tcgcgcggca cggatttgag cgcataggcc agccgctcgt tgaaccagcc
     1081 ctgcgccgcg atcggccccg gcggatcggc catcgaatgc tggtcgtaga tcatcaggaa
     1141 caggcggtcg gccgcgcccg cgaaggcgcg caggttccac accgtcgtgt cgaccggcgc
     1201 ggtcagggtg acggcgaagc cctgcggccc cagttgcgcc ttcgcctcgc ggatgaagtt
     1261 gagataggcg gtcaggctga aatcgggcag cgcctcgaag tcgaacgtca cgccctgcgc
     1321 cttttcggcg cgcagcatgt cgccgatctg gcccagcagc ttgcgccgcg cctgcgcgtt
     1381 gttcagcagc cgcgccgtgt ccggcccgtc ccagttgtcc tgcgcgacgt tctgcaccac
     1441 gatcagcacc gccggcctgt gcgaggcgtt ctgcaacagg taggtcaggt tgtcgtcgcg
     1501 ctgatagagg aatttgtggt ccttgccggt gaccgaggcc agcaccggcg ccaggatgtc
     1561 gatattgttg atgtgttttt ccagcgaggc gcggctggcg tcgtcccacg gcgtatagaa
     1621 ggcggcgacg gtctggttga cattggagtt ggagtcgccg cggctggcgg tcagcgggcg
     1681 gtggcgcgga ttggcgtggt tcagcacgat cttggaggct tccgggcgcg gatactccat
     1741 gtgcaggtcc agcccctccg gcaccggcac gaccaggatg gacagcacca gcccgacgaa
     1801 cagcaccagc gccgccgcga tcagggcgac ggcgccgatg aaggaggcgc gcttgcggcg
     1861 ccctgtggga tcaaaaaaaa ccggtctttg cgccatgccc gctcacgatg cgaataatcc
     1921 gccgcttatt cgcgccgatt atgtcaaatc gcagacgatt cgtaagccgt cgacaaaaat
     1981 ctgtcctcag aggccgcttc cgccgccata gccgcgccat ttcggccgtc ccggctccgg
     2041 cggcggcggc gttttcggcg ccgggccgaa ggtgtagcgc agcgccatca tgaaggttgc
     2101 gccgtaatcg tagcgctcgg agacgttata caccgtgctg gtgcgggtca cgctgcgcgt
     2161 cccctcgccg gacagcacgt cgcgtgcgtt gaaggtgatc tgcgccttgt tcggcacgat
     2221 ctgatactga tacgacaggt tcagcgaggt cgagccggtg cggtagccct gcgacagcaa
     2281 ctgccgcccg cgccagcgca ggttgacgcc gaaggtgttg cgtttgtccg gcgaccagcg
     2341 cgcatagatc cgggcgctgg aattgccgct ttcgcgatgc accggcgcgt gggtgacgta
     2401 gtccgtcgcg tcctgcgccg accagcccag cacgccgttc agcccgtagc gcagcacgtc
     2461 gttctggccg ctcagcgcgt attgcaggcc cgtgtcgcgg cgcgagccgt cattgatatt
     2521 ggtggtcaaa agcacgtcgc ccgcgccggg caggtaggag ctggcgcgcg agaaattatc
     2581 gcgcgccgtc ttgtagaaca gcgtcgccga atagttgagc ggccgcgtcg tgtggtcgta
     2641 ttccagctcc caggtctcga tccgctccgg ctggaggtac ggattgccgg aactgacgtt
     2701 ctgcgcgtcg ccgtagcgca ggaacgggtt gagcagccag gcgccgggcc gcacgatctt
     2761 ctggccgtag ctgaggcgcg cgcggtcgtc gtcgttcagg ctgtagagcg cgaacaggct
     2821 ggggctccag ttgacctgat cgaccacggc gctcaggccg gacgtgacct gatcgaggtt
     2881 ttcgtgcagc ttctcgccgc gaatcccgac ctgcgcgctg aaccggccca gcgtgcgctg
     2941 ataggtgaca taggcggcgg agatcgtctg atcgacgttg aactggttgg tgtagttggg
     3001 gttggcggct tccggcgcgc cctcggcgtg gttcatcgag cggtaattat aggcgtccga
     3061 ctgcgtgtgc cgcacgtccc agcccgcctg cacctgatgg ccgccgtcga aggcgcgctt
     3121 ccagtcgccg ctgaaatcgt cgttgacgtt ctgcgtgcgg ttgcgcgtgg cgtagagcga
     3181 atccggcttt tgcggctggt gatagatctg gcggtagtcg ctggcgttgt ccgggcgcga
     3241 ttgcgagtgc gccaactgca tcttgaagtc gttgtcgggc gtgtcgccgc gatggtcgaa
     3301 ggtcaggcgc acgccgccgt cgctggagac gccgcgcgtg gttcccgcct gcgcggtctg
     3361 cgaggccggg ctgccggcgg cggcgtcata gcggtattca ccgacattat agccgtcgcg
     3421 gtcgtggcgg gcgtagttca gctccgccga caaggtgtcg ttgtcggaca ggtcgtaatc
     3481 ggcgttgaga ttggccgaaa ggtcgcgggt gcgcgtgttc gactggttgt cctgcgtgga
     3541 atcgcgcgtc gcgccggtgt cgggatcgat gcgcgcgcgg tcgctgtggc cgccgttctt
     3601 gcgcgcgtcc gaacgcaggt tgacgttgcc gttcagggtc agcttgccga tattggtccc
     3661 ggagcggaag ccgaactgat agcggcccgc gtcgcccgcc gcgacgttga gcgaggtgcg
     3721 cggcttcacg tgccgcccct tcttcagcac gatgttgatg atcccgccgc ccgtatcggc
     3781 gccgaaggcc gccgacgggt tggtcatgac ctcgaccgag tcgatatcgt ccgccgacag
     3841 gctttgcagc gccgggccgc ggttgtcgcc ctgcatctgc gggctgggct tgccgttgat
     3901 gtagatctgc acgttctggt cgccgcgcag cgcgacggcg ccgtcctgat cgaccgtcac
     3961 cgccgggatg ttgttcatca cgtcggcggc gctgcccgtg gccgcgtccg ggtcttgcgt
     4021 gacgtcatag acgtcgcggt cggccttgtg c
//
LOCUS       DASVLL010000536         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_197483,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000536 DASVLL010000000
VERSION     DASVLL010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197483"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF402_08880"
     CDS             <1..492
                     /locus_tag="VF402_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800449.1"
                     /translation="LGLIWFPKNLPNVLNGLGFQGSVTTLASSQNVPDTDAAGNIVAQ
                     LKTPFFGVSRYSYNATLAYDKGPIGARLSYVWRSGFLASNEASLFANPLGIWRHPEKS
                     LDFQLTYNINDRMSFDIDAVNLTNEMQQQYYHFGDAGSPELTDFGTVQIGRSVSVGFR
                     WKM"
     gene            620..1504
                     /locus_tag="VF402_08885"
     CDS             620..1504
                     /locus_tag="VF402_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7800450.1"
                     /translation="MYRRLMTLEQLRIFVAVAEREHLTRAAESLCLTQSAVSAAVAQL
                     EARFGVKLFDRIGRGIALTEAGRLFLPEARAVLARAHEAEALVRDVAGALRGSLRICA
                     SQTVGNFWLPARLARFQAAFPGLVLHVEHLNTERAAAKVLAHEADIAVVEGGIGDPRL
                     SATALSGDRMLLVLPNGWAAPRAPEDWAALTFVTREPGSGTRALLADHLNGMGLRLDA
                     RNIALELPSNEAVRGAVEAGAGASLLSELVTQRALRTGDVQGIDIGLPPRPFYALRLK
                     ERAGTRAESEFIRALCAG"
     gene            complement(1482..2006)
                     /locus_tag="VF402_08890"
     CDS             complement(1482..2006)
                     /locus_tag="VF402_08890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7800451.1"
                     /translation="MPMRIVPVEPDHIRALSELAQVSFTETFGYLYPPEDLAHYLHKA
                     YAGPALAAEAADPLQFWRIAFDEDGRAAGYLQCGPVSLPHPDADPSREGELKRLYIHS
                     DFQGRGLGRGLLNLALAHLSERYGAAAQWIGVWSRNHKAQKLYASAGFEKVGDYLFAV
                     GDTRDAEFILRKAP"
     gene            complement(2125..>2338)
                     /locus_tag="VF402_08895"
     CDS             complement(2125..>2338)
                     /locus_tag="VF402_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7800452.1"
                     /translation="SGKPLAVIDVTDEQLAAGLKAAGLPEAIIPMLVSFDANTREGGF
                     DVLTGDVEALTGHKPRSLAAFFKAAL"
BASE COUNT          373 a          758 c          811 g          396 t
ORIGIN      
        1 ctgggcctga tctggttccc gaagaacctg ccgaacgtcc tgaacggcct gggcttccag
       61 ggcagcgtca ccacgctggc ctcgtcgcag aacgtgccgg acaccgacgc ggcgggcaat
      121 atcgtcgccc agctcaagac gccgttcttc ggcgtgtcgc gctattccta caacgcgacc
      181 ctggcttacg acaaaggccc gatcggcgcg cgtctgtcct atgtctggcg ctccggcttc
      241 ctggccagca acgaggccag cctgttcgcc aacccgctgg gcatctggcg tcatccggaa
      301 aagagcctgg acttccagtt gacctacaat atcaacgacc gcatgtcgtt cgatatcgac
      361 gccgtcaatc tcaccaacga gatgcagcag caatattacc actttggcga cgccggttcg
      421 cccgaactga cggatttcgg cacggtccag atcggacgct ccgtctccgt cggcttccgc
      481 tggaaaatgt aagcgtacgc gtttcgggac cgggcgtatc ctccggtccc gctcatcctc
      541 ttcgagcttt cccgacctgg tcaggcgttg cggtcctccc gcaacgcctg ctttttttgc
      601 gatttcgccg ccgcggatcg tgtatagacg ccttatgacc ctggagcagt tgcgcatctt
      661 cgtcgcggtg gccgagcgcg agcatctgac gcgcgcggcg gagagcctgt gcctgacgca
      721 atcggcggtc agcgccgccg tcgcgcaact ggaggcgcgc ttcggggtga agctgttcga
      781 ccgcatcggg cgcggcatcg cgctgaccga ggccgggcgt ttgttcctgc ccgaggcgcg
      841 ggccgtgctg gcgcgcgcgc atgaggccga ggcgctggtg cgcgacgtgg ccggggcgct
      901 gcgcgggagt ttgcgcatct gcgccagcca gacggtcggc aatttctggc tgccggcgcg
      961 gctggcgcgc tttcaggcgg cgtttccggg gctggtgctg catgtcgaac acctgaacac
     1021 cgagcgcgcg gcggcgaagg tgctggccca cgaggccgac atcgccgtgg tcgagggcgg
     1081 gatcggcgat ccgcgcctga gcgcgacggc gctcagcggc gaccggatgc tgctggtgct
     1141 gcccaacggc tgggccgcgc cgcgcgcgcc ggaggactgg gcggcgctga cgttcgtgac
     1201 gcgcgagccg gggtcgggca cgcgggcgct gctggccgat cacctgaacg ggatggggct
     1261 gcggctggac gcgcgcaaca tcgcgctgga gctgccgtcg aacgaggcgg tgcgcggcgc
     1321 cgtcgaggcg ggcgccgggg ccagcctgct gtcggaactg gtgacgcaaa gggcgctgcg
     1381 caccggcgac gtgcagggga tcgacatcgg cctgccgccg cgcccgtttt acgcgctgcg
     1441 gctgaaagaa cgcgccggca cgcgggcgga aagcgaattc atcagggcgc tttgcgcagg
     1501 atgaattccg cgtcgcgcgt gtcgccgacc gcgaacaggt aatctccgac cttctcgaaa
     1561 cccgccgagg cgtagagctt ttgcgccttg tggttacggc tccatacgcc gatccattgc
     1621 gccgccgcgc cgtagcgctc gctcaggtgc gcgagcgcga gattgagcag gccgcggccc
     1681 aagccgcgcc cttgaaaatc ggaatggatg tagagccgct tcaactcgcc ctcgcgcgaa
     1741 ggatcggcgt ccgggtgcgg caggctgacc gggccgcatt gcaggtaacc ggcggcgcgg
     1801 ccgtcctcat cgaacgcgat gcgccagaat tgcaagggat cggcggcctc ggcggccagc
     1861 gccggtccgg cgtaggcttt atgcaggtag tgcgcgaggt cttccggcgg atagagataa
     1921 ccgaaggtct cggtgaacga gacctgcgcc agttcggaca gggcccggat atgatcgggt
     1981 tcgacgggaa cgatgcgcat cggcatgggt tagcggatcg gtcaggcgcg ggcaagattg
     2041 gccacgaaag gacacgaaag gacacgaaat tcaggctaca gcccggaatt tcgtgccttt
     2101 tcgcgccttt cgtggccaaa atgcctataa cgccgccttg aagaacgccg ccagcgaacg
     2161 cggcttatgg ccggtcaggg cctccacatc gccggtcagc acgtcgaagc cgccttcgcg
     2221 ggtgttggcg tcgaaggaga ccagcatcgg gatgatcgct tcgggcagcc ccgccgcctt
     2281 cagaccggcg gccagttgtt cgtccgtcac gtcgatgacg gccagcggct tgcccgac
//
LOCUS       DASVLL010000537         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_411208,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000537 DASVLL010000000
VERSION     DASVLL010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411208"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..2413)
                     /locus_tag="VF402_08900"
     CDS             complement(11..2413)
                     /locus_tag="VF402_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7800453.1"
                     /translation="MPSHRRPTSPIPSLRRLGLICGVCALALTGPSHAQDQDDGGPNG
                     GPIPYNQLGTTPEPVAVHGPTPLSASDSALLAQAIAAVKSRDFAGADAMAANLSDPVA
                     RKIVTWTIINNSVDTGGIYAFARLDAARRDLWGWPRAANRQIAAEKMIATAGLTPQQV
                     ADWFNGAPPQSLPGATALINAYIALNRVPEAQALAKQWWRAQVFDAIGQANFYQAFGR
                     FLTPDDSVARIDCLILDTQNASSQTISAMIPYVDATTAAVANAAIAMRSGAASADGLY
                     QSALALDPHNKVLAYARARYLSSKGLEPLGFGLLADLPPASMSPGAATQLYRTRLVYF
                     RAALKAGNYPAAYAAMNDGGFDGGEPQAEADFFAGWIALQKLNRPDDAIAHFQGVAAA
                     GTSPITQGRADYWLGRAYEARNQPGDAQIAHDWYVKGSQYIYAFYGQMAAEKAGVTQI
                     SLGRDPVPSAADKARFEGREEVRAARMLGAIGETGLFRAIVLDLDTVLPNAEEEALLV
                     DLTATYDSQTIAMKVARMSLQRGFYLPERAFPIRDLPNVPAPEKAYVLAITRQESGFD
                     PSVRSPANARGMMQLIPSTARAVANRLGIGYSESQLYQPDYNMTLGAYHLGELVSRMG
                     GSYILASAGYNAGPSRMGQWVAQCGDPRGQGADALSFIECMPLTETRNYMMRVTENMR
                     VYRARLNGGTAPLTATADITQGAPAPFGAFDASDVGDGSLPDGPISYADYQKAQARDA
                     AAAAALAAAPKAEPKVEPKVEHESKRKIAEKKKKAERKKAESKKKTAHAKKSAERRKK
                     RR"
     gene            2590..>2848
                     /locus_tag="VF402_08905"
     CDS             2590..>2848
                     /locus_tag="VF402_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7800454.1"
                     /translation="MSDAIAAPLFRGVLTALVTPFRNGEVDYDAFRAQVERQIAAGVH
                     GLVPVGTTGETSTLSMEEHKAIVKLCVETAAGRVRVIAGAGS"
BASE COUNT          416 a          903 c         1042 g          487 t
ORIGIN      
        1 ccacatcgga tcagcggcgt ttcttgcgcc gctcggcgct ctttttggcg tgggcggttt
       61 tctttttgct ctcggccttt ttccgttcgg ccttcttttt cttctccgcg atcttccttt
      121 tcgactcatg ctcgacctta ggctcgacct taggctcggc cttgggcgcg gcggccagag
      181 cggcggccgc ggccgcgtcc ctggcctgcg ccttctgata gtcggcatag gagatcgggc
      241 cgtcgggcag cgagccgtcg ccgacgtccg aggcgtcgaa ggcgccgaac ggcgcgggcg
      301 cgccctgcgt gatgtccgcc gtcgcggtca gcggcgcggt tccgccgttc agccgggcgc
      361 gatagacgcg catgttctcg gtcacgcgca tcatgtagtt gcgcgtctcg gtcagcggca
      421 tgcactcgat gaaggacaag gcatccgcgc cttgcccgcg cggatcgccg cattgcgcga
      481 cccactggcc catgcgcgac ggcccggcgt tatagcccgc tgaggcgagg atgtaggagc
      541 cgcccatgcg gctgaccagc tcaccgaggt gataggcgcc cagggtcata ttgtagtcgg
      601 gctggtaaag ctggctttcc gaatagccga tgccgagccg gttggcgacg gcgcgggcgg
      661 tcgaggggat caactgcatc atgccgcgcg cgttggcggg cgagcgcacg gaagggtcga
      721 agcccgattc ctgacgcgtg atggccagca cataggcttt ttccggcgcc ggaacattgg
      781 gcaggtcgcg gatcggaaac gcgcgttcgg gcaggtagaa gccgcgttgc agcgacatgc
      841 gcgccacctt catggcgatg gtctgcgaat cgtaggtcgc ggtcaggtcg accagcagcg
      901 cctcctcctc ggcgttgggc agcacggtgt cgaggtcgag gacgatggcg cggaacaggc
      961 ccgtctcgcc gatggcgccc agcatgcgcg ccgcgcgcac ctcctcgcgc ccctcgaagc
     1021 gcgccttgtc ggcggcggag gggacgggat ccctgcccag cgaaatctgc gtcacgcccg
     1081 ccttttccgc cgccatctgg ccgtagaagg cgtagatgta ctgactgccc ttgacgtacc
     1141 agtcgtgggc gatttgggcg tcgccgggct ggttccgggc ctcataggcg cggcccagcc
     1201 agtagtcggc gcggccctgc gtgatcggcg acgtgcccgc cgccgccacg ccctgaaaat
     1261 gcgcaatcgc gtcatcgggg cggttcagct tttgcagcgc gatccagccc gcgaagaagt
     1321 cggcctcggc ctgcggttcg ccgccgtcga agccgccgtc gttcatggcg gcgtaggcgg
     1381 cggggtaatt gcccgccttg agcgcggcgc ggaaatagac gaggcgcgtg cggtaaagct
     1441 gcgtcgccgc gccgggcgac atcgaagcgg gcggcaggtc ggccagcagc ccgaagccga
     1501 gcggctccag ccccttggac gacaggtagc gcgcgcgggc gtaggccaga accttgttgt
     1561 gcggatcgag ggcgagggcg ctctgataca ggccgtcggc gctggcggcg ccggagcgca
     1621 tggcgatggc ggcgttggcg acggcggcgg tcgtggcgtc gacatagggg atcatcgcgc
     1681 tgatcgtctg cgacgaggcg ttctgcgtgt ccagaatcag gcaatcgatg cgcgccacgc
     1741 tgtcgtccgg ggtcaggaag cgcccgaacg cctgatagaa gttggcctgg ccgatggcgt
     1801 cgaacacctg cgcgcgccac cattgtttcg ccagcgcctg cgcctccggg acgcggttca
     1861 gcgcgatata ggcgttgatc agggcggtgg cgccgggcag gctttgcggc ggcgcgccgt
     1921 tgaaccagtc ggcgacctgc tgcggcgtca ggcccgccgt cgcgatcatc ttttcggcgg
     1981 cgatctggcg gttggcggcg cgcggccagc cccacaggtc gcggcgcgcg gcgtcgagcc
     2041 gggcgaaggc atagatgccg cccgtatcga cgctgttgtt gatgatggtc caggtgacga
     2101 tcttgcgcgc caccggatcg ctcagattgg ccgccatcgc gtcggcgccg gcgaaatcgc
     2161 gcgacttcac ggccgcaatc gcctgcgcca gcagggcgga atcgctggcc gacagcggcg
     2221 tcgggccgtg gacggcgacc ggttccggcg tcgtgccgag ctggttgtag gggatggggc
     2281 cgccattggg gccgccgtcg tcctggtcct gcgcgtgcga cgggccggtg agggccagcg
     2341 cgcatacgcc gcagatcagc cccagccggc ggagagacgg gatcgggctg gtcggacggc
     2401 gatgggacgg catggggatt ccttcggtga cgcgatgcgg gaactataga tgatagtttc
     2461 tttttagaaa gccattgcgg cgaaagcgca gccgccgtaa ggctaatccg cttatagata
     2521 gcgccggacg caggggccaa gacacaagtc gcggccgcct gatcacggtt tctggatcac
     2581 ggattgacga tgagtgacgc aattgccgcc cccctgttca ggggcgttct gacagccctg
     2641 gtgacgccct tccgcaatgg cgaggtcgat tacgacgcct tcagggcgca ggtcgagcgc
     2701 cagatcgcgg cgggcgtgca tgggctggtg ccggtcggca ccacgggcga gacctcgacc
     2761 ctgagcatgg aagagcacaa ggccatcgtg aagctgtgcg tcgaaaccgc cgccgggcgc
     2821 gtgcgggtga tcgcgggcgc gggctcga
//
LOCUS       DASVLL010000538         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_35440, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000538 DASVLL010000000
VERSION     DASVLL010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35440"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..>1605)
                     /locus_tag="VF402_08910"
     CDS             complement(119..>1605)
                     /locus_tag="VF402_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX7800455.1"
                     /translation="KTIDLQPDIFFLLFLPPLLFIEGWRIPKAGLLTDWRLILQMAIG
                     LVVFTVIGGGLIIHWLIPDMPLTVAFALAAVISPTDPVALSAIARGRAIPPRLQHILE
                     GESLLNDASGLVCLRVAVGVVVTGSFSLVHASGLFVWLAACGIACGVAMTLLVGFLKG
                     RLTRWLGEEPGTEILISLLLPMGAYLLAEALHGSGILAAVAAGIAMSYTEQLGRALPA
                     TRLQRGAVWDTVQFALNGVIFVLLGEQFPSIIARAERLVAVTGHRDPIWMLYYILAIT
                     VALAVLRFFWVWLSFTLTRFRRGSKPIPTNFRLIAVTSVAGVRGARTLAGVLTIPFAV
                     AGGGPFPVRNLVIFLAAGVIIASLLIATVLLPVLLKGLSLPQEDAASGRVAQARIAAA
                     RAAIKAIETLRGAGADHEAEAYAAMSERALQPYADRLKGMEDEAKTPEQRRALAQVER
                     DFRRAALRAERDAYYHMGRAGELPDDAVRKLVSEIDLLEERLNS"
BASE COUNT          273 a          542 c          579 g          211 t
ORIGIN      
        1 cggcggcggc ggtgaggaac ggtttgcgca tggaatctcc cccggagcgt tgcaggccgg
       61 actcaagagc ggtccggtgg ggagagccta gccccattct ttgcaactgg aaagtgaatt
      121 acgagttcag gcgctcctcc agcaggtcga tttcgctgac cagcttgcgc accgcgtcgt
      181 cgggcagctc gccggcgcgg cccatatggt aataggcgtc gcgttcggcg cgcagggcgg
      241 cgcggcggaa gtcgcgctcg acctgcgcca gcgcgcggcg ctgttcgggg gttttggcct
      301 cgtcctccat gcccttgagc cggtcggcat agggttgcag cgcgcgttcg ctcatggcgg
      361 cgtaggcttc ggcctcgtga tcggcgccgg cgccgcgcag ggtctcgatg gccttgatcg
      421 ccgcgcgcgc cgccgcgatg cgcgcctgcg cgacgcgccc ggacgccgcg tcctcctgcg
      481 gcaggctgag gcccttgagc aggacgggca gcagcacggt ggcgatcagc agcgaggcga
      541 tgatcacccc cgccgccagg aagatgacga ggttgcgcac cgggaacggc ccgccgcccg
      601 cgaccgcgaa cgggatggtc agcacgcccg ccagagtccg cgcgccgcgc actcccgcca
      661 ccgaggtgac cgcgatcagg cggaaattcg tcgggatcgg cttcgatccg cgccggaagc
      721 gcgtcagggt gaaggacagc cacacccaga agaagcgcag gaccgccagc gccacggtga
      781 tggccagaat atagtacagc atccagatcg ggtcgcggtg gccggtgacc gcgaccagac
      841 gctcggcgcg cgcgatgatc gacggaaact gctcgcccag cagcacgaag atgacgccgt
      901 tgagcgcgaa ctgcaccgtg tcccagaccg cgccgcgttg cagccgcgtg gcgggcaggg
      961 cgcggcccag ttgctcggtg tagctcatgg cgatcccggc ggcgacggcg gccaggatgc
     1021 cggagccgtg cagcgcctcg gccagcaggt aggcgcccat cggcagcagg aggctgatca
     1081 ggatttccgt gcccggctcc tcgcccagcc agcgcgtcag gcggcccttg aggaagccga
     1141 ccagcagggt catcgccacg ccgcaggcga tgccgcacgc cgccagccac acgaacaggc
     1201 cggaggcgtg gacgaggctg aacgacccgg tcaccaccac cccgaccgcc acgcgcaggc
     1261 agacgaggcc cgatgcgtcg ttgagcagcg attcgccttc gagaatgtgt tgcaggcgcg
     1321 gcggtatggc ccgcccgcgc gcgatcgccg acagcgcgac cggatcggtc ggcgagatca
     1381 ccgccgccag cgcgaacgcc acggtcagcg gcatgtcggg gatcagccag tggatgatga
     1441 ggccgccgcc gatcaccgtg aacacgacga ggccgatcgc catttgcagg atcagccgcc
     1501 agtcggtgag cagcccggcc ttggggatgc gccagccctc gatgaacaaa agcggcggca
     1561 ggaacagcag gaagaagatg tccggttgca ggtcgatggt cttgc
//
LOCUS       DASVLL010000539         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_90097, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000539 DASVLL010000000
VERSION     DASVLL010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90097"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VF402_08915"
     CDS             complement(<1..640)
                     /locus_tag="VF402_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7800456.1"
                     /translation="MPISDIQSLAGALRAEIGKAVIGQDDTLDLMLTALFSGGHILLE
                     GPPGTAKTLLARSFCRALSLRFGRIQFTPDMLPGDVLGTSLFNFQTSAFTLSKGPIFC
                     DLLLADEINRTPPKTQAALLEAMQERAVTLDGARHDLGARFTVIATQNPIEQQGTYPL
                     PEAQLDRFLFKHVLGYPSLEEERRIVEGAGNAQASDNALDALRPVLDGAALDA"
     gene            complement(627..2255)
                     /locus_tag="VF402_08920"
     CDS             complement(627..2255)
                     /locus_tag="VF402_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800457.1"
                     /translation="MARFSLPKSVNLKAADWLPGVALFLCLGLFAMAGVLLIGANADP
                     VTGQSAYVTGVSAQGYQGLKRLLAARGHAVAVNRDEDGPARARADLEIITLDGDNANL
                     VFGVDQDLMDGASSSASASASSASASASSAGPAAPDRDYYYPAPDPKRADHILYHPLG
                     KAVLVVAPKWSAARMEKHPLWDVDPTPIPAAIVRDNLEVLAPAEESVAPLMPGKPADL
                     PPPPPGRARLQSLRRLIVFDIPHYEIRRDARPRTAAEKAAAQKAAQALPKGFAPGVDA
                     ANTDVPVRRLWRASGSVSGGAYDLGAISGFQSITAPNLQPVLTTQDGRPVLSRLIVTG
                     GRRQPVVPVYVLSEPDLLNNTILADPQRAANALNLIEALSGRRASIVFNLTFAGMGFD
                     HDLLHTLARPPYVAAPLCLLLMGVTLMWAAFSRFGPPLPAPEAAPLGRGVRLLADNAA
                     RLFALTMKQPKLAGAYAQHVRDMVLRRRGSDGPAPGGEPPDELAERIGRAHGATDSYL
                     DLKARADKARTVHQLIDITRRLHAWKTEIDRAHI"
     gene            complement(2256..2981)
                     /locus_tag="VF402_08925"
     CDS             complement(2256..2981)
                     /locus_tag="VF402_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800458.1"
                     /translation="MTQGSHTADGAASLSGGPLVRGPVDAQSAARLIAQANARHDLQS
                     RFGASDAPDARTIAFWEHVRDAVQRFFDALGHLLKPIAPALPYILWGLLILVVALILS
                     PVVRMFVTSRFERFFARHGLRADAEWRPSQQAVAALLAEIDALAAKGDYDEAVHLLLM
                     RSVADINAFRPDLAPRHFSARDILRHPLLPEAARPAFADIVRWVERSFFAGIRVGEAG
                     FNACRAAYVRFAAAEGLAPRGGV"
BASE COUNT          560 a         1243 c         1241 g          526 t
ORIGIN      
        1 ccgcgtccag cgccgccccg tccagaaccg ggcgcagggc gtccagcgcg ttgtcgctgg
       61 cctgcgcgtt gcccgccccc tcgacgatgc ggcgctcctc ctccagcgac ggatagccca
      121 gcacgtgctt gaacaggaag cggtcgagct gcgcctccgg cagcggatag gtgccctgct
      181 gctcgatcgg gttctgcgtc gcgatgacgg tgaaacgcgc ccccaggtcg tggcgcgcgc
      241 cgtccagcgt caccgcccgc tcctgcatgg cctccagcag cgccgcctgc gtcttgggcg
      301 gcgtgcggtt gatttcgtcg gccagcagca ggtcgcagaa gatcggcccc ttgctgagcg
      361 tgaaggccga ggtctggaag ttgaacaggc tcgtgcccag cacgtcgccc ggcagcatgt
      421 ccggcgtgaa ctggatgcgc ccgaagcgca gggacaaggc gcggcagaag ctgcgcgcca
      481 gcagcgtctt ggccgtgccg ggcgggcctt ccagcaggat atgcccgccg gagaacaagg
      541 cggtcagcat caggtccagc gtgtcgtcct ggccgatcac cgccttgccg atctcggcgc
      601 gcaaggcgcc cgccagggat tgaatgtcag atatgggcac ggtctatctc cgtcttccag
      661 gcgtgcagcc tgcgggtgat gtcgatgagc tgatgcacgg tgcgcgcctt gtcggcgcgc
      721 gccttgaggt ccagatagct gtccgtcgcg ccgtgggcgc ggccgatgcg ctcggccagc
      781 tcgtccggcg gctcgccgcc cggcgccggt ccgtcggaac cgcgccgccg cagcaccatg
      841 tcgcgcacat gctgggcgta ggcgcccgcc agcttcggct gcttcatggt cagggcgaac
      901 aggcgcgcgg cgttgtcggc cagaagccgc acgccacgcc ccagcggcgc cgcctccggc
      961 gcgggcaagg gcgggccgaa gcgcgaaaac gccgcccaca tcagcgtcac gcccatgagc
     1021 agcaggcaca ggggcgcggc gacatagggc ggacgcgcca gggtgtgcag cagatcgtgg
     1081 tcgaagccca tgcccgcgaa ggtcaggttg aacacgatgc tggcccggcg gccggacagg
     1141 gcctcgatca ggttcagcgc attggccgcg cgttgcggat cggccaggat ggtgttgttc
     1201 aaaaggtccg gctcgctcag cacatagacc ggaacgaccg gctggcgccg cccgccggtg
     1261 acgatcagcc gcgacagcac cggccgcccg tcctgcgtcg tcagcaccgg ttgcagattg
     1321 ggcgcggtga tggattgaaa accgctgatc gcgcccagat cgtaggcgcc gccagaaacg
     1381 ctgccggacg cgcgccagag gcggcgcacg ggcacatccg tgttcgcggc gtcgacgccg
     1441 ggcgcgaacc ccttgggcag ggcctgagcc gccttttgcg ccgccgcctt ctccgccgcc
     1501 gtgcgcggcc gcgcgtcgcg ccggatctca tagtgcggaa tatcgaagac gatcagccgc
     1561 cgcaggctct gcaaccgcgc ccgccccggc ggcggcggcg gcaggtcggc gggcttgccc
     1621 ggcatgagcg gggcgacgct ttcctccgcc ggggccagca cttcgagatt gtccctgacg
     1681 atggcggcgg ggatcggcgt cggatcgacg tcccagagcg gatgcttctc catccgcgcc
     1741 gccgaccatt tcggcgccac caccagcacc gccttgccga gcggatgata gaggatgtgg
     1801 tcggcgcgtt tcggatcggg cgccggataa taatagtccc ggtccggcgc cgccgggccg
     1861 gcggatgaag ccgaagccga agccgaggac gcggacgcgg acgccgagga cgacgcgccg
     1921 tccatgaggt cctgatcgac gccgaacacc agattggcgt tgtcgccgtc cagcgtgatg
     1981 atctccagat cggcgcgggc gcgcgccggt ccgtcctcgt cgcggttgac ggcgaccgcg
     2041 tggccgcgcg ccgccagcag ccgtttcagc ccctgatagc cctgcgccga cacgccggtg
     2101 acataggccg actggcccgt gaccgggtcg gcgttggcgc cgatcagcag cacgcccgcc
     2161 atcgcgaaca ggcccaggca caggaacagg gccacgccgg gcagccagtc ggcggccttg
     2221 agattcaccg atttgggcag ggaaaagcgc gccatctaca cgcctccccg cggcgccaga
     2281 ccctcggcgg cggcgaagcg gacataggcg gcgcggcagg cgttaaagcc cgcctcgccc
     2341 acccggattc cggcgaaaaa ggaccgctcc acccagcgca cgatgtcggc gaaggccggg
     2401 cgcgccgcct ccggcagcag cggatggcgc agaatgtcgc gcgccgagaa atgcctgggc
     2461 gccaggtcgg gccggaaggc gttgatgtcg gccacgctgc gcatcagcag caggtggacc
     2521 gcctcgtcat agtcgccctt ggccgccagc gcgtcgatct cggccagcag cgccgccacc
     2581 gcctgttggc tgggccgcca ttcggcgtcg gcgcgcaggc cgtggcgcgc gaaaaagcgc
     2641 tcgaagcgcg aggtgacgaa catgcgcacg accggcgaca ggatcagcgc cacgacgagg
     2701 atcagcaggc cccacagaat atagggcaag gcgggcgcga tcggcttgag caggtggccc
     2761 agcgcgtcga aaaaccgctg cacggcgtcg cgcacatgct cccagaaggc gatggttctg
     2821 gcgtcgggcg cgtccgaggc gccgaagcgg ctttgcaggt cgtggcgggc attggcctgc
     2881 gcgatcagcc gcgcggcgga ttgcgcgtcc accggccccc tgacaagtgg ccccccggaa
     2941 aggctggctg cgccgtcggc cgtgtgcgaa ccctgcgtca cctgaaaccc gtctccccct
     3001 cgcgcgctcc gcccccaaag ccgcacaccg cgctttaccg gaccacgtct tccgaagcgc
     3061 gggccccgga tgcgcgccat atgtcagact aagcgggaaa gcgcacaccg gtcaagccgc
     3121 ctctgccttt gggggagcgc gggtctggcc tgtttgcggg ccagagaccc gcgcggcgcg
     3181 cgccttcgtc tccgttttca cccttagaga gtctgttaga agagtcttag ataagttaag
     3241 gcgcggcgaa agcttatatt tcaagccttt gccgcgcctt ccggtgtgtg atgccccgtg
     3301 cttcggtgtg tgatgccccg ccgccgaagt gggtcatacc ccgtcttaaa agtgggcgat
     3361 cccccgttgg ataagttgag tttttggcgt tttccacagg cttatcccca ggcggcggcg
     3421 gtgtgtaatc ccccgatgga cggtttgagg cgctttcaac gcccccgcgc gccgttttcg
     3481 ccgcccccga agcggtgggc gatcccccga agccccgcgc gactcggccc gttcgggcgc
     3541 aaaaccttaa gaaagtccta acgcatgact
//
LOCUS       DASVLL010000540         6257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_146597,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000540 DASVLL010000000
VERSION     DASVLL010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146597"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF402_08930"
     CDS             complement(<1..285)
                     /locus_tag="VF402_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX7800459.1"
                     /translation="MSADTLTLSRHDFTVGDKVVYPAHGVGSVAAVETQEVAGLSLEV
                     YVITFDHEKMTLRVPTKKAKTAGLRHLAAEAVMSQALTTLKGRARIKRTMW"
     gene            complement(721..1059)
                     /gene="fdxA"
                     /locus_tag="VF402_08935"
     CDS             complement(721..1059)
                     /gene="fdxA"
                     /locus_tag="VF402_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX7800460.1"
                     /translation="MTYIVTDPCVKCKFMDCVEVCPVDCFYEGENFLVINPDECIDCG
                     VCEPECPVDAIKPDTEDEGTKWLEINTKYAAVWPNISEKGTPPADREDYERETGKFEK
                     YFSEKPGDGK"
     gene            complement(1173..1466)
                     /locus_tag="VF402_08940"
     CDS             complement(1173..1466)
                     /locus_tag="VF402_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX7800461.1"
                     /translation="MSDPSPSVRLDVWLWRARFFKTRALAAKACEAGAIRLERFGRIG
                     RVEKAALNVRPGDRLIFALGARLIDVEIRAAGERRGPASEAQTLYVIRENPPA"
     gene            1607..4072
                     /locus_tag="VF402_08945"
     CDS             1607..4072
                     /locus_tag="VF402_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX7800462.1"
                     /translation="MRRTLLMAASLLVMAGAAQAEPQLKLNDKGYFETPGLNVTVFAD
                     IYPDGHQTGVTVIQHGTRVAANGDLRLEPSPGQWSPMPAGDGKPVVDAATRTVTQTLH
                     YPDESKNRKGFNPIDYPDLNFTYHVAVTPMDGDSFKITVDLDKPLPADWEGKVGFNFE
                     LFPTDLFGKSWLMDAQSGTFPRQADGPMRDDHDQLIAAPMAEGKTLVVAPESDLQRLK
                     IESQTGQLELLDGRGNMNNGWYIVREITRPGATKNAVSWIVTPNVDPNWRSAPVVQVS
                     QVGYRPGQAKTVVIEQDKRDDKADPLILYKLTGNGPLEVASFTPKPWGQFLRYNYLTA
                     DLSAVKDPGVYELGYRGKMTHVFRISDDVYDRGVWQPTLDYFLPVQMCHMLVKENYRT
                     WHGLDHQDDARMAQVGDHFDGYEQGPDTLTQYKPGDRVPGLTSGGWHDAGDYDLRVES
                     QMGTVWLLAKMIEEFGLNYDATTIDETKKEADIHRPDGVNDAIQQIEHGLLSVLGGYK
                     AMGRTYRGIIEPTLTQYTLLGDISTQTDGLDYDPSLKPGEKTATTSGTPDDRWVFTED
                     NPDRELGTAAGLATAARVLKTANPQMAKESLDAAVDLYSHAIDRAGDVGARVFALAEL
                     AKTTNDPAYVRRLIAMKPEILAHIDKTGAMLSEAMPLIHDPAFVADVGAAVKTYQEKL
                     SADATKTPYGVPYVPYIWGAGWDIQKFGVDQYFFRKAWPQYTPDALQMEALNFVLGVH
                     PGDNPESFASGVGAQSATVAYGANRADWSYIPGGVISGTALIRPDLPELKIWPYFWQQ
                     REYVLGGGATNYMFLVLAAAQKK"
     gene            complement(4074..4364)
                     /locus_tag="VF402_08950"
     CDS             complement(4074..4364)
                     /locus_tag="VF402_08950"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7800463.1"
                     /translation="MAYKLTEAAEEDVIHLLAEGGRQFGLRQAHAYYAELLECFVFLA
                     ENPRAARERTDLSPVVRIHPYGSHVIIYQITVDDDVLILAVRHARENWQDDL"
     gene            complement(4367..4612)
                     /locus_tag="VF402_08955"
     CDS             complement(4367..4612)
                     /locus_tag="VF402_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX7800464.1"
                     /translation="MATMNISLPDPMKAWVEAQAETGKYSNSSDYVRDLIRRDQARAE
                     KIAAMQAVVTRSLASGIGARSMDQIWEEAQARIKAAG"
     gene            complement(4674..>6257)
                     /locus_tag="VF402_08960"
     CDS             complement(4674..>6257)
                     /locus_tag="VF402_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate-binding protein"
                     /protein_id="HEX7800465.1"
                     /translation="ADLDRMEGDIDTLSSRLSGVRTLTYIAHRGQWLKQTEHWRAVTR
                     DLEDRLSDRLHEALMQRFIDQRTSALLKALDAEEAPRPEVTPDGQVIIEGHTVGELKG
                     LSFRPGAADSLIADKALRQAAFRAVRPVLIDRLRALGQAASKTFRVKGHTVLWNNEAV
                     AEIEPSDYFAPRVRLIDAPDHPGLGANATKRVADYLRQLAQHQFKGLWKAKQAAEAAD
                     TLPHVRAIAFRLYENGGVLHRDETLKLSPEDKAALKALGVAGHRYAWFLPDMLSPRLR
                     PLLQAFGKAEGQRQHSRKGQLLLDDYGAVAMKTLSQIDKIMNTARYQKGAVYAKASEF
                     EALGLGEKQRDKLMTALGLVKVEPITVMEEVAVAAAEPEAPAKKKRRRKGRTAPAGEA
                     PLVAVEAETVEAAPEIAPEIAPESAPDAVATETPAEPAPPEPPVVEMRETQLIGWRQK
                     NHEPREPRRRPQPEATGEKPARKGKPQGRPERGGKRFDAPRNDRPQRKEPAVNPFSPF
                     ADLRARLNSKVEAEKVGQD"
BASE COUNT         1071 a         2027 c         2050 g         1109 t
ORIGIN      
        1 ccacatggtg cgcttgatgc gcgcgcggcc cttgagcgtg gtcagggcct gcgacatgac
       61 ggcctcggcg gccagatggc gcagaccggc ggtcttggcc ttcttcgtcg gcacgcgcag
      121 ggtcattttt tcgtggtcga aggtgatgac atagacttcg aggctgaggc cggccacttc
      181 ctgcgtttcg accgccgcga cggagccgac gccgtgcgcc ggatagacga ccttgtcgcc
      241 tacggtgaag tcatggcggg aaagggtgag ggtgtctgcg gacatggcga acctttcgcg
      301 ggtttgaggc gggtcggcgg aaggtccggt ctttcaggcg tcagaaaagc gttcccggct
      361 ccggggaggg ctccggcgat acgcaggacg cgcaaagtcc cgaacccgga gagtttttct
      421 caaggaccga gatggcgcgc gccaattcct gactaagaca aggttatcat ccgacctgcg
      481 tcgatggcgc gtccgaaacc ttcctgccgg tcaggcccga acgcttaaaa aaacaaacga
      541 agatttgcgc ggatcgtctg gtctctgcct tgcggggaag gcgcacgagg ccgctgcgac
      601 tcttgcaatg agcctgtgta tatcacaaat ttgcgacaaa ataaagcggc catccggaaa
      661 cgaccgcgaa aatgttaaga cattacagcg ggctttttgc ttttatgccg caatttcgcc
      721 ctatttaccg tcgccgggct tttcggagaa gtatttttcg aacttgccgg tttcgcgctc
      781 atagtcctcg cggtcggcgg gcggcgtgcc tttttcggag atgttcggcc agacggcggc
      841 gtatttggtg ttgatctcca gccacttcgt gccttcgtcc tcggtgtccg gcttgatcgc
      901 gtcgaccggg cattccggct cgcacacgcc gcagtcgatg cattcgtccg ggttgatgac
      961 caggaagttc tcgccctcat agaaacagtc gaccggacag acttccacgc agtccatgaa
     1021 cttgcatttc acgcaggggt cggtgacgat ataggtcatt ggaacgaact cttgaggcgg
     1081 atgcggactt ttcgcattgc gtcatgcgca agtcggaccg ctctgtcaag acgcctgagc
     1141 cccatttgtt ctgatcgcag gctttagggc ggctaagcgg gcggattttc ccttatgaca
     1201 taaagcgttt gcgcttcgga agccgggccg cgacgttcgc ccgccgcgcg gatttcgacg
     1261 tcgatcagcc gcgcgcccag cgcgaagatc agccggtctc cgggccgcac gttcagggcc
     1321 gccttttcga cgcgcccgat ccggccgaag cgctccagcc ggatcgcccc ggcctcgcac
     1381 gccttcgcgg ccagcgcccg cgtcttgaag aaacgcgcgc gccacagcca gacgtcgagg
     1441 cggacggaag gagagggatc gctcatggac gagatgctca tggcgcgata ttggaagccg
     1501 cgccgcaaaa gtcaatttgc ggcttgcggc ccgcgctggg cgcggctagt ttttgtaatg
     1561 gattacaaca aggccgcgca aacgggccgt ttgggaggat acaaggatga gacgcacgct
     1621 tctgatggcg gcaagcctgc tggtcatggc gggcgcggcg caggccgagc cgcagctcaa
     1681 gctcaacgac aagggctatt tcgagacgcc gggcctgaac gtcaccgtct tcgccgatat
     1741 ctatcccgac ggccaccaga ccggcgtgac cgtgatccag cacggcacgc gcgtggcggc
     1801 gaacggcgat ctgcggcttg agccgtcgcc gggccaatgg tcgcccatgc cggcgggcga
     1861 cggcaagccc gtggtggacg ccgccacccg gaccgtgacc cagaccctgc attacccgga
     1921 cgagtcgaag aaccgtaagg gcttcaatcc gatcgactac ccggacctca acttcaccta
     1981 ccatgtcgcg gtgacgccga tggacggcga ttcgttcaag atcacggtcg atctcgacaa
     2041 gccgctgccc gccgactggg aaggcaaggt cggcttcaat ttcgagctgt tcccgaccga
     2101 tctgttcggc aagtcgtggc tgatggatgc gcagagcgga acctttccgc gtcaggcgga
     2161 cggcccgatg cgcgacgacc acgatcagtt gatcgccgcg ccgatggccg agggcaagac
     2221 cctggtggtc gcgccggaaa gcgacctgca acggctgaag atcgaaagcc aaaccggcca
     2281 gctggagctg ctggacgggc gcggcaacat gaacaacggc tggtacatcg tgcgcgagat
     2341 cacccggccc ggcgcgacga agaacgccgt gtcgtggatc gtcacgccga acgtcgatcc
     2401 gaactggcgg tccgcgccgg tggtgcaggt gtcgcaggtc ggctatcgcc ccggtcaggc
     2461 caagaccgtg gtgatcgagc aggacaagcg cgacgacaag gccgatccgc tgatcctcta
     2521 caagctgacc ggcaatggcc cgcttgaggt ggccagcttc acgccgaagc cgtgggggca
     2581 attcctgcgc tacaactatc tgaccgcgga cctgagcgcg gtgaaagacc ccggcgtcta
     2641 tgaactcggc tatcgcggga agatgacgca tgtgttccgc atcagcgacg acgtgtacga
     2701 tcgcggcgtc tggcagccga ctctggacta tttcctgccg gtgcagatgt gtcacatgct
     2761 ggtcaaggag aattaccgca cctggcacgg cctcgaccac caggacgacg cgcgcatggc
     2821 gcaggtcggc gaccatttcg acggctatga acagggaccg gacacgctga cccaatacaa
     2881 gccgggcgac cgcgtgccgg ggctgacctc cggcggctgg cacgacgcgg gcgactacga
     2941 tctgcgcgtc gagagccaga tgggcacggt gtggctgctg gccaagatga tcgaggagtt
     3001 cgggctgaac tacgacgcca ccaccatcga cgagacgaag aaggaggccg acatccaccg
     3061 gcccgacggc gtcaacgacg cgatccagca gatcgagcac ggcctgctca gcgtgctggg
     3121 cgggtacaag gcgatgggcc ggacctatcg cggcattatc gagccgacct tgacgcaata
     3181 tacgctgctg ggcgacatct cgacccagac ggacgggctg gactacgacc cgtcgctgaa
     3241 gcccggcgaa aagaccgcca ccacgtcggg aacgcccgac gaccgctggg tgttcaccga
     3301 ggacaatccc gaccgcgaac tgggcacggc ggcggggctg gccaccgccg cgcgggttct
     3361 gaagaccgcc aatccacaga tggcgaagga gtcgctggac gccgccgtcg atctctacag
     3421 ccacgccatc gaccgggcgg gcgacgtggg cgccagggtg ttcgcgctgg cggagctggc
     3481 gaagacgacg aacgacccgg cctatgtccg gcgcctgatc gcgatgaagc cggagatcct
     3541 cgcccatatc gacaagaccg gcgccatgct gtccgaggcc atgccgctga tccacgatcc
     3601 ggccttcgtc gccgacgtcg gcgcggcggt gaaaacctat caggagaagc tgagcgcgga
     3661 cgcgaccaag accccctacg gcgtgcccta cgtgccctat atctggggcg cgggctggga
     3721 cattcagaag ttcggcgtcg atcagtattt cttccgcaag gcatggccgc aatatacgcc
     3781 ggacgccttg cagatggagg cgctcaactt cgtgctgggc gtgcatccgg gcgacaaccc
     3841 cgaatcgttc gcctccggcg tcggcgcgca atcggcgacc gtcgcctatg gcgccaaccg
     3901 cgccgactgg agctatatac ccggcggcgt gatctccggc acggccctga tccggcccga
     3961 cctgccggaa ctgaagatct ggccctattt ctggcagcag cgcgaatatg tgctgggcgg
     4021 cggcgcgacg aactacatgt tcctcgtgct ggcggcggcg cagaagaaat agcctacagg
     4081 tcgtcctgcc agttttcacg cgcatggcgc acggccagga tcagcacgtc atcgtcaact
     4141 gtgatctgat agatgataac gtgcgaacca tagggatgga tgcggacgac gggcgacaga
     4201 tcggtacgct ctcttgcggc gcgcggattt tcggccagga agacgaaaca ttccagcagt
     4261 tcggcataat aggcgtgcgc ctgccgcaga ccgaattgcc gtccgccctc ggcgagaaga
     4321 tggatgacgt cttcttccgc cgcctccgtc aatttatagg ccatatttaa cctgcggcct
     4381 taatgcgagc ctgagcctct tcccagatct gatccatgct gcgcgcgccg atcccgctgg
     4441 ccaggctgcg ggtgacgacg gcctgcatgg cggcgatctt ctcggcccgc gcctgatcgc
     4501 ggcggatcag gtcgcggaca tagtcgctgc tgttgctgta tttgccggtt tccgcctgcg
     4561 cctcgaccca ggccttcatc gggtcgggca gggagatgtt catggtcgcc atactgcgct
     4621 ccttccgccg acagactagg ccctttggca aagtttgtca aaatggcgcg aagtcagtcc
     4681 tgcccgacct tctcggcttc gaccttgctg ttcagccgcg cgcgcagatc ggcgaagggc
     4741 gagaaggggt tgacggccgg ctccttgcgc tggggccggt cgttgcgcgg cgcgtcgaag
     4801 cgcttgccgc cccgctcggg cctgccctgc ggcttgccct tgcgcgccgg tttttcgccc
     4861 gtcgcttcgg gctgcggacg gcggcgcggt tcgcgcggct cgtggttctt ctgccgccag
     4921 ccgatcagtt gcgtttcgcg catttcgacg acaggcggct cgggcggcgc cggttcggcg
     4981 ggcgtttccg ttgcaacggc gtcaggggcg ctctccgggg cgatctccgg ggcgatctcc
     5041 ggcgcggcct cgaccgtttc cgcctccacc gccaccagag gcgcttctcc ggcgggggcc
     5101 gtccggccct tgcggcggcg cttcttcttc gcgggcgctt ccggctcggc ggcggcgacc
     5161 gcgacctctt ccatcaccgt gatcggctcg accttgacca gccccagcgc ggtcatcagc
     5221 ttgtcgcgct gcttttcgcc gaggcccagc gcctcgaact cggacgcttt cgcatagacc
     5281 gcgcccttct gatagcgcgc cgtgttcatg atcttgtcga tctgcgacag ggtcttcatc
     5341 gcgaccgcgc cgtagtcgtc gagcagcaac tggcccttgc ggctgtgctg gcgctggcct
     5401 tccgccttgc cgaacgcctg caacagcggc cgcaggcgcg gcgacagcat gtcgggcagg
     5461 aaccaggcgt agcgatgccc ggccacgccc agcgccttga gcgccgcctt gtcctccggc
     5521 gacagtttca gcgtctcgtc gcgatgcagc acgccgccgt tttcatagag gcggaacgcg
     5581 atggcgcgca catggggcag ggtgtcggcg gcctcggcgg cctgcttcgc cttccacagg
     5641 cccttgaact gatgctgcgc cagttgccgc agatagtccg cgacgcgctt cgtcgcattg
     5701 gcgccgaggc cgggatggtc cggcgcgtcg atcaggcgca cgcgcggcgc gaaatagtcc
     5761 gacggctcga tctcggccac cgcctcgttg ttccacagca cggtgtggcc cttgacgcgg
     5821 aaggttttgg acgccgcctg ccccagcgcg cgcaggcggt cgatcagcac gggccgcacg
     5881 gcgcggaacg ccgcctgccg cagcgccttg tcggcgatca ggctgtccgc cgcgccgggc
     5941 cggaagctca ggcccttcaa ctccccgacc gtgtggccct cgatgatcac ctgaccgtcc
     6001 ggcgtgacct ccgggcgcgg cgcctcctcg gcgtcgagcg ccttgagcag cgccgaggtg
     6061 cgctggtcga tgaagcgctg catcagcgcc tcgtgcaggc ggtccgacag gcggtcctca
     6121 aggtcgcgcg tcacggcgcg ccaatgctcg gtctgtttca gccactggcc gcgatgggcg
     6181 atataggtca gggtgcgcac gcccgacagc cgcgacgaca gggtgtcgat atcgccctcc
     6241 atccggtcga gatcggc
//
LOCUS       DASVLL010000541         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_197751,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000541 DASVLL010000000
VERSION     DASVLL010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197751"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..>1522
                     /locus_tag="VF402_08965"
     CDS             333..>1522
                     /locus_tag="VF402_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7800466.1"
                     /translation="MQQRPRRPRDRAPLFQIGAILFQLEASLSLLADKRSAASKSRAR
                     TSTRGGRAQQPPRRNAVLARAVLACAAAGVEFVVVCCVALAIGMLYHAFAYDGSGLTA
                     PNLELWLLVAMLFVLSNAMRGDYRIAEYLSLRQYGWRLLSVWNASFLTALAFGFLAKS
                     TAESSRGVIVLFYVGGYFAVYATRLFLLRVARGATTPGGLAARRVFLVGLEANIENFT
                     HRYAPAACGMEIVAAVCLRDDEETIDEDLELAAAAARMLRPDDVFILTPWSRTQAIDR
                     CVDAFLRVPAQIHLGPESVLDRFVDARIDRIGAVSSLRLGGASLNPLEIAAKRCFDIV
                     VSSLGIALLSPLLLLVAALIKLDSPGPALFLQRRYGFNRESFRIFKFRSMTTMEDDRN
                     VAQAT"
BASE COUNT          226 a          531 c          457 g          308 t
ORIGIN      
        1 gccgaatgtc tccaccaccg tggcgatggc cgcgtccaaa cccgcgcggc gccaatgttc
       61 agcctgcagt aggatctcga attcgttcca cgccatgccg tgttccctgc cgagccttgc
      121 gccttggcgc ggcgcacgcc gcgctccaag aaaggaacgc tgacggcgcc acgccggtcc
      181 ctcgcgccct cacgaccatc gatcatggtc ggcggatgtt actttggtcg ccagggaaat
      241 aaaatcgatc gattgaggcg gcgcttgcgt ctttttgcgc gcctttggac ggcccgatcc
      301 ccctctcatg tgatcgtctg caggctcaac ccttgcagca gcggccgaga aggccgcgcg
      361 atcgcgcgcc cttgttccaa atcggcgcca tactgtttca attggaggct tccctgtcgc
      421 tgctcgccga caaacgttca gccgcgtcca aaagcagggc aaggacgtcc actcgcggcg
      481 gcagggccca gcagccgccg cggcgcaatg ccgtgctggc gcgcgcggtt ctcgcctgtg
      541 cggcggcggg agtggaattc gtcgtcgtct gctgcgtcgc cctcgccatc gggatgctct
      601 accacgcctt cgcctatgac ggcagcggcc tcacggcgcc caacctggaa ttgtggctgc
      661 tcgtcgcaat gctgttcgtc ctctccaacg cgatgcgcgg ggactaccgc atcgccgaat
      721 atctttcctt gcgccaatat ggctggcgcc tgctcagcgt ctggaacgcc agtttcctga
      781 ccgccctcgc cttcggcttt ctggcgaagt caacggccga atcttcgcgc ggcgtcatcg
      841 tgctgttcta cgtcggcggc tatttcgccg tttacgcgac acggctgttc ctgctgcggg
      901 ttgcgcgggg cgcgacgacc cccggcggcc tcgccgcgcg gcgcgtgttc ctcgtggggc
      961 ttgaagcgaa tatcgagaac tttacgcacc gttacgcgcc agccgcttgc ggcatggaga
     1021 tcgtcgccgc cgtgtgcctg cgcgacgacg aggagacaat agacgaagat ctggagctcg
     1081 ccgccgccgc cgcgcgcatg ctgcgccccg acgacgtgtt catcctgacg ccgtggtcgc
     1141 gcacgcaggc catcgaccgc tgcgtcgacg cctttttacg cgtgccggcg caaattcacc
     1201 tggggcccga aagcgtgctc gatcgcttcg tcgacgcgcg catcgacagg atcggcgccg
     1261 tatcgagcct ccgcctcggc ggcgcttcgc tcaatccctt ggagatcgcg gccaagcgct
     1321 gtttcgatat tgtcgtgtct tccctcggaa tcgccctcct ctcgccgctg ctcctcctcg
     1381 tcgcggcgct gataaagctc gatagtcccg ggcccgcgct gtttctgcag cgccgctacg
     1441 gcttcaatcg ggaatccttt cgcattttca aattccgctc tatgacgacg atggaggatg
     1501 atcgcaacgt cgcccaggcg ac
//
LOCUS       DASVLL010000542         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_146702,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000542 DASVLL010000000
VERSION     DASVLL010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146702"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VF402_08970"
     CDS             <1..611
                     /locus_tag="VF402_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX7800467.1"
                     /translation="MTSRQRANGKMKMHKTGGHMAGVVTALALAAAAAQPAQAQVSPQ
                     GGPIMVGSDTASMNQADHTQVLDGRVEIVQNDARLRADHVVMTYLPGQTDNSFGPIDT
                     IVATGNIYYVRPDSTVKGDKAVYTKSDDTMVVTGDVVLTQGQNVMTGNKLVSQVGKHI
                     TTLDANPVNGNPGRVHAVFYPDDKTKPGAKPAAAASSGASGH"
     gene            643..1407
                     /gene="lptB"
                     /locus_tag="VF402_08975"
     CDS             643..1407
                     /gene="lptB"
                     /locus_tag="VF402_08975"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX7800468.1"
                     /translation="MAPSAGAASSVSAEDDGIRVEGVGKTYKERAVVKSVSLSLRRGE
                     VVGLLGPNGAGKTTCFYMITGLIEADYGAIRLDGQDITQQPMYQRARMGVGYLPQEAS
                     IFRGMSVEENVMAVLELHDNNPQNVAEETTRLLEELHITHIRRSPATALSGGERRRVE
                     IARALAGKPSFILLDEPFAGIDPLAISDIRDVITYLKGRGIGILITDHNVRETLEIVD
                     RASIIHSGEVLFEGTAQEITHDPEVRRVYLGDSFTS"
     gene            1515..>2896
                     /gene="rpoN"
                     /locus_tag="VF402_08980"
     CDS             1515..>2896
                     /gene="rpoN"
                     /locus_tag="VF402_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX7800469.1"
                     /translation="MALGQRLELRQGQGLVITPQLQQAIKLLQLSNLELEAVIEAELE
                     RNPLLQRDDAESGPESGADPAAEADSAPERTDDARELTLSDRDAGAANNELDASDGDV
                     YGEDAPSVREREQTADAGEGPMVDWSKAGKGGGGFDGDDEGLERSLTRDKSLREHLTD
                     QALIAHFSTADMAVAQILIDGVDEAGYLRVSLDEIAERLGCGLDLVEDVLRRCQGFEP
                     TGVMARSVPECLKLQLIERNRFDPAMAALVEHLDLLARRDLSALRKVCGVDDEDLADM
                     IAELKSLNPRPGAAFDAEPSPAVTPDVFVRADVSGGWRIDLNSDTLPRVLVDRSYHAR
                     VAHSARSDEEKSYLHECLNQANWLIRSLDQRARTILKVSSEIVRQQDAFFVYGVEYLR
                     PLNLKTVAEAVGMHESTVSRVTTNKYIATPRGVFELKFFFTAAIASSDGTAAHSAEAV
                     RHKIKSLIDG"
BASE COUNT          546 a          997 c          916 g          437 t
ORIGIN      
        1 agatgacgtc acggcaaaga gcgaacggca agatgaagat gcacaaaact ggcggccata
       61 tggcgggagt tgtaacggcg ctggcgctgg ccgccgccgc ggcgcagccc gctcaggcgc
      121 aggtgtcgcc gcagggcggc ccgatcatgg tcggctccga caccgcctcg atgaatcagg
      181 ccgaccacac gcaggttctg gacgggcgcg tcgagatcgt tcagaacgac gcccgcctgc
      241 gcgccgatca cgtcgtcatg acctacctgc ccggccagac cgacaacagc ttcggtccga
      301 tcgacaccat cgtcgccacc ggcaacatct attatgtccg ccccgattcg accgtgaagg
      361 gcgacaaggc ggtgtacacc aagtcggacg acacgatggt ggtgaccggc gacgtggtgc
      421 tgacgcaggg ccagaacgtc atgaccggca acaagctggt gtcgcaggtc ggcaagcata
      481 tcaccacgct ggacgccaat ccggtgaacg gcaatccggg ccgcgtccac gccgtcttct
      541 atcccgacga caaaaccaag ccgggcgcca aaccggccgc cgccgcgtct tccggcgcat
      601 ccggccactg acagggaccg cttcgtgatc tccgactcgt tcatcgcgcc gtcagccggc
      661 gccgcttcgt ccgtttccgc cgaggacgac ggcatccgcg tcgaaggcgt cggcaagacc
      721 tataaggaac gcgccgtcgt caagagcgtg tcgctgtcgc tgcgccgcgg cgaggtggtc
      781 gggcttctgg ggccgaacgg cgcgggcaag acgacctgct tctacatgat caccggcctg
      841 atcgaggccg attacggcgc gatccgcctc gacggtcagg acatcaccca gcagccgatg
      901 tatcagcgcg cgcgcatggg cgtcggctac ctgccgcagg aagcgtcgat cttccgcggc
      961 atgtcggtcg aggaaaacgt catggcggtg ctggagctgc acgacaacaa tccgcagaac
     1021 gtggccgagg aaacgacgcg cctgctggag gaattgcaca tcacccacat ccgccgctcg
     1081 cccgccaccg ccctgtcggg cggcgagcgc cgccgcgtcg agatcgcgcg cgccctggcc
     1141 ggaaagccgt ccttcatcct gctggatgaa cccttcgcgg gcatcgatcc gctggccatc
     1201 tccgacatcc gcgacgtgat cacctatctg aaagggcgcg gcatcggcat cctgatcacc
     1261 gatcacaatg tccgcgaaac gctggaaatc gtcgaccgcg cctcgatcat ccactccggc
     1321 gaggtcttgt tcgagggcac ggcgcaggaa atcacccacg acccggaagt gcgccgcgtc
     1381 tatctcggcg actccttcac ctcctgacgc gcaaaaatcg tgccagttcg cggcggcggc
     1441 ggcgctttcc aagcaaatat taacgaacgc atgccacgct ggctccgaac cacaggtgac
     1501 ggacttcgcg acgcatggcg cttggacaac gtttagaact caggcaggga caggggctgg
     1561 tcatcacgcc ccagttgcag caggcgatca agctgctgca actgtccaat cttgagcttg
     1621 aggcggtgat cgaggccgaa ctggagcgca atccgctctt gcagcgcgac gacgccgaat
     1681 caggccccga atcgggagcc gacccggcgg cggaggccga ctccgccccc gaacgcacgg
     1741 acgacgcgcg cgaactgacc ctgtccgacc gcgacgcggg cgccgccaac aacgaactcg
     1801 acgccagcga cggcgacgtt tacggcgagg acgctccctc ggtgcgcgag cgcgaacaga
     1861 ccgccgacgc cggcgaaggc ccgatggtgg actggtccaa ggcgggcaag ggcggcggcg
     1921 gcttcgacgg cgacgacgag gggctggagc gcagcctgac gcgcgacaaa tccttgcgcg
     1981 aacacctgac cgatcaggcg ctgatcgcgc atttctccac cgccgacatg gccgtcgccc
     2041 agatcctgat cgacggcgtg gacgaggcgg gctatttgcg cgtctcgctg gacgaaatcg
     2101 ccgaacggct cggctgcggc ctcgatctgg tcgaggacgt gctgcggcgc tgtcagggct
     2161 tcgagccgac cggcgtgatg gcgcgcagcg tgcccgaatg tctgaaactg caactgatcg
     2221 agcgcaaccg cttcgatccg gcgatggcgg cgctggtcga gcatctcgac cttctggcgc
     2281 ggcgcgacct cagcgcgttg cgcaaggttt gcggcgtcga cgacgaagac ctcgccgaca
     2341 tgatcgccga gctgaaatcg ctcaatccgc gccccggcgc cgccttcgac gccgaaccgt
     2401 cgcccgccgt cacgcccgac gtcttcgtgc gcgccgacgt cagcggcggc tggcgcatcg
     2461 acctcaattc cgacaccctg ccgcgcgtgc tggtcgaccg cagctatcat gcgcgcgtcg
     2521 cccattcggc gcgctcggac gaggaaaaga gctatctgca cgagtgcctg aatcaggcca
     2581 actggctgat ccgcagcctc gaccagcgcg cgcgcaccat cctgaaagtg tcgtcggaaa
     2641 tcgtgcgcca gcaggacgcc ttcttcgtct atggcgtcga atatttgcgt ccgctgaacc
     2701 tcaagacggt ggccgaggcg gtcgggatgc acgaatcgac cgtgtcgcgc gtcaccacca
     2761 acaaatatat cgccaccccg cgcggcgtgt tcgagctgaa attctttttc accgccgcca
     2821 tcgcctcgtc cgacggcacg gcggcccatt ccgccgaggc cgtgcgccac aagatcaaga
     2881 gcctgatcga cggcga
//
LOCUS       DASVLL010000543         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_411546,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000543 DASVLL010000000
VERSION     DASVLL010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411546"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VF402_08985"
     CDS             <1..383
                     /locus_tag="VF402_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800470.1"
                     /translation="DAAVRAQADAARARAEARKHEDEIGRAKAVARKARAYAQALPPL
                     PAPPEAPAASRVVIRFGSGQGAVITVPAAPIAPTAPKIIIIHPPVAPPAPPVARLDAL
                     PAAAAKALPAPDAAADKVVVLAVR"
     gene            493..>1647
                     /locus_tag="VF402_08990"
     CDS             493..>1647
                     /locus_tag="VF402_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7800471.1"
                     /translation="MKRELRAADYETSLFQALAEAQKRFGARKIIIEDQDRKPLSYGL
                     FLTGAFALSRLLRRYLKDEPRVGLMLPTSVGGAVTFYALHALGKAPVMINFTSGQANI
                     RAAAQTAQIKTVITARRFVEQAKLEDLVAAMRAYVRVIYLDDIRKEVGLADKLYGLAA
                     SKAPALFAKKARPDDIGVILFTSGSFGSPRGVVLTQANLVANTRQIEAHIALKPEWTI
                     FNPLPIFHSLGLTGGIVLPLLAGLRSFQYPSPLHVKQIPDLLRETQSSLLFSTDTFLN
                     QYARAGTPEDFKSLEFIVWGAEKVRDETHNLFATQFGGIPILEGYGVTECSPVIAVNY
                     PDDNHFGTVGALLPGIEARLEPVEGIPVGGRLWVRGPNVMAGYINIDSPYV"
BASE COUNT          261 a          577 c          545 g          264 t
ORIGIN      
        1 gcgacgcggc ggtccgggcg caggccgacg ccgccagggc ccgcgccgaa gcacggaagc
       61 atgaggatga aatcgggcgc gcaaaggccg tggcgcgaaa ggcgcgggct tacgcccagg
      121 cgcttccgcc tctgcccgcg ccgccggaag cgcccgccgc gtcgcgggtc gtgatccgct
      181 tcggttcggg tcagggcgcg gtgatcacgg tccccgccgc gccgatcgcg ccgacggcgc
      241 cgaagatcat catcatccat ccgccggtcg cgccgcccgc tccgccggtc gcgcgcctcg
      301 acgccctgcc cgccgccgcc gcgaaagccc tgcccgcgcc ggacgcggct gcggacaagg
      361 tcgtggttct cgccgtgcgc tgagcgcgcg ccgatctttc ctttgacgct aaggtcaata
      421 cgtctatgat cgccggttca tgaccgccgg tcaaagcgac ggcggcgcgc ggggagttga
      481 caccggatgc gcatgaagcg cgaactgcgg gcggcggatt acgaaacgtc gctgtttcag
      541 gccctggccg aggcgcaaaa gcgtttcggc gccaggaaga tcatcatcga ggatcaggac
      601 cgcaagccgc tgtcctacgg gctgttcctg accggcgcct tcgccctgtc gcgcctgttg
      661 cggcgctatc tgaaggacga gccgcgcgtc ggcctgatgc tgccgacctc ggtgggcggc
      721 gcggtgacct tctacgccct gcacgcgctg ggcaaggcgc cggtgatgat caacttcacc
      781 tcggggcagg ccaatatccg cgccgccgcc cagaccgcgc agatcaagac cgtgatcacc
      841 gcccgccgct tcgtcgagca ggccaagctg gaagacctgg tcgcggcgat gcgggcctat
      901 gtgcgcgtca tctatctcga cgacatccgc aaggaggtcg ggctggcgga caagctgtac
      961 ggcctcgccg cgtccaaggc gcccgccctg ttcgcgaaaa aggcgcgccc ggacgatatc
     1021 ggcgtgatcc tgttcacctc gggcagtttc ggcagcccgc gcggcgtggt gctgacgcag
     1081 gcgaacctcg tcgccaatac gcgccagatc gaggcccata tcgcgctcaa gccggaatgg
     1141 accatcttca atccgctgcc catcttccat tcgctgggcc tgaccggcgg catcgtgctg
     1201 ccgctgctgg cgggcttgcg cagcttccag tatccgtcgc ccctgcacgt caagcagatc
     1261 cccgacctgc tgcgcgagac gcaatcctcg ctgctgttct cgaccgacac cttcctgaac
     1321 cagtacgcgc gcgccggaac gccggaggat ttcaaatcgc tggagttcat cgtctggggc
     1381 gccgagaagg tgcgcgacga gacgcacaac ctgttcgcga cgcagttcgg cgggattccg
     1441 atcctcgaag gctatggcgt caccgaatgt tcgcccgtca tcgcggtcaa ctatcccgac
     1501 gacaaccact tcggcacggt cggcgcgctt ttgcccggca tcgaggcgcg gctggagccg
     1561 gtcgagggca taccggtcgg cggccgcctc tgggtcaggg ggccgaacgt catggcgggc
     1621 tatatcaata tcgacagccc ctatgtg
//
LOCUS       DASVLL010000544         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_306302,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000544 DASVLL010000000
VERSION     DASVLL010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306302"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..452)
                     /locus_tag="VF402_08995"
     CDS             complement(21..452)
                     /locus_tag="VF402_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7800472.1"
                     /translation="MTDSLDIANLAADLRALVGRLKRRLREQGSVGDLTPSQIAVVRR
                     LDADGPATISALARAEGVRPQSMGATVAALEAAGLTQGRPDPDDGRQTLISLTPACRE
                     RLTQGRAARQDWLTRQIERLTPEERARLAAAADILRRLSES"
     gene            complement(648..1220)
                     /locus_tag="VF402_09000"
     CDS             complement(648..1220)
                     /locus_tag="VF402_09000"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEX7800473.1"
                     /translation="MRKPFLTAAAAALCLMAAPHMAAAQSATPAATPAAADPAPEAAT
                     PEKLALVHRMFGAMHIDAMMQGVMQQMTPALIEGLKKAHPELTGADSAKMQKAMTIVI
                     QVDQDYMPRILDATAQAYAEVFTTEELTAMDNFYESPVGQSMLAKTPQVMARVMPKVA
                     GFMPDMMAETQKRVCAEIDCSAKKDAAKTS"
     gene            complement(1308..>1510)
                     /locus_tag="VF402_09005"
     CDS             complement(1308..>1510)
                     /locus_tag="VF402_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800474.1"
                     /translation="LKGMEDEAKTPEQRRALAQVERDFRRAALRAERDAYYHMGRAGD
                     LPDDAVRKLVSEIDLLEERLNS"
BASE COUNT          213 a          446 c          550 g          301 t
ORIGIN      
        1 tgtttcggtt ttcggatggt tcagctttcg gacaggcggc gcaggatgtc ggcggcggcg
       61 gccagtctgg cgcgttcctc cggcgtcagg cgttcgatct ggcgcgtcag ccagtcctga
      121 cgcgcggcgc ggccttgagt cagccgttcg cggcaggcgg gggtgagcga aatcagggtc
      181 tggcgcccgt cgtccgggtc ggggcggccc tgagtcaggc ccgcggcctc cagcgcggcg
      241 acggtggcgc ccatcgattg cgggcgcacg ccctcggcgc gggccagggc cgagatggtc
      301 gccgggccgt ctgcgtccag ccgccgcacc acggcgatct gcgacggcgt gaggtcgccg
      361 acgctgcctt gctcgcgcag gcggcgtttc agccgtccga cgagggcgcg caggtccgcc
      421 gccagattgg cgatgtcgag ggagtccgtc atggggcgag tatgacggat atgaaggtaa
      481 actgtaaagt taaattgtga agtttggctt catatttttg tgtgggaaag ggaggggggc
      541 tttgtgaagg cccccctccg tcatggctgc gcaatgcccc tcgtttgcga aagctgtact
      601 ttcgctgcgc tcgacccacc gtgaacgggg gaggcgaagg gagcgattca ggaggtcttg
      661 gcggcgtctt ttttggcgga gcagtcgatt tcggcgcaga cgcgtttttg cgtttcggcc
      721 atcatgtcgg gcatgaagcc ggccactttc ggcatgaccc tggccatgac ctgcggcgtc
      781 ttggccagca tcgactggcc gaccgggctt tcgtagaagt tatccatcgc ggtcagctcc
      841 tcggtcgtga acacctcggc ataggcctgc gcggtggcgt cgagaatcct gggcatatag
      901 tcctgatcga cctgtatcac gatcgtcatc gccttctgca tcttggcgga atcggcgccg
      961 gtcagttcgg ggtgcgcctt tttcaggccc tcgatcaggg cgggcgtcat ctgctgcatc
     1021 acgccctgca tcatggcgtc gatgtgcatg gcgccgaaca tgcgatgcac gagggccagc
     1081 ttttccgggg tggcggcttc gggggcggga tcggcggcgg ccggagttgc ggccggagtt
     1141 gcagattgag cggcggccat atgcggcgcg gccatgaggc acagggccgc ggcggcggcg
     1201 gtgaggaacg gtttgcgcat ggaatctccc ccggagcgtt gcaggccgga ctcaagagcg
     1261 gtccggtgtg gagagcctag ccctattctt tgcaactgga aagtgaatta cgaattcagg
     1321 cgctcctcca gcaggtcgat ctcgctgacc agcttgcgca ccgcgtcgtc gggcaggtct
     1381 ccggcgcggc ccatatggta ataggcgtcg cgttcggcgc gcagggcggc gcggcggaag
     1441 tcgcgctcga cctgcgccag cgcgcggcgc tgttcggggg ttttggcctc gtcctccatg
     1501 cccttgagcc
//
LOCUS       DASVLL010000545         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_146827,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000545 DASVLL010000000
VERSION     DASVLL010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146827"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1664
                     /locus_tag="VF402_09010"
     CDS             372..1664
                     /locus_tag="VF402_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEX7800475.1"
                     /translation="MPATTSAAAAPKKTPWGAVLLFCMLFFIFGFVTWLNGPLITFVK
                     LAFNLTDVEAFLVPSVFYLSYFFLALPSAFILRKTGMKQGMALGLLIMAVGAAGFGQF
                     ATMRIFPGALVSLFGIGMGVSLLQTASNPYISVLGPIESGAQRIAIMGLFNKGAGFIA
                     PIVVGTLILYGMKDFADQVAAAPNPAAREALLNAFAARIHTPYLFMAGLLACLAVFTW
                     FSPLPALGDDANPDSGKGSRFAYIQMFFGFLAIFFYVGVEVLVGDGITTYANGFNLPL
                     DQTKFFTSGPLVGMILGYIVGLIAIPRFVSQEKYLRWSAILGIALAACAFLTKGYVSV
                     GFVAAMGFSNAMMWPAIFPLGIRGLGRLTEFGSALLVMGIAGGSIIPQLFAHLKDAYG
                     FQFVYAVLAIACYAYVLFFSLFAGRAGKPANTQANTQA"
     gene            1701..2609
                     /locus_tag="VF402_09015"
     CDS             1701..2609
                     /locus_tag="VF402_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7800476.1"
                     /translation="MKNPDRQARLFAGVDGGGTKCRVRLRDAEGQLLAQAEGGPANIR
                     LGLNMVWANILDALSHALQQAGRSDAYEHIAIGLGLAGIADADDVDRTLRAGPRFAHC
                     DAASDAHTACLGAFSGRDGAILISGTGSAGYAWVGAKAHPVGGWGFEVCDYGSAADLG
                     REAIRIALQAVDGLAPKSAFTEAMLRHFGGHPADIVHWVTTAKPRDYGALAPMIMGFA
                     NQNDIVAAPLVEKQAHDIGRYIARLLELGAGKVCLVGGMAPVFAPWLSSAARSALAEP
                     EQDALDGAILMARGAPNGLTSERADA"
     gene            2606..>2902
                     /locus_tag="VF402_09020"
     CDS             2606..>2902
                     /locus_tag="VF402_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEX7800477.1"
                     /translation="MRPLLEICVDTPAGLRAAAANGADRIELCAALSEGGLTPSRGFM
                     QRARGGGVPVRAMIRPRAGDFTYDDAELAVMRDDIAAAAEAGLAGVVFGANLADG"
BASE COUNT          462 a         1004 c          890 g          546 t
ORIGIN      
        1 ttcgtgccgc ctgcggcgaa ggcgagcccc tgaaacctta tgtctgatcg ccgcgtcgat
       61 aagtggtgga tcggcgggcc cgcatgccag tttgatacct gtacatacca acctaatacc
      121 taagctggga aaagagctaa ctttgaccct gatatagtgt caacttaaaa ctgccatatc
      181 tgcaattggt ctttaattgc gccgaatgcg tgacattaat atcgcagtta ttgtcatata
      241 ttttcctgaa aatagcgtgg gttacggaaa aacgccgaaa tcgcgcggga aatttcacgc
      301 gggagattgc aatttggccg aaactgagac ataccacttt tataaattgg tattataagg
      361 ggaaacaaac aatgcccgcc acgacatccg ccgcagccgc gcccaaaaag acgccatggg
      421 gcgccgtgct tctgttctgc atgctgttct tcatcttcgg cttcgtgacg tggctgaacg
      481 ggccgctgat caccttcgtc aagctggcct tcaacctgac ggacgtcgaa gccttcctgg
      541 tgccgtcggt cttttacctc tcctacttct tcctggccct gccctcggcc ttcatcctgc
      601 gcaagaccgg catgaagcag ggcatggcgc tgggccttct gatcatggcc gtcggcgcgg
      661 caggcttcgg tcagttcgcc accatgcgca tcttccccgg cgctctggtg tcgctgttcg
      721 gcatcggcat gggcgtgtct ttgctgcaaa ccgcctccaa cccgtacatc tcggtcctcg
      781 gcccgatcga atcgggcgcg cagcgcatcg cgatcatggg cctgttcaac aagggcgccg
      841 gcttcatcgc cccgatcgtg gtcggcacgc tgattctgta cggcatgaag gatttcgccg
      901 atcaggtcgc cgccgcgccg aaccccgccg ccagggaagc cctgctcaac gccttcgccg
      961 cgcgcatcca tacgccctac ctgttcatgg cgggcctgct ggcgtgcctg gcggtcttca
     1021 cctggttctc gccgctgccg gctttgggcg acgacgccaa tcccgattcg ggcaagggtt
     1081 cgcgcttcgc ctatatccag atgtttttcg gcttcctcgc catcttcttc tatgtcgggg
     1141 tcgaggtgct ggtcggcgac ggcatcacca cctacgccaa cggcttcaac ctgccgctgg
     1201 accagaccaa gttcttcacc tccggcccgc tggtcggcat gatcctcggc tacatcgtcg
     1261 gcctgatcgc cattccgcgc ttcgtcagcc aggaaaagta cctgcgctgg tccgccattc
     1321 tcggcatcgc gctggcggcc tgcgccttcc tgaccaaggg ctatgtcagc gtcggcttcg
     1381 tggcggcgat gggcttctcc aacgcgatga tgtggcccgc catcttcccg ctcggcatcc
     1441 gggggctggg caggctgacc gagttcggct cggccctgct ggtcatgggc atagcgggcg
     1501 gctcgatcat cccgcaactg ttcgcgcacc tgaaggacgc ctacggcttc cagttcgtgt
     1561 acgccgtcct cgccatcgcc tgctacgcct atgtgctgtt cttcagcctg ttcgccgggc
     1621 gcgcgggcaa acccgccaac acacaggcca acactcaggc ttgaacccgg taaagtcgct
     1681 tatctgctat gatcgttttc atgaaaaacc cggatcgtca ggcgcgtctg ttcgccggcg
     1741 tcgacggcgg cggcaccaag tgccgcgtgc gcctgcgcga cgccgagggc cagttgctgg
     1801 cgcaggccga gggcggcccg gccaatatcc gcctcggcct caacatggtc tgggccaata
     1861 tcctcgacgc cctgagccac gccctgcaac aggccggacg cagcgacgcc tatgagcata
     1921 tcgccatcgg cctggggctg gcgggcatcg ccgacgccga cgacgtcgac cgcacgctca
     1981 gggccgggcc gcgcttcgcc cattgcgacg ccgcgtcgga cgcgcacacc gcctgcctcg
     2041 gcgccttttc gggccgcgac ggcgccatcc tgatctccgg caccggcagc gcgggctatg
     2101 cgtgggtcgg cgcaaaggcg catccggtcg gcggctgggg cttcgaggtc tgcgactacg
     2161 gctccgccgc cgatctcggc cgcgaagcca tccgcatcgc cttgcaggcc gtcgacgggc
     2221 tggcgccgaa aagcgccttc accgaggcca tgctgcgcca tttcggcggc catccggcgg
     2281 acatcgtgca ttgggtcacc accgccaagc cgcgcgatta cggcgcgctg gcccccatga
     2341 tcatgggctt cgccaaccag aacgacatcg tggccgcgcc tctggtcgaa aagcaggcgc
     2401 acgacatcgg ccgctacatc gcccgcctgc tggagctggg cgcgggcaag gtctgcctcg
     2461 tcggcggcat ggcgccggtg ttcgcgccgt ggctgtcgtc cgccgcccgc agcgcgctgg
     2521 ccgaaccgga gcaggacgcg ctggacggcg ccatcctgat ggcccgcggc gcgccgaacg
     2581 gcctgaccag cgaaagggcg gacgcatgag gccgctgctg gaaatctgcg tcgacacgcc
     2641 cgccggcctg cgcgccgccg ccgccaacgg cgccgaccgc atcgaactgt gcgcggccct
     2701 gagcgaaggc ggcctgacgc cgtcgcgcgg cttcatgcaa cgggcgcgcg gcggcggcgt
     2761 cccggtgcgc gccatgatcc gcccgcgcgc cggggacttc acctacgacg acgccgaact
     2821 ggccgtcatg cgcgacgata tcgccgccgc cgccgaagcc ggattggcgg gcgtggtctt
     2881 cggcgccaat ctggccgacg gc
//
LOCUS       DASVLL010000546         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_198109,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000546 DASVLL010000000
VERSION     DASVLL010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198109"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VF402_09025"
     CDS             complement(<1..1332)
                     /locus_tag="VF402_09025"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7800478.1"
                     /translation="MSVGIAGAAALAGMVLAGSAGAQARKTSGDKPSGAAPTQVTVEG
                     KRKEASDRIDRRVYDVSHDPDAATGTGADILNKLPSVQVAPSGAVSLRGDSRVVVLVD
                     GKYPANGNAAIQNMPASGIERIEVMTTPSAQYAADAPGGIINIITRKRAHLGVSGEVS
                     TRLSNLGDNLHAAVTASKGPWTLDTRLVYAHGDKTWRQIDTLTAPYAMTDEGRYGWAQ
                     DAAVATANLTYKLDDARSLAFDSEARTMWAQLHNDDRYRLDQADYDQAGRGRIRDQYA
                     SVELIYEDNNTQTGRRLTLDMWRVDYDNLTRNAYRDDYRSAPGAAVYGQHIRRRGPED
                     DVKLDYERRMDNGDQLTAGAEWERIAADIDTIYSDSGAIAGPYPDGLTQRFSGARATL
                     DAYATYQHTLGAWTVLPGLRLEQERVEVADGGVASDFRHVLPSLHLSRALGK"
BASE COUNT          243 a          564 c          496 g          315 t
ORIGIN      
        1 cttgcccagc gcgcgcgaca ggtgcagcga cggcagcacg tgacggaaat cggacgcgac
       61 gccgccgtcc gcgacctcga cgcgctcctg ctcaaggcgc agccccggca ggacggtcca
      121 ggcgccgagc gtatgctgat aggtcgcata ggcgtccagc gtggcgcgcg cgccgctgaa
      181 cctctgcgtc aggccgtccg gatacggtcc cgcgatcgca ccgctgtcgc tgtagatcgt
      241 gtcgatatcg gccgcgatcc gctcccattc cgctccggcg gtcagttgat cgccattgtc
      301 catgcgccgc tcatagtcga gcttgacgtc gtcctccggt ccccggcggc ggatatgctg
      361 gccatagacc gccgcgccgg gcgccgaccg gtaatcgtcg cgataggcgt tgcgcgtcag
      421 gttgtcgtaa tcgacgcgcc acatatccag agtgagacgc cgccccgtct gcgtattatt
      481 gtcttcatag atcagttcga cggaagcgta ctgatcgcga atgcgcccgc gtccggcctg
      541 atcgtaatcg gcttggtcga ggcgatagcg gtcgtcgttg tgcagttgcg cccacatcgt
      601 ccgcgcctcg ctgtcgaagg ccaggctgcg cgcatcgtct aacttgtagg tcagattggc
      661 ggtggcgacg gcggcgtcct gcgcccagcc atagcgccct tcgtccgtca tcgcataggg
      721 cgccgtcaaa gtgtcgatct gccgccaggt cttgtcgccg tgggcgtaaa ccagccttgt
      781 gtccagcgtc cacggcccct tcgacgccgt caccgcggcg tgcagattgt cgccgagatt
      841 gctgaggcgc gtcgagactt cgccgctcac gcccagatgc gcgcgcttgc gggtgatgat
      901 gttgatgatc ccgcccggcg cgtcggcggc gtattgcgcc gatggcgtgg tcatgacctc
      961 gatgcgctcg atgccggacg cgggcatgtt ctgaatcgcc gcattgccgt tggccggata
     1021 tttgccgtcc accaatacga ccacgcgcga gtccccgcgc agcgacaccg cgcccgacgg
     1081 cgcgacctgc accgagggca atttgttcag aatatcggcg cccgtgcccg tcgcggcgtc
     1141 gggatcgtgc gatacatcat agacgcgccg gtcgatgcgg tcgctggcct ccttgcgctt
     1201 gccctcgacg gtcacctgcg tcggcgccgc gccggacggc ttgtcgccgg atgtttttct
     1261 cgcctgcgcc cccgcgctcc ccgccagaac catgccggcc agcgccgccg cacccgcgat
     1321 tccgacgctc atccccctcg atgtcacctt acccccgcga tcatatacct gaacgctgat
     1381 actatactcg ttacaattgc aagcaccaga ggcaagttcg ggtattttta cggttacctc
     1441 tccctcaagg ccgttcgcgc gccgtgcttg cctttttccg tccgatcctg taaagggcgc
     1501 gcttccctgc cgggcggtcg gctcgcatcg tgcgaaccac cgatccatca tggccctgag
     1561 acttccgctc aaggccgtgc cgcccggcgc aacggaagag gataagatgc cttactac
//
LOCUS       DASVLL010000547         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_468497,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000547 DASVLL010000000
VERSION     DASVLL010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468497"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..2556
                     /locus_tag="VF402_09030"
     CDS             283..2556
                     /locus_tag="VF402_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800479.1"
                     /translation="MNSSRLSSFARYGVTGLYGLMLLGAVAAPAFAQDTSQTAAPQKA
                     PQKDGQKAGKSDAPTTVTVVGQKPPVQNRIDRQVYDNTQNIDSTSGTASDALNKVPSV
                     NVDADGNVSLRGNSNVTVLMDGKPSTMLQGDNRAATLQSMSSNDIDSIEVMTNPGAQF
                     SAEGTGGIINLVMKKNRKPGTTGAVIANVGSQDRYNGALSIARNSGKLTLSGGLNVRH
                     DNRNSRVTSVLNRLDDNGDVVSGQTQTGTSLTTMDSVSANGAVSYNISDIDSVGAQLS
                     YARREFDADGASRYALTGAPPSGGLGDYTRTTDSRGPREDKTVDLNWNHTGDQPGETL
                     KADLRVSSSNGKSGSNNVNLYDGGETTYDTKLSSSRATDGDFSVDYTRNVGASGYLTA
                     GTDIRYDANHFLNVATGPDDAGDAATLNALLSSEFAYTQTISAAYATYQTNLTPKLVV
                     QGGLRVENQDLTTRRIDAGVGSSLNETKFSPSFFAAYSLTDKGKIRFSYSHRLQRPNP
                     QDLNPYVTYVDAQNVSQGNPDLKPQETDSFELGYEYSGGKINYQLRGYYRENQHTITD
                     YSYFLDDGVLLTTKRNFGSGNSGGVEFNVQGQLTPRLKVQLNSNWSQDELNTGALAGK
                     QTGSSVRGRVNLDYQVTKTDRLQLMYFTSGKQLTGQGYRAPFTGGNLSYRHNFNPKVA
                     LVASVNDPFRTAKFKMVTDNGKVYSESSRSMQAPTFYIGLTYLLGGAGANSDQNQWGG
                     RRWGGPGGGPGGHGGPM"
     gene            2677..>4362
                     /locus_tag="VF402_09035"
     CDS             2677..>4362
                     /locus_tag="VF402_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7800480.1"
                     /translation="MAGLSSSRAGWAGAFSPARAGWYAFILGLVFILIGVILAVGGVW
                     LIALGGNWYYALAGIALIVSGALLCSGRALGVWVYLGLFVVTVIWALLEVGLDGWGLL
                     PRVFAPFVLLVLAVPTLNRLEPRAPKLVWRGYGVLAVFAVILCAGIYLHTRHRVLRPV
                     PAAQGSFVYDSLNTGDKADWTSYGGGPAQQRYSDLNQINVSNVKNLRRAWIYHTGEIP
                     KNYGAETTPLKVGDRLYACTGMNRLFALDARTGKQVWSYNPGVDKEHIPYTAACRGVS
                     YYQVPGAAPGQACAARIFEGTLDGRVIAVDAADGKPCADFGRNGQVSITEGMGKVIPG
                     MVAITSPPVIVQGVVVTGHQVLDGQYRWAPSGAIHGFDAVTGQLRFVWDVMQPDLPAA
                     PPPGTQLTPGTPNMWTTATGDEQLGLVYLPMGNSAADYYSSARRPLEKQVSSALVALD
                     VHTGKPRWVFQTVHNDVWDYDLGSQVSLIDYPTAGGMAPALVLPSKTGEIYILDRRTG
                     KPLTATAQVRAPSGGVEPSERVPFQPVSGFATVRKANLKETDMWGMSPIDQMIC"
BASE COUNT          786 a         1447 c         1409 g          720 t
ORIGIN      
        1 ggggccgcgt ctttttttgc agcgcagcga cggaaagccc ggacaggcgc gtttgctctt
       61 tcggcgctcc gaagatccgc aaccatgagg cgcataatgt tgcggatatt ccgccaccgg
      121 tgtcgcagct tggccgcaat gtcgtggcat aggcggcgac gccacgccgc cggatgttca
      181 gatcccgttc ataatgccat ccttaacaag gcataatggc gaatttttga atttccggtt
      241 gaggttatga cgacaaaatc ttcttttaac aggctttaca gcatgaatag ctcgcgtttg
      301 tcttcttttg cccgttacgg cgtgacgggt ctgtacggcc tgatgctgct gggcgccgtc
      361 gccgcgcccg ccttcgcaca ggacacgtcc cagacggcgg ctccgcaaaa ggccccgcaa
      421 aaagacggcc agaaggccgg caagagcgat gcgcccacga ccgtgaccgt cgtgggccag
      481 aagccgccgg tgcagaaccg tatcgaccgt caggtctatg acaacaccca gaacatcgac
      541 tcgacctccg gcacggcgtc ggacgcgctg aacaaggtgc cgtcggtcaa tgtcgacgcc
      601 gacggcaatg tgtccctgcg cggcaattcc aacgtcaccg tgctgatgga cggcaagccc
      661 tcgaccatgt tgcagggcga caaccgcgcc gccaccttgc agtccatgtc gtcgaacgac
      721 atcgactcca tcgaggtcat gaccaatccg ggcgcgcagt tcagcgccga aggcacgggc
      781 ggcatcatca atctggtgat gaagaagaac cgcaagccgg gcaccacggg cgcggtcatc
      841 gccaatgtcg gctcgcagga ccgctataac ggcgctttgt cgatcgcgcg caattcgggc
      901 aagctgaccc tgtcgggcgg gctgaacgtg cgccacgaca accgcaattc gcgcgtgacc
      961 tcggtgctga accgcctcga cgacaatggc gacgtggtca gcggccagac ccagaccggg
     1021 accagcctga ccacgatgga cagcgtctcg gccaacggcg ccgtcagcta caacatcagc
     1081 gacatcgact cggtcggcgc gcaactgtcc tatgcgcggc gcgagttcga cgccgacggc
     1141 gcgtcgcgct acgccctgac cggcgcgccg ccgtcgggcg gcctcggcga ctatacgcgc
     1201 acgaccgaca gccgggggcc gcgcgaggac aagacggtcg acctgaactg gaaccacacc
     1261 ggcgaccagc ccggcgagac gctgaaggcc gacctgcgcg tcagctcatc gaacggcaag
     1321 tcgggctcga acaacgtcaa cctctatgac ggcggcgaga cgacctacga taccaagctg
     1381 tcgtccagcc gcgccaccga tggcgatttc agcgtcgatt acacgcgcaa tgtcggcgcc
     1441 agcggctatc tgaccgccgg gacggacatc cgctacgacg ccaaccactt cctcaacgtc
     1501 gccaccggcc cggacgatgc aggcgatgcg gcgacgctga acgcgctgct gtcgagcgag
     1561 ttcgcctata cgcagacgat cagcgcggcc tacgccacct accagaccaa cctgacgccc
     1621 aagctggtcg tgcagggcgg gctgcgggtc gagaatcagg atctgaccac gcgccgcatc
     1681 gacgcgggcg tcggctccag cctgaacgag acgaagttca gccccagctt cttcgccgcc
     1741 tacagcctga cggacaaggg caagatccgc ttctcgtact cgcaccggct gcaacgcccc
     1801 aatccgcagg atctgaaccc ctacgtcacc tatgtcgacg cgcagaacgt cagccagggc
     1861 aatcccgacc tcaagccgca ggaaacggat tcgttcgagc tgggctacga atacagcggc
     1921 ggcaagatca attaccagtt gcgcggctat taccgcgaga accagcacac catcaccgac
     1981 tattcctact tcctcgacga cggcgtgctg ctgaccacca agcgcaactt cggcagcggc
     2041 aattccggcg gcgtcgagtt caacgtgcag gggcaactga cgcccaggct gaaggtgcaa
     2101 ctgaacagca actggtcgca ggacgaactg aacaccggcg ccctggccgg caagcagacc
     2161 gggtcgtcgg tgcgcggccg cgtcaatctt gactaccagg tcaccaagac cgaccgcctg
     2221 caactgatgt acttcacctc gggcaagcaa ctgaccgggc agggttatcg cgcgccgttc
     2281 accggcggca acctgtccta tcgccacaac ttcaacccga aggtggcgct ggtcgcgtcg
     2341 gtcaacgatc cgttccgcac cgccaagttc aagatggtca ccgacaacgg caaggtttac
     2401 agcgaatcct cgcgctcgat gcaggcgccg accttctata tcggcctgac ctacctgctg
     2461 ggcggcgccg gcgccaacag cgatcagaac caatggggcg ggcggcgctg gggcggcccc
     2521 ggcggcggtc cgggcggtca cggcgggccg atgtaagcgc ccgccgcgta agagcttacc
     2581 gatattttat accagccggg gcaaatgccc cggctttttt gcgccttgaa gcctacggat
     2641 ggttgagtca ggtcataaac cggcggagga ttcgtcatgg ctgggttatc ttcgtctcgc
     2701 gcgggctggg ccggggcgtt ttcgcctgcg cgcgcgggct ggtacgcttt cattctcgga
     2761 ctcgtcttca ttctgatcgg cgtcatattg gccgtcggcg gcgtgtggct gatcgcgctg
     2821 ggcggcaatt ggtattacgc gctggccggg atcgcgctga tcgtatcggg cgcgctgctc
     2881 tgttcggggc gggccttggg cgtctgggtc tatctgggcc tgttcgtcgt gacggtgatc
     2941 tgggcgctgc tggaggtcgg gctggacggc tgggggctgc tgccgcgcgt gttcgcgccg
     3001 ttcgtgctgc tggttctggc cgtcccgacg ctgaaccggc tggagccgcg cgcgccgaag
     3061 ctggtgtggc gcggctatgg cgtgctggcc gttttcgcgg tcatcctgtg cgcgggcatc
     3121 tacctgcaca cgcggcaccg cgtcctcagg ccggtcccgg cggcgcaggg gtctttcgtt
     3181 tacgacagcc tgaacaccgg cgacaaggcg gactggacct cctatggcgg cggcccggcg
     3241 cagcagcgct attccgacct gaaccagatc aacgtctcga acgtgaagaa cctcaggcgc
     3301 gcctggatct accacaccgg cgagattccg aagaattacg gcgcggagac gacgccgctc
     3361 aaggtcggcg accggctcta cgcctgtacc ggcatgaacc ggctgttcgc gctggacgcg
     3421 cgcaccggca agcaggtctg gagctataat ccgggcgtcg acaaggagca tattccctat
     3481 acggcggcct gccgcggcgt cagctattat caggttccgg gcgccgcgcc ggggcaggcg
     3541 tgcgccgcgc gcatcttcga gggcacgctg gacggccgcg tcatcgcggt cgacgccgcc
     3601 gacggcaagc cgtgcgcgga tttcggccgg aacggtcagg tcagcatcac cgaaggcatg
     3661 ggcaaggtca tccccggcat ggtcgccatc acctcgccgc cggtgatcgt gcagggcgtg
     3721 gtcgtcaccg gccatcaggt gctggacggc cagtaccgct gggcgccgtc gggcgcgatc
     3781 cacggcttcg acgcggtgac gggccagttg cgcttcgtct gggacgtgat gcagccggac
     3841 ctgcccgccg cgccgccgcc ggggacgcaa ctgacgccgg gcacgccgaa catgtggacg
     3901 accgcgacgg gggacgagca actgggcctc gtctatctgc cgatgggcaa ttcggcggcg
     3961 gactattatt ccagcgcccg ccgcccgctg gaaaagcagg tctcctcggc cctggtcgcg
     4021 ctggacgtgc atacgggcaa gccgcgctgg gtgttccaga ccgtgcataa cgacgtctgg
     4081 gactacgacc tcggctcgca ggtcagcctg atcgactatc cgacggcggg cggcatggcc
     4141 ccggcgctgg tgctgccgtc caagacgggc gagatctaca tcctcgaccg ccgcaccggc
     4201 aagccgctga ccgccacggc gcaggtgcgc gcgccgtcgg gcggggtcga gccgtccgag
     4261 cgcgtcccgt tccagccggt gtcgggcttc gccaccgtgc gcaaggccaa tctgaaggaa
     4321 accgacatgt ggggcatgtc gccgatcgac cagatgatct gc
//
LOCUS       DASVLL010000548         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_306572,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000548 DASVLL010000000
VERSION     DASVLL010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306572"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF402_09040"
     CDS             complement(<1..236)
                     /locus_tag="VF402_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7800481.1"
                     /translation="MPRNRLYKNAPDPQASGVAEAPAAPFHLQLQVGSDGRVLIPAEL
                     RRQMRLEPGGMVNVELVDGELRLLSPAMALDRLER"
     gene            complement(288..1058)
                     /locus_tag="VF402_09045"
     CDS             complement(288..1058)
                     /locus_tag="VF402_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7800482.1"
                     /translation="MTDFLNQDPQWLDAPNGDRIAYRRIEGEGPPLLWLGGFLSDMTG
                     SKIAYLSDVCRRAGRAFWCFDYFAHGETGGDFARASVGRWRDNVLAVADRIDGPVTAI
                     GSSMGAHMLALLMTARPDKVRAAGFIAPAPDFATRLMLPSLSPEEKRRYEAFGMYMLP
                     GYDRPVPMSKAFFEDAARHEVLNAPIAFDGPVRILHGMKDEVVPWAHGVRLMETLTAR
                     DVRMELIRCGDHRLSRPQDLERLGEMAAELHGLALRTN"
     gene            1098..2276
                     /locus_tag="VF402_09050"
     CDS             1098..2276
                     /locus_tag="VF402_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7800483.1"
                     /translation="MSDPTFTACVLQVVPELDTGGVEQTVVDVSEAIVAAGGRSIVAS
                     KGGRLQARLEQGGAQVVNLPVHSKNPLTQWRVHRALRRLIRAHKADIVHVRSRAPAMA
                     AIAAARAEKVKSVATYHGIYNAKSALKRWYNRQMTRADLTIANSDYTREHILRAYPDV
                     APDRVIAIPRGVDLARFDPEGFDVRRVMKLEAAWGLPHAGDPGAPETRIRFLLAARLT
                     RWKGQALIVEAAARLRAAGRADFVILLAGDDQGRSDYTAGLKAAIAAHGLEDHVKLVG
                     HCADMPSAYQLCHYALCPSLEPEAFGRTAVEPQAMQRPPLAADHGATVETVVPGETGW
                     LVAPGDADAWAGAMRAAMDLDPETRFAMGAAGRARVKALFSLDAMCARTLDVYRRLLM
                     "
BASE COUNT          413 a          857 c          810 g          382 t
ORIGIN      
        1 cgctcaagcc tgtccagcgc catggcggga ctgagcaggc gcaattcgcc atcgaccagt
       61 tcgacattga ccattccgcc cggctccagg cgcatctggc ggcgcagttc ggcgggaatc
      121 agcacccgcc cgtcagagcc gacctgcaat tgcaggtgaa aaggcgcggc cggcgcttcg
      181 gcgacgccgg aagcctgcgg gtcgggcgca ttcttgtaca ggcggttgcg gggcatggcg
      241 gcgtctctgt caataacggc gttttatgcc atattgcatg aaaattgtca atttgtgcgc
      301 aaagccaggc catgcaactc cgccgccatt tcgcccaaac gctccaaatc ctgcggacgc
      361 gacaggcggt ggtcgccgca ccggatcagc tccatgcgca cgtcccgcgc ggtcagcgtc
      421 tccataagcc gcacgccatg cgcccacggc acgacctcat ccttcatgcc gtgcaggatg
      481 cgcaccggcc cgtcgaaggc gatgggcgcg ttcaacacct catgccgcgc ggcgtcctcg
      541 aaaaacgcct tcgacatcgg cacaggccgg tcatagccgg gcagcatgta catgccgaac
      601 gcctcatacc gccgcttttc ctccgggctc agcgacggca gcatcagcct ggtggcgaag
      661 tcgggcgcgg gcgcgatgaa accggcggcc ctgaccttgt ccggccgcgc cgtcatcagc
      721 agggccagca tgtgcgcgcc catgctggag ccgatggcgg tcaccggccc gtcgatcctg
      781 tcggccaccg ccagcacatt gtcgcgccag cggccgaccg aggcgcgagc gaagtcgccg
      841 cccgtctcgc cgtgcgcgaa atagtcgaag caccagaagg cgcggcctgc gcggcggcac
      901 acatcgctca ggtaagcgat cttcgagccg gtcatgtcgg acaggaagcc gcccagccac
      961 aacagcggcg gcccctcgcc ctcgatgcgg cggtaagcga tgcggtcgcc gttcggcgcg
     1021 tccagccatt gcgggtcttg atttaaaaaa tcggtcatgg tcaatggttt gccattgttt
     1081 cccgctatgc gcaattcatg tccgatccga ccttcaccgc ctgcgtgctg caagtcgtcc
     1141 ccgaactcga caccggcggc gtcgaacaga ccgtggtgga cgtctccgag gcgatcgtcg
     1201 cggcgggcgg gcgctcgatc gtcgccagca agggcggccg gttgcaggcg cggctggaac
     1261 agggcggcgc gcaggtggtc aacctgccgg tccacagcaa gaatccgctc acccaatggc
     1321 gggtccaccg cgccctgcgg cggctgatcc gcgcgcacaa ggccgacatc gtgcatgtgc
     1381 gctcgcgcgc cccggcgatg gccgccatcg ccgccgcgcg cgccgagaag gtgaagtccg
     1441 tcgccaccta tcacggcatc tataatgcga aatcggcgct caagcgctgg tacaaccgcc
     1501 agatgacgcg cgccgacctg accatcgcca attcggacta tacgcgcgag catatcctgc
     1561 gcgcctatcc cgacgtcgcg ccggatcggg tgatcgccat accgcgcggc gtcgatctgg
     1621 cgcgtttcga cccggagggt ttcgacgtgc gccgggtcat gaagctggaa gccgcgtggg
     1681 ggctgccgca cgcgggcgat ccgggcgcgc cggagacgcg catccgcttc ctgctggcgg
     1741 cgcggctgac gcgctggaag gggcaggcgc tgatcgtgga ggcggcggcg aggctgcgcg
     1801 ccgccggtcg cgcggatttc gtcatcctgc tggccgggga cgatcagggc cgcagcgact
     1861 acaccgccgg gctgaaggcc gccatcgcgg cgcacggcct cgaagaccac gtcaaactgg
     1921 tcggccattg cgccgacatg ccgagcgcgt accagctctg ccactatgcg ctgtgtccgt
     1981 cgctggagcc ggaagccttc gggcgcaccg ccgtcgagcc gcaggccatg cagcgcccgc
     2041 cgctggccgc cgatcacggc gcgacggtgg agacggtggt tccgggcgag acgggctggc
     2101 tggtcgcgcc cggcgacgcg gacgcctggg ccggcgccat gcgcgcggcg atggacctcg
     2161 atcccgaaac gcgattcgcg atgggcgcgg cgggccgggc gcgcgtcaag gccctgttca
     2221 gcctcgacgc catgtgcgcg cgcacgcttg acgtttaccg tcgattgctt atgtagacgg
     2281 cgcatgccgg acacacccgg ctgcaaatcc cgaaatttta acctttccgg cgcatagggg
     2341 gattgatccg gagcgcgagg aaagccataa tcttccgcgt ttcgacctac ccttcaaaca
     2401 gcctcaggag aacgacctat tcgtcgcccg attcaagccc cgcctcccaa agacggcccc
     2461 cg
//
LOCUS       DASVLL010000549         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_35980, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000549 DASVLL010000000
VERSION     DASVLL010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35980"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VF402_09055"
     CDS             <1..990
                     /locus_tag="VF402_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7800484.1"
                     /translation="TLDGLDSEGLFPSILGHEGAGVVLEVGPGVTSVKPGDHVIPLYT
                     PECRQCKSCLSRKTNLCTAIRATQGKGLMPDGTSRFSYKGQTIHHYMGCSTFSHYTVM
                     PEIAVAKIRDDAPFDKACYIGCGVTTGVGAVVNTAKVEPGANAIVFGLGGIGLNVIQG
                     LKMAGADRIVGVDLNDAKAEWGRRFGMTHFVNPAKVGDSKNGGDLVAHLVGLTDGGAD
                     YTFDCTGNTDVMRTALEACHRGWGESIIIGVAEAGREISTRPFQLVTGRVWKGSAFGG
                     ARGRTDVPKIVDWYMDGKIEIDPMITHTFALEDINKAFDLMHAGESIRSVVVF"
     gene            complement(1051..1503)
                     /locus_tag="VF402_09060"
     CDS             complement(1051..1503)
                     /locus_tag="VF402_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800485.1"
                     /translation="MKILPGAGAMLLLLTACSPAQDRDNGSTESAAPSMTAAEADSLM
                     ASEAGASLSDSLASAATLSGYVGQWRGPEGTGLTITPADGGYDVVIRNLDGPRDFRGD
                     LRDDGLHIERDGKALVIHPGDGDATGMKWLAGKQDCLVVDANEGYCRD"
BASE COUNT          305 a          578 c          564 g          296 t
ORIGIN      
        1 acgctggacg gactcgattc ggaaggactg ttcccgtcga tcctcggcca tgagggcgcg
       61 ggcgtggtgc tggaggtcgg ccccggcgtc accagcgtga agccgggcga tcacgtcatc
      121 ccgctctaca cgcccgaatg ccgccagtgc aaatcctgcc tgagccgcaa gaccaatctc
      181 tgcaccgcca tccgcgccac gcaaggcaag gggctgatgc cggacggcac gtcgcgcttt
      241 tcctacaagg gccaaacgat ccatcactat atgggctgct cgaccttcag ccattacacg
      301 gtcatgccgg agatcgcggt ggcgaagatc cgcgacgacg cgccgttcga caaggcgtgc
      361 tatatcggct gcggcgtgac cacgggcgtc ggggcggtgg tgaacacggc gaaggtcgag
      421 ccgggcgcca acgccatcgt cttcggcctc ggcggcatcg gcctcaacgt cattcagggg
      481 ctgaagatgg ccggggcgga ccggatcgtc ggcgtcgacc tcaacgacgc caaggccgaa
      541 tgggggcgcc gcttcggcat gacgcatttc gtcaacccgg cgaaagtggg cgatagcaag
      601 aatgggggcg atctggtcgc gcatctggtc ggactgacgg acggcggggc ggactacacg
      661 ttcgactgca ccggcaatac ggacgtgatg cgcacggcgc tggaagcctg ccatcgcggc
      721 tggggcgaga gcattattat cggcgtggcc gaggcgggcc gggaaatctc cacccgtccg
      781 ttccaactgg tgaccgggcg cgtctggaaa ggctcggcct tcggcggcgc gcgcggccgc
      841 accgacgtgc cgaagatcgt cgactggtac atggacggca agatcgagat cgacccgatg
      901 atcacccaca ccttcgcact ggaggacatc aataaggcgt tcgacctgat gcacgccggg
      961 gaaagcatcc gctcggtggt ggtgttctga cggcgaaaaa aggcccccgc cgccggcgcg
     1021 gacgggggcc tttcgccctc tcacaaggcg tcaatcgcgg caatagcctt cgttggcgtc
     1081 gacgaccaga caatcctgct tgcccgccag ccacttcatg ccggtcgcgt cgccgtcgcc
     1141 gggatggatg accagcgcct tgccgtcgcg ctcgatatgc agcccgtcgt cgcgcaggtc
     1201 gccgcgaaag tcgcgcggcc cgtccagatt gcggatcacg acgtcatagc cgccgtccgc
     1261 cggggtgatg gtcaggccgg tcccttcggg cccgcgccat tggccgacat agccgctgag
     1321 cgtcgccgcg ctggccaggc tgtccgacag gctggccccg gcctccgacg ccatcagcga
     1381 gtcggcctcg gccgcggtca tcgacggcgc cgcgctttcc gtggagccgt tatcgcggtc
     1441 ctgcgccggc gaacacgccg tcagaagcag caacatcgcg cctgcgcccg gcagaatctt
     1501 catcaaggcc tccgttacgg cgatgtcttt caccgccaga tttacgtcat ccttccgcac
     1561 aacaccatgc cgcttctatg gcgaagatat aaaatcgcgg ttagacttgg agcgcttttc
     1621 gagctgatgg gatcggatcg gcgctccaag cttttgtttt gccgctctta tccaaaaagt
     1681 ggaaccgcta tgacgccgca tattcgcctg ctcgccttcg gatcggccgg tcacgccgcc
     1741 gcc
//
LOCUS       DASVLL010000550         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_198285,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000550 DASVLL010000000
VERSION     DASVLL010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198285"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VF402_09065"
     CDS             <1..1205
                     /locus_tag="VF402_09065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7800486.1"
                     /translation="AGSLEAGLWAEADKAEKAARTSGERDGDPALEAYVSGVVSKVAG
                     PFAGDVRTYVMDRPFFNASMAPNGYTEVWTGLLLRCRTEDQLAFVVGHEAGHFRHSHS
                     LENFRRLKTSQNTALAASAVIAVVSLGAMANAPTYQSIRDIADMSRSLIDITYLSTMA
                     SLMRYSREREADADSYGLTYAAAAGYFTGGDADLWRNLIDENKASDFEKVRRRAARSS
                     IFDDHPLETDRIAALEAQDRRMNHGQPSTRSEADEKAARRAYRDRIRPYLGAWLKDDL
                     RRQDYGQTLYILNQLGQDGLDPGLLGFYTGEAYRLRDGAGADGVSDRDNAIRAYKAAL
                     TQPDAPADAWRALGDMYRRTGDVKDAVAAFTSYLAKAPGAADAWMVRDERDALIRDAA
                     APSTGGTS"
     gene            1202..1807
                     /locus_tag="VF402_09070"
     CDS             1202..1807
                     /locus_tag="VF402_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800487.1"
                     /translation="MIDRTLRFTAVIAFAGLLSACVSAPPLAPAGRLAATGDAHVTLD
                     RDWSDVTKLFAHRAVKTHILSLDGVLLNRLYVSEGLTPADPLMVNPARGDAPTAPAPR
                     GKADMSLSEQMEFVANSVAALDYQKVETSDPQPVSLGGAHGVRFALTARTGDGLNMRG
                     LAQAVPSKGKSYYIVYIAPAEHYYAASLKNATAVMDSETLP"
     gene            complement(1832..>2830)
                     /locus_tag="VF402_09075"
     CDS             complement(1832..>2830)
                     /locus_tag="VF402_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800488.1"
                     /translation="AEWHGFTMTDMVFPGFMVCVGLSMSLSLGRRMSEGGKGRMALHA
                     LRRAAVLVGIGLFLNLLPGFDFAHWRLPGVLQRIGVCYAIATGLLLLHSHRGTDGRLV
                     VHPRALAAWAAGLLIAYGVLLRYVPVPGYGAGHFDAVMSWPAWVDRAVFGPDHVWREA
                     KTYDPEGVLSTFPAVVNILIGLLVGLLIAAKTPARSVGQVALIGLLLTVAGLALDPVF
                     PIIKKIWTPSFVLLTCGIACLMLSALMLVMDRWGRKRWAMPIRVFGTNAILAYVFAWL
                     LAIALDVTGVYRVATEWLLGVVGDPYAMSLAFAVLVLLVCWLFVLPFYLKKIFLKV"
BASE COUNT          524 a         1018 c          893 g          395 t
ORIGIN      
        1 cggcgggcag tctcgaagcc gggctgtggg ccgaggccga caaggccgaa aaggccgcgc
       61 gcaccagcgg cgaacgcgac ggcgaccccg cgctggaagc ctatgtgtcc ggcgtggtga
      121 gcaaggtcgc cgggccgttc gcgggcgacg tgcgcaccta tgtcatggac cgtccgttct
      181 tcaacgccag catggcgccc aacggctata ccgaggtgtg gaccgggctt ttgctgcgct
      241 gccgcaccga ggaccagttg gccttcgtcg tcggacatga ggcggggcac ttccgccatt
      301 cgcattcgct tgaaaacttt cgacgcctca agaccagcca gaacacggct ttggcggcct
      361 cggcggtgat cgccgtcgta tcgctcggcg cgatggcgaa cgctccgacc tatcagtcta
      421 ttcgcgacat cgccgatatg tcgcgcagcc tgatcgatat cacctatctg agcacgatgg
      481 cgtccctgat gcgctattcg cgcgaacggg aggccgacgc cgattcctat ggtctgacct
      541 acgccgccgc cgccggatat ttcaccggcg gcgacgctga tctctggcga aatctgatcg
      601 acgagaacaa ggcgtcggat ttcgaaaagg tgcgccgccg cgccgctcgc agcagtattt
      661 tcgacgacca cccgctggaa acggaccgca tcgccgcgct ggaggcgcag gaccggcgca
      721 tgaaccacgg ccagccgtcc acacgctccg aagcggacga aaaggccgcg cgccgcgcct
      781 atcgcgaccg tatccgcccc tatctcggcg cgtggctgaa ggacgacctg cgccgtcagg
      841 actacggcca gacgctctat atcctgaacc agctcggtca ggacggcctc gatccgggcc
      901 tgctcggctt ctatacgggc gaagcctatc gcctgcgcga cggcgccggg gccgacggcg
      961 tctccgaccg cgacaacgcc atccgcgcct acaaggccgc gctgacgcag cccgacgcgc
     1021 ccgccgacgc ctggcgcgcg ctcggcgaca tgtaccgccg caccggcgac gtcaaggacg
     1081 ccgtcgccgc ctttacgtcc tatctggcca aggcgcccgg cgccgccgac gcctggatgg
     1141 tcagggacga acgcgacgcg ctgatccgcg acgccgccgc gccttccaca gggggaacct
     1201 catgatcgac cgcacgcttc gcttcaccgc cgtcatcgcc ttcgccggtc tcttgagcgc
     1261 ctgcgtctcc gcgccgccgc tggcgccggc gggcaggctg gccgcgaccg gcgacgccca
     1321 tgtcacgctg gaccgcgact ggtcggacgt gaccaagctg ttcgcgcacc gcgccgttaa
     1381 gacgcacatc ctgtcgctgg acggggtgtt gctgaaccgg ctttacgtgt ccgaggggct
     1441 gacgcccgcc gatcccttga tggtcaaccc ggcgcggggc gacgcgccga ccgcgcccgc
     1501 gccgcgcggc aaggccgaca tgtcgctgag cgaacagatg gagttcgtcg ccaattccgt
     1561 cgccgcgctc gattatcaga aggtcgagac gtccgatccg cagcccgtaa gcctcggcgg
     1621 cgcgcacggc gtgcgcttcg ccctcaccgc gcgcaccggc gacgggctga acatgcgcgg
     1681 gctggctcag gccgtgccgt ccaagggcaa gagctattac atcgtctata tcgcgcccgc
     1741 cgagcactat tacgcggcca gcctgaagaa cgccacggcg gtcatggaca gcgagacgct
     1801 accctaaaac tttggcctaa accttgggcc cctaaacctt gagaaatatc ttcttcaggt
     1861 agaagggcag cacgaacagc cagcagacga gcagcaccag cacggcgaag gccagcgaca
     1921 tggcgtaggg atcgccgacg acgcccagca gccactcggt cgccacgcga tagacgccgg
     1981 tgacgtccag cgcgatggcc agcaaccacg cgaacacgta ggccagaatg gcgttcgtgc
     2041 cgaagacgcg gatcggcatg gcccagcgct tacggcccca ccggtccatg accagcatca
     2101 gcgccgacaa catcagacag gctatgccgc aggtcagaag gacgaagctg ggcgtccata
     2161 tcttcttgat gatcggaaac accgggtcca gcgccagccc cgccaccgtc agcagcaggc
     2221 cgatcagcgc gacctgcccg accgacctgg cgggcgtctt cgccgcgatc agcagcccga
     2281 ccagcaggcc gatcaggata ttgaccaccg ccgggaaggt cgacagcacg ccttcgggat
     2341 cgtaggtctt cgcctcgcgc cagacatggt ccgggccgaa caccgcgcgg tcgacccagg
     2401 cgggccagct catcaccgcg tcgaaatgac cggcgccgta gccgggaacc ggcacgtaac
     2461 gcagcagcac gccataggcg atcagcagcc ccgccgccca tgccgccagc gcgcgcggat
     2521 gcacgaccag cctgccgtcc gtcccccgat gcgaatgcag cagcaaaagc cccgtggcga
     2581 tcgcgtagca gacgccgatg cgttgcagca cgcccggcag gcgccagtgg gcgaaatcga
     2641 agcccggcag caggttgaga aacaggccga tcccgaccag aaccgccgcc cgccgcagcg
     2701 cgtgcagcgc catccgcccc ttgccgcctt cggacatgcg ccgccccagc gacaggctca
     2761 tcgacaggcc gacgcagacc atgaagcccg gaaacaccat gtcggtcatg gtgaagccat
     2821 gccattcggc
//
LOCUS       DASVLL010000551         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_306618,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000551 DASVLL010000000
VERSION     DASVLL010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306618"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VF402_09080"
     CDS             <1..221
                     /locus_tag="VF402_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEX7800489.1"
                     /translation="YDSVIGMDKEEPLRRFTTRIGKERYKPASGEGTVCGVYVESDDR
                     TGLALRVEPIRVGGRLKAQIPQIGAYTA"
     gene            311..1069
                     /locus_tag="VF402_09085"
     CDS             311..1069
                     /locus_tag="VF402_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7800490.1"
                     /translation="MAGHSKFKNNMHKKGRADAARSKLFSKLSRDITIAAKMGLPDPA
                     MNSRLRLAVAMAKAESMPKDNIERAIKKGASGEADNMEEVRYEGRGPGGVGLIVEALT
                     DNRNRTAGNVRSYFTKFGGQMADTGSVSFMFDRLGEIRYPLSIGDADTVMEAAIEAGA
                     ADVETDDDAEEGGHVIYTAFEDLNEVTEALSKSLGDPKSTGFVWKPQALTPVTGDPVR
                     TLMKLIDAINDDDDVQNVFANFDISDEELEALGA"
     gene            1177..1557
                     /locus_tag="VF402_09090"
     CDS             1177..1557
                     /locus_tag="VF402_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800491.1"
                     /translation="MKASILMAAGLGTLALGLGGCAHYYPYPVDNSTQKQRAEACVAQ
                     YDPEHKAPQVMTYDSATGTETHMNTEGRKVSVTAQNAQDNTALGMGGKVGGDIPTACT
                     DYDPGVGPANPPAPHHKDSYNGYH"
     gene            complement(1747..>2984)
                     /locus_tag="VF402_09095"
     CDS             complement(1747..>2984)
                     /locus_tag="VF402_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7800492.1"
                     /translation="ATIRPCFGMLLDAQLRACGPQRHYREDGYRYGYYGSRADATVDC
                     DRAPPGYVEATARRIRPYGVLYLKARNRSCRASLDIDRSITIVGQGYGPNTIPVLVAP
                     DGENCIRISPNADKVILKNVYFSSPRGANTPCVEAAGKELTLQNSLIRYQGDNAAVHV
                     ANGRFNMTESSHIIAKTRGVALAVQQGEIYAENSEIASTAGGVYATLSGDSQLLGLSV
                     QQLADWHGFARGEDAVGLDIKLDSADSILTMNDLKVAYFARGIDLDGAGEALISHSLI
                     SHSDHAVTVSVARARIIENTLIANEIGVDVESGAAYVGRNQIANVRTAGILAQSDGEI
                     RAVDNAIDPNGEGCPTLRWGNIDPSQRVCRPWFKGSAFDVPGDAADQYMFDQFWPRMN
                     AAGYSASPDALAKPDGNRP"
BASE COUNT          549 a          986 c          965 g          484 t
ORIGIN      
        1 actatgacag cgtgatcggc atggacaagg aagaacccct gcgccgcttc accacccgca
       61 tcggcaagga gcgctataag cccgcgtccg gcgaaggcac ggtctgcggc gtctatgtcg
      121 aaagcgacga ccgcaccggc cttgccctgc gcgtcgagcc gatccgcgtc ggcgggcgtc
      181 tcaaggcgca aatcccgcag atcggcgcct acaccgcctg acgggcttca caaacagcct
      241 gcgccgggct agaaacccgc gatcattttc aaagacatgc cgccccggat gcgcggcgta
      301 agaggacgtc atggctggcc acagtaaatt caagaacaac atgcacaaga agggccgcgc
      361 cgacgccgcg cgctccaagc tcttctccaa gctgtcgcgc gacatcacca tcgccgccaa
      421 gatgggcctg cccgatccgg cgatgaattc gcgcctgcgc ctggccgtgg cgatggccaa
      481 ggccgaatcg atgcccaagg acaatatcga gcgcgccatc aagaagggcg cctcgggcga
      541 agcggacaat atggaggagg tccgctacga aggccgcggc cccggcggcg tcggccttat
      601 cgtcgaggcg ctgaccgaca accgcaaccg cacggcgggc aatgtgcgca gctatttcac
      661 caagttcggc ggccagatgg ccgacaccgg ctcggtcagc ttcatgttcg accggctggg
      721 cgagatccgg tatcccttgt cgatcggcga cgccgacacg gtcatggagg ccgccatcga
      781 ggcgggcgcc gccgatgtcg agacggacga cgatgcggag gagggcggcc acgtcatcta
      841 caccgcgttc gaggatctga acgaggtcac cgaagcgctg tcgaaatcgc tgggcgatcc
      901 gaaatcgacc ggcttcgtct ggaagccgca ggccctgacg ccggtgacgg gcgatccggt
      961 gcgcacgctg atgaagctga tcgacgcgat caacgacgac gacgacgtgc agaacgtctt
     1021 cgccaatttc gacatctcgg acgaggagct ggaggcgctg ggcgcctgac ccgccggacg
     1081 atttccggga tctttgccgc tttcattctg ggggctttgt ttacccccgg aaatgtgcaa
     1141 ttatgcgcgc gttaaccatt ttcaccgggg gagtgaatga aagcgtctat tctgatggcc
     1201 gccggattgg gaacgctggc gctgggcctt ggcggatgcg cgcactacta tccctatccc
     1261 gtcgacaaca gcacgcagaa gcagcgcgcc gaggcgtgcg tggcgcagta cgaccctgag
     1321 cacaaggcgc cgcaggtcat gacctacgac agcgccaccg gcaccgaaac ccacatgaat
     1381 accgaaggcc gcaaggtcag cgtcaccgcc cagaatgcgc aggacaacac cgcgctcggc
     1441 atgggcggca aggtcggcgg cgacatcccg accgcctgca ccgactacga ccccggcgtc
     1501 ggcccggcca atccgccggc gccgcatcat aaggatagct ataacggcta tcattgagga
     1561 cgcgtcggac gtccgcgctc aaccggcaga cagcccctca cccgatcgcc atcgcctgac
     1621 ggctcggctc tcgtcctctc ccctcgtgga ggggagaggg gaaggttgga gtcaccttct
     1681 cctccccatc ctcgatgggg aggtggcgtt tgcgcagcaa acgacggagt ggtattcctc
     1741 caccccctaa ggccggttgc cgtccggctt ggccaacgca tccggcgaag cgctgtaccc
     1801 cgccgcattc atcctcggcc agaactgatc gaacatatac tgatccgcgg cgtcgcccgg
     1861 aacgtcgaag gccgagccct tgaaccacgg gcggcagacg cgctgcgacg ggtcgatatt
     1921 gccccagcgc agggtgggac agccttcgcc gttcgggtcg atggcgttgt ccaccgcgcg
     1981 gatctcgccg tccgactgcg ccagaatccc ggcggtgcgc acattggcga tctggttgcg
     2041 cccgacatag gccgcgccgc tttcgacgtc gacgccgatc tcattggcga tgagggtgtt
     2101 ttcgatgatg cgcgcgcggg cgaccgacac ggtgacggca tggtcgctgt gcgagatcag
     2161 gctgtgcgag atcagcgcct cgcccgcgcc gtccagatcg atcccgcgcg cgaaataggc
     2221 caccttcagg tcgttcatcg tcaggatgga atcggcgctg tccagcttga tgtccagccc
     2281 gaccgcgtcc tcgccgcgcg cgaagccatg ccagtcggcc agttgctgca cgctcaggcc
     2341 cagaagctga gaatcgccgc tcagcgtggc gtagaccccg cccgccgtcg aggcgatctc
     2401 ggagttctcg gcgtagatct ccccctgctg cacggccagg gccacgccgc gcgttttggc
     2461 gatgatgtgc gacgattcgg tcatgttgaa gcggccgttc gccacatgca cggcggcgtt
     2521 gtcgccctga tagcggatca gggaattctg caaggtcagc tccttgcccg ccgcctcgac
     2581 gcacggcgta ttggcgccgc gcgggctgga aaaatagacg tttttcagga tcaccttgtc
     2641 ggcgttgggc gagatgcgga tgcagttctc gccgtcgggc gccaccagca ccggaatggt
     2701 gttcggccca tagccctgac cgacgatggt gatcgaacgg tcgatgtcga ggctggccct
     2761 gcacgagcgg ttgcgcgcct tcagatacag cacgccgtag ggccggatgc ggcgcgctgt
     2821 cgcctcgaca tagcccggcg gagcgcggtc gcaatcgacg gtcgcgtcgg cgcggctgcc
     2881 gtaataaccg tagcggtagc cgtcctcgcg gtaatggcgc tgcggaccgc acgcgcgcaa
     2941 ctgcgcgtcg agcagcatgc cgaagcacgg ccggatcgtg gcgt
//
LOCUS       DASVLL010000552         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_147087,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000552 DASVLL010000000
VERSION     DASVLL010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147087"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VF402_09100"
     CDS             complement(<1..340)
                     /locus_tag="VF402_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800493.1"
                     /translation="MSVLSNPLQRLKRKLDPAAVPVLVVEDHDASRRLVMELLRASGF
                     GNLYFARDAEAAIGLIAAHPPDLMIVDWNLPGMTGLELVESMRCAALTGDARFPNPQA
                     PIVMLTARQRA"
     gene            506..907
                     /locus_tag="VF402_09105"
     CDS             506..907
                     /locus_tag="VF402_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HEX7800494.1"
                     /translation="MNRRKLFALPVLTLAGLAAPVLAQAETKKKGGGEGYTQFPTINV
                     FTDAGRLRHGSLSVDVGLYSDNPRLVAQIKLYAPRLQDAYVTVLQAYAGDLSRTSLVD
                     TDYIATQLQTATDRVLGVKGARVLLGSILLN"
     gene            985..1344
                     /locus_tag="VF402_09110"
     CDS             985..1344
                     /locus_tag="VF402_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HEX7800495.1"
                     /translation="MTSSPSPFESGLKLMEAQSQLAARSLINLIEMISTSSHRYAEDT
                     GKFTREALELMNAAATTQDAVALAELQKRWAETCLKYGQDRTAATMHFVEQCGQQALN
                     IAARHAHPSNGMGAPKP"
     gene            1390..>2149
                     /locus_tag="VF402_09115"
     CDS             1390..>2149
                     /locus_tag="VF402_09115"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX7800496.1"
                     /translation="MSLSGKTALITGSTSGIGLGVAKGLAAAGANVMLNGFGEADAIE
                     AERAGLEKDYGVKARFNGADLTKPAEIEALIRACDELGGVDIIVNNAGIQHVAPVEEF
                     PPEKWDAIIALNLTASFHTVRLSFAAMKAKKWGRILNIASAHALVASPFKSAYVSAKH
                     GLLGFTRTVALEGAEHGVTCNAICPGYVHTPLVDKQIPDTMKARGLTREQVIRDVILA
                     AQPTKEFVTVEDIAALAVFLCSNAGKSINGAPLSI"
BASE COUNT          415 a          731 c          653 g          350 t
ORIGIN      
        1 gcgcgcgctg ccgcgcggtc agcatgacga tcggcgcctg cgggttggga aagcgcgcat
       61 cgccggtcag cgcggcgcag cgcatgcttt cgaccagttc gagtcccgtc atgcccggca
      121 aattccagtc gacgatcatc aggtcgggcg gatgcgcggc gatcagcccg atcgctgcct
      181 cggcgtcgcg cgcgaagtag agattgccga acccggaggc gcgcagaagc tccatgacca
      241 gacggcgcga cgcatcgtga tcctcgacga cgaggaccgg aaccgcggcc ggatcgagtt
      301 tgcgcttcag gcgctgaaga ggattcgaca acacgctcat cggatgcgaa agacagacaa
      361 ccccgcttgg gccagacctg gccgatccgt tcagactacc gccgaagccc ttaatgcgcc
      421 gttgatcaag acactgattt catagttaat ttttcattcg ccattataaa cgccgtctta
      481 acgcccctga gttcagagtt tcgccatgaa tcgccgcaaa ctgttcgccc tgcccgtatt
      541 gaccctggcc ggcctcgccg cgcccgttct ggcgcaggcc gagaccaaga agaagggcgg
      601 cggcgaaggc tacacgcaat ttccgaccat caacgtcttc accgacgccg ggcgcctgcg
      661 ccacggctcg ctcagcgtcg acgtcggcct ctacagcgac aaccccaggc tggtcgcgca
      721 gatcaagctg tacgcgccgc gccttcagga cgcctatgtc acggtgttgc aggcctatgc
      781 gggcgatttg tcgcgcacct cgctggttga caccgactac atcgccacgc aacttcagac
      841 ggcgaccgac cgcgtgctgg gcgtcaaggg cgcgcgcgtg ctgctcggct cgattttact
      901 gaattagcgt tgcggcgcgc gtgtttcggt catagtttcg acaggctggc cggataggtt
      961 tgcggcaatc tgcggaggca tcgcatgacg tcatcaccct ccccgttcga aagcggcctg
     1021 aagctgatgg aggcgcaaag ccagttggcg gcgcgcagcc tgatcaacct gatcgaaatg
     1081 atctcgacca gctcgcaccg ctatgccgag gacaccggca agttcacacg cgaggcgctg
     1141 gagctgatga acgccgccgc gacgacgcag gacgccgtcg ccctggccga gttgcaaaag
     1201 cgctgggccg agacgtgcct gaaatacggt caggaccgca ccgccgccac catgcatttc
     1261 gtcgagcaat gcggccagca ggcgctgaac atcgccgcgc gccacgccca tccgtcgaac
     1321 ggcatgggcg cgccgaagcc ctgatccccc gtttcctccc cccagtaaaa tacatcaagg
     1381 agcgttctca tgagcctgtc cggtaaaaca gcgctgatca ccggttcgac cagcggcatc
     1441 ggcctcggcg tcgccaaggg gctggccgcc gccggcgcca acgtcatgct caacggcttc
     1501 ggcgaggccg acgccatcga ggccgaacgc gcgggcctgg aaaaggatta cggcgtcaag
     1561 gcgcgcttca acggcgccga cctgaccaaa cccgccgaga tcgaggcgct gatccgcgcc
     1621 tgcgacgaac tgggcggggt cgacatcatc gtcaacaatg cgggcatcca gcacgtcgcg
     1681 ccggtcgagg aattcccgcc ggaaaaatgg gacgcgatca tcgcgctcaa cctgaccgcc
     1741 agcttccata cggtgcgcct gagcttcgcg gcgatgaagg cgaaaaaatg gggccgcatc
     1801 cttaatatcg cctcggcgca cgccctggtc gcctcgccgt tcaagtcggc ctacgtctcg
     1861 gccaagcacg gccttctggg cttcacccgc accgtggcgc tggaaggcgc cgaacacggc
     1921 gtcacctgca acgccatctg ccccggctat gtgcataccc cgctggtcga caagcagatt
     1981 cccgacacca tgaaggcgcg cggcctgacg cgcgagcagg taatccgcga cgtgatcctg
     2041 gccgcccagc cgaccaagga attcgtcacg gtcgaggaca tcgccgcctt ggccgtcttc
     2101 ctgtgctcca atgcgggcaa gtcgatcaac ggcgccccgc tcagcatcg
//
LOCUS       DASVLL010000553         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_251876,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000553 DASVLL010000000
VERSION     DASVLL010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251876"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..528)
                     /locus_tag="VF402_09120"
     CDS             complement(115..528)
                     /locus_tag="VF402_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800497.1"
                     /translation="MMSPLSLLIVVVVVLHAVAAVGWMTSAGFVARGGGVGAERAFAP
                     QMMHALFVLISGGFLWSRLHAGAAGLYEITLGAGALCAVAAAVIQGGMVGPAVRRLKA
                     AGEAAGLRKTMARGNAIASALLMLAFVCMIAARFL"
     gene            complement(652..1686)
                     /locus_tag="VF402_09125"
     CDS             complement(652..1686)
                     /locus_tag="VF402_09125"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX7800498.1"
                     /translation="MIAALIAAALNTAALNAASLTMPDMPLHDPFILADKANKTYYLY
                     TSNNPRMSGTCGLGTMAYESHDLEHWSAPKAVFALPKGTWADGGAWAPEVHEWRRRYY
                     LFTTFHNESAPITDPAPKPHRQPYRRGTALAVADTPEGPFHLVNNGEPIAPARLMTLD
                     GTLYVDPDGRPWLVYAHEWVQQTDGTIEALPLDDELKAAGEPVTLFRASDAKWVHGSR
                     KGPDDTVYVTDGPELYRTRDGHLLMLWSSYGDGGYIEAQARSLTGRIEGPWAQLGPLV
                     TGDSGHGMLFHSFDGKLMLVLHHPFDNARGRLFEVRDAGDRLEIVRERTDLDHDGDAR
                     HPLPKANCGR"
     gene            complement(1683..>1929)
                     /locus_tag="VF402_09130"
     CDS             complement(1683..>1929)
                     /locus_tag="VF402_09130"
                     /inference="COORDINATES: protein motif:HMM:NF028067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-fucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7800499.1"
                     /translation="TQFGEGSWRFTAKDGAIYAIALTWPGETATIAALGDRAGKVASV
                     ELIGGGNIAFTQAPDGLHLKLPRQPDEIAYAFRVRMR"
BASE COUNT          321 a          684 c          632 g          292 t
ORIGIN      
        1 gaccgcgcgc agcacgtccg ggccggggcc gtagccgaag gtcacggcct cgaaacggac
       61 ggcgggcgaa agcgtctgcg aggcggcgtg gagcgaatcc atgagaatct cccgttacaa
      121 aaagcgcgcc gcgatcatgc agacgaaggc cagcatcagc agggccgaag cgatcgcatt
      181 gccgcgcgcc atcgtcttgc gcagccccgc cgcttcgccc gccgccttca gccgccgcac
      241 ggcgggaccg accatgccgc cctgaatgac cgccgccgcc acggcgcaga gcgcgcccgc
      301 gccgagcgtg atttcataga gtccggcggc cccggcgtgc aggcggctcc acagaaagcc
      361 gccgctgatc aggacgaaaa gcgcgtgcat catctgcggc gcgaaggcgc gctcggcccc
      421 gacgccgccg ccgcgggcca cgaacccggc ggaggtcatc cagccgaccg ccgccacggc
      481 gtgcaggacg acgacgacga cgataagcag cgacaggggc gacatcatgc ggcggctccg
      541 gcaggggtga aagaacccgg ttacgtcgcg aacacataac cgaccgacgg tcggtcgtca
      601 agcgcgcaaa tccgcaaagc ggcgccccat gtcagcgcgt cgccatgccg gttatcgccc
      661 gcagttcgcc ttcggtaagg gatggcgcgc atcgccgtca tggtcgagat cggtgcgttc
      721 gcgcacgatc tccagccggt cgcccgcgtc gcgcacctcg aacagacgtc cgcgcgcatt
      781 gtcgaacgga tgatgcagca ccagcatcag cttgccatcg aagctgtgga acagcatgcc
      841 gtggccggaa tcgcccgtga ccagcgggcc gagctgcgcc cacggcccct cgatgcgccc
      901 ggtgagggag cgcgcctgcg cctcgatata accgccgtcg ccatagctgg accacagcat
      961 gagcagatgg ccgtcccgcg tgcggtaaag ctccggcccg tcggtgacat agaccgtgtc
     1021 gtccggcccc ttgcggctgc cgtgaaccca cttcgcgtcc gacgccctga acagcgtcac
     1081 cggctcgccc gccgccttca gttcgtcgtc cagcggcagc gcctcgatcg tcccgtccgt
     1141 ctgttgcacc cactcatgcg catagaccag ccacggcctg ccgtccgggt cgacgtacag
     1201 cgtgccgtcc agcgtcatca ggcgggcggg cgcgatgggt tcgccgttat tgacgagatg
     1261 aaacggccct tccggcgtat ccgcgaccgc cagcgccgtg ccgcgccggt aaggctggcg
     1321 atgcggcttc ggcgccgggt cggtgatcgg cgcggactcg ttatggaagg tggtgaacag
     1381 gtaatacctg cgccgccatt catgcacttc cggcgcccag gccccgccat cggcccacgt
     1441 ccccttcggc agggcgaaca cggccttcgg cgcgctccag tgttcgaggt catggctttc
     1501 ataggccatc gtccccagcc cgcacgtccc gctcatgcgc gggttgttcg aggtatagag
     1561 gtaataggtc ttgttcgcct tgtccgccag aatgaacgga tcgtgcagcg gcatgtccgg
     1621 catggtcaga ctggcggcgt tcagggcggc agtgttcagg gcggcggcga tcagggcggc
     1681 gatcatctca tcctcacacg gaacgcatag gcgatctcat cgggttggcg cggcagtttc
     1741 agatgcaggc cgtccggcgc ttgcgtgaag gcgatgttcc cgccgccgat cagctcgacc
     1801 gacgcgacct tgcccgcccg atcgcccaga gccgcaatcg tcgccgtctc gcccggccat
     1861 gtgagcgcga tggcgtagat cgcgccgtcc ttggccgtga accgccagct cccttcgccg
     1921 aactgcgtc
//
LOCUS       DASVLL010000554         9911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_36151, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000554 DASVLL010000000
VERSION     DASVLL010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36151"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VF402_09135"
     CDS             <1..222
                     /locus_tag="VF402_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7800500.1"
                     /translation="SLDLAYVAAGQFDGYWERGIKPWDMAAGLLMVHEAGGKFASIDS
                     DASPFETGTVICANLDLFPEFLEKVRLAK"
     gene            complement(223..924)
                     /locus_tag="VF402_09140"
     CDS             complement(223..924)
                     /locus_tag="VF402_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800501.1"
                     /translation="MRPVCLALIAAFALTSSAHAGQSILDRLRPTTDEIKLQGKKGHD
                     LFHPGYALGAYAGEIHAATRHAEGLGLFTSDKFKTGFTVTGPGLGDGVTADCAGGQGR
                     IGLDWLAFKRDELAYICSYGGAAPKDAELDLALSTGGKLLNRLAQPQRGGEFHYGEVT
                     LRVTTELLGGLTLSNGQTSDYLIARPDGTPVAAVRKTGLQPTVYLPKAPGPERDAAAV
                     LALSLIFFNDPGNTR"
     gene            complement(980..1624)
                     /gene="thiE"
                     /locus_tag="VF402_09145"
     CDS             complement(980..1624)
                     /gene="thiE"
                     /locus_tag="VF402_09145"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX7800502.1"
                     /translation="MTDCRLYLITPPRIDDADAFARALQAALTAGGETVAALQIRLKD
                     VDEAHIRAVTRVLTPIAHAQGAAVLMNDHAMLAKQLGLDGVHVGQSDTPYKEARRLLG
                     PDAMIGVTCHNSRHLAMEAAEAGADYVAFGAFFPTTTKTVEHMAELETLEIWQETMQT
                     PCVAIGGITAANARAVAEAGADFIAVSGAVWNCPDGAAAGVTALKKALRAAAQD"
     gene            1752..1937
                     /locus_tag="VF402_09150"
     CDS             1752..1937
                     /locus_tag="VF402_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7800503.1"
                     /translation="MYVCNCNGIRERDALQVIESGAATVRQVFDGCGARPQCARCVCD
                     IRDMLDEAGCAMAIAAE"
     gene            2015..2500
                     /gene="bfr"
                     /locus_tag="VF402_09155"
     CDS             2015..2500
                     /gene="bfr"
                     /locus_tag="VF402_09155"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX7800504.1"
                     /translation="MQGQPEIIKLLNKVLTNELTAVNQYFLHARMIENWGLYHLGRIV
                     YNESIEEMKHADLLIKRILFLDGLPNLQDLHKLRIGETIGECLASDLRVETGGRDTLV
                     AGVKLCEDSQDYVSRDLLARILSDTEHHIDFLETQLNLIRLMGEPNYIQSAMKEIEGE
                     A"
     gene            2605..3528
                     /locus_tag="VF402_09160"
     CDS             2605..3528
                     /locus_tag="VF402_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7800505.1"
                     /translation="MTLLGACALTGLALTGLAACGRPDGAFSDDRAPDDSDAGDDASE
                     TIDPRLAQQQGTLQWAVSGAWRNAVDKARDKYQHPIETLQFFEVDPKATVVDMWPGAG
                     YMTEVLAPYLNAGKGEYVAALIAPRPGDQAIQALNDAYRKHFDADKKLYGDITYSQFG
                     ADTGPLVPAGTADCVLMITVIQDWMASGVVEKAFADAFAALKPGGVLGVEQHRADIGN
                     VQDPAATNGYVQEPFVRQLAAEAGFAFVAASEINANPKDDKDHPFGVWTLPPQRLTAP
                     RGQPPNPDFDGTLYESIGESDRMTLKFRKPS"
     gene            3525..4586
                     /locus_tag="VF402_09165"
     CDS             3525..4586
                     /locus_tag="VF402_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7800506.1"
                     /translation="MSRIAALSTSAPLMFTPESRPPEGGVGEWLRGAGGLRLRVGYWP
                     APEGGTSEGASRGTVFISPGRSEPIEKYYELARDLIGRGFDVVAHDWRGQGLSARLLP
                     DRLKCHARSADEFLDDFQRLLDAFEERAPKPWIALGHSMGAALNMATLVKGEPRISGA
                     LLVNPMLRIKTGKHSLWSVNFQVNWQVNHGRGADYVPELFEDPFENTFETDALTHDPA
                     RYERWREQLFACPHLAVGSPTWGWLQFALRLGETLLRDKYKLLKKVRTPITFVCSGDD
                     HVIAHPPTRAFARKIAGAQYIEVAGAEHELLIEADEYRDQALRAFDELADFVAPRGGG
                     TEAGDAPVGEDAEAEEQAG"
     gene            complement(4669..5205)
                     /locus_tag="VF402_09170"
     CDS             complement(4669..5205)
                     /locus_tag="VF402_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800507.1"
                     /translation="MLEFGDLLNLVRDSYITQFSAFVDQQRAGHDNGGPEVKFELTED
                     SDFFGHAYCADYASPEDGGHVVELSPDEEISFDPLQVSFGEMEMAVTRLAWHDMTFSL
                     PQGAHVAPDRIAPWFETWFDPNEERFDPDTRFSGNIHALTLDADGMNVDFGTAPLDAF
                     IELVELLEAEGCKAVAIS"
     gene            complement(5304..6881)
                     /locus_tag="VF402_09175"
     CDS             complement(5304..6881)
                     /locus_tag="VF402_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7800508.1"
                     /translation="MFDPGKTDLNLPSGPGGRPSPRQGFYARRLAHAAAALGRLGFEA
                     GTRAFKAARDFAFSLSGQLLILTLVFVVIVEVMIVIPRLAADQEDWLTDRVRSAEIAS
                     QAVDTATANNVSRATAFQLLRSAGVLYLRLQGGDRYYMLNPLPDLQIPDEVLDMRDSH
                     KDLRHDLAYMWAPWETLMGKPDRLIHLIAKPRVRSGDMIEIVVRAAPLKTWLQSSLIG
                     MLRMSLSISLAAGILVFAALSAFIVRPIRKLTDGIAAFRTNPEDPSATPKLSGRRDEI
                     GHIERELASMQEQVRQALRSRARLAALGQAVSKINHDLRNMLTSAQMASDRLAASADP
                     AVAKALPRLERALDRALSLAQNVLTYGKSDERAPEIQIVRLRELADAAAEDAGLGVTP
                     QAAENVRFIARLPRGFCLEADPEQLHRLLVNLMRNARQAIELQPNRKTRGRVTLTAIK
                     TAQDVVLIVADNGPGIPERARDKLFQPFSSSATPGGSGLGLAISRELAQLHGGDVRLV
                     QTGPDGTTFEIRLPLKG"
     gene            7121..7261
                     /gene="rpmH"
                     /locus_tag="VF402_09180"
     CDS             7121..7261
                     /gene="rpmH"
                     /locus_tag="VF402_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7800509.1"
                     /translation="MGTKRTYQPSRLVRKRRHGYRARMATKNGQKVVARRRAKGRKRL
                     TA"
     gene            7349..7750
                     /gene="rnpA"
                     /locus_tag="VF402_09185"
     CDS             7349..7750
                     /gene="rnpA"
                     /locus_tag="VF402_09185"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX7800510.1"
                     /translation="MISRIKVRSDFVAASKAPWQARPGVVVQARPRCDDAAMRAGFTA
                     TKKVGNAVIRNRAKRRLRAAAEAMLPLYGQPGHDYVFVARAQTPARDWQALLDDVKAA
                     LLRLSRPQSQRDNRPGPVAAPSHAPQDKSAP"
     gene            7747..9585
                     /gene="yidC"
                     /locus_tag="VF402_09190"
     CDS             7747..9585
                     /gene="yidC"
                     /locus_tag="VF402_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX7800511.1"
                     /translation="MNKDDNNRNMIFFLIASMVLLAAYQYFVLSPQQKKQQAAQAAAA
                     ASSSSAAAAGLPMPGQPVVLTRPQALAQSPRVPIDTPSLKGSIALKGALLDDLYLAKY
                     HETVDPKSPLVELLSPAGTAHPYYAEAGYFGQNVPNLPGIGAQWTLVSGDKLAPGKPV
                     TLAYDNGAGLKFQRTYAIDDNYMITVTDSVANTGAQAVQLEPYASVTRVGMPVEVGRS
                     GYVMEGAMATVSKDAADAKAGYHSQSKRYKDLIKDKTPPQGQSMGGWYAVTDKYWMAS
                     VIPNQGQLVNYSLSATALPDKTPVFRAGYSDAAVTVAPGATWTSQSHIFAGAKLHDQL
                     NAYQKSLNLPRFGYALDWGHLDWLTIPFYLLLVQLYKWIGNFGFAILALTVVIKIIFY
                     PLAHRSYVAMMKMKHVQQHLAPKLDAIKKRYADDPAKQQEATMKLYQEEKVNPMAGLS
                     GCLPMLLQLPVFICLVKVLEMSIELRHAPFIFWIKDLSAPDPLTIVNLFGLLPFDPAH
                     VPLIGGFLNGPLHIGPVAILYGASMWLSQQMSPTTGMDPNQRMMMAFMPLIMVFFFSQ
                     LAIGLMVYYFWNNILTILQQYSIMRRLKVENPVDNFFARLSKKKAA"
     gene            9590..>9911
                     /gene="yihA"
                     /locus_tag="VF402_09195"
     CDS             9590..>9911
                     /gene="yihA"
                     /locus_tag="VF402_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX7800512.1"
                     /translation="MTDDAYDDETLEAARVLFARPVAFVMGAAAVAQLPGDALPEVAF
                     AGRSNVGKSSLINALVGQKHLARASNEPGRTRELNFFILNAALHLVDLPGYGWAKASK
                     TTVKK"
BASE COUNT         1694 a         3200 c         3338 g         1679 t
ORIGIN      
        1 tcgctggacc tcgcctatgt cgccgccggt cagttcgacg gctactggga gcgcggcatc
       61 aagccgtggg acatggcggc gggccttttg atggtccacg aagcgggcgg caagttcgcc
      121 tcgatcgatt ccgacgccag cccgttcgaa accggcacgg tgatctgcgc caatctcgac
      181 ctgttcccgg aatttctcga aaaggtgcgg ctggcgaagt agtcagcggg tattgcccgg
      241 atcgttgaag aagatcaggc tgagcgccag cacggcggcg gcgtcgcgct ccggccccgg
      301 cgccttcggc agatagacgg tgggttgcag gccggtcttg cgcaccgccg ccacgggcgt
      361 tccgtccgga cgcgcgatca ggtagtccga ggtctggccg ttgctgagcg tcagcccgcc
      421 caaaagttcg gtggtgacgc gcaacgtgac ctcgccatag tgaaattcgc cgccgcgctg
      481 cggctgagcc agccggttta aaagcttgcc gccggtcgac agggccaggt ccagttcggc
      541 gtccttgggc gccgcgccgc catagctgca gatataggcc agttcgtcgc gcttgaaggc
      601 cagccagtcg aggccgatcc gcccctgtcc gcccgcgcag tccgccgtca cgccgtcgcc
      661 cagacccgga ccggtgacgg tgaagccggt tttgaacttg tccgaggtga acaggccgag
      721 gccctcggcg tggcgcgtcg cggcgtggat ttcgccggca taggcgccca gggcgtagcc
      781 gggatggaac agatcgtggc cttttttgcc ctgaagcttg atttcgtcgg tcgtggggcg
      841 caaccggtcg aggatcgact gtccggcgtg ggccgagctt gtcagggcga acgccgcgat
      901 cagggccaga cagacaggac gcatgagatt tcccccgttt tcgtcaccgg gggcaggctg
      961 gcataactgc gttacgcggt caatcttgcg cggcggcgcg cagggccttt ttcagcgcgg
     1021 tcacgcctgc cgcagcgccg tccgggcagt tccagaccgc gccggacacg gcgatgaagt
     1081 cggccccggc ctcggcgacg gcgcgggcgt tggccgcggt gatcccgccg atggcgacgc
     1141 agggcgtctg catcgtttcc tgccagattt ccagcgtctc cagctccgcc atatgctcga
     1201 ccgtcttggt cgtcgtcggg aagaaggcgc cgaaggcgac gtaatcggcg cccgcctcgg
     1261 cggcctccat cgccagatgg cggctgttgt ggcaggtcac gccgatcatg gcgtccggcc
     1321 cgagcaggcg gcgcgcctcc ttatagggcg tatccgactg gccgacatga acgccgtcga
     1381 ggcccagctg cttcgccagc atggcgtgat cgttcatcaa caccgctgcg ccctgcgcat
     1441 gggcgatcgg cgtcagcacg cgcgtcacgg cgcggatatg cgcctcgtcc acgtccttca
     1501 gccggatctg caaggcggcg acggtttcgc cgcccgccgt cagcgccgct tgcagcgcgc
     1561 gcgcgaaggc gtccgcgtcg tcgatgcgcg gcggggtgat caggtagagg cggcaatcgg
     1621 tcatgcgccc tgctttgcct gagacgcggg cgggcggcaa gccgaatttg cgctttcgtt
     1681 tgagaatgac ttgcaatacg atcaagcctg cgctatatga gaagcattct cattatcagg
     1741 gtgtgctttt cgtgtacgtc tgcaattgta acggcatccg cgagcgcgac gcgcttcagg
     1801 tcatcgagtc gggggcggcc acggtccgtc aggtgttcga cggttgcggc gcccgtccgc
     1861 agtgcgcaag gtgcgtctgc gacatccgcg acatgctgga tgaggccgga tgcgcgatgg
     1921 cgatcgcggc ggaataattc ctgttttcgc tttacccgaa ccgccgcccg gcactatgct
     1981 ggcggcggtt ttttattgga cgcggagcgc cgacatgcag ggtcaacccg aaatcatcaa
     2041 gctgctcaac aaggtgctga ccaacgagct gacggcggtg aaccaatact tcctgcacgc
     2101 gcgcatgatc gagaactggg gcctctatca cctcggccgc atcgtctata acgaatcgat
     2161 cgaggagatg aagcacgccg atcttttgat caagcgcatc ctgttcctgg acggcctgcc
     2221 caatcttcag gacctgcaca agctgcgcat cggcgaaacc atcggcgaat gcctcgcgtc
     2281 ggacctcagg gtcgagaccg gcgggcgcga cacgctggtc gcgggcgtca agctgtgcga
     2341 ggacagccag gactatgtca gccgcgacct gctggccagg attctgtccg acaccgagca
     2401 ccatatcgac ttcctggaaa cccagctcaa cctgatcagg ctgatgggcg agccgaacta
     2461 tatccagagc gcgatgaagg aaatcgaggg cgaggcgtaa aagcccgcat taaccacttt
     2521 gacctttgca gcggcagcgc ctagtttgcc tgtgaaaacg ggcaggggac gaacagattg
     2581 ccagacttca gccgccgggc actgatgacg ctgctgggcg cgtgcgcctt gacgggactg
     2641 gccttgacgg ggctggccgc gtgcggccgc ccggacgggg cattttcgga tgatcgggcg
     2701 ccggacgatt ccgacgcggg cgacgacgcc agcgagacga tcgatcccag gctggcgcaa
     2761 cagcagggca cgctgcaatg ggcggtgtcg ggcgcctggc gcaacgcggt cgacaaggcg
     2821 cgcgacaaat atcagcaccc gatcgagacg ctgcaattct tcgaggtcga tcccaaggcc
     2881 acggtggtcg atatgtggcc gggcgccggt tacatgaccg aggtgctggc gccgtacctg
     2941 aacgcgggca agggcgaata cgtcgcggcg ctgatcgcgc cccgtcccgg cgatcaggcg
     3001 atccaggcgc tgaacgacgc ctaccgcaag catttcgacg ccgacaaaaa gctgtacggc
     3061 gacataacct acagccagtt cggcgccgac accgggccgc tggtcccggc ggggacggcg
     3121 gactgcgtgc tgatgatcac ggtgattcag gactggatgg cgtcgggcgt cgtcgaaaaa
     3181 gccttcgccg acgccttcgc ggcgctgaag ccgggcggcg tcctcggcgt cgagcagcac
     3241 cgcgccgaca tcggcaacgt gcaggatccg gcggcgacca acggctacgt gcaggagccg
     3301 ttcgtgcggc aactggccgc cgaggcgggc ttcgccttcg tcgccgcgtc cgagatcaac
     3361 gccaatccga aggacgacaa ggaccatccg ttcggcgtct ggaccctgcc gccgcagcgc
     3421 ctgaccgcgc cgcgcggcca gccgcccaac ccggatttcg acggaaccct ttacgaatcg
     3481 attggagaaa gcgaccgtat gaccctgaag ttcaggaagc cgtcatgagt cgcatagccg
     3541 ccctttccac cagcgcgccg ctgatgttca cgccggagtc gcgcccgccc gaaggcggcg
     3601 tgggcgaatg gctgcgcggc gcgggcggcc tgcgcctgcg cgtcggctac tggcccgcgc
     3661 cggaaggcgg gacgtccgag ggggcgtcgc gcggcacggt cttcatctcg ccgggacgtt
     3721 cggagccgat cgagaaatat tacgagctgg cgcgcgacct gatcgggcgc ggcttcgatg
     3781 tggtggcgca cgactggcgc gggcaggggc tgtcggcgcg gctgctgccc gaccggctga
     3841 agtgccatgc gcgctcggcg gacgaattcc tcgacgattt tcagcgcctg ctcgacgcct
     3901 tcgaggagcg cgcgcccaag ccgtggatcg cgctgggcca ttcgatgggg gcggcgctga
     3961 acatggccac gctggtcaag ggcgagccgc gcatctccgg cgccctgctg gtcaatccga
     4021 tgctgcgcat caagaccggc aaacattcgc tgtggtcggt gaattttcag gtcaactggc
     4081 aggtcaatca tggacgcggc gccgactatg tgccggagct gttcgaggat ccgttcgaaa
     4141 acaccttcga gaccgacgcc ctgacccacg acccggcgcg gtacgagcgc tggcgcgaac
     4201 agttgttcgc ctgtccgcac ctggcggtcg gctcgccgac ctgggggtgg ctgcaattcg
     4261 cgctgcgcct cggcgagacg ctgctgcgcg acaagtataa gctgctcaag aaggtcagga
     4321 cgccgatcac cttcgtctgc tccggcgacg atcacgtcat cgcgcacccg ccgacgcggg
     4381 ccttcgcgcg caagatcgcc ggggcgcagt atatcgaggt cgcgggcgcc gagcatgaac
     4441 tgctgatcga ggcggacgaa tatcgcgatc aggcgctcag ggcgttcgac gagttggccg
     4501 atttcgtcgc gccgcgcggc ggggggacgg aggccgggga tgcgccggtc ggggaggacg
     4561 ccgaggcgga ggagcaggcg gggtagggat aggatacccc tccaccacgc cctgcgggcg
     4621 cggtccccct ccccatcctt gatggggagg agagaaggcg gcttgcgatt acgagatcgc
     4681 cacggctttg cagccttcgg cttccagcag ttcgaccagt tcgatgaagg cgtcgagcgg
     4741 ggcggtgccg aaatcgacgt tcatgccgtc cgcgtccagg gtcagggcgt ggatgttgcc
     4801 ggaaaagcgc gtgtcggggt cgaagcgttc ctcgttcggg tcgaaccagg tttcgaacca
     4861 cggcgcgatg cggtcgggcg ccacgtgcgc gccctgcggc agcgagaagg tcatgtcgtg
     4921 ccaggccagc cgcgtcaccg ccatctccat ctcgccgaag ctgacctgca acggatcgaa
     4981 gctgatctcc tcgtccggcg acagttcgac gacgtggccg ccgtcctcgg gcgaggcgta
     5041 gtcggcgcaa taggcgtggc cgaagaagtc gctgtcctcg gtcagctcga atttcacctc
     5101 ggggccgccg ttgtcgtgac cggccctctg ctggtcgacg aaggcggaaa actgggtgat
     5161 gtagctgtcg cgcacgaggt tgagcaggtc gccgaattcc aacaaaagca ctttcctaaa
     5221 agtgtgacgc acttttagga tccaaaagtg cgttaaagcc aatatgggag cgccaaatcg
     5281 atgtcatcga tctggcgctc caattatcct ttcagcggca ggcggatttc gaaggtggtg
     5341 ccgtcggggc cggtctggac cagacgcacg tcgcccccgt gcaactgcgc cagttcgcgc
     5401 gagatggcga ggccgaggcc cgacccgccc ggcgtcgccg acgaggagaa cggctggaac
     5461 agcttgtcgc gcgcgcgttc ggggatgccg gggccgttgt cggcgacgat cagcaccacg
     5521 tcctgcgcgg tcttgatggc ggtcagggtc acgcgcccgc gcgtcttgcg gttgggttgc
     5581 agttcgatgg cctgacgggc gttgcgcatc aggttgacca gcaggcggtg cagttgctcc
     5641 gggtcggcct cgaggcagaa gccgcgcggc aggcgggcga tgaagcgcac gttctccgcc
     5701 gcctgcggcg tgacgccgag gcccgcgtcc tcggcggcgg cgtcggccag ttcgcgcagg
     5761 cgcacgatct ggatttccgg cgcgcgctcg tccgacttgc cgtaggtcag cacgttctgc
     5821 gccagcgaca gcgcgcggtc cagcgcgcgc tccagccgcg gcagcgcctt ggcgacggcg
     5881 ggatcggccg aggccgccag ccggtccgac gccatctgcg ccgaggtcag catgttgcgc
     5941 aggtcgtggt tgatcttgga caccgcctgc cccagcgcgg cgaggcgggc gcgcgaacgc
     6001 agcgcctgac gcacctgctc ctgcatcgag gccagttcgc gctcgatatg gccgatctcg
     6061 tcgcggcggc cggacagttt cggcgtcgcg gacgggtctt cgggattggt gcggaaggcg
     6121 gcgatgccgt cggtcagctt acggatcggg cgcacgatga aggccgacag ggcggcgaac
     6181 accagaatcc cggcggccag cgagatgctc agcgacatgc gcaacatgcc gatcaggctg
     6241 gattgcagcc aggtcttgag cggcgcggcg cgcaccacga tctcgatcat gtcgccggag
     6301 cgcacgcgcg gcttggcgat caggtggatc agccggtcgg gcttgcccat cagcgtctcc
     6361 cacggcgccc acatataggc gaggtcgtgg cgcagatcct tgtgcgaatc gcgcatgtcc
     6421 agcacctcgt ccgggatttg caggtcgggc aggggattga gcatgtaata gcggtcgccg
     6481 ccctgcaggc gcagatagag gacgcccgcg ctgcgcagga gctggaacgc ggtggcgcgc
     6541 gagacgttgt tggcggtggc ggtgtcgacc gcctgcgagg cgatctcggc cgagcgcacg
     6601 cggtcggtca gccagtcctc ctgatcggcg gccagacgcg gaatcacgat catcacctcg
     6661 acgatcacga cgaagacgag ggtgaggatc agcaactggc cggacaggga gaaggcgaag
     6721 tcgcgcgcgg ccttgaaggc gcgggttccc gcctcgaaac cgagtcgccc cagcgccgcc
     6781 gcggcgtgag ccaggcggcg cgcgtaaaag ccctgccggg gcgagggacg tccccccggc
     6841 cccgagggga gatttaaatc tgtcttgccg ggatcgaaca cgatttgtta ttttcgacgg
     6901 gcggatggag gtttgcgcca gtatagcgcg tttttccgtg atttggcggg aatttacgca
     6961 aaaagacgat acgaaaagcg cgcccggtcc gccctttgcc ccccattagg cgttgacaag
     7021 cgaaagccgc tggactatcc acgcgcctcc ttacgcaatt tccggccgcc agcgcgttgc
     7081 gcgcatccgg cgggccgcca ccttaccgga gcctcctgtc atgggaacga aacgcaccta
     7141 tcagccgagc cgcctcgtgc gcaagcgccg ccacggctat cgcgcccgca tggccaccaa
     7201 gaacggccag aaggtcgtgg cgcgccgccg cgccaagggc cgcaagcgcc tgacggctta
     7261 acgagcgcct tttcacgctt gtgatgacag gaagagtccc tatgttgcgt aggggctctt
     7321 tttctgtttc cggccggctg tctcccgcat gatttcccgc atcaaggtcc gcagcgattt
     7381 cgtcgccgcc tccaaggcgc cgtggcaggc ccgacccggc gtggtggtgc aggcgcgtcc
     7441 gcgctgcgac gatgcggcga tgcgcgcggg cttcaccgcc accaaaaagg tcggcaacgc
     7501 cgtgatccgc aaccgcgcca agcgccggtt gcgcgccgcc gccgaggcca tgctgccgct
     7561 ctacggccag cccggccacg actatgtctt cgtcgcgcgc gcgcagacgc cggcgcgcga
     7621 ctggcaggct ttacttgacg atgtcaaagc cgccctgtta aggctctccc gcccgcaaag
     7681 ccaacgcgac aaccggcccg gacccgtcgc cgcgccctcc catgcccctc aggacaaatc
     7741 cgctccatga acaaagacga caacaacagg aatatgatct tcttcctgat cgccagcatg
     7801 gttctgcttg cggcctatca gtatttcgtc ctgtcgccgc agcagaagaa gcagcaggcc
     7861 gcgcaggccg ccgccgccgc gtcttcgtcc agcgccgccg ccgcgggcct gcccatgccg
     7921 ggccagccgg tcgtgctgac gcgcccgcag gcgctggcgc aatcgccgcg cgtgcccatc
     7981 gacacgccgt cgctgaaagg ctcgatcgcg ctcaaggggg cgctgctcga cgacctctat
     8041 ctggcgaagt accatgaaac ggtcgatccg aagtcgccgc tggtcgagct gctgagtccg
     8101 gcgggcacgg cgcatcccta ctatgcggag gccggttatt tcggccagaa cgtgccgaac
     8161 ctgccgggca tcggcgcgca gtggacgctg gtttccggcg acaaactggc gccgggcaag
     8221 ccggtgaccc tggcttacga caacggcgcg gggctgaaat tccagcgcac ctacgccatc
     8281 gacgacaact atatgatcac ggtgaccgac agcgtggcca acaccggcgc gcaggccgtg
     8341 cagctcgaac cttacgccag cgtgacgcgc gtgggcatgc ccgtcgaggt cgggcgcagc
     8401 ggctacgtga tggagggcgc gatggcgacc gtgtccaagg acgcggcgga cgccaaggcc
     8461 ggttatcaca gccagtccaa gcgctataaa gacctgatca aggacaagac gccgccgcag
     8521 ggccagtcga tgggcggctg gtacgccgtc accgacaagt actggatggc ctcggtgatc
     8581 cccaatcagg ggcaactggt caactattcg ctgtcggcga ccgccttgcc cgacaagacg
     8641 ccggtgttcc gcgcgggcta ttccgacgcc gcggtcaccg tggcgccggg cgcgacctgg
     8701 acgtcgcaga gccatatctt cgccggcgcc aagctgcacg accagttgaa cgcctatcag
     8761 aagtcgctga acctgccgcg cttcggctac gcgctcgact gggggcatct cgactggctg
     8821 accattccgt tctacctgct gctggtgcag ctttataagt ggatcggcaa tttcggtttc
     8881 gccatcctgg cgctgaccgt ggtgatcaag atcatcttct atccgctggc gcaccgctcc
     8941 tatgtggcga tgatgaagat gaagcacgtc cagcagcatc tggcccccaa gctggacgcg
     9001 atcaagaagc gctacgccga cgatcccgcc aagcagcagg aagcgacgat gaagctgtat
     9061 caggaggaga aggtcaatcc gatggccggg ctgtcgggct gtctgccgat gctgttgcag
     9121 ttgccggtgt tcatctgcct ggtcaaggtg ctggaaatgt cgatcgaact gcgccacgcg
     9181 ccgttcatct tctggatcaa ggatctgtcc gcgcccgatc cgctgaccat cgtcaacctg
     9241 ttcggcctgt tgcccttcga tccggcgcat gtgccgctga tcggcggttt cctcaacggg
     9301 ccgctgcata tcgggccggt ggcgatcctg tacggcgcct cgatgtggct gagccagcag
     9361 atgtcgccga ccaccggcat ggacccgaac cagcgcatga tgatggcctt catgccgctg
     9421 atcatggtgt tcttcttctc gcaactggcc atcgggctga tggtctatta tttctggaac
     9481 aacatcctga ccatccttca gcaatacagc atcatgcgcc gcctgaaggt cgagaacccg
     9541 gtcgataatt tcttcgccag gctgagcaag aagaaggccg cctagaggca tgaccgacga
     9601 cgcttacgac gacgaaacgc tggaggccgc gcgggtgctg ttcgcccgtc cggtcgcctt
     9661 cgtcatgggc gccgccgccg tggcgcaact gcccggcgac gctttgccgg aggtcgcctt
     9721 cgcgggccgc tccaatgtcg gcaagtcgtc gctgatcaac gccctggtgg gacagaagca
     9781 tctggcgcgc gcctccaacg agccggggcg gacgcgcgag ctgaacttct ttatcctcaa
     9841 tgcggcgctg catctggtcg acctgcccgg ctacggctgg gccaaggcgt ccaagacgac
     9901 ggtgaagaag t
//
LOCUS       DASVLL010000555         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_412302,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000555 DASVLL010000000
VERSION     DASVLL010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412302"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VF402_09200"
     CDS             complement(<1..1454)
                     /locus_tag="VF402_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800513.1"
                     /translation="MSAAAARYIAVLAAVLLATAGLAAAFGYVSVDSWYYMLLAQSLR
                     VGHPLQLHGQYMAVYPLGYPALLALTAPLARPEVMMITAKLVNIALLGADVWLIWRAS
                     GQLAMATLIAVNPVTLGVAMYSWSENLELFAICAAICLIARLAEGADRTRLALLAGAL
                     IAGVFSRYFFSPFAFLLFVATWLVYGRRVAWRVFPAFCAAGAVYLAYQGVNWAVTGYA
                     TGMPRHAAPEAPLLLIRQFLTAFGWSGLKLAIAAALLCGLARCARRRGDEDRRAATLV
                     LMAGLAFLALAFALRLRTYFDPFNTRTLGYGVVLLAAGLAGRYAAPRKAWPVAALVAC
                     GLFSVLFADDFAIPQDLHDLVTDPGDYSFPAASLAALRGPAPPADVIVSFQLPVTGLD
                     DADVDNVPEVWYGPDVQTLAPLGAPDNPPEHVPAFLKRLAALHPKACAVDFTPFATAA
                     DFDAWLDARYLVDRHWSFHDFRYHKVKIDTLAPD"
     gene            1643..>2586
                     /locus_tag="VF402_09205"
     CDS             1643..>2586
                     /locus_tag="VF402_09205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7800514.1"
                     /translation="MLQAYLAKLIKRGRLTVRVNPNVTWTFGEAIADRPDLDIAIWFS
                     SKWAAAKVGLHPYLYFGEAYMDGDLIVERGDLWSFMELIGINMQSLPPRSGFMRFLNR
                     LRQYLPAHSLAASRRNVAHHYDLSERLYRLFLDEDMQYSCAYFRRDDMTLEEAQTAKR
                     DHIADKLRLSPGQSVLDIGCGWGGMALHLAKRANVNVTGVTLSTEQLAVAEQRAQGPD
                     VNSRVRFALCDYRQVEGQFDRIVSVGMFEHVGKAAFDTYFDTVYDRLKDDGVALIHSI
                     GRRGPSGARSSWLDKYIFPGGYIPSLSEIMTSVEKSGL"
BASE COUNT          488 a          854 c          870 g          374 t
ORIGIN      
        1 atgtcgggcg ccagcgtgtc gatcttcacc ttatgatagc ggaaatcgtg aaacgaccag
       61 tgccggtcga ccaggtagcg cgcgtccagc caggcgtcga aatccgccgc cgtggcgaac
      121 ggggtgaaat cgaccgcgca ggctttcgga tgcagcgccg ccagccgctt caggaaggcg
      181 gggacgtgtt ccggcggatt gtcgggcgcg cccagcggcg caagggtctg gacgtccggg
      241 ccgtaccaga cctccgggac gttgtcgaca tcggcgtcgt cgaggccggt caccggcaac
      301 tggaacgaca cgatcacgtc ggcgggcggc gcggggccgc gtaaggcggc cagcgatgcc
      361 gccgggaagc tgtaatcgcc ggggtcggtc accaggtcgt gcagatcctg cgggatggcg
      421 aaatcgtcgg cgaacagcac cgagaacagg ccgcaggcga ccagcgccgc caccggccac
      481 gccttgcgcg gcgccgcgta acgcccggcc agcccggcgg ccagcagcac gacgccatag
      541 ccgagggtgc gggtgttgaa cggatcgaaa taggtgcgca ggcgcaaggc gaaggccagc
      601 gccagaaagg ccagcccggc catgagaacg agcgtcgccg cgcgccggtc ctcgtccccg
      661 cgccgccggg cgcagcgcgc gaggccgcac aaaagggcgg cggcgatggc gagcttcagc
      721 ccgctccagc cgaaggcggt caggaactgg cggatcaaca gcagcggcgc ttcgggcgcg
      781 gcgtggcgcg gcatgccggt cgcgtagccg gtcaccgccc agttgacgcc ctgataggcc
      841 agatagacgg cgcccgccgc gcagaaggcc ggaaagacgc gccacgcgac ccgccgccca
      901 tagaccaacc acgtcgcgac gaacagcagg aaggcgaagg gcgaaaagaa gtagcgcgaa
      961 aacacgcccg cgatcagcgc cccggccagc agggccagcc gcgtccggtc cgccccttcg
     1021 gccagccgcg cgatcagaca gatcgccgcg cagatcgcga acagttcgag attttccgac
     1081 cacgaataca tggcgacgcc cagcgtgacc ggattgaccg cgatcagggt cgccatcgcc
     1141 agctgccccg acgcgcgcca gatcagccag acgtccgcgc ccagaagcgc gatattgacc
     1201 agcttggcgg tgatcatcat cacctccggg cgggccagcg gcgcggtcag ggccagcagg
     1261 gccggatagc cgagcggata gacggccatg tactgaccgt gcaattgcag cggatggccg
     1321 acgcgcaggg attgcgccag cagcatgtag taccaggaat cgacgctgac atagccgaag
     1381 gcggccgcca gccccgccgt cgccagaagg acggcggcca gaacggcgat ataacgggcg
     1441 gcggcggcgg acatggccgt ctaaggcgca gacccgaagc cgcctgtcaa tgcgtgccgc
     1501 atttaccgca tccttacgat gcgcgccgca atcgcgatgg gttttttgcg ctccgggctt
     1561 ttctttgcta gggtcagcgg caacatgcgc ttttccgggg cgcgcatgac gtcaggtttc
     1621 acgctttttg aagggaaaca ggatgttaca agcgtatctc gccaaattga tcaagcgcgg
     1681 ccgtctgacg gtccgcgtca atccgaacgt cacctggacc ttcggcgaag cgattgccga
     1741 ccggcccgat ctcgacatcg ccatctggtt cagcagcaaa tgggcggcgg ccaaggtcgg
     1801 cctgcatccg tacctctatt tcggcgaagc ctatatggac ggcgacctga tcgtcgagcg
     1861 cggcgacctg tggagcttca tggagctgat cgggatcaac atgcagtccc tgccgccgcg
     1921 gtcgggcttc atgcgcttcc tgaaccggct gcggcaatac ctgcccgccc actccctcgc
     1981 ggcgtcgcgg cgcaatgtcg cgcaccatta cgatctgagc gagcggctct atcgcctgtt
     2041 cctcgacgag gacatgcaat attcctgcgc ctatttccgc cgcgacgaca tgacgctgga
     2101 ggaggcgcaa accgccaagc gcgaccatat cgccgacaag ctgagattgt cgccgggcca
     2161 gagcgtgctg gacatcggct gcggctgggg cggcatggcg ctgcatctgg ccaagcgcgc
     2221 caatgtcaac gtcaccggcg tgaccctgtc gaccgagcaa ctggccgtcg ccgaacagcg
     2281 cgcgcaggga ccggacgtca attcgcgcgt gcgcttcgcc ctgtgcgact accgccaggt
     2341 cgagggccag ttcgaccgca tcgtcagcgt cggcatgttc gagcatgtcg gcaaggcggc
     2401 gttcgacacc tatttcgaca cggtttacga ccggctgaaa gacgacggcg tggcgctgat
     2461 ccattcgatc gggcggcgcg ggccgtcggg cgcgcgatca agctggctgg acaagtacat
     2521 cttccccggc ggctatatcc ccagcctgtc cgagatcatg acctcggtgg aaaaatccgg
     2581 cctgtg
//
LOCUS       DASVLL010000556         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_359328,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000556 DASVLL010000000
VERSION     DASVLL010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359328"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VF402_09210"
     CDS             complement(<1..1255)
                     /locus_tag="VF402_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7800515.1"
                     /translation="MSAAAKPADTPGGVLAAMQALAKPDKAFIDGHWAASHSGRTFDN
                     YSPRDGALINRIAACDAADVDAAVASARRAFADRRWRGLAPKQRKTILHRFADLMGEH
                     AETLALLECLDTGKPINNTRGFDIGAAINTVRYYAEALDKIYGEVAPTPDDRLSYVVH
                     EPLGVIGAVTPWNFPLHMAMWKVAPALAMGNSVVLKPAENASMSALYAAQLALEAGLP
                     PGVWNVTPGLGTEAGEALALHMDVDMIAFTGSGPVGRQLLRASADSNLKRVSLELGGK
                     SPQIVFADCDDLDQAAQNAAWGVFYNQGQVCTAASRLLVEASIKDAFVEKVAAIARTI
                     RVGDPFDPATRFGAMISERQMNTALDYIEKGLAEGGRLLAGGRRARPESGGYYVEPTL
                     LDDIAPDNVLAQEEVFGPVLSVLSFD"
     gene            complement(1373..2104)
                     /locus_tag="VF402_09215"
     CDS             complement(1373..2104)
                     /locus_tag="VF402_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7800516.1"
                     /translation="MAVVGLDTANTTGDDARQDARMHDQVYDRIAEALIQGRIPPGKS
                     VSLRGLAQSLGVSPMPVREAVRRLIAERALELQPANKRLRVPGLNAHRLTQLATARQW
                     VEPELAALAAGHATKEVIRRLRDDDAAVMAALTKGDVSAYMSSNQDFHFSIYRLAGAE
                     LFYDMARTLWLQVGPFMRVVFGRMGTVHLPKDHHQDMIAALERGDADAVRASMAADIA
                     EGMDLMMAAIAAHEDEAPVKRRGRR"
BASE COUNT          323 a          730 c          714 g          344 t
ORIGIN      
        1 tatcgaagct cagcacggac agcaccgggc cgaacacctc ctcctgcgcc agcacattgt
       61 ccggcgcgat gtcgtcgagc agggtcggct cgacatagta gccgccgctt tcgggacgcg
      121 cgcgccgtcc gcccgcaagc aggcgcccgc cttcggccag ccccttttcg atatagtcga
      181 gcgcggtgtt catctggcgc tcggaaatca tggcgccgaa ccgcgtcgcc gggtcgaacg
      241 ggtcgccaac cctgatcgtg cgggcgatgg cggcgacctt ttcgacgaag gcgtccttga
      301 tcgaggcttc gacgagcagg cgcgaggccg ccgtgcagac ctggccctga ttgtagaaca
      361 cgccccaggc cgcgttctgc gccgcctgat cgaggtcgtc gcagtcggcg aacacgatct
      421 gcggcgactt gccgcccagc tccagcgaca cgcgcttgag gttggaatcg gccgaggcgc
      481 gcagcaattg ccgcccgacc ggaccggagc cggtgaaggc gatcatgtcg acgtccatgt
      541 gcagggccag cgcttcgccc gcctcggtcc ccagccccgg cgtcacgttc cacacgcccg
      601 gcggcagccc ggcctccagc gccagttgcg cggcgtagag cgccgacatc gaggcgtttt
      661 ccgccggttt cagcacgacg gaattgccca tggccagcgc gggcgcgacc ttccacatcg
      721 ccatgtgcag cgggaagttc cacggcgtga cggcgccgat cacgcccagc ggctcatgca
      781 cgacgtaaga taagcggtcg tcgggcgtcg gcgcgacctc gccgtagatc ttgtcgagcg
      841 cctcggcgta atagcgcacg gtgttgatcg ccgcgccgat gtcgaagccg cgcgtattgt
      901 tgatgggttt gccggtgtcg agacattcca gcagggccag ggtctcggcg tgttcgccca
      961 tcaggtcggc gaagcggtgc agtattgtct tacgctgctt cggcgccagg ccgcgccagc
     1021 gccggtccgc aaacgcccgg cgcgccgagg cgaccgccgc gtcgacgtcc gccgcgtcgc
     1081 aggcggcgat gcggttgatc agggcgccgt cgcgcgggct gtaattgtcg aaggtgcggc
     1141 cggaatggga ggcggcccaa tggccgtcga tgaaggcctt gtccggcttc gccagcgcct
     1201 gcatggccgc cagcacgccg cccggcgtat cggcaggttt cgcggctgcg gacataaggg
     1261 cctcctgaca tggcgattgt gatcacaact agacaccggc cgccggtaaa aagtcaagcg
     1321 tgagaagcga cggcttcata tctcccatct ttaatgggga ggtatgagag gcttacctcc
     1381 gcccgcgccg tttgaccggg gcttcgtcct cgtgcgcggc gatggcggcc atcatcaggt
     1441 ccatgccttc ggcgatgtcg gcggccatcg aggcgcgcac ggcgtcggcg tcgccgcgct
     1501 ccagcgccgc gatcatgtcc tgatggtggt ccttgggcag gtgcaccgtg cccatgcggc
     1561 cgaacaccac gcgcatgaac ggcccgacct gtagccacag ggtgcgcgcc atgtcgtaga
     1621 acagctccgc cccggccagc cggtagatcg agaagtggaa atcctgattg gacgacatgt
     1681 aggccgagac gtcgcccttg gtcagcgcgg ccatcaccgc cgcgtcgtcg tcgcgcaggc
     1741 ggcggatgac ttccttcgtc gcatggcccg ccgccagcgc ggccagttcc ggctcgaccc
     1801 actgccgcgc cgtggccagt tgcgtcaggc ggtgcgcgtt cagccccggc acgcgcagcc
     1861 gcttgttggc cggttgcagc tccagcgcgc gctcggcgat caggcggcgc accgcctcgc
     1921 gcaccggcat gggcgagacg cccagcgatt gcgccagacc gcgcaaggag acggatttgc
     1981 cgggcggaat ccggccctga atcagcgcct ccgcgatccg gtcatagacc tgatcgtgca
     2041 tccgggcgtc ctggcgggcg tcgtcgcccg tcgtgttcgc cgtatccagc ccgacgaccg
     2101 ccatgcctga a
//
LOCUS       DASVLL010000557         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_252200,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000557 DASVLL010000000
VERSION     DASVLL010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252200"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VF402_09220"
     CDS             complement(<1..340)
                     /locus_tag="VF402_09220"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX7800517.1"
                     /translation="MPAEPLSFQDLILTLHAYWSEQGCAILQPYDVEVGAGTLHPATV
                     LRALGKKPWRAAYVQPSRRPVDGRYGENPNRLQHYYQYQVILKPNPENLQELYLGSLK
                     AIGLDTHLHDI"
     gene            474..1769
                     /locus_tag="VF402_09225"
     CDS             474..1769
                     /locus_tag="VF402_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800518.1"
                     /translation="MQLSPDLSALRATLKRPTSWLLIALGFSSGLPFLLVGSTLNLWL
                     RGSGVSLSVIGFVSWASLLYGLKILWAPWMDKAHIPVLHRWLGQRRSYMLLSQIGVAI
                     GLCAMALIGPSHLPAFVAAAVFVTFCAASQEIAVDAWRVEETKSESDQAFNPSAYSLG
                     VKIGLWSASSVILIAGNHFGWPLTYEIMAGALIVGVIATLAAHRTEAEQRYDAEPKSF
                     RQLILTPFQSFFAEHAGTAGLILVTLAFYRLGDYLIGPVCTPMYQDTGLDLDAIGALR
                     GTVGLAAGFIGIGIGGACLYFLGLQRAFWLGALVGPASNLFFSWMSLVHGSLTLFGAA
                     LVVDDIGDGIAETALVAFMTKMTGRGHTLTHYALMYSVAALTGKFLKGFSGLLIDSLK
                     PSIGLFPAYAAFFAFTAALALPTLFLCWRLRLSGVFTKR"
BASE COUNT          294 a          608 c          566 g          376 t
ORIGIN      
        1 gaatgtcatg caggtgcgta tccagcccga tcgccttcaa cgagccgaga tacaattcct
       61 gcagattctc cgggttcggc ttcaggatca cctgatactg gtaatagtgc tgcaaccggt
      121 tcgggttctc gccatagcgc ccgtcgaccg ggcggcgcga cggctgcaca taggccgcgc
      181 gccacggctt tttccccagc gcgcgcagca cggtcgccgg atgcagcgtg cccgccccga
      241 cctcgacatc gtagggctga agaatcgcgc agccctgttc ggaccagtag gcgtgaaggg
      301 tcaggatcag atcctgaaag ctcagaggtt cggcgggcat gaaaacggcg tatccggtgg
      361 tcgaaaattg gcgcgaacat actggcccgc cccctccgcc gcaagccata agaccaaaca
      421 atagcttcgc tttctgcgca gaatcggcct tgatccttgc aggtgagaag ataatgcagc
      481 tttcccccga cctgtccgcc ctgcgcgcga ccttgaaacg gccgacctcg tggcttctga
      541 tcgcgctcgg cttctcgtcc ggcctgccgt tcctgctggt cggatcgacc ctgaacctgt
      601 ggctgcgcgg ctctggcgtg tcgctgagcg tgatcggttt cgtctcgtgg gccagcctgc
      661 tctacggcct gaaaatcctg tgggcgccgt ggatggacaa ggcgcatatc cccgtcctgc
      721 accgctggct gggccagcgc cgctcctaca tgctgctgag tcagatcggc gtcgccatag
      781 gcttatgcgc gatggcgctg atcgggcctt cgcatctgcc cgccttcgtc gcggcggcgg
      841 tgttcgtcac cttctgcgcc gcctcgcagg aaatcgctgt ggacgcctgg cgggtcgagg
      901 agacgaaaag cgaaagcgat caggcgttca accccagcgc ctacagcctc ggcgtcaaga
      961 tcggcctgtg gtcggcctcc tccgtcatcc tgatcgcggg caaccatttc ggctggccgc
     1021 tgacctatga gatcatggcc ggcgcgctga tcgtcggggt gatcgccacc ttggccgcgc
     1081 accgcaccga ggcggagcag cgctatgacg ccgagccgaa atcgtttcgc caactgatcc
     1141 ttacgccgtt ccagagcttc ttcgccgaac acgccggaac cgccgggctg atcctcgtca
     1201 ccctcgcctt ctatcggctg ggcgactatc tgatcgggcc ggtctgcacg cccatgtatc
     1261 aggacaccgg cctcgatctc gacgccatcg gcgcgctgcg cggcacagtc gggctggcgg
     1321 cgggctttat cggcatcggg atcggcgggg catgtctgta tttcctcggc ctgcaacgcg
     1381 ccttctggct cggcgcgctg gtcggaccgg cctccaacct gttcttcagc tggatgtcgc
     1441 tggtccacgg ctcgctcacc ctgttcggcg cggcgctggt ggtcgacgac atcggcgacg
     1501 gcatcgccga gacggcgctg gtcgccttca tgacgaagat gaccggacgc ggccacacct
     1561 tgacccacta tgcgctgatg tattcggtgg cggcgctgac gggcaagttt ctcaagggtt
     1621 tttccggcct gctgatcgac agcctgaaac cgtcgatcgg cctgtttccg gcctatgccg
     1681 ccttcttcgc cttcacggcg gctctggccc tgccgacgct gtttctgtgc tggcgattgc
     1741 ggctgagcgg cgtctttaca aagcgctgag cgcgcgtaag gtctgatcct ctttaaggta
     1801 ggaaaccatg aaaatcgcca acacctttat caccgtggcg gacg
//
LOCUS       DASVLL010000558         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_198732,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000558 DASVLL010000000
VERSION     DASVLL010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198732"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1912
                     /gene="purH"
                     /locus_tag="VF402_09230"
     CDS             299..1912
                     /gene="purH"
                     /locus_tag="VF402_09230"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX7800519.1"
                     /translation="MPAAPDFPPAADLVTPRRALISLSDKTGLVEAAQALVAAGVELI
                     STGGTRAAIHKAGLPVKDVADMTGFPEMMDGRVKTLHPMVHGALLAYRDAPEHAKALS
                     EHNIAPIDIVWIDLYPFEQTVASGGGFEAAIENIDIGGPAMIRSSAKNHPYVAICVDK
                     AGMDEVLAAVKDKGATGMALRKSLAARAFARTAAYDSAVSTWFADQLGDTYPQRKTIA
                     GERVQTMRYGENPHQSAAFYRTNEARPGVTTAKQLQGKELSYNNVADTDAAIELVAEF
                     DPAQSAACVIVKHANPCGVATGPDLITAYKRALECDPVSAFGGIVALNRRLDKATAEK
                     IVEIFTEVVVAPEVDDDAAAVFAAKKNLRLLVTSALPDPLAGGQVFRSVAGGMLIQSR
                     DAARVTAADLKVVTKRQPTPTEIEDMLFAFTVAKHVKSNAIVYAKDGRSVGIGAGQMN
                     RRDSSRIARLRAQEAAETLGLSDSLAKGSACASEAFFPFPDGLLEAVAAGATAVIQPG
                     GSIGDQAVIDAADEAGIAMVFTGTRVFRH"
BASE COUNT          362 a          723 c          659 g          318 t
ORIGIN      
        1 ggtcggcaac agcgtcgcta aggataaggc ccgcgaggat tgctaccaga cgcgcatgca
       61 gccggtgcgc cactccggct acgaccggca caattacgac cgctatgacc gctacgaccg
      121 caacgatggc cgctatgggg ctcgccggta aacgaccggc ttgagccggc ggccatcgca
      181 ggaggccctg ccgaccagag gcggcagggc cttttctcat gcgctcaggt tggcaaaccc
      241 gacccggtcg tgtaaaccgc gcggcgttcc ctccctctcg cagtcaacgg attccgccat
      301 gcccgccgcg cccgattttc ctcccgccgc cgacctcgtc acgcccaggc gcgcgctgat
      361 ctcgctgtcc gacaagaccg gtcttgtcga ggcggcgcag gcgctggtcg ccgcgggcgt
      421 cgagctgatc tcgaccggcg gcacgcgcgc ggcgattcac aaggcgggcc tgcccgtcaa
      481 ggacgtggcg gacatgaccg gctttccgga aatgatggac gggcgcgtca agacgctgca
      541 cccgatggtg cacggcgcac tgttggccta tcgcgatgcg ccggaacacg ccaaagcgct
      601 gagcgaacat aatatcgcgc ccatcgacat cgtgtggatc gacctctatc cgttcgagca
      661 gaccgtggcg tccggcggcg gcttcgaggc cgccatcgag aatatcgaca tcggcgggcc
      721 ggccatgatc cgctcctcgg ccaagaacca cccttacgtc gccatctgcg tcgacaaggc
      781 gggcatggac gaggttctgg ccgccgtcaa ggacaagggc gcgaccggca tggccctgcg
      841 caagtcgctg gcggcgcggg cgttcgcgcg cacggcggcc tacgattccg ccgtctcgac
      901 ctggttcgcc gatcagttgg gcgatacgta tccgcagcgc aagaccatcg cgggcgagcg
      961 cgtccagacc atgcgctacg gcgagaaccc gcaccagagc gcggccttct accgcacgaa
     1021 tgaggcgcgc cccggcgtca ccaccgcgaa gcagcttcag ggcaaggaac tgagctacaa
     1081 caacgtcgcc gacaccgacg cggccatcga gctggtcgcc gaattcgacc cggcgcaaag
     1141 cgcggcctgc gtcatcgtca agcacgccaa tccgtgcggc gtcgccaccg gcccggacct
     1201 gatcaccgcc tataagcgcg cgctggaatg cgatccggtg tccgccttcg gcggcatcgt
     1261 cgcgctcaac cgccgcctcg acaaggcgac cgccgagaag atcgtcgaga tcttcaccga
     1321 ggtcgtggtc gcgccggagg tcgacgacga cgcggccgcc gtcttcgccg ccaagaagaa
     1381 cctgcgcctg ctggtcacca gcgccctgcc cgacccgctg gccggcggtc aggtgttccg
     1441 ctcggtggcg ggcgggatgc tgatccagag ccgcgatgcg gcgcgcgtca cggcggcgga
     1501 cctgaaggtc gtcaccaaac gccagccgac gccgacggaa atcgaggaca tgctgttcgc
     1561 cttcaccgtc gccaagcacg tcaagtcgaa cgccatcgtt tacgccaagg acggccgttc
     1621 ggtcggcatc ggcgcgggcc agatgaaccg ccgcgactcc tcgcgcatcg cccgtctgcg
     1681 cgcgcaggaa gccgccgaga ctctgggcct gagcgacagt ctggccaagg gctcggcctg
     1741 cgcgtccgaa gccttcttcc ccttccccga cggccttctg gaagcggtcg cggcgggcgc
     1801 cacggcggtg atccagccgg gcggctcgat cggcgatcag gcggtgatcg acgccgccga
     1861 cgaagccgga atcgcgatgg tcttcaccgg cacgcgcgtc ttccgccact aaaggatacg
     1921 gcgcgcggcg ctttagagcg cttttcgatc tgatggaatc agatcgaaaa gcgctctaaa
     1981 tatttgtttt gtcgctcttc cttatccaaa aagtggagcc actttttggg gaaacgcttt
     2041 aaccaccgcg cgccgccctt ac
//
LOCUS       DASVLL010000559         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_91224, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000559 DASVLL010000000
VERSION     DASVLL010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91224"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..951
                     /locus_tag="VF402_09235"
     CDS             70..951
                     /locus_tag="VF402_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7800520.1"
                     /translation="MTPAIVTEKLYARQGRFALGPLDFSAPRGAITALIGPNGAGKTT
                     LLDLLMGLGRAQSGRIELLGLTQPRDDAAIKARVAYVNPDLNYAVWGTVGRALDFVRG
                     FYPDWDEARCARLLTAFELARGQKIAALSFGARIKLALTMALAREAELLLLDEPTIGL
                     DVNARRVLFHELLEVVKAENRAVVISSHQLSDLERLADHCAILANGRLAAMAPMDGLL
                     ARYVQVDAVCAGRLPETPGARLIARNGDRVRLLLERARCDEAALAAQGVRVVAEVPLN
                     LEDLFVALTAEPLEAAA"
     gene            948..>1564
                     /locus_tag="VF402_09240"
     CDS             948..>1564
                     /locus_tag="VF402_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800521.1"
                     /translation="MMSPRLRRAVLDPAPLLQNGAGLLLWLIVALITGLWMTGKGSPN
                     IMMVFSASVVTANYTLKTPIWRILPLTRAELGQAQWWRLLGVPVLYASSSLALAAAAA
                     MAFGALKTPWPAMLAYGGAELTLIALFGAAMLLSAALAAPFGVVGYAAGLGLPLCAAF
                     VLGVRWIDRDLFAAHQGEMLAGGAAALALIGLLYLIGPRLPLARP"
BASE COUNT          196 a          512 c          589 g          267 t
ORIGIN      
        1 cggagcaggg cagggggctc gcggtcggag gagacggtta tcgatgttaa acaaagaggg
       61 gagcggcgga tgacgccagc gatcgtgacg gaaaaactct atgccagaca agggcgtttc
      121 gcgctcggtc cgctggattt cagcgcgccg cgcggcgcga tcacggccct gatcgggccg
      181 aacggggcgg gcaagacgac gttgctcgac ctgctgatgg ggctggggcg ggcgcaatcg
      241 ggccggatcg agctgctggg cctgacgcag ccgcgcgacg acgccgcgat caaggcgcgc
      301 gtcgcctatg tcaacccgga cctcaactat gcggtctggg gcacggtggg gcgggcgctg
      361 gatttcgtgc gcggcttcta tcccgactgg gacgaggcgc ggtgcgcgcg gctgctgacg
      421 gcgttcgaac tggcgcgcgg ccagaagatc gccgccctgt cgttcggcgc gcgcatcaag
      481 ctggccctga cgatggcgct ggcgcgcgag gccgagctgc tgctgctgga cgagccgacg
      541 atcgggctgg acgtcaacgc gcggcgggtg ctgtttcatg agttgctgga ggtggtgaag
      601 gccgagaacc gcgccgtggt catctcctcg catcaactca gcgacctcga gcggctggcc
      661 gatcactgcg cgatcctcgc caatggccgt ctggcggcca tggcgccgat ggacggcctc
      721 ctggcgcgct atgttcaggt cgacgccgtc tgcgccggac ggttgcccga aacgcccggc
      781 gcgcggctga tcgcgcgcaa cggcgaccgc gtgcgcctgc tgctggagcg cgcccgctgc
      841 gatgaggccg cgctggcggc gcagggcgtg cgcgtcgtgg ccgaggttcc gctcaatctc
      901 gaagacctgt tcgtggccct gaccgccgaa ccgctggagg ccgccgcatg atgtcgcctc
      961 gtctgcgccg tgccgtcctc gatcccgcgc cgttgttgca gaacggcgcc gggctgcttc
     1021 tctggctgat cgtcgccctg attacgggcc tgtggatgac cggcaagggt tcgccgaaca
     1081 tcatgatggt cttttcggcc tcggtcgtga ccgcgaacta tacgctcaag acgccgatat
     1141 ggcgcatcct gccgctgacg cgcgccgagt tggggcaggc gcaatggtgg cgcctgctgg
     1201 gcgtccccgt cctgtacgcg tcgtcgtcgc tggccctggc cgccgcggcg gcgatggcgt
     1261 tcggggcgct gaagacgccg tggcccgcga tgctggccta tggcggcgcc gaactgaccc
     1321 tgatcgccct gttcggcgcg gcgatgctgc tgtcggcggc gctggcggcg ccgttcggcg
     1381 tcgtcggcta tgcggcgggt ctgggcctgc cgctttgcgc cgccttcgtc ctgggcgtcc
     1441 gctggatcga ccgcgatctg ttcgcggccc atcagggcga gatgctggcg ggcggcgcgg
     1501 cggcgctggc gctgatcggc cttctgtacc tgatcggccc gcggctgccg ctggcgcgtc
     1561 ccaa
//
LOCUS       DASVLL010000560         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_91226, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000560 DASVLL010000000
VERSION     DASVLL010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91226"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VF402_09245"
     CDS             <1..340
                     /locus_tag="VF402_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEX7800522.1"
                     /translation="ALRGAAGSVIVGNPDIADVNVIDSHTLYIIGKGPGASAVTVTDG
                     AGRPLFDGEVVVTEAQRDAITVYHGLKASSVVCANVCVSADDAGGGASPGGAAMAASA
                     PAAQMTMNAQ"
     gene            375..911
                     /locus_tag="VF402_09250"
     CDS             375..911
                     /locus_tag="VF402_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7800523.1"
                     /translation="MGLGHLIRRGLTRRWADDRRGAAALEFALVIGPLIFMIMCCLEL
                     ALVILATVTLDNATDVAARQIRTGEVTSASTPDDFKALICDNMSWLAGGCLNSLEVDV
                     RTFDSFSLVDLTPPIKNGKFEASTFYYQIGGGSKIQLVRAYYEWPLFTPFLKPGLSTL
                     ANGDAMLVATDVFRNEPF"
     gene            911..1486
                     /locus_tag="VF402_09255"
     CDS             911..1486
                     /locus_tag="VF402_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7800524.1"
                     /translation="MLRRLNLLMRLKAFADDRRGVSALEFAFVAPILIMLYLGLAELT
                     LGMMASRRTAHLAATIGDLAAQSESLTTANIDDLFDIGDNLLAPFDTGTNLKIILSSV
                     KMAKDKNGAEKATVTWSVPYHATAHQPGDLVPAVTTTQISDGESLIMTEVEYDYDSPI
                     GHFLPGQTAFTYTYYHHPRNGSEVACTDCGS"
     gene            1486..1974
                     /locus_tag="VF402_09260"
     CDS             1486..1974
                     /locus_tag="VF402_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800525.1"
                     /translation="MARAAGFAILALWLAAVPVHAQDAPPRQLVSMSFYRGACHGLTT
                     PDGDFSEACAPALIMNLYSDGDTGYGFIHVDPGSHAQSAVSFLGGGAGMAPGSIAVEG
                     VILNAADGDKSFAARGVCTRAGPDDGAQSVSCEATTDDGMYTATFVSDGSPPQVRDMK
                     GK"
     gene            complement(2118..>3623)
                     /locus_tag="VF402_09265"
     CDS             complement(2118..>3623)
                     /locus_tag="VF402_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7800526.1"
                     /translation="PGGAFDHRAGNLPIVLFFFLDGHPALRAMAVNLLLVSGAMVMIL
                     LVYARDFERLVASQAETRRLSEDNFRIANCDSLTDLANRRWFFAELDKTLAAAQADNK
                     GFAVGIIDLDGFKPVNDTYGHTTGDRVLAEAAQRLRAACDDHITLARLGGDEFGFIYA
                     GNPNAAALTRLGEAITEAIRQPFTIGASNALLGASIGVACYPQASRDAEGLFERADYA
                     LYQVKRRQRGQMLIFSAAHESEVRAHGAIEQALQNADLDAELSLAFQPIVNVDSGETT
                     AFEALARWTSPSLGEIGPHAFIPVAERVGMIRQVTKTLLGKALAALRSWPEEVRLSFN
                     LSAHDLSDAETAVQIIALINRSGVNPRRLDFEITETAIVNDTRQSRAAAITFKALGVG
                     ISLDDFGTGYSSLSHVHKLPLDKLKIDRSFVTDIESNGASLKIVKSLIALCADMGLTC
                     IVEGVETHAQLRVLQSLGCRAAQGYYFARPMSGGDVANWLMRDGQAAGAGI"
BASE COUNT          606 a         1174 c         1242 g          601 t
ORIGIN      
        1 cgcgctgcgc ggggcggccg gatcggtgat cgtcggcaat cccgacatcg ccgacgtcaa
       61 tgtcatcgat tcccacacgc tctatataat cggcaagggg ccgggcgcca gcgcggtgac
      121 ggtcaccgac ggcgccgggc ggccgctgtt cgacggcgag gtcgtggtga ccgaggcgca
      181 gcgcgacgcg atcacggtct atcacgggct gaaggcgtcc agcgtggtat gcgccaatgt
      241 ctgcgtcagc gccgacgatg ccggcggcgg cgcgtcgccg ggcggcgccg cgatggccgc
      301 gtcggcgccg gcggcccaga tgaccatgaa cgcgcaatag atttcgggcg gcggcaaagg
      361 ggagcggcgc agcgatgggt ctgggccatc ttatccggcg gggactgacc cggcgatggg
      421 cggacgaccg gcgcggcgcg gcggcgctgg agttcgcgct ggtgatcggg ccgctgatct
      481 tcatgatcat gtgctgtctg gagctggctc tggtcattct ggccacggtg acgctggaca
      541 acgccaccga cgtggccgcg cggcagatcc gcaccggcga ggtgaccagc gcttcgacgc
      601 ccgacgattt caaggcgctc atctgcgaca atatgagctg gctggccggc ggctgcctca
      661 actcgctgga ggtcgacgtg cgcaccttcg acagcttctc gctggtcgat ctgacgccgc
      721 cgatcaagaa cggcaagttc gaggccagca ccttctatta ccagatcggc ggcggctcca
      781 agatccaact ggtgcgcgcc tattacgaat ggccgctgtt cacgccgttc ctcaagccgg
      841 gcctgtcgac cctggccaat ggcgacgcga tgctggtcgc cactgacgtc ttccgcaacg
      901 agccgttctg atgctgcgcc gcctcaacct cctgatgcgc ttaaaggctt tcgccgacga
      961 ccggcgcggg gtgtcggcgc tggaattcgc cttcgtggcg ccgatcctga tcatgctgta
     1021 tctggggctg gccgaactga cgctgggcat gatggcgtcg cgccgcaccg cgcatctggc
     1081 cgcgaccatc ggcgatctgg ccgcgcagtc ggaaagcctg accacggcca atatcgacga
     1141 cctgttcgat atcggcgaca atcttctggc gccgttcgac accgggacca atctgaagat
     1201 catcctcagc agcgtgaaga tggccaagga caagaacgga gcggaaaagg cgaccgtgac
     1261 ctggagcgtg ccctatcacg ccacggcgca tcaaccgggc gatctggttc cggcggtgac
     1321 gacaacgcag atttccgacg gcgaaagcct gatcatgacc gaggtggagt acgattacga
     1381 ttcgccgatc ggccatttcc tgccgggcca gacggcgttc acctatacct attaccatca
     1441 tccccgcaac ggctcggaag tcgcctgcac ggattgcggc tcctgatggc gcgcgcggcg
     1501 gggttcgcca tactggcgct gtggctggcc gccgttcccg ttcacgcgca ggacgcgccg
     1561 ccgcgtcaac tggtcagcat gagcttctat cgcggcgcct gccacggcct gaccacgccg
     1621 gacggcgatt tcagcgaggc ctgcgcgccc gcgctgatca tgaacctcta ttccgacggc
     1681 gataccggtt acggcttcat ccatgtcgat cccggcagcc atgcgcagtc ggcggtgtcg
     1741 tttctgggcg gcggcgcggg catggcgccg ggcagcatcg cggtcgaggg cgtgatcctg
     1801 aacgcggcgg acggcgacaa gagcttcgcc gcgcgcggcg tctgcacccg cgccggtccc
     1861 gacgacggcg cgcaaagcgt cagttgcgag gcgacgaccg acgacggcat gtacacggcc
     1921 accttcgtct cggacggatc gccgccgcag gtgcgggata tgaaggggaa gtagcggggg
     1981 ctgcgcgctg tcccccctcc gtcatttgct tcgcaaatgc cacctccccc gtgaacaggg
     2041 gaggatgagg ttattttatc cctccctgcg cagcggggag ggggaacttc aacctgtcat
     2101 taaaaggagg ggaccgctta aatgcccgcc cccgccgcct ggccatcgcg catcagccag
     2161 ttggcgacgt cgccgccgct catcgggcgg gcgaaataat agccctgcgc cgcgcggcag
     2221 cccaggcttt gcagcacgcg caactgcgcg tgcgtctcca cgccctcgac gatgcaggtc
     2281 aggcccatat cggcgcacag cgcgatcagc gacttgacga tcttgaggct ggcgccgttg
     2341 ctttcgatgt cggtgacgaa ggagcggtcg atcttcagct tgtccagcgg cagcttgtgc
     2401 acatggctga gcgaggagta gccggtgccg aaatcgtcga gcgagatgcc gacgcccagc
     2461 gccttgaagg tgatggcggc ggcgcgcgac tggcgcgtgt cgttgacgat ggcggtttcg
     2521 gtgatctcga aatcgaggcg gcgcggattg acgccggaac ggttgatcag cgcgatgatc
     2581 tgcacggcgg tttcggcgtc ggacaggtcg tgcgccgaca ggttgaacga caggcgcacc
     2641 tcctccggcc atgagcgcag cgccgccagc gccttgccca ataacgtctt ggtcacctga
     2701 cggatcatgc cgacgcgctc ggccaccggg atgaaggcgt gcgggccgat ctcgcccagg
     2761 ctggggctgg tccagcgcgc cagcgcctcg aaggcggtcg tctcgccgct gtcgacattg
     2821 acgatgggtt ggaacgccag cgacagttcg gcgtccagat cggcgttctg caaagcctgt
     2881 tcgatggcgc cgtgggcgcg cacctcgctt tcgtgcgcgg cggagaagat cagcatctgg
     2941 ccgcgctggc ggcgcttgac ctgatagagc gcgtagtcgg cgcgctcgaa caggccctcg
     3001 gcgtcgcgcg aggcctgcgg atagcaggcg acgccgatcg aggcgcccag aagcgcgttc
     3061 gaggcgccaa tggtgaaggg ctggcggatg gcctcggtga tcgcctcgcc cagacgggtc
     3121 agcgccgccg cgttcggatt gccggcgtag atgaagccga actcgtcgcc gccgagccgc
     3181 gccagggtga tgtgatcgtc gcacgccgcg cgcagccttt gcgccgcctc ggccagaacg
     3241 cggtcgccgg tggtgtggcc ataggtgtcg ttgaccggct tgaagccgtc cagatcgatg
     3301 atgccgaccg cgaagccctt gttgtcggcc tgcgccgccg ccagcgtctt gtccagctcg
     3361 gcgaagaacc agcggcggtt ggccagatcg gtcaggctgt cgcagttggc gatgcggaaa
     3421 ttgtcctccg acaaccgccg cgtctcggcc tgcgaggcga ccagccgctc gaaatcgcgc
     3481 gcatagacca gcaggatcat caccatcgcg ccggacacca gcaaaaggtt caccgccatc
     3541 gcgcgcaggg ccggatggcc gtccagaaag aagaacagca cgatgggcag attgccagcg
     3601 cggtgatcaa aagcgccgcc ggg
//
LOCUS       DASVLL010000561         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_147942,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000561 DASVLL010000000
VERSION     DASVLL010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147942"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VF402_09270"
     CDS             complement(<1..1086)
                     /locus_tag="VF402_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800527.1"
                     /translation="MSVKTLVSITAMAAALAVAGAAPAQETGPTKNQPAADQKKPGTG
                     NSSPDRGVLFEPTSSGSDGSVTVEGNRVVYHAVAGTLIVHPEGWDDAAWREKSSNDLG
                     DKKDAKAEASMFYVAYFKKGVPSADRPITFLYNGGPGSSTVWLHMGAFGPRRVITAND
                     SHTPAAPYNVVNNAFSLLDASDLVFIDAPGTGFSRIAGKDKEKAFLGVDADGHAFSEF
                     IKGFLSQYNRWNSPKYLFGESYGTPRSAVVINELETGDDIDFNGVILLSQILNFALSV
                     DGIEANPGSDTAYLTALPTYAATAWHYKLVPNAPADLTAFLKEVEHWTVTDYAQALLA
                     GSELDPSRKRAVAEKYAAYTGLPVDYVL"
     gene            1249..1497
                     /gene="infA"
                     /locus_tag="VF402_09275"
     CDS             1249..1497
                     /gene="infA"
                     /locus_tag="VF402_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX7800528.1"
                     /translation="MAKEELLEMRGQVVELLPNAMFRVRLENDHEILGHTAGKMRKNR
                     IRVLVGDEVLVELTPYDLTKGRITYRFMPGRGGPGPQG"
BASE COUNT          249 a          481 c          495 g          279 t
ORIGIN      
        1 cagcacatag tccacgggca ggccggtgta ggccgcgtac ttctccgcga ccgcacgctt
       61 gcgggacggg tcgagctcgg agccggccag cagcgcctgg gcgtagtcgg tgacggtcca
      121 gtgctccacc tccttcagga aggcggtcag gtcggccggc gcgttcggga ccagcttgta
      181 gtgccaggcg gtcgccgcat aggtcggcaa ggcggtgaga taggccgtgt cgctcccggg
      241 gttggcctcg atgccgtcca cggagagcgc gaagttcagg atctgcgaga gcaggatcac
      301 gccgttgaaa tcgatatcgt caccggtttc cagctcgttg atcaccaccg ccgagcgcgg
      361 cgtgccatag ctttcgccga acagatattt gggcgaattc cagcgattat attgcgacag
      421 gaaacccttg atgaactcgc tgaaggcatg gccatcggca tcgacgccca ggaacgcctt
      481 ctccttgtcc ttgccggcga tgcggctgaa gccggtgccc ggcgcgtcga tgaacaccag
      541 atcggacgcg tcgagcaggc tgaacgcgtt gttgacgacg ttgtaaggcg ccgccggggt
      601 gtgcgagtcg ttggcggtta tcacgcgccg cgggccgaac gcgcccatgt gcagccacac
      661 ggtcgaggag cccggaccgc cattgtagag gaacgtgatc ggccggtcgg cggacggcac
      721 gccctttttg aaataggcga cgtagaacat cgaggcctcg gccttggcgt ccttcttgtc
      781 gcccaggtcg ttcgaggatt tctcgcgcca ggcggcgtcg tcccagcctt cgggatggac
      841 gatcagcgtg ccggcgacgg cgtgataaac gacccggttg ccctccaccg tgacgctgcc
      901 gtcgctgccg ctgctcgtcg gctcgaacag cacgccgcga tcgggcgagg aattgccggt
      961 gccgggcttc ttctggtcgg cggcgggctg gttcttggtc ggcccggttt cctgcgcggg
     1021 ggcggcgccc gccaccgcca gtgcggcggc catcgccgtg atgctcacca aagtcttcac
     1081 gctcatgctc cctttcctcg tcccgggggg cggaccttag cgcggaaagt gtcttacgca
     1141 actgcaccac caccgaaaaa tcgcctttgg ctccggcgct tcctttttcc gcctcgttcc
     1201 gctaagggcg cgcatccttt cggctcaacc gaaaaccaag aggtctgttt ggcaaaagaa
     1261 gaacttctcg aaatgcgcgg gcaggtggtg gagctgctgc ccaacgccat gttccgcgtc
     1321 cggctggaaa acgaccatga gatcctcggt cacaccgccg gcaagatgcg caagaatcgc
     1381 atccgcgtgc tggtcggcga cgaggtgctc gtcgagctta cgccctacga tctgaccaag
     1441 gggcgcatca cctatcgctt catgccgggc cgcggcgggc cggggccgca aggctgatct
     1501 tccc
//
LOCUS       DASVLL010000562         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_307079,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000562 DASVLL010000000
VERSION     DASVLL010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307079"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VF402_09280"
     CDS             complement(<1..393)
                     /locus_tag="VF402_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800529.1"
                     /translation="MSFRFGAHGVFRRWALLVVFGAAAAAANAAGAQTFYGPQPYYRP
                     YGEPSFWREPAARRAAVAAVLREEGYRLWGPPEFSGRDVIALGVDEEGRKMRFVIDAE
                     DFEIISARRVREQARRSDLGDDGFERPYG"
     gene            complement(626..>1819)
                     /locus_tag="VF402_09285"
     CDS             complement(626..>1819)
                     /locus_tag="VF402_09285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800530.1"
                     /translation="YLDRVNVGFAALTMNDELGLSPTVYGWGAGVFFFGYCLLEAPSN
                     HILERVGARRWIARIMVSWGIVSAMMGLVWNETSFLVLRFLLGAAEAGFSPGVILFLT
                     YWVPARYRARSVGLFLLALPLATAVGAPLSGLLLSTMDGVGGASAWRWLFFIEAAPAI
                     LLGVAAYFYLTDKPQEARWLAPQERALLTRVLDQEKSSRKAAGAHPLWRMLGDPWVIR
                     LGVIYFGVVAALYGLGFWLPQIVSAFGYGPLGAGLLTAIPYACGGVAMALWSRRSDKN
                     GERVYHSAAAALLAAGGLAASAFFNAPIPSILALTAASVGTLAAMPPFWTLPTAFLCA
                     EVAAVGIALVNSIGNLAGFFGPYLVGWIKETSNDFSYALLALSAAPLFSAVLLVWLIP
                     EQKRR"
BASE COUNT          388 a          602 c          584 g          245 t
ORIGIN      
        1 gccatagggg cgctcgaagc catcgtcgcc gagatccgag cggcgcgcct gctcgcggac
       61 gcggcgcgcg ctgatgattt cgaaatcctc ggcgtcgatg acgaagcgca tcttgcgtcc
      121 ctcctcgtcg acgccaagcg caatgacgtc gcgccccgaa aactccggcg ggccccagag
      181 ccggtaaccc tcctcgcgca ggaccgccgc cacggcggcg cgccgcgccg ccggctcacg
      241 ccagaacgag ggctccccat aggggcggta atagggctga ggcccgtaaa aggtctgcgc
      301 gcccgccgca ttcgccgccg cggcggcggc gccgaaaacg acgagcaaag cccatcggcg
      361 aaacacgcca tgagcgccga atctgaaact catctcgcat gctcctcaag cttcccccgc
      421 catcgtcaag ctctgcgcgc aatgcggcgg ccttttgacc aaagccgagc tttgccgggg
      481 cgaagaaaat atccacgtga ggccgcgcgc cggacgcaag ccgcttccga gaccagcgga
      541 agacggtcga aatccgtatc ggctcaatga gacgcccaag ttatgaattt tctgttcacc
      601 ataattttgg ctcgaagtct tttccctatc ttcgcttttg ctcggggatg agccagacca
      661 gcaggacggc ggagaagagc ggcgccgcgg agagggcgag cagcgcatag gagaagtcgt
      721 tggacgtctc cttgatccag ccaacgagat agggtccgaa aaaaccggcg agattgccga
      781 tcgaattaac gagcgcaatg ccgaccgccg cgacctctgc gcaaagaaag gcggtcggga
      841 gcgtccagaa gggcggcatc gccgcaagcg ttccaaccga agccgccgtg agcgcaagaa
      901 tcgaggggat cggcgcgttg aagaaggcgg acgccgcaag gcctcccgcg gcgaggaggg
      961 cggcggcggc gctatgatag actctctcgc cgtttttatc ggaacgccgg ctccatagcg
     1021 ccattgcgac gcccccgcag gcgtagggga tggcggtcag caatcccgcg ccaagcgggc
     1081 cgtagccaaa ggcgctcacg atttgcggca gccagaagcc gagcccgtac agcgcagcca
     1141 cgacgccgaa ataaatgacc ccgaggcgga tcacccacgg atcgccgagc atccgccaga
     1201 gcggatgcgc cccggcggct ttgcggcttg atttttcctg gtccaacacg cgcgtcagca
     1261 aagcccgttc ttgcggcgcg agccagcgcg cttcctgcgg cttgtcggtg agataaaaat
     1321 aggctgcgac gccgagcagg atcgccggcg ccgcctcgat gaagaagagc cagcgccagg
     1381 cgctcgcgcc gcctacgccg tccatcgtcg aaagcaagag acccgaaagg ggcgcgccca
     1441 ccgccgtggc cagcggcagc gccagcagga agaggcccac gctgcgggcg cgatagcggg
     1501 ccggaaccca ataggtgaga aaaaggatca ccccgggcga gaaaccggct tccgcagcgc
     1561 cgagcaagaa ccgcagcacg aggaagctcg tctcgttcca gacaagcccc atcatggcgg
     1621 agacgatccc ccagctgacc atgatgcggg cgatccagcg ccgcgcgccg acgcgctcga
     1681 gaatatggtt gctcggcgcc tcgagcaggc aatagccgaa gaagaagacg cccgcgcccc
     1741 atccatagac ggtcggggaa agacccagtt cgtcattcat cgtcagcgcg gcgaagccga
     1801 cgttcacgcg gtcgagata
//
LOCUS       DASVLL010000563         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_36704, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000563 DASVLL010000000
VERSION     DASVLL010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36704"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /gene="rmuC"
                     /locus_tag="VF402_09290"
     CDS             complement(<1..1273)
                     /gene="rmuC"
                     /locus_tag="VF402_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX7800531.1"
                     /translation="MNMLPLSLLLVSLLALAFAGAWWLAFSESRRLRERADEMNDRLL
                     DVTNQLAAAKERARNLEDARAAMSEQFQIVSQQAMQASNEALMKAAAEREKLALERVG
                     HTLKPVAEQLTRFEAQVKAMEEARAKDTGGLKQQIHELMTASNATREVTQKLAGALRR
                     GAGVQGRWGEEVLRNVLQAAGLSARFDFLEQFSLDTEEGRRRPDVVVRLPGDASAVFV
                     IDAKVNLTHYLQAAETTDEAARDLLLQQHARAVQGHVRDLSVKAYWDQFRDQGSPDFV
                     AMFVPGDGLLAAALDHAPDLMTEAMDKKVIIVTPTTLFALCKAVAYGWRVEDQMKNAN
                     HIADLGRELYARLAVMGGHVGALGKSLGSAVEKYNDFVASLESRVLSKAREFEVYQVD
                     HQGKDLPEAKAIESQPRTANKLNSLQSTGEGA"
     gene            1433..1921
                     /locus_tag="VF402_09295"
     CDS             1433..1921
                     /locus_tag="VF402_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEX7800532.1"
                     /translation="MLIPFINWCWAAVPTVPLPDHGEWTPMAVVTGLVLVVRDFAQRE
                     IGHWIFIPLVIAIAASFYTSPSNIALASAVAFAISETVDWGLFTFLKLPLSKRVFISA
                     GLGAPIDTTVFYLMASATIAGIFNIYAIGSSIASKLVGCLIVYLLMKHRERKAALAAE
                     PA"
     gene            complement(2275..2874)
                     /gene="recR"
                     /locus_tag="VF402_09300"
     CDS             complement(2275..2874)
                     /gene="recR"
                     /locus_tag="VF402_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7800533.1"
                     /translation="MSGAGPEIERLMALLAKLPGLGPRSARRAALALLKRRDQLLIPL
                     TAAMRDAAEQVTTCGACGRLATRDPCETCADPARDSSMICVVEDDGSLWAMERVGAFH
                     GRYHVLGGLLSALDGVRPEDLRLDRLIARVQAAEVREVVMALPATVEGQTTAHYIADR
                     LKGLSNRIEVTYLARGVPVGGDLDWLDDNTIAHAFKTRR"
     gene            complement(2878..3207)
                     /locus_tag="VF402_09305"
     CDS             complement(2878..3207)
                     /locus_tag="VF402_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7800534.1"
                     /translation="MDFQQMMKQASMVQQKLQEAQARMHDSVVTGVSGGGMVSMELKG
                     AGDMTALSIDPSLMTPGEGEVLADLIRAAYADARRKLDDINTQLMRDAAASMGPGAVL
                     PDLPKFF"
     gene            complement(3207..>4851)
                     /locus_tag="VF402_09310"
     CDS             complement(3207..>4851)
                     /locus_tag="VF402_09310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7800535.1"
                     /translation="PRSFADLIGQEAMVRTLRNAFATGRIAHAFMLTGVRGVGKTTTA
                     RLLARALDYESDTVHQPSVDLSRMGVHCQAIIEGRHIDVLELDAASRTGVDAMRDLLD
                     SVRYAPVEARYKVYVIDEVHMLSTGAFNALLKTLEEPPPHAKFIFATTEIRKVPVTIL
                     SRCQRFDLRRVEPETLMPHLEKICGLEGVRIEPDALALIARAAEGSVRDSLSLLDQAL
                     VQGEPGEAVGAEVVRDMLGLADRSATLSLFENVISGQMSEALLAFRTLYGYGADPSQV
                     MGDLLEYCHATSVAKVLGAEATRLPNAQAMRVTALGAQLSAGTLSRLWTLMLKAFEEL
                     RRAPDPCAAFEMALVRMCYAADLPGPEALLKRLQNGEPLMPGAPNAPGGGAGGPTAMG
                     AAAVRMQAAPSFPNPQSFGEVLQLISDKRDIGLRADVERFVRLVAFRPGAITFEPAPN
                     APGDLVRKLSLRLKEWTGQRWLIATEGSGGAESIMERDKRETAESKARIQAHPFVAQV
                     MEIFPGAEITAIRARPKPVVEDAAPVAPEDERPDTDEDD"
BASE COUNT          794 a         1692 c         1525 g          840 t
ORIGIN      
        1 acgcgccttc gccggtcgat tgtaatgaat tcagtttgtt agccgtgcgc ggctggctct
       61 cgatcgcctt cgcctcgggc aggtccttgc cctgatgatc gacctgatag acctcgaact
      121 cgcgcgcctt gctcagcacg cggctctcca gcgaagcgac gaaatcattg tatttctcga
      181 ccgccgaccc cagcgacttg cccagcgcgc cgacatgccc gcccatcacc gccagccgcg
      241 catacagctc gcgccccaga tcggcgatat ggttggcgtt cttcatctgg tcctcgacgc
      301 gccagccgta ggcgaccgcc ttgcataggg cgaacagggt ggtcggggtg acgatgatca
      361 ccttcttgtc catcgcctcg gtcatcaggt cgggcgcgtg atccaacgcc gccgccagca
      421 gcccgtcgcc gggcacgaac atggcgacga aatcgggcga gccctgatcc ctgaactggt
      481 cccagtacgc cttgaccgac aggtcgcgca catggccctg cacggcgcgc gcatgttgtt
      541 gcagcaaaag atcgcgcgcg gcttcgtccg tcgtttcggc ggcctgcaga taatgggtca
      601 gattgacctt ggcgtcgatg acgaacaccg ccgaggcgtc gccgggcagg cgcaccacca
      661 cgtccgggcg gcggcggccc tcctcggtgt cgaggctgaa ctgctccaga aaatcgaagc
      721 gcgccgacag gccggccgcc tgcaacacgt tgcgcagcac ctcctcgccc cagcgcccct
      781 gcacgcccgc gccgcgccgc aaggccccgg ccagcttctg cgtcacctcg cgcgtggcgt
      841 tcgaggcggt catcagctcg tgaatctgtt gtttcagccc gccggtgtcc ttggcgcgcg
      901 cctcctccat cgccttgacc tgcgcctcga agcgcgtgag ctgctccgcc acgggtttca
      961 gcgtgtgacc gacgcgctcc agcgccagtt tctcgcgctc ggcggcggcc ttcatcagcg
     1021 cttcgttcga cgcctgcatc gcctgctgcg acacgatctg gaactgctcg ctcatggcgg
     1081 cgcgcgcgtc ctccagattg cgggcgcgct ccttcgccgc cgccaattga ttggtcacgt
     1141 ccagcagccg gtcgttcatc tcgtccgcgc gttcgcgcag gcggcggctt tccgaaaagg
     1201 ccagccacca cgcgccggcg aaggccaggg cgagcagcga aaccaacagc agactcagcg
     1261 gcaacatgtt catgttacgt tcctatgccg tccgcgcctt gctgacaagt cttttcttgc
     1321 ggcccgtcca taaagcggta aattcaggat ttcggagggc ggcatgacgg attttcgcac
     1381 ctttgcgcgc tagaccttca ccgggcgcgc gccgtggacc tatctctata tcgtgctgat
     1441 tccgttcatc aactggtgct gggcggcggt gccgaccgtg cccctgcccg atcacggcga
     1501 atggacgccg atggcggtcg tgaccgggct ggtgctggtg gtgcgcgact tcgcgcagcg
     1561 cgagatcggc cactggatct tcattccgct ggtcatcgcc atcgcggcct ccttctacac
     1621 ctcgccgtcc aatatagcgc tggccagcgc ggtcgccttc gcgatctcgg agacggtgga
     1681 ctgggggttg ttcaccttcc tgaaactgcc gctgtcgaag cgggtgttca tctcggcggg
     1741 cctcggcgcg cccatcgaca ccaccgtctt ctacctgatg gcctcggcga cgatcgcggg
     1801 gattttcaac atctacgcta tcggctcgtc gatcgcgtcc aagttggtcg gctgcctgat
     1861 cgtctatctg ctcatgaagc atcgcgaacg caaagcggcg ctggcggcgg aacctgccta
     1921 ggcgccgaac agcgtaaaaa cgaagacgaa gataaacata tagcgcaaag gcatggcgaa
     1981 ctcctccgcc gccagcctta aagcgttaag agtttgttaa ccagccctaa ccgcccgtta
     2041 cgcgaaaaat gccggtaaag accgagacgg atcggggcaa agcaagggtt tttatccaca
     2101 gatgaacaca gatattccgg gtcatcacac gctaccgcta tcgccagtgg aagcatgata
     2161 aaggcgcttc gcgccaaatg acgtcaagcg gcaggctcga tagctagcgg tagcgtggaa
     2221 tcaccatgaa tatctgtgtt catctgtgga taaaaccttc tacctaaagg tccgtcagcg
     2281 ccgcgttttg aaagcatgcg caatcgtgtt gtcatccagc cagtcgagat cgccgcccac
     2341 cggcacgccg cgcgccagat aggtgacctc gatgcggttg ctcagacctt tcagacggtc
     2401 ggcgatatag tgggccgtgg tctggccctc gaccgtggcg ggcagggcca tcaccacctc
     2461 gcgcacctcg gccgcctgca cgcgcgcgat caaccggtcg aggcgcaaat cctcaggccg
     2521 caccccgtcc agcgccgaca gcagcccgcc cagcacatga tagcggccgt gaaaggcgcc
     2581 gacgcgctcc atcgcccaca gcgagccgtc gtcctcgacc acgcagatca tgctggaatc
     2641 gcgcgccgga tcggcgcagg tctcgcacgg atcgcgcgtc gccagccgcc cgcacgcgcc
     2701 gcaggtcgtg acctgctccg ccgcgtcccg catggcggcg gtcagaggaa tcaacagttg
     2761 atcgcgccgc ttgagcaagg ccagggccgc ccgccgcgcc gaacgcggtc caagaccggg
     2821 cagcttggcc aacagcgcca tcaggcgctc gatttccggt ccggcgccgc tcaagtctta
     2881 aaagaacttc ggcaggtcgg gcagcaccgc gcccggcccc atcgacgccg ccgcgtcgcg
     2941 catcagctgc gtgttgatgt cgtccagctt cctgcgcgca tcggcgtagg cggcgcggat
     3001 caggtcggcc agcacctcgc cctcgcccgg cgtcatcagc gacggatcga tgctgagcgc
     3061 ggtcatgtcg ccggcgcctt tcagctccat cgacaccatg ccgccgccgg aaacgccggt
     3121 cacgacgctg tcgtgcatcc tggcctgcgc ctcctgcagc ttctgctgca ccatcgaggc
     3181 ctgtttcatc atctgctgaa agtccatcag tcgtcctcat ccgtatcggg gcgttcgtct
     3241 tcgggcgcga caggcgcggc gtcctcgacc accggtttcg ggcgcgcgcg gatcgcggtg
     3301 atctcggcgc cggggaaaat ctccatgacc tgcgcgacga acgggtgcgc ctggatgcgc
     3361 gccttcgatt cggcggtttc gcgcttgtcg cgctccatga tcgattcggc gccgccggag
     3421 ccttcggtgg cgatcagcca gcgctggccc gtccattcct tcaggcgcaa ggacagcttg
     3481 cgcaccagat cgccgggcgc gttgggcgcc ggctcgaagg tgatggcgcc gggccggaac
     3541 gcgaccagcc gcacgaagcg ctcgacatcg gcgcgcaggc cgatatcgcg cttgtccgag
     3601 atcagttgca gcacctcgcc gaagctctgc ggattgggaa agctgggcgc ggcctgcatc
     3661 cgcacggcgg cggcccccat cgccgtcggt ccgcccgcgc cgccgcccgg cgcattcggc
     3721 gcgcccggca tgagcggctc accgttttgc aggcgcttca gcaaggcttc cggccccggc
     3781 aggtcggcgg cgtaacacat gcgcaccagc gccatctcga aggcggcgca cggatcgggc
     3841 gcgcggcgca gttcctcgaa cgccttgagc atcagggtcc acaaccggct caacgtcccg
     3901 gcggatagtt gcgcgcccag cgccgtcacg cgcatggcct gcgcgttcgg cagccgcgtc
     3961 gcctcggcgc ccagcacctt ggccaccgag gtcgcgtggc agtattccag caagtcgccc
     4021 atgacctgcg acggatcggc gccgtagccg taaagcgtgc ggaaggccag cagcgcctcg
     4081 ctcatctggc cggaaatcac gttctcgaac aggctcagcg tcgcggaccg gtcggccagg
     4141 cccagcatgt cgcgcaccac ctcggcgccg accgcctcgc ccggctcgcc ctgcaccagc
     4201 gcctgatcga gcagggacag cgagtcgcgc accgagcctt ccgccgcgcg cgcgatcagg
     4261 gccagggcgt ccggctcgat gcgcacgcct tcgaggccgc agatcttctc cagatgcggc
     4321 atcagggttt ccggctcgac gcggcgcagg tcgaagcgct ggcagcgcga caggatggtg
     4381 accggcacct tgcggatttc ggtcgtggcg aagatgaact tggcgtgcgg cggcggctct
     4441 tccagcgtct tcagcagcgc gttgaaggcg ccggtggaca gcatgtgcac ttcgtcgatc
     4501 acatagacct tgtagcgcgc ctcgaccggc gcatagcgca ccgaatcgag caggtcgcgc
     4561 atggcgtcga cgccggtgcg cgaggccgcg tccagctcca gcacgtcgat atggcggcct
     4621 tcgatgatcg cctgacaatg cacgcccatc cggctaagat cgacgctggg ctgatgcacc
     4681 gtgtcgctct cgtaatcgag cgcgcgcgcc agcaagcggg ccgtggtcgt cttgccgacg
     4741 ccgcgcactc cggtcagcat gaaggcgtgg gcgatgcggc ccgtggcgaa ggcgttgcgc
     4801 agggtgcgca ccatcgcctc ctggccgatc aggtcggcga aggaccgcgg c
//
LOCUS       DASVLL010000564         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_413121,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000564 DASVLL010000000
VERSION     DASVLL010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413121"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF402_09315"
     CDS             <1..193
                     /locus_tag="VF402_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800536.1"
                     /translation="EVQDARGDWRKLAEQAEGQDTAAVRAVETEAREGRKVRIRLRAP
                     AGYPAGLAELRVSGALHTD"
     gene            complement(207..1538)
                     /gene="dgt"
                     /locus_tag="VF402_09320"
     CDS             complement(207..1538)
                     /gene="dgt"
                     /locus_tag="VF402_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEX7800537.1"
                     /translation="MDWSKLLKAARLAAPDYQEQPGRPAHMVDIDRITFSAPFRRLAN
                     KTQVHPLYDNDHVHHRLIHSVEVANVGRSLGMLVGQWLVDTGEVDAAARDRISGVVQA
                     ACLAHDIGNPPFGHSGEAAIGSWFAERFEAPRGVVSELAAADRQEFTAFEGNAQGFRI
                     LTRTEMSVNRGGMRLSLGVLGAFTKYPCAAHVSARRPEAYCGLKKFGVFNNDISTFKE
                     IAEALELPFAGNAAAPWWRRHPLVFLMEAADDICYNIMDLEDAFISGDVAFEQARALL
                     SALFAQPNREYAPKSEAAEIAVLRAIAVHNAIDACVEAFKTHYDALMAGTFDGSLIEA
                     SSKAAEFAAIKDLARARIFKAARKTELEVFGRNVIYRALDGLIPLLDDLAAKNWDASR
                     LSGYHQQLARAVNLDLNGVDNAYDALHVLTDFVSGMTDRYAVKTAEMLYGR"
BASE COUNT          297 a          569 c          529 g          290 t
ORIGIN      
        1 cgaggttcag gatgcgcggg gcgactggcg aaagctcgcc gaacaggccg agggccagga
       61 cacggccgcg gttcgcgccg tcgaaaccga ggcgcgtgaa ggccgcaagg tgcgcattcg
      121 cctgcgcgcg cccgcaggtt atcccgcggg cctcgccgaa cttcgggtca gcggcgcgct
      181 gcatacggac tgagagggcg tccgggctac cggccgtaga gcatttcggc ggtcttgacc
      241 gcatagcggt cggtcatgcc ggagacgaaa tcggtcagga cgtgcagcgc gtcataggcg
      301 ttgtcgacgc cgttcaggtc gagattcacc gcccgcgcaa gctgctggtg atagcccgac
      361 aagcgcgacg cgtcccagtt cttcgccgcc agatcgtcca gcagcgggat cagcccgtcc
      421 agcgccctgt agatcacgtt gcgcccgaac acctcaagct cggtcttgcg cgccgccttg
      481 aagatgcgcg cccgcgccag atccttgatg gcggcgaatt cggcggcctt gctcgacgcc
      541 tcgatcagcg agccgtcgaa cgtcccggcc atcaaagcgt cgtaatgggt cttgaacgcc
      601 tcgacgcagg cgtcgatggc gttgtggacg gcgatggcgc gcagcaccgc gatctcggcc
      661 gcctcggact tcggcgcata ttcgcggttg ggctgcgcga acagggccga cagcagcgcc
      721 ctcgcctgct cgaacgcgac gtcgccgctg ataaaagcgt cctccaagtc catgatatta
      781 tagcagatat cgtcggcggc ctccatcagg aacacaagcg gatgccgccg ccaccagggc
      841 gccgccgcat tgccggcgaa cggcaattcc aatgcttcag ctatttcctt gaacgtactg
      901 atatcattat tgaaaacgcc gaacttctta agtccgcaat aagcttcggg gcggcgggcg
      961 ctgacatggg ccgcgcacgg atatttcgtg aaggcgccca gcacgccgag gctgagccgc
     1021 atgccgccgc gattgacgct catctcggtg cgggtcagga tgcggaagcc ctgcgcattg
     1081 ccctcgaagg cggtgaattc ctgccggtcc gccgccgcca gttcgctcac aacgccgcgc
     1141 ggcgcctcga agcgctccgc gaaccagctt ccgatcgccg cctcgcccga atggccgaac
     1201 ggcggattgc cgatgtcgtg cgccagacag gccgcctgca cgacgcccga tatgcggtcg
     1261 cgcgccgccg cgtccacctc gcccgtgtcc accagccact gcccgaccag catgccgagc
     1321 gagcgcccga cattggccac ttcgacggaa tggatcagcc ggtgatgcac atggtcgttg
     1381 tcgtaaagcg gatgcacctg cgtcttgttg gccagccgcc ggaacggcgc cgagaaggtg
     1441 atgcggtcga tatcgaccat atgggcgggc cgccccggct gctcctgata gtcaggcgcc
     1501 gccagccgcg ccgccttcag cagcttgctc cagtccacat cgctctcctg tatcggccat
     1561 caggccatgt ccgcgcaacg ccggttttag gcgttgcgcg gagtcgccgc aaggccgctt
     1621 gactcccgca ttgcgttgca atatataatt aggtagctaa ctaataatat cgttctcccc
     1681 atgac
//
LOCUS       DASVLL010000565         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_307227,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000565 DASVLL010000000
VERSION     DASVLL010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307227"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2260
                     /locus_tag="VF402_09325"
     CDS             <1..2260
                     /locus_tag="VF402_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800538.1"
                     /translation="NGSSRSFFYDKTPSSFLGTGTFVPSATNAPNAAVVQAMFASYGV
                     TSTVNPLLNLGFNNDGTLYVQTGAVNYRGPTNSQGYLIVAGNVRMPVGQQVDFYNGLQ
                     RKTAFLKADYEVTPGITLYGQFMYVDLTVKTASGNSLTQFGKLTTIPVTNPFIPANLR
                     TLLASRPNATAPFTWNGRYVGVPYKNWDENYKIQQYVGGIKGDIAPGWTFDGFFAYDK
                     SDHDQTLHFGIVKSKVQTLLNAADGGASLCAGGFNPFGDANARSLSAACVAYITKDAL
                     SIERLSQTQVQFQVNGKLFDLGAGPAQIAFVADYRRNTYSYVPDSDLQVQNIEAVIAS
                     APADGKISVKEAAAQIDIPLLADKPFFQEFGIGGAVRVSDYSTSGSVTSFEGDARWRP
                     IKEVLFRGSYQRAVRAPNIGELFSPASGTQLVIGTPPGSLGDPCDVRSTARTGANAAQ
                     VATLCVAQGIPAAAISSYTFPTTATGQTIAGNRGLTPERANTFNFGVVINAPRNDGPL
                     GDISLSVDYYKINIKNVISTVPGLTVLSKCFNLDGSNPTYSNTNSYCALIQRDASGQL
                     STVNTPYLNLGALKTDGIEIQFHYGLPARFISESGKLYVDSAVGYLRNYDVQLLPGAA
                     FLNYTGISNGAAGTGSVPPRATPTWKALTSFGYRSDAFSIGLRWRYQNAMKDVSSVLT
                     PATAAVGVPAYSIWDTFASVRVGKEFELRFGVNNLFDKGLPFVASSQNSTDTALYDPI
                     GRSFYAGAKFAF"
BASE COUNT          449 a          733 c          664 g          433 t
ORIGIN      
        1 caacggcagc agccggtcgt tcttctacga caagacgccg tcgtccttcc tcggcaccgg
       61 caccttcgtg ccgagcgcga cgaacgcgcc caacgccgcc gtcgtacagg cgatgttcgc
      121 gtcgtacggc gtgacctcaa cggtcaatcc gctgctcaac ctcggcttca acaatgacgg
      181 gacgctgtac gtccagacgg gcgcggtcaa ttatcgcggg ccgaccaact cgcagggcta
      241 tttgatcgtc gccggcaacg tccgcatgcc ggtcgggcag caggtcgatt tctacaacgg
      301 cctccagcga aagacggcct tcctcaaggc cgattacgaa gtcacgcccg gcatcacgct
      361 ctatggccag ttcatgtatg tcgacctgac cgtcaaaacc gccagtggca acagcctgac
      421 ccagttcggc aagctgacga cgatcccggt aacgaacccg ttcatccctg cgaacctgcg
      481 caccctgctg gcgtcgcggc ccaacgcgac cgcgccgttc acctggaacg gccgctatgt
      541 cggcgtaccg tacaagaatt gggacgagaa ctacaaaatc cagcaatatg tcggcggcat
      601 caagggcgac atcgcgcccg gatggacgtt cgacggcttc tttgcctacg acaagtcgga
      661 ccacgaccag acgctccatt tcggcattgt gaagagcaag gtgcaaacgt tgctcaacgc
      721 ggcggacggc ggtgcgtcgc tgtgcgccgg agggttcaat ccattcggtg acgcaaatgc
      781 gcgttcgctg tcggcggcgt gtgtcgccta tatcaccaag gatgcgctct cgatcgagcg
      841 cctgtcgcaa acccaggtgc agttccaggt caacggcaag ctgttcgatc tcggcgccgg
      901 cccggcacaa atcgcgttcg tcgcggatta tcgccgcaac acctacagct acgtccccga
      961 tagcgatctg caggtgcaaa atatcgaagc ggtgatcgcc tcggcgcccg ccgatggcaa
     1021 gatttcggtg aaggaggccg cggcgcagat cgacatcccg ctgctggctg acaagccgtt
     1081 cttccaggaa tttgggatcg gcggcgcggt gcgcgtgtcg gattactcga catcgggctc
     1141 cgtcaccagc ttcgaaggcg acgcgcgctg gcggccgatc aaggaggtgc tgtttcgcgg
     1201 cagttaccag cgcgcggtgc gtgcacccaa tatcggcgaa ctgttctcgc cagcatcggg
     1261 cacccagctc gtgataggca caccgccggg ctcgctcggc gatccgtgcg acgtccgttc
     1321 gaccgcccgc accggcgcca acgccgccca ggtcgccacg ctctgcgttg cgcagggtat
     1381 cccggcagcg gcgatcagta gctatacctt ccctactacg gcgaccggcc aaaccatcgc
     1441 aggcaatcgc ggactgacgc cggaacgcgc gaacacgttt aacttcggcg tggtgatcaa
     1501 tgcgccgcgc aatgacgggc cgctcggcga catttcgctg tcggtcgatt actacaagat
     1561 caacatcaag aacgtgatct cgaccgtgcc ggggctgacc gtgctgtcga aatgcttcaa
     1621 cctcgacggg tcgaacccga cctattcgaa tacgaacagc tattgcgcgc tcatccagcg
     1681 cgatgcgagc ggccagctat cgacggtcaa cacgccctat ctcaacctcg gcgcgctgaa
     1741 gaccgacggc atcgaaatcc aattccacta tggattgccc gccaggttca tctcggagtc
     1801 tggcaagttg tacgttgatt ctgccgtcgg ctaccttcgc aattacgacg tccagctcct
     1861 gccgggtgcg gcgttcctga actatacagg catcagcaat ggtgccgccg gcactggatc
     1921 agtgccgccg cgcgctacgc caacctggaa agcgctcaca tcgttcggtt atcgttcgga
     1981 cgcgttcagc atcgggctgc gctggcggta ccagaatgcg atgaaggacg tcagttctgt
     2041 gctgacgccg gcgacggcgg cggttggtgt gccggcttac tcgatctggg ataccttcgc
     2101 ctcagtgcgg gtcggtaaag aatttgagct tcgcttcggc gtaaacaacc tgttcgacaa
     2161 gggacttccg ttcgttgcga gctcccaaaa ttcaaccgac acagcactct atgacccgat
     2221 tggccgttcg ttctacgctg gggccaagtt tgcattttaa cacatgatag gacgctacg
//
LOCUS       DASVLL010000566         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_413156,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000566 DASVLL010000000
VERSION     DASVLL010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413156"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..738)
                     /locus_tag="VF402_09330"
     CDS             complement(25..738)
                     /locus_tag="VF402_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX7800539.1"
                     /translation="MRRILTGFFVVLCVALLTALGVWQVQRLAWKTDLIARVDARVHA
                     PPVPAPENWSAVTRATDEYRHVRVSGRFLNADESQVYTLSELGAGYWVMTPLRTDDGR
                     IVYINRGFVTEDKKAPASRPQGQTGAEVTVTGLLRMPETKGWLFQQRNDPAHDAWYRR
                     DIDQMAAAHGLGPVAPYFIDADDAPNPGGWPRGGLTVIHFPNSHLQYAITWFSLAILL
                     AGAAIWTTWFRKPDETDEA"
     gene            complement(791..2863)
                     /locus_tag="VF402_09335"
     CDS             complement(791..2863)
                     /locus_tag="VF402_09335"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7800540.1"
                     /translation="MTRFRQFATMKLATMKLAATALAAAAVSAAPIFAGSAFAASPID
                     GAADAFLRQSILTATGKAAIDARCQAALDLGNKAKTELEGHTGPATVAGDFQAFDTLS
                     LIVGDASNEMYLVSETNTAKDVRDAAEACLPKLSDLGTAVSLSRPIYDRLAAIPTQGL
                     DEKTAYIIGKTLTQYRLAGVDKDAATRARVADLQKAITTTGLQFDANIRDDTGDIALK
                     PDDLKGLPQDYIDAHKPGADGMVHITFAYPDIFPILDFADSRETRKKVSDAFRNRGYP
                     ANEATLRTLLQQRYDLARTLGYDDYAALITADKMIGSEQHAAQFLDDMNAAAAPGAKA
                     DYDELLTFAKTLDPSITQLQGYDNAYFANKLQKAKYDVDAAQVRQYFTLAKTRAGIFQ
                     LVHDLFGADIEPWDAPVWAPDVTAWSIYDGAGSQKRLVGHFYLDLSPRDGKYNHAAQF
                     PIRTGVEGRQVPVGALVTNFPATGPMDHDDVTTFLHEFGHLIHDMYSGHTQYASQSMN
                     SLQWDFIEAPSQLLEEWTWDYDTLKGFASDPQGRPIPEDLVKRMNQGRHFGEAIRWKT
                     QLAYSAVSLNFYNRAPDFDLTTMYNDQISRYSLFPPVPGTHPYASFGHLNGYSAIYYT
                     YVWSKAIALDLFTQFQKDGMRNPATALRYRQLVLDPGGSEDANDLIRNFLGRPMSLEA
                     FKDELQKK"
     gene            3065..>3309
                     /locus_tag="VF402_09340"
     CDS             3065..>3309
                     /locus_tag="VF402_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEX7800541.1"
                     /translation="MTTELLEGCTGVLVLSDGSVYQGLGCGATGEALGEVCFNTAMTG
                     YQEILTDPSYMEQIVAFTFPHVGNVGVNSEDVEQLGD"
BASE COUNT          543 a         1015 c         1119 g          632 t
ORIGIN      
        1 atccgccgtc cgcgcttttc gtggctaagc ctcgtccgtc tcgtccggtt tgcggaacca
       61 ggtcgtccag atcgcggcgc cggcgagcag gatggccagc gagaaccagg tgatcgcgta
      121 ttgcaggtgg ctgttcggga agtggatcac cgtcaggccg cccctgggcc agccgccggg
      181 attgggggcg tcgtcggcgt cgatgaaata gggcgcaacc ggccccaagc catgcgccgc
      241 cgccatctga tcgatgtcgc ggcggtacca ggcgtcgtgc gcggggtcgt tgcgctgctg
      301 gaacagccag cctttggttt cgggcatgcg cagcaggccc gtcaccgtga cctcggcgcc
      361 ggtctggccc tgcgggcggg acgcgggcgc tttcttgtct tcggtgacga agccgcgatt
      421 gatatagacg atgcgcccgt catcggttcg cagcggcgtc atcacccaat agccggcgcc
      481 gagttcgctc aacgtataga cctgtgattc gtcggcgttg aggaagcggc cgctgacgcg
      541 cacatggcgg tattcgtcgg tcgcgcgcgt caccgcgctc cagttctccg gcgccggaac
      601 cggcggggcg tggacgcgcg catcgacccg cgcgatcagg tcggtcttcc acgccagcct
      661 ctgcacctgc cagacgccga gcgcggtcaa cagcgcgacg cacaggacga cgaaaaatcc
      721 ggtcagaata cggcgcatgg cgtgtctcct atcgggaagg ccgtatggac cttcccggca
      781 ggcggcgcaa ttatttcttc tgaagttcgt ccttgaacgc ctccagactc atcgggcggc
      841 cgaggaagtt gcggatcaga tcgttggcgt cctccgaacc gcccggatcc agcacgagct
      901 ggcgatagcg cagggcggtc gccgggtttc tcatgccgtc cttctggaac tgcgtgaaca
      961 ggtcgagcgc gatcgccttg gaccagacat aggtgtagta gatcgccgaa tagccgttca
     1021 ggtggccgaa gctggcgtaa ggatgcgtgc ccggcacggg cgggaacagg gaatagcggc
     1081 tgatctggtc gttatacatc gtcgtcagat cgaaatcggg cgcgcggtta tagaagttga
     1141 gcgacaccgc cgaataggcc agttgcgtct tccagcggat cgcctcgccg aaatggcgcc
     1201 cctgattcat gcgcttgacg aggtcttcgg ggatcggacg gccctgcgga tcggaggcga
     1261 agcctttgag cgtatcgtaa tcccaggtcc attcctccag cagttgcgac ggcgcctcga
     1321 tgaagtccca ttgcaggctg ttcatcgatt gcgaggcgta ttgcgtatgg ccggaataca
     1381 tgtcgtggat caggtggccg aactcatgca ggaaggtggt cacgtcgtca tgatccatcg
     1441 gccccgtcgc cgggaagttg gtgaccagcg cgccgaccgg aacctgacgg ccctcgacgc
     1501 cggtgcggat cgggaactgg gcggcgtgat tgtacttgcc gtcgcgcggg ctgagatcga
     1561 ggtagaagtg cccgaccagg cgtttctggc ttccggcgcc gtcgtagatc gaccacgccg
     1621 tcacgtccgg cgcccagacc ggcgcgtccc acggctcgat gtcggcgccg aacaggtcgt
     1681 gcaccagctg gaagatgccc gcgcgcgtct tggccagggt gaaatactga cgcacctgcg
     1741 cggcgtcgac gtcgtacttc gccttttgca gcttgttggc gaagtaggca ttgtcatagc
     1801 cctgcaattg cgtgatcgac ggatcgagcg ttttggcgaa ggtcagcaat tcgtcgtaat
     1861 cggccttggc gccgggcgcg gcggcggcgt tcatgtcgtc gaggaactgc gcggcgtgct
     1921 gctccgagcc gatcatcttg tcggcggtga tcagcgcggc gtaatcgtcg tagccgagcg
     1981 tcctggccag atcgtagcgc tgctgcaaca gcgtcctgag cgtcgcctcg ttggccggat
     2041 agccgcggtt gcggaaggcg tccgacacct tcttgcgcgt ctcgcggctg tcggcgaagt
     2101 cgaggatcgg gaagatgtcg ggataggcga aggtgatgtg gaccatgccg tcggcgccgg
     2161 gcttgtgcgc gtcgatatag tcctgcggca gccccttcag gtcgtcgggt ttcagcgcga
     2221 tgtcgcccgt gtcgtcgcgg atattggcgt cgaattgcag gcccgtggtg gtgatcgcct
     2281 tttgcagatc ggcgaccctg gcgcgcgtgg ccgcgtcctt gtccaccccg gccagcctgt
     2341 attgagtcag ggtcttgccg atgatgtagg cggtcttttc atccagcccc tgcgtcggaa
     2401 tcgccgccag ccggtcgtag atcggccgcg acagcgacac cgccgtgccg aggtcggaca
     2461 gtttcggcag gcaggcctcc gccgcgtcgc gcacgtcctt ggcggtgttg gtttccgaca
     2521 ccagatacat ttcgttggac gcgtcgccga cgatcaggct cagcgtgtcg aaagcctgaa
     2581 aatcgcccgc gaccgtcgcc gggccggtat ggccttccag ctcggtcttg gccttattcc
     2641 ccagatcgag cgccgcctga cagcgcgcgt cgatggcggc cttgccggtc gcggtcagga
     2701 tcgattgccg cagaaaggcg tcggccgcgc cgtcgatggg agaagcggcg aaggcggacc
     2761 cggcgaagat cggcgcggcg gaaacggccg cggcggcgag cgccgtggca gcgagcttca
     2821 tcgtggcgag cttcatcgtg gcgaactggc ggaatcgtgt catcgtgaac agccctatag
     2881 tttgcgatga aacgattggt ctcacatccc gtccgcccgc gcaaccgcga ttgctaacat
     2941 gacgtgatgg ccaaagacct gcgcgcgcgt gattagcaat tgactcgcag gtgcgcagtc
     3001 cctatgcgtg cggccgttta cccgcaagcc tgccctgacg cccctttgaa ggagatgacg
     3061 ccccatgacc actgaactgc tcgaaggctg cacaggggtt ctggtcctgt cggacggctc
     3121 ggtctatcag ggcctcggct gcggcgcgac cggcgaggcc ctgggcgagg tgtgcttcaa
     3181 caccgccatg accggctatc aggaaatcct gaccgatccg tcctatatgg agcagatcgt
     3241 cgccttcacc ttcccgcatg tcggcaatgt cggcgtcaac agcgaagatg tcgagcaact
     3301 gggcgacga
//
LOCUS       DASVLL010000567         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_469483,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000567 DASVLL010000000
VERSION     DASVLL010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469483"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..587
                     /locus_tag="VF402_09345"
     CDS             135..587
                     /locus_tag="VF402_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7800542.1"
                     /translation="MWLALIAAAFLLLVYVGRQSRLGRLRPGPWIRQFRAARSLIGTA
                     LCVFGVSLLVRGLVWEGAAAIVAGIVMMGTVRVQAAFRRPEPQVAAAYTEAEIRAYRT
                     LDLAVGADRNAIKAAWKRLVKESHPDRGGSLERAKALNAAKDLLLRRR"
     gene            584..1366
                     /locus_tag="VF402_09350"
     CDS             584..1366
                     /locus_tag="VF402_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7800543.1"
                     /translation="MTPDTILHLEKLALAAWPAQETAHVHGWALRAAQGFTKRANSAN
                     ALTPEGDFAALRAAAEAFYRARGLPPVFRLTPLAPPEADAALAAAGYQEADPSRVMTA
                     AVRKDSPPDVQVDEAVADGWLDGFAAANGVAGALRPLHDGMVRRIEGRTGFVTLRRDG
                     RALGYGLAVCAEGWVGLFDLVIASEARGQGLGRRLVDGLTAWGAAHGADRAYLQVRDG
                     NMAARRLYVRCGFSDAYAYRYRMGPPRALSEKCDALFGLKSA"
     gene            complement(1416..2888)
                     /locus_tag="VF402_09355"
     CDS             complement(1416..2888)
                     /locus_tag="VF402_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX7800544.1"
                     /translation="MRNFGRSFGRKISPLTSGAMAALLLASGVGAAAHAQDLADNAKY
                     AGIVVDAQTGEVLYGMRADAARYPASLTKIMTLYLVFDALSHGQIKPDDQIVISQHAA
                     SQAPVKVYLKPGDTIDVDTAMRLAAMYSANDMAVALAEKVGGTEERFAAMMTIKAQEL
                     GMTETRFVNANGLPDARQITSARDLAILARAVLRDYPQYYSYFDLPSVTFRGRTYYNH
                     NPLHGIPGVDGMKTGFTNAAGLTLVASQVKDGHRLIAVIMGASSSRQRREHGEELLNI
                     GFDVENRRDHGETLVAQAEFDKAFANGGVIPSGPQPYQAAPGAPVPYTMLASASQPLS
                     DQQLRDTLAGSEQANATDTDVQRASAVVTAPDVVAALNAAPTAAALPVRKAAPVKVAV
                     RTRLAGVETRKNHRKKNPEAVWSIQVGAFRDKALATDWVRKVQQRFDDALADGRSEVS
                     RAGGGMYRTRFAALTKEQAHSACKSMTARHLDCMVIKPDA"
     gene            3297..3734
                     /gene="phaP"
                     /locus_tag="VF402_09360"
     CDS             3297..3734
                     /gene="phaP"
                     /locus_tag="VF402_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079753.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEX7800545.1"
                     /translation="MVEAAETLKTTVEKMSAAGNQAYKDSVEKTLTALNEVSALSKSN
                     VEAVVESLTAATKGAETVGAQALAYSKKSWDDAVTAAKTLSGAKSVQEVIELQSKYAK
                     ASMEAYVAEMNQFAETLSASFKDTFKPINARVTATVEKFQSYR"
BASE COUNT          627 a         1214 c         1366 g          669 t
ORIGIN      
        1 ccggtcgcca tgtcgctcgg ccacgtctcg gcgcggcagg agctgcgcgc gctgatggcg
       61 tcgctggggc tggacgaaag cggcgacgac atgggcgtcc ccgccgatct cgaaaccgag
      121 gaggcgtaaa ggccatgtgg ctggccctga tcgcggcggc gttcctgctg ctggtctatg
      181 tcgggcgcca gtcgcgtctg ggacggctca ggccggggcc gtggatacgc cagttccgcg
      241 ccgcgcgcag cctgatcggc acggcgctgt gcgtgttcgg cgtaagcctg ctggtgcgcg
      301 gcctcgtctg ggaaggcgcg gcggcgatcg ttgccggcat cgtcatgatg ggcacggtgc
      361 gcgttcaggc cgcttttcgc cgcccggaac cgcaggtcgc ggcggcctat accgaggccg
      421 aaatccgcgc ctaccgcacg ctggacctcg cggtcggcgc ggaccgcaac gcgatcaagg
      481 cggcgtggaa gcggctggtc aaggagtcgc atcccgaccg cggcggctcg ctggagcgcg
      541 ccaaggcgct caacgcggcc aaggatctgc tgctgaggcg gcggtgacgc cggatacgat
      601 cctgcatctg gaaaagctgg cgctggcggc gtggcccgcg caggagacgg cgcatgtcca
      661 cggctgggcc ttgcgggcgg cgcagggctt caccaagcgg gccaattccg ccaacgcgct
      721 gacgcccgaa ggcgatttcg cggctttacg cgccgccgcc gaagcctttt accgcgcgcg
      781 cggcctgccg ccggtgttcc gcctgacgcc gctggcgccg ccggaggcgg acgcggcgct
      841 ggcggcggcg ggctatcagg aggccgatcc gtcgcgcgtg atgacggcgg cggtgaggaa
      901 ggactcgccg cctgatgtgc aggtcgatga ggccgttgcc gacggctggc tggacggttt
      961 cgcggcggcc aacggcgtgg ccggggcctt gcgtccgctg catgacggca tggtgcggcg
     1021 catcgaggga cgcacgggtt tcgtgactct gcgccgggat ggacgcgcgc tcggctacgg
     1081 tctggcggtg tgcgcggagg gctgggtcgg cctgttcgat ctggtgatcg catccgaggc
     1141 gcgcgggcag gggctggggc ggcgcctcgt cgacggcctg acggcgtggg gcgcggcgca
     1201 cggcgcggac cgcgcctatc tgcaggtgcg cgacggtaat atggcggcgc gccgcctcta
     1261 tgtccgctgc ggtttctcgg acgcctatgc gtatcgttac aggatggggc cgcctagagc
     1321 gctttccgaa aagtgtgacg cacttttcgg attaaaaagc gcgtaaaagc ataaagttag
     1381 agcgccgatc tgatgccatc agatcggaaa acgctctaag cgtccggctt gatgaccatg
     1441 cagtcgaggt gccgggccgt catcgacttg caggcgctgt gggcctgctc cttggtcagg
     1501 gcggcgaagc gggtgcggta catgccgccg cccgcgcggc tgacttccga gcggccgtcg
     1561 gccagcgcgt cgtcgaagcg ctgctgcacc ttcctgaccc agtcggtcgc cagcgccttg
     1621 tcgcggaagg cgccgacctg gatcgaccag acggcttcgg ggttcttttt ccggtggttc
     1681 ttccgggtct cgactccggc aaggcgcgtc ctgaccgcca cttttacggg cgcggccttc
     1741 ctgacgggca gggcggcggc ggtcggcgcg gcgttgagcg cggcgacgac gtcgggcgcc
     1801 gtcacgaccg cgctggcgcg ctgcacgtcg gtgtcggtgg cgttggcctg ctcgctgcct
     1861 gcaagcgtgt cacgcagttg ctggtcgctg agcggctggc tggcgctggc cagcatcgtg
     1921 taaggcacgg gcgcgccggg ggcggcctga tagggctgag gcccggacgg gatgacgccg
     1981 ccattggcga acgccttgtc gaactcggcc tgcgcgacca gggtttcgcc gtggtcgcgg
     2041 cggttctcga cgtcgaagcc gatgttcagc agctcctcgc catgctcgcg gcgctggcgg
     2101 ctggagctgg cgcccatgat gacggcgatc aggcggtggc cgtccttgac ctgcgacgcc
     2161 actagggtca ggcccgccgc gttggtgaag ccggtcttca tgccgtccac gcccggaatg
     2221 ccgtgcagcg gattgtggtt gtaataggtg cggccgcgga aagttacgct cggcaggtcg
     2281 aaatagctgt agtattgcgg atagtcgcgc aggaccgcgc gcgccagaat ggccaggtcg
     2341 cgcgccgagg tgatctggcg cgcgtcgggc aggccgttgg cgttgacgaa gcgcgtctcg
     2401 gtcatgccca gctcctgcgc cttgatcgtc atcatggcgg cgaagcgctc ctcggtgccg
     2461 ccgacctttt cggccagcgc cacggccatg tcgttggccg aatacatggc ggccagacgc
     2521 atggcggtgt cgacgtcgat ggtgtcgccg ggcttgagat aaaccttgac gggcgcctgg
     2581 gaggcggcgt gctgactgat gacgatctga tcgtcgggct tgatctggcc gtgcgacagc
     2641 gcgtcgaaca cgagataaag cgtcatgatc ttggtcagcg aggccgggta gcgcgcggcg
     2701 tcggcgcgca tgccgtacag cacctcgccg gtctgggcgt ccacgacgat gcccgcgtat
     2761 ttggcgttgt cggccagatc ctgcgcatgc gccgccgcgc cgacgccgga cgccagcagc
     2821 agcgcggcca tggcgccgga ggtgaggggc gagattttgc gcccgaacga acgcccgaaa
     2881 ttacgcatcg aagcctgatc cctaactcaa tccgcggaca ccgccgtctg ccgcgaccct
     2941 aaaatatcaa tgtggtccca ttatggagcg catggctcat gtggcaagta tgtcacggca
     3001 ttttcttaat caaactttaa tcacacctta ttgtgaactg cgccgcgatg gcggcggcgt
     3061 aaggcgaaag gcaggaaacc accaagcgcg cgttctggct aaaaaactcc cacctcaggg
     3121 gaatcgccgc atcacgatcg cgttaagctt ttgtgcggcg caacataatt cttgacctgc
     3181 ttttgcgact gcgatataac ggcgttcatc cgggcaggat gtccggcggt aaggaacatg
     3241 agacgttccc catgtcccgg cttgtccaat cctattcaaa tttaaggagc cttccaatgg
     3301 tagaagctgc tgaaaccctg aaaacgaccg tcgaaaagat gtccgccgcc ggcaatcagg
     3361 cgtacaagga ctcggtcgaa aagaccctga ccgcgctcaa cgaagtcagc gccctgtcca
     3421 agagcaatgt cgaggccgtg gtcgaaagcc tgaccgccgc gaccaagggc gccgaaaccg
     3481 tcggcgcgca ggcgctggcc tactccaaga agtcctggga cgacgcggtc accgccgcca
     3541 agaccctgtc cggcgccaag agcgtgcagg aagtgatcga gctgcaatcg aagtacgcca
     3601 aggcctcgat ggaagcctat gtcgccgaga tgaaccagtt tgccgaaacc ctgtcggcct
     3661 cgttcaagga tacgttcaag ccgatcaacg cgcgcgtcac ggcgacggtc gagaagttcc
     3721 agagctaccg ttaatccggt gtgaaggacg cgcgcttggc aagccgatca acgcgcgcgt
     3781 catcgccacg gtcgagaagt tccggagcta ccgttaatcc ggcctgctcc gatccgatcg
     3841 aaggccccgc gcaagacctg cgcggggcct tttcgc
//
LOCUS       DASVLL010000568         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_148229,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000568 DASVLL010000000
VERSION     DASVLL010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148229"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1348)
                     /locus_tag="VF402_09365"
     CDS             complement(155..1348)
                     /locus_tag="VF402_09365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7800546.1"
                     /translation="MAARGDSPKGPDRFYELDILRGLAAIIVVTFHYKHFLLISDRAG
                     FDYHDMPLHAILMPVYVYGQFFVELFFSISGYVFFWLYAEAIAARRIGLRGFFVARFA
                     RLYPLYFVTFIAVALIQWAFHSLYGHDYIYGGNTAVNFALNLVMAHQWLPHAVQNFNG
                     PSWSISVEVFLYAVFFLLCRFRLQSVWTVAALVVAGLFLRGLNPDVDFLRGLPSFFLG
                     GLVWYFVTELRRHDALRRRLAVALAAVLPVLWGLTYVWGYDSLWLRVTQALSRHGVHW
                     PPVTTVGAFCYVLIPLTLLFFGLQRGLWLKPERLKRFEWVGDISYSVYLIHFPLQISL
                     MLVLAQLPFPTRARIFSSPFAYLAFMAAAMTLAWLSYRYFETPMRKHIREWLAPRLAR
                     LRPVR"
     gene            complement(1349..>2062)
                     /locus_tag="VF402_09370"
     CDS             complement(1349..>2062)
                     /locus_tag="VF402_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7800547.1"
                     /translation="DSSSLRRGKVAAHLIWGAPSSVLVGDFLFARAFELMVETGSLRA
                     LGILSKASAVITQGEVMQLTKAFDLNLSRADYIEIISAKTAALFAAASESGAVAAGAD
                     DARVQALFDYGLNLGLAFQIRDDVLDYEGSADALGKNAGDDFAEGKSTLPLILAMRAT
                     ADTDAEFWERAIGRRQQTDGDFAHAQRLMRASGALDAARAAAETYADAARAALSVFAP
                     GPWRSALEALALYSVERKV"
BASE COUNT          429 a          672 c          680 g          281 t
ORIGIN      
        1 cggtgccggt cgcgcccgcg acgacgccgt gccggttggc gcgcccgtac agcaggattt
       61 cggacgattt atcggcgttt tgcgcctgcc ctacgaaagc gccactggcg tcggccatct
      121 gatagtctcc tgatgaagcg aactgacgga attcttaccg caccggcctg aggcgcgcca
      181 gtctgggcgc aagccattca cgaatgtgtt tacgcatcgg cgtctcgaaa taacggtagc
      241 tgagccaggc cagcgtcatc gccgccgcca tgaaggcgag ataggcgaag ggcgaggaaa
      301 aaatccgcgc gcgcgtcgga aaaggcaatt gcgccagcac cagcatcagg ctgatctgca
      361 acggaaaatg gatcaggtac accgaatagc tgatgtcgcc gacccattcg aagcgtttca
      421 ggcgttccgg cttcagccac agaccgcgtt gcaggccgaa aaacagcagg gtcagcggga
      481 tcagcacgta acagaaggcg cccaccgtgg tgacgggcgg ccaatgcacg ccgtgacggc
      541 tgagcgcctg cgtgacgcgc agccacagcg aatcgtagcc ccacacatag gtcagccccc
      601 acaacaccgg caggaccgcc gccagagcga ccgccagacg ccggcgcaag gcgtcgtggc
      661 ggcgcagctc cgtcacgaaa taccagacga ggccgcccag gaaaaagctc ggcaggccgc
      721 gcaggaaatc gacgtcgggg ttcagcccgc gcaggaacag gcccgcgacg accagcgccg
      781 cgaccgtcca gacggattgc aggcggaaac ggcacaacag gaagaacacg gcgtacagga
      841 acacctcgac cgagatcgac cagcttgggc cgttgaagtt ctgcaccgcg tgcggcagcc
      901 actgatgcgc catgacgagg ttgagcgcga aattgaccgc ggtgttgccc ccgtagatgt
      961 agtcgtgccc gtacaggctg tggaacgccc actggatcag ggcgacggcg atgaaggtga
     1021 cgaaatagag cggatacagc cgggcgaaac gcgcgacgaa aaagccgcgc agcccgatcc
     1081 ggcgcgccgc gatcgcctcg gcgtagagcc agaagaagac atagccggag atcgagaaga
     1141 acagctcgac gaagaactgg ccatagacat agaccggcat gagaatcgca tgcagcggca
     1201 tgtcgtgata gtcgaaaccg gcgcggtccg aaatcagcag gaagtgcttg taatggaagg
     1261 tgacgacgat aatcgcggcc agcccgcgca ggatgtccag ctcgtaaaag cggtccggcc
     1321 ccttcgggct gtccccccgc gccgccacct agaccttgcg ctcgacgctg tacagggcca
     1381 gcgcttccag cgccgaacgc cacggaccgg gcgcgaagac ggacagcgcc gcgcgcgccg
     1441 cgtccgcata ggtttcggcg gccgcgcgcg ccgcgtccag agcgccgctg gcgcgcatca
     1501 ggcgctgcgc gtgcgcgaaa tcgccgtcgg tctgctggcg ccggccgatg gcgcgctccc
     1561 agaattcggc gtcggtatcc gccgtcgcgc gcatggccag gattagcggc agggtggact
     1621 tgccctcggc gaagtcgtcg cccgcattct tgcccagcgc gtcggccgag ccttcgtagt
     1681 cgagtacgtc gtcgcggatc tggaaggcga ggccgagatt caggccgtaa tcgaagagcg
     1741 cctgcacgcg cgcgtcgtcc gcgcccgccg ccaccgcgcc gctttccgac gccgccgcga
     1801 acagcgccgc cgtcttggcc gagatgattt cgatatagtc ggcgcgcgac aggttgagat
     1861 cgaacgcctt ggtcagttgc atcacctcgc cctgggtgat caccgccgag gctttcgaca
     1921 ggatgcccag cgcgcgtaag gagccggtct cgaccatcag ttcgaaggcg cgcgcgaaca
     1981 ggaaatcgcc gaccagcacc gaggacggcg cgccccagat caggtgcgcg gccaccttgc
     2041 cgcgccgcag cgacgaggaa tc
//
LOCUS       DASVLL010000569         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_148249,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000569 DASVLL010000000
VERSION     DASVLL010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148249"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VF402_09375"
     CDS             <1..772
                     /locus_tag="VF402_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX7800548.1"
                     /translation="AGGAAMAVAAAAQAQNAGQNNSEAENVQKALEDFNGPELNVTPV
                     GEDWANLHRYHVANAGAAALPDAQRRVVMLGDSITDNWAKPQFSDSFYADNGLIGRGI
                     SGQVSAQMLVRFMADVIALKPRAVHIMAGTNDIGENKDPYDPVATTNNLQAMISLARL
                     FGLKVLLGSVTPATSFPWRPSIGNPHDKIVALNNWLKSVATAQDCVYVDYWPALATPD
                     GGLKPELGINGDAVHPNKDGYAQMQPILLAAVGKALAG"
     gene            822..>2132
                     /locus_tag="VF402_09380"
     CDS             822..>2132
                     /locus_tag="VF402_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEX7800549.1"
                     /translation="MKTGLATVAALFAVAFPALACEGPVAVCDGAGPGVALVRGGRPV
                     AVYVDADAEKPVARVAGDFAADLGRVSGTDAAIVHSLDGAPGDVVVIGEIGHSRLIDG
                     WITAGRLDVSAIRGQWEAYAQQVIDLPGGRHALVITGADKRGAIYGTYDISAKMGVSP
                     WYWWADVPTRHKDDLYVTAGLRHDQPVVKYRGFFINDEEPSFGGWAREKFGGINHQLY
                     AKVFELDLRLKGNFLWPAMWGKAIADDDPQSLTTADDYGVVLGTSHHEPMTRAQEEWH
                     RHTDQGVTGGAWDYTKNAANLDTFWRGGVERMVKGRHEDVVTIGMRGDGDEPMSEGTA
                     TQLLETIVADQRRIIADVTGKPASETPQVWALYKEVQDYYDHGMKVPDDVTLLFSDDN
                     WGQIRRLPKFDAPERAGGYGIYYHFDYVGGPRNYKWINTNQIEKT"
BASE COUNT          390 a          663 c          736 g          343 t
ORIGIN      
        1 ggcgggcggg gcggctatgg ccgtcgcggc cgccgctcag gcgcaaaacg cgggccagaa
       61 caacagtgaa gccgaaaatg ttcagaaagc gctcgaagat ttcaacgggc ctgaactgaa
      121 cgtcacgccg gtgggcgagg actgggcgaa cctgcaccgc taccacgtcg ccaacgccgg
      181 cgccgccgcc ttgccggacg cgcagcgccg cgtcgtcatg ctgggcgatt cgatcaccga
      241 caactgggcc aagccgcagt tttcggacag cttctacgcc gacaacggcc tgatcgggcg
      301 cggcatttcc ggtcaggtgt cggcgcagat gctggtgcgc ttcatggccg acgtgatcgc
      361 gctcaagccc agggccgtgc atatcatggc cgggaccaac gacatcggcg aaaacaagga
      421 tccttacgat ccggtcgcca cgaccaacaa tctgcaggcc atgatcagcc tggcgcggct
      481 gttcgggctg aaggtgctgc tcggctccgt gacgcccgcg accagcttcc cgtggcgtcc
      541 ctcgatcggc aatccgcacg acaagatcgt cgcgctcaac aactggctga agtcggtcgc
      601 gacggcgcag gactgcgtct atgtcgacta ctggccggcg ctggcgacgc ccgacggcgg
      661 tctcaagccg gaactgggca tcaacggcga cgccgtgcat cccaacaagg acggctacgc
      721 gcagatgcag ccgatcctgc tggccgcggt cggcaaggct ctggcgggtt aattccgctg
      781 cggacatggc ggcggcgttt tttcccaagc gacttcatct gatgaaaacc ggtcttgcga
      841 ccgtcgcggc cctgttcgcc gtcgcgtttc cggccttggc ctgcgaaggg cctgtggcgg
      901 tctgcgacgg cgccgggccg ggcgtggcgc tggtccgcgg cggcaggccg gtcgcggtct
      961 atgtcgacgc ggacgccgaa aagccggtgg cgcgcgtggc gggcgatttc gccgccgatc
     1021 tgggccgcgt gtcgggaacg gacgccgcca tcgtccacag cctcgacggc gcgccgggcg
     1081 acgtcgtggt gatcggcgag atcggtcatt cgcgcctgat cgacggctgg atcacggcgg
     1141 gcaggctcga cgtctcggcc attcgcggac agtgggaggc ttacgcccag caggtgatcg
     1201 acctgccggg cgggcgtcat gcgctggtga tcacgggcgc ggacaagcgc ggcgccatct
     1261 acggaaccta cgacatctcc gcgaagatgg gcgtgtcgcc gtggtactgg tgggcggatg
     1321 ttcccactcg gcacaaggac gatctctacg tcacggcggg gctgcgccac gatcagccgg
     1381 tggtgaagta tcgcggcttc ttcatcaatg acgaggagcc gtccttcggc ggctgggcgc
     1441 gcgaaaagtt cggcgggatc aatcaccagc tttacgccaa ggtgttcgag ctggacctgc
     1501 gcctgaaggg gaatttcctg tggcccgcca tgtggggcaa ggcgatcgcg gacgacgatc
     1561 cgcaaagcct gaccacggcg gacgattacg gcgtcgtgct gggaacctcg caccacgagc
     1621 cgatgacgcg cgcgcaggag gaatggcatc gtcataccga tcagggcgtg accggcggcg
     1681 cgtgggatta caccaagaac gccgccaacc tcgatacgtt ctggcgcggc ggcgtcgagc
     1741 gcatggtgaa ggggcgtcat gaggacgtgg tgaccatcgg catgcgcggc gacggcgacg
     1801 aaccgatgag cgagggcacg gcgacgcaat tgctggaaac catagtcgcc gaccagcgcc
     1861 ggatcatcgc tgatgtgacc ggcaagccgg cttcggaaac gccgcaggtc tgggcgctct
     1921 acaaggaggt gcaggattat tacgaccacg gcatgaaggt tcccgatgac gtgactctac
     1981 tgttctcgga cgacaactgg ggccagattc gccgtctgcc gaagttcgac gcgccggaac
     2041 gcgcgggggg ctatgggatc tattaccatt tcgactatgt cggcgggccg cgaaactata
     2101 agtggatcaa caccaaccag atcgaaaaga cc
//
LOCUS       DASVLL010000570         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_523188,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000570 DASVLL010000000
VERSION     DASVLL010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523188"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2411
                     /locus_tag="VF402_09385"
     CDS             <1..2411
                     /locus_tag="VF402_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX7800550.1"
                     /translation="NEIHPDYYQIPVADREKLMAELAQAQSKGNGDRAEAHGDDAEDD
                     DHHHDDEGDDLPDEERRLRQLLSRRYKIQEVIKRRQILLVQVVKEERGNKGAALTTYL
                     SLAGRYCVLMPNTARGGGISRKITNATDRKRLKTMVSSLDVPHGMGLIVRTAGAKRTK
                     QEIKRDYDYLLRVWENIRENTLKSNAPALIYEEEDLVKRAIRDLFDKDFDGVLVEGED
                     GYRSAKDFMRMIMPSQAKKIQLYKANAPMFARYGVEDMISKIYSPVVPLKSGGYLVIN
                     QTEALVAIDVNSGKSTKERNIEATALKTNMEAAEEAARQMRLRDLAGLVVIDFIDMDE
                     PKNNRSVEKKLKDALAEDRARIQMGKISGFGLMEISRQRRRTGFLEGTTVVCPHCEGT
                     GRIRSVDSAALSAMRAIDMEAMQNGAGAVTIKLPVAVALYVLNEKQVHLAKLRLEWGL
                     KVNVEIDADMGQTDIQIVRTVHADEVDFVPPVRPEPIAFDPVSDDEIEDETDDEAEVE
                     REDTDDDEASARVEDQSDRDGDRRGRRRRRRRGGKDRDRPENEERAAGEDDGEGESED
                     EGEEDDNGFRKGRRRRGRRGGRRSGFDGRPRETYSWVRARTPSLEAPYVWIDPFDEQP
                     RAQNDASRNDASRHDAARAETPAVVNESVSVLGEATTQPTSLTPDDVAAAPAPKRPRP
                     TRRRKAAPEAESADAPAEPVAETAPVEAPAEPVAEAPLEEAPAKPARRTRSRKKTAEA
                     EPAPEAAAPEAIVAETAPTPVTAKALETVAVAPALPEPAPSEPDAAEISAPPEKPKRG
                     WWRR"
BASE COUNT          502 a          875 c          842 g          333 t
ORIGIN      
        1 tcaatgaaat ccaccccgac tactatcaga tcccggtcgc cgaccgcgaa aagctgatgg
       61 ccgaactggc gcaggcccag tcgaagggca atggcgaccg cgccgaggcc cacggcgacg
      121 acgccgagga cgacgatcac caccacgacg acgaaggcga cgacctgccc gacgaggagc
      181 gccgcctgcg ccagctcctg agccgccgct acaagattca ggaagtcatc aagcgccgcc
      241 agattctgct ggtccaggtc gtcaaggagg agcgcggcaa caagggcgcc gccctgacca
      301 cctacctgtc gctggccgga cgctattgcg tgctgatgcc gaacacggcg cgcggcggcg
      361 gcatttcgcg caagatcacc aacgcgaccg accgcaagcg gctgaaaacc atggtgtcct
      421 cgctggacgt gccgcacggc atgggcctga tcgtgcgcac cgccggggcc aagcgcacca
      481 agcaggagat caagcgcgac tacgactacc tgctgcgcgt gtgggagaat atccgcgaaa
      541 acacgctgaa atcgaacgcg cccgcgctga tctacgagga ggaggatctc gtcaagcgcg
      601 cgatccgcga cctgttcgac aaggatttcg acggcgtgct ggtcgagggc gaggacggct
      661 accgttccgc caaggacttc atgcgcatga tcatgccgtc gcaggcgaag aagatccagc
      721 tctacaaggc caacgccccg atgttcgcgc gctacggcgt cgaggacatg atctcgaaga
      781 tctattcgcc ggtcgtgccg ctgaaatcgg gcggctatct ggtcatcaac cagaccgagg
      841 ctctggtcgc catcgacgtc aactccggca agtcgacgaa agagcgcaat atcgaggcga
      901 ccgcgctcaa gaccaatatg gaggcggccg aggaagccgc gcgccagatg cgtctgcgcg
      961 atctggccgg gctggtggtc atcgacttca tcgacatgga tgagccgaag aacaaccgtt
     1021 cggtcgaaaa gaagctcaag gacgcgctgg ccgaggaccg cgcccgcatc cagatgggca
     1081 agatttccgg cttcggcctg atggagatca gccgccagcg ccgccgcacc ggcttcctgg
     1141 aaggcaccac ggtcgtctgc ccgcactgcg aaggcacggg ccgcatccgt tcggtcgatt
     1201 ccgccgccct gtcggcgatg cgcgccatcg acatggaggc gatgcagaac ggcgccggcg
     1261 ccgtcacgat caagctgccg gtcgcggtcg cgctctacgt cctcaacgaa aagcaggtgc
     1321 atctggccaa gctgcggctg gaatggggcc tgaaggtcaa tgtcgagatc gacgccgaca
     1381 tgggccagac cgacatccag atcgtgcgca ccgtccatgc cgacgaggtc gatttcgtgc
     1441 cgcccgtgcg gcccgaaccg atcgccttcg acccggtgag cgacgacgag atcgaggacg
     1501 agaccgacga cgaagccgag gtcgagcgcg aggatacgga cgacgacgaa gcctcggccc
     1561 gcgtcgagga tcagtccgat cgcgacggcg accgtcgcgg acgccgccgt cgccgccgtc
     1621 gcggcggcaa ggaccgcgac cgccccgaga acgaggagcg cgccgccggt gaagacgacg
     1681 gcgaaggcga aagcgaagat gaaggcgaag aggacgacaa cggcttccgc aagggccgcc
     1741 gccgccgcgg ccgccgcggc gggcgccgct ccggtttcga cggccgtccg cgcgaaacct
     1801 atagctgggt gcgcgcgcgc acgccgtcgc tggaggcgcc ctatgtctgg atcgatccgt
     1861 tcgatgagca gccgcgcgcc cagaatgacg cttccaggaa cgacgcttcc aggcatgacg
     1921 ccgccagagc ggagacgccc gcagtcgtaa acgaatccgt cagcgtgctg ggcgaggcca
     1981 cgacgcagcc gacctcgctg acgccggacg atgtcgccgc cgcgcccgcg ccgaagcgtc
     2041 cgcgtccgac gcgccgccgc aaggccgcgc cggaagccga aagcgccgat gcgcccgccg
     2101 aaccggtcgc ggaaaccgcc ccggtcgagg ctccggcgga gccggtcgcg gaagctcccc
     2161 ttgaggaagc gcccgccaag cccgcccgcc gcacgcgcag ccgcaaaaag acggccgaag
     2221 ccgaaccggc gccggaagcc gccgcgcccg aagcgatcgt cgcggaaacc gccccgacgc
     2281 ccgtgacggc gaaggcgctg gagaccgtcg ccgtcgcgcc cgccttgcct gagcccgctc
     2341 cgtccgagcc cgacgcggcg gaaatttccg ccccgccgga aaaaccgaaa cgcggctggt
     2401 ggcgccgcta agcatgaaaa ggcgggcctt cgggtccgcc tttttctttc gcgccgcgcc
     2461 ctagatttgt cacgcttgcc gcttacatcg gatgggctgg cttactgtaa ccgcttgctg
     2521 taaggtcttt gcgatgcagg attttcgcgc ct
//
LOCUS       DASVLL010000571         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_307853,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000571 DASVLL010000000
VERSION     DASVLL010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307853"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF402_09390"
     CDS             complement(<1..499)
                     /locus_tag="VF402_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800551.1"
                     /translation="MKALVLMAAVASLAISPAAFADTSTDNTNINTDIASVCKVTSGA
                     SDVTLHTTVNDSGSHIGQISNASFDLTGNTMHVFCNTAGSQIAISRTKLTNQDVSTIS
                     AASQALGFTRELDYTAELSGNTETGLKTINSQGFLAPSSTTYGVYASDLTLSVAGLQL
                     LNWNKM"
     gene            complement(600..1178)
                     /locus_tag="VF402_09395"
     CDS             complement(600..1178)
                     /locus_tag="VF402_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800552.1"
                     /translation="MRRSGHSLVLALLAAALPVTAGAHALHGDSDSARVTLRARAPTA
                     CSVTPTTSSLEVASNAVATDRGAVFDFHAPPASRVSVSCNMASSQISVRAEAMQLLNG
                     RLSAASAHAGFTDMIDFAVTVTGPTAYAAAAQLGDGTSQATHPDGAVPDHIPYGAYAG
                     DMQLEVSRIAPRDGKTPVSGSYRGLISVTVSP"
     gene            complement(1175..1786)
                     /locus_tag="VF402_09400"
     CDS             complement(1175..1786)
                     /locus_tag="VF402_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800553.1"
                     /translation="MPGAAALILGCLISTVSYAHNHTHWPWDHNAPSGALPVFDGPEQ
                     GLDILVTGRVQEQCKFLDAPSPVNDVELQSGASLNIPFRVYCNSGFSVTLSSLHGGLA
                     SDADGLTARSRAAGFSDQASYSARFEVRRDDGSALSETCTSAQLHGGGAGCAMASGAG
                     LRSNVPAMNQSGYLAVTMANVDPSRLLAGSYDDVLTVNVQAQL"
BASE COUNT          420 a          644 c          669 g          404 t
ORIGIN      
        1 gcatcttgtt ccagttcagc agttggaggc ccgcaacgct cagcgtcagg tcgctggcgt
       61 acacgccgta ggtcgtggag ctgggggcga ggaagccctg gctgttgatg gtcttcaggc
      121 cggtttcggt gttgcccgac agttccgccg tatagtcgag ttcacgggtg aaacccagag
      181 cctgcgacgc ggccgagatg gtgctgacgt cctgattggt cagcttggtg cgcgagatgg
      241 cgatctgcga accggcggtg ttgcagaaca catgcatcgt gttgccggtc aggtcgaacg
      301 acgcgttcga gatctggccg atatggctgc ccgaatcgtt gacggtcgtg tgcagggtca
      361 cgtccgaagc gccgctcgta accttacaca ccgacgcgat gtcggtgttg atgttggtat
      421 tgtcggtcga cgtgtcggcg aaggccgcgg gggagatggc gaggctggcg acagcggcca
      481 tcaaaactaa cgctttcata actgatctcc tcaagagagt tggtgtttcg cctgcgctga
      541 gatacgcgcg ccgtccggac cgcgcagatg gcccggaagc gcttacaaaa ccgccgtccc
      601 tacggactga cagtgacgct gatcagcccc cggtaagacc ccgaaacggg cgtcttgccg
      661 tcgcggggcg cgatccggct gacctccagc tgcatgtcgc ccgcataggc gccataagga
      721 atgtggtcgg gaacggcgcc gtcgggatgc gtcgcctgcg acgtgccgtc gcccagctgc
      781 gccgccgccg cataggcggt cggtcccgtc acggtcaccg cgaaatcgat catgtccgta
      841 aagcccgcgt gcgccgaggc cgcgctgagc cgcccgttca gaagctgcat cgcctcggcg
      901 cggacgctga tctgcgagga cgccatgttg caggacacgc tgacgcggct ggccggcggc
      961 gcatggaaat cgaagaccgc cccccggtcc gtcgccacgg cgttcgacgc gacctcgagc
     1021 gacgaggtcg tcggcgtcac gctgcacgcc gtcggcgccc gcgcccgcaa cgtcacccgc
     1081 gcggaatccg aatcgccgtg cagcgcatgg gcgcccgccg tcaccggcag ggccgccgcc
     1141 agcagggcga ggacgaggga gtgaccggag cgtctcatag ctgcgcctgc acattcacgg
     1201 tgagaacgtc gtcgtaagac cccgccagaa ggcggctggg atcgacattg gccatggtca
     1261 ccgccagata gcccgactgg ttcatggccg ggacgttcga gcgcaacccg gcgcccgacg
     1321 ccatggcgca gcccgcgccg ccgccgtgca actgcgccga ggtgcaggtt tccgacaggg
     1381 cgctgccgtc gtcgcggcgc acttcgaaac gcgccgaata gctcgcctga tccgaaaatc
     1441 cggccgcgcg gctgcgcgcc gtcaggccgt ccgcgtccga ggccagcccg ccgtgcagcg
     1501 aactcagggt gaccgagaac ccggaattgc aatagacgcg gaacgggatg ttcaggctgg
     1561 cgcccgactg aagctccaca tcattgaccg gcgacggcgc gtcgagaaac ttgcattgct
     1621 cctgcacgcg cccggtcacc aggatatcga gcccctgttc aggcccgtcg aaaaccggca
     1681 gcgccccgga aggcgcattg tgatcccagg gccaatgcgt atgattgtgc gcatacgaga
     1741 cagtagagat caggcagccg agaatcagcg cggcggcgcc cggcaaaata cgacttttta
     1801 acggcaaccc atgtgggcga atcgtcgact tttgcatctg cgcccctccc tgttgcaatc
     1861 cgataagtca tcttaacgct ttgttaacgt ttgcatacga ggtagctccg agtcgatttg
     1921 gagtcaaacc tttttaatta agccgttttc aggcttactt ttcgggaatt tgcggattca
     1981 accaataaat gaatcggctt atacagatag atatcgcagt tattacttag taatagaaaa
     2041 aactatatgg gacgaagggc aattttttat cgaattaatt tttattttta ggaacagaat
     2101 ttatatgatt taaaaaaatc ttatgtattc gcaatcg
//
LOCUS       DASVLL010000572         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_470194,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000572 DASVLL010000000
VERSION     DASVLL010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470194"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF402_09405"
     CDS             <1..229
                     /locus_tag="VF402_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800554.1"
                     /translation="APMQILSFDLRPDTPQWNKAVEDNILILKADFPKLPAGPHTIHI
                     YRVDGNVVLERLVLDAGGLRPSYLGPPETAQ"
     gene            246..1454
                     /gene="manD"
                     /locus_tag="VF402_09410"
     CDS             246..1454
                     /gene="manD"
                     /locus_tag="VF402_09410"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEX7800555.1"
                     /translation="MKITKARVIVCSPGRNFVTLKIETDQGVYGIGDGTLNGRELAVV
                     SYLEDHVIPTLIGRDPRRIEDIWQYLYRGAYWRRGPVTMRAIAAVDVALWDIKAKMAG
                     MPLHDLLGGRSRDGVLVYGHANGADIGHTSEQVGHYRELGYKAIRAQSGIPGVKGAYG
                     VGRGDMYYEPADAALPTETLWNTASYMSFAPKLFERLRADHGDEVHLLHDVHHRLTPI
                     EAGRLGKDLEPYRLFWMEDATPAENQEAFRLIRQHTTTPLAVGEIFNTLWDAKDLISE
                     QLIDYLRMTIVGSGGVTHLRRVADFASVYQVRTGCHGATDLSPVTLGTAVHFDTWVPN
                     FGIQEYMRHAPETDEVFPHDYRFEDGYLHAGSAPGHGVDIDEALAAKYPYSPRQLPVA
                     RLQDGSMWNW"
     gene            1469..>1924
                     /locus_tag="VF402_09415"
     CDS             1469..>1924
                     /locus_tag="VF402_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HEX7800556.1"
                     /translation="MSEPVDHPLRATLSGLMHQRTIPVFASPAVVRSWVSLVGDEERA
                     AEAEWVATLEASEPASEDGRLAHTGAGGGMIWERHGEFSTWLKYDAAPGPRFAARKGL
                     GFCTLRTADFGWLCGAPGRIFLSVEIVVAGAEPKPAQFDGVIDLAQAVCC"
BASE COUNT          332 a          643 c          635 g          314 t
ORIGIN      
        1 tgcgcccatg cagatcctga gcttcgacct caggcccgat acgccgcaat ggaacaaggc
       61 ggtcgaggac aatatcctga tcctcaaggc cgattttccg aaattgccgg cgggaccgca
      121 cacgatccat atttaccgcg tcgacggcaa tgtcgtgctg gaacggctgg ttctggacgc
      181 cggcggcctc agaccgtcct atctcggccc gccggaaacg gcgcaatgat aacaagagaa
      241 gacccatgaa gatcaccaag gcccgcgtca tcgtctgttc gcccggacgc aacttcgtca
      301 cgctgaaaat cgagacggat cagggcgttt acggcatcgg cgacggcacg ctgaacgggc
      361 gcgaactggc cgtggtcagc tatcttgagg accacgtcat cccgaccctg atcgggcgcg
      421 atccgcgccg catcgaggac atctggcaat acctctatcg cggcgcctac tggcggcgcg
      481 ggccggtgac catgcgcgcc atcgcggcgg tcgacgtggc cttgtgggac atcaaggcca
      541 agatggccgg gatgccgctc cacgaccttc tgggcgggcg ttcgcgcgac ggggtgctgg
      601 tgtacggcca cgccaacggc gccgacatcg gccatacctc cgaacaggtc ggccattatc
      661 gcgaactggg ctacaaggcg atccgcgccc agtcgggcat tccgggcgtc aagggcgcct
      721 atggggtcgg gcgcggcgac atgtattacg aacccgccga cgcggccctg ccgaccgaga
      781 cgttgtggaa caccgcctca tatatgagtt tcgcgccgaa gctgttcgag cggctgcgcg
      841 ccgaccacgg cgacgaggtg catctgctgc acgacgtgca tcaccgcctg acgccgatcg
      901 aggccgggcg gctgggcaag gatctggagc cgtaccgcct gttctggatg gaggacgcca
      961 cgcccgccga aaatcaggag gccttccgcc tgatccgcca gcacaccacc acgccgctgg
     1021 ctgtgggcga gatcttcaac accctgtggg acgccaagga tctgatcagc gagcaactga
     1081 tcgactatct gcgcatgacc atcgtcggtt ccggcggcgt cacgcacctg cgccgcgtcg
     1141 cggacttcgc ctcggtgtat caggtgcgca ccggctgcca cggcgccacc gacctgtcgc
     1201 cggtcacgct ggggacggcg gtgcatttcg atacgtgggt tccgaacttc ggcattcagg
     1261 aatatatgcg ccacgcgccg gaaaccgacg aggttttccc gcacgactac cgcttcgagg
     1321 acggttacct gcacgccggt tccgcgccgg gccacggcgt cgacatcgac gaggcgctgg
     1381 cggcgaaata tccttatagc ccaagacagt tgccggtcgc gcgcctgcaa gacggcagca
     1441 tgtggaactg gtgatccggt aaaccgccat gtccgaaccc gtcgaccacc ctttgcgcgc
     1501 cacgctcagc ggcctgatgc accagcgcac catcccggtg ttcgcgtcgc ccgccgtggt
     1561 gcgaagctgg gtcagcctcg tcggcgacga ggagcgcgcc gccgaagccg aatgggtcgc
     1621 cacgctggag gcttccgaac cggcctcgga ggatgggcgg ctcgcgcaca ccggcgcggg
     1681 cggcggcatg atctgggagc gccacggcga gttttcgacc tggctgaaat acgatgcggc
     1741 gcccggtccg cgtttcgccg cgcgcaaggg gctgggcttc tgcaccctgc gcacggcgga
     1801 tttcggttgg ctgtgcggcg cgccggggcg gatcttcctc tcggtcgaga tcgtcgtcgc
     1861 cggcgccgag ccgaagcccg cgcagttcga cggcgtgatc gacctggcgc aggccgtgtg
     1921 ctgc
//
LOCUS       DASVLL010000573         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_199960,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000573 DASVLL010000000
VERSION     DASVLL010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199960"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1582)
                     /gene="lpdA"
                     /locus_tag="VF402_09420"
     CDS             complement(161..1582)
                     /gene="lpdA"
                     /locus_tag="VF402_09420"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7800557.1"
                     /translation="MADDLNFDVVIIGGGPGGYNAAIRAGQLGLKTAIVESRGVLGGT
                     CLNVGCMPSKALLHASELFEAAGHEFADIGIDVPAPKLNLGQMMKSKADSVTALTKGV
                     EFLMKKNKVTYFVGRGQIAGHDGSWGKVVVTGDDGATKNLTTRNIVIATGSEPTPLPG
                     VEVDQKQIVDSTGALSLSSVPKSLVVIGAGIIGLELGSVWRRLGAQVTVVEFLDRITP
                     GMDAEVAAAFKRTLEKQGVTFKLGTKVTAAKPGAKGVELTLEPAAGGAAEKLSADVVL
                     VAIGRRPYTQGLGLDSVGVTPDKRGFIPVDHFKTSAPGVWAIGDVILGPMLAHKAEED
                     AVACIELIAGKAGHVDYNLVPSVVYTRPEVAWVGQTEDQVKASGVAYKTGKFPFTANS
                     RAKINHETDGFVKFIADAKTDKVLGVHMMGPQVGEMIAEACVLMAFSGASEDLARICH
                     PHPTRSEAVRQASMGVENWTMQA"
     gene            complement(1694..>2210)
                     /locus_tag="VF402_09425"
     CDS             complement(1694..>2210)
                     /locus_tag="VF402_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX7800558.1"
                     /translation="MGFFAKAVVAALKDIPAVNAEIDGTDIIYKNHYDLGIAVGTEKG
                     LVVPVLRDVDTLSLAGIEKGISALGKQARDGSLSIDQLQGGTFTITNGGIYGSLMSTP
                     ILNMPQSGILGMHNIVQRAMVVNGQIVARPMMYLALSYDHRIVDGKEAVTFLVKVKEG
                     LEDPQRFILDV"
BASE COUNT          347 a          714 c          741 g          408 t
ORIGIN      
        1 ggaccgcgat ttcgaggcgg tgtacaggct ttgcgcgcag gcgtcgcaag ccttgctggc
       61 gaagctgttg cggtgaacgc ggcggcccgc ctgtctgccg acgcataaaa aaagagcgag
      121 ccgcgcggcc cgctcttccg ggttcggtct ttgccgcgag ttacgcctgc atcgtccagt
      181 tttcgacgcc catcgacgcc tgacggacgg cttcggagcg ggtgggatgc gggtggcaga
      241 tgcgggccag gtcttcggaa gcgccggaga aggccatcag cacacaggct tcggcgatca
      301 tttcgccgac ctgcggaccc atcatgtgca cgcccagcac cttgtctgtc ttggcgtcgg
      361 cgatgaactt cacgaagccg tcggtttcgt ggttgatctt ggcgcggctg ttggcggtga
      421 aggggaattt gccggtcttg tacgcaacgc ccgacgcctt gacctgatct tcggtctggc
      481 cgacccaggc gacttcgggg cgggtataga cgacgctggg gaccagattg taatcgacgt
      541 gaccggcctt gcccgcgatc agctcgatgc aggcgaccgc gtcctcctcg gccttgtgcg
      601 ccagcatcgg gcccaggatc acgtcgccga tggcccagac gcccggcgcc gaggtcttga
      661 aatgatcgac ggggatgaag ccgcgcttgt ccggcgtgac gccgacgctg tcgaggccca
      721 gaccctgcgt atagggacgg cggccgatgg cgaccagcac cacgtcggcg ctcagctttt
      781 cggcggcgcc gcccgcggcg ggctccagcg tcagctcgac gcccttggcg cccggcttgg
      841 cggcggtcac cttcgtgccc agcttgaagg tcacgccctg cttttccagc gtgcgcttga
      901 aggcggcggc gacttcggca tccatgcccg gcgtgatgcg gtcgaggaat tcgaccaccg
      961 tgacctgcgc gccgaggcgg cgccagaccg agccgagttc gaggccgata atgccggcgc
     1021 cgatcacgac cagcgacttg ggcacgctgg ataagctgag cgcgccggtg gaatcgacga
     1081 tctgcttctg atcgacctcg acgccgggca gcggggtcgg ctcggagccg gtggcgatga
     1141 cgatgttcct ggtggtcagg ttcttggtcg cgccgtcatc gccggtcacg acgaccttgc
     1201 cccaggaacc atcatgcccc gcgatttggc cgcgcccgac gaagtaggtc accttgttct
     1261 tcttcatcag gaactcgacg cccttggtca gggccgtgac gctgtccgcc ttggacttca
     1321 tcatctgacc gaggttcagc ttgggcgccg ggacgtcgat gccgatatcg gcgaactcat
     1381 gcccggcggc ctcgaacagc tccgaggcgt gcagcagcgc cttggacggc atgcagccga
     1441 cgttcaggca ggtgccgccc agcacgccgc gcgattcgac gatggcggtt ttcaggccca
     1501 gttggcccgc gcggatcgcc gcgttgtagc cgcccggccc gccgccgatg atgaccacgt
     1561 caaaattcag atcgtcagcc atgttctggc cctttccaac ttaaatcctt gccgcgcgcg
     1621 gagcgcaagc gcggcgcctg ccttgtctga aatcgccccg cggaccgggc cgcggggcgg
     1681 aacggcgtcc cgatcagacg tcgaggatga agcgttgcgg gtcttcgaga ccttccttga
     1741 ccttgacgag gaaggtcacc gcctccttgc cgtcgacgat gcggtgatcg taggacaggg
     1801 ccaggtacat catcgggcgg gccacgatct gaccgttcac caccatcgcg cgctgcacga
     1861 tgttgtgcat gcccaggatg cccgattgcg gcatgttgag gatcggggtc gacatcagcg
     1921 agccgtagat gccgccgttg gtgatggtga aggtgccgcc ctgcaactga tcgatcgaga
     1981 gcgagccgtc gcgcgcctgc ttgcccagag ccgagatgcc cttttcgatg cccgccagcg
     2041 acagcgtgtc gacgtcgcgc agcaccggca cgacgaggcc cttttcggtg ccgaccgcga
     2101 tgccgagatc gtagtggttc ttgtagatga tgtccgtgcc gtcgatctcg gcgttgaccg
     2161 ccgggatgtc cttcagcgcg gcgaccacgg ccttggcgaa gaagcccatg
//
LOCUS       DASVLL010000574         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_200022,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000574 DASVLL010000000
VERSION     DASVLL010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200022"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VF402_09430"
     CDS             <1..363
                     /locus_tag="VF402_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7800559.1"
                     /translation="GFPDRLGMEELRGLVREHRLIAMDDVDLAESNFIVLSRQPLPII
                     KLDRDFVDQLTRSDGTRLRQISAFVRLQNHYVVAEGVETPEQERLLKDAGVQYAQGWL
                     YSKSVSAEDFQAFYAAHA"
     gene            complement(670..2973)
                     /locus_tag="VF402_09435"
     CDS             complement(670..2973)
                     /locus_tag="VF402_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX7800560.1"
                     /translation="MADRKIPGIRPYHAPAGGWGALKATAKAVHEQMNAPEAAVTLLR
                     TNQPEGFDCPGCAWPDKEHRSTFRFCENGAKAVTWEATNKRVPPTFFAAHTVTSLRDW
                     SDYQLEDQGRLTHPMAYNAEKDIYEAVGWDDAFAHIGEILRQQATPDAVEFYTSGRAS
                     NEAAFLYQLFAREYGTNNFPDCSNMCHEATSVGLPQSIGIGKGTVSLDDFDHCELIIA
                     MGHNPGTNHPRMMGTLHEVARRGAPIIVFNPLRERALERFADPQDPLEMATLGATEIA
                     STYLQPRVGGDAAALKGIMKALLEMDEAQGPAAGVLDRAFIAAHTNGFDALAADLRAA
                     SWSGIEATSGLAEADLRQVAEAYAKSTATIVTYGMGLTQHRTGTQNVQQIANLLLLRG
                     NFGKPGAGICPLRGHSNVQGDRTVGITEKPGPAMLQRIEQRFGFRPPADHGHDAVAAM
                     QAIVEGRSKVLICLGGNLAVALPDPEACFPAMRQLDLSVHIATKLNRSHLLTGRHTFI
                     LPCLGRTERDLQAGGPQSVTVEDSMSMVHASRGKLAPASDALRSEPAIVAGMAAATLP
                     ASKVPWMHLIEDYDRIRDAIEAVFPDFASYNARIRVPGGFRLPLPPTERIWPTPSGKA
                     EFLVFDGLAEDPVSVDPAVLKLTTLRSHDQYNTTIYGLDDRYRGVFGRRDVLFMNEAD
                     LAERGLEEGDELLVETAFDHGPQRRLAGVVAVAYPIARGSVATYYPEGNCLVPLDYID
                     RRSGTPAYKSVPVRVTRIAGADAQAVA"
     gene            complement(3193..>3469)
                     /gene="ssrA"
                     /locus_tag="VF402_09440"
     tmRNA           complement(3193..>3469)
                     /gene="ssrA"
                     /locus_tag="VF402_09440"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          515 a         1102 c         1267 g          585 t
ORIGIN      
        1 ggcttcccgg accggctggg gatggaagag ctgcgggggc tggtcaggga gcaccgcctc
       61 atcgcgatgg acgatgtcga cctggccgag agcaacttca tcgtgttgtc gcgccagccc
      121 ttgcccatca tcaagctgga ccgggatttc gtcgaccagc tcacccgctc ggacgggacg
      181 cggttgcggc agatctcggc gttcgtgcgc ctgcagaacc actacgtcgt ggccgaaggg
      241 gtggagaccc ccgagcagga gcgtctgctg aaggacgcgg gcgtccagta cgcccagggg
      301 tggctgtatt cgaagtcggt gtccgccgag gatttccagg cgttctacgc cgcccatgcg
      361 tgacaggctt gccatccggc caggccggcg aagcgcctgc cgcggtcatc cgtgcgggtt
      421 cgaggtgtct cgcgcggcgg tgtcgtgcgc ggggactttc gtattccatc gaccggtgtc
      481 ttcggcatct caccccggcg gtggaatctt gccgcaccga ccggcctgcg cttccggcgg
      541 actcggcgcg agcggaaaag ggaacgccgc catgccgtca tccgagggaa ccgagggtcg
      601 cggcgtccgc gctgaacctg cggagcgacg cgatgccgcg gccccgcgag gcccttgcgg
      661 cccgcatgcc taggccacgg cctgggcgtc ggcgccggcg atccgcgtca cgcgcaccgg
      721 gacggatttg taggcgggag taccgctgcg ccggtcgatg tagtccaggg gaaccaggca
      781 gttgccctcg gggtagtagg tggcgaccga acccctggcg atcgggtagg ccacggcgac
      841 cacgccggcc aggcggcgtt gcgggccatg gtcgaacgcc gtctccacca gcagttcgtc
      901 gccttcctcg aggccgcgct cggccaggtc cgcctcgttc atgaaaagta cgtcgcgccg
      961 cccgaacacg ccgcggtaac gatcgtccag cccgtagatg gtggtgttgt actggtcgtg
     1021 gctgcgcagc gtggtcagct tgaggacggc ggggtccact gagacgggat cctcggcgag
     1081 gccgtcgaag accaggaatt cggccttgcc cgagggcgtg ggccagatgc gttcggtcgg
     1141 cggcagcggt agccggaagc cgcccgggac ccggatgcgc gcgttgtacg aggcgaaatc
     1201 ggggaacacc gcttcgatgg cgtcgcggat gcggtcgtag tcctcgatca ggtgcatcca
     1261 cggcaccttg ctggccggca gcgtggcggc cgccatgccg gcgacgatgg ccggctccga
     1321 tcgcaacgcg tcggaggccg gcgccagctt gccgcgcgag gcatgcacca tcgacatgga
     1381 atcctccacg gtgaccgatt gcggcccgcc ggcctgcagg tcgcgctcgg tccgtcccag
     1441 gcacggcagg atgaacgtgt gcctgcccgt gagcaggtgg gaacggttga gtttggtggc
     1501 gatgtgcacc gacaagtcga gctgccgcat ggccgggaag caggcctcgg ggtcgggcag
     1561 ggcgacggcc aagttgccgc ccaggcagat cagcaccttg gatcgtccct cgacgatggc
     1621 ctgcatggcg gcgacggcgt cgtggccgtg gtcggccggc ggccggaaac cgaaccgctg
     1681 ctcaatccgc tgcagcatgg ccggtccggg cttttcggtg atgccgacgg tgcggtcgcc
     1741 ctgcacgttg gagtggccgc gcagcgggca gatcccggct ccgggcttgc cgaagttgcc
     1801 gcgcagcagg agcaggttgg cgatctgctg gacgttctgc gtgccggtgc ggtgctgggt
     1861 cagccccata ccgtaggtga cgatggtcgc cgtggacttg gcgtaggcct cggcgacctg
     1921 gcgcaggtcg gcctcggcca ggccggaggt cgcctcgatg ccggaccacg acgcggcgcg
     1981 gagatcggcg gcgagcgcgt cgaagccgtt cgtgtgcgcc gcgatgaacg cgcggtcgag
     2041 cacgccggcc gcgggcccct gcgcctcgtc catctccagc agcgccttca tgatgccttt
     2101 cagggccgcc gcatcgccgc ccacccgcgg ctggaggtag gtcgaggcga tctcggtggc
     2161 gccgagggtc gccatttcca gcggatcctg cggatcggcg aagcgctcca gcgcgcgttc
     2221 gcgcaaaggg ttgaacacga tgatcggcgc cccgcgacgc gcgacctcgt gcagcgtgcc
     2281 catcatgcgc ggatggttcg tgccggggtt gtgacccatc gcgatgatca gttcgcagtg
     2341 gtcgaaatca tcgagcgaga cggtgccctt gccgatgccg atggactgcg gcaggcccac
     2401 gctcgtggcc tcgtggcaca tgttcgagca gtccgggaaa ttgttggtgc cgtattcgcg
     2461 ggcgaacaac tggtacagga acgcggcctc gttggaggcg cgcccggacg tgtagaactc
     2521 gaccgcgtcg ggggtcgcct gctgccgcag gatctcgccg atgtgggcga aggcgtcgtc
     2581 ccagccgacg gcttcgtaga tgtctttttc cgcgttgtag gccatcgggt gggtgagccg
     2641 cccctggtct tccagctggt agtcgctcca atcgcgcagc gaggtgacgg tatgggccgc
     2701 gaagaaggtg ggcgggacgc gcttgttggt ggcttcccag gtcaccgcct tggcgccgtt
     2761 ttcgcagaac cggaaggtgg accgatgctc tttgtcgggc caggcgcagc ccgggcagtc
     2821 gaacccttcc ggctggttgg tacgcaggag cgtcacggcg gcttccgggg cgttcatctg
     2881 ctcgtggacg gccttggccg tggccttgag cgcgccccag cccccggccg gtgcgtgata
     2941 gggacgaatg ccgggaatct ttctatctgc catgacaccc tcgccgagaa ggactggacg
     3001 cgtcgcgggg tttcccccgg aatgaggcgg caccctagtg tgcgcgaccc gggacgaagt
     3061 gcgccagtcc gtcgggcccg cggctcgatc cgggcggcgg ggcgttccga cgcgggatgc
     3121 agacttgtcc cgaagcccgc cttcgcaaaa agaaacggca ggtcacgtgg atgccgtgac
     3181 ctgccgctgg attggtggag gtggcgggag tcgaacccgc gtccgaaggc gttcaacgcc
     3241 cagcactaca tgcttagctc accgttggat ctcgtcccgc gacagcacga tgtgcgaagc
     3301 gcatcgaagg actagcctgc ttgatttgac cctggccgac aggcggcggc ttcgggcgat
     3361 cccgtgataa tgaccctaca ccgacgagca cgagcacaag tgggttcggg gctaggcctt
     3421 aggcggccag agcgtagttg tcgtcgttgg caactatgag tttgcggct
//
LOCUS       DASVLL010000575         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_92934, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000575 DASVLL010000000
VERSION     DASVLL010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92934"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF402_09445"
     CDS             <1..323
                     /locus_tag="VF402_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX7800561.1"
                     /translation="EMERRGVRFRSLTEAIDTATPTGRLILQIFGAVAEFERAVNRER
                     TRAGLAAARARGRTGGRPRALSSEKAKAAQAMLAEGLARAPDIAARFGVATSTLYRYL
                     PRKA"
     gene            complement(320..931)
                     /locus_tag="VF402_09450"
     CDS             complement(320..931)
                     /locus_tag="VF402_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7800562.1"
                     /translation="MSAASSASKPSPIASVCVYCGSAFGASPAYRQAARDLGQVLAGA
                     GLRLIYGGGGVGLMGETARAAHEAGGKVVGIMPQFLRGRELLLEDVETRMVANMHERK
                     MTMFEEADAFVVLPGGVGTLEEIVELLSWRRLDLHKKPVVFLNTNNYWAPFFALIEHT
                     LQEGFTPDSFRGVYVSVDAVADVLPAIADMRTHDMDIDTRKAV"
     gene            1064..1858
                     /locus_tag="VF402_09455"
     CDS             1064..1858
                     /locus_tag="VF402_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800563.1"
                     /translation="MNARLHIPALIALALALAPVLSGCHHPAAQTDDSDEAALRRDGY
                     FRAPQIVGVEQAGGGAYVVSGVATPNGRVRFAYGGDRAIGVTADAKGRFRAPLPAGPV
                     GGLYDVSTDDSGRLLHAEGRLFVPEGHPEKSVLIRAGAPSAPLLDKSAPIAVVDYDAA
                     GAIAVSGHIVTRDSIDVLIDGDIRAQVKPAADGAYSALSQIPPPTKTPVRVVLAAQAD
                     GKSATRTISVSLPDATPGDHMSLIDGGGWRVDATLPGGGVQTTLVF"
     gene            complement(1863..2348)
                     /locus_tag="VF402_09460"
     CDS             complement(1863..2348)
                     /locus_tag="VF402_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800564.1"
                     /translation="MLKTFAVLLLVTTGMLTFASAKAAVSCQAVADEVSRKGADATVR
                     DLTSRTNGDWDQFIRQVGAGRAECVQAAAAVAPGADAGNHEGIIYGLSQALRENPKAV
                     LMVSGGRISLENVCSDRAIEDSDAQHKAFMQRAAASVAQIREPSLRVKRDACLTALHR
                     Q"
BASE COUNT          431 a          801 c          847 g          394 t
ORIGIN      
        1 aggagatgga gcggcgtggc gtacgcttcc gctcgctgac cgaagccatc gacacggcga
       61 cgccgacggg caggctgatc ctgcaaatct tcggcgcggt ggcggagttc gagcgcgccg
      121 tcaaccgcga acgcacccgg gccggtctgg cggcggcgcg ggcgcgcgga cggaccggcg
      181 gacggccgag ggcgctgtcg agcgaaaagg ccaaggcggc tcaggccatg ttggccgaag
      241 ggctggcccg cgcgccggac atcgccgcgc gtttcggcgt ggcgacctca accctctaca
      301 ggtacttgcc gcgcaaggct tagaccgcct tgcgcgtgtc gatgtccatg tcgtgcgtgc
      361 gcatgtcggc gatggcgggc agcacgtcgg cgaccgcgtc gacgctgaca tagacgccgc
      421 ggaagctgtc cggggtgaag ccttcttgca gcgtatgttc gatcagcgcg aagaacggcg
      481 cccagtaatt attggtgttg agaaagacca ccggcttctt gtgcaggtcg aggcggcgcc
      541 aggacagcag ttcgacgatc tcctccagcg tgccgacgcc gccgggcaga acgacgaagg
      601 cgtcggcttc ctcgaacatc gtcatcttgc gttcgtgcat gttggcgacc atgcgcgtct
      661 cgacgtcctc aagcagcagt tcgcgcccgc gcaggaattg cggcatgatc cccaccacct
      721 tgccgcccgc ctcgtgcgcg gcgcgcgcgg tttcgcccat caggcccacg ccgccgccgc
      781 cgtagatcag gcgcaggccc gcgcccgcca gcacctgccc cagatcgcgc gcggcctgac
      841 gataggcggg cgaggcgccg aaggcggagc cgcagtaaac gcagacggag gcgatgggcg
      901 agggcttgga ggcggaagac gcggcagaca ttcggttttg cagtcctgta tgtagataaa
      961 ggatgggggc gaaagctacg cccgatggcg cgcaaggaaa caataggttt gcaacaatct
     1021 aggctactag cggcgcaaaa gaaaagcgga tagtgaagtt ggcatgaacg cgcgtttgca
     1081 tattccggcg ctgatcgcgc tggccctggc cttggctcct gttctgagcg gctgccatca
     1141 tccggcggcg cagaccgacg acagcgacga ggcggccctg cgccgcgacg gctatttccg
     1201 cgcgccgcag atcgtcgggg tcgagcaggc cggcggcggc gcctatgtcg tcagcggcgt
     1261 cgccacgccc aacgggcgcg tgcgcttcgc ttacggcggc gaccgcgcga tcggcgtgac
     1321 ggcggacgcc aaggggcggt tccgcgcgcc gctgcccgcc gggccggtgg gagggcttta
     1381 cgacgtgtcg accgacgaca gcggacgcct gctgcacgcc gaagggcggc tgttcgtccc
     1441 ggaggggcat cccgaaaagt cggtgctgat ccgcgccggg gcgcccagcg cgcctttgct
     1501 ggacaagagc gcgccgatcg ccgtggtgga ttacgacgcc gccggagcca tcgccgtcag
     1561 cggccatatc gtgacgcgcg acagtatcga cgtgctgatc gacggcgata tacgcgcgca
     1621 ggtgaagccc gccgccgacg gcgcctacag cgccctgtcg cagatcccgc cgccgacgaa
     1681 aacgcccgtg cgcgtcgtgc tggcggcgca ggcggacggc aagtcggcga cgcgcacgat
     1741 cagcgtctcg ctgcccgacg ccacgccggg cgatcatatg agcctgatcg acggcggcgg
     1801 ctggcgcgtc gacgctaccc tgccgggcgg cggggtgcag acgaccctgg ttttttaaaa
     1861 agtcattgac gatgcaaggc agtcaggcag gcgtcgcgct ttacccgtag cgacggctcg
     1921 cggatttgcg ccaccgaggc ggcggcgcgt tgcataaacg ccttgtgctg cgcgtcggag
     1981 tcttcgatag cgcgatcgct gcatacgttt tccagcgaaa tacgaccgcc cgacaccata
     2041 agaacggctt tgggattttc ccgcagggcc tggctaagcc cgtagatgat cccctcatgg
     2101 ttgccggcgt cggcgccagg cgcgacagcc gctgcggcct ggacgcactc cgcgcggccg
     2161 gcgccgacct gacggatgaa ctgatcccag tcgccgttgg tgcggctcgt caggtcacgg
     2221 acagtcgcat ccgcgccttt tcggctcact tcgtccgcaa ccgcctgaca ggaaacagcc
     2281 gccttcgcgg acgcaaacgt cagcatgccc gtcgttacaa gcaaaagcac ggcaaaggtt
     2341 ttcaacataa gtcctctcct caacaggatg cggataaatc gaagtcgcgg atgccgaggc
     2401 tataaataaa ggtacgtgac gcttttcgga gtaagggcca gggcccttac atcatctcgt
     2461 cctgtttctg ctt
//
LOCUS       DASVLL010000576         5423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_308430,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000576 DASVLL010000000
VERSION     DASVLL010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308430"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VF402_09465"
     CDS             complement(<1..473)
                     /locus_tag="VF402_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800565.1"
                     /translation="MSGLGAAALAGCAGRAVAAGSSPAAPPDPAQALTSLNGEWRFAL
                     GKTPADIDGLANFHQPDVDLGAFRATPVPSNWALQGYEKPQYAYRKGDFASEGFYLRH
                     FDAPADWSERRVLLHFGGVWDSAEVWLNGQPLGRQDTGFNKISWDVTPQIKPGAA"
     gene            complement(771..1505)
                     /locus_tag="VF402_09470"
     CDS             complement(771..1505)
                     /locus_tag="VF402_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800566.1"
                     /translation="MARATKKTTSGGEKTPATDIPADAGVKKTLARKAPAPKATAKAV
                     KDAEKKVAAAVKKTAAASKTLVKDVEKPVVKAAAKAEKAVKTTAAKAEKTVKKTAATA
                     RAKVASATKTAEKKVEKAVAQAKPAARKATQQTAAKLEAVSEAAKASIAEAGETAARK
                     IEEMRAAAEPALKDIQKKAEAAVASAQEKIEDAVEQAKPHVEDFARKADAFTADVAAK
                     AHEATGKAAEVTQKKPGFWARLFGAR"
     gene            1617..2483
                     /locus_tag="VF402_09475"
     CDS             1617..2483
                     /locus_tag="VF402_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7800567.1"
                     /translation="MTYNPQQIVARGPRRVAEAAAEAIDADALLEGATYSILEEDEDH
                     DVWRIDAFPTAEEEDARLQGLLGGYPELKLTVEALADADWLAMALSGLPPVRAGRFFI
                     YGIHDRGQVPASTVNLRIEAGAAFGTGHHGTTVGCLIAYNDLLKARRFNRVLDVGAGT
                     GVLAIAASKTGSKTAVGTDIDPISVRISNENAALNQSRARFVHANGLNHRKVRRDAPY
                     DLVFANILARPLVSLSMPIRGALKPGGLVILSGLLRTQERFVKAAYLSHGFRLVRRIH
                     RDAWCTLVMQKA"
     gene            2563..3084
                     /locus_tag="VF402_09480"
     CDS             2563..3084
                     /locus_tag="VF402_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800568.1"
                     /translation="MSLSRRALMASALALTGAGFAARAVAQTAPQTVPMPVYDEALVN
                     NWQNWSWAKVELSVEAGGAKPLRAEGTPYQAVAFHHDPFDAAPYSKLTFFANGGADGG
                     QSLMVKALVGGKAVDPGVTITLKARTWQPAAVPLKDLLGPGSVAGKMIDGLWFQAGAD
                     GAKPFYLDKIQFE"
     gene            3159..4988
                     /locus_tag="VF402_09485"
     CDS             3159..4988
                     /locus_tag="VF402_09485"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX7800569.1"
                     /translation="MTADTAFFQSFDVTTSPAQGAGHVAALRAQMKAQGLDGFIVPHE
                     DEHQNEYLPDANERLAWVSGFTGSAGAAIIFMDRAVLFADGRYTLQSREQTDPAVFAV
                     EDFHTGSLSDAVASARPGAVIGYDARLLSPEHVGRLEAAARSAGVTLKAVSPNPLDLA
                     WGADRPAQPMAKIVPQPLEYAGRASADKRAEIAQALKNHGIAAALITAPSSLAWLFNI
                     RGGDVIRSPLPLGQTLLKNDGSAELFIEPAKISDGLLAWLGNTVAVKAPGEVEASLAA
                     FRGQSVLIDGQLSSAHWMQAIRDAGAAPLAADDPCLLPRACKNPVEIAGTRAAHIRDG
                     ALLTTFLHWVATEAQGAPFSEIEIAQKLESIRRASDGLKDLSFDTISGFGPHGALPHY
                     KVNVQSDMRIGKGNLLLVDSGAQFMDGTTDVTRTVAIGEPGDEHRRMFTLVLKGHIAL
                     ATVKFPAGTTGTQLDALARQFLWAGGFDYDHGTGHGVGVYLGVHEGPQRISKALNRYA
                     LQTGMIVSNEPGYYKTGAYGIRIENLQYVTEARVPEGGERPMHGFENLTWAPIDRRLI
                     EVAMLSDAERAYMNAYHAKVLELVGPRVSPDVRAWLQEVCAPL"
     gene            complement(4989..>5423)
                     /locus_tag="VF402_09490"
     CDS             complement(4989..>5423)
                     /locus_tag="VF402_09490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7800570.1"
                     /translation="DLIDTNVTGMLLCAREAFRVMKAQAPQGGRIINNGSVSAYAPRP
                     NAAPYAISKHAITGLTKALILDGRAHGILCSQIDIGNVVSDMSARMETGVSQADGSTR
                     PEPRMAMEHVVGTVLHMAALPPEANIPFVTVMASGMPLYGRG"
BASE COUNT          858 a         1926 c         1725 g          914 t
ORIGIN      
        1 ttggccgcgc cgggcttgat ctgcggcgtg acgtcccatg agattttgtt gaagccggta
       61 tcctgacggc ccagcggctg gccattcagc cagacctcgg cggagtccca cacgccgccg
      121 aagtgcagca gcacgcgccg ctcgctccag tccgcagggg cgtcgaaatg gcggagatag
      181 aagccctcgc tggcgaaatc gcctttccgg taagcgtatt gcggcttctc atagccttgc
      241 agcgcccagt tggacggcac gggcgtcgcc cggaacgcgc cgaggtcgac atccggctga
      301 tggaagttgg ccagcccgtc gatatcggcg ggggtcttgc ccagggcgaa gcgccattcc
      361 ccgttcagcg aggtcagggc ctgcgccgga tcgggcggcg cggcaggcga cgatccggcg
      421 gcgaccgccc gtcccgcgca tccggccagc gccgccgcgc cgagcccgct catcagatcc
      481 cggcgggagg gcctccacgc agcccggttc ttgtccgtca tcctgtgcgc cttccctgcc
      541 aaaaacgtcc gccatgtccc gcctcacgcg gaggcggagc aaaaggagcg attcacgttg
      601 aaatcgtcag gcttcaacgg cgctcggttt tgatcatata agaccgatca tgatcaatat
      661 ggcaggtctt gacaccggta tcaagctgca tttttcgtcc ttcccggcca gcaaaaagcc
      721 cctccggcgc gggccggagg ggctgatctt gatccgcgta acgggacggc ttatcgcgcg
      781 ccgaacagac gcgcccagaa gccgggcttc ttctgcgtga cctcggcggc cttgccggtc
      841 gcctcgtgcg ccttggcggc gacgtcggcg gtgaaggcgt cggccttgcg ggcgaagtcc
      901 tcgacatgcg gcttggcctg ttcgacggcg tcctcgatct tctcctgcgc ggaggcgacg
      961 gcggcttcgg ccttcttctg aatgtccttg agcgccggtt cggcggcggc gcgcatttcc
     1021 tcgatcttcc gggcggcggt ttcaccggcc tcggcgatcg acgccttggc ggcctcgctg
     1081 acggcctcca gcttggcggc ggtctgctgc gtggccttgc gcgcggccgg cttggcctgg
     1141 gcgacggcct tttcgacctt cttctcggcg gtcttggtcg cggaggccac cttggccctg
     1201 gcggtcgcgg cggtcttctt caccgtcttc tccgccttgg ccgccgtcgt cttgacggcc
     1261 ttttccgcct tggccgcggc cttcaccacc ggcttctcga catccttgac cagagtcttc
     1321 gacgccgccg ccgtcttttt gaccgccgcg gcgaccttct tctcggcgtc cttcaccgct
     1381 ttggcggtcg ctttgggcgc gggcgccttg cgcgccagcg tctttttcac gccggcgtcg
     1441 gcaggaatat ccgtcgcggg cgttttctcg ccgcccgaag tggttttttt ggtagctctc
     1501 gccatcgtct gtccttaaat gcctgtcgct taacaaaccg catcgtgcag attcgacgtt
     1561 atccgaatcg gagcggggaa tcaacgaaac cgttacagag tttaccgaag ggcgccatga
     1621 cctacaatcc gcaacagatc gtcgcgcgcg gcccgcgccg cgtcgccgaa gccgccgccg
     1681 aggccatcga cgccgacgcc ctgctggaag gcgccaccta ttcgatcctt gaggaggacg
     1741 aggaccacga cgtctggcgc atcgacgcgt ttcccaccgc cgaagaagag gacgcgcggc
     1801 tgcaaggcct gctgggcggc tatcccgaac tcaagctcac ggtcgaggcc ctggccgacg
     1861 ccgactggct ggccatggcg ctgtccggcc tgccgccggt gcgcgcgggc cgcttcttca
     1921 tctacggcat ccacgatcgc ggtcaggttc cggccagcac ggtcaacctg cgcatcgagg
     1981 cgggcgccgc cttcggcacc ggccaccacg gcaccacggt cggctgcctg atcgcctata
     2041 acgacctcct gaaagcgcgc cggttcaacc gcgtgctgga cgtcggcgcc ggcacgggcg
     2101 tgctggccat cgccgcctct aagacgggat cgaaaaccgc cgtcggcacc gacatcgacc
     2161 cgatctcggt gcgcatctcc aacgagaacg ccgcgctgaa ccagagccgc gcgcgcttcg
     2221 tccacgccaa cggcctcaac caccgcaagg tccgccggga cgcgccctac gatctggtct
     2281 tcgccaacat cctggcgcgc cctctggtca gcctttccat gccgatccgc ggcgcgctga
     2341 aacccggcgg cctcgtcatc ctgtcgggcc tcttacgcac gcaggaacgg ttcgtgaagg
     2401 ccgcctatct gagccacggc ttccggctgg tccggcgcat ccaccgtgac gcgtggtgca
     2461 cactggtgat gcaaaaagcc taggcggcgc ggcgccctct tgcacttgta cgataattgc
     2521 gcattaaatc tttcaaatta caaaattcag ggagatttaa tcatgtcgct ttcacgccgc
     2581 gccctcatgg cgtccgcgct ggccttgacg ggcgccggtt tcgccgcccg cgccgtcgcg
     2641 caaaccgccc cgcagaccgt ccccatgccg gtctatgacg aggcgctggt caacaactgg
     2701 cagaactgga gctgggccaa ggtcgagctg agcgtcgagg cgggcggggc aaaacccttg
     2761 cgcgccgagg gaactccata tcaggccgtc gccttccacc acgatccgtt cgacgccgcg
     2821 ccctacagca agctgacctt cttcgccaac ggcggcgccg acggcggcca gagcctgatg
     2881 gtcaaggcgc tggtcggcgg caaggcggtc gatccgggcg tcacgatcac cctgaaagcc
     2941 aggacatggc agcccgccgc cgttccgctc aaagacctgc tcggccccgg aagcgtcgcc
     3001 ggcaagatga tcgacggcct gtggttccag gccggcgccg acggcgccaa gccgttctat
     3061 ctcgacaaga tccagttcga gtaagattca tttgaaacgt ctaaacgcgg gtctatataa
     3121 gatcgcatac cgcgacattc cgctacgagg cccgcgccat gaccgccgac accgctttct
     3181 tccagtcctt cgacgtgacc accagcccgg cccagggcgc cggtcatgtc gcggcgctgc
     3241 gggcgcagat gaaggcgcag gggctggacg gcttcatcgt gccgcacgag gacgagcatc
     3301 agaacgaata cctgcccgac gccaatgagc ggctggcctg ggtgtcgggc ttcaccggct
     3361 cggcgggcgc ggcgatcatc ttcatggacc gcgccgtgct gttcgccgac gggcgctaca
     3421 cgctgcaatc gcgcgagcag accgaccccg ctgtcttcgc cgtcgaggat ttccacacgg
     3481 gctcgttaag cgacgcggtc gcctcggcgc gccccggcgc ggtgatcggc tacgacgcgc
     3541 gcctcctcag cccggagcat gtcggccgcc tcgaagccgc cgcccggtcg gcgggcgtga
     3601 cgctgaaagc cgtctcgccc aacccgctcg acctcgcgtg gggcgccgac cgcccggcgc
     3661 agccgatggc gaagatcgtc ccccagccgc tggaatacgc cggcagggcc tcggccgaca
     3721 agcgcgccga gatcgcgcag gcgctgaaaa accacggcat cgccgccgcc ctgatcaccg
     3781 cgccgtcgtc gctggcgtgg ctgttcaaca ttcgcggcgg cgacgtgatc cgctcgccgc
     3841 tgccgctggg ccagacgctc ctgaaaaacg acggctcggc ggagctgttc atcgaaccgg
     3901 ccaagatcag cgacggcctg ctggcgtggc tgggcaatac ggtcgcggtc aaggcccccg
     3961 gcgaggtcga ggccagcctc gccgccttca ggggtcagtc ggtgctgatc gacgggcaac
     4021 tgtcctcggc ccactggatg caggccatcc gcgacgcggg cgcggccccg cttgccgccg
     4081 acgatccgtg ccttctgccg cgcgcctgca agaacccggt cgagatcgcc ggaacccgcg
     4141 ccgcccacat ccgcgacggc gcgcttctga ccaccttcct gcactgggtc gccaccgagg
     4201 cgcaaggcgc gccgttcagc gagatcgaga tcgcgcagaa gctggaatcg atccgccgcg
     4261 cctcggacgg cctgaaagac ctgtcgttcg acaccatttc cggcttcggc ccgcacggcg
     4321 ccctgccgca ctacaaggtc aacgtccagt cggacatgcg catcggcaag ggcaatctgc
     4381 tgctggtcga ttccggcgcg cagttcatgg acggcaccac cgacgtgacg cgcaccgtgg
     4441 ccatcggcga accgggcgac gaacaccggc gcatgttcac cctggtgctg aaaggccata
     4501 tcgcgctggc gacggtgaag ttcccggcgg gcacaaccgg cacgcagctc gacgcgctgg
     4561 cgcggcagtt cctctgggcc gggggcttcg actacgatca cggcaccggc cacggcgtcg
     4621 gcgtctatct cggcgtccac gaaggcccgc agcgcatctc caaggcgctc aaccgctacg
     4681 cgctgcaaac cggcatgatc gtctcgaacg aacccggcta ctacaagacg ggcgcctacg
     4741 gcatccgcat cgagaacctg caatacgtca ccgaggcgcg cgttcccgaa ggcggcgagc
     4801 gcccgatgca cggcttcgag aacctgacct gggcgcccat cgaccgcagg ctgatcgagg
     4861 tcgcgatgct cagcgacgcc gagcgcgcct atatgaacgc ctaccacgcc aaggtgctgg
     4921 aactggtcgg tccgcgcgtg tcgcccgatg tgcgcgcctg gctgcaagag gtctgcgcgc
     4981 cgctgtaact agccccgccc gtacagcggc atccccgacg ccattacggt gacgaagggg
     5041 atattggcct ccggcggcag ggccgccatg tgcagcaccg tcccgacgac atgttccatc
     5101 gccatgcgcg gctccgggcg cgtcgatccg tccgcctgcg acacgcccgt ctccatgcgc
     5161 gccgacatgt ccgagacgac attgccgatg tcgatctgcg aacacagtat gccgtgcgcg
     5221 cgcccgtcga ggatcagcgc cttggtcagg ccggtgatgg cgtgcttgct tatcgcatag
     5281 ggcgcggcgt tcgggcgcgg cgcgtaagcc gacaccgaac cgttgttgat gatgcgcccg
     5341 ccttgcggag cctgcgcctt catcacgcgg aacgcctcgc gcgcgcacag caacatgccg
     5401 gtgacattgg tgtcgatcag gtc
//
LOCUS       DASVLL010000577         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_254191,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000577 DASVLL010000000
VERSION     DASVLL010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254191"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="argH"
                     /locus_tag="VF402_09495"
     CDS             <1..849
                     /gene="argH"
                     /locus_tag="VF402_09495"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7800571.1"
                     /translation="GRDRSRFADARVRMNENPLGSAALAGSPFPIDRFATSEALGFER
                     PMRNSLDGVSARDFALETLSDASICAGHLSRLAEEIVIWTTPYFGFVRLSDSFSTGSS
                     IMPQKRNPDAAELVRAKTGRINGALIALTTVMKGLPLAYSKDMQEDKPPVFEAFDALD
                     LALRAMTGMVRDLAANTDRMRAAAGAGFSTATDLADWLVRNLNIPFRDAHHITGAAVK
                     LAEQKGVDLAQLPLSDLQNLEARITDDVYKVLTPEASAASRQSYGGAAPDQVRLQIQR
                     WKDQRK"
     gene            846..1169
                     /locus_tag="VF402_09500"
     CDS             846..1169
                     /locus_tag="VF402_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800572.1"
                     /translation="MMNRNKMLAAAALTGAILAASLALSACGKVGDLKQAPPMFNHKA
                     ELSWSTSQNSGGGTTTTTDDSRSADTEKAKPDASDINHMPDPYTGNKKISDAPLEGFG
                     NAERH"
     gene            1190..>2408
                     /gene="lysA"
                     /locus_tag="VF402_09505"
     CDS             1190..>2408
                     /gene="lysA"
                     /locus_tag="VF402_09505"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7800573.1"
                     /translation="MNHFDYRDGELYAEGVALRELADRVGTPFYVYSTATLERHFNVF
                     SQAVSAPPELKSPTGEAPLVAFACKANSNMAVLTTLAQVGAGADTVSEGEIRKALAAG
                     IPASKIVFSGVGKTDEEMAFALRAGIYQLNVESRPELERLARVAASINCVAPFVIRIN
                     PGVGAGGHAKITTGGAKDKFGVSAAEALALYADSAAHPNLAPVGLACHIGSQITDLAP
                     MQAAFAKMRGWVMDLRAQGLPVERVDLGGGLGVPYFNQPEPPSPQAFAEMVALTMGDL
                     GVRYAFEPGRLIAANAGVLVSRVIHVHERQDSGQKFLVIDAAMNDLIRPALYDAYHDI
                     RPVAKAAGNASATYDVVGPICETGDTFTRGRELSEQAAGDLIAFMSAGAYGAVMGSEY
                     NSRPLAPEVLVRDE"
BASE COUNT          408 a          811 c          811 g          378 t
ORIGIN      
        1 ggacgcgacc gctcgcgttt tgccgacgcg cgcgtccgca tgaacgaaaa cccgctcggc
       61 tcggcggcgc tggccggatc gccgtttccg atcgaccgct tcgccacgtc ggaggcgctg
      121 ggcttcgagc ggccgatgcg caattcgctg gacggcgtgt cggcgcgcga tttcgcgctg
      181 gagaccttga gcgacgcgtc catctgcgcc ggacacttaa gccgtctggc cgaggagatc
      241 gtcatctgga ccacgcccta tttcggcttt gtgcgcctgt ccgattcctt ctcgaccggc
      301 tcgtcgatca tgccgcagaa gcgcaatccc gacgccgccg agctggtgcg cgccaagacg
      361 ggccgcatca acggcgcgct gatcgccctg accacggtga tgaagggcct gccgctggcc
      421 tattccaagg acatgcagga ggacaagccg ccggtgttcg aagccttcga cgcgctcgac
      481 ctggccttgc gcgccatgac cggcatggtg cgcgacctcg ccgccaacac cgacaggatg
      541 cgcgcggcgg cgggggcggg cttctcgacc gcgaccgacc tcgccgactg gctggtgcgc
      601 aacctgaaca tcccgttccg cgacgcgcac cacatcaccg gcgcggcggt caagctggcc
      661 gagcaaaagg gcgtcgatct ggcgcaactg ccgctgtccg acctgcaaaa tctggaagcc
      721 aggattacgg atgatgtgta taaggtgctg acccctgagg cctcggcggc ttcgcgccaa
      781 tcctatggcg gcgcggcgcc cgatcaggtt cgcttgcaaa tccagcgctg gaaggaccaa
      841 cgcaaatgat gaaccgcaac aagatgctgg ccgccgcggc tctgaccggc gccatcctcg
      901 ccgcctcgct cgccttgagc gcctgcggca aggtcggcga cctgaagcag gcgccgccga
      961 tgttcaacca taaggccgag ctgtcgtggt cgacctcgca gaacagcggc ggcggcacga
     1021 cgacgacgac cgacgattcg cgctcggcgg acaccgaaaa ggccaagccg gacgccagcg
     1081 acatcaacca catgcccgac ccctatacgg gcaacaagaa gatttccgac gccccgctcg
     1141 aaggcttcgg caacgccgaa cggcattagg aacggcgccg ggaccgaaga tgaaccattt
     1201 tgattaccgc gacggcgaac tgtacgccga gggcgttgcc ttgcgtgaac tggccgaccg
     1261 ggtcggcacg ccgttctacg tctattcgac cgcgacgctg gagcgccatt tcaacgtctt
     1321 ctcgcaggcc gtctcggccc cgccggagct gaaaagcccg accggcgaag cgccgctggt
     1381 ggcgttcgcg tgcaaggcca attccaacat ggcggtgctg acgacgctgg cgcaggtcgg
     1441 ggcgggcgcg gatacggtgt cggaaggcga gatccgcaag gcgctggccg ccgggattcc
     1501 ggcgtcgaag atcgtctttt ccggcgtcgg caagaccgat gaggaaatgg ccttcgcgct
     1561 cagggcgggc atctatcagc tcaatgtcga gtcgcgcccg gagcttgagc gcctcgcgcg
     1621 cgtcgcggcg tcgatcaact gcgtcgcgcc gttcgtgatc cgcatcaatc cgggcgtcgg
     1681 cgcgggcggc cacgccaaga tcaccaccgg cggcgccaag gacaagttcg gcgtctccgc
     1741 cgccgaggcg ctggccctct acgccgattc cgccgcgcac ccgaacctgg cgccggtggg
     1801 gctggcctgc catatcggca gccagatcac cgatctggcc ccgatgcagg cggcgtttgc
     1861 aaagatgcgc ggctgggtca tggacctgcg cgcgcagggc ttgccggtcg agcgcgtgga
     1921 tttgggcggc ggtctgggcg tgccctattt caaccagccc gaaccgccgt cgccgcaggc
     1981 cttcgccgaa atggtcgcgc tgaccatggg cgatcttggc gtgcgctatg cgttcgagcc
     2041 ggggcggctg atcgcggcca acgccggggt gctggtgtcg cgcgtcatcc acgtgcatga
     2101 gcgtcaggat tcggggcaga aattcctcgt catcgatgcg gcgatgaacg acctgatccg
     2161 tccggccctc tacgacgcct atcacgacat ccgcccggtg gcgaaagcgg cgggaaacgc
     2221 cagcgcgacc tatgacgtgg tcgggccgat ctgcgagacg ggcgacacct tcacgcgcgg
     2281 gcgcgagttg tccgaacagg cggcgggcga cctgatcgcc ttcatgtcgg cgggcgccta
     2341 tggcgcggtc atgggcagcg aatacaacag ccgcccgctc gcgccggagg tgttggtgcg
     2401 cgacgaag
//
LOCUS       DASVLL010000578         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_361344,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000578 DASVLL010000000
VERSION     DASVLL010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361344"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF402_09510"
     CDS             complement(<1..767)
                     /locus_tag="VF402_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800574.1"
                     /translation="MSYAVSRRSLFGGISILSLGALPLTARAQTPAAAPGAPPAVEVF
                     AAPAFVDQVALSPDGKTVALVTQRGDDKLLFYMNLADRKPQFQNMGKEKIRGLAWGDN
                     THILVISSSTMTLPLQFDKQELFFAHITNIVTGKVVNPADKLRKGFYNTIAAPRRVRI
                     GDSYRLYAASFGDVQGSCLFSYDLDGGYPELVATAPDLASGFLVAPDGYVLACEKFDS
                     LHKKWTLYANQNPPGKRPDFRAVYVNTKDISTIYTPG"
     gene            complement(860..>2900)
                     /locus_tag="VF402_09515"
     CDS             complement(860..>2900)
                     /locus_tag="VF402_09515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7800575.1"
                     /translation="LASTQQQGACIMTSKTSRRAFLATGAVAFAVGARFAGAQAAPAA
                     SGPPAIEIYAGSPLVDQIALSPDGQRIAIVSQKGDEKILLHYEIANPQPKSLSIGPAK
                     VRDLFWGDNAHVILVDSQTTALTGFAGYRHEFTLARCIDVDTGAVTTFFSNMEGFSNV
                     VLGGLQRVKTPAGYRVTAANYRLHTDDGTVSVYDGKFYLYSFSMDKAPPRRICEASQE
                     ATDFIVTAEGAPLAYVEHIPLHNEWRLYINATPTEKDPHFQFAYGVKDPVSLPIAQGV
                     GRDGLSVVVYFDKGDYEGQFREISADGKVSDPLDPQATLDRDALFHPVTRRLAGFRYY
                     DDWQRDDYFEPLLKKLVEALPQVVDPGDRVRIADFAEDPRKMIVYVENAHNAGAYVYL
                     DFTTGGGSPVASNYAALPPAWITQKQPIDYKAADGLPIHAYLTLPPDAALNGRPAKNL
                     PLIVLPHGGPWVRDHVDFDWQTQVLASRGYAVLQPNYRGSGGAGIAFMDAGNGEMGRK
                     MQTDLSDGVRYLVAQGLADPKRVAILGASYGGYAALAGATLDPGVYRCAVSIAGPSDV
                     GAIISYLLESTNNMDTPAVVAWRRVLGDRSGWDAISPAKQAAKAYCPILLIHGTDDTV
                     VPISQSQRMESALKAAGKPVEFVTYKGQDHWETIGSARIDMMKAALAFLQKYNPA"
BASE COUNT          487 a          892 c         1005 g          516 t
ORIGIN      
        1 atgccgggcg tgtagatggt cgaaatgtcc ttggtgttga catagacggc gcggaagtcc
       61 ggcctcttgc ccggcggatt ctgattggcg tacagcgtcc atttcttgtg cagggagtcg
      121 aatttttcgc aggcgaggac atagccgtcg ggcgcgacca gaaaaccgct ggccagatcg
      181 ggcgccgtcg ccaccagttc gggatagccg ccgtccagat cgtagctgaa caggcacgag
      241 ccctgcacgt cgccgaagct ggcggcgtag agccggtagg aatcgccgat cctgacgcgg
      301 cgcggcgcgg ctatggtgtt gtagaagccc ttgcgcagct tgtccgccgg gttcaccacc
      361 ttgcccgtga cgatgttggt gatgtgcgcg aagaacagct cctgcttgtc gaactgcaac
      421 ggcagggtca tggtgctgga ggaaatcacg aggatgtgcg tgttgtcgcc ccaggcgagg
      481 ccgcgaatct tctccttgcc catgttctgg aactgcggct tgcggtcggc gaggttcatg
      541 tagaacagaa gcttgtcgtc gccgcgctgc gtgaccaatg cgacggtctt gccgtcgggc
      601 gacagggcga cctgatcgac gaaggcgggg gcggcgaaga cctcgacggc gggcggcgcg
      661 ccgggcgcgg cggcgggcgt ctgggcgcgg gccgtcaacg gcagggcgcc gaggctcagg
      721 atcgagatgc cgccgaacag cgagcggcgc gaaacagcgt atgacatgga gccccttcca
      781 tagatcgagt tgcgctacgc taacgcggat gcccggacgt gacaagagtg gtttccaggg
      841 atacttcctc gcaggggtgt caggcggggt tgtatttttg caggaaggcc agcgcggcct
      901 tcatcatgtc gatgcgcgcc gagccgatgg tctcccagtg gtcctgcccc ttgtaggtga
      961 cgaactcgac cggcttgccc gcggccttga gcgcgctttc catgcgctgg ctctggctga
     1021 tcggcacgac ggtgtcgtcc gtgccgtgga tcagcaggat cgggcaatag gccttcgccg
     1081 cctgtttcgc cggcgagatg gcgtcccagc cggacctgtc gcccagcacc cgccgccagg
     1141 cgacgacggc gggcgtgtcc atattgttgg tcgattccag caggtaactg ataatcgcgc
     1201 ccacatccga aggcccggcg atggagacgg cgcacctata gacgcccgga tcgagcgtgg
     1261 cgcccgccaa agccgcatag ccgccgtagg aggcgccgag aatggcgacg cgcttcggat
     1321 cggccagccc ctgcgcgacc agataacgca cgccgtcgga aagatcggtc tgcatcttgc
     1381 ggcccatctc gccgttgccc gcgtccatga aggcgatgcc ggcgccgccg gagccgcgat
     1441 aattgggttg cagcaccgcg tagccgcgcg aggccagcac ctgcgtctgc cagtcgaaat
     1501 cgacatggtc gcgcacccac gggccgccgt gcggcaacac gatcagcggc aggttcttcg
     1561 ccgggcgccc gttgagcgcc gcatccggcg gcagggtcag gtaggcgtga atcggcagcc
     1621 cgtcggcggc cttgtagtcg atcggctgtt tctgcgtgat ccacgccggc ggcagggcgg
     1681 cgtagttgga ggcgacgggc gatccgccgc ccgtggtgaa gtcgagatag acataggcgc
     1741 cggcgttgtg cgcgttctcg acatagacga tcatcttgcg cgggtcttcg gcgaagtccg
     1801 cgatgcggac ccggtcgccc ggatcgacga cctgcggcag ggcctcgacc agctttttca
     1861 gcagcggctc gaaatagtcg tcgcgctgcc agtcgtcgta atatctgaac ccggccagcc
     1921 tgcgcgtcac cggatggaac agggcgtcgc ggtccagggt cgcctgcggg tccagcgggt
     1981 cgctgacctt gccgtccgcc gagatttcgc ggaattgccc ttcgtaatcg cccttgtcga
     2041 aatagaccac gaccgacagc ccgtcgcggc cgacgccctg ggcgataggc aggctgaccg
     2101 ggtctttcac gccataggcg aactgaaaat gcggatcctt ttccgtcggc gtggcgttga
     2161 tatagagccg ccattcgttg tgcagcggga tatgttcgac ataggcgagc ggcgcgcctt
     2221 cggcggttac gataaaatcc gtcgcctcct gcgaggcctc gcagatgcgt cgcggcggcg
     2281 ccttgtccat gctgaagctg tagagataga acttgccgtc atagaccgag accgtgccgt
     2341 cgtccgtgtg caggcggtag ttggcggcgg tgacgcgata gcccgcaggc gtcttgacgc
     2401 gctgcaaacc gcccagcacc acgttggaga agccctccat attgctgaag aaggtcgtca
     2461 ccgcgcccgt gtcgacgtcg atgcagcgcg ccagcgtgaa ttcgtgccgg taaccggcga
     2521 aaccggtcag ggccgtggtc tgggaatcga ccagaatgac atgcgcattg tcgccccaga
     2581 acaggtcgcg caccttggcg ggaccgatgg agaggctttt cggctgcgga ttcgcgatct
     2641 cgtaatgcag cagaatcttc tcgtcgccct tttgcgagac gatggcgatg cgctggccgt
     2701 ccggcgacag ggcgatctgg tccaccagcg gcgaaccggc gtagatctcg atggcgggcg
     2761 ggccggacgc ggcgggcgcg gcttgcgccc cggcgaaacg cgcgccgacg gcgaacgcga
     2821 cggcgccggt cgccagaaag gcgcgacggc tggtctttga tgtcatgatg caggccccct
     2881 gttgttgcgt cgacgctaac
//
LOCUS       DASVLL010000579         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_149624,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000579 DASVLL010000000
VERSION     DASVLL010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149624"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..2122
                     /locus_tag="VF402_09520"
     CDS             5..2122
                     /locus_tag="VF402_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7800576.1"
                     /translation="MPPSPPRKPFRIEQLGRVREDAYHWMKDDNWQAVLRDPAKIRAD
                     VNAALDLENAYSAAVLAGTEGLQKQLFEEMKGRIKEDDASTPARDGPWEYYSRYDAGA
                     QHPILARRPYVARAFADPIPPVEATLADEQVLLDEAERAGGHDYYETSAAQHSPDHKL
                     FAWGEDTQGSEYYTVYVRDLETGEILPDPIEACEGGFCFSPDSQWIFWTWRDENSRPA
                     KIFRRPARGGADALVYTEEDEGFFLHVGVLSSEAFIAIGTGNHETSETWLIPAAHPAA
                     PPVCVARREDGVQYDVDHWSDRFVIRTNAGGAVDFKLMTSDAAHPSRETWRDLVPHRP
                     GVYLTGFDLSARHMIRIERENANTRILVTRKADMDEHVIAVDEEVYSLGGGGGYEYDS
                     PILRYAYTSPTTPAQTFDYDMETRQRVLRKTQVIPSGHDPAAYVARRLYATAPDGAQV
                     PVSVLMKKDTPLDGSAPLLLYGYGSYGMPMDPGFSIRSLSLVDRGWVYAIAHVRGGTE
                     KGYGWFLDGRKFKKTNTFSDFIACAEALCEAGYTRPGRLVAYGGSAGGMLMGAVANMR
                     PDLWAGVIGAVPFVDVLNTMSDASLPLTPPEWPEWGNPLEDAAAYDYIAGYSPYDNIT
                     PQPYPPVLATGGLSDTRVTYWEPMKWIAKLRETSTSDAPMLLKINMEAGHGGASGRFD
                     FLKEIAFDYAFAIWAQKRQQDGT"
BASE COUNT          396 a          748 c          698 g          345 t
ORIGIN      
        1 accgatcccg ccttcgccgc cgcgcaaacc cttccgcatc gagcaactgg ggcgcgtgcg
       61 cgaagacgcc tatcactgga tgaaggacga caactggcag gcggtgttgc gcgatccggc
      121 gaaaatccgg gccgacgtca atgccgcgct cgatctggag aacgcctaca gcgccgccgt
      181 gctggccggg accgaaggcc tgcaaaagca gcttttcgag gagatgaagg gccgcatcaa
      241 ggaggacgac gcctccacgc ccgccaggga cggcccgtgg gaatattaca gccgctacga
      301 cgccggggcg cagcatccga ttctggcccg ccgcccttac gtcgcgcgcg ccttcgccga
      361 tccgatcccg ccggtcgagg cgaccctggc cgatgagcag gtgctgctcg acgaggccga
      421 acgcgccggg ggccatgact attacgaaac ctccgccgcc cagcacagcc ccgatcacaa
      481 gctgttcgcg tggggcgagg acacgcaagg ctcggaatat tacaccgtct atgtccgcga
      541 tctcgaaacc ggcgagatcc tgcccgatcc gatcgaggcg tgcgaaggcg gcttctgctt
      601 ctcgcccgac tcgcaatgga tcttctggac gtggcgcgac gagaattcgc gcccggccaa
      661 gatcttccgc cgcccggcgc gcggcggcgc ggacgcgctg gtctataccg aggaggacga
      721 aggctttttc ctgcacgtcg gcgtgctgag ttcggaagcc ttcatcgcca tcgggaccgg
      781 caatcacgag acttcggaga catggctgat ccccgccgcg catcccgccg cgccgccggt
      841 ctgcgtggcg cggcgcgagg acggcgtgca atacgacgtc gatcactgga gcgaccgctt
      901 cgtcatccgc accaacgccg ggggcgcggt cgatttcaag ctgatgacct ccgacgccgc
      961 gcatccgtcg cgcgagacgt ggcgcgatct ggtcccgcac cgtcccggcg tttacctgac
     1021 cggcttcgac ctgtcggcgc ggcacatgat ccgcatcgag cgcgaaaacg ccaatacccg
     1081 catccttgtg acgcgcaagg ccgacatgga cgaacacgtc atcgcggtcg acgaggaggt
     1141 ttacagcctc ggcggcggcg ggggctacga atacgacagc ccgatcctgc gctacgccta
     1201 cacctcgccg accacgcccg cccagacctt cgactacgac atggagacgc gccagcgggt
     1261 cttgcgcaag acgcaggtca ttccgtccgg ccacgatccg gcggcctatg tcgcgcggcg
     1321 gctctatgca acggcgccgg acggcgcgca ggttccggtc agcgtgctga tgaagaagga
     1381 tacgccgctc gacggctcgg cgcccttgct gctgtacggc tacggctcct acggcatgcc
     1441 gatggacccc ggcttttcga tccgcagcct gagcctggtc gatcgcggct gggtttacgc
     1501 catcgcgcac gtgcgcggcg ggacggaaaa gggctacggc tggttcctcg acgggcgcaa
     1561 gttcaagaag accaacacct tcagcgactt catcgcctgc gccgaagccc tatgcgaagc
     1621 cggttatacc cgccccggcc gtctggtcgc ctatggcggc tcggcgggcg gcatgttgat
     1681 gggcgcggtc gccaatatgc gccccgacct gtgggcgggc gtgatcggcg ccgtgccgtt
     1741 cgtcgacgtg ctcaacacca tgagcgacgc ctccctgccg ctgaccccgc cggaatggcc
     1801 ggaatggggc aatccgctgg aggacgcggc cgcctacgac tacatcgccg gctacagccc
     1861 ctatgacaac atcacgccgc agccctatcc gcccgtgctg gcgaccggcg gcctcagcga
     1921 tacgcgcgtc acctactggg agccgatgaa atggatcgcg aaactgcgtg aaacctcgac
     1981 ctcggacgcg ccgatgctgc tgaagatcaa catggaggcc ggtcacggcg gcgcctccgg
     2041 ccgtttcgac ttccttaagg aaattgcctt tgattacgcc ttcgccatct gggcgcaaaa
     2101 aagacaacag gacgggacat gaccgagatc agggccgccg cggaagacga tttcgcggcg
     2161 attacgcagg tttatgcgca taatgtg
//
LOCUS       DASVLL010000580         5088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_254438,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000580 DASVLL010000000
VERSION     DASVLL010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254438"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF402_09525"
     CDS             complement(<1..822)
                     /locus_tag="VF402_09525"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7800577.1"
                     /translation="MPDPRFFDVGAKLDIDQAAALTQSRLAQPDAKGSGFIEACAPLS
                     FSGDAGLGAGRAAYFSDRRYLAQLHRTAAEYVFVTPDFAGHVPASSVALICAQPQAAW
                     SRVAGRLYRPRVHAGDKAIHPSARLEDGVQLGVGVVIGQDVEIGAGTRIEAYAVIGPG
                     CRIGRDGRVGAHATVFCALVGDRVQLASGARIGEAGFGVSGDARGLVDVPQLGRVILQ
                     DDVSIGAGTCVDRGAFDDTVIGEATKIDNMVQIAHNVRLGRSCIVAAHSGLSGSVV"
     gene            complement(838..1539)
                     /locus_tag="VF402_09530"
     CDS             complement(838..1539)
                     /locus_tag="VF402_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX7800578.1"
                     /translation="MTKATLISLGALILAGVSGQALAQAAAPAAPAAPTPPTFGAAIP
                     GQCVLDEQTAMADSAMGKAAADRLRQLKAVVDSELSTEGKSLDDERQQLAQQQKLATT
                     PAAKTAFETKAQAWEQKRQAFQQKVEQRNQEMQYTQQEVMSAIFQKMIPQINAVVTQK
                     SCATVISADSLLHYDMTSNTNGQSSQTSFLYANPAMNITQDVVAKLDATKELLPQFDR
                     VSLDQPAQGAAPAKK"
     gene            complement(1592..3931)
                     /gene="bamA"
                     /locus_tag="VF402_09535"
     CDS             complement(1592..3931)
                     /gene="bamA"
                     /locus_tag="VF402_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX7800579.1"
                     /translation="MIVTFSVRWLAASTRAAKAAPQAEPAAANPTARVDRFVVSGNER
                     IDSQTIISYLPFQTGAVVDSEMIDLAVKTLYNTDLFSDVQIVMNGNTMDITVVESPII
                     NQVVFEGNHAMTKDKLRDEVQIRPRGVFTKAKVQADVQRIIELYRKSGRIAATVTPKI
                     VELPQKRVDLIFEINEGPKTGVSSVNFIGNHAFSDSELQGVVVTKKSLWWKFFSSNDN
                     YDPDRMDYDREQLRKYYTNRGYYDFRVISAVAELTPDRKNFAISYTLDEGQKYSFGRI
                     TIKTDNDKLKAENLQRAISIRPGQLYESDRVDKAVDALTFAAGSNGYAFVDIRPDEEA
                     NPNTKTVDLTFNVHEGARVYIDKINIVGNTQTLDKVIRRQLLVSEGDAYNKALLERSK
                     MYIAGLGFFKQGDDGVKITEKPSPEPGKTNIEVAVQEQPTGELSFGAGFSSYEKFILD
                     IGVSQSNFRGTGQEVRARVQTGSIQKDIDFSFTEPHFMGRDVSAGFDLFESSYNYTGV
                     DYSQDSRGGDVRLGYILNGYSRLNLRYNIRKDDITYTSSDSCNGLLYNCGSGITSSVG
                     YTLGFDLRNDYVQPTRGWTAYLRQDFAGLGGNVKYVDTELEAHWYHGFAKDMVLAVSG
                     NAGTKTPWGGDSIRINDRFFKGGDTIRGFEYAGIGPRDTSTGYALGGQTYAIGSVELG
                     VPNHLPDQYGLKTAVFIDVGTLGGVDKRLKINTTPGSSTAGQRLTNIVDDFGLRASAG
                     VTIRWKSPMGPVQFDLSQVLAKDKYDKVETFRFSQSTQF"
     gene            3879..4769
                     /locus_tag="VF402_09540"
     CDS             3879..4769
                     /locus_tag="VF402_09540"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX7800580.1"
                     /translation="MAARVDAASHLTLNVTINGQGPFRFVVDTGAERSVIADNVAAAL
                     QLPVGPSVTVDGISSRVSAPSVRVERLAFGPFTRRLVMPVLPRLNLQSDGYLGLDALS
                     DARVTFDFAHRQVRIDERSVISPHYALLPRDASHDDAPPDTSAVVRAHGKDGHLRVTD
                     SRVDSVVATAFVDTGAEVSVGNPALYDALKQRNKMLTELGAVMLSGVTGGQLPGVVIP
                     VRRIHLQDILFSDGVLVVADPPDFTTWRLNHRPALLIGLDYLRQFAAVTIDYRDQAMR
                     FELAAVTPHPTPAVSIEQMG"
     gene            complement(4773..>5088)
                     /locus_tag="VF402_09545"
     CDS             complement(4773..>5088)
                     /locus_tag="VF402_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7800581.1"
                     /translation="PGLSEDRAEFGEIVVDRMSHRVQRSGRDIHLGPTEYKLLDYLMQ
                     YPRRVFSREQLLDAVWGNDVYVEARTVDVHIGRLRKALNGKDDIDPIRTVRSAGYALD
                     LG"
BASE COUNT          810 a         1635 c         1687 g          956 t
ORIGIN      
        1 gaccaccgaa ccggaaagcc ccgaatgcgc cgccacgatg caactgcggc ccagacgcac
       61 attgtgcgcg atctggacca tgttgtcgat cttcgtcgct tcaccgatca ccgtgtcgtc
      121 gaaggcgccg cgatccacgc aggttccggc gccgatcgac acgtcgtcct gaaggatgac
      181 gcgccccagt tgcggcacgt cgaccagccc gcgcgcgtcg ccgctgacgc cgaatccggc
      241 ctcgccgatg cgcgcgcccg aagccagttg cacccggtcg cccaccagcg cgcaaaagac
      301 ggtggcgtgc gcgccgacgc ggccgtcgcg gccgatgcgg cagcccggcc cgatcacggc
      361 gtaggcctcg atgcgcgttc cggcgccgat ctcgacgtcc tgaccgatca ccacgccgac
      421 gccgagttgc acgccgtcct ccagacgcgc cgacggatgg atggccttgt cgcccgcatg
      481 cacgcgcggg cgatagagcc gcccggccac gcgcgaccac gccgcctgcg gctgggcgca
      541 gatcagcgcg acgctggacg ccgggacatg accggcgaaa tcgggggtga cgaagacata
      601 ttccgccgcc gtgcggtgca gttgcgccag atagcgccgg tcgctgaaat aggccgcgcg
      661 cccggcgccc agaccggcgt cgccggaaaa cgacaaaggg gcgcaggctt cgatgaagcc
      721 gctccccttc gcgtccggct gcgccagccg gctttgcgtc agcgccgctg cctgatcgat
      781 gtcgagtttt gctccgacgt caaaaaaacg gggatcaggc atatagcggt ttaactctta
      841 cttcttggcg ggcgcggccc cctgagccgg ctgatccagg ctcacgcggt cgaactgcgg
      901 caggagttcc ttggtcgcgt ccagcttggc caccacgtcc tgcgtgatgt tcatggccgg
      961 gttggcgtag aggaacgagg tttgactcga ttggccattg gtattcgacg tcatgtcata
     1021 atgcaacagg ctgtccgccg agatgaccgt cgcgcagctt ttttgcgtca ccacggcgtt
     1081 gatctgcgga atcatcttct ggaagatggc cgacatgact tcctgctggg tgtactgcat
     1141 ttcctggttg cgctgctcga ccttctgctg gaaggcctga cgcttctgct cccaggcctg
     1201 cgccttggtt tcgaacgcgg tcttggccgc cggggtggtg gccagcttct gctgttgcgc
     1261 cagttgctga cgctcgtcgt ccagcgactt gccttcggtc gacagctcgc tgtccaccac
     1321 ggccttcaac tgacgcaggc ggtccgccgc cgccttgccc atggcggaat cggccatggc
     1381 ggtctgttcg tccagcacgc actggcccgg aatggcggcg ccgaaggtcg gcggggtcgg
     1441 cgcggcgggc gcggcgggcg cggccgcctg cgccagggcc tgaccggaaa cgcccgccag
     1501 aatcagggcg ccgagcgaaa tgagagtagc ttttgtcatc tgtgtttctg tccgtttttg
     1561 gaaagcggtc ttcgttgtga agacgggttt atcagaattg ggtggattgc gagaagcgga
     1621 aggtttcgac cttgtcgtat ttatccttgg ccagaacctg gctgaggtcg aattgcaccg
     1681 gtcccatcgg cgacttccag cggatcgtaa cgcccgccga agcgcgcagt ccgaagtcgt
     1741 cgacgatatt ggtcagacgc tggcccgccg tgctggagcc cggcgtggtg ttgatcttga
     1801 gacgcttgtc gacgccgccc agcgtgccga cgtcgatgaa caccgccgtt ttcaggccgt
     1861 actggtccgg cagatggttc gggacgccca gttcgaccga gccgatggcg taggtctggc
     1921 cgcccagcgc atagccggtc gaggtgtcgc gcgggccgat gcccgcgtac tcgaagccgc
     1981 gaatggtgtc gccgcccttg aagaaacggt cgttgatgcg gatggaatcg ccgccccacg
     2041 gcgtcttggt gcccgcattg cccgacacgg ccagcaccat gtccttggcg aagccgtggt
     2101 accagtgcgc ctcaagctcg gtgtcgacat atttgacgtt gccgcccaga cccgcgaaat
     2161 cctgacgcag ataggcggtc cagccgcgcg tcggctgcac gtagtcgttg cgcaggtcga
     2221 agcccagcgt atagccgacc gacgaggtga tgccggagcc gcaattgtac agaaggccgt
     2281 tacagctgtc cgagctggtg taggtgatgt cgtccttgcg gatgttgtag cgcaggttca
     2341 accggctgta gccgttcagg atatagccga ggcgcacgtc gccgccgcgc gaatcctgcg
     2401 aatagtccac gccggtgtaa ttgtacgagc tttcgaacag gtcgaaaccg gcggacacgt
     2461 cgcggcccat gaagtgcggc tcggtgaacg agaagtcgat gtccttctgg atcgagccgg
     2521 tctggacgcg cgcgcgcacc tcctggccgg tgccgcggaa gttggactgc gacacgccga
     2581 tgtcgaggat gaacttttca taggacgaga agcccgcccc gaagctcagc tcgccggtcg
     2641 gctgctcctg cacggcgacc tcgatattgg tcttgcccgg ctccggcgac ggcttttcgg
     2701 tgatcttgac gccgtcgtcg ccctgcttga agaagccgag gccggcgata tacatcttgg
     2761 agcgctccag cagcgccttg ttgtaggcgt cgccttcgga caccagcaac tgacggcgga
     2821 tcaccttgtc cagggtctgc gtgttgccga cgatgttgat cttgtcgata tagacgcgcg
     2881 cgccttcatg cacgttgaag gtcaggtcga ccgtcttggt gttcggattg gcctcctcgt
     2941 cgggacggat gtcgacgaag gcgtagccgt tcgagcccgc cgcgaaggtc agggcgtcca
     3001 ccgccttgtc gacgcggtcg ctttcataaa gctggcccgg ccggatcgag atggcgcgct
     3061 gcaggttctc ggccttcagc ttgtcgttgt cggtcttgat ggtgatgcgg ccgaagctgt
     3121 acttctggcc ttcgtccagc gtgtaggaga tcgcgaaatt cttgcggtcg ggcgtcagct
     3181 ccgccaccgc cgagatcacg cggaaatcgt aatagccgcg gttggtgtaa tacttgcgca
     3241 actgctcgcg gtcgtagtcc atccggtcgg gatcgtagtt gtcgttcgac gagaagaact
     3301 tccaccacag ggatttcttg gtgaccacga cgccctgaag ctcgctgtcc gaaaaggcgt
     3361 ggttgccgat gaagttgacg ctggacacgc cggtcttggg gccttcgttg atctcgaaga
     3421 tcaggtcgac gcgcttttgc ggcagttcga cgatcttggg cgtgaccgtc gcggcgatgc
     3481 ggcccgactt gcggtacagc tcgatgatgc gctgcacgtc ggcctgcacc ttggccttgg
     3541 tgaacacgcc gcgcggacgg atctgcacct cgtcgcgcag cttgtccttg gtcatggcgt
     3601 ggttgccctc gaacaccacc tgattgatga tcgggctctc gaccaccgtg atgtccatcg
     3661 tattgccgtt catcacgatc tgcacgtcgg agaacaggtc ggtgttgtac agcgtcttga
     3721 ccgccaggtc gatcatctcg gaatcgacga cggcgccggt ctggaacggc aggtaggaga
     3781 tgatcgtctg cgaatcgatg cgctcgttgc cgctgaccac gaagcggtcg acgcgcgcgg
     3841 tcggattggc ggccgcgggt tcggcctgag gcgcggcctt ggccgcccgc gtcgacgccg
     3901 ccagccacct gacgctgaac gtgacgatca acggacaagg cccgttccgc ttcgtcgtcg
     3961 acaccggcgc ggaacgttcg gtcatcgccg acaatgtcgc cgccgcgctg caactgccgg
     4021 tcgggccgag cgtgaccgtg gacggcattt ccagccgggt cagcgcgccc agcgtgcggg
     4081 tcgagcgcct cgccttcggc ccgttcacgc ggcggctggt catgccggtg ctgccgcggc
     4141 tcaacctgca atcggacggc tatctggggc tggacgcgct cagcgacgcg cgcgtgacct
     4201 tcgacttcgc gcaccggcag gtgcgcatcg acgagcgcag cgtcatcagc ccgcactatg
     4261 cgctcctgcc gcgcgacgcc tcgcacgacg acgcgccccc ggacacctcc gccgtggtgc
     4321 gcgcgcacgg caaggacggc cacctgcgcg tcaccgacag ccgcgtggac agcgtggtcg
     4381 ccaccgcctt cgtcgacacc ggcgccgagg tgtcggtcgg caatccggcg ctgtacgacg
     4441 cgttgaaaca gcgcaacaag atgctgaccg agctgggcgc cgtgatgctg agcggcgtga
     4501 cgggcggcca gttgcccggc gtcgtcatcc cggtccggcg cattcatttg caggatatcc
     4561 tgttcagcga cggcgtgctg gtcgtcgccg atccgcccga tttcaccacc tggcggctga
     4621 accaccgccc ggccctgctg atcgggctgg actatctgcg ccagttcgcg gcggtgacga
     4681 tcgattatcg cgatcaggcg atgcgcttcg aactggccgc cgtcacgccg catccgacgc
     4741 cggcggtgag catcgagcag atgggataac gcttagccga ggtcgagcgc gtagccggcg
     4801 gagcgcacgg tgcggatcgg atcgatgtcg tccttgccgt tcagcgcctt gcgcaggcgg
     4861 ccgatatgca cgtcgaccgt gcgcgcctcg acatagacgt cgttgcccca gaccgcgtcc
     4921 agcagttgct cgcgcgaaaa gacgcggcgg ggatactgca tcaggtagtc gagcagcttg
     4981 tattccgtcg ggccgaggtg gatgtcgcgg cccgaccgct gcacgcggtg cgacatgcgg
     5041 tcgacgacga tctcgccgaa ctcggcgcgg tcctcggaca ggccgggg
//
LOCUS       DASVLL010000581         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_38597, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000581 DASVLL010000000
VERSION     DASVLL010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38597"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1664
                     /gene="argJ"
                     /locus_tag="VF402_09550"
     CDS             249..1664
                     /gene="argJ"
                     /locus_tag="VF402_09550"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX7800582.1"
                     /translation="MARPPASDAPSTSAKIRAAQGGLERALDPLAKAVKRAVQPVADL
                     AGHLTPGGDAPAPGKPGLDVSPLAVPLPVMPPLAGARLATARSGFYKHERKDTLLMVF
                     DEGTTCAGVFTRHTVGSAPVDWCKKQLAANEGADVRALIVNAGCANAFTGKAGADAAR
                     RTASALARRIDCRQRDIMLASTGVIGVVLDDAKIAARIPQLEADLRADAWADAAATIM
                     TTDTFPKGASETAEIDGVQVRINGIAKGSGMIAPDMATMLAFIVTDATLSPGVLQSLL
                     ALYTRTTFNSVTVDGDTSTNDTCLLFATGAAGAPRIARAGDRRLADFKAKLEKVMHNL
                     ATQLVRDGEGARKFVKVNVTGAASPASARRIAKSIAESPLVKTAIAGEDANWGRIVMA
                     VGKTEEPVEREHLAIRFGDLVAAENGAVARGYDEAAMSAYMKNAELEISVDVGVGRAS
                     AHVYTCDLTHGYISINGDYRS"
     gene            complement(1677..2060)
                     /locus_tag="VF402_09555"
     CDS             complement(1677..2060)
                     /locus_tag="VF402_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800583.1"
                     /translation="MCLALAACSKPAPPPPPPAAPPPAPAYDPWPGRYEGDLMARISP
                     LHRVTLVTARDGCTGDAGLAGGEPSQTVGADHLRVTLTAKATGAVCTVDLRRNGDRLT
                     VSESGDCAAWHGAACPFAGVAARVK"
     gene            complement(2078..>2341)
                     /locus_tag="VF402_09560"
     CDS             complement(2078..>2341)
                     /locus_tag="VF402_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7800584.1"
                     /translation="AQESLATYGGGFAATSLNTLVQMVGSGLGVSLLPAMAVEAGLAR
                     DGGISVRPLASDHAFRDIAVVWRAGSSHAAEARLLAEQFKADA"
BASE COUNT          370 a          860 c          802 g          309 t
ORIGIN      
        1 ggccaagacg ccggataagg cttcgggcaa accgcccgaa ctgctcaaga gcgatccgct
       61 gccgccgggc catcactttt tcgcgccgcc ccggctgaag aagaactgac tgtccgcgtc
      121 ccggattcct aatgcaacgg cattggcgaa gcggtccggc tctgataagg gctgagcgat
      181 cgcccgtgct taccgcccgt gcttaccgcc cgtgcttacc gcccgtgctt accgatggag
      241 tcccgctcat ggccagaccg cccgcttccg acgccccttc gacctccgcg aaaatccgcg
      301 cggcgcaagg cgggctggag cgcgcgctcg acccgctggc caaggcggtc aagcgcgccg
      361 tgcagccggt cgccgatctg gccggtcacc tgacgccggg cggcgacgcg cccgcgccgg
      421 gcaagccggg cctcgacgtc tcgcctctgg ccgtgccgct gcccgtcatg ccgcctctgg
      481 ccggggcgcg gctggcgacg gcgcggtcgg gcttctacaa gcatgagcgc aaggacacct
      541 tgctgatggt cttcgacgaa gggacgacct gcgcgggcgt gttcacgcgc cacacggtcg
      601 gctcggcgcc ggtggactgg tgcaagaagc aactggccgc caacgaaggc gcggacgtgc
      661 gcgcgctgat cgtcaacgcc ggttgcgcca acgccttcac cggcaaggcc ggggccgacg
      721 ccgcccgccg caccgcctcc gcgctggcgc ggcgcatcga ttgccgtcag cgcgacatca
      781 tgctggcctc gaccggggtg atcggcgtgg tgctggacga cgcgaagatc gccgccagga
      841 ttccgcagct cgaagccgac ctgcgcgccg acgcctgggc cgacgccgcc gcgaccatca
      901 tgaccaccga caccttcccc aagggcgcgt cggaaaccgc cgagatcgac ggcgttcagg
      961 tgcgcatcaa cgggatcgcc aaggggtcgg gcatgatcgc gccggacatg gcgaccatgc
     1021 tggccttcat cgtcaccgac gccaccctgt cgcccggcgt gctgcaaagc ctgctggcgc
     1081 tctatacgcg caccaccttc aattcggtga ccgtggacgg cgacacctcg accaacgaca
     1141 cctgccttct gttcgccacc ggcgctgcgg gtgcgccgcg catcgcgcgc gcgggcgacc
     1201 ggaggctggc cgacttcaag gccaagctgg aaaaggtcat gcacaacctc gccacgcaac
     1261 tggtgcgcga cggcgagggc gcgcgcaaat tcgtcaaggt caacgtcacc ggcgccgcca
     1321 gcccggcctc ggcgcgccgg atcgccaagt cgatcgccga aagcccgctg gtcaagaccg
     1381 ccatcgcggg cgaggacgcc aactggggcc gcatcgtcat ggcggtcggc aagaccgagg
     1441 agccggtcga gcgcgagcat ctggcgatcc gcttcggcga tctggtcgcg gcggagaacg
     1501 gcgccgtggc gcgcggctac gacgaggcgg cgatgagcgc ctacatgaag aacgccgaac
     1561 tggagatcag cgtcgatgtc ggcgtcggcc gcgcctcggc gcacgtctat acctgcgacc
     1621 tgacgcacgg ctatatcagc atcaacggcg attaccggtc gtaatccgca ggcgcctcac
     1681 ttcacgcgcg cggcgacgcc ggcaaagggg caggccgccc catgccacgc cgcgcagtcg
     1741 ccgctttcgc tcaccgtcag ccggtcgccg ttgcgcctca ggtcgaccgt gcagaccgcg
     1801 cccgtcgcct tggcggtcag ggtcacgcgc agatgatcgg cgccgaccgt ctgcgacggc
     1861 tcgccgcccg cgaggcccgc atcgccggtg cagccgtccc tggcggtgac cagcgtgacg
     1921 cgatgcagcg gcgaaatccg cgccatcagg tcgccctcgt accggcccgg ccacggatcg
     1981 taggcgggcg cgggcggcgg cgcggcgggc ggcggcgggg gcggcgcggg tttcgagcag
     2041 gcggccaagg ccagacacat aataagaacc gcccgcttca tgcgtccgcc ttgaactgtt
     2101 cggccagcag ccgcgcctcg gcggcgtggc tggaccccgc gcgccagacg acggcgatgt
     2161 cgcggaaggc gtgatcggag gccagaggcc gcacgctgat cccgccgtcg cgcgccagcc
     2221 cggcctcgac cgccatggcg ggcagcagcg acacgcccag ccccgaaccg accatctgca
     2281 ccagcgtgtt gagcgaggtc gccgcgaagc cgccgccata ggtggccagc gattcctgcg
     2341 c
//
LOCUS       DASVLL010000582         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_150145,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000582 DASVLL010000000
VERSION     DASVLL010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150145"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..744)
                     /locus_tag="VF402_09565"
     CDS             complement(193..744)
                     /locus_tag="VF402_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800585.1"
                     /translation="MADPKKPAADGVADRNVDQIRDILFGGQMRDYERRFQELNQRLE
                     ADLARQRDMQDKRLAQIDRRVDDQLEKLGKLMRQEVQDRNGAVDDLESRLQQAARTSR
                     AEINKAMEALTQELAATDERLRAALADLHGVASARVAEAESALSRASTELRAEKVGRD
                     DLAALLTEFALRLKGDFDLPTPK"
     gene            complement(958..1656)
                     /locus_tag="VF402_09570"
     CDS             complement(958..1656)
                     /locus_tag="VF402_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7800586.1"
                     /translation="MSRLGLVVGVLEDDPDMAVLVTQWLEHAGYAVRWFRSATEYRRR
                     QGSESIDLLLLDWMLPDGDGVEVLGAIRASANATLPVIFLTARDGEDDLVRGLASGGD
                     DYVVKPPKERELVARVDAVLRRRAGGADSDGTFELAPYSVDMKRRRVSLDGREIDLTQ
                     REFDLATFLFRRHGRIVSREALLENVWNLSAVVSTRTVDTHVSRLRKKLDLNGSNGWR
                     LAAIYQHGYRLEQV"
     gene            complement(1699..>1845)
                     /locus_tag="VF402_09575"
     CDS             complement(1699..>1845)
                     /locus_tag="VF402_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HEX7800587.1"
                     /translation="AVQSGLRKVSQTVKNIKGAWVNEIKVATGPDGNVVEWRVNMRIN
                     FIVD"
BASE COUNT          234 a          656 c          636 g          319 t
ORIGIN      
        1 gcgcgcgcgc gccgcgtcga gcgcgcggcg atcgtcgccg acgatcagct cgcgcaggcg
       61 ctcgaagtcg tcgtcgtgca gcgcggccgc ttcggcggtc tgctccggcg cgatcacctc
      121 agcggatggc ggcccgcccc atgcgctgcc ggatccgctc gtcgtcatgt ccgcgtcctg
      181 tccgccgccg cgtcacttcg gcgtcggcag atcgaagtcg cctttcaggc gcagcgcgaa
      241 ctcggtcagc agcgcggcga gatcgtcgcg gccgaccttc tcggcgcgca gttcggtgct
      301 cgcgcgcgac agcgcggatt cggcctcggc gacgcgcgca ctcgccacgc cgtgcaggtc
      361 ggcgagtgcg gcgcgcagcc gctcgtcggt ggctgcgagt tcctgtgtca gcgcctccat
      421 ggccttgttg atctcggcgc gcgaggtgcg cgcggcctgt tgcaggcgcg actcgaggtc
      481 gtccaccgcg ccgttgcggt cctgcacctc ctggcgcatg agcttgccga gtttctcgag
      541 ctggtcgtcg acgcgcctgt cgatctgcgc gaggcgcttg tcctgcatgt cgcgctggcg
      601 cgcgaggtcc gcctccagcc tctggttcag ttcctggaag cggcgctcgt agtcgcgcat
      661 ctggccgccg aacaggatgt cgcggatctg gtcgacgttg cgatcggcga cgccatccgc
      721 ggcgggcttc ttcgggtctg ccatgggctt gctcctggtg tgcgtgcgcg ccgggacagc
      781 cttgcgcggc gcagccggcg gacgttgccg ctggccggtc ttcttgcgtg gaagcggctt
      841 tgctgcggtc gccacggcgt cccccggaaa tatacggtct gcgcggcatt cttgcactcc
      901 gcgcacgctg ctgcacgcac cgtccgccgc accgggaaga cgcccggcga aggcgcgtca
      961 gacctgctcc agccggtagc cgtgctggta gatcgcggcc aggcgccagc cgttgctgcc
     1021 attgaggtcg agcttcttgc gcaggcggct gacatgggta tcgaccgtgc gcgtggacac
     1081 caccgcgctg aggttccaga cattctcgag cagcgcctcg cggctgacga tgcggccgtg
     1141 gcgccggaac aggaacgtgg cgagatcgaa ctcgcgctgc gtcaggtcga tctcgcgccc
     1201 gtcgaggctg acccggcgcc gcttcatgtc gaccgaatac ggcgccaact cgaacgtgcc
     1261 gtccgaatcc gcgccgccgg cccgccggcg cagcaccgca tcgacgcgcg cgaccagctc
     1321 gcgctccttc ggcggcttca cgacgtagtc gtcgccgccg ctggcgaggc cccgcacgag
     1381 gtcgtcctcg ccgtcgcggg cggtcaggaa gatcaccggc aacgtcgcat tcgccgatgc
     1441 gcggatcgcg ccgaggactt cgacgccgtc gccgtcgggc agcatccagt cgagcagcag
     1501 caggtcgatc gactccgaac cctggcgccg gcggtattcg gtggcgctgc ggaaccagcg
     1561 caccgcgtag ccggcatgtt ccagccattg cgtgaccagg actgccatgt cggggtcatc
     1621 ctcgagcacg ccgacgacca ggccgagcct gctcatggcg tcggcagccg gctgccggaa
     1681 cgcgcgttcg gcgacacgtc agtcgacgat gaaattgatc cgcatgttga cgcgccactc
     1741 gaccacgttg ccgtccgggc cggtggcgac cttgatttcg ttgacccagg cgcccttgat
     1801 gttcttgacg gtctgggaaa ccttcctgag cccgctctgt accgc
//
LOCUS       DASVLL010000583         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_38781, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000583 DASVLL010000000
VERSION     DASVLL010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38781"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VF402_09580"
     CDS             complement(<1..1356)
                     /locus_tag="VF402_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7800588.1"
                     /translation="MRNTVLNSLMTATALATVAVMSAYAPASAQTQMTTVARQVASNP
                     NIAAKDAPDPRAYLDDIEGEKALAWVRTHNQATFDKLEKDPRFAPAQADMLKILQATD
                     RIAAPGFAHKGMIDNFWQDGTHVQGLWRRTTWESYRSGHPKWRTILDLDALSKAEGRT
                     WVFHGADCLPPAYNRCLISLSDGGKDADVVREFDIAARAFVKDGFALPEGKQTATWRD
                     ADTLYVTREWAPGEVTASGYAYVTRALKRGQSLDQAVEIFRGDKADVSAGRGVLRDIG
                     GDYVMDTSYRGLDFFNTEQAFYPNGRKVVLPLPTTAAFSAYYKGQAVYQLKSDWTSAK
                     GTVFRNGAVIAFDLKAALADPAHIEPTTLFMPNAHQSVESVSQTKTRLILSILSNVTG
                     EARSFDFGPKGWSSHLLALPENSTLSVTSTDDESDRLFVFSEGFLEPSTLYRADAASG
                     AI"
     gene            1529..2386
                     /gene="phhA"
                     /locus_tag="VF402_09585"
     CDS             1529..2386
                     /gene="phhA"
                     /locus_tag="VF402_09585"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX7800589.1"
                     /translation="MRSEEDRRGGVGGHAPERPDWITPQDWDGYTPQEHGVWRTLFER
                     QIGLLPGRASQHYLDGLNGLPIGPEAIPDFEALSEVLTRRTGWRVVAVPGMVPNDVFF
                     DHLAHRRFPAGRFIRTPAQLDYLQEPDVFHDIFGHAPMLMNPVMADFMQAYGEGGLRA
                     LKLGALERIARLYWYTVEFGLIAEAGDARILGAGIASSFTETLYALESPSPRRIGFDL
                     RRVMRTHYRIDALQENYFVLPSLDVLFDLADTDFGPVYEELAGMPDIQPPAILPEDRV
                     FMRGVGLER"
     gene            complement(2434..3183)
                     /gene="mtgA"
                     /locus_tag="VF402_09590"
     CDS             complement(2434..3183)
                     /gene="mtgA"
                     /locus_tag="VF402_09590"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX7800590.1"
                     /translation="MTDQPEDAIPGLGEEVIYDAVPHDRVMRKRFRPLRFLGLLILGF
                     FVFSTLSVAIHRFVPVPVTILMIERLAQGQGLDHRWVPASRISDNLKAAVIASEDAKF
                     CTHDGFDYDAIRKAEAYNATHKKKRGASTISQQTAKNVFLWPSRSWVRKGFEVWYTFL
                     IEHMWSKDRIMEVYLNSIEMGPGVYGAEAGSQYWFGHSAARLSPAEAARLAAILPKPL
                     SWKAKGGKRSGRIQSRARLVDRQGLDVCAAE"
     gene            complement(3203..>3600)
                     /locus_tag="VF402_09595"
     CDS             complement(3203..>3600)
                     /locus_tag="VF402_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX7800591.1"
                     /translation="DSKDMRMGGYPAKIKSMGFFAKGKLFATSGAMGAVVWPFLKPTG
                     PMGEEASEINPQEATLVSLVAGAPQDTLLAAGTEDGRVWLAELQSTGVDWVKAEKGAP
                     ITALALSNEANRLLIGDEDGYVYIFEAEA"
BASE COUNT          583 a         1115 c         1242 g          660 t
ORIGIN      
        1 gatcgcgccg ctcgccgcgt cggcccggta aagggtcgag ggttctaaaa agccttcgga
       61 aaagacgaac aggcggtcgc tctcgtcgtc cgtggaggtc accgacaggg tcgaattttc
      121 cggcagggcc agcagatgcg acgaccagcc tttcgggccg aaatcgaagc tgcgcgcctc
      181 gcccgtgacg ttggacagga tcgacaggat caggcgggtc ttggtctggc tgacgctctc
      241 gaccgactgg tgcgcgttgg gcatgaacag agtcgtcggc tcgatatggg ccggatcggc
      301 cagcgcggcc ttcaggtcga aggcgatgac ggcgccgttg cggaagacgg ttcctttcgc
      361 cgacgtccag tcggacttga gctggtacac cgcctgaccc ttgtaatagg cgctgaaggc
      421 ggcggtggtc ggtaagggca ggacgacctt ccggccgttc ggatagaagg cctgttcggt
      481 gttgaagaag tcgaggccgc gatacgaggt gtccatgacg taatcgccgc cgatgtcgcg
      541 cagcacgccg cgcccggcgg agacgtcggc cttgtcgccg cggaagatct cgaccgcctg
      601 atcgaggctc tggccgcgtt tcagcgccct ggtgacgtag gcgtagccgg aggcggtgac
      661 ctcgcccggc gcccattcgc gcgtgacgta aagcgtgtcc gcgtcgcgcc atgtcgccgt
      721 ctgcttgcct tcgggtaagg caaagccgtc cttgacgaag gcgcgtgcgg cgatgtcgaa
      781 ttcgcgcacc acgtcggcgt ccttgccgcc gtcggacagg ctgatcaggc agcggttgta
      841 tgcgggcggc aggcagtcgg cgccgtggaa cacccatgtc ctgccctccg ccttcgacag
      901 cgcgtcgagg tcgaggatcg tccgccactt cggatggccg gagcggtacg attcccacgt
      961 cgtgcgccgc cacaggccct gcacatgggt tccgtcctgc cagaaattgt cgatcatgcc
     1021 cttatgcgcg aagcccggcg cggcgatgcg gtcggtcgcc tgcaaaatct tcagcatatc
     1081 ggcttgcgcg ggcgcgaagc gcgggtcttt ctccagctta tcgaaggtcg cctgattgtg
     1141 cgtcctgacc caggccagcg ccttctcgcc ctcgatatcg tcgagatagg cgcgcgggtc
     1201 gggcgcgtcc ttggcggcga tgttcggatt ggaggcgacc tgccgggcga cggtcgtcat
     1261 ctgggtctgg gcggaggcgg gggcgtaggc ggacatgacc gccaccgtag ccagcgccgt
     1321 ggccgtcatc aagctgttca ggactgtgtt gcgcatggat cgccccgccg acgctacaag
     1381 atacgattga aactaacccg tttcgcggcg ctggcaagcg cgggccggat tacaaaatgt
     1441 tcatgttgcg cgcgccgctt cacggcgatg atatttgcat ttgcaaccac atcggcgcat
     1501 cctgacgcga accggcaagg agcgtccgat gcggagcgag gaagacaggc gcggcggggt
     1561 cggcggtcac gcgccggagc ggcccgactg gatcacgccg caggactggg acggctacac
     1621 gccgcaggag catggcgtgt ggaggacgct gttcgagcgc cagatcgggc tgcttccggg
     1681 ccgcgcttcg cagcattatc tcgatggcct gaacgggctg ccgatcgggc cggaggcgat
     1741 ccccgacttc gaggcgctgt ccgaggttct gacgcggcgc accggctggc gcgtggtcgc
     1801 cgtgccgggc atggtcccga acgacgtgtt tttcgaccat ctggctcacc gccgttttcc
     1861 cgccggacgg ttcatccgca cgcccgccca gttggattat ttgcaggagc cggacgtctt
     1921 tcacgacatt ttcggccatg cgccgatgct gatgaatccg gtgatggccg acttcatgca
     1981 ggcctatggc gaaggcggcc tgcgcgcgct gaagctgggc gcgctggagc gcatcgcgcg
     2041 gctctactgg tacacggtcg agttcgggct gatcgcggag gcgggggatg cgcgcatcct
     2101 cggcgccggg atagcgtcgt ccttcaccga aacgctgtac gcgctggaaa gcccgtcgcc
     2161 gcgccgcatc ggcttcgatc tgaggcgcgt gatgcgcacc cattaccgca tcgacgcgtt
     2221 gcaggaaaac tacttcgtgc tgccgtcgct ggatgtgctg ttcgatctgg ccgatacgga
     2281 tttcgggccg gtttatgagg aactggcggg tatgccggat atccagccgc ctgcgatctt
     2341 gccggaggac agggtgttta tgcggggcgt ggggttggag aggtaagata ccccgccacc
     2401 gcatcgcgct gtgctcgtgc ggatgaggaa gggctattcc gccgcgcaga cgtcgaggcc
     2461 ctggcggtcg acgaggcggg cgcgggactg gatgcggccg gagcgtttgc cgcctttcgc
     2521 cttccacgac aggggcttgg gcaggatggc ggccagccgg gcggcttcgg cggggctgag
     2581 cctcgccgcc gaatggccga accagtattg cgacccggcc tcggcgccgt aaacgcccgg
     2641 ccccatctcg atcgagttga gatagacctc catgatgcgg tccttggacc acatatgctc
     2701 gatcaggaag gtgtaccaga cctcgaagcc cttgcgcacc cacgagcggc tgggccacag
     2761 aaagacgttc ttggccgtct gctgcgagat ggtcgaggcg ccgcgcttct ttttgtgcgt
     2821 ggcgttatag gcttcggcct tgcggatcgc gtcgtagtcg aagccgtcat gggtgcagaa
     2881 tttcgcgtcc tccgaggcga tgacggcggc cttgagattg tcggaaatcc gcgacgccgg
     2941 aacccagcgg tgatcgaggc cctggccctg cgccagccgc tcgatcatca ggatggtcac
     3001 cggcacggga acgaagcggt ggatcgccac cgacagggtc gaaaagacga agaagccgag
     3061 gatcagcagg ccgaggaagc gcaaggggcg gaatctttta cgcatgaccc tatcgtgcgg
     3121 aacggcgtcg taaatgacct cttcgccgag gcccggaatg gcgtcttcgg gttggtctgt
     3181 catccggcgc cccccacgcg ttttaagctt ccgcctcgaa gatatacacg taaccgtcct
     3241 cgtcgccaat cagcaggcgg ttggcttcat tggagagggc cagggccgtg atcggcgcgc
     3301 ctttctcggc cttcacccag tcgacgccgg tcgattgcag ctcggccagc cagacgcgcc
     3361 cgtcctcggt cccggcggcc agcagcgtgt cctgcggcgc gcccgccacc agggagacga
     3421 gggtcgcttc ctgcgggttg atctcggagg cttcctcgcc catcgggccg gtcggtttca
     3481 ggaacggcca gacgaccgcg cccatcgcgc cggaggtggc gaacagcttg cccttggcga
     3541 agaagcccat ggacttgatc ttggccggat agccgcccat gcgcatatcc ttggaatcct
//
LOCUS       DASVLL010000584         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_38894, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000584 DASVLL010000000
VERSION     DASVLL010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38894"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VF402_09600"
     CDS             <1..245
                     /locus_tag="VF402_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800592.1"
                     /translation="NWRDKYLQQANASQNGGLYTAAYGQWDASLNYTLRPNIVLSFEA
                     LNLNKGHIVQYVRVPTDVVNYQELDSRYEFGIRYKF"
     gene            422..1159
                     /locus_tag="VF402_09605"
     CDS             422..1159
                     /locus_tag="VF402_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX7800593.1"
                     /translation="MGQHVLLNNVDHHDLRLITRHGAAFGDSVNQTLIFPSEYQDIQR
                     HYPIFFRQTENGGFKSVAILGFDRDENLFLDGDRWNAAYVPAMHRISPFLIGQPQAAP
                     GQVLPDDAEAMILVDPDHARISRADGEALFLSHGGNSPILTQVTETLRHVYAGSQVSD
                     RMFAAFFEAGLMAPVEINVQISEKQSFLLKDMATISREALQNLDAARLKALNEAGFLA
                     LAFFVVASLGNLSHMIALKTEHDTATA"
     gene            1253..>1664
                     /locus_tag="VF402_09610"
     CDS             1253..>1664
                     /locus_tag="VF402_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX7800594.1"
                     /translation="MVFKGLAADLPLVRAGLVSDEAAMQHLLPYYGGKPLLVFKGEPP
                     IRGRFGYTPEVDDFNFTSEHMTLDRLFDLLRAHRDDAESPSFYCGSTDIDTFLPGLPP
                     DDGLPLSGEMFMRNAVLKSIWLGNRTTACAHFDIS"
BASE COUNT          316 a          566 c          466 g          316 t
ORIGIN      
        1 acaactggcg cgacaagtac ctgcaacagg cgaacgcctc gcaaaacggc ggtctctaca
       61 ccgcggccta tggccagtgg gacgcctcgc tgaactatac gctgcgtccg aacatcgttc
      121 tgtcgttcga agcgctgaat ctcaacaagg gccacatcgt tcagtacgtc cgcgtgccaa
      181 ccgatgtcgt gaactatcag gaactggatt cgcgttacga attcggcatc cgctacaagt
      241 tctaagcccc tctcaggctt agacatggga gggtcgcggg caactgcggc cctcctcttt
      301 atttatcgga gggcggcgca aggatttacc tctcgcttcc ggccggtctc ccgcgcgtga
      361 cgcgattgcc ttttgagcgc cgtcgccgca ttatcttttc atgcgataaa gggcggatac
      421 cttgggacag cacgtcttac tcaacaatgt cgaccatcac gacctgcgcc tgatcacgcg
      481 ccacggcgcc gcgttcggcg acagcgtcaa tcagaccctg atcttcccgt ccgaatatca
      541 ggacatccaa cgccattatc cgatcttctt ccgccagacg gagaacggcg gcttcaaatc
      601 ggtcgccatt ctgggtttcg accgggacga aaacctgttt ctcgacggcg accgctggaa
      661 cgccgcctat gttcccgcca tgcaccgcat cagcccgttc ctgatcggcc agcctcaggc
      721 cgcgcccgga caggttctgc ccgacgacgc cgaggcgatg attctcgtcg atcccgacca
      781 cgcccgcatc agtcgcgccg acggcgaagc cctcttcctg tcgcacggcg gcaacagccc
      841 gatcctgacg caggtgaccg agaccttgcg ccacgtctat gccggttcgc aggtcagcga
      901 ccgcatgttc gccgccttct tcgaggcggg cctgatggcg cccgtcgaga tcaacgtcca
      961 gatcagcgaa aaacaatcct tcctgctgaa ggacatggcc accatcagcc gcgaagcgct
     1021 gcaaaacctc gacgccgcgc gcctgaaagc cctgaacgaa gccggtttcc tggcgctggc
     1081 cttcttcgtc gtggcgtcac tgggcaatct cagccatatg atcgccctga agaccgaaca
     1141 cgacacggcg acggcctgat ccatgaccgc gccggtgaga caagtcgcga caacccggat
     1201 cggagccctt gcggagatag attttcagcg gctcatcgat gagcagacgc cgatggtctt
     1261 caaggggctg gcggcggatc tgccgctggt cagggccggg ctggtctccg acgaggccgc
     1321 catgcagcac ctgctgccgt attacggcgg caagcccttg ctggtcttca aaggcgagcc
     1381 gccgatccgg ggacgtttcg gctacacgcc ggaggtcgac gatttcaact tcacctccga
     1441 acatatgacg ctggaccgcc tgttcgatct gctgcgcgcg caccgggacg acgccgaatc
     1501 cccgtccttc tattgcggct cgaccgacat cgacaccttc ctgcccggcc tgccgcctga
     1561 cgacggcctg ccgctgtccg gcgagatgtt catgcgcaac gcggtgctga aaagcatctg
     1621 gctgggcaat cgcaccacgg cctgcgccca tttcgacatt tcgc
//
LOCUS       DASVLL010000585         6698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_415588,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000585 DASVLL010000000
VERSION     DASVLL010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415588"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="VF402_09615"
     CDS             complement(<1..1651)
                     /locus_tag="VF402_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7800595.1"
                     /translation="MKKTVHTTRARVRLTSGTALGLVALFAFSTLAHAQTAETSNSTT
                     AGQTQSLSDAKKKAASEDSTLVIVTGVRASQRSSIDRKKHAKTAEDSIVAEDIGQFPD
                     KNVDEAISRIAGVSLDRGDNGEGQGFSIRGQGMETTLVDVDGMSTLNTNGGIAGGAGS
                     ADGGRGADLRELPADMVKSIDVIKGSTAAMTEGGLGGSVHIETRTGLDFKKPFFQLTV
                     DEQMNSISKQWTPEWSAVFARRFFGGRLGMTGNINYSETQTNADAQQPQTSGNAGPSR
                     NADFDQSPDKTFTYDPSIADPSATDGNFRVLNASGSPVYSSASPIDILTKSAAATSKA
                     DCYADFPALTSAQLAAVPSGNNVLGTVATAGVSQSNNSKAAQLEQANELQTCLNQWND
                     YAPSLVRYFSKRAFERRVAAQLRFDYKVNDDLTLYATGNIRNRTVDNRDDTLNLGSPA
                     YNQGNVCIQGVCNTASSQATPAATSNTLLTPRTVATGLGYGYYSNGIPGCATTVAGTG
                     TAQTTTGCGVESDITNAVVDDTHHLTSFTLNDANANIDAVHY"
     gene            complement(2079..3440)
                     /locus_tag="VF402_09620"
     CDS             complement(2079..3440)
                     /locus_tag="VF402_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEX7800596.1"
                     /translation="MNPSKRDFLTGAAVLTAAVAAPALAQTSSALPAGPPAGPKTTAA
                     GPVPKDWIDPDTGHRIVRLSSQDHSQSLYFNFPAYTPDEKWMVMNRADGISLIDMRTF
                     EEKNLYTGNLSAFQTGASKAVVYARPRSADKDNATQATNDIFAIDVATGKATKIAAVA
                     KGFVTSVNADDTLLVGSYAYKDVPLQPGPKVAGTDGGYNAVGPDGKPMSFAAAKEYRM
                     AQRLAADVPMDIFTIDIATGARKVLVHSTDWLNHMQFSPADPSLLLYCHEGPWHEVDR
                     IWTIRTDGTGNQLMHKRMMNMEIAGHEFFNWDGSKIFYDLQTPRGEDFWLANVDLKTG
                     KRVWYHMLRDEWSVHFNVSKDGTLFAGDGGDSEMVARAPDGKYIYLFRPEIIEDLGVS
                     APNAADLVRPGVLRSEKLVNMKDHDYRLEPNLRFSPDGKWIIFHSNMHGPVHTYAVAV
                     EKA"
     gene            3663..5684
                     /locus_tag="VF402_09625"
     CDS             3663..5684
                     /locus_tag="VF402_09625"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7800597.1"
                     /translation="MPNRLMIAVTASLMALTLGAGLAQAQDRARTPTLTIHADQVQAR
                     VSPDLYGLMTEEINFSYEGGLYGELIRNRSFKADPSAPAWWDAVGDAKLSLDTATPLN
                     AALDLSLKLDAGGASKARPAGIANGGYWGVPVRPRTTYRASFYAKGFKGPLKISLQSA
                     DGQKVFASADIKAAGGDWRRYEATLTTGDVAVTKAARFVLTTTQPGTVWLQQVSLFPP
                     TYNNRANGDRPDLMRKLADMRPAFLRMPGGNYLEGDTIPERFDWKKTLGDPAARPGHQ
                     SPWKYWSTDGMGLLEFLEWCEDLHMQPVLAVYAGYSLHQDRVAAGPDLEPYVQDALDE
                     IEYVTGDVTTKWGARRARDGHPAPFPLNYVEIGNEDWFDKENTYDARFTQFYDAIKAK
                     YPRLRLISTISPDRPADQHVKSRTPDLIDEHLYPDEEGAMEARAHEFDDLPRTGPKIF
                     VGEWATRVGAPTPNMAAALADAAWMTGMERNSDLVVMESYAPLLVNVSNPKGGRDPQS
                     SMQWPSDLIGYDALTSYGSPSYYAQVMFSNHHGDEVLKSEAADIPTWTWTPKIRTRNG
                     VQEPPRPPQQLPSLYYDVTRNSRDGTLYLKIVNRDDTAQSVHVVIDGATIAPDGEAVV
                     MSASSRDDTNTLSDPEKIKPAATPIRGLSADFTCDFPALSITVLKLKSR"
     gene            5755..>6698
                     /locus_tag="VF402_09630"
     CDS             5755..>6698
                     /locus_tag="VF402_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HEX7800598.1"
                     /translation="MWNWRHADWPNFRWDARKLVAAEAAFAEGAGVVIGASKHLDAAD
                     RQSLSVERLSHEAVDTSAIEGERLDRNSVQSSIQRQLGLAVAQRRATPAETGVADMMV
                     DLYRRLAEPLSEDMLLNWHRQLMNGRADLHDIGRYRTDPEPMRIVSGALHAPKVHYEA
                     PPAPQVAVEMARFLKGHARTAPDGATPLPAVTRAGLAHLWFESVHPFEDGNGRIGRAI
                     AEKALAQGLSTPVITGMAGTLLKHRQAYYAALEQASRTLDVTDWLLWFAGMAIEAQQR
                     TQAQIDFILAKTRLLARLHGRFNPRQEKALLRMFAEGP"
BASE COUNT         1192 a         2149 c         2149 g         1208 t
ORIGIN      
        1 gatagtggac cgcgtcgatg ttcgcattgg cgtcgttcaa cgtgaacgag gtcaggtggt
       61 gcgtgtcgtc gaccacggcg ttggtgatgt cgctttcgac gccgcaaccg gtcgtcgtct
      121 gcgccgtgcc ggttcccgcg acggtggtcg cgcagcccgg aatgccgttc gaataatagc
      181 cgtaacccag tcccgtcgcc acggtgcgcg gcgtcaggag cgtgttcgag gtcgccgccg
      241 gcgtggcctg ggacgaagcc gtgttgcaca cgccctgaat acagacattg ccctgattgt
      301 aagccgggct gcccaggttc agcgtgtcgt cgcggttgtc gacggtgcgg ttgcggatat
      361 tgccggtggc gtacagggtc aggtcgtcgt tgaccttata gtcgaagcgc agttgcgccg
      421 ccacgcgacg ctcgaacgcg cgcttggaga aatagcgcac cagcgagggc gcgtagtcgt
      481 tccactggtt caggcaggtt tgcagttcgt tggcctgctc aagctgcgcc gccttggagt
      541 tgttcgactg gctgaccccg gcggtggcca cggtgccgag gacgttgttg ccgctcggaa
      601 cggcggccag ttgcgcgctg gtcagcgccg ggaagtcggc atagcaatcc gccttcgagg
      661 tcgcggccgc cgacttggtc aggatgtcga tcggcgacgc cgacgaatac acgggcgagc
      721 cgctggcgtt cagcacgcgg aagttgccgt ccgtcgcgga cggatcggcg atcgacggat
      781 cgtaggtgaa ggtcttgtcc ggcgactggt cgaagtcggc gttgcgcgac ggaccggcgt
      841 tgcccgaggt ctgcggctgc tgcgcatccg cgttggtctg ggtttcggaa tagttgatgt
      901 tgccggtcat gccgaggcgg ccgccgaaga agcggcgggc gaagacggcg ctccattccg
      961 gcgtccactg cttcgagatc gagttcatct gctcgtcgac cgtcagctgg aagaacggct
     1021 ttttgaaatc gaggccggtg cgggtttcga tgtggacgct gccgcccaga ccgccttcgg
     1081 tcatggccgc ggtcgagccc ttgatcacgt cgatgctctt gaccatgtcg gccggcagtt
     1141 cgcgcaggtc ggcgccgcgg ccgccgtcgg cgctgcccgc gccgcccgcg atgccgccat
     1201 tggtgttcag cgtgctcatg ccgtcgacgt cgaccagcgt cgtctccatg ccctggccgc
     1261 ggatcgagaa gccctggcct tcgccgttat cgccgcggtc cagcgacacg ccggcgatgc
     1321 gcgaaatggc ttcgtcgacg ttcttgtccg ggaactggcc gatatcttcg gcgacgatcg
     1381 agtcttccgc cgtcttggcg tgcttcttgc ggtcgatcga cgaacgctgc gaggcgcgca
     1441 cgccggtcac gatgaccagg gtggaatcct cggacgccgc cttcttcttg gcgtccgaca
     1501 gggactgcgt ctggccggcg gtcgtggaat tgctggtttc cgccgtctgg gcatgggcca
     1561 gcgtgctgaa cgcgaacaaa gcgacaaggc ccagggcggt gccgctggtc aggcgcacgc
     1621 gcgcacgggt ggtgtgaacc gtcttcttca cctttttcct cctggcgcgg ttggccgcgc
     1681 ttttgtgaat cgattgttgc gccggacgaa cccacgccga gcggtacgcg cggccggcgc
     1741 gggttttccc gtcgctcgac cgcaattgct ctttgtgtcc tgttcgggac gcaatgtctt
     1801 agtgccgaca tctttgttga ttgataatga caggtcaaga caatttatgc gcgcttgtgc
     1861 aatatatgtt agacaattga tcacaaaccg tcccaagcgt aacaaaaacg ccacagatca
     1921 tgaccggaag cgttaaacgc ccgtcatttt ctgtcgtgtt cgcaagagat tcgccgccgg
     1981 acgtgtccgc gccgccccgt tcgcgcgcgt catgcgcaca acgaaaacgc gtccggtctg
     2041 accagaccgg acgcgcgaat tgatcgaatt tgatcgaatc aggctttttc caccgccacg
     2101 gcgtaggtgt ggacggggcc gtgcatgttg gaatggaaga tgatccactt gccgtccggc
     2161 gaaaagcgca ggttcggctc cagccggtag tcgtggtcct tcatattgac cagcttttcc
     2221 gaccgcagca cgccgggccg caccagatcg gcggcgttgg gcgcgctgac gccgagatcc
     2281 tcgatgatct ccgggcggaa cagatagatg tatttgccgt ccggcgcgcg cgcgaccatc
     2341 tccgaatcgc cgccgtcgcc cgcgaacagc gtcccgtcct tcgagacgtt gaaatgcacc
     2401 gaccattcgt cgcgcagcat gtgataccag acgcgcttgc cggtcttcag atcgacattg
     2461 gccagccaga aatcctcgcc gcgcggcgtt tgcaggtcgt agaagatttt cgagccgtcc
     2521 cagttgaaga actcatgccc ggcgatctcc atgttcatca tgcgtttgtg catgagctgg
     2581 ttgcccgtgc cgtcggtgcg gatggtccag atgcggtcga cctcgtgcca cggcccttcg
     2641 tggcagtaca gcagcagcga cggatcggcg ggcgagaact gcatgtggtt gagccagtcg
     2701 gtcgaatgga ccagcacctt gcgcgcgccc gtggcgatgt cgatggtgaa gatgtccatc
     2761 ggcacgtcgg cggccaggcg ctgcgccatg cggtattcct tggcggcggc gaagctcatc
     2821 ggcttgccgt ccgggccgac cgcgttatag ccgccgtccg tgcccgcgac cttcggcccc
     2881 ggttgcagcg gcacgtcctt ataagcatag gagccgacca gcagcgtgtc gtcggcgttg
     2941 accgaggtga cgaagccctt ggcgacggcg gcgatcttcg tcgccttgcc cgtggcgacg
     3001 tcgatggcga agatgtcgtt ggtcgcctgg gtcgcgttgt ccttgtccgc cgaacgcggg
     3061 cgggcataga ccacggcctt cgacgcgccg gtctggaagg ccgacagatt gccggtatag
     3121 aggttcttct cctcgaaggt gcgcatgtcg atcaggctga tcccgtcggc gcggttcatc
     3181 accatccact tctcgtccgg cgtataggcc gggaagttga aatagagcga ttgcgaatgg
     3241 tcctggctgg acagccgcac gatgcggtgg ccggtgtcgg gatcgatcca gtccttcgga
     3301 accggcccgg cggcggtcgt tttcggaccg gcgggcggcc cggcgggcag cgcagaagac
     3361 gtctgggcca aggccggggc ggcgaccgcc gcggtcagaa cggcggcgcc ggtcagaaag
     3421 tcgcgcttgc tggggttcat cgtctcatct cctgcatgtc cggcattttg aacgcagcat
     3481 agcggcggaa acgtcgctcg gaaatgaatt tatttgaatg attatgattg aattgcggat
     3541 ttgtcatttg tcctacatat tgcgcggcgt cgtgctgtaa gaaccgtgct gctcccatca
     3601 aaaatcaggc tcaaaataaa gcccaaaaca aagcccaaaa caaagccaag gaaacaaaga
     3661 caatgcccaa ccgtctcatg atcgccgtta cggcctccct catggcgctg acgctcggcg
     3721 ccggtctcgc tcaggctcag gatcgggcgc ggacgccgac cctgaccatc cacgccgatc
     3781 aggttcaggc cagggtcagc cccgacctct acgggctgat gaccgaggag atcaacttct
     3841 cctacgaagg cggcctgtac ggcgaactga tccgcaaccg cagcttcaag gccgatccgt
     3901 ccgccccggc gtggtgggac gcggtcgggg acgccaagct cagcctcgat acggccacgc
     3961 cgctcaacgc cgcgctcgat ctgagcctga aactggacgc aggcggcgcc tcaaaggccc
     4021 gtccggcggg gatcgccaac ggcggctact ggggcgtgcc ggtccgcccg cgcacgacct
     4081 atcgcgccag cttctacgcc aaaggcttca aaggcccgtt gaagatttcc ctgcaaagcg
     4141 cggacggaca aaaggttttc gccagcgccg acatcaaggc ggcgggcggc gactggcggc
     4201 gttacgaagc gacgctgacc accggcgacg tcgcggtcac caaggccgcg cgcttcgtcc
     4261 tgacgacgac gcagcccgga accgtctggc tgcaacaggt ctcgctgttc ccgccgacct
     4321 acaataaccg cgccaatggc gaccgtcccg acctgatgcg gaagctggcc gacatgaggc
     4381 ccgccttcct gcgcatgccg ggcggcaact atctcgaagg cgacaccatc ccggagcgct
     4441 tcgactggaa aaagacgctg ggcgatccgg cggcgcgtcc gggccatcaa agcccgtgga
     4501 agtactggtc caccgacggc atgggcctgc tggaattcct cgaatggtgc gaggatctgc
     4561 acatgcagcc ggtgctggcc gtctatgccg gttattcgct gcatcaggac cgcgtggcgg
     4621 cggggccgga cctcgaaccc tatgtgcagg acgcgctgga cgaaatcgaa tacgtcaccg
     4681 gcgacgtgac gaccaaatgg ggcgcccggc gcgccaggga cggccatccg gcgcccttcc
     4741 cgcttaacta tgtcgagatc ggcaacgagg actggttcga caaggaaaac acctacgatg
     4801 cgcgcttcac gcagttttac gatgcgatca aggcgaaata tccgcgcctg cgactgatct
     4861 cgaccatctc tcccgaccgc cccgccgatc agcacgtcaa aagtcgtacg cccgacctga
     4921 tcgacgaaca cctctacccc gacgaggaag gcgcgatgga ggcgcgcgcg cacgaattcg
     4981 acgacctgcc gcgcaccggg cccaaaatct tcgtcggcga atgggcgacg cgcgtcggcg
     5041 ccccgacgcc caacatggcc gccgccctgg ccgacgcggc gtggatgacc ggcatggagc
     5101 gcaattccga cctcgtggtc atggaatcct acgcgccgct gctggtcaat gtctcgaacc
     5161 cgaaaggcgg acgcgatccg caaagctcga tgcagtggcc cagcgacctg atcggctatg
     5221 acgccctgac ctcgtacggc tcgccgtcct attacgctca ggtcatgttc tcgaaccacc
     5281 acggcgacga ggtgctgaaa agcgaggcgg cggacatccc gacctggacc tggacgccga
     5341 aaatccgtac ccgcaacggc gtgcaggaac cgcccaggcc gccgcagcaa ctgccgtccc
     5401 tctattacga cgtcacgcgc aacagccgcg acgggacgct gtacctgaag atcgtcaacc
     5461 gcgacgacac ggcgcaaagc gtgcatgtcg tcatcgacgg cgcgaccatc gccccggacg
     5521 gcgaggccgt ggtcatgtcg gcgtcgagcc gcgacgatac gaacaccctg agcgaccctg
     5581 aaaagatcaa gcccgccgcc acgcccatcc gcggcctgtc cgccgacttc acctgtgact
     5641 ttccggcgct ctcgatcacc gtgctgaagc tgaaaagccg gtgatcgata gggcatggat
     5701 ttataaatca taatatggca tgtttatgat cgataaaagg agtccctatc gatcatgtgg
     5761 aactggcggc atgcggattg gccgaacttc cgctgggacg cgcgcaagct ggttgcggcg
     5821 gaagccgcct tcgccgaagg cgcgggcgtc gttatcggag cgtcgaaaca tctggacgcc
     5881 gccgaccgcc agagcctgag cgtcgagcgt ctcagccatg aggccgtcga cacttccgcc
     5941 atcgaaggcg aacggctgga ccgcaacagc gtgcaatcct cgattcagcg tcagctcggt
     6001 ctcgccgtcg cccagcgccg cgccacgccc gccgaaaccg gcgtcgcgga catgatggtc
     6061 gatctctatc gccgcctagc cgaaccgctg agcgaggaca tgctgttgaa ctggcatcgc
     6121 cagttgatga acggccgcgc cgatctgcac gacataggcc gctatcgcac cgacccggag
     6181 ccgatgcgga tcgtctccgg cgctctgcat gcgccgaaag tgcattacga agccccgccc
     6241 gccccgcagg ttgccgtgga gatggcgcgc ttcctgaagg ggcacgcccg caccgcgccc
     6301 gacggcgcga cgcccctgcc cgcggttacg cgcgccggtc ttgcgcatct gtggttcgag
     6361 agcgtccacc cgttcgagga cggcaacggg cgcatcggtc gcgccatcgc cgaaaaggcg
     6421 ctcgcgcagg gcctgtccac accggtcatt accggcatgg ccggaacctt gctgaaacac
     6481 cgccaggcct attacgccgc gctggaacag gccagccgga cgctggacgt caccgactgg
     6541 ctgttgtggt tcgcgggcat ggccattgaa gcgcagcaac gcacgcaggc gcagatcgac
     6601 ttcatcctgg ccaagacgcg gttgctggcg cgcctgcatg gccggttcaa tccccgtcag
     6661 gaaaaagcct tgctgcgcat gttcgccgaa ggaccgga
//
LOCUS       DASVLL010000586         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_93854, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000586 DASVLL010000000
VERSION     DASVLL010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93854"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1231
                     /locus_tag="VF402_09635"
     CDS             209..1231
                     /locus_tag="VF402_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX7800599.1"
                     /translation="MNAAVPASDGSGKAAGGEPLVLIPDLAEPPALHRTAQRLSFAGE
                     AFATTWRVRLCADPARFRGPDALRAACAARLSRIDAEMSPYRKDSDLTRFNRAAAGEF
                     VALPALTMQVLQGALEIAALTGGAFDPTVLAAVELWGFGARAVPEGLPDPVDVAALPR
                     PRWRDLAPGQGGLIKPHDAGLDLCGIAKGFAVDELLRLCRAEPGVTAALVEVGGELKG
                     WGVQPDGQPWWVGIEDTDARAALCGWAVATSGDGRRAFRHAGRLYSHTIDAATGAPAS
                     SGVASATVFDPACWRADALATALIVMGEARALRFATEHDLPCLLRVRAGGEVRTRLSP
                     RLESWA"
     gene            1231..2529
                     /locus_tag="VF402_09640"
     CDS             1231..2529
                     /locus_tag="VF402_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH cytochrome P450 oxidoreductase family
                     protein"
                     /protein_id="HEX7800600.1"
                     /translation="MFDVTHDTARLWWSGAALLAFGGLSWARLRRAPRAKAPPCAVRV
                     LYASQTGQAEAIARRTHARLIAGGIDAGLASLADIDAAALLSARTLLVVASTTGEGDA
                     PDDGRAFESVLAARPGLSGRDFAVLALGERKYAAFCAFGLRVHDGLAACGATALGPCL
                     TADDLDAATLRRWDDLADTLGAGAAPVEPEAPPWTLSARERLNPDGGAPLYRLRLVPP
                     VTCEWQAGDLAEVGTKDGHRRDYSIATLPGEGHVELYVREAVGPDGLPGKGSGLLLHD
                     TPVGGQIRLRLKSHKAFHAPAGDGPLLLIGAGSGLAGLRAHILASRRRRPWLIYGERH
                     PVRDGALVAEAQDWLAARALSRLSLAFSQAGDGTKTYVQDVLNAEGARVYDHLIHDGA
                     VLVCGGLDMGRAVERTLRGLMGDGWIDDALASGRYRRDLY"
     gene            2541..>3374
                     /locus_tag="VF402_09645"
     CDS             2541..>3374
                     /locus_tag="VF402_09645"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEX7800601.1"
                     /translation="MKRLLHPALIALAAAALLPLPAQAHRSWLMPSQAFVDGKGQTVT
                     FDGGVSENLFQTDAFALKLDGLTITAPDGSALQPQDVTTAKQRSSFDLSLPQEGTYRV
                     AVAGDTVMASYLLNGELQRFRGAAADMAARIPAGATDVRVMHMLSRYETWVTVGAATP
                     DALKPAGHGLEIVPLSDPSAYVAGEAASFRAYLDGKPLAGLTVQVIPGGVRYRGDLKE
                     TDATTDARGEFSVTWPIGQMYWIGASYPPRPQGGEDGKAPGGQNGGAHPAAQAQGGEG
                     GG"
BASE COUNT          444 a         1210 c         1262 g          458 t
ORIGIN      
        1 cagctggtgg tcgaggcggc gcgcgaaaac ggcggccaca gcctcgtcag gcttcccttc
       61 aactggagcg gcaaggcgtt cagcgccacg ggccagacca cgggcgagct cggcgccgtg
      121 acggtgagct acaagccgta accttatgac gcttccggcc tccccctctc accgcttcgc
      181 ggcttccccc ttcgccgggc gggacggcgt gaacgccgcc gtccccgcca gcgacggaag
      241 cgggaaagcg gcgggcgggg agccgctcgt cctgatcccg gacctcgccg agccgcccgc
      301 cctccaccgg acggcgcagc ggctgagctt cgcgggcgag gcgttcgcga cgacgtggcg
      361 ggtgcggctg tgcgcggatc cggcgcggtt tcgcggcccg gatgccctgc gcgccgcctg
      421 cgcggccaga ctgtcgcgca tcgacgccga gatgagcccc tatcgcaagg actccgacct
      481 gacgcgcttc aaccgcgcgg cggcgggcga attcgtggcc ctgcccgcgc tgacgatgca
      541 ggtgctgcaa ggcgcgctgg agatcgccgc gctgaccggc ggcgccttcg atccgacggt
      601 gctggccgcc gtcgagctgt ggggcttcgg cgcgcgcgcc gtgccggagg gattgcccga
      661 ccccgtcgat gtcgccgccc tgccgcgccc gcgctggcgc gacctggcgc cgggtcaggg
      721 cggactgatc aagccgcatg acgcggggct ggacctgtgc ggcatagcca agggcttcgc
      781 ggtcgacgaa ctgctgaggc tctgccgcgc cgagccgggc gtgacggcgg cgctggtcga
      841 ggtcggcggc gaactcaagg gctggggcgt gcagcccgac ggccagccgt ggtgggtcgg
      901 gatcgaggat acggacgcgc gcgccgcgct gtgcggctgg gcggtggcga cctccggcga
      961 cggacggcgc gccttccgcc acgccgggcg gctctattcg cacaccatcg acgcggcgac
     1021 cggcgcgccc gcatcgtccg gcgtcgcctc ggcgacggtg ttcgatccgg cctgctggcg
     1081 cgccgacgcg ctggccaccg ccctaatcgt catgggcgag gcacgcgcct tgcgatttgc
     1141 aaccgaacac gacctgccct gcctgctcag ggtgcgcgcg ggcggcgagg tccgcacgcg
     1201 gctcagcccg cgcctcgaaa gctgggcctg atgttcgatg tgacgcacga tacggcgcgg
     1261 ctttggtggt cgggcgcggc gctgctggcc ttcggcgggc tgagctgggc gcggctgaga
     1321 cgcgcgccgc gcgccaaggc gccgccgtgc gccgtgcgcg tgctctacgc ctcgcagacc
     1381 ggtcaggccg aggccatcgc gcgccggacc catgcgcgcc tgatcgcggg cgggatcgac
     1441 gccgggctgg cctccctggc cgacatcgac gcggcggcgc tgctcagcgc gcggaccctg
     1501 ctggtcgtcg cctcgaccac cggcgaaggc gacgcgcccg acgacggccg cgcgtttgaa
     1561 agcgtgttgg cggcgcgccc cggcctgagc gggcgggact tcgccgtgct ggcgctgggc
     1621 gagcgcaaat acgccgcctt ctgcgccttc ggcctgcgcg tgcatgacgg actggcggcg
     1681 tgcggcgcga cggcgctcgg cccgtgcctg accgccgacg atctcgatgc ggcgaccctg
     1741 cggcggtggg acgatctggc cgatacgctg ggcgccggag ccgcgccggt ggagccggag
     1801 gcgccgccgt ggacgctgag cgcgcgcgaa cggctgaacc cggacggcgg cgcgccgctc
     1861 taccgcctgc gcctcgtccc gcccgtgact tgcgaatggc aagcgggcga tctggccgag
     1921 gtcgggacga aggacggcca ccgccgcgac tattccatcg ccaccctgcc cggcgagggc
     1981 catgtcgagc tttacgtgcg cgaagccgtc ggccccgatg gcttgccggg caagggatcg
     2041 ggcctgctgc tgcatgacac gcccgtgggc gggcaaattc gcttgcgcct caaaagtcac
     2101 aaggctttcc acgccccggc gggcgacggg ccgctgctgc tgatcggcgc cggatcgggg
     2161 ctggcgggcc tgcgcgcgca catcctcgcc tcgcggcggc gcagaccgtg gctgatctat
     2221 ggcgagcgcc atccggtccg cgacggcgcg ctggtcgccg aggcgcagga ctggctggcc
     2281 gcgcgcgcgc tgtcacggct gagcctcgcc ttctcgcaag ccggcgacgg gacgaaaacc
     2341 tatgtccagg acgtgctgaa cgccgagggc gcgcgcgtct atgaccacct gatccatgac
     2401 ggcgccgtgc tggtgtgcgg cggcctcgac atgggccgcg ccgtcgaaag gacgctgcgc
     2461 ggcctgatgg gcgacggctg gatcgacgac gcgctcgcca gcggccgcta tcgccgcgat
     2521 ctttattaac ggagacgatc atgaaacgcc ttctgcaccc cgctctcatc gcgctggccg
     2581 ccgccgcgct cctgcccctg ccggcgcagg cgcaccgcag ttggctgatg ccgtcgcaag
     2641 ccttcgtcga cggcaagggc cagaccgtca ccttcgacgg cggcgtgtcg gaaaacctgt
     2701 tccagaccga cgccttcgcg ctgaagctgg acgggctgac gatcacggcg ccggacggct
     2761 cggcgctgca accgcaggac gtgacgaccg cgaagcagcg ttcgtccttc gacctgagcc
     2821 tgccgcagga aggcacgtat cgcgtcgccg tcgcgggcga tacggtgatg gccagctacc
     2881 tgctgaacgg cgaattgcag cgcttccgcg gcgcggccgc cgacatggcc gcccggattc
     2941 cggcgggcgc gacggacgtg cgcgtcatgc acatgctcag ccgctacgag acgtgggtga
     3001 cggtcggcgc ggcgacgccg gacgcgctga aacccgcagg tcacgggctg gagatcgtcc
     3061 cgctcagcga tccgtcggcc tatgtggcgg gcgaggcggc gtcgttccgc gcttatctcg
     3121 acggcaagcc gctggcgggc ctgacggtgc aggtcatccc cggcggcgtg cgctatcgcg
     3181 gcgacctgaa ggaaaccgac gccacgaccg acgccagggg ggaattctcg gtcacctggc
     3241 cgatcggcca gatgtactgg atcggcgcca gctatccgcc gcgcccgcaa ggcggcgagg
     3301 acggcaaggc ccccggcggc cagaacggcg gcgcgcatcc ggcggctcag gcgcagggcg
     3361 gcgaaggcgg cggt
//
LOCUS       DASVLL010000587         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_309602,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000587 DASVLL010000000
VERSION     DASVLL010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309602"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..>1612)
                     /locus_tag="VF402_09650"
     CDS             complement(94..>1612)
                     /locus_tag="VF402_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucuronidase"
                     /protein_id="HEX7800602.1"
                     /translation="SVGINGAVIDNVNASPLILSDRYIAKAAAVADMLRPWGVKVYLA
                     VNFASPKALGGLPTADPLDPRVIAWWQARTDAVYKAIPDFGGYLVKASSEGQPGPGDF
                     GRSHAEGANMLARAVKPHGGIVMWRAFVYASDNPEDRFKQAYDEFKPLDGQFDDNVVI
                     QIKNGPIDFQPREPFSPLFGALTKTNEMMEVEITKEYLGQATHLVYLAPMWREALQSD
                     TYRQGPGTTVAKVLEGRVYGNRLTAMAGVANIGADRNWTGSDFDQANWYAYGRLAWDP
                     DADPKAIATDWTKMTFSDDPAFVKPVVDMMMGSHEAVVDYMTPLGLAHQMATGHHYGP
                     GPWVCDLGRPEWNPCYYARADKDGIGFDRTATGSDALAQYAPEAAAKWRDARTMDPRF
                     LLWFHHVPWDFVSADGKPLWPDMIAHYDAGVDYVHAMQKTWAGLAPYVDDRRFTAVGE
                     NLGIQAREAKWWRDASIAYFQSRNGLALPAGETPPDKSLGYYESLQFPNAPGQGK"
BASE COUNT          269 a          548 c          512 g          283 t
ORIGIN      
        1 cgtagccatg acgcgtctcc ttccgacagg gcgtgaatac accgccagca taggaaaacc
       61 cggcataaga atgcgatgcg aagctgaccc cgcctacttt ccttgccccg gcgcgttcgg
      121 aaattgcagc gactcgtaat agccgaggct cttgtctggc ggcgtctcgc ccgcgggcag
      181 ggcaaggccg ttcctgctct ggaagtaagc gatggaggcg tcgcgccacc acttcgcctc
      241 gcgcgcctga ataccgaggt tctcgccgac cgccgtaaag cgccggtcat cgacataggg
      301 cgcaagaccg gcccaggtct tttgcatggc gtggacataa tcgacccccg cgtcgtaatg
      361 cgcgatcatg tcgggccaca acggcttgcc gtcggcggag acgaaatccc acggcacgtg
      421 atggaaccag agcaaaaagc gcgggtccat cgtcctggcg tcgcgccact tcgccgccgc
      481 ttccggcgcg tattgcgcca gcgcgtccga tcccgtcgcc gtgcggtcaa agccgatccc
      541 gtccttgtcc gccctggcgt agtagcacgg attccactcc gggcggccca gatcgcacac
      601 ccacggcccc ggcccgtaat ggtggccggt cgccatctgg tgcgcgaggc ccagcggcgt
      661 catgtaatcg accacggcct catgcgagcc catcatcatg tccacgaccg gcttcacgaa
      721 ggccggatcg tcggagaagg tcatcttcgt ccagtccgtc gcgatcgcct tcgggtcggc
      781 gtcaggatcc caggccagcc gcccataggc gtaccagttg gcctgatcga agtccgagcc
      841 ggtccagttg cggtcggcgc cgatattggc caccccggcc atcgcggtca gccggttgcc
      901 gtaaaccctg ccttccagca ccttcgcgac cgtggttccc ggcccctgcc ggtaggtgtc
      961 cgattgcagc gcctcccgcc acatcggggc cagatagacg aggtgcgtgg cctgaccgag
     1021 atattccttg gtgatctcga cctccatcat ctcgttggtt ttcgtcaacg cgccgaacag
     1081 gggcgagaac ggctcgcgcg gctggaaatc gatggggccg ttcttgatct ggatgacgac
     1141 attgtcgtcg aactggccgt ccagcggctt gaactcgtca taggcctgct tgaagcggtc
     1201 ctccgggttg tcgctggcgt aaacgaaggc gcgccacatc acgattccgc cgtgcggctt
     1261 gaccgcgcgc gccagcatgt tggcgccctc ggcatggctg cggccgaaat cgccggggcc
     1321 gggctggccc tcgctcgacg ccttgacgag gtagccgccg aaatcgggga tcgccttata
     1381 gacggcgtcg gtgcgcgcct gccaccacgc gatgacgcgc gggtcgagcg ggtcggcggt
     1441 gggcaggccg cccagcgcct tgggcgaggc gaaattgacc gccagataga ccttcacgcc
     1501 ccacggacgc agcatgtcgg ccaccgccgc cgccttggcg atatagcggt cgctcaggat
     1561 cagcggcgag gcgttgacat tgtcgatcac cgcgccgttg atcccgacgg ag
//
LOCUS       DASVLL010000588         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_525230,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000588 DASVLL010000000
VERSION     DASVLL010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525230"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF402_09655"
     CDS             complement(<1..481)
                     /locus_tag="VF402_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HEX7800603.1"
                     /translation="MAGVNPDLPFHPLNIAVLTISDTRNEETDTSGALLVGRLTSAGH
                     QLTGRAIAPDDVDAIRARLRMWIDDPHVDVVLTTGGTGFAPRDVTPEAVLPLLRRPMD
                     GFAVVFHNASLGTVGVSTLQSRALAGQAEDTFIFCLPGSTGACRDGWDLVLAQELDSR
                     "
     gene            complement(486..1154)
                     /gene="ric"
                     /locus_tag="VF402_09660"
     CDS             complement(486..1154)
                     /gene="ric"
                     /locus_tag="VF402_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEX7800604.1"
                     /translation="MMFTQDQLVRDIAVQLPGATAVFRRHKIDFCCKGGVPLAEAAQG
                     RGVDLAALLDALNGLQPAAREAPDATTELIPYILARYHEVHRQEFPEAIRMARRVEAV
                     HSDSPDCPHGLGDLLAIMAHELDEHQMKEETMLFPAMMNDAALDLNFPIQNMLMDHDD
                     LGEQLESMARLTAGFEPPPGACTTWRALYAACRKLDADLREHLHLENNILFPRFVTRP
                     EARL"
     gene            1332..1823
                     /locus_tag="VF402_09665"
     CDS             1332..1823
                     /locus_tag="VF402_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800605.1"
                     /translation="MKFLRRTSPRQALLAFSVFPAELALTRAVRTLRRRRPDIFARLG
                     RYQEAVYVLAPAGWPLAFRLTPGPERAGVRLVDAGRPGPFTVKITGGFRRLIGLFDGT
                     CDADSSFFSRAIVIEGATDAALALHNSLEAAALRPSDLLGLTGLPGEAFNRLLRPLRD
                     RHA"
     gene            1867..2832
                     /locus_tag="VF402_09670"
     CDS             1867..2832
                     /locus_tag="VF402_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX7800606.1"
                     /translation="MLRAAVEAGADSVYCGLSDETNARNFPGLNLLASELSEAVAWTR
                     ARGARLLLAVNTFATAGKVEAWRRAVDVAARAGVHAIIAADIGVLAYARAHWPDLRLH
                     LSVQAAAGTPQAIAFYARTYNVARVVLPRVLSLEEIERLTRSVEVEVEAFVYGGLCVM
                     AEGRCALSSYATEGSPNLTGVCSPPEAVAYTEAQASLTARLGDFAIDRHPAGEAAGYP
                     TLCKGRFLVDGDSSHLFEDPVSLNAMDLLDGFRRIGVTAVKVEGRQRGKAYVARVASA
                     FRRAIDAQAAGKTAASFAHTLADLALADLAEGGRQTSGAYRKTWR"
BASE COUNT          437 a          931 c          990 g          494 t
ORIGIN      
        1 accggctgtc cagttcctgc gccagcacca gatcccatcc gtcgcggcag gccccggtcg
       61 agcccggcag gcagaaaatg aaggtgtctt cggcctgacc ggccagcgcc cgcgattgca
      121 gtgtgctgac gccgacggtt cccagactgg cgttatgaaa gacgacggcg aaaccgtcca
      181 tcggccgtcg caagagcggc agcaccgctt cgggcgtgac gtcgcgcggc gcgaaaccgg
      241 tgccgcccgt ggtcaggacg acatcgacgt gcggatcgtc gatccacatc cgaagccggg
      301 cccggatggc gtcgacgtcg tccggcgcga tcgcgcggcc ggtcagttgg tgaccggcgg
      361 aggtcagccg cccgaccagc agggcgccgg acgtgtccgt ttcctcattg cgggtgtccg
      421 agatcgtcag caccgcgatg ttgagcggat ggaagggcag atcgggattg acgccggcca
      481 tgcgttcaga gcctcgcttc ggggcgcgtc acgaagcgcg ggaacaggat attgttttcc
      541 agatgcagat gctcgcgcag gtcggcgtcg agtttccggc aggcggcgta gagcgcgcgc
      601 caggtcgtgc aggcgcccgg cggcggttcg aaaccggcgg tgagccgagc catgctttcc
      661 agttgctccc ccagatcgtc gtgatccatc agcatgttct gtatgggaaa attcaggtcg
      721 agcgccgcgt cgttcatcat ggccggaaag agcatggtct cctccttcat ctgatgctcg
      781 tccagttcgt gagccatgat ggccagcagg tcgccgagcc cgtgcggaca gtcggggctg
      841 tcgctgtgga ccgcttcgac gcggcgcgcc atccggatgg cttcggggaa ttcctggcgg
      901 tggacctcgt gatagcgcgc gagaatatag gggatcagtt cggtcgtcgc gtcgggcgct
      961 tcccgcgcgg cgggctgcag gccgttcagg gcgtcgagca gcgcggcgag gtcgacgccc
     1021 cggccttgcg cggcttcggc cagcggcacg ccgcccttgc agcagaaatc gatcttgtgc
     1081 cggcggaaaa cggcggtggc gcccggcagt tgaacggcga tatcgcgcac cagttggtcc
     1141 tgagtaaaca tcatctgaac tccttacagg ccagacctac agcgcaaacg ccgcccgcgc
     1201 attcaaaggc gtcaaccacc cggctgaggg ttttcgcccg cgcgcttgat aaacttcgtt
     1261 gccgtcgcgc gcgccagggc ctctttaagc caaagcctct ttaagaaccg gctgacagca
     1321 tcggagcgaa catgaaattc ctgcgccgga cctcgccgcg gcaagcgttg ctcgcctttt
     1381 cggtctttcc ggcggaactg gccctgacga gggccgtgcg gacgttgcgg cggcggcgcc
     1441 cggatatttt cgcgcgcctt ggccggtatc aggaggccgt ttacgttctg gcgcccgccg
     1501 gatggccgct ggcgttccgc ctgacgccgg ggccggaacg cgccggggtc cggctggtcg
     1561 acgccgggcg gcccggtcct tttacggtga agatcacggg cgggtttcgc cgcctgatcg
     1621 gccttttcga cggaacctgc gacgcggact ccagcttttt ctcccgcgcc atcgtcatcg
     1681 aaggcgcgac cgacgcggcg ctggcgctgc ataacagcct tgaggcggcg gcgcttcggc
     1741 ccagcgacct gctggggctg acgggcctgc ccggcgaggc tttcaatcgc ctgctccggc
     1801 cgctgcgtga ccgtcatgcc tgagccggga tcgctcgaac tggtctgccc cgcgggctcg
     1861 cttgccatgc tccgcgccgc cgtcgaagcg ggcgcggaca gcgtctattg cggcctgagc
     1921 gacgagacga acgcgcgcaa ctttccgggg ctgaacctgc tggcgtccga gctgtccgag
     1981 gccgtcgcct ggacgcgggc ccgcggcgcg cgcctgttgc tggccgtcaa caccttcgcg
     2041 accgccggta aggtcgaggc ctggcggcgc gcggtcgacg tcgcggccag ggcgggcgtt
     2101 cacgccatca tcgccgccga tatcggggtt ctggcctatg cccgcgcgca ctggccggat
     2161 ctcaggctgc acctgtccgt acaggccgcc gccggcacgc cgcaggccat cgccttctac
     2221 gccaggacct ataatgtcgc gcgggtcgtg ctgccccgag tcctgagcct tgaggaaatc
     2281 gagcgtctga cgcgatcggt cgaggtggag gtcgaagcct tcgtctatgg cggattgtgc
     2341 gtcatggcgg agggccggtg cgccctgtcc tcctatgcga cggagggatc tccgaacctt
     2401 acgggcgtgt gttctccgcc cgaggcggtc gcctatacgg aggcacaggc gtcgctgacc
     2461 gcgcggctgg gcgatttcgc catcgaccgg catccggcgg gcgaagccgc cggctatccg
     2521 accctgtgca agggccggtt tctcgtcgat ggcgacagct cgcacctgtt cgaagatccc
     2581 gtcagcctca acgccatgga tttgctggac ggcttccggc ggatcggcgt cacggcggtc
     2641 aaggtcgagg gccgccagag aggaaaagcc tatgtcgcgc gcgtggccag cgcgtttcgg
     2701 cgcgccatcg acgctcaggc cgccggaaag acggcggcgt ccttcgctca tacattggcc
     2761 gatctggcat tagccgatct ggccgagggc ggacggcaaa ccagcggcgc ctacagaaag
     2821 acatggaggt gaagacatgg aggtgaaggc at
//
LOCUS       DASVLL010000589         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_201199,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000589 DASVLL010000000
VERSION     DASVLL010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201199"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF402_09675"
     CDS             complement(<1..212)
                     /locus_tag="VF402_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX7800607.1"
                     /translation="MPQNARGRPPLFVSLPIFWQVLSLSLTVLVLALGINTLLVLKAP
                     SPPPSGYTLNEVATALRRGEAPLQNG"
     gene            complement(229..960)
                     /locus_tag="VF402_09680"
     CDS             complement(229..960)
                     /locus_tag="VF402_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800608.1"
                     /translation="MSLTSPAAALDAPTRALIVDDDPDLRELISQFLSNNGFVVHTAS
                     QARDMDKLLARGDIDVIVLDYMMPGEDGLSVCKRLNEAGGPPVIMLSARGEEIDRIVG
                     LELGADDYMAKPFHPRELLARIRAVLRRREARSGGWDAKSPITHFFGWQLDNIKRTLL
                     RPDGVQVALSNAEFELLRVFLDRPGRALSRDQILDYLHGPNADSFDRAIDVQISRLRR
                     KLSDDHAEDIIRTIRGIGYMFKPLK"
     gene            1168..1632
                     /locus_tag="VF402_09685"
     CDS             1168..1632
                     /locus_tag="VF402_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX7800609.1"
                     /translation="MLRILVVTGALALGSSLAARAQEPPPRPDFAALDANHDGRLDLS
                     EFTAPMQARAKAVFDRMDAKHDGVVTESEFNAFRPENGPPRDGPPRMGPLRMGPPPDF
                     RSLDANGDGKINFAEFSAREKARFDRLDANHDGVLEPAELSPPKAAGGAVRP"
     gene            complement(1813..>3278)
                     /locus_tag="VF402_09690"
     CDS             complement(1813..>3278)
                     /locus_tag="VF402_09690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX7800610.1"
                     /translation="SLAVEEQFYLIYPPVLYALYRWARPLMWPVLALLALASLYVCQV
                     NLERYPAPAFYWPVSRAMELLIGALAAGAPPHLKLNVRAQSLLGGAGLIAILAGYALI
                     DDETPFPGLWALLPCLGTAALLLAPQGLPGRLLSAKPLVRVGDVSYSLYLWHWPLLVF
                     ARLLFPDAVWPVVAALLLSGVASWLSWRYIEAPFLRRPDKPIRWFALLCMAASIAACL
                     LIFYNGSLPQRFSPAARAYLAASNDYNHDRKTCHMESDKPIPYAQTCVYGDRRAAPAY
                     AVWGDSHGAELSRALGRKLGAYGQSLRELTMSGCPATLSRKPVCRQHNLDMMAAIKAD
                     PNMQTVILVGNLHDDDASARAATAGMEQTALDLQKAGKTAILVYPIPIYRFDPPSQLA
                     LSSRAQRPATTVGQPVSEYLARHRAIIDEFDRFTARNGIQHVEPQSLFCDDDLCHVYQ
                     PGVGVLYFNAGHLSMTGAGVLADRILSQLALSDQPEK"
BASE COUNT          567 a         1067 c         1149 g          495 t
ORIGIN      
        1 tggccgttct gcaacggcgc ctcgccgcgc cgcagcgccg tcgccacctc gttgagcgta
       61 tagccggacg gcggcggcga gggcgccttc agcaccagca gggtattgat ccccagcgcc
      121 agcaccagca cggtcaggga caggctcagc acctgccaga agatcggcag ggagacgaag
      181 aggggggggc gcccgcgcgc gttttgcggc atgatcgcgt tgtgtccgct atttcaaagg
      241 cttgaacata tagccgatgc cgcggatcgt gcggatgatg tcctcggcgt ggtcgtcgct
      301 gagcttgcgg cgcaggcgcg aaatctgcac gtcgatggcg cggtcgaagg aatcggcgtt
      361 cggcccgtgc aggtagtcga ggatctggtc gcgcgacagg gcgcggcccg gacggtcgag
      421 gaagacgcgc agcaactcga attcggcgtt ggacagcgcg acctgcacgc cgtcggggcg
      481 caaaagcgtg cgcttgatgt tgtcgagctg ccagccgaag aagtgggtga tcggcgactt
      541 ggcgtcccag ccgccgctgc gcgcctcgcg ccggcgcagc acggcgcgga tgcgcgccag
      601 cagttcgcgc gggtggaacg gcttggccat gtagtcgtcg gcgcccagtt cgaggccgac
      661 gatgcggtcg atctcctcgc cgcgcgccga cagcatgatc accggcggcc cgcccgcctc
      721 gttgagccgc ttgcacaccg acaggccgtc ctcgcccggc atcatgtagt cgagcacgat
      781 cacgtcgatg tcgccgcgcg ccagcagctt gtccatgtcg cgcgcctgcg acgcggtatg
      841 cacgacgaag ccgttgttgg acaggaactg gctgatcagc tcgcgcaggt cgggatcgtc
      901 gtcgacgatc agcgcgcggg tcggcgcatc gagggcggcg gcggggctgg ttagggacat
      961 gaaacgctcc gggtcatcac agggcctgca acctgaacgc ggcttgcggc gatctgattg
     1021 cgcgattgta acacgaaaac gccttgcggc ccaattcgtc ccgatcgctg acattcccgg
     1081 gcaacagggg cgcggcagga tgcgcgccgg acgacagacg cgccgggcgt ccgctttctg
     1141 tgaaggcgcg ggatgaggaa aaacatcatg ctgaggattt tggtcgtgac gggcgcgctg
     1201 gcgctgggtt cgtcgcttgc ggcgcgggcg caggagccgc cgccgcgccc cgacttcgcg
     1261 gcgctggacg ccaatcatga cggcaggctg gacctgtccg aattcaccgc gccgatgcag
     1321 gcgcgcgcca aggccgtgtt cgacaggatg gacgccaaac acgacggcgt cgtcaccgaa
     1381 agcgaattca acgccttccg ccccgaaaac ggtcctcccc gcgatggccc gccccgcatg
     1441 ggcccgctcc gcatgggacc gccgccggac ttcaggagcc tcgacgccaa cggcgacggc
     1501 aagatcaact tcgcggaatt ctcggcgcgc gaaaaagccc gcttcgaccg gctggacgcc
     1561 aaccacgacg gcgtgctgga gcccgccgaa ctgtccccgc cgaaggcggc gggaggagcc
     1621 gttcgcccct gaaaattttt tccgcgcatc gcgacagaat tttcctgaac gagcgttcaa
     1681 ttccgcgaac gtcgctcctc tggacggcgt aagaggccgt gcccgccggt atctccccgg
     1741 agccgtcgcg gaaacggaag ggccgtgaca gccgccgctg ccgcggccct tttttattct
     1801 ggcctttttt ctttattttt ccggctgatc gctcagcgcc agttgcgata ggatccggtc
     1861 ggccagcacg cccgctccgg tcatgctcag atggcccgca ttgaagtaca gcaccccgac
     1921 gccgggctga tagacgtggc acagatcgtc gtcgcagaac aggctctgcg gctcgacgtg
     1981 ctggatgccg ttccgcgccg tgaagcggtc gaactcgtcg atgatcgccc gatggcgcgc
     2041 cagatattcc gacacgggct gcccgaccgt cgtggcgggc cgctgcgcgc ggctggacag
     2101 ggccagttgc gacggcgggt cgaacctgta gatcgggatc ggatagacca gaatggcggt
     2161 tttgcccgcc ttttgcaggt cgagcgcggt ctgctccatg cccgccgtcg ccgcgcgcgc
     2221 cgaggcgtcg tcgtcgtgca ggttgccgac caggatcacg gtctgcatgt tcgggtcggc
     2281 cttgatcgcg gccatcatgt cgagattgtg ctggcggcag accggcttgc ggctcagcgt
     2341 ggccggacag cccgacatgg tcagctcgcg cagggattgc ccgtaggcgc ccagcttccg
     2401 gcccagcgcg cgcgacagtt cggcgccgtg gctgtcgccc cagaccgcat aggcgggcgc
     2461 ggcgcgcctg tcgccataga cgcaggtctg cgcgtagggg atcggcttgt ccgactccat
     2521 atggcaggtc ttgcggtcgt ggttatagtc gttcgacgcg gccagatagg cgcgcgctgc
     2581 gggcgaaaag cgctgcggca ggctgccatt gtaaaagatg agcaggcagg cggcgatgga
     2641 ggcggccatg cagaggagcg cgaaccaccg gatcggcttg tccggccgac gcaggaacgg
     2701 cgcctcgata tagcgccacg acagccagct cgccacgccg gacagcagca gggcggcgac
     2761 caccggccag accgcgtcgg gaaacaggag gcgcgcgaac accagcagcg gccagtgcca
     2821 caggtaaagc gaataggaga cgtcgccgac gcggaccagc ggcttcgcgc tcaacaggcg
     2881 gccgggcagg ccctgcggcg ccagcagcag ggcggcggtt ccgaggcagg gcaaaagcgc
     2941 ccaaaggccc ggaaacggcg tctcgtcgtc gatcagggcg taaccggcga gaatggcgat
     3001 cagacccgcg ccgcccagaa ggctttgcgc gcggacgttc agtttcaggt ggggcggggc
     3061 gcccgccgcc agcgcgccga tcagcaattc catggcgcgc gaaaccggcc agtagaaggc
     3121 cggggccggg taacgctcca gattgacctg gcagacatag agcgaggcca gcgccagcag
     3181 cgccagaacc ggccacatca gcgggcgcgc ccagcgataa agcgcgtaga ggaccggcgg
     3241 atagatcagg tagaactgtt cctcgaccgc cagcgacc
//
LOCUS       DASVLL010000590         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_151120,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000590 DASVLL010000000
VERSION     DASVLL010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151120"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VF402_09695"
     CDS             complement(<1..1153)
                     /locus_tag="VF402_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX7800611.1"
                     /translation="MKRHGLMLAAALTALMTAGGAMAQTPVDAPVPAPMPAPVAAPQD
                     VAYPGTLKLSVDATDLDHRLFAVHETLPVAQAGDFVVMLPKWLPGHHSPGTEIRRIAD
                     LFVSADGRTLSWTRDPLDMNAFHIQVPDGVKAVEVDFKYLSPVTAAAGRVTMTDAMID
                     LQWNFASLYPAGYYASRIPVEATVTLPKGWNYATGLETAARDGDVVRFKPTDYDTLVD
                     SPLMAGAYFKSWDLDPTGPVPVRLNVAADSPDMLNAPDDVIAIHKNLIQQAYKLYGSR
                     HYNHYDFLVFASDDLGGIGLEHHRSSEDSVDTDYFTDWKSAYVGRDLLAHEYTHSWNG
                     KFRRGADLWTPNFQVPMRDSLLWVYEGQTQYWGIVLGGRSGLYSKDQALQ"
     gene            complement(1230..1640)
                     /locus_tag="VF402_09700"
     CDS             complement(1230..1640)
                     /locus_tag="VF402_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7800612.1"
                     /translation="MADDKTIAAHVAETAQSLYAVAIDANGHGLVADEPLDFGGGNLG
                     PGPYDLLLSALGACTAMTVRWYAQKQGWPLVHVRVDLTHHKEGRADVFTKTVHLQGDD
                     LSPEQRATLVSVAEKCPVHRTLTSPETRIATTAG"
     gene            complement(1647..2510)
                     /locus_tag="VF402_09705"
     CDS             complement(1647..2510)
                     /locus_tag="VF402_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7800613.1"
                     /translation="MTSAVEMIIPARPKDLGGFEVRRVLPFAKRRKVGPWVFFDHMGP
                     VEFAPGPGVNVRPHPHINLATVTYLFEGQIWHRDSLGSDCVIAPGDVNLMAAGAGIVH
                     SERETEARQAQMRRLDGLQLWLALPEADEEMAAAFHHYASADIPRVDRDGVSLRVMMG
                     TAYGAVSPVRTFAETLYVEGRAAQGARVAAPDAAERAVYVCSGALEIDGQRVETYQMA
                     VLAPGAVEMTALSDAHFIVIGGEPLSERHIYWNFVSSRPERIEQAKADWKAGRFPRVP
                     GDEIEFIPLPE"
     gene            complement(2522..3277)
                     /locus_tag="VF402_09710"
     CDS             complement(2522..3277)
                     /locus_tag="VF402_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800614.1"
                     /translation="MRLRYSTYLLAALIVAAPVAGQAAVTPKALPAAKPAILLPDGGA
                     QTAQYDPAQGFPDRNGALSAMLVVIPQKETAVFAGQGEDRHIDRVSRAEPGANLAIKL
                     VFVGVRPDANGVANLTYDLKVTDPEGKLYAGSDYHNLTALIGQVGDAKAVYDNRDQVI
                     LMQFEPKDTPGVYKIDAVLHDRVAKLDLPLTASVELLRTGLPTPPATPAPGADATPQT
                     PAAQTPAAQTPAVQTPAKAAPKRVKHRRRRKHR"
     gene            complement(3367..>3549)
                     /locus_tag="VF402_09715"
     CDS             complement(3367..>3549)
                     /locus_tag="VF402_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX7800615.1"
                     /translation="ADQDEGVRLFTALGDAETLDFMRRHRDLIARFGRFPHRNAVLGR
                     RSTPDEAAALADGARF"
BASE COUNT          597 a         1213 c         1148 g          591 t
ORIGIN      
        1 attgcagcgc ctgatccttg ctgtagaggc ccgagcgccc gcccagcacg atgccccagt
       61 actgcgtctg gccctcatag acccacaata gcgagtcgcg catcggaacc tggaagttcg
      121 gcgtccacag atcggcgccg cgccggaact tgccgttcca cgaatgggtg tattcgtgcg
      181 ccagcagatc gcgcccgaca taggcgctct tccagtcggt gaaatagtcc gtatcgacgc
      241 tgtcctcgga cgagcgatga tgctccagcc cgatcccgcc cagatcgtcc gaggcgaaaa
      301 ccagaaaatc gtaatgatta taatggcgtg aaccgtaaag cttataggcc tgttgaatca
      361 ggtttttatg gatggcgatc acgtcgtccg gcgcgttcag catgtccggg ctgtcggcgg
      421 cgacgttcag ccgcaccgga accgggccgg tgggatcgag atcccagctt ttgaaatagg
      481 ccccggccat cagcggcgaa tcgaccagcg tgtcgtaatc ggtcggcttg aaccgcacca
      541 cgtcgccgtc gcgcgccgcc gtttccagcc cggtggcgta gttccagccc ttgggcagcg
      601 tcaccgtcgc ctcgaccggg atgcggctgg cgtaatagcc cgccgggtac agcgaggcga
      661 agttccattg caggtcgatc atcgcgtccg tcatggtgac gcgccccgcc gccgccgtca
      721 ccggcgacag gtatttgaaa tcgacctcga ccgccttcac gccgtcggga acctgaatat
      781 gaaaggcgtt catatcgagc ggatcgcgcg tccagctcag cgtccggccg tccgcgctga
      841 cgaacaggtc ggctatgcgg cgaatctccg tgccgggcga atggtggccg ggcagccatt
      901 tcggcagcat gacgacgaaa tcgcccgcct gcgcgaccgg cagggtttca tgcacggcga
      961 agaggcgatg atcgaggtcg gtggcgtcga cgctgagttt gagcgtcccc ggataagcga
     1021 cgtcctgcgg cgccgcgacc ggcgcaggca tgggcgccgg aacgggcgcg tccaccggcg
     1081 tctgggccat cgcgccgccc gccgtcatca gcgccgtcaa cgccgccgcc agcatcaaac
     1141 cgtgtctttt catcgcacaa cccgctgaaa aagctaaacc ctcgcgggcc agccaagcgc
     1201 agatcgtttc aaatgtaaag tcaaaagcct cagcccgccg tggtggcgat gcgcgtctcc
     1261 ggcgaggtca gggtgcgatg caccgggcat ttctccgcca ccgaaaccag cgtagcgcgc
     1321 tgctcgggac tcagatcgtc gccttgcaga tgcaccgtct tggtgaagac gtccgcccgc
     1381 ccctccttgt gatgcgtcaa atcgaccctg acatggacca gcggccagcc ctgcttctgc
     1441 gcgtaccagc gcacggtcat cgccgtgcag gcgcccagcg ccgacaacag cagatcgtaa
     1501 ggccccggcc ccagattgcc gccgccgaaa tccagcggct cgtccgccac cagcccgtga
     1561 ccgttggcgt cgatggcgac cgcatagagg gattgcgccg tctcggcgac gtgcgcggcg
     1621 atggttttgt cgtcggccat gtttgtttac tcgggcagcg ggatgaattc gatctcgtca
     1681 ccgggaaccc tggggaagcg cccggccttc cagtcggcct tggcctgctc gatgcgctct
     1741 ggccgcgacg acacgaagtt ccagtagata tggcgctcgc tcagaggctc gccgccgatc
     1801 acgatgaaat gcgcgtcgct cagcgccgtc atttccaccg cgccgggcgc cagcaccgcc
     1861 atctgatacg tctcgacgcg ctgaccgtcg atctccagcg cgcccgaaca gacatagacc
     1921 gcgcgctcgg cggcgtccgg cgcggcgacg cgcgcgccct gagcggcccg gccctcgaca
     1981 taaagcgttt cggcgaaggt tctgaccggc gagaccgcgc cataggcggt ccccatcatg
     2041 acgcgcaggc tcacgccgtc gcggtcgacg cgcggaatgt ccgccgaggc gtaatgatgg
     2101 aacgccgccg ccatctcctc gtcggcttcc ggcagggcca gccacagttg cagcccgtcc
     2161 agccgccgca tctgcgcctg ccgcgcctcg gtctcgcgct cggaatggac gatgcccgcg
     2221 cccgccgcca tcagattgac gtcgcccggc gcgatcacgc agtcgctgcc cagcgagtcg
     2281 cggtgccaga tctgtccctc gaacagatag gtcacggtgg ccagattgat gtgcggatgc
     2341 ggccggacat tgacgcccgg ccccggcgcg aactccaccg gccccatatg gtcgaaaaag
     2401 acccacggcc cgaccttgcg acgcttcgcg aacggcagca cacggcgcac ctcgaacccg
     2461 cccagatcct tggggcgcgc gggaatgatc atctcgacgg cggaggtcat gatcgtggct
     2521 cctaacgatg cttgcggcgg cggcggtgct tgacgcgctt cggggccgcc ttggccggag
     2581 tctggaccgc aggcgtctgg gcagcggggg tctgggccgc cggggtctgc ggcgtcgcat
     2641 ccgcgcccgg agccggagtc gcgggcggcg ttggcagccc cgtgcgcagc agttccacgc
     2701 tggccgtcag cggcagatcc agcttggcga cccggtcgtg cagcaccgcg tcgatcttgt
     2761 aaacgcccgg cgtgtccttc ggctcgaact gcatcaggat cacctgatcg cggttgtcat
     2821 agaccgcctt cgcatcgccg acctgcccga tcagcgccgt caggttatgg tagtcgctgc
     2881 ccgcgtacag cttgccctcg ggatcggtga ccttgagatc gtaggtcagg ttggcgaccc
     2941 cgttggcgtc gggcctgacg ccgacgaaga ccagcttgat cgccagattg gcgcccggtt
     3001 cggcgcggct gacgcggtcg atatgccggt cctcgccctg ccccgcgaaa accgccgttt
     3061 ccttttgcgg aatgacgacc agcatggccg acaaggcgcc gttgcggtcg ggaaagccct
     3121 gcgccggatc gtattgcgcc gtctgcgcgc cgccgtcggg caacaggatg gcgggcttgg
     3181 cggcgggcag ggctttcggc gtcacggcgg cctgtccggc caccggcgcc gcgacgatca
     3241 gggcggccag aagatatgtg gaataacgta agcgcattcg aggttccatg agcccaaccc
     3301 gtccagctta tgtcatgggg ggcggatcgc ggcaagtcta tgacgccggc ctaaaaagac
     3361 gccggcttaa aaacgcgcgc cgtcggccag cgccgccgcc tcgtccggcg tgctgcgccg
     3421 tcccagaacc gcgttgcgat gcggaaaccg cccgaagcgc gcgatcaggt cgcgatggcg
     3481 gcgcatgaaa tcgagcgtct cggcgtcgcc caaagccgtg aacagccgca cgccctcgtc
     3541 ctgatcggc
//
LOCUS       DASVLL010000591         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_416062,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000591 DASVLL010000000
VERSION     DASVLL010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416062"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..2037)
                     /locus_tag="VF402_09720"
     CDS             complement(370..2037)
                     /locus_tag="VF402_09720"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7800616.1"
                     /translation="MTALATLAYAGRAWPFEQARALIARLVKSRVTGHEAQKQALSLI
                     EAGKTDEALKAFEGLDRAVIFETGYGPSGLPHIGTFGEVARTTMVRQAFEAMTGGVWA
                     TRLICFSDDMDGLRKVPDGISNPEVLREDLYKPLTVVRDPFGDYPSFGQHNNARLKAF
                     LDGFGFAYDFMSATDCYKGGMFDQTLLVALERFDDIQKIMLPTLGPDRRATYSPFLPI
                     SPKTGHVLQVPTLERNVGKGTIVFEENGERFEVPVTGGHVKMQWKPDWAMRWAALGVD
                     YEMAGKDLIDSVKVSSQVCRVLGGTPPEGFNYELFLDENGQKISKSKGNGLTMEDWLR
                     YGAPESLAYYMFQSPKSAKKLYFDVIPRATDEFFQQLDAFPKQELDKRIENPVWFVLR
                     GQEAPKSPPVTFGLMLNLVSAANAADKATLWGFLRAYIPGATPESEPVLDRLAGYALN
                     YFEDFVRPAKTFRLPDDRERAAMLDLAARLRALPAGTRDAEALQNIVFEVGKAHEFEP
                     LRAWFQALYEVLLGQSQGPRFGSFVAIFGVERTVALIEQALAGELAA"
BASE COUNT          438 a          762 c          630 g          375 t
ORIGIN      
        1 caaaggcggc ttcggacctg acgtcattgg acaggatcag cacgcaacgc ttcgaatcct
       61 caaggcacac catcgtattg gcggtctggc cgtcgtgtcc gcccttgtaa aagcccggcc
      121 cctgcggccc cttgaacgtc acgacgccca gccccggagg gcatggcgtt gccaggatct
      181 ggtgcgacgg cggggacagc gagggcgaga ctgaacgcga ggagacggcg catgaacgga
      241 ctccgtagcg aatggggttt ggggccaatt ggccccaagt cttcccttct tatcctgcct
      301 atggcccaag ggccataggc aggataagaa gaaaggatgt gggggcaatc gcccccacgc
      361 cccattcgat tacgcagcca gttcccccgc cagcgcctgc tcgatcaacg ccaccgtccg
      421 ctccacgccg aaaatcgcca cgaacgagcc gaagcgcggc ccctgcgatt gccccagcag
      481 cacctcatac agcgcctgga accatgcccg caaaggctcg aactcatgcg ccttcccgac
      541 ctcgaacacg atgttctgca aggcttcggc gtcgcgcgtc ccggcgggca gagcgcgaag
      601 ccgcgccgcc agatcaagca tcgccgcccg ctccctgtcg tccggcaaac ggaaggtctt
      661 ggccggccgc acgaagtcct cgaaatagtt gagcgcataa cccgccagac ggtccagcac
      721 cggctcggac tccggcgtcg cgcccggaat ataggcgcgc aggaagcccc acaatgtagc
      781 cttgtccgcc gcattggccg ccgacacgag gttcagcatc agcccgaacg tcaccggcgg
      841 cgatttcggc gcctcctgcc cgcgcagcac gaaccacacc gggttctcga tgcgcttgtc
      901 cagttcctgc ttcgggaagg cgtcgagctg ctggaagaac tcgtccgtcg cgcgcggaat
      961 cacgtcgaaa tacagcttct tggccgattt cggcgactgg aacatatagt aggccagact
     1021 ctccggcgcg ccgtaacgca gccagtcctc catcgtcagg ccgttgccct tcgacttgga
     1081 aatcttctgg ccgttctcat cgaggaacaa ctcgtaattg aagccttccg gcggcgtccc
     1141 gcccagcacg cggcagacct gcgacgacac cttgaccgag tcgatcagat ccttgcccgc
     1201 catctcgtaa tcgacgccca gcgccgccca gcgcatcgcc cagtccggct tccactgcat
     1261 cttgacgtgg ccgcccgtga ccggaacctc gaacctttcg ccgttctcct cgaacacgat
     1321 cgtgcccttg ccgacattgc gctccagcgt cggaacctgc aacacatggc cggttttcgg
     1381 cgagatcggc agaaacggcg aataggtcgc gcgccggtcc ggccccaggg tcggcagcat
     1441 gatcttctgg atgtcgtcga accgctccag cgccaccagc agggtctggt cgaacatccc
     1501 gcctttgtaa caatcggtcg ccgacatgaa gtcgtaggcg aaaccgaaac cgtcgagaaa
     1561 cgccttcagc cgcgcattgt tatgctggcc gaaggacgga tagtccccga acggatcgcg
     1621 caccacggtc agcggcttat acaggtcttc ccgcagaacc tccggattgg aaatgccgtc
     1681 cggcacttta cgcaggccgt ccatatcgtc ggaaaaacag atcagccgcg tcgcccagac
     1741 gccgccggtc atcgcctcga acgcctgccg caccatggtc gtgcgcgcca cctcgccgaa
     1801 ggtgccgata tgcggcaggc ccgacggccc gtagccggtc tcgaagatca ccgcgcggtc
     1861 cagcccctcg aacgctttta acgcctcgtc cgtcttgccc gcctcgatca ggctcagcgc
     1921 ctgtttctgc gcctcatgcc ccgtcacgcg cgatttgacc aggcgggcga tcaacgcgcg
     1981 agcctgttca aacggccatg cgcggccggc gtaagcgagg gtggcgagag cggtcatgaa
     2041 gaaggtccaa acctttataa aacaatcgga gcgcccccat tagaacccat gcgcgccggg
     2101 gtcaagccag cccggtcttg acggcgcgcc gatttaagga taccagacaa ctttaaggat
     2161 atatttcatc cttattatga aacggaggcc gtcatgagcg cacgc
//
LOCUS       DASVLL010000592         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_39292, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000592 DASVLL010000000
VERSION     DASVLL010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39292"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VF402_09725"
     CDS             <1..1219
                     /locus_tag="VF402_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7800617.1"
                     /translation="WRSTETNAFGTNEFIAWCRQAEVEPMFAVNLGTRGPDAARHFLE
                     YCNHPGGTYYSDLRRSHGHEQPHGIKFWCLGNEMDGPWQTCAKTAQEYGRIAQETGKV
                     MKWVDDTIQLAACGSSQRAMPTYAAWEYEVLDACFDQVDYISLHHYMRNDSDDIREFF
                     AEIESLDYFIREVAAIADAVAAKRRSKKRIMLSVDEWNVWYKAHTPDDVKKPGWPKAP
                     PLLEEIYNFEDALVVGGALIVMMNNADRVKTACLAQLVNVIGPIMTETGGPAWRQTIF
                     HPIADCTRHGRGEVLRLKVESPTFATKAHGQIPCLLATAVFDPESGRVSLFALNRSTD
                     EAMDLSLDLRGLGDGLAVIEAHELHETDLKKSNGKAAPDAVAPRPQTTGAMSGTVFRA
                     RLKPQSWNVFVLG"
     gene            1518..>4260
                     /locus_tag="VF402_09730"
     CDS             1518..>4260
                     /locus_tag="VF402_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800618.1"
                     /translation="MTDATSAARRGRHFRLHRAGLAVAASALALGFALHAQAQDAAAA
                     SAAAPTDDSTVVIVKGVRASLTKALSIKRKSTQVVEAVVAEDIGKLPDNNVVEALQRV
                     TGVQVTNRAGGEVGTISIRGLPDINTTWNGRNIFTASGTQVALQDIPATLVREIDVYK
                     TRDASQIPTGIAGQVDVKTLRPLDFKGPEVSIAARATYLDPAKKWNPQLSALLSDRWS
                     TSHGEFGALINLSDVKTDYRNESVTPGALVVFASADPSQVPAGWGTLERIQPTDARAP
                     NQQIWTPGLHQGLSEKEGSTLNINGVDVPYYLSRDAIFQDDVQGQRDRPAANIALQWQ
                     PNADATYTFEAMYNGYRDKNLSALLFSFVDGWWDDGATPANSITFFPGTNIIKTRTVG
                     SVYGFNSGDYTTSATDSYVYALGGEWNVGDRLKLNGDLSYQNSVYHSEFTATRIERTA
                     PSVTVDFNAGDDSMAFHFNNDADLLDPTKWTVAQFYDNANRNEGSAATATFNGVYDAD
                     WGALKTLSFGARYDDRKASEANRTQSSYLGKSLASLDSSYLWTNSGFGTDISDVPRAW
                     LEPNGAYIRDHIDAWRAMYAAVDPNFLTTDELHLQKTFDVDEKTAEVYLMGDTDNEVF
                     GHRLRGNFGVRYVNVQTNMNFYSVDATTKAVTVSSASKTASKALPSLTLRYDLSDDVL
                     LRLNYGETLRRPNFGDLNPVVQYGDDVSKVGYGSGSGGNPNLNPTQSKNLDLTAEWYF
                     SKDSALYGTLFTRTIDGLVVSLLHKVHVDRADDPFKNSTSGGDHSNGYDYVINTPVNA
                     SNGKISGAELGLIWFPKNLPNVLNGLGFQGSVTTLASSQNVPDTDAAGNIIAQLKTPF
                     FGVSRYSYNATLAYDKGPIGARLSYVWRSSFLASNEASLFANPLGIWRHPEKSLDFQL
                     "
BASE COUNT          854 a         1475 c         1257 g          674 t
ORIGIN      
        1 ctggcgttcg accgagacca acgccttcgg gaccaacgaa ttcatcgcct ggtgccgtca
       61 ggccgaggtc gagccgatgt tcgcggtcaa tctgggcacg cgcggcccgg acgcggcgcg
      121 tcatttcctc gaatactgca accatccggg cggaacctac tattccgacc tgcgccggtc
      181 gcacggccat gagcagccgc acgggatcaa gttctggtgc ctgggcaacg agatggacgg
      241 gccgtggcag acctgcgcca agaccgcgca ggaatacggc cgcatcgcgc aggagaccgg
      301 caaggtgatg aagtgggtcg acgacacgat ccaactggcc gcctgcggtt cgtcgcaacg
      361 cgccatgccg acctatgcgg cgtgggagta cgaggtgctg gacgcctgct tcgatcaggt
      421 cgactacatc tcgctgcacc actacatgcg caacgattcc gacgacatca gggagttctt
      481 cgccgagatc gagagcctcg actatttcat ccgcgaggtc gccgccatcg ccgacgccgt
      541 ggcggccaag cgccgctcga agaagcgcat catgctgtcg gtcgacgaat ggaacgtctg
      601 gtacaaggcg cacacgcccg acgacgtgaa aaagcccggc tggcccaagg cgccgccgct
      661 gctggaggag atctacaatt tcgaggacgc gctggtcgtc ggcggggcgc tgatcgtgat
      721 gatgaacaac gccgaccgcg tcaagacggc gtgcctggcg caactggtca acgtcatcgg
      781 gccgatcatg accgagaccg gcggcccggc gtggcggcag accatcttcc acccgatcgc
      841 cgactgcacg cgccacggac gcggcgaggt cttgcggctg aaggtcgagt ccccgacctt
      901 cgcgacgaag gcgcacggcc agattccctg cctgctggcc acggccgtct tcgatcccga
      961 atcgggccgg gtcagcctgt tcgcgctcaa ccgctccacc gatgaggcga tggatctgtc
     1021 gctggacctg cgcggcctcg gcgacgggct ggcggtgatc gaggcgcacg aactgcacga
     1081 aaccgacctg aaaaagagca acggcaaagc ggcccccgac gccgtcgcgc cgaggccgca
     1141 gaccaccggc gccatgtccg ggacggtctt tcgcgcccgc ctcaagccgc agtcatggaa
     1201 cgtttttgtc ctgggataat tgtcctacca gaacaaagcc gctgacctga caattaagcc
     1261 gcgattttta catcagaaaa aagcggcttt tcgtttccac acatgaatta ttcgccgcca
     1321 aagcccgaaa tacgacaaga taccggtgtc ttgcaccgaa gctcgtccat cgcgcgccat
     1381 gttgccgaat tgagacagcc gcctttaatc agacaaataa acgccgtgtc tattgacaca
     1441 ctttttttgt cggacaaata tcgcgccaaa gtccgccagc ataaagcgga tgaggactct
     1501 aactgggaga tacatacatg actgacgcta cgtcagcggc ccgccgcggc cgtcatttcc
     1561 gcctccatcg cgccggtctg gccgtggccg cctcggccct ggcgctgggc ttcgcgctgc
     1621 acgcccaggc gcaggacgcc gccgccgcgt ccgccgccgc gccaaccgac gattcgaccg
     1681 tcgtgatcgt caagggcgtc cgggcgtcgc tgaccaaggc cctgtcgatc aagcgcaagt
     1741 cgacccaggt ggtcgaagcg gtcgtcgccg aggacatcgg caagctgccc gacaacaacg
     1801 tggtcgaggc gctgcaacgc gtgaccggcg tgcaggtgac caaccgcgcc ggcggcgaag
     1861 tggggaccat ctcgattcgc ggcctgccgg acatcaacac gacctggaac ggtcgcaaca
     1921 tcttcaccgc ctcgggcacg caggtcgcct tgcaggacat ccccgcgacc ctggtgcgcg
     1981 agatcgacgt ctacaagacc cgcgacgcca gccagattcc gaccggcatc gccggtcagg
     2041 tcgacgtcaa gaccctgcgc ccgctcgact tcaagggacc ggaagtctcc atcgccgcgc
     2101 gcgcgaccta tctcgacccg gccaagaagt ggaatccgca actcagcgcg ctgctcagcg
     2161 accgctggtc cacctcgcac ggcgaattcg gcgccctgat caatctcagc gacgtcaaga
     2221 ccgactatcg caacgaaagc gtgactccgg gcgcgctcgt cgtattcgcc agcgccgatc
     2281 cgagccaggt tcccgccggt tggggcacgc tcgagcgcat tcaacccacg gacgcgcgcg
     2341 cgcccaacca gcagatctgg acgccgggcc tgcatcaggg cctgtcggaa aaggaagggt
     2401 ccaccctcaa catcaacggc gtcgacgtgc cgtattatct gtcccgcgac gccatcttcc
     2461 aggacgacgt acagggtcag cgcgaccggc cggccgccaa tatcgccctg caatggcagc
     2521 ccaacgccga cgcaacctac acgttcgagg ccatgtacaa cggctatcgc gacaagaacc
     2581 tcagcgccct gctgttcagc ttcgtcgacg gctggtggga cgacggcgcc acgccggcga
     2641 acagcatcac cttcttcccc ggcacgaaca tcatcaagac gcgcaccgtc ggctccgtct
     2701 acggcttcaa ttcgggcgac tacaccacct cggcgaccga ttcctacgtc tacgcgctgg
     2761 gcggcgaatg gaatgtcggc gaccggctga agctgaacgg cgatctgtcc tatcagaaca
     2821 gcgtctatca ttcggaattc accgccaccc gcatcgaacg caccgcgccg tccgtcaccg
     2881 tcgacttcaa cgcgggcgac gacagcatgg ccttccattt caataacgac gccgatctgc
     2941 tcgacccgac gaagtggacg gtggcgcaat tctacgacaa cgccaaccgc aacgaaggca
     3001 gcgcggcgac cgcgaccttc aacggcgtgt acgacgccga ctggggcgcg ctcaagaccc
     3061 tgagcttcgg cgcgcgctac gacgaccgca aggcgtcgga agccaaccgc acgcagtcgt
     3121 cctatctggg caagagcctc gcctccctgg attcgtccta cctgtggacc aattcgggct
     3181 tcgggaccga tatttccgac gtgccgcgcg cttggctcga accgaacggc gcctatatcc
     3241 gcgaccatat cgacgcatgg cgcgccatgt acgcggcggt ggacccgaac ttcctgacca
     3301 ccgacgagct gcatctgcaa aagaccttcg acgtcgatga aaagaccgcc gaagtctacc
     3361 tgatgggcga tacggacaac gaggtcttcg gtcatcgcct gcgcggcaat ttcggcgtgc
     3421 gctacgtcaa cgtgcagacg aacatgaact tctacagcgt cgacgccacg accaaggcgg
     3481 tcacggtcag ctcggcctcc aagacggcca gcaaggccct gcccagcctg accctgcgct
     3541 acgacctgag cgacgacgtg ctgctgcggc tgaactacgg cgagaccttg cgccgcccga
     3601 acttcggcga cctcaatccg gtcgtgcagt acggcgacga cgtctccaag gtcggctacg
     3661 gctccggctc cggcggcaac ccgaacctga acccgacgca gtccaagaac ctcgacctga
     3721 cggcggagtg gtacttctcg aaggacagcg cgctttacgg caccctgttc acccgcacca
     3781 tcgacgggct ggtcgtcagc ctgctgcaca aggttcacgt cgaccgcgcc gacgatccgt
     3841 tcaagaactc gacctcgggc ggcgaccaca gcaacggcta cgactacgtg atcaacaccc
     3901 cggtcaacgc gtcgaacggc aagatcagcg gcgcggagct gggcctgatc tggttcccga
     3961 agaacctgcc gaacgtcctg aacggcctgg gcttccaggg cagcgtcacc acgctggcct
     4021 cctcccagaa cgtgccggac accgacgcgg cgggcaatat catcgcccag ctcaagacgc
     4081 ccttcttcgg cgtgtcgcgc tactcctaca acgcgaccct ggcgtatgac aaaggcccga
     4141 tcggcgcgcg cctgtcctat gtctggcgct ccagcttcct ggccagcaac gaggccagcc
     4201 tgttcgccaa cccgctgggc atctggcgtc atccggaaaa gagcctggac ttccagttga
//
LOCUS       DASVLL010000593         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_362572,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000593 DASVLL010000000
VERSION     DASVLL010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362572"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VF402_09735"
     CDS             <1..872
                     /locus_tag="VF402_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX7800619.1"
                     /translation="KVKRFETSGDGRTVAAVVTESADGLLSSFRGDIFVLAAGAANSA
                     AVLLRSRNEHHPDGLANASGLVGRNYMFHTTSAVISIMAERFDSTFPKTFAVNDFYFG
                     EPDYPYPMGQIQLLEYMNGQTLEGQISDMIPPALIPDFLTDDIARHMVAFLTMSEDLP
                     RFENRISLTDDDQIKLTYTPNNLTSHLRLTKKLEAGLKGFSLRQHTIFEPHFAIDSLL
                     PLYGTAHQCGTIRFGRDPASSVLDENCKAHELDNLYVTDTSFFVSASAVNPTLTTVAN
                     ALRVGDVLKDRLG"
     gene            complement(869..1249)
                     /locus_tag="VF402_09740"
     CDS             complement(869..1249)
                     /locus_tag="VF402_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7800620.1"
                     /translation="MKVVLDASAVLALILREPGADRVQDEIGDAAISAVNMAEVFARC
                     QSRGLDPARIGGICVDLGLETLPFTAAHALLAGQLRASTRALGLSLGDRACLATAMLE
                     KRPVLTADRAWLRLDLGVEIVSIR"
     gene            complement(1246..1566)
                     /locus_tag="VF402_09745"
     CDS             complement(1246..1566)
                     /locus_tag="VF402_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7800621.1"
                     /translation="MPRNRLYKSAPDPQASGVDEAPAAPFHLQLQIGSDGRVLIPAEL
                     RRQMKIDASGRLTARIVDGELRLISPVAALDELWRYVRENDKGTGSAVDELIAERRAE
                     AARE"
     gene            complement(1618..>1810)
                     /locus_tag="VF402_09750"
     CDS             complement(1618..>1810)
                     /locus_tag="VF402_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7800622.1"
                     /translation="ILHGMKDEVVPWAHGVRLMETLTARDVRMELIKDGDHRLSRPQD
                     LQRLGEMAAELHNLALREN"
BASE COUNT          324 a          633 c          546 g          307 t
ORIGIN      
        1 ataaggtcaa gcgcttcgag acgtccggcg acggcaggac ggtcgccgcc gtcgtcaccg
       61 aaagcgccga cggcctgttg tccagcttca ggggcgacat cttcgtgctg gcggcgggcg
      121 cggccaattc cgccgccgtg ctgctgcgtt cgcgcaacga acaccacccg gacgggctgg
      181 ccaacgcctc gggcctcgtc gggcgcaact acatgttcca caccacctcg gcggtgatct
      241 cgatcatggc cgagcgcttc gattcgacct tccccaagac cttcgcggtc aacgacttct
      301 atttcggcga gccggactat ccgtacccga tggggcagat tcaactgctg gaatacatga
      361 acggccagac gctggagggg cagatctccg acatgatccc gcccgcgctg atccccgact
      421 tcctgaccga cgacatcgcg cgccacatgg tcgccttcct gaccatgagc gaggatctgc
      481 cgcgcttcga gaaccggatc agcctgaccg acgacgacca gatcaagctg acctatacgc
      541 cgaacaacct gacctcgcac ctgcggctga ccaagaagct ggaggcggga ctgaagggct
      601 tttcgttgcg ccagcacacg atcttcgagc cgcatttcgc catcgattcg ctgctgccgc
      661 tgtacggcac cgcgcatcag tgcggcacga tccgcttcgg gcgcgacccg gcgtccagcg
      721 tgctggacga aaactgcaag gcgcacgagc tggacaatct ctacgtcacc gacacctcgt
      781 tcttcgtgtc ggcctcggcg gtcaacccga ccctgaccac ggtggcgaac gcgcttcgcg
      841 tcggggacgt tctcaaggat cggctgggtt agcggatcga cacgatctcg acgccgagat
      901 cgagcctgag ccacgcccgg tccgccgtca gcacgggccg cttttccagc atggccgtcg
      961 ccagacaggc gcggtcgccc agcgacagcc caagcgcgcg cgtcgacgcg cgcaactgtc
     1021 cggccagcaa ggcgtgcgcc gccgtaaagg gcagggtttc cagacccaga tcgacgcata
     1081 tgccgccgat cctcgccgga tcgaggccgc ggctctgaca acgcgcgaaa acctcagcca
     1141 tattcaccgc gctgatcgcg gcgtcgccaa tctcgtcctg aacgcggtcg gcgcccggct
     1201 cgcgcaagat caaagccaga acggcggacg catccagaac gaccttcatt cgcgggcggc
     1261 ctcggcgcgg cgttcggcaa tcagttcatc gaccgccgat ccggtcccct tgtcgttttc
     1321 gcgcacatag cgccatagct catccagcgc cgcgaccggg ctgatcaggc gcaactcgcc
     1381 gtcgacgatc cgcgccgtca ggcggccgct ggcgtcgatc ttcatctggc ggcgcagttc
     1441 ggcggggatc agcacccgac cgtccgaacc gatctgcaat tgcaggtgaa aaggcgcggc
     1501 cggcgcttcg tcgacgccgg aagcctgcgg gtcgggcgcg cttttgtaca ggcggttgcg
     1561 gggcatggcg gcctctctgt caataacggc attttatgcc atattgtgcg gaaactgtca
     1621 attttcgcgc aaagccagat tatgcaactc cgccgccatt tcgcccagac gctgcaagtc
     1681 ctgcgggcgc gacaggcggt ggtcgccgtc cttgatcagc tccatgcgca cgtcccgcgc
     1741 ggtcagcgtc tccataagcc gcacgccatg cgcccacggc acgacctcat ccttcatgcc
     1801 gtgcaggatg
//
LOCUS       DASVLL010000594         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_201579,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000594 DASVLL010000000
VERSION     DASVLL010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201579"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VF402_09755"
     CDS             complement(<1..142)
                     /locus_tag="VF402_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7800623.1"
                     /translation="MYGFKSSHIRGSMSFKILPTIMSGGSGTRLWPLSTTEAPKQFHA
                     LAT"
     gene            complement(164..313)
                     /locus_tag="VF402_09760"
     CDS             complement(164..313)
                     /locus_tag="VF402_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800624.1"
                     /translation="MKKPLMAHEWRDVYNALHKEAVDLFQGWLKFVFQKYLFLRQFLL
                     GSGRR"
     gene            312..1472
                     /locus_tag="VF402_09765"
     CDS             312..1472
                     /locus_tag="VF402_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX7800625.1"
                     /translation="MTDLATRLNVRQTRLRQWLFDHALPIWWNVGGDRDKGGFFEKLN
                     LDGSPCDVDRRTRVAARQVFSYALADRMGYAGETAAAVDQGLNWLSGPARNPDTGLLY
                     AVLTPNGEVVKGAFDFYDHAFAMLAYASAFRARPAQQGLQDAGVFIRDRIVAGYAHPQ
                     RGFEESNPPTLPLKANPHMHMFEACLAWLDAGGDAEWERIAAMIADLCLDRFLHPQTG
                     ALREFFDADWNPMPGEAGRVLEPGHQFEWAWLFMRWSQRTSDAKYLRAAQTLMQIAET
                     AGTDPVRGVTFNELWDDFTPKDKNARLWPQTERVKAYVQLSRMLSGPDRDGAIGKAVE
                     AADGLLLYLSTEIPGLYRDRMKADGSFVEEPAPASSLYHIVCAIDELCAIAA"
     gene            1549..1977
                     /locus_tag="VF402_09770"
     CDS             1549..1977
                     /locus_tag="VF402_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800626.1"
                     /translation="MRRVLILEDSPTQATIISKMFQRAGFDPVCARDKSAALAALAAQ
                     GFDLLVLDVFIENSNTLDDLEVYRELAADAPIAVMTAGRIDNPEAGADALNKARRARV
                     SFLLPKPFFFVDVKQVCEDVEAHWSHAAPIPPSAAQRIAG"
     gene            1988..2596
                     /locus_tag="VF402_09775"
     CDS             1988..2596
                     /locus_tag="VF402_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800627.1"
                     /translation="MSHASLRDAFGLSEADISNLMGLMRQSETLVGLMDDFFGKLDTR
                     RSQELLNIAAAIAEAKTELRALRPRGLPSQGLNGANQELDAIVRDAEDATNAILNSAE
                     AILEMDPETGGLKDKVDDQVMRIFEACSFQDIIGQRVSKIVTLLAKIETCLGDMAERL
                     GAPEPDAAPLTPEDRRRRDLMLNGPALNGPEVAQDAIDALFV"
BASE COUNT          501 a          918 c          823 g          436 t
ORIGIN      
        1 acgtcgccag cgcgtgaaac tgcttcggcg cctcggtcgt cgacaggggc cacaggcgtg
       61 tgccggagcc gccggacatg atcgtaggca ggattttaaa cgacatggaa cctcgaatat
      121 gactggattt aaacccgtac atgaatggcg cggcataaaa acgtcatcgc cttccgctgc
      181 caagcaaaaa ttggcgcaag aataaatatt tttgaaatac aaattttagc catccttgaa
      241 acaaatcgac ggcttcttta tgcagagcgt tataaacatc gcgccattca tgcgccatca
      301 ggggcttttt catgaccgac ctcgccaccc gcctgaacgt ccggcagacc cggttgcggc
      361 aatggctgtt cgaccacgcc ttgccgatct ggtggaatgt cggcggcgac cgggacaagg
      421 gcggcttttt cgagaagctc aatctcgacg gctcgccctg cgacgtcgac cggcgcacgc
      481 gcgtcgcggc gcgtcaggtg ttttcttacg cgctggccga ccgcatgggc tatgcgggcg
      541 agacggcggc ggcggtcgat cagggcctga actggctgtc cggcccggcg cgcaatcccg
      601 acaccggcct cctgtacgcc gtgctgacgc ccaatggcga ggtggtgaag ggcgccttcg
      661 acttctacga ccacgccttc gccatgctgg cctacgcctc ggcgttccgc gcgcgccccg
      721 cgcaacaggg gctgcaagac gccggcgtct tcatccgcga ccgcatcgtc gccggttacg
      781 cccatccgca gcgcggcttc gaggagtcca atccgcccac cctgccgctc aaggccaatc
      841 cgcacatgca catgttcgag gcgtgcctgg cgtggctcga cgcgggcggc gacgccgaat
      901 gggagcgcat cgccgccatg atcgccgacc tgtgcctgga caggtttctg cacccgcaga
      961 ccggcgccct gcgcgaattc ttcgacgccg actggaaccc gatgccgggc gaggcgggcc
     1021 gcgtcctgga gcccggccat cagttcgagt gggcgtggct gttcatgcgc tggtcgcaac
     1081 gcacgagcga tgcgaaatat ctgcgcgccg cccagaccct gatgcagatc gccgagacgg
     1141 cgggcacgga cccggtgcgc ggcgtgacct tcaacgagct gtgggacgac ttcacgccca
     1201 aggacaagaa cgcccgcctg tggccgcaga ccgagcgcgt caaggcctat gtccagctca
     1261 gccgcatgct ctccggcccc gaccgcgacg gcgccatcgg caaggcggtc gaggccgccg
     1321 acggcctgct gctttatctg tcgaccgaaa ttccgggcct ctaccgcgac cggatgaagg
     1381 ccgacggctc tttcgtcgag gagcccgcgc ccgccagttc gctctatcat atcgtctgcg
     1441 ccatcgacga attgtgcgcg atcgcggcgt aaggacgcag aacaacaata ttttaaactg
     1501 tctgacctaa tctgtccgct ccgggtgtac agacaaagga agaccgcctt gcgacgcgta
     1561 cttattctcg aagacagccc gacccaggcc accatcatca gcaagatgtt ccagcgcgcc
     1621 ggattcgacc cggtctgcgc gcgcgacaaa agcgcggctc tggcggctct ggccgcgcaa
     1681 ggcttcgacc tgctggtgct ggacgtgttc atcgagaaca gcaacacgct ggacgatctg
     1741 gaggtctatc gcgaactggc cgccgacgcg ccgatcgcgg tgatgaccgc cgggcgcatc
     1801 gacaatcccg aagccggcgc cgacgcgctc aacaaggcgc gccgcgcgcg cgtcagcttc
     1861 ctgctgccca aaccgttctt tttcgtcgac gtcaagcagg tgtgcgaaga cgtcgaagcc
     1921 cactggagcc acgccgcccc gatcccgccg tccgccgcgc aacgcatagc cggttgaggc
     1981 gcgccgcatg agccacgctt ctctccggga cgctttcggc ctgtccgagg ccgatatctc
     2041 caacctcatg ggcctgatgc gccagagcga gacgctggtc gggctgatgg acgatttctt
     2101 cggcaagctg gacacgcgcc gctcgcagga actgctgaac atcgccgccg ccatcgccga
     2161 ggccaagacc gagctgcgcg ccctgcgccc gcgcggcctg cccagccagg gcctgaacgg
     2221 cgccaatcag gaactggacg ccatcgtgcg cgacgccgag gacgcgacca acgccatcct
     2281 gaacagcgcc gaggcgatcc tggagatgga cccggaaacc ggcggtctga aggacaaggt
     2341 cgacgatcag gtcatgcgca tcttcgaggc ctgctcgttt caggacatca tcggccagcg
     2401 cgtgtcgaaa atcgtcaccc tgctggccaa gatcgaaacc tgcctcggcg acatggccga
     2461 gcggctgggc gcgccggaac cggacgccgc gccgctgacg cctgaggacc ggcgccgccg
     2521 agacctgatg ctcaacggcc ccgccctgaa cggccccgaa gtcgcccagg acgccatcga
     2581 cgccctgttc gtctaggccg gatcgacatt caacgaccat acggcttcct gccgcctcct
     2641 acccattggc gagcaagggt ggccacagat gacgcaga
//
LOCUS       DASVLL010000595         4379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_201653,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000595 DASVLL010000000
VERSION     DASVLL010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201653"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /gene="def"
                     /locus_tag="VF402_09780"
     CDS             <1..336
                     /gene="def"
                     /locus_tag="VF402_09780"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7800628.1"
                     /translation="PEGADPDAEGVKNPRYFVNPEIVWASEELAPYEEGCLSVPEVYD
                     EVQRPARVRIRYLDYNGQPVEEEASGLYATCIQHEMDHLNGVLFIDHLSKLKRDRAVT
                     KVRKLRRAA"
     gene            397..1329
                     /gene="fmt"
                     /locus_tag="VF402_09785"
     CDS             397..1329
                     /gene="fmt"
                     /locus_tag="VF402_09785"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7800629.1"
                     /translation="MRLVFMGTPEFAVRALAELVAAGHEIVCVYSQPPAPKGRGQVLS
                     PSPVHAFAQSLGLEVRTPRSMKDLDAIAGFRALDIDAAVVVAYGQILKKEVLEHPPLG
                     CFNLHASLLPRWRGAAPIQRAIMAGDAFTGVEVMRMSEGLDEGPVLLSGRVEIAADDT
                     AQTLHDKLAALGASLLPVALAAIGRGGAQARPQTGEATYAKKITPDEARIDWTRPARD
                     LDFHIRGLSPFPGAWTTMDAGKGPQRLKVLMSRLSDETSGEVPGALLLGAGLKIATGD
                     GVIELTRVQREGKSAQDAVEFLKGSGLRPGDVLL"
     gene            1329..2108
                     /gene="truA"
                     /locus_tag="VF402_09790"
     CDS             1329..2108
                     /gene="truA"
                     /locus_tag="VF402_09790"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7800630.1"
                     /translation="MPRYKLWIEYDGRPYKGFQAQGGLPTVQGALEAAILKFSGQSLR
                     LTAAGRTDTGVHATGQVVSFDLDKPFPAATVRDAINACLQPAPIAVLRAVEADDDFSA
                     RFSATGRMYLYRILSRRAPPALDQGRVRHVKKPLDAAAMVEAARALIGTHDFTTFRHV
                     ECQAKSPVKTLDIARVARVGEEIHLVFAARSFLHRQVRSMTGTLVEAGLGRWTVADVA
                     AALAAKNRAACGQVAPAHGLYLTHVTYEAEPNFEKFSSIPR"
     gene            complement(2146..3318)
                     /gene="dapE"
                     /locus_tag="VF402_09795"
     CDS             complement(2146..3318)
                     /gene="dapE"
                     /locus_tag="VF402_09795"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX7800631.1"
                     /translation="MANTEISAKKSLDPIALAQALIARPSVTPADAGAMDTVQRALEG
                     LGFACTRLRFGDIENLYARRGTASPNLCFAGHTDVVPSGARDLWKYDPFGAEIHGGLL
                     IGRGAVDMKGAIAAWIAAVSRLDALPGSLSFLITGDEEGDAVDGTKKVVDYLKDKGEV
                     VDHCIVGEPTSVAHLGDMIKIGRRGSLNAVVTVTGRQGHVAYPDRARNPLPVLIDLLA
                     DLKGRVLDDGYEGFPPSNLEITSIDTGNPTTNLIPAQAAARLNIRFNPAHTGASLSAW
                     LTQRGEAHARESYRVEIKPRISGEAFLTEPGAFVDLILDTIRDRLGVAADPSTGGGTS
                     DARFIRDLCPVVEFGLVGQTMHQVNEAVGVADLQALTDAYQALIEAYFSKFEAKGR"
     gene            3393..>4379
                     /locus_tag="VF402_09800"
     CDS             3393..>4379
                     /locus_tag="VF402_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800632.1"
                     /translation="MTASQGGAADANGFDPDDNRHPLQIPNFRLLWLARMAAVLGGQA
                     QGAAIAWEVYTLARRHNGVNESAFYVGMFGLSQFIALFCLTIPAGIIADRVPRKTVMF
                     VALLLQSVLSLAFCAYSLFPDPPIWGLFVLGFVQGALRAFIAPATSALGPMLVPRQAL
                     SKAIAMNSMSMQVGSIVGPALGGMLIAISTQAAYVVCAIMLIVATALIFAIRADTRPV
                     GVTGSKTEMVKEGAEFIWKSKMLLGAMSLDLAAVLLGGATALIPVFSRDVLHVSPFYF
                     GLLQAATPAGAILVSMSLSQWPIRRRAGPWMYACVAIFGLATIVFGLSRNIWL"
BASE COUNT          656 a         1460 c         1494 g          769 t
ORIGIN      
        1 cccgaaggcg ccgacccgga cgccgaaggg gtgaaaaatc cgcgctattt cgtcaacccg
       61 gaaatcgtct gggcgtcgga ggaactggcg ccctacgaag aaggctgtct gtcggtgccg
      121 gaagtctatg acgaggtgca gcgtccggcg cgcgtgcgca tccgctatct cgactataac
      181 ggccagccgg tcgaggagga ggccagcggc ctctacgcca cctgcatcca gcacgagatg
      241 gaccacctga acggcgttct gttcatcgac cacctgtcca agctcaagcg cgaccgcgcg
      301 gtcaccaagg tcaggaagct gcgccgcgcg gcataaaggt tgcgcgatcc gcccaataat
      361 gattagacct cgcagccaag gttgcgaggt tttttcatgc gtctggtctt tatgggaacc
      421 cccgaattcg cggtgcgcgc gctggccgaa ctggtcgcgg cgggccatga gatcgtgtgc
      481 gtctattcgc agccgcccgc gccgaagggc cgggggcagg tcctatcgcc gtcgccggtc
      541 cacgccttcg cgcaaagcct gggccttgag gtgcgcacgc cccgatcgat gaaagacctg
      601 gacgccatcg ccggtttccg cgcgctcgac atcgacgcgg cggtggtcgt cgcctatggt
      661 cagatcctga agaaagaggt gctggagcat cccccgctcg gctgcttcaa cctgcacgcc
      721 tcgctgctgc cgcgctggcg cggcgcggcg ccgatccagc gcgccatcat ggcgggcgac
      781 gcctttaccg gcgtcgaggt catgcgcatg agcgaagggc tggacgaagg cccggttctc
      841 ctgtccggcc gcgtcgagat cgccgccgac gatacggcgc agaccctgca cgacaagctg
      901 gcggcgctgg gcgcgtccct gctgccggtc gccctggccg ccatcgggcg cggcggcgcg
      961 caggcgcgtc cgcagaccgg cgaggccacc tatgcgaaga agatcacgcc ggacgaggcg
     1021 cgcatcgact ggacccgccc ggcgcgcgat ctcgacttcc acattcgcgg cctgtcgccc
     1081 ttccccggcg cctggacgac gatggacgcg ggcaaggggc cgcagcgcct gaaagttctg
     1141 atgtcgcgcc tgagcgatga gacgtcgggc gaggttccgg gcgcgctgct gctgggcgcc
     1201 ggtttgaaga tcgccaccgg cgacggcgtg atcgagctga cgcgcgtgca gcgcgagggc
     1261 aagtcggcgc aggacgcggt ggaattcctc aagggttcgg gcctcaggcc cggcgacgtc
     1321 ctgctctgat gccgcgctac aagctttgga tcgaatatga cgggcggccc tataaggggt
     1381 ttcaggcgca gggcggcctg ccgaccgtgc agggcgcgct ggaggccgcc atcctgaaat
     1441 tcagcggcca gtccctgcgc ctgaccgccg ccgggcgcac cgacaccggg gtgcatgcga
     1501 cggggcaggt ggtcagtttc gacctcgaca agccgttccc cgccgccacg gtgcgcgacg
     1561 cgatcaacgc ctgccttcag ccggcgccga tcgcggttct gcgcgccgtt gaggccgatg
     1621 acgatttttc cgcgcgtttt tcggccaccg ggcgcatgta cctctaccgc atcctcagcc
     1681 gccgcgcgcc gcccgcgctc gatcagggcc gcgtccggca tgtcaagaag ccgctcgacg
     1741 cggcggcgat ggtcgaggcg gcgcgggcgc tgatcgggac gcacgacttc accaccttcc
     1801 gccatgtcga atgtcaggcg aaatcgccgg tcaagacgct cgacatcgca cgcgtcgcgc
     1861 gggtgggtga ggagattcat ctcgtcttcg ccgcgcgctc cttcctgcac cggcaggtgc
     1921 gttcgatgac cggcacgctg gtcgaggccg gtctggggcg ctggacggtc gcagatgtgg
     1981 cggcggcgct ggcggcgaag aaccgtgcgg cctgcggcca ggtcgcgccc gcgcacgggc
     2041 tgtacctgac ccacgtgacc tacgaagctg agccgaattt tgaaaaattc agctcgattc
     2101 ctcgttaaga caggcgtttt aacgcaaaag tgcgtcacac ttttgctacc gccctttggc
     2161 ttcgaacttc gaaaaatacg cctcgatcag cgcctgataa gcgtcggtga gcgcttgcaa
     2221 atccgcgacg ccgaccgctt cgttgacctg atgcatggtc tggccgacga ggccgaattc
     2281 gaccaccgga cacaggtcgc ggatgaagcg cgcgtccgag gtgccgccgc cggtcgacgg
     2341 atcggctgca acgccgagcc ggtcccggat cgtgtcgagg atcaggtcga cgaaagcgcc
     2401 cggctcggtc aggaaggcct cgccggaaat gcgcggcttg atctcgacgc ggtatgactc
     2461 gcgcgcgtgc gcctcgcccc gttgcgtcag ccacgcgctc agcgaggcgc cggtatgggc
     2521 cggattgaag cggatattga gacgcgctgc ggcctgcgcc gggatgagat tggtcgtcgg
     2581 attgccggtg tcgatgctgg tgatctccag attcgacggc ggaaagccct cataaccgtc
     2641 gtccagcacg cgccccttca ggtcggccag caggtcgatc agcacgggca gcggattgcg
     2701 cgcgcggtcg ggataggcga catggccctg ccgcccggtc acggtgacga cggcgttcag
     2761 gctgccgcgc cgcccgatct tgatcatgtc gccgagatgc gccaccgagg tcggctcgcc
     2821 gacgatgcag tggtcgacga cttcgccctt gtctttcagg tagtcgacga cctttttcgt
     2881 gccgtcgacc gcgtcgccct cctcgtcgcc ggtgatcagg aaggacaggc tgccgggcag
     2941 ggcgtcgagg cgcgacaccg ccgcgatcca cgccgcgatc gcgcccttca tgtcgaccgc
     3001 gccgcgcccg atcagcaggc cgccgtggat ctcggcgccg aacggatcgt acttccacag
     3061 gtcgcgcgcg ccggacggca ccacgtcggt atggcccgcg aagcacagat tgggggaggc
     3121 ggtcccgcgc cgcgcataca ggttctcgat gtcgccgaac ctgagccgcg tgcaggcgaa
     3181 gcccagccct tccagcgcgc gctgcaccgt gtccatcgcg cccgcgtcgg cgggcgtcac
     3241 cgacggacgg gcgatcagcg cctgcgccag cgctatcgga tcgagcgatt ttttcgcgga
     3301 aatctctgta ttcgccatgc gccctgctgt aagacccttt cctcgccggg ggaagtccgg
     3361 tttgtgtgtt tttttgctca aggttccctg acgtgaccgc atcgcaaggc ggcgccgccg
     3421 acgccaacgg gttcgacccc gacgacaatc gccacccgct gcaaatcccg aatttccgcc
     3481 tgttgtggct ggcgcgcatg gccgccgtgc tgggcgggca ggcgcagggc gcggccatcg
     3541 cctgggaggt ctatacgctg gcgcggcggc acaacggcgt caacgaatcg gccttctatg
     3601 tcggcatgtt cggcctgtcg cagttcatcg cgctgttctg cctgacgatt ccggcgggca
     3661 tcatcgccga ccgcgtgccg cgcaagacgg tgatgttcgt cgcgctgctg ctgcaatccg
     3721 tgctgtcgct ggcgttttgc gcctattcgc tgttcccgga tccgccgatc tggggcctgt
     3781 tcgtgctcgg cttcgttcag ggggcgttgc gcgccttcat cgcgccggcg acctcggcgc
     3841 tggggccgat gctggtgccg cgtcaggccc tgtcgaaggc catcgccatg aattcgatgt
     3901 cgatgcaggt cggctccatc gtcggtccgg cgctgggcgg gatgctgatc gcgatctcga
     3961 cccaggccgc ctatgtggtg tgcgcgatca tgctgatcgt ggcgaccgcg ctgatcttcg
     4021 ccatccgcgc cgacacccgg cccgtcggcg tcaccggctc caagaccgag atggtgaagg
     4081 agggggcgga attcatctgg aaatccaaga tgctgctggg ggcgatgtcg ctcgatctgg
     4141 cggcggtgct gctgggcggg gcgacggcgc tgatcccggt gttctcgcgc gatgtgctgc
     4201 acgtctcgcc cttctatttc gggctgttgc aggcggcgac gcccgcgggc gccatcctgg
     4261 tgtccatgtc gctgtcgcaa tggccgatcc gccgccgcgc cgggccgtgg atgtacgcct
     4321 gcgtggcgat cttcgggctg gcgaccatcg tgttcggcct gtcgcgcaat atctggctg
//
LOCUS       DASVLL010000596         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_525943,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000596 DASVLL010000000
VERSION     DASVLL010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525943"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VF402_09805"
     CDS             <1..1343
                     /locus_tag="VF402_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEX7800633.1"
                     /translation="PGGNYLSDWDWHDAIGPRDKRPPMYDHAWDAMQTNDIGMDEWME
                     LCRLIGTMPYVTVNAGLGDANSAAEEVEYLNGPASSYWGGLRAKYGHPAPYGIKYWDI
                     GNEPYGDWQIGKTTLQYYAYKHIDFARRMKKADPSITLLASGAMPDQRKWPGVEKENA
                     NTQSIIPKFGTELDWTGGMLAKASDYFDGVTEHWYDTAEKRPDAPPDDELIEFARQPS
                     NNVLMKAKEWDIYRQKYPVIDKKDMFLSIDEYAYFPSAPDLKTSLAYSMVLQEMLRHT
                     DAIRMSAFTMGPATLDQTADASTLNVTGLVFKLYGAHFGAGVTPVSVTGDTPWPDPKY
                     PVGLDHPQVVAGSPTWPVDVVAALSADRKKLIVGAVNATYEPRELALNLGKLAVAANG
                     TSWRLAGASLDAVNKAGQPPAVTIRSEAAALNGGKLSVPAATTIIYEFDITGGL"
     gene            1373..2455
                     /locus_tag="VF402_09810"
     CDS             1373..2455
                     /locus_tag="VF402_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7800634.1"
                     /translation="MKAQLILGGLLAAALTLTAPAVQAQVQTLVPPVVPGARPVIVQT
                     IVVHSKALEGNLEGESPDRKVIVYLPPDYDKNPNKRYPVVYALHGYSIDNEMWTHEIH
                     TPQTIEGAFATGSREMIFVLPDGRTLHNGSMYSNSPTVGNFEGFIAHDLVAYIDSHYR
                     TLANRDSRGLAGHSMGGYGATRIGMKYPEVFGALYIMSPCCLSARGAPDADMARKLEA
                     VTTKEEAVKLDWFSRATMASAAAWSPDPKKPPLYLDLPTKDGVPQPDVLAKWAANAPL
                     AMVDQYISNLKQYNAIAIDVGDQDGLNADTKKLHEALDSYGVANSFEIYPGTHTSAVA
                     DRFQNHVVPFFSQHLKFEAEKQAPKK"
     gene            2639..>2905
                     /locus_tag="VF402_09815"
     CDS             2639..>2905
                     /locus_tag="VF402_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7800635.1"
                     /translation="MNAENDLYEGLRAELRRGALSLAVLARLRVERYGYSLRQALAED
                     GLDMEESTLYPLLRRLETQGLLVSEWREEDKRRKRFYRLSAAGET"
BASE COUNT          576 a          974 c          906 g          449 t
ORIGIN      
        1 tgccgggcgg caactacctg tccgactggg actggcatga cgccatcggc ccgcgcgaca
       61 aacgcccgcc gatgtacgac cacgcctggg acgcgatgca gaccaacgac atcggcatgg
      121 acgaatggat ggagctgtgc cgcctgatcg ggaccatgcc ctatgtcacg gtcaacgccg
      181 gtttaggcga cgccaattcc gccgccgagg aggtcgaata tctgaacggc ccggccagtt
      241 cctactgggg cggcctgcgc gcgaaatacg gccacccggc gccttacgga atcaagtact
      301 gggacatcgg caacgagcct tacggcgact ggcagatcgg caagacgacg ctgcaatatt
      361 acgcctataa gcatatcgac ttcgcgcgcc gcatgaagaa ggccgatccc tcgatcaccc
      421 tgctggcctc cggcgccatg ccggaccagc gcaaatggcc gggggtcgag aaggaaaacg
      481 ccaacacgca gagcatcatc cccaagttcg gcaccgagct ggactggacc ggcggcatgc
      541 tggccaaggc gtcggactat ttcgacggcg tgaccgagca ttggtacgac acggcggaaa
      601 agcgtcccga cgccccgccc gacgacgagt tgatcgagtt cgcgcgccag ccgtccaaca
      661 acgtgctgat gaaggcgaag gaatgggaca tctaccgcca gaaatatccg gtgatcgaca
      721 agaaggacat gttcctgtcg atcgacgaat acgcctactt cccatccgcg cccgacctga
      781 agacctcgct ggcctattcg atggtgttgc aggaaatgct gcgccatacc gacgccatcc
      841 gcatgtcggc cttcaccatg ggaccggcga cgctggacca gacggcggac gcctcgacgc
      901 tcaacgtgac cggactggtg ttcaagctgt acggcgcgca tttcggcgcg ggcgtcacgc
      961 cggtgtcggt gacgggcgac acgccgtggc ccgatccgaa atatccggtc gggctggacc
     1021 atccgcaggt cgtggcgggc agcccgacct ggccggtcga tgtcgtcgcc gccctcagcg
     1081 ccgacaggaa gaagctgatc gtcggcgcgg tcaacgccac ctatgagccg cgcgagctgg
     1141 cgctgaacct cggcaagctg gccgtggcgg cgaacgggac gtcgtggcgg ctggcgggcg
     1201 cgtcgctcga cgcggtcaac aaggcgggcc agccccccgc cgtgaccatc cgctccgaag
     1261 ccgccgcgct gaacggcggc aagctgagcg tgcccgccgc gacgacgatc atctacgaat
     1321 tcgacattac gggcggcctc tgaacgcggc cccagcaggg agacatttta taatgaaagc
     1381 acagcttatc ctcggcgggc ttctggcggc ggcgctgacg ctgaccgccc ccgccgttca
     1441 ggcgcaggtc cagaccctgg tgccgccggt cgttccgggc gccaggcccg tgatcgtgca
     1501 gaccatcgtc gtccactcca aggcgctgga aggcaatctc gaaggcgaat cccccgaccg
     1561 caaggtgatc gtctatctgc cgcccgacta cgacaagaac ccgaacaagc gctatccggt
     1621 cgtctatgcg ctgcacggct attccatcga caacgagatg tggacgcacg aaatccacac
     1681 gccgcagacc atagagggcg ccttcgccac cggcagccgc gagatgatct tcgtcctgcc
     1741 cgacggacgc accctgcaca acggctcgat gtattcgaac tcgcccacgg tcggtaattt
     1801 cgagggcttc atcgcccacg acctcgtcgc ctatatcgac agccattacc gcaccctcgc
     1861 caaccgcgac agccggggtc tggccggtca ctcgatgggc ggctacggcg ccacgcgcat
     1921 cggcatgaaa tatcccgaag tgttcggcgc gctctacatc atgagcccgt gctgcctgtc
     1981 cgcgcgcggc gccccggacg ccgacatggc caggaagctc gaagccgtca cgacgaagga
     2041 ggaggccgtc aagctcgact ggttctcgcg cgccacgatg gccagcgccg ccgcctggtc
     2101 gcccgacccg aagaagccgc cgctctatct cgacctgccg accaaggacg gcgtgccgca
     2161 gcccgacgtg ctggccaaat gggcggccaa cgcgccgctg gccatggtcg atcagtacat
     2221 ctccaacctg aagcagtaca acgccatcgc gatagatgtc ggcgatcagg acgggctgaa
     2281 tgccgatacg aaaaagctgc atgaggcgct cgattcctac ggcgtcgcca acagcttcga
     2341 aatctacccc ggcacccaca ccagcgccgt ggccgaccgc ttccagaacc acgtcgtccc
     2401 cttcttcagc cagcacctga agttcgaggc cgaaaaacag gccccgaaaa aatagagcgt
     2461 ttccccaaaa agcggttccc ctttttggat aaggccacgc gatgtttcgg ctccggccgt
     2521 ctcccggcga gacggccgga gccggacgcg cgtcctgacc ccgtcttgac agggcctgct
     2581 gttttgcaca tactgtatga tatacagtgt atgaggcaca ctatgcgaca cgcggcacat
     2641 gaacgctgag aacgacctct atgagggcct gcgcgccgaa ttgcggcgcg gcgcgctgag
     2701 tctggcggtg ctggcgcgcc tgcgggtcga gcgttacggc tactcgctgc gtcaggcgct
     2761 ggcggaggac gggctggata tggaggaaag cacgctctac ccgctgctgc gccgcctgga
     2821 gacgcagggc ctgctggtca gcgagtggcg cgaggaggac aagcgcagga agcgttttta
     2881 ccggctgtcg gcggcgggcg aaacc
//
LOCUS       DASVLL010000597         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_416254,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000597 DASVLL010000000
VERSION     DASVLL010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416254"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..818)
                     /locus_tag="VF402_09820"
     CDS             complement(60..818)
                     /locus_tag="VF402_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7800636.1"
                     /translation="MRSFAFALAYWVLSIVYSLLALILSLLPGRAPVRWVIRRYTRRM
                     VQAMRVFAGIRLEVRGREHLLDRPVIYACKHQSWGDGFCIYAQFDDLAFVTGDHLEKY
                     PLMGTLLRKLGAIVVNNCGGHQARKALASRAADAAREGRSILIYPEGHLNAPGTYRRY
                     RAGVWHMMRDFDMPVVPVATNLGLFWQEQSFRKRPGTAVLEFLEPIMPGMDKDAFLET
                     LRDRIETRSQRLISEATGLPFTPSLPADVMTQTG"
     gene            complement(883..>1917)
                     /locus_tag="VF402_09825"
     CDS             complement(883..>1917)
                     /locus_tag="VF402_09825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7800637.1"
                     /translation="IRSPNAYALAGEAMRLMQEHGVWPTPLNYELWLYVAGDPECALA
                     QEIFRLVAAGETITEDVSESLASKFIARLKLNDEVRDAGLKLTRELASITEVIADAQK
                     TQRTYSETLQNARRTMAEVDVSALQKLIDNLSHATTTVLDHHLSLESRLSESSREVNR
                     LRKHLDQVRMEAMTDALTNLANRKSFDEALDNHCDKDDVSTPLSLAVIDIDHFKRFND
                     TWGHQTGDQVLRYVASVLARIGRPPRMAARYGGEEFGLLFPGETSAQVERLLNEARQE
                     IASRVLKRRSTNEDLGTVTISVGVAQRQYGEDSFDLLDRADKALYHSKNAGRNRVSVA
                     ETLEAGSNAA"
BASE COUNT          320 a          587 c          655 g          355 t
ORIGIN      
        1 tgacggctct ccccaactct cggcttcaag gttaggggca tgaaccttaa taaagacttt
       61 tatcccgtct gcgtcataac gtcggcgggc agggacggcg tgaagggcag gcccgtcgcc
      121 tcgctgatca ggcgctggct gcgcgtttcg atgcggtcgc gcaaggtctc cagaaaggcg
      181 tccttgtcca tgccgggcat gatcggctcc aggaactcca gcacggcggt tccggggcgt
      241 ttgcggaagc tttgctcctg ccagaacagg cccagattgg tggccaccgg cacgaccggc
      301 atgtcgaagt cgcgcatcat gtgccacacc ccggcgcggt agcggcgata ggttccgggc
      361 gcattgaggt ggccttcggg atagatcagg atcgagcggc cttcacgggc ggcgtcggcg
      421 gcgcggctgg ccagggcctt gcgcgcctgg tggccgccgc agttgttgac cacgatggcc
      481 cccagcttgc gcagcagggt tcccatcagc ggatattttt cgagatggtc gccggtgacg
      541 aaggccagat cgtcgaactg ggcgtagatg cagaagccgt cgccccagct ctggtgcttg
      601 cacgcataga tgacgggacg gtccagcagg tgttcgcggc cgcgcacctc aagccggatg
      661 cccgcgaaga cgcgcatcgc ctgcaccatg cggcgcgtat agcgccggat gacccagcgc
      721 accggcgccc ggcccggcag cagggacagg atcagcgcca gcaggctata gacgatggac
      781 aagacccagt aggcgagggc gaaggcgaaa ctgcgcatgg acgatctccc ggacaatgaa
      841 tttgaacatt gttcaacttc attgtcaagg gaaaggcgcg gatcaggcgg cgttggagcc
      901 ggcttccagc gtttcggcca cgctgacgcg gttgcgcccg gcgtttttgg agtggtacag
      961 cgccttgtcg gcgcggtcga gcaggtcgaa ggaatcctcg ccgtactgac gctgcgcgac
     1021 gccgaccgag atggtcacgg tgccgagatc ctcgttggtc gagcggcgtt tcagcacgcg
     1081 cgaggcgatt tcctgccgcg cctcgttgag caggcgctcg acctgggcgc tggtctcgcc
     1141 ggggaacagc aggccgaact cctcgccgcc gtagcgggcg gccatgcgcg gcggacggcc
     1201 gatgcgcgcc agcaccgagg cgacgtagcg cagcacctga tcgccggtct gatgacccca
     1261 ggtgtcattg aagcgcttga aatggtcgat gtcgatcacg gccaggctga gcggcgtgga
     1321 gacgtcgtcc ttgtcgcagt gattgtcgag cgcttcgtcg aacgacttgc ggttggccag
     1381 attggtcagg gcgtcggtca tcgcctccat gcgcacctga tcgaggtgct tgcgcaggcg
     1441 gttgacctcg cgcgacgatt cggacaggcg gctttccagc gacagatggt gatccagcac
     1501 cgtcgtggtc gcgtggctca gattgtcgat cagcttttgc agggccgaga cgtcgacctc
     1561 ggccatcgtc ctgcgcgcgt tttgcagggt ttcggaatag gtgcgctgcg tcttctgcgc
     1621 gtcggcgatc acctcggtga tcgaggccag ttcgcgggtc agtttcaggc cggcgtcgcg
     1681 cacctcgtcg ttcagcttga ggcgggcgat gaatttcgag gccaggcttt ccgacacgtc
     1741 ttccgtaatc gtctctccgg cggcgaccag acggaaaatc tcctgcgcca gcgcgcattc
     1801 agggtcgccg gcgacgtaca gccacaactc ataattcaac ggggtcggcc agacgccatg
     1861 ctcctgcatc agccgcatcg cttcgccggc cagggcgtag gcgttcgggc tgcggat
//
LOCUS       DASVLL010000598         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_94668, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000598 DASVLL010000000
VERSION     DASVLL010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94668"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..649
                     /locus_tag="VF402_09830"
     CDS             221..649
                     /locus_tag="VF402_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HEX7800638.1"
                     /translation="MTIRINSPLGAAQAGAGGAVRKGGGEGFSLSGASGASKSAGPAQ
                     ASAAVGMLGVDALLAIQSEDDVMTGRRRRQVRRSKDILDALDDLRISVLSGDIDDEAL
                     LRLQGLIALHREDVADDRLQGVLNEIETRACVELAKRRLI"
     gene            847..1275
                     /gene="dksA"
                     /locus_tag="VF402_09835"
     CDS             847..1275
                     /gene="dksA"
                     /locus_tag="VF402_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX7800639.1"
                     /translation="MTALDLPELNAAYRPSDDEEYMSPRQLAYFRAKLNAWKDEILQG
                     ARETVNIMQKETENHPDLVDRASSESDRALELRTRDRQRKLIAKIDEALSRIDDGSYG
                     FCEETGEPIGLGRLEARPTATLSIEAQERHERSERVHRDD"
     gene            complement(1325..>1522)
                     /locus_tag="VF402_09840"
     CDS             complement(1325..>1522)
                     /locus_tag="VF402_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX7800640.1"
                     /translation="ISSLSALVAAALVVPVSLLLPFVDPAYPFVGMALFMAVLIYIRH
                     RANIVRLLKGEEPKIGKKKEA"
BASE COUNT          299 a          470 c          505 g          248 t
ORIGIN      
        1 aattcagtaa atacagctga ttaattccgc gtcatcgcaa agccttaacg agatggaaaa
       61 aattgccggg ccgttaaggc ttcgcttacc caggatgggc aattatggcc gggtcatcgc
      121 cgccggtaaa aagccggtct gtccggcaac ggacggctca ggaagaattt taagcgatct
      181 tacaaagtct ttcgacatgg tttccggtgt aggctccagc atgacgatca ggatcaattc
      241 gcccttgggg gccgcgcagg cgggcgcggg cggcgccgtg cgcaaaggcg gcggcgaggg
      301 cttttcgctg tcgggcgcgt cgggcgcctc gaagtccgcc ggtccggcgc aggcgtccgc
      361 cgcggtcggc atgctgggcg tggacgcgct tttggccatc cagtccgagg acgacgtgat
      421 gaccgggcgc cgccgccggc aggtcaggcg ctcgaaggac attctcgacg cgctggacga
      481 cctgcgcatc agcgtgctgt ccggcgatat cgacgacgaa gcgctgctgc gcctgcaagg
      541 gctgatcgcg ctgcaccgcg aggacgtggc cgacgaccgg ctgcaagggg tgctgaacga
      601 aatcgagacg cgcgcctgtg tcgaactggc caaacgccgc ctgatctgat ccgtttcgcg
      661 acagtccggc ggcaggaaaa aagcttcaca gcccttgacg gacactctat aagccctgcc
      721 atcgcgcggc tggcgactcg tttggcggag ggctgtggtc gcgtggcgtc gaacttgccg
      781 tgcgccgggc gtgatcgccg gtgcggcgtt ttcggataaa gccatatcag gggtgtgaag
      841 agacacatga cagccttaga cctgccggag ttgaacgccg cgtaccgtcc ctcggacgac
      901 gaagagtata tgagcccgcg ccaactggcc tatttccgcg ccaagttgaa cgcctggaag
      961 gacgagatcc ttcagggcgc gcgcgagacg gtcaacatca tgcagaaaga gaccgagaat
     1021 catcccgacc tggtggaccg cgcctcgtcg gaatccgacc gcgcgctgga gctgcgcacc
     1081 cgcgaccgcc agcgcaagct gatcgccaag atcgacgagg ccctgtcgcg catcgacgac
     1141 ggctcctacg gcttctgtga ggaaacgggc gagccgatcg gccttggccg tctcgaagcc
     1201 cgcccgaccg cgaccctgtc catcgaggcg caggaacggc acgaacgctc cgaacgggtc
     1261 caccgcgacg actaaaagac aaggcccgcc ggaaccggcg ggccttgtct ttcgacaggg
     1321 gggcttacgc ttctttcttc ttcccgatct tcggctcctc gcccttcaac aggcggacga
     1381 tattggcgcg gtggcggatg tagatcagca cggccatgaa cagggccatg ccgacgaacg
     1441 gataggccgg atcgacgaac ggcagcagca ggctgaccgg cacgacgagg gcggcggcga
     1501 ccagcgcgct cagcgacgag at
//
LOCUS       DASVLL010000599         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_362900,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000599 DASVLL010000000
VERSION     DASVLL010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362900"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..590
                     /gene="tatB"
                     /locus_tag="VF402_09845"
     CDS             21..590
                     /gene="tatB"
                     /locus_tag="VF402_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX7800641.1"
                     /translation="MVPGIGGGELVVIAVVALIVVGPKDLPKLLRQLGRMVGKMRRMA
                     DEFRTSFEDMARQSELDELRKEVDALRSGQMQPSIVSDINQQMRAIESDINAPSAAAA
                     APVAPVEVADMPPAEPPVAEAAPISETARDNPQLDGKPPRKRAAPKTAPDPAPDLATE
                     PAAKTPRVRKAASKAKPAQPKRARKKAES"
     gene            587..1510
                     /gene="tatC"
                     /locus_tag="VF402_09850"
     CDS             587..1510
                     /gene="tatC"
                     /locus_tag="VF402_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7800642.1"
                     /translation="MSDETLPQTVDDAGKRSLIGPIVDHDEAEIEASRAPLMDHLVEL
                     RSRLIKMVVAFFVCVIGCYAFHNHIMKFLVHPLAVAASLYQEQQHGHKSGPFDLMFVL
                     TGIKQVANVKDIPLIATAPMEVFFTNLRIAMFGGVIVSFPVLAYQVYRFVGPGLYKRE
                     RMAFLPFLFAAPVLFVLGMCLVYFAILPMVLWFSLNQQIVSGAGVMVQFMPKVSEFLE
                     LVETLMIAFGLCFQLPIVITLLGMTGIVTAKMLGSFRRYAILAIVVVAAIVTPPDPIS
                     QCLLSIPIVLLYEVSILCVKLVEFNRRHRKA"
     gene            1666..2949
                     /gene="serS"
                     /locus_tag="VF402_09855"
     CDS             1666..2949
                     /gene="serS"
                     /locus_tag="VF402_09855"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7800643.1"
                     /translation="MHDIKALRETPDLFIEAWTRRGRDSASKDAHLAVSLDGELRAAQ
                     TRFQTAQAELKKLSAQIGQAKAQKDEARAQTLMAEVEGLKGAIAEAQETERAKAEELR
                     NHLAALPNLPFDDVPVGADEHGNVEVRRHGAPAALGFAAKDHADLGEALGLMDFEAAA
                     KISGARFVVLKGQLARLERAIGQFMLDVQTQEHGYTEVNPPVLVRGEALYGTGQLPKF
                     EEDLFKTTDDRYLIPTAEVSLTNLVREAITAEEELPLRLTALTNCFRAEAGSAGRDTK
                     GMIRQHQFQKVELVSITTPEQSADEHERMTACAENILKKLDLSFRTMLLCTGDMGFGA
                     KKTYDLEVWLPSQNTYREISSCSNCGDFQARRMDARTRKAGEKGTRFVHTLNGSGLAV
                     GRTLVAIMENYQDEGGRIAVPSALVPYMGGLTHIG"
BASE COUNT          591 a         1010 c         1071 g          550 t
ORIGIN      
        1 cttagagaaa gcgtaagatc atggtgcccg gtatcggggg cggagaactg gtcgtcatcg
       61 cggtggtggc cctgatcgtg gtcgggccga aagacctgcc gaagctgttg cggcaactgg
      121 gccgcatggt gggcaagatg cgccgcatgg ccgacgaatt ccgcacctcc ttcgaggata
      181 tggcgcggca gagcgagctg gacgaactgc gcaaggaggt cgacgccctg cgttccggcc
      241 agatgcagcc gtccatcgtc agcgacatca atcagcagat gcgcgccatc gaaagcgaca
      301 tcaacgcgcc gtccgccgcc gccgcggctc ccgtcgcgcc ggtcgaggtc gcggacatgc
      361 cgcccgccga gccgccggtt gccgaagccg cgccgatttc cgaaacggcc agggacaatc
      421 cgcaactgga cggcaagccg ccgcgcaagc gcgccgcgcc gaagacggcg cccgacccgg
      481 cgcccgacct ggcgactgaa ccggcggcga aaacgccgcg cgtccggaaa gcggcgtcca
      541 aagccaaacc tgcccaaccg aaacgcgcgc gcaagaaggc ggagtcatga gcgacgaaac
      601 cctgccgcaa accgtcgacg acgcgggcaa gcgcagcctg atcggtccga tcgtcgatca
      661 tgacgaggcc gagatcgagg cgtcgcgcgc gccgttgatg gaccacctgg tcgagttgcg
      721 ctcgcgcctg atcaagatgg tggtcgcttt cttcgtctgc gtgatcggct gctacgcctt
      781 ccacaaccac atcatgaaat tccttgtgca tccgctggcc gtggccgcgt cgctgtatca
      841 ggagcagcag cacggccaca agtcggggcc gttcgacctg atgttcgtgc tgaccgggat
      901 caagcaggtc gccaatgtga aggacattcc gctgatcgcc acggcgccga tggaggtgtt
      961 cttcacaaac ctgcgcatcg ccatgttcgg cggcgtgatc gtcagctttc cggtgctggc
     1021 ctatcaggtt taccgcttcg tcggaccggg gctttacaag cgcgagcgca tggccttcct
     1081 gccgttcctg ttcgcggcgc cggtgctgtt cgtgctgggc atgtgcctgg tctatttcgc
     1141 catcctgccg atggtgctgt ggttctcgct gaaccagcag atcgtcagcg gcgccggggt
     1201 gatggtccag ttcatgccca aggtgtcgga gttcctcgaa ctggtcgaga cgctgatgat
     1261 cgccttcggc ctgtgctttc agttgccgat cgtgatcacc ctgctgggca tgaccggcat
     1321 cgtgacggcg aagatgctcg gctcgttccg gcgctacgcc atcctcgcca tcgtcgtggt
     1381 cgccgccatc gtcacgccgc ccgatccgat ctcgcaatgc ctgttgtcga tcccgatcgt
     1441 gctgctgtac gaggtgtcga tcctgtgcgt gaaactggtc gaatttaacc gacgccaccg
     1501 taaggcctga gccttgcctg acaaggcgca atccgtgtaa gaggctgcat cccttatacc
     1561 tccctgaacg cgaagcgttt ggggaggggg accgcacgag gcccgaatgt aacgaggggc
     1621 gagatgtggt ggtggggttt ctgacttatc tccaaggctg acaagatgca cgatatcaag
     1681 gctctgcgcg aaactcccga cctgttcatc gaagcgtgga cgcggcgcgg tcgcgactcc
     1741 gcctcaaagg acgcgcatct ggccgtcagc ctcgacggcg aactgcgcgc ggcgcagacg
     1801 aggttccaga ccgcgcaggc cgagctgaaa aagctgtcgg cgcagatcgg ccaggccaag
     1861 gcgcagaagg acgaggcgcg ggcgcaaacc ctgatggccg aggtcgaggg gctgaaaggc
     1921 gcgatcgccg aggcgcagga aaccgagcgc gccaaggccg aggaattgcg caaccatctg
     1981 gccgccctgc cgaacctgcc gttcgacgac gtgccggtcg gggcggacga acacggcaat
     2041 gtcgaggtcc gccgtcacgg cgcgccggcg gcgcttggct ttgcggccaa ggaccacgcc
     2101 gatctcggcg aggcgctcgg cctgatggat ttcgaggcgg cggcgaagat ttccggcgcg
     2161 cgtttcgtgg tgctgaaagg gcaactggcg cggctggagc gcgccatcgg ccagttcatg
     2221 ctcgacgtgc agacgcagga gcatggctac accgaagtca atccgccggt gctggtgcgc
     2281 ggcgaggcgc tctacggcac cgggcagttg ccgaagttcg aggaagacct gttcaagacc
     2341 acggacgacc gctacctgat ccccaccgcc gaggtgtcgc tgaccaacct cgtccgcgag
     2401 gcgatcacgg cggaggagga actgccctta cgcctgaccg ccctgaccaa ctgtttccgc
     2461 gccgaggccg ggtcggcggg gcgcgacacc aaggggatga tccgccagca tcagttccag
     2521 aaggtcgaac tggtgtcgat caccacgccg gagcagtcgg cggacgagca tgagcgcatg
     2581 acggcgtgcg ccgaaaacat cctgaaaaag ctcgatctgt cgttccgcac catgctgctc
     2641 tgcaccggcg acatgggctt cggcgccaag aagacctacg atctcgaagt ctggctgccg
     2701 tcgcagaaca cttatcgcga gatttcgtcc tgctcgaact gcggcgactt tcaggcgcgg
     2761 cgcatggatg cgcgcacacg caaggcgggc gagaagggga cgcgcttcgt gcatacgctg
     2821 aacgggtcgg gactggcggt cggtcggact ctggtggcga tcatggagaa ttatcaggac
     2881 gagggcggcc gcatcgccgt gccgtccgcg ctggtcccct acatgggcgg cctgacgcat
     2941 atcgggtgag gttctcctcc ccacctgtgg ggaggtggcg tttgcgcagc aaacgacgga
     3001 ggggtggctt gccgcccgcg ccgttacccc tccaccgctt cgcggtcccc ctccccataa
     3061 aatggggagg agaaagaaca ccacaggaat tttgctttcg cgcggttgcc ttggcggcgg
     3121 aaaaggccgt acaagtcgtg aacattccgc acggtgagac tgtatgcgta tcctgctgac
     3181 caatgacgac ggcgtcgagg cttacgggct gaaggtgctt ta
//
LOCUS       DASVLL010000600         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_362959,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000600 DASVLL010000000
VERSION     DASVLL010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362959"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VF402_09860"
     CDS             <1..235
                     /locus_tag="VF402_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron dicitrate transport regulator FecR"
                     /protein_id="HEX7800644.1"
                     /translation="AVRDVAAEFAGRGAQVCLANPESPIAGTIALPALSAHPALEPIL
                     LIESFYRLANSLSLARGLNPDAPPHLNKVTKTV"
     gene            232..1359
                     /gene="nagA"
                     /locus_tag="VF402_09865"
     CDS             232..1359
                     /gene="nagA"
                     /locus_tag="VF402_09865"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX7800645.1"
                     /translation="MSQITFINGRLVTPQGVVEGALTATGETITAIDGEPEGEIVDLD
                     GGWLLPGFIDTQVNGGGGVLFNDDTTADGIAAIGRAHRAYGTTGFLPTLISDELSVIS
                     AAMRAVEDAIAAGVPGVLGIHIEGPFISKQRKGIHNPDLFRTLDAESKALLKSLKRGR
                     TLVTLAPENCAPDDIRELAGAGVIVAAGHTDATYAETKQALAAGVTGFTHLFNAMSPM
                     LHRAPGVVGAALENQSAYCGLIADGHHVDWPVLAVALRTRPHDRFMLVTDAMPTVGSP
                     AKTFTLNGQPIHVENGVCVGPDGTLAGSDLDMITAVRNMVEHVGVPIGDAALMAAEAP
                     ARFLGLDAARGSLSLGRRADFVWLDDKLNLKATFIGGRQGH"
     gene            complement(1373..1717)
                     /locus_tag="VF402_09870"
     CDS             complement(1373..1717)
                     /locus_tag="VF402_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800646.1"
                     /translation="MQTTTLWRPVGPQELALIEETGMAAFPPRLPEQPIFYPVTTREY
                     AIKIARDWNVPDSGAGYVTQFEVRSDFLAHYRVETAGGRAHQEYWIPAEDLDAFNQAI
                     IGPILITDTFTR"
     gene            complement(1717..2109)
                     /locus_tag="VF402_09875"
     CDS             complement(1717..2109)
                     /locus_tag="VF402_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX7800647.1"
                     /translation="MHDDTHIRSLRGKDGLQVVEGDRIDEDVERRVAERTEAEFARLE
                     LVPPAQKAAREEKRVRELPINADTVKLRNELALLKQEHKDLDDSISALEIVPQPDQIL
                     IARLKRKKLALRDQISKIEDRIRPDIIA"
     gene            complement(2180..>2374)
                     /locus_tag="VF402_09880"
     CDS             complement(2180..>2374)
                     /locus_tag="VF402_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800648.1"
                     /translation="AELQAEKALMARLAALDDGKSTLNQDVAEALRATAAAWGGTAPA
                     EALSRLSQALTKGGFLGYTD"
BASE COUNT          392 a          836 c          755 g          391 t
ORIGIN      
        1 cgcggtgcgc gacgtcgccg ccgaattcgc cgggcgcggc gcgcaggtct gcctggccaa
       61 tcccgaaagc ccgatcgccg ggacgatcgc cctgcccgcc ctgagcgcgc atccggcgct
      121 ggagccgatc ctgctgatcg agagcttcta ccgcctcgcc aacagcctgt cgctggcgcg
      181 cggcctcaat cccgacgcgc cgccccacct caacaaggtg acgaaaaccg tatgagccag
      241 atcaccttca tcaacggccg cctcgtcacg ccgcagggcg tggtcgaagg cgccctgacc
      301 gcaaccggcg agacgatcac cgccatcgac ggcgagcccg aaggcgaaat cgtcgatctc
      361 gacggcggct ggctgctgcc cggcttcatc gacacccagg tcaacggcgg cggcggcgtg
      421 ctgttcaacg acgacacgac ggcggacggc atagcggcca tcgggcgcgc gcaccgcgcc
      481 tacggcacga ccggtttcct gccgaccctg atcagcgacg agctgagcgt gatcagtgcg
      541 gccatgcgcg cggtcgagga cgccatcgcg gcgggcgtgc cgggcgtgct gggcatccat
      601 atcgaaggcc cgttcatctc gaagcagcgc aagggcatcc acaatcccga cctgttccgc
      661 acgctggacg ccgagtccaa ggcgctgctc aagagcctga aacgcggcag gacgctggtg
      721 accctggcgc cggagaactg cgcgccggac gacattcgcg aactggccgg ggcgggcgtg
      781 atcgtcgcgg cgggccacac cgacgcgacc tatgccgaga cgaaacaggc gctggcggcg
      841 ggcgtgaccg gcttcaccca cctgttcaac gccatgtcgc cgatgctgca ccgcgcgccc
      901 ggcgtggtcg gcgccgcgct ggagaaccag agcgcctact gcggcctgat cgcggacggc
      961 catcatgtcg actggccggt gctggccgtc gcgctgcgca cgcgcccgca cgaccgcttc
     1021 atgctggtca ccgacgccat gccgacggtc ggctctccgg ccaagacctt cacgctcaac
     1081 ggccagccga tccatgtcga gaacggcgtc tgcgtcggcc cggacggcac gctggccggg
     1141 tcggacctcg acatgatcac ggcggtgcgc aacatggtcg aacatgtcgg cgtcccgatc
     1201 ggggacgccg ccctgatggc cgccgaagcg cccgcgcgct tcctcggcct cgacgccgcg
     1261 cgcggatcgc tcagcctcgg tcgccgcgcc gacttcgtct ggctggacga caaactgaac
     1321 ctgaaagcga cctttatcgg cggccggcag ggtcattgac cctgccccgg tcctagcgcg
     1381 taaatgtatc cgttataagg atcgggccaa taatggcctg attgaaagcg tccaggtctt
     1441 cggcaggaat ccagtattcc tgatgcgcgc ggccgcccgc tgtttcgacg cgatagtgcg
     1501 cgaggaagtc tgaccgcacc tcaaactgag tgacataacc tgcgccgctg tccggcacat
     1561 tccagtcgcg cgcgatcttg atcgcatact cgcgggtcgt caccgggtag aagatcggct
     1621 gttccggcag acgcggcgga aacgccgcca tgccggtctc ctcgatgagc gccagttcct
     1681 gcggtccgac aggccgccaa agagtcgtgg tctgcatcag gcgatgatat ccggcctgat
     1741 ccgatcctcg atcttggaga tctgatcgcg cagggccagc ttcttgcgct tgaggcgggc
     1801 gatcaggatc tggtccggct gcggcacgat ctccagcgcc gagatggagt cgtcgagatc
     1861 cttatgctcc tgcttgagca gggccagttc gttgcgcaat ttcaccgtat ccgcgttgat
     1921 cggcagttcg cgcacgcgct tttcctcccg cgcggccttc tgcgcgggcg ggaccagttc
     1981 gaggcgcgcg aattcggctt ccgtgcgctc cgccacgcgg cgctcgacgt cttcgtcgat
     2041 cctgtcgccc tcgaccacct gcaaaccgtc cttgccgcgc agactgcgga tatgggtgtc
     2101 gtcgtgcatg ggcggccccg gcctttctgt taaaggattg aagcttttgg acaaacggcg
     2161 cgccaacgcc gccggatcat cagtcagtat agcccagaaa accgcccttg gtaagagcct
     2221 gcgacaatcg cgacagcgcc tcggcgggcg ccgttccgcc ccacgccgcc gccgtggcgc
     2281 gcaaagcctc ggcaacatcc tgattcaacg ttgattttcc gtcgtcgagc gccgccagcc
     2341 gcgccatcag cgccttttcg gcctgcaatt ccgc
//
LOCUS       DASVLL010000601         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_416602,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000601 DASVLL010000000
VERSION     DASVLL010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416602"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2372
                     /locus_tag="VF402_09885"
     CDS             <1..>2372
                     /locus_tag="VF402_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7800649.1"
                     /translation="HSFRILRGDQVIDVLAHQGFTVVRRETNLDRAMLDGLLTGVLQP
                     EGLRVGDIITYSYTLTRHIPELGAHAEGLLADGMPLLPVGHYLRRAVWDADLPVRWKT
                     TDDLGDLKATQKNDRKEVRFEARDYMPPKAADQAPLRDQMFSQMQFSNYASWSEVSAT
                     LAPLYDKAAKLAPDSPLRAELDKIRAAGADPAVQAAAALKLVENDVRYVYLGMNESGV
                     VPADADTTWTRRFGDCKGKTALLLALLHELGIKAEPALVSTSFGDGMDQRQPMAEYFD
                     HVIVRAEIGGKTYWLDGTRYGDRRLSDLRTPDYHWALPLRDAGAELVAMTPEPLDKPD
                     NEETLQLDASAGLDVPAKVHGERLIRGDDAVQLNASLAAMTVEDRDKALKSSWTSDYD
                     WLTASKVSAVFDPDKREERLIVDGEAKMNWDAPDINGRQYEADNYSLGWTGDLKRDPG
                     PHQNDPVALSYPFYSVWRESIVLPDHGKGFSLTGAPVDEPLYGKVFHRALSLKDGVFS
                     VESSMRSLAPEIPYAQAVRDQPAVTAIAKGSVYINAPFDYKASRTEPNSTKPVSGDAQ
                     AHADAAQDLAQQGRLGDALAEMDAAIKLKPDDADLLMKRGALNLVAHDIDKALADFDE
                     TVKRDPTQWEALDARGMALLVKRKPMDAIDDFSQSLELHSDNPMAREGRVKAYIANHQ
                     LDKALAEARMFARLYPELDEAQSTLASTLIDAKMYDEALDAYRKAVALAPDDADLLNE
                     FGDQLTQCNGATGAACDKQHAEALAIYDKVIAIEPTAHAYIARSQVRPPL"
BASE COUNT          465 a          780 c          769 g          358 t
ORIGIN      
        1 cacagctttc gcatcctgcg cggcgatcag gtcatcgacg tgctggcgca tcagggcttc
       61 accgtcgtgc ggcgcgagac caatctcgac cgcgccatgc tggacgggtt gctgaccggg
      121 gttttgcagc cggaaggctt gcgggtcggc gacatcatca cctacagcta cacgctgacg
      181 cgccatatcc ccgaactggg cgcgcacgcc gagggcctgc tggcggacgg catgccgctg
      241 ctgcccgtcg gccattatct gcgccgcgcg gtctgggacg cggacctgcc ggtgcgctgg
      301 aagaccacgg acgatctcgg cgacctcaag gcgacgcaga agaacgatcg caaggaggtg
      361 cgcttcgagg cccgcgacta catgccgccc aaggccgccg atcaggcgcc gctgcgcgac
      421 cagatgttca gccagatgca gttcagcaac tacgcctcat ggtccgaagt ctcggccacg
      481 ctggcgcccc tgtacgacaa ggcggcgaag ctggcgccgg actcgccgct gcgcgccgaa
      541 ctggacaaga tccgggccgc cggcgccgat cccgccgttc aggccgccgc ggccctgaaa
      601 ctggtcgaaa acgacgtgcg ctacgtctat ctcggcatga acgaaagcgg cgtcgtgccg
      661 gcggacgccg atacgacctg gacgcggcgc ttcggcgact gcaagggcaa gaccgccctg
      721 ctgctggccc tgctgcacga actggggatc aaggccgaac cggcgctggt cagcacgagc
      781 ttcggcgacg gcatggatca gcggcagccg atggctgagt atttcgacca cgtgatcgtg
      841 cgcgccgaga tcggcggcaa gacctactgg ctggacggca cgcgctacgg cgaccgccgc
      901 ctgtccgacc tgcgcacgcc ggactatcac tgggccctgc cgctgcgcga cgcgggcgcg
      961 gagctggtcg cgatgacgcc ggagccgctg gacaagcccg acaacgaaga gacgctgcaa
     1021 ctggacgcca gcgccggtct cgacgtcccg gccaaggtcc acggcgagcg cctgatccgt
     1081 ggcgacgatg cggtgcaatt gaacgcctcg ctcgccgcca tgaccgtcga agaccgcgac
     1141 aaggcgctga aatcgagctg gacaagcgat tatgactggc tgaccgccag caaggtttcc
     1201 gccgttttcg atcccgacaa gcgcgaggag cggctgatcg tcgatggcga agcgaagatg
     1261 aactgggatg ctcccgatat caatggacgg cagtatgagg cggataatta cagcctcggc
     1321 tggacagggg atctgaagcg cgatcccggt ccgcatcaga acgatccggt cgcgctcagc
     1381 tatcctttct attcggtatg gcgtgaaagc atcgtgctgc ccgatcacgg caagggcttc
     1441 agcctgaccg gcgcgccggt ggatgagccc ctctacggca aggtttttca tcgcgccctg
     1501 agcctgaagg acggcgtgtt cagcgtggaa agctcgatgc gcagcctggc gccggaaatc
     1561 ccctatgctc aggcggtcag ggaccagccc gccgtcaccg ctatcgccaa gggcagcgtc
     1621 tatatcaacg cgcccttcga ctataaggcc tccaggacgg aaccgaattc gacaaagccc
     1681 gtcagcggcg atgcgcaggc ccatgccgac gcggcgcagg acctggctca gcagggacgt
     1741 ctcggcgacg ccctggcgga aatggacgcg gcgatcaaac tcaagcccga cgatgccgac
     1801 ctgctcatga agcgcggcgc tctgaacctc gtagcgcatg atatagacaa ggccctggcc
     1861 gatttcgacg agaccgtaaa acgcgatccg acccaatggg aagctttgga tgcgcgcggc
     1921 atggccttgc tggttaagcg caaaccgatg gacgccatcg acgacttcag ccagtccctt
     1981 gagctgcatt cggacaaccc gatggcgcgc gagggccgcg tcaaggctta tatagccaac
     2041 catcagttgg acaaggcgct ggctgaggcg cgcatgttcg cgcgcctcta ccccgaactc
     2101 gacgaggcgc agagcacgct ggcgtcgacc ctcatcgacg cgaaaatgta cgacgaggcg
     2161 ctcgacgcct accgcaaagc cgtggcgctg gcgccggacg atgcggacct gctgaacgaa
     2221 ttcggcgatc aactgacgca atgtaacggc gcgacgggcg cggcctgcga caagcagcac
     2281 gcggaagctc tggcgatcta cgacaaggtg atcgccatag aaccgaccgc gcacgcctat
     2341 atcgcgcgct cgcaggtgcg tccgcctctc ga
//
LOCUS       DASVLL010000602         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_472643,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000602 DASVLL010000000
VERSION     DASVLL010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472643"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1677)
                     /gene="ligD"
                     /locus_tag="VF402_09890"
     CDS             complement(<1..>1677)
                     /gene="ligD"
                     /locus_tag="VF402_09890"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX7800650.1"
                     /translation="DVRDAILDGEIVAVDEHGATRFKLLQDALRDGHGENLQYYVFDL
                     LRLNGEDLRRLPLIERKTRLQALLDGYDFEDRVIYSEHFEAVKGFLAKVCGLGFEGLV
                     SKRRDAPYRGGRNKAWLKTKCHKRQEFVIGGFREPTHAARGIGALLLGYYDNGRLVYS
                     GKVGTGFDAETSRRLRARLDALTQDRPAYAEMPADMRRGAVWVKPELVCEVAFSEWTP
                     DGRLRHPSYQGLREDKPARDIGREQVAPEAQAMQQDNPKPSRRRAGNIEIGGVKISHP
                     DRVLWPQEGYTKQQMAEYYAAVADHILPHVAERPLSMVRCPDGIDGQCFFQRHIAHDM
                     SPHLYDTGVAVKGRDEDYMMIRDQAGLLTLAQWGVIELHPWGCRADRPDLPDRLILDL
                     DPDPDLSWAQVAAGAREARRRLEALGLRSWLKTTGGKGLHVVVPLQRRHGWPVVKAFA
                     RAVAEAMAADDPLYIAHARKADRKGRIFVDYLRNDLTATAVAPFSLRARPGAPASTPL
                     DWTDLTDELRPRDFNILTLPPLLSKRADPWSTFRDAPQTLTTEALRTMGL"
BASE COUNT          250 a          574 c          567 g          286 t
ORIGIN      
        1 ttgagtccca ttgtccgcaa ggcttccgtg gtcagggttt gcggcgcgtc gcggaaggtc
       61 gaccacgggt cggcgcgttt cgacagaagg ggcggaagcg tcaggatgtt gaaatcgcgc
      121 ggcctcaact cgtcggtcag gtccgtccag tcgagcggcg tcgacgccgg ggcgccgggg
      181 cgggcgcgta aagaaaaggg cgcgaccgcc gtggcggtca ggtcgttgcg caggtagtcg
      241 acgaagatgc gccccttgcg gtcggccttg cgcgcgtggg cgatgtagag cgggtcgtcc
      301 gccgccatcg cctcggcgac ggcgcgcgcg aacgccttga cgacgggcca gccgtgacgg
      361 cgttgcagcg ggaccacgac atgcagcccc ttgccgccgg tggtcttgag ccagcttcgc
      421 aggcccagcg cctccagccg ccgccgcgcc tcgcgcgccc ccgccgccac ctgcgcccac
      481 gaaaggtccg ggtcgggatc gagatcgagg atcagccggt cgggcaggtc cggccggtcg
      541 gcgcggcagc cccacgggtg cagttcgatc acgccccatt gcgccagggt cagcaggccc
      601 gcctgatcgc ggatcatcat gtaatcctcg tcgcggccct tgaccgccac gccggtgtcg
      661 taaaggtgcg gcgacatgtc gtgcgcgata tgacgctgaa agaagcattg cccgtctatg
      721 ccgtccgggc agcgcaccat cgacagcggg cgctccgcga cgtgcggcag gatatggtcg
      781 gcgacggcgg cgtaatattc ggccatctgc tgcttcgtgt agccctcctg cggccacagc
      841 acgcggtcgg gatggctgat cttcacgccg ccgatctcga tgttcccggc gcggcggcgc
      901 gagggtttcg gattgtcctg ctgcatggcc tgagcctccg gcgcgacctg ttcgcggcct
      961 atgtcgcgcg cgggcttgtc ttcgcgcaag ccctgatagg acggatgacg cagccgcccg
     1021 tccggcgtcc attcgctgaa cgccacctcg cacaccagtt cgggcttcac ccagaccgcg
     1081 ccgcgtctca tatcggcggg catttccgca taggcgggcc tgtcctgcgt cagcgcgtcc
     1141 agtctggcgc gcagccttcg cgacgtctcc gcgtcgaagc ccgtgccgac cttgccggaa
     1201 tagacgaggc gtccgttgtc gtaatagccg agcagcagcg cgccgatccc gcgcgcggcg
     1261 tgggtcggct cgcggaagcc gccgatgacg aattcctgcc gtttatggca cttggtcttg
     1321 agccacgcct tgttgcgccc gccgcgatag ggcgcgtccc ggcgcttgga caccagcccc
     1381 tcgaagccca gcccgcacac cttggcgaga aaccccttaa cggcctcgaa atgctcggaa
     1441 tagatgacgc gatcctcgaa atcgtagccg tctagcagcg cctgcaaccg cgtcttgcgt
     1501 tcgatcagcg gcaggcggcg caggtcttca ccgttcagcc gcagcaggtc gaaaacgtaa
     1561 tattgcaggt tttcgccatg cccgtcgcgc agggcgtcct gcaacagctt gaagcgcgtc
     1621 gcgccgtgtt cgtcgaccgc gacgatctcg ccgtcgagga tcgcgtcgcg cacgtcg
//
LOCUS       DASVLL010000603         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_417033,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000603 DASVLL010000000
VERSION     DASVLL010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417033"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..672
                     /locus_tag="VF402_09895"
     CDS             181..672
                     /locus_tag="VF402_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7800651.1"
                     /translation="MGRSADYSRQRDASAACLDIIGEPWTLLIIRDAFRGLRRFEQWQ
                     DSLGLARNVLAARLKHLVAHGVFETRVYCERPQRHEYLLTEKGRALRGILLHMVDWGQ
                     TYVYQDEKPLIEGFHTCGHRIKPVLYCGHCQQPMLPGEVERHRNPDAKSIAELARLAM
                     AAE"
     gene            complement(720..1301)
                     /locus_tag="VF402_09900"
     CDS             complement(720..1301)
                     /locus_tag="VF402_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7800652.1"
                     /translation="MIARIQEPIPATRGTAPESTIETTRLSLTGFRPADAAALQAVFA
                     DPATARMTHAIPHPFTPDDALTKLARMMQAPDAHYAIRTGDEALIGVVSLTRGACGQA
                     ADLHTFGPNLSVFIAPAHQRQGYATEAIDGLLRRVKKRKLHRVIHAAHFADNLASARL
                     LVHADFLYTGRRTMETSRAREGQHQALHMIRLL"
     gene            complement(1545..1814)
                     /gene="rpmA"
                     /locus_tag="VF402_09905"
     CDS             complement(1545..1814)
                     /gene="rpmA"
                     /locus_tag="VF402_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7800653.1"
                     /translation="MAHKKSGGSSRNGRDSEAKRLGVKRFGGENVLAGNIIVRQRGTK
                     FWPGTNVGLGRDHTLFATANGQVKFTTKRDNRCYVSIEPMAQAAE"
     gene            complement(1840..2364)
                     /gene="rplU"
                     /locus_tag="VF402_09910"
     CDS             complement(1840..2364)
                     /gene="rplU"
                     /locus_tag="VF402_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7800654.1"
                     /translation="MYAVIKTGGKQYRVAAGDLIKVEKLEGEAGAAVTFNDVLMIGDE
                     AGAVVGAPLVDGAAVEATLIETRKGEKVRIFKKIRRQGYRRTNGHRQLESVLRVTGIS
                     GAGKSAKWDGTVSLTPLAELNARARNLAPRGLAAAIETPAVAAPVAEEAPAPAKKKAA
                     PKKKAAADSEAGEA"
     gene            2961..>3052
                     /locus_tag="VF402_09915"
     CDS             2961..>3052
                     /locus_tag="VF402_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7800655.1"
                     /translation="MPDAATTSQKTPVTVLTGYLGAGKTTLLNR"
BASE COUNT          549 a          942 c          965 g          596 t
ORIGIN      
        1 tcgtgcgcag cgacgaggac atgatggcgc atgccctcgc ggcgctgtac gccgcgaatc
       61 cctgaaaggc cgagccgata aaatccgtct cgcgtctttt ttggcgacgg aacggcgggc
      121 gtcttgcgtg tcaatcgcag cctcggcgcc acgccgccga cgccgtcagg atacatgctc
      181 atgggtcgtt ccgccgatta ttcccgccaa cgcgacgcgt ccgccgcctg cctcgacatc
      241 atcggcgagc cgtggaccct gctgatcatc cgcgacgcgt ttcgcggcct gaggcgcttc
      301 gagcagtggc aggactcgct gggtctggcg cgcaacgtgc tggcggcgcg cctcaagcat
      361 ctggtcgcgc acggcgtgtt cgagacgcgc gtctattgcg agcgcccgca acggcatgaa
      421 tatctgctga ccgaaaaggg acgcgcgctg cgcggcatcc tgctgcacat ggtcgactgg
      481 ggccagacct atgtttatca ggacgaaaag ccgctgatcg agggctttca cacctgcggc
      541 caccgtatca agccggtcct ctattgcgga cattgccagc agcccatgct gcccggcgag
      601 gtcgagcgcc accgcaaccc ggacgccaag tcgatcgccg agctggcccg gctggccatg
      661 gccgcagaat agaaaagggc ggcggaatag aaaagggagc cctgcggctc ccctttcctt
      721 tataaaagcc ggatcatgtg cagcgcctga tgctggcctt cgcgcgcgcg cgacgtctcc
      781 atcgtgcgcc gtccggtata gaggaaatcg gcgtggacca gcagccgcgc cgaggccaga
      841 ttgtcggcga agtgcgcggc gtggatgacc ctgtgaagct tgcgcttctt gacccggcgc
      901 agcagcccgt cgatggcctc ggtggcgtag ccctggcgct gatgcgcggg cgcgatgaag
      961 acgctgaggt tcggcccgaa cgtgtgcaga tcggccgcct ggccgcacgc cccgcgcgtc
     1021 aggctgacga cgccgatcag cgcctcgtcg ccggtgcgga tcgcatagtg cgcgtccggc
     1081 gcctgcatca tgcgcgccag cttggtcagc gcgtcgtccg gcgtgaacgg gtgcggaatc
     1141 gcatgggtca tgcgcgccgt ggccggatcg gcgaacacgg cctgaagcgc ggcggcgtcg
     1201 gcgggccgga agccggtcag gctcaggcgc gtggtctcta tcgtgctttc cggggcggtc
     1261 ccgcgggtgg cggggatcgg ttcttgaatg cgcgcgatca tagctcgtct cctctcgccc
     1321 gtgacgcagc gttttcctta tcgccgtcgt ctgggaccta tttagggtcg aactgtgtca
     1381 gttccacccc aggggatttt tgagcgggat cacatttttc agaggatgcg tgcgcatcgc
     1441 gatccgaaat gcaaaacggg agcccggtgg aatccggact cccgctcaaa ctcaggtccg
     1501 gatcggctta tttcgtaagc acgatccgaa agtaaggagc atgcttattc ggcggcctgc
     1561 gccatcggtt cgatcgaaac gtaacagcgg ttgtcgcgct tcgtggtgaa cttcacctga
     1621 ccgttggcgg tggcgaaaag cgtgtggtcg cggccgaggc cgacattcgt acccggccag
     1681 aacttggtgc cgcgctgacg cacgatgatg ttgccggcga ggacgttctc accgccgaag
     1741 cgcttcacgc caaggcgttt ggcttccgag tcgcgaccgt tgcgggacga accgccggat
     1801 tttttgtgag ccatgggagg atctccttaa atcttgcgct tacgcttcgc cggcttcgga
     1861 gtcggcggcg gccttcttct tcggcgcggc cttcttcttg gcgggagcgg gggcttcctc
     1921 agcgacgggc gctgcgacag ccggggtttc gatggcggcg gccagaccac gcggagcgag
     1981 gttgcgcgcg cgggcgttca gctcggccag cggggtcagc gagaccgtac cgtcccactt
     2041 ggccgacttg ccggcgccgg aaatgccggt gacgcgcagg accgattcca gttggcggtg
     2101 gccgttggtg cggcgatagc cttgacggcg gatcttcttg aagatgcgga ccttctcgcc
     2161 cttgcgggtt tcgatcaggg tcgcctcgac cgccgcgccg tcgaccagcg gagcgccgac
     2221 cacggcgccg gcttcgtcgc cgatcatgag gacgtcgttg aacgtcacgg cggcgccggc
     2281 ttcgccttcc agcttttcga ccttgatcag atcgccagcc gcaacgcggt attgcttacc
     2341 gcccgttttg atgaccgcgt acatggtcag aaccctatgc agtggcgcaa ggcatgtgca
     2401 gggtctgcaa ggcttgcgcg ggaaagaaaa ggaaaacgcc ccagaagacg caaggccttc
     2461 gggcgagagg cggcgactta tacagaggaa tgcgggtgag tcaaccggca tcagccccta
     2521 aaatccaaaa ttggaaagtc ttctcttagc tttcgggtta gctcctcata actcaaggtc
     2581 gcaagttgtc cctgccccat aattgctgga gataccgtca ttcgtgcatc gggaagactt
     2641 ccaactcgct cgtccgtttt gtccagtccg agcttaattc tgcgtagctc gtgttcgttt
     2701 tgacgatcag cgaaatgacc taatattatt gctgccgccc ctacggcgca agctgcccag
     2761 gcttggattg gccctaaaat agcggctagc agtcctaaga ctcccgcaga cactcccaaa
     2821 tgacggcgta acatccttaa cattacaatt acctcttata tcggtcgagt atgacagatg
     2881 aagaaactaa gcggacttga tttcgcgccg caatggcata cttaaaagcc gattataaag
     2941 taaccttcaa aaggcccgcc atgcccgacg ccgctacaac ctcgcaaaag actcccgtca
     3001 ccgtgctgac cggctatctg ggcgcgggca agacgacgct tctgaaccgc at
//
LOCUS       DASVLL010000604         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_40258, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000604 DASVLL010000000
VERSION     DASVLL010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40258"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF402_09920"
     CDS             complement(<1..236)
                     /locus_tag="VF402_09920"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX7800656.1"
                     /translation="MSESVSAPADRSALVLFSGGQDSSVCLAWALARYERVETVGFDY
                     GQRHSIEMETRQSVLAKFRKVYPPAKARLGEDHV"
     gene            393..836
                     /locus_tag="VF402_09925"
     CDS             393..836
                     /locus_tag="VF402_09925"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7800657.1"
                     /translation="MNSTPFHDMFADIIANTPYMKAIGAEYAGRGQDGVTLRLPYRAD
                     LVGDPETRVIASGVVTALLDNTCGMAVWDRIGEFKPVATLDLRIDYMRPALPDNALLA
                     TASCYKLTRSVGFVRAFAYEVSPDDPVAAAQAAFVISSPTLRTKV"
     gene            842..1264
                     /locus_tag="VF402_09930"
     CDS             842..1264
                     /locus_tag="VF402_09930"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7800658.1"
                     /translation="MQTLSQIVHHVEPETVLRAMPFAAFLGVRLSLAGDELTAQLPFQ
                     DKLIGNPMIPALHGGVIGAFMEVTAMAQLALSENFDHIPKPIDVTVQYLRSGRAIDAF
                     ARAVLNRVGRTVANVEVCAWQERRDQPIAALQAHFLIE"
BASE COUNT          296 a          509 c          453 g          262 t
ORIGIN      
        1 cagacatggt cctcgccgag gcgcgccttc gcgggcgggt aaaccttgcg gaacttcgcc
       61 agcacgctct ggcgcgtctc catctcgatg ctgtggcgct ggccgtagtc gaagccgacc
      121 gtctcgacgc gctcataacg cgccagcgcc caggccaggc acaccgacga atcctgtccg
      181 ccggaaaaca gaaccaaggc ggagcggtcg gcgggagcgg agacggattc ggacatggcg
      241 gaaagctttc aggcgcaact ggaaacggcc tatgtaaaaa gcccctttgc cttgaattgc
      301 aagttttttt gctgcgccgc atgatgacgt gaacgtaaag ttaggccaag atgcccgcaa
      361 agccgcaagc gcggcgaagt caggaggcgc gcgtgaactc tacgcctttt cacgacatgt
      421 tcgcggacat catcgccaac acgccctaca tgaaggccat cggcgccgaa tacgccggac
      481 gcggccagga cggcgtcacc ctgcggctgc cgtaccgcgc cgatctggtc ggcgacccgg
      541 aaacgcgcgt catcgcctcc ggcgtggtca ccgccctgct cgacaacacc tgcggcatgg
      601 cggtgtggga ccgcatcggc gaattcaagc cggtcgccac gctggacctg cgcatcgact
      661 atatgcgccc ggccctgccc gacaacgcgc tgctggccac ggccagttgc tacaagctga
      721 cccgcagcgt cggcttcgtg cgcgccttcg cctacgaggt cagccccgac gatccggtgg
      781 cggcggcgca ggcggccttc gtcatctcct cccccacgct caggaccaag gtttgaagcg
      841 catgcagacc ctgtcgcaga tcgtacacca cgtcgaaccg gaaaccgtcc tgcgcgccat
      901 gccgttcgcg gcgtttctgg gcgtgcggct gtccctggcc ggggacgagc tgacggcgca
      961 actgccgttt caggacaagc tgatcggcaa tccgatgatc ccggcgctgc acggcggcgt
     1021 catcggcgcc ttcatggagg tcaccgccat ggcgcaactg gcgctgagcg aaaatttcga
     1081 ccacatccca aagcctatcg acgtcacggt gcaatatctg cgctccggcc gggccatcga
     1141 cgcgttcgcg cgcgccgtgc tcaatcgcgt cggccgcacg gtcgccaatg tcgaggtctg
     1201 cgcctggcag gagcggcgcg atcagcccat cgccgccttg caggcgcatt tcctgatcga
     1261 atgacaattt atacgcgtaa aaacattgta tgctcgtaat tatagaatta ccctgagcag
     1321 gagggccgtg attcgctcgc cctcatttct tcaactcagg gagctaccgt atgtccaaac
     1381 aaagaaagat caatgtcgcc aaggcattgg ctgtccaact tcacgccacg gaagaagcca
     1441 tcgaaacggc gctggccgaa gcggcccacc tgatcgaggc ctacgtcaat tcgcgccgcg
     1501 cgattcgcct gtccacggtc
//
LOCUS       DASVLL010000605         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_417162,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000605 DASVLL010000000
VERSION     DASVLL010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417162"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>1892
                     /locus_tag="VF402_09935"
     CDS             19..>1892
                     /locus_tag="VF402_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800659.1"
                     /translation="MKDYLLLILLLVPSGVLAQPPDDAGADAPSIIVTGQGLGLPPGT
                     PAYGSVAIDRQRLLDNASGRLENVLANVAGSQQFRRSDSRASNPSNQGATLRALGGNA
                     SSRTLVLLDGVPVADPFFGYIPFSALVPDRLSVVRVTRGGGSGAFGAGAVAGTIELAS
                     ATRDQLPDFAASAFYGSHDAAEMSASVTPDLGGGYVSLSGRWDRGNGFQTTPKSQRVA
                     ATVPAAYDSWSTNLRAVAPLSVTSELQFRATIFQDDRTLRFKGADSANEGQDASVRFI
                     SRGDWQVDALAYIQARNFSNIVISASTFRKSLDQRNTPSMGLGGKIEIRPPVGSDHVL
                     RLGADTRFALGDMYEDAYDANIASNPVTFRRHAGGQQVTTGLFAEDDWTLEGLVLTGG
                     VRADHWTISDGFYHQANASTGAVTATDYANRSDWQFSGRAGAILRLSGALALRGAGYT
                     GFRLPTLNELYRPFVVFPITTQANAALKPERLKGVEAGFDLTPLPGVTLGATAFYNRL
                     DDAIANVTIGTNTRQRQNVNRIVAKGIELTASARLGDFGLSASYAYSHSEVDAPGKAF
                     DGFAPAQTPRNSASATLAWTPAQGPTLSASVHYVSSQYEDDLQIDTLPRALTVDALAR
                     "
BASE COUNT          297 a          635 c          635 g          325 t
ORIGIN      
        1 agagggcgtg gacgcatcat gaaagactat ctgctgctga tacttctgct ggtgccatcc
       61 ggagtgctgg cgcagccccc cgatgatgca ggcgccgatg caccttcgat catcgtaacg
      121 ggacaggggc tgggcctgcc acccggtacg cccgcctatg gatcggtggc gatcgaccgg
      181 caacggctgc tcgacaatgc gtcgggccgg ctggagaatg tgctggccaa cgtcgccggc
      241 tcccagcagt ttcgtcggtc ggacagccgg gcgtcgaacc cgtccaacca gggcgcgacc
      301 ctgcgggcgc tgggtggcaa tgcatccagc cggacgctgg tgttgctgga cggcgtgccc
      361 gtggccgatc ccttctttgg ctatattcct ttctccgcgc tggttccgga ccgtctgtcc
      421 gttgtgcggg tgacgcgcgg cggcgggtcg ggtgcgttcg gcgcgggcgc tgtcgccggc
      481 acgatcgagc tggccagcgc cacgcgcgac cagttgccgg atttcgctgc cagcgccttc
      541 tatggcagcc atgacgccgc cgagatgtcg gccagcgtca cgccggacct aggcggcggc
      601 tatgtttcgc tgtcggggcg ctgggaccgg gggaacggct tccagaccac gccaaagtcg
      661 cagcgcgtcg cggcgaccgt gcccgccgcc tatgatagct ggtccaccaa tttacgcgcg
      721 gtcgcgccgc tctccgtgac gtccgaattg cagttccgcg ccacgatctt tcaggacgac
      781 aggacgctgc ggttcaaggg cgcggactcc gcgaacgaag ggcaggacgc cagtgttcgc
      841 ttcatctcgc gcggcgattg gcaggttgat gcgctcgcct atatccaggc gcgcaacttt
      901 tccaatatcg tgatctccgc ctccaccttc cgcaagtcgc tcgaccagcg caacacgccg
      961 tccatggggc ttggcggcaa gatcgagatc cgcccgccgg tcgggtcgga tcatgtactg
     1021 cggctgggcg cggatacgcg cttcgcgctg ggcgacatgt atgaggacgc ctatgacgcc
     1081 aatatcgcca gcaatcccgt caccttccgc cgccatgccg gtgggcagca ggtgacgacc
     1141 ggcctgttcg ccgaggacga ctggacgcta gaaggcctgg tgttgaccgg cggcgtgcgg
     1201 gcggatcact ggacgatcag cgacggcttc tatcatcagg ccaacgccag caccggtgca
     1261 gtgacagcga ccgattacgc caaccggtcc gactggcagt tttcggggcg ggcgggcgcg
     1321 atcctgcgcc tgagcggcgc gctcgcgctg cgcggggcgg gctataccgg ctttcgcctg
     1381 ccgacgctga acgagctgta ccggccattc gtggtcttcc cgatcacgac gcaggcgaac
     1441 gcggcgctga agcccgagcg cctgaagggc gtggaggcgg gcttcgacct gaccccgctg
     1501 cccggcgtga ccctgggcgc gaccgccttc tacaaccggc tggacgatgc gatcgccaat
     1561 gtcaccatcg gcaccaacac ccgccagcgc cagaatgtta accgcatcgt cgccaagggg
     1621 atcgaactta cggccagcgc gcgtctgggc gatttcggcc tgtcggcctc ctatgcctac
     1681 agccacagcg aggtcgatgc gccaggcaag gcgttcgacg gcttcgcgcc cgcgcagacg
     1741 ccgcgcaaca gcgccagcgc gacgctggcc tggacgccgg cgcaggggcc gacgctctcc
     1801 gcgtcggtcc attatgtgtc gagccaatat gaggatgatc ttcagatcga cacactgccc
     1861 agggcgctga ccgtggatgc gctcgcgcgc tt
//
LOCUS       DASVLL010000606         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_417519,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000606 DASVLL010000000
VERSION     DASVLL010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417519"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF402_09940"
     CDS             <1..380
                     /locus_tag="VF402_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800660.1"
                     /translation="PMWDGVMSIYSGNATLIKNVVFSDINVHRIEEGRLFNIVAGQTK
                     LTIVSAQANKRPGRGIDGVTIRNISFDGDGMPGRSIISGLAPGTEVRNVRIQNLRIGG
                     RRVVSAAAAGIDIGPEVKNFTIH"
     gene            complement(428..>2743)
                     /locus_tag="VF402_09945"
     CDS             complement(428..>2743)
                     /locus_tag="VF402_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7800661.1"
                     /translation="GPPGRRYSLEFEGAFQDARVYVNGVELAHHRGGYTGFPVDITPA
                     VRPGRNVVAVKVNNAWSATLAPRAGEHVFSGGLYRDVWLVSTDAVHVPWTGTRVTTPD
                     LSAASGKVRVDTEVSNSGAVPVEVTVDTRIIDDRGAVVATLPRGHVRLAPGEMQIVSQ
                     LSAPIAKPRLWSPDTPVLYRAVTRLSVGGRDRDRFETEFGFRWFEWTADHGFFLNGKH
                     LYFRGANVHQDQAGWGDAVTNGAIERDVRMLKDAGFDFIRGSHYPHDPHFAETTDKVG
                     MLFLSEAPFWGTAGFKNPWGAPAYPTDPADFAAFDANVKQQLAEMIRINRNHPSIIAW
                     GMDNEVFFTTRETMPEVRRLLKEMVALSHKLDPTRPASIDGAQRGDIDKLGDIAGYNG
                     DGASLFPDPGIPNFVAEYGETMTERPGDYAPGWGDLPNTPGADKTKPGSWRLPWRSGE
                     AIWAGFDHGSIAGKRFGGMGMIDYFRLPKRQYYWYRNAYAHVPPPVWPVEGAPAALRV
                     TTSSPVIRRADGTDDVQVIVSVVDAEGRRLSNSPPVRLAIESGPGELPTGRAIDFAPD
                     SDILIRDGEAAIAMRSWQAGITRLRATSPGLKDGVATVRTLNGPPFRPGVTPLAPDRP
                     YTPYVAPVRARPGDGVFGLNNPTFASSSAPDHSSRLVNDGDAATYWAPAPGDAEMTLT
                     LDMERVVEVHALTLTFPQAGPYGFLAEIQDAQGNWRKLAEQVEGQDTSAVRTVETEAR
                     EGRKVRIHLRAPAGYPAGLAELRVGGALHTD"
BASE COUNT          428 a          910 c          950 g          455 t
ORIGIN      
        1 atccgatgtg ggacggcgtt atgtcgatct attcgggcaa tgcgactctg atcaaaaacg
       61 tcgtctttag cgatatcaac gtgcatagaa ttgaagaggg aaggctgttc aatattgtcg
      121 caggccagac gaagttgacc atagtcagcg ctcaggcgaa caagaggccc ggccgcggca
      181 tcgatggcgt cacaatccgc aatatcagct ttgatggaga cggcatgcct ggccggtcga
      241 tcatcagcgg cctggcgccg gggaccgaag tcaggaacgt gcgaattcag aatttgcgaa
      301 tcggcggccg ccgggtcgtc tccgccgccg cagccggtat cgatattggc ccggaagtga
      361 agaatttcac gatccattag tcggcgtgcg cccgcggcga agccgggtcg ccccgccgcg
      421 gcgccgatca atccgtatgc aacgcgccgc cgacccggag ttcggcgagg cccgcggggt
      481 agcctgcggg cgcgcgcaga tggatgcgga ccttgcggcc ttcgcgcgcc tcggtttcga
      541 cggtgcggac cgcgctcgtg tcctggccct cgacctgttc ggccagcttt cgccagttgc
      601 cctgcgcgtc ctgaatctcg gcgaggaagc cgtagggacc ggcctgcggg aaggtcaggg
      661 tcagggcgtg gacctcgacc acgcgctcca tgtcgagggt cagggtcatc tccgcatcgc
      721 ccggcgcggg cgcccagtag gtggcggcgt cgccgtcatt gaccagccgc gagctgtggt
      781 ccggcgcgct ggagctggcg aaggtcgggt tgttcaggcc gaacacgccg tcgcccggcc
      841 gtgcgcgaac gggcgcgacg tagggcgtat agggccggtc gggcgccagc ggcgtcacgc
      901 cgggcctgaa aggcggtccg ttcagcgtgc gcaccgtggc cacgccgtcc ttcaggccgg
      961 gcgacgtggc gcgcagccgc gttatcccgg cctgccagct gcgcatggcg atggcggctt
     1021 cgccgtcgcg gatcaggata tcggagtcgg gcgcgaaatc gatggcgcgc ccggtcggca
     1081 gttcgcccgg acccgattcg atggccagcc ggaccggcgg gctgttgctg aggcgccgtc
     1141 cctcagcgtc gaccacgctg acgatgacct gcacatcgtc cgtgccgtcg gcccggcgga
     1201 tcaccggcga gctggtcgtg acgcgcagcg cggcgggcgc gccctcgacc ggccacacgg
     1261 gcggcggcac atgcgcatag gcgttgcggt accagtaata ttgccgcttg ggcaggcgga
     1321 aatagtcgat catccccatg ccgccgaacc gctttccggc gatggaccca tggtcgaagc
     1381 ccgcccagat cgcctcgccc gaacgccacg gcagccgcca cgagcccggc ttcgtcttgt
     1441 ccgcgcccgg cgtgttgggc aggtcgcccc agccgggcgc atagtctccg ggccgctcgg
     1501 tcatcgtctc cccgtattcg gcgacgaagt tcgggatgcc gggatcgggg aacaggctgg
     1561 cgccgtcgcc gttgtaaccg gcgatgtcgc ccagcttgtc gatatcgccg cgttgcgcgc
     1621 cgtctatcga cgccgggcgc gtcgggtcga gcttatgcga cagggcgacc atctccttca
     1681 gcaggcggcg gacctccggc atagtctccc tggtcgtgaa gaacacttcg ttgtccatgc
     1741 cccaggcgat gatcgacgga tgattgcggt tgatgcggat catttcggcc agttgctgct
     1801 tcacattggc gtcgaaggcc gcgaagtcgg cggggtcggt cggataggcc ggggcgcccc
     1861 acgggttctt gaagcccgcc gtgccccaga acggcgcctc cgacaggaac agcatgccca
     1921 ccttgtcggt ggtctcggcg aaatgcggat cgtgcggata gtgcgagccg cggatgaagt
     1981 cgaagcccgc gtctttcagc atccgaacgt cgcgttcgat ggcgccgttg gtgacggcgt
     2041 cgccccagcc cgcctgatcc tgatggacgt tcgcgccgcg gaagtagaga tgcttgccgt
     2101 tcaggaagaa gccgtggtcc gccgtccatt cgaaccagcg gaagccgaat tcggtctcga
     2161 agcggtcgcg gtcccgcccg ccgacgctca gccgcgtcac cgcgcggtaa agaaccggcg
     2221 tgtccggcga ccacagacgg ggttttgcga tcggcgccga tagctggctc acgatctgca
     2281 tttcgcccgg cgccagtcgc acatggcccc tgggcagggt cgcgacgacc gcgcccctgt
     2341 cgtcgatgat gcgcgtgtcc accgtgacct cgaccgggac ggcgccgctg ttgctgacct
     2401 cggtgtcgac cctgaccttg ccggaggccg ccgaaaggtc gggcgtcgtc acgcgcgtgc
     2461 ccgtccaggg gacatgcacc gcatcggtcg acaccagcca gacgtcgcgg tacaggccgc
     2521 cgctgaagac atgttcgccc gcgcgcggcg ccagggtcgc gctccaggcg ttattgacct
     2581 tgaccgcgac gacattgcgg cccggccgca ccgccggcgt gatgtcgacg ggaaaaccgg
     2641 tgtagccgcc ccggtgatgc gccagctcca cgccgttgac atagacgcgc gcgtcctgaa
     2701 aagcgccctc gaattcaagc gaatagcggc ggccgggcgg ccc
//
LOCUS       DASVLL010000607         6899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_527319,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000607 DASVLL010000000
VERSION     DASVLL010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527319"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2629
                     /locus_tag="VF402_09950"
     CDS             <1..2629
                     /locus_tag="VF402_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800662.1"
                     /translation="SAQIVEAVSAEDIGKLPDQSIAESLARLPGIAAQRTNGRAQTLA
                     IRGLGPDFTVTTLNGREQVSTNDNRSVEFDQYPSELISQVLVYKTPNAGMTDQGIAGT
                     ADLQTVRPLSYGKRALAVSVRGEQNSEDARIGGMKNTGDRYSVTYIDQFFDKKVGIAL
                     GYAHTESPYQTLKKESWGYPTCSACAAADQNLLVTGGEKDAVDSSVLNRDGYMGVLEF
                     KPNDRLHMTFDAYHSDFNELQRIARLEYPLAWGANHLEPGYTTDNEFVTSGTFDDVKT
                     VVENYVNQRKDKLDSYGWNTTYDLNDKWSLMADLSESKADRVDVQVESTAGTGPNGSG
                     ATDTVSFTQQGFGLTSLKNAIDYSDFDTIFLTDPGGWGGLAPNRAGYAREPHVTDKID
                     SIKLAATRHFGGGFLSDVSFGYDHTEHRKHKEGVEGYLGLDGVSEASVPDQYRTGTTD
                     TGFLGSAGGMISYDALALYESGFFKFYEEVAAAAVQKTWTVNEKLDTFYVKADIDTTL
                     WGVPLTGNMGLQSVHADQRATSFYTDGTADGVTPQSGGASYTDILPTLNLNFQLADDT
                     TLRFGAGTTIARPRMDDMAAGTSYSTSVNTNPSVIGTQKYYWSGGGGNPALRPWKANN
                     FDLSVEHYFGRKGYISGALFYKDLTTFIYSQTLLKDYTGVDPSGACWSDAAHSVKVVD
                     ADKTSPYYGQWVCAMADANRIGTTTAQANGHGGYIKGVELTVSMPGELIASWLDGFGV
                     IWSASYNASSINPSGNNAYDVPGLSKEVMNTTLYYEKHGFSARISNRYRGDFLGEVPD
                     YTNSLSNTWVHSENIVDAQIGYSFDSGPLKNLSINISGSNLTNEPFYTYQGKGLPDHI
                     LRYEKYGSTYLFGVSYKFF"
     gene            2736..3536
                     /locus_tag="VF402_09955"
     CDS             2736..3536
                     /locus_tag="VF402_09955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7800663.1"
                     /translation="MPDQDASAKTSPFGFRDVDAREKVAMVHGVFRNVASKYDLMNDV
                     MSGGVHHLWKDAACARLNPQPGEVIVDCAGGTGDIARRLAKLARRARARREARGFEAA
                     DADIRIIDYNEAMIMAGREKSERKALYEPEISWSVGDAMNLALPDDCIDAYIISFGIR
                     NVADVQAALGEAYRVLKPGGRFFCLEFSHPESPVVEKVYEAYSFKLIPFMGKIVANDR
                     DSYQYLVESIKRFPDQETFRGMMQKAGFDRVTYTNYSGGVCALHEGWK"
     gene            3546..5057
                     /gene="ubiB"
                     /locus_tag="VF402_09960"
     CDS             3546..5057
                     /gene="ubiB"
                     /locus_tag="VF402_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEX7800664.1"
                     /translation="MAHLGSWLRLTRAGFTLLRYDALIPKELSPLLPPIPRFFAGLLR
                     GLFARRSRLRVGERYALAFERLGPAFIKLGQVLSTRGDIFGERFIADLTHLKDSLPPF
                     AKTEAEAAILDAFGKPVAAIFADFGEVIGSASIAQAHRAALLDGREVAVKILRPGIEL
                     TVARDVDMLLLGARLVAALVPASRRLEPEAFVATVAKSLQLELDLRLEASACDEIGKI
                     INDEPWMKAPAVVWSHVSRRVIVTEWADGVPLSRPEALDLPGLDRKALADNLIRAFLS
                     QSLDHGVFHADLHEGNLFVAAPARITAIDFGIIGRLRPKERRYLAEMLWGFLRRDYKR
                     VAEVHFEAGYVPAHQNVEAFAQALRAVGEPVLGRQASDVSMGRLLTQLFEITAQFDMH
                     LRPELVLLQKTMVSVEGVARRIYPEHNLWEAARPVVKAWISRELSPVAEAKKLLETLI
                     RRLNDDPSERIRDALTARDSLRADLRRSQGLALVAVIVSALSLGVMIWLALSK"
     gene            5173..6048
                     /locus_tag="VF402_09965"
     CDS             5173..6048
                     /locus_tag="VF402_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX7800665.1"
                     /translation="MAIGVFDSGIGGLSIHRALVEVMPRADFIYLADQKNTPYGDKPG
                     EAIVELTRAGCQTLFDAGASLVVLACNTASAVALRRLQSTWLVDYRKTCGRPVNVLGI
                     IVPTIEAATGLPWEYAADRRGPKIEKLDVIGVFATAATARSRVYEIEIDKRRDDLAVF
                     TVACSGLADMIEGAAPRARIRELIASYAQALRTRIGRWPDRAILGCTHYEIVADLFRE
                     VLPEGTELIGQPAPVAAALADYVRRHPEYATGRGGGRRFLTTGDPGAQPPLAEAFWGG
                     PLAFEPVQTKAVQGF"
     gene            complement(6036..>6899)
                     /locus_tag="VF402_09970"
     CDS             complement(6036..>6899)
                     /locus_tag="VF402_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX7800666.1"
                     /translation="SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSAGPITDSAWV
                     PAGYTLVWNDEFDGSGLPDSGKWSDDTNANTFDPGNGELEYYTAGDLDNERQDGDYLY
                     LTARKEAATGQPNYAGQAYTSARIVTRGKSSWLYGFVDVRAKLPCGAGAWPAIWMLGD
                     GGSWPADGEIDIMEQLGKDPNTIYGTIHDTSNQAHNGDGAHTTISDACGNFHNYELTW
                     TADEIDIGVDGVIYQTYANAHTGKDQWPFDEKQYLLLNLALGGSWAGPVDDGALPESM
                     VVDYVRVYQKP"
BASE COUNT         1202 a         2291 c         2229 g         1177 t
ORIGIN      
        1 gagcgcgcag atcgtcgaag ccgtgtcggc cgaggacatc ggcaagctgc cggaccagtc
       61 gatcgccgaa tcgctggcgc gcctgcccgg catcgcggcg cagcgcacca acggccgcgc
      121 ccagaccctg gccatccgcg gcctcgggcc ggacttcacc gtcaccaccc tgaacggacg
      181 cgagcaggtg tcgaccaacg acaaccgctc ggtcgagttc gaccagtatc cgtccgagct
      241 gatctcgcag gttctggtct ataagacgcc gaacgcgggc atgaccgatc agggcatcgc
      301 cggcaccgcc gatttgcaga ccgtgcgtcc gctgtcctac ggcaagcgcg ccctggccgt
      361 gtccgtgcgc ggcgagcaga acagcgaaga cgcccgcatc ggcggcatga agaacaccgg
      421 cgaccgctat tcggtcacct atatcgacca gttcttcgac aagaaggtcg gcatcgcgct
      481 cggctacgcg cacaccgagt cgccgtatca gacgctgaag aaggaatcgt ggggctatcc
      541 gacctgttcg gcctgcgccg cggcggacca gaacctgctg gtcaccggcg gcgaaaagga
      601 cgcggtcgac tcctcggtgc tgaaccgcga cggctatatg ggcgtgctgg agttcaaacc
      661 gaacgaccgt ctgcacatga cgttcgacgc ctatcattcg gacttcaacg aattgcagcg
      721 catcgcgcgt ctggaatatc cgctggcatg gggcgccaac catctggagc cgggctatac
      781 gaccgacaat gaattcgtaa ccagcggcac gttcgacgac gtcaagacgg tggtggagaa
      841 ctatgtcaac cagcgcaagg acaagctcga ctcctacggc tggaacacca cctacgacct
      901 gaacgacaag tggtccctga tggccgattt gtcggaatcg aaggccgacc gcgtcgacgt
      961 gcaggtcgaa tcgaccgccg gcaccggccc caacggttcg ggcgcgaccg atacggtcag
     1021 cttcacccag cagggcttcg gcctgaccag cctgaagaac gcgatcgact attccgactt
     1081 cgacaccatc ttcctgaccg atccgggcgg gtggggcggt ctggcgccga accgcgccgg
     1141 ttatgcgcgc gagccgcatg tgacggacaa gatcgactcg atcaagctgg cggcgacgcg
     1201 gcatttcggc ggcgggttcc tcagcgacgt gtcgttcggc tacgaccaca ccgagcatcg
     1261 caagcacaag gagggcgtcg aaggctatct gggcctcgac ggcgtctccg aggcgtcggt
     1321 gccggatcag taccgcaccg gcacgaccga caccggcttc ctcggcagcg cgggcggcat
     1381 gatctcctac gacgcgctgg ccctgtatga gagcggcttc ttcaagttct acgaagaagt
     1441 ggccgccgcc gccgtgcaaa agacgtggac ggtgaacgaa aagctcgaca ccttctacgt
     1501 caaggccgac atcgatacga ccctgtgggg cgtgccgctc accggcaata tgggcctgca
     1561 atcggttcac gccgaccagc gcgcgacgtc cttctacacc gacggcaccg ccgacggggt
     1621 gacgccgcaa tcgggcggcg ccagctatac cgacatcctg ccgaccctga acctgaactt
     1681 ccagctggcc gacgacacca cgctgcgctt cggcgccggc acgaccatcg cgcgcccgcg
     1741 catggacgac atggcggcgg gcaccagcta ctcgacctcg gtcaacacca atccgtcggt
     1801 gatcggcacg cagaaatatt actggagcgg cggcggcggc aatccggccc tgcgtccgtg
     1861 gaaggccaac aatttcgacc tgtcggtcga gcattatttc ggccgcaagg gctatatttc
     1921 gggcgcgctg ttctacaagg atctgaccac cttcatctac agccagaccc tgctgaagga
     1981 ctataccggc gtcgatccgt cgggcgcctg ctggtcggac gcggcgcatt cggtcaaggt
     2041 ggtcgacgcc gacaagacca gcccctatta cggccagtgg gtctgcgcca tggccgacgc
     2101 caaccgcatc ggcacgacca cggctcaggc caacggtcat ggcggctata tcaagggcgt
     2161 cgaactgacc gtctccatgc cgggcgaact gatcgcgtcg tggctggacg gcttcggcgt
     2221 gatctggagc gcctcgtaca acgcctcgtc gatcaacccg tccgggaaca acgcctatga
     2281 cgtgccgggc ctgtcgaaag aggtgatgaa caccacgctg tactacgaaa agcacggctt
     2341 ctcggcgcgg atcagcaacc gctatcgcgg cgacttcctc ggcgaggtgc cggactacac
     2401 caacagcctg agcaacacct gggtgcattc ggaaaacatc gtcgacgccc agatcggcta
     2461 cagcttcgat tccggtccgc tcaagaacct gtcgatcaac atctccgggt cgaacctgac
     2521 caacgagccc ttctacacct atcagggcaa ggggctgccg gatcatatcc tgcgctatga
     2581 aaagtacggc tcgacctacc tgttcggcgt cagctacaag ttcttctaag gcgtacgacg
     2641 acaatggaaa cgcccgccgg tcgctccggc gggcgttttc cctgttcgcg caaaatctct
     2701 tcgcaaattt acaggtttcg ggctaaacag cgcctatgcc cgatcaagac gcctcagcca
     2761 aaacctcccc gttcggtttc cgcgatgtcg acgcgcgcga gaaagtcgcg atggtgcatg
     2821 gcgtgttccg caatgtcgcc tcgaaatacg acctgatgaa cgacgtcatg tcgggcggcg
     2881 tgcatcatct gtggaaggac gcggcctgtg cgcggctcaa tccgcagccc ggcgaggtca
     2941 tcgtcgattg cgcgggcggc accggcgaca tcgcgcgccg tctggcgaaa ctggcgcggc
     3001 gggccagggc gcggcgcgag gcgcgcggct tcgaggccgc cgacgccgac atccgcatca
     3061 tcgactataa cgaagccatg atcatggccg ggcgcgaaaa gtccgaacgc aaggctcttt
     3121 acgagccgga aatctcatgg tcggtgggcg acgcgatgaa cctggccctg ccggacgatt
     3181 gcatcgacgc ctacatcatc tcgttcggca tccgcaacgt cgccgacgtg caggcggcgc
     3241 tgggcgaggc gtatcgcgtg ctgaaacccg gcgggcgctt cttctgcctc gaattctcgc
     3301 atcccgaatc gccggtggtg gagaaggtct atgaggccta cagcttcaaa ctcatcccgt
     3361 tcatgggcaa gatcgtcgcc aacgaccgcg acagctatca gtatctggtc gagagcatca
     3421 agcgcttccc cgatcaggag acgttccgcg gcatgatgca gaaggccggt ttcgaccgcg
     3481 tgacctatac caactattcc ggcggggtct gcgccctgca cgaaggctgg aagtaggctg
     3541 gtttcatggc ccatctcggt tcatggctgc ggctgacgcg cgcggggttc accctgttgc
     3601 gctacgacgc gctgatcccg aaggaattgt cgccgctgct gccgccgatc ccgcgctttt
     3661 tcgccgggct tctgcgcggc ctgttcgcgc gccgcagccg cctgcgcgtc ggcgaacgct
     3721 acgcgctggc cttcgagcgg ctcggcccgg ccttcatcaa gctggggcag gtgctgtcga
     3781 cgcgcggcga cattttcggc gagcgcttca tcgcggacct cacccacctc aaggacagcc
     3841 tgccgccctt cgcgaagacc gaggccgagg cggcgatcct cgacgcgttc ggcaagccgg
     3901 tcgcggcgat tttcgccgat ttcggcgagg tgatcggctc ggcctcgatc gcgcaggcgc
     3961 accgcgccgc cctgctggac gggcgcgagg tcgcggtcaa gatcctgcgc cccggcatcg
     4021 agctgaccgt ggcgcgcgac gtcgacatgc tgttgctggg cgcgcggctg gtggcggccc
     4081 tggtcccggc ctcgcgccgt ctggagcccg aagccttcgt cgccacggtg gccaagtcgc
     4141 tgcaactgga gctggacctg cggctggaag cctcggcctg cgacgagatc ggcaagatca
     4201 tcaatgacga gccgtggatg aaggcgcccg ccgtggtctg gagccacgtg tcgcgccgag
     4261 tcatcgtcac cgaatgggcc gacggcgtgc ctctgtcgcg cccggaggcg ctggacctgc
     4321 ccggcctcga ccgcaaggcg ctggccgaca acctgatccg cgccttcctg tcgcaatcgc
     4381 tcgatcacgg cgtctttcac gccgacctgc acgagggcaa cctgttcgtc gccgctccgg
     4441 cgcggatcac cgccatcgat ttcggcatca tcggccgcct gcgcccgaag gagcggcgct
     4501 atctggccga gatgctgtgg ggcttcctgc gccgcgacta caagcgcgtc gccgaggtgc
     4561 atttcgaggc cggctacgtg cccgcgcacc agaatgtcga ggcgttcgcg caggccctgc
     4621 gcgcggtcgg cgagccggtg ctgggccgtc aggccagcga cgtctcgatg ggccgtcttc
     4681 tgacgcaact gttcgagatc acggcccagt tcgacatgca cctgcgcccg gagctggtgc
     4741 tgctgcaaaa gaccatggtg tcggtcgagg gcgtggcgcg gcgcatctat cccgaacaca
     4801 atctctggga ggccgcgcgc ccggtggtga aggcgtggat ctcgcgcgag ctgtcgccgg
     4861 tggccgaggc gaagaagctg ctggaaaccc tgatccggcg gctcaacgac gatccgtccg
     4921 agcgcatccg cgacgccctg accgcgcgcg actccctgcg cgccgacctg cgccgctcgc
     4981 aggggctggc cctggtcgcg gtcatcgtgt ccgccctgtc gctgggcgtc atgatctggc
     5041 tggcgctgtc gaaatagcgg cggacgccgg gggcgcattt ttgtcgcata tgcaaccatg
     5101 cgcgccattt acgccgcgcg ccaaagcgtc taggactccg gttgagtttg tcacgcggct
     5161 tcggtggtgt tcatggcgat tggcgttttc gattccggca tcggcggtct gtccatccat
     5221 cgcgcgctgg tcgaggtcat gccgcgggcc gatttcatct atctggccga ccagaagaac
     5281 accccctatg gcgacaagcc gggcgaggcg atcgtcgagc tgacgcgcgc ggggtgccag
     5341 accctgttcg acgcgggcgc ctcgctggtc gtgctggcgt gcaacacggc ctcggcggtg
     5401 gcgctgcggc ggctgcaatc gacctggctg gtggactatc gcaagacctg cggacggccg
     5461 gtcaacgtgc tgggcatcat cgtgccgacc atcgaggcgg ccaccggcct gccgtgggaa
     5521 tacgcggccg accggcgcgg ccccaagatc gaaaagctgg acgtgatcgg cgtcttcgcc
     5581 accgccgcca cggcgcgctc gcgcgtgtac gagatcgaga tcgacaagcg ccgcgacgat
     5641 ctggccgtgt tcaccgtcgc ctgttcgggg ctggccgaca tgatcgaggg cgccgcgccg
     5701 cgcgcgcgga tacgcgagct gatcgcctcc tacgcgcagg cgctgaggac gcgcatcggg
     5761 cgctggcccg accgcgccat cctcggctgc acccattacg agatcgtcgc cgacctgttc
     5821 cgcgaggtct tgccggaagg gacggaactg atcggccagc ccgcgccggt ggccgcggct
     5881 ctggccgact atgtccgccg ccatcccgaa tacgcgaccg gccggggcgg cgggcgccgt
     5941 tttctgacca ccggcgatcc gggcgcgcag ccgcccctgg ccgaagcctt ctggggcggc
     6001 ccgctggcct tcgagccggt tcagaccaag gcggttcagg gcttctgata gacccgcacg
     6061 taatcgacca ccatcgattc cggcagggcg ccgtcgtcca cgggtccggc ccagcttccg
     6121 cccagcgcga ggttgagcag caggtactgc ttttcgtcga acggccactg gtccttgccg
     6181 gtatgggcgt tggcgtaggt ctggtagatc acgccgtcga cgccgatgtc gatctcgtcc
     6241 gccgtccagg tcaattcgta attatggaag ttgccgcagg cgtcgctgat tgtggtgtgc
     6301 gcgccgtcgc cgttgtgcgc ttgattggag gtgtcgtgga tcgtgccgta gatcgtgttc
     6361 ggatccttgc ccagttgctc catgatgtcg atctcgccgt ccgccggcca tgagccgccg
     6421 tcgcccagca tccagatcgc cggccacgcc ccggcgccgc acggcaattt ggcgcgcacg
     6481 tcgacgaagc cgtacagcca cgacgacttg ccgcgcgtga cgatgcgcgc cgaggtgtag
     6541 gcctgtcccg cgtaattggg ctggcccgtc gcggcttcct tgcgcgcggt caggtacaga
     6601 tagtcgccgt cctgacgctc gttgtcgaga tcgcccgccg tgtaatattc cagttcgcca
     6661 ttgcccggat cgaaggtgtt ggcgttggtg tcgtcgctcc atttgccgga gtcgggcagg
     6721 ccggaaccgt cgaattcatc gttccagacc agcgtgtaac ccgccggaac ccaggccgaa
     6781 tcggtgatcg gccccgccga agacgaagat gatgatgacg acgaagatga agacgacgaa
     6841 gacgaagacg aagacgagga tgaggacgaa gacgacgaag atgatgagga cgacgaact
//
LOCUS       DASVLL010000608         5955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_152769,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000608 DASVLL010000000
VERSION     DASVLL010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152769"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="bioA"
                     /locus_tag="VF402_09975"
     CDS             <1..1065
                     /gene="bioA"
                     /locus_tag="VF402_09975"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX7800667.1"
                     /translation="QVIFAGFTHEPAERLAQKLVALTGPRLTRVFFSDSGSTAVEAAL
                     KMALGFHRHSGRPRSGIAALEHAYHGDTFGGMSVGARGVFNAAYEDLLFDVHRLPFPA
                     AGREQATLDAFEALARSGAIAALLLEPLVLGAGGMLMYGPDVLRGLHEICGRYGVLFI
                     ADEVMTGWGRTGTRFACDQAGIVPDIMCLSKGLTSGSLPLAVTMASEAVYQAFYSEDR
                     ARAFFHSSSFTGNPLACAAACAALDIWDEEPVTQRIAHLHAMQARMLRVFAARPDVEN
                     PRHTGTIMAFDVRADDPGYLSAIGPKLHRFCLDAGVLIRPLGQTVYLLPPYCLSQEDM
                     DVITDALTRALDALRLRRPE"
     gene            1037..1672
                     /locus_tag="VF402_09980"
     CDS             1037..1672
                     /locus_tag="VF402_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800668.1"
                     /translation="MLSDFGGLNDALSAPFAAFLRAAVAGRDGHARFLNLLSLLEHIG
                     SRKIMLSQMKGVLTQDILKHLAEETRHAFFFKREAEKLAGRTIDGYAPDDTLAAASGR
                     LYFGRLDAGLTRGLAGEAGYLWVSLIVELRALWVYRLYQQALDAAGLKLSLRGVIAEE
                     DRHLEEMIARLDALGFDADARLAPALALEQTLFRTLLAALQAQAAPEPNPA"
     gene            complement(1680..2501)
                     /locus_tag="VF402_09985"
     CDS             complement(1680..2501)
                     /locus_tag="VF402_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800669.1"
                     /translation="MRNFSLSLRGPGGTGQHAYVQLVDHDAQGGAFRIGGELGPYTIV
                     AATGQAVLAQQGDTRPPGMSNLNSPWVAVNPGAHGEMVNFQLQINPDIVPAGVYDKVL
                     DLRVACPSGHGYTYTIVPAVVEIHVAVDNVVKLVGGHNARLDFGDLAIDGPPSKAQEL
                     VGLRASGAFNIAVRSEGGVIAGANSGVMMRENAGGLTDADRIPYRVNVQNLPMNGAEA
                     HYRCGGAVGAISIAVQTNPSPGAGKHAGTYRDILHVTLTPDFYGQVVDEGRCKAM"
     gene            complement(2645..5221)
                     /locus_tag="VF402_09990"
     CDS             complement(2645..5221)
                     /locus_tag="VF402_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800670.1"
                     /translation="MKAAKHGRRLVRLVGCMASLSGGAHAGTWLAATQASAVDTAKPP
                     APTMSPAQTSTSAPASGAAPASIKVRGGLVVSNRLNTTDHALRLTAPLSSVDRKLGDV
                     DILIEPDDSLRVSKASLIAALAPILSEDRQKALSDAATTDGYLSLADMSALQMPMVWD
                     PNSLRLSITVTGGARSVQQLNIADEDQIGASIPMARTSAYLNMRTAVSSYASSGQNSD
                     VNLDLEGGLRVHGLLFQNEAAYRPLVLGGQQEFVREGTRVIWDDRKRLMRFTFGDQQP
                     LNRGFQAFSDMAGIGVYRTYEDLDPLRNARPTGTSAFTLQRPSMVETLVNGVPTAQFQ
                     LNPGRYNLNNIPYTQGANNVTIRVTDDLGMQQDINFSLFFDRQLLAKGLSEFAFYAGV
                     PSDISTGRIEYMHNRYIVSGYYRRGLTENLTLGGNFQSDNRSGMAGGEALWASPIGSF
                     GGDVAYSWASGGQSGYAINTSYESTLTRDNIHSTSFGLSLTMQSANFMPVEIYQQPSV
                     YKYQVSANLSHGFSINTTAFAQLDYSKGRGLEPDLASAGLGVTQRITDRIYLSAEARY
                     EKTAFHNGYGVRVALNYRFGRHTTATAISDTVNDLTQLSLNSSHGHGVGAWTANADVI
                     RQTDGDVVNANLNYDANRAEIILNQSTPFDTTQGSSLSGQTTLSVGTALVMADGHWGI
                     GRPIYDSFVMAMPHRTLKSRINLDMTPEGVVARSGMFGPAVDPEIGSYMPRNVTYTAP
                     DAPAGYDVGSGSVRVLPAYRSAYVIVVGSDYNVMAYGRMYFANGKPIPLLAGQATELA
                     HPERAPIQIFTNAQGRFSLTGARPGRWRVDMPANPAVSFIIDIPEGNNLVNLGDLKAS
                     QP"
     gene            complement(5208..>5955)
                     /locus_tag="VF402_09995"
     CDS             complement(5208..>5955)
                     /locus_tag="VF402_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEX7800671.1"
                     /translation="LGVGAIVMAAAGMAVTSARAMGVSPFVIDLNKNGQGMTATITVE
                     NNFKTPLPVEMRVEALDFDANGVVETHKDPGDLIVFPMQAIIEPGQSRVFRVQYAGAP
                     PTEKSKHYYVTAAQLPVDLGKDASEVQVVYAFNVLLSIPVPGAKAHLRVTKGAIGHSD
                     KDPSSFFPILTVENAAITYGYFKNGKLKLTQTNSAGKVVFAKTFDATALEHIVGWGLI
                     GPGQTRDLPLNIPLPSGEGQLKAEYSYEGG"
BASE COUNT          967 a         1958 c         1975 g         1055 t
ORIGIN      
        1 caggtgatct tcgcgggctt cacgcatgaa ccggcggagc ggctggcgca aaagctggtc
       61 gcgctgaccg gcccgcgcct gacgcgcgtg ttcttttccg acagcggctc cacggcggtc
      121 gaggcggcgc tgaaaatggc gctcggcttt caccggcaca gcgggcggcc gcgctcaggg
      181 atcgcggcgc tggaacacgc ctatcacggc gacactttcg gcggcatgtc ggtcggggcg
      241 cgcggcgtgt tcaacgccgc ctatgaagac ctgctgttcg acgtgcatcg cctgcccttt
      301 cccgccgccg ggcgcgaaca ggcgacgctc gacgcgttcg aggcgctggc gagatcgggc
      361 gccatcgccg cgctgctcct cgaaccgctg gtgctgggcg cgggcgggat gctgatgtac
      421 gggccggacg tgctgcgcgg cctgcacgag atatgcggcc gctacggcgt gctgttcatc
      481 gccgacgagg tgatgaccgg ctggggccgc accggaacgc gcttcgcctg cgatcaggcg
      541 ggaatcgtcc cggacatcat gtgcctgtcc aaggggctga cctccggctc gctgccgctg
      601 gccgtgacca tggccagcga ggcggtctat caggctttct acagcgagga ccgcgcgcgc
      661 gccttctttc attcctcgtc cttcaccggc aatccgctgg cctgcgccgc cgcctgcgcc
      721 gcgctcgaca tctgggacga agagccggtg acgcagcgca tcgcgcatct gcacgccatg
      781 caggcgcgga tgctgcgcgt cttcgcggcc cgcccggacg tcgagaatcc gcgccacacc
      841 ggcacgatca tggccttcga cgtgcgcgcc gacgatccgg gctacctgtc ggccatcggc
      901 ccgaaactgc accgcttctg tctcgatgcg ggcgtgctga tccgcccgct gggccagacg
      961 gtttatctgc ttccccccta ctgcctgtct caggaggaca tggatgtcat caccgacgcc
     1021 ctcacgcgcg cgctcgatgc tctcagactt cggcggcctg aatgacgccc tgagcgcccc
     1081 gttcgccgcc ttcctgcgcg ccgccgtcgc cgggcgcgac gggcatgcgc gtttcctcaa
     1141 cctgctgtcc ctgctcgaac acatcggcag ccgcaagatc atgctgtcgc agatgaaggg
     1201 cgtgctgacg caggacatcc tcaaacatct ggccgaggaa acgcgccacg ccttcttctt
     1261 caaacgtgag gccgaaaagc tggccgggcg gacgatagac ggctacgcgc ccgacgacac
     1321 cctggccgcg gcgtccgggc ggctttactt cggacggctc gacgcgggcc tgacgcgcgg
     1381 tctcgcgggc gaggcgggct atctgtgggt gtcgctgatc gtcgagctgc gcgccctgtg
     1441 ggtctatcgc ctgtaccagc aggcgctgga cgcggccggg ctgaagcttt ccttacgcgg
     1501 cgtgatcgcc gaggaggacc ggcatctgga ggaaatgatc gcgcggctgg acgcgctcgg
     1561 cttcgacgcc gacgcgcgcc tcgcccccgc gctggcgctg gagcagaccc tgttccgcac
     1621 cctgctggcc gcgttgcagg ctcaggccgc gccggaaccg aacccggcct gagccgccgc
     1681 tacatggcct tgcatcgccc ctcgtccacg acctgcccgt agaagtccgg ggtcagggtc
     1741 acgtgcaata tgtcgcgata cgtgccggca tgcttgcccg cgcccggcga cgggttggtt
     1801 tgcacggcga tggaaatggc cccgacggcg ccgccgcagc gatagtgcgc ctcggcgccg
     1861 ttcatcggca ggttctgcac gttgacgcgg tagggaatgc ggtccgcatc cgtcaacccg
     1921 ccggcgtttt cgcgcatcat gacgccgctg ttcgcgccgg cgatgacgcc gccttcggag
     1981 cgcacggcga tattgaaggc gccggacgcc cgcagcccga ccagttcctg cgccttcgac
     2041 ggcggaccgt cgatggccag atcgccgaaa tcgagccgcg cattgtggcc gccgaccagt
     2101 ttgacgacat tgtccacggc gacgtggatt tcgacgaccg cgggcacgat ggtgtaggta
     2161 tagccatgtc cgctggggca agcgacccgc aggtccagca ccttgtcata gacccccgcc
     2221 gggacgatgt ccgggttgat ctgcaactga aaattgacca tctcgccgtg cgcgcccgga
     2281 ttcaccgcca cccagggcga gttgaggttg gacatgccgg gcgggcgggt gtcgccctgt
     2341 tgcgccagca cggcctgacc ggtcgccgcg acgatcgtat aggggccaag ctctccgccg
     2401 atgcggaagg cgccgccctg cgcgtcgtgg tcgacgagct gcacataggc gtgctggccc
     2461 gttccgcccg gaccgcgcag gctgagggaa aagttgcgca ccgcgcctcc ggctccgaag
     2521 ggatcgaaac gctccacatt gacgaaggga tgcgaagtcg aatctatgca ggctgacgcg
     2581 gggcaggccg ccagggcttc cagggcgacc aacaggaaaa gcatgattgc gcgcatgtcg
     2641 gttctcacgg ttggctggct ttcagatcgc caagattaac gagattattc ccttccggga
     2701 tgtcgatgat gaacgacacc gccggatttg caggcatatc gacgcgccaa cgcccggggc
     2761 gcgcgccggt caagctgaag cgaccctggg cgttcgtaaa gatctggatc ggcgcccgct
     2821 ccgggtgcgc cagttcagtg gcctgacccg ccaacagcgg aatgggcttt ccgttggcga
     2881 aatacatgcg gccatacgcc atcacattat agtccgaacc cacgacaatg acataggccg
     2941 aacgataggc cggcagcacc cgcaccgacc cggagccgac atcgtagccc gcgggcgcgt
     3001 ccggcgcggt ataggtgacg ttccgcggca tgtacgagcc gatctccgga tcgaccgcgg
     3061 ggccgaacat cccgctgcgc gccaccacgc cttccggcgt catgtcgagg ttgatccgcg
     3121 atttcagcgt gcggtgcggc atcgccatga cgaagctgtc gtagatcgga cgcccgatgc
     3181 cccaatgccc gtccgccatg accagcgccg tgccgacgct gagggtcgtc tggccgctca
     3241 ggctcgatcc ctgcgtcgtg tcgaagggcg tggactggtt caggatgatt tcggcgcggt
     3301 tggcgtcgta gttgaggttg gcgttcacga catcgccgtc cgtctgacgg atcacgtccg
     3361 cgttggcggt ccaggcgccg acgccgtgcc cgtgcgagct gttcagggac agttgcgtca
     3421 ggtcgttgac cgtatcgctg atcgccgtcg cggtggtatg ccgcccgaag cggtagttga
     3481 gcgcgacgcg cacgccgtag ccgttatgga aggcggtctt ttcataccgg gcttccgccg
     3541 acaggtagat gcggtcggtg atgcgctggg tcacgcccag accggcggac gccagatccg
     3601 gctccagccc ccgccccttg ctgtagtcga gctgggcgaa cgcggtcgta ttgatcgaaa
     3661 agccgtgcga gagattggcg gagacctgat acttatagac cgacggctgc tggtagatct
     3721 ccaccggcat gaaattcgcc gactgcatcg tcagcgacag cccgaacgag gtcgagtgga
     3781 tattgtcgcg cgtcagcgtg ctttcgtaac tggtgttgat cgcatagccc gactggccgc
     3841 ccgacgccca tgaataggcg acgtcgccgc cgaacgaccc gatcggcgaa gcccacagcg
     3901 cctcgccccc ggccatgccg gaccggttgt ccgactggaa attcccgccc agggtcaggt
     3961 tttccgtaag gccgcggcgg taatagccgg aaacgatata gcggttgtgc atatattcga
     4021 tccggccggt ggagatgtcc gacggcacgc ccgcgtaaaa ggcgaattcg gacagcccct
     4081 tggccagcaa ttgccggtcg aagaacagcg agaaattgat gtcctgctgc atgcccagat
     4141 cgtccgtcac gcggatggtg acgttgttgg cgccctgggt atagggaata ttgttgaggt
     4201 tgtagcggcc gggattcaac tggaactgcg ccgtcggcac gccgttcacc aaggtctcga
     4261 ccatggaggg ccgttgcagg gtgaaggcgc tggttcccgt cggacgcgcg ttgcgcagcg
     4321 ggtccagatc ctcataggtg cggtataccc cgatgcccgc catgtcgctg aacgcctgaa
     4381 aaccgcgatt caggggctgc tggtcgccga aggtgaagcg catcaggcgc ttgcggtcgt
     4441 cccagatgac gcgcgtgcct tcgcgcacga attcctgctg gccgcccagc accagcgggc
     4501 ggtacgccgc ttcgttctgg aacagcaggc catgcacgcg caggccgcct tcgagatcga
     4561 gattgacgtc gctgttctgg cccgaactcg cgtaggaact gacggccgtg cgcatgttca
     4621 gataggccga tgtcctggcc atcgggatgg aggcgccgat ctggtcctcg tcggcgatgt
     4681 tcaactgctg caccgatctg gcgccgcccg tgacggtgat cgacaggcgc aggctgttcg
     4741 gatcccaaac catcggcatc tgcaaggccg acatgtccgc cagcgacaga taaccgtccg
     4801 tcgtcgccgc gtcgctcagg gccttctggc ggtcctccga caggatgggc gccagcgcgg
     4861 cgatcaggct ggccttgctg acgcgcaagg agtcgtccgg ctcgataagg atgtcgacat
     4921 cgcccagttt acggtcgacg gagctcaggg gcgcggtcag acgcaaggcg tggtccgtgg
     4981 tgttcagccg gttgctgacg accagaccgc cgcgcacctt gatcgaggcc ggggcggcgc
     5041 ccgaagccgg agccgacgtt gacgtctggg caggactcat cgtcggcgcg ggcggtttcg
     5101 ccgtgtccac ggcgctggcc tgcgtcgccg caagccacgt ccccgcatgc gcgccgcccg
     5161 acaggctcgc catgcagccg acaagacgaa ccagccgtcg cccgtgctta gccgccttca
     5221 tagctgtact cggctttcaa ctggccttcg ccgctgggca gcggaatatt cagcggcagg
     5281 tcgcgcgtct ggcccggccc gatcaggccc cagccgacga tatgctccag cgccgtggcg
     5341 tcgaaggtct tggcgaagac gacctttccc gcgctgttgg tttgggtgag cttcagcttg
     5401 ccgttcttga aatagccgta ggtgatggcc gcgttctcga cggtcaggat cggaaagaag
     5461 gacgacggat ccttgtcgct gtgtccgatg gcgcccttgg tgacgcgcag atgcgccttg
     5521 gcgcccggca ccgggatcga aagcaggacg ttgaaggcgt aaacgacctg aacttcggac
     5581 gcgtccttgc cgagatcgac gggcagttgc gccgccgtca cgtaataatg cttggatttt
     5641 tccgtcggcg gcgccccggc gtattgcacg cgaaagaccc ggctttggcc cggctcgata
     5701 atggcctgca tcggaaagac gatgagatcg ccgggatcct tatgcgtttc gaccacgccg
     5761 ttggcgtcga aatccagagc ctcgacgcgc atctccacgg gcaggggcgt cttaaaattg
     5821 ttctccaccg tgatcgtggc ggtcatgccc tgaccgttct tgttcaggtc gatgacgaac
     5881 ggactcacgc ccatggccct ggcgctcgtt acggccatcc cggccgccgc catgacgatc
     5941 gcgccgacgc ccagc
//
LOCUS       DASVLL010000609         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_527457,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000609 DASVLL010000000
VERSION     DASVLL010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527457"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..221
                     /locus_tag="VF402_10000"
     CDS             81..221
                     /locus_tag="VF402_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800672.1"
                     /translation="MRSKTADSFVVCLQRAPFRAGADFEAFDRIMARSGGQPPEGDDR
                     LP"
     gene            276..1553
                     /locus_tag="VF402_10005"
     CDS             276..1553
                     /locus_tag="VF402_10005"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX7800673.1"
                     /translation="MDDHSRQSLEQLMTGMAEKARLGAEALRLATPETRTKAILEAAR
                     AIRAREADILAANARDKQFARDKGLTDAMIERLILNPARVEAMAGGLEEVAALPDPVG
                     RELARWTRPNGLDIARVATPIGVIAIIYESRPNVTADAAALCVRSSNAAILRTGSEAL
                     NSALAIYAAFQDGFRAAGLPDDCVQLVPTSDRDAVGLILSGLDKTINLIIPRGGRSLV
                     ERVQKEARAPVLAHLEGLNHTYIHSGADPKKALEVTLNAKMRRVSVCGATETLLIDEA
                     VAADLLPGIAAALEAKGCELRGDEGARALVPSMTPATEDDWRTEYLLPILSVKVVRDE
                     AEAIQHIRTYGSGHTEAIVTEDAAAAETFLNEVDSAIVIWNASTQYADGGEFGLGAEI
                     GISTDRLHARGPVGAEQLTTFKYVVRGDGQTRP"
BASE COUNT          292 a          547 c          548 g          256 t
ORIGIN      
        1 cgaaatctcg aaatatggcc gcgttcatat cgcgtttaac cgctctgccg ggttttcgtc
       61 cggccgccta cgccacggcc atgcgctcga agacggccga cagcttcgtg gtatgcttgc
      121 aacgcgcgcc gttccgcgca ggcgccgatt tcgaggcgtt cgaccgcatc atggcgcgct
      181 ccggcggcca gccgccggaa ggcgatgatc gtctcccata aaacggtcgt aattcccgaa
      241 ggctgcgcta tataaggctt cgaaaggata ttctcatgga cgatcacagc cgccaatcgc
      301 ttgagcaact gatgaccggt atggccgaga aggccaggct gggcgccgag gcgttgcggc
      361 tggcgacgcc ggagacgcgg accaaggcca ttctggaagc cgccagggcc atccgcgcgc
      421 gtgaggccga cattctggcc gccaatgcgc gggataagca attcgcgcgc gacaagggcc
      481 ttaccgacgc gatgatcgag cggctgatcc tcaatccggc gcgcgtcgag gcgatggccg
      541 gggggctgga ggaggtcgcg gcgctgcccg atcccgtcgg gcgcgaactg gcgcgctgga
      601 cgcggccgaa cgggctggac atcgcacgcg tggctacgcc catcggcgtg atcgcgatca
      661 tctatgagag ccgtccgaac gtgacggcgg acgcggcggc gctgtgcgtc cgctccagca
      721 acgcggcgat cctgcgcacg gggtcggagg cgctcaactc ggcgctggcc atctatgcgg
      781 cgtttcagga cggcttccgc gcggcggggc tgcccgacga ttgcgtgcaa ctggtgccga
      841 cctcggaccg cgacgcggtg gggctgatcc tcagcgggct ggacaagacg atcaacctga
      901 tcattccgcg cggcgggcgc tctttggtcg agcgcgtgca aaaagaagcg cgcgccccgg
      961 tgctggccca tctcgaaggg ctgaaccaca cctatatcca ctctggcgcc gatccgaaaa
     1021 aggcgctgga ggtcacgctg aacgccaaga tgcgccgcgt ctcggtgtgc ggcgcgaccg
     1081 aaaccctgct gatcgatgaa gcggtggcgg cggacctgct gccgggcatc gccgccgcgc
     1141 tggaagcgaa gggctgcgag ctgcgcggcg atgaaggcgc gcgcgcgctc gtcccgtcga
     1201 tgacgcccgc tactgaggac gactggcgca ccgaatacct gctgccgatc ttgtcggtga
     1261 aggtggtcag ggacgaggcc gaggcgatac agcatatccg aacctacggc tccggccaca
     1321 ccgaggcgat cgtcaccgag gacgccgccg ccgccgaaac cttcctcaac gaggtcgatt
     1381 ccgccatcgt gatctggaac gcctcgacgc aatacgccga cggcggcgaa ttcggcctcg
     1441 gcgccgagat cggcatctcg accgatcgcc tgcacgcgcg cggccccgtc ggcgccgagc
     1501 agctcaccac cttcaaatac gtggtgcgcg gcgacggcca gacccggccg taaaagcctt
     1561 aatcccgcct ttacgccgcg tcacataatc ttcgccgaag cgcgttaatc acgccattct
     1621 cctgaacggc gcgcgcgtgc tac
//
LOCUS       DASVLL010000610         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_364134,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000610 DASVLL010000000
VERSION     DASVLL010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364134"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VF402_10010"
     CDS             complement(<1..569)
                     /locus_tag="VF402_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX7800674.1"
                     /translation="MFLWSLDRATDLRYAHPWLLYLLPVAGFLIGLVYLWKGRPAEGG
                     NNLILEQIHEPGGGVPLRMAPFVLVATVLTHLFGGSAGREGTAVQMGGSLASGVGKLF
                     KLDADDVRILLMAGIAAGFGAVFGTPLAGAVFALEVLTVGRVQYAALVPCLAASLVAD
                     YVCRAWGIHHVEYHIGYMAGLTHVAFDPV"
     regulatory      complement(735..812)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(895..1098)
                     /locus_tag="VF402_10015"
     CDS             complement(895..1098)
                     /locus_tag="VF402_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800675.1"
                     /translation="MPLILAISIVFGLIGGIMAYIITWLEYRRHKLKGWRLIQPCLST
                     AGGVFLFFVALGGLFGFFLLKTL"
     regulatory      1198..1258
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1325..2521
                     /locus_tag="VF402_10020"
     CDS             1325..2521
                     /locus_tag="VF402_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800676.1"
                     /translation="MIAPQDDVRKSRMAAMAMRFVLLTGAVSLFSDFTHEGARSLLGP
                     YLALLGASAFIVGLVTGFGEFLGYALRLVSGRWADATGRFWPITILGYVVQMAAVPAL
                     ALTHTWPQAAALIVLERVGRAIRVPPRDVMLSHAAHHIGGYGWTFGIHQACDQTGALL
                     GPLLLALVMGWRGDYHFAFAVLLVPATINIALLLISRRLYPNPEAMEVHPPRTAPGDR
                     FSPAFWVYMAGASLVALGFADYPLIAYHFVHHKIAPVGWIAGFYAVAMAVSGGGSLVF
                     GRLYDRFGFRMLVVLTIVAALFAPLAFFGDFWLALIGAALWGLGMGVHESIIPAAVTP
                     LVPPSKRASAFGLFTAVYGAFWFAGSAVIGFLYDRTLSGTVIFCVATQMLAAPVFMWL
                     AGRRSP"
     regulatory      2622..2684
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2766..>3016
                     /locus_tag="VF402_10025"
     CDS             2766..>3016
                     /locus_tag="VF402_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800677.1"
                     /translation="MTGQVEYLTQDAIGEPHGSWRGMPEIPRLKRSYTTLLQALSHAH
                     YGGLKMTLPDGALLHYHGAERGETAHIHILDWKAFDQIIA"
BASE COUNT          520 a          978 c          972 g          546 t
ORIGIN      
        1 atgaccggat cgaaggcgac atgggtcagg cccgccatat agccgatatg gtattcgaca
       61 tgatggattc cccacgcccg gcagacatag tcggcgacca gcgaggcggc caggcacggc
      121 accagcgccg cgtactgcac ccggccgacg gtgagcacct ccagcgcgaa caccgccccg
      181 gccagcggcg tgccgaacac cgcgccgaac ccggcggcga tcccggccat caaaaggatg
      241 cgcacatcgt cggcgtcgag tttgaacagc ttgccgaccc cgctggccag actgccgccc
      301 atctgcacgg cggtgccttc gcggcccgcc gagccgccga acaggtgggt cagcacggtg
      361 gcgaccagca cgaacggcgc catgcgcagc ggcacgccgc cgcccggctc atgaatctgc
      421 tccaggatca ggttgttgcc gccctcggcg ggccggccct tccacagata gacgaggccg
      481 atcagaaacc ccgcgacggg caggaggtac aacagccacg gatgcgcgta ccgcaggtcc
      541 gtcgcccggt ccagcgacca caggaacaca gcgcacgccg accccaccgc aaccgccatc
      601 ggagccagaa gcagggtcca gcggccgagg acgacggcaa aacgcagacg ggaggatgag
      661 acggaagcgt aaatatcctc ggcgcgaaga cgccggaagt tcataacaga cacgattcta
      721 ccttttgcca acacaataag gtaggaatca tcagcccgtc gaggagcggt tcggcgcata
      781 aggcccggtg gaaatccatc accgttaccg cagcgcaata gcggcgtttt gccccagcgt
      841 caagacgcca atccgcacgt agggtgacaa attgttgcaa tgcggcgcag acggtcagag
      901 cgtcttcaac aggaaaaagc cgaacaggcc gcccaaggcg acgaagaaaa gaaagacgcc
      961 gcccgccgtc gacaggcagg gttggatcag ccgccaccct ttgagcttat gacgccggta
     1021 ttccagccag gtgatgatgt aggccatgat cccgccgatc aggccgaaga cgatcgagat
     1081 ggcgaggatc agcggcatga cggttcggct ttcgggcata atcgtggaga cgcgacacac
     1141 tgaaaacggg cgggaagcgc gcatccggcg ttgccttgcc gcccgcccat gctaagctgg
     1201 ctatccggga atggagtttc cctttaaccg ccgtctggct gatgactcct gcatgcgcgc
     1261 cttgggccgc agcgggaggt cgtgtccttc gcgccgccct gggcgatgga gaaggacgaa
     1321 tgcgatgatc gctccgcaag acgatgtccg caagagccgc atggccgcca tggccatgcg
     1381 cttcgtgtta ttgacgggcg cggtgagcct gttttccgac ttcacccatg aaggcgcgcg
     1441 cagcctgctt ggaccctatc tggcgctgct gggggccagc gcctttatcg tcgggctggt
     1501 gacgggcttc ggggagtttc tcggctacgc gctgcggctg gtttccggcc ggtgggcgga
     1561 cgcgaccggc agattctggc cgatcacgat cctgggctat gtcgtgcaga tggcggccgt
     1621 tccggccctg gccctgacgc atacctggcc gcaggccgcc gccctgatcg tgctggagcg
     1681 ggtcgggcgc gccatccgcg tgccgccgcg cgacgtcatg ctgtcgcacg ccgcgcacca
     1741 tatcggcggc tacggctgga ccttcggcat ccatcaggcg tgcgaccaga ccggcgcgct
     1801 gctggggccg ttgctgctgg ccctggtgat gggctggcgg ggcgattatc attttgcgtt
     1861 cgccgtcctg ctggttcccg ccacgatcaa tatcgccctg ctgctgatct cgcgccggct
     1921 gtatcccaac ccggaagcga tggaggtcca tccgccgcga accgcgcccg gcgaccggtt
     1981 ttcgcccgcc ttctgggtct atatggcggg cgcgtcgctg gtcgcgctgg gctttgccga
     2041 ttatccgctg atcgcctatc atttcgtgca tcataagatc gcgcccgtcg gctggatcgc
     2101 cggattctac gccgtggcga tggcggtgag cggcggcggc tcgctggtct tcgggcggct
     2161 ttacgaccgt ttcggcttca ggatgctggt cgtcctgacg atcgttgcgg cgctgttcgc
     2221 gccgctggcc ttcttcggtg atttctggct ggccctgatc ggcgccgcac tctgggggct
     2281 gggcatgggg gtgcatgaat cgatcattcc cgccgccgtg acgccgctcg ttccgccgtc
     2341 gaagcgcgcg tcggccttcg gcctgttcac cgccgtctat ggcgccttct ggttcgccgg
     2401 cagcgccgtc atcggttttc tttacgaccg gacgttgagc ggaaccgtta tcttctgcgt
     2461 cgcgacgcag atgctggcgg cgccggtctt catgtggctg gccggtcgcc ggtcaccgta
     2521 ggccgttggc ggcgggaaaa cgacgatccc aacgcctttc ttattttttg gcggcgaatt
     2581 ccggtaaatc ccggttgacg aaaagcgcgg cggtgctaac ctgatcctca gggaatggag
     2641 tttccctttt aaccgccttt tcggctgatg actcctgtac gcgcaaccgg cgctacagga
     2701 gtatttttat gtctccttca gcgcctttga ccggtcttcg gaccggaacc gaagggagac
     2761 gccccatgac gggtcaggtc gaatatctca ctcaggacgc catcggcgag ccgcacggct
     2821 catggcgcgg catgccggaa attccccgcc tcaagcggtc ctatacgacc ttgctgcagg
     2881 ccctgagcca cgcgcattac ggcgggctga agatgacgct gccggacggc gcgctgctgc
     2941 attaccacgg cgccgagcgc ggcgagacgg cccatatcca tattctcgac tggaaggcgt
     3001 ttgatcagat catcgc
//
LOCUS       DASVLL010000611         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_152865,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000611 DASVLL010000000
VERSION     DASVLL010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152865"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VF402_10030"
     CDS             complement(<1..652)
                     /locus_tag="VF402_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEX7800678.1"
                     /translation="MAQIFHTREILTADPARVVLLPFHIAISSRESAASVIARMKRIY
                     EAVAAMNADAVRTATARLERNFDARHRDLQDTLLSRFRQIRDAGGVGAALDDEKSALI
                     GAYFSHEYSFQAAALLNPSVVAHPDQSGLPGGATRFILSTRCVGEGHLSTIAFREGVF
                     HADGAIRLAPAAATASPAHMADDDVHEGPITLRKAPGDLSASVIFPMTRAQSNGLED"
     gene            871..1977
                     /locus_tag="VF402_10035"
     CDS             871..1977
                     /locus_tag="VF402_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7800679.1"
                     /translation="MWRRGLPPSPKSAGKVTIHDVARHAGVSSMTVSRVINKNVKVSD
                     ELTEKVLASVKALRYQLNTAASSTRSGQGRIRIGVLYSNPSAAFLNEILVGGFDQAAR
                     LGCQLMLEKCSGLHSQKTAVRRLLDEGVDGLILPPPLCDDRPRVQSLLREGLPLLALG
                     GGEPLPEVLSVRIDDFQGALTMTRYLIRLGHRRIGFIRGDEEHAPSQKRYEGFLQGMR
                     EAGLEIDEALIVRGRFTYQSGLAAAETLLQTPRRPTAIFASNDDMAAAVLAVAHGMRI
                     YVPDELSVCGFDDTPVASTVWPQITTIHQPIAAMGRAAVTAMTEAIKAARAGRDHGPA
                     HMTMKFSLIERGSAGPAPQLQIFRSQTEGGVLPR"
     gene            complement(2005..>2521)
                     /locus_tag="VF402_10040"
     CDS             complement(2005..>2521)
                     /locus_tag="VF402_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7800680.1"
                     /translation="GLGGLDILVNNAGLIRRQDAVDFSEKDWDDVMNVNIKSAFFMSQ
                     AAGRHFIAQGSGKIINIASMLSFQGGIRVPSYTASKSGIAGITRLLANEWASKGVNIN
                     AIAPGYMATDNTAQIRADEQREKAILDRIPAGRWGTPADLAGTAVFLAAQASDYVNGA
                     VIPVDGGWLAR"
BASE COUNT          406 a          762 c          925 g          428 t
ORIGIN      
        1 ggtcttcgag gccgttgctc tgcgcgcgcg tcatcgggaa gatcacgctg gccgacaggt
       61 cgccgggggc cttgcgcagg gttatcgggc cttcgtggac atcgtcgtcg gccatgtgcg
      121 ccgggctggc cgtggcggcg gcgggcgcca gccggatcgc gccgtcggcg tgaaagacgc
      181 cttcgcggaa ggcgatggtg gacagatggc cttcgccgac gcagcgggtg gacaggatga
      241 agcgcgtcgc gccgccgggc aggccggact gatcggggtg ggcgacgacc gaagggttga
      301 gcagggcggc ggcctgaaaa ctgtattcgt gcgagaaata ggcgccgatc agcgcgcttt
      361 tttcatcgtc gagcgcggcg ccgacgccgc ccgcgtcgcg aatctggcgg aagcggctca
      421 gaagcgtgtc ttgcaggtcg cgatggcggg cgtcgaaatt gcgctccagc cgcgccgtcg
      481 ccgtgcgcac ggcgtcggcg ttcatcgcgg cgacggcctc gtagatgcgc ttcatgcgcg
      541 cgatgacgct ggcggccgat tcgcgcgaac tgatggcgat gtgaaacggc agaagcacca
      601 cgcgggccgg gtcggccgtc aggatttccc gcgtatgaaa gatctgggcc aaggtgtgac
      661 gcctctgaac gatggagttt cgcggcccat tggtcgttct attcggaaga aatttcaagt
      721 gataaggtga ggcgccgccc cggcgcgcct tgtgacgagg tcttgtgcgg catggcggaa
      781 tgctgcaaat aatgaccgaa cgcctcgaat cctgcggcgc ccctcacggc gcccctcacg
      841 acgccgtaaa ggcgcgggtc cgggcgccga atgtggagaa gaggcttgcc gccatcacct
      901 aagtccgcgg gcaaagtgac cattcacgac gtggcgcgtc acgccggcgt ctcatccatg
      961 acggtgtccc gcgtcatcaa caagaacgtc aaggtcagcg acgaactgac cgagaaggtg
     1021 ctggcgtcgg tcaaggcgct caggtaccag ctcaatacgg cggcgtcctc gacgcgctcg
     1081 ggacagggac gcatccgcat cggcgtgctg tactccaacc cgtcggcggc ctttctgaac
     1141 gagattctcg tcggcggctt cgatcaggcg gcgcggctgg ggtgccagct catgctggag
     1201 aagtgttcgg ggctgcattc gcagaaaacc gccgtgcggc ggctgctgga cgagggggtg
     1261 gacgggctga ttctgccgcc gccgctgtgc gacgaccggc cgcgcgtgca gagtctgctg
     1321 cgcgaggggc tgccgttgct ggcgctgggc gggggcgagc cgctgccgga ggtgttgtcg
     1381 gtgcggatcg acgattttca gggcgccctg accatgacgc gctatctgat ccggctgggg
     1441 cacaggcgca tcggctttat ccgcggcgac gaggagcatg cgcccagcca gaagcgttat
     1501 gaaggctttt tgcagggtat gcgcgaggcc ggactggaga tcgacgaggc gcttatcgtc
     1561 cggggccggt tcacctatca gtccgggctg gccgccgccg agacgctgtt gcagaccccg
     1621 cgccgcccga ccgcgatctt cgcctcgaac gacgatatgg cggcggccgt gctggccgtc
     1681 gcgcacggga tgcgcatcta tgtgccggac gaactgagcg tgtgcggctt cgacgatacg
     1741 ccggtggcct cgaccgtgtg gccgcagatc acgaccattc accagccgat cgccgcgatg
     1801 ggcagggccg ccgtcaccgc catgacggag gcgatcaagg ccgcgcgcgc cggacgcgat
     1861 cacggaccgg cgcacatgac gatgaagttt tccctgatcg agcgcggctc ggcgggtccg
     1921 gcgccgcagc ttcagatttt ccgcagccag accgagggcg gcgttctgcc gcgatagaag
     1981 cgacaaaaca aaaacctaga gcgcttacct cgccagccac ccgccatcga cggggatgac
     2041 ggcgccgttg acgtagtcgg acgcctgtgc ggcgaggaag acggcggttc cggcgaggtc
     2101 ggcgggggtt ccccatcggc ctgcggggat gcggtcgagg atggcctttt cgcgctgttc
     2161 gtcggcgcgg atctgggcgg tgttgtcggt ggccatgtag ccgggggcga tggcgttgat
     2221 gttgacgccc ttgctggccc actcgttggc cagcaggcgg gtgatgcccg cgatgccgga
     2281 cttggaggcg gtgtaggagg gcacgcggat gccgccctgg aagctcagca tcgaggcgat
     2341 gttgatgatc ttgcccgaac cctgcgcgat gaagtgacgg cccgcggcct gcgacatgaa
     2401 gaaggcggac ttgatgttga cgttcatcac gtcgtcccag tccttttcgg agaagtcgac
     2461 cgcgtcctga cggcggatca gcccggcgtt gttgacgagg atgtcgaggc cgccgaggcc
     2521 g
//
LOCUS       DASVLL010000612         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_153097,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000612 DASVLL010000000
VERSION     DASVLL010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153097"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VF402_10045"
     CDS             complement(<1..941)
                     /locus_tag="VF402_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7800681.1"
                     /translation="MLRSAFLAFFRSFTRHPLYALLNLLGLSFGVAVFITLSLFYRFE
                     TSYENWTPQRSHIYVIGTGVHVPGIPDDLLLLTMGSLLDEIRSAYPQIDGVRDWPFPV
                     TMHRGAQIFSEQAESVDANFLSFFQAPVLRGSASSALSDPSQVVLSAHIARKYFGTID
                     VVGRPLTLGDESGLKTYTVSAVIADLPKNSDMQLDILRLLTPQHEAIMGGIWNGWGLI
                     LLKTYVKFKTPAEAAAFSAQLPAFIDRRAGATFGANVVPHKMLELPLTPLANSHLIRP
                     KLKAAILSLGLVGIVTLALALINYVNLATARAGMRAR"
     gene            complement(941..1645)
                     /locus_tag="VF402_10050"
     CDS             complement(941..1645)
                     /locus_tag="VF402_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7800682.1"
                     /translation="MTDPLIDLRNITRLYRSDEIETAALHDVSMSVKRGEFVAVMGPS
                     GCGKSTLLNIMGTIDRPTSGAYVFHGPDGQQDLARLSEVKLAQHRARHLGFIFQSFNL
                     VDDLTIFENIELGLLYRSHRHGSRRKAVEAAMDRVGIAHRARHYPHQLSGGQQQRAAI
                     ARAIVGEPDLILADEPTGNLDTENGAQVMDILRGLNAAGATIVMVTHSPSHADQASRR
                     IDMLDGQLAASVEQVL"
BASE COUNT          320 a          501 c          578 g          293 t
ORIGIN      
        1 tcgcgcgccc gcatgcccgc ccgcgccgta gccagattga catagttgat cagggccagc
       61 gccagcgtca cgatgccgac cagacccagc gacaggatcg ccgccttcag cttgggacgg
      121 atcagatggc tgttcgccag cggcgtaagc ggcagttcga gcatcttgtg cgggacgaca
      181 ttggcgccga aggtcgcgcc cgcccgacgg tcaatgaagg ccggaagttg ggcggaaaag
      241 gccgccgcct cggccggggt tttgaacttc acataggtct tcagcagtat caggccccag
      301 ccgttccaga ttccccccat gatcgcttca tgctgcggcg tcagcaggcg cagtatgtcg
      361 agttgcatgt cgctgttttt cggcaggtcg gcgatgacgg cgctgaccgt gtaggtcttg
      421 aggccgctct cgtcgccgag cgtcagaggc cggccgacga cgtcgatcgt gccgaaatat
      481 ttgcgggcga tatgggcgct cagcaccacc tgagacggat cggacagggc gctggaggcc
      541 gagcctcgca aaaccggcgc ctgaaagaag ctcaggaaat tggcgtcgac cgattccgcc
      601 tgctcgctga atatctgtgc gccgcgatgc atggtgacgg gaaagggcca gtcgcggacg
      661 ccgtcgatct gcggataggc cgacctgatc tcgtccagca ggctgcccat ggtcagcaag
      721 aggagatcgt cgggtatgcc gggcacatgg acgcccgtgc ctatgacata gatatgcgaa
      781 cgttgcgggg tccagttctc gtagctggtc tcgaaacgat agaacaggct cagcgtgatg
      841 aaaaccgcga cgccgaagga caggccgagc agattgagca gcgcatacag ggggtggcgc
      901 gtgaaagagc ggaaaaaggc gaggaaggcg gaacgcagca tcacagcacc tgttcgaccg
      961 aggccgccaa ctggccgtcc agcatgtcga tgcggcgcga ggcctgatcg gcgtggctgg
     1021 gcgaatgggt gaccatgacg atggtggccc ccgccgcatt cagtccgcgc agaatgtcca
     1081 tgacctgcgc gccgttctcg gtgtcgagat tgccggtcgg ttcgtcggcc aggatcaggt
     1141 cgggttcgcc gacaatggcg cgggcgatgg cggcgcgctg ctgctggccg cccgatagct
     1201 ggtgcggata gtgccgcgcc cggtgggcga tgccgacgcg atccatcgcc gcctcgaccg
     1261 ccttgcggcg cgaaccgtgc cggtggctgc ggtataacag gcccagttcg atattctcga
     1321 agatggtcag gtcatcgacg aggttgaagc tctggaagat gaagcccaga tggcgggcgc
     1381 ggtgctgcgc cagcttcacc tcgctcaggc gcgccagatc ctgctggccg tccggcccgt
     1441 gaaaaacata agcgccggag gtcgggcgat cgatcgtgcc cataatattg agcagggtcg
     1501 atttgccgca tcccgaaggc cccatgacgg ccacgaactc gccgcgtttc accgacatcg
     1561 aaacgtcgtg cagcgccgcc gtctcgatct cgtcggaacg gtaaagacgg gtgatgttgc
     1621 gcaaatcgat cagggggtcg gtcatgaggc gtcactcttg ctgaaacgga tgtgccgggc
     1681 gttggcgtag cc
//
LOCUS       DASVLL010000613         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_527824,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000613 DASVLL010000000
VERSION     DASVLL010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527824"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VF402_10055"
     CDS             <1..1545
                     /locus_tag="VF402_10055"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7800683.1"
                     /translation="RQDEQLIALDGKTYDIHPDMCVIADADGARPIGLGGVMGGISTG
                     VDETTTEVFLESAWFDPIRTAQTGRVTGIASDAQYRFARGVDPESTLPGLELATQLIL
                     SLCGGEASEVAGAGEALARKPDVAFDPAYVTKLTGMEIGADTTRAILTALGFTVSGDW
                     TVAPPSWRRDVEGKADLVEEVARIHGFDNLPATPLPVVPPQAGGVLTARQAKARIARR
                     ALAAYGYSEAVTWSFMRQDRAALFGGGGDRLVLANPIASELNCMRPSALPNLLEACGR
                     NAARGFTGAQLFEIGPVYAGLEPNDQKTVIAAVRAPDRTRGWQGQPEDALFALKGDLM
                     TVLEDMGAPVASLQLVQGANNGWWHPGRSARLQLGPRTVVAEFGELHPAALKAMDLDG
                     AYVGFEIVLENLPAGKAKSGKSKGALNLSNLMPLSRDFAFLIDARTPSGDLIRAIRGA
                     DKALIADARVFDVYTGAGVPEGQVSLAVEVTIAPTDKTLTEPEIEALRDRIVAAAQKA
                     GATLRG"
     gene            1603..1851
                     /locus_tag="VF402_10060"
     CDS             1603..1851
                     /locus_tag="VF402_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX7800684.1"
                     /translation="MDALSIGSASAVSAIRSFDQAAAQTVRDAAPQNAPSPNGIGGNG
                     DLAGDIVQQIQAQAAFQASVSVIRTADQMTGRVLDVKA"
BASE COUNT          322 a          681 c          705 g          300 t
ORIGIN      
        1 aggcaggacg agcaactgat cgcgctggac ggcaagacct acgacatcca tcccgacatg
       61 tgcgtgatcg ccgacgccga cggcgcgcgc cccatcggtc tgggcggcgt gatgggcggg
      121 atcagcaccg gcgtcgatga aaccaccacc gaggtcttcc tcgaatcggc gtggttcgac
      181 ccgatccgca ccgcgcagac gggccgcgtc acgggcatcg cctcggacgc gcaataccgc
      241 ttcgcgcgcg gcgtcgatcc cgaatcgacc ctgcccggcc tcgaactggc cacgcaactg
      301 atcctgagcc tgtgcggcgg cgaagcgtcc gaggtcgcgg gcgcgggcga ggctctggcg
      361 cgcaagccgg acgtggcctt cgacccggcc tatgtcacga agctgaccgg catggagatc
      421 ggcgccgata cgacgcgcgc catcctgacc gcgctgggct tcaccgtctc cggcgactgg
      481 accgtcgcgc cgccgtcgtg gcgccgcgac gtcgagggca aggccgatct ggtcgaggag
      541 gtcgcgcgca tccacggctt cgacaacctg cccgccacgc cgctgccggt cgttcccccg
      601 caagcgggcg gcgtgctgac cgcgcgtcag gccaaggcgc gcatcgcgcg ccgggcgctg
      661 gcggcctatg gttattccga ggcggtgaca tggtccttca tgcgtcagga ccgggcggcg
      721 ctgttcggcg gcggcgggga caggctggtg ctggccaatc cgatcgcctc ggaactgaac
      781 tgcatgcggc cctcggccct gcccaacctg ctcgaagcgt gcgggcgcaa cgcggcgcgc
      841 ggctttacgg gcgcgcaact gttcgagatc gggccggtct atgccggtct ggagccgaac
      901 gaccagaaga ccgtcatcgc cgccgtgcgc gcgccggacc gcacgcgcgg ctggcagggc
      961 cagccggagg acgcgctgtt cgcgctcaag ggcgacctga tgaccgtgct ggaggacatg
     1021 ggcgcgccgg tcgcctcgct gcaactggtt cagggcgcca ataacggctg gtggcatccg
     1081 ggccgttcgg cgcggctgca actcggcccc aggacggtgg tcgcggaatt cggcgaactg
     1141 catccggcgg cgttgaaggc gatggacctc gacggggcct atgtcggctt cgagatcgtg
     1201 ctggagaacc tgcccgcggg caaggcgaag tccggcaagt cgaagggcgc gctcaaccta
     1261 tccaacctga tgccgctgtc gcgcgacttc gccttcctga tcgacgccag gacgccttcg
     1321 ggcgacctga tccgcgcgat ccggggcgcg gacaaggcgc tgatcgccga tgcgcgcgtg
     1381 ttcgacgtct ataccggcgc gggcgtgccc gaaggccagg tgtcgctggc cgtcgaggtc
     1441 acgatcgcgc cgacggacaa gaccctgacc gaaccggaga tcgaggcgct gagggacagg
     1501 atcgtcgcgg cggcgcaaaa ggccggggcg accctgcgcg gttaacacag cttaaccgcg
     1561 tctttacaga ttcccttagg aaagcgggcg cataatgctg ttatggacgc tctctcgata
     1621 ggttccgcca gcgccgtttc ggccatccgc agcttcgatc aggccgcggc gcagacggtg
     1681 cgcgacgccg cgccgcaaaa cgcgccgtcg cccaacggca tcggcggcaa cggcgatctg
     1741 gccggcgata tcgtccagca gattcaggcg caggccgcct ttcaggccag cgtcagcgtg
     1801 atccggaccg ccgatcagat gaccggccgt gttctcgatg tgaaggcgta acctgtttcc
     1861 tcccctgccc caactggagc gccgagcgat cggcgctctt gttttatgtg gcggcgcgta
     1921 acgacgcggc aggatggcat ccccctctcc ccatgcacat ggggagagga cgaggagcaa
     1981 ggcgaagccg ccgatcctcg ggtgaggg
//
LOCUS       DASVLL010000614         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_418341,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000614 DASVLL010000000
VERSION     DASVLL010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418341"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VF402_10065"
     CDS             complement(<1..1330)
                     /locus_tag="VF402_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7800685.1"
                     /translation="MRTEPIAFGLITLAALTPLAAQAQTESGAAASSASSSSAAPAPQ
                     PAAKTPQTGQTQTGQTQAGQTQAGQTQEGPTVVTVTAQKPLIQHKIDRDVYDVTQDPQ
                     AATGAASDVLNNVPGVTVDNDGTVSLRGNSGVQVYVNGKKSAQMQGDERAFTLQSLPA
                     DDIDSIEVIPNPGAAYGADTAGGIINIVMKRGRSLKPHTMINVTAGTQGREGVNFRTG
                     KNIGKLHLNGGVNIGGGMGGGGRGGGGGGSTGGTRKSVSDSDTYTLDPLTGAVVRENV
                     QHRVTRSKNESLSANGSAEYDIDDNSDLTADLSYSRRHSTSTGAMQTKIYDPAHDLVS
                     DVTRLSNSSAPVENMDARLTYDHRGQVGSTEDFKMALSHSSNLSQGVTYTRNIDNTRA
                     TADTFTTQARKSKTSIDEFSGDWSHPLGDYDKTNQQIQLGWDVQHNVSDSY"
     gene            complement(1466..>2641)
                     /locus_tag="VF402_10070"
     CDS             complement(1466..>2641)
                     /locus_tag="VF402_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEX7800686.1"
                     /translation="EAQALENAAIMAQKLLPSGYDVFTIDIQWYEPAANGYAYNAHPE
                     PAMDGYGRLLPAPNRFPSAAGGQGFKPLADKIHALGLKFGIHLMRGIPRLAVDRNLPV
                     YGAQVRARDIADTDSTCPWNPDMYGVDMTKPGAQAYYDSVFALYAAWGVDFVKMDDMS
                     RPYDAHAPEIEAAHKAIQAAGRPIILSLSPGETPVARAGHVRQYAQMWRISDDFWDEW
                     PLLKAQFTRLENWNAHRSPGAWPDADMLPLGRLRMGKDDTKFTPDEQRTLMTLWAMAR
                     SPLIMGGDLRHLDDATLALLTQPDILAIHQQGRENQPWFLQDDARIWSARVGDRTYLA
                     LFNTADKPLTIGFDPARLGLSGGVRARDVWADADIAGAQGRIAPTVAPHGVKLLRLT"
BASE COUNT          386 a          827 c          887 g          541 t
ORIGIN      
        1 tatagctgtc gctgacattg tgctgcacgt cccagcccag ttgaatctgc tggttcgtct
       61 tgtcgtagtc gccgagcgga tgcgaccagt cgccgctgaa ttcgtcgatg gaggtcttcg
      121 acttgcgcgc ctgcgtcgtg aaggtgtccg ccgtcgcgcg ggtattgtcg atattgcgcg
      181 tataggtcac gccctggctc agattggagc tgtgcgacag cgccatcttg aagtcctcgg
      241 tcgagccgac ctggccgcga tggtcgtagg tcaggcgcgc gtccatattc tcgaccggcg
      301 cggacgaatt ggacaggcgc gtgacatcgc tgaccagatc gtgcgcgggg tcgtagatct
      361 tagtctgcat ggcgccggtc gaggtgctgt gacggcggga atagctgaga tcggcggtca
      421 gatcggaatt gtcgtcgata tcgtattcgg cgctgccgtt ggccgagagg ctctcgttct
      481 tcgagcgcgt gacgcggtgc tgcacgtttt cgcgcacgac ggcgccggtg agcggatcga
      541 gcgtataggt gtcgctgtcg ctgaccgact tgcgcgtgcc gccggtcgag ccgccgccgc
      601 cgccgccgcg tccgccgccg cccatgccgc cgccgatatt gacgccgccg ttcaggtgca
      661 gcttgccgat attcttgccg gtgcggaagt tgacgccttc gcggccttgc gttcccgccg
      721 tgacgttgat catggtgtgc ggtttgagcg agcggccgcg cttcatgacg atgttgatga
      781 tgccgcccgc cgtgtcggcg ccgtaggccg cgcccggatt ggggatgacc tcgatggaat
      841 cgatgtcgtc ggcgggcagg ctttgcagcg tgaaggcgcg ctcgtcgccc tgcatctggg
      901 ccgatttctt gccgttgaca tagacctgca cgccggaatt gccgcgtaag gagaccgtgc
      961 cgtcattgtc gaccgtgacg cccggcacgt tgttcagcac gtccgaggcg gcgccggtgg
     1021 cggcctgcgg gtcttgcgtc acgtcataga cgtcgcggtc gatcttgtgc tggatcagcg
     1081 gcttttgcgc cgtcaccgtc accacggtgg ggccttcctg agtctggcct gcctgagttt
     1141 ggcctgcctg agtctgaccg gtctgcgtct gaccggtttg aggcgttttg gcggcgggct
     1201 gaggcgccgg ggccgcggac gacgacgacg cgctggaggc ggcggcgccg gactccgttt
     1261 gcgcctgcgc cgccagaggc gtcagcgcgg ccagtgtgat cagaccgaaa gcaatgggtt
     1321 cagttcgcat gtaagtgccg gatatccgtg ttttttgttg tgccgcattg aacgcacaca
     1381 aaccacagtc tccgtctctt gaaatatcga acttgcaaaa aaatgatgtt tcctgattga
     1441 aatggtttat ttgccacatc ggccctcagg tcaggcgcag cagcttgacg ccgtgcggcg
     1501 ccacggtcgg cgctatgcgg ccttgcgctc cggctatatc cgcatccgcc cagacatcgc
     1561 gcgcccgcac gccgccgctc agccccagac gggccggatc gaagccgatc gtcagcggct
     1621 tatcggcggt gttgaacagg gccagatagg tccggtcgcc gacgcgcgcg ctccagatgc
     1681 gggcgtcgtc ctgcaagaac cacggctggt tttcgcggcc ctgctgatgg atggccagga
     1741 tgtcgggctg agtcagcagg gccagggtcg cgtcgtcgag atggcgcagg tcgccgccca
     1801 tgatcagcgg cgagcgcgcc atcgcccaca gcgtcatcag cgttctttgc tcgtccggcg
     1861 tgaacttcgt gtcgtccttg cccatgcgca gccgccccag cggcagcatg tcggcgtccg
     1921 gccacgcgcc ggggctgcga tgcgcgttcc agttttccag ccgcgtgaac tgggccttca
     1981 acagcggcca ttcgtcccag aaatcgtcgc tgatgcgcca catctgggcg tattgccgga
     2041 cgtgaccggc gcgcgccacc ggcgtttcgc cgggcgacag gctgaggatg atgggacgtc
     2101 ccgccgcctg aatcgccttg tgcgcggcct cgatctccgg cgcgtgcgcg tcgtagggac
     2161 ggctcatatc gtccatcttg acgaagtcga cgccccatgc ggcgtacagc gcgaacacgc
     2221 tgtcgtaata ggcctgcgcg cccggcttcg tcatgtcgac gccgtacatg tccgggttcc
     2281 acggacaggt gctgtccgta tcggcgatgt cgcgcgcgcg gacctgcgcg ccgtaaaccg
     2341 gcaggttcct gtccaccgcc agacgcggga ttccgcgcat caggtggatg ccgaacttca
     2401 agcccaaagc gtgaattttg tcggccagtg gtttgaaacc ttggcctccc gccgccgagg
     2461 gaaagcggtt cggcgcgggc agcaaccgcc cgtagccgtc catcgccggt tcgggatgcg
     2521 cgttgtaggc atagccgttc gccgccggtt cgtaccactg gatatcgatg gtgaagacgt
     2581 catagccgga cggcagcagc ttttgcgcca tgatggcggc gttttccagc gcctgcgcct
     2641 c
//
LOCUS       DASVLL010000615         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_312373,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000615 DASVLL010000000
VERSION     DASVLL010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312373"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VF402_10075"
     CDS             complement(<1..464)
                     /locus_tag="VF402_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7800687.1"
                     /translation="MSTSSQRPAGTTPFQGFDRQAPSRQIMGDAGSAEALRVLSKANE
                     HIRRRETLTLLNDALKRFGKGDWAGGGELALKALHIDEASAEAWHILGVARDKCHDVA
                     SAIACYEASLRLSPENVAVACDLGRLAFRIGETGMAEKFFRFHLSKCPDSVEA"
     gene            678..1073
                     /gene="flbT"
                     /locus_tag="VF402_10080"
     CDS             678..1073
                     /gene="flbT"
                     /locus_tag="VF402_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEX7800688.1"
                     /translation="MPLKLSLKPGEKFVLNGAVVQNGDRRCSLVLQNKASVLREKDIM
                     QVEDVNSPARHVYFPVMMMYLDEAGADKYYDEFLHRMTEFMGAISNPQVLAECVGVSR
                     SILAREFYKALMGCRKLIEYEDERLGHVA"
     gene            1063..1467
                     /gene="flaF"
                     /locus_tag="VF402_10085"
     CDS             1063..1467
                     /gene="flaF"
                     /locus_tag="VF402_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEX7800689.1"
                     /translation="MSLKAYQTTAARAEDPRQTEYRLFGQVTRALMEAEKLERSQIRE
                     RMDALDWNRRMWSMLAADCAGDGNKLPAQIRANIISLSLWVNRHTSLVMRNKEEIGPL
                     IDINRTIMQGLLPQRQAAQGETPPVMARQSYG"
     gene            complement(1576..>1813)
                     /locus_tag="VF402_10090"
     CDS             complement(1576..>1813)
                     /locus_tag="VF402_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7800690.1"
                     /translation="IYKTLIMPVICWGICRALGGDSLAQGVALCCGAAPSAAAYVLAR
                     HMGGDAPLMAGVIALTTILSAAAMPLLLIAFHLG"
BASE COUNT          349 a          566 c          591 g          307 t
ORIGIN      
        1 gcctcgacgc tgtcggggca tttcgacagg tggaagcgga agaacttttc cgccatgccg
       61 gtttcgccga tgcggaaggc cagccgcccg aggtcgcagg ccacggcgac gttttcgggg
      121 gacagccgca ggctggcctc atagcaggcg atggccgagg cgacgtcatg gcatttgtcg
      181 cgcgcgacgc caagaatgtg ccaggcctcg gcggaggctt cgtcgatatg cagcgccttg
      241 agcgccagct cgccgccgcc cgcccagtcg cccttgccga accgcttgag cgcgtcgttc
      301 agcagggtga gcgtttcgcg ccgccggata tgctcgttcg ccttgctcag gacgcgcaaa
      361 gcctcggcgg aaccggcgtc gcccatgatc tgacgcgagg gcgcctggcg gtcgaaaccc
      421 tgaaacggcg tggtcccggc ggggcgttgt gacgatgtgg acatggacta gctccgcgcg
      481 gcgtgtcgtg tcatcctgta aacagggatt cgcttaagac aacctaaaga atcgctggcc
      541 gggttgtcgc cgggccggtc ccgtcatcag tccccgcggc ggtcgtcccg acggcgtgcg
      601 aaaccggatg ttaacctcgg ttgcgctatt gggatcagga tcgtctgttc cgccccggcc
      661 ggcgcttagg aaataccatg ccgctcaaac tgtcgttgaa gcctggggaa aaattcgtcc
      721 tgaacggcgc cgtcgtgcag aacggcgacc gccgctgttc gctggtgttg cagaacaagg
      781 cgtcggtgct gcgcgaaaag gacatcatgc aggtcgagga cgtgaactcg cccgcgcgcc
      841 acgtctattt cccggtgatg atgatgtatc tggacgaggc gggcgccgac aaatactacg
      901 acgaattcct gcaccgcatg accgaattca tgggcgcgat ctccaatccg caggtgctgg
      961 ccgaatgcgt cggcgtctcc agaagcattt tggcgcgcga attctacaag gcgttgatgg
     1021 gctgccgaaa gctcatcgag tacgaggacg agagactggg acatgtcgct taaagcgtat
     1081 cagaccaccg ccgcccgcgc cgaggatccc cgccagaccg agtaccgtct gttcgggcag
     1141 gtcacgcggg cgttgatgga ggctgagaag ctggagcgtt cgcagattcg cgagcgcatg
     1201 gacgcgctgg actggaaccg gcgcatgtgg tcgatgctgg cggcggactg cgccggcgac
     1261 ggcaacaagc tgcccgcgca gatccgcgcc aacatcatct cgctgtcgct ctgggtgaac
     1321 cggcatacct cgctggtcat gcgcaacaag gaagagatcg ggccgctgat cgacatcaac
     1381 cgcaccatca tgcagggcct gttgccgcag cgtcaggcgg cgcagggcga gacgccgccc
     1441 gtcatggcgc ggcaatccta cggctagagc gctttccgaa aagtgtgacg cacttttcgg
     1501 attaaaaagc gcgtaaaagc ataaagttag agcgccgatc tgatgcaatc agatcggaaa
     1561 atgctctaaa tacgcttacc ccagatgaaa cgcgatgagc agcagcggca tggccgccgc
     1621 gctcaggatc gtggtcaggg cgatcacgcc cgccatcagc ggcgcgtccc cgcccatgtg
     1681 ccgggccagc acataggccg ccgccgacgg cgcggcgccg cagcacaggg ccacgccctg
     1741 cgccagcgaa tcgccgccca gcgcgcggca tatcccccag cagatcaccg gcatgatcag
     1801 cgtcttgtag atc
//
LOCUS       DASVLL010000616         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_364830,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000616 DASVLL010000000
VERSION     DASVLL010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364830"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VF402_10095"
     CDS             <1..412
                     /locus_tag="VF402_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800691.1"
                     /translation="DNAAAGANTGGRLGNVQLPGGLKGGRGAVSQALQNHDYCVDRQV
                     DGKPIPKDCDMPDLASAKPLGPKRVAAFDAELARRKMIAKPGNSDYWDRVVNPIQDPG
                     HDDHRARPGQYHTGKAARVLGECSQTDSCPTDGQ"
     gene            complement(416..1669)
                     /locus_tag="VF402_10100"
     CDS             complement(416..1669)
                     /locus_tag="VF402_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX7800692.1"
                     /translation="MGLLRSLRGWLDAFRRQSHGNAALVFALSLVAVVGAGGGALDVT
                     SAVNSQHDLQDAIDAAVLAGVAASSDSASVASQTFAENLDKRIAVTTQTYTASSASSS
                     SAGVVSVSTQLAGTAAGHAPTYMLQLFGISQINLSVNATAVSTRKQSPAGPCIYVLDP
                     SGSQALLVNSGAQVQAPNCEIDVASTGNPAAIFNSGSSLNFKTLCLQGTQTIQNSTTV
                     ANLKTGCATKADPYAGKLPTPSSSTCNYSNGNYNDATVNLSPGVYCGWFNFNNSSAKV
                     NFAPGVYVIKNGGWNVNGGTWTGSGVTFYFADTSKIQFNSGISATLSAPSGGTYANLL
                     FYEAAGLSKSDFIFNDSVANNLTGVIWLPSRNVTFNAKSGVASDSLTMIVWRLILNNT
                     TWSLGPLSGDSSSSGSGASTIRLIR"
     gene            complement(1769..2203)
                     /locus_tag="VF402_10105"
     CDS             complement(1769..2203)
                     /locus_tag="VF402_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body protein FlbY"
                     /protein_id="HEX7800693.1"
                     /translation="MALTANNGSERARQMLALTLRLAERLERETRSLQAHRPQDIAAE
                     VEETRSLSNLYRRESLRIKTDPSLLDGMTPEEKRQLREATVIFQTRLQAYEHAVNAAR
                     IVTEGILSAVARDLTKTRAATYGARGRAVDKGPQSFNFGGRA"
     gene            complement(2203..2526)
                     /locus_tag="VF402_10110"
     CDS             complement(2203..2526)
                     /locus_tag="VF402_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEX7800694.1"
                     /translation="MDTSALLTLQNAQTASGAAAQQTLATQMKSGKSNAKLGLDFESM
                     CLSNLLKPMFEGLSTDGPFGGGDGEAAMRSFFIDAIGKQMAQRGGIGISDMMQKQLLK
                     LQGAH"
     gene            complement(2529..3635)
                     /locus_tag="VF402_10115"
     CDS             complement(2529..3635)
                     /locus_tag="VF402_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX7800695.1"
                     /translation="MWKRMTALSAAALIGLAAHGHAQAASRIKDIVDVEGVRTNQLIG
                     YGIVVGLNGTGDTVRNSPMLKQSLEGMMERLGVNIRDADINTKNAAAVMVTAELPPFA
                     ASGSTLDVSVSAMGDAKSLLGGTLLVTPLIGADGEAYAAAQGTVQTGAVSATGASGSS
                     ITRGVPTAGRIAAGGTVERETNFDFNAMPVVRLTLRNPDFTTARRIADVIDRQYPGTA
                     LAENPTIVAIKPPAGTSMMDFVTRIESMDVDVDAPAKVVIDEVNGVIVVGENVRISRV
                     AIAQGNLTIKVDEQPQVSQPLPFSQGQTTTTPSSQVSVDEDKGKQMIELGGGSTLSDL
                     VAGLNALKVTPRDMIAILQSIKAAGALQADIEVM"
BASE COUNT          598 a         1167 c         1296 g          668 t
ORIGIN      
        1 ggacaatgcc gccgccggcg ccaataccgg gggccgtctc ggcaacgtgc aactgcccgg
       61 cgggctgaaa ggcgggcgcg gcgcggtcag tcaggccttg cagaaccacg attactgcgt
      121 cgaccggcag gtcgacggca agccgatccc caaggattgc gacatgcccg acctggccag
      181 cgccaagccg ctggggccga agcgcgtcgc ggcgttcgac gccgaactgg cgcggcgcaa
      241 gatgatcgcc aagccgggca acagcgatta ctgggaccgc gtcgtcaatc cgattcagga
      301 tcccggccat gacgaccacc gcgcgcgccc cggccagtat cacaccggca aggcggcgcg
      361 ggtgctgggc gaatgttcgc agaccgactc ctgcccgacg gacgggcagt agcggtcagc
      421 ggatcaggcg gatagtgctg gcgccggagc cggacgagga actgtcgccg ctgagcggcc
      481 ccagcgacca ggtcgtgttg ttcaggatca gccgccagac gatcatggtc agcgagtcgc
      541 tggcgacgcc cgacttggcg ttgaaggtca cgttgcggct gggcagccag atcacgccgg
      601 tcaggttgtt ggcgacggaa tcgttgaaga tgaagtcgct cttggacagg cccgccgcct
      661 cgtagaacaa gaggttggcg taggttccgc cgctgggcgc gctgagcgtg gccgagatgc
      721 cgctgttgaa ctggatcttc gaggtgtcgg cgaaatagaa ggtcacgccc gaaccggtcc
      781 atgtcccgcc gttgacgttc cagccgccgt tcttgatgac atagacgccg ggcgcgaaat
      841 tgaccttggc cgatgaattg ttgaaattga accagccgca atagacgccg gggctcagat
      901 tgaccgtggc gtcgttgtaa ttgccgttgg aatagttaca ggtcgacgag gacggcgtcg
      961 gcagcttgcc cgcatacgga tcggccttgg tcgcgcagcc ggtcttgaga ttggccaccg
     1021 tggtcgaatt ctggatggtc tgggttcctt gcaggcacag cgtcttgaaa ttcaggctgc
     1081 tgccggaatt gaagatggcc gccggattgc cggtcgaggc gacgtcgatc tcgcagttcg
     1141 gcgcctggac ctgtgcgccg ctgttgacca gcagggcctg cgagccggac gggtccagca
     1201 catagatgca cggtcccgcc gggctttgct tgcgcgtcga caccgccgtg gcgttgaccg
     1261 acaggtttat ctgcgagatg ccgaacagtt gcagcatgta ggtcggcgcg tgtccggcgg
     1321 cggtcccggc cagttgcgtg ctgacgctga cgacgcccgc cgacgagctg gacgccgacg
     1381 aggcggtgta ggtctgcgtc gtgaccgcga tgcgcttgtc gaggttttcg gcgaaggtct
     1441 ggctggcgac gctggcgctg tcggacgagg cggcgacccc ggccagcacc gccgcatcga
     1501 tggcgtcttg caagtcatgc tgcgaattca cagcggaggt cacgtccagc gcgccgccgc
     1561 cggcgccgac gaccgcgacc agggacaggg cgaacaccag agcggcgttg ccatgcgact
     1621 gacggcggaa ggcgtccagc catccgcgca aacttctcaa caggcccatt tttttatatc
     1681 tccccacagg ctggcgccat attaaccaga agggagtgaa gcccggctta aggcttatgg
     1741 tgaacgaagg cttgccgccc cggacccatc aggcgcgtcc gccgaagttg aaggattgcg
     1801 gccccttgtc gacggcgcgt ccgcgcgcgc cgtaggtcgc ggcgcgggtc ttggtcaggt
     1861 cgcgggcgac ggcgctcaat atgccttcgg tgacgatgcg cgcggcgttg accgcgtgtt
     1921 cgtaagcctg tagccgcgtc tggaaaatca ccgtcgcctc gcgcaactga cgcttttcct
     1981 cgggcgtcat gccgtccagc agcgacgggt cggtcttgat gcgcagcgat tcgcgccggt
     2041 acaggttgga caggctgcgc gtctcctcga cctcggcggc gatgtcctgc gggcgatgcg
     2101 cctgaaggct gcgcgtctcg cgctccagcc gctcggccag ccgcagggtc agggccagca
     2161 tctggcgcgc gcgctccgaa ccgttgttcg ccgtgagggc catcagtgcg ctccttgcag
     2221 cttgagaagc tgcttctgca tcatgtcgga aatgccgatg ccgccgcgct gcgccatctg
     2281 cttgccgatg gcgtcgataa agaaggagcg catggcggct tcgccgtcgc cgccgccgaa
     2341 cggaccgtcg gtcgacaggc cctcgaacat cggcttgagc aggttggaca ggcacatcga
     2401 ctcgaaatcg aggccgagct tggcgttgga tttgccggat ttcatctgcg tggccagggt
     2461 ttgctgcgcg gcggcgccgg aagcggtctg ggcgttttgc agggtgagaa gagcgctcgt
     2521 gtccatgatt acatcacctc gatatcggct tgcagcgcgc cggcggcctt gatggattgc
     2581 aggatggcga tcatgtcgcg cggcgtcacc ttcagcgcgt tcaggcccgc caccagatcg
     2641 gacagggtcg agccgccgcc cagctcgatc atctgcttgc ccttgtcctc gtcgacgctg
     2701 acctgactgg acggcgtggt ggtggtctgg ccctgcgaga agggcagggg ctggctgacc
     2761 tgcggctgct cgtcgacctt gatggtcagg ttgccctggg cgatggcgac gcgcgagatg
     2821 cgcacgtttt cgccgaccac gatcacgccg ttgacctcgt cgatcaccac cttggccggg
     2881 gcgtcgacat cgacgtccat cgactcgatg cgggtgacga agtccatcat gctggtcccg
     2941 gcgggcggct tgatggcgac gatggtgggg ttttcggcca gggccgtgcc cggatactgg
     3001 cggtcgatca cgtcggcgat gcggcgcgcg gtggtgaagt cgggattgcg cagggtcagg
     3061 cgcacgaccg gcatggcgtt gaagtcgaaa ttggtttcgc gctcgaccgt gccgcccgcg
     3121 gcgatgcggc ccgcggtcgg cacgccgcgc gtgatggacg agccggacgc gccggtggcc
     3181 gacaccgcgc cggtctgcac cgtgccctgc gccgccgcat aggcttcgcc gtcggcgccg
     3241 atcagcggcg tgaccagcag ggtgccgccc agaaggctct tggcgtcgcc catcgccgag
     3301 acgctgacgt cgagcgtcga gccggacgcc gcgaacggcg gcagctccgc cgtgaccatg
     3361 acggcggcgg cgttcttggt gttgatgtcg gcgtcgcgga tattgacgcc gaggcgctcc
     3421 atcatgcctt ccaggctctg cttgagcatg ggcgagttgc gcaccgtgtc gcccgtgccg
     3481 ttcaggccga cgacgatgcc gtagccgatc agctggttgg tgcgcacgcc ctcgacgtcg
     3541 acaatgtcct tgatgcgcga ggcggcctgc gcgtggccat gggccgccag accgatcagg
     3601 gccgccgcgc tgagcgccgt catccgtttc cacatcatcg tcatccgcct cgaaatgcgc
     3661 gcgttaacca tactgcgccc ttgtctgcga agaccgtgcc agagtctgtt gataaaataa
     3721 ttgagtaaa
//
LOCUS       DASVLL010000617         8646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_312724,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000617 DASVLL010000000
VERSION     DASVLL010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312724"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1522)
                     /gene="leuC"
                     /locus_tag="VF402_10120"
     CDS             complement(125..1522)
                     /gene="leuC"
                     /locus_tag="VF402_10120"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7800696.1"
                     /translation="MTAKSLFEKVWDAHVVEPEGAHSPAILYIDLHLVHEVTSPQAFS
                     ELESRGLKVRRPDRTFATLDHSIPTLAPDAAGNRPYVTAQAKKQVETLEANAANNGVR
                     LFGYDAENRGVVHVIGPEMGLTQPGFTVVCGDSHTSTHGAFGCIAFGIGTSEVGHVLA
                     TQCLAQRKPRTMRINIEGKLQPGVTGKDVALTVINTLGFSGGTGYALEYAGSVIRDLD
                     MEGRMTVCNLSIEAGARCGMIAPDEKTVEWLRGREYAPSGAAFDEAAKQWLSLKSDDG
                     AVFDKEITIDGSKIEPMATWGTSPDQGAPIVAGVPVAANEDQQRALKYMGFTGGEARE
                     VEKVDVVFIGSCTNGRMADLRAAAEIFKGRKVAPGVRALVVPGSEWVRIEAEKEGLHE
                     IFLAAGAEWRLPGCSMCIAMNGDFVQPGQLAVSTSNRNFEGRQGYKGRTHLVSPTMAA
                     AAAIAGRFVDVRTFR"
     gene            complement(1680..2624)
                     /locus_tag="VF402_10125"
     CDS             complement(1680..2624)
                     /locus_tag="VF402_10125"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX7800697.1"
                     /translation="MTKLADKIWINGETVAWDDARIHVMTHALHYGTSVFEGIRAYDT
                     PQGPVGFRLTDHLRRLEDSARIYGIKVPYTLAEMVQACKDILKANNLKGAYWRPIAFL
                     GENGMGVTPKGDNIKVDLAIAAFAWGAYLGEEGLNAGVDVCVSSWQRAAPNTIPNAAK
                     MGGNYLSSYLIGKEARGRGYVEGIGLGTDGRLSEGAGENLFLIMNGKIHTPPVASSIL
                     GGITRDSIIKLAKADGIEVIEQALPREMLYLADEVFMCGTAAEITPIRSVDDIPTRAG
                     GPGKVTKRLQTLFHGLFNGATEDRFGWLEPIEATVPAE"
     gene            complement(2781..3068)
                     /locus_tag="VF402_10130"
     CDS             complement(2781..3068)
                     /locus_tag="VF402_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7800698.1"
                     /translation="MSHLIHIELDRLEGSLVRVIGLIERRGFHIDALELYGLGEDRRG
                     ATVTVRPRDPSRNADMLGLQIDRLYGVRRLSEAPDADKARPQAAAKQKQGV"
     gene            complement(3046..4731)
                     /gene="ilvG"
                     /locus_tag="VF402_10135"
     CDS             complement(3046..4731)
                     /gene="ilvG"
                     /locus_tag="VF402_10135"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HEX7800699.1"
                     /translation="MSNQPTGAQLVISTLKALGVETLFGYPGGAIMPIYDALAGSGLQ
                     HILVRHEQGAAFAADAWARATGKVGVCMATSGPGATNLITGIANAYMDSVPMVCITGQ
                     VASHLMGTDGFQEVDILGITLPIVKHSWLVRDPADIPAILAEAFAIAAEGRPGPVLVD
                     IPKDVASAHVAASHPASFPAWTAPAPDPKAIEAATRLIEQAKKPLFYAGGGVEMAGAS
                     PAMLELVRRTGIPIVTTLKGIGVVPTNEELYLGMLGMHGTRAANTAVQETDLLIALGA
                     RFDDRATGKLNAFAPHAKVIHADIDAAEINKLRKADIAIHGDLKTTIESLRPKVSGIQ
                     KWVEQCKADKWEHRFRYNAPGQGVYAPALLKMLSEKAGDKFIAACDVGQHQMWVAQHC
                     EFPNCRAHLTSGGSGAMGYGLPAGVGAKLAKPDHHVVTISGDGSFMMNIQEMATLNRY
                     QIPLKIVLLDNSSLGLVRQWQQLFYQENYSEIDLSDNPDFVVVAKAFGLDAFRITHRH
                     EVEGAIDRLLAAEGPTLLHVLIDPRENVWPLVPPGASNAEMMEERWDEPLDSY"
     gene            complement(4906..5907)
                     /gene="ilvC"
                     /locus_tag="VF402_10140"
     CDS             complement(4906..5907)
                     /gene="ilvC"
                     /locus_tag="VF402_10140"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX7800700.1"
                     /translation="MKIYTNDDVSPDSLKGERIAIIGYGSQGRAHASNLKDSGADVVV
                     GLRPGGAGEVKAKADGHTVLSVADACKEATIIAILTPDMTHADLYKDVIEPNAPKGVA
                     LLFAHGFSVLYGRVVPRADMDVILVAPKGPGDLVRREYQRGRGVPSLFAVDRDVTGKA
                     AQKALGYARGIGGTVGGVIETSFKEETETDLFGEQAVLCGGTTELVMMGFETLVEAGY
                     RPEIACFECLHELKLIVDLLYEGGFAKMHSFVSETCKYGDLVSGPRIINEETRERMRA
                     VLKDIQDATFAREWILENQAGRPRYNSLMNADLNHQIEKVGADLRSRMSWLQNKQEA"
     gene            complement(5957..6529)
                     /locus_tag="VF402_10145"
     CDS             complement(5957..6529)
                     /locus_tag="VF402_10145"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7800701.1"
                     /translation="MNAIANPNRYNAAPYHGAGDVVRGAVGGFAILYGALAPEGCVAR
                     LDAFDEAVFDGPARVFDSGAEAVAGIAAGRVRSCDILIVRDDAAFDAGAVQSALDAAG
                     LERVVLITDAPLSDGAVIGQVAPSAAARGPIAYVNDDDIIHIDLSARRIDVMADIDLR
                     RSVRAQKPGRAVFGAGALQKYARLVTAAHT"
     gene            complement(6625..6774)
                     /locus_tag="VF402_10150"
     CDS             complement(6625..6774)
                     /locus_tag="VF402_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800702.1"
                     /translation="MSKVKVIAMKQDASQGFVALASSALFVLLVLDIVSLLSGGVLRP
                     FMPEW"
     gene            7127..>8646
                     /gene="ilvD"
                     /locus_tag="VF402_10155"
     CDS             7127..>8646
                     /gene="ilvD"
                     /locus_tag="VF402_10155"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7800703.1"
                     /translation="MPAYRSRTTTFGRNMAGARALWRATGMKDGDFTKPIVAIANSFT
                     QFVPGHVHLKDLGQLVAREIERAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADALVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKVVWPDESG
                     VAREHALDLIDAMMQAGDDRVSDAQVQAVEEAACPTCGSCSGMFTANSMNCLTEALGL
                     SLPGNGTTLATHSDRERLFCEAGRTIVDLAERWYEQDDASVLPRSIASKEAFENAMAL
                     DVAMGGSTNTVLHILAAAQEAGVDFTMADIDRISRNTPCICKVAPSVANVHIEDVHRA
                     GGIMGILGELNRGGLIHGEQPTVHAPSLAAALAHWDVKTSNDSSVSEFFKAAPGGVRT
                     TQAFSQSKRWSELDTDRQKGVIRSVEHAFTKDGGLAVLYGNIAEKGCIVKTAGVDESI
                     WKFTGKARVFESQDAAAAAILNNQIQAGDVVVIRYEGPKGGPGMQEMLYPTTYLKS"
BASE COUNT         1445 a         2802 c         2865 g         1534 t
ORIGIN      
        1 accttgttga cgttctggcc gccggcgccg gaggcgcgca ggaacgcgaa ctcgatctcg
       61 tcgtcgggga tcgcgatcca cgcgttcacg gcgaggggca tggcgcaggc ctgcccctcc
      121 gagcctacct gaacgtcctc acgtcgacga accgcccggc gatcgcggcg gccgccgcca
      181 tcgtcggcga gacgaggtgg gtgcggccct tgtagccttg gcggccctcg aaattgcggt
      241 tcgaggtcga gacggccagt tgccccggct gcacgaagtc gccgttcatg gcgatgcaca
      301 tcgaacagcc cggcaggcgc cattccgcgc ccgccgcgag gaagatttcg tgcaggccct
      361 ctttctcggc ctcgatccgc acccattccg aacccggcac gaccagggcg cgcacgcccg
      421 gcgccacctt gcggcccttg aaaatctcgg cggcggcgcg caggtcggcc atgcggccgt
      481 tggtgcagga gccgatgaac acgacgtcga ccttttcgac ctcgcgggct tcgccgcccg
      541 tgaagcccat atatttcagc gcgcgctgct gatcctcatt ggcggccacc ggcacgcccg
      601 caacgatcgg cgcgccctgg tcgggcgagg tgccccaggt cgccatcggc tcgatcttcg
      661 agccgtcgat ggtgatttcc ttatcgaaga ccgcgccgtc atccgatttc agcgacagcc
      721 actgcttcgc ggcttcgtcg aacgccgcgc cggacggcgc gtattcgcgc ccgcgcagcc
      781 attcgaccgt cttttcgtcc ggcgcgatca tgccgcagcg cgcgcccgcc tcgatcgaca
      841 ggttgcacac cgtcatgcgg ccttccatgt cgaggtcgcg gatcaccgaa ccggcgtatt
      901 ccagcgcata gccggtgccg ccggagaagc cgagcgtatt gatcacggtc agcgccacgt
      961 ccttacccgt cacgcccggt tgcagtttgc cctcgatatt gatgcgcatg gtcctgggct
     1021 tgcgctgcgc cagacattgc gtcgccagca cgtgcccgac ctcggaggtg ccgatgccga
     1081 aggcgatgca gccgaaggcg ccgtgggtcg aggtgtgcga atcgccgcag accacggtga
     1141 agcccggctg ggtcaggccc atttccggcc cgatgacgtg aacgacgccg cggttttcgg
     1201 cgtcatagcc gaacaggcgc acgccgttat tggccgcgtt ggcctccagc gtctcgacct
     1261 gctttttcgc ctgcgcggtc acgtaggggc ggttgcccgc cgcgtccggc gccagggtcg
     1321 ggatcgagtg atccagcgtg gcgaaggtgc ggtccgggcg gcgcaccttg aggccgcgcg
     1381 actccagctc ggagaaggcc tgcggcgagg tgacctcgtg gacgaggtgc aggtcgatgt
     1441 acaggatggc ggggctgtgc gcgccttcag gctcgacgac gtgggcgtcc cacacttttt
     1501 cgaataagga ttttgcggtc atgggtcttc gtctctggcg gttgactctt cgccctcccc
     1561 gtttacgggg aggggggacc atccgtagga tggtgggcgg ggcggcccct tccgtctttg
     1621 ggcgcaggcg cccaaagcca cctcccccgc aagcggggga ggccgaagag gtgagttact
     1681 tattcggccg gaaccgtcgc ctcgatcggc tcaagccagc cgaagcgatc ttccgtcgcg
     1741 ccgttgaaca gaccgtggaa cagggtctgc aaacgcttgg tgaccttgcc ggggccgccg
     1801 gcgcgggtcg ggatgtcgtc gaccgagcgg atcggcgtga tctcggcggc ggtgccgcac
     1861 atgaacactt cgtcggccag gtacagcatt tcgcgcggca gggcctgctc gatgacctcg
     1921 atgccgtcag ccttggctag cttgatgatc gagtcgcgcg tgatgccgcc gaggatggac
     1981 gaagcgaccg gcggggtgtg gatcttgccg ttcatgatca ggaacaggtt ttcacccgcc
     2041 ccttccgaca gacgcccgtc cgtgccgagg ccgatgccct cgacatagcc gcgcccgcgc
     2101 gcctccttgc cgatcaggta ggacgacagg tagttgccgc ccatcttggc cgcgttcgga
     2161 atggtgttcg gcgcggcgcg ctgccacgac gagacgcaga cgtcgacgcc cgcattcagg
     2221 ccctcttcgc ccagataggc gccccaggcg aaggcggcga tggccaggtc gaccttgata
     2281 ttgtcgccct tgggcgtgac gcccatgccg ttttcgccga ggaaggcgat gggccgccaa
     2341 taggcgcctt tcaggttgtt ggccttgagg atgtccttgc acgcctgaac catctcggcc
     2401 agcgtgtagg gaaccttgat gccgtagatg cgcgccgaat cttcgagacg gcgcagatgg
     2461 tcggtcaggc ggaagccgac cggcccctgc ggcgtgtcat aggcgcggat gccctcgaaa
     2521 acggaggtgc cgtaatgcag ggcgtgggtc atgacgtgga tcctggcgtc gtcccaggcg
     2581 acggtttcgc cgttgatcca gattttgtct gcaagcttgg tcatgtgaat tttgccattc
     2641 ggtagagagt ttggaaacgt ctgtaccccc tttctcgggg gtcagaaagc cggaatttca
     2701 ccttccattc cctaccggac cggggccgcg acaacggccc cgacgacctt ctccatagca
     2761 tcgatgcgaa tgcggcatgt tcagacgccc tgcttctgct tcgcggcggc ctgcgggcgc
     2821 gccttgtccg cgtccggggc ctcgctcagc cgccggacgc cgtacagccg gtcgatctgc
     2881 aaacccagca tgtcggcgtt gcgcgacgga tcgcgcgggc gcaccgtcac cgtggcgccg
     2941 cgccggtcct cgccgaggcc gtaaagctcc agcgcgtcga tgtggaagcc gcgccgctcg
     3001 atcagaccga tcacccggac aaggctgcct tcgagacgat ccagctcaat atgaatcaag
     3061 tggctcatcc cagcgctcct ccatcatttc cgcgttcgac gcgcccggcg gcaccagcgg
     3121 ccagacattt tctctggggt ctataagaac atgcaacagg gtcggaccct cggcggccag
     3181 cagccggtcg atggcgccct cgacttcgtg gcggtgggtg atgcggaagg catcgagccc
     3241 gaacgccttg gcgacgacga cgaagtcggg attgtccgac aggtcgatct cgctgtaatt
     3301 ttcctgatag aacagttgct gccactgacg gaccagaccc agcgaggaat tgtccagaag
     3361 cacgatcttc agcgggatct ggtaacggtt gagcgtcgcc atctcctgaa tgttcatcat
     3421 gaacgagccg tcgccggaaa tggtgacgac atggtggtcc ggcttggcca gcttggcgcc
     3481 gacgccggcg ggcaggccat agcccatcgc gcccgacccg cctgaggtca ggtgcgcgcg
     3541 gcagttgggg aattcgcagt gctgcgccac ccacatctga tgctggccga cgtcgcaggc
     3601 ggcgatgaac ttgtcgcccg ccttttccga cagcattttc agcagggcgg gcgcatagac
     3661 gccctgaccc ggcgcgttgt agcggaagcg atgctcccac ttgtcggcct tgcactgctc
     3721 gacccacttc tggatgccgg acaccttggg gcgcaggctc tcgatggtgg ttttcaggtc
     3781 gccgtgaatg gcgatgtcgg ccttgcgcag cttgttgatc tcggccgcgt cgatgtcggc
     3841 gtggatgacc ttggcgtgcg gcgcgaaggc gttgagcttg cccgtggcgc ggtcgtcgaa
     3901 gcgcgcgccc agcgcgatca gcaggtcggt ttcctgcacc gcggtattgg ccgcgcgcgt
     3961 gccgtgcatg cccagcatgc ccaggtacag ctcctcgttc gtcggcacga cgccgatgcc
     4021 cttgagcgtg gtgacgatgg ggatgccggt gcggcggacc agttccagca tggcgggcga
     4081 ggccccggcc atttcgacgc cgccgccggc gtagaacaac ggctttttgg cctgctcgat
     4141 cagcctggtc gcggcttcga tcgccttggg gtcgggcgcg ggcgcggtcc aggccgggaa
     4201 gctggccgga tgcgaggccg cgacgtgcgc cgaggcgacg tccttgggga tgtcgaccag
     4261 caccgggccg gggcggccct cggcggcgat ggcgaaggcc tcggccagaa tggccggaat
     4321 gtcggcgggg tcgcggacca gccacgaatg tttgacgatc ggcagggtga tgccgagaat
     4381 atcgacttcc tgaaagccgt ccgtgcccat caggtgcgag gcgacctgac cggtgatgca
     4441 gaccatcggc acggagtcca tataggcgtt ggcgatgccg gtgatcaggt tggtggcgcc
     4501 gggaccggag gtggccatgc agacgccgac cttgccggtg gcgcgcgccc aggcgtcggc
     4561 ggcgaaggcc gcgccctgct cgtggcggac cagaatatgt tgcaggccgg acccggccag
     4621 cgcgtcgtag atgggcatga tcgccccgcc cggatagccg aatagcgtct cgacccccaa
     4681 cgccttcagc gtggagatga ccagttgcgc tccggtgggt tggttgctca tcgtttcaac
     4741 tcgattgttt acgttaaact ccgccgccct ctcctccccg tcgtgatggg gagggggacc
     4801 gcgaagcggt gggaggggga cgccgacggt ggccttcccc cttccgtctt tgaaagccaa
     4861 cgctttcaaa tccaccttcc ccgtaaacgg ggaaggacga aggctttacg cttcctgctt
     4921 gttttgcagc cagctcatgc gcgaacgcag gtccgcgccg actttttcga tctggtggtt
     4981 caggtcggcg ttcatcagcg aattgtaacg cggacggccg gcctgatttt ccagaatcca
     5041 ttcgcgcgcg aaggtggcgt cctgaatgtc cttcagcacg gcgcgcatgc gctcgcgggt
     5101 ttcctcgttg atgatgcgcg ggcccgaaac cagatcgccg tacttgcagg tttccgacac
     5161 gaaggagtgc atcttggcga agccgccttc gtacaggagg tcgacgatca gcttcagttc
     5221 gtgcaggcac tcgaaacaag cgatttccgg acggtaaccg gcttcgacca gcgtctcgaa
     5281 gcccatcatg accagctcgg tcgtgccgcc gcacaggacg gcctgttcgc cgaacaggtc
     5341 ggtttcggtt tcttccttga acgaggtctc gatcacgccg ccgacggtgc cgccgatgcc
     5401 gcgcgcatag ccgagggcct tctgcgccgc cttgccggtg acgtcgcggt cgaccgcgaa
     5461 cagcgacggc acgccgcggc cgcgctgata ttcgcggcgg acgagatcgc ccggaccctt
     5521 gggcgcgacc agaatgacgt ccatgtcggc gcgcggcacg acgcggccgt agaggaccga
     5581 gaagccgtgc gcgaacagaa gcgccacgcc cttgggcgcg ttcggctcga tcacgtcctt
     5641 gtagagatcg gcgtgcgtca tgtccggcgt caggatggcg atgatggtcg cctccttgca
     5701 ggcgtcggcc accgacagga cggtgtggcc gtcggccttg gccttgacct cgccggcgcc
     5761 gccggggcgc aggcccacca cgacgtccgc gcccgaatcc ttcaggttcg aggcgtgggc
     5821 gcggccctgg ctgccgtaac cgatgatggc gatacgctca cccttcaggc tgtcgggcga
     5881 aacgtcgtca ttcgtgtaga tcttcacagt ggtctccgta ggtctgggat agcttaagat
     5941 tttgggatag gttggcttat gtatgcgccg ccgtaacaag gcgggcgtat ttctgcaagg
     6001 ccccggcgcc gaaaacggcc cggcccggct tctgcgcgcg gacgctgcgg cgcaggtcga
     6061 tatcggccat gacgtcgatg cggcgggcgg aaaggtcgat atggatgatg tcgtcgtcgt
     6121 tgacataggc gatcgggccg cgcgccgccg ccgacggggc gacctggccg atgaccgcgc
     6181 cgtcgctcag cggcgcatcc gtgatcagga cgacgcgctc cagcccggcg gcgtccagcg
     6241 ccgactgaac ggcgccggcg tcgaaggccg cgtcgtcgcg cacgatcagg atgtcgcagg
     6301 accggacccg gcccgccgcg atcccggcca cggcttcggc gccgctgtcg aaaacgcgcg
     6361 ccggaccgtc gaaaaccgct tcgtcgaacg cgtcgagccg cgccacgcag ccttccggcg
     6421 ccagcgcgcc gtaaaggatc gcgaagccgc cgaccgcgcc gcgcaccaca tcgcccgcgc
     6481 catgataggg agcggcgttg taacggttcg gattggcgat ggcgttcatg gcgatgactc
     6541 tacagattca gatgtacgaa aaaatcccgc tctcggcatg gagcgggagg attttggtcc
     6601 tcattaggcg ggtttaaacc tgcgctacca ctccggcatg aaggggcgaa gcacgccccc
     6661 gctaagcagc gatacgatgt cgagtacaag caggacgaaa agcgccgacg acgccagggc
     6721 gacgaaccct tgcgaagcgt cttgcttcat cgcgatgacc ttgactttcg acataaaacc
     6781 gtaactccgc gccaccgatg gcggttgcgt gtggatatat aatcgcaagc ggcgccgaaa
     6841 tgcaaactga aattccgccg aaattggaaa atttttccgt cgcgaagcgg ttttacggcg
     6901 gcgcagcaaa atattatttc acttaatggc catgaatggt gaatatattt cgcattgcat
     6961 tgcaaaatgc cggaaatcac ggctttgtga ggccgaatcg cgaaacattc tgtcttggcg
     7021 gcccgcgccc ttgcggcaaa cgcgccttgc cacctccgca cgggggtcta tataggtcgg
     7081 accgacgcct cgcgtcctgc gattttccac gtacaggttc aagaccatgc ccgcctatcg
     7141 ttcccgcacc accaccttcg gccgcaacat ggcgggcgcg cgcgcgctct ggcgcgccac
     7201 cggcatgaag gacggcgact tcaccaagcc gatcgtcgcc atcgccaact ccttcaccca
     7261 gttcgtgccc ggccacgtcc acctgaagga tctggggcaa ctggtggcgc gcgagatcga
     7321 acgcgcgggc ggcgtcgcca aggaattcaa caccatcgcg gtcgacgacg gcatcgccat
     7381 gggccacggc ggcatgctct attccctgcc ctcgcgcgac ctgatcgccg acagcgtcga
     7441 atacatggtc aacgcccact gcgccgacgc gctggtgtgc atctccaact gcgacaagat
     7501 cacgccgggc atgctgatgg cggccatgcg gctcaacatt ccgaccatct tcgtgtccgg
     7561 cgggccgatg gaggcgggca aggtggtgtg gccggacgaa agcggcgtgg ccagggaaca
     7621 cgcgctcgac ctgatcgacg ccatgatgca ggcgggcgac gaccgcgttt ccgacgcgca
     7681 ggtgcaggcg gtcgaggaag ccgcctgccc gacctgcggc tcgtgttcgg gcatgttcac
     7741 cgccaattcg atgaactgcc tgaccgaggc tctgggcttg agcctgcccg gcaacggcac
     7801 gaccctggcc acccattcgg accgcgagcg cctgttctgc gaggcgggcc gcaccatcgt
     7861 cgatctggcc gagcgctggt acgagcagga cgacgcctcg gtgctgccgc gctcgatcgc
     7921 cagcaaggaa gccttcgaga acgcgatggc gctggacgtc gccatgggcg gctcgaccaa
     7981 caccgtgctg catatcctgg ccgccgcgca ggaggcgggc gtcgacttca ccatggccga
     8041 catcgaccgc atctcgcgca acacgccgtg catctgcaag gtggcgccgt cggtcgccaa
     8101 cgtgcatatc gaggacgtgc atcgcgcggg cggcatcatg ggcattctgg gcgagctgaa
     8161 tcgcggcggc ctgatccacg gcgagcagcc gaccgtgcat gcgccgtcgc tggcggcggc
     8221 gctggcgcac tgggacgtca agacctcgaa cgattcctcg gtgtccgaat tcttcaaggc
     8281 ggcgccgggc ggcgtgcgca cgacccaggc cttctcgcaa tcgaagcgct ggagcgaact
     8341 ggacaccgac cgtcagaagg gcgtgatccg ctcggtcgaa cacgccttca ccaaggacgg
     8401 cggcctcgcc gtgctgtacg gcaacatcgc cgagaagggc tgcatcgtga agaccgccgg
     8461 ggtcgatgaa tcgatctgga aattcaccgg caaggcgcgc gtgttcgagt ctcaggacgc
     8521 ggcggcggcg gcgatcctca acaatcaaat tcaggcgggc gacgtggtgg tgatccgcta
     8581 cgaaggcccc aagggcggcc ccggcatgca ggaaatgctg tacccgacca cctatctgaa
     8641 atcgaa
//
LOCUS       DASVLL010000618         4950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_419014,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000618 DASVLL010000000
VERSION     DASVLL010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419014"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..727)
                     /locus_tag="VF402_10160"
     CDS             complement(44..727)
                     /locus_tag="VF402_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7800704.1"
                     /translation="MSDIPISLFVAPVTPLQQNCTVVWCNATRACAIIDPGGDLDPIL
                     HEIGRRGLRLDKIWVTHGHADHAGATAELKRRTGAVIEGPHRDDRFWIDRIPEDAARW
                     GLEGESFTPDRWLEDGDEVRLGETVWKVIHCPGHTPGHVVFYQPDSQFAQVGDVLFRG
                     SIGRTDFPRGNHADLISAITRKLWPLGDVRFVCGHGPMSSFAAERQSNPFVADSVIAR
                     AGPNTHGPG"
     gene            914..2494
                     /locus_tag="VF402_10165"
     CDS             914..2494
                     /locus_tag="VF402_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7800705.1"
                     /translation="MWRIKPLDAILATAEKKSLHRSLGPVQLTLLGIGAVIGTGIFVL
                     TATAAQKAGPGMMVSFMIAGLVCALAALCYSELASMVPVAGSAYTYSYAVMGELVAWL
                     VGWALILEYALGASAVAVGWSGHIVGFLDSVGLHLPHALTVGPPISIGFISGGEIGGL
                     INLPAVIVTVVVTALLVIGTRESATANAVLVAIKVAALTLFIVLTLPMITGHMSHFQP
                     FAPRGWGNPLGSSGTGVLGAAASIFFAYVGFDAVSTAAEETKNPQRNVPIGLIASLLI
                     CTIFYLLVAGGVIGSFGAQPLMDPATGQYYADGSPALYASAACQAVHAPTVCSKEALA
                     YVLRQVTNPLFGDIVGIAATIALPSVVLLMMYGQTRVFFVMAHDGLLPKGLSAVHPKF
                     RTPWVITLVTGVFVAIAAAFLPVGSLADYSNAGTLFAFAMVSIGVMILRFTDKNRPRP
                     FRTPGLFVIAPLSIAGCLLLFFSLGKESKEVFFGWTALGLILYFAYGFWKSNVRRGVV
                     EVHELDDDIPPPSVPPIA"
     gene            2553..>4950
                     /gene="hrpB"
                     /locus_tag="VF402_10170"
     CDS             2553..>4950
                     /gene="hrpB"
                     /locus_tag="VF402_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEX7800706.1"
                     /translation="MLPVEEIFPALKAHLAGVSNTALIVAPPGAGKTTGVPLALLDAP
                     WLDGRSIVLLEPRRLAARAAAARMAETLGEKIGETVGFRVRGESRVSAKTRIEVVTEG
                     IFSRRILDDPGLKDVACVIFDEFHERSLDADLGLAFARDAQKLLREDLRLIVMSATLD
                     GDRVLELLPEAGRFESQGRTFPIETHYLGRDQSLLLEADVARHAARLAAKLAPERAET
                     MLVFLPGQGEIHRVARHLDEIGLPPHIEVHKLYGAMEFRDQQAALAKNAPGRPKIVLA
                     TAIAETSLTLDKAAMVIDCGLSRLGRFDPARGVTRFLTERVSRASADQRRGRAGRTQA
                     GDAYRLWDAEQDRSLIPFARPEILETDLSQLALSLRLWGAKSTEGLALLDHPPAAAMR
                     EAVKLLQVLEALDAQGDLTAHGRLMARLPMAPRLAHMLLKAAENGAAVEGAQLAVLLS
                     ETGVGGRSTDLDARLEALGRDGSPKIASARRLAEGWAKSAAALVKDKPKQAGGRKFSH
                     HLLAECFPERVAKARGKPGEFLMANGRGCYVDAHDALARADYIAVGDLGGGKDRDRVL
                     LGAALTADEVRRLFAGRLETRPVLQRGGSGFRAFEETRLGALVLASKPLDKVPPQLLL
                     EAEAEEVRAKGLKALKFSEYAENLRARVAFLRAGDAAWPDMSDAALLSGLADWLGPYA
                     AGRSMLGLGADAVTQGLKGMLDHAQQRALDKLAPETLRLPTGSNARIDYAAEGGPRVE
                     ARVQELYGVTAHPTVGPGRTPLTLALLSPAHRPIQITKDLPAFWDGSWREVRAEMKGR
                     Y"
BASE COUNT          727 a         1534 c         1851 g          838 t
ORIGIN      
        1 tcataaccgt cgccaactgg ctatccgttt atgaggttgt gaactagccg gggccatgcg
       61 tattggggcc ggctctggcg atgacggaat cggcgacgaa cggattggac tggcgctcgg
      121 cggcgaagct cgacatcggg ccgtggccgc agacgaagcg cacgtcgcct aaaggccaga
      181 gtttgcgtgt gatggcagag atcaggtcgg catgattgcc gcgcgggaag tcggtgcggc
      241 cgatggagcc gcggaacagg acgtcgccga cctgcgcgaa ctggctgtcg ggctggtaaa
      301 agacgacgtg gccgggcgtg tggccggggc agtggatgac cttccagacc gtttcgccca
      361 gccggacctc gtcgccgtcc tccagccagc ggtcgggcgt gaagctttcg ccttcgaggc
      421 cccagcgcgc ggcgtcctcc gggatgcggt cgatccagaa ccggtcgtcg cggtgcgggc
      481 cttcgatcac ggcgccggtg cggcgtttaa gctccgccgt cgcgcccgca tggtcggcat
      541 ggccgtgcgt gacccagatc ttgtccagcc tcagcccgcg ccgtccgatc tcgtgcagga
      601 tcgggtcgag atcgccgccc ggatcgatga tcgcgcacgc cctggtcgcg ttgcaccaca
      661 cgacggtgca attctgttgc agcggcgtga cgggggcgac gaacaggctg atcggaatat
      721 cggacatggg caactcgcgg ataggggcgc agcggttatg gcgcgtccgt ccgacgcgcg
      781 caaccggcgt tcggcaaagg gagttgcgga tttcatgcat gcgttatacg ctcgtaacga
      841 tcgcgcctgc gccggagcga aaagggggct gtaggggctt gaaaaaattc aacaaaaagg
      901 gtgaaaaatg agcatgtggc gcataaagcc gctggacgcg atcctggcga cggccgagaa
      961 gaaatcgctg caccggtcgt tggggcccgt tcagttgacc ctgctgggga tcggggcggt
     1021 gatcggcacg gggatcttcg tgctgaccgc gacggcggcg cagaaggccg ggccgggcat
     1081 gatggtctcg ttcatgatcg cgggtctggt ctgcgccctg gcggcgctgt gctactccga
     1141 actggcctcg atggtgccgg tggcggggtc ggcctacacc tattcctacg cggtgatggg
     1201 cgaactggtg gcctggctgg tcggctgggc cttgattctg gaatacgcgc tgggggccag
     1261 cgccgtggcg gtcggctggt ccgggcatat cgtcggcttt ctggacagcg tcggcctgca
     1321 cctgccgcac gccctgacgg tggggccgcc gatctccatc ggcttcatca gcggcggcga
     1381 gatcggcggc ctgatcaacc tgccggcggt catcgtcacc gtggtggtga cggcgctgct
     1441 ggtcatcggc acgcgcgaaa gcgccacggc caacgccgtg ctggtggcga tcaaggtcgc
     1501 ggccctgacc ctgttcatcg tgctgaccct gccgatgatc accggccata tgagccattt
     1561 tcaaccgttc gcgccgcgcg gctggggcaa tccgctcggc tccagcggca cgggcgtgct
     1621 gggggcggcg gcgtcgatct tcttcgccta tgtcggcttc gacgcggtgt ccactgccgc
     1681 cgaggaaacc aagaacccgc agcgcaacgt gccgatcggc ctgatcgcgt cgctgctgat
     1741 ctgcaccatc ttctacctgc tggtcgcggg cggggtgatc ggctcgttcg gcgcccagcc
     1801 tttgatggac ccggcgacgg ggcagtatta cgccgacggc tcgcccgccc tgtacgccag
     1861 cgcggcgtgt caggcggtcc acgcgccgac ggtctgctcg aaggaggcgc tggcctatgt
     1921 gctgcgtcag gtcacgaatc ctttgttcgg cgacatcgtc ggcatcgcgg cgaccatcgc
     1981 cctgccgtcg gtcgtgctgc tgatgatgta cggccagaca cgcgtcttct tcgtgatggc
     2041 gcacgacggc ctgctgccca aggggttgtc ggcggtgcat ccgaagttca ggacgccgtg
     2101 ggtgatcacc ctggtcaccg gcgtgttcgt ggccatcgcc gccgccttcc tgccggtcgg
     2161 gtcgctggcc gactattcca acgcgggcac gctgttcgcc ttcgccatgg tgtcgatcgg
     2221 ggtgatgatc ctgcgcttca ccgacaagaa ccggccgcgt ccgttccgca cgccgggcct
     2281 gttcgtcatc gcgcccttgt ccatcgcggg ctgcctgctg ctgttcttca gcctgggcaa
     2341 ggagtcgaag gaggtcttct tcggctggac ggcgctgggc ctgatcctct acttcgccta
     2401 cggcttctgg aagtcgaacg tgcggcgcgg cgtggtcgaa gtgcatgaac tggacgacga
     2461 tattccgccg cccagcgtgc cgccgattgc gtaagcgctt tgcggaaaca ggaaaccgcc
     2521 ctaaatgagg gcggtttttt gttggacgac ccatgctgcc cgtcgaagag atatttcccg
     2581 cgctcaaggc gcatctggcc ggagtttcca acacggcgtt gattgtcgcg ccgccggggg
     2641 cgggcaagac gaccggcgtg ccgctggccc tgctggatgc gccgtggctt gacgggcgct
     2701 ccatcgtgct gctggagccg cggcggctgg cggcgcgggc ggcggcggcg cgcatggccg
     2761 agactctggg cgaaaagatc ggcgagacgg tcggcttccg ggtgcggggc gaaagccggg
     2821 tgagcgcgaa aacgcgcatc gaggtggtga ccgagggcat attcagccgc cgcatcctcg
     2881 acgatccggg gcttaaggac gtggcctgcg tgatcttcga cgagttccac gaacgcagcc
     2941 tcgacgccga tctgggcctc gccttcgcgc gcgacgcgca gaagctgctg cgcgaggatc
     3001 tgaggctgat cgtgatgtcg gcgacgctgg acggcgaccg ggtgctggag ctgttgccgg
     3061 aggccgggcg cttcgagtcg cagggccgga ccttccccat cgagacgcac tatctggggc
     3121 gcgaccagag cctgttgctg gaggccgatg tcgcgcggca cgcggcgcgg ctggcggcga
     3181 aactggcgcc ggagcgcgcc gagaccatgc tggtcttcct gcccggacag ggcgagattc
     3241 accgcgtcgc gcgtcatctc gacgagatcg gcctgccgcc gcatatcgag gtgcataagc
     3301 tttacggcgc gatggagttc cgcgaccagc aggcggcgct ggcgaaaaac gcgccggggc
     3361 ggccgaagat cgtgctggcc accgccatcg ccgagacctc gctgacgctg gacaaggcgg
     3421 cgatggtgat cgattgcggg ctgtcgcgtc tgggccggtt cgatccggcg cgcggcgtga
     3481 cgcgcttcct taccgagcgc gtgtcgaggg cgtcggcgga ccagcggcgc ggccgcgcgg
     3541 gccggacgca ggccggcgac gcttaccgcc tgtgggacgc cgagcaggac cggtcgctga
     3601 tcccgttcgc gcggccggag attctggaga ccgacctgtc gcaactggcg ctgtccttac
     3661 gcttgtgggg cgcgaaatcg accgagggtc tggccctgct cgatcatccg cccgccgccg
     3721 ccatgagaga ggcggtgaag ctgttgcagg tgctggaggc gctggacgca cagggcgatc
     3781 tgaccgcgca cgggcgcctg atggcgcggt tgccgatggc gccgcgtctg gcgcacatgc
     3841 tgctgaaggc cgccgagaac ggcgcggcgg tcgagggcgc gcaactggcc gtgctgctga
     3901 gcgagaccgg ggtcggcggc aggtcgaccg atctcgacgc gcggctggag gcgctgggcc
     3961 gcgacggatc gcccaaaatc gcctcggcgc ggcggctggc cgagggctgg gcgaagtcgg
     4021 cggcggcgct cgtgaaagac aagccgaaac aggcgggggg acgcaaattc tcacaccatc
     4081 tgttagccga atgtttcccc gaacgggtgg cgaaggcgcg cggcaagccg ggcgaatttc
     4141 tgatggccaa cgggcgcggt tgctatgtcg acgcgcacga cgcgctggcg cgggccgact
     4201 atatcgcggt cggcgatctg ggcggcggca aggaccgcga ccgcgtgctg ctgggggcgg
     4261 cgctgacggc ggacgaggtg cgccgtctgt tcgccgggcg gctggagacg aggccggtcc
     4321 tgcaacgcgg cgggtcgggg ttccgcgcgt tcgaggagac gcggctgggc gcgctggtgc
     4381 tggcgtcgaa gccgctcgat aaggttccgc cgcagcttct gcttgaggcc gaggctgagg
     4441 aggtgcgcgc caagggcttg aaggcgctga aattttccga atacgccgag aacttgcgcg
     4501 cgcgggtggc gtttttgcgc gccggggatg cggcatggcc ggatatgtcg gacgcggcgc
     4561 tgctgagcgg gctggccgac tggctggggc cctatgcggc ggggcggtcg atgctggggc
     4621 tgggcgccga tgcggtgacg caaggcttga aggggatgct cgaccatgcg cagcagcggg
     4681 cgctggacaa gctggcgccg gaaacgctgc ggctgccgac cgggtcgaac gcgcgcatcg
     4741 actatgcggc ggagggcggg ccgcgcgtcg aggcgcgggt gcaggagctt tacggcgtga
     4801 cggcgcatcc gaccgtgggg ccggggcgca cgccgctgac tttggccctg ctgtcgcccg
     4861 cgcaccggcc gatccagatc acgaaagacc tgcccgcctt ctgggacggt tcgtggcgcg
     4921 aggtgcgcgc cgagatgaag ggccgctatc
//
LOCUS       DASVLL010000619         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_41810, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000619 DASVLL010000000
VERSION     DASVLL010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41810"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF402_10175"
     CDS             complement(<1..187)
                     /locus_tag="VF402_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEX7800707.1"
                     /translation="MNNLHRELAPISDDAWAQIEQEAARTLKRYLAARRVVDTSEPQG
                     MDLAAVGAGHTRAIDGPG"
     gene            complement(184..1215)
                     /locus_tag="VF402_10180"
     CDS             complement(184..1215)
                     /locus_tag="VF402_10180"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX7800708.1"
                     /translation="MPASAVPQAVTAGLTRAAIFVVVTLNAGADAESAVRGLCADMSS
                     LVRGVGFRAQGGGLSCVVGIGRAAWDRVFGAPGPKELHPFREILGVHHAPSTPGDLLF
                     HIRAARMDLCFELAAQIRTRLAGAAAAIDAVQGFKYFDDRDLLGFVDGTENPAAQAAV
                     AATLIADEDPAFAGGSYVIVQKYLHDLAKWDAIPVEQQEKIIGRRKLSDIELADADKP
                     SYAHNVLTSLEENGAAVQILRDNMPFGDIGRGEFGTYFIGYARSPRPIERMLENMFVG
                     APPGNYDRLLDVSRAVTGSLFFAPTADFLDAADAAPASVPAAADPQLAKPQRDGSLRI
                     GSLKDEAIL"
     gene            complement(1319..2230)
                     /locus_tag="VF402_10185"
     CDS             complement(1319..2230)
                     /locus_tag="VF402_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7800709.1"
                     /translation="MFIRQLNYLIALDQHRHFGRAAESCHVSQPALSNGIRELERELG
                     ITIVRRNRSFEGVTPEGERVILWVRQVLASLDGLRQEADLIRSVPRGHLAIGVIPTAG
                     HGATLLSAEYRQILPQLTLEVLALSTPDILQRLKSQDIHLGMLYDRSVADESYDVLPL
                     FSERYVLIASEQTSLPHTLGWAEVADLPLCLLSRDMQNRQTLEELFRKVEARPNIVLE
                     TNALRVLLTECQSGRAFSVIPLSALPGVPALENLRAHPITPEHAEDVCLVRLRRETQP
                     ALSVAAWQIAANLDLEGVLNKSLVHPS"
     gene            2420..3328
                     /locus_tag="VF402_10190"
     CDS             2420..3328
                     /locus_tag="VF402_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HEX7800710.1"
                     /translation="MILRDRPSMWKLFFVMQGSIVPKILPQVAVIGGLSALVVLIHLR
                     WPHFLPIYDGGPFALLGIAISVFLSFRNSACYDRWWEGRKQWGALIRLSRDMARQTLI
                     LPEADRRRMLDLVCAFAHALVQRLRPEETERDKVVCRLSAIEADQWQESAFPTDYILR
                     RMGALLAEWRAAGRIGDIVFQILDRTISGMAGVQAACERIRSTPVPFPYMLLLNRTAL
                     LFCLCIPFGFAHTLGWATPFPTMLIAYTFFGLDKLGDELEDPFALRDNGLPLSFLAQS
                     IEVNMREALGETELPVLPVPDGYVLR"
     gene            3461..>4692
                     /locus_tag="VF402_10195"
     CDS             3461..>4692
                     /locus_tag="VF402_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7800711.1"
                     /translation="MPDDKPRYKTYHHPAGGWGAANATAKVLLEQSVVAKGSRALLAM
                     NQPGGFKCPSCAFPDPACKKTLEFCENGAKALAHEATKFRVTRDFFARHTVAELMAQS
                     DYWLEMQGRLTEPMRYDAATDTYVPCSWDEAFAMIGRHLRGLKSPHEAEFYTSGRTPN
                     EAAFLYAIFVREFGTNNFPDCSNMCHEPTSRGLPPAIGVGKGTVIMEDFEHAEAIFII
                     GQNTGTNSPRMLTNLVEARKRGVPIVAVNPMPERALIRFAEPQDVVQMATFGSTPIAS
                     EFVHIRIGGDLALIKGMMKVMFEREAAGESVLDHDFIRDHTTGFETVRDEVMSLGWPE
                     LVAASGIDEAQIRRCAEIYIRSKATMICYGMGVTQHQQGSRLVQQVANLLFLRGNFGR
                     PGAGISPIRGHSNVQGDRT"
BASE COUNT          762 a         1542 c         1606 g          782 t
ORIGIN      
        1 cgcccggccc gtcgatcgcc cgcgtatggc ccgcgccgac cgccgcgagg tccatgccct
       61 gcggttcgga cgtatccacg acgcggcggg cggccagata gcgcttgagc gtgcgcgccg
      121 cctcctgttc gatttgcgcc caggcgtcgt ccgagatcgg cgccagttca cggtgcaggt
      181 tgttcatagg atcgcctcgt ctttcaagga gccaatgcgc agcgaaccgt cccgctgcgg
      241 ttttgcgagt tgcgggtccg ccgccgccgg aacggacgcc ggagccgcgt cggcggcgtc
      301 cagaaaatcg gccgtgggcg cgaagaacag cgacccggtc acggcgcggc tgacgtcgag
      361 caggcggtcg taattgccgg gcggcgcgcc gacgaacatg ttttccagca tgcgctcgat
      421 cgggcgcggc gagcgggcgt agccgatgaa ataggtgccg aactcgcccc tgccgatgtc
      481 gccgaagggc atgttgtcgc gcagaatctg cacggcggcg ccgttctcct caaggctggt
      541 cagcacgttg tgggcgtagc tcggcttgtc ggcgtcggcc agctcgatgt cggagagctt
      601 cctgcggccg atgatctttt cctgctgctc gaccgggatc gcgtcccact tggcgaggtc
      661 gtgcaggtat ttctgcacga tgacgtagct gccgcccgcg aacgcggggt cttcgtcggc
      721 gatcagggtc gcggcgacgg cggcctgcgc cgccgggttc tcggtcccgt cgacgaagcc
      781 cagcaggtcg cggtcgtcga aatatttgaa gccctgcacc gcgtcgatgg ccgcggcggc
      841 gcctgcgagc cgcgtccgga tctgcgccgc cagttcgaag cacaggtcca tgcgcgcggc
      901 gcggatatgg aacagaaggt cgccgggcgt cgaaggcgcg tgatgcacgc cgaggatttc
      961 gcggaaggga tgcagctcct tcggtcccgg cgcgccgaac acgcggtccc aggccgcccg
     1021 gccgatcccg acgacgcagg acaggccgcc gccttgcgcc cggaagccga cgccgcgcac
     1081 cagggacgac atgtcggcgc acaggccgcg cacggcggac tccgcgtccg cccctgcgtt
     1141 gagggtcacg accacgaaga tcgcggcgcg ggtcagtccg gcggtgacgg cttgcggaac
     1201 ggcggatgcg ggcacgcgat atccttattc aagttcgggg cggagaaatg tcagaagctg
     1261 cggtcagcgg gccggcaggt ccagtcgatt gcctgaatat cgtgataatc tatccttatc
     1321 atgaaggatg gaccagggat ttgttgagca cgccctcgag gtcgagattg gcggcgattt
     1381 gccaggcggc gaccgacagg gccggttggg tttcgcgccg cagccggacg aggcacacgt
     1441 cctcggcgtg ttccggcgtg atcggatggg cgcgcaggtt ctccagggcc gggacgccgg
     1501 gcagggcgct cagcgggatg acgctgaagg cgcggccgct ctgacattcg gtcagcagca
     1561 cgcgcagggc gttggtctcc agcacgatgt tcggcctggc ctcgaccttg cggaacagtt
     1621 cctccagcgt ctggcggttc tgcatgtcgc ggctgagcag gcacaggggc aggtcggcca
     1681 cttcggccca gccgagcgtg tgcggcaggg aggtctgctc gctggcgatc aggacgtagc
     1741 gctccgagaa caggggcagg acgtcgtagg attcgtcggc caccgaacgg tcgtagagca
     1801 tgcccagatg gatgtcctgc gatttcaggc gctggaggat atccggcgtg ctgagggcca
     1861 gcacttcgag cgtgagttgc ggcaggatct ggcggtattc ggcgctgagc agggtggcgc
     1921 cgtgcccggc cgtggggatg acgccgatcg cgagatgccc gcgcgggacg ctgcggatca
     1981 ggtcggcctc ctgccggagc ccgtccagcg aggccagaac ctgacgcacc cacaggatca
     2041 cgcgctcgcc ttcgggcgtg acgccttcga aggaccggtt gcgcctgacg atggtgatgc
     2101 caagctcgcg ctccagctcg cggatgccgt tcgacagggc gggctgcgac acgtggcagc
     2161 tttcggcggc gcgtccgaag tggcggtgct gatccagcgc gatcaggtag ttcaactggc
     2221 gaatgaacac ggggcgcctg cttccgggac ggcgtgatgg acgccgttac cctagcctga
     2281 taatccggga ttatcaaaac tttcagcaaa tgaattggac cggccttccc cgaaagctcc
     2341 actctccgcc gggcgtcggc gggcggcgcg tggcgggaaa gttttgcagc cgaaggacgc
     2401 cagacgaggt gactgaacaa tgattctccg cgaccgcccc tcgatgtgga agctcttttt
     2461 cgtgatgcag ggttcgatcg tgccgaaaat cctgccgcag gtcgccgtca tcggcggcct
     2521 gtccgcgctg gtcgtgctga ttcatctgcg ctggccgcac ttcctgccga tctatgacgg
     2581 cgggccgttc gccctgctgg gcatcgccat ttccgtgttc ctcagcttcc gcaacagcgc
     2641 ctgctacgac cgctggtggg aggggcgcaa gcaatggggc gcgctgatcc gcctgtcgcg
     2701 ggacatggcg cgccagaccc tgatcctgcc cgaagccgac cgcaggcgca tgctcgatct
     2761 ggtctgcgcc ttcgctcacg ccctggtcca gcgcctgcgc ccggaagaaa ccgagcgcga
     2821 caaggtggtc tgccggctga gcgcgatcga ggccgaccaa tggcaggaaa gcgcgtttcc
     2881 gaccgactat atcctgcgcc gcatgggcgc cctgctggcc gaatggcggg cggcgggccg
     2941 gatcggcgat atcgtgttcc agattctcga ccgcacgatc agcggcatgg cgggcgttca
     3001 ggccgcctgc gagcgcatcc ggtcgacgcc cgtgccgttt ccctacatgc tgctgctcaa
     3061 ccgcaccgcc ctgctgttct gcctctgcat cccgttcggg ttcgcgcata cgctgggctg
     3121 ggcgacgccg tttccgacca tgctgatcgc ctacaccttc ttcggcctgg acaagctcgg
     3181 cgacgaactg gaagacccgt tcgccctgcg cgacaacggc ctgccgctgt cgttcctggc
     3241 gcaatcgatc gaggtcaata tgcgcgaggc gctgggggag acggaattgc ccgtcctgcc
     3301 cgtccccgac ggctatgtgc tgagataggg atgatgcgcc gccctcacaa ttctttgacc
     3361 gcgaaccggt cttcacgcac cttcgcagac ggggcgcgca agggtagagt ccgccgcgcg
     3421 gcccgcgata acgcacgtcg ccggagtaag ggagatggcc atgcctgacg acaagccgcg
     3481 ctataaaacc tatcatcatc cggcgggcgg ctggggggcg gccaacgcca ccgccaaggt
     3541 gctgctggag cagagcgtgg tcgccaaggg ctcgcgcgcc ctgctggcga tgaaccagcc
     3601 cggcggcttc aagtgcccaa gctgcgcctt tcccgatccg gcctgcaaga agacgctgga
     3661 attctgcgag aacggcgcca aggcgctggc gcacgaggcg acgaagttcc gcgtcacgcg
     3721 cgacttcttc gcgcggcaca ccgtcgccga gctgatggcg cagtcggact actggctgga
     3781 aatgcagggc cgcctgaccg agccgatgcg ctacgacgcg gcgacggaca catatgtccc
     3841 ctgctcgtgg gacgaggctt tcgcgatgat cggacgccac ctgcgcggcc tgaaaagccc
     3901 gcacgaggcc gagttctaca cctccgggcg cacgcccaac gaagcggcct tcctctacgc
     3961 catcttcgtg cgcgaattcg gcaccaacaa cttccccgac tgctccaaca tgtgccacga
     4021 gccgaccagc cgcggcctgc cgcccgccat cggcgtcggc aagggcacgg tgatcatgga
     4081 ggatttcgaa cacgccgagg cgatcttcat catcggccag aacaccggca ccaattcgcc
     4141 gcgcatgctg accaatctgg tcgaggcgcg caagcgcggc gtgccgatcg tggcggtcaa
     4201 tccgatgccg gagcgcgccc tgatccgctt cgccgagccg caggacgtgg tgcagatggc
     4261 caccttcggc tcgaccccga tcgccagcga gttcgtgcat atccgcatcg gcggcgacct
     4321 cgccctgatc aagggcatga tgaaggtcat gttcgagcgc gaggcggcgg gcgaaagcgt
     4381 gctcgaccac gatttcatcc gcgaccacac caccggcttc gagaccgtgc gcgacgaggt
     4441 gatgagcctc ggctggccgg agctggtcgc ggcgtccggg atcgacgagg cgcagatccg
     4501 ccgctgcgcc gagatctata tccgctcgaa agccaccatg atctgctacg gcatgggcgt
     4561 aacccagcac cagcagggct cgcgtctggt gcagcaggtg gccaacctgt tgttcctgcg
     4621 cggcaatttc ggccgtccgg gcgcgggcat ctcgccgatc cggggccatt ccaacgtgca
     4681 gggcgaccgc ac
//
LOCUS       DASVLL010000620         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_154142,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000620 DASVLL010000000
VERSION     DASVLL010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154142"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VF402_10200"
     CDS             <1..1002
                     /locus_tag="VF402_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX7800712.1"
                     /translation="NSKEIGATYGQVSPTTIATIQRNLLALENQGAAQFFGEPALRLE
                     DMMRTNIAGKGYVNVLAADKLMQTPKLYSTFLLWLMSELFEQLPEVGDPDKPRLVFFF
                     DEAHLLFDQASKALEDKIEQVVRLIRSKGVGVYFVTQNPADVPDTVLGQLGNRVQHAL
                     RAYTPAEKKGLKSAAASFRANPAFDTMEVLQQLGVGEALVSLLDAKGTPNVTERTLIR
                     PPASRLGPVTDAERAQIIAASPVAGVYDKAVDRESAFEVLSGRLAAAAAAAPPPPPTA
                     EEKAAAKAVADAEKAKQKAQDQMMKNVERVAVPIIRSVGIAIAGSLVRGLLGNTRRR"
     gene            1140..1502
                     /locus_tag="VF402_10205"
     CDS             1140..1502
                     /locus_tag="VF402_10205"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX7800713.1"
                     /translation="MRKGAKIRQARDFRKQMTHAEVLLWLGLKGHSHEGFTFRRQHPI
                     GPYIADFYCAKARLIIEVDGLIHERDDRPQRDALRDRWFAGQGVETLRIPAADIMADA
                     HDVSDGIVRYVRERLGSG"
     gene            complement(1499..2260)
                     /locus_tag="VF402_10210"
     CDS             complement(1499..2260)
                     /locus_tag="VF402_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7800714.1"
                     /translation="MIKTAPKTALVTGAAKRIGRAIALRLARDGYDIGVHYGRSRDDA
                     LALVAELEALGVRAAAVQADLAEVATVTRLIDDARAALGPVSVLINNASVFADDRARN
                     LTLDSWRLHMDTNLLTPVLLSQAFAAQADLPRPASIINLIDQRVLKPSPPFFSYGLSK
                     AGLWHATRTLAQALAPAIRVNAIGPGPTLKSIHQSEQDFAREARATLLQKPTSPEEVA
                     EAAVFLLNAPSVTGQMICVDSGQHLTWKTVDMLDL"
     gene            2430..>2850
                     /locus_tag="VF402_10215"
     CDS             2430..>2850
                     /locus_tag="VF402_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7800715.1"
                     /translation="MSTWKRRLEPFARPFFFFWSRLTRGKTLGVRALVTDQAGRVLLV
                     EHTYVPGWWLPGGGVDKGETAHQAVARELREEAGVDPVGEPRLISVHSNEAFFPGDHV
                     LLFRIIDWRPCAMTSHGEIRGVGFFAPDDLPQPMNAAS"
BASE COUNT          478 a          898 c         1013 g          461 t
ORIGIN      
        1 aattccaagg agatcggcgc aacctatggg caggtctcgc ccactaccat cgccacgatc
       61 cagcgcaacc tgctggcgct ggaaaaccag ggcgcggcgc agttcttcgg cgaaccggcg
      121 ctgcggctcg aggacatgat gcgcaccaac atcgccggca agggctatgt caacgtgctg
      181 gccgccgaca agctgatgca gacgccgaag ctctattcga cctttttgct gtggctgatg
      241 tcggaactgt tcgagcaact gcccgaagtc ggcgacccgg acaagccgcg cctcgtcttc
      301 ttcttcgacg aggcgcacct tctgttcgat caggcgtcca aggcgctgga ggacaagatc
      361 gagcaggtgg tgcggctgat ccgctccaag ggcgtgggcg tctatttcgt gacgcagaac
      421 ccggcggacg tgcccgatac ggtgctgggc cagctcggca accgcgtgca gcacgccctg
      481 cgcgcctata cgcccgccga gaagaagggg ctgaaatcgg cggcggcctc gttccgcgcc
      541 aatccggcct tcgacacgat ggaggtgttg cagcaactgg gcgtcggcga ggcgctggtg
      601 tcgctgctgg acgccaaggg cacgccgaac gtcaccgaac gcaccctgat ccgtccgccc
      661 gcgtcgcggc tggggccggt taccgacgcc gagcgcgccc agatcatcgc cgcctcgccg
      721 gtggcgggcg tctatgacaa ggcggtcgat cgcgaatcgg ccttcgaggt gctgagcggg
      781 cgtctggccg ccgccgcggc cgccgcgccg cccccgccgc cgacggcgga ggaaaaagcc
      841 gccgccaagg ccgtcgccga cgccgaaaag gccaagcaga aggcgcagga ccagatgatg
      901 aagaacgtcg agcgcgtcgc cgtgccgatc atccgctccg tcggcatcgc catcgccggc
      961 tcgctggtgc ggggattgct ggggaatacg cggcggcggt aaggggcggg gcggccccct
     1021 ccgtcagctt cgctgacacc tcccccgctt cgcgggggag gataatatgg tctgatcctc
     1081 ccctgcgaag cgggggaggt ggcgcggcca tttatggccg tgacggaggg gggcgtctta
     1141 tgcgcaaagg cgcgaaaatc agacaggcgc gcgatttccg caagcagatg acgcacgccg
     1201 aagtcttgct atggctgggg ctgaaagggc atagccatga aggcttcacc ttccggcgcc
     1261 agcatccgat tggcccttac atcgccgatt tttactgcgc gaaagccagg ctgatcatcg
     1321 aagtcgatgg cctgatccac gaacgcgacg atcgcccgca gcgggatgcg ttgcgtgatc
     1381 gatggtttgc cgggcaaggc gtcgaaaccc tgcggattcc cgctgcagac attatggcgg
     1441 atgcgcacga tgtcagcgac ggcattgtgc gctatgtccg cgagcggctg ggcagcggct
     1501 aaagatccag catatccacc gtcttccacg tcaggtgctg gccggaatcg acgcagatca
     1561 tctggccggt gacgctgggg gcgttcagca ggaagacggc ggcttcggcg acttcctccg
     1621 gcgaggtcgg cttttgcagc agggtggcgc gggcctcgcg ggcaaagtcc tgctccgact
     1681 ggtggatgga tttcagcgtc gggccgggac cgatggcgtt gacgcggatg gcgggcgcga
     1741 gggcctgcgc cagggtgcgc gtggcgtgcc acaggcccgc cttcgacagg ccgtagctga
     1801 agaacggcgg cgagggcttc agcacccgct ggtcgatcag gttgatgatc gaggccggtc
     1861 gcggcaaatc cgcctgcgcc gcgaacgcct gcgataaaag caccggcgtc agcaggttgg
     1921 tgtccatatg cagacgccac gagtcgagcg tcaggttgcg cgcgcggtcg tcggcgaaca
     1981 ccgaggcgtt gttgatcagc acggagacgg ggccgagcgc cgcgcgggcg tcgtcgatca
     2041 ggcgcgtcac cgtggcgacc tcggccagat cggcctgcac ggcggcggcg cgcacgccca
     2101 gcgcctcaag ctcggccacc agcgccagcg cgtcgtcgcg cgaccgtccg taatgcacgc
     2161 cgatgtcgta accgtcgcgc gccagtctga gggcgatagc gcggccgatc cgcttggccg
     2221 cgccggtgac gagtgctgtc ttcggcgcgg tcttaatcaa ggcgcccgaa gtcgacgagg
     2281 ttcaaaaggc ccatgacgat caggaaggcg gcgaaaatac cgatggcgag catagggacg
     2341 actccatatc tgttccgcta gggttaaacg ggcgcgggcg ccatggcaag aggcttgcga
     2401 gagggcgcga cacgcggcaa gttgcgccca tgtcgacatg gaagcgccgt cttgagccgt
     2461 tcgcgcggcc gtttttcttt ttctggtcgc ggctgacgcg cggcaagacg ctgggcgtgc
     2521 gcgccttggt gacggaccaa gcgggccggg tgctgctggt cgagcatacc tatgtgccgg
     2581 gctggtggct gcccggcggc ggcgtggaca agggcgagac ggcgcatcag gccgtggcgc
     2641 gcgagctgcg cgaggaggcg ggcgtcgatc cggtcgggga gccgcgcctg atctcggtgc
     2701 attctaacga ggcgttcttt cccggcgacc atgtgctgct gttccgcatc atcgactggc
     2761 ggccctgtgc gatgacctcg catggcgaga tccggggcgt cggctttttc gcgcccgacg
     2821 acctgccgca gccgatgaac gcggcctcgg
//
LOCUS       DASVLL010000621         6311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_312966,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000621 DASVLL010000000
VERSION     DASVLL010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312966"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /gene="ftsZ"
                     /locus_tag="VF402_10220"
     CDS             complement(<1..1036)
                     /gene="ftsZ"
                     /locus_tag="VF402_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7800716.1"
                     /translation="MAIHLSAPRATELKPRIVVFGVGGAGGNAVNNMIEAGLEGVEFV
                     VANTDAQQLQFSRTDARIQLGVGITQGLGAGAHPEVGMTAAEESADLIAEHLDGAHMV
                     FITAGMGGGTGTGAAPIIAKCARERGILTVGVVTKPFTFEGRHRMRLADAGISELQRY
                     VDTLIVIPNQNLFRIANERTTFAEAFGMADQVLHAGVRSITDLMVLPGLINLDFADVR
                     SVMSDMGKAMMGTGEASGEDRALLAAQNAIQNPLLDETSLKGAKAVLVNVTGGLDMTL
                     LEVDEAANAISDQVDPEANIIFGAAFDPSLDGMIRVSVVATGMDGASMAAIEPKATSR
                     TAPAPAPLLME"
     gene            complement(1414..2811)
                     /gene="ftsA"
                     /locus_tag="VF402_10225"
     CDS             complement(1414..2811)
                     /gene="ftsA"
                     /locus_tag="VF402_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX7800717.1"
                     /translation="MSGFEARQNAKQQALQPQLVKTVTPAQKALRAGAAPGERAPAAS
                     GLIAALDLGASKIGCFIMRPDGARHADHSIRVAGVGYVQSRGVRAGAIIDMEAASQAI
                     AQAVERAEAMANVSIQGVRVSVPGAGCAAHRVSAQVSLGLKPISDLDLNRAIASALAQ
                     ARFANRRAIHLLPVSWSVDGQVGVRDPRGLNGRNLGLELLVVSIDENVFNNIVQCVAQ
                     AHLEVHAIVCAPLASAVAALEEDEKDLGCICIDMGASSTTVAIFMGGTLIHVDSLNVG
                     GGHVTADIARGLSTTLAGAERLKTLHGSAIASSNEDRETVEAPPRGDDRGGAPIVVPR
                     SILKGIIAPRVEEIFELLREKIKASGVHVEPGAGIVLTGGASQLAGVRELAIRVFDRP
                     VRLGKPMRVPHLGDAAAGPAFAAPAGVILRTLYGPRDVVPVRKIMAAKVGPRDAPSGS
                     GNLVMRVIDWLKANL"
     gene            complement(2842..3720)
                     /locus_tag="VF402_10230"
     CDS             complement(2842..3720)
                     /locus_tag="VF402_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX7800718.1"
                     /translation="MGGGKAGASKPSGRARKPQAPAGKPRLLGAVPMTGEATGWLAVL
                     VIGGLLAAVLLTGHRAEALGRAALGFADDRMAFVGVKLENVRLVGVSDQAAPDIKKAL
                     AFHRGQPFAFLNLDQVRRDVENVGWVKSASVRRQFPGTLIVSVNERPRLAVWQYQGRD
                     QVVDDTGQIIPEAVASQFPDLPLIVGEGANETAADIIELLRARPQLMQRVFALQRVDT
                     RRWNILLKNGTLIKLPAVNQDQALNRLDALIAQQRVLDQGLAEIDLLDPTALVVVPLE
                     PRTTAAAPAKPAAPSA"
     gene            complement(3895..5520)
                     /locus_tag="VF402_10235"
     CDS             complement(3895..5520)
                     /locus_tag="VF402_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX7800719.1"
                     /translation="MRRLAGWALSAVLALAAMGAQAADVTLRVTGGALAGDRLADNSY
                     VFHAVPFAAPPLGDLRWRAPQDVVPWTGVRHETDSAPACLQIDYGWQTDMAAKSSEDC
                     LYLEVRTPDLHPQKPLPVMVYIHGGANRAGGGAGTVMAGFAGKGVIIVSLQYRLGVFG
                     FLSHPGLSREQGGHSGNYALMDQIKALAWVRDNIAQFGGDPGNVTIFGQSAGAEDVGM
                     LLAAPAARGLFQKAIEESGPPQFGLPARTLAQNEAMGVDLARFYTDAVPDSDAALAAL
                     REVPARRLQFAADRLPVPIADDGFIWLQAVIDGKVLTQSPRDAFRKGEQAKVPLIIGT
                     VAQELGLFGGPDAVHAEIVRQFGAHAAGAMAFYGVTATTEPPADPLYGDVAMQLSTDL
                     MMRCPSQWVAQVQTQAGPSVWSYQLDVNDGAGPPHHSSDLAFVMNPQPAGRTDAEWPR
                     LLDYWTQFAKPAIRTARALPAGRPSAPPGAISSSRPPGLWSATTCANRSAVIATRHNM
                     TALCRRRHYPFQNLTRRAIRYQSVKDVRAFMEA"
     gene            complement(5517..>6311)
                     /locus_tag="VF402_10240"
     CDS             complement(5517..>6311)
                     /locus_tag="VF402_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7800720.1"
                     /translation="EAHARAPSLSGRVATIGVGAPGSVSPRTGVMRNANSTWLNGKTF
                     REDLEAALQRPVRLANDANCLALSEAIDGAGRGRSNVFAIIVGTGCGGGLVIDHKLIE
                     GANGVAGEWGHMPLPWPKPEEYPGAKCWCGLHGCLETWVSGTGFARDFHAVTGNTLKG
                     EEIIAAMRAGDAEAEAAFDRLVDRLGRGMAVLVNMVDPDVFVLGGGLSNAPELYERLA
                     GKIRPYVFSDVWEAEIVPARWGDSSGVRGAAYLWAQGQHLKAEVHP"
BASE COUNT         1019 a         2187 c         2126 g          979 t
ORIGIN      
        1 gttccatcag caggggcgcg ggcgccgggg cggtgcggct ggtcgccttg ggctcgatgg
       61 cggccatcga cgcgccatcc atgccggtgg cgacgaccga aacgcggatc atgccgtcga
      121 ggctcggatc gaacgccgcg ccgaagatga tgttggcctc cggatcgact tgatcggaga
      181 tggcgttggc cgcctcatcg acttccagca gcgtcatgtc gaggccgccg gtcacgttga
      241 ccagcacggc cttggcgccc ttgagcgagg tttcgtcgag cagcgggttc tgaatggcgt
      301 tctgcgccgc cagcagggcg cggtcctcgc cggacgcctc gcccgtgccc atcatcgcct
      361 tgcccatgtc ggacatgacc gagcgcacgt cggcgaaatc gagattgatc aggcccggca
      421 gcaccatcag gtcggtgatc gagcgcacgc ccgcgtgcag cacctgatcg gccatgccga
      481 aggcttcggc gaaggtagtg cgctcgttgg cgatgcggaa caggttctgg ttcgggatga
      541 cgatcagcgt atcgacatag cgttgcagct cgctgatgcc cgcatcggcc aggcgcatgc
      601 ggtgacggcc ttcgaaggtg aagggcttgg tcacgacgcc gacggtcagg atgccgcgtt
      661 cgcgcgcgca tttggcgatg atcggcgccg cgccggtgcc ggtgccgccg cccatgccgg
      721 cggtgatgaa caccatgtgc gcgccgtcca gatgctcggc gatcaggtcg gcggattcct
      781 cggcggcggt catgccgact tcgggatgcg cgcccgcgcc caggccctgc gtgatgccga
      841 cgccgagctg gatgcgcgcg tcggtgcgcg agaactgaag ctgctgggcg tcggtgttgg
      901 cgacgacgaa ttcgacgcct tcgagcccgg cttcgatcat gttgttgacg gcgttgcctc
      961 cggccccgcc gacgccgaac acaacgatac gcggtttcaa ctcggtcgcg cgaggcgcag
     1021 aaagatgaat agccatggct ctaggatcaa cctttcacaa tctctttcca ctccggaagc
     1081 cagccttgcc gctgcgcctc tttcatagag agtcatgaga ctacgattat gcttaacgaa
     1141 tgattacccg ataacttgag tcgggcggtt ttaaagcggt tccggtaaca atttttaaga
     1201 gaggttaccg aagtatgacc gagcttaacc ggcaaatgag tccgaaggga gtcgccgccg
     1261 gccttttcac cgcgaaacgg cggaaagcat taacagcttt ttacgaaacc ggccccgccg
     1321 acgcggccgc gcgccgtcat gcgcgcgtca agaccgcgcc cgccccggaa caaatacaga
     1381 aacaaatacg gaataacggc ggcgcgccgc gaatcagaga ttggccttca gccagtcgat
     1441 cacgcgcatg acgaggttgc ccgagcccga aggcgcgtcg cgcgggccga ccttggccgc
     1501 catgatcttg cgcaccggca ccacgtcgcg cggcccgtag agggtgcgca ggatcacgcc
     1561 cgccggggcc gcgaaggccg ggcccgccgc cgcgtcgccc agatgcggca cgcgcatcgg
     1621 cttgcccagc cgcaccgggc ggtcgaacac gcggatggcc agttcgcgca cgcccgccag
     1681 ttgcgaggcg ccgccggtca gcacgatgcc cgcgcccggc tcgacatgca cgccggacgc
     1741 cttgatcttt tcgcgcagca actcgaagat ttcctcgacg cgcggcgcga tgatgccctt
     1801 gaggatcgag cgcggcacga cgatcggcgc gccgccgcgg tcgtcgccgc gcggcggcgc
     1861 ctcgacggtt tcacggtcct cgttgctgga cgcgatggcc gagccgtgca gcgtcttgag
     1921 ccgctccgcc cccgccagcg tggtcgacag gccgcgcgcg atgtcggcgg tgacgtggcc
     1981 gccgccgaca ttgaggctgt cgacatggat cagcgtgccg cccatgaaga tggccacggt
     2041 cgtcgacgag gcgcccatgt cgatgcagat gcagccgagg tctttttcgt cctcctccag
     2101 cgccgcgacg gccgaggcca gcggcgcgca gacgatggcg tggacctcga ggtgcgcctg
     2161 cgccacgcat tgcacgatgt tgttgaagac gttttcgtcg atgctgacga ccagcagttc
     2221 gaggccgaga ttgcgcccgt tcaggccgcg cggatcgcgc accccgacct gtccgtcgac
     2281 gctccacgac accggcagca ggtggatggc gcggcggttg gcgaagcgcg cctgggccag
     2341 cgccgaggcg atggcgcggt tcaggtcgag atcggagatc ggcttcagcc ccagcgacac
     2401 ctgcgccgac acccggtgcg cggcgcagcc cgcgcccggc acgctgacgc gcaccccctg
     2461 aatcgagacg ttcgccatcg cctcggcgcg ctcgacggcc tgggcgatag cctgcgaagc
     2521 ggcctccatg tcgatgatcg cgcccgcgcg cacgccgcgc gactgcacgt agccgacccc
     2581 ggcgacgcgg atcgaatggt cggcgtggcg cgccccgtcc gggcgcatga tgaagcagcc
     2641 gatcttcgag gcgccgaggt ccagcgcggc gatcagcccc gatgcggcgg gcgcccgttc
     2701 gccgggcgcg gcgccggcgc gcagggcctt ctgcgcgggc gtgacagtct tgaccagttg
     2761 cggctgcagg gcctgctgct tggcgttttg gcgggcttca aaaccggaca tgagtttcta
     2821 ctcgtcagtc tggagggcga tttaagcgct tggggcggcg ggtttggcgg gagcggcggc
     2881 ggtcgtacgc ggttcgagcg gcacgaccac cagcgcggtg ggatcgagaa ggtcgatttc
     2941 ggcgaggccc tgatccagca cgcgctgctg cgcgatcagc gcgtcgaggc ggttcagggc
     3001 ctgatcctga ttgacggcgg gcagcttgat cagcgtgccg ttcttcaaca ggatgttcca
     3061 gcggcgcgtg tcgacccgtt gcagggcgaa gacgcgctgc atcagttgcg ggcgcgcgcg
     3121 caagagttcg atgatgtcgg cggcggtttc gttggcgcct tcgccgacga tcagcggcag
     3181 gtcggggaat tgcgacgcca ccgcctcggg gatgatctgg cccgtatcgt cgacgacctg
     3241 atcgcggccc tgatattgcc agacggccag acgcgggcgc tcgttcaccg acacgatcag
     3301 cgtgccggga aactgacgcc ggacgctggc cgacttgacc cagccgacat tctcgacgtc
     3361 gcgccgcacc tgatcgagat tgaggaaggc gaaaggctgg ccgcgatgga aggccagcgc
     3421 cttcttgatg tccggcgcgg cctgatccga cacgccgacc agacgcacat tctccagctt
     3481 cacgccaacg aaggccatgc ggtcgtcggc gaaacccagc gcggcgcggc ccagcgcctc
     3541 ggcgcgatgt ccggtcagca acaccgccgc cagcagcccg ccgatcacca gcacggccag
     3601 ccagccggtc gcctcgcccg tcatcggcac ggcgcccaga aggcgcggct tgcccgccgg
     3661 ggcctgcggc ttgcgcgcgc ggccggaggg cttgctcgcg cccgccttgc cgccccccat
     3721 cttgccaccc tccgccttgc cgccgcgcga cgaaggctgc gaaggggcct ggcgtctgcc
     3781 tccacgaacg atggcgggca tactgatctc cgaacacgta catcggggcc ggagacgacc
     3841 gctcgtcgcg caaccgccgg tattgcgcgt caatcggtaa acacctctgg cgggttaagc
     3901 ctccataaaa gcccgaacgt ccttaacaga ctgataacga atcgcgcgcc gcgtaagatt
     3961 ttggaaggga taatgtcgcc gccgacataa cgccgtcata ttatggcgtg tcgcgataac
     4021 ggcagaccgg ttcgcgcatg tggtcgcgga ccagaggccc ggcggtcgag aactcgatat
     4081 agcgccgggc ggcgccgaag gacggccagc cggcaaggcc cgcgccgttc ggatcgccgg
     4141 tttggcgaat tgcgtccagt aatccagcag gcgcggccat tctgcatccg tcctgcccgc
     4201 cggttgcggg ttcatcacga aggcgaggtc ggaggaatga tgcggcggcc cggcgccgtc
     4261 attgacgtcg agttgatagg accagacgga cggacccgcc tgcgtctgga cctgcgcgac
     4321 ccattgcgac gggcagcgca tcatcaggtc ggtggatagc tgcatggcga cgtcgccgta
     4381 cagcggatcg gcgggcggtt ccgtcgtcgc ggtcacgccg taaaaggcca tcgccccggc
     4441 ggcgtgggcg ccgaactggc gtacgatttc ggcatgcacg gcgtccggcc cgccgaacag
     4501 gcccagctcc tgcgccaccg tgccgatgat cagcggaacc ttcgcctgct cgcccttgcg
     4561 gaaggcgtcc cgcggcgact gcgtcagcac cttgccgtcg atcaccgcct gcaaccagat
     4621 gaagccgtcg tccgcgatcg gaaccggcag ccggtcggcg gcgaactgca accgccgcgc
     4681 cggaacctcg cgcagcgcgg ccagcgccgc gtcgctgtcg ggaacagcgt cggtgtagaa
     4741 ccgcgccaga tcgacgccca tcgcctcgtt ctgcgccagc gtgcgcgcgg gcaggccgaa
     4801 ctgcggcggg ccgctttcct cgatagcttt ctgaaacagg ccgcgcgcgg cgggcgcggc
     4861 cagcagcatg ccgacatcct cggcgcccgc gctctggccg aagatcgtga cattgcccgg
     4921 atcgccgccg aactgcgcga tgttgtcgcg cacccaggca agcgccttga tctggtccat
     4981 cagcgcgtaa ttgccgctgt gcccgccctg ctcgcggctt aagcccggat gcgacaggaa
     5041 gccgaacacg ccgaggcggt attgcagcga gacgatgatc acgcccttgc ccgcgaagcc
     5101 cgccatcacg gtccccgcgc cgccgcccgc gcgattggcg ccgccgtgga tatagaccat
     5161 gaccggcaaa ggcttttgcg gatgcaggtc cggcgtcctg acctccagat agaggcagtc
     5221 ctccgacgac ttcgccgcca tgtcggtctg ccagccatag tcgatctgca agcaggcggg
     5281 cgcgctgtcc gtctcatggc gcacgccggt ccacggaacg acgtcctgcg gcgcgcgcca
     5341 gcgcagatcg cccagcggcg gcgccgcgaa cggcacggcg tgaaaaacat aggaattgtc
     5401 agccagccgg tccccggcca gcgctccgcc cgtgacgcgc agcgtgacgt ccgccgcctg
     5461 cgcgcccata gccgccaacg ccagaacggc gctcagcgcc catccggcaa gccgcctcac
     5521 ggatggacct cggctttcag gtgctggccc tgcgcccaca gataggcggc gccgcgcacg
     5581 ccggaggaat cgccccatct ggccgggacg atctcggcct cccacacgtc ggaaaagaca
     5641 tagggccgga tcttgcccgc cagccgctca tacagctccg gcgcgttcga cagcccgccg
     5701 cccagcacga acacgtcggg atcgaccata ttgaccagca cggccatgcc gcgccccagc
     5761 cggtcgacca gccggtcgaa agccgcctcg gcctcggcgt cccccgcgcg catggcggcg
     5821 atgatctcct cgcctttcag cgtgttgccg gtcaccgcat ggaagtcgcg cgcaaagccc
     5881 gtgccggaca cccaggtttc gaggcagccg tgcaggccgc accagcattt ggcgccggga
     5941 tattcctccg gcttcggcca cggcagcggc atatgccccc attcgcccgc cacgccgttg
     6001 gcgccctcga tcagcttgtg atcgatgacc agcccgccgc cgcagccggt gccgacgatg
     6061 atggcgaaga cgttggagcg cccccggccc gcgccgtcga tggcctcgct cagcgccagg
     6121 cagttggcgt cgttggccag ccgcaccggg cgttgcagcg cggcttcgag atcttcgcga
     6181 aacgtcttgc cgttcagcca cgtcgaattg gcgttgcgca tcacgccggt gcgcggcgac
     6241 acgctgcccg gcgcgccgac gccgatggtg gccacgcgtc cgctcaggga cggcgccctg
     6301 gcgtgggcct c
//
LOCUS       DASVLL010000622         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_528713,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000622 DASVLL010000000
VERSION     DASVLL010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528713"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VF402_10245"
     CDS             <1..436
                     /locus_tag="VF402_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HEX7800721.1"
                     /translation="NRKPSSRRGYIDDVTTPLYPFGYGLSYTRFEISAPRLTKDRIAT
                     NGTTQVQVDVQNTGAVRGDEVVQMYIRDDVSSVTRPLLELKGFQRVTLEPGEKRTVTF
                     DIKPTDLWFYNGDMKRVVEPGTFTIFAGPNSVDLKKATLTVA"
     gene            452..1636
                     /locus_tag="VF402_10250"
     CDS             452..1636
                     /locus_tag="VF402_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7800722.1"
                     /translation="MTAIQANPLPQDLDQALMVGRVWRTAPEGKGTEGPAVVAVRNGR
                     VIDITRHAPTVSELLERDDLVAIVRGADGEDLGDVNALVAAALAGDEGNAVRLLAPCD
                     LQAIKACGVTFAVSLLERVIEEQAKGDPARAAALRQQLQQTIGTNLSELEPGGEAAMR
                     LKADLSARGMWSQYMEVGIGPDAEVFTKSQPMSAVGHGAAVGLHPSSHWNNPEPEIVL
                     AVNSRAQVVGATLGNDVNLRDIEGRSALLLGKAKDNNGSCAIGPFIRLFDGDFDIDTL
                     RNAEVRMLIEGADDDFELAGASFMREISRDPLDLVRQTCGKHHQYPDGFMLFLGTMFS
                     PIKDRDAQGGGFTHHAGDRVSISTPSLGTLVNTVQRSDAIAPWTFGVRALYANLAQRG
                     LL"
     gene            1658..>1855
                     /locus_tag="VF402_10255"
     CDS             1658..>1855
                     /locus_tag="VF402_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7800723.1"
                     /translation="MSEQNQAFVYATYPDLAGKRVVITGGGSGIGGELTVAFARQGAR
                     VWFLDIAEDASRALEASLANSP"
BASE COUNT          358 a          648 c          593 g          256 t
ORIGIN      
        1 caaccgcaag ccgagcagcc gccgcggcta catcgacgac gtgaccacgc cgctgtaccc
       61 gttcggctat ggcctgtcgt acacccgctt cgagatctcc gcgccgcgcc tcacgaagga
      121 ccggatcgcg accaacggta ccacccaagt acaagtcgac gtgcagaaca cgggcgccgt
      181 acgtggcgac gaagtcgtgc agatgtatat ccgcgacgac gtcagctccg tcacccgtcc
      241 gctgctggaa ctgaagggct tccagcgcgt gacgctggag ccgggcgaaa aacgcacggt
      301 caccttcgac atcaagccga ccgacctgtg gttctacaac ggcgacatga aacgcgtcgt
      361 ggagccgggg acgttcacca ttttcgcagg accgaacagc gtcgacctga agaaggcgac
      421 cctgacggtg gcataaccaa gagagacaaa catgacagca atccaagcca atccgctgcc
      481 gcaggacctc gaccaggctc tgatggtcgg ccgcgtctgg cgcaccgctc ccgaaggaaa
      541 aggaactgag ggcccggccg tagtggccgt ccgtaacggt cgtgtaatcg atatcacgcg
      601 gcacgccccg accgtgtcgg aactgctcga gcgcgacgac ctggtcgcca tcgtgcgcgg
      661 cgcggacggc gaggacctgg gcgacgtcaa cgcgctggtc gccgccgcgc tggccggcga
      721 cgaaggcaac gcggtgcgcc tgctggcccc gtgcgacctg caggcgatca aggcctgcgg
      781 cgtcaccttc gccgtcagcc tgctggaacg cgtgatcgaa gagcaggcca agggcgaccc
      841 ggcccgcgcc gccgcgctgc gccagcagct gcagcaaacc atcggcacga acctgtcgga
      901 actggaaccg ggcggcgagg ccgcgatgcg cctgaaggcc gacctgtcgg cgcgcggcat
      961 gtggtcgcaa tacatggaag tgggcatcgg cccggacgcc gaagtgttca ccaagtcgca
     1021 gccgatgtcg gcggtcggcc acggcgccgc ggtcggcctg cacccgtcgt cgcactggaa
     1081 caatccggaa ccggaaatcg tcctcgccgt gaacagccgc gcgcaagtcg tgggcgccac
     1141 gctgggcaac gacgtcaacc tgcgcgacat cgaaggccgc agcgcgctgc tgctgggtaa
     1201 ggcgaaggac aacaacggct cgtgcgccat cggcccgttc atccgcctgt tcgacggcga
     1261 tttcgacatc gatacgctgc gcaacgcgga agtgcgcatg ctgatcgaag gcgccgacga
     1321 cgatttcgaa ctcgcgggcg ccagcttcat gcgcgagatc agccgcgacc cgctggacct
     1381 cgtgcgccag acctgcggca agcaccacca gtacccggac ggcttcatgc tcttcctggg
     1441 cacgatgttc tcgccgatca aggaccgcga cgcgcaaggc ggcggcttca cccaccacgc
     1501 gggcgaccgc gtcagcatct ccacgccgtc gctcggcacg ctggtcaaca ccgtccagcg
     1561 cagcgatgcg atcgcaccgt ggaccttcgg cgtgcgcgcg ctgtacgcca acctggccca
     1621 acgcggcctg ctgtgaaccg aacataacga gaattacatg tctgaacaaa accaagcatt
     1681 cgtctacgcg acgtacccgg acctcgccgg caagcgggtc gtgatcaccg gcggcggcag
     1741 cggcatcggc ggcgaactga ccgtcgcctt cgcccgccaa ggcgcccgcg tctggttcct
     1801 cgacatcgcc gaggacgcgt cgcgcgcgct cgaagcgtcg ctggcgaact cgccg
//
LOCUS       DASVLL010000623         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_259198,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000623 DASVLL010000000
VERSION     DASVLL010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259198"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VF402_10260"
     CDS             <1..803
                     /locus_tag="VF402_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7800724.1"
                     /translation="LMIDGCDGISFTGSVPTGTRVRDQAVAKGKRVQCEMGGKNPVVV
                     LDDADLNIAVNSVLNSSFFSTGHRCTASSRIIVTEGIADKFVSELAKATQAIKVGDSR
                     AAGVQMGPIASQEQLDIARKAVAKAKEQGGAVVAGGEDLTFGTPGYYYAPTLIDKTTP
                     DMEINREEVFGPVASVIRVKDLEEAIKVANDTEMGLSAGICTTSLKSAETFKRRIQSG
                     MVMVNLPTAGVDYHVPFGGRKASSFGPREQGAYAVEFYTIVKTAYIQP"
     gene            968..1789
                     /locus_tag="VF402_10265"
     CDS             968..1789
                     /locus_tag="VF402_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family lipase"
                     /protein_id="HEX7800725.1"
                     /translation="MSFRAFALAAALAFSAGAANAQDLKPIRIILAGDSTMAHKSGYG
                     DAFCAQFRPQVTCLNFGRGGRSSKSYRVDGIWDQSVMAELKKTANAEGVPYARTYVLM
                     QFGHNDGSPKPERHTDVLWEYPANLWGFAQDAQKAGAEFVLVTPISMRDFVDNRLLPD
                     RVAPFAASMQNVAIEHHLPLIDLFGESQRAVQAMGNVAANHYLAGQPVPQNVLDSEKT
                     GTSVPAKFTDASQGQAETQPFDYTHMNQTTGATFFGRMVANLLAQTEPDTIPYLK"
     gene            complement(1782..2252)
                     /gene="msrB"
                     /locus_tag="VF402_10270"
     CDS             complement(1782..2252)
                     /gene="msrB"
                     /locus_tag="VF402_10270"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7800726.1"
                     /translation="MRKSVLGFDLTPPSPEQIRALAARLDPEARRILLDHGTEPPFCG
                     AFEDHEEEGTYICNLCALPLFSSRAKFHSGSGWPSFYEDLGEGHVRYLRDASHGMTRT
                     EIRCGRCDSHLGHVFEDGPRPTGLRYCMNSVAMDFVPEGQPLPDRLGRGDPASI"
     gene            complement(2265..2834)
                     /locus_tag="VF402_10275"
     CDS             complement(2265..2834)
                     /locus_tag="VF402_10275"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7800727.1"
                     /translation="MYDFLSYRPVSEDDAELLLDWRTAPHITQYMLSDVPYDIERQKA
                     WIARSNARDDYRHAIIRIEGRDVGYVSITVTDARNGVGEIGCYIGDETAPKALTAYNF
                     VGTLNHAFFTLGLYKLVNHIIAWNARTVRAQGFNGYRHVGVLKDHVSKDGARHDLHIF
                     EQSAAEWAEFRKKFRDDRNWRGEATVYKG"
     gene            complement(2842..>3554)
                     /locus_tag="VF402_10280"
     CDS             complement(2842..>3554)
                     /locus_tag="VF402_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800728.1"
                     /translation="SYAADEAYIAALSLMTRVCVIDDLNLLSAYDCDLLVNAAGAAPH
                     MGYRSKTRAKCLLGAEYALIRREFREAGPRGGETVTLMFGGSDPQNFSAQAARLLLAQ
                     GLRVRILAGPANRHIAALRALANDRLEVAVSPPSVAGTLADSALIVTAAGGSVGEVAV
                     MGLPALVLVVYDNQAAALAACPYPVLDARGGLPDDLGARIKTLLADPAGLRANAGRAH
                     AKVDGWGPKRIIEALING"
BASE COUNT          649 a         1201 c         1127 g          577 t
ORIGIN      
        1 cgctgatgat cgacggctgc gacggcatct ccttcaccgg ctcggtgccg accggcacgc
       61 gcgtgcgcga ccaggccgtc gccaagggca agcgcgttca gtgcgaaatg ggcggcaaga
      121 acccggtcgt ggtgctggac gacgccgacc tcaacatcgc cgtcaattcg gtgctgaact
      181 cgtccttctt ctcgaccggc caccgctgca cggcctcgtc gcgcatcatc gtcaccgagg
      241 gcatcgcaga caagttcgtc tccgaactgg ccaaggcgac ccaggcgatc aaggtcggcg
      301 attcgcgcgc ggcgggcgta cagatgggcc ccatcgcctc gcaggaacag ctcgacatcg
      361 cgcgcaaggc tgttgcgaaa gcgaaagaac agggcggcgc cgtcgtcgcg ggcggcgagg
      421 atctgacctt cggcacgccg ggctattact acgccccgac cctgatcgac aagacgaccc
      481 cggacatgga gatcaacagg gaggaggtct tcggaccggt cgcctcggtc atccgcgtca
      541 aggatctcga agaagcgatc aaggtcgcca acgacaccga aatgggcctg tcggcgggca
      601 tctgcaccac ctcgctgaag tcggcggaaa ccttcaagcg ccgcatccag tcgggcatgg
      661 tcatggtcaa cctgccgacc gccggcgtcg actaccacgt cccgttcggc ggccgcaaag
      721 cctccagctt cgggccacgc gaacaaggcg cctacgcggt cgaattctac accatcgtca
      781 agacggccta tatccagccg taagacgcca tcgacgtcgc tgagcagaaa cggcgcttcc
      841 tgaacgggaa gcgccgtttc tttttgcaat gctctaaaat gatcaaattt gctcttaact
      901 gacgtttttg cttgcgccga acgcgccgca ggccctttat gcctccacac agatcaagga
      961 ggcccccatg tcgttccgcg cattcgccct cgccgccgcg ctcgcgtttt ccgccggcgc
     1021 cgccaacgcc caagacctca agcccatccg catcattctg gcgggcgact cgaccatggc
     1081 gcacaagagc ggctatggcg acgccttctg cgcgcagttc cgcccgcagg tcacgtgcct
     1141 gaacttcggc cgcggcgggc gcagcagcaa gagctaccgc gtcgacggca tctgggacca
     1201 gagcgtgatg gccgagctga aaaagaccgc caacgccgag ggcgtccctt acgccaggac
     1261 ctatgtgctg atgcagttcg gccataatga cggctcgccc aagccggagc gccacacgga
     1321 cgttttatgg gaatacccgg ccaatctgtg gggctttgcc caggacgcgc agaaggccgg
     1381 cgccgaattc gtgctggtca cgccgatctc gatgcgcgat ttcgtcgaca accggctgct
     1441 gcccgaccgc gtggcgccgt tcgccgcctc gatgcagaac gtcgccatcg aacatcatct
     1501 gccgctgatc gacctgttcg gcgaaagcca gcgcgcggtg caggcgatgg gcaatgtcgc
     1561 cgccaaccac tatctcgccg gtcagccggt cccgcaaaac gtgctggaca gcgaaaagac
     1621 cggcacgtcg gtcccggcca aattcaccga cgcctcgcag ggtcaggccg agacccagcc
     1681 cttcgactac acgcacatga accagaccac cggcgcgacc ttcttcggcc gcatggtcgc
     1741 caacctgctg gcccagaccg agccggacac gattccgtac ctcaaataga ggccggatcg
     1801 ccgcgcccca gccggtccgg caaaggctga ccttcgggaa cgaaatccat cgccaccgaa
     1861 ttcatgcagt agcgcaggcc ggtcggacgc ggcccgtcct cgaacacgtg gccgaggtgc
     1921 gaatcgcagc gtccgcagcg gatttcggtg cgcgtcatgc cgtgcgacgc gtcgcgcagg
     1981 taacggacgt gcccctcgcc gagatcttca taaaagctgg gccagcccga accggaatgg
     2041 aacttggcgc gcgaggaaaa caaaggcagg gcgcacaggt tgcagatata ggttccctcc
     2101 tcctcatggt cttcgaacgc gccgcagaac ggcggctcgg tgccgtggtc gagcaggatg
     2161 cggcgcgctt ccgggtcgag gcgcgcggcc agagcccgga tttgctccgg cgagggcggg
     2221 gtcaggtcga aaccgaggac ggacttgcgc atgggatatt cctttcagcc cttatatact
     2281 gtcgcctcgc cccgccagtt acggtcatcg cgaaactttt tgcggaattc cgcccattcc
     2341 gccgccgact gctcgaaaat atgcaggtcg tggcgcgcgc cgtccttcga cacatggtcc
     2401 ttcagcacgc cgacatggcg gtagccgttg aagccctgcg cccgcacggt gcgcgcgttc
     2461 caggcgatga tgtggttgac cagcttgtag aggcccagcg tgaagaaggc gtggttgagc
     2521 gtgccgacga aattgtaggc ggtcagcgcc ttgggcgcgg tttcatcgcc gatatagcag
     2581 ccgatctcgc cgacgccgtt acgcgcgtcg gtgaccgtga tcgagacgta gccgacgtcg
     2641 cgcccctcga tgcggataat ggcgtgccgg tagtcgtcgc gcgcgttgga gcgcgcgatc
     2701 cacgcctttt gccgttcgat gtcgtaaggc acgtcgctca gcatgtactg cgtgatgtgc
     2761 ggcgcggtgc gccagtcgag cagcaattcg gcgtcgtctt cgctcaccgg gcggtaggac
     2821 aggaagtcat acatggcgca tttatccgtt tatcagggcc tcgattatgc gtttcggtcc
     2881 ccaaccgtcg accttcgcat gggcgcggcc ggcgttggcg cgcaggcccg ccggatcggc
     2941 cagcagggtt ttgatccgcg cgccgagatc gtccggcagg ccgccgcgcg cgtcgagaac
     3001 cggatagggg caggccgcca gcgccgccgc ctgattgtca tagacgacca gaaccagcgc
     3061 gggcaggccc atgaccgcga cctcgccgac gctgccgccc gccgccgtga cgatcagggc
     3121 gctgtcagcc agagtcccgg ccacggaggg cggcgagacg gcgacctcca gacggtcgtt
     3181 cgccagcgcg cgcaaggccg cgatatgacg gttggccggc ccggcaagga tgcggacgcg
     3241 caggccctgc gccagcagca agcgcgccgc ctgcgcgctg aaattctgcg gatcggagcc
     3301 gccgaacatc agcgtcaccg tctcgccgcc gcgcggcccg gcctcacgga actcgcgccg
     3361 gatcagcgcg tattccgcgc cgagcaggca tttcgcgcgc gtcttgctcc ggtagcccat
     3421 gtgcggcgcg gccccggcgg cgttgacgag caggtcgcag tcgtaagccg aaagcaggtt
     3481 cagatcgtcg atcacgcaca cccgcgtcat caggctcagg gcggcgatat aggcctcgtc
     3541 cgccgcatag gaat
//
LOCUS       DASVLL010000624         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_419339,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000624 DASVLL010000000
VERSION     DASVLL010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419339"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..845
                     /locus_tag="VF402_10285"
     CDS             24..845
                     /locus_tag="VF402_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX7800729.1"
                     /translation="MIRSLFAAASVLALSAGAAQADTEVSLKNIAARVVVIPENRSDI
                     DLRVVYGKAKVPVVMVHDEGSKLVADGKLGRRGVGCKADGADVPGVGFVALADLPTVY
                     IRTPPDAKVTTGGAVWGEVRASRSLELGVGGCGKWTLGDVANKAEVSIGGSGDVTAGR
                     MGDSEINIGGSGNFTGGAAQALEANIGGNGDVSLSRVDGPVEVNIGGSGNVTVGEGQS
                     PSLEVNIAGSGDVRHNGAVGDLAVNIVGSGDVRVHAVTGRVSRSIMGSGKVIIGQ"
     gene            928..>1979
                     /locus_tag="VF402_10290"
     CDS             928..>1979
                     /locus_tag="VF402_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX7800730.1"
                     /translation="MFEEEVRRRIRFTPAPGWVREQPFVRPARLDAALAPWGFYTWLM
                     DDQARLDRRPQRYNRTIEEIVNLSALQELANISVPFNPYHDDLQIHHVRILRGDQVIE
                     VDVETRFLVLRRERAFERLVLDGRWTLTVTLPDVRVGDLIDMAMTLSGDPACFQGEFT
                     VPAPLQNVVYWDRRHVRLLVPPDRRIFLQPFPAGGAQPAVAALDDGYSEIRFDLTHVP
                     ACDYEPDMPGWVLPARGVFASSTDSWERVNDVMRAGFEGDEDYPDGFLQVIAEIEAAH
                     ADTADRIVAAVRWVQENIRYFAFSFGEGGFTPRPLRDIFADRIGDCKDVSKLICAMLT
                     KMGVEAWPALVDTRRG"
BASE COUNT          300 a          623 c          719 g          337 t
ORIGIN      
        1 cacagctttc acaggatgcc gatatgatca ggagtctttt cgccgccgcc tccgttctgg
       61 cgctgagcgc cggggcggcg caggccgata ccgaagtcag cctcaagaac atcgccgcgc
      121 gcgtcgtggt gatcccggag aaccgttcgg acatcgacct gcgcgtcgtg tacggcaagg
      181 cgaaagtccc ggtggtcatg gtgcatgacg aaggaagcaa gctggtcgcc gacggcaagc
      241 tgggccgccg cggcgtcggc tgcaaggcgg acggcgccga tgttccgggc gtcggcttcg
      301 tggcgctggc cgatctgccg accgtctata tccgcacgcc gccggacgcg aaggtgacga
      361 cgggcggggc cgtgtggggc gaggtccgcg ccagccggtc gctggagctg ggcgtgggcg
      421 gttgcggcaa gtggacgctg ggcgacgtcg cgaacaaggc ggaggtcagc atcggcggat
      481 cgggcgacgt gacggcgggc cgcatgggcg attccgaaat caatatcggc ggctccggga
      541 acttcaccgg cggcgcggcg caagcgctcg aggccaatat cggcggcaac ggcgacgtgt
      601 cgctgagccg cgtcgacggg ccggtcgagg tcaatatcgg cggttccggc aatgtcacgg
      661 tcggcgaggg ccagtcgccc agtctggagg tcaacatcgc cggttcgggc gacgtgcgtc
      721 ataacggcgc ggtgggcgac ctcgcggtca atatcgtcgg ctccggcgat gtgcgcgtcc
      781 acgccgtcac cggcagggtg tcgcgctcga tcatggggtc gggcaaggtg atcatcgggc
      841 aataattcgc ccgatgcgca atcgtccttt tgcgcgcccc gcgcctgtgt taaccctgac
      901 gccgaggctg cggacagggg gagaagggtg ttcgaggagg aggtcaggcg gcgaatccgg
      961 ttcacgcccg cgcccggctg ggtgcgcgag cagccgttcg tccgcccggc ccggctcgac
     1021 gccgcgctgg cgccgtgggg gttctacacc tggctgatgg acgatcaggc gcggctggac
     1081 cggcgcccgc agcgctataa ccgcaccatc gaggagatcg tcaacctgtc ggccttgcag
     1141 gagctggcca atatcagcgt gccgttcaac ccgtatcacg acgatcttca gattcatcat
     1201 gtccgtatcc tgcgcggcga tcaggtcatc gaggtcgatg tcgagacacg ctttctggtg
     1261 ctgcggcgcg agcgcgcgtt cgagcggctg gtgctggacg ggcgctggac gctgaccgtg
     1321 accctgccgg acgtgcgcgt cggcgacctg atcgacatgg cgatgacgct gagcggcgac
     1381 ccggcctgtt ttcagggcga attcaccgtg ccggcgccgt tgcagaacgt cgtctattgg
     1441 gaccgtcgcc atgtccgcct gctggttccg ccggaccggc ggatcttcct gcaaccgttt
     1501 ccggcgggcg gggcgcagcc cgccgtcgcc gcgctggacg acggctacag cgagatccgt
     1561 ttcgacctga cccatgttcc cgcctgcgat tacgagccgg acatgccggg ctgggtcttg
     1621 cccgcgcgcg gcgtcttcgc ctcgtcgacc gatagctggg agcgcgtcaa cgacgtgatg
     1681 cgcgcgggct tcgagggcga cgaagattat cccgatggct ttttgcaagt catcgcggag
     1741 atcgaggcgg cgcacgccga tacggcggac cggatcgtcg cggcggtgcg ctgggttcag
     1801 gaaaacatcc gctatttcgc cttttcgttc ggcgagggcg ggttcacgcc gcggcccctg
     1861 cgcgacatct tcgccgaccg catcggcgac tgcaaggacg tgtcgaaact gatctgcgcc
     1921 atgctgacga agatgggcgt cgaggcgtgg cccgccctgg tcgatacgcg gcgcggctt
//
LOCUS       DASVLL010000625         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_204169,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000625 DASVLL010000000
VERSION     DASVLL010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204169"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /gene="pbpC"
                     /locus_tag="VF402_10295"
     CDS             complement(<1..1711)
                     /gene="pbpC"
                     /locus_tag="VF402_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX7800731.1"
                     /translation="MALEVLLCVLNLAFPPDMTKAMRASPVVLDRNGVFLRALPVDDG
                     RWRIRADLDGADPVFLHNLLGVEDERFYWHPGVDATAIARAFLSNLHAGGIVSGGSTI
                     TMQTARLLSPHPRSYGNKAIEALRALQLELRYSKRDILQLYLTLAPYGGNLEGVRAAS
                     LSYFGHEPDSLTLGEQALLICLPQAPEARRPDLHPKAAQAARDLILRRMQARNLIGAQ
                     AMNEAMQEPAAHSRLPFPTLAWHLAGRLARHVRSSKIWGLQATIHTTIDARLQTRLEA
                     MAAATAKAQGPNVSAAVMVVDIRTRGVLASVGGAGLDRPGGWLDMTQALRSPGSSLKP
                     FIYGFAFENGVVAPDTRLMDAPTRFGDYQPEDFDRSFHGEVSVKEALINSLNVPAVAV
                     LNRIGPDVFQARLAAVGVRLVRPKSSLHDAGLALALGGAGIKLADLAELYAGLGDHGL
                     VKPLAYELKDEQSNLKASGKRLMRAEAADKVIGILRETPPPPGRLPGPLMRVANRPAY
                     KTGTSYGYRDALAVGVAGGYAVMVWTGRPDGGARADATGRDSAGPLLFDTFDLLRAPS
                     QAPQ"
BASE COUNT          309 a          650 c          707 g          291 t
ORIGIN      
        1 cctgcggcgc ctgcgacggg gcgcgcagca ggtcgaacgt atcgaacagc aaaggcccgg
       61 cggagtcgcg gcccgtcgcg tcggcgcgcg cgccgccgtc ggggcgaccc gtccagacca
      121 tgaccgcata gccgcccgcc acgccgaccg ccaaggcgtc gcgatagccg taggaggtgc
      181 cggtcttgta ggccgggcgg ttggcgacgc gcatcaaagg ccccggcaaa cgtccgggcg
      241 gcggcggggt ttcgcgtaag attccgatga ccttgtcggc ggcctcggcg cgcatcagcc
      301 gtttgccgga cgctttcaga ttggactgtt cgtccttcag ttcgtaggcc agcggcttga
      361 cgaggccgtg atcgccgagg cccgcgtaaa gctcggccag atcggccagc ttgatgcccg
      421 ccccgcccag cgccagagcc agccctgcgt cgtgcaggct cgatttgggc cgcaccagcc
      481 tgaccccgac cgccgccagc cgcgcctgaa acacgtccgg cccgatgcgg ttcagcacag
      541 cgacggcggg cacgttgagc gaattgatca gcgcctcctt caccgacacc tcgccgtgga
      601 aactgcggtc gaaatcctcc ggctgatagt cgccgaagcg cgtcggcgcg tccatcaggc
      661 gggtgtccgg cgcgacgacg ccgttctcga acgcgaagcc gtagatgaag ggcttgagcg
      721 acgaaccggg cgagcgcagg gcctgcgtca tatccagcca gccgccgggc cggtccagtc
      781 ccgccccgcc gacgctggcc agcacgccgc gcgtgcggat atcgaccacc atcaccgccg
      841 ccgacacgtt cggcccctgc gccttggcgg tggcggcggc catcgcttcc agccgggttt
      901 gcaggcgcgc gtcgatggtg gtgtggatgg tggcctgaag accccaaatc ttagagcttc
      961 tgacgtggcg cgccagccgt ccggccagat gccaggccag cgtcgggaac ggcaggcggc
     1021 tgtgggcggc gggctcctgc atggcctcgt tcatcgcctg cgcgccgatc aggttccgcg
     1081 cctgcatccg gcgcaggatc aggtcgcgcg ccgcttgcgc cgccttggga tgcaggtcgg
     1141 gacggcgcgc ttcgggcgcc tgcggcaggc agatcagcaa cgcctgttcg ccgagggtca
     1201 ggctgtccgg ctcatggccg aaatagctga gcgaggcggc gcgcacccct tcgagattgc
     1261 cgccataagg ggccagggtc agatagagtt gcaggatatc gcgcttggaa tagcgcagct
     1321 ccagttgcag agcgcgcagg gcctcgatgg ccttgttccc gtaggagcgc ggatgcgggc
     1381 tgagcagccg cgcggtctgc atggtgatgg tcgagccgcc ggacacgatg ccgcccgcgt
     1441 gcaggttgga caggaaggcg cgcgcgatcg ccgtcgcatc gacgccgggg tgccagtaga
     1501 agcgttcgtc ctcgacgccg agcaggttgt gcagaaagac cggatcggcg ccgtcgagat
     1561 cggcgcggat gcgccagcgt ccgtcatcga cgggcaaggc gcgcagaaag acgccgttgc
     1621 ggtccagcac caccggcgag gcgcgcatgg ccttggtcat atcgggcgga aaggcgaggt
     1681 tgaggacgca gagcaggact tcgagcgcca tcagggcgat gagcagcttg accagcgggc
     1741 ggatgagggc gtcggcctta agcttgggca aggcgcgtca ccttcccctc tccccgttga
     1801 cggggagagg acgagcgccg aacgtagtga aggcgatcgg gtgaggggct gttcgccgat
     1861 cgagcgcaac cgtcgggctg cccctcaccc gaggatcggc gacttcgcct tgcgcctcgt
     1921 cctctcccct cgtggagggg agagggggac tcagcag
//
LOCUS       DASVLL010000626         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_313210,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000626 DASVLL010000000
VERSION     DASVLL010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313210"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VF402_10300"
     CDS             complement(<1..1153)
                     /locus_tag="VF402_10300"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX7800732.1"
                     /translation="MTDILMPALSPTMEEGTLSKWLIKEGDEVSAGQVIAEIETDKAT
                     MEVEAVDEGKVEAILVPAGTENVKVNTPIARLEGGVSAPAPAPAAAAPEAKAEEAAPA
                     PAATAPVSKPKADPEFPEGTPMVKITVRDALRDAMAEEMRKDDKVFLMGEEVAQYQGA
                     YKVSRDLLQEFGDRRVIDTPITEMGFAGIGAGAAMAGLKPIVEFMTFNFAMQAIDQII
                     NSSAKTLYMSGGQIKSSIVYRGPNGAAARVAAQHSQDYSSWYAHVPGLKVIAPYDAAD
                     AKGLLKAAIRDPNPIVFLEHELMYGIEFEIPDVEDFVLPIGKAKIQRAGADVTITAHS
                     RMVGFALEAAEKLAAEGIDAEVINLRTLRPLDTDTIVESVRKTNRLVTVE"
     gene            complement(1160..>1756)
                     /locus_tag="VF402_10305"
     CDS             complement(1160..>1756)
                     /locus_tag="VF402_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7800733.1"
                     /translation="TGLAFADWYRNNGNVSFTYFGDGAANQGQVYESFNMAQLWKLPV
                     VYVIENNEYAMGTALARASATTELYQRGLSFGIPGEQVDGMDVFAVKAAAEKAAEHAR
                     SGKGPYILEMKTYRYRGHSMSDPAKYRSKEEVDEVKTKRDPIDHIKTMLQQAGVAEDE
                     IKALDNAVKAIVLEAVSFAQESPEPDPSELYTEVYLEA"
BASE COUNT          268 a          551 c          580 g          357 t
ORIGIN      
        1 cctcgaccgt caccagacgg ttggtcttgc gcaccgattc gacaatcgtg tcggtgtcga
       61 gcggacgcag ggtgcgcagg ttgatcacct cggcgtcgat gccctcggcg gccagctttt
      121 ccgccgcctc cagcgcgaag ccaaccatgc gcgaatgggc ggtgatggtc acgtccgcac
      181 cggcgcgctg gatcttcgcc ttgccgatcg gcagcacgaa gtcctcgacg tcgggaatct
      241 cgaactcgat gccgtacatc agctcgtgtt cgaggaagac gatcgggttc ggatcgcgga
      301 tggcggcttt cagcaggccc ttggcgtcgg cggcgtcata gggcgcgatc accttgaggc
      361 cgggcacatg ggcgtaccac gacgaatagt cctggctgtg ctgcgccgcg acccgcgcgg
      421 cggcgccgtt cgggccgcga tagacgatgg acgacttgat ctggccgccc gacatataga
      481 gcgtcttggc cgacgagttg atgatctggt cgatggcctg catggcgaag ttgaaggtca
      541 tgaactcgac gatgggtttc agacccgcca tggcggcgcc cgcgccgatg cccgcgaagc
      601 ccatttcggt gatcggggtg tcgatgacgc ggcggtcgcc gaattcctgc aacaggtcgc
      661 gcgacacctt gtaggcgccc tgatattgag cgacttcctc gcccatcagg aacaccttgt
      721 cgtccttgcg catctcctcg gccatcgcgt cgcgcagcgc gtcgcggacc gtgatcttca
      781 ccatcggcgt gccttcgggg aattccgggt cggctttcgg cttggaaacg ggcgccgtgg
      841 cggcaggcgc gggcgccgct tcctcggcct tggcctcggg cgcagcggcg gcaggcgccg
      901 gagcgggcgc ggacacgccg ccttccagac gcgcgatcgg cgtgttgacc ttgacgtttt
      961 cggttccggc ggggacgagg atggcctcga ccttgccttc gtcgacggct tcgacttcca
     1021 tcgtcgcctt gtcggtctcg atctcggcga tcacctgacc ggcggaaacc tcgtcgcctt
     1081 ccttgatcag ccacttgcta agggtgcctt cctccatggt cggggaaagc gcgggcataa
     1141 gaatatcggt catggaagat tacgcctcaa gatagacttc ggtgtagagt tcggacggat
     1201 cgggctcagg gctttcctgc gcgaagctga ccgcttccag cacgatggcc ttgaccgcgt
     1261 tgtcgagcgc cttgatctcg tcctcggcga cgcccgcctg ttgcagcatg gtcttgatgt
     1321 ggtcgatggg atcgcgcttg gtcttgacct cgtcgacctc ctccttggag cggtacttgg
     1381 ccgggtcgga catcgagtgg ccgcgatagc ggtaggtctt catttccagg atgtacggcc
     1441 ccttgccgga gcgcgcgtgt tcggcggcct tttcggcggc ggccttgacg gcgaacacgt
     1501 ccatgccgtc cacctgctcg cccggaatac cgaagctgag gccgcgctga taaagctcgg
     1561 tcgtcgccga ggcgcgggcc agggccgtgc ccatggcgta ttcgttgttc tcgatcacgt
     1621 acacgaccgg caacttccac aactgggcca tgttgaagct ttcatagacc tggccctgat
     1681 tggccgcgcc gtcgccgaaa taggtgaagg agacgttgcc gttgttgcgg taccagtcgg
     1741 cgaaggccag acccgt
//
LOCUS       DASVLL010000627         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_528822,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000627 DASVLL010000000
VERSION     DASVLL010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528822"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VF402_10310"
     CDS             complement(<1..1412)
                     /locus_tag="VF402_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7800734.1"
                     /translation="MTSTPIPNLFDGGEPEAPKPSAQPAAKPEARKPAEVVPAPVRPQ
                     PAVDAGYNASSIEVLEGLEPVRKRPGMYIGGTDEKALHHLFAEVLDNAMDEAVANHAK
                     AIWVELDADGYVSVRDDGRGMPVDPHPKYPGKSALEVIMTVLHSGGKFSGKAYATSGG
                     LHGVGASVVNALSERLEVTVWRDGHEWRQNFSRGHALAPIEQVQPSKKRGTKLRFAPD
                     AEIFGSHHFKPARLYRMTKAKAYLFRGVQIFWKCAADVIHDQTPAEDKFYFPNGLADY
                     LAERVKDTETVSDIFSGRVERADDAGAVEWAVVWSAAGFGEHDGFVQSYCNTIPTPDG
                     GTHEAGLRAALTRSLKAYAEMTGDKRGGLITAEDVAASAGVVISVFIRNPEFQGQTKD
                     RLSSPDAARLVERALADPFDHWLTSSPKQADKLLQFIVEKAEERLKKRKDKEIQRASA
                     TRKLRLPGKLADCARQDAVGT"
     gene            complement(1557..2255)
                     /locus_tag="VF402_10315"
     CDS             complement(1557..2255)
                     /locus_tag="VF402_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX7800735.1"
                     /translation="MKKTLLVAAVALSALFAGSAAMAQDNAAGSLTFNIAATSNYVWR
                     GVTQTNNGAAVQGGADYSKGIFYAGTWLSNVDWGTSTNTEADFYMGVTPKVGNYGFDF
                     GVIYYTYPGHDDADKGSVGELKAAVNHTMGKGTIGAAMYLPTESLEDPYYEVNASYPL
                     TDKLTLSGAVGNYESGAYSYTDGAGQLINATGYTTENIGVTYALTQKLGLDLRWTNAS
                     RLPSTFAATLKATF"
BASE COUNT          359 a          728 c          804 g          455 t
ORIGIN      
        1 gtgccgaccg cgtcctgacg cgcgcagtcg gccagcttgc ccggcaggcg cagcttgcgc
       61 gtggccgagg cgcgctggat ttccttgtcc ttgcgcttct tgagccgttc ctcggccttt
      121 tcgacgatga attgcagcag cttgtcggcc tgtttcggcg acgaggtcag ccagtgatcg
      181 aacggatcgg ccagcgcccg ctcgaccaga cgcgccgcgt cgggcgagga caggcggtcc
      241 ttggtctggc cctgaaattc cgggttgcgg atgaagaccg agatcaccac gcccgcgctg
      301 gccgccacgt cctcggcggt gatcaggccg ccgcgcttgt cgccggtcat ttcggcgtag
      361 gccttcagcg agcgggtgag ggcggcgcgc aggcccgcct cgtgcgtgcc gccgtcgggc
      421 gtcgggatgg tgttgcagta ggactgcacg aagccgtcgt gctcgccgaa accggcggcg
      481 gaccagacca cggcccattc gaccgcgccc gcgtcgtcgg cgcgctcgac gcggcctgag
      541 aagatgtcgg agacggtttc ggtatccttg acgcgctcgg ccagatagtc ggccagaccg
      601 tttgggaagt agaacttatc ctccgccggc gtctggtcat gaatcacgtc cgctgcgcat
      661 ttccagaaga tctgcacgcc gcggaacagg taggctttcg ccttggtcat gcggtacagg
      721 cgcgcgggct tgaaatggtg cgagccgaag atttcggcgt cgggcgcgaa gcgcagcttg
      781 gtgccgcgct tcttcgacgg ctgcacctgc tcgatgggag ccagcgcatg gccgcgcgag
      841 aagttctgcc gccattcgtg gccgtcgcgc cagaccgtga cctccagccg ctcggacagg
      901 gcgttgacga ccgaggcgcc gacgccgtgc agcccgcccg acgtcgcata cgccttgccg
      961 gagaatttgc cgcccgaatg cagcacggtc atgatgacct cgagcgcgga cttgcccgga
     1021 tatttcgggt gcggatcgac cggcatgccg cgcccgtcgt cgcgcacgct gacatagccg
     1081 tcggcgtcga gttcgaccca gatcgccttg gcgtggttgg cgacggcttc gtccatcgcg
     1141 ttgtccagca cttcggcgaa caggtggtgc agcgcctttt cgtcggtgcc gccgatatac
     1201 atgcccggac gcttgcgcac cggctccaga ccttccagca cctcgatgct ggatgcgttg
     1261 taacccgcat ccacggcggg ttgcggccgc acgggcgcgg gaacgacctc ggccggtttg
     1321 cgcgcttcgg gtttggcggc aggctgggcg gagggctttg gcgcttcggg ttcgccgccg
     1381 tcgaacaggt tggggatcgg agtgctcgtc atgtcctcag catcttatga ttcgcgccat
     1441 attcgtaaag cggacaacgc cgcgcaacag aaaaaggccg caggttcttc acgaacccgc
     1501 ggcctttgtg gaaggcgtta cgcctgaagc gtcgccggaa ggcttagccg gaaggcttag
     1561 aaggtcgcct tcagcgtcgc cgcgaaggtc gacggcagac gcgaagcgtt ggtccagcgc
     1621 aggtccaggc ccagcttttg cgtcagggcg taggtcacgc cgatgttctc ggtcgtgtag
     1681 ccggtggcgt tgatcagttg accggcgccg tcggtgtagc tgtaggcgcc gctttcgtag
     1741 ttgccgacgg cgcccgacag ggtcagcttg tcggtcagcg ggtacgaagc gttgacttcg
     1801 tagtacggat cttccagcga ctcggtcggc aggtacatgg ccgcgccgat cgtgcccttg
     1861 cccatcgtgt ggttgacggc ggccttcagc tcgccgaccg aacccttgtc ggcgtcgtca
     1921 tggccgggat aggtgtagta gatcacgccg aagtcgaagc cgtaattgcc gaccttcggg
     1981 gtgacgccca tatagaagtc ggcttcggtg ttggtgctgg tgccccagtc gacgttcgac
     2041 agccaggtgc cggcgtagaa gatgcccttg gaatagtccg cgccgccctg aacggccgcg
     2101 ccgttgttgg tctgcgtcac gccgcgccac acatagttgc tggtggcggc gatgttgaag
     2161 gtcagcgagc ccgcggcgtt gtcctgcgcc atggcggcgc tgccggcgaa cagggcgctg
     2221 agggccacgg cggcgacaag aagcgtcttt ttcatagtca ttttcccagt tcaatgatgt
     2281 cgccggtcgg acgggccgcc agcgattttg aggaacttat aaagttggtc ctgaaacctg
     2341 aggcta
//
LOCUS       DASVLL010000628         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_419461,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000628 DASVLL010000000
VERSION     DASVLL010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419461"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..891)
                     /locus_tag="VF402_10320"
     CDS             complement(139..891)
                     /locus_tag="VF402_10320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7800736.1"
                     /translation="MDTFTYDGAEPIRLNKWLAQSGVCSRREADALIGEGRVRVDGET
                     VTDAGRKLTRGQTVSFSDAQATAAKLTVIYNKPVGIVSGQPEPGETPAVRMIRRANVF
                     GPADTYPDHDSRLAPIGRLDKDSHGLLILSEDGVLAKAIIGPDSDLEKEYHVQVRGPL
                     FEEKLKWLRHGLELDGRQLKPAVVTQTGPQSLTFILKEGRNRQIRRMCDMCELRVVDL
                     LRVRIGSIALADLPEGQWRLMTPEERARLVRH"
     gene            1019..2713
                     /locus_tag="VF402_10325"
     CDS             1019..2713
                     /locus_tag="VF402_10325"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7800737.1"
                     /translation="MTKTEMMRAMKGLACLAAAIALPACAKAQEIAAGKAAFDADCSL
                     CHDASPARATFQGPPLFGVVGRKTGAVSGFAYSAALKSAGAKGQVWTAAALDRFLTDP
                     QKAMPGTAMPVSVADAKTRKAIIAYLSSLNGGKAKAAPKPVIKPVMKPAASASAAQDW
                     RADAPGVAHTVTAADLPAPYATQSAGNSAHYAAPDAGKLPVVPAGFAVSVFADGLDGP
                     RLLRAAPNGDLYAVASETGKVLVWRNAGGRLAAQPETFATGLRQPFGVAFYPLDKPRF
                     VYIANVDSIVRVPIGGGAAQTVVAHLSTAGGHHTRDIAFSPDGRYMYVSVGSGSNVAE
                     NMGAMPAGWIASHAPGEPWGSEAGRAMVLRFTPDGQDRHVVATGIRNCVSLAVRPGSD
                     ALYCVTNERDALGDNLVPDYFTQVKDGQFFGWPWWYLGDHQDPRHKGERGDLKGHVSL
                     PDTLFASHSAPLGLVFYRPAPGGAANFPADYTGDAFITLHGSWNRAQRTGSKLVRVRF
                     DHGRPSASYEDFMTGLIVDDHTVNGRPVGVTVGPDGALYVSDDAGGRIWRIAPVGK"
BASE COUNT          446 a          986 c         1047 g          477 t
ORIGIN      
        1 gccggccatc gccaggcgcg ccaaaagata ccgtccgaac atgatttccc cctgtgcggg
       61 ctgcgccgct taccgggagt ttaaacatgg acggcaggcg ctttcaagag aggccgcctg
      121 gcgggggaaa ccgcgcggct aatgcctgac caacctcgcc cgctcttccg gcgtcatcag
      181 ccgccattga ccttcgggca ggtcggccag ggcgatcgag ccgatgcgga cgcgcaggag
      241 gtccacgacg cgcagttcgc acatgtcgca catgcggcgg atctggcggt tgcggccttc
      301 tttcaggatg aaggtcaggg attgcgggcc ggtttgggtg acgacggcgg gcttgagttg
      361 gcggccgtcc agttcgaggc cgtggcgcag ccatttcagc ttttcctcga acagggggcc
      421 gcgcacctga acgtggtatt ccttctccag atcgctgtcc gggccgatga tggctttcgc
      481 cagcacgccg tcctcggaca ggatcaggag gccgtgcgaa tccttgtcga ggcggcctat
      541 gggcgccagt ctggagtcgt ggtcggggta ggtgtccgcc gggccgaaga cgttggcgcg
      601 gcggatcatg cgcacggcgg gcgtctcgcc cggctccggc tggcccgata cgattccgac
      661 cggcttgttg tagatgacgg tgagcttcgc cgccgtcgcc tgcgcgtcgc tgaaagatac
      721 cgtctgcccg cgcgtcagct tgcggcccgc gtccgtgacc gtctcgccgt cgacgcgcac
      781 gcggccttcc ccgatcagcg cgtcggcctc gcggcgcgaa cacacgccgc tctgcgccag
      841 ccatttgttg agccggatcg gctcggcgcc gtcataggtg aaggtgtcca tgcgggctct
      901 gtactgaaaa agcggtgtcc gcgccatcgg aaacgcgtta ccacatgcga actgactttt
      961 tatgcgcggt gtggctagct gcgcgggcgg cgagggttaa gacggcgagg gcgagaccat
     1021 gacgaagacg gagatgatgc gcgcgatgaa gggcctggcg tgcctggcgg cggcgatcgc
     1081 gctgccggcc tgcgcgaagg cgcaggagat cgcggcgggc aaggcggctt tcgacgccga
     1141 ctgttcgctg tgtcacgacg ccagcccggc ccgcgccacg ttccaggggc cgccgctgtt
     1201 cggcgtcgtc gggcgcaaga cgggcgccgt atcgggcttc gcctattcgg cggcgctgaa
     1261 aagcgcgggc gccaaagggc aggtctggac ggcggcggcg ctggaccgct tcctgaccga
     1321 tccgcagaag gccatgcccg gcacggccat gccggtcagc gtcgccgacg ccaaaacgcg
     1381 caaggccatc atcgcctatc taagcagcct gaacggcggc aaggcgaagg cggcgcccaa
     1441 accggtcatc aagccggtca tgaagccggc ggcatcggcc tccgccgctc aggactggcg
     1501 cgcggacgcg ccgggcgtcg cgcataccgt gaccgccgcc gacctgcccg cgccctacgc
     1561 cacgcagtcg gcgggcaatt cggcgcacta cgccgcgccc gatgcgggca agctgcccgt
     1621 ggttccggcg ggcttcgccg tgtcggtgtt cgccgacggg ctggacgggc cgcgcctgct
     1681 gcgcgcggcg ccgaacggcg acctctacgc ggtggcgtcg gagacgggca aggtgctggt
     1741 ctggcgcaat gcgggcgggc gtctggccgc gcagccggaa accttcgcca cgggcctgcg
     1801 tcagccgttc ggcgtcgcct tctatccgct ggataagccg cggttcgtct atatagccaa
     1861 tgtcgattcc atcgtgcgcg tccccatcgg cggcggcgcg gcgcaaaccg tggtggccca
     1921 tctcagcacg gcgggcggcc atcatacgcg cgacatcgcc ttctcgccgg atgggcgtta
     1981 catgtacgtc tcggtcggat cgggcagcaa tgtcgcggaa aacatgggcg ccatgcccgc
     2041 gggctggatc gcgtcgcacg cgccgggcga accgtggggg tccgaggccg ggcgcgcgat
     2101 ggtgctgcgc ttcacgccgg acggtcagga ccggcatgtg gtcgccaccg gcatccgcaa
     2161 ctgcgtgagt ctggccgtgc ggcccggttc ggacgcgctc tattgcgtga ccaacgaacg
     2221 cgacgcccta ggcgacaatc tcgtgcccga ctactttacg caggtgaagg acgggcagtt
     2281 cttcggctgg ccgtggtggt atctgggcga ccatcaggat ccgcgccaca agggcgagcg
     2341 cggcgacctg aaaggccatg tcagcctgcc cgacacgctg ttcgcctcgc actccgcgcc
     2401 gctggggctg gtgttttacc ggcccgcgcc gggcggcgcc gccaacttcc cggcggacta
     2461 cacgggcgac gcgtttatca ccctgcacgg ctcatggaac cgggcgcagc gcaccggctc
     2521 gaaactcgtg cgggtgcgct tcgatcacgg caggccgtcc gccagctatg aggacttcat
     2581 gaccggcctg atcgtcgacg accacaccgt caacggacgg cccgtcggcg tgacggtcgg
     2641 cccggacggc gcgctctacg tctccgacga cgcgggcggc cggatctggc gcatcgcgcc
     2701 ggtggggaag taagggtagg gaaccacgga aaacacggaa agcacggacg ggagcctgat
     2761 cctgcgcgcc ctgaggctga tcgacagcgc gcttcgcgcg cgaaaatccc tcggcccgtc
     2821 cgacaagcgt tggtgccttt cgtgattttc gtggttccga tcctgcgacc agtggcccaa
     2881 cgaaaatggg ccggcgtcgc cgccggccca tctgtggtcg tcgttcccgg ggcagggccc
     2941 cgggtccgaa gctgtt
//
LOCUS       DASVLL010000629         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_528924,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000629 DASVLL010000000
VERSION     DASVLL010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528924"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF402_10330"
     CDS             complement(<1..408)
                     /locus_tag="VF402_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX7800738.1"
                     /translation="MTFLGKIPFWLKTVIAFIAGVAVALIFGKAALPVFDPIGDTFIR
                     AIKMLVAPLVFFTIAASVARLGHQEGGHEEGGRREGGGGRAVRLGGYTIVWFMITSAV
                     AVSVGLALGTIFQPGLGVGHLPLDPSAVKPVPGP"
     gene            536..1621
                     /gene="aroC"
                     /locus_tag="VF402_10335"
     CDS             536..1621
                     /gene="aroC"
                     /locus_tag="VF402_10335"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX7800739.1"
                     /translation="MSHNTFGHLFRVTTWGESHGPALGCVIDGCPPGVPLSEADIQGA
                     MDRRKPGGSRFVTQRQEADAAKILSGVFEGVTTGTPISILIENTDQRSKDYGEIARQF
                     RPGHADYTYFAKYGVRDYRGGGRSSARETAARVAAGAVALKVLKTLVGDIQVRGALVQ
                     LGAHRIERSRWDWAQVGENAFFCPDPGSVAGWEAYLDGVRKAGSSIGAIVEVQAEGVP
                     AGWGAPIYGKLDSELAAALMSINAVKGVEIGEGFGAAELSGEENADEMRIIDGQPVFL
                     SNHAGGILGGISTGQTVTARLALKPTSSILTPRRSIDVEGHEVELRTKGRHDPCVGIR
                     AVPVAEAMMSCVLLDAFLRHRGQTGGA"
     gene            complement(1762..>1878)
                     /locus_tag="VF402_10340"
     CDS             complement(1762..>1878)
                     /locus_tag="VF402_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX7800740.1"
                     /translation="QPKDDARQAANTQAVIEYCLAHPRWRMSVQTHKLVGLK"
BASE COUNT          337 a          644 c          614 g          283 t
ORIGIN      
        1 cggccccggc acgggtttga ccgccgacgg atcgagcggc aaatggccca cgcccagccc
       61 cggctggaaa atcgtcccca gcgccaaccc gaccgacacg gcgacggccg aggtgatcat
      121 gaaccagacg atggtgtagc cgcccagccg caccgcgcgt ccgcccccac cttccctacg
      181 tccgccttcc tcatgtccgc cttcctgatg gcccagccgc gccaccgacg ccgcgatggt
      241 gaagaagacc agcggcgcga ccagcatctt gatcgcccgg atgaaggtgt cgccgatcgg
      301 gtcgaacacc ggcagggccg ccttgccgaa gatcagcgcc acggcgacgc ccgcgataaa
      361 ggcgatgacc gtcttgagcc agaacggaat tttaccgagg aatgtcatga tgccgtttcc
      421 tgagcaacca atcccgccac atatgggcct gcgccgcgcc gaatgcacac taaaaaattt
      481 gccgcgaggc gtaaacgcgg ctaaagccaa ggcacgaaat ttcaggcttt gagacatgtc
      541 gcacaacacc ttcggccatc tgttccgcgt caccacctgg ggcgaaagcc acggcccggc
      601 gctcggctgc gtgatcgacg gctgcccgcc gggcgttccc ctgtccgagg ccgacatcca
      661 gggggcgatg gaccggcgca agcccggcgg ctcgcgcttc gtcacccagc gtcaggaagc
      721 cgacgcggcc aagatcctgt ccggcgtgtt cgagggcgtg accaccggca cgccgatctc
      781 gatcctgatc gagaacaccg accagcgctc caaggactat ggcgagatcg cgcgccagtt
      841 ccggcccggc cacgccgact acacctattt cgccaagtac ggcgtgcgcg actatcgcgg
      901 cggcgggcga tcctcggcgc gcgagacggc ggcgcgcgtg gcggcgggcg cggtggcgct
      961 gaaagtcctg aaaacgctgg tcggcgacat tcaggtgcgc ggcgcgctgg tgcagcttgg
     1021 cgcgcacagg atcgagcgct cgcgctggga ctgggcgcag gtcggtgaaa acgccttctt
     1081 ctgccccgat ccgggcagcg tcgcgggctg ggaagcctat ctcgacggcg tgcgcaaggc
     1141 cggatcgtcc atcggcgcca tcgtcgaggt gcaggccgag ggcgtccccg ccggctgggg
     1201 cgcgccgatc tacggcaagc tcgattccga actggccgcc gccctgatga gcatcaacgc
     1261 ggtcaagggc gtcgagatcg gcgaaggatt cggcgcggcg gaactgtccg gcgaggagaa
     1321 cgccgacgaa atgcgcatca tcgacggcca gccggtgttc ctgagcaacc acgcgggcgg
     1381 catattgggc ggcatctcta cgggtcagac cgtgacggcg cggctggcgc tgaaaccgac
     1441 ctcctcgatc ctgacgccgc gccgcagcat cgacgtcgag ggccatgagg tcgaactgcg
     1501 caccaagggc cgccacgacc cctgcgtcgg catccgcgcc gtgccggtgg ccgaggcgat
     1561 gatgagctgc gtgctgctcg acgccttcct gcgccatcgt ggccagacgg gcggcgcata
     1621 agccggtcgg gctgaaatag ttatggggcg tgggggcgat cgcccccaca tcttcactat
     1681 ccacgagaaa gcccggcctg aaggccgggc tttctcgtgg ataaggaaaa ggcttggggc
     1741 caatggcccc aaaccccata actatttcaa tcccaccagc ttgtgcgtct gcacgctcat
     1801 gcgccatcgc gggtgcgcaa ggcagtactc gatcaccgcc tgcgtattcg ccgcctgtcg
     1861 cgcgtcgtcc ttcggttg
//
LOCUS       DASVLL010000630         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_259411,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000630 DASVLL010000000
VERSION     DASVLL010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259411"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VF402_10345"
     CDS             complement(<1..412)
                     /locus_tag="VF402_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7800741.1"
                     /translation="MRPPETRSYVSAKREAAAAKKRQAVIEAAGRLMAEGPAMMSMEA
                     VARAAGVTRLTVYKQFGSRRGLLEAVFDDNAARSGMVRQMLAAMNHPAPCRGLDDVID
                     VMCGFWGAHPQFARLHDAGASDPEFAEALNERNQR"
     gene            549..944
                     /locus_tag="VF402_10350"
     CDS             549..944
                     /locus_tag="VF402_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800742.1"
                     /translation="MQIWIILAISVHALAAVFWMATTGALARSGGLGAEALFPRQMIA
                     IAIVVLTGGYLWSALHTGGFGAYEEVLAGGAALAVIAALIQAIGVGVSMGRLKTAREA
                     ALKRITLCHRLAGPLLALCLVCMVAARFV"
     gene            complement(1047..1928)
                     /gene="aguB"
                     /locus_tag="VF402_10355"
     CDS             complement(1047..1928)
                     /gene="aguB"
                     /locus_tag="VF402_10355"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEX7800743.1"
                     /translation="MTRTVTVAALQTAYGPDMAANIARTEALVREAAALGAQIILPSE
                     LFQGEYFCVSQEERWFATAFAWREHPCVLAMQKLAAELDVVIPTSIYEKEGPHYFNSL
                     VMIDAGGDLMGVYRKSHIPDGPGYQEKYYFRPGDTGFEVWDTKYAKVGVGICWDQWYP
                     EAARAMTLMGAEVLMYPTAIGSEPHDADLDTAAPWQRVMQGHAVANVIPVVASNRIGQ
                     ESLIAPQSGQGQSFYGHSFIANHRGDKLAEFGSVEEGVLTARFDLDYLNTHRAAWGFF
                     RDRRPELYTALASARPA"
     gene            complement(1925..2941)
                     /locus_tag="VF402_10360"
     CDS             complement(1925..2941)
                     /locus_tag="VF402_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX7800744.1"
                     /translation="MARYVPAEWEAHKAMWLGFPSHENLWRDDLIPAQAEAAALARTL
                     SELGGERVRLMTMGEAARAAAQELLGDVERLDIVDGRFGDIWFRDTGPIYVAEDGKAL
                     PVAFRNNGWGGKYRLPHDDEVAAQIAAADGFAPDARGFILEGGALDHDGLGTVLTTRQ
                     CLLNPNRNPGWSIDAAEKALGEALGVRKAIWLDDGLLHDHTDGHVDNLARFVAPGVVA
                     CPVAFGRDDPNAAVYDHTARLLAEATDARGEKLQVVRIPSPGRIVDEDGEIIPASHMN
                     FLIGNDCVVVPVYAERPGELAVEALQSLFPERNVLGLSSRAILTGGGAFHCISQQVPV
                     VNPA"
BASE COUNT          520 a         1031 c         1020 g          592 t
ORIGIN      
        1 gccgttgatt tctctcgttc agcgcctcgg cgaattccgg gtcgctggcc ccggcgtcat
       61 gaaggcgggc gaattgcgga tgcgcgcccc agaagccgca catgacgtcg atcacatcgt
      121 ccagaccccg acagggtgcg ggatggttca tcgccgccag catctgccga accatgccgc
      181 tgcgcgcggc gttgtcgtcg aacacggcct ccagcaggcc gcggcgcgaa ccgaactgct
      241 tatagacggt caggcgcgtg acgcccgccg ccctggcgac cgcctccatc gacatcatgg
      301 ccgggccttc ggccatcagc cgtccggcgg cttcgatcac cgcctgacgt tttttggcgg
      361 cggcggcttc acgcttggcg ctgacatagg aacgcgtttc tgggggccgc atttctgggg
      421 gacgcgtttc tggctgcgaa gggggctccg gcgacatttt agaccttgaa attcatttta
      481 ctgcgtgtat atccattatt tcagtaagca gcgtgtacat ttattttccg cttccaacgg
      541 agatttgcat gcaaatctgg atcattctag ccatttccgt ccatgccctc gccgctgttt
      601 tctggatggc gacgaccggc gctttggcgc gcagcggcgg tctgggcgcg gaggcgctgt
      661 ttccaagaca gatgatcgcc atcgcgatcg tcgtgctgac aggcgggtat ttgtggagcg
      721 cgctccacac gggtgggttc ggcgcctatg aagaggttct ggccggcgga gcggcgctgg
      781 ccgttatcgc cgccctgatc caggccatag gcgtcggcgt cagcatgggc aggctcaaaa
      841 cggcgcgcga ggcggccctg aagcgcataa cgctctgtca ccgcctggcc ggtcccctgc
      901 tggcgctatg cctcgtctgc atggtcgcgg cgcgcttcgt ttagagcgtt ttccgatctg
      961 attgcatcag atcggaaaac gctctaactt tatgctttta cgcgcttttt aatccgaaaa
     1021 gtgcgtcaca cttttcggaa agcgctctag gccgggcggg ctgaggccag cgcggtgtaa
     1081 agctccggcc tgcggtcgcg gaagaagccc caggcggcgc ggtgcgtgtt gaggtaatcg
     1141 aggtcgaagc gcgcggtcag cacgccctcc tcgacgctgc cgaactcggc cagcttgtcg
     1201 ccgcgatgat tggcgatgaa gctgtggcca tagaagctct gcccctgccc gctctgcggc
     1261 gcgatcaggc tttcctgacc gatgcggttc gaggccacca ccgggatcac attggcgacg
     1321 gcgtggccct gcatgacgcg ctgccacggc gcggcggtgt cgaggtccgc gtcgtgcggt
     1381 tcggagccga tggcggtcgg gtacatcagc acctcggcgc ccatcagcgt catggcgcgc
     1441 gccgcctccg ggtaccattg gtcccagcaa atgccgacgc cgaccttggc gtacttggtg
     1501 tcccagacct cgaagccggt gtcgccggga cggaaataat acttttcctg atagccggga
     1561 ccgtccggga tatggctctt gcgatagacg cccatcagat cgccgcccgc gtcgatcatg
     1621 accagcgaat tgaaatagtg cggcccttcc ttctcgtaga tcgaggtcgg aatcaccacg
     1681 tccagctccg ccgccagttt ctgcatggcc agcacgcacg gatgctcgcg ccacgcaaag
     1741 gcggtcgcga accagcgctc ctcctggctc acgcagaaat attcgccctg aaacagctcg
     1801 gacggcagga tgatctgagc gcccaaggcc gccgcctcgc gcaccaacgc ctcggttctg
     1861 gcgatattgg ccgccatgtc cgggccgtag gcggtttgca gggcggcgac ggtgacggtg
     1921 cgggtcatgc gggattcacc acgggaacct gttggctgat gcaatggaac gcgccgccgc
     1981 cggtcaggat ggcgcgcgag gacaggccca gcacgttcct ctccgggaac aggctttgta
     2041 gcgcctcgac cgccagctcg cccggacgtt cggcatagac cggcacgacc acgcagtcgt
     2101 tgccgatcag gaagttcata tgggaggccg ggatgatttc gccatcctcg tccacgatgc
     2161 ggcccggcga cgggatgcgc acgacctgca atttttcgcc gcgcgcgtcg gtcgcttcgg
     2221 ccagcagccg cgccgtgtgg tcatagacgg cggcgttggg atcgtcgcgc ccgaacgcca
     2281 ccggacaggc cacgacgccc ggcgccacga agcgcgccag attgtcgaca tggccgtcgg
     2341 tgtgatcgtg cagcaggccg tcgtccagcc agatcgcctt gcgcacgccc agggcttcgc
     2401 ccagcgcctt ttcggcggcg tcgatcgacc agccgggatt gcggttcgga ttgagcaggc
     2461 actgacgcgt ggtcaaaacc gtgccgaggc cgtcatggtc gagcgcgccg ccttcgagaa
     2521 tgaagccccg cgcgtcgggc gcaaagccgt cggcggcggc gatctgcgcc gcgacctcgt
     2581 cgtcgtgggg cagtcgatac ttgccgcccc agccgttgtt gcggaaggcg accggcagag
     2641 ccttgccgtc ctcggcgaca tagatcgggc cggtgtcgcg gaaccagatg tcgccgaaac
     2701 gcccgtcgac gatgtcgagc ctctccacgt cgcccagcaa ctcctgcgcc gccgcgcgcg
     2761 cggcctcgcc catcgtcatc agccggacgc gctcgccgcc cagctcgctt aaggtgcgcg
     2821 ccagcgccgc cgcctcggcc tgcgccggga tcaggtcgtc ccgccacagg ttttcatggc
     2881 tgggaaagcc cagccacatc gccttatgcg cttcccattc cgcgggaacg taacgtgcca
     2941 tgatcgatct cataaagtgg ggacgctgcg gccctcttag catcaagccc ggccaacggt
     3001 caccccctaa tgtggatacg aaaacggctc ccggatggcc gggagccgtc atgttcagcg
     3061 tccgatgatc aggatcagaa ctgggtgtcg agggtcagca tggtcgaacg cgactgaccc
     3121 ggatagtagt agttgccgct gtcgtagaag gcgttgcccg aat
//
LOCUS       DASVLL010000631         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_42170, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000631 DASVLL010000000
VERSION     DASVLL010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42170"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VF402_10365"
     CDS             complement(<1..628)
                     /locus_tag="VF402_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX7800745.1"
                     /translation="MLALPLSWPLKNKRVALIGDGAWLTRKLTLLRRTPAELRVYAPF
                     GHAASSAGETVEQRWPDAADLADAALIVVAFADRDLAERGAALARQTRAPLNVVDYPD
                     LSDFHFPAIIDRGTLSIGVASGGTAPVLAREARRRIEAAVPPSETHVAAFAEALSAPL
                     RERFGDVDDRRRVWEQVLASPAAETARSGRIGEAVEQALRAVDAREPRK"
     gene            complement(727..1074)
                     /locus_tag="VF402_10370"
     CDS             complement(727..1074)
                     /locus_tag="VF402_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7800746.1"
                     /translation="MRHAGSCHCGAVAFEAEGDFDSALSCNCSICRRKGSLLAFIPAS
                     DFTLTTPKDKVATYTFNTHVIRHHFCPACGVSPYAEAPAPDGTPTVALNLRCLPDIDL
                     DALSLTHWDGASH"
     gene            1176..1613
                     /locus_tag="VF402_10375"
     CDS             1176..1613
                     /locus_tag="VF402_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEX7800747.1"
                     /translation="MSSEHALEARRKIREDAAAIGVDEAFISRLVDEFYRRVRLHPHL
                     GPVFDGAVEDWPEHLAKLKLFWQSVALNAGVYTGRPMQAHMRVPSIEPAHFGEWLHLF
                     EQTLRDLAEDERAVEYFMMRANRIGESLKLGLFYRPEQDLRGA"
BASE COUNT          278 a          505 c          601 g          269 t
ORIGIN      
        1 ccttgcgagg ctcgcgcgcg tcgacggcgc gcagcgcctg ctccacggct tcgccgatgc
       61 ggccggaacg ggcggtctcg gcggcggggg aggccagaac ctgctcccag acgcggcggc
      121 ggtcgtcgac atcgccgaac cgttcgcgca agggcgcgct cagcgcttcg gcgaacgcgg
      181 cgacatgggt ttcggacggc ggcacggcgg cctcgatgcg gcggcgcgcc tcgcgcgcca
      241 gcaccggcgc ggttccgccc gaggcgacgc cgatgctcag ggtgccgcga tcgatgatgg
      301 ccgggaaatg gaagtcgctg aggtcgggat agtcgaccac gttcagcggc gcgcgagtct
      361 ggcgggcgag ggcggcgccg cgctcagcca ggtcgcgatc cgcgaacgcc accacgatca
      421 gggcggcgtc cgcgaggtcg gcggcgtcgg gccagcgctg ttcgacggtt tcgccggccg
      481 aggacgcggc atgtccgaac ggcgcataga cacgcaattc cgccggggtg cggcgcagca
      541 gggtcagttt gcgcgtcagc cacgcgccgt cgccgatcag cgccacgcgc ttattcttca
      601 acggccagga caggggcagg gccagcatgg gcggcgaact ccggggaacc gagttcgcct
      661 ttttagtctc tcgcgcctgc gaagaaaagc gatggtttgt cgtgagagcg tttagatcaa
      721 ctgactctaa tggctggcgc cgtcccaatg agtcagggac agcgcgtcga gatcgatatc
      781 gggcaggcag cgcaggttga gcgccacggt cggcgttccg tcgggcgccg gggcctcggc
      841 atagggcgat acgccgcagg ccgggcagaa atggtggcgg atgacgtgcg tgttgaaggt
      901 gtaggtcgcg accttgtcct tcggcgtggt cagggtgaag tcgctggccg ggataaaggc
      961 cagcagcgaa cctttgcggc ggcagatcga gcagttgcag ctcagggcgg agtcgaaatc
     1021 gccctcggcc tcgaaggcga cggcgccgca gtggcagctt ccggcatgac gcatggtttt
     1081 tctccgtttc aggcgcccca tcaggcgccc catcaggcgc cctatgacaa cgcgtcacat
     1141 ggtcaaaagg cgtcagcagg gttagactgc cgtccatgag ttccgaacac gcgcttgaag
     1201 cccgccgcaa aatccgtgaa gacgccgccg ccatcggcgt cgacgaagcc tttatctccc
     1261 ggctggtcga tgagttttac cggcgggtgc ggctgcatcc gcatctgggg ccggtgttcg
     1321 acggcgcggt cgaggactgg ccggagcatc tggccaagct caagctgttc tggcagtcgg
     1381 tggcgctgaa cgccggggtc tataccggac ggccgatgca ggcgcatatg cgggttccga
     1441 gcatcgaacc ggcgcatttc ggcgaatggc tgcacctgtt cgagcagacc ctgcgcgatc
     1501 tggccgagga cgagcgcgcg gtcgaatatt tcatgatgcg cgccaaccgg atcggggaga
     1561 gcctgaaact gggcctgttc taccgcccgg agcaggattt gcgcggggcg tgaaaataga
     1621 ctaagctagt cggactaagc ttatgaggtc cgt
//
LOCUS       DASVLL010000632         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_474677,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000632 DASVLL010000000
VERSION     DASVLL010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474677"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VF402_10380"
     CDS             complement(<1..455)
                     /locus_tag="VF402_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX7800748.1"
                     /translation="MFFSVLFSVLASAAPVAAPPPPGVWTPDRGDGTYLNPVLQGDYS
                     DPDAIRVGGDFYLTSSSFSNVPGLPVLHSKDLVNWEIVGHALPRLAPDDHYRTPRRGG
                     GVWAPAIRYHAGRFYIYYPDPDFGIYMVSAADPAGPWSAPVLVDDDKGA"
     gene            complement(471..2249)
                     /locus_tag="VF402_10385"
     CDS             complement(471..2249)
                     /locus_tag="VF402_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800749.1"
                     /translation="MKILLFCAAALAMLPPCMAQAQTGAAPTTVVVQGQKGARRPAVW
                     FRAESQHFVIYSDTRREDVTTLLNALEKFRYVLRSYLREDAAGDDAGPKTEITYLAHE
                     QDLDVIRPGGPDYAIGLYSACQDADIGVGVHMYADQVKALPLEKQPDNEGLGYIFEAY
                     ARHYSYQHSDLRQPTWFIDGFAHYFSTARFDGNEAIVGMAPAAFARYLDLIGAATTYS
                     LDYRDVLQQKEDNGRNAAGPSGVRNEYQARAWLLTHYILSSPDNRRRFAAYLAAVAAG
                     QPPVAAWKTAFAMTPGQLDQALWRYRLRDLQAMKLVFKSLPAAAISFQALPASARSIQ
                     LLRTALRACPDAAYGHSLLTRLRAEAPKFPDSDLAQLTLARAEIEWGDPNAALPYLTR
                     ATAGASTDAEAFVLLGRADIALAAGLEERARQARLEQAMQALGRASVLDPAAPETGWW
                     FYRAVALSGRMSEDGAAAILSAWRGAPDVDLYALHAGLALAWLGRTDEAMAALRTVAD
                     NPRDPALAPMAQGWIGRLNAGVDKAALVAGMGAAPPADAGQTQWTLAGADIFAAVKAA
                     ADAQDANDALSTADPQSDQDSQLPDN"
     gene            complement(2246..>3595)
                     /locus_tag="VF402_10390"
     CDS             complement(2246..>3595)
                     /locus_tag="VF402_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800750.1"
                     /translation="AVCDDHVRMVADILQHGGTSYDLEDHKAIVAGAGAFLDQWGFRT
                     AFPTTESYFLSVGEKQYESWRNYLENIRICDSVDMAAISGWESTAIENHSGIVDNLRY
                     FHAAPERISVSLQPVRPVAKQRRMTYAEGEAALLDLWLLNDTGRAAAGGLTLSVTDPA
                     GRTVRVGRYDAPAQTADVFSYLVAEKVATPPLTGEGAHTVRFELDGHPGATFLRRLWV
                     VRTDVAPARPLRVALSGVAGSFRERLTALPGVTFEDFAAGGAYDAVIASGLKADEIER
                     RQIGDQTGNEAAPKKGAAPKLVPGELPEDVLAAARGGLPLLVMAPEDGLADGIATQLA
                     RDGLFGYAGQVGDLRAPWMGNWNVLRAHPVFAGIPADGCAALWHQVDGHAANGLKVSG
                     EGVEIIAAYSRDHDRFMGASCFTVRKGAARLLFHRMPDMVAPLQTRFLINALNWLTT"
BASE COUNT          553 a         1275 c         1218 g          549 t
ORIGIN      
        1 atggcgccct tgtcgtcatc gaccagaacc ggcgcgctcc acggccccgc cggatcggcg
       61 gcgctgacca tataaatccc gaagtcggga tcgggataat agatgtagaa gcgcccggcg
      121 tgataacgga tcgcgggcgc ccacacccca ccgccgcgcc gcggcgtccg gtaatgatcg
      181 tcgggcgcca gcctcggcag ggcgtggccg acgatctccc agttgaccag atccttcgaa
      241 tgcagcaccg gcaggccggg gacattggag aaggaggacg aggtcaggta gaaatccccg
      301 cccacccgga tcgcgtccgg gtcggaatag tcgccttgca gcaccggatt cagataggtt
      361 ccgtctcccc ggtccggcgt ccagacgccc ggcggcggcg gcgcggctac cggagcggcc
      421 gacgccagaa cgctgaaaag aacgctgaaa aacatgggtt ttcctcttcg tcagttgtcg
      481 ggcagctggc tgtcctgatc gctttgcggg tcggccgtgc tgagcgcgtc gttggcgtcc
      541 tgcgcgtcgg cggcggcctt gacggcggcg aagatgtcgg cgccggccag ggtccactgc
      601 gtctgcccgg cgtcggcggg cggcgccgcg cccatgcccg ccacgagggc cgccttgtcc
      661 acgcccgcat tgaggcgtcc gatccagcct tgcgccatcg gggccagcgc cggatcgcgc
      721 ggattatcgg ccacggtgcg cagcgccgcc atcgcctcgt ccgtccgccc cagccaggcc
      781 agcgccagac cggcgtgcag cgcgtagaga tcgacgtccg gcgcgccgcg ccaggcggac
      841 aggatcgccg ccgccccgtc ctcgctcatc cggccgctca gcgcgacggc gcggtagaac
      901 caccagccgg tttccggcgc cgccggatcg agcaccgacg cgcgccccag cgcctgcatg
      961 gcctgttcga gccgcgcctg acgcgccctc tcctccagcc ccgccgccag cgcgatgtcg
     1021 gcccggccca gcaggacgaa agcctccgcg tccgtcgagg cgcccgccgt ggcccgcgtg
     1081 agatacggca gggccgcgtt cggatcgccc cattcgatct cggcgcgcgc cagggtcagt
     1141 tgcgccagat cgctgtcggg aaatttcggc gcctcggcgc gcagccgcgt cagaaggctg
     1201 tggccatagg ccgcgtccgg gcaggcgcgc agcgccgtgc gcaacaactg gatcgaccgg
     1261 gccgaggcgg gcagcgcctg aaagctgatc gcggcggcgg gcaggctttt aaagaccagc
     1321 ttcatggcct gcaaatcgcg caggcgatag cgccacagcg cctgatcgag ctggcccggc
     1381 gtcatggcga aggcggtttt ccacgccgcg accggcggct gtccggcggc gacggcggcc
     1441 agataggccg cgaacctgcg ccggttgtcg ggcgaggaca ggatatagtg ggtcagcagc
     1501 caggcgcgcg cctgatattc gttgcgcacc cccgaaggcc cggcggcgtt gcggccgttg
     1561 tcctccttct gttgcagcac gtcgcggtaa tcgaggctgt aggtggtcgc ggcgccgatc
     1621 aggtccagat agcgcgcgaa cgccgccggg gccatgccga cgatggcctc gttcccgtcg
     1681 aagcgcgcgg tcgaaaaata atgcgcgaag ccgtcgatga accaggtcgg ctggcgcagg
     1741 tcgctgtgtt gatagctgta atgacgcgca taggcttcga agatatagcc gagcccctca
     1801 ttgtccggct gcttctccag cggcagggcc ttgacctgat cggcgtacat atgcacgccc
     1861 acgccgatgt ccgcgtcctg acacgccgaa tagaggccga tcgcatagtc cggcccgccc
     1921 ggccggatga cgtcgagatc ctgttcgtgg gccagatagg tgatctcggt cttgggtccg
     1981 gcgtcgtcgc ccgccgcgtc ctcgcgcaga tagctgcgca gcacgtagcg gaacttttcc
     2041 agcgcgttca gaagcgtggt cacgtcctcg cggcgcgtat cgctgtagat gacgaagtgc
     2101 tggctttcgg cgcggaacca gacggcgggg cggcgcgcgc ccttctggcc ctgcaccacc
     2161 acggtcgtcg gggcggcgcc cgtctgggcc tgagccatac agggcggcag catcgccagg
     2221 gcggcggcgc aaaacagcag gatcttcatg tcgtcaacca gttcagcgcg ttgatcagaa
     2281 agcgggtttg cagcggcgcg accatgtcgg gcatgcggtg gaacaaaagc cgcgccgcgc
     2341 cctttcgcac ggtgaagcac gacgcgccca tgaagcggtc gtggtcgcgc gaataggcgg
     2401 cgatgatctc gaccccctcg cccgacacct tcagcccgtt ggcggcgtgg ccgtcgacct
     2461 gatgccacag ggccgcgcaa ccgtcggcgg gaatccccgc gaacaccgga tgcgcgcgca
     2521 gcacgttcca attgcccatc cacggcgcgc gcaggtcgcc gacctgcccg gcgtagccga
     2581 acagcccgtc ccgcgccaac tgcgtcgcga tcccgtcggc cagcccgtcc tccggcgcca
     2641 tgaccagcag cggcagcccg ccccgcgcgg cggccagcac gtcttcgggc agttcgcccg
     2701 gaaccagctt cggcgccgcg ccctttttcg gcgccgcctc gttgccggtc tggtcgccga
     2761 tctggcggcg ttcgatctcg tccgccttga ggccggaggc gatcaccgcg tcataggcgc
     2821 cgcccgccgc gaaatcctcg aaggtcacgc cgggcagcgc cgtcagccgc tcgcggaagg
     2881 atccggccac gcccgacagc gccacgcgca gcggacgggc gggcgccacg tccgttctga
     2941 ccacccagag cctgcgcagg aaggtcgcgc cgggatgtcc gtccagctcg aagcgcaccg
     3001 tatgcgcacc ctcgccggtc aggggcggcg tcgcgacctt ttcggccacg aggtagctga
     3061 agacgtccgc cgtctgcgcg ggcgcgtcgt agcgtccgac ccgcaccgtc cggcccgccg
     3121 gatcggtcac gctcagcgtc agcccgcccg ccgccgcccg gcccgtatcg ttgagcagcc
     3181 acaggtccag cagcgccgcc tcgccctcgg cataggtcat gcgcctttgt ttggcgaccg
     3241 gacgcaccgg ttgcaggctg accgaaatgc gctccggcgc ggcgtggaaa tagcgcagat
     3301 tgtcgacaat gccggaatgg ttctcgatgg cggtcgattc ccagccgctg atcgccgcca
     3361 tgtcgacgga atcgcagatg cggatattct ccagatagtt ccgccacgac tcgtactgct
     3421 tttccccgac gctgaggaaa tagctttcgg tggtcgggaa cgccgtgcgg aagccccact
     3481 gatcgaggaa ggcgcccgcg cccgcgacga tcgccttgtg gtcctcgagg tcgtaactgg
     3541 tcccgccgtg ttgcaggatg tcggcgacca tcctcacatg gtcgtcgcag accgc
//
LOCUS       DASVLL010000633         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_259596,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000633 DASVLL010000000
VERSION     DASVLL010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259596"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF402_10395"
     CDS             <1..240
                     /locus_tag="VF402_10395"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479447.1"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7800751.1"
                     /translation="GNKPSRRRIAYERLILDALNGNNAQFVRRDEVEAAWIYVDGIVE
                     RWKEAGMKPQVYQAGTVGPVSAYTLMDRDGRMWND"
     gene            233..985
                     /gene="pgl"
                     /locus_tag="VF402_10400"
     CDS             233..985
                     /gene="pgl"
                     /locus_tag="VF402_10400"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX7800752.1"
                     /translation="MTDDACEITPLAPNARLLAFDDKAEAGAYVADQIAEALQQALDE
                     RGRAVWIGCGGTTPKPVYERLNEAVLDWSQVTLAQVDERWVSTDSPDSNTRMMREALT
                     PALDAGLTLESLMQDGHDQQACADKAEAVLYDLGGGKAPVFDFALLGMGPDGHYASIF
                     PQHPVNAVVYDSPRLVLPVAATDGTIEPKLPRITLTVPCIDSSRRIVFFITGQAKRDV
                     LNSMIRDTDPYLSPIGAFLAQCPVPVDFVWTA"
     gene            987..2801
                     /gene="edd"
                     /locus_tag="VF402_10405"
     CDS             987..2801
                     /gene="edd"
                     /locus_tag="VF402_10405"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEX7800753.1"
                     /translation="MTELHPVLHKVTQRIIERSKATRAQFMARADRYKASEPRRKKLS
                     CAYYAHVVAAASDNDKLQAALDAVPNLGVVTAYNDMLSAHQPYHDYPEKIRDYARKAG
                     ATSQVAGGVPAMCDGVTQGRPGMELSLFSRDVIAMGAAIALSHDAFDAALMLGICDKI
                     VPGLVIGALSFAHLPVIFVPSGPMSSGLPNPEKAKIRTLYALGEVGRDALLAAEMASY
                     HAAGTCTFYGTANSNQMLMEMMGLHVPGAAFVHPSTPLRDALTAAAVERAARTAENSK
                     DPHRLADVLTEKAFVNGVVGLMATGGSTNHALHLPAMARAAGVILTWEDMNDLSAVVP
                     LLARVYPNGSADVNHFHAAGGVGFVIRELLSAGLLHEDVETVWGHGLGLYAREPVLQD
                     GALIWRDLPQNSLDEAVLRPISNPFQPEGGLRECTGNLGHAIVKVSAVKPEHQIVEAP
                     CVVFDDQDDFLAAFKAKDLPEGDFICVVRFQGPKANGMPELHSLTPSLSSFLDAGRKV
                     ALVTDGRMSGASGKVPAMIHLTPEALDGGPIARLKTGDVLRVDCEKGTLDYLGDATGF
                     ANRTDAERPAEADGVGRELFAHMRRQVGPADQGGAVFW"
     gene            2806..3813
                     /locus_tag="VF402_10410"
     CDS             2806..3813
                     /locus_tag="VF402_10410"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7800754.1"
                     /translation="MTAHLKSGSVTGAAIRGLVGDIGGTNARFAIAERKDGKTRLAHF
                     KSLETADHADFYAALTAYFELVGEKPGFDYACVAVAGPVKDGAIKFTNLDWEVREDRL
                     AQVAGAKTAKLINDYAALAYCLPHLSDSDTKTIGPVRTGFGDVHAVMGAGTGFGASVL
                     VGGPYGPYCLSTESGHISFGPVNDYETEMLRFLRRKHGRVTVEMMLSGPGLVNLYQAI
                     ASIRGEVAEELTPAQITHLEGDDAQGCRYTVEAFLDIMASVCGDLALAHGATSGMFIA
                     GGIAPRLIKHIDELRFRARMEAKAPMAGLVASIPSSIIIHPYAALLGSANALTDHQIL
                     A"
BASE COUNT          683 a         1394 c         1361 g          649 t
ORIGIN      
        1 ggcaacaagc cgtcgcgccg ccgcatcgcc tatgagcgcc tgatcctcga cgcgctgaac
       61 ggcaacaacg cgcaattcgt gcgccgcgac gaggtcgagg ccgcgtggat ctatgtcgac
      121 ggcatcgtcg agcgctggaa agaggcgggc atgaagccgc aggtctatca ggcgggcacg
      181 gtcggcccgg tgtcggccta cacgctgatg gaccgcgacg ggcgcatgtg gaatgactga
      241 cgacgcctgc gagattacgc ccctcgcgcc gaacgcgcgc ctgctcgcct tcgacgacaa
      301 ggccgaggcg ggcgcctatg tcgcggacca gatcgccgag gcgctgcaac aggcgctcga
      361 cgaacgcggc cgggcggtgt ggatcggctg cggcggaacg acgccgaaac cggtttatga
      421 acgcctgaac gaggccgtgc tggactggtc gcaggtcacg ctggcgcagg tcgacgagcg
      481 ctgggtatcc accgacagcc ccgattccaa cacccgcatg atgcgcgagg cgctgacgcc
      541 cgcgctcgac gccgggctga cgctggaaag cctgatgcag gacggccacg atcagcaggc
      601 gtgcgccgac aaggccgagg ccgtcctcta cgatctcggc ggcggcaagg cgccggtctt
      661 cgacttcgcc ctgctcggca tggggccgga cggccactac gcctcgatct ttccgcagca
      721 tccggtcaac gccgtggtct atgacagccc gcgcctcgtg ctgccggtgg cggcgacgga
      781 cgggacgatc gagccgaaat tgccgcgcat caccctgacg gttccctgca tcgattccag
      841 tcgcaggatt gtgttcttca tcaccggtca ggccaagcgc gatgtgctaa actcgatgat
      901 ccgcgatacg gacccgtatc tgtcgcctat cggcgcgttt ctggcgcaat gccctgtgcc
      961 ggtcgatttt gtctggactg cttgaaatga ctgaattgca tcccgttctc cacaaggtta
     1021 cgcagcgcat catcgaacgc tcaaaggcga cgcgcgcgca gttcatggcc cgcgccgacc
     1081 gctacaaggc cagcgagccg cgccgcaaga agctgtcctg cgcctactac gcccacgtcg
     1141 tcgccgccgc ctcggacaac gacaagcttc aggccgcgct cgacgccgtg cccaatctcg
     1201 gcgtcgtcac cgcctataac gacatgctgt cggcgcacca gccgtatcac gactaccccg
     1261 aaaagatccg cgactatgcc cgaaaagcgg gcgccacctc gcaggtggcg ggcggcgtgc
     1321 cggccatgtg cgacggcgtg acgcaaggcc gtcccggcat ggagctgtcg ctgttctcgc
     1381 gcgacgtcat cgccatgggc gccgccatcg cgctcagcca cgacgccttc gacgcggcgc
     1441 tgatgctcgg catctgcgac aagatcgtgc cgggcctcgt gatcggcgcc ctgtcgttcg
     1501 cgcatctgcc cgtcatcttc gtgccgtccg ggccgatgtc gtcgggcctg cccaacccgg
     1561 aaaaggccaa gatccgcacc ctgtacgcgc tgggcgaggt cgggcgcgac gcgctgctgg
     1621 ccgccgagat ggcgtcgtac cacgcggcgg gcacctgcac cttctacggc acggccaact
     1681 ccaaccagat gctgatggag atgatgggcc tgcacgttcc gggcgcggcc ttcgtgcatc
     1741 cgtcgacgcc gctgcgcgac gccctcaccg cggccgccgt cgagcgcgcg gccaggacgg
     1801 cggaaaactc caaagacccg caccgcctcg ccgacgtgct gaccgaaaag gcgttcgtca
     1861 acggcgtggt cgggctgatg gccaccggcg gctcgaccaa ccacgcgctg cacctgcccg
     1921 ccatggcgcg cgcggcgggc gtgatcctga cctgggaaga catgaacgac ctgtcggcgg
     1981 tcgtgccgct gctggcgcgc gtctatccga acggctcggc ggacgtcaac cacttccacg
     2041 cggcgggcgg cgtcggcttc gtgatccgcg agcttctgtc ggcgggcctg ctgcatgagg
     2101 acgtcgagac ggtctggggc cacggcctcg gtctttacgc cagggagccg gtgttgcagg
     2161 acggcgcgct gatctggcgc gacctgccgc aaaacagcct cgacgaggcc gtgctgcgcc
     2221 cgatctccaa cccgttccag cccgaaggcg gcctgcgcga atgcaccggc aatctcggcc
     2281 acgccatcgt caaggtgtcg gcggtcaagc cggaacacca aatcgtcgag gcgccgtgcg
     2341 tggtgttcga cgatcaggac gatttcctcg ccgccttcaa ggcgaaagac ctgccggaag
     2401 gcgatttcat ttgcgtcgtg cgcttccagg ggccaaaggc caacgggatg ccggaactgc
     2461 attcgctgac gccgtcgctg tcgtccttcc tcgacgcggg ccgcaaggtg gcgctggtga
     2521 cggacgggcg catgtcgggc gcgtcgggca aggtgccggc gatgatccac ctgacgccgg
     2581 aagctctgga cggcggaccg atcgcgcggc tcaagaccgg cgacgtgctg cgcgtcgatt
     2641 gcgaaaaagg gactttagac tacctcggag atgccaccgg gttcgccaac agaacagacg
     2701 cggagcggcc agcagaagcc gacggcgtgg gacgggaact ttttgcgcac atgcgtcggc
     2761 aggtaggccc tgccgatcag ggcggcgcag tattttggta gggatatgac agcgcatctt
     2821 aagagcggct ccgtcaccgg cgccgcgatc cgggggctgg tcggggacat cggcggcact
     2881 aacgcccgct tcgccatcgc ggagcgcaag gacggcaaga ccagactggc gcatttcaag
     2941 tcgctggaga cggcggatca cgccgacttc tacgcggccc tgacggccta tttcgagctg
     3001 gtcggcgaaa agccgggctt cgactacgcc tgcgtggcgg tcgccggtcc ggtcaaggac
     3061 ggggcgatca agttcaccaa tctcgactgg gaggtgcgcg aggaccgcct ggcgcaggtc
     3121 gcgggcgcca agacggcgaa gctgatcaac gactacgccg cgctggccta ctgcctgccg
     3181 cacctgtcgg actcggacac caagaccatc ggcccggtca ggaccggctt cggcgacgtt
     3241 cacgccgtca tgggcgcggg cacgggtttc ggcgcctcgg tgctggtcgg cgggccttac
     3301 gggccgtact gcctgtccac cgagtcgggc cacatctcgt tcggcccggt caacgattac
     3361 gaaaccgaaa tgctgcgctt cctgcgccgc aagcacggcc gcgtcaccgt cgagatgatg
     3421 ctgtccggcc ccggcctcgt gaacctctat caggcgatcg cctcgatccg cggcgaggtc
     3481 gccgaggaac tgaccccggc ccagatcact caccttgagg gcgacgacgc gcagggctgc
     3541 cgctatacgg tcgaggcgtt cctcgacatc atggccagcg tctgcggcga tctggcgctg
     3601 gcgcacggcg ccacgtcggg catgttcatc gcgggcggca tcgcgccgcg cctgatcaag
     3661 cacatcgacg agttgcgctt ccgcgcccgc atggaggcca aggcgccgat ggccggtctg
     3721 gtggcgtcga tcccgtccag catcatcatc cacccctacg ccgccctgct cggctcggcc
     3781 aacgcgctga ccgatcatca gattttagcc tgaggccttc ctcccctgcg aagcggggga
     3841 ggcggcgagc ctgtcgagcc ggagggggtt ttctcctcgc cggtattgcc ccctccgtca
     3901 cggacgagcc gtgacacctt ccccgcttcg caggggagga taagcagatt gaggacaacg
     3961 ccatgagcac ccatcccgat atcgaaaagt tcatgaccat gggccccgtg ctgccggtca
     4021 tggtgatccc gtcgctggat caggcgctgc cgctggcgga cgcgctgatc gcgggcggca
     4081 tccgggt
//
LOCUS       DASVLL010000634         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_259609,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000634 DASVLL010000000
VERSION     DASVLL010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259609"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VF402_10415"
     CDS             complement(<1..560)
                     /locus_tag="VF402_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX7800755.1"
                     /translation="MKTLSAAALMALSLPLILPLIWPAAASAQIVALPQGAVEGVTQY
                     DVESFKGIPYAAPPVGELRWRAPQAAPAWTGVRVADRFAPDCLQHALRGDDAPLSVKP
                     SEDCLYLNVWRPADMKPGAKLPVMVWVYGGGYVSGGTSPAIYDGKAFARDGIVFVSFN
                     YRVGRFGFFAFPELTKEDADHGQLGD"
     gene            726..923
                     /locus_tag="VF402_10420"
     CDS             726..923
                     /locus_tag="VF402_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX7800756.1"
                     /translation="MNNDRIQGSWDQVRGNVQKMWGKLTDDDLNVIEGDRKILAGKIQ
                     ERYGIAKDEAEKQIDEWNRTH"
     gene            complement(1031..1180)
                     /locus_tag="VF402_10425"
     CDS             complement(1031..1180)
                     /locus_tag="VF402_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800757.1"
                     /translation="MSRPRIYDEPDIEDLLAEPIVRRLMARDGVNPDDVRALMRRVMR
                     DAAQK"
     gene            1278..1757
                     /locus_tag="VF402_10430"
     CDS             1278..1757
                     /locus_tag="VF402_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800758.1"
                     /translation="MTPDAANRPPNLCDLRSLRYAEHFCIWAIRTSVACSPQCRTLLR
                     EFESAFGSRAADGMSAWRALMEALAGAARRLRIGRPGLIQLTHDETSLARMLAAAQAH
                     ERDLFMAHARWIMGHERLDDLYDEARRFTGLLRERGHICALPEVCAGAAAERGLIAL"
     gene            complement(1743..1946)
                     /locus_tag="VF402_10435"
     CDS             complement(1743..1946)
                     /locus_tag="VF402_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800759.1"
                     /translation="MNWSKAIRTTHRWLSIVFTVAALINIAAVILWVQAMWISLLAAI
                     PLIPMLITGLYMFFLPYLTSKRD"
     gene            complement(1943..2095)
                     /locus_tag="VF402_10440"
     CDS             complement(1943..2095)
                     /locus_tag="VF402_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7800760.1"
                     /translation="MVTFTLTPTADGARLRMEQSGFRPDQKQAFGGARIGWAQFFDKL
                     DKVAAQ"
BASE COUNT          381 a          682 c          682 g          360 t
ORIGIN      
        1 tagtcgccga gctggccgtg atcggcgtcc tccttggtca gttccgggaa ggcgaagaag
       61 ccgaagcgcc cgacgcggta gttgaagctg acgaaaacaa tgccgtcgcg ggcgaacgcc
      121 ttgccgtcat agatggcggg cgaggttccg cccgacacgt agccgccgcc atagacccag
      181 accatgaccg gcagcttcgc gcccggcttc atgtcggcgg gacgccagac attgaggtag
      241 aggcagtctt ccgacggctt gacgctgagc ggcgcgtcgt cgccgcgcag cgcatgttgc
      301 agacagtccg gcgcgaaacg gtcggcgacg cgcacgcccg tccaggccgg cgccgcctgc
      361 ggcgcgcgcc agcgcagctc cccgaccggc ggcgcggcgt aagggatgcc cttgaagctc
      421 tcgacatcgt actgcgtcac gccctccacc gcgccctgcg gcagggcgac gatctgcgcc
      481 gaggcggcgg cgggccaaat caggggcaag atcaggggca ggctcagggc cataagggcg
      541 gcggcgctca gggtcttcat ctcggtttcc tccgtttcgc atgaccctag catgaaggcc
      601 ggaccggtca aaccggcgga tttatacgct tcttatctgg cggcgcgctt agggcttgga
      661 aaatttagtc atgacggggc tagactgttt ttctatgaaa ttcaaagagt tttaaggagg
      721 ttatcatgaa caacgaccgt atccagggca gctgggatca ggtgcgcggc aatgtgcaga
      781 agatgtgggg caagctgacc gacgacgacc tcaacgtcat cgaaggcgac cgcaagattc
      841 tggccggcaa gattcaggaa cgctacggca tcgccaagga cgaggccgaa aagcagatcg
      901 atgaatggaa ccgcacgcac tgatcatccg atctcatcgc attgtcggcc cggttcccgc
      961 cggaccgggt ccggcatgac gggcgggaaa gcgtctcctc agatgcgctt tcccgcccaa
     1021 ttttttggcc ctatttttgg gccgcgtcgc gcatcacgcg ccgcatcagg gcgcgcacgt
     1081 catcggggtt cacgccgtcg cgcgccatca ggcgccgcac gatcggctcg gccagaagat
     1141 cctcgatatc cggttcgtca tagattcgcg gcctgctcat aacaccctct tgcgtcctcg
     1201 gcgcttgcat cctcgctgac ggcatgttaa tgcaattgag aattattcgc aatacatccg
     1261 ccaagggaat taagcccatg actccggacg ccgccaaccg ccctccgaac ctctgcgacc
     1321 tgcgcagcct gcgctacgcc gagcatttct gcatctgggc gatccgcacc tcggtcgcct
     1381 gctcgccgca gtgccgcacc ctgctgcgcg aattcgaaag cgccttcggc tcgcgcgcgg
     1441 cggacggcat gtcggcgtgg cgcgccctga tggaggcgct ggcgggcgcc gcgcgtcggt
     1501 tgcgcatcgg gcggcccggc ctgatccagc tcacgcacga cgaaacctcg ctggcgcgca
     1561 tgctggccgc cgcgcaggcg cacgagcgcg acctgttcat ggcgcatgcg cgctggatca
     1621 tggggcatga gcggctggac gatctctatg acgaggcgcg gcgcttcacc ggcctgctgc
     1681 gcgagcgcgg ccatatctgc gccctgccgg aggtctgcgc cggggcggcg gcggagcgcg
     1741 gcctaatcgc gctttgaggt cagatacggc aggaaaaaca tgtaaagccc ggtgatcagc
     1801 atcgggatca gcggaatcgc cgccagaaga ctgatccaca tcgcctgaac ccacagaatg
     1861 acggcggcga tattgatcag cgccgccacg gtgaagacga tggacagcca gcggtgcgtc
     1921 gtgcgtatag ccttgctcca gttcattgcg cggcgacctt gtccagcttg tcgaagaact
     1981 gcgcccagcc gatcctggcg ccgccgaacg cctgcttctg atcgggccgg aagccggact
     2041 gctccatgcg caggcgcgcg ccgtccgccg tcggggtcag ggtgaaggtc accacgcttt
     2101 tcagc
//
LOCUS       DASVLL010000635         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_155104,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000635 DASVLL010000000
VERSION     DASVLL010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155104"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VF402_10445"
     CDS             complement(<1..972)
                     /locus_tag="VF402_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800761.1"
                     /translation="MTEQTSLWRRFHDGYAPWVFRGGAVFLLLFAFFAPDHIALLVFL
                     MGALLLGQIRSIGAMWAPAACFGLLLLLGFARSDFIDYVRQGMSLGAAYHKSVRYFQA
                     PMLEWVACWAAIMAAWRLPPERGAGPMRWLMWAFAALTVLQLLDTVSFMSLRNAINNQ
                     FFGGHRPEMVVVLVSNSNTVLMFLFWPMAYHLARTRNYAVLWGAAAAIVGCAFVVDTN
                     AQVLAMAASTIVFLAARFWPWRAGPVKPERVLAVLAGGAVLAFPPLVVWLMRSGLARK
                     LETDILPSWAARIDIWSFAAGKSLEKPVWGWGFESARNFAPIIPDHPH"
     gene            complement(969..>1811)
                     /locus_tag="VF402_10450"
     CDS             complement(969..>1811)
                     /locus_tag="VF402_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7800762.1"
                     /translation="IVGGGYIAVEFAGIMHGLGVDVTLLYRGQKILRGFDDEVRDHLS
                     NEMTLRGVKIHTECDPVKLHLDADGRTVVTLNTGGTLTVDKALYASGRKPKTEGLNLD
                     AIGVTPDVHGAVPVDPFSRTEVENVYAIGDVTNRANLTPVAIREAVAFVETVYKDNPT
                     AYDHENIPTAVFSQPQIGTVGLSEAEATEKGIEFDVYKTRFKPMKTSFVGGESRIFMK
                     LIVERESDVVLGAHMVGGDAGEIIQLAGIAVKAKLTKAQWDATCAVHPTAAEELVTLK
                     DKRA"
BASE COUNT          338 a          639 c          555 g          279 t
ORIGIN      
        1 gtgcggatgg tcgggaatga tcggcgcgaa attgcgcgcg ctctcgaacc cccagcccca
       61 gaccggcttt tccagcgact tgccggcggc gaagctccag atgtcgatgc gcgccgccca
      121 cgacggcagg atgtccgtct caagcttgcg cgccagtccc gaccgcatca gccacacgac
      181 cagcggcgga aaggccagca ccgcgcctcc ggccagcacg gccagcacgc gctccggctt
      241 caccggcccc gcgcgccacg gccagaagcg cgccgccagg aacacgatgg tcgaggccgc
      301 catcgccagc acctgcgcat tggtgtcgac cacgaaggcg cagccgacaa tggccgccgc
      361 cgcgccccac agcaccgcat agttgcgcgt gcgggccaga tgatacgcca tcggccagaa
      421 caggaacatc agcacggtgt tggagttgga caccagcacc acgaccatct ccgggcggtg
      481 gccgccgaag aactggttgt tgatcgcgtt acgcaggctc atgaacgaca cggtgtcgag
      541 caattgcagc accgtcagcg ccgcaaacgc ccacatcagc caccgcatcg gtcccgcgcc
      601 gcgctccggc ggcaaacgcc acgccgccat gatcgccgcc cagcacgcga cccattccag
      661 catcggcgcc tgaaaataac gcaccgactt atgataggcg gcgcccaggc tcatgccctg
      721 ccgcacatag tcgatgaagt ccgagcgcgc gaagccgagc agcagcaaaa ggccgaaaca
      781 cgccgccggc gcccacatgg cgccgatgga gcgtatctgc cccagcagca gcgcgcccat
      841 caggaagacc agcagcgcga tatgatcggg ggcgaaaaag gcgaacagca gcaggaaaac
      901 cgcgccgccg cggaacaccc acggcgcgta gccgtcatga aaacgccgcc agaggctcgt
      961 ctgttccgtc atgcgcgctt atccttcaag gtcaccagtt cttccgccgc ggtcggatgc
     1021 acggcgcagg tcgcgtccca ctgcgccttg gtcagcttcg ccttgacggc gattccggcc
     1081 agttgaatga tctcgcccgc atcgccgccg accatgtggg cgccgaggac aacatcgctt
     1141 tcccgttcga cgatcagctt catgaagatg cggctctcgc cacccacgaa gctggtcttc
     1201 atcggcttga agcgcgtctt ataaacgtcg aattcgatcc ccttctccgt cgcctcggcc
     1261 tcgctcaggc cgaccgtgcc gatctgcggc tgcgaaaaga cggcggtggg gatgttctcg
     1321 tgatcgtagg cggtcggatt gtccttgtag accgtctcga cgaaggcgac cgcctcgcgg
     1381 atcgccaccg gggtcaggtt ggcgcgattg gtgacgtcgc cgatggcgta cacgttctca
     1441 acctcggtgc gcgagaacgg atcgaccggc acggcgccgt ggacgtccgg cgtgacgccg
     1501 atggcgtcga ggttcagccc ctcggtcttg ggcttgcggc ccgaagcgta cagcgccttg
     1561 tccaccgtca gcgtcccgcc ggtgttgagc gtgaccaccg tgcgcccgtc cgcgtcgaga
     1621 tgcagcttca ccggatcgca ttcggtgtga atcttcacgc cgcgcagcgt catctcgttg
     1681 ctgagatggt cgcgcacctc gtcgtcgaaa ccgcgcagga tcttctggcc gcgatagagc
     1741 aaggtcacgt cgacgcccag cccgtgcatg atcccggcga actcgaccgc gatatagccg
     1801 ccgccgacga t
//
LOCUS       DASVLL010000636         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_419994,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000636 DASVLL010000000
VERSION     DASVLL010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419994"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VF402_10455"
     CDS             complement(<1..214)
                     /locus_tag="VF402_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800763.1"
                     /translation="MGKDVAERKPGPINYWAWGSGDVVGAGLPAVTSGWLIYFYTTFC
                     HIDALKAGGLLAIPRIFDAITCPLIGY"
     gene            complement(249..1412)
                     /locus_tag="VF402_10460"
     CDS             complement(249..1412)
                     /locus_tag="VF402_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7800764.1"
                     /translation="MTNQILKPLHPERLPELLRHQTAGLDADRDDYLGAVNRLIDNLV
                     HIEDETGQFLLRLEDGRVIDTKGWHGFEWTQGVGLYGIYKHWEMTGDPKAWRIMIQWF
                     EDRFKEGMPSRNINTVAPFLTLAYLYERTGDRRYLPYLDVWGEWVYNDLPRTEEGGFQ
                     HIVYNSVNNQQMWDDTLMMSVLPLAKIGLLLNRPHYVEEAKRQFLIHIKYLADRKTGL
                     WFHGWTFDGRHNFANALWGRGNSWVTIVIPEFIELLDLPKDDALRVFLIDTLEAQVKA
                     LAKVQAPSGLWRTILDDETSYEEAAASAGFAYGILKAVRKGYIPKAYEAIGVKGAKAV
                     RAHISKDGELTQVSFGTPVFNTIQEYKDIPLTSMPFGQAMALLAMGEFMYRFI"
     gene            complement(1418..>2650)
                     /locus_tag="VF402_10465"
     CDS             complement(1418..>2650)
                     /locus_tag="VF402_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HEX7800765.1"
                     /translation="REAAIDGAALALTGDTSRDSTLDIWAPQGVAAVAFNGQALTTHA
                     NAYGGLTAAAVPGPASVTPPDLMTIRWERRSGSPEADPNFDDSGWRKADLATSNATAA
                     TQPPAGQPVLDMSQYGFHHGDVWYRGRFTVGAKSTIDSSDFFYGGGAAGLMQVWIDGQ
                     FVGQDEIPGGDPRPKGTARAHFDLNKLTPGAHEISVMVRNNAHNWDLAADDEHKEARG
                     LISASLSQKGGAPFAVPIDWKIQGDKGGEHIADLVRGPMNNGGLYGERMGWYLPARRS
                     DAGWTAAAPTDAPPNAGTYWLRARVPLDFPKDQDVQLGLQIGDPDTPQSTHKTRALIF
                     VNGWHVGNFAANIGPQHVFVLPQGIIDPDGDNTLTLAVTTDGQPQNALEPVKLVVLHN
                     MRGGVPLDIVPSPSSMQR"
BASE COUNT          450 a          795 c          895 g          510 t
ORIGIN      
        1 tatagccgat caaaggacag gtgatggcgt cgaagatgcg cggaatggcc agcaggccgc
       61 ccgccttcag cgcgtcgata tggcagaagg tggtgtagaa atagatcagc cagccggagg
      121 tgacggcggg caggcccgcg ccgaccacgt cgccggagcc ccaggcccag taattgattg
      181 gccccggctt gcgttccgcc acatccttac ccatgatttt tctcttttaa tactgtaatc
      241 taatttgttc agatgaaccg atacatgaat tcgcccatgg ccagaagcgc catggcctga
      301 ccgaagggca tcgaggtcag cggaatgtcc ttgtattcct gaatcgtgtt gaacaccggc
      361 gtgccgaacg acacctgcgt caattctccg tctttggaaa tatgcgcgcg cacggccttg
      421 gcgcccttga cgccgatggc ttcgtaagct ttcgggatat agcccttgcg cacggctttc
      481 aggatgccgt aggcgaagcc cgccgaggcg gcggcttcct cgtaggaggt ctcatcgtcg
      541 agaatggtgc gccacaggcc ggacggggcc tgtactttcg ccagcgcctt gacctgcgct
      601 tccagcgtgt cgatcaggaa gacgcgcagg gcgtcgtcct tcggcaggtc cagaagctcg
      661 atgaattccg ggatcacgat ggtgacccac gaattgccgc ggccccacag cgcattggcg
      721 aaattgtgcc gtccgtcgaa ggtccagccg tggaaccaca ggccggtctt gcggtcggcc
      781 aggtatttga tgtggatcag gaactggcgc ttggcctcct cgacatagtg gggacggttg
      841 agcaaaagac cgatcttggc cagcggcagc accgacatca tcagcgtgtc gtcccacatc
      901 tgctgattgt tgacgctgtt atagacgatg tgctggaagc cgccttcctc ggtgcgcggc
      961 aggtcgttat agacccactc gccccagaca tcgagatagg gcaggtagcg gcggtcgccg
     1021 gtgcgctcat agagataggc cagggtcagg aagggcgcga ccgtgttgat gttgcgcgac
     1081 ggcatgcctt ccttgaagcg gtcctcgaac cattggatca tgatccgcca cgccttggga
     1141 tcgccggtca tctcccagtg cttgtagatg ccgtagaggc cgacgccttg cgtccattcg
     1201 aagccgtgcc agcccttggt gtcgatcacc cggccgtctt cgaggcgcag caggaactgg
     1261 cccgtctcgt cctcgatatg gaccagattg tcgatcaggc ggttgacggc gccgagatag
     1321 tcgtcgcggt cggcgtccag ccccgccgtc tggtggcgca gcagttcggg caggcgttcg
     1381 ggatggagcg gtttcaggat ctggttggtc ataaggctta gcgttgcatg ctggagggag
     1441 aggggacgat atcgagcgga accccgccgc gcatattgtg aagcacgacg agtttgaccg
     1501 gttcaagcgc gttttgcggc tggccgtccg tggtcacggc cagggtgagc gtgttgtcgc
     1561 cgtcggggtc gatgatcccc tgcggcagga cgaagacatg ttgcgggccg atattggcgg
     1621 cgaaattgcc gacatgccag ccgttgacga agatcagggc gcgcgtctta tgggtcgatt
     1681 gcggcgtatc gggatcgccg atctgcaatc ccaattgcac gtcctgatcc ttaggaaaat
     1741 cgagcggaac ccgcgcgcgc agccagtagg ttccggcgtt cggcggcgcg tcggtcggcg
     1801 cggcggcggt ccagcccgcg tcggagcgcc tggcgggcag ataccagccc atgcgctcgc
     1861 cgtagaggcc gccattgttc atcggaccgc gcacgaggtc ggcgatatgc tcgccgccct
     1921 tatcgccctg aatcttccag tcgatgggca cggcgaacgg cgcgccgcct ttttgcgaca
     1981 aggaggcgct gatcaggccg cgcgcctcct tatgctcgtc gtcggcggcc agatcccagt
     2041 tatgggcgtt gttgcgcacc atgaccgaga tttcatgcgc gccgggcgtc agcttgttga
     2101 ggtcgaaatg cgccctggcc gtgcccttgg ggcgcggatc gccgccgggg atttcgtcct
     2161 gaccgacgaa ctggccgtcg atccagacct gcatcagacc cgccgcgccg ccgccgtaga
     2221 agaaatcgct tgaatcaatc gttgatttcg cgccgaccgt gaagcgtccg cgataccaga
     2281 catcgccgtg gtggaagccg tactggctca tgtccagcac gggctgaccg gcgggcggct
     2341 gcgtggcggc ggtggcgttg gaggtcgcga ggtccgcctt gcgccagccg gaatcgtcaa
     2401 aattcgggtc ggcttcgggc gagccggagc gccgctccca gcggatcgtc atcaggtccg
     2461 gcggcgtgac ggacgcgggg ccggggacgg cggcggcggt gaggccgcca taggcgttgg
     2521 cgtgcgtcgt cagggcctga ccgttgaacg cgaccgccgc gacgccttgc ggcgcccaga
     2581 tgtcgagcgt cgagtcccgt gacgtatcgc cggtcagggc cagcgcggcg ccgtcgatgg
     2641 cggcttcgcg
//
LOCUS       DASVLL010000637         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_98387, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000637 DASVLL010000000
VERSION     DASVLL010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98387"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="cyoA"
                     /locus_tag="VF402_10470"
     CDS             <1..849
                     /gene="cyoA"
                     /locus_tag="VF402_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEX7800766.1"
                     /translation="GGCSRSLFDPAGPVGADDATILIDATVIMLAIIVPTILLAFWMA
                     WRYRASNTKAEYLPHWSYSGRIEAVVWSIPILTIIFLGGVIWIGSYKLDPFKPLASAT
                     PPLEVQVVSLDWKWLFIYPKQGVATVNQLVVPAGRPVHFSITSASVFNVFFIPRLGSM
                     VYAMPGMVSQLNLQADRPAVLFGQSSMFSGDGFSDMQFETRSVTPAAFDSWIAAARGS
                     GTALDRPAYAALARQSQQVAPAIYAKVDPGLFQAIATQAIAPAPGPEPENASHAGRGV
                     TNGGSH"
     gene            846..2864
                     /locus_tag="VF402_10475"
     CDS             846..2864
                     /locus_tag="VF402_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7800767.1"
                     /translation="MIMFGKLNWSAIPFSQPIPIVTSIVLMLIIIGLLGLITVKGWWP
                     YLWREWITSVDHKRVGIMYCLLGVVMLIRGFADAIMMRTQQAVAIHAPGYLPPEHYDQ
                     IFSAHGTIMIFFAAMPLVIGFMNFVTPLQLGVRDVAFPTLNSVSFWLTASGALLVNIS
                     LFIGEFARTGWLPYPPLSETAYSPGVGVDYYLWAVQISGVGTLMTGINFVTTVLKMRC
                     PGMSLFRMPMFCWTTLAASLLIVAVFPILTATLAMLSLDRIFGWHYFTSGAGGNPMMF
                     MNLIWAWGHPEVYILVLPAFGIFSEVFSTFSGKPLFGYRSMVAATLFICIVAMMTWLH
                     HFFTMGSGPAVNTAFGISTSIIAVGTGVKIYNWIFTMYGGRVRLSVPMLWSLGFIFTF
                     VIGGMTGVLLAVPPADFLVHNSMFLVAHFHNVIVGGVVFGLFAGLDYWFPKAFGFRLH
                     EGWGKAAFWFSIVGFWVTFAPLYILGLYGMTRRLQHINMESWAPLLWLSEAGIVILIL
                     GAASQVTMLVVSILDRDKLRDTSGDPWDGRSLEWATPSPPPFFNFAVLPDVEGEEAYW
                     GVKLKAIESQQLAPEPQYAAIEMPVNSPVGFFTAAFASVTRFALIWHIWWLAGLGLAA
                     AFAGFVVFAWRDVHEFHVPAEEVAEAERRRRAAREALLEKLAAEGALS"
     gene            2861..3553
                     /locus_tag="VF402_10480"
     CDS             2861..3553
                     /locus_tag="VF402_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7800768.1"
                     /translation="MSDSSLPAAVAALRRLREDPHRLGHSGHGEGPAEGIGSGGTGPA
                     TKRVIVGYGFWLFLLSDIVMFACFFATFAVQRGATAGGPGLPQIVEVPRVAWETAALL
                     LSSYTCGLSFAATNARSLLWTQVFLLITGLLGLAFVGMELQEFLSLFARGITPQRSGM
                     LSAFFGLVGLHGLHVTLAGLWLLTMMAQVQVKGFRREIVHRLICFNLFWHALDIVWIG
                     VFTIVYLMGSRA"
BASE COUNT          492 a         1223 c         1133 g          715 t
ORIGIN      
        1 ggcggctgtt cgcgcagcct gttcgatccg gcggggccgg tcggcgcgga cgacgccacc
       61 atcctgatcg acgccaccgt gatcatgctg gcgatcatcg ttccgaccat attgctcgcc
      121 ttctggatgg cgtggcgcta ccgcgcctcc aatacgaaag ccgaatacct gccgcactgg
      181 tcctattcgg gccggatcga ggccgtggtc tggtcgatcc cgatcctgac catcatcttt
      241 ctgggcgggg tgatctggat cggctcgtac aagctcgacc cgttcaagcc gctggcctcg
      301 gccacgccgc cgctggaggt gcaggtggtg tcgctcgact ggaaatggct gttcatctat
      361 ccgaagcaag gcgtggccac cgtcaaccaa ctggtcgttc cggcgggcag gccggtgcat
      421 ttctccatca cctcggccag cgtgttcaac gtcttcttca tcccgcgcct cggctcgatg
      481 gtctatgcga tgccgggcat ggtgtcgcag ctcaaccttc aggcggaccg gcccgccgtc
      541 ctgttcggcc agtcctcgat gttcagcggc gacggctttt cggacatgca gttcgagacg
      601 cgcagcgtta cgcccgccgc cttcgacagc tggatcgccg ccgcgcgcgg atcggggacg
      661 gcgctcgacc ggcccgccta cgcggcgctg gcgcgtcaga gccagcaggt cgcgccagcg
      721 atctacgcca aggtcgatcc cggcctgttc caggccatcg cgacgcaggc catcgcgccc
      781 gcgcccggac cggagcctga gaacgcgtcg cacgcgggcc gcggagtcac aaacggcggg
      841 agtcactgat catgttcggc aagctcaact ggtccgccat tccgttcagc cagccgatcc
      901 cgatcgtcac ctcgatcgtg ctgatgctga tcatcatcgg cctgctgggc ctgatcaccg
      961 tcaagggctg gtggccgtat ctgtggcgcg aatggatcac ctcggtcgat cacaagcgcg
     1021 tcgggatcat gtactgcctg ctcggcgtcg tgatgctgat ccggggcttc gccgacgcga
     1081 tcatgatgcg cacccagcag gcggtggcga tccacgcgcc cggctacctg ccgccggaac
     1141 attacgacca gatcttctcg gcgcacggca cgatcatgat cttcttcgcc gccatgccgc
     1201 tggtgatcgg cttcatgaac ttcgtcacgc cgctgcaact gggcgtgcgc gacgtggcgt
     1261 ttccgacgct gaactcggtc agcttctggc tgacggcctc gggcgcgctg ctggtcaaca
     1321 tctccttgtt catcggggaa ttcgcgcgca ccggctggct gccctatccg ccgctcagcg
     1381 agacggccta ttcgcccggc gtcggcgtcg actactatct ctgggcggtg cagatttccg
     1441 gcgtcggcac gctgatgacc gggatcaact tcgtcaccac cgtgctgaag atgcgctgtc
     1501 cgggcatgag cctgttccgc atgccgatgt tctgctggac cacgctggcc gccagcctgc
     1561 tgatcgtggc ggtgtttccg atcctgaccg cgactctggc catgctcagc ctcgaccgca
     1621 tcttcggctg gcattatttc accagcggcg cgggcggcaa tccgatgatg ttcatgaacc
     1681 tgatctgggc gtggggccac ccggaggtct atatcctggt cctgcccgcc ttcggcatct
     1741 tttccgaagt gttctcgacc ttttccggca agcctttgtt cggctaccgc tcgatggtgg
     1801 cggcgaccct gttcatctgc atcgtcgcca tgatgacgtg gctgcaccac ttcttcacca
     1861 tggggtcggg tccggcggtc aacaccgcct tcggcatctc gaccagcatc atcgcggtcg
     1921 gcaccggcgt gaagatctac aactggatct tcaccatgta cggcgggcgc gtgcggctta
     1981 gcgtgccgat gctgtggtcg ctgggcttca tcttcacctt cgtgatcggc ggcatgaccg
     2041 gcgtgctgct ggcggtgccg cccgccgact tcctggtgca caactcgatg tttctggtcg
     2101 cgcacttcca caacgtcatc gtcggcggcg tggtgttcgg cctgttcgcc gggctcgact
     2161 actggttccc caaggcgttc ggcttccgcc tgcacgaagg ctggggcaag gcggccttct
     2221 ggttctcgat cgtcggcttc tgggtcacct tcgcgccgct ctacatcctg ggcctctacg
     2281 gcatgacgcg gcggttgcag cacatcaaca tggagtcgtg ggcgccgctg ctgtggctgt
     2341 ccgaggccgg gatcgtgatc ctgatcctcg gcgcggccag tcaggtcacc atgctggtgg
     2401 tcagcatcct cgatcgcgac aagctgcgcg acacaagcgg cgatccgtgg gacgggcgct
     2461 cgctggaatg ggcgacgccc tcgccgccgc ccttcttcaa cttcgccgtc ctgcccgatg
     2521 tcgagggcga ggaagcctat tggggcgtca agctgaaagc catcgagagc cagcaactgg
     2581 cgcccgaacc gcaatacgcg gccatcgaaa tgccggtcaa cagcccggtc ggcttcttca
     2641 ccgccgcctt cgccagcgtc acgcgcttcg cgctgatctg gcacatctgg tggctggcgg
     2701 gcttaggtct ggccgccgcc ttcgcgggct tcgtcgtctt cgcctggcgc gacgtgcatg
     2761 agttccatgt gccggccgaa gaggtcgccg aggccgagcg caggcgccgc gccgcgcgcg
     2821 aggcgctgct ggagaaactg gccgcggaag gggcgctgtc atgagcgatt cgtccctgcc
     2881 cgccgccgtg gccgccctgc gccgcctgcg cgaggatccg caccggctcg gccacagcgg
     2941 ccacggcgaa ggccccgccg agggcatagg ctccggcggg accggcccgg cgaccaagcg
     3001 cgtcatcgtc ggctacggct tctggctgtt cctgctcagc gacatcgtga tgttcgcctg
     3061 cttcttcgcg accttcgccg tgcagcgcgg cgccacggcg ggcggccccg gcctgccgca
     3121 gatcgtggag gttccgcgcg tggcgtggga aaccgccgcg cttttgctgt ccagctacac
     3181 ctgcggcctg tccttcgccg ccaccaacgc gcgcagcctt ctgtggacgc aggtcttcct
     3241 gctgatcacc ggccttctgg gcctcgcctt cgtcggcatg gagctgcagg agttcctgtc
     3301 cctgttcgcg cgcgggatca cgccgcagcg cagcggcatg ttgtccgcct tcttcggcct
     3361 cgtcggcctg cacggcctgc acgtcaccct ggccggtctg tggctgctga ccatgatggc
     3421 gcaggtgcag gtcaagggct tccgccgcga gatcgttcac cgcctgatct gcttcaacct
     3481 gttctggcac gccctcgaca tcgtctggat cggcgtgttc acgatcgttt acctgatggg
     3541 gagccgcgca tgagcgaccg cga
//
LOCUS       DASVLL010000638         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_259996,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000638 DASVLL010000000
VERSION     DASVLL010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259996"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF402_10485"
     CDS             complement(<1..253)
                     /locus_tag="VF402_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7800769.1"
                     /translation="MSVSPSFRDFAPRVDPVQGKTVLITGGTGSFGKRMVQTLLARFD
                     PKKIIIFSRDELKQYEMRCDLEEGLDPQQRARLRFFLGDV"
     gene            complement(303..1094)
                     /locus_tag="VF402_10490"
     CDS             complement(303..1094)
                     /locus_tag="VF402_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX7800770.1"
                     /translation="MDIPCRLSLLGAEVDAVTPACVLDFIAARVAQGRGGVVANHNLH
                     SLYLYRKSARMRDFYRRADLIEIDSTPMIAWGRLLGYDLSRANRCTYLDFREDFWTRA
                     QAGDWRVYHIGGAREHNAASKAAILQRHPRARLDVHTGYFDMDGPDNEALLADLHARR
                     PQVLLVGMGMPRQEMWILNNLDRLPPCVILPVGAAFDYEAGVMYAAPRWTGRLGVEWL
                     VRFAHEPRRLFSRYFLEPWHLVPQALGDLFRARRAARPTPALTVN"
     gene            complement(1167..1826)
                     /locus_tag="VF402_10495"
     CDS             complement(1167..1826)
                     /locus_tag="VF402_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7800771.1"
                     /translation="MRKLVLGLTAIAFVLAGCETTDTSNGLRTRSNTQTGALIGAAAG
                     AAAGALSNRKADQARRNAVIGAGIGALAGAGVGAYMDRQNKDLRARLEGHGVYVTRRG
                     DDIVLNMPGDITFASGSADLSASFMPVLNDVGTILNQYPSTYIDIVGYADSQGSDAYN
                     LDLSNRRANAVAGYLVGQKVKAERIYVAGMGEAHPIASNDTPEGRARNRRVEITLRPV
                     E"
     gene            complement(1968..3698)
                     /locus_tag="VF402_10500"
     CDS             complement(1968..3698)
                     /locus_tag="VF402_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX7800772.1"
                     /translation="MLVGAVMARAQDADADSSSGSLTDMVEAGLQPVNSTKALKTAEQ
                     SVVRVLVVYRGFGGQPLDTVGMGSGFVVAPGYIVTNYHVVEAPPEASSADIYIVPHKD
                     TGAGYQQVKLVKPWIEGDLALLAAPNLKIDPLKLFLTPYKNERVVAMGYPDVTDHLLN
                     RSGTALLEPADAYVTQGSIALFASSNPDGSRVDTLFHTAPINHGNSGGPLLNECGQVV
                     GVNTWTAPSTVSAGGDLDVAAGQFVATHVSALNTFLASAGIKPQIVSTPCYAKTEDEI
                     IKDDALTKALTAAASAQAQRLDEQRKAESDRAIMERLQLGAMVVLSLLVLVLIALIVR
                     REMRHRAELHAHREPDAPTAFSDEAEPATGKAKPVKAEKLRVEKPLRVPRPRLRHPIP
                     WGWILLGLIIVIAVAGFLIKDRDIYKRLTQPKTPVPVALDTAVHMVCQVNAGASPHPL
                     SGAGPVEFEFDARHACVNGRTPYERQTDGTLVRFTLGDSDPVAARMELSADGAVFRRY
                     DYRLDPAAYKTFLAQRVALGSLRCSSHDDAGALAALSDNLARVRLLSQSYLTVPPENQ
                     TVWACHRADS"
BASE COUNT          607 a         1223 c         1386 g          618 t
ORIGIN      
        1 gcacgtcgcc gaggaagaag cgcaggcgcg cgcgctgctg cggatcgagg ccctcttcga
       61 ggtcgcagcg catctcgtac tgcttcagct cgtcgcgcga gaagatgatg atcttcttcg
      121 gatcgaagcg cgccagcagg gtctgcacca tgcgcttgcc gaacgaaccg gtgccgccgg
      181 tgatcagcac ggtcttgccc tgaacgggat cgacgcgcgg cgcgaaatcg cggaaggacg
      241 ggcttacgga catggcggcc tgacgggagg ttgtctcagg cggcgagtgt cgggcggcgc
      301 gcttaattca cggttaatgc gggcgtcgga cgcgcggcgc ggcgcgcgcg gaaaaggtcg
      361 cccagcgcct gcgggaccag atgccacggc tccaggaaat agcggctgaa caggcggcgc
      421 ggctcgtgcg cgaaccgcac cagccattcc acgcccaacc ggccggtcca gcgcggcgcg
      481 gcgtacatca cgcccgcctc gtagtcgaag gcggcgccca ccggcaggat cacgcacggc
      541 ggcagccggt cgagattgtt caatatccac atttcctgac gcggcatgcc catgccgacc
      601 agcagcacct gcggccgtct ggcgtgaaga tcggccagca gcgcctcgtt gtcggggccg
      661 tccatgtcga aatagccggt atgcacgtcc agccgcgcgc gcggatggcg ttgcaggatc
      721 gccgccttcg acgcggcgtt gtgttcgcgc gcgccgccga tatgatagac gcgccagtcc
      781 ccggcctgcg cgcgcgtcca gaagtcctcg cggaagtcga gataggtgca gcggttggcg
      841 cggctcagat cgtagcccag cagcctgccc caggcgatca tcggcgtcga atcgatctcg
      901 atcaggtcgg cgcggcggta gaagtcgcgc atccgcgcgc ttttgcggta caggtagagg
      961 ctgtgcagat tgtgattggc caccacgccg ccgcgccctt gcgcgacgcg cgcggcgatg
     1021 aagtcgagga cgcaggccgg ggtgacggcg tcgacctcgg cgcccagaag ggaaaggcgg
     1081 cagggaatat ccatgccgcc caccttaacg ccgccggttt gacattccct caaaagacag
     1141 ggtgaatccg gcgtttacct caggcttcat tccaccgggc gcagggtgat ttccacgcgg
     1201 cggttcctgg cgcggccttc gggcgtgtcg ttgctggcga tcgggtgggc ctcgcccatg
     1261 cccgcgacat agatgcgctc ggccttgacc ttctggccga ccagataacc ggccacggcg
     1321 ttggcgcggc ggttggacag gtcgagatta taggcgtccg agccctggct gtcggcgtag
     1381 ccgacgatgt cgatataggt cgacggatat tgattcagga tcgtcccgac gtcgttcagc
     1441 accggcatga aggaggcgct gaggtcggcg ctgcccgagg cgaaggtgat gtcgccgggc
     1501 atgttcagca cgatgtcgtc gccgcgccgg gtgacataga cgccgtggcc ttccagcctg
     1561 gcgcgcagat ccttgttctg gcggtccata taggcgccga cgcccgcccc ggccagagcg
     1621 ccgatgcccg cgccgatgac ggcgttgcgg cgcgcctgat cggccttccg gttgctgagc
     1681 gcgcccgccg ccgcgcccgc cgccgcgccg atcagggcgc cggtctgggt gttggagcgg
     1741 gtccgcagcc cgttcgacgt gtcggtggtt tcgcagcccg ccaggacgaa ggcgatggcg
     1801 gtcaggccga gtacgagttt acgcatggtg gtttcctcag atgtaaatcc tttttagcgc
     1861 catactggca cagcggacgt aagaaacagg gccggaaccg gcgcgcggcg catcagaatc
     1921 cgataagcgc ggcctgtaac ggggaaccgc gcgctcagga aagcgaatca ggaatcggcg
     1981 cgatggcagg cccagaccgt ctggttttcg ggcggcacgg tcaggtagct ttgcgacagg
     2041 agccgcacgc gcgccagatt gtcgctgagc gcggccagcg cgcccgcgtc gtcgtgcgag
     2101 gagcagcgca gcgaccccag cgccacgcgc tgcgccagaa aggtcttgta ggccgccgga
     2161 tcgagccggt aatcgtagcg gcggaacacc gcgccgtcgg cggacagctc catgcgcgcg
     2221 gccaccgggt cggaatcgcc gagcgtgaag cggaccagcg tcccgtccgt ctggcgctca
     2281 tagggggtgc ggccgttgac gcaggcgtgg cgggcgtcga attcgaactc gaccggcccg
     2341 gcgccgctga gcggatgcgg cgaggcgccc gcattgacct gacacaccat atgcaccgcc
     2401 gtgtccagcg ccacgggaac cggcgtcttg ggctgggtca ggcgcttgta gatgtcgcgg
     2461 tccttgatca ggaagcccgc cacggcgatc acgatgatca ggccgagcag gatccagccc
     2521 cacgggatcg gatggcgcag gcgcgggcgc ggcacgcgca gcggcttttc gacgcgcagc
     2581 ttctcggcct tgacgggttt ggccttgccc gtggcgggtt cggcctcgtc cgagaaggcg
     2641 gtcggcgcgt cgggttcgcg atgggcgtgc agttcggcgc ggtggcgcat ttcgcgccgc
     2701 acgatcagcg cgatcagcac cagcaccagc agcgacagca cgaccatggc gcccagttgc
     2761 aggcgctcca tgatggcgcg gtcggattcg gccttgcgct gctcgtcgag gcgctgcgcc
     2821 tgcgccgagg cggcggcggt cagcgccttg gtcagggcgt cgtccttgat gatctcgtcc
     2881 tcggtcttgg cgtagcacgg ggtcgagacg atctgcggct tgatgcccgc cgaggcgagg
     2941 aaggtgttga gcgccgagac gtgggtggcg acgaactggc ccgccgcgac gtcgagatcg
     3001 ccgcccgccg agacggtgga cggcgcggtc caggtgttga cgccgaccac ctggccgcat
     3061 tcgttgagca gcggcccgcc ggaattgccg tggttgatcg gcgcggtgtg gaacagggtg
     3121 tcgacgcgcg agccgtccgg gttggacgag gcgaacagcg cgatcgagcc ttgcgtgaca
     3181 taggcgtcgg cgggctccag cagggcggtg ccggaccggt tgagcaggtg gtcggtgacg
     3241 tcgggatagc ccatcgccac gacgcgctcg ttcttgtacg gcgtcaggaa cagtttgagc
     3301 gggtcgatct tgaggttcgg cgcggccagc agggccaggt cgccctcgat ccacggcttg
     3361 accagcttga cctgctgata gccggcgccg gtgtccttgt gcgggacgat gtagatgtcg
     3421 gcggacgagg cttcgggcgg cgcctcgacg acgtggtagt tggtgacgat gtagccgggc
     3481 gcgacgacga agccggagcc catgccgacc gtgtcgagcg gctggccgcc gaagccgcga
     3541 tagacgacca gcacgcgcac caccgactgc tcggcggttt tcagcgcctt ggtcgagttg
     3601 accggctgca gccccgcctc gaccatgtcg gtcagcgagc cgctggacga atcggcgtcg
     3661 gcgtcctgcg cgcgcgccat gacggcgccg accagcataa gacaggccag aaggcaggtc
     3721 gcggcggtaa gccagacccg ttgagaatgt tgcgacttgc ccgccccgat tcgatgcacg
     3781 attttcgttc cgcgccggtc catccggcgt ttgcgctaca tagcggagat cgta
//
LOCUS       DASVLL010000639         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_314142,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000639 DASVLL010000000
VERSION     DASVLL010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314142"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VF402_10505"
     CDS             <1..586
                     /locus_tag="VF402_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7800773.1"
                     /translation="PHYVEEAKRQFLIHIKYLADRKTGLWFHGWTFDGRHNFAGALWG
                     RGNSWVTIVIPEFIELLDLPRDDALRVFLIDTLEAQVKALAKVQAPSGLWRTILDDET
                     SYEEAAASAGFAYGILKAVRKGYISKAYEAIGVAGAKAVRAHISSEGELTQVSFGTPV
                     FNTIQEYKDIPLTSMPFGQAMALLAMGEFMYRFI"
     gene            632..2185
                     /locus_tag="VF402_10510"
     CDS             632..2185
                     /locus_tag="VF402_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800774.1"
                     /translation="MQERKTGQVNYWGWGAGDLVGAGITAVTSGWMIYFYTAFCGLSA
                     AEAGSLFAISRFFDAFTCPLIGYISDKLRHTWVGRHIGRRKIFLLIAIPLLPSFALMW
                     VSGQSYLYYLLTYLFFELMYNFVLIPWETLPAEMSKDYKVKAKFAGARILCAQASAIL
                     ASGLPALIINHLGKDSAATFLINGAIFGALASLVIIIAVITTWERPYTEDEKRIQPSP
                     FNAAEALLLPVQIFRDLLSTLRIRAFRQHLSIYLGGYISQDIFNAAFAIFVAVVMMGS
                     TLIISQMMTVMYFVQLLSVAIAIRVVMSAGPTRAYAAAITAFIVACVLYYVFYIAPPA
                     GFAAAVAGIEHDIFGGAFKVAPAVAFWLFAPVIFAGLGRGTLNFVPWSVYNYIPDVDE
                     AVTGERREGIFAGVMTLVRKLSQAVAIMAATAAIDAGGFVKGGGQQTPEAIHTVVLVL
                     VVGPIVVMLLGLIVALRFRLNAKTHAVLMDEIVRLRDGATEPATPESGRVVEDLTGWK
                     YAQLWGRGR"
     gene            complement(2182..2490)
                     /locus_tag="VF402_10515"
     CDS             complement(2182..2490)
                     /locus_tag="VF402_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7800775.1"
                     /translation="MTYPVIFAPEAFAQLDALEAYIAAFDAPMAAKRFVDEIVDYCLR
                     FDQFPMRGVAREDLRPGLRLVGYRRRATIAFSVKPDRVDILGVFYGGQNIEAAFGADD
                     "
BASE COUNT          441 a          845 c          783 g          481 t
ORIGIN      
        1 tccccactat gtcgaggagg ccaagcgcca gttcctgatc cacatcaaat acctggccga
       61 ccgcaagacc ggcctgtggt tccacggctg gaccttcgac ggacggcaca atttcgcggg
      121 cgcgctgtgg gggcgcggca attcgtgggt caccatcgtg atcccggaat tcatcgagct
      181 tctggacctg cccagggacg acgcgctgcg cgttttcctg atcgacacgc tggaagcgca
      241 ggtcaaggcc ctcgcaaaag tgcaggcccc gtccggcctg tggcgcacca ttctcgacga
      301 cgaaacctcg tatgaggaag ccgccgcctc ggcgggcttc gcctacggca tcctgaaggc
      361 cgtgcgcaag ggctatatct ccaaggctta cgaggccatc ggcgttgcgg gcgccaaggc
      421 cgtgcgcgcc cacatttcca gcgaaggcga actgacgcag gtgtccttcg gcacgccggt
      481 gttcaacacg attcaggaat acaaggacat tccgctgacc tcgatgccct tcggtcaggc
      541 catggcgctt ctggccatgg gcgaattcat gtatcggttc atctgatttc gccagatagc
      601 cgatttataa aagaaagttt ggaggcgtaa ggtgcaggaa cgtaagacgg gacaggtcaa
      661 ttactggggc tggggcgcgg gcgatctcgt cggcgcgggc atcaccgctg tcacgtcggg
      721 gtggatgatc tatttctaca ccgccttctg cggcctgagc gcggcggagg cgggcagcct
      781 gttcgccatt tcgcgtttct tcgacgcctt cacctgcccg ctgatcggct atatttccga
      841 caagctgcgc cacacctggg tcgggcggca tatcgggcgg cgcaagatct tcctgctgat
      901 cgccattccg ctcctgccct cgttcgcgct gatgtgggtg tcgggccaga gctatctcta
      961 ctatctgctg acctacctgt tcttcgagct gatgtacaat ttcgtgctga ttccgtggga
     1021 gaccctgccc gccgaaatga gcaaggacta caaggtcaag gccaagttcg ccggcgcgcg
     1081 catcctgtgc gcccaggcgt cggccatcct ggcctccggc ctgcccgcgc tgatcatcaa
     1141 ccatctgggc aaggattcgg cggcgacctt cctgatcaac ggcgcgatct tcggcgccct
     1201 ggcctcgctg gtcatcatca tcgccgtgat caccacctgg gagcggccct ataccgagga
     1261 cgaaaagcgc atccagccct cgccgttcaa cgccgccgag gcgctgctgc tgccggtgca
     1321 gattttcagg gatctgctct ccaccttgcg catccgcgcc ttccgccagc acctgagcat
     1381 ctatctgggc ggctatatct cgcaggacat cttcaacgcc gccttcgcca tcttcgtcgc
     1441 cgtggtgatg atgggatcga ccctgatcat ctcgcagatg atgacggtca tgtacttcgt
     1501 gcaactgctg tcggtcgcca tcgccatccg cgtggtgatg agcgccgggc cgacgcgcgc
     1561 ctacgccgcc gccatcaccg ccttcatcgt cgcctgcgtg ctgtactacg tgttctacat
     1621 cgcgccgccc gccgggttcg ccgccgccgt cgcgggcatc gaacacgaca tcttcggcgg
     1681 cgcgttcaag gtcgcgcccg ccgtcgcctt ctggctgttc gcgccggtca ttttcgccgg
     1741 gctgggacgc ggcacgctga acttcgtgcc gtggagcgtc tacaactaca ttcccgacgt
     1801 ggacgaagcc gtgacgggcg agcgccgcga aggcatcttc gcgggcgtga tgacgctggt
     1861 gcgcaagctg tcgcaggccg tcgccatcat ggccgccacc gccgccatcg acgcgggcgg
     1921 cttcgtcaag ggcggcggcc agcagacgcc cgaagccatc cacacggtcg tgctggtgct
     1981 ggtcgtcggg ccgatcgtgg tcatgctgct gggcctgatc gtagcgctgc gcttccgcct
     2041 caacgccaag acccacgccg tgctgatgga cgagatcgtc cgcctgcgcg acggcgccac
     2101 ggaaccggcc acgccggaaa gcggcagggt ggtcgaggat ctgaccggct ggaaatacgc
     2161 gcaactgtgg ggacgcggac gctaatcatc cgcgccgaac gccgcctcta tattctgacc
     2221 gccgtagaag acgccgagaa tatcgacccg atccggcttc accgagaagg cgatcgtcgc
     2281 cctgcggcgg tagccgacca gacgcagccc cggacgcagg tcttcacgcg ccacgccacg
     2341 catgggaaac tgatcgaacc ggaggcaata atcgacaatc tcgtcgacaa accttttcgc
     2401 cgccattggt gcgtcgaagg cggcgatata ggcttccaga gcgtcgagct gagcgaaagc
     2461 ttccggcgcg aaaatgactg gataggtcaa acgttgcgcg ccgcgcgacg ttccgccaac
     2521 cgggcgcgca cctcatcggg cgtcaggccg
//
LOCUS       DASVLL010000640         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_155567,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000640 DASVLL010000000
VERSION     DASVLL010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155567"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VF402_10520"
     CDS             <1..347
                     /locus_tag="VF402_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7800776.1"
                     /translation="WAERLNAMPKYVLSSTLKTPEWSNATVLSGDLSGEIMKLTSRID
                     GDIVVIGSLQLVQALWTHDLVDELRLKVFPVTLGGGRRLFPENGEMKNMRRTAAAALG
                     DGLSYLIYERAR"
     gene            426..2243
                     /locus_tag="VF402_10525"
     CDS             426..2243
                     /locus_tag="VF402_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7800777.1"
                     /translation="MAKAPAPLLALKAVRLMDGATPLFDGVDIALEPRMRACLVGRNG
                     AGKSTLMRMLSGLIEPDSGERYVQQGLRFAFVLQEPEPSGATVRDWVCSGGAESGGVE
                     PYAADAELAAFGIDPDTSCASMSGGEKRRAALAKAFAEAPDLLFLDEPTNHLDIFAIE
                     TLEDRLRSFKGASLIVSHDRTFLERTTKTCFWLYDRRVLRRDEGYVGFEDWQAKIEAE
                     TAESLRRLEKAIERETYTFYRSITAQRTRNEGRAARLRAMRADHAERMKTMDKPMEMT
                     IDAGPTSGKLVAELKNVSKAFGDKTILKNFSTRILRGDRLAIVGPNGAGKSTLVKLLI
                     GQIGADSGDIRLGTNLDVAYLDQGRDALKGDETLWDVLANSGSDSILVRGQPRHVAAY
                     AKDFLFREKQLRQPVKSLSGGERNRLQLARALAKATNLLVLDEPTNDLDMDTLDLLED
                     MLADYEGTLILVSHDRDFIDRLATSTLALDGKGRAAETPGGWQDFIRQNPDFFADISQ
                     KEARKIAEKPAAAPPPKTAPKKLSYKDQKRLDDLNALMPKWSVEIKRLEAVLDDPALY
                     SRDPKRFDATMKALDAVRAELENGEMEWLELEEKRESLG"
BASE COUNT          469 a          870 c          777 g          386 t
ORIGIN      
        1 cctgggccga gcgcctgaac gccatgccca aatatgtgct gtcctcgacc ctgaagacgc
       61 ctgagtggtc caatgcgacg gttttgagcg gcgacctgag cggcgaaatc atgaagctga
      121 catcacgcat cgacggcgac atcgtcgtga tcggcagcct ccagttggtt caggccctgt
      181 ggacgcacga tctggtcgat gaattgcggc tgaaggtctt tccggtgacg ctcggcggcg
      241 ggcggcgcct gttccccgaa aacggtgaga tgaaaaacat gcgccgcacg gcggcggcgg
      301 cgctgggcga cggcctgtcc tacctgattt acgaacgggc gcggtaggcc ccgcatcaaa
      361 gcggcaggga agcgcggaca gaaaaaccct ttgccattcc ggcctcgcgc cactacatcc
      421 ccggcatggc caaagccccc gctcccctgc tcgccctgaa agccgtccgt ttgatggacg
      481 gcgccacgcc tttgttcgac ggcgtcgata tcgcgctgga gccgcgcatg cgcgcctgtc
      541 tggtcgggcg caacggcgcg ggcaagtcga cgctgatgcg catgttgtcc ggcctgatcg
      601 agccggattc cggcgagcgc tacgtccagc agggtctgcg tttcgccttc gtgttgcagg
      661 agccggagcc aagcggggcc acggtgcgcg actgggtctg ttctggcggt gctgaatctg
      721 gcggggtcga gccttacgcc gccgacgccg agttggccgc cttcggcatc gacccggata
      781 cgtcctgcgc ctccatgtcc ggcggcgaaa agcgccgcgc ggcgctggcc aaggcgttcg
      841 ccgaagcgcc cgacctcctg ttcctcgacg agccgaccaa ccacctcgac atcttcgcca
      901 tcgagacgct ggaagaccgt ctgcggtcgt tcaagggcgc ctcgctgatc gtcagccacg
      961 accgcacctt tcttgagcgc acgacgaaga cctgcttctg gctctacgac cgccgcgtcc
     1021 tgcgccgcga cgaaggctat gtcggcttcg aggactggca ggcgaagatc gaggccgaaa
     1081 ccgccgaatc cctgcgccgt ctcgaaaagg ccatcgaacg cgaaacctac accttctacc
     1141 gttcgatcac cgcccagcgc acgcgcaacg aaggccgcgc cgcgcgcctg cgcgccatgc
     1201 gcgccgacca cgccgaacgc atgaagacga tggacaagcc gatggagatg accatcgatg
     1261 cgggtccaac ctccggcaag ctggtcgccg aactgaaaaa cgtctccaag gcgttcggcg
     1321 acaagacgat actgaaaaac ttctcgaccc gcatcctgcg cggcgaccgg ctggccatcg
     1381 tcggcccgaa cggcgcgggc aagtccaccc tggtcaagct gctgatcggc cagatcggcg
     1441 cggacagcgg cgacatccgc ctcggcacga acctcgacgt cgcctatctc gatcagggcc
     1501 gcgacgcgct caagggcgat gagaccctgt gggacgtgct ggccaattcc ggttcggatt
     1561 cgatcctcgt gcgcggccag ccgcgccacg tcgccgccta cgccaaggac ttcctgttcc
     1621 gcgaaaagca gttgcgccag ccggtcaagt cgctgtcggg cggcgagcgc aaccgcctgc
     1681 aactggcgcg cgccctggcc aaggcgacca acctgctggt gctggacgaa ccgaccaacg
     1741 atctcgacat ggacacgctg gacctgctgg aggacatgct ggccgactat gagggcacgc
     1801 tgatcctcgt ctcgcacgac cgcgacttca tcgaccggct ggcgacctcg accctcgcgc
     1861 tcgacggcaa gggccgggcc gccgaaaccc ccggcggctg gcaggacttc atccgccaga
     1921 acccggactt cttcgccgac atctcgcaaa aggaagcgcg caagatcgcc gaaaagcccg
     1981 ccgccgcgcc gccgcccaaa accgcgccga aaaagctctc ctacaaggac cagaagcgcc
     2041 tcgacgacct gaacgcgctg atgccgaaat ggtccgtcga gatcaaacgg ctggaggccg
     2101 tcctcgacga tcccgcgctc tacagccgcg atccgaaacg cttcgacgcc acgatgaagg
     2161 cgctcgacgc cgtgcgcgcc gaactggaaa acggcgaaat ggagtggctg gagcttgagg
     2221 aaaagcgcga aagccttggc tgatccttac ccagggcgcg ccctaaccaa aatttaaatc
     2281 cattgttcta cggttctccg gttgcaactg gaaagaccgc ccggagccat gcccttctgc
     2341 gccaaagcct tgcgaatctg cgtcccggtc ctgaccgcgg cggcgatgac ggcaggcggc
     2401 gcgtccgcag cgatgcccta tctcgactgg cgcgacaagg cggccgacgc gaccgctccg
     2461 gcggcctatc ccgtgccgcc gtccccctat ggccaggtcg gc
//
LOCUS       DASVLL010000641         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_420472,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000641 DASVLL010000000
VERSION     DASVLL010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420472"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /gene="tsaD"
                     /locus_tag="VF402_10530"
     CDS             <1..907
                     /gene="tsaD"
                     /locus_tag="VF402_10530"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7800778.1"
                     /translation="EAGNPELDAVAATAGPGLIGGVMVGLSFAKGFALARGLPLIGVN
                     HLEGHALSARLAEDVAFPFLLLLVSGGHCQLLSVRGVGDYERLGTTIDDAAGEAFDKT
                     AKVLDLGYPGGPALEQCAKDGDPARFRLPRARAGHKDCDFSFSGLKTAAAQIAHAEVR
                     TRQDRADLCAGVQDAIARQLVERSERAMTLFRQATDAARPAFVVAGGVAANATVRRVL
                     QAAAERHGFSFTAPPMAYCTDNAAMIALAGLEHFARACDKPEAVADLRAKAMGVAARP
                     RWPLDEARALAQPVHKTAGRKGAKA"
     gene            924..>1682
                     /locus_tag="VF402_10535"
     CDS             924..>1682
                     /locus_tag="VF402_10535"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7800779.1"
                     /translation="MAEIYHKVGVIGAGAWGTALSQVLTLGGNAVLLHAREPEVAESI
                     RSVRENSLYLPGVTLSEAIAPTTEIADLAGCDLILAVPPAQHMRATLSAFAPHMRAGT
                     PVVLCAKGVERGTDALMTEVLAAVLPGAVQAVLAGPNFAREVAMGLPSAVTLACKDEA
                     LGAKIAATLAGPTFRPYQSTDLIGAESGGAIKNVIAIACGISEGKGLGRNAHAALITR
                     GFAEMTRLAIAMGAQLATMAGLCGMGDLVLTCSSP"
BASE COUNT          238 a          557 c          644 g          243 t
ORIGIN      
        1 cgaggcgggg aacccggagc tggacgccgt ggcggcgacg gcggggccgg ggctgatcgg
       61 cggggtgatg gtcgggctgt ccttcgccaa gggattcgcg ctggcgcgcg gcctgccgct
      121 gatcggggtc aaccatctgg aagggcatgc gctgtcggcg cggctggccg aagatgtggc
      181 gtttccgttc ctgctgctgc tggtttcggg cgggcattgc cagcttctga gcgtgcgcgg
      241 cgtcggcgat tacgagcgcc tcggcaccac catcgacgac gcggcgggcg aagccttcga
      301 caagacagcc aaggtgctgg atctcggcta tccgggcggc ccggcgctgg aacaatgcgc
      361 gaaggatggc gatccggcgc gtttcaggct gccgcgcgcg cgcgccgggc ataaggattg
      421 cgacttctcg ttttcgggcc tgaagacggc ggcggcgcag atcgcccatg ccgaggtcag
      481 gacgcgccag gaccgcgccg acctgtgcgc gggcgtgcag gacgccatcg cgcggcaact
      541 ggtcgagcgc tccgagcgcg cgatgacgct gttccggcag gcgacggacg cggcccgtcc
      601 ggccttcgtc gtggcgggcg gggtcgcggc caacgccacg gtgcggcggg ttctgcaagc
      661 ggcggcggag cgtcacggct ttagcttcac cgcgccgccg atggcctatt gcaccgataa
      721 cgccgcgatg atcgcgctgg cgggccttga acatttcgcg cgggcctgcg acaaacccga
      781 agccgtggcc gatctgcgcg cgaaggcgat gggcgtcgcc gcccgtccgc gctggccgct
      841 ggacgaggcg cgcgctctgg cgcaaccggt gcacaagacc gccggacgta agggcgccaa
      901 ggcgtaaact caagggagcg gatatggcgg agatttatca caaggtgggc gtgatcggcg
      961 cgggcgcgtg gggcacggcc ctgtcgcagg tgctgaccct gggcggcaat gcggtgctgt
     1021 tgcacgcgcg cgagccggag gtcgcggagt cgatccgcag tgtgcgcgag aacagcctct
     1081 acctgcccgg cgtgaccctg tccgaagcca tcgcgccgac gaccgagatc gccgatctgg
     1141 ccggatgcga cctgattctg gccgtgccgc ccgcccagca catgcgcgcc accttaagcg
     1201 ccttcgcgcc gcacatgcgc gccggaacgc cggtcgtgct gtgcgccaag ggggtcgagc
     1261 gcggcacgga cgcgctgatg accgaggtgc tggccgccgt gctgcccggc gcggttcagg
     1321 ccgtgctggc cgggccgaac ttcgcgcgcg aggtggcgat ggggctgccg tcggcggtga
     1381 ccctggcctg caaggacgag gcgctgggcg cgaagatcgc cgccaccctg gccggaccca
     1441 ccttccgccc gtaccagtcc accgacctga tcggcgccga atcgggcggg gcgatcaaga
     1501 acgtcatcgc catcgcctgc ggcatcagcg agggcaaggg actgggccgc aacgcccacg
     1561 ccgccctgat cacgcgcggc ttcgcggaga tgacgcggct ggccatcgcg atgggcgcgc
     1621 aactggcgac catggcgggg ttgtgcggca tgggcgatct ggtgctgacc tgctcgtcgc
     1681 cg
//
LOCUS       DASVLL010000642         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_366628,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000642 DASVLL010000000
VERSION     DASVLL010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366628"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VF402_10540"
     CDS             complement(<1..233)
                     /locus_tag="VF402_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEX7800780.1"
                     /translation="MYRLATRALHVLQPEDAHSLTILLLKAGLGPASSLSTPELSVDL
                     AYDGGSLRFPNCVGLAAGFDKNADVPLAMIRAG"
     gene            complement(226..723)
                     /locus_tag="VF402_10545"
     CDS             complement(226..723)
                     /locus_tag="VF402_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7800781.1"
                     /translation="MKQLVIMRHAKAEKAPAGGEDFDRPLSGDGRDEAASVARALAAY
                     GVKPDFALVSSARRTLETFRQVEGVLGEIPALIGKDLYNAGADALRRAVEAHADDGRC
                     LLLVAHNPGVQYLVADYLFEGAASPDIVGKVQNGYPTATATVFEVDVAGRPVYDGIYL
                     AKDHV"
     gene            complement(766..1527)
                     /locus_tag="VF402_10550"
     CDS             complement(766..1527)
                     /locus_tag="VF402_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7800782.1"
                     /translation="MRRTVEDLNRFYASPEGATVRRLIGNKLAEAWPDVRGLDVLGVG
                     YCTPYLDSCVEPRRVLSAMPGGQGAEIWPADARVRSVLVEEEALPFPSALFDRILMMH
                     ALEEAPSPQGLLLEASRLLSANGRLIIGVAARGGFWAHAERTPFGYGQPYSRMQLEAA
                     LRDAELEPLAWSYALYAPPWSVVRRWAAPLERVLPALWPISGGLILMEAARRPFVART
                     RTAQKSLLKDIQGALAPVPAPGALPTPTSRELEQA"
     gene            1603..2340
                     /gene="gloB"
                     /locus_tag="VF402_10555"
     CDS             1603..2340
                     /gene="gloB"
                     /locus_tag="VF402_10555"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX7800783.1"
                     /translation="MTLEIEMFPALQDNYGFLITDAASGRTAMIDAPEPAAMLRVLDG
                     RRLDFILNTHWHPDHAGGNALIKARTGCVIYGPDEVRRIAPLDHEIKPGDAFRLGGTD
                     LQVMDLGGHTLGHIGYYSAADGAAFVGDTLFPLGCGRLFEGTPRQMWASLARIAALPP
                     ETALYSAHEYTLASLKFAESLGGDDALRARGERVRRLRAEGRATVPATVAEELATNPF
                     LVWPLRENGDAARAAKFAELRAAKDRF"
     gene            complement(2337..>2786)
                     /locus_tag="VF402_10560"
     CDS             complement(2337..>2786)
                     /locus_tag="VF402_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4908 domain-containing protein"
                     /protein_id="HEX7800784.1"
                     /translation="LTLFTDDRPMGDPASVNGKAEAFIPGHMSPTALFQTLVRASRRV
                     TLAVGRNFGFDAPDVTPGADYIYADAAQVTADALSHIANQPRGRRALDPVHGVQFVEG
                     RPPSATLSNGVLILKLDIERGLWGGHVSSKRITNVVMASYGVADAGR"
BASE COUNT          451 a          934 c          995 g          406 t
ORIGIN      
        1 aacccggcgc ggatcatggc cagcggaacg tcggcgttct tgtcgaatcc ggcggccagc
       61 ccgacgcagt tgggaaagcg taagctcccg ccgtcatagg ccagatcgac gctcagttcc
      121 ggggtggata gcgacgacgc cggcccaaga ccggccttga gcagcaggat cgtcagactg
      181 tgcgcgtcct ccggctgaag aacgtgcagc gcgcgcgtgg ccaatctata catgatcttt
      241 cgccaagtag attccgtcat agaccgggcg gcccgcgaca tcgacctcga acacggtcgc
      301 cgtggcggtc ggatagccgt tctgaacctt gcccacgatg tccggcgaag cggcgccctc
      361 gaacagatag tccgcgacca gatattgcac gccgggatta tgcgcgacca gcagcaggca
      421 tcggccgtcg tccgcatgcg cctcgacggc gcggcgcagg gcgtcggcgc ccgcattata
      481 aaggtctttg ccgatcaggg ccggaatctc gcccagcacg ccctcgacct gccggaaggt
      541 ctccagcgtg cgccgcgccg acgacaccag cgcgaagtcc ggtttgacgc cataggcggc
      601 cagagcgcgc gcgaccgaag ccgcctcgtc gcggccgtcg ccgctgagcg ggcggtcgaa
      661 atcctcgccc ccggcaggcg ccttttccgc cttggcgtgg cgcatgatga caagctgttt
      721 catgaccctt cctatggcgc cgaatcggcg cgtgggcaat cgggcttacg cctgttccaa
      781 ctcgcgtgag gtcggggtcg gcagcgcgcc gggcgccgga accggggcca gcgcgccctg
      841 aatgtctttc agcagcgatt tctgcgccgt gcgcgtgcgc gcgacgaacg gcctgcgcgc
      901 cgcctccatc aggatcaggc cgccgctgat cggccacagg gcgggcagga cgcgctccag
      961 cggcgcggcc cagcggcgca ccacgctcca cggcggcgcg tacagggcgt aggaccaggc
     1021 cagcggctcc agctcggcgt cgcgcagggc ggcctcgagc tgcatccggc tgtagggctg
     1081 gccatagccg aacggcgtgc gctcggcatg cgcccaaaag ccgccgcgcg ccgccacgcc
     1141 gatgatcagc cgcccgttgg ccgacaacag ccgcgacgcc tccagcaaca ggccctgcgg
     1201 cgacggcgcc tcctccagcg cgtgcatcat caggatgcgg tcgaacaggg cggacgggaa
     1261 cggcagcgcc tcctcctcga ccagcaccga gcgcaccctg gcgtcggcgg gccagatctc
     1321 ggcgccctgc ccgcccggca tggccgacag cacgcggcgc ggctcgacgc aactatcgag
     1381 atagggcgtg caatagccga cccccagaac gtccagcccg cgcacgtccg gccacgcctc
     1441 ggccagcttg ttgccgatca ggcggcgcac cgtcgcgcct tcgggcgaag cgtagaagcg
     1501 gttgaggtct tcgacagtgc ggcgcaaggc gcgtcctttg cggtggcgtg acgcctctct
     1561 atatagggga cggcgccgct tttggcagga ggaaatgtgg acatgacgct ggaaatcgag
     1621 atgttccccg ccttgcagga caattacggc ttcctgatca ccgacgcggc cagcggccgg
     1681 accgccatga tcgacgcgcc ggaaccggcg gccatgctgc gggttctgga cgggcggcgg
     1741 ctggatttca tcctcaacac gcattggcat cccgaccatg cgggcggcaa cgcgctgatc
     1801 aaggcgcgca cgggctgcgt catctatggg ccggacgagg tcaggcggat cgcgccgctc
     1861 gatcatgaga tcaagcccgg cgacgcgttc cggctgggcg ggacggactt gcaggtcatg
     1921 gacctgggcg ggcatacgct cggccatatc ggctattaca gcgcggcgga cggcgcagcc
     1981 ttcgtcgggg acacgctgtt tccgctcggc tgcgggcgtt tgttcgaggg gacgccgcgg
     2041 cagatgtggg cgtcgctggc gcgcatcgcg gccttgccgc cggagacggc gctttacagc
     2101 gcgcacgaat atacgctggc cagcctgaaa ttcgctgaaa gcctcggcgg agacgatgcg
     2161 ctcagggcgc gcggcgaacg ggtgcggcgg ctgcgggccg aagggcgggc gacggttccg
     2221 gcgacggtgg cggaggaact ggcgaccaat ccgttcctgg tctggccgct gcgggaaaac
     2281 ggagacgcgg cgcgggcggc gaagttcgca gagctgcgcg cggcgaagga ccgcttttag
     2341 cggcccgcat cggccacgcc gtaggaggcc atgaccacat tggtgatgcg cttggacgaa
     2401 acatggccgc cccacagacc gcgctcgatg tcgagcttga ggatcagcac gccgttgctg
     2461 agcgtggcgc tgggcgggcg gccttcgacg aattgcacgc catgcaccgg atcgagcgcc
     2521 cggcggccgc gcggctggtt ggcgatgtgg ctcagggcgt cggcggtcac ctgcgcggcg
     2581 tcggcgtaga tatagtcggc gcccggcgtc acgtccggcg cgtcgaagcc gaaattgcgc
     2641 ccgacggcca gggtgacgcg gcggctggcg cgcaccaagg tctgaaacag ggcggtgggc
     2701 gacatatggc cggggatgaa ggcctcggcc ttgccgttga ccgaggccgg atcgcccatc
     2761 ggccggtcgt cggtgaacag ggtcag
//
LOCUS       DASVLL010000643         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_475383,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000643 DASVLL010000000
VERSION     DASVLL010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475383"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VF402_10565"
     CDS             <1..1140
                     /locus_tag="VF402_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800785.1"
                     /translation="LAHGEKTARGWSFPSDIDWATDKGSGHGVDVAAHDFWAAYTWTG
                     DDKYLRPMDGALRGDLGGLTNLNADVLDRVPGEAAVAQRIAAGADKAGGGAIDRNLGG
                     ATAGQFARFVRWQETGDDAILADLYGDEVEAVAQRMPVLTSAELWVDRVSVPSDLLQR
                     TRLGGVAHRRMAFYPGNLVSWRFSSADHEVSGENVAILIRKGDPRAFSVTAYNLTDQP
                     VTAHMTGAQLAAGLWTLTGPDGTRDVRLERGRGVDLALPPRRTVSFDLTLKTPGDDPA
                     TRPDIGISSRDLILNGRTLSITAHSLGARPTPAGVAVVTDAAGHELTRVRFGPLPAPT
                     DLTPKTVYIEARLPKTSGPLRVTLRLDGQPDEITQINNSAVTPGA"
     gene            1167..2741
                     /locus_tag="VF402_10570"
     CDS             1167..2741
                     /locus_tag="VF402_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7800786.1"
                     /translation="MRRIIQFGMASALALATGFAALIPASYGQDAQTSTSASSSSASE
                     NTVAAGQEPQTVTIFGKKRDQGDPTITQIPTHDADSCGFMNNYDPANEEIVQDYLKSF
                     YGDYTSTVGGGATGDSSGADQNGVRFKDNSPFGNVSEDNAADHNTLPGMQIGGTDASG
                     APTDGANSNCLPSDKAFAAGRSYIARTDHSLKDAFDAFDARDYPKALDLFGKSYAKMG
                     YDSAALMEGKMYLAGMGTRADAQQAVVWLKKVVEARFGPADVLTFNPDDPTYMNTRID
                     AAMTLAKIYLIGQAGVARDPAEARRWYMKADDFGYMPATHTVGLMYENGYAGEKNLPK
                     AAGYFKKAGTAGYTPSMYELGELYRAGGDGVAEDDRLGAQWLMEAAKRGHPDALYAIG
                     NMYDLGETLPHDPQKAAVYYKEAALKGQADAQDAIGLMFYTGEVVGKDQETARKWFYQ
                     AALQRQPDAMFNLGAMYANGQGGDKDLVKAYVWLKLAALKGADQATGALAAVEAHMSA
                     DDKAKADAIFHPVGNG"
     gene            complement(2756..>2977)
                     /locus_tag="VF402_10575"
     CDS             complement(2756..>2977)
                     /locus_tag="VF402_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800787.1"
                     /translation="AAVGCGFFGAAGVWLRAGAAHATASAAQDRRENAEDRRVMGLPV
                     TCVYDRIRSITRETGQSVAKSDEIQTYFA"
BASE COUNT          553 a          977 c         1014 g          433 t
ORIGIN      
        1 ctggcgcacg gggaaaagac cgcgcgcggc tggagcttcc cgtccgatat cgactgggcg
       61 acggacaagg ggtcggggca tggcgtcgat gtcgccgcgc acgacttctg ggcggcctat
      121 acgtggacgg gcgacgataa atatctgcgg ccgatggacg gcgcgctcag gggcgatctg
      181 ggcgggctga ccaatctcaa cgccgacgtg ctggaccggg ttccgggcga ggcggcggtg
      241 gcgcaaagga tcgcggcggg cgcggacaag gcgggcggcg gggccatcga ccgcaatctg
      301 ggcggcgcca cggcggggca gttcgcgcgc ttcgtgcgct ggcaagagac gggcgacgac
      361 gccatcctcg ccgacctcta cggcgacgag gtcgaggcgg tcgcgcagcg catgccggtg
      421 ctgacctcgg cggagctgtg ggtcgaccgc gtcagcgtgc cgagcgacct tttgcaacgc
      481 acgcggctgg gcggggtcgc gcaccggcgc atggccttct atccgggcaa tctggtcagt
      541 tggcgattct cgtcggcgga ccatgaggtg tccggcgaaa acgtcgccat cctgatccgc
      601 aagggcgatc ccagggcgtt ttccgtcacc gcctataacc tgaccgatca gccggtgacg
      661 gcgcatatga ccggcgcgca actggccgcc gggctgtgga cgctgaccgg cccggacggg
      721 acgcgcgacg tgcggctgga gcgcgggcgc ggcgtcgacc tggccttgcc gccgcgccgg
      781 acggtcagct tcgacctgac gctgaaaacg ccgggagacg atccggcgac gcggcccgat
      841 atcgggattt cgagccgcga cctgatcctg aacgggcgga ccctgagcat caccgcgcac
      901 agtctgggcg cccggccgac tccggcgggc gtggccgtcg tcacggacgc cgccggtcat
      961 gagctgacgc gcgtgcggtt cgggcctttg cccgcgccga cggacctgac gccgaaaacc
     1021 gtttacatcg aggccagact gccgaaaaca tcgggtccgt tgcgcgtcac gctgcggctg
     1081 gacggccagc cggacgagat tacgcagatc aacaacagcg ccgtaacgcc gggcgcatga
     1141 gttcacaggc gggggagacg cacatcatgc gacgcatcat ccagttcgga atggcctcgg
     1201 ccctggcgct ggcgacgggc ttcgccgccc tgattccggc cagctacggt caggacgctc
     1261 aaacttccac ttcggcctcc tcgtcgtccg cgtccgaaaa cacggtggcg gcgggacagg
     1321 agccgcagac ggtcaccatc ttcggcaaga agcgcgatca gggcgatccg accatcaccc
     1381 agattccgac ccatgacgcc gattcctgcg gcttcatgaa caattacgac cccgccaacg
     1441 aggagatcgt gcaggactat ctgaagtcct tttacggcga ctacacctcg accgtcggcg
     1501 gcggcgcgac cggcgattcc tccggcgccg accagaacgg cgtgcgtttc aaggacaatt
     1561 cgcctttcgg caacgtctcc gaggacaacg ccgccgacca caacaccctg ccggggatgc
     1621 agatcggcgg aaccgacgcc agcggcgcgc cgaccgacgg cgcgaacagc aactgcctgc
     1681 cctccgacaa ggcgttcgcg gcggggcggt cctatatcgc gcgcaccgac cattcgctga
     1741 aggacgcctt cgacgccttc gacgccaggg actaccctaa ggcgctcgac ctgttcggca
     1801 agtcctacgc caagatgggc tacgattccg ccgccctgat ggagggcaag atgtatctgg
     1861 cgggcatggg gaccagggcc gacgcgcagc aggcggtcgt ctggctgaaa aaggtcgtcg
     1921 aggcgcgctt cggcccggcg gacgtgctga ccttcaatcc cgacgatccg acctatatga
     1981 acacccgcat cgacgcggcc atgaccttgg cgaaaatcta cctgatcggg caggccgggg
     2041 tggcgcgcga tccggcggag gcgcggcgct ggtacatgaa ggccgacgat ttcggctaca
     2101 tgcccgccac gcacacggtc ggcctgatgt acgaaaacgg ctatgcgggc gagaagaacc
     2161 tgcccaaggc ggcgggttat ttcaaaaagg ccggaaccgc gggctatacg ccgtccatgt
     2221 acgaactggg cgagctgtac cgcgcgggcg gcgacggcgt ggccgaggac gacaggctgg
     2281 gcgcgcaatg gctgatggag gcggccaaac gcggccatcc cgacgcgctc tacgccatcg
     2341 gcaatatgta cgacctcggc gagacgctgc cgcacgatcc gcaaaaggcc gccgtctatt
     2401 acaaggaggc cgccctgaaa ggacaggcgg acgcgcagga cgccatcggc ctgatgttct
     2461 acaccggcga ggtcgtgggc aaggatcagg agaccgcgcg caaatggttc tatcaggccg
     2521 ccctgcaacg ccagcccgac gccatgttca acctgggggc catgtacgcc aacggacagg
     2581 gcggggacaa ggatctggtc aaggcctatg tctggctgaa actggccgcg ctgaagggcg
     2641 cggatcaggc gacgggcgcg ctggcggcgg tcgaggcgca tatgagcgcc gacgacaagg
     2701 ccaaggccga cgcgatcttc cacccggtcg ggaacggctg agccaaatat aacaattacg
     2761 caaaatatgt ctgaatttcg tcgcttttgg caacggattg tccagtttcg cgcgttatag
     2821 agcgaatacg atcataaacg cacgtaaccg gcagccccat gacccgacga tcttccgcgt
     2881 tctctctcct gtcctgcgcc gcgctggccg ttgcgtgcgc ggccccggcg cgcagccaga
     2941 cgcccgccgc gccgaaaaag ccgcagccga cggccgc
//
LOCUS       DASVLL010000644         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_366686,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000644 DASVLL010000000
VERSION     DASVLL010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366686"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1228
                     /locus_tag="VF402_10580"
     CDS             257..1228
                     /locus_tag="VF402_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7800788.1"
                     /translation="MVDRRTFLTLSAAFGAAAALPMAARAGANDLSLQDIWPNDPPGG
                     EKVTAPEQVILRHPGGDPNDTAYLHVRKPWLLLRKPKKPNGGAVLLIPGGGYVRVAVS
                     KAGGDIDAWLAGQGYTVFTMDYRLPGDPWAAGPEVALQDAQRAMRIIRSRSSELNFDP
                     ERVAIMGFSAGGHVAGLLVTRWDKPVYTPIDAIDQLSARPTTASMCYPVVTMMQPYAH
                     QQSMHAALGDDPTEAARQAASIERHVPADAPPTFVCATTNDPVVPHQNAMLMFDALNA
                     AKVPSELHLYEGATHGFPLRGKDHQLLPWGPQALAFMQRHGLDPVRA"
     gene            1231..2040
                     /locus_tag="VF402_10585"
     CDS             1231..2040
                     /locus_tag="VF402_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEX7800789.1"
                     /translation="MKTLVIAAALMAATPAFAQTPANTPPPIRVILVGDSTMTNMSGY
                     GGELCKDFTAQVACVNAARGGRSSKSYRVEGLWDKIMAQLADKKGFSKTYVLIQFGHN
                     DGSPKPERHTNIETEFPVNMRNYARDVEKAGAIPVLITPITQRHFLNGRLEPDLMPWA
                     NATRTVARDEHVTLIDLNAESMKAVQAMGLTEANTLAGQPIPQNVLDTENTGTSVPAV
                     FTDASQGQGHTQAFDYTHLGPKGQPYFARMVADLWIKADPAIAAYVKPEAR"
     gene            2101..2370
                     /locus_tag="VF402_10590"
     CDS             2101..2370
                     /locus_tag="VF402_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX7800790.1"
                     /translation="MTLNVNLTPKLEDMVRQKVSGGLYNNASEVIRDALRLMEERDRL
                     QAAKIEALRQDIQAGLTSGHAEALDIEDIRALSEKCDALFGLKSA"
     gene            complement(2441..3103)
                     /locus_tag="VF402_10595"
     CDS             complement(2441..3103)
                     /locus_tag="VF402_10595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7800791.1"
                     /translation="MITLHEPALAGLLDRLFAESDAATAALVPLMADLPDGEQARLMR
                     SKSDYIDLYGRLKAFALPVSRETGRLLYMLAHGIRARNIVEFGTSFGISTLHLAAALH
                     DNDGGRLITCEFEPSKIARARANLTEGDLIGLVEIREGDALQTLKHDLPETIDLVLLD
                     GAKALYDDVLDLLEPRLRPGAFIVADNADYCPDYLRRVRDGGGYLSVPFAEDVELSMR
                     LG"
BASE COUNT          620 a         1091 c          979 g          505 t
ORIGIN      
        1 tgaatccgcc ttccacgtcc cgcccagcgc ggtctattcc gccgtcaaac ccttcggcaa
       61 ctgatatacc ccccctctcc cctccacgag gggagaggac gagagccgag ccgtcaggcg
      121 aaggcgatcg ggtgaggggc tgtttgccga ccgggcgcag gcgtccggcc cctcacccga
      181 ggatcggcgg cttcgccttg ctcctcgtcc tctccccgca agcggggaga ggggaaaatt
      241 tataaggaga cttttcatgg ttgatcgtcg caccttcctc accctgagcg ccgctttcgg
      301 cgcggccgcg gccttgccga tggccgcgcg cgccggggcg aacgacctgt cgcttcagga
      361 catctggccg aacgatccgc cgggcggcga aaaggtcacc gcgccggagc aggtcatcct
      421 gcgccatccc ggcggcgatc ccaacgacac cgcctatctg catgtgagaa agccgtggct
      481 gctgctgcgc aagccgaaaa agccgaacgg cggcgcggtg ctcctgatcc ccggcggcgg
      541 ctatgtgcgc gtggcggtgt ccaaggcggg cggcgacatc gacgcctggc tggccggtca
      601 gggctatacg gtgttcacca tggattaccg cctgcccggc gatccgtggg cggcggggcc
      661 tgaggtcgcc ttgcaggacg cgcagcgcgc catgcgcatc atccgctcgc gcagctcgga
      721 gctgaacttc gacccggagc gcgtggcgat catgggcttt tcggcgggcg gccacgtcgc
      781 gggcctgctg gtcacgcgct gggacaagcc ggtctatacg cccatcgacg ccatcgacca
      841 gctttcggcc aggccgacca ccgcttcgat gtgctacccg gtcgtgacca tgatgcagcc
      901 ctatgcgcac cagcaatcca tgcacgcggc cctgggcgac gatcccaccg aagcagcgcg
      961 gcaggcggcc tccatcgaac ggcacgtgcc tgcggacgcg ccgccgacct tcgtgtgcgc
     1021 caccaccaac gatccggtcg tgccgcatca gaacgccatg ctgatgttcg acgcgctcaa
     1081 cgccgcgaaa gtcccgtccg aactgcacct ctacgaaggc gcgacccacg gcttccccct
     1141 gcgcggcaag gaccatcaac tgctgccgtg gggcccgcag gcgctggcct tcatgcagcg
     1201 ccacggcctc gatccggtca gggcttaatc atgaaaacgc tcgtcatcgc cgccgccctt
     1261 atggccgcaa cgcccgcctt cgcgcaaacg cccgcaaaca ccccgccgcc gatccgcgtc
     1321 atcctcgtcg gcgactcgac catgaccaat atgagcggtt acggcggcga actgtgcaag
     1381 gacttcaccg cccaggtcgc ctgcgtcaac gccgcgcgcg gcgggcgctc gtcgaaaagc
     1441 taccgcgtcg agggcctgtg ggacaagatc atggcgcaac tggccgacaa gaaaggcttt
     1501 tcaaaaacct atgtgctgat ccagttcggc cacaacgacg gttcgccgaa gcccgaacgc
     1561 cacaccaata tcgagacgga attcccggtc aacatgcgca actacgcgcg cgacgtcgaa
     1621 aaggccgggg cgatcccggt gctgatcacg ccgatcaccc agcgccattt cctgaacgga
     1681 aggctggagc ccgacctcat gccgtgggcc aacgccacgc gcaccgtggc cagggacgag
     1741 catgtcacgc tgatcgacct caacgccgag agcatgaagg cggtgcaggc gatgggcctc
     1801 accgaagcca acacattggc cggtcaaccg atcccgcaga acgtgctgga caccgaaaac
     1861 accggcacgt cggtccccgc cgtcttcacc gacgcctcgc agggtcaggg ccacacgcaa
     1921 gccttcgact acacccacct cggccccaag ggccaaccct actttgcccg catggtggcc
     1981 gatctgtgga tcaaggccga cccggcgatc gccgcctatg tgaagccgga ggcgcggtaa
     2041 ggattggcaa ggtttgacaa gtttcaaccc gccgcctaat ctggcggcgg gagacttgcc
     2101 atgacgctca atgtcaatct gacgccgaaa ctcgaagaca tggttcgcca gaaggtgtcc
     2161 ggcggcctct acaacaacgc gtccgaagtc atccgcgacg ctctgcggct gatggaggaa
     2221 cgcgaccgtc tgcaagccgc gaagatcgag gcgctgcgtc aggacattca ggccggactg
     2281 accagcggtc atgccgaagc cctcgacata gaagacatta gagcgctttc cgaaaagtgt
     2341 gacgcacttt tcggattaaa aagcgcgtaa aagcataagg ttagagcgcc gcggccgtgt
     2401 gcgttctgta tagctcgcgc gacatcgacg ctcaccgacc tcaacccagc cgcatcgaca
     2461 actcgacatc ttcggcgaac ggcacactca gatagccgcc gccgtcacgc acccgccgca
     2521 gatagtccgg gcaatagtcg gcattgtcgg cgacgatgaa cgcccccgga cgcaggcgcg
     2581 gctccagcag gtccagcaca tcgtcgtaaa gcgccttggc gccgtcgagc agcacgaggt
     2641 cgatcgtttc gggcagatcg tgtttcaggg tctgcaaggc gtcgccttcg cggatctcga
     2701 ccaggccgat caggtcgccc tcggtcaggt tggcgcgggc gcgcgcgatc ttggacggct
     2761 cgaactcgca ggtgatcagc cgcccgccgt cattgtcgtg cagggccgcg gccagatgca
     2821 gggtcgagat gccgaacgag gttccgaact cgacaatatt gcgcgccctg atgccgtgcg
     2881 ccagcatgta gagcaaccgc cccgtctcgc gcgacaccgg cagcgcaaag gccttcaacc
     2941 ggccatagag gtcgatgtaa tcgctcttgc tgcgcatcag ccgcgcctgt tcgccgtcag
     3001 gcaggtcagc catcagcggc accagcgccg ccgtcgccgc atcggattcg gcgaacaggc
     3061 ggtcgagaag cccggcaagg gcaggttcat gaagcgtgat catggaaaac tccggtaagg
     3121 atggcgcgga cttgcgccga cgacaaaaat acgaataatt attcgtgttt tctaatcgca
     3181 ttgccggacc ggatg
//
LOCUS       DASVLL010000645         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_475452,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000645 DASVLL010000000
VERSION     DASVLL010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475452"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF402_10600"
     CDS             <1..474
                     /locus_tag="VF402_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7800792.1"
                     /translation="ALRAFGMGKPSALRNRSAAAATLLHGAVADWPAGDMEACVRAVE
                     TCLLAQQPKHDPVSSKADEIVRVAQADRDIVAVSQLADAFSLSVRSIQDICNRAIGVS
                     PKWLIRCFRLQDALARLETEADMNLAALAQDLGYFDQAHFTRDFKRITGVSPSRY"
     gene            525..662
                     /locus_tag="VF402_10605"
     CDS             525..662
                     /locus_tag="VF402_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800793.1"
                     /translation="MSAIVNVIAGRNKNHTNNGENMSRRERGAMLSEPSKALALYAVL
                     H"
     gene            complement(644..937)
                     /locus_tag="VF402_10610"
     CDS             complement(644..937)
                     /locus_tag="VF402_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800794.1"
                     /translation="MIEIVGRAATATDDELLLELLLELELLGLLKPEPLPALLPPEPP
                     PPQPATSVKAVRSATPRAGAVEQILNTSPPDDIFIPFSDISNMTVWKHQCNTA"
     gene            903..2057
                     /locus_tag="VF402_10615"
     CDS             903..2057
                     /locus_tag="VF402_10615"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEX7800795.1"
                     /translation="MAVAALPTISITTDGAAPIVDKDNYVAGDMSITAPDGASAFTGR
                     LSIKGRGNSTWAMFPKKPYRIKLDSKAGLLGMPKDKNWVLLANYSDKSLLRNQVAFEI
                     SKRLGMAWTPRSVQVEVTLNGDYIGVYQLVEQVRVAADRIDITETDDTTPAVAADGGY
                     LVEINERMDDPVCWRTTRDVPLCIKDPDPSNAGQQAYIQTYIQNAEDALFSADPTQAA
                     TGYESYFDVDSLIDWYLVNELFKNVDSAGYGSIYIYKDAGGKLHYGPVWDFDLGAGNT
                     DYFDASPQGFQTATAVWIARMKAADPSFEARIRARWDAVKATQLDTLPAYIDAQAALL
                     ADAEKRNFNRWPILNTYVWPNSEVAGTYQGEVDFFRNWMTARIAWLDANL"
BASE COUNT          424 a          634 c          647 g          387 t
ORIGIN      
        1 gccttgaggg ccttcggtat gggaaaaccg tccgcgctgc gcaaccggtc ggccgccgcg
       61 gcgacgctcc tgcacggggc cgtcgccgac tggcccgccg gggatatgga ggcgtgcgtg
      121 cgggccgtcg aaacctgtct cctcgcccag cagccgaagc acgacccggt ttcatcaaag
      181 gctgacgaga ttgtgcgcgt cgcgcaggcc gaccgcgaca tcgtcgccgt ctcgcaactg
      241 gccgacgcct tttccctgtc ggtgcgctcg attcaggaca tctgcaaccg ggcgatcggc
      301 gtctcgccga aatggttgat ccgctgcttc cgccttcagg acgcgctggc gcggctcgag
      361 acggaagccg acatgaacct cgccgcgctg gcccaggatc tgggctattt cgatcaggcg
      421 cacttcacac gcgacttcaa gcggatcacc ggagtaagcc ccagccgtta ttgaccatat
      481 gagcaggttg ggccgggtgg caaagcgcgc aaaataacgg cgccatgtcc gccatcgtca
      541 acgtcatcgc aggccgaaac aaaaatcata cgaataatgg tgaaaatatg tcccggcgtg
      601 aacgcggggc tatgctgagc gaaccgtcta aggcgctggc gctctatgcg gtgttgcatt
      661 gatgtttcca aactgtcata tttgaaatat cagagaacgg tataaaaata tcgtcggggg
      721 gacttgtgtt tagaatttgc tcaacggcgc ccgcgcgcgg cgtcgcgctt ctaacggctt
      781 taacgcttgt cgccggttgc ggcggcggcg gctcaggcgg aagcagcgcg ggaagcggct
      841 ccggtttcag cagcccaagc agctccagct ccagcagcag ttccagcagc agttcgtcat
      901 cggtggcggt ggccgccctg ccgacgatct cgatcacgac ggatggcgcc gcgccgatcg
      961 tcgacaagga caactatgtc gccggcgata tgtccatcac ggcgccggac ggcgcgtcgg
     1021 cctttaccgg acgcctgtcc atcaaggggc gcggcaacag cacctgggcc atgtttccta
     1081 aaaagcccta tcgcatcaaa ctcgattcca aggcgggcct gctggggatg ccgaaggaca
     1141 agaactgggt gctgctggcc aactattccg acaagagcct gctgcggaac caggtggcgt
     1201 tcgagatcag caaacgtctg ggcatggcct ggacgccgcg ctccgttcag gtcgaggtga
     1261 cgctaaacgg ggactatatc ggcgtctatc aactggtcga gcaggtccgt gtggcggccg
     1321 accgcatcga tatcaccgag accgacgaca cgacgccggc tgtcgccgcc gacggcggct
     1381 atctcgttga aatcaatgag cgtatggacg atcccgtgtg ctggcggacg acaagggacg
     1441 tgcccttgtg catcaaggat ccggacccga gcaatgccgg tcagcaggcc tatatccaga
     1501 cctatatcca gaatgccgaa gatgcgctgt tctccgccga tccgacccag gcggcgacgg
     1561 gttatgaaag ctatttcgat gtcgacagcc tgatcgactg gtatctcgtc aatgagttat
     1621 tcaagaacgt cgattcggca ggctatggca gtatttacat ttacaaggac gcgggcggga
     1681 aactgcacta cgggccggtc tgggatttcg atctgggcgc cggcaatacg gattatttcg
     1741 acgccagtcc tcaaggcttc cagacggcga cggcggtatg gatcgcgcgt atgaaggccg
     1801 ccgacccaag cttcgaagcc cgcatccggg cgcgatggga cgccgtcaag gcgacgcagc
     1861 tcgatacctt gcccgcctat atcgacgccc aggccgcgct tctggccgat gccgaaaagc
     1921 gcaatttcaa ccgctggccg attctcaaca cctatgtctg gcccaattcg gaagtggccg
     1981 gcacgtatca gggcgaggtc gacttcttcc ggaactggat gacggcgcgc atcgcctggc
     2041 tggacgccaa cctctgataa gccggggcag caggcaatct gcaaaccttt ag
//
LOCUS       DASVLL010000646         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_420672,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000646 DASVLL010000000
VERSION     DASVLL010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420672"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VF402_10620"
     CDS             <1..462
                     /locus_tag="VF402_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7800796.1"
                     /translation="PSIREKLKTLERVGLGYIKIGQQATTLSGGEAQRVKLSKELSKR
                     ATGKTLYILDEPTTGLHFEDTRKLLEVLHELTDNGNTVVVIEHNLDVIKTADWLLDFG
                     PEGGDGGGEIVARGTPEAVADNPASWTGKYLKEMLAKHRHRQPASLTEPAE"
     gene            518..1027
                     /locus_tag="VF402_10625"
     CDS             518..1027
                     /locus_tag="VF402_10625"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HEX7800797.1"
                     /translation="MADTKHAAASLPRTAAPPRAFAGKYARFLDYYKSDPSERITMIR
                     HGVEPSDVKLLAERMKLTHKHFMQSLGLKTSTVNRKHAQQTPLTRDESERVLGLAKLI
                     GQLEKLMEESRDPDDAVDFNAAQWIGEWVQEPNMALGGKTPITFMDTSEGRDIVSNLI
                     SRLQTGAYS"
     gene            1024..1503
                     /locus_tag="VF402_10630"
     CDS             1024..1503
                     /locus_tag="VF402_10630"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX7800798.1"
                     /translation="MIVHRIATITPAYSADDLSGEGAKASGGRWNSPGAAMVYCAENR
                     ALAILETFVHVDKRNIPPMNRYFVTIDLPDDLVKTAEVLTSPPPGWDAEPAGVPSIAY
                     GDAWVAAKRSAVLVVPSVIVPDEYNYLLNPLHAQARRITARIGPKCRYDQRLFFARA"
     gene            complement(1565..>2391)
                     /gene="secF"
                     /locus_tag="VF402_10635"
     CDS             complement(1565..>2391)
                     /gene="secF"
                     /locus_tag="VF402_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7800799.1"
                     /translation="DFAGGNVIEVQTPGPAPLAELRARMPSFGASDVTVQSSGSNDVI
                     IKFKLHEGEQTEGVEAVKTRLSAAFPGVSYKRTEVVGPKVSGELLRSGLMTLGLALLL
                     MLVYIWFRFPSGLQFGAGAVIAILHDLVLTIGIMAALQIEFSMTSIAALLTVVGYSMN
                     EKVITFDRLRENLRKFKRTPLSDIINLSENERLSRTIITGSTAVLALAGMLFLGGPTL
                     FPLVFAMVFGIIVGTYSSIYVALPLILIWGVNRNDEDAEIIDMGGFKGGKKPKDLP"
BASE COUNT          489 a          790 c          717 g          395 t
ORIGIN      
        1 ccgtcgatcc gcgaaaagct caagacgctg gagcgcgtcg gcctcggcta catcaagatc
       61 ggccagcaag ccaccaccct gtccggcggc gaggcgcagc gcgtcaagct cagcaaggaa
      121 ttgtcgaaac gcgccaccgg caagacgctc tacatcctcg acgagccgac caccggcctg
      181 catttcgagg atacgcgcaa gctgctggag gtgctgcacg aactgaccga caacggcaac
      241 acggtcgtgg tgatcgagca caatctcgac gtcatcaaga ccgccgactg gctgctcgac
      301 ttcggccccg aaggcggcga cggcggcggc gaaatcgtcg cccgcggcac gcccgaagcc
      361 gtagccgaca acccggcaag ctggaccggc aaatacctga aagagatgct ggccaaacac
      421 cgccatcggc aacccgcgtc cctgacggag ccggcggaat aggcttgtcc ggagcgctca
      481 tttcgcctat aatggatgct catttggagc atccatcatg gccgatacga aacacgccgc
      541 ggcgtccctg ccccggaccg ccgcgccgcc ccgcgcgttc gccggcaagt atgcgcgttt
      601 tctcgactac tataagtcgg accccagcga acgcatcacg atgatccgtc atggcgtcga
      661 gccttcggat gtgaagctgc tggccgagcg catgaagctc acgcacaaac acttcatgca
      721 atcgctcggc ttgaaaacct ccacggtcaa tcgcaagcac gcgcagcaga cgccgctgac
      781 tcgcgacgaa tccgagcgtg tgctgggcct cgccaaactg atcgggcaac tggaaaagct
      841 gatggaggaa tcgcgcgatc ctgacgatgc ggtcgatttc aacgccgcgc aatggatcgg
      901 cgaatgggtt caggagccga acatggcgct gggcggaaag acgcccatca cgttcatgga
      961 tacgtccgaa ggccgcgaca tcgtctccaa cctgatttcg cgtctccaga ccggggccta
     1021 ttcttgatcg tccaccggat cgcgacgatc acgcccgcct acagcgccga cgacctgtcc
     1081 ggggaaggcg cgaaagcctc cggcggccgc tggaacagtc ccggcgcggc gatggtctat
     1141 tgcgccgaaa accgcgccct cgccattctg gaaaccttcg tgcatgtcga taagcggaac
     1201 attccgccta tgaaccgcta tttcgtcacg atcgatcttc ccgacgacct tgtaaaaacg
     1261 gcggaggttc tgacctcgcc gccgcccggt tgggacgccg aacccgcggg cgttcccagc
     1321 atcgcctatg gcgacgcctg ggtcgcggcg aagcgcagcg ctgttttggt cgttccgtcc
     1381 gtgatcgttc cggacgaata taattacctg ctcaatcccc tgcacgcgca ggcgcgccgg
     1441 atcaccgccc ggatcgggcc gaaatgccgc tacgatcagc ggctgttctt cgcgcgcgcc
     1501 tgaaaaacaa aaaagcgcgg aagcttaaaa cttccgcgcc ttttcatcag cttatccgca
     1561 aatcctacgg cagatccttc ggcttcttgc cgcccttgaa gccgcccatg tcgatgatct
     1621 cggcgtcctc gtcgttgcgg ttgacgcccc agatcaggat cagcggcagc gcgacgtaga
     1681 tcgacgaata ggtgccgacg atgatgccga acaccatggc gaagaccagc gggaacaggg
     1741 tcggcccgcc cagaaacagc atcccggcca gcgccagcac cgccgtcgag ccggtgatga
     1801 tggtccgcga caaccgctcg ttttccgaca ggttgatgat gtcggacagc ggcgtgcgct
     1861 tgaacttgcg caggttttcg cgcaaccggt cgaaggtgat gaccttttcg ttcatcgaat
     1921 agccgaccac ggtcagcaaa gcggcgatcg aggtcatcga aaactcgatc tgcaacgccg
     1981 ccatgatgcc gatggtcagc accagatcgt gcaggatggc gatgaccgcg cccgcgccga
     2041 attgcaggcc ggacgggaag cgaaaccaga tatagaccag catcagcagc agggccaggc
     2101 ccagcgtcat caggccggag cgcagcagtt cgcccgacac cttgggcccg acgacctcgg
     2161 tgcgcttgta cgacacgccg gggaaggcgg cggacaggcg ggtcttgacc gcctcgacgc
     2221 cttcggtctg ctcgccttcg tgcagcttga acttgatgat cacgtcgttg ctgcccgacg
     2281 actggaccgt cacgtccgag gcgccgaagc tgggcatgcg cgcgcgcaat tcggccagcg
     2341 gcgccggtcc cggcgtctgc acctcgatga cgttgccgcc ggcgaagtcg g
//
LOCUS       DASVLL010000647         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_367011,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000647 DASVLL010000000
VERSION     DASVLL010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367011"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VF402_10640"
     CDS             complement(<1..641)
                     /locus_tag="VF402_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7800800.1"
                     /translation="MPSRPEQTPEANLAAPGAGPVVAFERVSLGYGLGPGQPPLSGED
                     DPRLILKNLTFQLPAGSFHFLTGPSGAGKTSLLKLIYLAQQPSGGRISLFGEAIRAHD
                     RAQSARLRRQLGIVFQEFRLLDHLNVFDNAALPLFVHGRNPAGYRDDVTELLSWVGLK
                     HRIHAMPELLSGGEKQRLAIARAVVTRPTLIVADEPTGSIDYAMGLRIMRLFI"
     gene            761..1636
                     /locus_tag="VF402_10645"
     CDS             761..1636
                     /locus_tag="VF402_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEX7800801.1"
                     /translation="MILSCPHCGTRYSLSESQLGPNGRKVRCSACKTTWHADPPPAEP
                     IELAPEPPVKPRVEDLQQVKAERLPSRYRALIEDKKRLRALTVQGLVWGGLAAAFVAV
                     LTLAFALRVDIVRAFPRIAGAYAMVGVKVNATHLEFGAKSADASFKGGRFYVTVKAQV
                     RNVSDKTVPVPPVQVRLMDASLQTFATTLIPSNGLSVPAHQTRTLTFDVADPKNMTSS
                     VDLDFDLEALKAGMRAGKAPVLRTATAQAAEAPPDAADADAESAASPAVTGRPTPPLR
                     TALADADGHDLGAAP"
     gene            1639..2169
                     /locus_tag="VF402_10650"
     CDS             1639..2169
                     /locus_tag="VF402_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7800802.1"
                     /translation="MTTPSVLLPETEVQARVDVLADLLAHYIRDTCVGVCLLTGGIWF
                     AADLTRALNRRGRDLAFDALWLSSYGDVREAGTLLIRAPLQRPVEGRQVLIIDDVLDT
                     GASLKIARDIAYEAGASEVLTCVFARKPDPLKAGARREIEADFCAWEAPARYLVGYGL
                     DDAGRFRALPYIGVLD"
     gene            2227..2523
                     /locus_tag="VF402_10655"
     CDS             2227..2523
                     /locus_tag="VF402_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEX7800803.1"
                     /translation="MPTVPVQTRIDASVKERAGIVLERMGLTVSDAVRILLTRIATEG
                     ALPAGLTVDPEAYDAWFRAKVQEALDDPRPPLSHEEVEAHFAARRAAALRKPER"
     gene            2526..2807
                     /locus_tag="VF402_10660"
     CDS             2526..2807
                     /locus_tag="VF402_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7800804.1"
                     /translation="MRLTWSFQAQNDRNRIFDYIEADNPRAAVAVDNAIREQIEHLTQ
                     YPQSGRAGRVSGTRERVVTGLPYLVVYRLLDDGIRILRIVHTSQLWPTP"
BASE COUNT          479 a          966 c          938 g          474 t
ORIGIN      
        1 tcgatgaaca ggcgcatgat gcgcaggccc atggcgtagt cgatgctgcc ggtcggctcg
       61 tcggcgacga tcagggtcgg gcgcgtcacc acggcgcgcg cgatggccag ccgctgcttc
      121 tcgccgccgg acagaagctc cggcatggcg tggatgcggt gtttcagccc gacccagctc
      181 aacagctccg tcacatcgtc gcgataaccc gccgggttgc ggccatgcac gaacaggggc
      241 agggccgcgt tgtcgaaaac gttaagatga tcgagcaggc ggaattcctg aaacacgatg
      301 ccgagctggc ggcgcaggcg ggcgctttgc gcgcggtcat gggcgcggat cgcctcgccg
      361 aacagcgaga tgcggccgcc cgacggctgt tgcgccagat agatcagctt caaaagcgag
      421 gtcttgcccg cgccggacgg gccggtcagg aaatggaacg aacccgcagg cagttgaaac
      481 gtcaggtttt tcaggatcag gcgcggatcg tcctcgccgc tcagcggcgg ctggcccggc
      541 cccagcccat agccgagcga gacgcgctcg aacgccacga ccggcccggc gccgggcgcg
      601 gcgaggttcg cttcgggagt ctgttccggg cgggacggca tggcgcgatc gaaggcctca
      661 gacttgatca gttgttaaga cttttcggga cataaggtta aaaaagccgt atttggttaa
      721 tacgggctta tgcagtgatt cggctgttgt ctgagcgatc atgatcctga gttgccccca
      781 ttgcggtacc cgttacagcc tttctgagtc ccaattgggg ccgaacggcc gcaaggtgcg
      841 ctgctccgcc tgcaagacga cctggcacgc cgatccgcca cccgccgaac cgatcgaact
      901 ggcgccggaa ccgccggtga agccgcgcgt cgaggatttg cagcaggtca aggccgagcg
      961 cctgccgtcg cgctatcgcg cgctgatcga ggacaagaag cggctgcgcg ccctgaccgt
     1021 gcagggactg gtctggggcg ggctggccgc cgccttcgtc gccgtcctga ccctggcctt
     1081 cgcgctgcgc gtcgacatcg tgcgcgcctt cccgcgcatc gccggggcct atgcgatggt
     1141 cggggtcaag gtcaacgcca cccatctgga gttcggcgcc aagagcgccg acgcctcgtt
     1201 caaaggcggg cgcttctacg tcaccgtcaa ggcgcaggtc aggaacgtca gcgacaagac
     1261 cgtccccgtg ccgccggtgc aggtccggct gatggacgcc agcctgcaaa ccttcgccac
     1321 cacgctgatc ccgtcgaacg gcctgagcgt cccggcgcat cagacgcgca ccctgacctt
     1381 cgacgtcgcc gatccgaaga acatgacctc gtccgtcgat ctggatttcg atctggaagc
     1441 cctgaaagcc gggatgaggg ccggtaaggc gccggtcctg cgcacggcga cggcgcaggc
     1501 cgcggaagcg ccgccggacg ccgccgacgc cgacgccgaa tccgccgcct cccccgccgt
     1561 cacgggccgt ccgacgccgc cgctgcgcac cgcgctggcc gacgccgacg gccacgatct
     1621 cggagccgcg ccctgaacat gaccacgccg tccgttcttt tgcccgaaac cgaggtgcag
     1681 gcgcgcgtcg acgtgctggc cgacctgctg gcgcattata tccgcgatac gtgcgtcggg
     1741 gtctgtctgc tgaccggcgg catctggttc gccgccgacc tgacgcgggc gctgaaccgg
     1801 cgcgggcgcg acctcgcctt cgacgccctg tggctgtcct cctacggcga cgtgcgcgaa
     1861 gccggaaccc tgctgatccg cgcgccgttg cagcgtccgg tcgagggccg ccaggtgctg
     1921 atcatcgacg acgtgctgga cactggcgcc tcgttgaaga tcgcgcgcga tatcgcttac
     1981 gaagcgggcg catcggaagt gctgacctgc gtgttcgcgc gcaagcccga cccgctgaaa
     2041 gccggcgcgc gccgcgagat cgaggcggac ttctgcgcct gggaagcccc cgcgcgctat
     2101 ctggtcggct acggactgga cgacgccggc cgcttccgcg ccctgcccta catcggggtt
     2161 ctggattaga ctggtcattg tcatgcaatt gcattacaat gacggcgttg tcttcgagtg
     2221 accgccatgc cgaccgtccc cgttcaaacc cgcatagacg cctctgtgaa agaacgcgcc
     2281 ggtatcgttc tggaacgcat gggcctcacg gtttcggacg cggtgcgcat tctgctgacc
     2341 cgcatcgcca cggagggcgc cttgcccgca ggactgacgg tcgatcccga agcctatgac
     2401 gcatggtttc gcgccaaggt tcaggaagcc ctcgacgatc cgcgtccgcc gctttcgcat
     2461 gaggaggtcg aagcgcactt cgccgcgcgc cgcgccgccg ccttgcgaaa accggagcgg
     2521 taagcgtgcg gctcacatgg tcgtttcagg cgcaaaatga ccgcaaccgg attttcgact
     2581 atatcgaagc cgacaacccg cgcgccgccg tcgccgtcga caacgccatc cgggaacaga
     2641 ttgagcacct gacgcagtat ccgcaaagcg ggcgcgccgg gcgcgtcagc ggcacgcgcg
     2701 aacgggtcgt caccggcttg ccctatcttg tcgtctatcg gctgctggat gacggcatac
     2761 ggattctgcg gatagtgcac acgtctcagc tttggccgac cccttagttc cggctggcca
     2821 gagtcaaagt ccgcgctatg tccggcgtga aggccga
//
LOCUS       DASVLL010000648         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_205777,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000648 DASVLL010000000
VERSION     DASVLL010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205777"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VF402_10665"
     CDS             <1..1593
                     /locus_tag="VF402_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX7800805.1"
                     /translation="IQTRNGWGPVTRVDVASQKVGEVWTPPANLLSGYQPGSVRVEVS
                     YSPFRNIDPGPLAAALNVYPYGCTEQVASSATPWLFVSESLVGKAKAAPADAALQQAV
                     TKILDRQSADGAFGLWRAGDGEADGFIGAYATDFLLEARARGVFVPQDAIDKALNAMR
                     DLSRPDGYISLNYRLSAPDSWRWLGVSGEQITKQMRSRASAYALYVLAKAHSGDLARL
                     RWYQDAQFKDEQSPLARAQVAAGLAMMGDRARARLAFTQAIQTLGYADPNDWYQSPLR
                     DLAGVIALAYESGFDDIARSLVPRLENAMKSPADMNTQEDAAVLRAAAYMLKASGAPK
                     IDAQGVSALPGPGLHYGVGALNAAHLRNSGSGPIWRTVTVIGTPVQSPAADSHGLSLT
                     KTYYGLDGSRIDPSRLTQGQRVIVVVTGRSDRAEQRPIVVDDPLPAGFEIDSVLSAED
                     AQNGPFGFIGKISDLKVSEARDDRYVAALDVSSDHGFTLAYIARAVTPGDYYLPGAEA
                     RDFYRADTFARTGGGRTVIAPR"
BASE COUNT          278 a          615 c          581 g          266 t
ORIGIN      
        1 atccagacgc gcaacggctg gggaccggtc acgcgcgtcg atgtcgcttc gcaaaaggtc
       61 ggcgaggtct ggaccccgcc cgccaatctg ctcagcggct atcagcccgg ctcggtcagg
      121 gtcgaggtca gctattcgcc cttccgcaat atcgaccccg gcccgttggc cgccgcgctc
      181 aacgtctatc cctacggctg caccgagcag gtggcgtcgt cggcgacgcc gtggctgttc
      241 gtcagcgaat ccctggtcgg caaggcgaag gccgcgcccg ccgatgccgc gctgcaacag
      301 gcggtgacca agatcctcga ccgccagagc gccgacgggg ctttcggcct gtggcgcgcg
      361 ggcgacggcg aggcggacgg cttcatcggc gcctatgcga ccgacttcct gctggaagcg
      421 cgggcgcgtg gggtgttcgt gccgcaggac gccatcgaca aggcgctgaa cgccatgcgc
      481 gacctgtcgc ggcccgacgg ctacatcagc ctgaactatc gcctcagtgc gccggactcc
      541 tggcgctggc tcggcgtctc tggcgagcag atcaccaaac agatgcggtc gcgcgcctcg
      601 gcctatgcgc tctacgtgct ggccaaggcc cattccggcg atctggcgcg cctgcgctgg
      661 tatcaggacg cgcagttcaa ggacgaacaa tcgccgctgg cgcgcgcgca ggtggcggcg
      721 ggtctggcca tgatgggcga cagggcgcgc gcgcggctgg ccttcacgca ggcgatccag
      781 acgctcggtt acgccgatcc gaacgactgg tatcaatcgc cgctgcgcga tctggccgga
      841 gtgatcgcgc tggcctacga atcgggcttc gacgacatcg cccgttcgct ggtcccgcga
      901 ctggaaaacg ccatgaagtc gcccgccgac atgaacacgc aggaggacgc cgcggtcttg
      961 cgcgccgccg cctatatgct caaggcgtcg ggcgcgccga agatcgacgc gcagggcgtc
     1021 agcgctctgc cgggaccggg cctgcattac ggcgtcggcg ccctgaacgc ggcgcatttg
     1081 cgcaacagcg gctcaggccc gatctggcgc acggtgacgg tgatcggaac cccggtgcaa
     1141 agccccgccg ccgacagcca cggcctgagc ctgaccaaga catattacgg tcttgacggt
     1201 tcgcgcatag acccgtcgcg tctgacgcaa ggccagcgcg tgatcgtggt cgtcaccggc
     1261 cgctccgacc gcgccgagca acggcccatc gtggtcgacg atcctctgcc cgccgggttc
     1321 gagatcgatt cggtgctgtc cgccgaggac gcgcagaacg ggccgttcgg cttcatcggc
     1381 aagatcagcg acctgaaggt ctccgaggcg cgcgacgacc gctatgtcgc cgccctcgac
     1441 gtctcgtccg atcacggctt caccctcgcc tatatcgcgc gcgccgtgac gccgggcgac
     1501 tactacctgc ccggcgccga ggccagggac ttctaccgtg ccgatacgtt cgcccgcacc
     1561 ggcggcgggc gcacggtgat cgcgccgaga tgaaaaaacc tgctgagtcc ccctctcccc
     1621 tccacgaggg gagaggacga ggcgcaaggc gaagtcgccg atcctcgggt gaggggcagc
     1681 ccgacggttg cgctcgatcg gcgaacagcc cctcacccga tcgccttcac tacgttcggc
//
LOCUS       DASVLL010000649         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_156090,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000649 DASVLL010000000
VERSION     DASVLL010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156090"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF402_10670"
     CDS             <1..279
                     /locus_tag="VF402_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEX7800806.1"
                     /translation="IVGTIAELRAGADAIARSVAAQQTATSRIADTVESLAAGSRSVG
                     RDIDSVRDAANETGQVASVVFEEAKSVADAAQRLREELARFLESARAA"
     gene            complement(303..376)
                     /locus_tag="VF402_10675"
     tRNA            complement(303..376)
                     /locus_tag="VF402_10675"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Cys,seq:gca)
     gene            581..1210
                     /locus_tag="VF402_10680"
     CDS             581..1210
                     /locus_tag="VF402_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX7800807.1"
                     /translation="MDFHASRQNMVESQVRVNDVTDQALVAAMRTVRRERLCAPSESF
                     RAYAEVEPTIAPERVLMAPRDVSKLLQTLEPVAGESALALAAPYAAAVLAEAGLKVTA
                     QEADPRAMAVIEPYLKELGVATRTQDLKTPSGADYDVIVVEGAVEEVPENWLKALKVG
                     GRLAVVLKDGPVGRARIYLRLASGYSERDAFDSSPSTLPGFRRAPSFQF"
     gene            1450..2853
                     /locus_tag="VF402_10685"
     CDS             1450..2853
                     /locus_tag="VF402_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX7800808.1"
                     /translation="MMKSKLGGKPFAASLAVLLMAVAASGTAHAESLGDAIAMAYAQN
                     PTLVRARAQQRAADEGYVQARSSLGPSLSAGASTDYADHVDPQGNRSYTGLSLSAAQT
                     LFASGGLSSTLAASKATVLSGQQSLRSTEQGVLLDVIDIYTAVQRDQQALKISQDNYD
                     ILKQQLDQTQAQFDAGQLTRTDVAQSQARLAASAASLASAQAQLDTDRAAYVAIVGQA
                     PTALDPVPTLPGLPTDLNAALDAAQKNNPDMQAASYAEQSAADRVRAARSVFGPTIGL
                     SAQSGYSAPTNHLGTLSDSESTSITARLSIPLFSAGLNGSRVREAKENLAAQTATVEL
                     TRRSVVSTVSQAWSQMLAARSAVTSNEEQVKAAQIAADGVKTEQQVGLRTNIEVLNAE
                     QDLKNAQLALIEAQRNAYVAGGQLLSVMGALDARAFAPDVQVYDPQANFDKVKNKGWS
                     PLEPVARLLDGLADGGR"
     gene            3047..3580
                     /locus_tag="VF402_10690"
     CDS             3047..3580
                     /locus_tag="VF402_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX7800809.1"
                     /translation="MAEQSAHEPTMEEILASIRRIISEDDAPADEAAEPEAAAAPEPE
                     PEPVADTAEDEEPEEDVLELTDAYDAPASVSIGDIEAYQPEAEPDFEAAEPAPAAPRA
                     AGGGLVSEHTETAALSSFSALTSSLLVPSTDRTLEDVVRDLLRPLLQAWLDQNLPGIV
                     EEQVRAEVERIGRQSRR"
     gene            3679..>4601
                     /locus_tag="VF402_10695"
     CDS             3679..>4601
                     /locus_tag="VF402_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7800810.1"
                     /translation="MLDKTFDPQSVEPRLYEMWEKSGAFAPSDAGEAFSIVIPPPNVT
                     GSLHIGHALNNTLQDVLIRFERMRGKAALWLPGTDHAGIATQMVVERQLAAAGNEGRR
                     DMGRDAFVDKVWAWKAESGGTIVRQLRRLGASCDWSRERFTLDDGLSAAVRKVFVTLH
                     KQGLMYRDKRLVNWDPQFQTAISDLEVEQKEVDGHYWHFAYTLADDLSYEHPVKDEDG
                     RDAVERRDYIVVATTRPETLLGDTAVAVHPDDPRYASLIGKSVILPITGRRIPIVADE
                     YADPTKGSGAVKITPAHDFNDFQVGKRHNLP"
BASE COUNT          808 a         1487 c         1564 g          742 t
ORIGIN      
        1 atcgtcggca ccatcgccga actgcgcgcg ggcgccgacg ccatcgcccg ctcggtggcg
       61 gcgcagcaga cggcgaccag ccgcatcgcc gacacggtcg aatcgctggc cgccggttcg
      121 cgcagcgtcg gacgggacat cgattcggtg cgggacgcgg cgaacgagac gggacaggtg
      181 gccagcgtcg tgttcgagga ggcgaaatcc gtcgccgacg ccgctcagcg gctgcgcgag
      241 gaactggcgc gcttcctgga atcggcgcgc gcggcgtaaa gccttgacgg cgtggggaag
      301 cgtggaggcc tgagccggag tcgaaccggc ctacacggat ttgcaatccg ctgcgtaacc
      361 gctccgccat caggcctcac aggagcgaag cggtgagcta tcagatcgtt cacgcgcctg
      421 caacgatgat taggacattt ttcccaagat atttttaaaa tcgccgcgcg atgcggcttt
      481 cgcggcgcaa accgtgcaga aaaagccgtt gaagcgtccg gcgacttgcg cttataagcg
      541 gcggatcaaa gttgcgtctt gtcccggaag gaagccgtcg atggattttc acgccagccg
      601 tcagaacatg gtcgaatccc aggtgcgggt gaatgacgtc acggatcagg ctctggtcgc
      661 cgccatgcgc acggtgcggc gcgagcgcct gtgcgcgccg tcggaatcct tccgcgccta
      721 tgccgaggtc gagccgacaa tcgcgccgga gcgcgtgctg atggcgccgc gcgacgtctc
      781 caagctgttg cagacgctgg agccggtcgc gggcgaaagc gcgctggcgc tggccgcgcc
      841 ctacgccgcc gccgtgctgg ccgaggcggg gctgaaggtc acggcgcagg aagccgatcc
      901 gcgcgccatg gcggtcatcg agccgtatct gaaggagctg ggcgtcgcga cccggacgca
      961 ggatctgaaa acgccgtcgg gcgcggatta cgacgtgatc gtcgtcgagg gcgcggtcga
     1021 ggaagtgccc gaaaactggc tcaaggcgct caaggtcggc ggccgtctcg ccgtggtgct
     1081 gaaagacggt ccggtcggcc gcgcgcgcat ctatctgcgt ctggcgtcgg gctattccga
     1141 gcgcgacgcg ttcgattcgt cgccctcgac cctgccgggc ttccggcgcg cgccgtcttt
     1201 tcagttttaa gaaacggtca gctttaagaa cgctggcaac tgctgtcatc tttggccgcg
     1261 ccgttcgtgt aagggacggc gcggcctttt tgcgccgcat gaaaatcgca aaggtgaaaa
     1321 atccttaact cgccaagttt atgaacttct ggcatctggg ggatttggag ctaatctgtc
     1381 gccggcgcga aagcccgccg tattccagcg gggcgcggcc ggtcatcggc atattcgggg
     1441 tgctaattta tgatgaagtc gaaattgggc ggtaagccgt tcgccgcgtc gctggcggtg
     1501 ttgctcatgg cggtcgcggc gtcgggaacg gcgcacgccg aatcgctggg cgacgccatc
     1561 gccatggcct atgcgcagaa ccccaccctc gtgcgcgcgc gtgcgcagca gcgcgccgcc
     1621 gacgaaggtt atgtgcaggc gcgctcgtcg ctggggccgt cgctgagcgc gggcgcttcg
     1681 accgattacg ccgatcacgt cgatcctcaa ggcaaccgtt cctacaccgg cctgtcgctg
     1741 tcggcggcgc agaccctgtt cgcctcgggc gggctgagtt cgacgctggc ggcgtccaag
     1801 gcgacggtgc tgtcgggcca gcagagcctg cgctcgaccg agcagggcgt gctgctggac
     1861 gtgatcgaca tctacaccgc cgtgcagcgc gatcagcagg cgctgaaaat ctcgcaggac
     1921 aattacgaca tcctcaagca gcaactggac cagacgcagg cgcagttcga cgccgggcaa
     1981 ctgacccgca ccgacgtggc gcagtcgcag gcgcggctgg cggcgtcggc ggcgtcgctg
     2041 gcctcggcgc aggcgcaact cgacaccgac cgcgccgcct atgtcgccat cgtcggccag
     2101 gcgccgacgg cgctcgatcc cgtgccgacg ctgcccggcc tgcccacgga cctcaacgcc
     2161 gcgctcgacg ccgcgcagaa gaacaatccc gacatgcagg ccgcgagcta tgccgaacaa
     2221 tcggcggcgg atcgcgtccg cgccgcgcgt tcggtgttcg gccccaccat cggcctgtcg
     2281 gcgcagtcgg gctattcggc gccgaccaac cacctcggca cgctgagcga cagcgaaagc
     2341 accagcatca ccgcgcgcct gtcgatcccg ctgttcagcg cgggcctgaa cggttcgcgc
     2401 gtgcgcgagg ccaaggaaaa cctggccgcc cagaccgcga cggtcgaact gacgcgtcgc
     2461 agcgtcgtct ccaccgtgtc gcaggcgtgg tcgcagatgc tggccgcgcg ctcggcggtg
     2521 acgtcgaacg aggagcaggt caaggccgcg cagatcgccg ccgacggcgt caagaccgag
     2581 cagcaggtcg gcctgcgcac caatatcgag gtgctgaacg ccgagcagga cttaaagaac
     2641 gcgcaactgg cgctgatcga ggcgcagcgc aacgcatatg tcgcgggcgg gcagcttctg
     2701 tcggtcatgg gcgcgctcga cgcccgcgcc ttcgcgccgg acgttcaggt ttacgatccg
     2761 caggccaatt tcgacaaggt gaagaacaag ggctggtcgc cgctggagcc ggtggcgcgc
     2821 ctgctggacg ggctggccga cgggggcaga taagcgcctg gccgggcgtt ttttaaggga
     2881 cgcataaggg gcgcgcgcgg cgcccctttt tgttttgcgc cgaaattagc cgattttgac
     2941 gaaaaacggg tgggaaaccg cccttgacgg ttgccaaaat cgccgaacgc gtgtcaccta
     3001 gcgttggttt cagttccgca aaccgattcg cagcagttaa aagacgatgg cagaacaatc
     3061 cgcacacgaa ccgaccatgg aggagattct cgcctccatt cgccgcatca tctccgagga
     3121 cgatgcgccg gccgacgaag ccgccgagcc ggaagctgcc gccgcgcccg aaccggagcc
     3181 ggagcccgtc gccgacacgg ccgaggacga ggagcccgag gaggacgttc tggagctgac
     3241 ggacgcctat gacgcgcccg cgtcggtctc catcggcgac atcgaggctt accagcccga
     3301 agccgaaccc gatttcgagg ccgccgaacc cgcgcccgcc gcgccgcgcg ccgcaggcgg
     3361 cggtctggtc agcgagcata cggaaacggc ggccctgtcg tcgttcagcg ccctgaccag
     3421 ttcgctgctg gtgcccagca cggaccgcac gctggaggac gtggtgcgcg acctgctgcg
     3481 gccgctgttg caggcgtggc tcgatcagaa cctgcccggc atcgtcgagg aacaggtgcg
     3541 cgccgaggtc gaacgcatcg ggcgccaatc gcgccgctaa gttcgcgcta aggcatggat
     3601 tttcagggtt tgacctgata agagggcggc gtcggcatcg aacgccgccc ttctcgtttc
     3661 tctttttccc tctgagccat gttagacaag acctttgatc cgcaatccgt tgaaccgcgc
     3721 ctttatgaga tgtgggaaaa gtcgggcgcc tttgcgccgt ccgatgcggg cgaagccttc
     3781 tcgatcgtga ttccgccgcc caatgtcacg ggcagcctgc acatcggcca tgcgctcaac
     3841 aacacgcttc aggacgtgct gatccgcttc gagcgcatgc gcggcaaggc ggccttgtgg
     3901 ctgcccggta cggaccacgc gggcatcgcg acgcagatgg tggtcgagcg gcaactggcg
     3961 gcggcaggca atgaaggccg ccgcgatatg ggccgcgacg cctttgtcga caaggtctgg
     4021 gcgtggaagg cggaatcggg cgggaccatc gtgcggcagt tgcgccgtct cggcgcctcg
     4081 tgcgactggt cgcgtgagcg gttcacgctg gacgacgggc tgtcggcggc ggtgcgcaag
     4141 gtgttcgtga ccctgcacaa gcagggcctg atgtaccgcg acaagcggct ggtcaactgg
     4201 gatccgcagt tccagacggc gatttcggac cttgaggtcg agcagaagga ggtggacggc
     4261 cattactggc atttcgccta cacgttggcc gacgacctgt cctacgaaca tccggtcaag
     4321 gacgaggacg gccgtgacgc ggtcgagcgc cgcgactata tcgtggtggc gaccacgcgc
     4381 ccggaaaccc tgctgggcga cacggcggtg gccgtgcatc ccgacgatcc gcgctatgcg
     4441 agcctgatcg gcaagtccgt catcctgccg atcacgggcc gccgcattcc gatcgtggcc
     4501 gacgaatacg ccgatccgac gaaaggctcc ggcgcggtca agatcacgcc cgcgcacgat
     4561 ttcaacgact ttcaggtcgg caagcggcac aacctgccgc a
//
LOCUS       DASVLL010000650         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_260411,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000650 DASVLL010000000
VERSION     DASVLL010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260411"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VF402_10700"
     CDS             complement(<1..502)
                     /locus_tag="VF402_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7800811.1"
                     /translation="MDKPSAVNAGFDAVLNALKAAGEETRLRLLRLLAREELSVMELV
                     SILSQSQPRVSRHLKLMNEAGLIERFPDGAWVFYRLSGDPAMRPLLEAILAGLGAAHA
                     DDLRQLETVRLARRASAEGYFETIAPTWDEIRSHYVSESDVEAAILRVLGDAPYRRLL
                     DLGTGSG"
     gene            636..1001
                     /locus_tag="VF402_10705"
     CDS             636..1001
                     /locus_tag="VF402_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX7800812.1"
                     /translation="MARDGVTLATALGRGLRRKCPCCGQASAFRGYLKVIDACPVCQT
                     PLSLYPTDDGPAYITMLLIGHVVVGPTFLFPAVWTWPLQIVAPSLVAAIGALTLVALP
                     FVKGGFLSLLWYLGMKQAR"
     gene            1060..1242
                     /locus_tag="VF402_10710"
     CDS             1060..1242
                     /locus_tag="VF402_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HEX7800813.1"
                     /translation="MAHRFEIRKNKAGEFVAYFVYNSETIFWTEGYSSKASAKNAIES
                     IKKNGPEAETVDKTVD"
     gene            complement(1252..1512)
                     /locus_tag="VF402_10715"
     CDS             complement(1252..1512)
                     /locus_tag="VF402_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800814.1"
                     /translation="MANAFPNAASRLYALAVAKDTANLVDADAALALARASTRTLMAL
                     SPQAAQLMRSFAQEEIDRLSMECTEEAQGSIALIRDALSLEA"
BASE COUNT          398 a          671 c          610 g          374 t
ORIGIN      
        1 gcccggagcc ggtgcccaga tcgagcaggc ggcggtacgg cgcgtcgccc agcacgcgca
       61 ggatcgccgc ctcgacgtcg ctttcggaca cgtaatggct gcgaatctcg tcccatgtcg
      121 gcgcgatcgt ctcgaaatag ccttccgccg aggcgcgccg cgccagccgc accgtctcca
      181 gttgccgcag gtcgtccgcg tgcgccgcgc ccagaccggc cagaatcgcc tccagcaacg
      241 gacgcatggc cggatcgccc gacagccggt aaaacaccca ggcgccgtcc gggaagcgtt
      301 cgatcagccc cgcctcgttc atcagcttga gatggcgcga cacgcgcggc tggctctggc
      361 tcaggatcga caccagctcc atcaccgaca gctcctcgcg cgccagcagg cgcaaaaggc
      421 gcaggcgcgt ctcctcgccc gccgctttca gggcgttgag gacggcgtcg aaaccggcgt
      481 tgacggcgga gggcttatcc atataatcat ataaagatat ctttatatga tacgcaagcg
      541 cttttttcgt cccctcaccc ctgcgtcaca gggacgcatg gcgcccttat cgcgatcata
      601 acatagtcgg gacggaatac acagaaggag ccttcatggc cagagacgga gtgacgctgg
      661 cgacggcgct ggggcgcggc ctccggcgca aatgcccctg ctgcggccag gcgtccgcgt
      721 ttcgcggcta tctgaaggtg atcgacgcct gccccgtctg ccagacgccg ctcagcctct
      781 atcccaccga cgacggcccg gcctatatca ccatgctgct gatcgggcat gtggtggtcg
      841 ggccgacctt tctgtttccg gcggtgtgga cgtggccgct gcaaatcgtc gcgccgtccc
      901 tggtcgcggc gatcggcgcg ctgacgctcg tggccctgcc cttcgtcaag ggcggtttcc
      961 tcagcctgct ctggtatctg ggcatgaaac aggcgcgcta agacgcttga caggcgcgcg
     1021 gaacaggcgc cagataccag catccgaggg agatgcgaca tggcgcacag gtttgaaatc
     1081 cgcaagaaca aggccggcga attcgtcgcc tacttcgttt acaacagcga gacgatcttc
     1141 tggaccgagg gctacagctc gaaagcctcg gcgaagaacg ccatcgaatc gatcaagaag
     1201 aacgggccgg aggccgagac cgtcgacaag acggtcgatt aaggcgcgcc tctaagcctc
     1261 gaggctgagc gcgtcgcgaa tcagggcgat cgagccttgc gcctcttcgg tgcattccat
     1321 cgacaggcgg tcgatctcct cttgcgcgaa gctgcgcatc agttgcgccg cctgcggcga
     1381 cagggccatc agggtgcgcg tcgaggcgcg cgccagggcc agggccgcat cggcgtcgac
     1441 aaggttcgca gtgtctttgg ccaccgccag cgcataaagg cgcgaggcgg cattgggaaa
     1501 cgcgttcgcc atcgttttct tcttggacat gggtgttttt accagatctc tccacggtcc
     1561 gggcgagcac acacaccaca cacgatgcgc tcaggtgaac cgtacgctta cgcctactca
     1621 accggcacgg aatatattcg cgtttgtgac gaaatccaga cgcccgccgt ttcagggctg
     1681 aaaaacgcgc cggcataatt cggttatgtt ttcagcttct tgcaaaaacg cttcgtacaa
     1741 aatttcacga aagcgtgata ataagtaaaa aatttcatgt tagcgctacc gcgcacgact
     1801 ttattctaag aaaagcataa atttcctaca ggcaagaaca accgcgccgg aaatccggcc
     1861 cgcgttttca ggccgtatcc ccctccctac ccctctcccc tccacgaggg gagaggacga
     1921 cctgcaagcc cgatagggcg cagagggtcg ggtgaggggc tgtccgccgt ttgcgctcag
     1981 ccgtccgcca gcccctcacc cgaggatctg cggcttcgcc ttgctcctcg tcctctcccc
     2041 attgtcatgg gga
//
LOCUS       DASVLL010000651         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_475774,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000651 DASVLL010000000
VERSION     DASVLL010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475774"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /gene="uxuA"
                     /locus_tag="VF402_10720"
     CDS             complement(<1..943)
                     /gene="uxuA"
                     /locus_tag="VF402_10720"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEX7800815.1"
                     /translation="MSVPDHLMIQTMRWFGPKDPVSLADIRQAGCEGVVTALHHIPNG
                     EVWPQAAIMARKRQIEAHGMAWSVVESLPVHEAIKTRGPGYETCIENYKTSLRNLAAC
                     GVTVVTYNFMPLLDWTRSDLDYAMPDGARALRFDAVEIAAFDLFHLARPGAGADYTPA
                     MTARARALYDTMSDADLERLERNIIKGLPGSEETFGLDDFRAALRAYDGIDAARLKQN
                     LVDFLSAVCPVADEAGVRLVIHPDDPPFPIFGLPRVVSTAADLDDLFARVPNASNGLC
                     FCAGSFSIRADNRLTEMVERFGERIHFTHLRNTRREIV"
     gene            complement(948..>2119)
                     /locus_tag="VF402_10725"
     CDS             complement(948..>2119)
                     /locus_tag="VF402_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7800816.1"
                     /translation="TGAAQWTSAQWTSAWASAQMPVDAKYALKDAGDLSLRQIVRVTA
                     AGHEARVRLSNAFGKTPLVVLKAAVAPAQGPGSAALAGDSHALTFGGQPGVTLAPGQE
                     IVSDPVDLPVSAFDDLAVSLYRAAAPEPQTGHVAAHATAFRINGDHVADADPSGAARY
                     ESWFALSDIDVLSPASGAVAVLGDSITDGTASPTDANARWTDDLAHRLRDNGQSVAVV
                     NLGIGGNCLLRQCHGPAGLDRLDRDVFAREHVRWLIVAEGINDLGRATKDGGLTPQGH
                     ADLVQRLEDGYRSVIAQGHAHGLKVIGATLTPDGASPLYHPDAAAEADRQALNAWIRS
                     GGAFDGVIDFDAAVRDPADPARLQPAYDSGDHLHPGPGYAVMAAVIDLNLFSDRK"
BASE COUNT          304 a          682 c          797 g          336 t
ORIGIN      
        1 cgacgatctc gcgccttgtg ttgcgcaggt gggtgaagtg gatgcgttcg ccgaagcgct
       61 cgaccatctc ggtcaggcgg ttgtcggcgc ggatgctgaa cgatccggcg cagaagcaca
      121 ggccgttcga ggcgttgggg acgcgcgcga acagatcgtc gagatcggcg gcggtcgaga
      181 cgacgcgcgg caggccgaag atcgggaagg gcggatcgtc gggatggatg accagcctga
      241 cgccggcctc gtccgcgacg gggcagaccg cgctcaggaa atcgaccaga ttctgtttca
      301 ggcgcgcggc gtcgatcccg tcataggcgc gcagcgcggc gcggaagtcg tcgaggccga
      361 acgtctcctc actgcccggc aggcccttga tgatgttgcg ctccagccgc tccagatcgg
      421 cgtccgacat cgtatcgtag agcgcgcgcg ccctggcggt catggcgggc gtatagtcgg
      481 ccccggcgcc gggccgcgcg aggtggaaca ggtcgaaggc ggcgatctcg accgcgtcga
      541 agcgcagcgc gcgcgcgccg tcgggcatgg cgtagtcgag gtcggagcgc gtccagtcga
      601 gcagcggcat gaagttgtag gtcacgaccg tgacgccgca ggcggcgagg ttgcgcaggc
      661 tggtcttgta gttctcgata caggtttcgt aacccggccc gcgcgtcttg atcgcctcgt
      721 gcacgggcag ggattcgacc acgctccacg ccatgccgtg cgcctcgatc tggcgtttgc
      781 gcgccatgat cgccgcctgc ggccacactt cgccgttggg gatgtgatgc agggcggtga
      841 ccacgccttc gcagcccgcc tgacggatgt cggcgaggct gacggggtct ttcgggccga
      901 accagcgcat ggtctggatc atcagatggt cggggacgga catgggatta cttccggtct
      961 gagaacaggt tgagatcgat gacggcggcc atgacggcgt agccggggcc gggatgcaga
     1021 tggtcgccgg aatcgtaagc cggctgcaat ctggcgggat cggcggggtc gcgcacggcg
     1081 gcgtcgaagt cgatcacgcc gtcgaaggcg ccgccgctgc gaatccaggc gttgagcgcc
     1141 tgccggtcgg cttcggcggc ggcgtccggg tggtagagcg ggctggcgcc gtcgggcgtg
     1201 agcgtcgcgc cgatcacctt gaggccgtgg gcgtggccct gcgcgatcac gctgcgatag
     1261 ccgtcctcca gcctttgcac cagatcggcg tggccctgcg gcgtcaggcc gccgtctttc
     1321 gtggcgcggc ccagatcgtt gattccttcg gccacgatca gccagcgcac atgctcgcgc
     1381 gcgaacacgt cgcggtcgag gcggtccagc cccgccgggc cgtggcactg acgcagcagg
     1441 cagttgccgc cgatgccgag attgacgacc gcgaccgatt ggccgttgtc gcgcagccga
     1501 tgcgccagat cgtcggtcca gcgggcgttg gcgtcggtcg ggctggccgt gccgtcggtg
     1561 atcgaatcgc cgagaaccgc gacggcgcca gaggcggggc tcagcacgtc gatgtccgac
     1621 agcgcgaacc acgattcata gcgcgccgcg ccgctcggat cggcgtccgc gacgtgatcg
     1681 ccgttgatgc ggaaggcggt ggcgtgggcg gcgacgtggc cggtctgcgg ctccggcgcc
     1741 gccgcgcggt agaggctgac ggccagatcg tcgaacgcgc tgaccggcag gtcgaccggg
     1801 tcggaaacga tctcctgacc gggcgccagg gtgacgccgg gctggccgcc gaacgtcagg
     1861 gcatggctgt cgcccgccag cgcggcgctt cccggccctt gcgcgggcgc gacggcggcc
     1921 ttgaggacga ccagcggcgt cttgccgaag gcgttggaca gacgcacgcg cgcctcatgg
     1981 cccgccgccg tcacgcgcac gatctggcgc aggctcaggt cgcccgcgtc cttcaatgcg
     2041 tatttggcgt cgaccggcat ctgggcgctg gcccatgctg acgtccattg ggctgaagtc
     2101 cattgggcgg cgcccgtca
//
LOCUS       DASVLL010000652         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_420937,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000652 DASVLL010000000
VERSION     DASVLL010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420937"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF402_10730"
     CDS             complement(<1..311)
                     /locus_tag="VF402_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800817.1"
                     /translation="MTLLLLAGLLPLAWSGLVLVLTRDGSGRDRRQKAVLGVMLAPVI
                     LGGVSAALSRFAPLPVVAPLPALPPLPVEDGARAVAAAGAGGWGTVVMLVWMVWATVA
                     L"
     gene            complement(308..688)
                     /locus_tag="VF402_10735"
     CDS             complement(308..688)
                     /locus_tag="VF402_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX7800818.1"
                     /translation="MNKPSDYELRLLRALWRAGRMSAREVHEASHEATGWSFSATRKT
                     LDRMLEKGLIRLELVHGLKTYVAAQTRLETLAGLMTAFARDVLDMDAPLPVAAFAQSK
                     LIAPEEVDELERLLARLSAPEGEA"
     gene            complement(807..989)
                     /gene="rpmF"
                     /locus_tag="VF402_10740"
     CDS             complement(807..989)
                     /gene="rpmF"
                     /locus_tag="VF402_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7800819.1"
                     /translation="MAVPKRKVSPSRRNMRRAHDALGANAYTEDKDTGELRRPHHVDL
                     KTGMYKGRQVITPKKD"
     gene            complement(1138..1521)
                     /locus_tag="VF402_10745"
     CDS             complement(1138..1521)
                     /locus_tag="VF402_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7800820.1"
                     /translation="MRQLVSSGSPFEGEIGFSRAVRSGARIEVSGTAPIRDGKTAGIG
                     DAYEQTKAALEIIVKAVEDAGGKAGHIIRTRIFLTDISLWKDAARAHGEVFGQIRPAS
                     TFAEVKGLINPEWLVEIEASAQVED"
     gene            complement(1722..>3134)
                     /locus_tag="VF402_10750"
     CDS             complement(1722..>3134)
                     /locus_tag="VF402_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEX7800821.1"
                     /translation="SDIYAFRVVTNSVEECYRALGVLHRAWPCVQERFKDFISTPKSN
                     NYKSLHTTVIGPKGMRIELQIRTEEMDRVAEEGVAAHWGYKNQTYGFDEEAATANGGR
                     NPINSLRNIVAIVENGGDSEDWVEHAKMEMFLDQVFVFSPKGRLISLPRGAMPLDFAF
                     AVHTEVGETAIGCLINGEHKPLRTVLQNGDQVEILTGLEPRVNQDWFSLTVTGRARAA
                     IRRHIRQSEREDFIRVGKTAVERAAAQVEKSLTDISWRPAFDKFNVTSEDELFELCGR
                     GKIPVARVVEALFPGLKLPMALSLDTLTRIKDGVGGLAFVRGTALREGSSVFFSRCCR
                     PVPGDRIVGIIEPDGVHVHSIECETLEALDEEKDRWIDLHWTLNAEKNTLSIARLRVN
                     MQNKPGVLGQACTLIGEARGNIVNIAISARPLDYLDIDFDIEVLDARHIINIAAALRT
                     SPLIESVERVRGDEPADVLA"
BASE COUNT          533 a         1131 c          959 g          511 t
ORIGIN      
        1 cacagcgcga ccgtcgccca gaccatccag accagcatga caaccgtccc ccagccgccg
       61 gcgcccgccg ccgcgacggc gcgcgctcca tcctcgaccg gcaagggcgg cagggccggc
      121 aaaggcgcca cgaccggcag cggcgcgaac cgcgacaagg ccgcgctgac gccccccaga
      181 atcaccggcg ccagcatgac gcccagcacc gccttctgcc gccggtcgcg ccccgaaccg
      241 tcgcgcgtca ggaccaggac cagcccgctc cacgccagcg gcagcaaacc ggccagaagc
      301 agcagcgtca cgcctccccc tccggcgccg acaggcgcgc cagcagccgc tccagttcgt
      361 ccacctcctc cggcgcgatc agcttcgact gcgcgaacgc ggcgacgggc aagggcgcgt
      421 ccatgtccag cacgtcgcgc gcgaacgccg tcatcagccc ggccagcgtc tccagccgcg
      481 tctgcgccgc cacataggtt ttcagcccat gcaccaattc cagccggatc agcccctttt
      541 ccagcatgcg gtccagcgtc ttgcgcgtcg ccgagaacga ccagccggtc gcctcatggc
      601 tggcctcatg cacctcgcgc gcgctcatgc gcccggcgcg ccacagcgcg cgcagcaggc
      661 gcagttcata atcgctgggc ttgttcatcg cggcctccgt ttgcgacgaa ggtcgcacgt
      721 ccgcgacgcc cggtcaagcg cgcgcacgaa aaagcgcggc gaagccgaag cctcgccgcg
      781 ctttcaaacc gtgagcggaa aaaggatcag tcctttttcg gcgtgatcac ctgacggccc
      841 ttgtacatgc cggtcttcag atcgacatgg tgcggacggc gcagctcgcc ggtgtccttg
      901 tcctcggtgt aggcgttggc gcccagggcg tcatgcgcgc gacgcatgtt gcggcgcgag
      961 ggcgatactt ttcgcttggg aacggccatg tccgtgtctc ttctttgtca accgccgaaa
     1021 gcgtccgcct tcaggcacaa aatacgatac gacccacctg cgtgagtcag aaggcgcgcc
     1081 ttatgccgca actctccccc atgatcaagg gctaaatggc ggcggcgcgg gattaattca
     1141 gtcctcgacc tgcgcggacg cctcgatctc gaccagccat tccgggttga tcagcccctt
     1201 gacctcggcg aaggtcgagg ccgggcgaat ctggccgaac acctcgccgt gcgcgcgcgc
     1261 cgcgtccttc cacagcgaaa tgtcggtcag gaagatgcgc gtgcggatga tatgcccggc
     1321 cttgccgccc gcgtcctcga ccgccttgac gatgatctcc agcgccgcct tggtctgctc
     1381 gtaggcgtcg ccgatccccg ccgtcttgcc gtcgcgaatc ggcgccgtgc cggaaacctc
     1441 gatgcgggcg cccgaacgca cggcgcggga aaaaccgatc tcgccctcga aaggcgaacc
     1501 ggacgaaacg agctgacgca taggcgtgcc tccatgacag aacaaggccc cgcctttacc
     1561 tccgccacgc cccgaatctc aacaaaaaaa tccctctcat ccccacaagg tggggaggtg
     1621 gcgttcgcgc agcgaacgac ggaggggtat cttgccgtct ggttacccct ccaccgcttc
     1681 gcggtccccc gccccacctt gtggggagga gagaaaggcg gctaagccag cacgtccgcc
     1741 ggttcatccc cgcgcacgcg ctcgacgctt tcgatcagcg gcgaggtgcg caacgccgcc
     1801 gcgatattga tgatgtggcg cgcgtccagc acctcgatat cgaaatcgat atcgagataa
     1861 tcgagcggcc gggcgctgat ggcgatattg acgatattgc cccgcgcctc gccgatcagc
     1921 gtacacgcct ggcccagcac gcccggcttg ttctgcatgt tgacgcgcag ccgcgcgatc
     1981 gacagcgtgt tcttctccgc gttcagcgtc cagtgcaggt cgatccagcg gtccttttcc
     2041 tcgtccagcg cctccagcgt ctcgcactcg atggaatgga catgcacgcc gtccggctcg
     2101 atgatgccga cgatgcggtc gcccggcacc gggcggcagc agcgggagaa gaacacgctg
     2161 gagccttcgc gcaacgccgt gccgcgcacg aaggccagcc cgcccacgcc gtccttgatg
     2221 cgcgtcagcg tgtccagcga cagcgccatc ggcagcttca ggccggggaa caaagcctcg
     2281 accacgcgcg ccaccggaat cttgccgcgc ccgcacagct cgaacagctc gtcctcgctc
     2341 gtgacgttga acttgtcgaa ggccgggcgc cagctgatgt cggtcagcga cttctcgacc
     2401 tgcgccgccg cgcgctccac cgccgtcttg ccgacgcgga tgaagtcctc gcgctcggac
     2461 tggcggatat ggcggcgaat ggcggcgcgc gcgcgcccgg tcacggtcag cgagaaccag
     2521 tcctgattga cgcgcggctc cagcccggtc aggatctcga cctgatcgcc gttttgcagc
     2581 acggtgcgca gcggcttgtg ctcgccgttg atcaaacagc cgatggccgt ttcgccgacc
     2641 tcggtatgca ccgcgaaggc gaagtcgagc ggcatcgccc cgcgcggcag cgagatcagc
     2701 cgtcccttgg gcgaaaagac gaacacctga tcgaggaaca tctccatctt ggcgtgttcg
     2761 acccagtcct ccgaatcgcc gccgttctcg acgatggcga cgatgttgcg aaggctgttg
     2821 atcgggttgc gcccgccatt ggccgtggcg gcttcctcgt cgaagccgta ggtctggttc
     2881 ttgtagcccc agtgggcggc gacgccctcc tcggccaccc ggtccatctc ctcggtgcgg
     2941 atctgcaact cgatgcgcat ccctttcggc ccgatgaccg tggtgtgcag ggacttgtaa
     3001 ttgttcgact tcggcgtcga gatgaagtcc ttgaagcgct cctgcacgca cggccaggcg
     3061 cggtgcagca cgcccagcgc gcggtaacat tcctcgacgc tgttcgtcac cacgcggaag
     3121 gcgtagatgt ccga
//
LOCUS       DASVLL010000653         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_367420,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000653 DASVLL010000000
VERSION     DASVLL010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367420"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF402_10755"
     CDS             <1..703
                     /locus_tag="VF402_10755"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX7800822.1"
                     /translation="RLRQNLPWVRVDKTYRFDTEQGAATLDDLFQGRSQLLVYHFMFG
                     PDYKAGCASCSAIADGFNGIAVHLANHDVMLWAASRAALPKLQAFKERMGWTFPWAST
                     VGGAFNFDFNVSFTEAQQRDGDGEYNYRSVGGRAMVTTKLPEAVLENAAMTGADALTF
                     MRERPGLSAFVREDDVVYHTYSTYARGLDGLWGMYQWLDRAPKGRNEQGVWWRHHDRY
                     DQAASKADAVVGAQR"
     gene            784..1080
                     /locus_tag="VF402_10760"
     CDS             784..1080
                     /locus_tag="VF402_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800823.1"
                     /translation="MSDVDAGTCDDDANRGDSDRVAPLGFADWLHLAATPTFLAMALS
                     TGVLGGGGQDMPCMAMQDASPLSGMTAMYLLMSGFHLAPWLNLIAGRRRGPIGS"
     gene            1393..>1596
                     /locus_tag="VF402_10765"
     CDS             1393..>1596
                     /locus_tag="VF402_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7800824.1"
                     /translation="MTTKGLSRVTRRRFLKETGLTLAAAGAAPMLSAPFVSRAMAETK
                     SLSIVQWAHFVPEYDTWFDKFAKD"
BASE COUNT          297 a          529 c          505 g          265 t
ORIGIN      
        1 gcggctgcgc cagaatttgc cgtgggtgcg ggtcgacaag acctaccgct tcgacacgga
       61 acaaggggcc gcgacgctgg acgatctgtt tcagggccgc tcgcagctcc tcgtctatca
      121 cttcatgttc ggccccgact acaaggccgg ctgcgcgtcc tgctcggcga tcgccgacgg
      181 cttcaacggc atcgccgtgc atctcgccaa ccacgatgtc atgctgtggg cggcatcgcg
      241 ggcggcgctg ccgaagctgc aggcgttcaa agagcggatg ggctggacgt ttccctgggc
      301 atccaccgtc ggcggcgcgt tcaacttcga cttcaatgtc tccttcaccg aagcccagca
      361 gcgcgacggc gacggcgaat acaattaccg cagcgtcggc ggccgcgcga tggtcacgac
      421 gaagctgccg gaagccgtcc tggagaacgc cgccatgacc ggcgccgacg ccctgacctt
      481 catgcgcgag cggccgggcc tgagcgcctt cgtgcgcgag gacgacgtcg tctaccacac
      541 ctactccacc tatgcccgcg gcctggacgg cctctggggc atgtaccagt ggctcgaccg
      601 cgcgcccaag ggccgcaacg agcaaggcgt ctggtggcgt caccacgacc gctacgacca
      661 ggcggcgagc aaggccgacg cggtcgtcgg cgcccagcgt tgagccgcgc cgcccgcgct
      721 tcatgtgtcc gaattgaaag gttgggcccc tgcgcgggcc caaccgattg cgggagacaa
      781 atcatgagtg atgttgacgc cggcacttgc gatgacgacg cgaaccgcgg cgacagcgac
      841 cgcgtggccc cacttggttt cgccgattgg ttgcacctcg ccgccacacc gacgtttctg
      901 gccatggcgc tttcgaccgg cgtcctcggc ggcggcggac aggacatgcc ctgcatggcc
      961 atgcaggatg cctcgccgct gagcggcatg accgcgatgt acctgctgat gagcggattt
     1021 catctggcgc cgtggctgaa cctgatcgcc ggccgccggc gcggcccaat cgggtcgtga
     1081 tgtcacgcgc gccgcccggc gccaattgat gcaccgccca cgttcgtcaa taagatattg
     1141 gcgcgaaaat aaaatgccgt tcaatgaacg aacggagtca tagcgcgcgg cgccgaatgt
     1201 caccacctct ggccccgtcg aggcggaatt caaattcacg actatgccgc gccgataggt
     1261 caggaatgct gaccggataa atgctggcgc tgcgcggatc acgcgctacg atatcgttct
     1321 agtaaaaaca aaacggtgga ggcggcgctg ccgacgcgcc ccatccgacc gaacccagag
     1381 ggagaacgca gcatgacgac gaaaggtctt tcgcgcgtca cgagacgccg attcttgaag
     1441 gaaactggcc tcacgcttgc cgccgccggt gcggcgccga tgctgtccgc gccattcgtg
     1501 tcgcgggcaa tggccgagac gaaatcattg tccatcgtgc aatgggcgca tttcgtgccc
     1561 gaatacgaca cctggttcga caaattcgcc aaggac
//
LOCUS       DASVLL010000654         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_99607, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000654 DASVLL010000000
VERSION     DASVLL010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99607"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VF402_10770"
     CDS             <1..390
                     /locus_tag="VF402_10770"
                     /inference="COORDINATES: protein motif:HMM:NF022810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEX7800825.1"
                     /translation="LQGALLAFLRAQDSLTAWLGTPPRVWDQPPGEPVCPYVTFGRGR
                     MQAIGGVDAEVAEQTLNLMCVSRFGGAEEARAVAGALRALLDGAALSLSDQVLCSLRV
                     VFVDVFRAADMRTTYALIRLRAVSEPR"
     gene            529..942
                     /locus_tag="VF402_10775"
     CDS             529..942
                     /locus_tag="VF402_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEX7800826.1"
                     /translation="MAVQAGRDMLLKISDGAGTPVFQTVAGLRARTVSLNAKTVDATD
                     SDSGGWRELLAGGGVKSLAVSGSGVFRDAASDALMQAAFFAQDARDWQIVVPGFGVFS
                     GAFLIAALEYAGQHDGEATFAVSLASTGEVSFAAV"
     gene            929..1204
                     /locus_tag="VF402_10780"
     CDS             929..1204
                     /locus_tag="VF402_10780"
                     /inference="COORDINATES: protein motif:HMM:NF023264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTA-gp10 family protein"
                     /protein_id="HEX7800827.1"
                     /translation="MRLSNPARGEVAVVIGGREARLCVTLGALAALEGHFGVSGFAAL
                     GERLKALGPGDLAVVLRALLVDDAPVEAASLAEAMAAVTAAFAAMSA"
     gene            1201..1380
                     /locus_tag="VF402_10785"
     CDS             1201..1380
                     /locus_tag="VF402_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEX7800828.1"
                     /translation="MNWALLLRRALMIGLTPEVFWRLSWREWAMLNGAPDGSPLGRAR
                     LEEMMRLYPDGDGHE"
BASE COUNT          193 a          432 c          623 g          259 t
ORIGIN      
        1 cttcagggcg cgcttctcgc cttcctgcgc gcgcaggaca gtctgaccgc ctggttgggg
       61 acgccgccgc gggtctggga ccagccgccc ggcgaaccgg tctgtcctta cgtgaccttc
      121 gggcgcggac ggatgcaggc gatcggcggc gtcgacgccg aggtcgccga gcagacgctg
      181 aacctgatgt gcgtatcgcg cttcggcggc gcggaggagg cgcgggcggt ggcgggggcc
      241 ttgcgcgcgc tgctggacgg cgcggcgctg agcctgagcg atcaggttct gtgcagtctg
      301 cgcgtggtgt tcgtcgatgt gttccgcgcg gcggatatgc gcacgaccta tgcgctcatc
      361 cgtttgaggg cggtttccga acctaggtaa gaacccctgc cgtcacgccc ttcgggcgcg
      421 gtcctcctcc ttcttttcga tggggagata tgaggcgccg tgcttgaggc gtgcggaaaa
      481 atcgcgctcg ggaagcgcag cgttagcgca aaaaccaagg agatacagat ggctgtgcag
      541 gcgggcaggg atatgctgct caagatttcc gacggcgccg ggacgccggt gtttcagacg
      601 gtggcgggcc tcagggcccg gaccgtgtcg ctgaacgcca agacggtcga tgcgacggat
      661 tcggactcgg gcggatggcg cgagctgctg gcgggcggcg gggtgaagtc gctggccgtg
      721 tcgggttcgg gggtgttccg cgacgcggcg tccgacgcgc tgatgcaggc ggcgttcttc
      781 gcgcaggacg cgcgggactg gcagatcgtc gtgccgggtt tcggcgtgtt ttccggggcg
      841 ttcctgatcg cggcgctgga atatgccggg cagcacgacg gcgaggcgac gttcgccgtc
      901 tcgctggcct cgacgggcga ggtgagcttt gcggctgtct aatccggcgc gcggcgaggt
      961 cgcggtcgtc atcggcgggc gcgaggcgcg tctgtgcgtg acgctgggcg cgctggcggc
     1021 gctggaaggc catttcggcg tgtcgggttt tgcggcgctg ggcgaacggc tgaaggcgct
     1081 ggggccgggc gatctggccg tcgtgctgcg cgccctgctg gtggacgacg cgccggtcga
     1141 ggcggccagc ctcgccgagg cgatggcggc ggtgacggcg gcgttcgcgg cgatgagcgc
     1201 atgaactggg cgcttcttct gcggcgggcc ctgatgatcg ggctgacgcc ggaggttttc
     1261 tggcggctga gctggcgcga atgggcgatg ctgaacggcg cgccggacgg ttcgccgctg
     1321 gggcgggcgc ggctggagga aatgatgcgg ctttatccgg atggtgacgg tcatgaatga
     1381 ggcgttaggc gggctggacg cgcgcgcggc ggagagcgcg gcggcgctga aggcgctgga
     1441 ggcgcccgcg acggaagcgg cgaatacgat cgatcaggcg ttcgcgaaag cgggccagag
     1501 cctggcg
//
LOCUS       DASVLL010000655         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_314905,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000655 DASVLL010000000
VERSION     DASVLL010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314905"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..420
                     /locus_tag="VF402_10790"
     CDS             31..420
                     /locus_tag="VF402_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800829.1"
                     /translation="MSSSNPKHDLEKASTTPGLPLSLGIGPLIEKADVERHPRAIDMR
                     SLMICALCMVLAAVVAPVARLLMLLIGLITNLVFFGRVSGELVSPAGSPLGVWIVVMP
                     VIGSIIVGLMARYGAKAIRGHGIPEAM"
     gene            445..1455
                     /locus_tag="VF402_10795"
     CDS             445..1455
                     /locus_tag="VF402_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX7800830.1"
                     /translation="MPVRMTFLKPLSAGAAIGTGGPFGAEGPIIATGGALGSLLGQLI
                     RVSAHERKVLLAAGAAAGMTAIFATPFSAILLAIELLVFEFRPRSFIPIALAALTAIW
                     LRPWLFGVHPVFAVPLLPAASQTALIGYVLEGLFMVVLAVGIARSTYLIEDLFEMLPV
                     HWMWWPAIGGLAVGIVGLISPHTMGVGYDNIEHILSGNFLGTALLVFCVLKFISWSLA
                     LGSGTSGGTLAPIFTIGGGLGVLIGQGLNAAGLSVDVRMAGLVGMAACFAGASRALFA
                     SILFALEITGQFSTLLPLIAGCVAAFLVSAFLMRTTIMTEKLARRGMHVPSDYVATPP
                     AS"
     gene            complement(1499..>2776)
                     /gene="metE"
                     /locus_tag="VF402_10800"
     CDS             complement(1499..>2776)
                     /gene="metE"
                     /locus_tag="VF402_10800"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX7800831.1"
                     /translation="DLEDALDGDVKSWLAFSVQKMSELAAVAGALRDGRGSVKAALDS
                     AASAVATRRHSKLIHNPEVRARLAAVTEADARRHGAFAERAAVQRARFNLPSFPTTTI
                     GSFPQTPEVRQARSAHAKGDLSDAQYETFLREETARAIRWQEEIGLDVLVHGEFERND
                     MVQYFGEQLSGFAFTRHGWVQSYGSRCVRPPILFGDVSRPRPMTVAWQRYAQSLTNKP
                     VKGMLTGPVTILNWSFVRDDQPRSQSCRQIALAIRDEVADLEAAGARMIQIDEAALRE
                     GLPLRRRDWGGYLDWAVECFRLCASGVADETQIHTHMCYSEFNDIMDAIAAMDADVIS
                     IETSRSKMELLDAFGAKTYPNEIGPGVYDIHSPRVPAAAEMLELMTLARRRLSDAQLW
                     VNPDCGLKTRKWDEVRPALVNMVAAAKALREPA"
BASE COUNT          381 a         1009 c          905 g          481 t
ORIGIN      
        1 tgaatccccc cacacgcctc aggatatgta atgagttcct ccaatccgaa acacgatctg
       61 gaaaaagcgt cgaccacgcc cggcctgccc ctgtcgctgg gcatcggccc gctgatcgaa
      121 aaggccgatg tcgagcgcca cccgcgcgcc atcgatatgc gcagcctgat gatctgcgcg
      181 ctgtgcatgg tgctggccgc cgtggtcgcg ccggtcgcgc gccttctgat gctgctgatc
      241 ggcctgatca ccaatctggt ctttttcggg cgcgtgtcgg gcgagctggt ctcgcccgcg
      301 ggcagcccgc tgggcgtgtg gatcgtcgtc atgccggtga tcggctcgat catcgtcggc
      361 ctgatggccc gctacggcgc caaggccatc cgcggccacg gcatccccga ggcgatgtag
      421 cagatcctga ccaatgacag ccgcattccc gtgcgcatga ccttcctgaa gcccctgtcc
      481 gccggcgccg ccatcggcac cggcgggccg ttcggcgccg aaggcccgat catcgccacc
      541 ggcggcgcgc tcggctcgct gctgggccag ttgatccgcg tcagcgcgca cgaacgcaag
      601 gtgctgctgg cggcgggcgc ggcggcgggc atgaccgcca tcttcgccac gccgttctcg
      661 gccatcctgc tggccatcga gctgctggtg ttcgagttcc gtccgcgctc cttcatcccc
      721 atcgcgctgg cggcgctgac cgccatctgg ctgcgcccgt ggctgttcgg cgtgcatccg
      781 gtgttcgccg tgccgctgct gcccgccgcg tcgcagaccg cgctgatcgg ctatgtgctg
      841 gagggcctgt tcatggtcgt gctggcggtc ggcatcgcgc gctcgaccta cctgatcgag
      901 gatctgttcg aaatgctgcc cgtgcattgg atgtggtggc ccgccatcgg cggcctcgcg
      961 gtcggcatcg tcggcctcat ctcgccgcac accatgggcg tcggctacga caatatcgaa
     1021 catatcctga gcggcaattt cctcggcacg gcgctgctgg tgttctgcgt gctgaaattc
     1081 atctcgtggt cgctggcgct cggcagcggc acgtcgggcg gcaccctggc gccgatcttc
     1141 accatcggcg ggggcctcgg cgtgctgatc ggtcaggggc tgaacgccgc gggcctcagc
     1201 gtcgacgtgc gcatggcggg cctcgtcggc atggcggcct gcttcgcggg cgcctcgcgc
     1261 gcgctgttcg cctcgatcct gttcgcgctg gagatcaccg gccagttcag caccctgctg
     1321 ccgctgatcg ccggttgcgt cgccgccttc ctcgtctcgg ccttcctgat gcgcaccacc
     1381 atcatgaccg agaagctggc gcggcgcggc atgcacgtgc cgtcggacta cgtggccacg
     1441 ccgcccgcct cgtaaaacga cagggcgcgg cgatgacctt cgccgcgccc ttaacgtttt
     1501 acgccggttc gcgcagcgcc ttcgccgccg cgaccatgtt gaccagggcc ggccgcacct
     1561 cgtcccattt ccgggtcttc agcccgcagt ccgggttgac ccagagctgc gcgtcgctga
     1621 gccgccgccg cgccagcgtc atcagctcca gcatctccgc cgccgccggc acgcgcggcg
     1681 aatggatgtc atagacgccc ggcccgatct cgttcggata ggtcttcgcg ccgaacgcgt
     1741 ccagcaactc catcttcgag cgcgacgtct cgatggagat gacgtcggcg tccatggccg
     1801 cgatcgcgtc catgatgtcg ttgaactccg aatagcacat gtgggtgtgg atctgcgtct
     1861 cgtccgccac gcccgacgcg cacagccgga agcactccac cgcccaatcg agatagccgc
     1921 cccagtcgcg gcggcgcagc ggcagccctt cgcgcagggc cgcctcgtcg atctggatca
     1981 tgcgcgcgcc cgccgcttcg aggtcggcga cctcgtcgcg gatggccagc gcgatctggc
     2041 ggcagctttg cgaacgcggc tggtcgtcgc gcacgaacga ccagttgaga atggtcaccg
     2101 gcccggtcag catccccttg accggcttat tcgtcagcga ctgcgcatag cgctgccacg
     2161 ccaccgtcat cggcctgggg cgcgacacgt cgccgaacag gatcggcggc cgcacgcagc
     2221 gcgagccgta ggactgcacc cagccgtgcc tggtgaaggc gaagcccgac aactgctcgc
     2281 cgaaatactg caccatgtcg ttgcgctcga actcgccatg caccagcacg tcgaggccga
     2341 tctcctcctg ccagcggatc gcgcgcgccg tctcctcgcg caggaaggtc tcgtactgcg
     2401 cgtccgacag gtcgcctttg gcgtgggccg agcgcgcctg acgcacttcc ggcgtctgcg
     2461 ggaaggagcc gatggtggtc gtcggaaagg aaggcaggtt gaagcgcgcg cgctgcacgg
     2521 cggcgcgctc ggcgaaggcg ccgtggcggc gggcgtcggc ctcggtgacc gccgccagac
     2581 gcgcgcggac ctccgggttg tggatcagct tcgaatgacg ccgcgtcgcg accgccgagg
     2641 ccgccgaatc cagcgccgcc ttcacgctgc cgcgcccgtc gcgcaaggcg cccgcgaccg
     2701 ccgccagctc gctcatcttc tgcaccgaaa aggccagcca cgacttcacg tcgccgtcca
     2761 gcgcgtcctc gaggtc
//
LOCUS       DASVLL010000656         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_476132,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000656 DASVLL010000000
VERSION     DASVLL010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476132"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VF402_10805"
     CDS             <1..788
                     /locus_tag="VF402_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX7800832.1"
                     /translation="GTGANLVRLELRADTSAHDLRRALNRAARLKTPVMVMLWEQDVT
                     CAHDGARLRQYARDLWMGRWSHVLLDPKYQPYLMLNIANEWGTSADGFGDYMATYREL
                     IRALRGRGYRMPLVIDAADCGQNADSFTAGRGAALEAEDPLRNVIFSVHAYNRPWSDQ
                     AKIDANLAALKASGLPFLLGEFGSSQLVEDGYSVDHLYLMRRAQAGGIGWIAWSWKGN
                     GGPSRVLDMSRAYARADLTAHGRDVIDGPDGIRATAKTDLTTD"
     gene            877..>1779
                     /locus_tag="VF402_10810"
     CDS             877..>1779
                     /locus_tag="VF402_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX7800833.1"
                     /translation="MKKTSGELKLLAPMAMIVVVAVCALVGSLWVWAEQADTMSRVRE
                     EALVRIGLDQIADSHAQLMTPITIWDASVQNTAVAYNPAWVENNIGLYFRDFLNFENS
                     LLLDGRDHVTAAFRDGKPVDAAAMPGLRAQTAALVNGLRRTYERRRSQGLPYNADSNH
                     AGRLMAFNGHVYIVGASLLLPDSRSAKLLAYRPFAAVGMHELSDEELVTLSQRYMLSD
                     VRLLKPGQARAPGAASIAFRETDGKGPALAELSWTPDTPGHTLFIRALGPVLMISMGL
                     GLIAIVLLHQSQKAAQGLIASEAKA"
BASE COUNT          312 a          585 c          596 g          286 t
ORIGIN      
        1 cgggcacggg cgcgaacctc gtccggctgg agctgcgcgc cgacacgtcg gcgcatgacc
       61 tgcgccgcgc cctgaaccgg gccgcgcgct tgaaaacgcc ggtcatggtc atgctgtggg
      121 agcaggacgt gacgtgcgcg catgacggcg cgcggctgcg tcagtatgcg cgcgacctgt
      181 ggatgggccg ctggagccat gtgctgctcg atccgaaata tcagccgtac ctgatgctca
      241 acatcgccaa cgaatggggg acgtccgccg acgggttcgg cgactacatg gcgacctatc
      301 gcgaactgat ccgcgccctg cgcggacgcg gctaccgcat gccgctggtg atcgacgccg
      361 ccgattgcgg ccagaacgcg gacagcttca ccgccgggcg cggcgccgcg ctggaggccg
      421 aagacccgct gcgcaacgtg atcttcagcg tccacgccta taacaggccg tggagcgatc
      481 aggcgaagat cgacgccaac ctcgccgcgc tcaaggccag cggcctgccc ttcctgctcg
      541 gcgagttcgg ctccagccag ttggtcgagg acggctacag cgtcgatcac ctctacctga
      601 tgcggcgcgc gcaggccggc gggatcggct ggatcgcgtg gagctggaag ggcaatggcg
      661 gtccttcgcg cgtcctcgac atgtcgcgcg cctacgcccg cgccgacctg accgcgcacg
      721 gccgggacgt gatcgacggt cccgacggca tccgggcgac ggcgaaaaca gacttaacaa
      781 cagattaaat attaaaagtc agtcatttgc cgtaatttaa gacttatcca ctacagtgac
      841 gtctcaatca aggcaaagcc tcagaacgag actctggtga agaagacttc gggcgaatta
      901 aaactgctgg cgcccatggc gatgattgtc gtcgtggcgg tttgcgcgct ggtgggcagt
      961 ctttgggtat gggccgagca ggccgatacg atgtcgcgcg tccgtgagga agctttggtg
     1021 cgcatcggac tggatcagat cgccgacagc cacgcccagt tgatgacgcc gatcaccatc
     1081 tgggacgcct cggtccagaa tacggcggtc gcctataacc ccgcctgggt cgagaacaat
     1141 atcggcctct atttccgcga cttcctgaat ttcgagaaca gcctgctgct ggacggccgg
     1201 gaccatgtga cggcggcgtt ccgcgacggc aagccggtcg acgcggcggc catgccgggg
     1261 ctgcgcgcgc agacggcggc gctggtgaac ggcctgcgcc ggacctatga gcgccgccgc
     1321 agccagggcc tgccctacaa cgccgacagc aatcacgccg ggcggctgat ggcgttcaac
     1381 ggccatgtct atatcgtcgg cgcctcgctg ctgctgcccg acagccgcag cgcgaaactg
     1441 ctcgcctacc gcccgttcgc cgccgtcggg atgcacgaac tgtctgacga ggagctggtc
     1501 acgctgtcgc agcgctacat gctgtccgac gtccgcctgc tcaagccggg tcaggcgcgc
     1561 gcgccgggcg cggcctcgat cgcgttccgc gaaaccgacg gcaaaggacc ggcgttggcc
     1621 gagctgagct ggaccccgga cacgccgggc cacaccctgt tcatccgcgc gctggggccg
     1681 gtgctgatga tttcgatggg gctgggcctg atcgccatcg tgctgctgca ccagagccag
     1741 aaggcggcgc aggggctgat cgcgtccgag gccaaggcc
//
LOCUS       DASVLL010000657         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_421327,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000657 DASVLL010000000
VERSION     DASVLL010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421327"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VF402_10815"
     CDS             complement(<1..718)
                     /locus_tag="VF402_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800834.1"
                     /translation="MPSHSILRLAALVCGLAAAPVMAQAAAPTFAGLFGDHAVLQRDR
                     PAHVWGQAAPGAEVTLRLGAVSVTATAGADGGWQADLPAQGAGGPYVLSAADADGTTT
                     LNDVMLGDVYLCSGQSNMQFPVKYATNAWGEIYVAANPDLRFLNLDNASAPTPQAALM
                     KPAAWKIAGPQTSGDASAVCYYMSQAIQKEQGVAVGFIDSYWGGTTAQAWISADALKR
                     IDYKGHIEPSPWAPHDLSSLY"
     gene            1021..2076
                     /locus_tag="VF402_10820"
     CDS             1021..2076
                     /locus_tag="VF402_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7800835.1"
                     /translation="MARKLGRRSGNIVTIHDVARHVGVSPMTVSRVINGEKNVREETR
                     AKVMASVEALNYSPNLAARSLASQDSARIGLLYSNPSLAFLSEFLLGILSESAQSGVQ
                     LLIESCDGPEGATAALEKMKASGCDGVILSPPLSDSTRVAATLNQMKLPFVGVAMARP
                     ADDVNAVTIDDFKAAKAMTRRLIELGHQRIAFIKGHPTAYASQTRYEGFEAAMRKAGL
                     GVDPVFVAQGFFTYRSGFDAAERLLGQKERPTAIFASNDDMAAGAVAAAHRLGLDVPR
                     DVTIVGFDDTAMASSVWPELTTVRQPIADMAGMAVRLLTDAIRAHRAGKPFEPVREIR
                     DFSLIERQSSGAAADAG"
     gene            2255..>2947
                     /locus_tag="VF402_10825"
     CDS             2255..>2947
                     /locus_tag="VF402_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HEX7800836.1"
                     /translation="MPANETLTFLPQDWRDATLIGRVELPEGPTPIMVKGGEAFDMTP
                     VAPTVSQLLAAWPEKGVPSGGRSLGRLEDFHFGKLWSGARSGAQLLSPIDLQCVKAAG
                     VTFAVSAVERVIEERARGDADKAQAIRNALAERVGSDIRSVKPGSAEAARLKDALIAD
                     GLWSQYLEVAIGPDAEVFTKSPVLSSVGWGEEVGVRSDSTWNNPEPEVVMVVTSEGRA
                     VGATLGNDVNLRC"
BASE COUNT          473 a          979 c         1017 g          478 t
ORIGIN      
        1 tatagagcga ggacagatcg tgcggcgccc acggactcgg ctcgatatgg cccttgtaat
       61 cgatgcgctt cagcgcgtcg gcgctgatcc acgcctgcgc ggtggtgccg ccccagtagg
      121 aatcgatgaa cccgaccgcg acgccctgct ccttctggat cgcctggctc atatagtagc
      181 agacggcgga ggcgtcgccc gaggtctggg gtccggcgat cttccacgcc gccggtttca
      241 tcagcgcggc ctggggcgtc ggcgcgctgg cgttgtcgag gttcaggaag cgcaggtcgg
      301 gattggccgc gacgtagatc tcgccccagg cgttggtggc gtacttgacc gggaactgca
      361 tgttcgactg gccggagcag agatagacgt cgccaagcat gacgtcgttc agcgtcgtcg
      421 ttccgtccgc gtcggcggcg ctcaggacgt aaggcccgcc tgcgccttgc gcgggcaggt
      481 cggcctgcca gccgccgtcc gcgcccgccg tcgccgtgac gctgaccgcg ccgagcctca
      541 acgtcacctc ggcgcccggc gccgcctgcc cccagacgtg cgccggacgg tcgcgttgca
      601 gcacggcgtg atcgccgaac aggcccgcga aggtcggcgc cgccgcctgc gccatgaccg
      661 gggccgcggc gaggccgcaa acgagcgccg cgaggcgaag gatggaatgt gacggcatcg
      721 ggtctctccg ttgcggcgaa tcgctcaaaa cgttcgcgtc cgcgccttct tatatttgat
      781 tactttaatg ccggacgcgc aaaacgccgc gccgcgccgg tcataacggc gccgtctcca
      841 cggattatca agcttttttc cgcgcatccc ccgccgccgc cgtttttcgc gcagaaacgg
      901 attttcactt gcgaaacgcc tgtcgcgggt cgggaattgc caagcgccat gcgccgtcct
      961 atacactggg gcgaaacgat aaggcggccc aaggcaggct gcgtgaaata ggggcagtgc
     1021 gtggcgcgca aactgggtcg tcgaagcgga aacatcgtca ccatccatga cgtcgcgcgc
     1081 catgtcggcg tgtcgccgat gaccgtgtcg cgcgtcatca acggcgagaa gaacgtgcgc
     1141 gaggagacgc gcgccaaggt gatggcctcg gtcgaggcgc tcaactattc gcccaatctg
     1201 gcggcgcgtt cgctggcgtc gcaggattcg gcgcgcatcg ggcttttgta ctccaacccg
     1261 tcgctggcct ttctgagcga attcctgctc ggcatcctgt cggaaagcgc gcagagcggc
     1321 gtgcagttgc tcatcgaatc gtgcgacggc cccgaaggcg cgacggcggc gctggagaag
     1381 atgaaggcgt cgggctgcga cggcgtgatc ctgtcgccgc ctttgtcgga ttcgacgcgc
     1441 gtggccgcca cgctgaacca gatgaagctg cctttcgtcg gcgtggcgat ggcgcgcccg
     1501 gcggacgacg tcaacgccgt caccatcgac gatttcaagg ccgccaaggc gatgacgcgc
     1561 cgcctgatcg agctgggcca tcagcgcatc gccttcatca agggccatcc gaccgcctac
     1621 gccagccaga cgcgctatga ggggttcgag gcggcgatgc gcaaggcggg gctgggcgtc
     1681 gacccggtct tcgtggcgca gggcttcttc acctaccgtt ccggcttcga cgccgccgag
     1741 cgcctgctgg gccagaaaga gcgcccgacg gcgatcttcg cctccaatga cgacatggcc
     1801 gccggcgcgg tcgccgccgc gcaccggctg ggcctcgacg tgccgcgcga cgtcaccatc
     1861 gtcggcttcg acgacacggc gatggcctcg tcggtgtggc cggaactgac cacggtgcgc
     1921 cagccgatcg ccgacatggc cggcatggcg gtgcgcctgc tgaccgacgc cattcgcgcc
     1981 caccgcgccg gcaaaccgtt cgagccggtg cgcgaaatcc gcgatttttc cctcatcgag
     2041 cgccagtcct ccggagcggc tgcggatgcg gggtgaatcg cggcggcggc tgagatattt
     2101 cgcttgactc ctttggccat ctgcaagatt ggtagcgcta acacaaatca gacgcccgcg
     2161 gaccaccaga cgggccgaat caggttgaga cgatacggcg ccgcagggcg cgcactttga
     2221 agaagacacc cttgctgtaa ggacgacgac cgccatgccc gcaaacgaga ccctgacctt
     2281 cctgccgcag gactggcgcg acgccaccct gatcggccgc gtcgaactgc cggaaggccc
     2341 gacgccgatc atggtgaagg gcggcgaggc gttcgacatg acgccggtgg cgccgaccgt
     2401 gtcgcaactg ctggccgcgt ggccggaaaa gggcgttccg tccggcgggc gcagcctcgg
     2461 caggctggag gacttccatt tcggcaagct gtggtcgggc gcgcgttcgg gcgcgcagct
     2521 tctgtcgccg atcgacctgc aatgcgtgaa ggcggcgggc gtgaccttcg ccgtctcggc
     2581 ggtcgagcgc gtgatcgagg agcgcgcgcg cggcgacgcc gacaaggcgc aggcgatccg
     2641 caacgcgctg gccgagcgcg tcggctcgga catccgctcg gtcaagccgg gttcggccga
     2701 ggcggccagg ctgaaggacg ccctgatcgc cgacggcctg tggtcgcaat atctcgaagt
     2761 cgccatcggc ccggacgccg aggtgttcac caagtcgccg gtgctgtcca gcgtcggctg
     2821 gggcgaggag gtcggggtgc gctccgattc gacctggaac aatcctgagc cggaagtggt
     2881 gatggtggtg acgtccgaag gccgcgccgt cggcgccacg ctgggcaacg acgtcaactt
     2941 acgctgc
//
LOCUS       DASVLL010000658         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_260889,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000658 DASVLL010000000
VERSION     DASVLL010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260889"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VF402_10830"
     CDS             complement(<1..728)
                     /locus_tag="VF402_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX7800837.1"
                     /translation="MPRKVIIDTDPGQDDAVALLLALASPQDLDVLGVIATAGNVSLR
                     HTLNNARRVVELSGRTDVPVFAGSARPMRRALVTAEQVHGVTGLDGVDLPPPSLPEQA
                     QGGVDFLIERLRAAEDGEITLVTLGALTDLGHAFTRAPDVVRKVREVVLMGGSWREGG
                     NITPAAEFNIFVDPDAADIVFSSGVPLVMLPLDATHGVRSTPERMAALRALPNRCGPM
                     AARMLKASEAWDLAKFGWDGAPLH"
     gene            complement(936..>1733)
                     /gene="dinB"
                     /locus_tag="VF402_10835"
     CDS             complement(936..>1733)
                     /gene="dinB"
                     /locus_tag="VF402_10835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7800838.1"
                     /translation="FAAYTPLIEPLSLDEAYLDVTENLPGMASATDIAREIRARIREA
                     TGLTASAGVSYNKFLAKLASDQNKPDGLCVIRPEQGEAFVAGLPVKKFHGVGPATAAK
                     MQRLGIATGADLRARPLEELTRAFGVSGQHYYWIARGVDRREVRPDRARKSIGAETTF
                     FTDLTAFDAARNEMTPLIDKVWRYAESNGVKARTITLKVKFADFRIITRSQTGAVLTG
                     KDGLTAAVDGLLRGVFPPSKGIRLLGVTLSNLNAPDEDEAGQLSLGL"
BASE COUNT          287 a          588 c          576 g          282 t
ORIGIN      
        1 tcgtgcagcg gcgcgccgtc ccagccgaac ttggccagat cccacgcctc cgacgccttc
       61 agcatccgcg ccgccattgg cccgcagcgg ttgggcaggg cgcgcaaggc ggccatgcgc
      121 tccggcgtcg agcgcacgcc gtgcgtggcg tccagcggca gcatgaccag cggaacgccg
      181 ctgctgaaca cgatgtcggc ggcgtcggga tcgacgaaga tgttgaactc ggcggcgggc
      241 gtgatgttgc cgccttcgcg ccatgaaccg cccatcagca cgacctcgcg caccttcctc
      301 accacgtccg gcgcgcgcgt gaaggcgtgg ccgagatcgg tcagcgcgcc cagcgtcacc
      361 agcgtgatct cgccgtcttc agcggcgcgc aggcgctcga tcaggaaatc gacgccgccc
      421 tgcgcctgct ccggcaggga cggcggcggc aggtcgacgc cgtcgaggcc ggtgacgcca
      481 tgcacctgct cggcggtgac cagggcgcgc cgcatcggcc gcgccgaacc ggcgaacacc
      541 ggcacgtccg tgcggcccga cagctcgacc acgcggcgtg cattgttgag cgtatgacgc
      601 agcgaaacat tgcccgccgt cgcgatcacg cccagcacgt cgagatcctg cggcgaggcc
      661 aacgccagca aaagcgcgac ggcgtcgtcc tgaccgggat cggtgtcgat gatgacttta
      721 cgcggcaaac gtccggctcc ttggcgaggg gggagcggag actaggcgca ttttggttgc
      781 gggggaaagg gatttttcgc cacgaaaggc acgaaagcac acgaaaaata ggcgaagccg
      841 aaaagccctg ttctattccc tgtcgtcaaa gagaatggcg cgcttcgcgc aagattcttt
      901 ttcgtgtgtt ttcgttcctt tcgtggcgaa aaaccctaca atcccaaact caactgcccc
      961 gcctcgtcct catcgggcgc gttcagattg gacaaggtca cccccagcag cctgatccct
     1021 ttcgacggcg gaaacacccc gcgcagcagg ccgtcgaccg ccgcggtcag gccgtctttc
     1081 ccggtcagaa ccgcgccggt ttgcgagcgg gtgatgatgc ggaaatcggc gaacttgacc
     1141 ttgagcgtga tggtgcgcgc cttgacgccg ttgctctcgg cgtagcgcca caccttgtcg
     1201 atcagcggcg tcatctcgtt ccgcgccgcg tcgaaggcgg tcaggtcggt gaagaaggtc
     1261 gtctcggcgc cgatggactt gcgcgcgcgg tcggggcgca cctcgcgccg gtcgacgccg
     1321 cgcgcaatcc agtaatagtg ctggcccgac acgccgaagg cccgcgtcag ttcctccagc
     1381 ggccgggcgc gcaaatccgc gccggttgcg atgccgaggc gctgcatctt cgccgccgtg
     1441 gccgggccga cgccgtggaa ctttttcacc ggcaggcccg cgacgaaggc ttcaccctgt
     1501 tcgggccgga tcacgcacag gccgtccggc ttgttctggt ccgaggccag cttagccagg
     1561 aacttgttgt aggacacgcc cgccgaggcg gtcagcccgg tcgcctcgcg gatgcgggcg
     1621 cggatttccc tggcgatgtc ggtggccgag gccatgcccg gcaggttctc cgtcacgtcg
     1681 agataggctt cgtccagcga caaaggctcg atcagcggcg tataggccgc gaa
//
LOCUS       DASVLL010000659         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_156663,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000659 DASVLL010000000
VERSION     DASVLL010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156663"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1156)
                     /locus_tag="VF402_10840"
     CDS             complement(26..1156)
                     /locus_tag="VF402_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7800839.1"
                     /translation="MNASFRRVMAILIKEFIQMRRDRLTFAMMLVIPVVQLALFGFAI
                     NSDPRHLPTLLYMEEQSALTRSIVAGMENSTYFDIIGSVPSAEAGRKALQSGEAAFVL
                     TVPAGFTRGVERGDRPQLLLEADASDPSAASNAVGMFGQIVSSALSHDLKGQWAPLDQ
                     GPPPVDVVVHRMYNPEGVSQYNIVPGLLGVILTMTMIMITSVAMTRETERGTMENLLA
                     MPARPWEVMAGKILPYIGIGVVQTLIVLGAAAWVFHVPFIGDPWLLALGVALFIVANL
                     AVGFTFSTIARSQMQAMQMTFFFFLPSILLSGFMFPFRGMPQWAQWIGQIFPLTHFLR
                     VIRGVMLKGADFAAMWPSIWPLLLFIAVATAIAMARYRSTLD"
     gene            complement(1153..>1858)
                     /locus_tag="VF402_10845"
     CDS             complement(1153..>1858)
                     /locus_tag="VF402_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7800840.1"
                     /translation="KDRVGYMTQKFSYWEDLTIRENLEFVANLYGVADRKAVVDRTLE
                     TLGLASRQNQLAGALSGGWKQRLALAACTLHEPQMLMLDEPTAGVDPQARRDFWDQIH
                     RLSSEGLTVLVSTHYMDEAERCDQIVYIAYGDLMAQGRVADIIAQSGLFTFVAEGPGV
                     RDLMTRLRVKPGADHVAYFGNALHVTGSDPDKLKALIAQDGDKTGVTWRQEPPNLEDV
                     FIHLMDRSVDKAGIDK"
BASE COUNT          346 a          596 c          615 g          301 t
ORIGIN      
        1 gggaacccct tttggagaaa cgctcttaat ccagcgtcga gcggtagcgc gccatcgcta
       61 tggccgtggc gaccgcgatg aagagcagca gcggccagat gctcggccac atggcggcga
      121 agtcggcgcc tttcagcatg acgccgcgga tgacgcgcag gaaatgggtg agcggaaaga
      181 tctggccgat ccattgcgcc cattgcggca tgccgcggaa cgggaacatg aagcccgaca
      241 gcaggatcga cggcaggaag aagaagaagg tcatctgcat ggcctgcatc tgcgagcggg
      301 ctatggtcga gaaggtgaag ccgaccgcca gattggcgac gatgaacagg gccacgccca
      361 gcgccagcag ccacggatcg ccgatgaacg gcacatggaa cacccacgcc gccgcgccca
      421 gcacgatcag ggtctgcacc acgccgatgc cgatataggg caggatcttg cccgccatca
      481 cctcccacgg gcgcgcgggc atggccagaa ggttctccat cgtgccgcgc tcggtctcgc
      541 gcgtcatggc gaccgaggtg atcatgatca tggtcatggt caggatgacg cccagaaggc
      601 ccggcacgat attgtactgc gacacgcctt cgggattgta catgcgatgg acgacgacat
      661 cgaccggcgg cggcccctga tcgagcggcg cccactggcc tttcaggtca tggcttaaag
      721 cggaggaaac gatctggccg aacatgccga ccgcgttgga ggccgccgac gggtccgagg
      781 cgtcggcttc cagcagcagt tggggccggt cgccgcgctc cacgccgcgc gtgaagcccg
      841 ccgggaccgt cagcacgaag gccgcctcgc ccgattgcag cgccttgcgc ccggcctcgg
      901 cgctggggac cgagccgatg atgtcgaaat aggtcgagtt ttccatgccc gcgacgatgg
      961 agcgcgtcag ggccgattgc tcctccatgt agagcagcgt cggcaggtgg cgcggatcgg
     1021 aattgatcgc gaagccgaac agcgccagtt gcaccaccgg aatgaccagc atcatcgcga
     1081 aggtcagccg gtcgcggcgc atctggatga attccttgat caggatcgcc atcacgcgtc
     1141 ggaaggaggc gttcatttgt cgatccccgc cttatcgacg gacctgtcca tcaggtgaat
     1201 gaacacgtcc tcgagattgg gcggctcctg ccgccaggtc acgccggtct tgtcgccgtc
     1261 ctgcgcgatc agcgccttca gcttatcggg atccgagccg gtgacgtgca aggcgttgcc
     1321 gaaataggcg acgtggtcgg cgcccggctt aacccgcagg cgcgtcatca ggtcgcgcac
     1381 gcccggccct tcggcgacga aggtgaacag gccgctctgc gcgatgatgt cggccacccg
     1441 gccctgcgcc atcaggtcgc cataggcgat atagacgatc tggtcgcagc gttcggcctc
     1501 gtccatgtaa tgggtcgaga ccagcacggt cagcccttcg gacgacaggc ggtgaatctg
     1561 gtcccagaag tcgcggcgcg cctgcggatc gaccccggcg gtcggctcgt ccagcatcag
     1621 catctgcggc tcgtgcagcg tgcaggcggc cagggccagc cgctgcttcc agccgccgga
     1681 caaggcgccc gccaactggt tctggcgcga ggcgaggccc agcgtctcca gcgtccggtc
     1741 gaccaccgcc ttcctgtcgg cgacgccgta gagattggcg acgaattcga ggttttcgcg
     1801 gatcgtcagg tcttcccaat aggaaaactt ctgcgtcatg tagccgacgc ggtccttg
//
LOCUS       DASVLL010000660         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_315359,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000660 DASVLL010000000
VERSION     DASVLL010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315359"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF402_10850"
     CDS             complement(<1..552)
                     /locus_tag="VF402_10850"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX7800841.1"
                     /translation="MVRAVAEIGGFGRDAARRFGDEIVAACRPVVLRGLVADWPATQA
                     RSLHAYLAPMAADRSTEIFVGAPAIAGKYYYGEGLNGLNFTRRQAALAEALRLALTPR
                     ADAAAPSVYAGSLPADDLFPAFAAANPMPLAGGSPRLWLGHASNVSPHYDGYDNLACV
                     IAGRRRFTLYAPELTGRLYPGPID"
     gene            complement(545..1270)
                     /locus_tag="VF402_10855"
     CDS             complement(545..1270)
                     /locus_tag="VF402_10855"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX7800842.1"
                     /translation="MPNIAILNSQTHRDLRVRARAPDQPEDRLNFVPVVVGEFPHLAA
                     HYPILLSKDADTGAFYAGAMLGFEAGENLFIDESGNRDLYRPLNLQRVPFFTSGEHLA
                     IDLDSPRVGAHADGQRLFDDDGQPSAYLQAVTGVFQQLVPGLERTRGFIARLLELRLI
                     EPVTFDLAFDDGRTIATEGLYTPDQDALRALPDEAVVRLFRDGSLHLISLMIASLKQM
                     PQLARRKNERLAQGASALADAFG"
     gene            complement(1279..2820)
                     /locus_tag="VF402_10860"
     CDS             complement(1279..2820)
                     /locus_tag="VF402_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7800843.1"
                     /translation="MSGQVQNIVIVGGGTAGWLTACIIAARHKARIAAGRFSVTLVES
                     PRIAPVGVGEGTWPTLRGTLEKIGVSETDFFRECDVAFKQGARFAKWTTGAGDDYYYH
                     PLMLPQSFMQMNLAPHWLRHGGGMSFCDAVSPQGQLCEAGLAPKTIATPEYQAVANYA
                     YHLDAGKFAPFLTRHGVEKLGVRHVRAEVNGVEQAANGDVLGLDTEAGRIAGDLFVDC
                     TGFHALLIGKTMGVPFRDCGDVLFCDTALAVQLPFEREDAPIASQTNATALKAGWVWD
                     IALPTRRGLGYVYSSRHTDEAAACDELAAYVGPRFKDLSVRKIPIRSGHRETFWTRNV
                     VAVGLAAGFLEPLEASAIVLVELSAKLIAEQTPANRQVMDIVARRFNETTHYRWGRII
                     DFLKLHYTLTQREDTAFWRDNRDPAGIPERLQDLMTLWKYQSPWFFDEFDRLEEVFPA
                     ASYQYVLYGMGFKTEVEDDAADDAVRARRLMGENLAVTRKLRGQLPNNRELIRKIHDF
                     GLQPV"
     gene            complement(3027..>4505)
                     /locus_tag="VF402_10865"
     CDS             complement(3027..>4505)
                     /locus_tag="VF402_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800844.1"
                     /translation="QHNHDVVSQAKFMGSWDEDNLHLKAGVQYVDETQDLANVGNFAN
                     GNWQAYAGYGPASNSTGGVPIPAKYITGSFSTSNFIHGFSGNNNLPANIPVYDGLGVF
                     NYLQSLSPACASGCDPHVNGPFMIALDPASVQEVQEKTLAGFVSLNTTLQLAGRALEV
                     NVGVREEDTHVTSDGIGQLPVSMSLQPHDPTAFNIVYTPTQPLSDKNHYRYMLPNLDL
                     NYALTDDVKLRFDASRTLTRPGLTAMTPDLNVGQGQRVNALTGSGGNPYLLPYLSDNL
                     DFGAEWYYSRNSYAAIDFFSKDVTNFIVTNTITQSINGVIDPTTNKLAQFAYSTSVNG
                     PSANVHGVELAWQHVFADTGFGFQANATFVGTDKPYDPSNITTSGFAVTGLANSANVV
                     GFYDKHGFQARIAVNWRDEYLDHFGQTQNVSQFGTEPTIVDASTNVDFSTSYDINRNL
                     SVFLEGLNLTNQTLTTHGRYADQVLDLYDYGQRWTLGVRYHY"
BASE COUNT          753 a         1437 c         1555 g          760 t
ORIGIN      
        1 gtcgatgggg ccgggataga gccggccggt cagctccggc gcatagaggg tgaagcgccg
       61 ccgcccggcg atcacgcagg ccagattgtc gtagccgtca tagtgcggcg acacgttcga
      121 ggcgtggccc agccacaggc gcggcgatcc gcccgccagc ggcatgggat tggcggcggc
      181 gaaggcggga aagagatcgt ccgcgggcag cgatccggca tagacgctgg gcgccgccgc
      241 gtccgcgcgc ggcgtcaggg ccagccgcag cgcctcggcc aaagcggcct gacggcgggt
      301 gaagttgagg ccgttcagcc cttcgccgta atagtacttc cccgcgatcg cgggcgcgcc
      361 gacgaagatt tccgtgctgc ggtccgccgc catcggcgcc agataggcgt gcaggctccg
      421 cgcctgcgtc gcgggccagt cggcgaccag accgcgcagc acgaccggac ggcacgcggc
      481 cacgatctcg tcgccgaagc gccgcgccgc gtcgcggccg aagccgccga tctccgcgac
      541 cgccctaacc aaacgcgtcc gccagcgccg acgcgccctg cgccagccgc tcgttcttgc
      601 ggcgcgccag ttgcggcatc tgcttcagcg aggcgatcat cagcgagatc agatgcagcg
      661 agccgtcgcg gaacagacgc acgaccgctt cgtccggcag ggcgcgcagc gcgtcctgat
      721 cgggcgtgta aagcccctcg gtcgcgatcg tgcgcccgtc gtcgaaggcc aggtcgaagg
      781 tcaccggctc gatcagcctg agttccagaa gccgcgcgat gaagccgcgc gtgcgctcaa
      841 ggccgggcac gagctgctgg aacacgcccg tgaccgcctg cagataggcg ctgggctggc
      901 cgtcgtcgtc gaacaggcgc tggccgtcgg cgtgggcgcc gacgcgtggg ctgtcgagat
      961 cgatggccag atgctcgccc gaggtgaaga agggcacgcg ttgcaggttc agcggccggt
     1021 agaggtcgcg gttgccgctc tcgtcgatga acaggttttc gcccgcctcg aagcccagca
     1081 tggccccggc gtagaaggcg cccgtatcgg cgtccttgga cagcaggatc ggataatggg
     1141 cggccagatg gggaaattcg ccgacgacca cgggcacgaa gttcagccgg tcctccggct
     1201 ggtccggcgc gcgggcgcgc acgcgcaggt cgcggtgggt ctggctgttg aggatggcga
     1261 tattgggcat gacgtctcct aaacaggttg caggccgaag tcgtgaatct tgcggatcag
     1321 ctcgcggttg ttcggcaact ggccgcgcag cttgcgcgtc acggccagat tctcgcccat
     1381 cagccgccgc gcgcgcacgg cgtcgtccgc cgcgtcgtcc tcgacctcgg tcttgaagcc
     1441 catgccgtac agcacgtact gatagctggc cgccgggaag acctcctcca gccggtcgaa
     1501 ctcgtcgaag aaccagggcg actggtattt ccacagcgtc atcagatcct gaagccgctc
     1561 gggaatcccg gccgggtcgc ggttgtcgcg ccagaaggcg gtgtcctcgc gctgcgtcag
     1621 cgtatagtgc agcttgagga agtcgatgat ccggccccag cggtaatggg tcgtctcgtt
     1681 gaagcgccgc gccacgatgt ccatgacctg acggttggcc ggagtctgtt cggcgatcag
     1741 cttggccgac agctcgacca gcacgatggc cgaggcttcc agcggctcca gaaatcccgc
     1801 cgccagcccg accgcgacga cattgcgcgt ccagaaggtc tcgcgatggc cggagcggat
     1861 cggaatcttg cgcacgctca gatccttgaa gcgcggcccg acataggcgg ccagctcgtc
     1921 gcaggcggcg gcctcgtccg tgtggcgcga ggaatagaca tagcccagcc cgcgccgcgt
     1981 cggcagggcg atgtcccaca cccagcccgc cttgagcgcg gtcgcgttgg tctgcgaggc
     2041 gatcggcgcg tcctcgcgct cgaacggcag ttgcacggcc agcgccgtgt cgcagaacag
     2101 cacgtcgccg caatcgcgga acggcacgcc catcgtcttg ccgatcagca gggcgtgaaa
     2161 gccggtgcag tccacgaaca ggtcgcccgc gatccggccc gcctcggtgt cgaggccgag
     2221 gacgtcgccg ttcgcggcct gctcgacgcc gttcacctcg gcgcgcacat ggcgcacgcc
     2281 cagcttttcg acgccgtggc gcgtcaggaa gggcgcgaac ttgcccgcgt cgaggtgata
     2341 ggcgtaattg gccaccgcct gatattcggg cgtggcgatg gtcttgggcg ccagccccgc
     2401 ctcgcacaac tggccctgcg gcgacaccgc gtcgcaaaag ctcatccccc cgccgtggcg
     2461 cagccagtgc ggcgccagat tcatctgcat gaagctctgc ggcagcatga gcggatgata
     2521 ataataatcg tccccggcgc cggtcgtcca tttggcgaac cgggcgccct gcttgaaggc
     2581 gacgtcgcat tcgcggaaga agtccgtctc cgacacgccg atcttttcca gcgtgccgcg
     2641 cagggtcggc catgtgccct cgcccacgcc gaccggggcg atgcggggcg attccaccag
     2701 ggttacgctg aaccggcccg ccgcgatgcg cgccttgtgc cgcgcggcga tgatgcaggc
     2761 cgtcagccag cccgccgtgc cgccgccgac gataacgata ttctgaacct gaccggacat
     2821 gagatgcgtc caaagaaaag aaaaagacgg accgccgcag acgtggcgcg gcggtcccag
     2881 ggaaagaaga taccacactt tcagaggacg ctaagccgcg ccctcaaggc cgtacacaca
     2941 catcgtcagg cgcaggcccg gccgctcagg accggacccg aaggcggctc ccgtacccgc
     3001 cgtggcgcgc cgggagccgt ccggggtcag tagtggtagc gcacgcccag cgtccagcgc
     3061 tggccgtagt cgtacaggtc gagaacctga tcggcgtaac ggccatgcgt ggtcagggtc
     3121 tggttggtca ggttcagacc ttccaggaac acgctgagat tccggttgat gtcgtagctg
     3181 gtcgagaagt cgacattggt gctggcgtcc acgatggtcg gctcggtgcc gaattgcgac
     3241 acattctggg tctggccgaa gtgatcgaga tattcgtcac gccagttgac cgcgatacgg
     3301 gcctggaagc cgtgcttgtc gtagaaaccg accacgttgg ccgaattggc cagcccggtc
     3361 accgcaaagc ccgacgtcgt gatattgctc gggtcgtacg gtttgtcggt gccgacgaag
     3421 gtggcgttgg cctggaagcc gaagccggtg tcggcgaaga cgtgctgcca cgccagttcg
     3481 acgccgtgga cgttggcgga cgggccgttc accgacgtgc tgtaggcgaa ttgcgccagc
     3541 ttgttggtgg tcggatcgat cacgccgttg atcgactggg ttatcgtgtt ggtgacgatg
     3601 aagttggtca cgtccttgct gaagaaatcg atcgcggcgt aggagttgcg gctgtaatac
     3661 cactcggcgc cgaaatcgag attgtccgac agatagggca gcaggtaagg attgccgccg
     3721 ctgccggtca gggcgttgac gcgttgtccc tgaccgacgt tcaggtcggg cgtcatcgcc
     3781 gtcaggcccg gacgggtcag ggtgcgcgaa gcgtcgaagc gcagcttgac gtcgtcggtc
     3841 agggcgtagt tcagatccag attcggcagc atgtaccggt aatggttctt gtccgacagg
     3901 ggctgggtcg gcgtatagac gatgttgaac gccgtgggat cgtgcggttg cagcgacatg
     3961 ctgaccggca attgcccgat gccgtccgag gtgacgtgcg tgtcttcctc gcggacgccg
     4021 acattgacct ccagggcgcg gccggccagt tgcaaggtcg tgttcaggct gacgaagccc
     4081 gccagcgtct tttcctggac ttcctgcacg ctggccggat cgagggcgat catgaagggg
     4141 ccgttgacgt gcgggtcgca gccgctggcg caggccgggc tgaggctttg caggtagttg
     4201 aacacgccaa gcccgtcata gaccggtatg ttggcgggca ggttgttgtt gccgctgaag
     4261 ccgtggatga agttcgacgt gctgaacgag ccggtgatat acttcgccgg gatcggcacg
     4321 ccgcccgtgc tgttcgacgc cgggccgtag ccggcataag cctgccagtt gccgttggcg
     4381 aagttgccga cattggccag atcctgggtt tcgtcgacat actggacgcc ggccttcagg
     4441 tgcaggttgt cctcgtccca actgcccatg aacttggcct gactgaccac gtcatgattg
     4501 tgctg
//
LOCUS       DASVLL010000661         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_367963,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000661 DASVLL010000000
VERSION     DASVLL010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367963"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VF402_10870"
     CDS             complement(<1..833)
                     /locus_tag="VF402_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7800845.1"
                     /translation="MLAVILGEGEAASAGFAKLDKALAADVLAQLAAVGFKGARGKTQ
                     LLGRPAGLAAAGLAVTGGGDLNALDAAAVETLAAGLYHAVKASGFEALSLDLSGFTPE
                     QVAQAYLGLHLASYRFDKYFGQSKLDKGPALKAITVVCDDPAAAEAAFAPLAGLRDGV
                     VFARDLVSEPANVLYPQSYADRLKELESIGLKVEILGVEEMRKLGMNALLGVGQGSAR
                     ESKLVVMQWRGADAAAAPVAFVGKGVTFDTGGISIKPAEGMEDMKWDMGGSAAVVGTM
                     IA"
     gene            1159..>1895
                     /locus_tag="VF402_10875"
     CDS             1159..>1895
                     /locus_tag="VF402_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7800846.1"
                     /translation="MRTLDRYIFRQTLTPVLGAVAALTAIALLSQSLSQFDLVVERGQ
                     STWTFLRITLLSLPQLAGMIFPIALFVGSLVALTRLQGEHEFTAAYASGMSLARIAYP
                     VVRIGVYFTLISLVANLFLQPLTARAMRHELFDIKNDLVATLVKEGEFSTSETGLTIY
                     VKRIDQNGLLREIFIRTPATGGHDRTYAAREGRIIKQDGLSYLVMRKGSTEQVNDQGA
                     LDHTTFDEYSIDITPYFATDDFLQFKIG"
BASE COUNT          333 a          663 c          581 g          318 t
ORIGIN      
        1 gcgatcatgg tcccgaccac ggcggccgag ccgcccatgt cccacttcat gtcctccatg
       61 ccctcggcgg gcttgatgga gatgccgccg gtgtcgaagg tcacgccctt gccgacgaag
      121 gccaccggcg cggcggcggc gtccgcgccg cgccactgca tgacgaccag cttcgattcg
      181 cgcgcgctgc cctgccccac gcccagaagc gcgttcatgc ccagcttgcg catctcttcg
      241 acgcccagaa tctcgacctt gaggccgatg ctttccagtt ccttcaggcg gtcggcgtag
      301 gattgcggat acagcacatt ggccggttcc gacaccagat cgcgcgcgaa cacgacgccg
      361 tcgcgtaagc ccgccagcgg cgcgaaggcg gcctcggcgg cggcgggatc gtcgcagacc
      421 acggtgatcg ccttgagcgc cggccccttg tccagcttgg attggccgaa atatttatcg
      481 aaacgatagc tggccagatg caggccgaga taggcctgcg cgacctgctc cggcgtgaag
      541 ccgctcagat cgaggctcag cgcctcgaaa cccgacgcct tgaccgcgtg ataaagcccg
      601 gcggccagcg tctcgacggc cgcggcgtcg agggcgttca gatcgccgcc gcccgtcacc
      661 gcaaggcccg ccgccgcgag gcccgccgga cgccccagaa gctgcgtctt gccgcgcgcg
      721 cccttgaagc ccaccgcggc cagttgcgcc agaacgtcgg cggccagcgc cttgtccagt
      781 ttcgcaaagc ccgcgctggc cgcctcgccc tcgcccagga tgacggccag caaggatttt
      841 tcggcgggtg cggcggcaag gcggagcgtt ggcgacggca tggtgatgaa ctcccgaaca
      901 gaagggcgac aaagattttc cgcctattag ctaggcgacg gctgtttctt ttgcaaacgt
      961 tttgcgcccc ggcgcccagg ctttttccgt cgcatccggc cggagagacg ccagagcgat
     1021 aaccagggac ataatcgggc ataaattttg tgcgccgcga cggcgcggct tgtaattaac
     1081 cgcgtaagcg gtcaaaaggg aggcggaaaa ccggcgccag ggccggaccc cgtcacctta
     1141 cacggcccgc cgacacgcat gcgcacctta gaccgctaca ttttccgcca gaccctgacg
     1201 ccggttctgg gcgccgtggc cgcgctgacg gcgatcgcgc tgctgagcca gtccctgtcg
     1261 cagttcgacc tggtggtcga gcgcggccag agcacctgga ccttcctgcg catcacgctt
     1321 ctgtcgctgc cgcaactggc ggggatgatc tttcccatcg ccctgttcgt cggcagcctg
     1381 gtcgcgctga cgcgtctgca gggcgaacac gaattcaccg ccgcctacgc cagcggcatg
     1441 tcgctggcgc gcatcgccta tccggtcgtg cgcatcggcg tctatttcac cctgatcagc
     1501 ctggtcgcga atctgttcct gcaaccgctg accgcgcgcg ccatgcggca cgaactgttc
     1561 gacatcaaga acgatctggt cgccacgctg gtgaaggaag gcgaattctc cacctcggaa
     1621 acgggcctga ccatctatgt caagcgcatc gaccagaacg gccttttgcg ggaaatcttc
     1681 atccgcaccc cggcgaccgg cgggcacgac cgcacctacg ccgcgcgcga aggccggatc
     1741 atcaagcagg acggcctgtc ctatctggtc atgcgcaagg gctcgaccga gcaggtcaac
     1801 gatcagggcg cgctggacca caccacgttc gacgaataca gcatcgacat caccccctat
     1861 ttcgccacgg acgatttcct gcagttcaag atcgg
//
LOCUS       DASVLL010000662         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_421941,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000662 DASVLL010000000
VERSION     DASVLL010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421941"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1270)
                     /locus_tag="VF402_10880"
     CDS             complement(323..1270)
                     /locus_tag="VF402_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7800847.1"
                     /translation="MRKAVAPVLMASTALLGAAPDMALAQSAKASKVQQQQDKMVASI
                     PHCTKRLGTITIVDGDDSNGWTQANLAPPQKLLKVIVQKSGCFGLVDRGAGMDAAARE
                     RALNQSMGLQHGSNVGQGQIKAADYVLVAEVGSADSNTGGGGAAGLIGGLVGGQVGGL
                     IGGFRTKKLESDVVLSITDVRTSESNSFEGHAAKNDLTWGAGGAIGFGGAVGGGYEDT
                     DIGRIVTQAFIVAYSDMVNNLGGIDAGGAAAAPSRSYTVQSATLLRKTPSPKGAVVRA
                     LPAGLVVYPTGNKDGMYWEVADDNDNVGWVSNDKLAASQ"
BASE COUNT          272 a          541 c          532 g          279 t
ORIGIN      
        1 gtcccagacg gtcgcgtcgg tcggaacctg attgggatcg tccttatcgt cgtcgccgcc
       61 gaaaatctgc gcgtgcgcgg cgggcgccgg taaaaggacc gttccggcgg cggtaaaacc
      121 gcccgtcagc agggtcaggc cgaggcccgc cataaggcgc gcgcgaaact tatgagaatg
      181 tcccggaaat ggtgtagccg acatgacgtt ccgccctgac tcctggaaaa caagggttca
      241 tccagctagc aacatagtca ggctgaaata agacgtaaag gccccttgcg ggcggcgttt
      301 ccgcccgcaa gggaaggccc gtttactggc tggcggccag cttgtcgttc gacacccagc
      361 cgacattgtc gttgtcgtcg gcgacttccc agtacatgcc gtccttgttg ccggtcggat
      421 agacgaccag ccccgccggc agggcgcgca ccaccgcgcc cttggggctc ggcgtcttgc
      481 gcagcagggt cgcggactgc accgtatagc tgcgcgacgg agccgccgcc gcgccgcccg
      541 cgtcgatgcc gcccagattg ttgaccatgt cgctgtaggc cacgatgaag gcctgcgtca
      601 cgatgcggcc gatatcggtg tcctcatagc cgccgccgac cgcgccgccg aagccgatcg
      661 cgccgcccgc gccccaggtc aggtcgttct tggccgcgtg gccctcgaac gagttggatt
      721 cggaggtgcg cacgtcggtg atcgacagca cgacgtcgga ttccagcttc ttggtgcgga
      781 agccgccgat caggccgccg acctgcccgc cgaccagacc gccgatcagg cccgccgcgc
      841 cgccgccgcc cgtgttggaa tcggcgctgc cgacctcggc caccagcacg tagtccgccg
      901 ccttgatctg gccctgaccg acgttcgagc cgtgttgcag gcccatcgac tggttcagcg
      961 cccgttcgcg cgccgccgcg tccatgcccg cgccgcggtc gacgaggccg aaacagcccg
     1021 acttctgcac gatgaccttg agcagctttt gcggcggggc caggttggcc tgcgtccagc
     1081 cgttggaatc gtcgccgtcg acgatggtga tcgtgcccag acgcttggtg cagtgcggga
     1141 tggacgcgac catcttgtcc tgctgctgct gaaccttgct ggccttggcc gattgcgcca
     1201 gcgccatgtc gggcgcggcg cccaggaggg cggtggacgc catcaggacg ggcgcgacgg
     1261 ctttacgcat atggaatgaa aacatgggtg gcctccgccg gaaaaggatt tcgaaacagc
     1321 ggaacggcgc aacctgttgt ttcccccgcc cgccgcgacg cgccgcctga gcgcccgacc
     1381 ggcgcgaacg aggacgaacg cgatgacgcg atacttaaaa agtgtccgcg cggcgcccgc
     1441 agggcaggcc acaaaaagaa cacactccac accttacggg tgcggagtgt gtaaggcaaa
     1501 tcgaaatttg cccgtttgcg aaggtttcgc gaaaaccgtt attgcgcgcc ggacaccagc
     1561 gtggtgtggg cgttgcggtt cttggcccag gcgtcttcgc cgtcgccgtt gtcgatcggc
     1621 tgtt
//
LOCUS       DASVLL010000663         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_368282,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000663 DASVLL010000000
VERSION     DASVLL010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368282"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VF402_10885"
     CDS             <1..1773
                     /locus_tag="VF402_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEX7800848.1"
                     /translation="DPRPKPAVTPRLQPAAYAQVRLAHGPMLSQRAHQHALYMGLDND
                     RLLKPFRVRAGLPAPGPDMGGWYDQSDDFVIDTHDWSKTNWHGYIPGHSFGQYLSGLA
                     RLYAVTGDAATRAKVASLVRDYAPTISPRFFDGYTLPAYTYDKIVIGLIDAYRYADVK
                     EAAGALDKVTDAALPYLPERALTREQRRKLPYASEAQIWDEPYTLPENLFKAWRLGLG
                     QRYYDLAVRYLQDEALFDPLAQGVSPLKGRHAYSHVNALSSAIEAYLATGDETYLRAA
                     ANGFAFIERQSFATGGWGPNETLIGPDDDDALLTSLSQTHSSFETPCGAYGHFKITRS
                     LLRLTGDSHYGDSMERVLYNTILGALPTTPEGKTFYYSDYNRTAGKFYRPELWPCCSG
                     TFIQLTADYGVSAYLSNARAVYVNLYLPSDLQTEIGGVGVRLRQTTDYPLADTVRIDV
                     QPERPARFTLALRMPAWAGPATRVTVNGEAVPVAPGRFLRLERLWRAGDAVEIRFDMG
                     LRLEALNAAHPDVVAVMTGPLVLMPLMESGAAPDAALSRDSLLAAGRTGPAQWRVNGP
                     QGPLTLAPFMAIGGETYRLYSRVV"
     gene            complement(1872..2255)
                     /locus_tag="VF402_10890"
     CDS             complement(1872..2255)
                     /locus_tag="VF402_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX7800849.1"
                     /translation="MSRFPHKMTAAVVSLGLLTGLAAIPDMADAASYVCKYQEKQSSK
                     TGTVVGAVAGGAIGGSLADTHNKGLGTVAGAVVGGLVGSKMGKNHGKQRCEDEHAWRT
                     RTVWEKDRHGRRHKVVYKYVDPRYN"
     gene            complement(2354..>2604)
                     /locus_tag="VF402_10895"
     CDS             complement(2354..>2604)
                     /locus_tag="VF402_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX7800850.1"
                     /translation="IRFVLHPETQASLARDRKIILLRGPGGRGWWLRHDARDVTLEEN
                     GVVFDAGQARRTTVIALRGSCRLDAATRVRWKLSPAEA"
BASE COUNT          419 a          899 c          843 g          443 t
ORIGIN      
        1 gacccgcggc caaaacccgc cgtcacgccg cgcctgcaac cggccgccta tgcgcaggtc
       61 aggctggccc acggcccgat gctgagccag cgcgcgcatc agcacgccct ctatatgggc
      121 ctcgacaacg accggttgct caagccgttc cgcgtgcgcg ccggtctgcc cgcgccgggg
      181 ccggacatgg gcggctggta cgaccagtcg gacgatttcg tcatcgatac gcacgactgg
      241 agcaagacca actggcacgg ctatattccc ggccacagct tcggccagta cctgtcgggg
      301 ctggcgcgcc tctacgcggt cacgggcgac gcggcgacca gggccaaggt cgccagtctg
      361 gtccgcgact atgccccgac catctcgccg cgcttcttcg acggctacac cctgcccgcc
      421 tacacctacg acaagatcgt catcggcctg atcgacgcct atcgctacgc cgacgtcaag
      481 gaagccgccg gggcgctgga caaggtcacc gacgccgcct tgccctatct gccggagcgc
      541 gccctgacgc gcgagcagcg ccgcaagctg ccctatgcca gcgaggcgca gatctgggac
      601 gagccctaca ccctgccgga aaacctgttc aaggcgtggc ggctgggtct gggccagcgc
      661 tattacgatc tggccgtgcg ctatcttcag gacgaggcgc tgttcgatcc gctggcgcag
      721 ggcgtcagcc cgctcaaggg caggcacgcc tacagccacg tcaacgcgct cagttccgcc
      781 atcgaagcct atctggccac gggcgacgag acatatctgc gcgcggcggc caacggtttc
      841 gccttcatcg agcgccagtc cttcgcgacc ggcggctggg ggccgaacga gaccctgatc
      901 gggccggacg atgacgacgc gctgctgacc agcctgagcc agacgcacag ttcgttcgaa
      961 acgccgtgcg gcgcctacgg ccatttcaag atcacgcgct cgctgctgcg actgaccggc
     1021 gacagccatt acggcgacag catggagcgc gtgctgtaca acaccattct gggcgcgctg
     1081 ccgaccacgc cggagggcaa gaccttctat tattcggact ataaccgcac ggccggcaag
     1141 ttctaccggc cggagctgtg gccgtgctgt tcgggaacct tcatccagtt gaccgccgat
     1201 tacggcgtca gcgcctatct gagcaacgcg cgcgccgtct atgtcaatct ctacctgccg
     1261 tccgatctgc aaaccgaaat cggcggcgtc ggcgtgcggc tgcgccagac gacggattat
     1321 ccgctggccg atacggttcg catcgacgtg cagccggaaa gaccggcgcg ctttacattg
     1381 gcgctgcgca tgcccgcctg ggcgggaccg gccacgcgcg tcacggtcaa cggcgaggcc
     1441 gttccggtcg cgccggggcg cttcctgcgg ctggagcggc tatggcgcgc gggcgatgcg
     1501 gtcgagatcc gcttcgatat ggggctgcgg ctggaggcgc tgaacgccgc gcacccggac
     1561 gtggtggcgg tgatgaccgg accgctggtc ctgatgcctc tcatggagtc cggcgccgcg
     1621 cccgacgccg ccctgagccg cgacagcctg ctggccgccg ggcggaccgg accggcgcag
     1681 tggcgcgtca acggcccgca aggaccgctg actctggcgc cgttcatggc gattggcggc
     1741 gagacctacc ggctctacag ccgggtggtt tagagcgttt tccgatctga ttgcatcaga
     1801 tcggcgctct aactttatgc ttttacgcgc tttttaatcc gaaaagtgcg tcacactttt
     1861 cggaaagcgc tctaattgta gcgcgggtcg acgtatttat agacgacttt gtggcggcgg
     1921 ccgtggcggt ccttttccca gaccgtgcgc gtgcgccagg cgtgttcgtc ctcgcagcgc
     1981 tgcttgccgt ggttcttgcc cattttcgag ccgaccagac cgccgaccac ggcgcccgcg
     2041 accgtgccga ggcccttgtt gtgcgtgtcg gccagcgagc cgccgatggc gccgcccgcg
     2101 accgcgccga ccaccgtgcc ggtcttgctg ctttgctttt cctgatactt gcacacatag
     2161 ctggcggcgt cggccatgtc cggaatggcg gccagacccg tcagcaggcc gaggctgacc
     2221 accgccgcgg tcatcttgtg agggaatcgg gacataaaat cctccttatg cagggcttcc
     2281 gtctttaaca gacgcgcagt atcggcgccg cgccataagg atgcagtcgg taaggggcgt
     2341 ggaattgtaa aggtcaggct tcggcgggcg acagcttcca gcgcacgcgc gtcgccgcgt
     2401 ccagccggca ggagccgcgc agggcgatca cggtggtccg gcgcgcctgt cccgcatcga
     2461 acaccacgcc gttttcctcc agcgtgacgt cgcgcgcgtc gtgacgcaac caccagccgc
     2521 gtccgcccgg cccgcgcagg agaatgatct tgcggtcgcg cgccagcgac gcctgcgtct
     2581 ccgggtgcag cacgaagcgg atgg
//
LOCUS       DASVLL010000664         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_422311,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000664 DASVLL010000000
VERSION     DASVLL010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422311"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1725)
                     /locus_tag="VF402_10900"
     CDS             complement(118..1725)
                     /locus_tag="VF402_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800851.1"
                     /translation="MSQMMLPNFEAPAPILIVDDLEENLLSLAAVLRQNGLEILKARS
                     GVEALEILLERDVALALLDIQMPDMDGFELAETLRGNSRTRQIPIIFLTAGGADRQRR
                     FRGYESGAVDFLEKPLEPDILRSKVAIFCELYVQRRQIAVQRDNLRAFSEQNMRLLRE
                     SRAYAEALKAADQRKDEFLATLAHELRNPLAPIRNGLQILRMAPEGPRAEEVRGMMDR
                     QLTHLVRLIDDLLDVSRVSRGKIDLRRVRMKLQDAVHAALEASKPLIEASGHTLTLHM
                     PEAPLWVDGDLTRLAQVASNLLNNAAKYTPEGGKISLRLSRDDRFAQIEVADNGLGID
                     AAMLPRVFELFAQSDQHLERAQGGLGIGLALVAKLVEMHGGSVKADSGGAGAGARFTV
                     RLPLALSEVAAPVIAEAAESAASPLHVLVVDDNVDAAQTTRWMLELNGHLPSVAHDGA
                     AALAEARRLRPDVILLDIGMPGMDGYEVCRALRAMPETRDAMIIAQTGWGQESDRQRA
                     FEAGFDHHITKPISLDALNALLVRLDG"
BASE COUNT          281 a          652 c          611 g          291 t
ORIGIN      
        1 atccgaaaag tgcgtcacac ttttcggaaa gcgctccagg cccgcgcgcc tacactgtca
       61 gccgcgccgt aagcgcaaac tagcaagccc tgcccgcttt tgtaaaatag atgtaaatca
      121 gccgtcgagc cggaccagca gcgcgttgag cgcgtcgaga ctgatcggct tggtgatatg
      181 gtggtcgaag cccgcctcga acgccctttg ccggtcgctt tcctgccccc agccggtctg
      241 ggcgatgatc atggcgtcgc gcgtctccgg catggcgcgc aaggcgcggc aaacctcgta
      301 gccgtccatg cccggcatgc cgatatcgag caggatcacg tcgggccgca accgccgcgc
      361 ctcggccagc gccgccgcgc cgtcatgcgc cacgctgggc agatggccgt tcagctccag
      421 catccagcgc gtcgtctgcg ccgcgtcgac attgtcgtcg accaccagca cgtgcagcgg
      481 cgacgccgcg ctttcggcgg cctcggcgat cacgggcgcc gcgacctcgc tgagcgccag
      541 cggcaggcgg accgtgaagc gcgcgcccgc gccggcgccg ccggaatcgg ccttcaccga
      601 gccgccgtgc atctcgacca gcttggcgac cagggccagc ccgatgccca gaccgccctg
      661 cgcgcgctcc agatgctgat ccgactgggc gaacagctcg aacacgcgcg gcagcatggc
      721 cgcgtcgatg cccagcccgt tgtcggcgac ctcgatctgc gcgaagcggt cgtcgcgcga
      781 cagtctgagc gagattttgc cgccctccgg cgtatatttg gcggcgttgt tcaaaaggtt
      841 cgacgccacc tgcgccagcc gcgtcaggtc gccgtcgacc cagagcggcg cctcgggcat
      901 gtggagcgtc agcgtgtgac cgctggcctc gatcagcggc ttcgacgcct ccagcgcggc
      961 gtggaccgcg tcctgcagct tcatgcgcac gcggcgcagg tcgatcttgc cccggctgac
     1021 gcgcgacacg tcgagcaggt cgtcgatcag ccgcaccaga tgggtcagtt gccggtccat
     1081 catgccgcgc acctcctcgg cgcgcggccc ttcgggcgcc atgcgcagga tttgcaggcc
     1141 gttgcggatc ggcgccagcg ggttgcgcaa ttcgtgcgcc agcgtggcga ggaactcgtc
     1201 cttgcgctgg tcggcggcct tcagcgcctc ggcataggcg cgcgattcgc gcagcagccg
     1261 catgttctgc tcggaaaagg cgcgcagatt gtcgcgctgc accgcgatct ggcgacgctg
     1321 cacgtacagt tcgcagaaga tggccacctt ggagcgcagg atgtccggct ccagcggctt
     1381 ttcgaggaag tcgaccgcgc cggattcata gccgcggaaa cgcctttgcc ggtcggcgcc
     1441 gcccgccgtc aggaagatga tcggaatctg gcgcgtgcgc gaattgccgc gcagggtttc
     1501 ggccagctcg aacccgtcca tgtcgggcat ctggatgtcg agcagggcca aagcgacgtc
     1561 gcgctccagc aggatctcca gcgcctcgac cccggagcgc gccttgagga tctccagccc
     1621 gttctgacgc agcaccgccg ccagcgacag caggttctcc tccagatcgt cgacgatcag
     1681 gatcggcgcg ggggcctcga aattcggcag catcatctgg ctcatgcgcg gacctcgcgc
     1741 gacagttcgg cggcgatggc gtccagcggc agcacctgcg cgcccggcgc ggcgtccagc
     1801 gccgcctgcg gcatggccgg ggcgtaggcg cccgc
//
LOCUS       DASVLL010000665         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_207221,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000665 DASVLL010000000
VERSION     DASVLL010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207221"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF402_10905"
     CDS             <1..213
                     /locus_tag="VF402_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7800852.1"
                     /translation="MRAVLETLGVSDVVAKSLGSSNPYNMVRATFEALKVQSSPRQVA
                     SKRGLKVGDLLGRRNDGASSPESLEG"
     gene            217..402
                     /gene="rpmD"
                     /locus_tag="VF402_10910"
     CDS             217..402
                     /gene="rpmD"
                     /locus_tag="VF402_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX7800853.1"
                     /translation="MAEAKTITVKQTGSPIRRTKDQRATLAGLGLNKLGRVSTLQDTP
                     SVRGMIAKVAHMVEIVG"
     gene            591..1070
                     /gene="rplO"
                     /locus_tag="VF402_10915"
     CDS             591..1070
                     /gene="rplO"
                     /locus_tag="VF402_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX7800854.1"
                     /translation="MTKLNEIRDNEGSHKGRMRVGRGPGSGKGKTSGRGVKGQKSRTG
                     VSLLGFEGGQMPLYMRMPKRGFNNPFALKFAEVNLWRLQAAVDAGKLDASKPIDAAAL
                     KAAGVIRRELDGVRLLGEGELKAKLDLSVYSATAGAAKAVEAAGGKLTQARPVKTEA"
     gene            1228..>1818
                     /locus_tag="VF402_10920"
     CDS             1228..>1818
                     /locus_tag="VF402_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7800855.1"
                     /translation="MASAAEQLAANLNFGAFAKATDLHKRLLFTLGALIVYRLGTYIP
                     LPGIDMAAFQHAFNDQSKGIFGMMNMFSGGAVQRMAVFALNLMPYISASIIIQLMGSV
                     YAPWEKLRKEGGEAGRKQLNQYTRYLTVFLGLVQSFGIAEAMQAGGMAIDPGPFFIIT
                     AMVTLTGGTLFLMWLGEQVTARGIGNGSSIIIFAGIV"
BASE COUNT          322 a          580 c          588 g          328 t
ORIGIN      
        1 atgcgcgccg ttctcgaaac cctgggcgtt tcggacgtcg tggccaagtc gctcggctcc
       61 tcgaacccct acaacatggt gcgcgccacc tttgaagccc tgaaggtgca gtcctcgccc
      121 cgtcaggtcg cttccaagcg cggcctcaag gtcggcgacc tgctgggccg ccgcaacgat
      181 ggcgcctcgt cgccggaatc gctggagggc taagtcatgg ctgaagcgaa aaccatcacc
      241 gtgaagcaga ccggttcgcc gatccgccgc accaaggacc agcgcgccac gctggcgggc
      301 ttgggcctga acaagctggg ccgcgtgtcc accttgcagg acaccccgtc ggtgcgcggc
      361 atgatcgcca aggtcgccca catggtcgag atcgtcggct aagcccggcc ttttcgtgaa
      421 gatcgcagcg cccgcaggtt cttcctgcgg gcgtttcgct atttatgagt cccgattctt
      481 tagagagtcg ggttgtgcgg cctgcctttt gtttgtaagg aggccgttca aaagtgaatc
      541 gagccgagtg gagttgtgga aactccgcgt attcttaagg agaaggcgat atgacgaagc
      601 tgaacgagat ccgcgacaac gaaggctccc acaagggccg catgcgcgtc ggccgcggcc
      661 ccggttcggg caagggcaag acctccggcc gcggcgtcaa gggccagaag tcccgcaccg
      721 gcgtgagcct gctgggcttc gaaggcggcc agatgccgct gtacatgcgc atgcccaagc
      781 gcggcttcaa caacccgttc gcgctgaagt tcgccgaagt caacctgtgg cgtctgcaag
      841 ccgccgtcga cgcgggcaag ctggacgctt cgaagccgat cgacgccgcc gcgctgaagg
      901 ccgccggcgt gatccgccgc gagctggacg gcgtgcgcct gctgggcgaa ggcgagctga
      961 aggccaagct ggacctgagc gtctattccg cgaccgcggg cgccgccaag gcggtggaag
     1021 ccgcgggcgg caagctgacg caagcccgtc cggtcaagac cgaagcgtaa ttgccgatcc
     1081 gggacggtca tcgcggccat cccggatttg acctttcggt ttatgtatcc catatggttg
     1141 atgagcctgc ggcgacacgc ccggttccat ctcccgccgg gacaacatag ataagggcgt
     1201 aaagccccgc gctttcgagg atcgtcaatg gcttcagccg ccgaacaact tgccgccaat
     1261 ctgaatttcg gcgccttcgc caaggcgacg gacctgcaca agcgcctgct ctttacgctg
     1321 ggggcgctga tcgtctatcg cctgggcacc tatattccgc tgcccggcat cgacatggcg
     1381 gcgttccagc acgccttcaa cgatcagtcg aagggcatct tcggcatgat gaacatgttc
     1441 tccggcggcg ccgtgcagcg catggcggtg ttcgcgctga acctgatgcc gtacatctcg
     1501 gcctcgatca tcatccagtt gatgggttcg gtctatgcgc cgtgggaaaa gctgcgcaag
     1561 gaaggcggcg aggcggggcg caagcagctc aaccagtaca cccgctacct gaccgtgttc
     1621 ctggggctgg tgcagtcgtt cggcatcgcc gaggcgatgc aggcgggcgg catggcgatc
     1681 gatccggggc cgttcttcat catcaccgcc atggtcaccc tgaccggcgg caccctgttc
     1741 ctgatgtggc tgggcgagca ggtgaccgcg cgcggcatcg gcaacggctc gtcgatcatc
     1801 atcttcgcgg gcatcgtg
//
LOCUS       DASVLL010000666         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_531519,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000666 DASVLL010000000
VERSION     DASVLL010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531519"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VF402_10925"
     CDS             complement(<1..350)
                     /locus_tag="VF402_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800856.1"
                     /translation="MKSVWLAAFAALIAAPAFAQVQPTYIPAPPPLAHDRLPAVVDAA
                     RRAAIRRIEPDKIILVGDSTTQVGSGWGGAFCDNHVTSYLACVNLARGGRGTFDYRAE
                     GSWDIALGEMKVPG"
     gene            complement(383..1351)
                     /locus_tag="VF402_10930"
     CDS             complement(383..1351)
                     /locus_tag="VF402_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEX7800857.1"
                     /translation="MLFAIAALTAPAFAQARTVTISADGKGAYPTLQAAIDALHDTGG
                     EIDLQPGVYREKATVSGDDLHLKGLGAGPEAVVIVWGDSAASTGSTFKSSTLLASGDG
                     FRADNLTIQNDYAQHTDVGSQAVALSLTGDKAVLTHVRLLGAQDTLYVNKGPQGRMSR
                     DYFADCYIEGHVDFIFGNAKAYFHGCEIHGVAHAEVMYTAQSKNAPDEDSAFVFEDCT
                     FTAGPGAQKVSLGRAWRAYATVILLNARIDTPLIKGGWREWRPGQTETLKTATYAEYK
                     STGPGADPAGREPYSHQLTDAQAAAWRLNAFFKGDVGWLPPEAQKQ"
BASE COUNT          232 a          495 c          520 g          274 t
ORIGIN      
        1 tagcccggaa ccttcatctc gcccagcgcg atgtcccatg agccttcggc gcggtaatcg
       61 aaggtgccgc gcccgccgcg cgccagattg acgcaggcga ggtaggaggt gacgtggttg
      121 tcgcaaaagg cgccgcccca gccgctgccg acctgcgtgg tggaatcgcc gacgaggatg
      181 atcttgtccg gttcgatgcg ccggatcgcg gcgcgcctgg ccgcgtccac gaccgccgga
      241 agccggtcat gcgccagcgg cggcggcgcg gggatgtagg tcggctggac ctgagcaaag
      301 gcgggcgcgg cgatcagggc ggcgaaggcg gcgagccaga cggatttcat gagcgtgtcc
      361 tttttcctgc ggcgttcagg ctttattgtt tttgcgcttc gggcggcagc cagccgacat
      421 cgcccttgaa aaaggcgttc agccgccatg ccgcggcctg cgcatcggtg agctgatggc
      481 tgtacggctc gcggcccgcg gggtcggcgc cggggccggt cgatttatat tcggcgtagg
      541 tcgcggtttt cagcgtctcg gtctggcccg ggcgccattc gcgccagccg cccttgatca
      601 gcggcgtatc gatcctggcg ttcagcagga tcaccgtcgc gtaagcgcgc cacgcgcggc
      661 cgagactgac cttctgcgcg cccggtcccg ccgtgaacgt gcaatcctcg aacacgaagg
      721 cgctgtcctc gtccggcgcg ttcttgctct gcgccgtgta catgacctcg gcatgggcga
      781 cgccgtggat ttcgcagccg tggaaatagg ccttggcgtt gccgaagatg aaatcgacat
      841 ggccctcgat atagcagtcc gcgaaatagt cgcggctcat gcggccctgc ggccccttgt
      901 tgacgtaaag cgtgtcctgc gcgcccagaa gccgcacatg ggtcagcacg gccttgtcgc
      961 cggtcagcga caaagccacc gcctgcgagc cgacatcggt gtgttgggca tagtcgttct
     1021 ggatggtcag gttgtcggcg cggaaaccgt cgccgctggc cagcagggtg gaggatttga
     1081 aagtcgagcc ggtcgaggcc gcgctgtcgc cccagacgat cacgaccgcc tccggtccgg
     1141 cgccgaggcc ctttaagtgc aggtcatcgc cgctgaccgt cgccttctcg cgatagacgc
     1201 ccggttgcag gtcgatctcg ccaccggtgt catgcaaggc gtcgatcgcg gcctgcaagg
     1261 tgggataggc ccccttcccg tccgccgaga tcgtcacggt ccgcgcctgg gcgaaggcgg
     1321 gcgccgtcag ggccgcgatc gcgaaaagca tggctttcat cattacctcc tcaggcgcgg
     1381 aacacgaccg tcttgccggc ggtcaggtcc agcggggtta tcttatcgtc ataacgcacg
     1441 cgcacgctgc gatccgtcgc gcaggtcagg gccagagttt gcagccggcc gtcccgccat
     1501 gcaaggtccg cgtcgatccg g
//
LOCUS       DASVLL010000667         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_531539,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000667 DASVLL010000000
VERSION     DASVLL010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531539"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(388..924)
                     /gene="ppa"
                     /locus_tag="VF402_10935"
     CDS             complement(388..924)
                     /gene="ppa"
                     /locus_tag="VF402_10935"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX7800858.1"
                     /translation="MNIDKISVGPNAPWDLHAVIEIPQGGMPVKYEMDKDSGALFVDR
                     FLYTSMFYPGNYGFIPHTLADDGDPCDVMVVGPTPVYPGVVIRCRPIGALVMEDEAGR
                     DEKILAVPVDKLHPYYTNVASYRQMPQILIEQITHFFAHYKDLEKGKSTKVIGWVEPE
                     AAAQLIREGQERLAQKIG"
     gene            1111..1902
                     /locus_tag="VF402_10940"
     CDS             1111..1902
                     /locus_tag="VF402_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HEX7800859.1"
                     /translation="MTALQALSATLMRSPLDLLAFAIFVVCWLGYESIVRVLSKKAGL
                     IVKDLSVVRQTWMKEMVIRSFKLFDSNLMGHAVNSATNFSSANLLLIAAIAGVMFSGQ
                     FPVQSARALGIDVSSPHILQLRLALVLICLTRGLLNFIWSIRQMNYCAAAIGSLPDNM
                     DAPTARDFGEAMGDIIEPAMSNFSQGVRSYYFSLAAGAWLFGPLALIAASFGAVALLG
                     WRQSRSRAARGLRRLRELLEAHPYPTPTRPIQTGGEAGGDNALQN"
BASE COUNT          361 a          649 c          607 g          416 t
ORIGIN      
        1 aaaatttcac gaaagcgtga taataagtaa aaaatttcat gttagcgcta ccgcgcacga
       61 ctttattcta agaaaaacac aaatttccta caggcgcgaa caaccgcgcc agaaatccag
      121 ccgcgctttc aggctgcccc ctccctaccc ctctcccctc cacgagggga gaggacgaga
      181 gccgagtcgt caggcgaagg cgatcgggtg aggggctgcc agccgtttgc gctcaaccct
      241 ccgccagccc ctcacccgag gatcggcggc ttcgccttgc tcctcgtcct ctccccattg
      301 tcatggggag agggggtagg cggcgctact tcgatacaaa aaaggccggc gtcgccgccg
      361 gcctttttgc aaccaatgaa acggcgatca gccgatcttc tgcgccagac gctcctggcc
      421 ttcgcggatc agttgcgcgg cggcttccgg ctcgacccag ccgatgacct tggtgctctt
      481 gcccttttcc agatccttgt agtgcgcgaa gaagtgcgtg atctgctcga tcaggatctg
      541 cggcatctga cggtagctgg cgacattggt gtaatagggg tgcagcttgt ccaccggcac
      601 ggccaggatc ttctcgtcgc ggcccgcctc gtcctccatg accagcgcgc cgatcgggcg
      661 gcagcggatc accacgcccg gatagaccgg cgtcgggccg accaccatca cgtcgcacgg
      721 atcgccgtcg tcggccaggg tgtgcgggat gaagccgtaa ttgcccggat agaacattga
      781 ggtgtacagg aagcggtcga cgaacagggc gccggaatcc ttgtccatct cgtacttcac
      841 gggcatgccg ccctgcggaa tttcgatgac cgcgtggagg tcccacggcg cgttgggacc
      901 gacggagatt ttatcgatat tcatgatctt tttcgacttt tgaacagaag ttggcggctg
      961 tcataagggc ttggcctcct tcgcacaagc gagaatgaag ccgggcgatc aatctttatg
     1021 acgatttgcc cgccgccctg tcgcgaaagc gcgcataagc ggttagaatc gccggcgatt
     1081 cccgttcccg cgcgctcctt ccgaggctcc atgaccgccc tgcaagccct gtccgccacc
     1141 ctgatgcgca gtccgctgga cctgctggcc ttcgccatct tcgtcgtctg ctggctgggc
     1201 tacgaatcga tcgtgcgcgt cctgtcgaag aaggcgggcc tgatcgtcaa ggatctgagc
     1261 gtggtgcgcc agacctggat gaaggagatg gtgatccgca gtttcaagct gttcgactcc
     1321 aacctgatgg gccacgcggt caattcggcc accaacttct cgtcggccaa cctgctgctg
     1381 atcgccgcca tcgccggggt gatgttcagc ggccagttcc cggtgcagtc ggcgcgggcg
     1441 ctgggcatcg acgtgtcctc gccgcacatc ctgcaattgc ggctggccct ggtgctgatc
     1501 tgcctgacgc gcggcctttt gaacttcatc tggtcgatcc gccagatgaa ctattgcgcc
     1561 gccgccatcg gctcgctgcc cgacaatatg gacgcgccga ccgcgcgcga cttcggcgag
     1621 gcgatgggcg acatcatcga accggccatg tcgaactttt cgcagggcgt gcgcagctat
     1681 tacttctcgc tggccgccgg ggcgtggctg ttcgggccgc tggccctgat cgcggccagc
     1741 ttcggcgccg tcgccctgct gggctggcgc cagtcgcgct cgcgcgccgc caggggcctg
     1801 cgccgcctgc gcgagctttt ggaggcgcat ccctatccga cgccgacgcg cccgatccag
     1861 accggcggcg aggcgggcgg cgataatgcg ctgcaaaatt gagaacggcg gttcttgatc
     1921 gtacttttca cgcccaaaac cttaaattca gcgcaattgt aacgatacgt gatcgcaact
     1981 gcgacaattt gcgcttgagt tattttgcgc cgcagcgtaa tttcaacctg ttc
//
LOCUS       DASVLL010000668         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_368740,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000668 DASVLL010000000
VERSION     DASVLL010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368740"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF402_10945"
     CDS             <1..369
                     /locus_tag="VF402_10945"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7800860.1"
                     /translation="SRFRLYASAAYLEATPPEAWEFVAYDETLDAAPQQARLLWLLKG
                     RAIGLRASTLDMQLAFLLADGGVGMLPDFVAEGRDGHVAADPDEPPLTREIWLVVHTD
                     MKDAPAIRAVMDGFGHQART"
     gene            complement(420..>2455)
                     /locus_tag="VF402_10950"
     CDS             complement(420..>2455)
                     /locus_tag="VF402_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800861.1"
                     /translation="DLVIGGDKSGMQSSYYDRTSYFGTLEFKPNDRFHAVFDAFHSDF
                     TELQTVRRMEYGTIWGTGVLQPGYTVSDGRIASGEFDGVTTIIENYNYLRHAKIDNFG
                     LNLNYDLNDKWTLNADISDSKVHRTDLRLESTGGTGPAGSVVKNDVTFTTDADGVTRF
                     HSDFDFSDFDKMFLTDPGGWGGPDTRAGYVGNPTVNDEIKAVRLTAARTLEGPFSRIS
                     FGVNYADRAKSMYQWQGMLINRDGSAMSVPDAYRNGVIDTSFLGNPGGMISYDALGLY
                     NSGFWSVTDARVDPVALAGDRIFDITQSWDMDEKLTTLYVKADIDTHLFGVPLTGNIG
                     LQSQTADQSVIEGFTPGPDPVTNALPVTTVKDGDKYTNVLPSMNLNFALADDMVLRVA
                     AAKTLSRPPMDYMAGGVSYTVAADGQPPYGASDNPYYWSASGGNAHLRPWQATAYDMS
                     FEKYFGRKGYVSAAVYYKTLDSYIYYKQLPYDFSGFPLPAASGAATYANAGADRKGYI
                     GAQANGSGGYVKGLELTASVPGEVVSPWLDGFGVILSAAFNDSQIKPDGVNAIPLPGL
                     SPSVINGTVYYEKHGLSLRVSDRFRGAWLGQVPNFDSSLGSQWIAKEQVVDAQIGYSF
                     DSGPLKGFSINLAGYNLTNQPFVYYAGKGQTQNILKYESYGADYMLSVGYKFF"
BASE COUNT          418 a          763 c          815 g          459 t
ORIGIN      
        1 agccggttcc ggctttacgc cagcgcggcc tatctcgaag cgacgccgcc cgaagcctgg
       61 gaattcgtcg cttatgacga aaccctggac gccgcgcccc agcaggccag actgctgtgg
      121 ctgctgaaag gccgcgcgat cgggttgcgc gccagcacgc tcgacatgca actggccttc
      181 ctgctggcgg atggcggcgt cggcatgctg cccgacttcg tcgcggaagg ccgggacggg
      241 cacgtcgccg ccgatcccga cgaaccgccg ctgacgcggg aaatctggct ggtcgtacac
      301 acggatatga aggatgcgcc cgcgatccgc gcggtcatgg atggtttcgg tcatcaggcc
      361 cgcacatgaa aaacgcccgc tggggcgacc ggcgggcgtt tccatcgtcg tcatacgcct
      421 tagaagaact tgtagccgac gctgagcatg taatcggcgc cgtaggactc gtatttcagg
      481 atgttctggg tctggccctt gcccgcgtaa tagacgaagg gctggttggt caggttgtaa
      541 ccggccagat tgatcgagaa gcccttgagc ggcccggagt cgaagctgta gccgatctgg
      601 gcgtcgacca cctgctcttt ggcgatccac tgcgaaccga gcgaggagtc gaagttcggc
      661 acctgaccca gccacgcgcc gcggaaacgg tctgaaacgc gcagcgacag gccgtgcttt
      721 tcataataga ccgtgccgtt gatcacgctg ggcgacaggc cgggcagcgg gatcgcgttc
      781 accccgtccg gcttgatctg cgagtcgttg aaggcggcgc tcaggatcac gccgaagccg
      841 tccagccacg gcgagacgac ctcgcccgga accgaggcgg tcagctccag acccttgacg
      901 tagccgccgc tgccgttggc ctgagcgccg atatagccct tgcggtcggc gccggcgttg
      961 gcgtaggtgg ccgcgccgct tgccgccggc agcgggaagc cggagaagtc gtagggcaac
     1021 tgcttgtaat agatgtagct gtccagcgtc ttgtaataga ccgccgccga gacatagccc
     1081 ttgcgcccga aatacttctc gaacgacata tcgtaggcgg tggcctgcca cggacgcagg
     1141 tgcgcgttgc cgccgctggc gctccagtaa tagggattgt cgctggcgcc gtagggcggc
     1201 tggccgtcgg cggcgaccgt atagctgacg ccgcccgcca tatagtccat cggcggacgc
     1261 gacagggtct tggccgccgc gacgcgcagg accatgtcgt cggccagcgc gaagttgagg
     1321 ttcatgctgg gcaggacgtt ggtgtatttg tcgccgtcct tcaccgtcgt caccggcaag
     1381 gcgttggtga ccgggtccgg gccgggggtg aagccctcga tcacgctctg gtcggcggtc
     1441 tgggattgca ggccgatatt gccggtcagc ggcacgccga acaggtgcgt gtcgatatcc
     1501 gccttgacgt agagcgtggt cagcttttcg tccatgtccc acgactgggt gatgtcgaag
     1561 atgcggtcgc ccgccagcgc cacgggatcg acgcgcgcgt cggtcacgct ccagaagccg
     1621 ctgttgtaga ggcccagcgc gtcataggag atcatgccgc cgggattgcc gaggaagctg
     1681 gtgtcgatca cgccgttgcg ataggcgtcg ggcacgctca tcgccgaccc gtcccggttg
     1741 atcagcatgc cctgccattg atacatgctc ttggcgcggt cggcgtagtt gacgccgaag
     1801 ctgatccggc tgaacgggcc ttcgagcgta cgcgccgccg tcagccgcac agccttgatc
     1861 tcgtcgttca cggtcggatt gccgacatag cccgcgcgcg tgtccgggcc gccccacccg
     1921 cccggatcgg tcaggaacat cttgtcgaag tcgctgaagt cgaaatcgga atggaagcgc
     1981 gtgacgccgt ccgcgtcggt cgtgaaggtc acgtcgttct tgaccaccga ccccgccggc
     2041 cccgtgccgc cggtcgattc cagccgcagg tcggtgcggt ggaccttgga atcgctgatg
     2101 tcggcgttga gcgtccactt gtcgttcagg tcgtaattca ggttgaggcc gaaattgtcg
     2161 atcttggcgt gacgcagata gttatagttt tcgataatcg tcgtaacgcc gtcgaactcg
     2221 cccgacgcga tgcgtccgtc cgagaccgta tagcccggtt gcagcacgcc cgtgccccag
     2281 atcgtgccgt attccatgcg ccgcacggtc tgcaactcgg tgaagtcgga atggaaggcg
     2341 tcgaacaccg catggaagcg gtcgttcggc ttgaactcca gcgtgccgaa ataggaggtg
     2401 cggtcgtaat aggaggactg catcccgctc ttgtcgccgc cgatgaccag atcgt
//
LOCUS       DASVLL010000669         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_477252,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000669 DASVLL010000000
VERSION     DASVLL010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477252"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VF402_10955"
     CDS             <1..1233
                     /locus_tag="VF402_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800862.1"
                     /translation="SVQKNNEDVRSVRVRPDSKGIRARLENGTAYFTLTKPENLSVEF
                     DGDHLHNLHLFTHAIRKDMPAHPAVTSYDIGAAPNPDLKGPVVIFGPGVYTPPDGPGG
                     SYRIGSNTTVYVDGSALIRGTFVIENAENVRIVSDGLFEGQKDLLWVRNSRHVEIDGP
                     IVFNPVHGSMRCAQSQDVVFRNIRIIGGGQWSDGIGNFACQNVTIADSFIRTSDDSVT
                     IYNHRWDIYGDSRDITLRDSTLWADIAHAVFIGLHGNTPSAGHPGPEVIERVRVSNVD
                     ILDEDEDEPEYEGALGITAGDDNLIRDVTFENIRVERIEEGRLFNFHVGFNAKYNTSP
                     GRGIENVTLRNVSYSGLGAAGPSTIFGYDAGRGVRHVVLDNVRVGGRRIGPQDKDLLQ
                     VGDFASDVVFADPSEKSQ"
     gene            1243..2046
                     /locus_tag="VF402_10960"
     CDS             1243..2046
                     /locus_tag="VF402_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase"
                     /protein_id="HEX7800863.1"
                     /translation="MQDPDQIVALSDGPANAFSDVWPSRRLREVGRAELWSFRASRPR
                     ASVLVCAGGGYVELVVDREGIDVAIWLAGQGYDAHVLVHRLPGAPGPDGRAYPSDIAL
                     GDAMAALRHIRRDLPLFLMGLSSGGHLAGAVACQAGVDPAGLLIGYAPLNANHRAYKA
                     PAGKPDYAPVEKQDFYDAWPIGLTAEPHGLPACPVFLAYALHDNPVPIEHALNFVRSG
                     RDRGLDVDAHIFGRAPHGFALRDADGTHGVWPQLAQQWMSEKLTRFGRP"
     gene            complement(2062..>2365)
                     /locus_tag="VF402_10965"
     CDS             complement(2062..>2365)
                     /locus_tag="VF402_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7800864.1"
                     /translation="HKTAEDVLSLSRQGRADAVQAVERLKSRLARALASVCNLCDPET
                     IVLGGGLSNAAEIYDGLEDRVREHVFSDAWRADIRRARWGDSSGVRGAARLWRDGE"
BASE COUNT          386 a          784 c          791 g          404 t
ORIGIN      
        1 tcggtgcaga agaacaatga agatgtccgc tcggtccggg tgcggcccga ctccaagggg
       61 atcagggcgc ggctcgagaa cgggaccgcc tatttcacgc tgacgaaacc ggaaaatctg
      121 agcgtggagt tcgacggcga tcacctgcac aatctgcacc tgttcaccca tgccatccgc
      181 aaggacatgc ccgcgcatcc ggccgtgacc agctatgaca tcggcgcggc gccgaatccc
      241 gatctgaaag ggccggtcgt gatcttcggc cccggcgtct atacgccgcc ggacggtccg
      301 ggcggcagct acaggatcgg ctcgaacacc acggtctatg tcgacggctc ggcgctcatc
      361 cgcggtacgt tcgtgatcga gaacgccgag aatgtccgca ttgtcagcga cggcctgttc
      421 gaggggcaga aggatctgct gtgggtgcgc aattcccggc atgtcgagat cgacgggccg
      481 atcgtcttca atcccgtgca cggctcgatg cgctgcgcgc agtcgcagga cgtggttttc
      541 cgcaatatcc gcatcatcgg cggcggccag tggtcggacg gcatcggcaa tttcgcctgt
      601 cagaacgtga cgatcgccga ttccttcatc cgcacctcgg acgacagcgt caccatctat
      661 aaccaccgct gggacattta cggcgactcg cgcgacatca cgctgaggga ttcgaccctg
      721 tgggccgata tcgcgcacgc ggtcttcatc ggcctgcacg gcaatacgcc gtcggccggg
      781 catcccggcc ctgaggtgat cgagcgggtg cgcgtcagca atgtcgacat tctcgacgaa
      841 gacgaggacg agcccgaata tgagggcgcg ctggggatca cggcgggcga tgacaacctc
      901 atccgcgacg tgaccttcga gaacatccgc gtcgagcgca tcgaggaagg ccgcctgttc
      961 aacttccatg tcggcttcaa cgccaagtac aacacctcgc cggggcgggg catcgagaac
     1021 gtcaccctgc gcaatgtcag ctattccggc ctgggcgccg ccgggccgtc cacgattttc
     1081 ggctatgacg ccggacgcgg cgtgcggcat gtcgttctgg acaatgtgcg cgtcggcggg
     1141 cggcgcatcg gcccacagga caaggatctg ttgcaggtcg gcgatttcgc ttccgacgtc
     1201 gtcttcgccg atccctcaga aaaaagccaa taaagacacg acatgcaaga ccccgatcag
     1261 atcgtcgcgc tcagcgacgg acctgcaaac gccttttccg acgtctggcc cagccgccgc
     1321 ctgcgcgagg tcgggcgggc ggaattgtgg agcttccggg ccagccgccc gcgcgcttcg
     1381 gttctggtgt gcgcgggcgg cggctatgtc gagctggtcg tcgaccggga ggggatcgat
     1441 gtcgcgatct ggctcgccgg gcagggatac gacgcgcatg ttctggtcca tcgcctgccc
     1501 ggcgcgccgg ggccggacgg gcgcgcctat ccgtccgata tcgcgctcgg cgacgcgatg
     1561 gccgcgctgc gccacatccg gcgggatttg ccgctgttcc tgatggggct gtcttcgggc
     1621 gggcatctgg cgggggcggt ggcctgtcag gccggggtcg atcccgccgg tttgctgatc
     1681 ggctacgcgc cgctcaacgc caatcaccgc gcctacaagg ctccggcggg caagcccgat
     1741 tacgcgccgg tcgagaagca ggatttttac gacgcttggc ccatcggcct gaccgcggag
     1801 ccgcacggcc tgcccgcctg tccggttttc ctggcctacg cgctgcatga caatcccgtg
     1861 ccgatagagc acgccctgaa cttcgtgcgc agcggccgcg atcgcgggct ggacgtcgac
     1921 gcgcatattt tcggacgggc gccgcacggc ttcgcgctgc gggacgcgga cggcacgcac
     1981 ggggtctggc cgcaattggc gcagcagtgg atgtcggaga agctcacacg cttcgggcgg
     2041 ccgtgaaacg cggcggcggt tttattcgcc gtcgcgccag agccgcgccg cgccgcgcac
     2101 gccggaggaa tcgccccagc gggcgcggcg gatatcggcg cgccaggcgt cgctgaagac
     2161 gtgttcgcgc accctatcct ccagtccgtc atagatttcc gccgcgttgc tcaggccgcc
     2221 gcccagcacg atggtttcgg gatcgcagag gttgcacacg ctggccagcg cgcgcgccag
     2281 ccggctcttg agccgttcga ccgcctgcac ggcgtccgcg cggccctgac gcgacaggct
     2341 gaggacgtcc tcggcggtct tgtgc
//
LOCUS       DASVLL010000670         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_477263,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000670 DASVLL010000000
VERSION     DASVLL010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477263"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VF402_10970"
     CDS             <1..886
                     /locus_tag="VF402_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX7800865.1"
                     /translation="AQVRDGFWWRAALPMWDSVVRAGGRSAVEFWPGSEAEVDGLRPT
                     YYNPYDGKVSNAARVDQVLKWLDLPASKRPQALLLYFDEVDHAGHSYGPDSPEVDAAI
                     AHVDAAIGRLRAGLKARGIDPNLIVVADHGMAGIALDRTYFLEDYLPKGSYDAVVQGP
                     EAEIDPAPGHAADLEKLTHMSFPHMRCWDKADIPPSYHYGRNPRVPAVLCLSDPGWMV
                     VTTHARGVWGNRGAHGYDPAAREMAALFIAHGPGVRAGVLLPSFDNVSVYDLEMHFLG
                     LTPEPNDGDLSPFAAALN"
     gene            997..1290
                     /locus_tag="VF402_10975"
     CDS             997..1290
                     /locus_tag="VF402_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800866.1"
                     /translation="MRDFLLLTHDDATEPATGEMWGAYFARLRTMGVFDGGSAIGRGE
                     AFRKTGAPDAVSARLSGYIRVRAPSLSAARELLDGHPVYEAGGTVEIRELPKS"
     gene            1328..>2022
                     /locus_tag="VF402_10980"
     CDS             1328..>2022
                     /locus_tag="VF402_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800867.1"
                     /translation="MSDKIRSARFGAAAPFILVTVFLDMISIGIIIPVLPKLILSFLH
                     GGISAAGLWTGVFGAVWGLAQFLFSPVQGGASDAFGRRPVVLASNLGLSLDYLLMALA
                     PNLWWLLVGRIVSGMTSASISTAYAYMSDVTAPEKRAGVFGLMGAAFGVGFILGPGIG
                     GVLGGIDPRLPFYAASALSMANFAYGLFVLPESLPKAKRKAFSLHAANPLGAVKFLAR
                     SAQVLRLSLMYLL"
BASE COUNT          304 a          642 c          702 g          374 t
ORIGIN      
        1 ggcgcaggtg cgggacggct tctggtggcg ggcggcgttg ccgatgtggg attcggtggt
       61 cagggcgggc ggacgctcgg cggtcgagtt ctggcccggc tccgaagccg aggtcgacgg
      121 cctgcgcccg acctattaca acccctatga cggcaaggtc agcaacgcgg cgcgcgtcga
      181 tcaggtgctg aaatggctgg acctgcctgc gtcgaaacgc ccgcaagccc tgctgctcta
      241 tttcgacgag gtcgatcacg cgggccacag ctacggcccg gactcgccgg aggtcgatgc
      301 ggccatcgcg cacgtcgacg ccgccatcgg acggctgcgc gcgggcctga aggcgcgcgg
      361 gatcgatccg aacctgatcg tggtcgccga tcacggcatg gcgggcattg cgctcgaccg
      421 gacctatttc ctcgaggatt atctccctaa aggcagttac gacgccgtcg tgcaaggccc
      481 cgaagccgag atcgatccgg cgccgggtca cgccgccgac ctcgaaaagc tgacccatat
      541 gtctttcccg cacatgcgat gctgggacaa ggcggacatt ccgccgtcat accattacgg
      601 ccgtaatccg cgcgttcccg ccgtcctgtg cctgagcgat ccgggctgga tggtggtgac
      661 cacgcacgcc aggggcgtct ggggcaatcg cggcgcgcac ggctatgatc cggccgcgcg
      721 ggagatggcg gcgctgttca tcgcgcacgg gccgggcgtc agggcgggcg tcctgctgcc
      781 gtcttttgac aatgtgagcg tttacgacct cgaaatgcat tttctcggcc tgacgccgga
      841 gccgaacgac ggcgatctca gcccgttcgc ggcggcgctg aactagagcg ttttccgatc
      901 tgattgcatc agatcggcgc tctgacttta tgctgttacg cgctttttaa tccgaaaagt
      961 gcgtcacact tttcggaaag cgctttagga gcttcgatgc gcgatttcct gctgctgacg
     1021 cacgacgacg ccacggagcc ggcgacgggc gagatgtggg gcgcctattt cgcgcggctg
     1081 cggacgatgg gcgtgttcga cggcggcagc gccatcggtc gcggcgaggc gttccgcaag
     1141 accggcgcgc cggacgcggt cagcgcgcgc ctgagcggct atatccgcgt gcgcgccccc
     1201 agcctgagcg cggcgcgcga actgctggac ggccatccgg tctatgaggc gggcggcacg
     1261 gtcgagattc gcgaattgcc gaaaagctga cggacggcta ttcaggataa aaatcacagg
     1321 gaagcgcatg tccgataaaa tcagatcggc cagattcggg gccgccgcgc cgttcattct
     1381 ggtgacggtg tttctcgaca tgatctcgat cgggatcatc attccggttt tgcccaagct
     1441 gatcctgagc ttcctgcacg gcggcatatc ggcggcgggg ctgtggaccg gcgtgttcgg
     1501 cgcggtgtgg gggctggcgc agttcctgtt ctcgccggtg cagggcggcg cgtccgacgc
     1561 cttcgggcgc aggcccgtgg tgctggcctc gaacctcggc ctgagcctcg actacctgct
     1621 gatggcgctg gcgccgaacc tgtggtggct gctggtcggg cgcatcgtct ccggcatgac
     1681 ctcggccagc atctcgaccg cctacgccta tatgagcgac gtcaccgcgc cggaaaagcg
     1741 cgcgggcgtg ttcggcctga tgggcgcggc cttcggggtc ggcttcatcc tggggccggg
     1801 catcggcggc gtgctgggcg ggatcgatcc gcgcctgccg ttctatgcgg ccagcgcgct
     1861 cagcatggcc aatttcgcct acggcctgtt cgtgctgccc gaatcgctgc cgaaggcgaa
     1921 gcgcaaggcc ttcagcctgc acgccgccaa tccgctgggc gcggtgaagt tcctggcgcg
     1981 ctcggcgcag gtgctgcgcc tgtcgctgat gtacctgctg at
//
LOCUS       DASVLL010000671         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_157854,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000671 DASVLL010000000
VERSION     DASVLL010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157854"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VF402_10985"
     CDS             <1..614
                     /locus_tag="VF402_10985"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEX7800868.1"
                     /translation="DTYMDKLAIGPGYPQGVVDLDAAPEEDVRALAKAKGVDVSEIVA
                     CVLDRPRHAAIIESLRKAGCRVHLITDGDVAGVIHTAQPETGIDIYMGQGGAPEGVLS
                     CAALKCVGGQFQGRLVFRNNDERARAERLGLTDFERKYTLDDLVSTDAVFIATGVTHG
                     ALLRGVSMEKTARGESFVTTESLVMMSKTHTVRKLSMRRPFKG"
     gene            616..2475
                     /gene="recJ"
                     /locus_tag="VF402_10990"
     CDS             616..2475
                     /gene="recJ"
                     /locus_tag="VF402_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX7800869.1"
                     /translation="MSADAVRPDEAAPFLGVRRSLSGRAWRERLSGAGVTPEALRALT
                     HRLGLLDVAPEDFVGPLARFVAGRGVTAEGLETFLFPTLKALFPDPSCFMDMDKAVGA
                     ILDALQRRANIWIFADYDVDGATSAAQLVRWFRHMGHELKVYVPDRMTEGYGPSPAAF
                     DNLKAQGADLVITVDCGAMAHRALDHAARIGLDVVVVDHHLMREDPPRALAVVNPNRP
                     GCASGQGNLAAAGVVFVLLAALNREADRRGLFAERAKPDLVQWLDLSALGAICDVTAL
                     TGFNRALAAQGLKVMSRRNNPGIRALMEAAGDGAAGAASLMSTFHSGFVIGPRINAGG
                     RVGRSDLGVRLLSTDDPDEARALAQELDELNRLRREVEAEVLEQAVETAEAQGLWPSD
                     DPVLVIARDDWHPGVIGIVAGRLRERWRKPAIVIGIDPVTGLGKGSGRSQAGINLGAA
                     VGAAFEAGILLSGGGHAMAAGLSVDKARLPELRRFINDYVRAHVAEADQVEAVEADAL
                     IALGAATRDFYDRLDLLAPYGQGNPEPLLAFADVRVAYASPLKGGHVRFELADESGAK
                     LRGVMWRAEGTPIGDALLRPAGRMHVLGRLKADDYMGRRGVQLEIEDMAPAVG"
     gene            2548..2622
                     /locus_tag="VF402_10995"
     tRNA            2548..2622
                     /locus_tag="VF402_10995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2581..2583,aa:Glu,seq:ttc)
     gene            2881..3441
                     /locus_tag="VF402_11000"
     CDS             2881..3441
                     /locus_tag="VF402_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX7800870.1"
                     /translation="MSVFDYNKNEAEDSLTISGHVKWFDAAKGYGFIVPQDPAQTSMR
                     DVLLHISSLRDMGRDTAHEGARITCKIAKRAKGWQVTEIESLDAGGDPAHVARSEAPI
                     RSSRLTNENVKVGDLETATIKWFNRTKGYGFVVRGDDPTDIFIHIETLRRYGLEEVQQ
                     GDVIMVRFGEGPKGLVVTEVRPKALA"
BASE COUNT          562 a         1069 c         1232 g          592 t
ORIGIN      
        1 ccgacaccta tatggacaag ctggccatcg ggccgggcta tccgcagggc gtggtcgatc
       61 tcgacgccgc gccggaggag gatgtgcgcg ctctggcgaa ggccaagggc gtcgacgtgt
      121 cggaaatcgt cgcctgcgtg ctggaccgcc cgcgtcacgc cgcgatcatc gaatccctgc
      181 gcaaggcggg ctgccgcgtg catctgatca ccgacggcga cgtggccggg gtcatccaca
      241 ccgcccagcc cgaaaccggc atcgacatct atatgggtca gggcggcgcg cccgagggcg
      301 tcctgtcctg cgcggcgctg aagtgcgtcg gcggccagtt tcagggccgt ctcgtcttcc
      361 gcaacaatga cgagcgcgcg cgcgccgaac gcctcggcct gaccgatttc gagcgcaaat
      421 acacgctcga cgatctggtc agcacggacg ccgtcttcat cgccaccggc gtgacgcacg
      481 gcgcgctgct gcgcggcgtc agcatggaaa agaccgcgcg cggcgaaagc ttcgtcacca
      541 cggaaagcct ggtgatgatg tccaagaccc acacggtgcg caagctgtcg atgcgccgcc
      601 cgttcaaggg ttgagatgag cgccgacgcc gtccgtcccg atgaggccgc gccctttctg
      661 ggggtgcggc gttcgctgag cggacgggcg tggcgcgaac gcctgagcgg cgcgggcgtg
      721 acgcccgaag ccttgcgcgc gctgacgcac cggctgggcc tgctcgatgt ggcgccggag
      781 gatttcgtcg gcccgctggc gcgtttcgtt gccgggcgcg gcgtgacggc ggaggggctg
      841 gagacgtttc tgttcccgac cctgaaggcg ctgtttcccg atccgtcctg cttcatggac
      901 atggacaagg cggtcggcgc gatcctcgac gcgctgcaaa ggcgggcgaa catctggatc
      961 ttcgccgatt acgacgtcga cggcgccacc agcgcggcgc aactggtgcg ctggttccgt
     1021 catatggggc acgagctcaa ggtctatgtg cccgaccgga tgaccgaagg ctacgggcct
     1081 tcgcccgccg cgttcgacaa tctgaaagcg cagggcgccg atctggtcat caccgtcgat
     1141 tgcggcgcga tggcgcaccg ggcgctggac cacgccgcgc ggatcggcct cgacgtggtg
     1201 gtggtcgatc atcacctgat gcgcgaagac ccgccgcgcg cgctggccgt ggtcaatccc
     1261 aaccggccgg gttgcgcgtc cggtcagggc aatctggcgg cggcgggcgt ggtgttcgtg
     1321 ctgctggcgg cgctgaaccg cgaggcggac cggcgcggcc tgttcgcgga gcgcgccaag
     1381 cccgatctgg tgcaatggct ggacctgtcg gcgctggggg cgatctgcga cgtgacggcg
     1441 ctgaccggtt tcaaccgcgc gctggcggcg caggggctga aggtgatgtc gcggcggaac
     1501 aatcccggca tccgcgcgct gatggaggca gcgggcgacg gcgcggcggg cgcggccagc
     1561 ctgatgagca ccttccattc cggcttcgtg atcgggccgc gcatcaatgc gggcggccgc
     1621 gtcgggcgct ccgatctcgg cgtgcgcctt ttgtcgaccg acgatcccga cgaggcgcgg
     1681 gcgctggcgc aggaactgga cgaactgaac cgcctgcgcc gcgaggtcga ggccgaggtg
     1741 ctggagcagg cggtcgagac ggcggaggcg caagggctgt ggccgtccga cgatccggtg
     1801 ctggtgatcg cgcgcgacga ctggcatccg ggcgtgatcg gcatcgtggc gggccgtttg
     1861 cgcgaacgct ggcgcaagcc ggcgatcgtg atcggcatcg atccggtcac tggtctgggc
     1921 aagggatcgg gccgttcgca ggccgggatc aatctcggcg cggcggtcgg ggcggctttc
     1981 gaggccggga ttctgctgtc gggcggcggc cacgccatgg cggcggggct gagcgtggac
     2041 aaggcgcgtt tgccggagct gaggcgcttc atcaacgatt acgtgcgcgc acacgtcgcc
     2101 gaggccgatc aggtcgaggc ggtcgaggcc gacgcgctga tcgccttagg cgccgccacg
     2161 cgcgattttt acgaccggct cgatctgctg gcgccttacg ggcagggcaa tcccgagccg
     2221 ttgctggcct tcgccgacgt gcgcgtggcc tatgcgtcgc cgctgaaagg cgggcatgtg
     2281 cgcttcgagc tggccgacga gagcggcgcc aagctgcgcg gcgtgatgtg gcgcgccgaa
     2341 ggcacgccga tcggcgacgc gctgctgcgt ccggcggggc ggatgcatgt gctggggcgt
     2401 ctcaaggccg acgattatat ggggcggcgc ggcgttcaac tcgaaattga ggacatggcg
     2461 ccggcggtcg gctaggctcg tggaaaagcg gaaaaaaccg cttgcatcgc cgggagggaa
     2521 tgggtatcta gcgcacctct ccgacgcggt cccttcgtct atcggttagg acgtcaggtt
     2581 ttcaacctga aaagaggggt tcgactcccc tagggactgc caccggcggc tttcattctc
     2641 ctgacccgac gcgcttttcg ccgccggatt tgctggccaa gccgatttat ccacagaatt
     2701 gtgttatctg ataccggtcc catcgctggc cgtgatcacg aatctgctcg cggtaagcga
     2761 ttttttaccg taagattcgc aaccgatacg aatgcgcccg ctttttctct cccaaaatgc
     2821 gatatccgtg ttatgataac gcttgtcact tgagggatgc aggcgacgtg gtgaggatag
     2881 atgagcgtgt ttgattataa taaaaacgag gccgaggact ctctgacgat ctccggccat
     2941 gtcaaatggt tcgacgcggc caaggggtat ggatttatcg ttccgcagga tcctgcgcag
     3001 accagcatgc gcgacgtcct gctgcatatt tccagcctgc gcgacatggg ccgcgacacc
     3061 gcgcacgaag gcgcgcgcat cacctgcaag atcgccaagc gggcgaaggg atggcaggtc
     3121 accgagatcg aatccctgga cgccggcggc gatcccgcgc atgtcgcgcg cagcgaggcg
     3181 ccgatccgct cgtcccggct gacgaatgaa aacgtcaagg tcggcgatct ggagacggcg
     3241 acgatcaaat ggttcaaccg caccaagggc tatggcttcg tggtgcgcgg cgacgatccg
     3301 acggatatct tcatccatat cgaaaccctg cggcgttacg gcctcgaaga ggtgcagcag
     3361 ggcgacgtga tcatggtgcg cttcggcgaa ggccccaagg ggctggtggt gaccgaggtg
     3421 cggcctaagg ctctggctta gagcgtttcc ccaaa
//
LOCUS       DASVLL010000672         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_207778,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000672 DASVLL010000000
VERSION     DASVLL010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207778"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..487
                     /locus_tag="VF402_11005"
     CDS             8..487
                     /locus_tag="VF402_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7800871.1"
                     /translation="MAHKNTICLWYDHDAEGAAAFYAQTFPDSAVAAVHRAPTDFPGG
                     KAGQALTVEFTVCGVACLGLNGGSAFRHSEAFSFQIATDDQAETDRYWNAIVGNGGQE
                     NACGWCKDRWGLSWQITPRVLSEAMAQGGAPAQRAFTAMMTMKKIDVAGIEAAVRGA"
     gene            complement(492..695)
                     /locus_tag="VF402_11010"
     CDS             complement(492..695)
                     /locus_tag="VF402_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX7800872.1"
                     /translation="MSEDRIAELEQIVAHQQKTIDELSGQLAEQWKTLDRMGNSLRAL
                     SERLFGLEESASEAAPPTRPPHY"
     gene            complement(692..1558)
                     /locus_tag="VF402_11015"
     CDS             complement(692..1558)
                     /locus_tag="VF402_11015"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX7800873.1"
                     /translation="MTPSDTPRKIAFQGEPGAFSHQASRRWFPDHAPTPYPTFEAAFA
                     AVASGECELGMIPVENSVAGRVSDVHHLLPHSGLKIIGERFLPIEMMLMAVPETKLEN
                     IRVAASHAMALLQCRKALKELNITAEVFHDTAGAARALAQTREPDRAAIAPEVSAELY
                     GLKILRRNMEDARHNTTRFLVLTSADRAVAPPEDEACVTSFVFRVKNIPAALYKALGG
                     FATNGVNMTKLESYIENGRFASTMFYCDVVGRPADAPLKRALEELQFFAVDVEMLGTY
                     PMDPFRQSAGQQ"
     gene            1831..>2049
                     /locus_tag="VF402_11020"
     CDS             1831..>2049
                     /locus_tag="VF402_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX7800874.1"
                     /translation="MSGDLQGNKILGAVLATGLVIMVVKIGSGYIYDQKPPAKPGYFI
                     DVPVAAESGGAAAPVDTPPDWGTVLPTAD"
BASE COUNT          342 a          668 c          690 g          349 t
ORIGIN      
        1 accggtcatg gctcataaga acaccatctg cctgtggtac gatcacgacg cggaaggcgc
       61 ggcggccttc tacgcgcaaa cctttcccga cagcgcggtc gccgccgtgc atcgcgcccc
      121 gaccgacttt cccggcggca aggccggtca ggcgctgacc gtcgaattca ccgtctgcgg
      181 cgtcgcctgt ctgggcctca acggcgggag cgcgttccgg cattccgaag ccttctcgtt
      241 ccagatcgcc acggacgatc aggcggaaac ggaccgctac tggaacgcca tcgtcggcaa
      301 cggcggtcag gaaaacgcgt gcggctggtg caaggacaga tggggcctgt cgtggcagat
      361 cacgccgcgc gtcctgagcg aagcgatggc gcagggcggc gcgcccgccc agcgcgcgtt
      421 caccgccatg atgaccatga agaagatcga cgtcgccggc atcgaggcgg cggtgcgggg
      481 cgcgtaaagg ctcaataatg cggcggcctg gtcggcggcg cggcttcgga cgcgctttcc
      541 tcgaggccga acaggcgctc gctcagcgcg cgcagcgaat tgcccatgcg gtccagcgtc
      601 ttccattgct cggccagttg cccggacagt tcgtcgatcg tcttttgctg atgcgcgacg
      661 atctgttcca actcagcgat gcggtcctcg ctcattgctg ccccgcgctc tgccggaacg
      721 ggtccatcgg ataggtgccc agcatctcga cgtcgacggc gaagaactgc aactcctcca
      781 gcgcgcgttt cagcggcgcg tccgccgggc gcccgaccac gtcgcaatag aacatggtcg
      841 aggcgaagcg cccgttctcg atataggatt ccagcttggt catattgacg ccgttggtgg
      901 cgaagccgcc cagcgcctta tagagcgcgg cgggaatgtt cttgacgcga aagacgaagc
      961 tggtcacgca ggcttcgtcc tcgggcgggg cgaccgcgcg gtccgccgag gtcagcacca
     1021 ggaagcgcgt ggtgttgtgg cgcgcgtcct ccatattgcg gcgcaggatc ttcagcccgt
     1081 acagctccgc cgacacttcc ggcgcgatgg cggcgcggtc cggctcgcgc gtctgggcca
     1141 gtgcgcgcgc cgcgcccgcc gtgtcgtgga acacctcggc ggtgatgttc agctctttca
     1201 gcgccttgcg gcattgcagc agcgccatgg cgtgcgaggc ggcgaccctg atgttttcca
     1261 gcttcgtttc cggcacggcc atcagcatca tctcgatggg caggaagcgc tcgccgatga
     1321 tcttcagccc cgaatgcggc agcaggtgat gcacgtccga gacgcgcccg gccaccgagt
     1381 tctcgaccgg aatcatgccc agctcgcatt cgcccgacgc cacggcggcg aaggcggcct
     1441 cgaaggtcgg atagggcgtc ggcgcatggt cggggaacca gcgccgcgac gcctgatggc
     1501 tgaaagctcc gggctcgccc tgaaaggcga tcttgcgcgg cgtgtcggat ggggtcatgg
     1561 ggaactctgt cgtgcgtatc gtcatcgcca tatacgcttt ttctccggcg ggggaggggt
     1621 aaaacgcgcc atatggctcc cggcgcggca agcgctgcga cgattcgccg cgccccgcgc
     1681 gcttttaccg catccgtcca aaaggcgcct tgcgtcttgg gcggggccgt gccacacaag
     1741 accgacagga taacgtcata atataggcgc cgcggcggat gtcgcggacg cggacgtgca
     1801 gaggaaacag tttaaggggt agccgggccg atgagcggtg atctgcaagg taacaagatt
     1861 ctgggcgccg tgctggcgac cggactggtc atcatggttg tgaagatcgg ctccggctat
     1921 atctacgatc agaagccgcc cgccaagccg ggctatttca tcgacgttcc ggtcgccgcc
     1981 gaaagcggag gcgccgccgc gcccgtcgat acgccgcccg actggggcac ggtgctgccg
     2041 accgccgac
//
LOCUS       DASVLL010000673         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_422999,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000673 DASVLL010000000
VERSION     DASVLL010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422999"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VF402_11025"
     CDS             complement(<1..188)
                     /locus_tag="VF402_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800875.1"
                     /translation="MVWRLTTSLTKKSVSGLVLGTLALLLSAAPAMPQTSDGEDAAAQ
                     LHPYVPPASASAAAPAKAP"
     gene            complement(283..771)
                     /locus_tag="VF402_11030"
     CDS             complement(283..771)
                     /locus_tag="VF402_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX7800876.1"
                     /translation="MSEPTMIIDQMDYAGLTQNALRGVIREALRRAADEGLPGEHHFY
                     ITFLTRANGVSIPADLLARYPYDITIVLQHQYQDLRVADDFFRVTLTFGGAPKILTVP
                     FAAITRFHDPYAQFMLEFEAEEPVAEDEDEPAPAEAAVAEADADAEDAPKIVSLDQFR
                     KK"
     gene            947..1216
                     /locus_tag="VF402_11035"
     CDS             947..1216
                     /locus_tag="VF402_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HEX7800877.1"
                     /translation="MHATADKTKLLARVRRIRGQVEALERALDDEADSAKLLHLIAGA
                     RGAISGLMGEVVEHHIRHHLTLEGSDAIDPAARQALIDVFHAYIK"
     gene            1223..2032
                     /locus_tag="VF402_11040"
     CDS             1223..2032
                     /locus_tag="VF402_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter"
                     /protein_id="HEX7800878.1"
                     /translation="MNDFSHLLQQGAMNAWLFIPSAILLGALHGLEPGHSKTMMAAFI
                     VAVRGTVGQAVLLGLSATVSHTLIVWLVALGGLWLGRNWSGAAVEPWLQVASAGVMLG
                     VGLWMVWRTGAYRRTEHRHGPHDDHHHHEHDGVAAGFAGAGPYADAHEREHAEAIGRR
                     FAGRKATTPQIVLFGLTGGLIPCPASITVLLLCLQLKRFVLGGALVLCFSLGLALTMV
                     ASGVLAALGARHVSRRFKGFGALANRAPYLSGALIVLVALYVGWTGLKALI"
     gene            complement(2077..>2950)
                     /locus_tag="VF402_11045"
     CDS             complement(2077..>2950)
                     /locus_tag="VF402_11045"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7800879.1"
                     /translation="SLLVNLMPAGEYLGEDYHHAGGVPAVVRQLIDQNLIEDALTCTG
                     KSLIENCREAEIEDENVIRAYDKPLKERAGFRVLSGNLFTSAIMKVSVISEEFRQRYL
                     SNPADPEAFEGRAIVFDGPEDYHHRIDDPALGIDEHCILFMRGAGPVGYPGAAEVVNM
                     RCPDYLIKRGVHSLACIGDGRQSGTSGSPSILNASPEAAAGGNLAILKTGDRVRVDLN
                     KGTVNALISDEEIARRRADLEAAGGYQYPASQTPWQAMQRRTIGQMDTGACLEDAVEF
                     QRIAQTMGLPRDNH"
BASE COUNT          442 a          920 c         1059 g          529 t
ORIGIN      
        1 ggggctttcg ccggggcggc ggcgctggcg ctggccggag gcacgtaagg gtgcagttgc
       61 gccgccgcgt cctcgccgtc gctggtctgc ggcatggcgg gcgcggcgct cagcagcagg
      121 gccagcgttc ccagaaccag gccggaaacg gattttttcg taagagaagt cgtcagacgc
      181 cagaccatga ccgccttccg tcaggagttc gccaacagat tccgcccgcg cagtttaggc
      241 gattctgacg ccgggcctag acgcccggac ggcgcgccgg atttattttt tgcggaactg
      301 atccagcgag acgatcttgg gcgcgtcctc cgcgtccgcg tccgcctcgg cgacggccgc
      361 ctccgccggg gcgggttcgt cctcgtcctc cgcgacaggc tcctcggcct cgaattccag
      421 catgaactgg gcgtaggggt cgtggaaacg ggtgatggcc gcgaacggca cggtcaggat
      481 cttcggcgcg ccgccgaagg tcagggtgac gcggaagaag tcgtcggcga cgcgcagatc
      541 ctgatactga tgctgcaaca cgatggtgat gtcgtagggg tagcgcgcca gcaggtccgc
      601 cgggatcgac acgccgttcg cgcgcgtcag gaaggtgatg tagaagtgat gctcgcccgg
      661 cagcccttcg tcggcggccc ggcgcagcgc ttcgcggatg accccgcgca gcgcgttctg
      721 ggtcagcccg gcgtaatcca tctggtcgat aatcatcgtg ggttcggaca tggtgcggct
      781 ttgatcgtcg gggcttggag gcgaaaggga aggcggtccc cgagacttag cacgcgcccg
      841 cggaaacgaa accaaaaaat ccgcccgcgt ataaaaatgc gatggggaaa gcggtggacg
      901 cgtcacgact tatatagtat aggggggtat cctatgggag ggcgacatgc acgcaacggc
      961 cgacaagacc aaactgctgg cgcgcgtgcg ccgcatccgg ggacaggtcg aggcgctgga
     1021 gcgcgcgctg gacgacgagg ccgacagcgc aaagctgctg cacctgatcg cgggcgcgcg
     1081 cggcgcgatt tccgggctta tgggcgaggt ggtcgagcat catattcgcc atcacctcac
     1141 cctggagggg tccgacgcga tcgatcccgc cgcgcggcag gccctgatcg acgtgtttca
     1201 cgcctatatc aaataagccg tcatgaacga tttttcccat ctgctgcaac aaggcgcgat
     1261 gaacgcctgg ctgttcatcc cctcggccat cctgctgggc gcgctgcacg gccttgagcc
     1321 cggccactcc aagaccatga tggcggcgtt catcgtcgcc gttcgcggca cggtggggca
     1381 ggcggtcctg ctggggctgt cggccaccgt gtcgcatacg ctgatcgtgt ggctggtggc
     1441 gctgggcggt ttgtggctgg ggcggaactg gagcggcgcg gcggtcgagc cgtggttgca
     1501 ggtcgcgtcc gccggcgtca tgctgggcgt cggcctgtgg atggtctggc gcacaggggc
     1561 gtatcggcgc accgaacatc gccacggccc tcatgacgat catcaccatc acgaacatga
     1621 cggcgtggcg gcgggcttcg cgggcgccgg gccttacgcc gacgcgcatg agcgcgaaca
     1681 tgccgaggcg atcgggcgcc ggttcgcggg ccgcaaggcg acgacgccgc agatcgtgct
     1741 gttcggcctg accggcgggc ttattccctg cccggcctcg atcaccgtgt tgcttttgtg
     1801 cctgcaactc aagcgtttcg tgttgggcgg cgcgctggtg ctgtgcttca gcctcggcct
     1861 cgccctgacg atggtggcgt ccggcgtgct ggcggcgctg ggcgcgcgcc acgtgtcgcg
     1921 gcggttcaag ggcttcggcg cgctcgccaa ccgggcgccg tatctgtcgg gcgcgctgat
     1981 cgttctggtg gcgctgtatg tcggctggac cgggctgaaa gccctgatct gagacgggaa
     2041 gccccttccc gcgccgggga aggggcgtct gaccggttag tggttgtcgc gcggcaggcc
     2101 catggtctgg gcgatgcgct ggaattccac cgcgtcttcg aggcaggcgc cggtgtccat
     2161 ctggccgatg gtgcggcgct gcatggcctg ccacggggtc tggctggccg ggtattggta
     2221 gccgcccgcc gcttcgagat cggcgcgccg ccgggcgatc tcctcatcgg agatcagcgc
     2281 gttgaccgtg cctttgttga ggtcgacgcg cacgcggtcg ccggttttga gaatggccag
     2341 gttgccgccc gccgccgctt cgggcgaggc gttgaggatc gacggcgagc cggacgtgcc
     2401 ggactggcgc ccgtcgccga tgcaggccag cgaatgcacg ccccttttga tcaggtagtc
     2461 ggggcaacgc atattgacca cttccgccgc gcccggatag ccgaccggcc ccgcgccgcg
     2521 catgaacagg atgcagtgtt cgtcgatgcc gagcgccgga tcgtcgatgc ggtggtgata
     2581 atcctccggc ccgtcgaaca cgatggcgcg gccctcgaag gcttcgggat cggcggggtt
     2641 gctgaggtag cgctgacgga actcctcgga gatcacgctg accttcatga tggccgaggt
     2701 gaacagattg cccgacagca cgcggaagcc cgcgcgttcc ttcagcggct tgtcgtaggc
     2761 gcggatgacg ttctcgtcct cgatctcggc ctcgcggcag ttttcgatga gcgacttgcc
     2821 ggtgcaggtc agggcgtcct cgatcaggtt ctggtcgatc agttgcctga ccacggcggg
     2881 cacgccgccg gcgtggtgat agtcctcgcc cagatattcc ccggcgggca tcagattgac
     2941 cagcagggag
//
LOCUS       DASVLL010000674         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_477666,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000674 DASVLL010000000
VERSION     DASVLL010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477666"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /gene="uxaC"
                     /locus_tag="VF402_11050"
     CDS             complement(<1..1325)
                     /gene="uxaC"
                     /locus_tag="VF402_11050"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX7800880.1"
                     /translation="MMQPLRLHPDRLFPADPGQRAIARRLYDAVKALPIVSPHGHTDP
                     QWFADNEPFSDPASLLIQPDHYVFRMLYSQGVKLEDLGIPARDGSTVERDPRKIWRCF
                     AEHYHLFRGTPSRLWLDFVFADVFGMEVRLEAGSADLYYDRIDMLLRDPAFRPRALFE
                     RFHIEVIATTEGPLDDLRHHRKIRDSGWTGRVVTAYRPDAVSDPETAGFLDNLKAFGE
                     IADEDVGTWRGYLRAHEKRRAAFRDMGATSTDHGHLRATTADLSRVDCEALYARVRAG
                     EASPAEADLFRAQVLTEMARMSIDDGMVMQLHPGVYRSHNPSLLARFGANMGADLPLP
                     GEFVHGLKPLLDRFGADPRLSLILFTLDETTYARELAPLAGHYPALKIGPAWWFNDSP
                     EGMRRFREQVTETAGFYNTVGFNDDTRAFLSIPARHDVARRIDANFLGRL"
     gene            complement(1326..2636)
                     /locus_tag="VF402_11055"
     CDS             complement(1326..2636)
                     /locus_tag="VF402_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7800881.1"
                     /translation="METSQTAEDAARPDTGPPIKPAGYTRWIVCFILFAAVVLSYIDR
                     LVISVLKPHLAAQYHWTETGYADVALYFQLVYGIGFLVAGRIIDRIGAKAGYTLAMIL
                     WTAGHMLHAAVTTTTGMIFARLPLALGEAATYPAALVAVNDWFPKRERALAIGILNAG
                     ANVGAVVTPLIVPAIMLAFGWRMCFIITGALSFVWLAAWLLFYRKPRQHKMASSAEVA
                     WIESDPYVRETRKAPYSKILKTPQAWAYMAGRFLIDPVWWTFLFWLPSFFAKQFGVKD
                     MGFGPPLVAIYVLADLGSIFGGWLSGRFLHAGWTTNRSRKTAMAVMACVVLPVAFAGF
                     APNMWIAVALIGLACAGHQGFSTNLFTVPGDLFPHYASGTVAGLGGLAGACGGMLMAK
                     FAGIILQTTHGDYAPIFMVSSVAYLLALVVVHLICPRWGRANIE"
     gene            complement(2716..>3027)
                     /locus_tag="VF402_11060"
     CDS             complement(2716..>3027)
                     /locus_tag="VF402_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX7800882.1"
                     /translation="AFADVFGVRTGCHGATDLSPVTMAAALHVGRSIPNFGVQEYMRH
                     TDATYEVFPHGYRFDDGLLYAPEAPGLGVDIDEEKAAKYPYQRAYLPVNRLKDGTLFN
                     W"
BASE COUNT          539 a          978 c         1045 g          465 t
ORIGIN      
        1 agacggccta agaagttggc gtcgatgcgg cgggcgacgt cgtggcgggc cgggatcgac
       61 aaaaaggcgc gcgtgtcgtc gttgaagccg accgtgttgt aaaagcccgc cgtctcggtc
      121 acctgctcgc gaaagcggcg catgccttcg ggcgagtcgt tgaaccacca cgccgggccg
      181 atcttcagcg ccggataatg cccggccagc ggcgccagct cgcgcgcata ggtcgtctcg
      241 tccagcgtga acaggatcag gctcaggcgc ggatcggcgc cgaaccggtc gagcagcggc
      301 ttcaaaccgt ggacgaattc gcccggcagc ggcaggtccg cgcccatatt ggcgccgaag
      361 cgcgccagca gcgacgggtt gtggctgcga tagacgccgg ggtgcaactg catcaccatg
      421 ccgtcgtcga tcgacatgcg cgccatctcg gtcaggacct gggcgcggaa caggtcggcc
      481 tcggcggggc tggcctcccc cgcgcgcacg cgggcgtaga gggcttcgca atcaaccctt
      541 gacaggtccg ccgtcgtggc gcgcaaatgg ccgtggtcgg tggaggtcgc gcccatgtcg
      601 cgaaaggcgg cgcggcgctt ttcgtgcgcg cgcaggtaac cgcgccatgt gccgacatcc
      661 tcgtccgcga tctcgccgaa cgccttgagg ttgtcgagga atcccgccgt ttcgggatcg
      721 cttacggcgt cggggcgata ggcggtcacc accctgcccg tccagccgct gtcgcggatt
      781 ttcctgtggt ggcgcagatc gtcgagcggg ccttcggtcg tggcgatgac ctcgatatgg
      841 aaacgctcga acagggcgcg cggccggaag gccggatcgc gcagcagcat gtcgatgcgg
      901 tcgtaataga ggtccgctga tcccgcctcc agccggacct ccatcccgaa cacgtccgcg
      961 aacacgaagt ccagccacag gcgagacggc gtgccgcgga acaggtgata atgttccgcg
     1021 aagcatcgcc atatcttgcg cggatcgcgc tcaaccgttg atccgtcccg cgccgggatg
     1081 ccgaggtctt ccagtttgac gccctgcgaa tagagcatgc ggaagacgta atggtccggc
     1141 tggatcagca ggctggccgg gtcggagaag ggttcgttgt ccgcgaacca ctgcggatcg
     1201 gtgtggccgt gcgggctgac gatcggcagg gccttgaccg cgtcgtacag ccggcgcgcg
     1261 atggcgcgct ggccgggatc ggcggggaac aggcggtcgg gatgcaggcg taagggttgc
     1321 atgatctact cgatattggc ccggccccag cgcgggcaga tgagatgcac gacgaccagc
     1381 gccagcagat aggcgaccga cgagaccatg aagatcggcg cgtaatcgcc gtgcgtggtt
     1441 tgcaggatga tcccggcgaa cttggccatc agcatgccgc cgcacgctcc ggccagcccg
     1501 ccgaggcccg ccaccgtgcc ggacgcatag tgcgggaaca ggtcgcccgg cacggtgaac
     1561 aggttggtcg aaaagccctg atgcccggcg caggccagcc cgatcagcgc gaccgcgatc
     1621 cacatgttcg gcgcgaaccc ggcgaaggcg accggcagca cgacgcaggc catgaccgcc
     1681 atcgccgttt tgcgcgaccg gttcgtggtc cagcccgcat gcaggaaccg cccggacagc
     1741 cagccgccga agatcgaccc cagatcggcc agcacgtaga tggcgaccag cggcggcccg
     1801 aagcccatgt ccttgacgcc gaactgcttg gcgaagaagg acggcagcca gaacaggaag
     1861 gtccaccaca ccggatcgat caggaagcgc ccggccatat aggcccaggc ctgcggggtt
     1921 ttcagaatct tgctgtaggg cgccttgcgc gtctcgcgca catacgggtc ggactcgatc
     1981 cacgccacct cggcggaact ggccatctta tgctggcgcg gcttgcggta gaacaaaagc
     2041 cacgccgcca gccacacgaa ggacagggcg ccggtgatga tgaagcacat gcgccagccg
     2101 aaggccagca tgatggcggg cacgatcagc ggcgtgacca ccgccccgac attggccccg
     2161 gcgttgagga tgccgatggc cagcgcgcgc tcgcgcttgg ggaaccagtc gttgaccgcg
     2221 accagcgccg ccggataggt cgccgcctcg cccagcgcca gcggcaggcg cgcgaagatc
     2281 atgcccgtgg tcgtggtgac ggcggcgtgc agcatatgcc ccgccgtcca caggatcatg
     2341 gccagcgtgt agcccgcctt ggcgccgatg cggtcgatga tccgcccggc gaccagaaag
     2401 ccgatgccgt aaacaagctg gaaatagagc gcgacgtcgg cgtagcccgt ctcggtccag
     2461 tgatactgcg cggccagatg cggcttcagg accgagatca ccagccggtc gatataggac
     2521 agcacgacgg cggcgaacag gatgaagcag acgatccagc gcgtatagcc tgcgggtttg
     2581 atcggcggtc cggtgtccgg gcgcgcggcg tcctccgccg tttgcgacgt ttccatggcg
     2641 tttcctttgc gcttcatgag cgcttgcggg tgacggcctg ttgcgcaggc tctccgaaaa
     2701 aggctcttga cggccctacc agttgaacag cgtgccgtcc ttcagccggt tcaccggcag
     2761 gtaggcgcgc tggtaaggat acttggccgc tttttcctcg tcgatgtcaa cgccgagacc
     2821 gggcgcttcg ggggcgtaca gcaggccgtc gtcgaaacgg tagccgtgcg gaaagacctc
     2881 ataggtcgcg tcggtgtggc gcatatattc ctgaacgccg aagttcggga tcgagcgccc
     2941 gacgtgcagc gccgccgcca tcgtgaccgg cgacaggtcg gtggcgccgt ggcagccggt
     3001 gcgcaccccg aacacgtccg cgaaggc
//
LOCUS       DASVLL010000675         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_207910,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000675 DASVLL010000000
VERSION     DASVLL010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207910"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VF402_11065"
     CDS             <1..188
                     /locus_tag="VF402_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800883.1"
                     /translation="CALISGWELLYQIPAKKCDAAGGWFSYRYRQCAQPLSLPSLTGR
                     KAGSATIDYHDAKKPAA"
     gene            complement(194..1822)
                     /gene="pgi"
                     /locus_tag="VF402_11070"
     CDS             complement(194..1822)
                     /gene="pgi"
                     /locus_tag="VF402_11070"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX7800884.1"
                     /translation="MTLDALKAQAAADSGAVIVDQFKADPDRLARMSLDVAGLHIDVS
                     KQSWTRAGFDAAIAAVENSGLAEAREKMWTGKDINVSEGRAVLHVALRDTDAENLRLR
                     GPEIARDVKSARDAMRDYAGAIRSGEITGATGKRFKTIIHIGIGGSDLGPRLVFQALA
                     PLQPRIDVRFVANVDGSELALALAGLDPAETLVIAVSKTFTTQETLANFLAARDWLVK
                     GLGEDGAKAHMAAVSAAPDKTGAYGVAPDRVFGFRDWVGGRYSLWSAVSLSVIVALGY
                     DVYERLLDGARAMDQHVLTAPLERNAAVWLAAAQLYNRIGLDRPSRAVIPYVHRLRRL
                     AAFLQQLEMESNGKRTSPTGALLDTKTCPVVFGDEGTNAQHAFFQMLHQSEDVVPLEF
                     IAAARNAESSLDMQQKLLSNVIAQGEAFMVGKSLATSEAECRSLGFDDARTAQIAPQK
                     VCPGNKPSSLVLLNELTPESLGALLALYEHKTFVEGVVMGLNSFDQWGVELGKTLGVK
                     VLKDIQASGQGADIAPHDPSTTALIERVKGLDAE"
BASE COUNT          287 a          630 c          635 g          310 t
ORIGIN      
        1 cctgcgcgct gatttcgggc tgggagctgc tgtaccagat tccggcgaag aaatgcgacg
       61 cggcgggcgg ctggttctcg taccgctacc gccagtgcgc ccagcctctg tcgctgccca
      121 gcctgaccgg gcgcaaggcg ggctccgcga ccatcgacta tcacgacgcg aagaagcccg
      181 ccgcttaaag accctattcg gcgtccaaac ccttcacccg ctcgattaac gccgtagtcg
      241 acggatcgtg cggagcgatg tcggcgccct gcccagaggc ctggatgtcc ttcagcacct
      301 tgacgccgag cgtcttgccc agttcgacgc cccactggtc gaagctgttg aggcccatga
      361 ccacgccctc gacgaaggtc ttgtgctcgt aaagcgccag cagcgcgccg aggctttccg
      421 gcgtcagttc gttcagcagc accaggctcg acggcttgtt gccgggacag accttctgcg
      481 gcgcgatctg ggcggtcctg gcgtcatcga agcccaggct gcggcattcc gcctccgagg
      541 tcgccagcga cttgcccacc atgaaggcct cgccctgcgc gatcacgttg gacagcagct
      601 tctgctgcat gtcgagcgag ctttccgcgt tccgcgcggc ggcgatgaat tccagcggaa
      661 ccacatcctc ggactggtgc agcatctgga agaaggcgtg ctgggcgttg gtgccctcgt
      721 cgccgaacac caccgggcag gtcttggtgt ccagaagcgc gcccgtcggc gaggtgcgct
      781 tgccgttcga ctccatctca agctgttgca ggaaggcggc gaggcggcgc aggcgatgca
      841 cgtaaggaat gaccgcgcgg ctggggcggt cgaggccgat gcggttgtaa agctgcgcgg
      901 cggccagcca gacggcggcg ttgcgctcca gcggcgcggt cagcacgtgc tggtccatcg
      961 cgcgcgcgcc gtccagcagg cgctcataga catcgtagcc gagcgcgacg atcaccgaca
     1021 ggctgaccgc cgaccacagc gaatagcgcc cgccgaccca gtccctgaag ccgaacacgc
     1081 ggtccggcgc gacgccatag gcgccggtct tgtccggcgc ggcggaaacg gcggccatgt
     1141 gcgccttcgc gccgtcctcg ccgaggccct tcaccagcca gtcgcgcgcg gcgaggaagt
     1201 tggccagggt ctcctgcgtc gtgaaggtct tggacacggc gatcacgagc gtctccgccg
     1261 gatcgaggcc cgccagcgcc agcgccagct cggagccgtc gacattggcg acgaagcgga
     1321 cgtcgatccg cggctggagc ggcgccagcg cctgaaacac cagacgcggg ccgaggtcgg
     1381 agccgccgat gccgatatgg atgatggttt tgaagcgctt gccggtcgcg ccggtgatct
     1441 cgccggaacg gatcgcgccc gcgtaatcgc gcatcgcgtc gcgcgccgac ttgacgtccc
     1501 gcgcgatctc cggcccgcgc aggcgcaggt tttccgcgtc cgtgtcgcgc agcgcgacgt
     1561 gcagaacggc gcggccttcg gagacgttga tgtccttgcc cgtccacatc ttttcgcgcg
     1621 cttcggcgag gccggaattt tccacggcgg cgatggcggc gtcgaaaccg gcgcgggtcc
     1681 agctctgctt ggagacgtcg atatgcaggc ccgccacgtc gaggctcatg cgcgccagcc
     1741 ggtcggggtc ggccttgaac tgatcgacga tcacggcgcc ggagtcggcg gcggcctggg
     1801 ctttgagggc gtcgagggtc atggcagtct cctgtgatcc aactgtctgt gcgcgatttt
     1861 cg
//
LOCUS       DASVLL010000676         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_262393,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000676 DASVLL010000000
VERSION     DASVLL010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262393"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF402_11075"
     CDS             complement(<1..313)
                     /locus_tag="VF402_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HEX7800885.1"
                     /translation="MPETPDLPPWDDAGRAARRPRWRRAAPVTAFENPWLRLEVSDAV
                     APTGKPARYAMVHFKNRAVAIFPLHDDGTVSLVGQSRFPLDAYSWELPEGGVPYGEDL
                     LD"
     gene            378..992
                     /locus_tag="VF402_11080"
     CDS             378..992
                     /locus_tag="VF402_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX7800886.1"
                     /translation="MTAFDTTPHEFGKPLPQKASADVLEALFNRRSAPAPTLAAPGPS
                     PEEMDLLIRIGLRVPDHGKIGPWRVVRFTPQAKAKVVEKLKALAESRGDRKDAGALIK
                     MSTPPQMLMVVSAPLQPHKIPLWEQQLSAAAVCQNLLIAAGAMGYGANWITDWYAYDA
                     EARAILGVKEDENVAGFVYLGTPTEPPLERERPDYDARISWWEE"
     gene            1032..1322
                     /locus_tag="VF402_11085"
     CDS             1032..1322
                     /locus_tag="VF402_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7800887.1"
                     /translation="MKPSLALAARKADFDALLSRHKAANLRVFGSVARGEDKDGSDLD
                     LLVDALPGATLFDLGGLQEELQDMLGVSVDLLTPGDLPASFRAAVLAEARPL"
     gene            1319..>1607
                     /locus_tag="VF402_11090"
     CDS             1319..>1607
                     /locus_tag="VF402_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX7800888.1"
                     /translation="MTPEKLADYLRHMQEGAQSASGYIAGMEKPAFFADKRTQQAVIM
                     NLVIIGEAATRVMSEFPEFASAHPDIPWRNMRGMRNRIAHGYFDIDLDQVWE"
BASE COUNT          277 a          495 c          558 g          277 t
ORIGIN      
        1 cgtccagcaa gtcctcgccg taggggacgc cgccttcggg cagttcccag ctataggcgt
       61 cgagcggaaa acgcgactgg ccgaccagcg acacggttcc gtcgtcgtgc agaggaaaga
      121 tcgccaccgc gcggttcttg aaatgcacca tcgcgtaccg ggcgggcttg cccgtcgggg
      181 ccaccgcgtc gctgacctca aggcgcagcc acggattctc gaacgccgtc accggcgcgg
      241 cgcggcgcca gcgcgggcgg cgggcggcgc gtccggcatc gtcccagggc ggaaggtcgg
      301 gcgtttcggg catggcgtca ccttgggttt tgcgcggcgg ggtttatata ggaggcgcaa
      361 ccgcacggga tggacctatg accgcatttg acacgacgcc gcatgaattt ggcaagccgc
      421 ttccgcagaa agcttccgcc gacgtgctgg aagcgctgtt caaccgccgc agcgccccgg
      481 cgccgacgct tgcggcgccg gggccttcgc cggaggagat ggatttgctg atccgcatcg
      541 gcctgcgcgt gcccgaccac ggcaagatcg ggccgtggcg cgtcgtgcgc ttcacgccgc
      601 aggccaaggc gaaggtggtc gaaaagctga aagccctggc ggaatcgcgc ggcgacagga
      661 aggacgccgg ggcgctgatc aagatgtcga cgccgccgca gatgctgatg gtggtgtccg
      721 cgccgctcca gccgcacaag attccgctct gggagcagca attgtccgcc gccgcggtgt
      781 gtcagaacct tttgatcgcg gcgggcgcga tgggctatgg cgccaactgg atcaccgact
      841 ggtacgccta cgacgccgaa gcgcgggcga ttctgggcgt gaaagaggac gagaacgtcg
      901 cgggctttgt ttatctcggc acgcccaccg aaccgccgct ggagcgcgaa cggccggatt
      961 acgatgcgcg gatcagttgg tgggaggaat agctctgttc ggctgtcgcg atctgcggta
     1021 aattcgccaa catgaagccg tcgctcgccc ttgctgctcg aaaagccgat ttcgacgccc
     1081 tgctgtcgcg gcataaggcg gcgaacctgc gcgtttttgg gtccgtcgcg cgcggcgagg
     1141 acaaggacgg cagcgatctc gacctgctgg tcgatgcgct gccgggcgcg accctgttcg
     1201 atctcggcgg attgcaggag gaattgcagg acatgctggg cgtcagcgtc gatctgctga
     1261 cccccggcga cctgcccgcg tcgtttcgcg ccgccgtgct ggccgaggcg cggcctctat
     1321 gacgccggag aagctggcgg actatctgcg gcatatgcag gaaggcgcgc agtcggcgtc
     1381 cggctatatt gccggtatgg agaaaccggc ttttttcgcc gacaaacgca cgcaacaggc
     1441 cgtcatcatg aatctggtga tcatcgggga agcggccacg cgcgtcatga gcgaatttcc
     1501 ggaattcgcg tccgcgcatc cggacatccc gtggcggaat atgcgcggca tgcgcaatcg
     1561 gattgcacac ggctatttcg atattgatct ggatcaggtc tgggaga
//
LOCUS       DASVLL010000677         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_477789,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000677 DASVLL010000000
VERSION     DASVLL010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477789"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF402_11095"
     CDS             complement(<1..530)
                     /locus_tag="VF402_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7800889.1"
                     /translation="MRPIKMRLTAFGPFPDTQTVDFRPALGARLFGIYGPTGAGKTSI
                     LDGICFALFGESSGQERQGDDLRSHHATPDVETEVSLIFEVGAKRYHVVRRPRQTVRG
                     KRGDALVERQHWAALYDATDIEVDDIDADNPGVVMEERKVEVVADRMRSILNYSAAQF
                     RQVVLLPQGQFRQLLTA"
     gene            complement(532..1332)
                     /locus_tag="VF402_11100"
     CDS             complement(532..1332)
                     /locus_tag="VF402_11100"
                     /inference="COORDINATES: protein motif:HMM:NF023739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HEX7800890.1"
                     /translation="MSDEHGQVAFSALPYCEIFAAREAFDDVGIASCAHALSAQLAQA
                     RLHVPPGARWVVTAHAFVEGGRITETERPLAQVGGIETVPSAMFEGAAYVALGHLHRA
                     QEAGAAHIRYCGSWMGFGFDEADELKTLSLVELDAAGVARVTELPLVSPRPLRVVTGK
                     LADLVAAGRAPDALGAGALIKAVLTDEGALIDAIGQLRAVYPHVLQLERLNRLAAVGP
                     GAGAAAVDRQDPVKLVAGFLDAVRGRGPDDVETGMIDEALTDLQSEEV"
     gene            complement(1446..1682)
                     /gene="sbcD"
                     /locus_tag="VF402_11105"
     CDS             complement(1446..1682)
                     /gene="sbcD"
                     /locus_tag="VF402_11105"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HEX7800891.1"
                     /translation="MRILHTSDWHLGRTLAQESLLDDQAQLLDQVFAAIVEHRVDVLI
                     IAGDLFDRAVPRKEAVQLFNDFLAAFTPRPARRS"
BASE COUNT          350 a          642 c          588 g          325 t
ORIGIN      
        1 gcagtcagca gctggcggaa ttgtccctgg ggtaacagca ccacctgacg aaactgggcg
       61 gcgctatagt tcaagatcga ccgcattcga tcggccacga cctcaacctt gcgttcctcc
      121 atcacaacgc cggggttatc ggcgtcgatg tcatcgacct caatgtcggt ggcgtcgtag
      181 agggcggccc agtgctggcg ctcgacaagg gcatcgcccc gtttgcctcg gaccgtctga
      241 cgtggccgcc gcacgacatg atatcgcttg gcgccaacct cgaagatcag gctcacctct
      301 gtctcaacat cgggcgtggc atggtgcgaa cgcaggtcat cgccttgccg ctcctggcct
      361 gacgattctc cgaagagagc gaaacaaatg ccatcgagga tcgaggtctt gcccgctcct
      421 gttggcccgt agataccgaa caggcgagcg cccaacgccg ggcgaaaatc gacggtctgc
      481 gtgtcgggga acggtccaaa agccgtcagg cgcattttga tcgggcgcat gtcagacctc
      541 ctcgctctgg aggtcggtca gggcctcgtc gatcatgccc gtctcgacgt cgtcaggccc
      601 cctcccgcgc accgcatcga gaaagccggc caccagtttc accgggtctt gccgatcaac
      661 cgccgctgcg ccggcgcccg ggccgacagc ggcgagccgg ttcaggcgtt ccaactgcag
      721 cacgtgggga tagaccgcgc gcaactgacc gatggcatcg atgagtgcgc cctcgtcggt
      781 caggaccgcc ttgatcagag cacccgcccc gagcgcgtca ggcgcgcgcc cggccgcgac
      841 caggtccgcc agctttccgg tcacgacccg cagcggacgc gggctgacca gcggaagctc
      901 ggtcactcta gccacgccgg cggcgtccag ctccaccagg ctcagagtct ttagctcatc
      961 agcctcatcg aatccgaagc ccatccagga gccgcagtat ctaatgtgag cggcgccggc
     1021 ctcttgcgcg cgatgaaggt gacctagagc gacgtaggcc gcgccctcga acatcgctga
     1081 cgggaccgtc tcgataccgc ccacctgagc caagggccgc tcggtttcgg tgatccgccc
     1141 cccttccaca aaggcgtggg ccgtcaccac ccatcgcgcg cctggcggaa catgcaggcg
     1201 ggcctgcgcc agttgggctg agagggcgtg ggcgcaactg gcgatgccga catcgtcgaa
     1261 cgcctccctc gccgcgaaaa tctcgcagta cggcagcgcc gagaaagcca cctgcccgtg
     1321 ctcatcgctc aagaccaagg cgtggggtcg gtctcgcaaa ggaccccgga tcaacacgcg
     1381 ccgcggatcc tgcagcgccg agttgaagct aacgcggtcg ggggcgtcgt gattacccgc
     1441 gatcgctacg atcgccgcgc cggtctcggc gtaaacgcgg cgaggaaatc gttgaaaagc
     1501 tgaaccgctt ctttgcgcgg caccgcccgg tcgaagaggt cgccggcgat gatcagcacg
     1561 tcgacgcgat gttcgacgat cgctgcgaag acctggtcga ggagctgggc ctggtcgtcg
     1621 aggaggctct cctgcgccag cgtccggccc agatgccaat ctgatgtgtg caagatccgc
     1681 atctgaagcc caacctcccg tctatatagt ttctatatgg actagagctt ggccgtcaac
     1741 tagactctcg gcatgtcgtc tcccacgccg cgatacgcgt atgaagccag gcagcggttt
     1801 ctagacggac ttctcttttg ggaaggccgg gtcaatcggc gcgacctaat cgacacgttc
     1861 cgggtgtcgc agccgcaggc ggctctggac ctcaaggcct acctc
//
LOCUS       DASVLL010000678         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_369638,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000678 DASVLL010000000
VERSION     DASVLL010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369638"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VF402_11110"
     CDS             <1..683
                     /locus_tag="VF402_11110"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800892.1"
                     /translation="YMDQVYQDYLHHKRGLSPAVIWHATTGYYQYMKHDFVGEVKTEP
                     NGSQPILWPFMAKAINYRWDFDGHRTAYTQMLGNPVGWGLGLIGLAGGLILVARRRFR
                     EETPEEARDLDLTEALLAMYAVFMGVHIYLGLQRVMYIYHYFIGLALSFLLAAMVFRI
                     CARRFAPLAKHRFTVLCGISLLLAASFLFYAPLTYHQKMSRAACEARNIPVRLVVCQP
                     VKTHRVAK"
     gene            680..1522
                     /locus_tag="VF402_11115"
     CDS             680..1522
                     /locus_tag="VF402_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX7800893.1"
                     /translation="MTWLDLARRGKTTWWRYLVAWPLAIVAAILTIALLLAAPIWLRW
                     LPASIASDMQSAAHPILFYGGQGLSFAAFLLGFWAAIRLMHGKRFADIRGAWRWRQFF
                     TGAGVWLIALILCTLIDYALRPKGFVLRLNLTPAVALVAALGLLVQTFTEEFVFRGYL
                     SQSVLLATKNPVVTVIVSALLFGALHIPNGLPQAAGAVVFGLAATTMALRTDSLSLSY
                     GVHFVNNLFGAIVVVSANDVFKGAPALITQNTPDLMWSDTVIGAVALLAATVLLLRPW
                     KRAV"
     gene            complement(1519..1743)
                     /locus_tag="VF402_11120"
     CDS             complement(1519..1743)
                     /locus_tag="VF402_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800894.1"
                     /translation="MTVAEAMERIYESLANDNAEIDARIKDLKAALTAEGLKEAVVEP
                     ARLAQNNRQGRKLMQSYFRKKGVTVVFAEG"
     gene            1920..>2265
                     /locus_tag="VF402_11125"
     CDS             1920..>2265
                     /locus_tag="VF402_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7800895.1"
                     /translation="MFANIKRDLTFLKRLLRILRRINRISPDSSDLVCDDFEAVADRY
                     PTRPAIGFEGKTVTYQQLDTLANRYAHWARQRGLKAGDTVALFMPNRIDYIAIWLGLN
                     KIGVITALINNSL"
BASE COUNT          357 a          745 c          708 g          455 t
ORIGIN      
        1 attacatgga tcaggtctat caggactacc tgcatcacaa gcgcggcctg tcgcccgccg
       61 tgatctggca cgcgaccacc ggctattacc agtacatgaa gcacgacttc gtcggcgagg
      121 tgaagaccga accgaacggc tcgcagccga tcctgtggcc gttcatggcc aaggcgatca
      181 actaccgctg ggacttcgac ggccatcgca ccgcctatac gcagatgctg ggcaatccgg
      241 tcggctgggg gctgggcctg atcggtctgg cgggcggcct gatccttgtc gcgcgccgcc
      301 gcttccgcga ggagacgccg gaggaggcgc gagatctcga cctgaccgaa gccctgctgg
      361 ccatgtacgc ggtcttcatg ggcgtgcata tctatctggg cctgcaacgg gtgatgtaca
      421 tctatcacta cttcattggt ctggcgctca gcttcctgct ggcggcgatg gtgttccgca
      481 tctgcgcgcg gcggttcgcg ccgctggcga aacatcgctt caccgtgctg tgcgggatca
      541 gcctgctgct ggcggccagc ttcctgttct acgcgccgct gacctatcat cagaagatga
      601 gccgcgcggc ctgcgaagcg cgcaacatcc ccgtccggct ggtggtctgc cagccggtca
      661 agacacatcg ggtggcgaaa tgacctggct ggacctcgcg cggcgcggca agacgacatg
      721 gtggcgttat ctcgtggcgt ggccgctggc gatcgtggcc gccatcctga cgatcgcgct
      781 gctgctggcc gcgccgatct ggctgcgctg gctgcccgcc agcatcgcca gcgacatgca
      841 gtccgccgcg catccgatcc tgttttacgg cggtcagggc ctgagcttcg ccgccttcct
      901 gctcggcttc tgggcggcga tccggctgat gcacggcaag cgtttcgccg atattcgcgg
      961 cgcatggcgc tggcggcagt ttttcaccgg cgccggggtc tggctgatcg cgctgattct
     1021 atgcacgctg atcgattacg cgctgcgccc caagggcttc gtcctgcgcc tgaacctgac
     1081 gccggccgtc gccctcgtcg cggcgctggg cctgctggtc cagaccttca ccgaggagtt
     1141 cgtctttcgc ggctacctgt cgcaaagcgt gctgctggcg acgaaaaatc cggtcgtgac
     1201 ggtgatcgtc agcgcgcttc tgttcggcgc cctgcatatc cccaacggcc taccgcaggc
     1261 cgcgggcgcg gtcgtgttcg gcctcgccgc cacgacgatg gccctgcgca ccgacagcct
     1321 gtcgctgagc tacggcgtcc acttcgtcaa caacctgttc ggcgcgattg tggtggtgtc
     1381 ggccaacgac gtgttcaagg gcgctccggc gctgatcacg cagaacacgc cggacctgat
     1441 gtggtcggac acggttatcg gcgccgtcgc cctgctggcc gcgacggtcc tgctgctgcg
     1501 cccgtggaag cgtgcggtct agccttcggc gaagacgacc gtgacgccct tcttgcggaa
     1561 ataggactgc atcaacttgc ggccctgacg gttgttctgc gccagccgcg ccggttcgac
     1621 cacggcctcc ttcaggccct ccgccgtcag cgccgctttc aggtccttga tgcgggcgtc
     1681 aatctcggca ttatcgttgg ccagcgactc gtagatgcgt tccatggctt cggcgacggt
     1741 caaggttcat tctcctgtta tcgccgccgg gttacagaaa tgcggcgcgc ggcgcaagcc
     1801 cggcgcgtca gatgcgaggg tcatggtttt tgcgccgccc gcgaaaattg acgctttcgt
     1861 aaagtttgat ttggttatag cctgcttatc aagaaaagac atacggggag tttaagcgca
     1921 tgttcgccaa tatcaaacgt gacctgacct tcctgaaacg cctgctgcgc atcctgcgcc
     1981 ggatcaacag gatttcaccg gactcgtccg atctggtctg cgacgatttc gaggcggtgg
     2041 cggaccgtta tccgacgcgc cccgccatcg gcttcgaggg caagaccgtc acctatcagc
     2101 agctcgacac cctggccaac cgctacgccc actgggcgcg gcagcgcggc ctcaaggcgg
     2161 gcgacactgt ggcgctgttc atgcccaacc gcatcgacta catcgccatc tggctggggc
     2221 tgaacaagat cggcgtgatc accgccctga tcaacaattc gctga
//
LOCUS       DASVLL010000679         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_208299,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000679 DASVLL010000000
VERSION     DASVLL010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208299"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VF402_11130"
     CDS             <1..915
                     /locus_tag="VF402_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800896.1"
                     /translation="RYGATQEVHYALWASHWRPGEPLAGRRLLVCAEQGLGDEILFAS
                     LLPDVLRDLGPEGELTIAVEPRLVSLFARSFPQAQVIAHRTQRVDGLLQRDFPGFDQN
                     LIDLWALMGDFLSLYRARITDFPARNVFLTPDPARIAHWKAVLHGMGDGPKIGVLWKS
                     LKSGARRDPYFAPFAAWCDVLTTPGARFVNLQYGDTAEETRLAADWGAPLLTLPGLDV
                     KDDLDDLAAACRALDLIVAPPNATSNIAAASGAPLWLLTTQRSWPQLGAPNFPWYPTT
                     RVFHTTSPSDWSGVMAEIRAALIERRPA"
     gene            912..2066
                     /locus_tag="VF402_11135"
     CDS             912..2066
                     /locus_tag="VF402_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7800897.1"
                     /translation="MSRPSLGSVDFNDVATRDWLTQARRERDALRLGERPLVLLGAGS
                     VLSRDFVTAMLARGQVAALVDNARAGTSEGGIPVIGDSGVAAVVAQHPDIIGVMCCGS
                     ENAIAHFSRVWAATDRPLLTYFAVLAALPGLVPTGSPLRLIDAFADEARVFAEHAATL
                     DAFMDTESRRTLDALMLYRLTWDQHWLDAVRMPEKSIYFADGLLPIGEDEVLVDGGAY
                     DGDTVRAFSARVGGRYRHIHAFELDPDNARKFAAKSGDMPNVTLHACGLWDGPARVNL
                     KGEIDLGRRIDASLPGQHRLEALDDLDIGPVTLIKLDVEGAEANALRGARQTIARHRP
                     NMAVCTYHRPDDLSVLTGALRDLPGGYRLKLRHHSPILFDSVIYAVWPLP"
BASE COUNT          323 a          729 c          697 g          362 t
ORIGIN      
        1 cgctacggcg cgacgcagga ggtgcattac gccctatggg cgtcgcactg gcgccccggc
       61 gagcctctcg ccggtcgccg cttgctggtc tgcgccgaac aggggctggg cgacgaaatc
      121 ctgttcgcct cgctgctgcc cgacgtgctg cgcgatctgg ggccggaagg cgaactgaca
      181 atcgcggtcg agccgcgtct cgtctccctg ttcgcgcgca gcttccccca ggcgcaggtc
      241 atcgcgcacc ggacgcagcg cgtcgacggc ctgttgcaac gcgacttccc cggtttcgat
      301 cagaacctga tcgacctgtg ggccttgatg ggcgatttcc tcagccttta ccgcgcgcgc
      361 atcacggatt ttccggcgcg aaacgtcttt ctgacgcccg atccggcgcg tattgcgcat
      421 tggaaggccg tgctgcacgg catgggcgac ggacctaaaa tcggcgtcct gtggaaaagc
      481 ctgaagagcg gcgcgcgacg cgatccctat tttgcgccat tcgccgcgtg gtgcgacgtg
      541 ctgaccacgc cgggcgcgcg cttcgtcaat ctgcaatatg gcgatacggc ggaggagacg
      601 cggctggcgg cggactgggg cgcgccgctg ttgaccctgc cggggctgga cgtcaaggac
      661 gatctcgacg atctggcggc ggcgtgccgc gcgctcgatc tgatcgtcgc tccgcccaac
      721 gccaccagca atatcgccgc cgcgtccggc gcgccgctct ggctgttgac gacgcagcgc
      781 agttggccgc aactgggcgc gccgaacttc ccgtggtatc cgacaacgcg cgtcttccat
      841 acgacgagcc cgagcgactg gagcggcgtc atggccgaga tacgcgcggc cctgatcgaa
      901 aggcgtccgg catgagccga cccagcctcg gctccgtcga tttcaacgat gtggcgacac
      961 gcgactggct gacgcaggcg cggcgcgaac gcgacgcctt gcggctgggc gaacgcccgc
     1021 tggtcctgtt gggcgcaggt tccgtgctga gccgtgattt cgtcaccgcc atgctggcgc
     1081 gcggtcaggt cgcggccctc gtggacaatg cacgcgccgg aacgtccgaa ggcggcattc
     1141 ccgtcatcgg cgacagcggc gtcgcggccg tcgtcgcgca gcatcccgac atcatcggcg
     1201 tgatgtgctg cggttcggaa aacgctatcg cgcatttcag ccgggtctgg gcggcgacgg
     1261 atcggccttt gctgacctat ttcgccgtgc tggccgccct gcccggtctg gttccgacgg
     1321 gctcgccgct gcgcctgatc gacgccttcg ccgatgaggc gcgcgtcttc gccgagcacg
     1381 ccgccacgct cgacgccttc atggatactg aaagccgtcg gacgctcgac gcgctgatgc
     1441 tgtaccgcct cacctgggat cagcactggc tggacgccgt gcgcatgccc gaaaagagca
     1501 tctattttgc ggacggcctg ctgccgatcg gcgaggacga ggttctggtc gacggcggcg
     1561 cctatgacgg cgacaccgtc cgcgccttct ccgcgcgcgt cggcgggcgc tatcgtcata
     1621 tccacgcctt tgaactcgac ccggacaatg cgcgaaaatt cgccgctaaa agcggcgata
     1681 tgccgaacgt caccctgcac gcctgcgggc tatgggacgg ccccgcgcgc gttaatctca
     1741 agggcgagat cgacctgggc cgccgcatcg acgccagcct gcccggtcag caccggctgg
     1801 aggcgctcga cgatctcgac atcggtcccg tgaccctgat caagctggat gtcgagggcg
     1861 cggaagccaa cgccctgcgc ggcgcgcggc agacgattgc gcgccaccgt ccgaacatgg
     1921 cggtttgcac ataccatagg ccggacgatc tcagcgtgct gaccggcgcg ctgcgcgacc
     1981 tgccgggcgg ttaccgtctc aaattgcgcc accattcgcc gatcctgttc gacagcgtca
     2041 tctacgcggt ctggcccctc ccgtaaccgg ccgccatcgc cgagcgaggc ggggaatatc
     2101 agtgcgccat a
//
LOCUS       DASVLL010000680         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_369711,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000680 DASVLL010000000
VERSION     DASVLL010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369711"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1470
                     /locus_tag="VF402_11140"
     CDS             142..1470
                     /locus_tag="VF402_11140"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7800898.1"
                     /translation="MAQTYDLIIRNADIINHAGRGKGDIGIVNGKFAAFGDLAQASAA
                     EIFDASGLLAMPGVIDTQVHFREPGLEWKEDLETGSQAAVMGGVVAVFEMPNTNPTTT
                     SHDAFHDKLKRARHRMHCDHAFYVGGTHENIHHLGDLERLPGCCGVKVFMGASTGTLL
                     IADDAGVAAVLGAVRRRATFHSEDEYRLAERRPLAREGDWTSHDFVRDPQSAIQSTQR
                     LVRLAREAGKRIHVLHVTTREEIEFLREHKDVATVELTPQHLTLEAPEAYERLKGLAQ
                     MNPPIRDRYHVEGLWRGVTGGVADVLGSDHAPHTLEEKAKPYPQSPSGMPGVQTLLPV
                     MLNHVANGRLSLERLVDLTSAGAQRVFGVANKGRMAEGYDGDVTLVDLRHKRVLRHAD
                     MRTRSGWTPFDGMEVTGWPKATIIRGKVVMRDDEIVGPSQGEACRFMETL"
BASE COUNT          283 a          535 c          548 g          229 t
ORIGIN      
        1 cggtgcgcgg cccccgatcg cgcgagcaga tccggctgat cgatccgaaa tcgaagcaaa
       61 acgtctgtca tatgttggat ttgccggtcg atagggtcta tgaagccata accgacgcca
      121 tgccaaggaa gggaaccgcc gatggcccag acctatgatc tgatcatccg taacgctgat
      181 atcatcaacc atgcggggcg cggcaagggc gacatcggca tcgtgaacgg caagttcgcg
      241 gccttcggcg atctggcgca ggcctcggcg gcggagattt tcgacgccag cggccttctg
      301 gccatgccgg gcgtgatcga cacgcaggtc cacttccgcg agccgggact ggaatggaag
      361 gaagacctcg aaaccggctc gcaggcggcg gtcatgggcg gcgtcgtggc ggtgttcgag
      421 atgccgaaca ccaacccgac cacgaccagc cacgacgcct ttcacgacaa gctgaagcgc
      481 gcgcgtcacc gcatgcactg cgaccacgcc ttctatgtcg gcggcacgca tgagaacatc
      541 caccacctcg gcgatctgga gcgcctgccg ggctgctgcg gcgtcaaggt gttcatgggc
      601 gcctcgaccg gcacgctgct gatcgcggac gacgcgggcg tggcggcggt gctgggggcg
      661 gtgcgccgcc gcgccacctt ccattccgag gacgaatacc gtctggccga gcgccgcccg
      721 ctggcgcgcg aaggcgactg gaccagccac gacttcgtgc gcgatccgca atcggcgatc
      781 cagtcgaccc agcgcctcgt ccggctggcg cgcgaggcgg gcaagcgcat ccacgtcctg
      841 cacgtcacca cgcgcgagga gatcgaattc ctgcgcgagc acaaggacgt cgccacggtc
      901 gaactgacgc cgcagcacct gacgctcgaa gcgcccgaag cctatgagcg gctgaaaggg
      961 ctggcgcaga tgaacccgcc gattcgcgac cgctatcatg tcgaaggatt gtggcgcggc
     1021 gtcaccggcg gcgtcgccga cgtgctgggc tcggaccatg cgccgcatac gctggaggaa
     1081 aaggccaagc cctatccgca atcgccgtcg ggcatgccgg gcgtgcagac cctgctgccg
     1141 gtcatgctca accacgtcgc caacggccgg ttgtcgctgg agcggctggt cgacctgacc
     1201 tcggcgggcg cgcagcgcgt gttcggcgtg gccaacaagg gccgcatggc cgagggctat
     1261 gacggcgacg tcaccctggt cgacctcagg cacaagcgcg tgctgcgcca cgccgacatg
     1321 cgcacgcgct ccggctggac cccgttcgac ggcatggagg tcaccggctg gcccaaggcg
     1381 accatcatcc gcggcaaggt ggtgatgcgc gacgacgaga tcgtcggccc gtcgcagggc
     1441 gaggcgtgcc gcttcatgga aacgctgtaa gcagcctatt tcgcgggaca ggcccggctc
     1501 acgcgcgcct tttcggcggc gcgggccgcg gcgggaacgt cgcgcgacag gaaaacgtcc
     1561 ggcgacaggg cggtttgcgc cgctccggcc caggg
//
LOCUS       DASVLL010000681         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_532777,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000681 DASVLL010000000
VERSION     DASVLL010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532777"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VF402_11145"
     CDS             <1..1338
                     /locus_tag="VF402_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7800899.1"
                     /translation="ADGVVKLSGAPTVRGSHGDLISMSRNLIITIVVDGKDRETYKAP
                     YGGRLRVEDGAQVKKGQRLVEWDPYTTPIITEVGGKVRFEDLNDGATVREETDEATGI
                     SNRVVVDYRASSRSAELRPAMGLVDEAGAYKKLSNGGDARYMLPVGAILSVGNGDEVK
                     PGDILARMPTEGAKTRDITGGLPRVAELFEARRPKDCAVIAEMDGRVEFGRDYKNKRR
                     IKITPEDGSEAVEFLIPKGKHISVHDGDFIQKGDYIIDGNPDPHDLLRIQGVEALAEY
                     LVNEVQEVYRLQGVPINDKHIEVIVRQMLQKVEILDAGDTGLIKGDHLDKAEVDAENI
                     KAEKRGGRPALTQPVLLGITKASLQTRSFISAASFQETTRVLTEASVQGKIDTLDGLK
                     ENVIVGRLIPAGTGAYLRSLQRIALKRDEQLSHQREEAMEPLPAELLEAPSEA"
BASE COUNT          307 a          499 c          493 g          244 t
ORIGIN      
        1 gcggacggcg tcgtcaagct gtcgggtgcg ccgaccgtgc gcggttcgca cggcgacctg
       61 atctccatgt cgcgcaacct gatcatcacg atcgtggtcg acggcaagga tcgcgaaacc
      121 tacaaggccc cctacggcgg ccgcctgcgc gtcgaggacg gcgctcaggt caagaagggc
      181 cagcgtctgg tcgagtggga tccgtacacc acgccgatca tcaccgaagt cggcggcaag
      241 gtgcgcttcg aggacctcaa cgacggcgcg accgtgcgtg aggaaaccga cgaggcgacc
      301 ggcatctcca accgcgtcgt ggtcgattac cgcgcctcgt cgcgcagcgc cgagctgcgt
      361 ccggccatgg gtctggtcga tgaggccggg gcctacaaga agctgtccaa cggcggcgac
      421 gcccgctaca tgctgccggt cggggccatt ctctccgtcg gcaacggcga cgaggtcaag
      481 ccgggcgaca ttctggcccg tatgccgacc gaaggcgcca agacccgcga catcaccggc
      541 ggtctgccgc gcgtcgccga actgttcgaa gcccgccgtc cgaaggattg cgccgtcatc
      601 gccgaaatgg atggccgcgt cgaattcggt cgcgactaca agaacaagcg ccgcatcaag
      661 atcacgccgg aagacggttc ggaagcggtc gagttcctga tcccgaaggg caagcacatc
      721 tcggtccacg acggcgactt catccagaag ggcgactaca tcatcgacgg caaccccgat
      781 ccgcacgatc tgctgcgcat tcagggcgtc gaggcgctgg ccgaatatct ggtcaacgag
      841 gtgcaggagg tctatcgcct gcaaggcgtg ccgatcaacg acaagcacat cgaagtcatc
      901 gtgcgccaga tgctgcaaaa ggtcgaaatc ctcgatgcgg gcgacaccgg cctgatcaag
      961 ggcgaccatc tcgacaaggc cgaggtcgac gccgaaaaca tcaaggcgga aaagcgcggc
     1021 ggccgtccgg ccctcacgca gcccgtcctg ctcggcatca ccaaggcgtc cttgcagacc
     1081 cgctcgttca tctcggcggc ctcgttccag gaaacgacgc gcgtgctcac cgaagcctcg
     1141 gtgcagggca agatcgacac cctggacggc ctcaaggaga acgtcatcgt gggccgcctg
     1201 atcccggcgg gcaccggcgc ctacctgcgc tcgctgcaac gcatcgccct gaagcgcgac
     1261 gagcaattgt cgcatcagcg cgaagaagcg atggagccgc tgccggcgga actgctggaa
     1321 gcgccttcgg aagcgtaagg ggcgaaagtc tctctatccc gaacccccga agcttcggct
     1381 tcgggggttt tcttttgcgc ttgacgatat atcacccatg ggttacttgt aacctgtagg
     1441 tgatatccat ggagggcgtc atgtccgata acgacaaact gctgcgtcag gccgaggggc
     1501 tggccggttt ccggcgtctg gcgatgatcg tgaacgccgt cag
//
LOCUS       DASVLL010000682         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_478241,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000682 DASVLL010000000
VERSION     DASVLL010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478241"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VF402_11150"
     CDS             <1..711
                     /locus_tag="VF402_11150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7800900.1"
                     /translation="MQGGWCVGRAAPDSPLFVDGKPYGRADARGLFYVGFDRDAPART
                     VVACAGRTESLAIAPVTYDVQRVDGLPPDTVTPQGRELLDRIGREWLLKNRAFASLYD
                     SDDFAKGFIWPLKKFVVSGHFGNQRILNGVPKSPHMGFDMAAPVGTPIHAPQAGRVVL
                     AQPDMFYEGGLIFIDHGQGVISMYLHQSAVLVAADQRVRQGQLIGRVGDRGRATGPHL
                     CWRLKWRDRHLDPSLMVV"
     gene            878..1372
                     /locus_tag="VF402_11155"
     CDS             878..1372
                     /locus_tag="VF402_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7800901.1"
                     /translation="MADYSSLKILLIEDNQHMRSIVTAILGGSGIRHIRQARDGAEGL
                     DMLRQYPADIVLVDFNMEPIDGVEFTRLLRNAPDSANPYLPVVMITGHSERSRVTQAR
                     DSGVNEFVVKPLTARALLGRLDSVIMRPRPFIRCKSYFGPDRRRKSDDAYGGPYRRNT
                     DGVF"
     gene            1475..2758
                     /locus_tag="VF402_11160"
     CDS             1475..2758
                     /locus_tag="VF402_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7800902.1"
                     /translation="MMKPTPLILTLTYIVISASVAAMIFNGGSDFGTAIAALLGCLGV
                     CFAFHTFAAQYLAERRITRDIDSVREAHRLMVDAIDSTQKDMVELAERVQETRMTRSD
                     ELTSEVRMLEDLVKKLGESIEDRLDNWTGGATVVPLRAGDGANPRERQALALIETIRE
                     SLIANRVDLYLQPVVSLPQRKTIFYESFSRLRDASGRVLMPAEYLGVADAEGLVPSID
                     NLLLFRCVQIVRRLAKQDRRIGIFCNISLTSLRDETFFPQFLEFLAENRDLAGSLIFE
                     LGQDAFATRASVEARNMAKLADLGFRFSIDKVTSIDFDLQDLHRSDVRYVKIGASLLL
                     EQLLDVEGKPALKFLKDIHAGDYAGLLARYGIEVIAEKVESEKQVVDILEIDIGYAQG
                     HLFGEPRAIKEQVLAETDPPAGFIKATLPQMRRRA"
     gene            complement(2763..3599)
                     /locus_tag="VF402_11165"
     CDS             complement(2763..3599)
                     /locus_tag="VF402_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7800903.1"
                     /translation="MTNDDAKWKRYAPLLVVISLLVVAITGNVAQTLVNTRHSAYPVG
                     FFTALLQEIASAIVWLSVLPVIVWAFRNLSPFRPFGRVRLLLQAGLIPVVSLTHFVMT
                     RILLVVIYAAAGKAYEFHFNWSYFANDLSKDVLNYILFGLIYLGAERLLGIGATEPPP
                     PPAARAAPVIEVRDGAQVTYVAADDLLWAEAAGNYVELHLASGRKLLMRATLARLAAR
                     LEAADFLRVHRSRIANMAAVSAVENLPGGDAVLKLNGGARIAVSRSYRARVGEALARR
                     VR"
     gene            3770..>4962
                     /locus_tag="VF402_11170"
     CDS             3770..>4962
                     /locus_tag="VF402_11170"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7800904.1"
                     /translation="MLIHILGALLYAHMGCASAHAETSQPESYDAAFFAPFSPQNALD
                     MIQRLPGFTYDAGDESVRGFAEAGGNVLVDGARPVVKSGGLDAALVAIPARQVVRIDI
                     VRKGATGAETSGQNIVANIVLAREAPGVTASATATGVDGSLFGDAALNLTRTLGRFHL
                     TSTTRFDSSGQRSYGFRDQATPAGLRLQHDALRYDTDYPEWTQRLSLDGALGGGRTSV
                     NAMLARARLVETFAFENSGQADRFPDRKERWRGELSADWTRALPRDYDFKLLALTNFT
                     DIDGVSLSQTGGTLATLRTTDRLAYPSHAREAIARATLAKGGDGAWRPEGGVELSWNT
                     LDSRSAMTHFDPDGSPRESSPTHDHVSEARAEAFGALNWLPSPRWALDVGAALETSRI
                     EAGGD"
BASE COUNT          874 a         1692 c         1573 g          823 t
ORIGIN      
        1 atgcagggcg gctggtgcgt cggccgcgcc gcgccggaca gccccctgtt cgtcgacggc
       61 aagccctacg gacgggcgga cgcgcgcggc ctgttctatg tcggtttcga ccgcgacgcg
      121 cccgcgcgga cggtcgtcgc ctgcgccggg cggacggaaa gcctcgccat cgcgccggtg
      181 acctacgacg tgcagcgcgt cgacggcctg ccgccggata cggtcacgcc gcaagggcgg
      241 gaactgctcg accgcatcgg gcgcgaatgg ctgctgaaga accgcgcctt cgccagcttg
      301 tacgacagcg acgacttcgc aaaaggcttc atctggccgc tgaaaaagtt cgtggtttca
      361 ggccatttcg gcaatcagcg catcctgaac ggcgtcccca agagtccgca tatgggcttc
      421 gacatggccg cgcctgtggg tacgccgatt cacgcgccgc aggcgggtcg cgtcgtgctg
      481 gcgcagccgg acatgttcta cgagggcggc ctgatcttca tcgaccacgg ccagggcgtc
      541 atctcgatgt acctgcacca atccgccgtg ctggtcgcgg cagatcagcg cgtccggcag
      601 ggccagttga tcggcagggt cggcgaccgc ggccgcgcga cgggaccgca tctgtgctgg
      661 cggctgaaat ggcgcgaccg ccacctcgac cccagtttga tggtcgtgta aagcccgacg
      721 gcgtcgcaaa ggcgccgcgc tcgcgtaaaa tatcaccctg tttcgcccca aaggcgaaac
      781 tttcgctgga caggccgacg cggctgggcg taacagcggt tcgtttttgc gcgtcccgtt
      841 ttgacacatt ccgccgacct ccctccccct gcacgtcatg gccgattaca gcagcctgaa
      901 aattcttctc atcgaagaca atcagcacat gcgctccatc gtcaccgcca tcctcggcgg
      961 gtcgggcatc cggcatatcc gtcaggcgcg cgacggcgcc gaggggctgg acatgctgcg
     1021 gcaatatccc gccgacatcg tgctggtcga tttcaacatg gagccgatcg acggcgtgga
     1081 gttcacccgc ctgttgcgca acgcgccgga ttcggccaat ccctacctgc cggtcgtgat
     1141 gatcaccggc cattccgagc gctcccgcgt cacgcaggcg cgcgattccg gcgtcaatga
     1201 attcgtcgtc aagccgctga cggcgcgcgc ccttctgggg cggctcgact cggtgatcat
     1261 gcgcccgcgc ccgttcatcc gctgcaagtc ctatttcggc cccgaccgcc gccgcaaaag
     1321 cgacgacgcc tatggcgggc cttaccgccg caacaccgac ggcgtttttt aatatcttgt
     1381 ttataaatct taacgcccgc tatcgcgccg ggcggattca gcccatagtc gcaaagaatc
     1441 ggtacgattc gcccccgatg gctggaaacg ctccatgatg aaaccgaccc cgcttatcct
     1501 gaccctgacc tacatcgtga tcagcgccag cgtcgcggcg atgatcttca acggcggttc
     1561 ggatttcggc acggccatcg ccgcgctgct gggctgcctc ggcgtctgtt tcgccttcca
     1621 caccttcgcc gcgcaatatc tggccgagcg ccgcatcacc agggacatcg acagcgtgcg
     1681 cgaggcgcac cgcctgatgg tcgacgccat cgattcgacg cagaaggaca tggtcgaact
     1741 ggccgagcgc gtgcaggaaa cgcgcatgac gcgctcggac gagctgacct ccgaggtgcg
     1801 gatgctggag gatctggtca agaagctggg cgaaagcatc gaggaccgcc tcgacaactg
     1861 gaccggcggc gccacggtcg tgccgctgcg cgccggcgac ggcgccaacc cgcgtgagcg
     1921 gcaggcgctg gcgctgatcg agacgatccg cgaatccctg atcgccaacc gcgtcgatct
     1981 gtacctgcag ccggtcgtca gcctgccgca gcgcaagacc atcttctacg aaagcttctc
     2041 gcgcctgcgc gacgcctcgg gccgggtgct gatgcccgcc gaatatctgg gcgtggccga
     2101 cgccgagggg ctggtgccgt cgatcgacaa cctgctcctg ttccgctgcg tgcagatcgt
     2161 gcgccgcctc gccaagcagg accgccgcat cggcatcttc tgcaacatct cgttgacctc
     2221 cctgcgcgac gagaccttct tcccgcagtt cctcgaattc ctggccgaga accgcgacct
     2281 ggccgggtcg ctgatcttcg aactggggca ggacgccttc gccacgcgcg cctcggtcga
     2341 ggcgcgcaac atggccaaac tggccgatct cggcttccgc ttctcgatcg acaaggtgac
     2401 cagcatcgat ttcgacctgc aggatctgca ccgctccgac gtgcgctacg tcaagatcgg
     2461 cgcgtccctg ctgctggagc aactgctcga cgtcgagggc aagcccgcgc tgaaattcct
     2521 caaggacatt catgcgggcg actatgcggg gcttctggcg cgctacggca tcgaggtcat
     2581 cgccgagaag gtcgaaagcg agaagcaggt ggtcgacatt ctcgaaatcg acatcggcta
     2641 cgcgcagggc cacctgttcg gcgaaccgcg cgccatcaag gagcaggtgc tggccgaaac
     2701 cgacccgccc gcgggcttca tcaaggccac cctgccgcag atgcgccgcc gcgcctgacc
     2761 gcttacctga cgcgccgcgc caacgcctct ccgacgcggg cgcgatagct gcggctcacc
     2821 gcgatgcgcg cgccgccgtt cagcttcagg acggcgtcgc cgccgggcag gttctcaacc
     2881 gcgctgaccg ccgccatatt ggcgatccgc gaccggtgga cgcgcagaaa atccgccgcc
     2941 tccagacgcg ccgccagccg ggccagcgtg gcgcgcatca gcagtttgcg ccccgaggcg
     3001 agatgcagct cgacatagtt gcccgccgct tcggcccaca gcaggtcgtc ggcggcgaca
     3061 taggtgacct gggcgccgtc gcgcacctcg atcaccggcg cggccctggc ggcgggcggc
     3121 ggcggcggct cggtcgcgcc gatccccaac aggcgctccg cacccagata gatcaatccg
     3181 aacagaatat agttcagcac gtccttgcta agatcgttgg cgaaatagga ccagttgaaa
     3241 tgaaactcgt acgccttgcc cgcggcggcg tagatgacga ccagcaggat gcgcgtcatg
     3301 acgaaatgcg tcagggagac cacgggaatg aggcccgcct gcaagagcag ccgcacccga
     3361 ccgaacggac gaaacggcga caggttgcgg aaggcccaga caatgacggg caagacgctc
     3421 agccagacga tcgcactggc gatttcctgt agcaaggcgg tgaaaaagcc gaccggatag
     3481 gccgaatgcc gggtattgac gagggtttgc gcgacattgc ccgtgatggc gacgaccagc
     3541 aggctgatga ccaccagcaa cggcgcatag cgtttccatt tcgcgtcgtc attcgtcacg
     3601 ccgtttcctc acttcgtcac acccgcgcgc cttttgtccc ggcaggcgcg ggacgcatcc
     3661 cttcgcgctg gcgcatatcg ggtccgcggc tactttggat tatccaaaaa tcatcaggga
     3721 attcagagca gtgttacact tatcccagcg gcaaaaacga cgcaagcaca tgctgatcca
     3781 tatcctgggc gcgttattat atgcccatat gggttgcgca tcggcgcatg cggagacgtc
     3841 tcagccggag tcgtatgacg ccgccttttt cgcgccgttc tcgccgcaga acgcgctcga
     3901 catgatacag cgcctccccg gctttaccta tgacgcgggc gatgaaagcg tgcgcggctt
     3961 cgccgaagcc ggcggcaacg tgctggttga cggcgcgcgc cccgttgtca aaagcggcgg
     4021 cctggacgcc gctctggtcg ccataccggc gcgacaggtg gttcgcatcg acatcgtcag
     4081 aaaaggcgcc acgggcgcgg aaacctccgg gcagaatatt gtggcgaata tcgtgctggc
     4141 gcgggaagcg ccgggcgtta cggcctcggc gaccgccacc ggcgtcgacg gcagcctgtt
     4201 cggcgacgcc gcgctcaacc tgacgcggac cctgggccgc tttcatctga catcgaccac
     4261 acgcttcgat tcgtcaggcc agcgctcgta tggcttccgc gatcaggcca ccccggcggg
     4321 cctgcggctt cagcacgacg ctctgcgcta tgacaccgat tatcccgaat ggacgcagcg
     4381 gctttcgctc gacggcgcgc tcggcggcgg ccggacaagc gtgaacgcca tgctggcgcg
     4441 cgcccggctg gtggagacgt tcgccttcga aaacagcggg caggccgatc gtttcccgga
     4501 ccggaaagag cgctggcgcg gcgaactcag cgccgactgg acgcgggcct tgccgcgcga
     4561 ttacgatttc aaactgctgg cgctgacgaa tttcaccgat atcgacgggg tgtcgctgag
     4621 ccagacgggc gggacgcttg ccaccctgcg gaccaccgac cggctggcct atccctcgca
     4681 tgcccgcgaa gccatcgcgc gcgccacgct ggccaagggc ggcgatggcg catggcggcc
     4741 cgaaggcggc gtcgagctga gctggaatac gctcgacagc cgttcagcga tgacgcattt
     4801 cgacccggac ggttcgccgc gggagtcaag cccgactcac gaccacgtgt cggaagcgcg
     4861 cgcggaggcc ttcggcgccc tgaattggct cccctccccg cgctgggcgc tggatgtggg
     4921 cgccgccctg gaaacctccc gcatcgaggc gggcggcgat ag
//
LOCUS       DASVLL010000683         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_45536, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000683 DASVLL010000000
VERSION     DASVLL010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45536"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1674)
                     /locus_tag="VF402_11175"
     CDS             complement(<1..>1674)
                     /locus_tag="VF402_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7800905.1"
                     /translation="PRGDDAPGLFTALREARAQGAGRALVRLGGFDYEMVVSKLGEDM
                     VLVRAASQGVLGNGLMLRHEGAHALEQAGFLSAPETAPDLPEAQPDAALDAFAAAAPF
                     GAAVVRGPDLFSAAIADFNTAFAVLTEKTPDDLNGMAFGDLVTETSRADLREKLGAGR
                     AGPFDIGLSVTSDRALHIYVTEVAGGFSLYLFDVSDQKKLEQNLVQAQKMQAIGQVAG
                     GMAHDFNNLLTGFKFRNDQLLLNHPLGDPSYEDLNGIRQIIARAEDLVRNLLAFARKQ
                     TVKRVTLNAGEMVSEAEVLLRRLVREDVKLETEYGRNLPNVHVDKGQMEMVMMNLVVN
                     ARDAMRPQGGGKVTIRASALSQDEARMRGWGEAPAQGAALIEVSDTGPGIPPEIVAQV
                     FEPFFTTKPQGEGTGLGLSTVHGIINQAGGHILIDSAPGQGATFRIFLPVWIETEKPD
                     EAPAPEKPARPVPKDLSGVGRILFVEDEQIVRGIAAQLLRQRGYEVLEAADGEEALDI
                     IEAEDGKFDLLISDVIMPGLDGPSMLKKARPLIGDVPVMFISGYAESEFS"
BASE COUNT          244 a          599 c          550 g          281 t
ORIGIN      
        1 gagaattccg attccgcata gcctgagatg aacatcaccg gcacgtcgcc gatcagcggg
       61 cgcgcttttt tcagcatgga cggcccgtcc agccccggca tgatcacgtc cgagatcaac
      121 aggtcgaact tgccgtcctc ggcctcgatg atgtcgagcg cctcctcgcc gtcggcggct
      181 tccagcacct cgtagccgcg ctggcgcagc agttgcgccg cgatgccgcg cacgatctgc
      241 tcgtcctcga cgaacaggat gcgcccgacg ccggacaggt ctttcggcac gggtctggcg
      301 ggcttttccg gcgcgggcgc ctcgtccggc ttttccgtct cgatccacac cggcaggaag
      361 atgcggaagg tcgcgccctg ccccggcgcc gaatcgatca ggatatgccc gcccgcctga
      421 ttgatgatgc catgcacggt cgacaggcca aggccggtgc cctcgccctg cggcttggtg
      481 gtgaagaacg gctcgaacac ctgcgcgacg atctccggcg ggatgccggg gccggtgtcg
      541 ctgacctcga tcagcgccgc gccctgcgcc ggagcctcgc cccagccgcg catccgggcc
      601 tcgtcctgcg acagcgccga ggcgcgaatc gtcaccttgc cgccgccctg cgggcgcatg
      661 gcgtcgcgcg cgttgacgac gaggttcatc atgaccattt ccatctgccc cttgtcgaca
      721 tggacattgg gcaggttgcg gccgtattcg gtctccagct tgacgtcctc gcgcaccaga
      781 cggcgcagca gcacctcggc ctcggacacc atctcgcccg cgttcagcgt cacgcgcttg
      841 accgtctgct tgcgcgcgaa ggccagcagg ttgcgcacca gatcctcggc gcgcgcgatg
      901 atctggcgga tgccgttgag atcctcgtag gacggatcgc cgagcgggtg gttgagcaac
      961 agttgatcgt tgcggaattt gaagccggtc agcaggttgt tgaaatcgtg cgccatgccg
     1021 cccgcgacct ggccgatggc ctgcatcttc tgcgcctgca cgaggttctg ctccagcttc
     1081 ttctggtcgg agacgtcgaa caggtacagg ctgaatccgc ccgccacctc ggtgacatag
     1141 atgtgcagcg cgcggtcgga ggtgacgctg aggccgatgt cgaacggtcc ggcccggccc
     1201 gcgcccagct tttcccgcag gtcggcgcgc gacgtctcgg tgaccagatc gccgaacgcc
     1261 atgccgttca ggtcgtcggg cgtcttttcg gtcagcacgg cgaaggcggt gttgaagtcc
     1321 gcgatcgcgg cggaaaacag gtccggcccc cgcaccaccg ccgcgccgaa cggcgccgcc
     1381 gcggcgaagg cgtcgagagc ggcgtccggc tgcgcttcgg gcaggtccgg cgcggtttcg
     1441 ggcgcgctca ggaaccccgc ctgttccagc gcgtgcgcgc cttcatgccg cagcatcagg
     1501 ccgttgccca gcacgccctg cgacgcggcg cgcaccagca ccatgtcctc gcccagtttg
     1561 gagacgacca tctcgtaatc gaagccgccc agccgcacca gcgcgcgccc ggcgccttgc
     1621 gcccgcgcct cgcgcagggc ggtgaacagg cccggcgcgt cgtcgccgcg cggc
//
LOCUS       DASVLL010000684         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_317942,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000684 DASVLL010000000
VERSION     DASVLL010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317942"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VF402_11180"
     CDS             complement(<1..726)
                     /locus_tag="VF402_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7800906.1"
                     /translation="MTPVAANSDLPPLLARMAETADLVTMALDQLLPHGEGPEARLTE
                     AMRYAALGPGKRLRPFFALETGRLLGADENAVLRAACALECIHAYSLIHDDLPCMDDD
                     DLRRGQLTVHRAYDEATAVLAGDALQSVAFEILAHEDTHDDPLVRCELVKMLARASGA
                     EGMAGGQMIDLLGVHDDIGGVARMQRLKTGALIVYAFQIPLVVADASDRDGLALTHFA
                     QDIGLAYQIADDVLDVEGDPDQMG"
     gene            complement(736..981)
                     /locus_tag="VF402_11185"
     CDS             complement(736..981)
                     /locus_tag="VF402_11185"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7800907.1"
                     /translation="MTDPQTANELSFEAALAQLEKIVAELESGQAPLEKSLELYQKGA
                     ELKALCEARLEAARLQVEKISLNKTGGVEGVVPTSFE"
     gene            complement(978..1607)
                     /locus_tag="VF402_11190"
     CDS             complement(978..1607)
                     /locus_tag="VF402_11190"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX7800908.1"
                     /translation="MRDTTEIHSSRANGPKTGAITLVRHGEPALSRRVKLDWRGYRDW
                     WAAYDRAGLLEGQLPPPVLHDFAREARYIYASPLPRSVQTARAVCQGHGFVELPQLVE
                     APLPPPRLPSFVKFTPNSWGWGFVARAWWWFVDTPGHETRREAQKRAKDMALFLTDKA
                     REGGDVLVLAHGYFNLMISLELKKMGFIQTLEQGFKYWGCRRYELKKTP"
     gene            complement(1730..>2024)
                     /locus_tag="VF402_11195"
     CDS             complement(1730..>2024)
                     /locus_tag="VF402_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEX7800909.1"
                     /translation="DNNWTLDLAVESITSDPVKGTAIVVGARDKLIMPATLRVSFADG
                     TTRDVRLEADAWIRHPTTTVYIEPGRTVVSAEIDPDHRLPDKDRSNNLRKAAR"
BASE COUNT          311 a          687 c          674 g          352 t
ORIGIN      
        1 gcccatctgg tccgggtcgc cctcgacgtc cagcacgtcg tcggcgatct gataggccag
       61 gccgatatcc tgcgcgaaat gggtcagggc cagaccgtcg cggtccgagg cgtcggcgac
      121 caccagcggg atctggaacg catagacgat cagggcgccg gtcttgagcc tttgcatccg
      181 cgccacgccg ccgatgtcgt cgtggacgcc cagaaggtcg atcatctggc cgcccgccat
      241 gccctccgcc cccgaagcgc gcgccagcat cttgaccagt tcgcagcgca ccagcgggtc
      301 gtcgtgcgtg tcctcatgcg ccagaatctc gaaggccacg ctttgcagcg cgtcgcccgc
      361 cagcacggcg gtcgcctcgt cataggcgcg atgcacggtc agttgcccgc ggcgcagatc
      421 gtcgtcgtcc atgcacggca ggtcgtcgtg gatcagcgaa taggcgtgga tgcactccag
      481 cgcgcaggcc gcgcgcagca ccgcgttctc atcggcgccg agcagacggc ccgtctccag
      541 cgcgaagaac ggccgcaacc gcttgcccgg accgagcgcg gcgtagcgca tcgcctcggt
      601 caggcgcgct tccgggcctt cgccgtgcgg caaaagctgg tcgagcgcca tcgtgaccag
      661 atcggcggtc tccgccatgc gcgccagcag cggcggcaga tcggaattgg cggcgacggg
      721 ggtcacgatc tatccttatt caaaagacgt ggggacgacg ccttcgacgc cgccggtctt
      781 gttgagcgaa atcttttcga cctgcaggcg cgccgcttcg aggcgcgctt cgcacagcgc
      841 cttcaattca gcgcccttct gataaagttc caatgacttt tccagcggcg cctgacccga
      901 ttccagttcg gcgacgatct tctcaagctg ggccagcgcg gcttcgaagg acagttcgtt
      961 agcggtttgc ggatcggtca tggcgtcttc ttcagttcgt aacggcggca gccccagtat
     1021 ttgaagccct gctcaagggt ctgtataaag cccatcttct tcagttcaag gctgatcatc
     1081 aggttgaaat aaccgtgcgc cagcaccagc acgtcgccgc cttcgcgcgc cttgtccgtc
     1141 aggaagagcg ccatgtcctt cgcgcgcttc tgcgcttcgc ggcgcgtctc gtggccgggc
     1201 gtgtcgacga accaccacca tgcgcgcgcc acgaagcccc agccccacga gttcggcgtg
     1261 aacttgacga aggacggcag gcgcggcggc ggcagcggcg cctcgaccag ttgcggcagc
     1321 tcgacgaagc cgtggccctg acagacggcg cgggccgtct gcaccgagcg cggcagcggc
     1381 gaggcgtaga tatagcgcgc ctcgcgggcg aaatcgtgca ggaccggcgg cggcaactgg
     1441 ccttccagca ggcccgccct gtcgtaggcc gcccaccagt cgcgatagcc gcgccagtcc
     1501 agcttcacgc gccgcgacag ggccggctcg ccgtgccgca caagggtgat cgcgcccgtc
     1561 ttcggcccgt tcgcccggct tgagtggatc tcagtcgtgt cgcgcacacg gcccccggtt
     1621 cacgactcgc cgcacacgcg cggattcgcc gtcggttgaa acaggggttg ttggtaacgt
     1681 cccggcgccg gggcggcaag tgtttttgtc gcgccgggac cggagcgcgc tatcgggccg
     1741 ccttacgcag gttgttgctc cggtccttgt ccggcaggcg gtggtcgggg tcgatctcgg
     1801 cggagacgac cgtccggccc ggctcgatat acaccgtcgt cgtgggatgg cgaatccagg
     1861 cgtcggcttc gaggcggacg tcgcgcgtcg ttccgtcggc gaagctcacg cgcagggtgg
     1921 cgggcatgat cagtttatcg cgcgcgccga cgacgatggc cgtgcccttg accggatcgc
     1981 ttgtgatgga ctcgacggcg aggtccagcg tccagttgtt gtcg
//
LOCUS       DASVLL010000685         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_208734,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000685 DASVLL010000000
VERSION     DASVLL010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208734"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF402_11200"
     CDS             <1..249
                     /locus_tag="VF402_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX7800910.1"
                     /translation="PPPATPKTSAASKPVPEKKPASEAAKPAAKPAAPTVGAVHTPPK
                     PVIRQAASSSSARAAPASAEPVLKPYVPPAGNGGEGAE"
     gene            249..848
                     /locus_tag="VF402_11205"
     CDS             249..848
                     /locus_tag="VF402_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800911.1"
                     /translation="MRLPAALGLKKAQVGRVISQRGPVNSGLRGGVASRVITPWDWIF
                     APALMSLALTIMLATPFKLFGLYLPEPVSPLILAFAWPLIRPSFIAPLILGLLGLFLD
                     FFWGAPTGFWMLDLMLVYGVLLLARSYIIGQDTVVVFGIFLLAELLFFLFGVLAMTLY
                     AGAVPRLWGVVEQMFATTLLFPFALILLDRYVQTDVKFM"
     gene            856..>2715
                     /gene="mrdA"
                     /locus_tag="VF402_11210"
     CDS             856..>2715
                     /gene="mrdA"
                     /locus_tag="VF402_11210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7800912.1"
                     /translation="MAEPSIVFSDVNERQGTFNRRVFMMGGVVAFGLFALTGRLVHLQ
                     IVQGQKYSKLSAGNQYNFRVIPPPRGEILDRNGKVIAGNRPSFRVMIEVNEVKDIDDT
                     LDQIAYVLPQTVDSRRRILRDINQNQRSVPTIVAADLSWDDFSRVSLYANDIPGVVAD
                     MDEVRAYYYGGAFSHVVGYVSKINDKDMKTELAQPDADPNLLHHPSFRIGKQGIEKAY
                     DRQLRGKAGGKKVEVDARGQVVQEDPEGSIPPTPGENIVLTLDADLQQRAVEVFGQES
                     GAAVLMDCRSGDVLCMSSSPGFDPNLFVSGIPSDVYKLLSTYDHLPLLDKAIGSTFPP
                     GSTFKTMVALAALENGYDPNTIHVCNRVWPWGGHNWRCDKAHGPLNLHQAIVTSCDIY
                     FYQTALWLGGPDKIAAVATRFGLGDIFDIGITGQRAGLVPTVEWKKQYFAKRNPANTR
                     WFPGDTPSIGIGQGFTNVNPLQNCVMVSRLANGQKAINPRLVKSIGGVEQKAGNDAPD
                     LPFNKEHIAFVRAAMADVVSSGTAAGKADLGLGPIKMAGKTGTAQSHTYEKGSTGAYG
                     AVGDWEKRDHAWFVAFAPADDPRYACAVIVQHGGFGGTAAAPKAREIMRLALIK"
BASE COUNT          440 a          922 c          899 g          454 t
ORIGIN      
        1 ccgccgcccg cgacgccgaa gacgagcgcc gcctcgaagc ctgtcccgga gaagaagccc
       61 gcctccgagg cggcgaaacc ggccgccaaa ccggccgcgc cgaccgttgg agccgtgcat
      121 acgccgccca aaccggtcat ccggcaggcc gcgtcgtctt cgtccgcgcg cgccgcgccc
      181 gccagcgccg aaccggtgct gaagccctat gttccgcccg ccggcaatgg cggggagggg
      241 gctgagtgat gcgcctgccc gccgccctag ggctgaagaa agcgcaggtg ggccgcgtga
      301 tctcgcagcg cggcccggtc aattccggcc tgcgcggcgg cgtggcctcg cgcgtgatca
      361 cgccgtggga ctggattttc gcgcccgccc tgatgagtct cgcgctcacc atcatgctgg
      421 cgacgccgtt caagctgttc ggcctctacc tgcccgaacc ggtgtcgccg ctgatcctgg
      481 ccttcgcgtg gccgctgatc cggccgtcct tcatcgcgcc gctgattctg gggctgctcg
      541 gcctgttcct cgactttttc tggggcgcgc cgacggggtt ctggatgctg gacctgatgc
      601 tggtctatgg cgtgctgttg ctggcgcgca gctacatcat cggtcaggac accgtcgtgg
      661 tgttcggcat cttcctgctg gccgagcttc tgttcttcct gttcggcgtg ctggccatga
      721 cgctgtacgc cggggccgtg ccgcgcctgt ggggcgtggt cgagcagatg ttcgccacca
      781 cgctgctgtt cccgttcgcc ctgatcctgc tggaccgcta cgtccagacg gacgtcaagt
      841 tcatgtagcg tcgtcatggc cgaaccctcc atcgtctttt cggatgtgaa cgaacgtcag
      901 gggacgttca accgccgcgt cttcatgatg ggcggcgtgg tggcgttcgg cctgttcgcc
      961 ctgaccgggc ggctggtgca tctgcagatc gtgcaggggc agaaatattc caagctgtcg
     1021 gcgggcaacc agtacaattt ccgcgtcatc ccgccgccgc gcggcgagat tctcgaccgc
     1081 aacggcaagg tgatcgcggg caaccgcccg tccttccgcg tcatgatcga ggtcaacgag
     1141 gtcaaggaca tcgacgacac gctggaccag atcgcctacg tgctgccgca gaccgtggat
     1201 tcgcgccgcc gaatcctgcg cgacatcaac cagaaccagc gttcggtgcc gaccatcgtg
     1261 gccgccgacc tgagctggga cgacttttcg cgcgtcagcc tctacgccaa cgacattccc
     1321 ggcgtggtcg ccgacatgga cgaggtgcgc gcctactatt acggcggcgc cttctcgcac
     1381 gtcgtcggct atgtgtccaa gatcaacgac aaggacatga agaccgagct ggcgcagccc
     1441 gacgccgatc cgaaccttct gcaccacccg tccttccgca tcggcaagca gggcatcgaa
     1501 aaggcctatg accgccagtt gcgcggcaag gcgggcggca agaaggtcga ggtcgacgcg
     1561 cgcggccagg tggttcagga agaccccgaa ggctcgatcc cgccgacgcc gggcgagaac
     1621 atcgtgctga cgctggacgc cgacctgcaa cagcgcgcgg tcgaggtgtt cggtcaggag
     1681 tccggcgccg ccgtgctgat ggactgccgc agcggcgacg ttctgtgcat gagctcctcg
     1741 cccggcttcg atcccaacct gttcgtgtcg ggcatcccca gcgacgtcta taagctgctc
     1801 agcacctacg accacctgcc gctgctggac aaggcgatcg gctcgacctt tccgcccggc
     1861 tcgaccttca agacgatggt ggcgctggcg gcgctggaga acggctacga tcccaacacg
     1921 atccatgtct gcaaccgggt gtggccgtgg ggcgggcaca actggcgctg cgacaaggcg
     1981 cacgggccgc tgaacctgca tcaggccatc gtgacctcgt gcgacatcta tttctaccag
     2041 accgccctgt ggctgggcgg gccggacaag atcgccgccg tggcgacaag gttcgggctg
     2101 ggcgacattt tcgacatcgg catcaccggg cagcgcgccg ggctggtgcc gacggtcgaa
     2161 tggaagaagc agtatttcgc caagcgcaac ccggccaata cgcgctggtt tccgggcgat
     2221 acgcccagca tcggcatcgg gcagggcttc accaacgtca atccgctgca aaactgcgtg
     2281 atggtgtcgc ggctggccaa cggccagaag gcgatcaatc cgcgcctcgt caagtcgatc
     2341 ggcggcgtcg agcagaaggc cgggaacgac gcgcccgacc tgccgttcaa caaggagcat
     2401 atcgccttcg tccgcgccgc catggcggac gtggtgtcca gcggcacggc ggcgggcaag
     2461 gccgacctcg gcctggggcc gatcaagatg gcgggcaaga ccggcacggc gcagtcgcac
     2521 acctatgaga agggcagcac gggcgcctat ggcgcggtcg gcgactggga aaagcgcgac
     2581 cacgcctggt tcgtggcctt cgccccggcg gacgatccgc gctacgcctg cgccgtcatc
     2641 gtgcagcacg gcggcttcgg cggcacggcg gcggcgccca aggcgcgcga gatcatgcgg
     2701 ctggccctga tcaag
//
LOCUS       DASVLL010000686         8467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_533522,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000686 DASVLL010000000
VERSION     DASVLL010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533522"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VF402_11215"
     CDS             complement(<1..374)
                     /locus_tag="VF402_11215"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX7800913.1"
                     /translation="MCGQFDALPPGERLTIARILKVNHAGEYGAIRIYRAQVFVARRR
                     HKDLVAFLDAILTHEIEHCRQFRTAMPARRARPCRAMSFWSFGGYVLGFATALMGENA
                     VMACTRAVERTVHRHLNDQLRFL"
     gene            complement(541..1287)
                     /locus_tag="VF402_11220"
     CDS             complement(541..1287)
                     /locus_tag="VF402_11220"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HEX7800914.1"
                     /translation="MLDRTQFYRFEDFICDTAEARSGDELFDLLKRAAGQYGYDRLIF
                     SAPLDFELPAEWRRWGVYSTYADDWMQEYERRSYAAIDPVLLAAATRDNAFTWAELER
                     TSAYTEAQKRFMLTAEREAGLFNGIGVPIRGRRMLQAGIGLASSAPHDGAEAYRDLLA
                     AFCNQFYLAFKRLYAHTATPPDSKPLSPKETEILTWVAGGKTDDDIAAILGISRNTVD
                     THMRSIFRKLDAGNRVTAVVRALMHGHIHP"
     gene            complement(1343..1915)
                     /locus_tag="VF402_11225"
     CDS             complement(1343..1915)
                     /locus_tag="VF402_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX7800915.1"
                     /translation="MPLLDDISACAVCAAHLPLGPRPVLQAGDPRARILIVGQAPGVK
                     AHTSGTPWDDASGRRLRDWLGVTPRDFYDPARFAILPMAFCYPGRGKGGDLPPRPECA
                     PLWHRPLLATLPDIRLTLLIGGYAQAAYLGARRKATLTETVRAWRDYGDAWPLVHPSP
                     RNQIWLRRNPWFEAETVPALRARVADILSE"
     gene            complement(1924..3036)
                     /locus_tag="VF402_11230"
     CDS             complement(1924..3036)
                     /locus_tag="VF402_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX7800916.1"
                     /translation="MASVTGLSGNEIYCMALKGYTPGELVVGNSVNSMGFLGSVGAGL
                     NNILGGEIPQVTQAIQDGRMHAFSRMAREAQGHGASGVAGVSGNLRGLSGNTEFLFVG
                     SCLHGASGAFFTSAGDAQELYCHMDAGYQPIHHVFGNIAYSMGVGGGLLGALKQLARG
                     EISQYSDVFNKTRHKALERITQAAAADGANAVLGIRTTVLPWMGTHEMVMTGTAMRHP
                     GLPSGQVATSDLTGEELWSMASLGYAPVKLLMSTSIYSLGVVGGIFSAIKSLARGEIS
                     ELTELVHDAREHCISRLKREADELGAQEVIGVKTYVIEIGQGLIEFMAIGTAVRKTAG
                     MGVQTQMLPAQAIVRDKDTWVDGDSGMMLLRQGEGG"
     gene            3218..3397
                     /locus_tag="VF402_11235"
     CDS             3218..3397
                     /locus_tag="VF402_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800917.1"
                     /translation="MPFDPQMYDRRPLRPQPDKTRGHPLSEDDKMGLVLLAVAIVAFG
                     LYTLIVFAGIISGRG"
     gene            complement(3413..3829)
                     /locus_tag="VF402_11240"
     CDS             complement(3413..3829)
                     /locus_tag="VF402_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7800918.1"
                     /translation="MRVRDIMTGDFEVATLHTTLRQASELMRNGGFGFLPVKEGDKLR
                     GAVTDRDIVVRGLADGFGPETPVIHVLSETIVYCFEDDDVREAADLMQREQIRRLAVL
                     NDQKRLVGVLSLGDIARVADDPRLTGAIEAQVARPL"
     gene            complement(3851..4111)
                     /locus_tag="VF402_11245"
     CDS             complement(3851..4111)
                     /locus_tag="VF402_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7800919.1"
                     /translation="MDPHSVGWLTAIIVGALAGWIAEQLMHSNQGLLMNIVLGIVGAI
                     IASALMSVFGIVFAGVIGYLIAGIVGACILIALGRMLRGRTV"
     gene            4322..4801
                     /locus_tag="VF402_11250"
     CDS             4322..4801
                     /locus_tag="VF402_11250"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEX7800920.1"
                     /translation="MRWAGMIVLVSVLAVQAAAARAELGKTAASIDADQRRLAPRGMS
                     ARRLAHANLHEMTMGDGGAVREFTTPDGTVFAVSWRGPRRPNLRQLFGDGYFQRFQAD
                     NVQTGHIRMRRALASTHADFVVRTGGISGAQWGYALLPRLAPAAFDLRQLTATDAQP"
     gene            4798..5640
                     /locus_tag="VF402_11255"
     CDS             4798..5640
                     /locus_tag="VF402_11255"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HEX7800921.1"
                     /translation="MMRRVFALGALLLLAACGGGGGGGSYSSGYSTGGASSSAASSSS
                     SSSSVFANSVPMRVELWPDAATGNVNIGYMTLKICAPGTTTCQTIDHIIVDTGSEGLR
                     IENEALSAAMRAALPETASGTGHLAECYQYVDGYVWGSVRTADLSMGDEQVAAMPLMI
                     IGETGDYAAVPSDCTSGGGNSHDTIADFGGNGIIGIGVLPDDCGNYCQVTTNNGYYYM
                     CPSGGGCTPVTAPVSAQVPNPVARLSVDNNGVIIDLPAVAPLGQASLAGTLYFGIGTR
                     ANTR"
     gene            5652..6071
                     /locus_tag="VF402_11260"
     CDS             5652..6071
                     /locus_tag="VF402_11260"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HEX7800922.1"
                     /translation="MLTTSTDGLLTATYNGQTLNQSFIDSGTNFYGFSDSAITQCTGN
                     LKGFYCPASSLALTATLTGKNAVSANVGFAVDNTEQLNGYDAVLPGLAGDPNGFDNLQ
                     PYSDSFDFGLPFFYGKRVYTAIYGRAAGGYTGPYVAF"
     gene            6111..6965
                     /locus_tag="VF402_11265"
     CDS             6111..6965
                     /locus_tag="VF402_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7800923.1"
                     /translation="MTDYDDVRYRSADARLTLYARDYGGTGTPVLCLHGLTRNSADFA
                     ALARHLSPHYRVIVPDVRGRGLSEYDPQPMHYQPGVYAQDAFALLNHLGVARCVVIGT
                     SMGGIMGMIMGVTAPQRLIGLVLNDVGPELDAAGLARIGAYTGKVEPARDWAEAAARC
                     RASNVQAFPDYGEDDWLAFARRTCRETPDGPAPAYDPGIAVPFKAAEGAAPPDLWGLW
                     DALAAIPALVLRGERSDLLTAATVARMQALHAGLTAVTVPGRGHAPMLDEPAAVAAID
                     AFLREVGA"
     gene            6962..8119
                     /locus_tag="VF402_11270"
     CDS             6962..8119
                     /locus_tag="VF402_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX7800924.1"
                     /translation="MKAVMVGWGHTRFGRLEAGFEQMIHAVTREALADAGLSGADVDA
                     VWLGHFNAGMVPDGFASSMILSADEGLRFKPAVRCENACASGSAALYAAMDAVAAGRV
                     KVALVVGAEKMTAVDGAEVTRALGAASYQAEEAGLSFPQIFAKFALAYQAKYGDPSEA
                     MAHIAVKNHAAAVHNPYAQLRKALELDFCRYASAANPVIAAPLKKTDCSLVSDGAAAV
                     VLVREDMARDFRRAVGFRARAQVNDLLPLSAKDLTAFEGPRRAFDAAYAQAGVGVDDI
                     AFAEAHDCFTIAELLAVEAMGLAAPGQGRGLVIDGGTGRDGKLPVNMSGGLKAKGHPV
                     GATGVSMHVIAARQLTGEAGEMQLPDPGLGMIFNMGGGAVASYVSILERVG"
     gene            complement(8362..>8467)
                     /locus_tag="VF402_11275"
     CDS             complement(8362..>8467)
                     /locus_tag="VF402_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800925.1"
                     /translation="AAAHGGRFEVSEGPGVYDGTGPGLRTALALPPVG"
BASE COUNT         1372 a         2796 c         2929 g         1370 t
ORIGIN      
        1 agaaagcgca actgatcgtt caggtggcga tgcacggtgc gctcgacggc cctggtgcag
       61 gccatgacgg cgttttcccc catcagggcg gtcgcgaagc cgaggacgta gccgccaaag
      121 ctccagaacg acatggcgcg gcaagggcgt gcgcgccgcg cgggcatggc ggtgcggaac
      181 tgacggcaat gctcgatttc atgcgtcaga atggcgtcga gaaacgcaac gaggtcttta
      241 tgccgccgcc gcgccacgaa gacctgcgcc cggtagatgc ggatcgcgcc gtattccccc
      301 gcatgattga ctttcaggat gcgggcgatc gtcagccgtt cgccgggcgg cagcgcatcg
      361 aactgtccgc acataagttt ttctcctatg tgcggacaac tcatgccccc cgccgccccc
      421 cggattcaag gcccggaggc taaaacgggc cttaaatcga aatcgtggcg tcaaacgacc
      481 tgccaaatca aacacatcgc cggtggcggc gccatcacga gaatccgtga ggcggggatt
      541 tcaggggtgg atatgaccgt gcatcagggc gcgcaccacg gcggtgacgc ggttgccggc
      601 gtccagcttg cggaagatcg agcgcatatg ggtgtcgacc gtgttgcggc tgatgccgag
      661 aatcgcggcg atgtcgtcgt cggtcttgcc gcccgccacc caggtcagga tttccgtctc
      721 cttgggcgac agcggcttgc tgtcgggcgg cgtcgcggtg tgggcgtaca gccgcttgaa
      781 ggccaggtag aactggttgc agaaggcggc cagcaggtcg cgatacgcct ccgccccgtc
      841 atgcggcgcc gacgaggcca gtccgatgcc ggcttgcagc atgcggcgcc cgcggatcgg
      901 cacgccgatg ccgttgaaca ggccggcctc gcgctccgcc gtcagcatga agcgcttttg
      961 cgcctcggta taggccgagg tccgctccag ctcggcccag gtgaaggcgt tgtcgcgcgt
     1021 ggccgccgcc agcagcaccg ggtcgatggc ggcgtaggac cggcgctcat attcctgcat
     1081 ccagtcgtcg gcgtaggtgg aatagacgcc ccagcgccgc cattcggcgg gcagctcgaa
     1141 atccagcggc gcggagaaga tcagccggtc atagccgtat tgcccggcgg cgcgtttcag
     1201 aagatcgaac agctcgtcgc cgctgcgcgc ttcggcggtg tcgcagataa aatcctcgaa
     1261 gcggtagaac tgtgtgcggt cgagcatggc gggcctgtga gagaacgcct ttataagcct
     1321 taatgtgtcc cgatggaaac aatcattcgg acagaatatc ggcgacccgc gcgcgcaagg
     1381 cggggaccgt ttcggcctcg aaccacggat tgcgccgcag ccagatctgg ttgcgcggcg
     1441 aaggatggac cagcggccac gcatcgccat agtcgcgcca ggcgcgcacg gtttcggtca
     1501 atgtggcctt gcgccgcgcg cccagatacg cggcctgcgc atagccgccg atcagcaggg
     1561 tcaggcggat gtcgggcaag gtcgccagca gcggcctgtg ccacaaaggc gcgcactccg
     1621 ggcgcggcgg caggtcgccg cccttgccgc gccccggata gcagaaggcc atcggcagga
     1681 tggcgaacct ggccggatcg tagaaatccc gcggcgtcac gcccagccag tcgcgcagcc
     1741 gcctgcccga agcgtcgtcc cacggcgtgc ccgaggtatg cgccttcacg cccggcgcct
     1801 ggccgacgat caggatgcgc gcgcgcggat cgcccgcctg caacaccgga cgcggcccca
     1861 gcggcagatg cgcggcgcag accgcgcagg cggaaatgtc gtcgagcagc ggcaaggcgg
     1921 gggtcagccg ccttcgccct gacgcagcag catcatgccg gaatcgccgt cgacccaggt
     1981 gtccttgtcg cgcacgatgg cctgcgcggg cagcatctgg gtctgcacgc ccatgcccgc
     2041 cgttttgcgc accgccgtgc cgatggccat gaattcgatc aggccctggc cgatctcgat
     2101 cacataggtc ttgacgccga tcacctcctg cgcgcccagt tcgtcggcct cgcgcttcag
     2161 gcggctgatg caatgctcgc gcgcgtcgtg gaccagttcg gtcagctcgg aaatctcgcc
     2221 gcgcgccagc gacttgatcg ccgagaagat gccgccgacc acgcccagcg aatagatcga
     2281 ggtcgacatc agaagcttga ccggcgcgta gccgaggctg gccatcgacc acagttcctc
     2341 gccggtcagg tcggaggtgg cgacctgacc cgacggcagg ccggggtggc gcatggcggt
     2401 gccggtcatg accatttcgt gcgtgcccat ccacggcagc acggtggtgc ggatgcccag
     2461 caccgcgttg gcgccgtccg ccgccgcggc ctgggtgatg cgctccagcg ccttgtgccg
     2521 cgtcttgttg aacacgtcgg aatattgcga aatctcgccg cgcgccagtt gcttgagcgc
     2581 gcccagaagc ccgccgccga cgcccatcga ataggcgatg ttgccgaaga cgtggtggat
     2641 cggctgatag cccgcgtcca tgtggcagta cagttcctgc gcgtcgcccg ccgaggtgaa
     2701 gaaggcgccc gacgcgccgt gcaggcacga gccgacgaac aggaattcgg tgttgccgga
     2761 caggccgcgc agattgccgg acacgcccgc cacgcccgac gcgccgtggc cttgcgcctc
     2821 gcgcgccatg cgcgagaagg cgtgcatccg tccgtcctga atcgcctgcg tcacctgcgg
     2881 gatttcgccg ccgaggatgt tgttcagccc ggcgccgacc gagccgagaa agcccatcga
     2941 attgacgctg ttgccgacca ccagctcgcc gggcgtatag cctttcagcg ccatgcagta
     3001 gatttcgttg cccgataaac ccgtgacgct ggccatgatg cggctccctg cttgcgatgc
     3061 cccgaaggtg tcgccggatg cgcgcgcttg tcaacgcctt tcgacagctt atgctcttat
     3121 cgcattctta cgcggcgggc gccgtccatg cggcgaatct tacgcagggg aggggtatgc
     3181 ttcccggccg gttgggagcg gcgccgtgga gagtctcatg ccctttgatc cccagatgta
     3241 cgaccgccgc ccgctgcgtc cgcagccgga caagacgcgc ggccatccgc tcagcgaaga
     3301 cgacaagatg gggctggtgc tgctggccgt cgcgatcgtg gcgttcggcc tttatacgct
     3361 gatcgtattc gccgggatta tcagcggccg gggttgagcc tctgttgagc ctctaaagcg
     3421 gccgggcgac ctgggcttcg atggcgccgg tcaggcgcgg gtcgtcggcg acgcgcgcga
     3481 tgtcgcccag cgaaagaacc ccgacgaggc gcttctggtc gttgagcacg gccagacggc
     3541 ggatctgctc gcgctgcatc aggtcggcgg cttcgcgcac gtcgtcgtcc tcgaagcaat
     3601 agacgatggt ttcggacagg acatggatga ccggcgtttc gggaccgaag ccgtcggcga
     3661 ggccgcgcac cacgatgtcg cggtcggtca cggcgcccct gagcttgtcg ccctccttca
     3721 ccggcaggaa gccgaagccg ccattgcgca tcagttccga cgcctgtcgc agggtggtgt
     3781 gcaacgtggc gacctcgaaa tcgccggtca tgatgtccct gacgcgcatg gcggcctcct
     3841 tgaaacggcg ttaaacggtc cggccgcgca gcatccggcc cagcgcgatc aggatgcagg
     3901 cgccgacgat gccggcgatc agatagccga tcaccccggc gaacacgatg ccgaacaccg
     3961 acatgagggc gctggcgatg atcgcgccga cgatgcctag cacgatgttc atcagcaggc
     4021 cctgattgga gtgcatgagc tgttcggcga tccagccggc gagggcgccg acaatgatgg
     4081 cggtcagcca tccgacagaa tgcgggtcca tgagactttc ctctctgaca gtccgccgcg
     4141 caaggtgcgg ccagtttacc ttagagtcgc cgtcatcaag ccgatatacg ggtagtttac
     4201 ccgaaaataa ggcgcgcgca aaggaccgga gccgcgaagc cgtccgcgtc acgaagccgc
     4261 ttgcataatt ttgacttggg cctaccatcg acgcacatca cgggggagaa gggcgttgcg
     4321 catgcgctgg gcgggtatga tcgttcttgt gagcgttctg gcggttcagg ccgccgccgc
     4381 ccgcgccgaa ctgggcaaga cggccgcgtc catcgacgcc gatcagcgca ggctggcgcc
     4441 gcggggcatg tcggcgcggc ggctggcgca tgcgaacctg catgaaatga cgatgggcga
     4501 cggcggggcg gtgcgcgaat tcaccacgcc ggacggaacc gtgttcgccg tcagttggcg
     4561 cggcccgcgc cgccccaatc tgcgccagtt gttcggcgac ggctatttcc agcggtttca
     4621 ggccgataac gttcagaccg gccacatccg catgcggcgg gcgctggcct cgacccatgc
     4681 cgacttcgtc gtgcgcacgg gcgggatcag cggcgcgcag tggggctatg cgctgctgcc
     4741 gcgcctcgcg cccgccgcgt tcgacctgcg gcaactgacc gccacggacg cgcagccatg
     4801 atgcggcgcg tgttcgccct gggcgcgctc ctgctgctgg cggcctgcgg cggcgggggc
     4861 ggcggcggga gttacagcag cggctacagc acgggcgggg cttcgtcctc cgccgcgtcc
     4921 tcgtcgtcct ccagcagcag cgtcttcgcc aacagcgtcc ccatgcgcgt cgagctgtgg
     4981 cccgacgcgg ccaccggcaa cgtcaatatc ggctatatga cgctcaagat ctgcgcgccc
     5041 ggtacgacga cctgccagac catcgaccat atcatcgtcg acaccggctc ggagggcctg
     5101 cgcatcgaga acgaggcgct gagcgccgcc atgcgcgccg ccctgccgga gacggcgagc
     5161 gggacgggac atctggcgga atgttatcaa tatgtcgacg gctatgtctg gggttcggtg
     5221 cgcacggccg atctcagcat gggcgacgag caggtcgccg ctatgccgct gatgatcatc
     5281 ggcgaaaccg gcgattacgc cgccgtgccg tccgactgca cgtccggcgg cggcaacagc
     5341 cacgacacca tcgccgattt cggcggcaac ggcatcatcg gaatcggcgt gctgcccgac
     5401 gactgcggca attactgtca ggtaacgacg aacaacggct attattacat gtgcccgtcc
     5461 ggcggcggct gcacgccggt cacggccccg gtctcggcgc aggttcccaa tccggtggcg
     5521 cgcctgagcg tggacaataa cggcgtgatc atcgacctgc ccgccgtcgc gccgctgggg
     5581 caggcgagcc tggccgggac gctctatttc ggcatcggca cgcgcgccaa cacgcgctga
     5641 gcgccgcgaa cgtgctgacc accagcacgg acggcctgtt gaccgcgacc tataacggcc
     5701 agaccctgaa ccagagcttc atcgacagcg gcaccaactt ctacggtttc agcgacagcg
     5761 cgatcacgca atgcacgggc aatctcaagg gcttctattg tccggccagt tcgctggccc
     5821 tgaccgcgac tctgaccggc aagaacgcgg tcagcgccaa tgtcggcttc gcggtcgaca
     5881 ataccgagca gttgaacggc tatgacgccg tgctgcccgg actggcgggc gatcccaacg
     5941 gcttcgacaa tttgcagccc tacagcgaca gcttcgattt cggcctgccg ttcttttacg
     6001 gcaagcgcgt ctatacggcg atctacggcc gcgcggcggg cggctatacc gggccttatg
     6061 tcgccttctg aaaaggttgc aattgcaagc gcccggcgta agctgagcgc atgaccgatt
     6121 acgacgacgt tcgctaccgc agcgccgatg cgcgcctgac cctgtacgcc cgcgattacg
     6181 gcgggacggg aacgccggtg ctgtgcctgc acggcctgac gcgcaacagc gccgatttcg
     6241 ccgcgctggc ccggcatctc agcccacatt accgcgtcat cgtcccggac gtgcgcgggc
     6301 gcggcctgtc ggaatatgat cctcagccga tgcattacca gcccggcgtc tatgcgcagg
     6361 acgcgttcgc gctgctgaac catctcggcg tcgcgcgctg cgtcgtgatc ggcacgtcga
     6421 tgggcggcat catgggcatg atcatgggcg tgacggcgcc gcaaaggctg atcgggcttg
     6481 tgctcaacga tgtcgggccg gagctggacg cggcggggct ggcgcgcatc ggcgcctata
     6541 ccggcaaggt cgagccggcg cgcgactggg ccgaggcggc ggcgcgctgc cgggcaagca
     6601 acgtgcaggc ctttcccgat tatggcgagg acgactggct ggccttcgcg cgccgcacct
     6661 gtcgcgagac gccggacggc ccggcgcccg cctacgatcc cggcatcgcc gtcccgttca
     6721 aggccgccga aggcgcggcg ccgccggatc tgtgggggct gtgggacgcg ctggccgcga
     6781 ttccggccct cgtgctgcgc ggcgaacggt ccgacctgct gacggcggcg accgtggcgc
     6841 ggatgcaggc gcttcatgcg ggcctgacgg ccgtgactgt gccggggcgc ggccatgcgc
     6901 cgatgctgga cgaaccggcg gccgtggccg ccatcgacgc ctttttgcgc gaggtcgggg
     6961 catgaaggcg gtaatggtcg gctgggggca tacgcggttc gggcggctgg aggccggttt
     7021 cgagcagatg atccacgccg tgacgcgcga agccctggcc gatgcgggcc tgagcggcgc
     7081 ggacgtcgac gcggtctggc tcggccattt caatgcgggc atggttccgg acgggttcgc
     7141 gtcgtcgatg atcctgagcg ccgatgaggg cttgcgcttc aagcccgctg tgcgctgcga
     7201 aaacgcctgc gcgtcgggat cggcggcgct gtacgcggcg atggacgccg tcgccgcggg
     7261 ccgggtgaag gtcgcgctgg tcgtcggggc ggaaaagatg acggcggtgg acggcgccga
     7321 ggtgacgcgc gccctgggcg ccgccagcta tcaggccgag gaggcggggc tgagcttccc
     7381 gcagatcttc gccaaattcg ccctggccta tcaggcgaag tacggcgacc ccagcgaggc
     7441 gatggcgcat atcgcggtca agaaccacgc ggcggcggtg cataacccgt atgcgcagtt
     7501 gcgcaaggcg ctggagctgg atttctgccg ctatgcgtcg gcggcgaatc cggtcatcgc
     7561 ggcgccgctg aaaaagaccg actgttcgct ggtgtcggac ggcgcggcgg cggtggtgct
     7621 ggtccgcgag gacatggcga gggatttccg ccgcgcggtc ggctttcgcg cccgcgcgca
     7681 ggtcaacgac ctgctgccgc tgtcggccaa ggatctgacc gcgttcgagg ggccgcgccg
     7741 cgccttcgac gccgcctatg cgcaagccgg cgtcggcgtg gacgacatcg cgttcgccga
     7801 ggcgcacgac tgcttcacca tcgccgaact gctggcggtc gaggcgatgg ggctggccgc
     7861 gccggggcag gggcgcggac tggtcatcga cggcggcacg gggcgcgacg gcaagctgcc
     7921 ggtcaatatg tccggcgggc tgaaggccaa gggccatccg gtcggcgcca ccggcgtctc
     7981 gatgcacgtc atcgccgcca ggcaactgac cggcgaggcg ggcgagatgc aactgcccga
     8041 tcccggtctg gggatgatct tcaacatggg cggcggcgcg gtcgcctctt acgtgtcgat
     8101 cctggagcgg gtgggttaag tccatcatct tcccctctcc cctcattgag gggagaggac
     8161 gagagccgag cgtagcgaag gcgatcgggt gaggggcggc ccgccggtcg agcgcaaacg
     8221 tccgacagcc cctcacccga ggatcggcgg cttcgccttg ctcctcgtcc tctcccctca
     8281 tggaggggag agggggaaga acaacggcgg cgcccttcct tttttgacgt agatcccctt
     8341 gcccgtgagg ggaagtggag cctaccctac cggcggcagc gccagcgcgg tgcgcaggcc
     8401 ggggccggtt ccgtcataga cgcccggccc ttcgctgacc tcgaagcgcc cgccgtgcgc
     8461 ggcggcg
//
LOCUS       DASVLL010000687         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_263533,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000687 DASVLL010000000
VERSION     DASVLL010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263533"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="VF402_11280"
     CDS             complement(<1..1980)
                     /locus_tag="VF402_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7800926.1"
                     /translation="MSRSRIYLLAASALALSAGLAAAPSFAQDQSAADQPTEVVVTGQ
                     RASHRSRLDTLAPVDVIKTDTLTAQGSSELAQGLSRAAPSVNFPRPSGTDATDSIRPV
                     ALRGLSPDQTLVLIDGKRRHASALVNINGSMGRGSSAVDLNAIPESALDRLEVLRDGA
                     SAQYGSDAIAGVVNLHLRQADHGGGVSVSYGEYDTDIKAANSSRTAHDGGTTDINGWA
                     GFKLGSDGFLTLSAEARHRLPTSRGDIDQTVSPAVVTSRYGDPDQKNYAVFANAGKPL
                     NDTWSLYGNANFSSVDSKSAAFYRHAGNAGVVASLYPNGFLPIIEAKSKDLGATIGLR
                     GSIGEWASDFSLTHGDNTLDYYTLNSDNASYGADSQRNFYDGQMKYGQTVFNADISRP
                     FEVGLATPMTVAFGLEARDENYKIKQGDEQSYAIGPLGASGKPGGAQSFGGFQPSNEV
                     DKSRNSVGVYADVAGNFTEKFSYDAAVRYENYSDFGDNTSGKLALRYDFTPSFAIRGS
                     VSSGFRAPSLQQQYFTATSSNLDTTVTPSVIVQTGTFPATSDVAKTLGAQQLKAETST
                     SEALGFVYHKGPFELTVDGYNIRIGNRIVLSENISGSNITPILAPYNVTAARFFMNGV
                     TTTTSGVDAVANYRIPTDRYGRFNLSLAWSNTNTII"
     gene            complement(2343..2930)
                     /gene="rplI"
                     /locus_tag="VF402_11285"
     CDS             complement(2343..2930)
                     /gene="rplI"
                     /locus_tag="VF402_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX7800927.1"
                     /translation="MKVVLLERVENLGTIGDVVGVKDGYARNYLLPRHKALRATAANL
                     KVFEAQKEQIVARNAAAKAAAEKAASELDGASYIIIRQAGDTGQLYGSVNARDVADAV
                     AEAGGKVERNQVVLNTPIKTLGLHQITVRLHAEVAITITVNVARSQDEAERQAAGENV
                     IASQFEEDRLADEAAAADLLEGGAGAAMADAPYEA"
     gene            complement(2943..3221)
                     /gene="rpsR"
                     /locus_tag="VF402_11290"
     CDS             complement(2943..3221)
                     /gene="rpsR"
                     /locus_tag="VF402_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX7800928.1"
                     /translation="MTEDANTPTVGAPVGNAPARRPFFRRRKVCPFSGANAPKIDYKD
                     VKLLQRYISERGKIVPSRITAVSQKKQRELAKAIKRARFLALLPYVVK"
     gene            complement(3237..3608)
                     /gene="rpsF"
                     /locus_tag="VF402_11295"
     CDS             complement(3237..3608)
                     /gene="rpsF"
                     /locus_tag="VF402_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX7800929.1"
                     /translation="MPYYEHVVIARQDISPQQAEALNDQLKALIEENGGHIAKIEYWG
                     LRNLTYRVKKNRKGHYSLLAIDAPAPAVKEMERQLSINEDVIRFMTIRVEELDLELSP
                     ILARRDRPERSERSDRAEASE"
BASE COUNT          586 a         1066 c         1365 g          720 t
ORIGIN      
        1 gatgatcgta ttcgtgttgc tccaggccag cgacaggttg aagcggccgt agcggtcggt
       61 cgggatgcga taattggcca ccgcgtcgac gccgctggtc gtggtcgtca cgccgttcat
      121 gaagaagcgc gcggcggtca cgttgtacgg cgccaggatc ggcgtgatgt tgctgcccga
      181 aatgttttcc gacagcacga tgcggttgcc gatgcggata ttgtagccgt cgacggtcag
      241 ctcgaacgga cccttgtgat agacgaagcc cagggcttcg ctggtcgagg tttcggcttt
      301 gagctgctgg gcgccgagcg tcttggcgac gtcgctggtg gccgggaagg tgccggtctg
      361 gacgatgacg gacggggtga ccgtcgtgtc gagattactg gaggtcgcgg tgaagtactg
      421 ctgttgcagc gacggcgcgc ggaagccgct cgacaccgag ccgcggatcg cgaacgacgg
      481 cgtgaaatcg tagcgcaggg ccagcttgcc ggacgtgttg tcgccgaagt cggaatagtt
      541 ctcatagcgc acggcggcgt catagctgaa cttttcggtg aagttgcccg cgacgtcggc
      601 atagacgccg acgctgttgc ggctcttgtc gacctcgttg gacggctgga agccgccgaa
      661 gctctgcgcg ccgccgggct tgccggacgc gccgagcggg ccgatggcgt aggattgctc
      721 gtcgccctgc ttgatcttgt agttttcgtc gcgcgcttcc agaccgaagg cgacggtcat
      781 cggcgtggcc agaccgacct cgaacgggcg gctgatgtcg gcgttgaaca cggtctggcc
      841 gtacttcatc tggccgtcat agaagttgcg ctgcgaatcg gcgccatagg aggcgttgtc
      901 gctgttgagc gtgtagtagt cgagcgtgtt gtcgccgtgc gtcaggctga aatccgacgc
      961 ccattcgccg atcgagccgc gcaggccgat ggtggcgccc agatccttgg acttggcctc
     1021 gatgatcggc aggaagccgt tgggatagag gctggcgacc acgcctgcgt tgcccgcgtg
     1081 gcggtagaag gccgcgctct tggaatcgac gctgctgaaa ttggcgttgc cgtacagcga
     1141 ccaggtgtcg ttcagcggct tgcccgcatt ggcgaagacg gcgtaattct tctggtccgg
     1201 gtcgccgtag cgcgaggtga cgacggcggg cgaaacggtc tggtcgatgt cgccgcgcga
     1261 ggtcggcaga cgatgacgcg cctcggcgga cagggtcagg aagccgtccg agccgagctt
     1321 gaagcccgcc cagccgttga tgtcggtggt gccgccgtca tgggcggtgc gcgacgaatt
     1381 ggccgccttg atgtcggtgt cgtattcgcc gtaggagacg ctgacgccgc cgccgtgatc
     1441 ggcctgacgc aggtgcaggt tgacgacgcc cgcgatggcg tcggagccgt attgcgccga
     1501 cgcgccgtcg cgcagcactt cgaggcggtc cagcgcgctt tcggggatgg cgttcaggtc
     1561 gaccgccgac gagccgcggc ccatcgagcc gttgatgttg accagagccg acgcatggcg
     1621 gcgcttgccg tcgatcagca ccagggtctg atccggcgac aggccgcgca gggcgaccgg
     1681 gcggatgctg tcggtggcgt cggtgccgga cgggcgcggg aagttgacgc tgggggcggc
     1741 gcgcgacagg ccctgcgcca gttcggacga gccttgcgcg gtcagggtgt cggtcttgat
     1801 gacgtcgacc ggcgccaggg tgtcgagacg cgagcggtgc gaggcgcgct ggccggtgac
     1861 gacgacttcg gtcggctggt cggcggcgga ttggtcctgc gcgaacgacg gcgcggcggc
     1921 aaggcccgcg ctcagggcca gcgcggaagc ggcgagaaga taaatacggg aacgggacat
     1981 gggggaggtc tccagaatgc aataccggcg taaacgcgac acgatcgtgg ccggttgcgt
     2041 cacaattggt cgcattgtgg ccggatttaa agatggtgcg ctgcaataga ttttcgcgcc
     2101 tcaggttaga aaaactatcg ccgggggcat ttcggagcgg aaaagccttt gaatttatga
     2161 tgttccgatg acgtgaccga tctccttggc cgcgtggcgc gcggcgggga cgtgatcaca
     2221 taggcggctc agtgtggcga aatcatcaca aaaataagac aggccggaaa gacgaagccc
     2281 cggaaggcgg aaccttccgg ggcttgttct cagacgtagg atgcagcgtt tgcgcgccgc
     2341 gattacgctt cgtagggcgc gtccgccatg gcggcgccgg cgccgccttc gagcaggtcg
     2401 gcggcggcgg cttcgtcggc gagacggtct tcctcgaatt gcgaggcgat gacgttctca
     2461 ccggcggcct gacgctcggc ttcgtcctgc gagcgggcca cgttcaccgt gatggtgatc
     2521 gcgacctcgg cgtgcaggcg caccgtgatc tgatgcagac ccagggtctt gatcggcgtg
     2581 ttcaggacga cctgattgcg ctcgaccttg ccgccggctt cggccacggc gtccgccacg
     2641 tcgcgggcgt tgaccgaacc gtaaagctgg ccggtgtcgc cggcctgacg gatgatgatg
     2701 tacgacgcgc cgtccagttc cgaggcggcc ttttcggcgg cggccttggc ggcggcgttg
     2761 cgggccacga tctgctcctt ctgggcctcg aagaccttta ggttggcggc ggtggcgcgc
     2821 agcgccttgt ggcgcggcag caggtagtta cgggcgtagc cgtccttcac gccgacgacg
     2881 tcgccgatcg tgccgaggtt ttccacgcgt tccagcaaaa caactttcat gatccgtact
     2941 ccttacttca cgacgtaagg cagcagggcc agaaagcggg cgcgcttgat ggccttggcc
     3001 agttcgcgtt gcttcttctg gctgacggcc gtgatgcgcg agggaacgat cttgccgcgc
     3061 tcggagatgt agcgttgcag gagcttgacg tccttatagt cgatcttcgg ggcgttggcg
     3121 ccggagaacg ggcacacctt gcggcggcgg aagaagggac gacgggcggg ggcgttgccg
     3181 acgggcgcgc cgacggtggg ggtgttggcg tcttcagtca tagtgaaaac cctcccttat
     3241 tccgaggctt cggcgcggtc cgaacgctcg gagcgttccg ggcggtcgcg gcgggccagg
     3301 atgggcgaca gttcgaggtc gagttcctcg acgcggatgg tcatgaagcg gatgacgtct
     3361 tcattgatcg agagctggcg ctccatttcc ttcacggccg gggccggggc gtcgatggcc
     3421 agcagggaat agtggccctt gcggttcttc ttgacgcggt aggtcaggtt gcgcaggccc
     3481 cagtattcga tcttggcgat atggccgccg ttttcctcga tcagggcttt gagctggtcg
     3541 ttgagggctt ccgcctgttg cggcgaaatg tcttgtcgcg cgatgacgac gtgttcgtag
     3601 taaggcatct tatcctcttc cgttgcgccg ggcggcacgg ccttgagcgg aagtctcagg
     3661 gccatgatgg atcggtggtt cgcacgatgc gagccgaccg cccggcaggg aagcgcgccc
     3721 tttacaggat cggacgg
//
LOCUS       DASVLL010000688         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_159650,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000688 DASVLL010000000
VERSION     DASVLL010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159650"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1972
                     /locus_tag="VF402_11300"
     CDS             104..1972
                     /locus_tag="VF402_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX7800930.1"
                     /translation="MKKALSTALLASAASMLALCAASAQAQSYTRLVSFGDSLSDNGN
                     LYTATGQPPAPYNHRFTNDRTWAEYLSGTMLGYFTPASAVNSGSIDLAWGGARADNAA
                     NSNGAIPSSYQQIQTFAGEGGQFAAGDVASLWAGANDLFQALPAAASNPATAQAYMTG
                     AAQTAAGYVATEAGMLAKTGAKTILVLNLPDLGATPQFNADANASALSSYSGETFNAA
                     LHAGLLQVAGAATGSDIVEVDIFSALQTIEANPQAYGFTDVTHACIYVAACVTGTADT
                     QNGYLFWDGVHPTAGGHKLIAALTAQYLYTPSLTAGVGLFADESYETRRSTANDMAGL
                     LHAAHDGGFFAQVVGDDGQRQRPVSVQDGIGGETITDDAKAYDYRQAGLRAGFMHPLN
                     ASSSLGFGGTVVSGDTKGFMISARTLDISLDAAYDWRSGPMFVTASAGAGAGSYSDYR
                     RATLFGPYTNGRSHVGTSAYSAALQAGIDHKLGAWTVTPLARLSYVTASMQGFDEFGT
                     IAAVGFDDRKVSSTNGALELQAAGQIAPTVRVDGVIGYEGVLSGDEGDLRGRLLNNTA
                     QPFTTAMGDVESPGVLAGIGVSATVRGFDLGIRYRGTFGSHSQKSQSALLSVSKKF"
     gene            complement(2036..2266)
                     /locus_tag="VF402_11305"
     CDS             complement(2036..2266)
                     /locus_tag="VF402_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800931.1"
                     /translation="MAISGDYPHPVQVNGFLCKNCTDVDYAKKHIDPAHPRSGPYGVD
                     AASDPTQKAALDPATAPTAQLTAPGRMLDLRA"
     gene            complement(2321..2467)
                     /locus_tag="VF402_11310"
     CDS             complement(2321..2467)
                     /locus_tag="VF402_11310"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX7800932.1"
                     /translation="MIGTSGQTADVCFASTAMRRIGLIHCVIAYLFNATVLALMINIS
                     ASLL"
BASE COUNT          413 a          807 c          841 g          414 t
ORIGIN      
        1 cggaaatctt gtcaaacagc ggcggaaggc ggatgctttc cgttgacgga aaggtaagct
       61 ttgcgcctgc ccgtcaagcc ataaaaatat tgacagggtt gttatgaaaa aagctctttc
      121 cacggcgctg ctggcgtcgg ccgcctccat gctggccctt tgcgcggcgt cggcgcaggc
      181 gcaatcctat acgcgtctgg tcagcttcgg cgacagcctg agcgacaacg gcaatctcta
      241 taccgcgacg ggacagccgc ccgcgccgta caatcaccgc ttcaccaacg accggacctg
      301 ggcggaatat ctgtccggga cgatgctcgg ctatttcacc ccggcttcgg cggtcaattc
      361 gggcagcatc gacctcgcct ggggcggggc gcgcgccgac aacgcggcca acagcaacgg
      421 cgccatcccg tccagctatc agcagattca gaccttcgcg ggcgagggcg gccagttcgc
      481 cgccggcgac gtcgccagcc tgtgggccgg ggccaacgac ctgtttcagg cgctgccggc
      541 cgccgcgtcc aacccggcca ccgcgcaggc ctacatgacc ggcgcggcgc agacggcggc
      601 gggctatgtc gcgaccgagg cggggatgct ggccaagacg ggcgcgaaga ccattctggt
      661 gctgaacctg cccgacctcg gcgccacgcc gcagttcaac gccgacgcca acgcctcggc
      721 cctgtccagc tattcgggcg agacgttcaa cgccgccctg cacgcgggcc tgttgcaggt
      781 cgcgggcgcg gccacgggca gcgacatcgt cgaggtcgac atcttctcgg ccctgcaaac
      841 catcgaggcc aatccgcagg cctacggctt cacggacgtg acccacgcct gcatctatgt
      901 cgcggcctgc gtgaccggga cggcggatac gcagaacggc tatctgttct gggacggcgt
      961 gcatccgacc gcgggcgggc ataagctgat cgccgcgctg acggcgcaat atctctatac
     1021 gcccagcctg accgccgggg tcggcctgtt cgcggacgaa agctacgaaa cgcgccgttc
     1081 gacggcgaac gacatggccg gcctgctgca cgccgcgcac gacggcggct ttttcgcgca
     1141 ggtcgtcggc gacgacggcc agcgccagcg tccggtcagc gtgcaggacg gcatcggcgg
     1201 cgagacgatc accgacgacg ccaaggccta tgattaccgg caggcgggcc tgcgcgcggg
     1261 cttcatgcat ccgctgaacg ccagctccag cctcggtttc ggcggtacgg tggtcagcgg
     1321 cgacaccaag ggcttcatga tcagcgcgcg cacgctggat atcagcctcg acgccgccta
     1381 tgactggcgt tcgggaccga tgttcgtgac cgcttcggcg ggcgcgggcg cgggcagcta
     1441 ttccgactat cggcgcgcga ccctgttcgg gccttatacg aacgggcgca gccatgtcgg
     1501 gacttcggcc tattccgccg ccttgcaggc cgggatcgac cacaagctcg gcgcctggac
     1561 ggtcacgccg ctggcgcgcc tgtcctatgt gacggccagc atgcagggct tcgacgagtt
     1621 cggcacgatc gcggcggtcg gtttcgacga ccgcaaggtc agttcgacca acggcgcgct
     1681 ggagcttcag gccgccgggc agatcgcccc gactgtgcgc gtcgacggcg tgatcggtta
     1741 cgagggcgtg ctgagcggcg acgagggcga tctgcgcgga cggcttttga acaacaccgc
     1801 ccagccgttc acgacggcga tgggcgatgt cgaaagcccc ggcgtgctgg ccggaatcgg
     1861 cgtgagcgcg acggtgcgcg gcttcgacct cggcatccgg tatcgcggaa ccttcggctc
     1921 gcacagccag aagtcgcaat cggcgctgct ctcggtcagc aagaagtttt agttccgcgt
     1981 acctttttcg agccaatcct gcctgccgcg ctctcacaaa agggcgcggc tttttttatg
     2041 cgcgcaggtc gagcatccgg ccgggcgccg tcagttgcgc ggtgggggcg gtcgccggat
     2101 cgagcgccgc tttctgcgtg ggatcggaag cggcgtcgac gccatagggg ccggatctgg
     2161 ggtgcgccgg gtcgatgtgc tttttcgcat agtcgacatc cgtgcagttt ttgcacagga
     2221 agccgttgac ctgcaccgga tggggatagt cgccgctgat cgccatgttc gcccgccttt
     2281 tgcgccgtac cccatctgtt acggcggccg ggcgcaaagg ttacagcagc gaggcggaaa
     2341 tattgatcat cagggccagc acggtggcgt tgaacaggta ggcgatcacg cagtggatca
     2401 ggccgatgcg gcgcatggcg gtgctggcga agcagacgtc ggcggtctgg ccggaggtgc
     2461 cgatgataaa ggcga
//
LOCUS       DASVLL010000689         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_102798,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000689 DASVLL010000000
VERSION     DASVLL010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102798"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /gene="msrA"
                     /locus_tag="VF402_11315"
     CDS             complement(<1..362)
                     /gene="msrA"
                     /locus_tag="VF402_11315"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7800933.1"
                     /translation="MLKKTIFAAAAAIALSATAFAASAADQVAVFAGGCFWSMQKAFD
                     HVPGVVKTRAGFMGGHVKNPTYEQVVTETTGHVESVEVTYDPARVSYDQLLQTFWRHT
                     DPTDPNGVICDFGPSYRTA"
     gene            complement(371..841)
                     /gene="msrB"
                     /locus_tag="VF402_11320"
     CDS             complement(371..841)
                     /gene="msrB"
                     /locus_tag="VF402_11320"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7800934.1"
                     /translation="MLRRDFLSLVGIGALGAALAGRAGAEAITPLNLSDAEWKKRLPP
                     LSYAVLRHEATERPFTSPLLNEHRKGVFACLGCDLPLFNSDTKFDSGTGWPSFFRAIP
                     NHLKTKADFAIGAPRTEYHCARCGGHQGHVFDDGPPPTGKRYCNDGAALQFHPA"
     gene            1072..2103
                     /locus_tag="VF402_11325"
     CDS             1072..2103
                     /locus_tag="VF402_11325"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7800935.1"
                     /translation="MKKSFVFGAATAAVLLAAAAGANAQSWADSTTVSGRIYADVTDL
                     SVKTNDVKQASSGFGTDVKRFYVGIDHKFNDIWFVNVTTDFTTVNTNSGNLDSVYIKK
                     AYIEGKFSPALDLRVGSTDLPWIPYAEGVYGFRYIENTLSDKAKVGTSADWGVHASGK
                     LGPIFSYAISAVNGNGYKNTARAKGLDVEGRLSAKLDQWDFAIGGYTGKLGATYGVAT
                     PNTASRFNALAAWTGDRAKVGVEYFSANDYSAAIVKGAPEDKADGVSVFGAYRLNPVY
                     TLIGRYDDVKPNKDTNTSKEDKYYNVGVQYAAFKNVNFALMLKHDELSSTGVKTVSDE
                     IGVWSELRY"
     gene            2472..3527
                     /gene="pstS"
                     /locus_tag="VF402_11330"
     CDS             2472..3527
                     /gene="pstS"
                     /locus_tag="VF402_11330"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX7800936.1"
                     /translation="MFKFLTAAAAVALLIAPVASTAFAADTVSGAGSTFAAPLYAKWA
                     EGYKRATGASLNYQAIGSGGGIKQIEAGTVDFGGTDKPLTAEVLEQNGLYQFPTAVGG
                     VVPAINLPGVPSGKLKLPGQVLADIFQGKIAKWNDPAIAKWNAGLTLPNLPITVVHRS
                     DGSGTTFIFTTYLSIASADWKSGVGANDSVEWPVGVGGKGNDGVAALVRGTAGSIGYV
                     EYAYVMKTGIAYALVASHDATWPLPTAAAFKAATAGADWAHAPGNYLMIVNQPGANAW
                     PISGATFALVHKNPADAAQTAGVLKFFDYGFKNGDFNANDLFYVPLPASVKTLIRQQW
                     AAQVKSGGKPVYISPNK"
     gene            3715..4689
                     /gene="pstC"
                     /locus_tag="VF402_11335"
     CDS             3715..4689
                     /gene="pstC"
                     /locus_tag="VF402_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7800937.1"
                     /translation="MTSDPSASEPPALKAAAPLRPRLDVTDPIFRWVSFGAATLLLVV
                     LAGIIGSLVVGGWPALSKFGIGFFIDSSWNPVTERYGAAGPIIGTVISSALALVFALP
                     VAIGIAVYLTQFCPRALAQPLSTAVELLAGIPSIVYGMWGLFVFAPFFANHVQLPLMM
                     AAKPGSLLDKLTAGVPNGANVFTASIILAIMILPYMAAVFRELFRSIPPQVREAAFGL
                     GATPFEVVTSVIIPYVQKGMIGVIMLGLGRALGETMAVTFIIGNSHRFPKSLFDSSAT
                     LASTIANEFAEATTDMHTSALVALGLVLFLITFAVLAAARALLSSQRY"
BASE COUNT          776 a         1648 c         1573 g          868 t
ORIGIN      
        1 gcggtgcggt agctggggcc gaagtcgcag atcacgccgt tgggatcggt cgggtcggtg
       61 tgacgccaga aggtctggag caactgatcg tagctgaccc tggccgggtc gtaggtgacc
      121 tcgaccgatt cgacgtggcc ggtggtctcg gtcaccacct gctcgtaggt cgggttcttc
      181 acatggccgc ccatgaagcc cgcgcgggtc ttgaccacgc ccggcacgtg gtcgaacgcc
      241 ttttgcatgc tccagaagca gccgcccgcg aacacggcga cctgatcggc ggcgctggcg
      301 gcgaaggccg tggcgctgag ggcgatggcc gcggcggcgg cgaagatggt ctttttcaac
      361 atgacgaacc tcacgccgga tggaattgca aagccgcgcc gtcgttgcag tagcgcttgc
      421 cggtcggcgg cgggccgtcg tcgaacacat gcccctgatg gccgccgcag cgcgcgcagt
      481 gatattcggt gcgcggcgcg ccgatggcga agtcggcctt ggtcttcagg tggttcggga
      541 tggcgcggaa gaagctgggc cagccggtgc cggaatcgaa cttcgtatcg gaattgaaca
      601 gcggcaggtc gcagccgagg caggcgaaca cgcccttgcg atgctcgttc agcagcgggc
      661 tggtgaaggg ccgttcggtc gcctcgtggc gcagcacggc gtaggacagc ggcggcagac
      721 gcttcttcca ttcggcgtcg ctcaggttca gcggcgtgat ggcttcggct ccggcgcgcc
      781 cggccagcgc ggcgccgagc gcgcctattc cgaccaggct gagaaaatcg cggcgtaaca
      841 tggatggctc cttttgcggc tccgttatca tgatacgccc gtcgggccat tcggttacac
      901 tgtgactttt ttcacgcaga acgcccgccg ggccggcgct gtcatggaag tgtcgccgaa
      961 cctacacgca agcgtcaccg cccgatccta tagcgccggt ccgtggtctt cgctgttcgg
     1021 ggaccggagt ttctccgccc ggaagtcttc cggcgttcaa agggtctgtc catgaaaaaa
     1081 tccttcgtct tcggcgcggc gaccgccgcc gtcctgctgg ccgccgccgc cggcgccaac
     1141 gcccagagct gggccgactc caccacggtt tcgggccgca tctacgccga cgtcaccgac
     1201 ctgtcggtca agaccaacga cgtcaagcag gccagcagcg gcttcggcac cgacgtcaag
     1261 cgcttctatg tcggcatcga ccacaagttc aacgacatct ggttcgtcaa cgtcaccacc
     1321 gacttcacca cggtcaacac caacagcggc aatctcgatt cggtctatat caagaaggcc
     1381 tatatcgagg gcaagttctc gcccgcgctc gacctgcgcg tcggctcgac cgacctgccg
     1441 tggattccgt acgccgaagg cgtttacggt ttccgctaca tcgaaaacac cctgtcggac
     1501 aaggccaagg tcggcacgtc ggcggactgg ggcgtgcatg cctcgggcaa gctggggccg
     1561 atcttctcct acgcgatctc ggcggtgaac ggcaacggct acaagaacac cgcgcgcgcc
     1621 aaggggctgg acgtcgaggg ccgcctgagc gccaagctgg atcaatggga cttcgccatc
     1681 ggcggctata ccggcaagct gggcgcgacc tacggcgtcg ccacgccgaa cacggcgtcg
     1741 cgcttcaacg ccctggccgc ctggaccggc gaccgcgcca aggtcggcgt cgaatacttc
     1801 tcggccaacg actattcggc ggccatcgtc aagggcgcgc cggaggacaa ggcggacggc
     1861 gtctcggtgt tcggcgccta tcgcctgaac ccggtctata cgctgatcgg ccgctatgac
     1921 gacgtgaagc cgaacaagga cacgaacacc tcgaaggaag acaagtatta taacgtcggc
     1981 gtgcagtatg cggcgttcaa gaacgtcaac ttcgcgctga tgctcaagca cgacgaactg
     2041 tcctctacgg gggtgaagac cgtgagcgac gaaatcggcg tctggtcgga actgcgttat
     2101 tgagctgagg gcgccgacct ttcccctctc cccgcttgcg gggagaggac gaggagcaag
     2161 gcgcagccgc agatcctcgg gtgaggggca ggcggacggc tgagcgcaat cgtcaggcgg
     2221 cccctcaccc gatcgccttc gcctgacggc tcggctctcg tcctctcccc tcgtggaggg
     2281 gagaggggga tgatatattt gaaggccgcc gcgcgggttt cgcgcggcgg ctttttttgc
     2341 gcgtcgcgtt acggcggcgg gtttcgggcg gttgtcataa actttatccc cgtgtcatat
     2401 agctgttaca aaagacgcgt aacccgctgc gcaggacata agggtccgcc aaattcctga
     2461 ggagatgggg aatgttcaag tttctgaccg ccgcggccgc cgtcgcgttg ctgatcgctc
     2521 cggtcgcttc gacggctttc gccgccgaca ccgtttcggg cgccggttcg accttcgccg
     2581 cgccgctcta cgccaagtgg gccgaaggct ataagcgcgc cacgggcgcc agcctcaact
     2641 atcaggccat cggctccggc ggcggcatca agcagatcga ggccggcacg gtcgatttcg
     2701 gcggcaccga caagccgctg acggcggagg ttctggagca gaacggcctc tatcagttcc
     2761 cgaccgccgt gggcggcgtg gtgccggcca tcaacctgcc gggcgtgccc tcgggcaagc
     2821 tgaagctgcc gggtcaggtg ctggccgaca tctttcaggg caagatcgcc aagtggaacg
     2881 atccggccat cgccaagtgg aacgcgggcc tgaccctgcc gaacctgccg atcaccgtcg
     2941 tccaccgttc ggacgggtcg ggcacgacct tcatcttcac cacctacctg tcgatcgcct
     3001 cggcggactg gaagtcgggc gtcggcgcca acgattcggt cgaatggccg gtcggcgtcg
     3061 gcggcaaggg caatgacggc gtcgcggcgc tggtgcgcgg cacggcgggc tcgatcggct
     3121 atgtcgaata cgcctacgtc atgaagaccg gcatcgccta cgcgctggtc gcctcgcacg
     3181 acgccacctg gccgctgccg accgccgccg ccttcaaggc cgcgacggcg ggcgccgact
     3241 gggcgcacgc gccgggcaat tacctgatga tcgtcaacca gcccggcgcc aacgcctggc
     3301 cgatctccgg cgcgaccttc gcgctggtgc acaagaaccc ggccgacgcc gcccagacgg
     3361 cgggcgtgct gaagttcttc gactacggct tcaagaacgg cgacttcaac gccaacgacc
     3421 tgttctacgt gccgctgccc gcctcggtga agaccctgat ccgccagcaa tgggcggcgc
     3481 aggtcaagtc cggcggcaag ccggtctata tctcgccgaa caagtaaggc cgccgcgata
     3541 cccgccatgc gcggacaagg ggcggggccg ggcgttccgg tctcgccccg gcttcgtccc
     3601 ggactcgccc cggcttcgtc catgcccgcc cccgcctttg cttggcgccc ttgtttcgcg
     3661 ccgacccgac cggtataggc aggcgcaggg aaccttcaac cggaaccgtc atccatgaca
     3721 tctgacccgt ccgcttccga gccccccgcc ttgaaggccg ccgcgccgct caggccgcgc
     3781 ctcgacgtca cggacccgat cttccgctgg gtgtccttcg gcgccgccac cctgctgctg
     3841 gtggtgctgg ccggaatcat cggctcgctg gtcgtcggcg gctggcccgc cctgtcgaaa
     3901 ttcgggatcg gcttcttcat cgattcgtcg tggaacccgg tgaccgagcg ctacggcgcc
     3961 gccggtccga tcatcggcac cgtcatctcg tcggcgctgg cgctggtctt cgccctgccg
     4021 gtggcgatcg gcatcgcggt ctatctgacg cagttctgcc cgcgcgccct ggcccagccg
     4081 ctgtcgacgg cggtcgaact gctggccggc atcccgtcca tcgtttacgg catgtggggc
     4141 ctgttcgtgt tcgcgccctt cttcgccaac catgtgcaac tgccgctgat gatggcggcc
     4201 aagccgggat cgctgctcga caagctgacc gccggggtgc ccaacggcgc caacgtcttc
     4261 accgcctcga tcatcctggc catcatgatc ctgccttaca tggccgccgt gttccgcgag
     4321 ctgttccgct cgatcccgcc gcaggtgcgc gaggccgcct tcggtctggg cgccacgccg
     4381 ttcgaggtcg tgacctcggt gatcatcccc tatgtgcaga agggcatgat cggcgtgatc
     4441 atgctgggcc tcggccgcgc gctgggcgag acgatggccg tgaccttcat catcggcaat
     4501 tcgcaccgct tccccaagtc gctgttcgac tccagcgcga ccctggcctc gaccatcgcc
     4561 aacgagttcg ccgaagcgac caccgacatg cacacctcgg cgctggtggc gctgggcctc
     4621 gtgctgttcc tcatcacctt cgcggttctg gcggcggcgc gcgcgctgct gtcgtcgcag
     4681 cgttactaag gaggtcgtca tgaaccgcac cttccggcgc aaggccgcca acatcgtctt
     4741 cgtctcgctg tgcgtgttgg ccaccctgat cgccctggcg gcgctgggcc tgatcctgtg
     4801 gtcgctgttc aggcagggcc tcggcgcgat gaatttgcag atattcacca tggacacgcc
     4861 cgcgc
//
LOCUS       DASVLL010000690         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_263812,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000690 DASVLL010000000
VERSION     DASVLL010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263812"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VF402_11340"
     CDS             complement(<1..584)
                     /locus_tag="VF402_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX7800938.1"
                     /translation="MNIEKYSEKTQKLIQGAQSLAQGASHAYFMPVHVLKALVDDKDS
                     IALRLITLAGGRADAFAASVNLALGKLPSVSGDTQLYMNNDTARLFNAAETDAQKAGD
                     AFVTVDRLFLAALDTPEGQAALKAAGVTLKQVQAAQAEFRKGKPADSATAENGFEALK
                     KYARDLTEAAEEGKIDPVIGRDEEIRRTIQVLARR"
     gene            772..1038
                     /locus_tag="VF402_11345"
     CDS             772..1038
                     /locus_tag="VF402_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800939.1"
                     /translation="MDAKIFFPAAAIVALLLIVLSLVWPQGLGTRSPAPFGHAIEMPD
                     YYRMARERDQRRQHEAEQKAAQARADAAASAAAASASASAAALK"
     gene            1035..1871
                     /locus_tag="VF402_11350"
     CDS             1035..1871
                     /locus_tag="VF402_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7800940.1"
                     /translation="MSRTAFIFGYGFIGQAFAAAARQAGCVLRATARDADKRARLAAQ
                     DVEAVDPGDVAALRRACEADLILITPAPTDDGCPAFAAIGDWANGPWIGYLSTTGVYG
                     DLNGGWAFEETPLRPTAPEGARRVRAEAQWLSRGAHVFRLPGLYGPGRNVIERLRDAS
                     ARRIHKPGHVFSRLHHDDCAAALIASTARPRPGGIYNLCDDEPAAADAVLDYAAALTG
                     LPSPPVIPWDDPALSPGARRFYRDNRRVANARAKAELGWRPAYPTYREGLRAIFENLT
                     PR"
     gene            1956..2780
                     /locus_tag="VF402_11355"
     CDS             1956..2780
                     /locus_tag="VF402_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX7800941.1"
                     /translation="MNSINTNISAMIALQNLNAINAQLAVTQNRISTGLRVASARDDP
                     ATWSIAQNERAQVSSLDAVKTSLSRTSSSIDVAMTAGETVSDLLTQLRDKALEASDTS
                     LDSTARAALNSDFVALRNQIAQTVRLADFNGVNLLDGSQASTSALADASGVNHMTVAA
                     HNMSLGGSIVTLSAGASFASATAASHLLSSLDASIRNVSASLSSFGTASKALATHQDF
                     IGKLQDTLTGAIGDLVDADMARESANLQALQVKQQLAIQVLSIANASNNYLLKLFQ"
     gene            2849..>3049
                     /locus_tag="VF402_11360"
     CDS             2849..>3049
                     /locus_tag="VF402_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7800942.1"
                     /translation="MPASHTFLITGASSGIGAEFARQYAALGRDLILVARRLDRLEAL
                     AAELGERHGVTARCIAADLSKRE"
BASE COUNT          473 a         1080 c          984 g          512 t
ORIGIN      
        1 cggcgggcca gcacctgaat ggtgcggcgg atttcctcgt cgcgcccgat caccgggtcg
       61 atcttgcctt cctccgccgc ctcggtcagg tcgcgcgcat acttcttcag cgcctcgaag
      121 ccgttttcgg cggtggcgga atcggcgggc ttgcccttgc ggaactcggc ttgcgcggcc
      181 tgcacctgtt tcagcgtcac gcccgcggct ttcagcgcgg cctggccttc cggcgtgtcg
      241 agcgcggcca ggaacaggcg gtccacggtc acgaaggcgt cgcccgcctt ctgcgcgtcc
      301 gtctcggcgg cgttgaacag cctggccgtg tcgttgttca tgtaaagctg cgtgtcgccg
      361 ctcacgctgg gcagcttgcc caacgccaga ttgaccgagg cggcgaaggc gtcggcgcgc
      421 ccgcccgcca gcgtgatcag ccgcagcgcg atggaatcct tgtcgtcgac cagcgccttc
      481 agcacatgca ccggcatgaa ataggcgtgg ctggcccctt gcgcgaggct ctgggcgccc
      541 tgaatcagct tctgggtctt ctcggaatat ttttcgatat tcatggtggt tttggtcccc
      601 ttcaaaggcg gaaaattccg gcgcgccctt catcgaagcg acccgccagt cgtcccgata
      661 tggtgtgaca tttgcaccac acaaggtcca cgaggcaaaa taacgcacgg cccggcgcgc
      721 ccggcaaaag caggctggcg cgcgccgcgc gaaccgctta taagaccgcg catggacgcc
      781 aaaatcttct tccccgccgc cgccatcgtc gcgctgctgc tgatcgtgct gagcctcgtc
      841 tggccgcagg gtctggggac gcgctcgccg gcgcccttcg gtcacgccat cgagatgccg
      901 gactattacc gcatggcgcg cgaacgcgac cagcgccgcc agcacgaggc cgaacagaag
      961 gccgctcagg cccgcgcgga cgccgccgcc agcgccgccg ccgcttcggc ctcggcctcc
     1021 gccgccgcgc tgaagtgagc cgtacggcgt ttattttcgg ctacggcttt atcggccagg
     1081 ccttcgccgc cgccgcgcgg caggcgggct gtgtcctccg cgccaccgcc cgcgacgcag
     1141 acaaacgcgc ccgcctcgcc gcgcaggatg tcgaagccgt cgatcccggc gacgtcgccg
     1201 cgctgcggcg cgcctgcgag gccgatctga tcctgatcac gcccgcgccg accgacgacg
     1261 gctgcccggc cttcgcggcg atcggggatt gggccaacgg gccatggatc ggttatctgt
     1321 ccaccaccgg cgtctatggc gacctgaacg gcggctgggc gttcgaggag acgccgctcc
     1381 gccccaccgc gccggaaggg gcgcggcgcg tcagggccga ggcgcaatgg ctgtccagag
     1441 gcgcgcacgt ctttcgcctg ccgggccttt acgggccggg ccgcaacgtc atcgaacggc
     1501 tgcgcgacgc cagcgcgcgg cgcatccata agcccggcca cgtcttttcc cgcctgcatc
     1561 acgacgattg cgccgcggcc ttgatcgcct cgacggcgcg gccgcggccc ggcgggatct
     1621 acaacctctg cgacgacgaa ccggcggcgg cggacgcggt gctggactac gccgccgcgc
     1681 tgaccggcct gccctcgccg ccggtgatcc cgtgggacga tccggccctg tcgcccggcg
     1741 cgcggcgctt ctaccgcgac aaccggcgcg tcgccaacgc ccgcgccaag gccgagctgg
     1801 gctggcgccc cgcctatccc acctatcgcg agggcctgcg cgcaatcttt gaaaatttaa
     1861 ccccgcgttg agattttctt agttgtgttt atgcgaatgt gacattcggc aaccctcagt
     1921 agaagactgt gggcttcgta ttggtgagcg ggaagatgaa ctccatcaat accaatatca
     1981 gcgcgatgat cgcgctgcaa aacctgaacg ccatcaacgc tcagttggcg gtgacgcaga
     2041 accgcatctc gaccggcctc agggtcgcca gcgccaggga cgatccggcg acgtggtcga
     2101 tcgcgcaaaa cgagcgcgcg caggtgtcgt cgctggacgc ggtcaagacc tccctgtcgc
     2161 ggacctcatc gagcatcgac gtcgccatga cggcgggcga gaccgtctcc gaccttttga
     2221 cccagttgcg cgacaaggcg ctggaggcca gcgacacgtc gctggattcg accgcccgcg
     2281 ccgcgctgaa cagcgatttc gtcgccctgc gcaaccagat cgcccagacc gtccggctgg
     2341 cggacttcaa cggcgtcaac ctgctggacg gctcgcaggc gtcgacatcg gccctggccg
     2401 acgccagcgg cgtcaaccac atgaccgtgg ccgcgcataa catgtcgctg ggcggatcga
     2461 tcgtcaccct gtcggcgggc gcctccttcg cctcggcgac ggcggcgtcc catctgctca
     2521 gcagcctcga cgcgtcgatc cgcaacgtgt ccgcgtcgct gtccagcttc ggcacggcgt
     2581 cgaaggcgct ggccacgcat caggacttta tcggcaagtt gcaggacacg ctcaccggcg
     2641 ccatcggcga tctggtcgac gccgacatgg cgagggagag cgccaacctg caagccttgc
     2701 aggtcaagca gcaactggcg attcaggttc tgtccatcgc caacgcgtcg aacaactatc
     2761 tgctgaaact ctttcagtag cggcggattt ggcattgccg gagcggtaaa aaagtctagg
     2821 ctgcggccac gggcttcacg agttttccat gccggcttcc cacacctttc tgatcaccgg
     2881 cgcgtcgtcc ggcatcggcg cggagttcgc ccggcagtat gcggccctgg gccgcgacct
     2941 gatcctggtc gcgcggcgtc tcgaccggct ggaggcgctg gcggcggagc ttggcgaacg
     3001 gcatggcgtt acggcgcgct gcatcgccgc cgacctgtcg aagcgcgag
//
LOCUS       DASVLL010000691         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_534082,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000691 DASVLL010000000
VERSION     DASVLL010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534082"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF402_11365"
     CDS             complement(<1..345)
                     /locus_tag="VF402_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800943.1"
                     /translation="MQSKHALRLALMSGLAGLALAFAAHAQDASSPVGPDKEHMTNGR
                     EVDLLKDQIKGYNSITQNTTIKPTVYANPGDRFVEISAADQVKSMGAGVNIAGGYDAG
                     YWDGKGKAEFSDD"
     gene            complement(436..1413)
                     /gene="lpxK"
                     /locus_tag="VF402_11370"
     CDS             complement(436..1413)
                     /gene="lpxK"
                     /locus_tag="VF402_11370"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX7800944.1"
                     /translation="MIRTPAWWYRRDARGAPWWRPLLWPLSLLWRAVNAYKRATAKPY
                     RSGLRVISVGNLTLGGSGKTPIVAELLRLLGPRAVGLSRGHGGALPGPLIVDPARHGA
                     AEVGDEALLIAREHDMFVARNRAAGLRAIEATDTDIVVLDDAHQNVRIAADVRILVVD
                     GDTRDGNWPFGDEGVCPYGPMREPFHVGFARADLVVIWMPDAVTPPDPALLDKLSGKP
                     VFIARLTAEPGAGRVLGFAAIAKPWKFEATLRACGYEVAGFESFPDHAPFTEAQLADL
                     ARRAEAQLSELITTEKDWVRLTSEWRARVRCLPIRARFDTPDALLAHLA"
     gene            complement(1410..>2540)
                     /locus_tag="VF402_11375"
     CDS             complement(1410..>2540)
                     /locus_tag="VF402_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7800945.1"
                     /translation="GPLVWFHGVSVGESLSALPAVEAIRARRPDIHVLITSATTTSAE
                     ILSRRLPENAVHQFAPVDTPQAVRAFLDHWRPDLAVFIESDIWPVLLGELDARRVPRL
                     LLSARITEKTAKGWRRFRAAMAALLGGYRAISAQDGASETRLKAMGPQVAARIAPRAN
                     LKTLGAPPPVDADKGAALKAMIGGRFVWLGASTHPGEDEAIAQTLEPGLLGGDLLILA
                     PRHPIRAADIRAAVEGMGLRVAQRSRNERIDDRVQVYLADTLGELGTLFTLSDIVVMG
                     GGFLTGVGGHNPLEPARLGKSVVTGPDIANWRGVYDDLIHDGAAQMVAAEALGPLLRR
                     LRHHPDEVYAMDQRALAFSRREDGALETVMAILTPLLPEPRR"
BASE COUNT          374 a          880 c          892 g          394 t
ORIGIN      
        1 gtcgtcgctg aactcggcct tgcccttgcc gtcccagtaa cccgcgtcgt agccgcccgc
       61 gatgttgacg cccgcgccca tgctcttgac ctgatcggcg gcggagatct cgacgaacct
      121 gtcgcccgga ttggcataga cggtcggttt gatcgtggtg ttctgagtga tgctgttata
      181 gcccttgatc tggtctttca gaagatcgac ttcgcggccg ttggtcatat gctccttgtc
      241 ggggccgacg ggactggagg cgtcctgcgc gtgggccgcg aaggcgagcg ccagcccggc
      301 cagaccggac atcagcgcaa ggcgtaaggc gtgtttcgat tgcatgtgac ttcactccct
      361 ttgtgccgga cccgcctttg gcgtcgtccg atttattatt ttcattttta aaactagcgg
      421 cggcgcggcg ctgaatcaag ccagatgcgc aagcaaggcg tcaggtgtgt caaatcgggc
      481 gcgtatcggt aagcaacgga cgcgcgcgcg ccattcggac gttaagcgca cccagtcctt
      541 ttccgtggtg atgagttccg aaagctgcgc ttcggcgcgc cgcgccaggt ccgccagttg
      601 cgcctcggtg aagggcgcgt gatcggggaa gctctcgaac ccggcgacct catagccgca
      661 ggcgcgtaag gtggcctcga acttccacgg cttggcgatg gcggcgaagc cgaggacgcg
      721 gcccgcgccc ggttcggcgg tcaggcgcgc gatgaagacc ggcttgcccg acagcttatc
      781 cagcagggcc ggatcgggcg gcgtgaccgc gtccggcatc cagatcacga cgagatcggc
      841 gcgcgcgaag ccgacgtgaa acggctcgcg catcgggccg tagggacaga cgccttcgtc
      901 gccgaacggc cagttcccgt cgcgcgtgtc gccgtccacg accaggatgc gcacgtccgc
      961 ggcgatccgc acattctgat gcgcgtcgtc cagcacgacg atatcggtgt cggtcgcctc
     1021 gatggcgcgc aggcccgccg cgcggttacg ggcgacgaac atgtcgtgtt cccgcgcgat
     1081 tagcagcgcc tcgtcaccga cctcggcggc gccatgccgc gccggatcga cgatgagcgg
     1141 acccggtagg gcgccgccgt gcccgcgcga cagcccgacc gcgcgcggcc ccagcaggcg
     1201 cagcaactcc gcgacgatcg gcgtcttgcc cgacccgccc agggtcaggt tgccgacgct
     1261 gatcacccgc aaaccggagc gatagggctt cgccgtcgcg cgcttgtacg cattgaccgc
     1321 gcgccacagc agcgacagcg gccacagcag cggccgccac cacggcgcgc cgcgcgcgtc
     1381 ccggcgatac caccatgcgg gcgtgcggat catcggcgcg gctccggcag cagaggcgtc
     1441 aggatcgcca tcaccgtctc cagcgcgccg tcctcgcggc ggctgaaggc gagggcgcgc
     1501 tgatccatcg catagacctc gtcgggatga tggcgcaggc ggcgcagcag cggcccgagc
     1561 gcctcggccg cgaccatctg cgccgcgccg tcgtggatca ggtcgtcata gacgccgcgc
     1621 cagttggcga tgtccggtcc ggtgaccacg ctcttgccga gccgcgcggg ctccagcgga
     1681 ttatgcccgc cgacgccggt caggaagccg ccgcccatga ccacgatgtc ggacagggtg
     1741 aacagcgtgc ccagttcgcc cagcgtgtcg gccagataga cctgaacgcg gtcgtcgatg
     1801 cgttcgttcc tcgagcgctg cgcgacgcgc aggcccatgc cctcgacggc ggcgcggatg
     1861 tcggcggcgc ggatcggatg gcgcggcgcc aggatcagca ggtcgccgcc cagcaggccc
     1921 ggctccaggg tctgggcgat cgcctcgtcc tcgcccggat gggtgctggc gcccagccag
     1981 acgaagcgcc cgccgatcat ggccttgagc gcggcgccct tgtcggcgtc gacgggcggc
     2041 ggcgcgccca gcgtcttgag attggcgcgc ggcgcgatgc gcgcggcgac ctgcggcccc
     2101 atcgccttga gccgggtttc gctggcgccg tcctgcgcgc tgatcgcgcg gtagccgccg
     2161 agcagggccg ccatcgcggc gcggaaccgc cgccagccct tcgcggtctt ttcggtgatg
     2221 cgcgcgctca gcagcaggcg cggcacgcgg cgcgcgtcca gttcgcccag cagcaccggc
     2281 cagatgtcgc tttcgatgaa gacggccagg tccggccgcc aatgatcgag gaaggcgcgc
     2341 acggcctgcg gcgtgtcgac cggcgcgaac tgatggaccg cgttttccgg caggcgccgc
     2401 gacaggattt ccgccgaggt ggtggtggcg ctggtgatca gcacatggat gtcgggccgg
     2461 cgcgcgcgga tcgcctcgac cgcgggcagg gccgacaggc tttcgccgac gctgacgccg
     2521 tggaaccaga ccagcggccc
//
LOCUS       DASVLL010000692         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_102967,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000692 DASVLL010000000
VERSION     DASVLL010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102967"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1090
                     /locus_tag="VF402_11380"
     CDS             206..1090
                     /locus_tag="VF402_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX7800946.1"
                     /translation="MNTHAPQPWQAVHRPDAAVAATTPEAPCALVYSLPHSGRIYPES
                     FIAASRLSPEALRGSEDAWVDELVAPAAQGVHAVTAHYARAFCDVNRHPLELDARLIR
                     GELPRSALSLSARVKAGYGVIARRLSADQDIYRQPLDMDEVNLRLDLIHRPYHVALLN
                     ALLRAREAGRARGRAAILIDWHSMPGAALSYLGEAPRPDIVLGDRHGASCSPGLTRHV
                     KRFFEARGLRVGVNRPFAGGYIVEHYGRPAAGMEALQIEIHRAIYMDEASLRLHEGAA
                     RLKAAFAALTDDLRDFRG"
     gene            complement(1322..>1502)
                     /locus_tag="VF402_11385"
     CDS             complement(1322..>1502)
                     /locus_tag="VF402_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800947.1"
                     /translation="ASFGAVALLGWRQSRSRAAKGLRRLRELLEAHPYPTPTRPIQTG
                     GDMGGETGSDNALQN"
BASE COUNT          249 a          477 c          528 g          248 t
ORIGIN      
        1 tcgtagcccg cgcgttgcag cgcgcgcgac aggaagctgc gcacggaatc ctcgtcttcg
       61 gcgagcagga tcttcttcat cggatcggac atggatggcc ccggaaaatt atagtttatg
      121 cctgcggcga ggtttgtcat catgtttgcc gcgggaacgt aaattagggg tgaagacgtg
      181 gcaggaatgt cctactaaag ttctaatgaa tacgcatgct ccacagccct ggcaggccgt
      241 ccaccggccg gacgccgccg tcgccgcgac gacgccggag gcgccgtgcg cgctggtgta
      301 cagcctgccg cattcggggc gcatctaccc ggaaagcttc atcgccgcct cgcgcctgtc
      361 gccggaggcc ctgcgcggca gcgaggatgc gtgggtggac gagctggtcg ccccggcggc
      421 gcagggcgtg catgcggtga cggcgcatta cgcgcgcgcc ttctgcgacg tgaaccggca
      481 tccgctggag ctggacgcgc ggctgatccg gggcgagctg ccgcggtcgg ccctgtcgct
      541 gagcgcgcgc gtcaaggccg gttacggcgt gatcgcgcgc cgcctgagcg ccgatcagga
      601 catttaccgc cagccgctcg acatggacga ggtgaacctg cgcctggacc tgatccaccg
      661 cccctatcac gtggcgctgc tgaacgcgct gctgcgcgcg cgggaggcgg gccgggcgcg
      721 ggggcgggcg gcgatcctga tcgactggca ctccatgccg ggggcggcgc tcagctatct
      781 gggcgaggcg ccgcggcccg acatcgtgct gggcgaccgc cacggcgcca gttgctcgcc
      841 cggcctgacg cggcatgtga agcggttttt cgaggcgcgg ggcctgcgcg tcggcgtcaa
      901 caggccgttc gcgggcggct atatcgtcga gcattacggc cgtcccgccg ccggcatgga
      961 ggcgttgcag atcgagattc accgcgccat ctatatggac gaggcgtcct tgcgtctgca
     1021 cgaaggcgcg gcgcggctga aggcggcgtt cgcggcgctg accgacgacc tgcgggattt
     1081 ccgcggctga ccggctcccc cggcgcgggg caaaaaaaag ccgcgcatct cggcgcggct
     1141 caagtttaca gggaggaaac gcccaggaag ggcatcgacg ccaaagcgtc gaacaggttg
     1201 aaattacgct gcggcgcaaa ataactcaag cgcaaattgt cgcagttacg atcacgtatc
     1261 gttacaatta cgctgaattt aagggtttgg gcgtgaaaag tacgatcaag aaccgctgtt
     1321 ctcaattttg cagcgcatta tcgctgcccg tttcgccgcc catatcgccg ccggtctgga
     1381 tcgggcgcgt cggcgtcgga taggggtgcg cctccaaaag ttcgcgcagg cggcgcagcc
     1441 ccttggcggc gcgcgagcgc gactggcgcc agcccagcag ggcgacggcg ccgaagctgg
     1501 cc
//
LOCUS       DASVLL010000693         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_159781,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000693 DASVLL010000000
VERSION     DASVLL010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159781"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..527
                     /locus_tag="VF402_11390"
     CDS             108..527
                     /locus_tag="VF402_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7800948.1"
                     /translation="MLTNLSPHLAFNGDCAEAFDAYVKILGAEITFVMTMGESPMAAE
                     VPVETHGAIMHATLKLPNGALIMGADAPPHMYKAPASTSLSLSYSDVEEGRRVFRALV
                     EGGHEIMAFGETFWSRGFGMCVDRFGQHWMINCGDAM"
     gene            524..1027
                     /locus_tag="VF402_11395"
     CDS             524..1027
                     /locus_tag="VF402_11395"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7800949.1"
                     /translation="MTEAREFSVSRRLEAPPERVWRAFTDADALAAWWGPHGVSATAR
                     LDVREGGAFEIVMRGADGVAYPIEGSYLSVEAPRLLVMEMSLENHPANWRDYLAELFT
                     RAGGAPEELAQVRVVTRVTLTPEAGGTRLDIAQVYESEALRDAFAGMGNAEGWAQSLD
                     KLQRALA"
     gene            complement(1129..1710)
                     /locus_tag="VF402_11400"
     CDS             complement(1129..1710)
                     /locus_tag="VF402_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7800950.1"
                     /translation="MRRLCVFCGSSDGDDPAYLAAARELGGTLARAGIGLVYGGARVG
                     LMGAVADAALAAGGEVIGVIPTALVEKEVAHTGLSDLRVVASMHERKALMAELSDGFV
                     ALPGGIGTFEELFEVWTWAQLGYHAKPCALLNVNGFYDGLIAFLDGVAARRFLKHAHR
                     AMLIEARDADGLMTRLRAYAPPAVTKWIGPGEG"
     gene            complement(1712..2506)
                     /locus_tag="VF402_11405"
     CDS             complement(1712..2506)
                     /locus_tag="VF402_11405"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7800951.1"
                     /translation="MTDAAVRASPVAWLSQRFGQRWPRTYGAAALIGALLAFTGAMGT
                     GEAAMGTRLVYWLILMLGGTGIAHVTGALFDRLNLPLWPQIAAMLAVITPVIAAYAWA
                     VTEAFGGGRFDAQRWPAFILPSFVIGLSVAVLHTQINRVPAQSHAFAPDAAAAAAAEP
                     GAALRERLPFRHRRADIYALTAEDHYLRVHTSAGDALILMRLYDAIRELDGIEGSQTH
                     RSWWVAKDAVADAARGGGKAALTLKNGLCVPVSRSYVRALKQDGWL"
     gene            2603..3031
                     /locus_tag="VF402_11410"
     CDS             2603..3031
                     /locus_tag="VF402_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX7800952.1"
                     /translation="MTPIEAFSHPHLPDLTPIAQAPLVIRVHLLLALGAFAVATFQIL
                     GPKGTGLHRVLGWIWVAFMAVVAISSFFIHVINPDGFSLIHLLSVVTLIALPVMVYAA
                     RRRNVKLHKSVATRLYLFALVGAGLFTLFPGRLIWRVFFG"
     gene            3388..>3847
                     /locus_tag="VF402_11415"
     CDS             3388..>3847
                     /locus_tag="VF402_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7800953.1"
                     /translation="MNIHEHQAKAVLKAFGVAVPRGYPAFSPDEAEKAAKELAKDGSK
                     VFVVKSQIHAGGRGKGRFEGLGPDAKGGVRVVRSPEEVKANADEMLGRVLVTHQTGPH
                     GKQVNRLYIEEGADIAKELYLSLLVDRETSKVSVVASTEGGMDIEDVAHAT"
BASE COUNT          637 a         1316 c         1257 g          637 t
ORIGIN      
        1 gccgaggcgc gtattgtttc ttgatgagcg gcggccttga gtgcttactg ccataatccg
       61 gcgccgccgc attcatgctg acagggttcg ataaggagac caacgccatg ctcaccaatc
      121 tgtcgccgca tctggccttc aacggcgatt gcgccgaagc cttcgacgcc tatgtgaaga
      181 ttctcggcgc cgagatcacc ttcgtgatga cgatgggcga atcgccgatg gcggccgagg
      241 ttccggtcga aacccacggc gccatcatgc acgccacgct caaactgccc aacggcgcgc
      301 tgatcatggg cgccgacgcg ccgccgcaca tgtacaaggc cccggcgtcg acaagcctca
      361 gcctcagcta cagcgacgtc gaggaaggcc gccgcgtctt cagggcgctg gtcgagggcg
      421 ggcatgagat catggccttc ggcgaaacct tctggtccag gggcttcggc atgtgcgtcg
      481 accgcttcgg ccagcactgg atgatcaatt gcggagacgc gatgtgacgg aggcgcgcga
      541 attttcggta tcccggcgcc tggaggcgcc gccggagcgg gtctggcgcg ccttcaccga
      601 cgcggacgcg ctggccgcgt ggtgggggcc gcacggagtc tccgccacgg cccggctcga
      661 cgtgcgcgaa ggcggcgcgt tcgagatcgt gatgcgcggc gccgacggcg tggcctatcc
      721 gatcgaggga agctacctgt cggtcgaggc gccgcgcctg ctggtcatgg agatgtcgct
      781 ggagaaccac ccggccaact ggcgcgacta tctggccgaa ctgttcacca gggcgggcgg
      841 cgcgccggag gagctggcgc aggtgcgcgt ggtcacgcgc gtgacgctga cgccggaagc
      901 gggcggtacg cggctcgaca tcgcgcaggt ctatgagtcc gaagcgctgc gcgacgcctt
      961 cgcgggcatg ggcaacgccg aaggctgggc gcagtcgctc gacaagttgc aacgcgcgct
     1021 cgcctgacgc tccttgcttc ccctctcccc gtaagcgggg agaggacgag agccgagcgt
     1081 aagcgaaggc gatcgggtga ggggctgctc gccggttatg cgccgttgct aaccttcccc
     1141 cggcccgatc cacttggtca cggcgggcgg cgcataggcc cgcagccgcg tcatcagccc
     1201 gtccgcgtcg cgcgcctcga tcagcatggc gcggtgcgcg tgcttgagga agcgccgcgc
     1261 cgccacgccg tccagaaacg cgatcaggcc gtcgtaaaag ccgttgacgt tcagcagcgc
     1321 gcacggcttg gcgtggtagc cgagctgcgc ccacgtccag acctcgaaca gttcctcgaa
     1381 cgtgccgatg ccgccgggca gggcgacgaa accgtccgac agctcggcca tcagcgcctt
     1441 gcgctcatgc atcgacgcca cgacgcgcag gtcgctcagg cccgtgtgcg cgacctcttt
     1501 ttccaccagc gcggtcggga tcacgccgat gacctcgccg cccgccgcca aagccgcgtc
     1561 ggcgaccgcg cccatcagcc cgaccctggc cccgccatag acgaggccga tcccggcgcg
     1621 cgccagcgtc ccgcccagtt cgcgcgccgc cgccagatag gccggatcgt cgccgtcgct
     1681 cgatccgcaa aacacgcaca gccgtcgcat cctataacca cccgtcctgt ttcagcgccc
     1741 tgacatagct gcggctgacc ggaacgcata gcccgttttt cagcgtcagc gccgccttgc
     1801 cgccgccgcg cgccgcgtcc gcgaccgcgt ccttcgccac ccaccaggag cggtgcgtct
     1861 ggctgccctc gatcccgtcc agttcgcgga tcgcgtcgta cagccgcatc aggatcagcg
     1921 cgtcgcccgc cgaggtgtga acgcgtaagt agtggtcctc cgccgtcagc gcgtagatgt
     1981 cggcgcgacg gtgcctgaac ggcagccgct cgcgcaaggc ggcgccgggt tcggcggcgg
     2041 cggcggcagc agcgtctggc gcgaaggcgt ggctctgcgc cggaacgcgg ttgatctggg
     2101 tgtgcagcac cgccacgctg agcccgatca cgaagctggg caggatgaag gcgggccagc
     2161 gctgcgcgtc gaaccgcccg ccgccgaacg cctccgtcac ggcccacgca taggccgcga
     2221 tcaccggcgt gatgacggcc agcatggcgg cgatctgcgg ccacagcggc aggttcagcc
     2281 ggtcgaacag cgcgcccgtc acatgcgcga tccccgtgcc gcccagcatc aggatcagcc
     2341 aatagacaag gcgcgtcccc atcgccgcct cgcccgtgcc catcgcgccg gtgaaggcca
     2401 gcagcgcgcc gatcagcgcc gccgcgccgt aggtgcgcgg ccagcgctgg ccgaagcgtt
     2461 gtgaaagcca tgcaactggc gaagcgcgaa cggcggcgtc ggtcatatca cgtaaaaaca
     2521 ggggtggttc acgaaggcgg cgcggcgcga accgcgcggt tcgctcctta tgcggcatcc
     2581 gccgacaaaa aggaaccacg tcatgacgcc gatcgaagcc ttctcccatc cgcacctgcc
     2641 cgacctgacg cccatcgcgc aggcgccgct ggtcatccgc gttcacctgc tgctggccct
     2701 gggcgccttc gcggtggcga cctttcagat tctggggccg aaggggacgg gtctgcaccg
     2761 cgtgctcggc tggatctggg tcgccttcat ggccgtggtc gcgatctcca gcttcttcat
     2821 tcacgtgatc aacccggacg ggttcagcct gattcacctc ctgtccgtcg tgaccctgat
     2881 cgcgctgccg gtcatggtct atgcggcgcg caggcgtaat gtgaagctgc acaagagcgt
     2941 ggcgactcgg ctctacctgt tcgcgctggt cggggcgggc ctgttcaccc tgttccccgg
     3001 acgcctgata tggcgggtgt ttttcggctg aatttgcttt atattgtcag acatttaagc
     3061 cctttcgcgc cgctgcggcg cagcgtgaaa gggctgacaa cgctgtcctc gcggcgatta
     3121 ttgcgttagc ggtaacacgc ttgtcttgcc tgccttagcg ggacattcga ttttcgtaaa
     3181 agccgtcaaa tttttgtgag tgagcggttg cgcacaggcg accgcctctt tataaagcgc
     3241 cccgatcgca agatacgtct ttgggacgag caacggagtt cgccctgctg tcgctcgggt
     3301 ccggttcgcc gggtccaccc tttaacaaga gcagattttc gcacgcgcga agccgctctc
     3361 ccagtcttta ttcaaaagat cggtttcatg aacattcacg agcatcaagc caaggccgtc
     3421 ctgaaagcgt tcggcgtggc cgttccgcgc gggtaccccg ccttttcccc cgacgaggcc
     3481 gaaaaggccg ccaaagagct ggccaaggac ggctccaagg tcttcgtcgt caagtcgcag
     3541 atccacgccg gcgggcgcgg caagggccgc ttcgaagggc tcggccccga cgccaagggc
     3601 ggcgtgcgcg tcgtcaggtc gccggaagag gtcaaggcca acgccgatga aatgctcggc
     3661 cgcgtgctgg tgacgcacca gaccggtccg cacggcaagc aggtcaaccg cctctatatc
     3721 gaggaaggcg ccgacatcgc caaggaactc tatctgtcgc tgctcgtcga ccgcgaaacc
     3781 tccaaggtct cggtcgtcgc ctcgaccgaa ggcggcatgg acatcgagga cgtcgcccat
     3841 gcgacgc
//
LOCUS       DASVLL010000694         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_319033,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000694 DASVLL010000000
VERSION     DASVLL010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319033"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VF402_11420"
     CDS             <1..815
                     /locus_tag="VF402_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800954.1"
                     /translation="RSGRGWPSLILLATAYGLIEEGFVTQSLFNPNYLHLRLLDFGYV
                     PWLGTGLPWLIYVVSIHVVWSMSVPIGLSEVLFRNKRDRPWLGPVGIGVFALLFLAGT
                     ALIAVYTHKSLPFMASPAQFAVTGVAVAGLIAAAFALPKTAPATDRAAPHPLILFAVA
                     FIAGSTLMLLQLMAQHSLHWPWTTYVAALLLIEAAFVAFMIACTRGRRWTDLQRCALM
                     SGGLLVYGWSGFVIDRDLHGAADRPAHALIAAAFALLLGWAWRVAARPAKKS"
     gene            complement(742..>1835)
                     /locus_tag="VF402_11425"
     CDS             complement(742..>1835)
                     /locus_tag="VF402_11425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7800955.1"
                     /translation="YEIVRHDLRPAFRKLWSGRARLPGKGGVWFLHANAPECLVAFLA
                     HSPEQWADRYRIGYWAWETPRAPEEWVWLADYLHEIWVPSRFVRDALIAAFVAARRED
                     LSDRVRVMPHPVPVSPPARYDYVHQRFDMTPELCEVLCLFDVKSSAARKNPWAVLDVW
                     KRAFPAPSDTARLTLKVSDLSGDEATQRRLLRELKGRPDIRLFEERLSAADMQAFLGG
                     FDVLISLHRSEGFGLPLAEAMGSQTAVIATGWSGNLDFMTQENSRLIPARLIPLRDPD
                     GPYCRQSGRPGQVWAEPDRIAAIDALLELARSESKRRDLAYAGLRAVRALSEPWRREA
                     LDALPFRTFLPDAPPHATPSPAKARTPRR"
BASE COUNT          282 a          653 c          604 g          296 t
ORIGIN      
        1 ggcggtcggg acgcggctgg cccagcctga tcctgctggc gacggcctac gggctgatcg
       61 aggagggctt cgtcactcag tcgctgttca atccgaacta cctgcacctg cgcctgctcg
      121 atttcggcta tgtcccgtgg ctcggcacgg gcctgccatg gctgatctat gtcgtctcga
      181 tccacgtcgt ctggagcatg agcgttccga tcgggttgag cgaagtcctg ttccgcaaca
      241 agcgcgaccg gccgtggctc ggtccggtcg gcatcggcgt tttcgcgctg ctgttcctgg
      301 ccggaacggc gctgatcgcc gtctataccc ataaatccct gccgttcatg gccagtccgg
      361 cgcagttcgc cgtcaccggc gtcgcggtcg cggggctgat cgccgccgcc ttcgccctgc
      421 cgaagaccgc gcccgccacg gatcgcgcgg cgccgcatcc gctcatcctg ttcgccgtcg
      481 ccttcatcgc gggatcaacg ctcatgctgc tgcaattgat ggcgcagcat agcttgcact
      541 ggccctggac cacctatgtc gcggcgctgc tgctgatcga ggcggcgttc gtcgccttca
      601 tgatcgcctg cacgcgcgga cgcagatgga cggacctgca acgttgcgcg ttgatgagcg
      661 gcgggctgct ggtctatggc tggagcggct tcgtgatcga ccgcgacctg cacggcgcgg
      721 cggaccggcc cgcccatgcg ctcatcgccg cggcgttcgc gcttttgctg ggctgggcgt
      781 ggcgtgtggc ggcgcgtccg gcaaaaaagt cctgaacggc aaggcgtcca gcgcctcgcg
      841 ccgccacggc tcgctcaggg cgcgcaccgc gcgcagcccg gcataggcca ggtcgcggcg
      901 cttcgactcc gaccgcgcca gctccagcag ggcgtcgatg gcggcgatac ggtcgggttc
      961 ggcccagacc tggccgggcc tgccggattg gcggcaatag gggccgtcgg ggtcgcgcag
     1021 cgggatcagc cgcgccggaa tcaggcggct gttctcctgc gtcatgaagt cgagattgcc
     1081 ggaccagccg gtcgcgatca ccgccgtctg cgaccccatc gcctcggcca gcggcaggcc
     1141 gaagccttcc gaccggtgca gcgagatcag cacgtcgaag ccgccaagga aggcctgcat
     1201 gtcggcggcg ctcaggcgct cctcgaacag acggatatcg ggccgcccct tgagttcgcg
     1261 caacaggcgg cgctgcgtcg cctcatcgcc gctcaggtcc gagactttca gcgtcagccg
     1321 cgccgtgtcg gacggcgccg gaaaggcgcg cttccagacg tcgagaaccg cccacggatt
     1381 cttgcgcgcc gccgacgact tgacgtcgaa caggcacaga acctcgcaca gctccggcgt
     1441 catgtcgaaa cgctgatgca cataatcgta acgcgccggc gggctgaccg gcacgggatg
     1501 cggcatgacg cgcaccctgt cgctcaaatc ctcgcgccgc gccgccacga acgccgcgat
     1561 cagcgcgtcg cgcacgaacc ggctcggaac ccagatctca tgcagataat ccgccagcca
     1621 gacccattcc tccggcgcgc gcggcgtctc ccacgcccag tagccgatgc gatagcggtc
     1681 cgcccactgc tccggagaat gcgcgagaaa ggcgaccaga cattccggcg cattggcgtg
     1741 caaaaaccac acgccgccct tgccgggcag gcgcgcgcgg cccgaccaca gcttgcggaa
     1801 ggccggacgc agatcgtgac gcacgatctc gtacc
//
LOCUS       DASVLL010000695         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_371209,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000695 DASVLL010000000
VERSION     DASVLL010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371209"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2044)
                     /locus_tag="VF402_11430"
     CDS             complement(<1..2044)
                     /locus_tag="VF402_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX7800956.1"
                     /translation="MTSSLALRRLFLTTSFLALAAPALAHADDAPGKPDKAPEEITVI
                     GKRVIKGSAGATGLDLSLRQTPQSVTVVDEQQIEDFSLTTANQVLTAIPGIQVDAAET
                     DRSVYSARGFDITNFQVDGVGQPLIWGIQYGDIDSVIYDRVEAIRGSTGMLTGTGNPS
                     ATINYIRKRPTSTFQSELTAAYGSWNDKRLTADLSGPLNKSGTLEGRFVYANEDKDTW
                     LDYNHVNRNVYYGVLAWDITPRLKLTGGYSQQDNLADGVTWGALPLTYSDGTPITSYK
                     VSDSTSAPWTYWNTHDRTAFGELSYAFDNGWTAKITATHRSFDYTDKLLYAYGSPDTS
                     DSVNILAWPGVFPAKADQAMLDAQASGPFSLFGRAHELVFGVNAATATTRQWESYSTT
                     TSYYPALSTWGDAYPAEPRFTALDLAEDTLTKTVRVYGAAHLNFTDRLKGVVGFNAFQ
                     LKGTGYSYAVDQARSDSAVSPYLGAVYDLTKNVSAYASYSDIYNPQYQVDVDKKTLPA
                     ATGKAYEAGLKSEWFDRRLYASAALFKSEQHNLAEAAGTDPDSLQTYYAPTEARSQGY
                     ELEVAGKVTPNWTLNGGWTDLKIENANGDPSRLYSPRRTFKLSTTYQIPQWRDLKLGA
                     AVRWQDDTSTVDNGTTIVQRAYAVADLMASFRLTDHVAAALNVNNAFDQKYWSSLAW"
BASE COUNT          340 a          623 c          749 g          417 t
ORIGIN      
        1 cccaggcgag gctggaccag tatttctggt cgaaggcgtt gttcacgttc agcgcggcgg
       61 cgacatgatc ggtcaggcgg aagctggcca tcaggtcggc gacggcgtag gcgcgctgca
      121 cgatggttgt gccgttgtcc accgtcgagg tgtcgtcctg ccagcgcacc gccgcgccca
      181 gcttcaggtc gcgccattgc ggaatctgat aggtggtcga cagcttgaag gtgcggcgcg
      241 gcgaatacag gcgcgacgga tcgccgttgg cgttctcgat cttcagatcg gtccagccgc
      301 cgttcagcgt ccagttcggc gtgaccttgc ccgcgacttc cagttcgtag ccctgagagc
      361 gcgcctcggt cggcgcgtaa taggtctgga ggctgtccgg gtccgtgccg gcggcttcgg
      421 ccaggttgtg ctgctccgat ttgaacagcg ccgcggaagc gtagagccgc cggtcgaacc
      481 attccgattt caggcccgcc tcataggcct tgccggtggc ggcgggcagg gtcttcttgt
      541 cgacgtccac ctgatactgc ggattgtaga tgtcggaata gctggcatag gccgagacgt
      601 tcttcgtcag gtcatagacc gcgcccagat aggggctgac ggcgctgtcc gagcgcgcct
      661 gatcgaccgc gtaggaatag ccggttccct tgagctggaa ggcgttgaag ccgacgacgc
      721 ctttcagccg gtcggtgaaa ttcagatgcg ccgcgccata gacgcgcacc gtcttggtca
      781 gcgtgtcctc ggccagatcg agggcggtga accgaggctc cgccggatag gcgtcgcccc
      841 atgtcgaaag cgccggatag taggaggtgg tggtcgaata ggattcccat tgccgggtgg
      901 tcgcggtcgc ggcgttgacg ccgaagacca gttcgtgcgc gcgcccgaac agcgagaacg
      961 gaccgctggc ctgagcgtcg agcatggcct gatcggcctt ggccggaaag acgccgggcc
     1021 aggcgaggat gttgacgctg tcgctcgtat cggggctgcc gtaggcgtag agcagcttgt
     1081 cggtatagtc gaagctgcga tgcgtggccg tgatcttggc cgtccagccg ttgtcgaagg
     1141 cgtagctcag ctcgccgaaa gcggtcctgt cgtgggtgtt ccaataggtc cacggcgccg
     1201 aggtgctgtc cgagaccttg tagctggtga tcggcgttcc gtccgaatag gtcaggggca
     1261 gcgcccccca ggtcacgccg tcggccaggt tgtcctgctg cgaatagccg ccggtcagct
     1321 tgagtctggg cgtgatgtcc cacgccagaa cgccgtaata gacgttgcgg ttgacgtggt
     1381 tgtagtcgag ccacgtatcc ttgtcctcat tggcgtacac gaagcggccc tccagcgtgc
     1441 cgctcttgtt cagcgggccg gacagatcgg cggtcaggcg tttgtcgttc cacgaaccgt
     1501 aggcggcggt cagttcgctc tggaaggtcg aggtcgggcg cttgcggata tagttgatgg
     1561 tggccgacgg gttgccggtg ccggtcagca tgccggtcga gccgcgaatc gcctcgacgc
     1621 ggtcgtagat gacgctgtcg atgtcgccgt actggatgcc ccagatcagc ggctggccga
     1681 cgccgtcgac ctgaaaattg gtgatgtcga agccgcgcgc gctatagacg ctgcggtcgg
     1741 tttcggcggc gtcgacctgg atgcccggta tggcggtcag cacctgattg gccgtggtca
     1801 gcgaaaaatc ctcgatctgc tgttcgtcca ccacggtcac cgactgcggc gtctggcgca
     1861 aggacaggtc gaggccggtg gcgccggccg aacccttgat cacgcgcttg ccgatgacgg
     1921 tgatctcctc gggcgccttg tccggcttgc ccggagcgtc atcggcgtgc gccagcgcgg
     1981 gcgcggccag ggccaggaac gaggtcgtca ggaaaaggcg gcgaagagcg agggaggagg
     2041 tcatggcacg cttatgagaa tgatttgcaa tagcgccgcg tttacgcgct caggcgcgcc
     2101 gcgtcaactg catttgcgaa ttattctca
//
LOCUS       DASVLL010000696         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_264475,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000696 DASVLL010000000
VERSION     DASVLL010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264475"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VF402_11435"
     CDS             <1..699
                     /locus_tag="VF402_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7800957.1"
                     /translation="APHRVVICVSGGVSADDVYRAARGHIEPVAPHAGFGAPEPLAFA
                     PGRAVRRRRIEQANLTLAFKGVGRLEADIVPLRLFSEILGGGMASRLFQEAREKRGLA
                     YAVDSWATPYRDGGVFGVYAGCAPGDAAALQDLILQVMAQLARDPSADELARAKAQFQ
                     TSLFLNHENAAARAGSFASYVSVFDRVLSLDEVADDIGRVTRDDLVRIGDMLIGQGGF
                     ASACLGPKSMRLAA"
     gene            800..>1699
                     /locus_tag="VF402_11440"
     CDS             800..>1699
                     /locus_tag="VF402_11440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7800958.1"
                     /translation="MAEFGLFGVRRDFDAGAAREVVMAKAKAEGAIFDAAGLTKAAAA
                     ADSLFWAYDAPLGRFEFHGDAQKLGLPPLNGRLSPQDLALLLDIGEVDRLEAALHTLD
                     VLTAADLPGEGPAGYVQCVLRLRDQRCVYLKGRRVGRTMAMGTLTELSRDARHGGSEA
                     GLELHDIHIDMLTGLHSRHGFLNAAQAALTQPGDYDLVVGDFNRFRRLNEALGHARAD
                     RVLALLAQRLRDAFPPSAHTVLARLGEDEFAVLTQRGFPRVSERMRQALERALNVAGF
                     DIHPTFSMGAVSVEGGEAALDSGE"
BASE COUNT          240 a          536 c          634 g          289 t
ORIGIN      
        1 gcgccgcatc gcgtggtgat ctgcgtgtcg ggcggcgtca gcgccgacga cgtctatcgc
       61 gcggcgcgcg gccatatcga gccggtcgcg ccgcacgccg gtttcggcgc gcccgaaccg
      121 ctggccttcg cgccgggacg cgcggtgcgc cgccgccgca tcgagcaggc gaacctgacg
      181 ctggcgttca agggcgtcgg gcggctcgaa gcggatatcg tccccttgcg gctgttcagc
      241 gagattctgg gcggcggcat ggcgtcgcgc ctgtttcagg aggcgcgcga gaaacgcggc
      301 ctggcctatg cggtcgattc gtgggcgacg ccctatcgcg acggcggcgt gttcggcgtc
      361 tatgcgggct gcgcgcccgg cgacgcggcg gcgttgcagg atctgatcct gcaggtcatg
      421 gcgcagttgg cgcgcgaccc gtccgcagac gaactggcgc gcgccaaggc gcagttccag
      481 acctcgctgt tcctcaacca cgaaaacgcc gccgcgcgcg cgggcagctt cgccagctac
      541 gtgtcggtgt tcgatcgcgt gctgagcctc gacgaggtcg ccgacgacat cgggcgcgtg
      601 acgcgcgacg acctggtgcg catcggcgac atgctgatcg ggcagggcgg cttcgcctcg
      661 gcgtgtctcg gccccaaatc gatgcggctg gcggcctgat ttatggtgcg gcgcaaataa
      721 tttcatccga tcttatccaa aattaacctg ttaaggttaa ggccagatgg ttgcgtgacg
      781 gttggcttcg ggtaagctta tggcggaatt cggtctgttt ggggtgcggc gcgacttcga
      841 cgcgggcgcg gcgcgagagg tggtcatggc gaaggcgaaa gcggaaggcg cgatattcga
      901 cgccgccgga ctgacgaagg ccgccgccgc cgccgacagc ctgttctggg cctacgacgc
      961 gccgctgggc cggttcgagt tccacggcga cgcgcaaaag ctgggcctgc cgccgctgaa
     1021 cgggcgcctg tcgccgcagg atctggcgct tttgctggac atcggcgagg tcgaccggct
     1081 ggaagcggcc ctgcacacgc ttgacgttct caccgccgcc gacctgccgg gcgaaggacc
     1141 ggcggggtat gtgcagtgcg tgctgcggct cagggatcag cgctgcgtct atctgaaggg
     1201 gcggcgcgtc ggtcggacga tggcgatggg cacgctgacc gagctgagcc gcgatgcgcg
     1261 tcatggcggg agcgaagccg gtctggagtt gcacgacatc catatcgaca tgctgaccgg
     1321 cctgcacagc cgtcacggct tcctgaacgc ggcgcaggcc gcgctgacgc agccgggcga
     1381 ttacgatctg gtggtcggcg atttcaaccg tttccggcgg ctgaacgagg cgctgggcca
     1441 cgcgcgcgcc gaccgggtgc tggcgctgct ggcccaacgt ttgcgcgacg cctttccgcc
     1501 ttcggcgcac accgtgctgg cgcggctggg cgaggacgag ttcgcggtgc tgacgcaacg
     1561 cggttttccg cgcgtgtcgg agcgtatgcg gcaggcgctg gaacgcgcgc tcaatgtcgc
     1621 cgggttcgat atccatccaa ccttttcgat gggcgcggtg tcggtcgagg gcggcgaggc
     1681 ggcgctggac agcggcgag
//
LOCUS       DASVLL010000697         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_47066, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000697 DASVLL010000000
VERSION     DASVLL010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47066"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /gene="trpB"
                     /locus_tag="VF402_11445"
     CDS             complement(<1..1126)
                     /gene="trpB"
                     /locus_tag="VF402_11445"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7800959.1"
                     /translation="MNDSVLRSNAYDLPDAKGRFGQFGGLYVPETLMPLVLELNEAWR
                     SARNDPEFWSEYHSLMKYFVGRPSPLYFAERLTEHFGGARIYLKREELNHTGAHKINN
                     CIGQILLAKRMGRTRIIAETGAGQHGVASATVCARFDLPCVVYMGALDVERQKPNVFR
                     MRLLGTEVVPVTNGNGTLKDAMNEALRDWVTNVHDTYYMIGTAAGMHPYPEMVREFQT
                     VIGKETKQQIMELEGRLPDAVIACVGGGSNAIGMFHPFLDDEGVKIVGVEASGHGLEV
                     YNGHAASMNGGRPGVLHGNRTYLLQDEDGQILEGHSISAGLDYPGVGPEHAYLFDTGR
                     ARYVTATDKEALEAFQLTSKLEGIIPALESSHALARVGDLA"
     gene            complement(1193..1843)
                     /locus_tag="VF402_11450"
     CDS             complement(1193..1843)
                     /locus_tag="VF402_11450"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX7800960.1"
                     /translation="MQPDIKICGISTPEAARAALDHGAAYLGFIHFGKSPRHLGLDRM
                     AGLLRAIRAETPDARRVSVVVDPDDALIDALIHEVKPDLIQLHGKESPARVAAVRARA
                     GLPVIKAVSVAEAADLAAAPAYEDAADYLLFDARTPKGAALPGGMGLRFDWTIMRGWT
                     GAKPWFLAGGLDAGNVAEAVRLSGAEALDVSSGVERAPGVKDPVLISDFLRAAKSL"
     gene            2009..2956
                     /locus_tag="VF402_11455"
     CDS             2009..2956
                     /locus_tag="VF402_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7800961.1"
                     /translation="MNDYVLLSDISNGNSLKLALARPGSRPETTTYYACNDFDGFKDA
                     ISDFLNDKGRPSLAGAAISAAGWERGGVIRLPNHGFSISRGDMRDFLSIQRVNLVNDF
                     VAKALAIPRLRESERQQICGGEPMEEQVIAVLGPHNGLGMSALAPDGMGAWTAMPTEG
                     GHSDLAATNALEDQVLGVLMRKYGHVSRERVISIPGLVDIWRALAEIDGDDIDVPGPE
                     EIIARAMAEDTRAHQVIDLSMGWFAAMAADVALILGARGGVYLAGDLLDMIGDLFDVE
                     AFVKRYTDKGRLSGYVAEIPVFRATAQDMEVIGLATLFD"
     gene            2993..>3261
                     /locus_tag="VF402_11460"
     CDS             2993..>3261
                     /locus_tag="VF402_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7800962.1"
                     /translation="MTAYVLLCDFTFGSYIRLALAEAGRRPEPPSLRACADRAGFDAQ
                     VMGFLADHGHPRLIGAALSTRGWETHGVLHLPDEGMHLTRDNVRGL"
BASE COUNT          571 a         1052 c         1063 g          575 t
ORIGIN      
        1 tggccagatc gccgacgcgc gccagggcgt gcgaggattc cagcgccgga atgatgcctt
       61 ccagcttcga ggtcaactgg aacgcctcca gcgcctcctt gtccgtcgcg gtgacgtagc
      121 gcgcgcggcc ggtgtcgaac aggtaggcgt gttcggggcc gacgcccgga tagtcgaggc
      181 ccgccgagat cgagtggcct tcgagaatct ggccgtcctc gtcctgcaac agataggtgc
      241 ggttgccgtg cagcacgccg ggacgcccgc cgttcatcga ggcggcgtgg ccgttataga
      301 cctcaaggcc gtgacccgac gcctcgacgc cgacgatctt cacgccctcg tcgtcgagga
      361 acgggtggaa catgccgatg gcgttcgacc cgccgccgac gcaggcgatc accgcgtcgg
      421 gcagacggcc ttccagctcc atgatctgct gcttcgtctc cttgccgatc acggtctgga
      481 actcgcgcac catttccggg tacggatgca tgcccgccgc cgtgccgatc atgtaatagg
      541 tgtcgtggac gttggtgacc cagtcgcgca aggcttcgtt catcgcgtct ttcagcgtgc
      601 cattgccgtt cgtcaccggc acgacctccg tgcccagaag gcgcatgcgg aagacattgg
      661 gtttttgccg ctcgacgtcg agcgcgccca tatagacgac gcacggcagg tcgaagcgcg
      721 cgcacacggt cgccgaggcg acgccgtgct ggcccgcgcc ggtttccgcg atgatgcgcg
      781 tgcggcccat gcgtttggcc agcaggatct gaccgatgca gttgttgatc ttgtgcgcgc
      841 cggtgtggtt cagctcctcg cgcttgagat agatcctggc gccgccgaaa tgctcagtca
      901 gccgctcggc gaaatagagc ggcgaggggc ggccgacgaa atacttcatc aagctgtggt
      961 attcggacca gaattccgga tcgttgcgcg cgcttcgcca cgcttcgttc agctccagca
     1021 ccagcggcat cagggtctca ggcacataga gaccgccgaa ttggccgaac ctgcccttgg
     1081 cgtccggcag gtcgtaggcg ttggatcgca gaacggaatc gttcacggaa taaggctcct
     1141 ttttcaggcg cgggctttgt ttgtcattga tgacaaacaa aagagactgg cgttatagcg
     1201 attttgcggc gcgaagaaag tccgaaatca gaacggggtc cttcaccccc ggcgcgcgtt
     1261 cgacgccgga cgagacgtcg agcgcctcgg cgccgctcag gcgcacggct tcggcgacat
     1321 tgcccgcatc cagcccgccg gccagaaacc acggcttcgc ccccgtccag ccgcgcatga
     1381 tcgtccagtc gaagcgcagc cccatgccgc cgggcagggc ggcgcctttc ggcgtcctgg
     1441 cgtcgaacag caggtaatcg gccgcatcct cataggcggg cgcggcggcg aggtcggcgg
     1501 cttcggccac gctgaccgcc ttgatcaccg gcaatccggc gcgcgcgcgg acggcggcga
     1561 cgcgcgccgg cgattccttg ccgtgcaact ggatcaggtc cggcttgacc tcatggatca
     1621 gcgcgtcgat cagcgcgtcg tccgggtcga cgaccacgct gacccgccgc gcgtccggcg
     1681 tctcggcgcg gatcgcccgc agcagccccg ccatgcggtc gaggccgaga tggcgcgggc
     1741 ttttgccgaa gtggatgaaa ccgagatagg cggcgccgtg gtccagcgcc gcgcgcgccg
     1801 cttccggcgt cgaaattccg caaatcttta tgtccggttg cataaaaaca cctcgattta
     1861 accgcctagg tggagcaacc tttaacaaaa atcaagattt taactctaat tttcagtctt
     1921 aattaaaccc gccgcgtcac aggcgggata aaatataaaa ataccataaa ataaaacatg
     1981 gtagtcaaca tggggcaggc aaacaaaaat gaacgactac gttctgttga gcgatatcag
     2041 taatggcaat tcacttaagc tggccctggc gagaccgggc tcgcggccgg aaacgacgac
     2101 ctattacgca tgtaacgact ttgacggctt caaggacgcc atttccgact ttctgaacga
     2161 caagggccgt ccgtctttgg cgggcgcggc gatttccgcc gccggatggg agcgcggcgg
     2221 ggtgatccgg ctgcccaatc acggcttttc gatcagccgc ggcgacatgc gcgatttcct
     2281 cagcatccag cgcgtcaatc tggtcaacga cttcgtcgcc aaggcgctgg ccattccgcg
     2341 cctgcgcgag tccgagcgcc agcagatctg cggcggcgaa ccgatggagg agcaggtgat
     2401 cgccgtgctg gggccgcata acgggctggg catgtcggcg ctggcgccgg acggcatggg
     2461 ggcgtggacc gccatgccga ccgagggcgg ccattccgat ctggcggcga ccaacgcgct
     2521 ggaggatcag gtgctgggcg tgctgatgcg caaatacggc catgtgtcgc gcgaacgggt
     2581 gatctcgatt ccgggactgg tcgacatctg gcgcgcgctg gccgagatcg acggcgacga
     2641 catcgacgtg ccggggccgg aggagatcat cgcccgcgcc atggccgagg acacgcgcgc
     2701 ccatcaggtc atcgatcttt ccatgggctg gttcgcggcg atggccgccg acgtggcgct
     2761 gatcctgggc gcgcgcggcg gcgtctatct ggcgggcgac ctgctggaca tgatcggcga
     2821 tctgttcgac gtcgaagcct tcgtcaaacg ctatacggac aagggccgcc tgagcggcta
     2881 cgtcgccgaa atccccgtgt tccgcgccac ggcgcaggac atggaggtga tcggactggc
     2941 caccctgttc gactaaagcc cccaaggaac cccgcgcaag gaaccccgcg ccatgaccgc
     3001 ttacgtgctg ctgtgcgact tcacgttcgg ctcctatatc cggctggcgc tggccgaggc
     3061 cgggcggcgg cccgaaccgc cctccttgcg cgcctgcgcc gaccgcgccg ggttcgatgc
     3121 gcaggtcatg ggctttctgg ccgaccacgg ccatccgcgc ctgatcggcg ccgccctgtc
     3181 gacgcgcggc tgggagacgc acggcgtgct gcacctgccc gatgagggga tgcatctgac
     3241 gcgcgacaat gtccgcgggc t
//
LOCUS       DASVLL010000698         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_160345,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000698 DASVLL010000000
VERSION     DASVLL010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160345"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VF402_11465"
     CDS             <1..178
                     /locus_tag="VF402_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7800963.1"
                     /translation="RAVLVITGKGVAETGLLKRMAPEWLADPALSHIVAGLSPAHARH
                     GGAGALYVALKRRG"
     gene            complement(277..1272)
                     /locus_tag="VF402_11470"
     CDS             complement(277..1272)
                     /locus_tag="VF402_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX7800964.1"
                     /translation="MTAAYDITAVGHAIVDVLAPADEAFLAAHDLHKGAMTLIDQHRA
                     HSLFDQMAEGEMASGGSAGNTIAGAASFGATCGYIGKVAHDSLGEFFSRDLKTMGVDF
                     RTPVLHDDPAATGRCLINVTADGQRTMATFLGAAALISPDDLDADMIRASQITYLEGY
                     LFDTPSGRETFAKAAQIARAAGRKTAITLSDMFVVNRWREDLLAFIDRHIDLVFANES
                     ELLGLFQTDDFYKALRYLRARVDLAFVTRSEKGSVVAKLDDTHTVEAAPVAKVIDTTG
                     AGDQYAAGVMYGLTQGLHVTTCARLGSLAAAEVISHFGPRPLVSLADLAKEAALV"
     gene            1329..>1887
                     /locus_tag="VF402_11475"
     CDS             1329..>1887
                     /locus_tag="VF402_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800965.1"
                     /translation="MSKRLLILTPNPDHPSHHGRWPYVLDAYREALAGLDLEIFDQPW
                     SQPVAEDYDLILPLVAWGYHNAPGSFVGAMTALGAAHRRVFNPPEIVCWNVDKSYLRD
                     LAEAGIRIVPTLFADAVTDAEVARARQDFGQDALVLKPRISAGAKNTLVWDGERLADV
                     VPADAKAVPPVGPAMIQPYMPAIASE"
BASE COUNT          303 a          601 c          651 g          332 t
ORIGIN      
        1 ccgcgccgtg ctggtcatca ccggcaaggg cgtggccgaa accggcctcc tgaaacgcat
       61 ggcgccggaa tggctggccg acccggcgct ttcccacatc gtcgcgggcc tgtcccccgc
      121 ccacgcccgc cacggcggcg caggcgcgct ctatgtcgcg ttgaagcggc ggggttagag
      181 cgcttttcga tctgattgca tcagatcggc gctctaagtc tttgttttgt cgctcttcct
      241 tatccaaaaa gtggagccac tttttgggga aacgctctaa accagcgccg cttccttcgc
      301 caggtcggcc agcgagacca gcggacgggg gccgaaatgg ctgatgacct cggcggcggc
      361 caacgagccg agccgggcgc aggtggtgac gtgcaggccc tgcgtcaggc cgtacatcac
      421 gcccgccgca tattggtcgc ccgcgccggt ggtgtcgatc accttcgcga ccggcgcggc
      481 ttcgacggtg tgggtgtcgt ccagcttggc gacgaccgag cctttttccg agcgcgtgac
      541 gaaggccaga tcgacgcggg cgcgcaggta gcgcagcgcc ttatagaagt cgtcggtctg
      601 gaacaggccc agaagctcgg actcgttggc gaagacgagg tcgatatggc ggtcgatgaa
      661 ggccagcaga tcctcgcgcc agcggttgac cacgaacatg tccgacaggg tgatcgccgt
      721 cttgcggccc gccgcgcgcg cgatctgcgc ggccttggcg aaggtttcgc ggcccgacgg
      781 cgtgtcgaac agatagcctt cgagataggt gatctgcgag gcgcggatca tgtcggcgtc
      841 gagatcgtcc gggctgatca gcgccgcggc gccgaggaag gtcgccatcg tcctttgccc
      901 gtccgccgtg acgttgatca ggcagcggcc cgtggcggcc ggatcgtcgt gcagcaccgg
      961 cgtcctgaaa tcgacgccca tcgtctttag gtcgcggctg aaaaattcgc ccagggaatc
     1021 atgcgcgacc ttgccgatat agccgcatgt cgcgccgaac gaggccgcgc ccgcgatggt
     1081 gttgcccgcc gagccgcccg aagccatctc gccctcggcc atctggtcga acaggctgtg
     1141 cgcgcggtgc tggtcgatca gggtcatcgc gcccttgtgc aggtcgtgcg ccgccagaaa
     1201 cgcctcgtcg gcgggggcca gaacgtcgac aatggcgtgg ccgacggcgg tgatgtcgta
     1261 agcggcggtc atgaaacgtc tcgaaattcc cgttgcagtt cccaccccta tggatcaata
     1321 agacggcgat gagcaagcgt ttattgatcc tgaccccgaa cccggaccac ccgtcgcacc
     1381 acggacgctg gccctatgtg ctggacgcgt accgcgaggc gctggccggg ctggaccttg
     1441 agattttcga tcagccgtgg tcgcagccgg tcgcggagga ttacgatctg atcctgccgc
     1501 tggtggcgtg gggctatcat aatgcgccgg gcagcttcgt cggcgcgatg acggcgctgg
     1561 gggcggcgca tcggcgcgtg ttcaatccgc cggagatcgt gtgctggaac gtcgataaat
     1621 cgtacctgcg cgatctggcc gaggcgggga ttcgcatcgt gccgaccctg ttcgccgatg
     1681 cggtgacgga cgccgaggtc gcccgcgcgc gtcaggattt cgggcaggac gcgctggtgc
     1741 tgaaaccgcg catttcggcg ggcgccaaga acacgctggt ctgggacggc gaacggctgg
     1801 cggatgtcgt gcctgcggac gccaaggccg tgccgccggt gggaccggcg atgatccagc
     1861 cctacatgcc cgccatcgcg tccgaag
//
LOCUS       DASVLL010000699         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_103641,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000699 DASVLL010000000
VERSION     DASVLL010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103641"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VF402_11480"
     CDS             <1..741
                     /locus_tag="VF402_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7800966.1"
                     /translation="DWSFGPPQLQRYNGFGSMEIQGAAAQGQSNGKAMQVMESLVKQL
                     PGAWGFEWTGLSLQEQESGQQAPALYALSLLVVFLLLAALYESWSIPFAVIFVVPLGI
                     FGALVGTWLRGLDNDIYFQVGLLATMGLSAKNAILIVEFAKILHEEGRGLIAATLEAV
                     RIRLRPIIMTSLAFTFGILPLALANSAGSSAQHAIGTGLIGGVLAATFLAIFFVPLFF
                     VLVQRFFKQDRLPQDIEEKRAIKEGRHV"
     gene            734..2125
                     /locus_tag="VF402_11485"
     CDS             734..2125
                     /locus_tag="VF402_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7800967.1"
                     /translation="MSKLILPSLLALAIGVSGCTLAPRYERPGLPVAQNWSVPAPAAA
                     SDARLSWRDLFTDPALQKTIELALANNRDLRVATLNVQKARAQYGVTRAGLLPSLDAT
                     GSGAKSHLNTPTAATDANGDSESYAAGLSASWELDLFGRVQSLKNAAREDVFAAQENR
                     NAAQIALIAAVADAWIGLAADQDALNLSQQTYASLNDAYGIAQKQAGIGAISDLDLNQ
                     IKTEMEQARVNAAAAATAVDQDKAALTLLLGANLPADLTPTGLSGDVVANDIPVGLPS
                     GVLLNRPDVLAAEHDLKAYNADIGAARAAFFPSISLTGATGAASSALSDLFRSASGKW
                     SYGANLSVPIFAGGANLNALRAAKVSRDIAVAQYEQAIQSAFSDVNKALAVRARIDER
                     LDAQTAATGAAQTSLDLSQARYRAGADSYLTLMTAQRTLYASQSALIGLKALKADNLV
                     ALYAAIADDRSLQ"
     gene            2355..>2538
                     /locus_tag="VF402_11490"
     CDS             2355..>2538
                     /locus_tag="VF402_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800968.1"
                     /translation="MIRLRAFLFTGLLAAAVPVAASHAQTLNNPAQWGREHARAVAAN
                     DPANDPDNPASSNALNL"
BASE COUNT          428 a          896 c          805 g          409 t
ORIGIN      
        1 gactggagct tcggcccgcc gcagttacag cgctataacg gcttcggctc gatggagatt
       61 caaggcgccg ccgcgcaggg ccagtcgaac ggcaaggcga tgcaggtcat ggaaagcctt
      121 gtcaagcaac tgcccggcgc ctggggcttc gaatggaccg gcctttcctt gcaggaacag
      181 gaatcgggcc agcaggcgcc cgcgctctac gcgctgtcgc tgctggtggt gttcctgctg
      241 ctggcggcgc tgtatgaaag ctggtcgatc ccgttcgcgg tcatcttcgt ggtgccgctg
      301 ggcattttcg gcgcgctggt cggcacatgg ctgcgcgggc tggacaacga catctatttt
      361 caggtcgggc ttctggccac catggggctg tcggccaaga acgcgatcct gatcgtcgaa
      421 ttcgccaaga tcctgcacga ggaaggccgg gggctgatcg ccgccacgct ggaagccgtg
      481 cgcatccggt tgcggccgat catcatgacc tcgctggcct tcaccttcgg catcctgccg
      541 ctggccctgg ccaattcggc gggctcgtcg gcgcagcacg ccatcggcac cggcctgatc
      601 ggcggggtgc tggcggcgac cttcctggcc atcttcttcg tgccgctgtt cttcgtgctg
      661 gtgcaacgct tcttcaagca ggaccgcctg ccgcaggaca tcgaggaaaa acgcgccatc
      721 aaggagggcc gtcatgtcta agctgatcct cccctccctg ctcgctctgg ccatcggcgt
      781 ctccggctgc acgctggcgc cgcgctatga gcggcccggc ctgccggtgg cgcagaactg
      841 gtccgtcccg gcgcccgccg cggcatcgga cgcgcggctg tcgtggcgcg atctgttcac
      901 cgatccggcg ctgcaaaaga ccatcgagct ggctctggcc aacaaccgcg acctgcgcgt
      961 ggcgacgctc aacgtgcaaa aggcgcgcgc ccagtacggc gtcacgcgcg cgggcctgct
     1021 gccgtcgctg gacgccacgg ggtccggcgc gaaaagccac ctcaacacgc cgaccgccgc
     1081 gaccgacgcg aacggcgaca gcgaaagcta cgccgccggt ctgagcgcgt cgtgggagct
     1141 ggacctgttc gggcgcgtgc agagcctgaa aaacgccgcc agagaggacg tcttcgccgc
     1201 gcaggaaaac cgcaacgcgg cgcagatcgc gctgatcgcc gccgtcgcgg acgcctggat
     1261 cggcctcgcc gccgatcagg acgcgctcaa cctgtcgcag cagacctacg ccagcctgaa
     1321 cgacgcctac ggcatcgcgc agaagcaggc gggcatcggc gccatttccg acctcgacct
     1381 caaccagatc aagaccgaga tggagcaggc gcgcgtcaac gccgccgccg ccgccaccgc
     1441 cgtcgatcag gacaaggcgg ccctgaccct gctgctcggc gcgaacctgc ccgccgacct
     1501 gacgccgacc gggctgtccg gcgacgtggt ggcgaacgac atcccggtcg gcctgccgtc
     1561 cggcgtgctg ctgaaccgcc cggacgtgct ggccgccgaa cacgacctga aggcgtacaa
     1621 cgccgacatc ggcgcggcgc gcgcggcctt cttcccgtcg atcagcctga ccggcgcgac
     1681 cggcgcggcc tcgtccgccc tgtcggacct gttccggtcg gccagcggca aatggtccta
     1741 cggcgccaat ctcagcgtcc cgatcttcgc cgggggcgcc aatctcaacg ccctgcgcgc
     1801 cgccaaggtc agccgcgaca tcgccgtggc gcagtacgag caggcgatcc agagcgcgtt
     1861 ttcggacgtg aacaaggcgc tggccgtgcg cgcccgcatc gacgagcggc tggacgccca
     1921 gaccgccgcc accggggcgg cgcaaacctc gctcgacctg tcgcaggcgc gttaccgcgc
     1981 cggggccgat tcctacctga ccctgatgac cgcgcaacgc acgctctacg cctcgcaatc
     2041 ggcgctgatc ggcctgaagg cgctcaaggc cgacaatctg gttgcgctct acgccgccat
     2101 cgcggacgac cggagcctgc aataacgcct agagcgcttt tcgatctgat agaatcagat
     2161 cggcgctcta agtctttgtt ttgtcgctct tccttatcca aaaagtggaa ccactttttg
     2221 gggaaacgct ctaacggggg cgcttgcatg aacgccgtga cacaagcgcc ctcaaagctt
     2281 cttaatcgcg tcacgggaaa aacaggaaac aggcgcatcg tcttgccggg ctggcgaatg
     2341 aaggagatgt cgtcatgatc cgtttacgtg cgttcctttt caccgggctt ctcgccgccg
     2401 ccgttcctgt cgcggccagc catgcgcaga cgctcaacaa tccggcgcaa tggggccgcg
     2461 aacatgcgcg cgccgtcgcg gcgaacgatc cggccaacga ccccgacaat cccgccagct
     2521 ccaacgcgct gaacctca
//
LOCUS       DASVLL010000700         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_264824,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000700 DASVLL010000000
VERSION     DASVLL010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264824"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF402_11495"
     CDS             complement(<1..255)
                     /locus_tag="VF402_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800969.1"
                     /translation="MPHTLAWLRVWLPVWPHTDAPVWLLAGLPPVMAALVFAVWKIWR
                     VQIRIWLIPAAELRACAAGLGAAHGAKAEAVALDLSLKAWL"
     gene            634..1380
                     /locus_tag="VF402_11500"
     CDS             634..1380
                     /locus_tag="VF402_11500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7800970.1"
                     /translation="MKDMRFAGRSVIVTGGLGGIGRACAQGFAREGARLMLADLRVEG
                     AAEAVAELKRLGAADARVEACDVSREADVARVCRAAVDLYGGVDVLVNVAGMMIYKAI
                     EELSFEDWSRLMGVNFFGAAMFTREAFRVMKPGGAIVNVSSIHERQTSPLVAPYAAAK
                     AALGSLTRTASIEGRAKGIRVNAVLPGAVDTMMLWASPNLKSGAETLEPGDVGQPSQI
                     ADAVLYLAGAQAGFVTGASLNVDGGRLAKL"
     gene            complement(1377..2804)
                     /locus_tag="VF402_11505"
     CDS             complement(1377..2804)
                     /locus_tag="VF402_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEX7800971.1"
                     /translation="MLIDASKPALAAPGAHRYNLVYIWAISATAALGGLLFGYDWVVI
                     GGAKPFYEPYFRLTSDAQIGWANSCALLGCLIGSILSGALSDRLGRKILLVAAAFLFG
                     VSSLLTGWAHGFDAFVLWRILGGVAIGIASNVSPTYIAEIAPAAWRGRLVTLNQLTIV
                     IGILAAQIVNLMIAGHVADNATPEMIRASWQGQFGWRWMFTAVAAPSLVFFVCAFLVP
                     ESPRWLIKTGANDRARRILTRIGGESYADREMREVADGLSREASEQGSWGELLRPAVL
                     GLVLMGIGLAVLQQWSGTNVIFNYAEDIYRGAGYDLSGVMMNIVITGAINLIFTLTAT
                     GLVDRIGRRTLMLWGSGGIAVLHIALGLAFFFGLKGPLVLALTLGVIAVYAMSLAPVT
                     WVLLSEIFPNRIRGLAMSVAVSALWIACFAVTFTFPILNQRLGAAGTFWCYGVFCAIG
                     FLFILRRVPETRGKSLEDIERELAG"
BASE COUNT          544 a          939 c         1054 g          438 t
ORIGIN      
        1 gagccacgcc ttcaggctga ggtcgagcgc gacggcctca gccttcgcgc cgtgcgccgc
       61 gcccagcccg gccgcgcacg cgcgcagttc ggcggcgggt atcagccata tccgtatctg
      121 gacgcgccag attttccaga cggcgaagac gagggccgcc atcacgggcg gcaggcccgc
      181 gagcagccag acaggcgcat ccgtgtgcgg ccagacaggc aaccagacgc gcagccatgc
      241 gagcgtatgc ggcatggacc cgaaaccttc ccccgaaggc ccggcaagcg gtgcgccccc
      301 gccttaggcc tcctctgaca atcctggtga tcggggagtt agaaaaccgg gcattactcg
      361 gtccctgtga cctttagccg gggcctggac atgcgccaca ggctccccga ccgcatgagt
      421 cgaaggaagg cgcgccggaa tggccggcgt cgaaccagta atgccttggg gttttctaag
      481 accccggagc cgtgcgttcg gctccgtgac gagcataggc tgaaagcggc ggccaagtca
      541 ttacgaaaat ttcggcgcga gggtgggcaa gccttgcgcg gcggggcgga gttgggcatg
      601 tcggagggag acggaaaaca ggacaggcgg aggatgaagg acatgcgttt cgcgggcaga
      661 tcggtgatcg tgacgggcgg tctgggcggc atcggcaggg cgtgcgcgca gggcttcgcg
      721 cgcgaggggg cgaggctgat gctggccgac ctgcgcgtgg agggggcggc ggaggccgtc
      781 gcggagctga aaaggctggg cgcggcggat gcgcgcgtcg aggcgtgcga cgtgtcgcgc
      841 gaagccgacg tggcgcgcgt ctgccgcgcc gccgtcgacc tgtatggcgg cgtcgacgtg
      901 ctggtcaatg tcgcgggcat gatgatctac aaggccattg aggagctgag tttcgaggac
      961 tggtcgcggt tgatgggcgt caatttcttc ggcgcggcga tgttcacgcg cgaggcgttc
     1021 cgcgtcatga agccgggcgg cgcgatcgtg aacgtgtcga gcattcatga gcgtcagacc
     1081 tcgccgctgg tcgcccctta cgcggcggcc aaggcggcgc tgggttcatt gacgcgcacg
     1141 gcgtccatcg agggccgggc gaagggaatc cgcgtcaacg ccgtgctgcc cggcgcggtc
     1201 gatacaatga tgctgtgggc cagtccgaac ctgaaatcgg gcgccgagac gctggagccc
     1261 ggcgatgtcg gccagccgtc gcagatcgcc gacgcggtgc tgtatctggc cggcgctcag
     1321 gcgggcttcg tcaccggcgc cagcctcaat gtcgacggcg ggcggctggc gaagctttag
     1381 ccggccagct cgcgctcgat gtcctccagc gacttgcccc tggtttcggg cacacgcctg
     1441 aggatgaaca ggaagccgat ggcgcagaaa acgccgtagc accagaaggt tcccgccgcg
     1501 ccgaggcgct ggttcaggat cgggaaggtg aaggtgaccg cgaaacacgc gatccacagc
     1561 gccgacaccg ccaccgacat ggccagaccc cggatgcggt tggggaagat ttccgacagc
     1621 agcacccagg tcaccggcgc cagcgacatg gcgtagacgg cgatgacgcc cagcgtcagg
     1681 gccagcacca gcggcccttt cagcccgaag aagaaggcca ggcccagcgc gatatgcagg
     1741 acggcgatgc cgcccgatcc ccacagcatc agggtgcgcc gcccgatccg gtcgaccagc
     1801 ccggtggcgg tcagggtgaa gatcaggttg atcgcgccgg tgatgacgat gttcatcatc
     1861 acgccgctca ggtcgtagcc cgcgccgcga tagatgtcct cggcgtagtt gaagatgaca
     1921 ttggtgccgc tccattgttg cagcacggcg aggccgatgc ccatcagcac gaggcccagc
     1981 acggcggggc gcagaagctc gccccacgag ccttgttcgg aggcttcgcg gctcaggccg
     2041 tcggcgacct cgcgcatttc ccgatcggcg taggattcgc cgccgatgcg cgtcaggatg
     2101 cggcgggcgc ggtcgttcgc gccggtcttg atcagccagc gcgggctttc cggcacgagg
     2161 aaggcgcaga cgaagaagac cagagacggc gccgcgacgg cggtgaacat ccagcgccag
     2221 ccgaactggc cctgccatga ggcgcggatc atctccggcg tggcgttgtc ggcgacatgg
     2281 cccgcgatca tgagattgac gatctgcgcc gccaggatgc cgatcacgat ggtgagctgg
     2341 ttcagcgtca ccagccgtcc gcgccacgcc gccggggcga tctcggcgat ataggtcggc
     2401 gacacgttcg aggcgatgcc gatggcgacg ccgcccagga tgcgccacag cacgaaggcg
     2461 tcgaagccat gcgcccagcc ggtcagcagc gacgagacgc cgaacaggaa ggcggcggcg
     2521 accagcagga tcttgcgccc cagccggtcg ctgagcgcgc ccgacaggat cgagccgatc
     2581 aggcagccga gcagggcgca actgttcgcc cagccgatct gggcgtccga ggtcaggcgg
     2641 aaatacggct catagaacgg cttggcgccg ccgatcacca cccagtcgta gccgaacaga
     2701 agcccgccca gcgccgccgt cgcggagatc gcccagatat agacgagatt ataacggtgc
     2761 gcgccgggcg cagcgagggc gggcttggag gcgtcgatca gcatggcggt ccctgtcggt
     2821 tatgccgatc agttaaattc gatcacaagc gaaagtcaat aaatcataaa atatgatcat
     2881 tattgaccga agtggcgcgg cgcgcgcggc gttaagaatt ccggcccgcc gcccttgacc
     2941 ccgccagccc cttcctatat aggccgctga tcatt
//
LOCUS       DASVLL010000701         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_319794,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000701 DASVLL010000000
VERSION     DASVLL010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319794"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1547
                     /gene="gabT"
                     /locus_tag="VF402_11510"
     CDS             249..1547
                     /gene="gabT"
                     /locus_tag="VF402_11510"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX7800972.1"
                     /translation="MTRNADLAARRAAAVPRGVGHATTIYAARAENAELWDADGRRYI
                     DFAGGIAVLNTGHRHPRVQAAAMAQMSAFTHTAFQVAPYEPYIALAERLNALAPVAQP
                     AKTLFMTTGAEAVENAVRIARAYTRRPNVVAFTGGFHGRTALTSALTGKVVPYKKDLG
                     PALAGVYHLPFPVESHASKEGGVEVADTLKMLDYLFRADVAPDTVAAIVIEPVQGEGG
                     FLIAPPELMRALRERCDQHGILLIADEVQTGFGRTGKLFAMEHYDVRPDLMTMAKSLA
                     GGYPLSGVVGRADIMDAPEPGGLGGTYGGNPVACAAALAVLDVIEEERLLDAANRQGA
                     KMKARLEALAQRNDIVPITAVRGLGAMIAFDIVTDRGGHAPDAAAAKAVTAKAQDNGL
                     ILLSCGVFANTIRLLCPLTASDAILDEGLARLEAALTLQA"
     gene            complement(1538..>2140)
                     /locus_tag="VF402_11515"
     CDS             complement(1538..>2140)
                     /locus_tag="VF402_11515"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEX7800973.1"
                     /translation="PFPQIADNYYPGVRRMLRQGEAAYHYVKSVCGVLPPVLQRVYGV
                     MRFHVREASFSMVTQRPETTQLIQRVPHFDTLNPADFAVLHYLSPTPFGGTGFYRHRR
                     TGFERVSQARYQLYLDALDEDLRAYGPPEARYITGSSPAFERLGGSEGRFNRLIIYPG
                     ALLHCADIDPSFTFASDPRTGRLTGNIFIRAETVTDASQA"
BASE COUNT          387 a          749 c          668 g          336 t
ORIGIN      
        1 cttcatgggc gcggccatcg tcttcgtcgc ctgcgccttc ctggcgtgga tcctgcccaa
       61 gccgcaaggc ccggtggaca tgtcgcaggt gcattgacgc ccgagattgt tcccttacct
      121 gcaaggcggc tccgtttccc ctcggagccg cctctttttt atgcggtttc atgcttttgg
      181 cggtccacaa atccaacaaa ttgtgatcac aatatgctat ctgacgccaa gccatcagga
      241 gcgccgccat gacccgcaac gccgatctcg ccgcccgccg cgccgccgcc gttccgcgcg
      301 gcgtgggcca cgccaccacc atctacgccg cgcgcgccga gaacgccgaa ctgtgggacg
      361 ccgacggcag gcgctatatc gacttcgcgg gcggcatcgc cgtgctcaac accggccacc
      421 gtcaccccag ggtgcaggcc gccgcgatgg cgcagatgag cgccttcacc cacaccgcct
      481 ttcaggtcgc gccttacgag ccctatatcg cgctggccga acgcctcaac gcgctggcgc
      541 ccgtcgccca accggccaag accctgttca tgaccacggg cgccgaggcg gtcgagaacg
      601 cggtcaggat cgcgcgcgcc tacacccggc gtccgaacgt cgtcgccttc accggcggct
      661 ttcatggccg caccgccctg acctcggccc tgaccggcaa ggtcgttccc tacaagaaag
      721 acctcggccc ggcgctggcg ggcgtctatc acctgccctt cccggtcgag agccatgcca
      781 gcaaggaggg gggcgtcgag gtcgccgaca cgctgaaaat gctcgactac ctgttccgcg
      841 ccgacgtcgc gccggacacg gtcgccgcca tcgtcatcga gccggtgcag ggcgaaggcg
      901 gcttcctgat cgcgccgccg gaactgatgc gcgcccttcg cgaacgctgc gaccaacatg
      961 gcatcctgct gatcgccgac gaagtgcaga ccggcttcgg ccgcaccggc aagctgttcg
     1021 cgatggagca ttacgacgtc aggcccgacc tgatgacgat ggcgaaatcg ctggccggcg
     1081 gctatccgtt gtcgggcgtc gtcgggcgcg ccgacatcat ggacgcgccg gagccgggcg
     1141 gcctcggcgg cacctacggc ggcaatccgg tcgcctgcgc cgccgccctg gccgtgctgg
     1201 acgtgatcga agaggaaagg ctgctcgacg ccgccaaccg tcagggcgcg aagatgaagg
     1261 cgcgtctgga ggcgctcgcg caacgcaacg acatcgtccc gatcaccgcc gtccgcggcc
     1321 tcggcgccat gatcgccttc gacatcgtga ccgaccgcgg cggccacgcc cccgacgcgg
     1381 cggccgcgaa agccgtcacc gccaaggcgc aggacaacgg cctgatcctc ctgtcctgcg
     1441 gcgtgttcgc caacaccatc cgcctcctct gcccgctcac cgcctccgac gcgatcctcg
     1501 acgagggcct ggccagactg gaggcggcgc tgacgcttca ggcttgagag gcgtcggtta
     1561 cagtctctgc gcggataaag atattccccg tcaaacggcc cgtgcgcgga tcggaagcga
     1621 aggtgaatga gggatcgata tccgcgcaat gcaacagcgc gcccggatag atgatcaagc
     1681 ggttaaaccg gccctcacta ccgcccagac gctcgaatgc gggactggag ccggtgatat
     1741 agcgcgcttc cggcgggcca tatgcgcgca gatcctcatc gagagcgtcc agataaagct
     1801 ggtagcgcgc ctgtgaaacc cgctcgaacc ccgtgcgccg gtgacgataa aagccggttc
     1861 cgccgaaggg cgtgggtgac aaataatgca ggaccgcgaa atcggcggga ttcagcgtat
     1921 cgaaatgcgg cacgcgctgg ataagctgcg tcgtttccgg cctttgcgtc accatggaga
     1981 agctggcctc gcgaacgtga aaccgcatca cgccatagac gcgctgaaga acgggcggca
     2041 agacgccgca gaccgatttg acataatgat aagccgcttc accttgccgc aacatccgcc
     2101 ggacgccggg gtaatagttg tcggcgattt gcggaaaggg
//
LOCUS       DASVLL010000702         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_47469, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000702 DASVLL010000000
VERSION     DASVLL010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47469"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1319)
                     /locus_tag="VF402_11520"
     CDS             complement(132..1319)
                     /locus_tag="VF402_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7800974.1"
                     /translation="MLNRRHLLAGLGVTAAFGGAIPALARAAGARDPRLVVIVLRGAM
                     DGLSAVPPVGDPDFASLRGAFPQDGDVKLDTTFSLHPSLSHFARQYQAGQALVVHAVA
                     SPYRDRSHFDGQDVLESGMTAPGHTESGWLNRALLALPGAQKATGQTLKGLGVGGVTP
                     LIIRGPAPVLGWAPSSLKPADADLPGRLQSLYGESDPALAATLKEAIDTGRIIGPNSM
                     AGAYGGPGDPKTMEAMAQGAAKLLAQDDGPRIAALAFEGWDTHATEPQRLARQLQGLD
                     AAYAAFETALGPVWKDTAILTVTEFGRTAAVNGTNGTDHGTATVALLTGGAVKGGRVV
                     GDWPGLKPAQLYQNRDLAPTTDLRSVAKGLLAGFYDLPESALAKDVFPDSADARPMRD
                     LIA"
     gene            complement(1321..2730)
                     /locus_tag="VF402_11525"
     CDS             complement(1321..2730)
                     /locus_tag="VF402_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEX7800975.1"
                     /translation="MAQTLYDPAIALSRFGLGATARGVGAVGDARAVLQSEIAQGPPQ
                     PQNLDATPNLLSALYAYQKEKRQARQAANAGDANGPKKPGRDDNPIRPAALAEIAARF
                     DGTVKSPVIGFNERMAQFWANHFAVSTKKDQALAITAGSFEREAIRPHVFGRFSDLLL
                     AVETHPAMLLYLDNTTSIGPDSPAARRGKGGGKGGGKRGLNENLAREIMELHTLGVGS
                     GYTQTDVTTFAKVITGWSVARDNERDGTPPGTFIFRRYAHEPGAQTILGKRYAQDGMD
                     QGKAVLLDLARHPATAQHIATKLARAFVADDPPPALVTRLRETFRRSDGDLAEVSRVL
                     IEAPESWSPQRAKMRSPLEYTCALIRATDIAPKPQAIEAALNAMGQPFWQPAGPNGYP
                     DAVAAWNSPEGMATRMDVANSYADMLARRRPDADARVLAQSLFGPQLSAATARAVARA
                     ETQPQGLALLFMSPEFMRR"
     gene            complement(2809..>3517)
                     /locus_tag="VF402_11530"
     CDS             complement(2809..>3517)
                     /locus_tag="VF402_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX7800976.1"
                     /translation="LEASGLYVAGRGASPNAAGVYELDAAVMTGHDQRCGAVAALQGY
                     ASRVHVARAVMERTPHVLLAGDGAARFAAGQGFEALADPDAYFTRAGRFESNHPPGAL
                     AHGTVGCVCRDAHGRLAAATSTAGVFGKLPGRVGDTPLIGAGTWADVGAAVSCTGQGE
                     YFIRANAAAQVAFRHAAGEALNAAADAVLARIASMGGEGGLIAVDARGGICAPFRSQG
                     MKRAWFSGDAEIVSEAF"
BASE COUNT          480 a         1199 c         1284 g          554 t
ORIGIN      
        1 gcgctgatgg agcgcacggc gatggaaaag gggatcgacc tgtcgcacgt gccgccgggc
       61 gcctcgctcg acggcataga caaggtttgt caatagttca ggtctggcga tagttcaggt
      121 ctggcgatag ttcaggcgat cagatcgcgc atcggcctcg catccgcgga gtcggggaag
      181 acgtccttcg ccagcgcgct ttcgggaaga tcgtagaatc cggccagcag gcccttggcc
      241 accgagcgca aatccgtggt cggggccaga tcgcggttct gatagagctg cgccggtttc
      301 aggccgggcc agtcgccgac cacgcgcccg cccttcaccg cgccgccggt cagcaacgcc
      361 accgtggccg tgccgtggtc ggtgccgttg gtgccgttga cggcggcggt gcggccgaac
      421 tcggtcacgg tcaggatggc ggtgtccttc cacaccggcc ccagcgccgt ctcgaacgcg
      481 gcgtaggcgg cgtccagccc ttgcagttgc cgcgccagcc tttgcggttc ggtggcgtgc
      541 gtgtcccagc cctcgaaggc cagcgccgcg atgcgcggcc cgtcgtcctg cgccagcagc
      601 ttggccgcgc cctgcgccat cgcctccatc gtcttgggat cgcccggccc gccataggcg
      661 cccgccatgc tgttcgggcc gatgatccgc ccggtgtcga tggcttcttt cagcgtcgcg
      721 gccagcgccg gatcgctttc gccgtacagg ctttgcagcc gtccgggcag gtcggcgtcg
      781 gcgggcttga ggctggacgg cgcccagccc agcaccggcg cggggccgcg gatgatcagc
      841 ggcgtgacgc cgccgacgcc taagcctttg agcgtttgcc ccgtggcctt ctgcgcgccg
      901 ggcagggcga gcagcgcccg gttgagccag cccgattcgg tgtggccggg cgcggtcatg
      961 cccgattcca gcacgtcctg accgtcgaag tgcgagcggt cgcgataggg cgaggcgacg
     1021 gcgtggacga cgagggcctg acccgcctga tactgccgcg cgaaatggct cagcgacggg
     1081 tgcagcgaga aggtcgtgtc cagcttcacg tcgccgtcct gcggaaaggc gccgcgaaga
     1141 ctggcgaaat cggggtcgcc gacgggcgga accgcgctca gcccgtccat cgcgccgcgc
     1201 agcacgatga cgacgagacg cggatcgcgc gctccggcgg cgcgggcgag ggcgggaatg
     1261 gcgccgccga aggcggccgt gacgccgaga ccggcgagaa gatgacgacg attcaacata
     1321 tcagcgcctc atgaattcgg gggacatgaa caacagggcc aggccctgcg gttgcgtttc
     1381 ggcgcgcgcc acggccctgg cggtggccgc gctcagttgc gggccgaaca ggctttgcgc
     1441 caatacgcgg gcgtcggcgt ccgggcggcg gcgcgccagc atgtcggcgt agctgttggc
     1501 gacgtccatg cgcgtggcca tgccttcggg ggagttccac gccgccacgg cgtcgggata
     1561 gccgttcggc ccggcgggct gccagaaggg ctggcccatc gcgttgagcg cggcttcgat
     1621 ggcctgcggc ttgggcgcga tgtccgtggc gcggatcagg gcgcaggtgt attccagcgg
     1681 cgagcgcatc ttggcgcgct gcggcgacca gctttcgggc gcttcgatca ggacgcgcga
     1741 cacctcggcc agatcgccgt cgctgcgccg gaacgtctcg cgcaggcgcg tcaccagggc
     1801 gggcggcgga tcgtcggcga cgaaggcgcg cgccagcttg gtcgcgatat gctgcgcggt
     1861 cgccgggtgg cgggccaggt cgagcagcac cgccttgccc tgatccatgc cgtcttgcgc
     1921 gtagcgtttg ccgaggatgg tctgcgcgcc cggttcgtgc gcgtagcggc ggaagatgaa
     1981 ggttccgggc ggcgttccgt cgcgttcgtt gtcgcgcgcc acgctccagc cggtgatgac
     2041 cttggcgaag gtggtcacgt cggtctgggt gtagccggag ccgacgccca gtgtatgcaa
     2101 ctccatgatt tcgcgcgcca gattctcgtt gaggccgcgc ttgccgccgc ctttcccgcc
     2161 gccttttcct ctgcgggcgg cgggcgaatc cggcccgatc gaggtcgtgt tgtcgagata
     2221 gagcagcatg gccggatgcg tctcgaccgc cagcagcagg tcggagaacc ggccgaagac
     2281 gtgcggtcgg atcgcctcgc gctcgaaaga cccggcggtg atggccaggg cctgatcctt
     2341 cttggtcgag acggcgaaat ggttggccca gaactgcgcc atgcgctcgt tgaagccgat
     2401 gaccgggctt ttcaccgtgc cgtcgaagcg cgccgcgatc tcggccaacg cggcgggccg
     2461 gatcggattg tcgtcgcggc cgggtttttt cgggccgttg gcgtcgcccg cgttcgccgc
     2521 ctgccgcgct tgccgctttt ccttctggta ggcgtaaagc gcggaaagca ggttgggggt
     2581 ggcgtcgaga ttttgcggct gcggcggtcc ctgcgcgatt tccgactgaa gcacggcgcg
     2641 cgcgtcgccg acggcgccga cgccccgcgc ggtcgcgccg aggccgaagc gcgacagggc
     2701 gatcgccggg tcgtaaaggg tttgcgccat ttcaaacgtc tccgcgcctt gccccccgtc
     2761 agcaggttcg gaaacttgta cggcggattg atggcgtctc cgtcgcggtt aaaacgcttc
     2821 ggagacgatt tccgcgtcgc cggagaacca ggcgcgcttc atgccctgcg aacggaacgg
     2881 ggcgcagatc ccgccgcgcg catcgaccgc gatcagcccg ccttcgccgc ccatgctcgc
     2941 gatgcgcgcc agcacggcgt cggcggccgc gttcagcgcc tcgcccgccg catggcggaa
     3001 ggcgacctgc gcggcggcgt tcgcgcgaat gaaatattcg ccctgaccgg tgcaggagac
     3061 ggcggcgccg acgtcggccc atgtcccggc gccgatcagc ggcgtgtcgc cgacccggcc
     3121 cggcagcttg ccgaacacgc ccgccgtcga ggtggcggcg gccagccgtc catgcgcgtc
     3181 gcggcacacg cagccgaccg tgccgtgcgc cagcgcgccc ggcggatgat tggactcaaa
     3241 gcgccccgcg cgggtgaaat aggcgtcggg atcggccagc gcctcaaaac cctgcccggc
     3301 ggcgaaccgg gcggcgccgt ccccggccag caggacgtgc ggcgtgcgct ccatcaccgc
     3361 gcgcgcgacg tggacccggc tggcgtagcc ttgcagggcg gcgaccgcgc cgcacctctg
     3421 gtcgtggccg gtcatcaccg ccgcgtcgag ttcgtaaacg cccgcggcgt tgggcgaggc
     3481 gcccctgccc gcgacataga gaccggacgc ctccagc
//
LOCUS       DASVLL010000703         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_320029,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000703 DASVLL010000000
VERSION     DASVLL010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320029"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..551
                     /locus_tag="VF402_11535"
     CDS             180..551
                     /locus_tag="VF402_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800977.1"
                     /translation="MNILVPAALAAVMLATAPAQAADCAVRSAHRPVAHRTVHRAPHK
                     PLMKKAVKKTAIRRAEPVCGCGAPPRDPVRHPVILRPDGPYFIDGIPHARRVEVRETA
                     MTPMRRGHDMTRHRHHRHPRR"
     gene            complement(574..2409)
                     /gene="glmS"
                     /locus_tag="VF402_11540"
     CDS             complement(574..2409)
                     /gene="glmS"
                     /locus_tag="VF402_11540"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7800978.1"
                     /translation="MCGIIGIVGREPVAPRLLESLRRLEYRGYDSAGIAVATAEGVAR
                     RRAEGKIRNLEAVIHDEPIEDHVGIGHTRWATHGAPSVRNAHPHACGAVTLVHNGIIE
                     NYAELKAELQARGHVFESDTDTEVIAHLLDEALATAAPRAAFQSVLDRLHGAYALAIL
                     IRGEDETIFGARRGSPLVVGWGEGEMFLGSDALAVGPFTQSVTYLEEGDWVVITRNGA
                     EIHEANGDLVHRPVTVVSASAALVEKGSYRHFMEKEVHEQPESTQRTLSAYLDPVAGA
                     VRADIKAGIDFAQVARVQIIACGTAYYAGAIARYAFEKLAGLPVDVEVASEFRYRDPA
                     MTAGTLAVAVSQSGETADTLAALRWCKAQGLKTAALVNVHTSSMAREADVLWPTHAGP
                     EIGVASTKAFTAQLSALLALAVAAAAQRGRIAPADEAFYVQQLLEAPGLIAEALHMDP
                     DVARVTYELSKARDVIYLGRGGMFPLALEGALKLKEISYIHAEGYAAGELKHGPIALI
                     DENTPVVVIAPDDELFEKTASNVQEVAARGGRVVMITDAPDKAPTLSGDAAQALKIIK
                     APACHGFIAPLIYAVPIQLLAYHTAVHKGTDVDQPRNLAKSVTVE"
     gene            complement(2513..>2726)
                     /locus_tag="VF402_11545"
     CDS             complement(2513..>2726)
                     /locus_tag="VF402_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800979.1"
                     /translation="AFCSLSHLVSLMAPYPLPMVTGKIGPSMKICNLFVARRDGAQTN
                     SALICIHCVAIWAESADFSRIQRDGR"
BASE COUNT          445 a          915 c          890 g          476 t
ORIGIN      
        1 tgacgatggc ggttgcggcg tcgacgttgg gcatggcggt ctcctttgcg cgcgacgcta
       61 cgcttttgcg atttaattgg caacctaaaa gttgcctttt attcgcgcgg gaggtgaagg
      121 cgggtgaatc gtgcgctcaa ggcgcgctca aataccgccc ttccgtaagg agacgcgcca
      181 tgaacatcct cgtccccgcc gcgctggccg ccgtcatgct ggcgacggcg cccgctcagg
      241 ccgccgactg cgccgtcagg tccgcccaca ggcctgtcgc gcacaggacg gtccataggg
      301 cgccccataa gccgctgatg aagaaggccg tgaaaaagac cgcgatccgc agggccgaac
      361 ccgtttgcgg ctgcggcgct cccccgcgcg atccggtgcg tcatccggtc attctgcgcc
      421 cggacggccc gtattttatc gacggcatac cccatgcgcg ccgcgtcgag gttcgcgaga
      481 cggcgatgac gccgatgcgg cgcgggcatg acatgacgcg ccatcgccac catcggcatc
      541 cgcgccggta agcgctttgc gtcttgagcg cggttactcc accgtcaccg atttggcgag
      601 gttgcgcggc tgatcgacgt cggtgccttt gtggacggcg gtgtggtagg cgagaagctg
      661 gatcggcacg gcgtagatca gcggcgcgat gaagccgtgg caggccgggg ccttgatgat
      721 cttcagcgcc tgcgccgcgt cgccggagag ggtcggcgcc ttgtccggcg cgtcggtgat
      781 catcaccacg cggccgccgc gcgcggcgac ctcctgcacg ttggaggcgg ttttctcgaa
      841 cagttcgtcg tcgggggcga tcaccaccac cggcgtgttt tcgtcgatca gcgcgatcgg
      901 cccgtgcttg agttcgcccg ccgcatagcc ctcggcgtgg atatagctga tctccttcag
      961 cttgagcgcg ccttccagcg ccagcgggaa catgccgccg cgccccagat agatgacgtc
     1021 gcgcgccttc gacagctcat aggtcacgcg cgccacgtcc gggtccatat gcagcgcctc
     1081 ggcgatcagg ccgggcgctt cgagcagttg ctgcacgtaa aaggcttcgt ccgccggggc
     1141 gatgcgcccg cgctgcgccg ccgccgccac ggccagggcc agcagggccg acagttgcgc
     1201 ggtgaacgcc ttggtcgagg cgacgccgat ctccggcccg gcgtgggtcg gccacagcac
     1261 gtcggcctcg cgcgccatgc ttgaggtgtg gacattgacg agcgccgccg tcttcagccc
     1321 ctgcgccttg caccagcgca gcgccgccag cgtgtccgcc gtctcgcccg actgcgacac
     1381 cgcgaccgcc aatgtccccg ccgtcatggc cggatcgcgg tagcggaatt ccgaggcgac
     1441 ctcgacatcg accggcaggc ccgccagctt ctcgaacgca tagcgcgcga tggcgcccgc
     1501 ataataggcg gtgccgcagg cgatgatctg cacgcgcgcg acctgcgcga agtcgatccc
     1561 ggccttgatg tcggcgcgca ccgcgcccgc caccggatcg agataggccg acagggtgcg
     1621 ctgcgtgctt tcgggctgct catgcacctc tttttccatg aagtgccggt acgacccctt
     1681 ttcgaccagc gccgccgagg ccgacaccac ggtcaccgga cggtggacca gatcgccgtt
     1741 cgcctcatgg atttcggcgc cgttgcgggt gatcaccacc cagtcgcctt cttcgagata
     1801 ggtgacgctc tgcgtgaacg gcccgaccgc cagcgcgtcg gagccgagga acatttcgcc
     1861 ctcaccccag ccgaccacca gcggcgagcc gcgccgcgcg ccgaagatcg tctcgtcctc
     1921 gccgcggatc aggatggcca gcgcataggc gccgtgcagc cggtccagca cggactggaa
     1981 cgcggcgcgc ggggccgccg tcgcgagcgc ttcgtcgagc aggtgggcga tgacctcggt
     2041 gtcggtgtcg gactcgaaga cgtggcccct ggcctgcaat tccgccttca gttcggcgta
     2101 gttctcgatg atgccgttat ggaccagcgt gaccgcgcca caggcgtgcg gatgggcgtt
     2161 gcgcacgctc ggcgcgccgt gggtggccca gcgggtgtgg cctatgccga catggtcctc
     2221 tatgggctca tcgtggatga cggcttccag attgcggatc ttgccctcgg cgcggcggcg
     2281 cgcgacgcct tcggcggtcg cgaccgcgat cccggcggaa tcgtagccgc gatattcaag
     2341 ccggcgcaaa gactccagca ggcgcggcgc gacgggttcg cgaccgacaa taccgatgat
     2401 tccgcacatg ctgacgtctc cttcgacccg gcgcaagcgc ctatttgcgc ctgatgaata
     2461 gcataccaat ttttcaattg ctataaattc aggtaacatt tcatgacgaa tcttaccggc
     2521 cgtcgcgctg aatacggctg aaatcggcgg attcggccca aattgcgaca caatgaatgc
     2581 aaattaacgc cgaattggtc tgggcgccgt cccggcgcgc aacgaaaaga ttgcaaatct
     2641 tcatcgatgg gcctattttg cccgtcacca taggcaaggg ataaggcgcc atgagcgaaa
     2701 ccaagtggga caggctgcaa aacgcg
//
LOCUS       DASVLL010000704         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_372588,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000704 DASVLL010000000
VERSION     DASVLL010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372588"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF402_11550"
     CDS             <1..228
                     /locus_tag="VF402_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7800980.1"
                     /translation="ESFWRGLFPQRQAAAPQGSAPAPAPAAEPVRDEADRYVPAPAKG
                     GASPAARTEAQPAMEAEDDLEIPSFLRRLAN"
     gene            379..1272
                     /gene="lpxC"
                     /locus_tag="VF402_11555"
     CDS             379..1272
                     /gene="lpxC"
                     /locus_tag="VF402_11555"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7800981.1"
                     /translation="MTPDQNEQTLANAAIIAGTGLHTGVTVRMAVRPAPAGTGVVFVR
                     SDVADRDNRVPALAHNVTKTTLGTVITNAAGVSVSTIEHVMAAFAALSIDNVVVELDG
                     PEVPIMDGSAEPFVQLLDRAGFRRQTTPQSFIEILAPVEVTEGDKRAALLPADRFEVR
                     FEIDFPGTPIGHQSVDLAVTEQSFRDELAAARTFGFLEGVEALRAAGLARGGSLDNAI
                     VIDGDRIMNEGGLRFADEFARHKALDAIGDLYVLGMPILGRFEGIRAGHGLNNALVRE
                     LLSRPDAYRVVTRGPVFAQAG"
     gene            1456..>1792
                     /gene="bamD"
                     /locus_tag="VF402_11560"
     CDS             1456..>1792
                     /gene="bamD"
                     /locus_tag="VF402_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7800982.1"
                     /translation="MLLATASVAGLTACAGKGKQEHLVYEERPVELLYNTGMENLDQK
                     DWSDAILYFQEVQRQHPYSEWSRRAIVMSIYANYQDDKYDDASAAADQFIKLYPGSDL
                     TPYAYYMKAI"
BASE COUNT          325 a          598 c          564 g          305 t
ORIGIN      
        1 gaatcgttct ggcgcggcct gttcccgcaa cgtcaggccg ccgcgccgca aggctcggcc
       61 ccggcgcctg cgcccgccgc cgaacctgtc cgcgacgaag ccgaccgcta cgttccggcc
      121 ccggccaagg gcggcgctag cccggcggcg cgcaccgagg cgcagcccgc catggaagcc
      181 gaggatgatc ttgaaattcc ttcgtttctg aggcgtctgg ccaattaatt ccttattagg
      241 caagacgtta caaacggaaa tacaagttcg tttgtaggac ggcgtcacaa aatctataca
      301 gaatgcgggc cagagatgcg cccgcatttt tgtttgtgcg cgcctgtcgc gcgcggcaag
      361 cgtaacggaa tgacagtcat gacgcctgat cagaacgaac agaccctggc caatgccgcg
      421 atcatcgccg gcacgggact gcatacgggc gtgacggtgc gcatggcggt gcgtcccgcg
      481 cccgccggaa ccggcgtcgt gttcgtgcgc agcgacgtcg cggaccgcga caaccgcgtg
      541 cccgccctgg cgcacaacgt caccaagacc acgctcggca ccgtcatcac caacgccgcg
      601 ggcgtttcgg tctcgaccat cgaacatgtc atggccgcct tcgccgcgct gagcatcgac
      661 aatgtcgtgg tcgaactgga cgggccggaa gtgccgatca tggacggctc ggccgaaccc
      721 ttcgtgcaac tgctcgaccg ggccggtttc cgccgtcaga cgacgccgca atccttcatc
      781 gagatcctgg ccccggtcga ggtgaccgaa ggcgacaagc gcgcggccct gctgcccgcc
      841 gaccgcttcg aggtgcgctt cgagatcgac ttccccggca cgccgatcgg gcatcagtcc
      901 gtcgatctgg ccgtcaccga acaatccttc cgcgacgaat tggccgcggc gcgcaccttc
      961 ggcttcctgg agggcgtcga ggccctgcgc gcggccggtc tggcgcgcgg cggctcgctg
     1021 gacaacgcca tcgtcatcga cggcgaccgc atcatgaacg aaggcggcct gcgtttcgcc
     1081 gacgaattcg cccgccacaa ggcgctggac gccatcggcg acctgtacgt tctgggcatg
     1141 ccgattcttg gccgctttga aggaatccgc gccggacacg gccttaataa tgcccttgtt
     1201 cgcgaattac tgtcgcgccc ggatgcctac cgcgtcgtga cgcgcgggcc tgtgttcgcg
     1261 caggccgggt aaggtcgcgc cgcgccgccc gcgctcatca tgagcgggcg aattcggttg
     1321 gcggggcgcg gacgctcgtg tagatgtatc ctccaaatcg gttggggccg cccggttaag
     1381 tccgaacgcg acgcgcccgc acaagaggaa gacaccgtga gcgttgagac gtttctgagg
     1441 aaatcggcga tcgtaatgct gctggccacg gcgtccgtag ccgggctgac cgcctgcgcg
     1501 ggcaagggca agcaggagca tctggtttac gaagaacgtc cggtggagct gctctacaac
     1561 accggcatgg agaatctcga ccagaaggac tggtccgacg cgattctgta cttccaggaa
     1621 gtccagcgcc agcatccgta ttcggaatgg tcgcgccgcg ccatcgtcat gtcgatctac
     1681 gccaactatc aggacgacaa gtacgacgac gcctcggcgg ccgccgatca gttcatcaag
     1741 ctgtatccgg gcagcgacct gacgccctac gcctattaca tgaaggcgat ct
//
LOCUS       DASVLL010000705         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_426305,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000705 DASVLL010000000
VERSION     DASVLL010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426305"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF402_11565"
     CDS             <1..210
                     /locus_tag="VF402_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7800983.1"
                     /translation="EDGLDMEESTLYPLLRRLETQGLLVSEWREEDKRRKRFYRLSAA
                     GETLLRRLAHEWRTMNASLEKIIGD"
     gene            370..1497
                     /locus_tag="VF402_11570"
     CDS             370..1497
                     /locus_tag="VF402_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800984.1"
                     /translation="MLEDYLRAVSRLLPRAQREDVVAELRDEILTRIEAREAERGRAL
                     TPDETEQLLREVGHPIVVAARYRNEPQHAVGPALYPYWAFFVRLALVLEIAISAIVFI
                     ARAAAGADPAQAMEQAVVSGLTGAMTLIGFATAAAWIIERKAVTIGYVNTWSVRDLRF
                     LDFALWDWSDVSDLLAGRRPGPDRRPSGAAYRHDPDWRLRQSSAASGVAAIVCGVVCI
                     LWWLGLISFGLRPVPVDFGALDIDPGRLAQVDWPGLKAVLYWPVVIYCGALILFGGAM
                     LAWPRGVRLRGALDIALGMAGMGLALWLWTTSPLAPILQPGPASELIGRVLDFFRHPL
                     PVPLELAATLVVLISAVGAAFRTLGGLWELLTGASVRRAGF"
     gene            1683..>3265
                     /locus_tag="VF402_11575"
     CDS             1683..>3265
                     /locus_tag="VF402_11575"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX7800985.1"
                     /translation="MLTGFIPAPLLCALILVATLILFMSGRLRADAVALCALFAGVVA
                     GVVSPRAALAGFGDPAVVAVAAVMVVGQALQATGIAGWVARALIPEKAAFTVRLGMLL
                     LGACLLSAFMNHVAALVITLPLGAEVARASRRSPGLVLMPLSFATMIGGMTTLIATPP
                     NMILSSIRQSALGQPFGFFTMTPVGLAVCAVGLIYLTFIGWRFLPARKAADMGGDAPW
                     RVMELPLDARRVLAREDVARQLRKARTRILALLRNGKAIRWPRDRRLKSGDKLLLLSR
                     ALPAEVREAVDFNRGKESAAPGHVTAGLMVTHGSPLVGHTHEAVEARSDGALQVVAAG
                     PRAANLRCPLDAVAIESGDQLFVSGPPAAIAAFAANQRLIEIDRRDSIPVDLRKAALT
                     LGLFACAIALVLIFRVPPAVSFLGAAALLTAFRLISVEDAYGAVNWSIIVLLAAMIPL
                     GASFESTGAAAFAADYVGGLLRHAPLITAVAAVCALTLGLSILIHNAPAAIIMGPVAL
                     KIAALLHAPPDALLLAVLVG"
BASE COUNT          443 a         1120 c         1136 g          566 t
ORIGIN      
        1 gaggacgggc tggatatgga ggaaagcacg ctctacccgc tgctgcgccg cctggagacg
       61 cagggcctgc tggtcagcga gtggcgcgag gaggacaagc gcaggaagcg tttttaccgg
      121 ctgtcggcgg cgggcgaaac cctgctgcgg cggctggcgc atgagtggcg gaccatgaac
      181 gcctcactcg aaaagatcat cggcgattag agcgctttcc gaaaagtgtg acgcactttt
      241 cggattaaaa agcgcgtaaa agcataaagt tagagcgttt ccccaaaaag tggctccact
      301 ttttggatgc ggaagagcga caaaacaaag acttggagtc ccaaaggacg ggcgaggttc
      361 gaaatggaca tgctggaaga ttatctgcgc gcggtttcgc gcctgctgcc cagggcgcag
      421 cgcgaagacg tcgtcgccga actgcgcgac gagatcctga cgcggatcga ggcgcgcgag
      481 gccgaacgcg gccgcgccct cacgccggac gagaccgaac agttgctgcg cgaggtcggc
      541 cacccgatcg tggtcgccgc gcgctatcgc aacgagccgc agcacgccgt cggcccggcc
      601 ctctatccct actgggcctt tttcgtgcgg ctggcgctcg tgctggaaat cgccatctcc
      661 gccatcgttt tcatcgcgcg cgccgccgcc ggcgccgatc cggcgcaggc gatggaacag
      721 gccgtggttt cgggtctgac cggggcgatg accctgatcg gcttcgccac cgccgcggcc
      781 tggatcatcg agcgcaaggc cgtcaccatc ggttacgtca acacctggag cgtgcgcgac
      841 ctgcgcttcc tcgacttcgc cctgtgggac tggagcgatg tcagcgacct gctggcgggc
      901 cgccgccccg gaccggaccg caggccgtcc ggcgcggcgt atcgtcacga tcccgactgg
      961 cgcctgcgtc agtcctccgc cgccagcggc gtcgccgcca tcgtgtgcgg ggtcgtctgc
     1021 atcctgtggt ggctgggcct gatcagcttc ggcctgagac cggttccagt cgatttcggc
     1081 gcgctcgaca tcgatcccgg ccgtctggcg caggtcgact ggccgggcct gaaggccgtc
     1141 ctgtactggc cggtcgtgat ctattgcggc gcgctgatcc tgttcggcgg ggccatgctg
     1201 gcctggccgc gcggcgtgcg cctgcgcggc gcgctcgaca tcgcgctcgg catggcgggc
     1261 atggggctgg ccctgtggct gtggacgacc tcgccgctgg ccccgatcct tcagccgggg
     1321 ccggcgtccg aactgatcgg gcgcgtgctg gactttttca ggcatccgct gcccgtgccg
     1381 ctggagctgg ccgccacgct cgtcgtcctg atctccgccg tcggcgccgc ctttcgcacc
     1441 ctcggcggcc tgtgggagct gctgaccggc gcgtccgtgc ggcgcgcggg cttctagagc
     1501 gtttccccaa aaagcggttc cactttttgg ataaggaaga gcgacaaaac aaagatttag
     1561 agcgccgatc tgattctatc agatcgaaaa gcactctagg gccaaaagcg gcgacattcc
     1621 ccatcgaatt tttagcggcg catcccgaat ggtgaagcct caaccatgcc gggggcgatc
     1681 gcttgctgac gggcttcatt cctgcgccgc ttctgtgcgc gcttattctg gtcgccaccc
     1741 tgatcctgtt catgagcggc aggctgcggg ccgacgcggt cgccctgtgc gccctgttcg
     1801 cgggcgtcgt cgcgggcgtg gtgtcgccca gggccgctct ggccgggttc ggcgatccgg
     1861 cggtcgtggc ggtcgccgcc gtcatggtgg tgggtcaggc gctgcaggcg accgggatcg
     1921 cgggctgggt ggcgcgcgcc ctgatcccgg aaaaggccgc cttcacggtg cggctgggca
     1981 tgttgctgct gggcgcgtgc ctgttgtcgg ccttcatgaa ccatgtcgcc gcgctggtca
     2041 tcaccctgcc tctgggcgcc gaggtcgcgc gcgccagccg cagatcgccc ggcctcgtgc
     2101 tgatgccgct gtccttcgcg accatgatcg gcggcatgac caccctgatc gcgacgccgc
     2161 cgaacatgat cctgtcctcg atccgccaga gcgcgctggg ccagccgttc ggcttcttca
     2221 ccatgacgcc ggtcgggctg gccgtgtgcg cggtcgggct gatctacctg accttcatcg
     2281 gctggcgctt cctgcccgcg cgcaaggccg ccgacatggg cggcgacgcg ccgtggcggg
     2341 tgatggagct gccgctggac gcgcgccgcg tgctggcgcg cgaggacgtg gcgcgtcagt
     2401 tgcgcaaggc gcgcacgcgc atcctggcgc ttttgcgcaa cggcaaggcg atccgctggc
     2461 cgcgcgaccg gcggctgaaa tcgggcgaca agctgctcct gttgtcgcgc gccctgcccg
     2521 ccgaggtgcg cgaagcggtc gacttcaacc gcggcaagga aagcgccgcg ccgggccacg
     2581 tcacggcggg cctgatggtc acgcacggct cgccgctggt cggccatacc cacgaggcgg
     2641 tcgaggcgcg cagcgacggc gccttgcagg tcgtcgccgc cgggccgcgg gcggcgaacc
     2701 tgcgctgccc gctcgacgcc gtggcgatcg agagcggcga tcagttgttc gtgtccgggc
     2761 cgcccgccgc catcgcggcc ttcgccgcca atcagcgcct gatcgagatc gaccggcgcg
     2821 actcgatccc ggtcgacctg cgcaaggcgg cgctgacgct cggcctgttc gcctgcgcca
     2881 tcgcgctggt gctgatcttc cgcgtgccgc ccgccgtcag ctttctgggc gcggcggcgc
     2941 tgctcacggc cttccgcctg atcagcgtcg aggacgccta cggcgcggtc aactggagca
     3001 tcatcgtgct gctggccgcc atgatcccgc tgggcgccag cttcgaaagc accggcgcgg
     3061 cggccttcgc cgccgattat gtcggcgggc tgctgcgcca cgcgccgctg atcacggccg
     3121 tcgccgccgt gtgcgcgctg acgctgggcc tgtcgatcct gatccacaac gcgcccgccg
     3181 ccatcatcat ggggccggtc gcgctgaaga tcgcggcgct gctgcacgcg ccgcccgacg
     3241 cgctgctgct ggccgtgctg gtcgg
//
LOCUS       DASVLL010000706         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_47856, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000706 DASVLL010000000
VERSION     DASVLL010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47856"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..586)
                     /locus_tag="VF402_11580"
     CDS             complement(26..586)
                     /locus_tag="VF402_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX7800986.1"
                     /translation="MRLHRFAAPAALAAVGAVTAAVVSARAAMPVGNRAVPGPAKPVD
                     IAAYAGLWYEIGRYDNRFERGCEAVTAEYALRDDGKVSVINRCRAGGVEGRVRTAHGV
                     AKIVPDSGGAQLKVSFFGPFYFGDYWVLDHAHDYGWSIVGEPSGRYLWLLSRSAHPDA
                     ATRAMIETRVRQLGYDTGLIRPTLQP"
     gene            complement(684..1028)
                     /locus_tag="VF402_11585"
     CDS             complement(684..1028)
                     /locus_tag="VF402_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX7800987.1"
                     /translation="MLRITLLAGAALLVWAGAAAAAQPIEGDWLTLKGDTARIAPCEG
                     DKFCITLIDGQYAGKQIGVLKGMGGQYIGQVTKPQNGKTYDGSATVTGQALKLQGCAL
                     KIFCESQVWTRK"
     gene            1241..2551
                     /locus_tag="VF402_11590"
     CDS             1241..2551
                     /locus_tag="VF402_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX7800988.1"
                     /translation="MTAIKTAASAFALALPLAAGMALAGAAHAAGFYLQDLSAKQAGN
                     AYSGAATAQGADALWWNPAAIAGTTTRELSFGAAYINPKGSVNDTGSVIVRPGQAPAG
                     MGGDAAADGPIKNGVLPNVAFAMPLTDRLAFGVSVTSPFSFETNYATDSWARYNATKT
                     RLTTVDIQPTLAFKVNDWLDLGLGLNAEYTKATLGNQMPNLSAALPDGSLLLEGDGWD
                     TGFSAGARAHTGNLVFGLSYKSAVKHQLDGTVNITGLLGPLATANLSQGGVRATFSTP
                     SQLILGASVQATPQLALHAQVVQFGWSKFDDITLGAPLNEAMPEDYRDTTSVAVGLDY
                     AINRQWTVRAGVQTDPTPTRDDHRDARVPDSDRVDYAIGTSWQVTPTFALDAAYTYVA
                     FDKSRLNYTTAAYYGTAAQTNMVINGAMDDAKANVLIVGGRLSF"
     gene            complement(2633..>3436)
                     /locus_tag="VF402_11595"
     CDS             complement(2633..>3436)
                     /locus_tag="VF402_11595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7800989.1"
                     /translation="IVIDTTKGRIVVELYPNLAPLSSERLITLTRQHFYDGLVFHRVI
                     EDFMDQTGDPKGDGTGGSTLPDVKAEFSIRRGPDFPFAIAARPTGSLIGFVGAMPVES
                     QVNELFPMSKDGKVDLWPQFCQGVVGMAHSYDPDSNNSQFFLMRGANSSLNKGYTAVG
                     VVIQGLDVVRKIKIGEPPVDPDKMTKVQVLADMPAADRPNLEIMDTSAPQFQAVIDAV
                     RLDKGADFSACDVKIPVKDLNAPAPAAETAATPAPAPAKAAKAKGKKKK"
BASE COUNT          582 a         1192 c         1090 g          572 t
ORIGIN      
        1 atcttcgccg gcgacggcat ctgatctagg gctgcaaggt cggccggatc agccccgtat
       61 cgtaccccag ttgccgcacg cgcgtctcga tcatggcgcg cgtcgcggcg tcggggtgcg
      121 cgctgcggct caggagccac agataccgcc ccgacggctc gccgacgatt gaccagccat
      181 agtcgtgcgc atggtccagc acccagtagt cgccgaaata gaacggcccg aagaacgaca
      241 ccttcaactg cgccccgccg ctgtccggca cgatcttcgc cacgccgtgc gcggtcctga
      301 cgcgcccctc gacgccgccg gcgcggcagc ggttgatcac gctgaccttg ccgtcgtcgc
      361 gcagcgcgta ttcggcggtc accgcctcgc agccgcgctc gaagcggttg tcatagcgtc
      421 cgatttcata ccacagaccg gcataggcgg cgatgtcgac cggcttggcg ggtccgggca
      481 cggcgcggtt gccgaccggc atggcggcgc gcgcgctgac gaccgccgcc gtgacggcgc
      541 cgacggcggc cagggccgcg ggcgcggcga aacgatgaag acgcatgagg cttctcccga
      601 aaatccaacg ccatcgggat gccgcaaagc ggataaggat tcaattcagg gttaggccag
      661 ggcgaggcca aggcgaggcc agcctatttg cgcgtccaga cctgcgactc gcaaaagatt
      721 ttcagcgcgc agccctgtag cttgagcgcc tgaccggtga ccgtggccga gccgtcatag
      781 gtcttgccgt tctggggctt cgtcacctga ccgatatatt gcccgcccat gcctttcagc
      841 acgccgatct gcttgcccgc gtattggccg tcgatgaggg tgatgcagaa cttgtcgccc
      901 tcgcacggcg cgatccgggc cgtgtcgcct ttcagcgtca gccagtcgcc ttcgatcggt
      961 tgcgccgcgg cggcggcgcc cgcccagaca agtaaggcgg cgcccgccag cagggtgata
     1021 cgcagcattt tcagcctcct gttccggcgt ctgttcccgg cgcctgtggc gcaagagtaa
     1081 cgaagcttga cgataacgta aaataaattc atcgaattga cgcgcacgtc aacaaaaaac
     1141 ttgacgcccc ggtcaagtgt gagatcatcc gcgttgacgc caacgtaaag agcgtcgcga
     1201 aaacacccat caggtggact tgccccccag gagcgccttt atgaccgcca tcaaaaccgc
     1261 cgcctcggcg ttcgccctcg ccctgccgct cgccgcgggc atggcccttg ccggcgcggc
     1321 ccacgccgcc ggtttctatt tgcaggatct gtccgcgaag caggcgggca acgcctattc
     1381 cggcgcggcc acggcgcagg gcgcggacgc cctgtggtgg aatcccgccg ccatcgccgg
     1441 aaccaccacc cgggagctga gcttcggcgc cgcctatatc aacccgaaag gctcggtgaa
     1501 cgacaccggt tcggtgatcg tgcgtccggg gcaggcgccc gccggcatgg gcggcgacgc
     1561 tgccgccgac ggcccgatca agaacggcgt gctgccgaac gtcgcgttcg ccatgccgct
     1621 caccgaccgc ctcgccttcg gcgtttcggt gacctcgccg ttcagcttcg agaccaatta
     1681 cgccaccgac agctgggcgc gctacaacgc caccaagacc cgcctgacca cggtggacat
     1741 ccagccgacc ctggccttca aggtcaatga ctggctcgat ctcggcctcg gtctcaacgc
     1801 ggaatacacc aaggcgaccc tgggcaacca gatgccgaac ctgagcgcgg ccctgcccga
     1861 cggcagcctg ctgctggaag gcgacggctg ggacaccggc ttttcagccg gcgcgcgggc
     1921 gcacaccggc aatctcgtct tcggcctgag ctacaaatcc gccgtcaagc accaactgga
     1981 cggcacggtc aacatcaccg gcctgctggg gccgctggcc accgccaacc tgtcgcaggg
     2041 cggcgtccgg gcgaccttct cgacgccgtc gcaactgatc ctgggcgcct cggtgcaggc
     2101 gacgccgcaa ctggccctgc acgcgcaggt cgttcagttc ggctggagca agttcgacga
     2161 catcacgctc ggcgcgccgc tgaacgaagc catgccggaa gattatcgcg acaccacctc
     2221 ggtggcggtc ggcctcgact atgcgatcaa ccggcaatgg acggtccgcg cgggcgtgca
     2281 gaccgacccg accccgaccc gcgacgatca ccgcgacgcg cgcgtgcccg attccgaccg
     2341 cgtcgactac gccatcggca cgagttggca ggtcacgccg accttcgcgc tcgacgcggc
     2401 ctatacctat gtcgccttcg acaagagccg gctgaactac accaccgccg cctattacgg
     2461 cacggcggcg cagaccaata tggtcatcaa cggcgccatg gacgacgcca aggccaatgt
     2521 gctgatcgtc ggcggccgcc tgagcttctg atccgccatc ggtgttttgc aaaaaagccg
     2581 ccgccgaatc gttcggcggc ggctttgtca tgcggccggg cgggccttgc ggttactttt
     2641 tcttcttgcc cttcgccttg gcggccttcg ccggagcggg cgcgggcgtc gccgccgttt
     2701 cagcggcggg cgcgggggcg ttcagatcct tgaccggaat cttgacgtcg caggccgaaa
     2761 agtccgcgcc cttatccagc cgcacggcgt cgatcaccgc ctgaaactgc ggcgcggagg
     2821 tgtccatgat ttcgaggttc gggcggtcgg cggcgggcat gtcggccagc acctgcacct
     2881 tggtcatctt gtcgggatcg accggcggct cgccgatctt gatcttgcgc acgacgtcca
     2941 gcccctgaat gaccacgccg acggcggtat agcccttgtt cagcgacgaa ttggcgccgc
     3001 gcatcaggaa gaactggctg ttgttgctgt ccgggtcata gctgtgcgcc atgccgacca
     3061 cgccctgaca gaattgcggc cacagatcga ccttgccgtc cttcgacatc gggaacagct
     3121 cgttgacctg gctttccacc ggcatggcgc cgacgaagcc gatcagcgag ccggtcggcc
     3181 gcgccgcgat ggcgaacggg aagtccggcc cgcgccggat gctgaactcg gccttcacgt
     3241 ccggcagggt cgagccgccc gtgccgtcgc ccttggggtc gccggtctgg tccatgaagt
     3301 cttcgatgac gcggtgaaag acgaggccgt catagaaatg ctggcgcgtc agcgtgatca
     3361 gccgctcgct ggatagcggc gccaggttcg gatacagctc gacgacgatg cggcccttgg
     3421 tggtgtcgat gacaat
//
LOCUS       DASVLL010000707         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_480834,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000707 DASVLL010000000
VERSION     DASVLL010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480834"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VF402_11600"
     CDS             <1..679
                     /locus_tag="VF402_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7800990.1"
                     /translation="QFYRFALAENLVAADPSRKVAAARKGRSLPKILSRDEIEALIRA
                     ADEKDAHQAVRLECLIEMAYASGMRISELVGLRLDAVARDPAYLIVKGKGGVERLVPL
                     NPSARTAIRAYLDIRPHFLPRGDKANPYLFASRGRSGHLTRRRVGQLLDEAAANAGID
                     PARVSPHVLRHAFATHLLEGGADLRVVQTLLGHADISTTQIYTHVANERLREVVESHH
                     PLAKRAH"
     gene            734..925
                     /locus_tag="VF402_11605"
     CDS             734..925
                     /locus_tag="VF402_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX7800991.1"
                     /translation="MRTTLTLDDDLLDEAVALTGVTERAVLMREALKALIERESARRL
                     ARLGGTEPDLVAPPRRTFG"
     gene            934..1293
                     /locus_tag="VF402_11610"
     CDS             934..1293
                     /locus_tag="VF402_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7800992.1"
                     /translation="MILADTSIWIAHLRNERGELALRLERGQILAHPFVLGELMLGGL
                     GRRSPVPHLLRNLPQAVCASHDEVLSLIGRANLSGRGVGWVDAHLLAACRLTPDARLW
                     TLDRKLHDLAHELDVVL"
     gene            1385..>1889
                     /locus_tag="VF402_11615"
     CDS             1385..>1889
                     /locus_tag="VF402_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7800993.1"
                     /translation="MRHYLEFERPIADLEAKIEELSALSESGGARLDKELEALRLRTD
                     SLRRETYSRLEPWQKTQVARHPERPHLIDYIAGLIEDFVELRGDRKFGDDQAIVGGLG
                     RFRGQAVVVMGHEKGHDTNTRLKHNFGMARPEGYRKAVRLMEMAEQFGLPVLSFVDTA
                     GAYPGLDA"
BASE COUNT          302 a          621 c          657 g          309 t
ORIGIN      
        1 gcagttctat cgcttcgcgc tggcggaaaa cctcgtcgcc gccgatccga gccgcaaggt
       61 ggccgccgcg cgcaaggggc ggtcgctgcc gaaaatcctg tcgcgcgacg agatcgaggc
      121 gctgatccgc gcggcggacg agaaggacgc gcatcaggcc gtgcggctgg aatgtctgat
      181 cgagatggcc tatgcgtcgg ggatgcggat ttcggaactg gtcgggctgc ggctcgacgc
      241 ggtggcgcgc gatccggcct atctgatcgt caagggcaag ggcggggtcg agcggctggt
      301 gccgctcaac ccgtcggcgc ggacggcgat ccgggcctat ctcgacatcc gcccgcactt
      361 cctgccgcgc ggggacaagg ccaatcccta tctgttcgcc tcgcgcggcc ggtccggtca
      421 cctgacccgc cgccgggtcg ggcagttgct ggacgaggcg gcggcgaacg cgggcatcga
      481 cccggcgcgc gtcagcccgc acgtgctgcg ccacgccttc gccacgcacc tgctggaagg
      541 cggggccgac ctgcgcgtcg tgcagaccct gctgggccac gccgacatct ccaccacgca
      601 gatctacacc cacgtcgcga acgagcgcct gcgcgaagtg gtcgaaagcc accatccgct
      661 ggcgaagcgc gcgcattagg catgcattga aatccgcatc gcttgatgct atttctgatg
      721 caggagatgt ctcatgcgca caaccctgac cctcgatgac gatctgctgg acgaagccgt
      781 cgccctgacc ggcgtgacgg agcgcgccgt gctgatgcgc gaggcgttga aggcgctgat
      841 cgaacgcgaa agcgcgcggc ggctggcgcg tctgggaggg accgagccgg atctggtcgc
      901 gccgccgcgc cgcacgttcg gctgacctcc catatgattc tggccgatac gtcgatctgg
      961 atcgcgcact tgcgcaacga gcgcggcgaa ctggccctgc ggctggagcg cggccagatt
     1021 ctcgcccatc cctttgtgct gggggagttg atgctgggcg gcctggggcg gcgcagtccc
     1081 gtgccgcatc ttctgcgcaa cctgccgcag gcggtctgcg ccagccacga cgaggtgctg
     1141 agcctgatcg ggcgcgcgaa tctgtccggc cgcggagtcg gctgggtcga cgcgcacctg
     1201 ctggcggcct gccgcctgac gccggacgcc cggctgtgga ctctggaccg gaaactgcac
     1261 gatctggcgc acgagctgga cgtggtcttg tagaaaaacc cgcattaagg gacggggggt
     1321 tgctgtcggg cgccttctcc cttacatgtg cgcgctttaa aggaaggctg ttcggaacat
     1381 cgccatgcgt cattatctgg aattcgagcg tcccatcgcg gatctggaag ccaagatcga
     1441 agagctgtcg gccttgtccg aatcgggcgg ggccaggctc gacaaggagc tggaggcgct
     1501 gcgcctgcgc accgattccc tgcgccgcga gacctattcg cgcctcgaac cgtggcagaa
     1561 gactcaggtc gcgcgccatc ccgaacgtcc gcacctgatc gactacatcg ccgggctgat
     1621 cgaggacttc gtcgagttgc gcggcgaccg caagttcggc gacgatcagg ccatcgtcgg
     1681 cggcctcggc cggtttcgcg gtcaggcggt ggtggtcatg ggccacgaaa agggccacga
     1741 caccaatacc cgcctgaagc acaatttcgg catggcccgc ccggaaggct atcgcaaggc
     1801 ggtgcgcctg atggagatgg ccgagcagtt cggcctgccg gtgctgagct tcgtcgacac
     1861 ggcgggcgcc tatccgggcc tcgacgccg
//
LOCUS       DASVLL010000708         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_48063, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000708 DASVLL010000000
VERSION     DASVLL010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48063"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VF402_11620"
     CDS             <1..477
                     /locus_tag="VF402_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7800994.1"
                     /translation="SLIGYQGEFLTDTRGSGVLNRVFSHYEGYKGPIEQQRKGVLVSN
                     SDGETAAYALWNLEERGTMFVGAGEKTYMGMLIGENSRSDDLDVNPMKAKQLTNVRAS
                     GKDEAVRLTPPRRLTLEQAIAYILDDELVEVTPKNIRLRKKELNPSFRKKRVKEDA"
     gene            complement(539..1927)
                     /locus_tag="VF402_11625"
     CDS             complement(539..1927)
                     /locus_tag="VF402_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7800995.1"
                     /translation="MAGSLHGFDHLGRERLAAEVLDNKISYGMIAVALQYRAAPGAPP
                     QTLRLPKQDNTYYINIHYSEYDNAIYWSEFNYTSGVCSIFRYDIATGQKTLYRTGPNK
                     DMDIAFDKFGHIVGVTTLTDRVHTEWTDPYYLKMIKAVEAFFPKALVDIADITEDGRD
                     IVFVISAPEAPPSYYIYSARTRDLARIGGAYPELDGRTLAPMTYVTYKARDGLDIPAY
                     VTKRRDTPPHAPLIVMPHGGPAMRDLYDFDAWAQFFASKGYVVLQPQYRGSAGFSDAF
                     ERAGDAHLAQMTTDLEDGVHYLEAQGAVDPRKICVVGWSWGGYLAQAALAFTPGTYTC
                     GVSGAGISNLSDSLEHDNDIWWGGYSSAYWHEVIGRDGAAIRAVSPIEHIDSIRAPLL
                     LIHGRLDDNVDVRQSQRMYAAMKAAGKDVTYIEIPDMQHGPDTAEQNLTVLKAMGAFV
                     AKAFAAADKSAH"
     gene            complement(1934..2479)
                     /locus_tag="VF402_11630"
     CDS             complement(1934..2479)
                     /locus_tag="VF402_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7800996.1"
                     /translation="MFGRYLLARLAMAGLIAAAPFSSQAQPQTQAADAPLKAEDFARD
                     PYMSPPQISPDGKALAWVEGNRVVVHNLSDNSEKTQDGGHNRLDSLQWVGNDYLVVYL
                     KDDEVAKSYYLKPVSYSPIVIRRDARYVRLLFAHDGSKATTTAMWPIVSYVDADPPYA
                     VTMQDDFTDIIELPTGRHRSG"
BASE COUNT          468 a          756 c          884 g          470 t
ORIGIN      
        1 tcgctgatcg gctatcaggg cgagttcctg accgacacgc gcggctccgg cgtgctgaac
       61 cgcgtcttct cgcactacga aggctataaa ggcccgatcg agcagcagcg caagggcgtg
      121 ctggtgtcca actcggacgg cgaaacggcg gcctacgcgc tgtggaacct cgaagagcgc
      181 ggcaccatgt tcgtcggcgc gggcgaaaag acctatatgg gcatgctgat cggcgagaat
      241 tcgcgttcgg acgatctcga cgtcaacccg atgaaggcca agcaactgac caacgtgcgc
      301 gcctccggca aggacgaggc cgtgcgcctg accccgccgc gccgcctgac gctggaacag
      361 gcgatcgcct acatcctgga cgacgaactg gtcgaggtca cgccgaagaa catccgcctg
      421 cgcaagaaag aactgaaccc cagcttccgc aagaagcggg tgaaggaaga cgcgtaaagg
      481 aagtcagccc ctttgaagta tagacggctc cggcggtgcg aaccgccggg gccgtttttt
      541 aatgcgccga tttgtcggcg gcggcgaagg ccttggcgac gaaggcgccc atggctttga
      601 gaacggtcag attctgttcc gccgtatcgg ggccgtgctg catgtcgggg atttcgatat
      661 aggtcacatc cttgcccgcc gccttcatgg ccgcgtacat gcgctggctc tggcgcacgt
      721 cgacattgtc gtcgagccgg ccgtggatca gcagcagcgg cgcgcggata gagtcgatat
      781 gctcgatggg cgagacggcg cggatcgcgg cgccgtcgcg tccgatcacc tcatgccagt
      841 aggcgctgga ataaccgccc caccagatgt cgttgtcgtg ctccagcgag tcggacaggt
      901 tggatatgcc cgcgccggac acgccgcagg tataggttcc gggcgtgaag gccagcgcgg
      961 cctgcgccag atagccgccc cacgaccagc cgacgacgca gatcttccgg ggatcgaccg
     1021 cgccctgggc ttcgagataa tggacgccgt cttcgaggtc ggtcgtcatc tgcgccagat
     1081 gggcgtcgcc cgcgcgctcg aacgcgtcgc tgaatccggc ggagccgcga tattgcggtt
     1141 gcagcaccac atagcctttc gaggcgaaga actgcgccca ggcgtcgaaa tcgtagaggt
     1201 cgcgcatggc ggggccgccg tgcggcatga cgatcagcgg cgcgtgcggc ggcgtatccc
     1261 ggcgcttcgt cacataggcc gggatgtcga ggccgtcgcg cgccttgtag gtgacatagg
     1321 tcatcggcgc cagcgtgcgg ccgtcgagtt cgggataggc gccgccgatg cgcgccagat
     1381 ccctggtgcg cgcgctgtag atataatagc tgggcggcgc ttccggcgcg gagattacaa
     1441 aaacgatgtc gcggccgtcc tcggtgatgt cggcgatatc gaccagcgcc ttcgggaaga
     1501 aggcttcgac cgccttgatc atcttgagat agtagggatc ggtccattcg gtgtggacgc
     1561 ggtcggtcag ggtggtgacg ccgacgatat ggccgaactt gtcgaaggcg atgtccatat
     1621 ccttgttggg accggtgcgg tagagggttt tttggccggt cgcgatgtcg tagcggaaga
     1681 tggagcagac gccgctggta tagttgaatt ccgaccagta gatcgcgttg tcgtactcgg
     1741 aataatggat gttgatgtaa taggtgttgt cctgcttggg caggcgcaaa gtctgcggcg
     1801 gggcgccggg cgcggcgcga tactgcaagg cgacggcgat catgccgtag ctgatcttgt
     1861 tgtccagaac ttcggcggcc agacgctccc ggccaagatg gtcgaagccg tgcagcgatc
     1921 ccgccatcag aggctagccc gaccgatgcc gccccgtcgg aagctcgatg atgtcggtga
     1981 agtcgtcctg catggtgacg gcgtagggcg gatcggcgtc gacatagctg acgatcggcc
     2041 acatggcggt ggtcgtggcc ttggagccgt catgcgcgaa cagcaggcgc acatagcgcg
     2101 cgtcgcgcct gatcacgatg ggcgaatagc tgaccggctt gaggtagtag gatttggcga
     2161 cttcgtcgtc cttcagatag accaccagat aatcgttgcc gacccattgc aggctgtcca
     2221 gccggttatg gccgccgtcc tgcgtctttt ccgaattgtc gctcaggtta tggacgacga
     2281 cgcggttgcc ctcgacccag gccagcgcct tgccgtcggg cgatatctgc ggcggactca
     2341 tataggggtc gcgggcgaaa tcctcggctt tgagcggtgc gtcggcggct tgagtttggg
     2401 gctgggcctg agatgagaac ggcgccgccg cgatcaggcc ggccatcgcc aggcgcgcca
     2461 aaagataccg tccgaacatg atttccccct gtgcgggctg cgccgcttac cgggagttta
     2521 aacatggacg gcaggcgctt tcaagagagg ccgcctggcg ggggaaaccg cgcggcta
//
LOCUS       DASVLL010000709         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_211333,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000709 DASVLL010000000
VERSION     DASVLL010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211333"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VF402_11635"
     CDS             complement(<1..660)
                     /locus_tag="VF402_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019564268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7800997.1"
                     /translation="MTQPNAAVRLSPPKNPVGGRIALPGSKSITNRVLLIAALARGTS
                     HISGALKSDDTTYMAQALRQLGVTVTESGAADFVVESTGVLTAAKEPLFLGNAGTAVR
                     FLTAAAALIDGETVLTGDAHMQKRPIAPLVAALNAAGVTASAPSGCPPVTVTSRGGFA
                     NRGVTVDASLSSQYVSALLMAGVAGETPLRLGVAGGDIGARGYIDITLACMRAFGAEI
                     AQ"
     gene            807..1301
                     /locus_tag="VF402_11640"
     CDS             807..1301
                     /locus_tag="VF402_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX7800998.1"
                     /translation="MADPALGAKQVCPNCTAKFYDLNKHPAHCPRCDFEFDQEEIVRT
                     RRTRVRAAEPDYEDTDEEAEDQVKAKTKGEDEDEDEEADVTAPEIDEVVADDPIVIDE
                     DEDLDPADPARVGAGDNVDMDIEDADLDDDDADDVPFLEDDDDDDFDDDIEGLPGDDD
                     EDNI"
     gene            1392..1808
                     /locus_tag="VF402_11645"
     CDS             1392..1808
                     /locus_tag="VF402_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HEX7800999.1"
                     /translation="MTVRLRPHHLLCMLTFSGEGYTPAFVDNFAGVIRRLNDGEPIEV
                     VDGIDDICPPLLSEAFPHCHEYGVTLRDRDAREDLAAVLGQPVQTLRDLPDLAALRRA
                     FAAGDTRRGCQGCEWFGLCSDIAVTGFRTARLGQTP"
BASE COUNT          334 a          613 c          626 g          300 t
ORIGIN      
        1 ctgcgcgatt tccgccccga aggcgcgcat gcaggccagc gtgatgtcga tatagccgcg
       61 cgcgccgatg tcgccgcccg cgacgccgag ccgcagcggc gtctcgcccg cgacgcccgc
      121 catcagcagg gccgacacgt actggctcga caggctggcg tccaccgtga cgccgcggtt
      181 ggcgaagccg ccccggctgg tcacagtcac cggcgggcag ccgctcggcg ccgaggccgt
      241 cacgcccgcc gcgttgagcg ccgccaccag cggcgcaatg ggccgcttct gcatatgcgc
      301 gtcgccggtc agcaccgtct cgccgtcgat cagggcggcg gcggcggtca gaaagcgcac
      361 cgccgtgccc gcattgccga ggaacagggg ctctttcgcc gccgtcagca cgcccgtgct
      421 ttcgacgacg aaatcggcgg cgccgctttc ggtcaccgtc acgcccaact ggcgcagggc
      481 ctgcgccata taggtggtgt cgtcgctttt gagcgcgccg gaaatatggc tggtccccct
      541 ggccagggcc gcgatcagca gcacgcggtt ggtgatggat ttggagccgg gcagggcgat
      601 cctgccgccg acagggtttt tcggcggcga taatctgacg gcggcgttcg gttgggtcat
      661 ggtctggccg ggatgaggcc ccgtctttgc ggtgcaaatc ggcaggcgaa aaattgcgtc
      721 gggggcggat ttaagctttt gacaggaggc ttatctagtg gcaagggggt cgaacgcaac
      781 agctattttc tgccagagga tttatcgtgg ctgatcccgc actaggcgcc aagcaagtct
      841 gcccgaactg cacggcgaaa ttctacgacc tgaacaagca ccccgcgcat tgcccgcgct
      901 gcgacttcga gttcgaccag gaagaaatcg tgcgcacccg ccgcacccgc gtccgcgccg
      961 ccgagccgga ttacgaggat acggacgagg aagccgaaga tcaggtcaag gccaagacca
     1021 agggcgaaga cgaggatgag gacgaggagg ccgatgtcac cgcgcccgaa atcgacgaag
     1081 tggtcgcgga cgatccgatc gtcatcgacg aggacgagga tctcgatccc gccgatccgg
     1141 cgcgcgtcgg cgcgggcgac aatgtcgaca tggacatcga ggacgccgat ctcgacgatg
     1201 acgacgccga cgacgtgccc ttcctcgaag acgacgacga cgacgatttc gacgacgaca
     1261 tcgagggcct gcccggcgat gacgacgagg acaatatcta aaggaagcag ccgccggaac
     1321 gctccggcgg cttttttctt gagcttgcgc gacaaggcgc atagtctggc gcggttccgg
     1381 ggatatcgcc gatgaccgtg cgtttgcggc cgcaccatct gctgtgcatg ctgaccttct
     1441 caggcgaggg ctacacgccc gccttcgtcg ataatttcgc cggggtgatc aggcgcctca
     1501 acgacggcga gccgatcgag gtcgtggacg gaatcgacga catctgcccg ccgctgctga
     1561 gcgaggcctt tccgcactgt catgaatacg gcgtcaccct gcgcgaccgc gacgcgcgcg
     1621 aggatctggc cgccgtgctg ggccagccgg tgcagacgct gcgcgacctg cccgatctgg
     1681 ccgccctgcg gcgcgccttc gccgccggag acacgcggcg cggctgtcag ggctgcgaat
     1741 ggttcggcct gtgcagcgac atcgccgtca ccggatttcg caccgcgcgc ctgggtcaga
     1801 cgccctgaaa cgcccgtttt tgctggcctt ttccgccgcc tttgaaaaaa agcaaaaaat
     1861 cgcgcaaaag ggc
//
LOCUS       DASVLL010000710         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_265619,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000710 DASVLL010000000
VERSION     DASVLL010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265619"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VF402_11650"
     CDS             <1..528
                     /locus_tag="VF402_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX7801000.1"
                     /translation="TPQNIVIGGAAGAFPPVIGWAAATGHAPLEAWVLFAIIFLWTPP
                     HSWALALYTTEDYANAGIPMMPVVRGAKATRLQILLYSLVMAPVACLPIAFGTGGVAY
                     AVVSVLGGAVFIYLAVRVFLSRAGDHPQGGEAGLYDVKKEALAARNLFAFSILYLTLL
                     FATLLAEHAIKGLLS"
     gene            525..710
                     /locus_tag="VF402_11655"
     CDS             525..710
                     /locus_tag="VF402_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801001.1"
                     /translation="MTNASPEDKRAYLKAQRQRNLIIGLALAAFVVIVFFVSMSRMEQ
                     GLKRGAENAAASASAES"
     gene            715..1251
                     /locus_tag="VF402_11660"
     CDS             715..1251
                     /locus_tag="VF402_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7801002.1"
                     /translation="MAPQKRSHAKVVLSLIGAFLFMCAAVAVAVPLYRLFCPLSIQTG
                     ARNRAKEAPKVVTNRMMRVHFDSNTAAIPWTFKPDQPYQDVRVGKTAMAYFTVTNNAK
                     TAVTGRATYNILPDTMHAYFLKLQCFCFTDQTLQPGETKQFPVVYFLDPKLLKDPDTR
                     TVPDVTLSYTFFETDKGK"
     gene            1415..2290
                     /locus_tag="VF402_11665"
     CDS             1415..2290
                     /locus_tag="VF402_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7801003.1"
                     /translation="MAHHGVKHDYHIINPSPWPLMGSASAICWALGLVMGLRGLFGEP
                     KGSWWLFAVGIAGILLTMVFWWRDVIKESHKGDHTPVVQLGLRYGMIMFIASEVMFFV
                     AWFWIFFEMALFHGHRSGAAEAIDDVKTAWSTWPPKGVETLDPWQLPLVNTLLLLTSG
                     TTVTWAHHALQQGDRNGAKWGLLLTILLGMAFTAVQATEYSEIIREHLFFSEEASSAG
                     LYGSSFFMATGFHGFHVIIGTIFLIVCLIRLLAGDLTPKKHFGFEAAAWYWHFVDVVW
                     LFLFAFIYVIFGGAH"
     gene            2290..2646
                     /locus_tag="VF402_11670"
     CDS             2290..2646
                     /locus_tag="VF402_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX7801004.1"
                     /translation="MPEPAAGVNFAAAALGKCPVCGRGRLFRSWLKVADACDVCGTPF
                     KAAEAGDGPVVFVILIAGFLACAGLLTTFFMFPDWPVSAHLMIWPAFAVAVSLALMQP
                     LKGLMLASQLRHRVKD"
     gene            2648..3367
                     /locus_tag="VF402_11675"
     CDS             2648..3367
                     /locus_tag="VF402_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX7801005.1"
                     /translation="MKLSLSELARAAPRGMAVTVVICFVFLNGLGVWQLYRLHWKEGL
                     IAAMAHSEALPPVPVMDLLRTAKPDWRSARLPDCAVRGDRLIHMHSEMNGTPGYRVLT
                     ICPLPDGAMLTDLGFTTDKAAAPTTVLTPVGRLRPFEKSTAFTPVNRPGDGDWYWRSA
                     AEMGKALGAPVRGDYFLVLDLAASKAHMPGLVQGPLTAPLRNRHFEYALTWFGLAWAL
                     IGVFASFVYQRARQNKAAAAA"
     gene            3364..>3868
                     /locus_tag="VF402_11680"
     CDS             3364..>3868
                     /locus_tag="VF402_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7801006.1"
                     /translation="MTASLLEGQTLFQFDNGLRLLVDPMPGLRTFALNALVHGGARFE
                     TAAQSGWAHLLEHMVFKGAGGRDARALAEAIERQGGSINASTGYEHTRFEVRGLTQLL
                     PLALEVVGDLMFRPVLDGGELEREKKVIAQEILEAYDAPDDYVFDLLQGAMFPDQALG
                     RPILGDKA"
BASE COUNT          629 a         1218 c         1263 g          758 t
ORIGIN      
        1 acgccgcaga acatcgtcat cggcggcgcg gcgggcgcct ttccgccggt gatcggctgg
       61 gcggcggcga ccggccatgc gccgctggag gcgtgggtgc tgttcgccat catcttcctg
      121 tggacgccgc cgcacagctg ggcgctggcg ctctacacga ccgaggacta cgccaatgcg
      181 ggcataccga tgatgccggt ggtccggggg gccaaggcga cgcggctgca gatactgctc
      241 tattcgctgg tcatggcgcc ggtggcctgc ctgcctatcg cgttcggcac cggcggcgtc
      301 gcctatgcgg tcgtttcggt gctgggcggg gcggttttca tctatctggc ggtccgcgtc
      361 tttttgtcgc gggcgggaga tcatccgcaa ggtggcgagg ccggacttta tgacgttaaa
      421 aaagaggctc tggccgcgcg caacctgttc gcgttttcga ttctgtatct gaccttgctg
      481 ttcgccaccc tgctggccga acacgcgatt aaggggcttt tgtcatgacc aacgcgtcgc
      541 ccgaggacaa gcgggcttat ctgaaagcgc aaaggcaacg caatctgatc atcggtctgg
      601 cgctggccgc tttcgtcgtc atcgtcttct tcgtgtcgat gtcgcgcatg gagcaggggt
      661 taaagcgcgg cgccgaaaac gccgcggctt cggcctcggc ggagtcctaa gaccgtggcg
      721 ccgcagaaac gcagccatgc caaggtggtc ctgagcctga tcggggcgtt tctgttcatg
      781 tgcgcggcgg tggcggtcgc ggtgccgctg taccgcctgt tctgcccgct ctcgatccag
      841 acgggcgcgc gcaatcgcgc caaggaggcg cccaaggtcg tcaccaacag gatgatgcgg
      901 gtgcatttcg attccaacac cgcggcgatt ccctggacct tcaagccgga ccagccctat
      961 caggacgtgc gcgtcggcaa gacggcgatg gcttacttta cagtcaccaa caacgccaaa
     1021 accgcggtca ccggccgggc gacctacaac atcctgcccg acaccatgca cgcctatttc
     1081 ctgaagctgc aatgcttctg cttcacggat cagacgctgc aaccgggcga aaccaagcag
     1141 tttccggtgg tttacttcct cgatccgaag ctgctgaaag accccgacac caggaccgtg
     1201 ccggacgtca cgctgtccta taccttcttt gaaaccgaca aggggaaata gcgcgcaaaa
     1261 ataccgcgcg ccgaacggcg ttcgcattgt gttgcgtgga aaaacttggc ataaatccgt
     1321 tattcggcgc taaagccccg cacggattct gcgcccaacg accgataatc agccgcgatc
     1381 caaaaaagcg gcgctaacag tgagttgact caggatggcc catcacggcg ttaaacacga
     1441 ttatcacatc atcaacccca gtccctggcc cctgatggga tcggcttcgg cgatctgttg
     1501 ggcgctgggc ctcgtcatgg ggctgcgcgg cctgttcggc gagcccaagg gcagctggtg
     1561 gctgttcgcc gtcgggatcg ccggcatcct gctgacgatg gtgttctggt ggcgcgacgt
     1621 catcaaggaa agccataagg gcgatcatac gccggtcgtg caactgggcc tgcgctacgg
     1681 catgatcatg ttcatcgcct ccgaagtgat gttcttcgtg gcgtggttct ggatcttctt
     1741 cgaaatggcc ctgttccacg gccaccgctc cggcgccgcc gaggcgatcg acgacgtgaa
     1801 gaccgcgtgg tcgacctggc cgcccaaggg cgtcgagacg ctcgatccgt ggcagttgcc
     1861 gctggtcaac accctgctgc tgctgacctc cggcacgacg gtgacctggg cgcaccacgc
     1921 gctgcaacag ggcgaccgca acggcgccaa gtgggggctt ttgctgacca tcctgctggg
     1981 catggccttc acggcggtgc aggcgaccga atacagcgag atcatccgcg agcacctgtt
     2041 cttctccgaa gaagccagca gcgcgggcct ctatggctcc agcttcttca tggcgacggg
     2101 cttccacggc ttccacgtca tcatcggcac catcttcctg atcgtctgcc tgattcggct
     2161 gctggccggg gatctcacgc cgaaaaagca tttcggcttc gaggcggcgg cctggtactg
     2221 gcacttcgtc gacgtggtgt ggctgttcct gttcgccttc atctacgtga tcttcggcgg
     2281 ggcgcattga tgccggaacc ggcggcgggc gtgaacttcg cggcggccgc gctcggcaaa
     2341 tgcccggtct gcggccgggg caggctgttc aggtcatggc tcaaggtcgc ggacgcgtgc
     2401 gatgtttgcg ggacgccttt caaggcggcg gaggccggcg acggccccgt cgtcttcgtc
     2461 attctcatcg ccggatttct ggcctgcgcg gggttgctga cgaccttttt catgttcccg
     2521 gactggccgg tttcagcgca cctgatgatc tggcccgcct ttgcggttgc ggtcagcctg
     2581 gcgctgatgc agccgttgaa gggcctgatg ctggcgtcgc aattgcggca tcgcgtgaag
     2641 gactgacatg aagctcagcc tttccgaact ggcgcgcgcc gcgccgcgcg gcatggcggt
     2701 caccgtcgtc atctgcttcg tgtttctgaa cggtctcggc gtctggcaat tgtatcgcct
     2761 gcactggaaa gaggggctga tcgccgccat ggcgcacagc gaagccctgc cgcccgtccc
     2821 ggtcatggac ctgctcagga cggcgaaacc ggactggcgc tcggcgcgtt tgccggactg
     2881 cgcggtccgc ggcgatcgcc tgatccacat gcattccgaa atgaacggca cgcccggtta
     2941 ccgcgtgctg acgatctgcc cgctgcccga cggcgccatg ctgaccgatc tcggttttac
     3001 gaccgacaag gccgctgcgc cgacgacggt tctaacgccg gtggggcggt tgcggccgtt
     3061 tgaaaaaagc accgccttca cgccggtcaa ccggcccggc gacggcgact ggtattggcg
     3121 ttcggcggcg gagatgggca aggcgctggg cgcgccggtg cgcggcgatt atttcctggt
     3181 gcttgatctg gccgcgtcaa aggcccatat gccgggactt gtgcaggggc cgctgaccgc
     3241 gccgctgcgc aatcgacatt ttgaatacgc cctgacctgg ttcgggctgg cctgggcgct
     3301 gatcggcgtt ttcgccagct tcgtctatca gcgggcgcga cagaacaagg ccgccgccgc
     3361 cgcatgaccg cttccctgct ggaaggacaa acccttttcc aattcgacaa cggcctgcgc
     3421 ctgctggtcg acccgatgcc cggtttgcgc accttcgcgc tcaatgcgct ggtccacggc
     3481 ggcgcgcgct tcgagacggc ggcgcagtcg ggctgggcgc acctgctgga gcatatggtg
     3541 ttcaagggcg cgggcggacg cgacgcgcgc gccctggccg aggccatcga gcggcagggc
     3601 ggcagcatca acgcctcgac cggttatgag catacccgtt tcgaggtgcg cggcttaacg
     3661 caattgctgc cgctggcgct ggaggtggtc ggcgacctga tgttccggcc cgtgctggac
     3721 ggtggcgagc tggagcgcga aaagaaggtg atcgcgcagg aaatcctcga agcctatgac
     3781 gcgccggacg actatgtttt cgacctgttg cagggcgcga tgtttcccga tcaggcgctg
     3841 gggcgtccga ttctgggcga caaggcca
//
LOCUS       DASVLL010000711         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_320737,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000711 DASVLL010000000
VERSION     DASVLL010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320737"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VF402_11685"
     CDS             complement(<1..1520)
                     /locus_tag="VF402_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX7801007.1"
                     /translation="MLRAALAASVLALMAAAPAPAQTPVTPMTPDMVAAYDKTRPQAD
                     FVRQAVMIPMRDGVKLYTVIVYKKGTTHAPILLSRTPYDARSSTARLDSQSIDDILPV
                     MDKDFVDDGYIRVYQDIRGIHNSEGDYVMNRPIAGPLNPTGIDESTDAYDTIDWLVKH
                     LKNSNGRVGVIGSSYLGFTTLMAELNPHPALKAAAPQSPMVDGWMGDDWFHNGAFRNV
                     NLSYVVEQTTGKAESGATLAHGPGDDYTRFLEAGSTGDYVRAWGAQDYPFMRKLMAHP
                     AYDGFWSGQAVDKWMAAAPLTVPTMLIVGQWDQEDSYGAPAVYRAMEPKDAGNDKVSL
                     VIGPWRHSGVNHYGYALGDLTFDGDTAQQFRAIYLKPFFDHYLKDAPDPKTPPVLTYA
                     TGVNRWEVSQKWPVGTPTPLYLGADGAAGFDAPSAAGHDDYVSDPARPVPFLPRPIDM
                     DDTKQWTTWLVHDQRFVDDRPDVLTYQTPVLDAPVHIMGAPRVDLFAATTGTDSDWV"
BASE COUNT          282 a          551 c          614 g          310 t
ORIGIN      
        1 acccagtcgg agtccgttcc ggtggtggcg gcgaacaggt cgacccttgg cgcgcccatg
       61 atatgcaccg gcgcgtcgag aaccggcgtc tgataggtca gcacgtcggg acgatcgtcg
      121 acgaagcgct ggtcgtgcac gagccaggtc gtccactgct tcgtgtcgtc catgtcgatc
      181 gggcgcggca ggaagggcac gggcctggcc gggtcggaga cgtaatcgtc atgtcccgcc
      241 gcggacggcg cgtcgaaacc ggcggcgccg tccgcgccga gatagagcgg cgtcggcgtt
      301 cccaccggcc atttttgcga aacctcccag cggttgacgc ccgtggcgta ggtcagcacg
      361 ggcggcgtct tcgggtccgg cgcgtctttc agatagtggt cgaagaacgg cttgaggtag
      421 attgcgcgga actgttgcgc cgtgtcgccg tcgaaggtca ggtcgcccag cgcgtagccg
      481 taatggttga cgccggaatg ccgccatggc ccgatgacca gcgacacctt gtcgttgccg
      541 gcgtccttcg gctccatggc gcgatagacg gcgggggcgc cgtaggaatc ctcctgatcc
      601 cactggccca cgatcagcat ggtcggcacc gtcagcggcg cggcggccat ccacttgtcg
      661 accgcctgac cggaccagaa gccgtcatag gccggatgcg ccatcagctt gcgcatgaac
      721 ggatagtcct gcgcgcccca tgcgcgcaca tagtcgccgg tcgaaccggc ttcgagaaag
      781 cgcgtgtaat cgtcgcccgg cccgtgcgcc agcgtggcgc cgctttcggc cttgccggtg
      841 gtctgctcga ccacatagga caggttgacg ttgcggaagg cgccgttgtg gaaccagtcg
      901 tcgcccatcc agccgtcgac catcggcgat tgcggcgcgg cggccttcag cgccggatgc
      961 gggttcagct ccgccatcag ggtcgtgaag cccagatagc tggagccgat gacgccgacc
     1021 ctgccgttgc tgttcttgag gtgcttcacc agccagtcga tggtgtcgta ggcgtcggtc
     1081 gattcatcga tgccggtggg gttgagcggc ccggcgatag gccggttcat cacgtaatcg
     1141 ccctcggaat tgtggatgcc gcgaatgtcc tgatagacgc ggatatagcc gtcgtcgacg
     1201 aagtccttgt ccataacggg caggatgtcg tcgatcgact ggctatccag ccgcgcggtc
     1261 gagcttcggg cgtcgtaggg cgtgcgcgac aacaggatcg gcgcgtgcgt cgtccccttc
     1321 ttatagacga tcaccgtgta cagcttcacg ccgtcgcgca tcgggatcat cacggcctgc
     1381 cggacgaaat cggcctgggg ccgcgtcttg tcgtaggccg cgaccatgtc cggcgtcatg
     1441 ggagtcaccg gcgtctgggc cggagccggg gctgcggcca ttagggcgag gacggaggcg
     1501 gcgagggcgg cgcggagcat ggcggattcc tgaaggcttg gcctgaacgg cgggcgacag
     1561 cgcgatccta cacgcctgcc cgcgcccgac aacggcaaaa gtttcagatg cgcctgaacg
     1621 tgcgccggcg cgcggcttga ctccgtgcgt gagtcgcgcc cccatgccgt ccttcgcgag
     1681 gagacgcaca tgggcgacat caaactggag gacggctgga aggcgctgct ggaggcggag
     1741 ttctccaagc cgtatat
//
LOCUS       DASVLL010000712         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_535925,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000712 DASVLL010000000
VERSION     DASVLL010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535925"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1238
                     /locus_tag="VF402_11690"
     CDS             276..1238
                     /locus_tag="VF402_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7801008.1"
                     /translation="MTSTPQTRPNWPDFRHFRPAPATASALSVAVAALLVLIKLYATV
                     VSGSSSVLASMTDSGLDLVASLVTFFAVRYAKTPPDKEHPFGHGKAEAFSALFQAGLV
                     FASAALVMQACVRHLMHPEPLQQTGLAVAVLIVSIVLTLGLVSFQNMALRGAQSVAVS
                     ADRMHYLTDLVTNLIALAGVAAAILGFPAMDAAAGFIMAGWFVWGAVQVLREAASHLM
                     DEALGDADVARLKALALEDPAITGVHELRTRLSGPYIAVQMHVEMDPRLSLAEAHRII
                     IAAETRLLAAYPNADILIHPDPRGEAEPHGGVFHQEDGSAPEAL"
     gene            1271..1984
                     /locus_tag="VF402_11695"
     CDS             1271..1984
                     /locus_tag="VF402_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7801009.1"
                     /translation="MSPTPVPYVRQLYHFAFDPHSRLARLALGEKKLSFEEVPVRYWE
                     PDDAFLRLNPSGLLPVLVETPEAGAQGQTHRICENRALLEHLEEAAPAPNLWPQDVNA
                     RAEARRLVGWFERKFDYEVNALLLHEKMEKRLIGAGAPDIGAMRAGREALKDHLRYFE
                     SLLATRDWLAGQSLSYADFACAAQLSVLDYFDEINWTRYPHLKTWYMVMKSRPCFRPL
                     LADKLPGVPASRHYAELDF"
     gene            1981..>2801
                     /gene="queG"
                     /locus_tag="VF402_11700"
     CDS             1981..>2801
                     /gene="queG"
                     /locus_tag="VF402_11700"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX7801010.1"
                     /translation="MTPGADLADTIRAEAQALGFCAARFAPLPDVWAASGRLEAFVAA
                     GLHGDMRWMEETLARRRHPRNMWDGARSALVLGYNYGPDSDPRDKLAAPDLANISCYA
                     RNEDYHELIKKKLKQLATWIVARTGCELKVFVDTAPLMEKPLAQLAGLGWQGRHTNLV
                     SRDFGSWLFLGVILFDRELGEDAPERDHCGSCRACLDACPTDAFIAPYRLDARKCLSY
                     LTIEYRGAWPEAFRRAAGNRIYGCDDCLAACPWNKFAQEAQDIRLQTRDGFDALKL"
BASE COUNT          436 a          931 c          944 g          490 t
ORIGIN      
        1 gtaagcatag agggtttgcg gcttgacctc gagacgcttc agcgcctcgg aacggctgat
       61 ccagactcgt gacatgcgac gcgccctaaa cctgtggttc tgcgaacgga attcttatcc
      121 ggaaatcaag atcgtcagaa aatcaagatt gattccggac aatgccacgt ctcctccgcg
      181 aaggctacgc cgcatttagg tttcattaag atatttcgcg cgcgacatcg atcccgcgcg
      241 cttgcgtcac cttggcggca tgataagaca tttccatgac ctccacgcca cagacgcgcc
      301 ccaattggcc tgatttcagg catttccgtc ccgcgcccgc caccgcgtcc gccctgtcgg
      361 tcgcggtggc ggcgctgctg gtgctgatca agctctatgc gacggtcgtg tcgggttcgt
      421 cctcggtgct ggcctcgatg accgattccg gcctcgacct ggtcgcctcg ctggtgacct
      481 tcttcgccgt gcgctacgcc aagacgccgc cggacaagga gcatccgttc ggccacggca
      541 aggccgaggc gttctcggcc ctgtttcagg ccgggctggt cttcgcctcc gccgccctgg
      601 tcatgcaggc gtgcgtgcgc catctcatgc accctgagcc gttgcagcag accgggctgg
      661 ccgtcgccgt gctgatcgtg tcgatcgtgc tgaccctggg gctggtcagc tttcagaaca
      721 tggcgctgcg cggggcgcag tcggtggccg tgtccgccga ccggatgcat tacctgaccg
      781 acctcgtcac caacctgatc gcgctggcgg gcgtggcggc ggcgatactg ggctttccgg
      841 cgatggacgc cgccgcgggc ttcatcatgg cgggctggtt cgtctggggc gcggtgcagg
      901 tgctgcgcga ggcggcgtcg cacctgatgg acgaggcgct gggcgatgcg gacgtggcgc
      961 ggctcaaggc gctggcgctg gaagacccgg cgatcacggg cgtgcatgag ctgcgcacgc
     1021 gcctgtccgg gccgtacatc gccgtgcaga tgcatgtcga gatggacccg cgcctgagcc
     1081 tggccgaggc gcaccgcatc atcatcgccg ccgagacccg gctgctggcc gcctatccga
     1141 acgccgacat cctgatccat cccgatccgc gcggcgaggc cgagccgcac ggcggcgttt
     1201 tccaccagga agacgggagc gcgcccgaag cgctttgact cccctgaggc gaataaggca
     1261 taagcgatac atgtccccca cgcccgtccc ctatgtgcgc cagctttatc atttcgcctt
     1321 cgatccgcat tcccggctgg cgcggctggc gctgggcgaa aagaagctga gcttcgagga
     1381 ggttccggtg cgttactggg agccggacga cgcgttcctg cggctcaacc cgtccggcct
     1441 gctgccggtg ctggtcgaga cgccggaggc gggcgcgcag ggccagacgc acaggatctg
     1501 cgagaaccgc gccctgctcg aacatctgga ggaggccgcg cccgcgccga acctgtggcc
     1561 gcaggacgtc aacgcgcgcg ccgaggcgcg ccgcctcgtc ggctggttcg agcgcaagtt
     1621 cgactatgag gtcaacgcgc tgctgctgca cgaaaagatg gagaagcggc tgatcggggc
     1681 gggggcgccc gacatcggcg ccatgcgcgc gggccgtgag gcgctgaagg accacctgcg
     1741 ctatttcgag agccttctgg cgacgcgcga ctggctggcg ggccagagcc tgtcctacgc
     1801 cgacttcgcc tgcgcggcgc agttgtcggt gctggactat ttcgacgaga tcaactggac
     1861 gcgctatccg cacctgaaga cctggtacat ggtgatgaag tcgcgcccgt gcttccgccc
     1921 gctgctggcg gacaagctgc ccggcgtgcc ggcgtcgcgg cattacgccg agctggattt
     1981 ttgacgcccg gcgcggacct cgccgacacg atccgcgccg aggcgcaggc gctcggtttc
     2041 tgcgccgcgc gtttcgcgcc cctgcccgac gtgtgggcgg cgtccgggcg gctggaggcg
     2101 ttcgtggcgg cggggctgca cggcgacatg cgctggatgg aggagacgct ggcgcggcgc
     2161 aggcacccgc gcaacatgtg ggacggcgcg cgctcggcgc tggtgctggg ctataattac
     2221 ggccccgaca gcgatccgcg cgataagctg gccgcgccgg acctcgccaa catcagttgc
     2281 tatgcgcgca acgaagatta tcacgagctg atcaagaaga agctgaagca actcgcgacc
     2341 tggatcgtgg cgcggaccgg ctgcgaactc aaggtgttcg tcgacaccgc gcccttgatg
     2401 gaaaagccgc tggcgcaact ggccgggctg ggctggcagg gtcggcacac caatctcgtg
     2461 tcgcgcgatt tcggctcgtg gctgtttctc ggcgtcatcc tgttcgaccg cgaactgggc
     2521 gaggacgcgc cggagcgcga tcattgcggc tcgtgccgcg cctgcctcga cgcctgcccg
     2581 accgacgcct tcatcgcgcc ctaccggctg gacgcgcgca aatgcctgtc ctatctgacc
     2641 atcgaatatc gcggcgcctg gccggaagcc ttccggcgcg cggccggcaa ccgcatctat
     2701 ggctgcgacg actgcctggc cgcctgtccg tggaacaagt ttgcgcaaga agcgcaggat
     2761 atccgcctgc aaacgcgcga cggtttcgac gcgctgaaac t
//
LOCUS       DASVLL010000713         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_373037,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000713 DASVLL010000000
VERSION     DASVLL010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373037"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF402_11705"
     CDS             complement(<1..308)
                     /locus_tag="VF402_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEX7801011.1"
                     /translation="MLDQGMKAQLKAYLENLRVPIALTAHVDDGAKSRELIDLLEDIV
                     AASDKVSLSVKNGSDAARVPSFDITRPGSDIAVTFAGLPMGHEFTSLVLALLQVGGHP
                     P"
     gene            complement(513..1076)
                     /gene="ahpC"
                     /locus_tag="VF402_11710"
     CDS             complement(513..1076)
                     /gene="ahpC"
                     /locus_tag="VF402_11710"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEX7801012.1"
                     /translation="MALINTAIKPFKATAYKQGKFVDISDADTKGKWSIFFFYPADFT
                     FVCPTELEDLAGIYPELRKLNVEVFSVSTDTHFSHKAWHDTSPAIGKIDYYMVGDQSG
                     QITNNFDVMRPGVGLADRGTFLVDPDGIIQFMEITSEGVGRNATELLRKVKAAQYVAS
                     HPGEVCPAKWEEGEKTLAPSLDLVGKI"
     gene            complement(1169..1723)
                     /locus_tag="VF402_11715"
     CDS             complement(1169..1723)
                     /locus_tag="VF402_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7801013.1"
                     /translation="MSLDSLYALIPAYARDLAQNLSVLADETVLDDQQKWGVFLACAH
                     AVGVPPVVRAFEAHAGALLTPEAVTAARAAAAVMGMNNVYYRALHLMRNKAYEAMPSK
                     LRMNALRDPGVSKVDFELWCLAVSAVNGCGLCLDSHEKALREHGAPALQVQAALRIAS
                     VVNALSAVMRAEAALGSIPAFENK"
     gene            1844..>2620
                     /locus_tag="VF402_11720"
     CDS             1844..>2620
                     /locus_tag="VF402_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7801014.1"
                     /translation="MLPTLRQLQYLKLLAEHKSFMAAAEAAFVSQPALSSGIAELEKT
                     LGARLVDRSRGQVILTPVGEETLKRAEEILARTEDLVEAARGANRPLSGRFRLGVIPT
                     IAPFLLPGALARLRAAYPDLRLFLREDQTARLIGALKSGALDAAVIALPWDLNGLDHA
                     FVMRDAILAATPNGHPLAKETRIAPEALKHEEMILLEDGHCLREHALAACGLRPPKAA
                     PGEEVFAATSLPTLVQMVGSGLGVTFLPAMAVEAGLTDAAP"
BASE COUNT          444 a          842 c          861 g          473 t
ORIGIN      
        1 ggcgggtggc cgccgacctg caacagggcc agcaccagcg aggtgaattc atgccccatc
       61 ggcaggcccg cgaaggtcac ggcgatgtcg gagccggggc gcgtgatgtc gaaggacggg
      121 acgcgcgccg cgtccgagcc gttcttcacg ctcaggctca ccttgtccga cgccgccacg
      181 atgtcttcca agaggtcgat cagttcgcgc gacttcgcgc cgtcatcgac atgcgccgtc
      241 agtgcgatgg gaacgcgcag gttctccaga taggctttca gttgcgcctt catgccttgg
      301 tcgagcatgg cttgctcctc gaatttcagg gggattcatt gtccatattt acggttccgg
      361 gagaaccgca gataaggaga atgaagttcc ccctcccacc gcttcgcggt cccccctccc
      421 cgctgcgcag ggagggatgg gggcgcctca tcctcccccg tttacggggg aggcggcgtt
      481 tgcgaagcaa acgacggagg gggcgagcag acctagatct tgccgaccag atccagcgac
      541 ggggccaggg tcttttcgcc ttcttcccac ttggccgggc agacttcgcc ggggtgcgag
      601 gcgacgtatt gcgcggcctt gaccttacgc agcagttccg tcgcgttgcg gccgacgcct
      661 tcggaggtga tctccatgaa ctggatgatc ccgtccgggt cgaccaggaa ggtgcctcgg
      721 tcggccaggc cgacgcccgg acgcatcacg tcgaaattgt tggtgatctg gcccgactgg
      781 tcgccgacca tgtaatagtc gatcttgccg atggcgggcg aggtgtcgtg ccacgccttg
      841 tgcgagaaat gcgtgtcggt cgaaacggag aagacttcga cgttcagctt ccgcagttcg
      901 ggatagatgc cggccaggtc ttccagctcg gtcgggcaca cgaaggtgaa gtcggccgga
      961 tagaagaaga agatcgacca cttgcccttg gtgtcggcgt cggaaatgtc gacgaacttg
     1021 ccttgcttgt aggcggtcgc cttgaacggc ttgatggcgg tgttgatgag ggccatgagg
     1081 aggtctcctt gcttgggggt tgatgacagt caaaaaccat attcgccgca atagggcaaa
     1141 tctattttat ttttatttcg gataaatttt atttattctc gaaagccgga atcgatccca
     1201 gcgccgcctc ggcccgcatc accgccgaca gcgcgttgac gaccgaggcg atgcgcaggg
     1261 ccgcctgcac ctgaagcgcg ggcgcgccgt gttcgcgcag cgccttttca tggctgtcca
     1321 ggcacaggcc acagccgttc accgccgaca ccgccaggca ccacagctcg aaatcgacct
     1381 tgctgacgcc cggatcgcgc agcgcgttca tgcgcagctt cgacggcatc gcctcatacg
     1441 ccttgttacg catcaggtgc agcgcccggt aatagacatt gttcatgccc atgaccgccg
     1501 ccgccgcgcg cgccgccgtg acggcttcgg gcgtcagcag cgccccggcg tgcgcctcga
     1561 acgcgcgcac caccggcgga acgccgaccg cgtgggcgca ggccagaaag actccccatt
     1621 tctgctggtc gtccagaacc gtctcgtcgg ccagcacgct gagattttgc gccaggtcgc
     1681 gcgcataggc cggaatgagg gcgtaaaggg aatcgagcga catcgtcatc cttgggttcg
     1741 cggaaaatat ggagcggcga atctgcgccg gaacgcgcgc ggatgaaaca aaaattccat
     1801 ttcggttttt ggcgtcggca taaatcgtgc ttatatccgc ttcatgctgc cgaccctgcg
     1861 ccaattgcaa tatctgaaac tgctcgccga gcataagagc ttcatggcgg cggcggaagc
     1921 ggccttcgtg tcgcagcccg ccctgtcgtc cggcatcgcc gaactggaaa agacgctggg
     1981 cgcgcgcctc gtcgaccgct cgcgcgggca ggtgatcctg acgccggtgg gcgaggagac
     2041 gctgaaacgc gccgaggaga ttctggcccg caccgaggat ctggtcgagg cggcgcgcgg
     2101 cgccaaccgg cccctgtccg ggcgtttccg gctgggcgtg atcccgacca tcgcgccgtt
     2161 cctgctgccc ggcgcgctgg cgcggctgcg cgcggcgtat cccgacctgc ggcttttcct
     2221 gcgcgaggac cagacggcgc ggctgatcgg cgcgctcaaa agcggcgcgc tggacgccgc
     2281 cgtcatcgcc ctgccgtggg atctgaacgg gctggaccac gccttcgtca tgcgcgacgc
     2341 gattctggcg gcgacaccga acgggcatcc gctggcgaag gaaacgcgca tcgcgccgga
     2401 ggcgttgaag cacgaggaga tgatcctgct ggaggacggc cattgcctgc gcgagcacgc
     2461 gctcgccgcc tgcggcctgc gcccgcccaa ggcggcgccg ggcgaggagg tcttcgccgc
     2521 cacctcgctg ccgaccctgg tgcagatggt gggatcgggc ctcggcgtga ccttcctgcc
     2581 ggccatggcc gtggaggccg gcctcaccga cgccgccccc
//
LOCUS       DASVLL010000714         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_265717,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000714 DASVLL010000000
VERSION     DASVLL010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265717"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /gene="dnaK"
                     /locus_tag="VF402_11725"
     CDS             <1..1662
                     /gene="dnaK"
                     /locus_tag="VF402_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7801015.1"
                     /translation="DPEVAKDQKLVPFKIVKGTNGDAWVESHGKQYSPQQISAFILQK
                     MKETAEAKLGEKVTQAVITVPAYFNDAQRQATKDAGKIAGLEVLRIINEPTAAALAYG
                     LDKKKDAHTIAVYDLGGGTFDVSILEIGDGVFEVKSTNGDTFLGGEDFDMRVVDYLAD
                     EFKKENGVDLRSDKLALQRLKEEAEKAKKELSTVAQYDLNLPFISMNASGPLHLNIKL
                     TRAKLESLVEDLIQKTIAPCQQALKDAGLKASDIDEVVLVGGMTRMPKVQDAVKTFFG
                     REPHKGVNPDEVVALGAAIQAGVLQGDVKDVLLLDVTPLTLGIETLGGVFTPLIERNT
                     TIPTKKSQTFSTADDNQSAVTIRVFQGERPMAADNKLLGQFDLVGIPPAPRGVPQIEV
                     TFDIDANGIVNVHAKDKATNKEHSIRIQANGGLSDADIEQMVKDAEANKAEDEKRKVL
                     IEARNQADALIHSTEKSLKEFGDKVAADDKTAIETALNDLKTAKDGEDAEDIKAKSQT
                     LIQASMKLGEAMYGQQGGEEAAEAAGDDNVVDAEFEEVDDDKKGA"
     gene            1865..>2656
                     /gene="dnaJ"
                     /locus_tag="VF402_11730"
     CDS             1865..>2656
                     /gene="dnaJ"
                     /locus_tag="VF402_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7801016.1"
                     /translation="MSKRCYYEVLGVERTVDEVTLKTAFRKKAMEHHPDRNHGDAGAE
                     TRFKEVNEAYSILSDGDKRAAYDRFGHDGVNGGGAGGFGGFGGGAGFSNVEDIFSEMF
                     GDIFGQQQRRPGGAQRGADVRFDYEITLEQAYRGAEVEITVPTTQTCESCHGSGAKAG
                     TRPQTCSTCGGHGRVRASNGFFAVERACPACNGQGQTIKEPCPDCRGHGQVRKNRDLK
                     VRIPAGVDDGARILLKGEGQAGAKGGPKGDLYIFLSVAEHDLFERD"
BASE COUNT          568 a          854 c          857 g          377 t
ORIGIN      
        1 gaccccgagg tcgcgaagga ccaaaagctc gtcccgttca agatcgtcaa aggcacgaac
       61 ggagacgctt gggtcgaaag ccacggcaag cagtattcgc cgcaacagat ctccgccttc
      121 atccttcaga agatgaagga aaccgccgaa gccaagctcg gcgagaaggt cacccaggct
      181 gtcatcacgg ttccggccta cttcaacgac gctcagcgcc aggccacgaa agacgccggg
      241 aagatcgcgg gtcttgaggt cctgcgcatc atcaacgagc cgacggcggc tgcacttgcc
      301 tacggcctcg acaagaagaa ggacgctcac accatcgcgg tctacgacct cggcggcggc
      361 acgttcgacg tgtcgatcct cgaaatcggc gacggcgtct tcgaagtgaa gtcgaccaac
      421 ggcgacacct tcctgggcgg cgaagacttc gacatgcgcg tcgtcgacta tctggccgac
      481 gagttcaaga aggagaacgg cgtcgacctg cgctccgaca agctggccct gcaacgcctc
      541 aaggaagagg cggaaaaggc caagaaggag ctgtcgaccg tcgcgcagta cgacctgaac
      601 ctgccgttca tctcgatgaa cgcgtcgggt ccgctgcacc tgaacatcaa gctgacccgc
      661 gccaagctgg aatcgctggt cgaggatctg atccagaaaa ccatcgcgcc gtgtcagcaa
      721 gcgttgaaag acgcgggcct caaggcgtcg gacatcgacg aagtggtgct ggtcggcggc
      781 atgacccgca tgcccaaggt gcaggacgcg gtcaagacct tcttcggccg cgaaccgcac
      841 aagggcgtca acccggacga agtcgtggcg ctgggcgccg ccattcaggc gggcgtgctg
      901 caaggcgacg tcaaggacgt gctgctgctc gacgtcactc cgctgaccct gggcattgag
      961 acgctgggcg gcgtgttcac cccgctgatc gagcgcaaca ccaccatccc gaccaagaag
     1021 tcgcagacct tctcgaccgc cgacgacaac cagtcggccg tgacgatccg cgtcttccag
     1081 ggcgaacgcc cgatggccgc cgacaacaag ctgctgggtc agttcgacct cgtgggcatc
     1141 ccgcccgcgc cgcgcggcgt gccgcagatc gaggtcacct tcgacatcga cgccaacggc
     1201 atcgtcaacg tccacgccaa ggacaaggcg acgaacaagg agcactcgat ccgcattcag
     1261 gccaatggcg gcctgtccga cgccgacatc gagcagatgg tcaaggacgc cgaggccaac
     1321 aaggccgagg acgaaaagcg taaagtgctg atcgaagccc gcaatcaggc cgacgccctg
     1381 atccactcga ccgaaaagtc gctgaaggaa ttcggcgaca aggtcgcggc ggacgacaag
     1441 acggcgatcg aaaccgccct taacgacctg aaaacggcca aggacggcga ggacgccgag
     1501 gatatcaagg ctaagtccca gacgctgatt caggcgtcga tgaagctggg cgaagccatg
     1561 tacggccagc aaggcggcga ggaagccgcc gaagccgccg gtgacgacaa cgtcgttgac
     1621 gccgaattcg aggaagtcga cgacgacaag aagggcgcgt aagcgttccg gccatgacgc
     1681 cgcttcgatc cttttcggcg aatgcggagg ccgctaaaat cgtgcggacg gcggcggggt
     1741 ttgactcctc gccgccgtca cggcacaaac tctttcccag atatgacaat ctgaccgccc
     1801 gattcggacg ccgtgactgt gcgcgccgcg ggcggacgcc aatccggcga ttcggggcag
     1861 taagatgagc aagcgttgtt attatgaagt gctgggggtc gagcgcaccg tcgatgaggt
     1921 gacgctgaaa accgccttcc gtaaaaaggc gatggagcat caccccgacc gcaatcacgg
     1981 cgacgccggg gccgagacgc gcttcaagga ggtcaacgag gcctattcga tcctgtccga
     2041 cggcgacaaa cgcgcggcct atgaccgctt cggccatgac ggcgtgaacg gcggcggcgc
     2101 gggcggtttc ggcggcttcg gcggcggcgc cggtttctcc aacgtcgagg acatcttctc
     2161 ggaaatgttc ggcgacatct tcggccagca gcagcgacgt ccgggcgggg cgcaacgcgg
     2221 ggccgacgtg cgcttcgatt acgaaatcac gctggagcag gcctatcggg gcgccgaggt
     2281 cgagatcacc gtcccgacca cgcagacctg cgaatcctgc cacggctccg gcgccaaggc
     2341 gggcacgcgc ccgcagacct gttcgacctg cggcggtcat ggccgcgtgc gcgcctccaa
     2401 cggcttcttc gccgtcgagc gcgcctgccc ggcctgcaac ggtcagggcc agaccatcaa
     2461 ggagccgtgc cccgattgcc gcggccacgg tcaggtgcgc aagaaccgcg acctgaaggt
     2521 gcgcatcccg gcgggcgtcg acgacggcgc gcgcatcctg ctgaaaggcg agggtcaggc
     2581 gggcgccaag ggcgggccga agggcgatct ctacatcttc ctgagcgtgg ccgagcacga
     2641 cctgttcgag cgcgac
//
LOCUS       DASVLL010000715         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_427258,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000715 DASVLL010000000
VERSION     DASVLL010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427258"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VF402_11735"
     CDS             <1..735
                     /locus_tag="VF402_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7801017.1"
                     /translation="ADFTRLHPDLNGVDPDDYFSDVPYEKGAAFLRMLEAHFGRKRLD
                     AYLKGYFHRYAFQSMTTDAFVVDLRENLLHNDADLEKSLKIHQWLYDKGLPANAVAPA
                     SSLLDQVRTQAAAFAAGTAPDALQTGDWSSLQWQYFLTQLPDSLTTDQMTTLDKAYAF
                     TATHNDEILFNWLMLAVKHHYQPAMPALHDFLVSQGRMKYVTPLYKALAAQPGWGTDM
                     AKKIYAEAEPGYHELTRADVAKVLPK"
     gene            complement(736..1629)
                     /locus_tag="VF402_11740"
     CDS             complement(736..1629)
                     /locus_tag="VF402_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7801018.1"
                     /translation="MKRDDLSDIQTLLTVVREGGFTRAAAKIGVSPSALSHALRALEE
                     RLGVRLLTRTTRSLAPTEAGERLIRNVGPHLDGIAAELAALGELRDKPAGTIRITSGE
                     HSAETILWPKLAPLLRAYPDIRVEITVDYGLTDIVAERFDAGVRLGEQVAQDMIAVRV
                     GPDMRMAVVAAPACFAARGRPRTPQDLTQHDCINLRLPTRGGVYAWEFEKDGREVRVR
                     VEGQSVFNSTPLILRAALDGAGLAYLPLDRVRPYLDDGRLEQALEDWLPPFAGYHLYY
                     PSRRQLSPAFALVVEALRYRP"
BASE COUNT          327 a          673 c          604 g          286 t
ORIGIN      
        1 gcggacttca cccggctgca tcccgacctc aacggcgtcg atcccgacga ctatttcagc
       61 gacgtgccgt atgaaaaggg cgccgccttc ctgcgcatgc tggaggcgca tttcgggcgc
      121 aagcggctgg acgcctatct caagggctat ttccaccgct acgccttcca gagcatgacc
      181 accgacgcct tcgtcgtgga cctgcgcgaa aacctgctgc acaacgacgc cgatctggag
      241 aagtcgctga aaatccatca gtggctgtac gacaagggcc tgcccgccaa cgccgtggcg
      301 ccggcctcca gcctgctgga tcaggtcagg acgcaggccg ccgccttcgc cgccggaacc
      361 gcgccggacg ccttgcagac cggggactgg tcgtcgctgc aatggcagta tttcctcacg
      421 caactgcccg acagcctgac gacggaccag atgacgacgc tggacaaggc ctacgccttc
      481 accgcgacgc ataatgacga gatcctgttc aactggctga tgctggcggt gaagcaccac
      541 tatcagcccg ccatgccggc cctgcacgac ttcctcgtct cgcaggggcg catgaaatac
      601 gtcacgccgc tgtataaggc gctggccgcc cagcccggct gggggacgga catggcgaag
      661 aagatctacg ccgaggccga gccgggctat cacgaactga cccgcgccga cgtcgccaag
      721 gtgctgccga aatagtcacg gccggtagcg cagcgcctcg acgaccagcg cgaaggcggg
      781 cgacaactgg cggcggctcg gatagtacag gtgatagccc gcaaacggcg gcagccagtc
      841 ctccagcgcc tgttcaagcc gcccgtcgtc gagatagggc cgcacccggt ccagcggcag
      901 ataggccaga cccgccccgt ccagcgcggc gcgcaggatc agcggcgtgg aattgaacac
      961 gctctggccc tcgacgcgca cgcgcacctc gcgcccgtcc ttctcgaatt cccaggcata
     1021 gacgccgccg cgcgtcggca ggcgcagatt gatgcaatcg tgctgcgtca gatcctgcgg
     1081 cgtgcgcggc cgtccgcgcg ccgcgaaaca ggcgggcgcc gcgaccacgg ccatgcgcat
     1141 gtccggcccg acgcgcaccg cgatcatgtc ctgcgccacc tgctcgccca gccgcacgcc
     1201 cgcgtcgaag cgctcggcca cgatgtcggt caggccgtaa tccaccgtga tctccacgcg
     1261 gatgtcggga taggccctga gcaggggcgc cagtttcggc cacaggatcg tctcggcgga
     1321 atgctcgccc gaggtgatgc ggattgtccc ggcgggcttg tcgcgcagtt cgcccaaggc
     1381 cgccagttcg gcggcgatgc cgtcgagatg cgggccgacg ttgcggatca gccgctcccc
     1441 ggcctcggtc ggcgccaggc tgcgcgtggt gcgcgtcagc agccgcaccc ccagccgttc
     1501 ctccagcgcg cgcagggcat ggctgagcgc cgagggcgac acgccgatct tcgccgccgc
     1561 gcgcgtgaag ccgccttcgc gcacgacggt gagcaaggtc tggatatcgc tgagatcgtc
     1621 gcgtttcatt gatgaatata tctcataggt ccatgcagat tatagcggct aatcatggga
     1681 aagggcaggg cctatagtcc ggcttcgtaa caggagacgc ctgatgtccg acctcgccca
     1741 gatcatccgc cgcgccggat cgcaaccgtc gcaaaccggc cccgccgcgt ggttcaccgg
     1801 aagcgtccgc atcgacccgc tgtttcccgc cgtcgcgccg tcgcgcgcgt ccggcgcgcg
     1861 ggtgaccttc gagccgggcg cgcgcaccca
//
LOCUS       DASVLL010000716         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_427330,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000716 DASVLL010000000
VERSION     DASVLL010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427330"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF402_11745"
     CDS             complement(<1..515)
                     /locus_tag="VF402_11745"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HEX7801019.1"
                     /translation="MVIERSAAPPVPGLLKSDLRPGERVLRWFEPSEALAPFVQQFWS
                     AQWAIAAGERRLQPILPFPCANLVVSGGCATLIGVMTRATAQRLEGVGCAFGAKLRPG
                     ALRAFGVERPSALRNRSVPAATLLHGAAADWPAGDIEACVRAVETCLLAQNPKHDPVS
                     LKADEIVRVAQA"
     gene            613..1215
                     /locus_tag="VF402_11750"
     CDS             613..1215
                     /locus_tag="VF402_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7801020.1"
                     /translation="MILYYSPKTRAFPVLWALEELGADYELKSLDLSRGEQGEAEYRR
                     INPMMKVPTLVDGDAIVSETGAILLYLAETLTGPDLLPPLGDPRRAACLKWLFFVGSN
                     LEPAMGERFQGWTPNPYYNGWGDFDRVRDAMAEALGHGPWLLGSQFTVADIYLAADLR
                     IAQAGQLIATDLQPFADYLARIKRRPAFIRAMQIDSPEAA"
     gene            complement(1278..1574)
                     /locus_tag="VF402_11755"
     CDS             complement(1278..1574)
                     /locus_tag="VF402_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7801021.1"
                     /translation="MAVNNAGTVGAFKPLAEITPEEWRIMLETNLTAAFLGARSQIPA
                     MLDRGGGAMIFISSFVGTSAGIPGMAAYGASKAALMGLVKGVAADYTAQGVTDD"
     gene            complement(1663..1758)
                     /locus_tag="VF402_11760"
                     /pseudo
     CDS             complement(1663..1758)
                     /locus_tag="VF402_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          339 a          631 c          615 g          347 t
ORIGIN      
        1 gcctgcgcga cgcgcacaat ctcgtcggcc tttaacgaaa ccgggtcgtg cttcggattc
       61 tgagcaagga gacaggtctc gacagcacgc acgcacgcct cgatatcccc ggcgggccaa
      121 tcggcggcgg cgccgtgcag aagcgtcgcc gcagggaccg accggttgcg cagcgcggat
      181 ggtctttcca cgccgaaggc cctcaaggcg ccgggccgca acttcgcccc gaacgcgcag
      241 cccacgcctt cgagccgctg cgcggtcgcg cgcgtcatca cgccgatgag ggtcgcgcat
      301 ccgcccgaaa cgacgaggtt ggcgcacgga aacggcagga tgggctgcaa acggcgctct
      361 ccggccgcga tcgcccattg cgccgaccag aattgctgaa cgaagggcgc cagcgcctcg
      421 gacggctcga accagcgcag gacgcgctcg cccggacgaa ggtcggattt gagaagccca
      481 gggacaggcg gggcggcgct ccgttcgatg accatgccgt cgatctttct ccgataggcg
      541 gttgcgggtt tttacaagac accgccgact gcgttcgcca tccttgcggc gtaaacgcca
      601 aaggagcgat gaatgatcct gtattacagc ccgaaaacgc gggcctttcc agtcttatgg
      661 gcgcttgagg aactcggcgc ggactatgaa ctcaaatcgc tcgatctgag ccggggcgag
      721 caaggcgagg ccgaataccg gcgcatcaac ccgatgatga aggttccgac gctcgtggac
      781 ggcgacgcta ttgtcagcga aaccggcgcg attctgctct atctcgcgga gacgctgacc
      841 ggcccggacc tgcttccgcc gctgggcgac ccccggcgcg ccgcctgtct gaaatggctg
      901 tttttcgtgg gctccaatct ggagccggcc atgggcgagc gctttcaggg gtggacgcca
      961 aatccctact ataacggctg gggcgacttc gacagggttc gcgacgccat ggccgaagcg
     1021 ctcggccatg ggccgtggct gctggggagc cagttcaccg tcgccgacat ctatctcgcg
     1081 gcggaccttc ggatcgcgca ggcgggccag ctcatcgcga cggatcttca acccttcgcc
     1141 gactatcttg cgcgtatcaa acgccggccc gcgtttatcc gcgccatgca gatcgattcg
     1201 cccgaggccg cgtaaaaacc ggagcgccgc gcagccaatt ccgctctgcg tggcgcgcct
     1261 tgtgacgtcc ttccatatca gtcgtctgtt acgccctgcg ctgtatagtc ggcggcgacg
     1321 cctttcacca agcccatcag ggccgctttg gaggcgccat aggcggccat gccggggatg
     1381 ccggcgcttg tgcccacgaa gctcgatatg aagatcatcg cgccgccgcc gcgatcgagc
     1441 attgccggaa tctggctgcg ggcgcccagg aaggcggccg taagattggt ctccagcatt
     1501 atccgccatt cttccggcgt gatctcggcc agcggcttga aggcgcctac ggtgccggcg
     1561 ttgttgaccg cgatgtcgag cccgccgaac ctgtccgtcg cgggtctcga tcagccgcgc
     1621 gtgggtttcg gcgtgaccga tctctcccgc catgacctcc gcgcggtcgc cattcttgcg
     1681 gatcgcgtca gcgacttcgt cgagccgggc cttacgccgt gcagtgagaa ccaccgacgc
     1741 tccgtgcgcg gcgaacatca acgctatggc gcggccgatg ccggaagacg cgccggtgac
     1801 gatggcgacc ttgccttcga gtcatgccgt agcttgtgta ggttcttata aagtcagatt
     1861 gaatggtgcc gcctttgttg atattcggcg cattggcaaa ccagtataaa atatcaggac
     1921 taactccggg ac
//
LOCUS       DASVLL010000717         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_536487,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000717 DASVLL010000000
VERSION     DASVLL010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536487"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..616)
                     /locus_tag="VF402_11765"
     CDS             complement(194..616)
                     /locus_tag="VF402_11765"
                     /inference="COORDINATES: protein motif:HMM:NF002616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX7801022.1"
                     /translation="MVTQYWLGVACAEHVRKGRAGGFMQLNHGKLAPLRRLSPGDGVV
                     YYSPSQRMGVRDGYQSFTAIGFVGPRPPYSGENGMFRRDVEWLAAAEHPIRPLLDWLD
                     FTQGENWGYQLRFGLLAIPEVDFDFLRHVMTQPALLSA"
     gene            complement(610..975)
                     /locus_tag="VF402_11770"
     CDS             complement(610..975)
                     /locus_tag="VF402_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7801023.1"
                     /translation="MSVKGSCHCGATRFEIAAAPEQVTSCTCTLCFKRGALWAYYQPD
                     QVKLETPSTTATYRWRTKTVKHHFCPHCGCTTYSESPDWSSGEPDFEHPRFAVNARLL
                     DEIDVAAIPVQIIDGRNGW"
     gene            complement(1030..>1596)
                     /locus_tag="VF402_11775"
     CDS             complement(1030..>1596)
                     /locus_tag="VF402_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX7801024.1"
                     /translation="LTGVLTDKVVGTDVASLPYGEMLHDHARWVDSLGREGKPSVFDR
                     ADLRGLGQIRGLNLAALSAKGAVFYGLDMQGVQLQGAHLEDSDLRNCNLRGADLRGAR
                     LIRARLDGADLTGARLGALKIDASRLLPTDLRDSRLRGANLSGADLQLARLDGADLSR
                     ANLEGAAMKLEALENATCVGLRGRLAVS"
BASE COUNT          280 a          571 c          482 g          263 t
ORIGIN      
        1 tggccaacgg ccttctggcc atcaccctga cgctggaaat ccccgaagcc aagaagccga
       61 agaccatcgc gatcaagacc gcccccgcct cggacgcgcc ggtcctgcaa gccgccgagt
      121 aaggccgctt ttttttcctt ctgtttctcc cgaacaaact gcgcgccccg gtcctccccc
      181 gccggggcgt tttttatgcg ctcagcaggg cgggctgcgt catgacatgg cgcaggaagt
      241 cgaaatcgac ctccgggatg gccagcagcc cgaaacgcaa ctgatacccc cagttctccc
      301 cctgcgtgaa gtccagccag tcgagcagcg gccggatcgg atgctccgcc gccgccagcc
      361 actcgacatc gcgccggaac atgccgtttt cacccgaata gggcggccgt gggccgacga
      421 aaccgatggc ggtgaagctc tgataaccgt cacggacgcc catccgctgc gacggcgaat
      481 aatagacgac gccgtcgccc ggcgacagac ggcgcagcgg cgccagcttg ccgtgattga
      541 gctgcatgaa gccgcccgcg cgtcccttgc gcacatgctc ggcgcaggcc acgcccaacc
      601 agtattgcgt caccatccgt ttctcccgtc gataatctgc accggaatcg ccgccacatc
      661 gatctcgtcg agcaggcgcg cattgacggc gaagcggggg tgttcgaaat ccggctcgcc
      721 cgacgaccag tcgggcgatt cggaataggt cgtgcaaccg caatgcggac agaaatgatg
      781 cttcaccgtc ttggtgcgcc agcgataggt cgcggtggtc gacggcgtct caagcttcac
      841 ctgatcaggc tgataatagg cccatagcgc accacgcttg aagcagagcg tgcaggtgca
      901 tgacgtgacc tgttccggcg cggcggcgat ctcgaaacgc gttgcgccgc aatggcagct
      961 tccctttacc gacatggctc tctcctctcg atccgaaccg agactagaca tgcctcatgt
     1021 cagtttttgt caggacacgg ccagccgtcc gcgcaggccc acacaggtgg cgttctccag
     1081 cgcctccagc ttcatggccg cgccttcgag attggcgcgc gacagatcgg ccccgtccag
     1141 ccgcgccagt tgcaggtcgg ccccgctcag attggcgccg cgcagacgtg aatcgcgcag
     1201 gtcggtcggc aacagccgcg aggcgtcgat cttcagcgcg cccagccggg cgccggtcag
     1261 gtccgccccg tccagccgcg cccggatcag ccgcgccccg cgcagatccg cgcccctgag
     1321 gttgcagttg cgcaggtccg aatcctccag atgcgcgccc tgcaactgca cgccctgcat
     1381 gtcgaggcca tagaacaccg cccccttggc cgacagcgcc gccaggttga ggccgcgtat
     1441 ctggcccagc ccgcgcaggt cggccctgtc gaagacggag ggcttgccct cgcggcccag
     1501 actgtcgacc catctggcgt ggtcgtgcag catctcgccg taaggcaggc tggccacgtc
     1561 cgtgccgacg accttgtcgg tcagcacgcc ggtcag
//
LOCUS       DASVLL010000718         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_373582,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000718 DASVLL010000000
VERSION     DASVLL010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373582"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1200
                     /locus_tag="VF402_11780"
     CDS             34..1200
                     /locus_tag="VF402_11780"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX7801025.1"
                     /translation="MDICEKYNIKTVHSSRTYDLVCEDGDREIAHELKTSGIAFSLHE
                     GAVLAAPNAVKTQDGRPYRVFTPFFKALQNTGVLHRPELPEPRAGAWPGPAVWPDSLR
                     IEDLALAPGGDETRPFDRFTPGERGARRALRRFLRDGLPGYAEGRDRPDLDLTSHLSP
                     HLRFGEISPRRILIELDKAVAANPHLMASAAKFRAELAWREFSYNLLHQQPRLHEINF
                     RNDFDAFPWRDDKDALRAWRCGETGYDLVDAGMKELRASGYMHNRVRMVAASFLVKHL
                     LIDWREGERWFWDSLLDADPANNPASWQWTAGCGADAAPYFRIFNPISQAAKVDPKGV
                     YRARWLGGDLDSYVKADKAGRPNADPRRRPIVDHDHARRRALDAYQNREPHEPD"
     gene            1187..2011
                     /locus_tag="VF402_11785"
     CDS             1187..2011
                     /locus_tag="VF402_11785"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX7801026.1"
                     /translation="MNQIDAPELHNLMADKAVKIVDGSWALDGTDMRALYAQDHIPGA
                     VFFDLDAISDRTSDLPHMAPAPEAFAAAVGQMGISDRDAVVVYDRQGLFSAARVWWTF
                     RLMGHKDVRVLRGGLPAWKAAGYATDASVSSPAPADYHAVLRHDLCIDRDQVLHGLRD
                     DDTLILDARPRARFDGAAPEPRAGLRSGHMPGSRNLPFSDLIRDGALLPKSELESVFR
                     DLDAGGKARLIATCGSGVTAAVISLALAETGREARLYDGSWAEWGREDAQTPVVKS"
     gene            complement(2022..2098)
                     /locus_tag="VF402_11790"
     tRNA            complement(2022..2098)
                     /locus_tag="VF402_11790"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2062..2064),aa:Arg,seq:tct)
BASE COUNT          369 a          697 c          701 g          384 t
ORIGIN      
        1 acgatcgcgg cgggaagcgc ccttctgacg ctattggata tatgcgaaaa atataacata
       61 aaaacagtgc attcttcgcg aacatatgat ctggtttgtg aagatggcga ccgcgagatc
      121 gcccacgaac tgaagacgtc cgggatagcg ttttcgctcc atgaaggcgc cgtgttggcg
      181 gcgccgaacg ctgtaaaaac ccaggatggc cgcccctacc gcgtctttac gcccttcttc
      241 aaggcgctgc aaaataccgg cgtcctgcat cggccggaat tgccggaacc gcgcgcgggc
      301 gcatggccgg gtccagccgt gtggcccgac tcgctgcgca tcgaggatct ggcgcttgcg
      361 cccggcggcg atgagacgcg gcctttcgac cgtttcacgc cgggcgaacg cggcgcgcgt
      421 cgggcgctgc ggcgattttt gcgcgacggg ctgcccggct atgccgaagg acgcgaccgg
      481 cccgatcttg acctgacctc gcatctgtcg ccgcacctgc gttttggcga gatcagtccg
      541 cgccgtattc tgatcgagct ggacaaggcg gtcgccgcca acccgcatct tatggcgtcg
      601 gcggccaaat ttcgcgccga actggcctgg cgcgagtttt cctacaatct gctccatcag
      661 cagccgcgcc tgcatgagat aaatttccgc aacgatttcg acgcttttcc gtggcgcgac
      721 gacaaggacg cgttgcgcgc ctggcggtgc ggcgaaaccg gttacgatct ggtcgacgcg
      781 ggcatgaaag agcttcgggc cagcggctat atgcacaacc gggtgcgcat ggtcgcggcc
      841 tcctttctgg tcaagcacct gctgatcgac tggcgcgagg gcgaacgctg gttctgggac
      901 agcctgctcg acgccgatcc ggccaataac ccggcaagct ggcaatggac cgccggttgc
      961 ggcgccgacg ccgcgcccta tttccgcatc ttcaacccga tttcacaggc ggcgaaagtc
     1021 gatccgaagg gcgtttatcg cgcccgctgg ctgggcggcg atctggacag ttacgtcaag
     1081 gctgataaag ctgggcgtcc caatgccgac ccgcgccgcc gcccgatcgt cgaccacgac
     1141 catgcccggc gccgcgcgct cgacgcctac cagaatcgtg aacctcatga accagattga
     1201 cgccccggaa ctgcataacc tgatggccga caaggccgtc aagatcgtcg acggcagttg
     1261 ggcgctcgac ggaaccgaca tgcgcgcgct gtacgctcag gatcatatac cgggcgccgt
     1321 ctttttcgat ctcgacgcca tcagcgaccg gaccagcgac ctgccgcaca tggcgccggc
     1381 gccggaagcc ttcgccgccg ccgtcggaca gatggggatc agcgaccgcg acgcggtcgt
     1441 cgtctatgac cggcaagggc tgttttcagc ggcgcgcgtc tggtggacgt tccggctgat
     1501 gggccataag gatgtgcggg ttctgcgcgg cggactgccc gcgtggaagg cggcgggcta
     1561 tgcgacggac gcctccgtct cctcgcccgc gccggccgat tatcacgccg tcttgcgtca
     1621 cgatctttgc atcgatcgcg atcaggtgct gcacggtttg cgggacgacg acacgctcat
     1681 cctcgacgcc cgtccgcgcg cgcgcttcga cggcgcggcg ccggagccgc gcgccggtct
     1741 ccgctcaggc catatgccgg gcagccgtaa tctgccgttt tccgacctga tccgggacgg
     1801 cgcgcttttg ccgaaatcgg aactggagag cgtcttccgt gatctggacg cgggcgggaa
     1861 ggcgcgcctg atcgccacct gcggctccgg cgtgaccgcc gccgtcatca gcctggccct
     1921 ggcggaaacc ggccgcgaag cgcgtcttta tgacggctcg tgggcggaat ggggccgtga
     1981 ggacgcgcag acgcccgtcg tgaaaagctg aaaaggccgc gtggcgactc cgacaggact
     2041 cgaacctgta accgtccgct tagaaggcgg atgctctatc cagttgagct acggagcctg
     2101 agggggcctc agtgggtcca gggttgcttg cggctgaacg cgaagttgtc g
//
LOCUS       DASVLL010000719         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_105587,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000719 DASVLL010000000
VERSION     DASVLL010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105587"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VF402_11795"
     CDS             <1..871
                     /locus_tag="VF402_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX7801027.1"
                     /translation="LFGASRVAPVDSYPNDLLATLKEVAPAGAGSDPTVVVLTPGAFN
                     SAYYEHSFLADLMGVELVEPMDLYVDDDRVWMKTTHGPRRVDVIYRRIDDDYLDPLTF
                     RPDSMLGVPGLFGAYRAGGVTICSAPGAGIADDKAIYLFVPDMIRFYLSETPILQNVP
                     TWRCALPDECAHVLAHLDELVAKEVHGSGGYGMLIGPRATRAEIEAYAARIRANPTDF
                     IAQPTLDLSTAPVLGHDAIVGRHVDFRPYCLVGKQMRLTPGGLTRVALTEGSLVVNSS
                     QGGGVKDTWVLED"
     gene            873..1808
                     /locus_tag="VF402_11800"
     CDS             873..1808
                     /locus_tag="VF402_11800"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX7801028.1"
                     /translation="MLSRTAENIFWMARYIERAESTARLIQMGQRMAILPGPIHREEW
                     RSVLRVTGCDGLIDGARAVGEADIVQLLLLDEGNPSSIRSCMTRARANGRAVRTALTQ
                     DMWEALNEDWRKLESYDAASARRHLPGLLDWVKTRASTFRGAAETGQIRNEGHDFLRI
                     GGALERAERTLRLLDVKYYVLLPETSVVGGARDHYQWTSVLHALSAARAYHHVYGGGD
                     YTPWRIADLLILNAQFPRSLSFCFGQIAWHLDRLAAAHGGRSAAQDVAAGLLSELRDR
                     ESGEIFEEGLHEFVQNALAVTNRLSREIAQAYHFA"
     gene            1818..>2147
                     /locus_tag="VF402_11805"
     CDS             1818..>2147
                     /locus_tag="VF402_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7801029.1"
                     /translation="MLLVVRHLTHMRYPHGAAGLALRLKLFPQSYDGQRVIDWHVEAD
                     GREMGPSIVGGYGERFAQWLAQGPRETLDIVAYGRVETENTAGIVKGLRRAPPPAIFL
                     RETALTRP"
BASE COUNT          332 a          750 c          725 g          340 t
ORIGIN      
        1 actgttcggc gccagccggg tggcgccggt cgattcctat cccaacgacc tgctggcgac
       61 gctgaaggag gtcgcgcccg ccggggcggg cagcgatccc accgtcgtgg tgctgacgcc
      121 gggcgcgttc aactcggcct attacgaaca tagctttctc gccgacctga tgggcgtcga
      181 actggtcgag ccgatggatc tttatgtcga tgacgaccgc gtgtggatga agacgacgca
      241 cggcccccgc cgggtcgacg tgatctatcg ccgcatcgac gacgactatc tcgatccgct
      301 gaccttccgc ccggattcca tgctgggcgt gccgggcctg ttcggcgcct atcgcgcggg
      361 cggggtgacg atctgctcgg cgcccggcgc cgggatcgcc gacgacaagg cgatctatct
      421 gttcgtgccc gacatgatcc gcttctatct ttcggagacg ccgatcctgc aaaacgtgcc
      481 gacctggcgc tgcgcgctgc ccgacgaatg cgcccatgtg ctggcgcatc tcgacgagct
      541 ggtcgccaag gaggtgcacg gatcgggcgg ctacggcatg ctgatcggcc ccagggcgac
      601 gcgcgccgaa atcgaggcct acgccgcgcg catccgcgcc aatccgaccg acttcatcgc
      661 gcagccgacg ctcgacctgt cgaccgcgcc ggtgctgggc cacgacgcga tcgtcgggcg
      721 ccacgtcgat ttccgcccct actgcctggt cggcaagcag atgcggctga cgcccggcgg
      781 gctgacccgc gtcgccttga ccgaaggctc gctggtggtc aactcgtcgc agggcggcgg
      841 cgtgaaggac acctgggtgc tggaagactg acatgctgag ccgcaccgcc gaaaacatct
      901 tctggatggc gcgctatatc gagcgcgccg aaagcaccgc gcggctgatc cagatggggc
      961 agcgcatggc gatcctgccc ggtccgatcc accgcgagga gtggcgctcg gtgctgcgcg
     1021 tcacgggctg cgacgggctg atcgacggcg cgcgcgcggt gggcgaggcc gatatcgtcc
     1081 agttgctgct gctcgacgaa ggcaatccgt cgtcgatccg ctcctgcatg acgcgcgcgc
     1141 gggccaacgg ccgcgcggtg cgcaccgcgc tgacgcagga catgtgggag gcgctcaacg
     1201 aggactggcg caagctggaa tcctacgacg cggccagcgc gcgccgccac ctgcccggcc
     1261 tgctcgactg ggtcaagacg cgcgcctcca cctttcgcgg cgcggcggaa accgggcaga
     1321 tccgcaacga gggtcacgac ttcctgcgca tcggcggcgc gctcgaacgc gccgaaagga
     1381 cgctgcggct gctcgacgtg aaatactatg tgctgctgcc cgaaacctcg gtggtcggcg
     1441 gcgcgcgcga tcattaccag tggacctcgg tgctgcacgc cctgtcggcg gcgcgcgcct
     1501 accatcacgt ctatggcggc ggcgactata cgccgtggcg gatcgccgat cttctgatcc
     1561 tcaacgcgca gtttccgcgc tcgctgtcct tctgcttcgg ccagatcgcc tggcacctcg
     1621 accggctggc cgccgcgcac ggcggacgca gcgcggcgca ggacgtcgcc gccggtctgc
     1681 tgtccgaact gcgcgaccgc gaaagcggcg aaatcttcga ggagggcctg cacgaattcg
     1741 tgcagaacgc gctcgccgtc accaaccgcc tgtcgcgcga gatcgcgcag gcctatcact
     1801 tcgcctgaag gttcccgatg ctgctggtcg tccgccatct cacgcacatg cgctacccgc
     1861 acggggcggc ggggctggcg ctgcgcctga aactgtttcc gcagagctat gacgggcaaa
     1921 gggtcatcga ctggcatgtc gaggcggacg ggcgcgagat gggtccctcc atcgtcggcg
     1981 gctatggcga gcgcttcgcg caatggctgg cgcaaggccc gcgcgagacc ctcgacatcg
     2041 tcgcctacgg ccgcgtcgag acggaaaaca ccgccggaat cgtcaagggc ctgcgccgcg
     2101 cgccgccgcc cgcgatcttc ctgcgcgaaa cggcgctcac ccgtccc
//
LOCUS       DASVLL010000720         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_321674,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000720 DASVLL010000000
VERSION     DASVLL010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321674"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..819
                     /locus_tag="VF402_11810"
     CDS             412..819
                     /locus_tag="VF402_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX7801030.1"
                     /translation="MIQPVLALIAWSMIMLVWLYARRVPFLVRYALNEVRLNSSDAVR
                     RMPPQAQWPADNYNNLMQQPTLFYALCLGIHVWGVSNLDLEYMAWLYVALRIMHSLVQ
                     STWNITTVRFCLFLASSGVLGVLCVRALFLIFA"
     gene            complement(823..>1798)
                     /locus_tag="VF402_11815"
     CDS             complement(823..>1798)
                     /locus_tag="VF402_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX7801031.1"
                     /translation="FIALEALRGACAYKGGRQYAAVCADMARQDFESPQQVRDFLDSR
                     LQVETIDPGAQKTGLLTGYFVPDYDARLTPDAEFSQPVRGRPSDLVYVDGAQMTPRQT
                     GTRIAARKTGGLYVPYYSRAEIEALPPDAATPYYMRPEDYFFMQIQGSGFLDLPDGTR
                     VYAAYAADNGLPFAGIAKPMVQQGLLPADQTSGDAIHAWLAAHRGPEAQNLMNLDPRY
                     IFFVIKPDQTEPLGATGLSLPPGSSIAVDPAYHDYGDLFWLDAHAGANGLDGAFPAYQ
                     RLVAALDTGGAIKGDARADLYVGHGARAGAEAGRIKHNLRLWRIVPAE"
BASE COUNT          289 a          570 c          620 g          319 t
ORIGIN      
        1 gcatggccat ctatatgggc ggcgggccgt ccctgatgta cgccgccgac gccgtgctgg
       61 ccttcgagca atatgccggc gcccagaagc cttaaagaaa aaaccgccgg cgcgcgatgg
      121 cggccggcgg tcagcaggca acggacggcg gcctcatggg ggaaagaccg cgacccggag
      181 aaaacccgtc cgacggaccg gggctgcggc gcggcggacg atgaaacctg cccggagccg
      241 gttggtttca atggagacaa ggtatcatgc tgcgcgagcc cgcgccagcg gcggctcttc
      301 acggttgcgt gacgcggccc gacggcaaaa gcgcggcggg cgcgcggcga aacgttaaaa
      361 attacggtta attcaacctt attttagcat ttgccgcgat aatcgcatcc gatgattcag
      421 cctgtcctcg ccctgatcgc ctggtcgatg atcatgctcg tctggcttta cgcccggcgg
      481 gtgccgtttc tcgtgcgcta tgcgctgaac gaggtgcggc tgaacagttc cgacgccgtg
      541 cgccgcatgc cgccgcaggc gcagtggccc gccgacaatt acaacaacct gatgcagcag
      601 ccgaccctgt tctacgcgct gtgtctgggg atccacgtct ggggggtcag caatctcgac
      661 ctcgaatata tggcgtggct ctatgtggcg ctgcggatca tgcattcgct ggtgcagtcg
      721 acctggaaca tcaccaccgt gcgcttttgt ttgtttctgg ccagttccgg cgtgctgggc
      781 gtgctgtgcg tgcgggcgct gttcctgatc ttcgcctgag gtctattccg ccgggacgat
      841 gcgccacagg cgcagattgt gcttgatgcg gcccgcttcg gcgcccgcgc gcgcgccgtg
      901 gccgacatag aggtcggcgc gcgcgtcgcc cttgatcgcg ccgccggtgt ccagcgccgc
      961 caccagccgc tgataggcgg gaaaggcgcc gtcgagaccg ttcgcgcccg catgggcgtc
     1021 cagccagaac aggtcgccgt aatcgtgata ggccggatcg acggcgatgc tcgaccccgg
     1081 cggcaggctc aggccggtcg cgcccagcgg ttcggtctgg tccggcttga tgacgaagaa
     1141 gatgtagcgc ggatcgagat tcatcaggtt ttgcgcctcc ggcccgcgat gcgcggccag
     1201 ccaggcgtgg atcgcgtcgc ccgaagtctg gtcggcgggc aaaagaccct gctggaccat
     1261 gggtttggcg atgccggcga acggcaggcc gttgtcggcg gcgtaggcgg cgtaaacgcg
     1321 cgtcccgtcc ggcaggtcga gaaagcccga cccttgaatc tgcatgaaga aatagtcttc
     1381 cggccgcata tagtagggcg tggccgcgtc gggcggcagg gcctcgatct cggcgcggct
     1441 gtagtaaggc acgtaaagcc cgcccgtctt gcgcgcggcg atgcgcgttc ccgtctggcg
     1501 cggcgtcatc tgcgctccgt cgacatagac gaggtccgac ggacggccgc gcaccggctg
     1561 cgagaattcg gcgtcgggcg tcaggcgcgc gtcgtagtcg ggcacgaaat agccggtcag
     1621 caggccggtc ttttgcgcgc ccggatcgat cgtctcgacc tgcaggcggc tgtcgaggaa
     1681 gtcgcgcacc tgttgcgggc tttcgaaatc ctgccgcgcc atgtcggcgc agacggctgc
     1741 atattgccgc ccgcccttat aggcgcaggc gccgcgcagg gcctccagcg cgatgaac
//
LOCUS       DASVLL010000721         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_212517,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000721 DASVLL010000000
VERSION     DASVLL010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212517"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VF402_11820"
     CDS             complement(<1..1406)
                     /locus_tag="VF402_11820"
                     /inference="COORDINATES: protein motif:HMM:NF004832.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7801032.1"
                     /translation="METEVLICGAGPSGLMLAIELARRGVPFLLIDKAPEPFSGSRGK
                     GLQPRTLEILDMLGLAGRIAALGGPYPPLRLYAEDGSHRVSTAVAYRAPSPAEPFGGP
                     LMLPQFMTEGVLREHLAGLGHAPRFGAELIAFRQGARDVRAKLRVDGRVETVRALYLV
                     GADGGRSFVRRTLQTGFPGKTLPVRAIVADVHLDGLSRDIWHRWGRDAESQVAVCPLM
                     GTDLFQVQAPVPPEPEPDMSAAAIEQLVRARSGRADIHIREVTWASAWTCNARLAERY
                     VQGRVMLMGDAAHVHPPTGGQGLNTSVQDAWNLGWKLAAVLRGGPDLLDTYESERRAA
                     AADVLGLSMRLLAEAAANADLRRDRAAQQLDLGYPDSRLSLGGGGRRLRAGDRAPDAP
                     CRWVTGQPTRLFDLYGPYWTLLAFGAETPAARAGLRCHRVGAELIDADGHIAAAYDAA
                     PGDLILIRPDGYVGARAT"
     gene            1485..1934
                     /locus_tag="VF402_11825"
     CDS             1485..1934
                     /locus_tag="VF402_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7801033.1"
                     /translation="MDPHIRQIHGALLDLVRVVNAPQPDAFLIQEAGVTLDRALFPLL
                     ARIGLRGPLGVVELAEMVGRDHTTVSRQIAKLERLGLVLRRAGARDRRVREAVLSPQG
                     EAVNAALNAARDRIFRQRLDTWPEGEKAELGRLLRKLADEALDWMKT"
     gene            complement(1941..2990)
                     /locus_tag="VF402_11830"
     CDS             complement(1941..2990)
                     /locus_tag="VF402_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7801034.1"
                     /translation="MRPVTIIHVAEKARVSVKTVSRVMNDEPNVTPATREKVLAAVKK
                     LGYSPSNAARRMGGARSWLLVSFNDRKLTIDNWRSARGNDWIDQMQHGAMLACERARY
                     HFLMELVDVEAEDMEAHITSILSRLRPDGVLLTPPSSDHPGVHKLLRRLGVPFARIGA
                     TRGKGLGHRVFMDERRAMQEIVRRLLGLGHRRIALITGSPAHASSLRKTEAFEKTLLK
                     AGVAQDDILIAPGDFTFESGLAATRALLDLRAPPTAIIGSNDEMTLAALNVAQQRGVK
                     TPDQLSLISFDDGSGVRFSHPAITAVHQPVAEMAAKAVELLVAAAAGQDMPDSSDTEL
                     PFTLVERASVARPPR"
BASE COUNT          498 a         1133 c         1118 g          462 t
ORIGIN      
        1 tcggtcgcgc gcgcgccgac atagccgtcg ggccggatca ggatcaagtc gcccggcgcc
       61 gcgtcgtagg cggcagcgat atggccgtcc gcgtcgatca gttcggcgcc gacgcgatga
      121 cagcgcaggc ccgcgcgcgc cgccggggtt tcggcgccga aggccagcag ggtccagtat
      181 ggcccgtaaa gatcgaacaa tcgcgtcggc tgacccgtga cccaccggca tggcgcgtcc
      241 ggcgcgcgat ccccggcgcg caggcggcgc ccgccgccgc cgaggctgag gcgcgagtcg
      301 ggatagccga gatcgagctg ctgggcggcg cggtcgcggc gcaggtcggc gttcgccgcc
      361 gcctccgcca gcagccgcat cgacaggccc agcacgtccg ccgccgccgc gcgccgctcc
      421 gattcgtagg tgtccaacag gtccggcccg ccgcgcagga ccgccgccag cttccagccg
      481 agattccacg cgtcctgcac cgaggtgttc agcccctgcc cgccggtcgg cggatgcacg
      541 tgcgccgcgt cgcccatcag catgacgcgc ccctgcacgt accgctccgc cagccgggcg
      601 ttgcaggtcc aggccgaggc ccacgtcact tcgcgaatgt gaatgtcggc gcgtccgctg
      661 cgcgcgcgca ccagttgctc gatcgcggcg gcggacatgt ccggctccgg ttccggcggc
      721 accggcgcct gcacctgaaa caggtcggtt cccatcagcg ggcagacggc gacctgcgat
      781 tccgcgtccc ggccccagcg atgccagatg tcgcgcgaca gcccgtcgag gtgcacgtcc
      841 gcgacgatgg cgcgcaccgg cagggtcttg ccgggaaagc cggtctgcaa ggtgcggcgc
      901 acgaagctgc gtccgccgtc ggccccgacc agatagaggg cgcgcacggt ttcgacgcgc
      961 ccgtccacgc ggagtttcgc ccgcacgtcg cgcgcgccct gccggaacgc gatcagctcg
     1021 gcgccgaaac gcggcgcatg gcccagcccg gccagatgct cgcgcagcac gccttcggtc
     1081 atgaattgcg gcagcatgag cgggccgccg aacggttcgg ccggggacgg cgcgcgatac
     1141 gcgaccgccg tcgaaacgcg atggcttccg tcctccgcat agaggcgcag cggcggatag
     1201 ggcccgccca gcgcggcgat acgcccggcc agccccagca tgtcgaggat ttcgagcgta
     1261 cgcggctgca accccttgcc gcgcgagccg ctgaacggtt cgggcgcctt gtcgatcagc
     1321 aggaacggga cgccgcgccg cgccagttcg atggccagca tcaggccgga aggccccgcg
     1381 ccgcaaatca ggacttcggt ttccatgtcg atcgcctttg tgtgtataat gcacatatat
     1441 cgcggcgctg gacgccgtca agcaaaaatg tgtatgatgc acacatggac ccgcatatcc
     1501 gtcaaattca cggcgcgctg ctcgacctcg tgcgcgtcgt caacgcgccg cagcccgacg
     1561 ccttcctgat tcaggaggcc ggggtgacgc tcgaccgggc cttgttcccg ctgctggcgc
     1621 gcatcgggct gcgcggcccg ctgggcgtgg tcgagctggc cgagatggtg gggcgcgacc
     1681 acaccacggt cagccgccag atcgccaagc tggagcgcct cggtctggtg ctgcgccgcg
     1741 cgggcgcgcg cgaccggcgc gtgcgcgaag ccgtcctcag cccgcagggc gaggcggtca
     1801 acgcggcgct gaacgcggcg cgcgaccgca tcttccgcca gcggctggac acatggccgg
     1861 aaggcgaaaa ggcggaattg ggccgcctgc tgcgcaagct ggccgacgaa gcgctggact
     1921 ggatgaagac gtaggcccga ttatcgaggc ggccgcgcga ccgaggcgcg ctcgacgagg
     1981 gtgaacggca gttcggtgtc ggagctgtcc ggcatatcct gcccggcggc ggcggccacc
     2041 agcagctcga ccgccttcgc cgccatctcg gccaccggct gatgcaccgc cgtgatcgcc
     2101 ggatgcgaaa agcgcacgcc ggagccgtcg tcgaagctga tcagcgacaa ctggtccggc
     2161 gtcttcacgc cgcgctgctg cgcgacgttc agcgccgcca gcgtcatctc gtcgttggag
     2221 ccgatgatcg cggtgggcgg tgcgcgcagg tccagcagcg cgcgcgtcgc ggccaggccc
     2281 gattcgaagg tgaagtcgcc cggcgcgatc aggatgtcgt cctgcgccac gcccgccttc
     2341 agcagggttt tctcgaacgc ctcggtcttg cgcagcgacg aggcgtgcgc cgggctgccg
     2401 gtgatcagcg cgatgcgccg gtggccgagg cccagaaggc ggcgcacgat ctcctgcatg
     2461 gcgcggcgct cgtccatgaa gacgcggtgg ccgaggccct tgccgcgcgt cgcgccgatg
     2521 cgcgcgaacg gcacgcccag acggcgcagc agcttatgca cgcccggatg gtcggagctg
     2581 ggcggcgtca gcagcacgcc gtcgggccgc agccgcgaca ggatggaggt gatgtgcgcc
     2641 tccatgtcct cggcctcgac atcgaccagc tccatgagga aatgataacg cgcccgctcg
     2701 caggccagca tggcgccgtg ctgcatctgg tcgatccagt cgttgccgcg cgccgagcgc
     2761 cagttgtcga tggtcagctt gcggtcgttg aacgacacca gcagccacga gcgcgccccg
     2821 cccatgcgcc gcgccgcgtt ggacgggctg tagccgagct tcttgaccgc cgccagcacc
     2881 ttctcgcgcg ttgcgggcgt gacgttcggc tcgtcgttca tcacccgcga cacggtcttg
     2941 accgaaaccc tggccttttc ggcgacgtga atgatggtga cggggcgcat aagggtccgc
     3001 gatactagag cgtttcccca aaaagtggct ccactttttg gataaggaag agctataaaa
     3061 caaagactta gagcgtcaat ctgatgcaat cagatcgaaa agcgctctaa gacgcgcgga
     3121 tactaggcgc agcccgcgcc ggtttcaagc cgcccgcgtc cccgccgtca gcgccgtcag
     3181 cagttcgcgg tggccgggca ggtggcgcag c
//
LOCUS       DASVLL010000722         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_266844,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000722 DASVLL010000000
VERSION     DASVLL010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266844"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..>2655)
                     /locus_tag="VF402_11835"
     CDS             complement(105..>2655)
                     /locus_tag="VF402_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7801035.1"
                     /translation="VMATIHPDDRESVAAALQRDLAGAGPCRAEYRVVRPDGSWRWVE
                     ASGVVSRDAGGRPLRFSGILIDTHERRQAEQALNDAHNQLRMAQRAGGVVGMWVWDLP
                     RQLVTGDEGFARVFGVAAGAARAGVPFSAIAPGVHPDDLPRLEAAVQKALAGELCQIA
                     YRVRDGAGWRWVDCTGKAEMDENGAPLSLSGIMVDIDARRRIEQALDESHQHLRLAQK
                     AGRIGVFVVPARGEVMQVSEAFCQIFGLPPAAQAPIDVVKACVLPEDRDARSTRADRA
                     EGATPPEAEYRIRRADDGELRWIYRRGEFVRDATGAPLHMLGIVQDITERKLAALRLK
                     ESEDYFNLMLDSVQDHAIITLDEGGRIVLWNPGAQEIFGYKAGEAVGQGIELIFTEED
                     RAVDAPRAELAAAAVHGRTHDERWHRRRNGERFYTSGSMTAMRDGEGRVCGFIKIARD
                     MTEQQKAQQALMEARNAAEAANLAKSEFLANMSHEIRTPMNAIIGLSALLAKSQPLSQ
                     RQGAYIRTLQTSADTLLDLINDLLDVAKIEAGAIELERVPFTFSRLAQEIGEMLGVPA
                     RDKGLALRIDAGAVADRIYLGDPTRLRQIVMNLGSNAVKFTETGEVEIVFLREPMADG
                     RDLVRIRVRDTGVGIAEDKVAAIFQKFVQADSGINRKYGGTGLGLAITRTLCEIMGGE
                     ISLRSAPGEGSVFEAAAPLEIAEAAQAAQADLSLAARAETCVVAGTPPEVLLVEDHEP
                     NILVATAFLQEFGYAVDVAVSGREAYDRLEHRAYAAVLMDVQMRDVNGLDATRMIRER
                     EAREGRAPVRIIGMTAHALSGDRERCLAAGMDDYISKPFRPDDLRALLQNE"
BASE COUNT          367 a          967 c          878 g          443 t
ORIGIN      
        1 gcgttgacct tggcatgatc cgtcccgtcg atgtcgcgca tccgcattcc gcccaccatt
       61 tgtttccgat ggagggacat taggatatac aacttaaaat tgttttattc attttgcaac
      121 aacgcgcgca ggtcgtccgg acggaacggt ttggagatgt agtcgtccat gcccgccgcc
      181 aggcagcgct cgcggtcgcc cgacagggcg tgcgccgtca tgccgatgat gcgcacgggc
      241 gcgcggcctt ccctcgcctc gcgctcgcgg atcatgcgcg tggcgtccag cccgttgacg
      301 tcgcgcatct gcacgtccat cagcacggcg gcgtaggcgc gatgctccag cctgtcgtaa
      361 gcctcgcggc cgctgacggc gacgtcgacc gcatagccga actcctgcaa gaaggccgtg
      421 gcgaccagaa tgttcggctc gtgatcctcg accagcagca cctcaggcgg cgtcccggcc
      481 acgacgcagg tttcggcgcg cgccgccagc gacaggtcgg cctgcgccgc ctgcgccgcc
      541 tcggcgatct ccagcggcgc ggcggcctcg aacaccgagc cttcgcccgg cgcgctgcgc
      601 aggctgatct cgccgcccat gatctcgcac agggtccggg tgatggccag ccccagcccc
      661 gtgccgccgt atttgcggtt gatgccggaa tcggcctgca cgaacttctg gaagatcgcg
      721 gcgaccttgt cctcggctat gccgacgccg gtgtcgcgca cgcggatgcg cacaaggtcg
      781 cgcccgtccg ccatcggctc gcgcaggaac acgatctcga cctcgcccgt ctcggtgaac
      841 ttgaccgcgt tggagccgag attcatcacg atctgccgca gccgcgtcgg atcgcccaga
      901 tagatgcggt ccgccaccgc cccggcgtcg atgcgcaggg ccagcccctt gtcgcgcgcc
      961 ggcacgccca gcatttcgcc gatctcctgc gccaggcggc tgaaagtgaa cggcacgcgc
     1021 tccagctcga tggcgccggc ctcgatcttg gccacgtcga gcaggtcgtt gatcaggtcc
     1081 agcagcgtgt cggcgctggt ttgcagggtg cggatatagg cgccctgccg ctggctcagc
     1141 ggctggctct tggccagcag cgccgacagg ccgatgatgg cgttcatcgg cgtgcggatc
     1201 tcgtggctca tattggccag gaattcgctc ttggccagat tggcggcctc ggcggcgttg
     1261 cgcgcctcca tcagggcctg ctgcgccttc tgctgctcgg tcatgtcgcg cgcgatcttg
     1321 atgaagccgc acacccgccc ttcgccgtcg cgcatcgcgg tcatcgagcc ggaggtgtag
     1381 aagcgctcgc cgttgcggcg ccggtgccag cgttcgtcgt gggtgcggcc atgcacggcg
     1441 gcggcggcca gctcggcgcg cggcgcgtcg acggcgcggt cctcctcggt gaagatcagc
     1501 tcgatcccct gcccgaccgc ttcgcccgcc ttgtagccga agatttcctg cgcgccggga
     1561 ttccacagca cgatgcgccc gccttcgtcc agcgtgatga tggcgtggtc ctgcacgctg
     1621 tccagcatca gattgaaata gtcctcgctt tccttcagcc gcagcgccgc cagcttgcgc
     1681 tcggtgatgt cctgcacgat gcccagcatg tgcaggggcg cgcccgtggc gtcgcgcacg
     1741 aattcgccgc gccggtaaat ccagcgcagt tcgccgtcgt cggcgcggcg gatgcggtat
     1801 tcggcttccg gcggcgtggc gccttcggcg cggtcggcgc gcgtggagcg cgcgtcgcgg
     1861 tcctcaggca gcacgcacgc cttgacgacg tcgatgggcg cctgcgcggc gggcggcagg
     1921 ccgaaaatct ggcagaaggc ttccgagacc tgcatgacct cgccccgcgc cggaacgacg
     1981 aaaacgccga tccggcccgc cttctgcgcc agccgcagat gctgatggct ctcgtcgagc
     2041 gcctgttcga tgcggcggcg cgcgtcgata tcgaccatga tgccgctcag gctgagcggc
     2101 gcgccgtttt catccatctc cgccttgccg gtgcagtcca cccagcgcca gcccgcgccg
     2161 tcgcgcaccc ggtaggcgat ctggcacaac tcgcccgcca gcgccttctg caccgccgcc
     2221 tccagccgcg gcagatcgtc gggatgcacg cccggcgcta tggcggaaaa cggcacgccc
     2281 gcgcgcgccg ccccggccgc gacgccgaag acgcgcgcga agccttcgtc gccggtcacc
     2341 aactggcgcg gcaaatccca gacccacatg ccgaccacgc cgcccgcgcg ctgggccatg
     2401 cgcaactggt tgtgggcgtc gttcagcgcc tgctcggcct gacggcgctc atgcgtgtcg
     2461 atcaggatgc cggagaagcg cagcggcctg ccgccggcgt cgcggctgac gacgccgctg
     2521 gcctcgaccc agcgccagct tccgtccggg cgcacgacgc ggtattcggc gcggcacggc
     2581 cccgcgcccg ccagatcgcg ctgcaaggcc gccgcgacgg attcgcggtc gtccgggtgg
     2641 atcgtcgcca tgacc
//
LOCUS       DASVLL010000723         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_106220,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000723 DASVLL010000000
VERSION     DASVLL010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106220"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="VF402_11840"
     CDS             complement(<1..1436)
                     /locus_tag="VF402_11840"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HEX7801036.1"
                     /translation="MAAPASSRLSIASGFATDVGKRDDNQDFAALYLDEDASRHHGAV
                     AALADGVGGRKGGRVAAELSVRTFIDDYLGQSDILPPRKTAARALESINAWLYREGQK
                     NETLKGMACAFTGVVLKGRRLHSFHVGDTRLYRLRDGQLERLTQDHRPENAGGDATLL
                     SRAVGAEEAVRIDYGVTDLAPHDRLMLCSDGVHGMLSDTDIGLILDARAAPEETAQRL
                     VHTAIARGGDDNASAVVIDVIDLPPAAIEDLDAAIAGLPMIEPPQVGDVIDGFRLEKT
                     LADGLYSRVFRARDLKETRLAHQAVLLKFPKPHTIGAEASARSAFMREAWVASRVQHI
                     WVGSVLQLAPGRQTCLYIAQPFYAGETLEHRLTHPPKIGLKEGVGVAVKLAKALGSLH
                     RAGVIHRDVKPENIILTGDGGFKLVDLGVSRIPNMDKDTVFVPGTPSYKAPEQFEGRP
                     GDERSDIFAMGVTLYRMFTGAYPFGEIET"
     gene            complement(1538..2278)
                     /locus_tag="VF402_11845"
     CDS             complement(1538..2278)
                     /locus_tag="VF402_11845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7801037.1"
                     /translation="MTQGIENKIVVITGASSGLGEAAARLLTAQGAKVVLGARRLDRL
                     QGLAAELGLDERAVARTDVTDAAQVAALVERALELHGRIDVMLNNAGLMPHSPLERGK
                     IADWDRMIDVNIKGVLYGVAAALPHMRAQRSGHIINVSSVAGHKVGAGGAVYAATKHA
                     VRVISEGLRQEVKPYNIRSTVISPGAVDTELPGSVTEPDIAADVRARYAGWAIPADSF
                     ARCVAFAMAQPDAVDVNEILFRPTAQAY"
     gene            complement(2291..2716)
                     /locus_tag="VF402_11850"
     CDS             complement(2291..2716)
                     /locus_tag="VF402_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7801038.1"
                     /translation="MSDLAQIVRRAGSQPSQSGPAAWFTGRVRIDPLFPAVAPSRASG
                     ARVTFEPGARTHWHTHPLGQNLIVLSGVGWTQCEGGPKREIRPGDVVSCPCGKRHWHG
                     AAQTTAMSHIAVTETLDGSPVTWLEPVADADYLAPVAED"
     gene            2811..>3249
                     /locus_tag="VF402_11855"
     CDS             2811..>3249
                     /locus_tag="VF402_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7801039.1"
                     /translation="MKRDDLSDIQTFLTVVREGGFTRAAAKMGVSPSALSHSLRALEA
                     RLGVRLLTRTTRSLAPTEAGERLIRNVGPHLDGIAAELAALGELRDKPAGTIRITSGE
                     HSAETILWPKLAPLLRAYPDIRVEITVDYGLTDIVAERFDAGVR"
BASE COUNT          484 a         1106 c         1120 g          539 t
ORIGIN      
        1 gtctcgatct cgccgaacgg ataggcgccg gtgaacatgc ggtagagggt cacgcccatg
       61 gcgaagatgt cggagcgttc atcgcccggt cgcccctcga actgctccgg cgccttgtag
      121 ctgggcgtgc cgggcacgaa gaccgtgtcc ttgtccatat ttgggatgcg cgatacgccg
      181 aggtccacca gcttgaagcc gccgtcgccg gtcaggatga tgttctccgg cttgacgtcg
      241 cggtggatga cgcccgcccg atgcagcgag ccgagcgcct tggccagctt gaccgccacg
      301 ccgacgccct ccttcaggcc gatcttcggc ggatgggtca ggcgatgctc cagcgtctcg
      361 cccgcgtaga agggctgggc gatgtagagg caggtctgcc gccccggcgc cagttgcagc
      421 accgagccga cccagatgtg ctgcacgcgc gaggcgaccc aggcctcgcg catgaaggcc
      481 gaacgcgccg acgcctcggc gccgatggtg tgcggcttgg ggaatttcag cagcaccgcc
      541 tgatgcgcga ggcgcgtctc cttcaggtcg cgggcgcgga acacacgcga ataaagcccg
      601 tcggccaatg tcttttccag acggaaaccg tcgatgacgt cgccgacctg cggcggctcg
      661 atcatcggca gcccggcgat ggcggcgtcg agatcctcga tcgcggcggg cggcaggtcg
      721 atcacgtcga tgaccacggc gctggcgttg tcgtcgccgc cgcgcgcgat ggccgtgtgg
      781 accagccttt gcgccgtctc ctccggcgcg gcgcgcgcgt cgaggatcag gccgatatcg
      841 gtgtcgctca acatgccgtg gacgccgtcc gaacacagca tcagccgatc gtgcggcgcc
      901 aggtcggtga cgccgtaatc gatgcgcacc gcctcctcgg cgccgaccgc gcggctcagc
      961 agggtggcgt cgccgcccgc gttttccggg cggtgatcct gcgtcagccg ttcgagctga
     1021 ccgtcgcgca gccggtacag gcgcgtgtcg ccgacatgga agctgtgcag ccgccgcccc
     1081 ttcagcacca cgccggtgaa ggcgcacgcc atgcctttca gcgtctcgtt cttctggccc
     1141 tcgcgataga gccaggcgtt gatgctttcc agcgcccgcg ccgccgtctt gcgcggcggc
     1201 aggatgtcgc tctggcccag atagtcgtcg atgaaggtgc gcaccgacag ctccgccgcg
     1261 acgcgcccgc ccttgcgtcc gccgaccccg tcggccagcg ccgccaccgc gccgtgatgg
     1321 cggctggcgt cctcgtcgag atagagggcg gcgaaatcct gattgtcgtc gcgtttgccc
     1381 acatcggtgg cgaaaccgga agcgatgctc aggcgggaag atgcgggcgc ggccatgcct
     1441 taagttgtga ccgaggttgc gcgtttcgta aatggccgcg atgctgcgcc gcaaacatta
     1501 ccgcccggcg tggcttttat gttcagcccg cctgcgttca ataggcctgc gcggtcgggc
     1561 ggaacaggat ctcgttgacg tcgaccgcgt ccggttgcgc catcgcgaag gcgacgcagc
     1621 gcgcgaacga gtccgccggg atcgcccagc cggcgtagcg ggcgcgcaca tcagcggcga
     1681 tgtcgggctc ggtgacgctg ccgggcagtt cggtgtcgac cgcgcccggc gaaatgacgg
     1741 tggaacggat gttatacggc ttgacctcct gccgcagccc ttcggaaatg acgcgcaccg
     1801 cgtgcttggt ggcggcgtaa accgcgccgc ccgccccgac cttgtgcccg gcgaccgagg
     1861 acacgttgat gatatggccg ctcctttgcg cccgcatgtg cggcagggcg gcggcgacgc
     1921 cgtacagcac gcccttgata ttgacgtcga tcatgcggtc ccagtcggcg atcttcccgc
     1981 gctccagcgg cgaatgcggc atcaggcccg cattgttgag catcacgtcg atgcggccat
     2041 gaagttccaa cgcccgttcg accagcgccg cgacctgcgc cgcgtcggta acgtcggtcc
     2101 gcgcgacggc ccgctcatcg aggccaagtt cggcggccag cccctgtagc cggtcgaggc
     2161 ggcgcgcgcc caggacgacc ttcgcgccct gcgccgtcaa cagccgcgcg gcggcctcgc
     2221 ccagcccgct cgacgcgccg gtgatgacga cgatcttatt ctctatgcct tgggtcattg
     2281 tgaagtctcc tcaatcttcg gcgacgggcg ccagatagtc ggcgtcggcg accggctcca
     2341 gccatgtgac gggcgatccg tccagcgttt cggtgacggc gatatggctc atggccgtgg
     2401 tttgcgccgc gccgtgccag tggcgtttcc cgcacgggca ggacacgacg tcgccggggc
     2461 ggatttcgcg cttggggccg ccttcgcact gcgtccagcc gacgccggac aggacgatca
     2521 ggttctggcc cagcggatgg gtgtgccagt gggtgcgcgc gcccggctcg aaggtcaccc
     2581 gcgcgccgga cgcgcgcgac ggcgcgacgg cgggaaacag cgggtcgatg cggacccgtc
     2641 cggtgaacca tgcggcgggg ccggattgcg acggttgcga tccggcgcgg cggacgatct
     2701 gggcgaggtc ggacatcgag cgtctcctgt tacagagccg gactataggc cctgcccttt
     2761 tccgtgatta gacgctataa tctgcatgag cctgtgagat atattcatca atgaaacgcg
     2821 acgatctcag cgatatccag accttcctca ccgtcgtgcg cgaaggcggc ttcacgcgcg
     2881 cggcggcgaa gatgggcgtg tcgccctcgg ctctgagcca cagcctgcgc gcgctggagg
     2941 cgcggctggg ggtgcggctt ctgacgcgca ccacgcgcag cctggcgccg accgaggccg
     3001 gggagcggct gatccgcaac gtcggcccgc atctcgacgg catcgccgcc gaactggcgg
     3061 ccttgggcga actgcgcgac aagcccgccg ggacgatccg catcacctcg ggcgaacatt
     3121 ccgccgagac gatcctgtgg ccgaaactgg cgcccttgct cagggcctat cccgatatcc
     3181 gcgtggagat cacggtggat tacggcctga ccgacatcgt ggccgagcgc ttcgacgcgg
     3241 gcgtgcggc
//
LOCUS       DASVLL010000724         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_322353,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000724 DASVLL010000000
VERSION     DASVLL010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322353"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VF402_11860"
     CDS             <1..1142
                     /locus_tag="VF402_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX7801040.1"
                     /translation="TKRWFGGAQGSGIPQVIAACEIVRHNPEKAQNLVSLRIAAGKIA
                     MLILGLACGAPIGREGPTVQVGASLMRLTGRLGGIVQNESALLLAGGAAGVAAAFNTP
                     LAGIVFAIEELGKSFEQRTSGTVILTVMLAGVASLGLMGDYTYFGRHTTHIEGWTVIL
                     AVLVCGVGGGLLGGGFSRATIFGLKRLGTLFGGWTKQHPILFAAGCGLVLAVIGVLSG
                     GAAFGTGYSDARDLLAGQQHVAHGYGLLRVAAALLMAVSGIPGGLFAPSLSAGAGVGA
                     DLHLMVPIVPLATMALLGMVGYFSGVVQSPLTAFVIVMEMTDDHDMVLPLIATSLLAY
                     SVSRLICPHPLYHSLAQNFLKQARADQRAEEAAKAGDQKSGDQAA"
     gene            complement(1130..2200)
                     /locus_tag="VF402_11865"
     CDS             complement(1130..2200)
                     /locus_tag="VF402_11865"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEX7801041.1"
                     /translation="MPAFPAPLPLHPVRRLRAALAGAQTVSRLERLRAALGGGFGIAI
                     TALICLTTAKAAHLAPWLVAPVGASAVLVFAVPSSPLAQPWSVIGGNTVSAIAGLLAC
                     NLIPDPVLAASAAVGMAIAAMFGLRCLHPPGGAMALLVVLTATRDPLFALFPAMTNSA
                     LLVGAGILYNTLTRKPYPHVQPARAAETSGLLRITPADMAAALAEHDEVLDIAADDLA
                     RLLERSELRAWRRLAGHRTCADIMTVPVHAVRPGVRLRHAEALMQRYDIKILPVTDRR
                     RRVLGLVVRDDLMRSREGAATAKDVMRRDYVAARTSDSLDDLMPLFSRGDRRHVLVLD
                     AQDRLAGVISASDTMRALYHAA"
     gene            complement(2378..3778)
                     /gene="creD"
                     /locus_tag="VF402_11870"
     CDS             complement(2378..3778)
                     /gene="creD"
                     /locus_tag="VF402_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEX7801042.1"
                     /translation="MSDTPTPYKPGLISRSRGAKLLIVCCLAVLMSIPAGFVFLLLHD
                     RSHRAEEVADEIGGLMGGPQTFMGPVISVPYTAPVRGGDGDAVKVESGNLIVFPVSGD
                     AQVTSKSEVRSRSLFRVPVYAARLNFTARFDLSQVASPVPNATLDWGHARLMTGASDS
                     RGARSDIVAHVSGPGGAQALTLAPDAGAASMDGGDKFDLFSASLDRLDLTQPLDVDLG
                     MNFTGAQKIGVLAFAKSTTVAIGGDWNWPSFGGGFLPATRAFHHDGAQPLMAGEQNMA
                     SGFKATWSVPFVARGLPPVITQAGLSSLDKSELSVAFVEPTNPYQNVGRSLKYALLFV
                     GLVFLTFFLFESLTRTELHPAQYILIGLAQITFYLLLLSLAERIGFDAAFAAAAAATV
                     GLISAYAGMVFRSLWRGVAALVVFGLLYGLIYILMRMEDYALLVGAASAFLTIAAVMI
                     LTRNLNWYGGREGERG"
     gene            complement(3875..4195)
                     /locus_tag="VF402_11875"
     CDS             complement(3875..4195)
                     /locus_tag="VF402_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7801043.1"
                     /translation="MAKITYIEHNGTEHVVEVRNGQSVMEGAVKNNIPGIDADCGGAC
                     ACATCHVYVDPAWTDKTGTASVMEESMLDFANELQPNSRLSCQILVNDDMDGLIVRMP
                     ADQH"
BASE COUNT          691 a         1375 c         1509 g          649 t
ORIGIN      
        1 tgacgaagcg ctggttcggc ggcgcgcagg gatcgggcat cccgcaggtc atcgccgcct
       61 gcgaaatcgt gcgccataac ccggaaaagg cgcagaacct cgtctcgctg cgcatcgcgg
      121 ccggcaagat cgccatgctg atcctggggc tggcgtgcgg cgcgccgatc gggcgtgaag
      181 gcccgaccgt gcaggtcggc gcctcgctga tgcgcctgac cggccgtctc ggcgggatcg
      241 tacagaacga atcggcgctg ctgctggcgg gcggcgcggc gggcgtggcg gcggccttca
      301 acacgccgct ggccgggatc gtcttcgcta tcgaggaact gggcaagtcg ttcgagcagc
      361 gcacctccgg cacggtgatc ctgaccgtga tgctggccgg ggtcgcctcg ctgggcctga
      421 tgggcgacta cacctatttc gggcggcaca cgacgcatat cgagggctgg acggtcattc
      481 tggccgtgct ggtctgcggc gtcggcggcg ggctgctggg cggcggtttc tcgcgcgcga
      541 cgattttcgg cctgaagcgc ctcgggacgc tgttcggcgg ctggaccaaa cagcatccga
      601 ttttgttcgc cgccgggtgc gggctggttc tggccgttat cggcgtcctg tcgggcggcg
      661 cggcgttcgg cacgggctac agcgacgcgc gcgacctgct ggccggtcag cagcatgtgg
      721 ctcacggcta tggcctgctg cgcgtggcgg cggcgctgct catggcggtg tccggcatac
      781 cgggcggctt gttcgcgccg tccctgtcgg cgggcgcggg cgtcggcgcc gacctgcacc
      841 tgatggtgcc gatcgtgccg ctggccacga tggcgctgct gggcatggtc ggctactttt
      901 ccggcgtggt gcagtcgccg ctgaccgcct tcgtcatcgt catggagatg accgacgatc
      961 acgacatggt gctgccgctg atcgcgacct cgctgctggc ctattcggtg tcgcgcctga
     1021 tctgcccgca cccgctctat cacagtctgg cgcagaactt cctcaagcag gccagagccg
     1081 atcagcgcgc cgaagaggcg gcgaaggcgg gtgatcagaa gagtggcgat caggcggcgt
     1141 gatacagcgc gcgcatggtg tccgaggcgc tgatcactcc ggccagccgg tcctgcgcgt
     1201 ccagcaccag cacgtgccgc cggtcgccgc ggctgaacag cggcatgagg tcgtccagtg
     1261 aatcgctggt ccgcgcggcg acatagtcgc ggcgcatgac gtctttagcg gtcgccgcgc
     1321 cttcgcgcga ccgcatcagg tcgtcgcgca ccaccagccc cagcacgcgg cgccgccggt
     1381 cggtcacggg caggatcttg atgtcgtagc gctgcatcag cgcctcggcg tggcgcaggc
     1441 gcacgccggg ccgcaccgca tgcaccggaa ccgtcatgat gtcggcgcag gtccgatgtc
     1501 cggccagccg ccgccacgcg cgcagttcgc tgcgctccag cagccgcgcc agatcgtcgg
     1561 cggcgatgtc cagcacctcg tcatgctcgg ccagggcggc ggccatgtcg gcgggcgtga
     1621 tgcgcaaaag ccccgacgtc tcggcggcgc gcgcgggctg gacatgcgga tagggcttgc
     1681 gcgtcagggt gttgtagagg atgcccgcgc cgaccagcag cgccgaattg gtcatggcgg
     1741 ggaacagcgc gaacagcgga tcgcgcgtgg cggtcagcac gaccagcagc gccatcgcgc
     1801 cgcccggcgg atgcaggcag cgcaggccga acatggcggc gatggccatg ccgacggcgg
     1861 cgctggcggc gaggaccgga tcggggatca ggttgcaggc cagcagcccg gcgatggcgg
     1921 aaaccgtatt gccgccgatc accgaccacg gctgcgccag cggcgaggac gggacggcga
     1981 acaccagcac ggcgctggcc cccaccggcg cgaccagcca cggcgccaga tgcgcggcct
     2041 tcgccgtcgt caggcagatc agggcggtga tcgcgatgcc gaagccgccg cccagcgccg
     2101 cgcgcaaccg ttcgaggcgg ctgacggttt gcgcgcccgc cagagcggcg cgcagacggc
     2161 ggacggggtg caggggcagg ggggcgggga aagcgggcat gaagatacgg ggtgcacggg
     2221 ggatacaagc ctcatgccga aaagtggtcg ccacttttcg gatatacatg aggcgtcaag
     2281 gaaaactccg ggccggtcgc gctggcgaac ggcccggagg caggcttgta accgccagcg
     2341 cccgtgagga caaagctatt tattctgcac cgcagcatta gccgcgctcg ccctcgcgtc
     2401 ctccgtacca gttgaggttg cgcgtcagga tcatcacggc ggcgatggtc aggaaggccg
     2461 aggccgcgcc gactagcagc gcatagtcct ccatgcgcat caggatatag atcaggccgt
     2521 acaaaaggcc gaacacgacc agcgccgcga cgccgcgcca caggcttctg aacaccatgc
     2581 ccgcataggc cgagatcagc cccaccgtcg cggcggcggc ggcggcgaag gcggcgtcga
     2641 agccgatgcg ctcggccagc gacagcagca gcaggtagaa ggtgatctgc gccagcccga
     2701 tcaggatgta ctgcgccgga tgcagctcgg tccgcgtcag gctctcgaac aggaagaagg
     2761 tcaggaagac gaggccgacg aacagcagcg cgtatttcag cgagcgcccg acgttctgat
     2821 acggattggt cggctcgacg aaggcgaccg acagttccga cttgtcgagc gaggacagac
     2881 cggcctgggt gatcaccggc ggcaggccgc gcgccacgaa cggcacggac cacgtcgcct
     2941 tgaagcccga cgccatgttc tgctcgcccg ccatcagcgg ctgggcgccg tcgtgatgga
     3001 aggcgcgcgt cgcgggcagg aagccgccgc cgaaggacgg ccagttccag tcgccgccga
     3061 tggcgaccgt ggtcgacttg gcgaaggcca aaacgccgat cttctgcgcg ccggtgaaat
     3121 tcatgccgag atcgacatcg agcggctgcg tcaggtcgag gcggtcgagc gaggccgaga
     3181 acaggtcgaa cttgtcgccg ccgtccatgc tggccgcgcc ggcgtcgggc gccagggtca
     3241 gggcctgagc gccgcccggc cccgacacat gcgcgacgat gtcgctgcgg gcgccgcgcg
     3301 aatcggacgc gccggtcatc agccgcgcat ggccccagtc gagcgtggcg ttcggcacgg
     3361 gcgaggcgac ctggctgagg tcgaagcgcg ccgtgaagtt cagccgggcg gcatagaccg
     3421 gcacgcggaa cagcgagcgc gagcgcacct cgcttttcga cgtgacctgc gcgtcgccgc
     3481 tgaccggaaa gacgatcaga ttgccgctct ccaccttgac ggcgtcgccg tcgccgccgc
     3541 ggaccggcgc ggtgtagggc acgctgatca ccggccccat gaaggtctgc ggcccgccca
     3601 tcaggccgcc gatctcgtcg gccacctctt cggcgcggtg gctgcggtcg tgcagcaaga
     3661 gaaagacgaa ccccgccggg atcgacatca gcacggccag acagcagacg atcaacagct
     3721 tggcgccgcg cgaacggctg atcaggccgg gtttgtaagg cgtaggcgta tcggacatgt
     3781 catccccctt gtttcaggac ggatgagtcg tatcacgcgc gcgggctgtg ggcaatcaaa
     3841 atttgaacga tgttcaaatt tagcgctggc aaatttagtg ctgatcggcg ggcatgcgca
     3901 cgatcaggcc gtccatgtcg tcattgacga ggatctggca cgacaggcgc gaattgggct
     3961 gcaactcatt ggcgaagtcg agcatcgact cctccatcac cgaggcggtc ccggtcttgt
     4021 ccgtccacgc cggatcgaca tagacgtggc aggtggcgca ggcgcaggcc ccgccgcaat
     4081 cggcgtcgat gccggggatg ttgttcttga ccgcgccttc catcacgctc tggccgttgc
     4141 ggacctcgac gacgtgttcg gttccgttgt gttcgatata ggtgatcttg gccatgatat
     4201 gcgtttcgtt tctgacggat cgaa
//
LOCUS       DASVLL010000725         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_428333,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000725 DASVLL010000000
VERSION     DASVLL010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428333"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF402_11880"
     CDS             <1..474
                     /locus_tag="VF402_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7801044.1"
                     /translation="RLPLPAWARPVALAAGVVLGLFAAGLPRAPLTPVTSAAPAVAGL
                     RQPFDVDALAQLRAQGRPVLVDLTAAWCVTCKVNERLALSTAKFEKALKATGTVYMVG
                     DWTHQDAAITRYLSLYGRSGVPLYVYYGAHDAAPRILPQILNADEVVRVLEAGAR"
     gene            complement(778..1338)
                     /locus_tag="VF402_11885"
     CDS             complement(778..1338)
                     /locus_tag="VF402_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801045.1"
                     /translation="MDCSIALALMLRGAAPADLVAPACPAAIVRVAEGQPVEGQPAED
                     SANRPATDPISHEPLYAGLVQDANALRAETQDFSKAPSLALRDAPRFQTYLQRLQNLS
                     AGDLKGHFDLKKRGTDRDLKCILLGVSRDLTNKVNDLKSAASDAALARALGDTDLLLR
                     DNIDVIVTPDTVDSGLDCTVEFGKDA"
     gene            complement(1422..1595)
                     /locus_tag="VF402_11890"
     CDS             complement(1422..1595)
                     /locus_tag="VF402_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801046.1"
                     /translation="MRKHSHARETFSHLMVALIIVIAGAAAIAWALNSFGTGCAAGVP
                     CHTDDGPHQVSYR"
BASE COUNT          336 a          606 c          599 g          374 t
ORIGIN      
        1 cgcctccctc tgccggcgtg ggcgcggccg gtcgcgctgg cggcgggggt cgtcctcggc
       61 ctgttcgccg ccggactgcc gcgcgcgccg ctgacgcccg ttacgtccgc cgctccggcc
      121 gttgcgggcc tgcgccagcc cttcgacgtc gacgcgctgg cgcaactgcg cgcgcaggga
      181 cggcccgtgc tggtcgacct gaccgccgcc tggtgcgtga cgtgcaaggt gaacgagcgg
      241 ctggccctgt ccaccgcgaa gttcgaaaaa gcgctgaaag cgaccggcac ggtctatatg
      301 gtcggcgact ggacgcatca ggacgcggcg atcacgcgct atctgagcct ttacggacgc
      361 tccggcgtgc cgctctacgt ctattacggc gcgcacgacg ccgcgccgcg gattctgccg
      421 cagatcctga acgcggacga ggtggtcagg gtgctggagg cgggagccag gtaagggtta
      481 tttgccacga aaagcacgaa gggacacgaa aagtcagaga cgctgagaga tcgggtttat
      541 tctctgtcgt cagtcgggat aatgcgcttc gcgcggcgga gcgatgcaat cttcgcttat
      601 gggtgacgat ttacgtcgtt gttcttgcgt agttttacgc gcatctaagt ccaaaaagcg
      661 cgctacactt tttaagatgc gctctaagtg cggcttcgcc gctctcatta tttctcgcta
      721 caactcgaaa agcgctgacc ttttcgtgtc ctttcgcgcc tttcgtggca aaaaccttca
      781 ggcgtcctta ccgaactcca ccgtgcaatc gagaccggaa tcgaccgtgt ccggcgtgac
      841 gatcacatcg atattgtcgc gcaataacag atcggtatcg cccaatgccc gcgccagggc
      901 ggcgtcgctg gcggcgcttt tcagatcgtt gaccttgttg gtcaggtcgc gcgacacgcc
      961 cagcaggata catttcaggt cgcggtcggt cccgcgtttt ttcaggtcga aatgcccctt
     1021 gaggtcgccc gccgacaggt tttgcaggcg ttgcagatag gtctgaaagc gcggcgcatc
     1081 gcgcagggcc agacttgggg ccttgctgaa atcctgcgtt tcggcgcgca gggcgttggc
     1141 gtcctgcacc agaccggcgt agagcggttc atgcgaaatc ggatcggtgg cgggccggtt
     1201 cgccgaatct tcggcaggct ggccttcgac aggctggcct tcggcgacgc gcacgatcgc
     1261 cgccggacac gcgggcgcga ccagatcagc cggcgccgcg ccgcgcaaca tcagggcgag
     1321 ggcgatcgaa cagtccatga cggcgctcct tcatcacaac gaagcgcaat ctgccggatt
     1381 tcacgcgcca atgggttaac gcgccgcttt ttgccggcgc tttaccgata gctgacctga
     1441 tgcggtccgt cgtcggtgtg gcagggcacg cccgcggcgc agccggtgcc gaagctgttg
     1501 agcgcccacg caatggcggc ggcgcccgcg atgacgatga tcaaggccac cataaggtgc
     1561 gagaaagtct ccctggcgtg agagtgttta cgcatgtcat gtcctcctta tcggaagtcg
     1621 caagataaca ccgttccgcc acatttaaca gactgttcat caaaaatttt gacgagagcg
     1681 tcagaaatgt cttgcgcctt ttctgtgcgg gccgcagcca agccgcccct tgtaaggcgg
     1741 cgcgccttgg tttaatcgcc gcttatgacc gacagcgcct ccacctcccg ccccgaatct
     1801 gatcgtcccg aatccgatcg tcccgaatct catcgccccg aagccagggc gccgcgcggt
     1861 ttcgccgaca agcgcgcgac tcagatcgcg atcgaacgcc gcctgatcga gacgg
//
LOCUS       DASVLL010000726         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_106680,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000726 DASVLL010000000
VERSION     DASVLL010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106680"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VF402_11895"
     CDS             complement(<1..643)
                     /locus_tag="VF402_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX7801047.1"
                     /translation="MKHARIIALFAAGLTLGLTLGLGGCVNVLPKVKPVQLYRFGYHP
                     GLLKDKDAAAVAPAAAPVPLMLGAISFPPASAGDQVMTTEDNEVSYVAQARWAASAQS
                     MFTEAVSEGFARSDQTVMLVPRGPTAADYRLDVSVRRFEASYSHGRPTVYIGVDARLL
                     RLSDRSVLAQRYISASVSVRRNDMTLISQGFDQATTEVVAGLTGFADASLKKAA"
     gene            complement(640..1575)
                     /locus_tag="VF402_11900"
     CDS             complement(640..1575)
                     /locus_tag="VF402_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7801048.1"
                     /translation="MERQAHYAAVGLVTLVLAAILLGFAFWFIKFDFNKDYGVYDVEF
                     NQPVDWLNKGAEVHFNGIKVGEVTSLRLGKPGTHQVFARVQLDAGTPVRTDSVATLMP
                     QGITGLLYMQISPGTVKAPLLRRKLGEAPPVIHSVESPLQKLLSGSGSVVEKTYESLD
                     RVNRLLSDQNIQSFTNTMNNIQAITADLKQRQQLLDDAHEAIVSAGQAADAVTQLAHS
                     TNGVMSEKLPATMDQIDAATQKLADAADNVSKLTQEIQKPVDQVNNSTLPALEESLQN
                     LNEAASSVKSLVDTVQSSPRGLIARPEAKERKVSQ"
     gene            complement(1585..2367)
                     /locus_tag="VF402_11905"
     CDS             complement(1585..2367)
                     /locus_tag="VF402_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7801049.1"
                     /translation="MSVAEHETDKAADAIEISGLTNRFGAHVVHDDLNLVVRRGEVLG
                     VVGGSGAGKTVLLNSILGLQRPAAGKIRIFGKNLAEAGEAEALAIRAKTGVLFQGGAL
                     FSTLSVAENIMAPILEFTRLSPKEAREIALLKLALVGLPLRAADLAPSELSGGMVKRT
                     ALARALALDAELLFLDEPTAGLDPIGAAAFDELIKDLADSLDLTVFMITHDLDSLYTV
                     CDEIAVLADKHVAAKDTVETLEKSDHPWIHEYFHGPRSRLKG"
     gene            complement(2367..>3125)
                     /locus_tag="VF402_11910"
     CDS             complement(2367..>3125)
                     /locus_tag="VF402_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7801050.1"
                     /translation="RFARGFMAQTAFLGHVTTVALNSLMRPARIRWPALINIMQRAGI
                     DSIPIVALTSLFIGAVIGFIGVLQLQQYGGGIFAIDMVAVFSLREFAPLITAILLAGR
                     SASAFAAEVGSMKMNQEIDAMRVMGIEPYEALVLPRILGLVIMSPFITMIAALAAMFG
                     GMLAIWSSLGAGPATFLQRLHDYVPFVDFFVGIVRPPFFAATIAIVGCWQGLNVKDDV
                     LSLGRQVTTAVVQSIFLVFMLDAMFAILFNGFDF"
BASE COUNT          518 a         1031 c         1046 g          530 t
ORIGIN      
        1 gcgcggcttt tttcaggctg gcgtcggcga agccggtcag cccggccacc acctcggtgg
       61 tcgcctgatc gaagccctgc gagatcagcg tcatgtcgtt ccggcgcacc gagacgctgg
      121 ccgagatgta gcgctgcgcc agcaccgaac ggtcggacag ccgcagcagg cgcgcgtcga
      181 cgccgatata gacggtcgga cggccgtggc tgtagctggc ctcgaagcgg cgcacgctga
      241 cgtccagccg gtagtccgcc gccgtcggcc cgcgcggcac cagcatcacc gtctggtccg
      301 agcgcgcgaa gccttccgac accgcctccg tgaacatgga ctgcgccgac gccgcccagc
      361 gcgcctgcgc gacgtaggag acttcgttgt cctcggtggt catcacctga tcgcccgcgc
      421 tggcgggcgg gaagctgatc gcgcccagca tcagcggaac cggcgccgcc gcgggcgcga
      481 ccgccgccgc gtccttgtcc ttgagcaggc cgggatggta gccgaagcga tagagctgca
      541 ccggcttgac cttgggcagg acgttgacgc agccgcccag gcccagggtc aggcccaggg
      601 tcagccccgc cgcgaaaagg gcgatgatgc gcgcgtgttt cattggctga cctttctttc
      661 cttggcttcc ggacgggcga tcaggccgcg cgggctggat tgcacggtgt cgaccaggct
      721 cttgaccgaa ctggcggcct cgttgaggtt ttgcaggctt tcctccagcg cgggcaaggt
      781 gctgttgttg acctgatcga ccggcttctg gatctcctgc gtcagcttgc tgacattgtc
      841 cgccgcgtcg gccagcttct gcgtcgccgc gtcgatctgg tccatcgtgg cgggcagttt
      901 ctcgctcatc acgccgttgg tcgaatgcgc cagttgcgtg accgcgtcgg cggcctgacc
      961 ggcgctgacg atggcctcgt gcgcgtcgtc cagcagttgc tggcgctgct tgaggtcggc
     1021 ggtgatcgcc tggatgttgt tcatggtgtt ggtgaaggac tggatgttct ggtcgctgag
     1081 cagccggttg acgcggtcga ggctctcata ggtcttctcc acgaccgagc cggagccgga
     1141 caacagcttt tgcagcgggc tttcgaccga atggatcacc ggcggcgcct cgcccagctt
     1201 gcggcgcagc aggggcgcct tcaccgtgcc cggcgaaatc tgcatgtaga gcagcccggt
     1261 gatgccctgc ggcatcaggg tggcgaccga gtcggtgcgc accggcgtgc ccgcgtcgag
     1321 ctgcacgcgc gcgaacacct gatgcgtgcc gggcttgccc agccgcagcg aggtgacctc
     1381 gccgaccttg atgccgttga aatgcacctc ggcgcccttg ttgagccagt cgaccggctg
     1441 gttgaactcg acgtcgtaaa cgccgtagtc cttgttgaag tcgaacttga tgaaccagaa
     1501 ggcgaacccc agcagaatgg ccgccagcac gagggtgacc agtccgaccg cggcgtaatg
     1561 ggcctgacgt tccataatct atccctaccc tttcagacgg cttctggggc cgtggaaata
     1621 ttcgtggatc catggatgat ccgacttttc gagagtctcg accgtgtcct tggcggcgac
     1681 atgcttgtcg gccagcaccg cgatctcgtc gcagaccgta tagaggctgt cgagatcgtg
     1741 ggtgatcatg aacacggtca ggtcgaggct gtcggccaga tccttgatca gttcgtcgaa
     1801 cgccgccgcc ccgatcgggt ccagccccgc cgtcggctcg tccagaaaca gaagctcggc
     1861 gtccagcgcc agcgcgcgcg ccagcgccgt gcgcttgacc atgccgcccg acagctcgga
     1921 cggcgccagg tccgccgcgc gcagcggcag cccgaccaga gccagtttca aaagcgcgat
     1981 ctcgcgcgcc tccttcgggc tgagccgggt gaattccagg atcggcgcca tgatgttttc
     2041 ggccacgctc agggtcgaga acagcgcccc gccctgaaac agcacgccgg tcttggcgcg
     2101 gatggccagc gcctcggcct cgcccgcctc ggcgaggttc ttgccgaaga tgcggatctt
     2161 gcccgccgcc ggtctttgca ggcccagaat agagttgagc agcaccgtct tgcccgcgcc
     2221 cgagccgccg accacgccca gcacctcgcc gcgcctgacc accagattca ggtcgtcatg
     2281 cacgacatgc gcgccgaagc ggttggtcag gccgctgatc tcgatggcgt ccgccgcctt
     2341 gtcggtttcg tgttccgcga cgctcatcag aagtcgaacc cgttgaacag gatggcgaac
     2401 atggcgtcca gcatgaagac gaggaagatc gactgcacca ccgcggtcgt gacctgacgg
     2461 cccaggctca gcacgtcgtc cttgacgttc agcccctgcc agcagccgac gatggcgatg
     2521 gtggcggcga agaagggcgg gcgcacgatg ccgacgaaga agtcgacgaa cggcacgtag
     2581 tcgtgcagcc gttgcaggaa ggtggccgga ccggcgccca ggctggacca gatggccagc
     2641 atgccgccga acatcgcggc cagcgccgcg atcatggtga tgaagggcga catgatgacg
     2701 aggccgagga tgcgcggcag caccagcgcc tcatacggct cgatgcccat gacgcgcatg
     2761 gcgtcgatct cctgattcat cttcatcgag ccgacctcgg cggcgaaggc cgaagccgaa
     2821 cgcccggcca gcaggatcgc cgtgatcagc ggcgcgaatt cgcgcaggga gaacaccgcc
     2881 accatgtcga tggcgaagat gccgccgccg tattgctgaa gctgcaacac gccgatgaag
     2941 ccgatcacgg cgccgatgaa caggctggtc agggccacga tcgggatcga gtcgatgccc
     3001 gcgcgctgca tgatgttgat cagggcgggc cagcggatgc gcgccgggcg catcaggctg
     3061 ttcagggcga ccgtcgtgac gtggcccagg aacgccgtct gcgccatgaa gccgcgcgcg
     3121 aaccg
//
LOCUS       DASVLL010000727         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_213155,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000727 DASVLL010000000
VERSION     DASVLL010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213155"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VF402_11915"
     CDS             complement(<1..1044)
                     /locus_tag="VF402_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX7801051.1"
                     /translation="MRRFLSQFVADRRAAVAILMALLMSIILLIAACAVDLGLVWLKS
                     RTLQGTADLAALAAAGDLGHAQAAAAAVAAANGGTVAEATTGVYDPMAGAGPATRFSA
                     ADADESNAAHVVLHDRAELVFGRMFYDRPIVLSRSATAASAKMAAFSLGSRLASLNGG
                     IANALLSGLTGSRISLSVMDYNALAGADIDLLSYVQALHAHAGLTAASFSQTLNSQIS
                     VGDALNVLIGQSPSAVKAPLSLVANSAARDTKIRLGDLIDTGVYGDQDHIVGGADSAI
                     RVSDMAMVNALLLLANQDRQVSLDLGAAVPGLAGVKVWLAVGERPNHSPWLTVTRTGQ
                     PVIRTAQARLYIEA"
     gene            complement(1044..1463)
                     /locus_tag="VF402_11920"
     CDS             complement(1044..1463)
                     /locus_tag="VF402_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7801052.1"
                     /translation="MRALRFSRWRRDARGATALEFALVAPILIMMLGGIIDYGNYFAT
                     AHAVQQTVNDAARAAIGGVTAAERLSLAQTTAAADLADYHFLTPGAGALTIDEGDERL
                     TLSLTYTPDGGGFELMPMAPGLPATVTRTAVIIRGGY"
     gene            complement(1575..1650)
                     /locus_tag="VF402_11925"
     tRNA            complement(1575..1650)
                     /locus_tag="VF402_11925"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1615..1617),aa:Ile,seq:cat)
BASE COUNT          315 a          697 c          622 g          291 t
ORIGIN      
        1 cgcctcgata tagagccgcg cctgcgcggt gcggatgacc ggctggccgg tgcgcgtcac
       61 ggtcagccac ggcgaatggt tcgggcgctc gcccacggcc agccagacct tgacgcccgc
      121 cagccccgga accgcggcgc cgaggtcgag gctgacctgc cggtcctgat tggccagcag
      181 cagcagcgcg ttcaccatcg ccatgtcgct gacgcggatg gcggaatcgg cgccgccgac
      241 gatatggtcc tgatcgccat agacgcccgt atcgatcagg tcgccgagcc ggattttcgt
      301 atcgcgcgcg gcgctgttcg ccaccaggct cagcggcgcc ttcacggcgg acgggctttg
      361 gccgatcagc acgttcagcg cgtcgcccac gctgatctgg ctgttcagcg tctggctgaa
      421 actggccgcc gtcaggcccg catgggcgtg cagcgcctgc acataggaca gaaggtcgat
      481 atcggcgccg gccagcgcgt tgtaatccat cacgctcagg ctgatccggc tgccggtcag
      541 gccgctgagc agcgcgttgg cgatcccgcc gttcagcgag gccagccgcg agccgaggct
      601 gaaggccgcc atcttcgccg acgcggccgt cgccgaacgc gacagcacga tgggccggtc
      661 gtagaacatc cgcccgaaga ccagttcggc ccggtcatgc agcacgacat gcgcggcgtt
      721 gctttcgtcc gcgtcggcgg cggaaaagcg cgtcgccggg cctgcgcccg ccatcggatc
      781 gtaaaccccg gtcgtcgcct cggccaccgt cccgccgttc gcggcggcga ccgctgccgc
      841 cgccgcctgc gcatggccga gatcgcccgc cgccgccagc gccgccagat ccgccgtccc
      901 ctgcaaggtg cggcttttca gccagaccag ccccagatcg accgcgcagg ccgcgatcag
      961 caggatgatg ctcatcagca gcgccatcag gatcgccacc gccgcccggc ggtctgcgac
     1021 gaattgtgag aggaagcgcc gcatcagtag cctccccgga tgatgacggc ggtgcgcgtc
     1081 accgtcgcgg gcagccccgg cgccatcggc atcagctcga agccgccgcc atcgggcgtg
     1141 taggtcaggc tcaacgtcag gcgctcatcc ccctcgtcga tcgtcagcgc gcccgcgccc
     1201 ggcgtcagga aatggtagtc ggccaggtcg gcggccgccg tcgtctgcgc cagcgacagc
     1261 cgctccgccg ccgtcacccc gccgatggcg gcgcgcgccg cgtcgttgac cgtctgctgc
     1321 acggcgtggg cggtggcgaa gtagttgccg taatcgatga tgccgccaag catcatgatc
     1381 aggatcggcg cgaccagcgc gaactccagc gccgtcgccc cgcgcgcgtc gcgccgccag
     1441 cgtgaaaacc tgagagcccg catgatgcaa ctccatagac cgaccgcgaa atcaatctat
     1501 agttgcatat accggtttac aatatcataa catgtattac catgccgcac gcgcagggat
     1561 tgtacaggag agattggtag gcgaggaggg actcgaaccc ccaaccagac cgttatgagc
     1621 ggtcggctct aaccattgag ctactcgccc cccgtaagag gaaacggcgt gctatcatga
     1681 cttgccgcgc gccgcaatcc gcatcggcgg cgccgcgcgc ttgtccacag cgccgttcag
     1741 gtcgggttca tgcgcgcccg gccaagatga tgtcaccggc gcgacatttc gcgcgcccca
     1801 gaggaaaaag gatcgcctat gtcgagatcg cttgtgaaac ccgccctgct ggccctggtc
     1861 ctgaccgccg ccgccgcgcc cgccgccttc gcgcaggttg cgtcggcgac cgcgcccgcg
     1921 ccgac
//
LOCUS       DASVLL010000728         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_374578,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000728 DASVLL010000000
VERSION     DASVLL010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374578"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF402_11930"
     CDS             complement(<1..209)
                     /locus_tag="VF402_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HEX7801053.1"
                     /translation="MSDGKDDKTNPPHGNDGSRTERAPLTLKPRAAGNVASGTVKQSF
                     SHGRSKTVVVETKRRIGAPPAGASP"
     gene            complement(388..996)
                     /locus_tag="VF402_11935"
     CDS             complement(388..996)
                     /locus_tag="VF402_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7801054.1"
                     /translation="MRRDIASGRSVDADGLIRFVVAPGGVLTPDLAHKLPGRGLWVAS
                     DRASLEIAIKKNIFSRAAKRQVKAGPDLIDLVHDLLRRRCLDLLGLARREGLVVNGFE
                     KVLAAIKSGRCAWLIEASDGAEDGRKRLLAAARAQSPAPPVCGGFDNDALSLALGQEN
                     AVHAALLPGRRVQRWTAEMRRLAGFEPLAPAAWTAASREDGP"
     gene            complement(1103..2743)
                     /gene="nusA"
                     /locus_tag="VF402_11940"
     CDS             complement(1103..2743)
                     /gene="nusA"
                     /locus_tag="VF402_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7801055.1"
                     /translation="MSFTGISANRQELLQIAEAVAREKAIDKSVVIASIEEAIQKAAR
                     SRYGADHDIRVHIDPRSGEMTITRVTTVVEDELLENEYAQKTLTDALRDDKEAFVGKE
                     YVEVLPPFELGRVQTQMARQVVTHKVREAEREHQYDEYKDRVGDIVNGTVRRVEYGNV
                     IVDLGKGEGIMRRDQSIPREAFQVNDRIRAYIYDVRRETKGPQIMLSRAHGGFMAKLF
                     AQEVPEIYDGVIEIKSVARDPGSRAKMAVLSNDNSIDPVGACVGMRGSRVQAVVAELQ
                     GEKIDIIQWSPDEATFIVNALAPAEVSKVVLDEEEDRVEVVVPDEQLSLAIGRRGQNV
                     RLASQLTGWQIDIITETQDSERRQKEFAERTVLFQEALDVDEVIAQLLVTEGFSTVED
                     LAYVDETEIASIEGFDEDTAQELQARARDFLEREAAEQDAKRRELGVEDEVLNVPGVT
                     LAMAVALGEAGVKTLEDLADLATDEIRGGFEQKGGERVRVPGALESFNLSVPDAENLI
                     LNARVAAGWIEAPVEDEADETVYDEEGETNDDVAELEQ"
BASE COUNT          402 a          951 c          870 g          528 t
ORIGIN      
        1 tgcgggctgg cgccggcggg cggcgcgccg atgcggcgct tggtctccac caccacggtc
       61 ttggagcggc cgtggctgaa gctttgcttg accgtgccgc tggccacatt gccggcggcg
      121 cgcggcttca gcgtcagcgg cgcgcgttcg gtccgggaac cgtcgtttcc gtgcgggggg
      181 ttggtcttat cgtcttttcc gtcgctcatc cggctttata actatctctt tttgaggcgg
      241 cgatgcgccg gcctcgtctt caaaaattgt cgccgccgcc ggaaaacctg cgcggaagcg
      301 tccgaaactc gtaaatccgc ttaatcgcct gcggataaat caccctgacc gtttggcgtc
      361 aaggtgtgcg gcgcttttaa atccgtgtca cggcccatcc tcccgtgaag ccgccgtcca
      421 cgcggcgggc gccagcggct cgaaacccgc cagccgccgc atctccgccg tccagcgctg
      481 tacccgccgt ccgggcagaa gcgccgcatg cacggcgttt tcctgcccca gcgccaggct
      541 cagcgcgtca ttgtcgaagc cgccgcagac cgggggcgcg gggctttgcg cgcgcgccgc
      601 cgccagaagc cgcttgcgcc cgtcctcggc gccgtcggac gcctcgatca gccacgcgca
      661 tctgccgctt ttgatcgcgg ccagcacctt ctcgaaaccg ttgacgacaa ggccctcgcg
      721 ccgcgccaga cccagaaggt cgaggcagcg ccgccgcaag agatcatgca cgagatcgat
      781 gaggtccggc cccgccttga cctgacgttt ggccgcacgg ctgaatatgt tctttttgat
      841 cgcgatttca aggctggcgc ggtcggaagc gacccacagg ccgcgtccgg gcagcttgtg
      901 cgcgaggtcc ggcgtcagaa cgccgccggg cgcgacgaca aagcggatca ggccgtccgc
      961 gtccacggaa cggcctgagg cgatgtcgcg gcgcatcagg atgcggccca aaatacgacc
     1021 acgcgaatct tcgcacagca cctcgacgcc cgtcgcttcg tcagcgacgg ggccggggtc
     1081 gataaggttc agatgtgcgg gctcattgtt cgagttcggc gacgtcgtca ttcgtctcgc
     1141 cctcctcgtc ataaaccgtt tcgtcggcct cgtcctcaac cggggcctcg atccagcccg
     1201 ccgcgacgcg cgcgttcagg atcaggtttt cggcgtcggg gacggacagg ttgaacgatt
     1261 ccagcgcgcc gggcacgcgc acgcgctcgc cgcccttctg ctcgaagccg ccgcggattt
     1321 cgtcggtggc gagatcggcc agatcctcca gcgtcttgac gcccgcctcg cccagcgcca
     1381 ccgccatggc cagcgtcacg cccggcacgt tcagcacctc gtcctcgacg cccagttcgc
     1441 ggcgtttcgc gtcctgctcg gcggcctcgc gctccaggaa gtcgcgggcg cgcgcctgca
     1501 attcctgcgc ggtgtcctcg tcgaagccct cgatgctggc gatttccgtc tcatcgacat
     1561 aggccagatc ctcgaccgtc gagaagcctt cggtgaccag cagttgcgcg atgacctcgt
     1621 cgacgtcgag cgcttcctgg aacaggacgg tgcgctcggc gaactccttc tgacggcgct
     1681 ccgaatcctg cgtttcggtg atgatgtcga tctgccagcc ggtcagttgc gaggccagac
     1741 gcacattctg gccgcgacgg ccgatggcca gcgacaattg ctcgtccggc accacgacct
     1801 cgacgcggtc ctcttcctcg tccagcacca ccttggagac ttccgccggg gccagcgcgt
     1861 tgacgatgaa ggtcgcctcg tccggcgacc actggatgat gtcgatcttc tcgccctgca
     1921 attcggccac caccgcctgc acgcgcgagc cgcgcatgcc gacgcaggcg ccgaccgggt
     1981 cgatggagtt gtcgttgctg agcacggcca tcttggcgcg cgaaccgggg tcgcgggcga
     2041 ccgatttgat ctcgatcacg ccgtcgtaga tttccggcac ttcctgcgcg aacagcttgg
     2101 ccatgaagcc gccgtgggcg cgcgacagca tgatctgcgg ccccttggtc tcgcggcgca
     2161 cgtcgtagat ataggcgcgg atgcggtcat tgacctgaaa cgcctcgcgc gggatcgact
     2221 ggtcgcggcg catgatgcct tcgcccttgc cgagatcgac gatgacgttg ccgtattcga
     2281 cgcggcgcac cgtgccgttg acgatgtcgc cgacgcggtc cttgtattcg tcgtactgat
     2341 gctcgcgctc ggcctcgcgc accttgtgcg tcacgacctg acgcgccatc tgggtctgga
     2401 cgcggcccag ctcgaacggc ggcagcacct cgacatattc cttgccgacg aacgcctcct
     2461 tatcgtcgcg cagcgcgtcg gtcagcgtct tttgcgcgta ttcgttctcc agcagttcgt
     2521 cctcgaccac cgtggtgacg cgggtgatgg tcatttcgcc gctgcgcggg tcgatgtgga
     2581 cgcgaatgtc gtgatcggcg ccgtagcgcg agcgcgccgc cttctggatc gcttcctcga
     2641 tggaggcgat gaccacggac ttgtcgatcg ccttctcgcg ggcgacagct tcggcgattt
     2701 gcagcaactc ctggcggttg gcgctgattc cggtaaagct catggcttaa t
//
LOCUS       DASVLL010000729         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_106761,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000729 DASVLL010000000
VERSION     DASVLL010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106761"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VF402_11945"
     CDS             <1..520
                     /locus_tag="VF402_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7801056.1"
                     /translation="DGLGGLDILVNNAGLIRRQDAVDFSEKDWDDVMNVNIKSAFFMS
                     QAAGRHFIAQGSGKIINIASMLSFQGGIRVPSYTASKSGIAGITRLLANEWANKGVNI
                     NAIAPGYMATDNTAQIRADEQREKAILDRIPAGRWGTPADLAGTAVFLAAQASDYVNG
                     AVIPVDGGWLAR"
     gene            complement(592..975)
                     /gene="cyoD"
                     /locus_tag="VF402_11950"
     CDS             complement(592..975)
                     /gene="cyoD"
                     /locus_tag="VF402_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEX7801057.1"
                     /translation="MSDNHHDNQHGHDHGHDESGASHGSVGSYLFGFIASLVLTAIPF
                     ALVMTHAMPAHTLIPVIVVIGVVQIIVHLIYFLHMNTSSTQSWNNLAFFFTLVILGIL
                     IIGTLWVMYHMNANMMPGMMPTNIG"
     gene            complement(975..1598)
                     /gene="cyoC"
                     /locus_tag="VF402_11955"
     CDS             complement(975..1598)
                     /gene="cyoC"
                     /locus_tag="VF402_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEX7801058.1"
                     /translation="MSHPASLGESLTAQVDHHHEHNDEAPKVTLGFWIYLMTDCILFA
                     SVFATFAVLRNATAGGPSGREIFELPYVAVETGLLLVSSLTYGLAMIAMEAGKLKSVF
                     TWLILTGLCGLGFLGMEVNEFAKLIHEGAGPDRSAFLSAFFTLVGTHGLHVTCGMIWL
                     VTMLVHLKRRGLSAANRVRLMELSLFWHFLDIIWIGVFTIVYLMGVA"
     gene            complement(1602..3596)
                     /gene="cyoB"
                     /locus_tag="VF402_11960"
     CDS             complement(1602..3596)
                     /gene="cyoB"
                     /locus_tag="VF402_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEX7801059.1"
                     /translation="MDLQHFIFGKLNIDSVPWHEPIIMVAGGGMVGAAVLVLGLTTYF
                     KAWPYIWKEWLTSVDHKRIGVMYIILALIMLLRGFADALMMRGQQALASAGGHGYLPP
                     SHFDQIFSAHGVIMIFFMAMPFIIGLMNIVMPLQIGARDVAFPYLNSMSFWLTASGAV
                     LVNISLVIGEFAHTGWLAYPPLSELAYSPGVGQDYYIWALQISGLGTLLSGVNLITTI
                     FKMRAPGMRLMDMPIFTWTTLCANALICAAFPILTVTLALLGADRYIGTHFFTNDGGG
                     NAMMYINLIWAWGHPEVYILVLPCFGIYSEVVATFSSKPLFGYKSMVYATVCITILSF
                     MVWLHHFFTMGSGADVNAFFGIATMIISIPTGVKIFNWLFTLYRGRVRLEIPILWTLA
                     FIITFAIGGMTGVLLAVPGADFVLHNSVFLIAHFHNVIIGGVVFAVMAGMNYWFPKAF
                     GFKLHEGLGKVSVFCWVVGFYIAFMPLYALGLMGMTRRLDYMNNPVWHAYLIVAALGA
                     LIIFVGILSTIAQIVVSVIQRDKLKDVTGDPWGGRTLEWATSSPPPFYNFAELPPVTG
                     RDAFWAMKVKGEAHQAPAAYAPIHMPHNTPTGFLIGVVSAGLGFGLVWHIWWMAIGAL
                     LVMIGLGIAHSFNENRDYYVPSEQVAEIEGAHLQRLKVEA"
     gene            complement(3602..>3831)
                     /locus_tag="VF402_11965"
     CDS             complement(3602..>3831)
                     /locus_tag="VF402_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HEX7801060.1"
                     /translation="DGAAYKLLERPADKLPVAYYGSVAPNLYHDILNKCADGGACMDA
                     SMGSMAEGAQCKPGEQVATTGAAVKPQARRS"
BASE COUNT          794 a         1195 c         1223 g          619 t
ORIGIN      
        1 cgacggcctc ggcggcctcg acatcctcgt caacaacgcc gggctgatcc gccgtcagga
       61 cgcggtcgac ttctccgaaa aggactggga cgacgtgatg aacgtcaaca tcaagtccgc
      121 cttcttcatg tcgcaggccg cagggcgtca cttcatcgcg caaggttcgg gcaagatcat
      181 caacatcgcc tcgatgctct cgttccaggg cggcatccgc gtgccctcct acaccgcctc
      241 gaagtccggc atcgcaggca tcacccgcct gctggccaac gaatgggcga acaagggcgt
      301 caacatcaac gccatcgccc ccggctacat ggccaccgac aacaccgccc agatccgcgc
      361 cgacgaacag cgcgaaaagg ccatcctcga ccgcatcccc gccggacgat ggggaacccc
      421 cgccgacctc gccggaaccg ccgtcttcct cgccgcacag gcctccgatt acgtcaacgg
      481 agccgtcatc cccgtcgatg gtgggtggct ggcgaggtaa gccgccggac ccggacacga
      541 aaaaggcggc tccgacggag ccgccttttt tattgtgcag atgcagacgc ctcagccgat
      601 attggtcggc atcatgcccg gcatcatatt ggcgttcatg tggtacatga cccacaaggt
      661 gccaatgatc agaatgccca gaatgaccag cgtgaagaag aaggccaggt tgttccagct
      721 ctgcgtcgag gacgtgttca tgtgcaggaa gtagatcagg tgcacgatga tctgcacgac
      781 gccgatcacg acgatgaccg ggatcagcgt gtgcgcgggc atggcgtgcg tcatgacgag
      841 ggcgaacggg atcgccgtca gcacgagcga ggcgataaag ccgaacaggt aggagccgac
      901 gctgccgtgc gaggcgccgc tttcgtcatg cccgtgatca tgcccgtgtt ggttgtcgtg
      961 gtgattgtcg ctcattacgc aactcccatc agatagacga tcgtgaacac gccgatccag
     1021 atgatgtcga ggaagtgcca gaacaggctc agctccatca ggcggacgcg gttggcggcg
     1081 ctcaggccgc ggcgctttaa gtgaaccagc atggtcacca gccagatcat gccgcaggtg
     1141 acgtgcaggc cgtgcgtgcc gaccagcgtg aagaaggccg acaggaaggc cgagcggtcg
     1201 ggaccggcgc cttcgtggat cagcttggcg aattcgttga cttccatgcc gaggaagccg
     1261 aggccgcaca ggccggtcag gatcagccag gtgaacaccg acttcagctt gcccgcctcc
     1321 atcgcgatca tggccagccc gtaggtcagc gacgagacca gcagcaggcc ggtttcgacc
     1381 gccacatagg gcagctcgaa aatctccctg cccgacggcc cgcccgccgt ggcgttgcgc
     1441 agcaccgcga aggtggcgaa caccgaggcg aacaggatgc agtccgtcat caggtagatc
     1501 cagaagccca gggtcacctt gggggcttcg tcgttatgct catggtgatg atcgacctga
     1561 gcggtcaggc tttcgccgag gcttgcggga tggctcatgg atcaggcctc caccttgaga
     1621 cgttgcagat gcgcgccttc gatttcggcc acctgttcgg acggcacgta gtagtcgcgg
     1681 ttttcgttga agctgtgggc gatgcccagg ccgatcatga ccagcagcgc gccgatggcc
     1741 atccaccaga tatgccagac cagaccgaag cccagccccg ccgacaccac gccgatcagg
     1801 aagccggtcg gggtgttgtg cggcatgtgg atcggcgcgt aagccgccgg ggcctgatgc
     1861 gcctcgccct tgaccttcat cgcccagaag gcgtcgcggc ccgtgaccgg cggcagttcg
     1921 gcgaagttgt agaagggcgg cggcgacgag gtcgcccatt ccagcgtgcg tccgccccac
     1981 ggatcgccgg tgacgtcctt gagcttgtcg cgctggatga ccgagacgac gatctgcgcg
     2041 atggtggaca ggatgccgac gaagatgatc agcgcgccca gggccgccac gatcagatag
     2101 gcgtgccaca ccggattgtt catgtagtcg aggcggcgcg tcatgcccat caggcccagc
     2161 gcgtacagcg gcatgaaggc gatgtagaag ccgaccaccc agcagaacac cgacaccttg
     2221 cccagacctt cgtgcaactt gaagccgaac gccttgggga accagtagtt catgccggcc
     2281 atgaccgcga acaccacgcc gccgatgatg acgttatgga agtgggcgat caggaagacc
     2341 gagttgtgca gcacgaagtc cgcgcccggc accgccagca gaacgccggt catgccgccg
     2401 atggcgaagg tgatgatgaa ggccagcgtc cacaggatcg ggatttcgag gcgcacgcgc
     2461 ccgcgataga gggtgaacag ccagttgaag atcttaaccc cggtcgggat cgagatgatc
     2521 atggtcgcga tgccgaagaa ggcgttgacg tccgcgcccg aacccatggt gaagaagtgg
     2581 tgcagccaca ccatgaagga caggatggtg atgcacaccg tcgcatagac catcgacttg
     2641 tagccgaaca aaggcttgga cgagaaggtc gccacgactt cggaatagat gccgaagcac
     2701 ggcaggacca gaatgtacac ttcggggtgg ccccacgccc agatcaggtt gatgtacatc
     2761 atggcgttgc cgccgccgtc attggtgaag aagtgcgtgc cgatgtagcg gtccgcgccc
     2821 agaagggcca gcgtcacggt caggatcggg aaggccgcgc agatcagcgc gttggcgcac
     2881 agggtcgtcc aggtgaagat cggcatgtcc atcaggcgca tgccgggcgc gcgcatcttg
     2941 aagatggtgg tgatcaggtt caccccggac agaagcgtgc cgaggcccga aatctgcaag
     3001 gcccagatat agtagtcctg cccgacgccc ggcgaatagg ccagctccga caagggcgga
     3061 taggccagcc agccggtatg ggcgaattcg ccgatgacca gcgagatgtt gaccagcacc
     3121 gcgcccgagg ccgtcagcca gaagctcatc gagttcaggt acggaaaggc cacgtcgcgc
     3181 gcgccgatct gcaacggcat gacgatgttc atcaggccga tgatgaacgg catggccatg
     3241 aagaagatca tgatgacgcc gtgcgccgag aagatctggt cgaagtgcga cggcggcaga
     3301 tagccgtgcc cgcccgccga ggccagcgcc tgctggccgc gcatcatcag cgcgtcggcg
     3361 aagccgcgca gcagcatgat cagcgccagg atgatgtaca tcaccccgat gcgcttgtga
     3421 tcgaccgagg tcagccactc tttccagata tagggccacg ccttgaagta ggtggtcagg
     3481 cccaggacca gaaccgccgc gccgaccatg ccgccgccgg cgaccatgat gatcggctcg
     3541 tgccacggca ccgagtcgat gttcagcttg ccgaagatga aatgttgcag atccatgtga
     3601 cttaactccg gcgcgcttgc ggcttgacgg cggctccggt cgttgcgacc tgctcgcccg
     3661 gcttgcactg ggcgccttcc gccatcgacc ccatagaggc gtccatgcac gcgccgccgt
     3721 cggcgcactt gttgagaata tcgtgataaa ggttcggcgc caccgagccg taataggcga
     3781 ccggcagctt gtccgccgga cgttccagca gcttgtaggc cgcgccgtcc a
//
LOCUS       DASVLL010000730         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_106955,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000730 DASVLL010000000
VERSION     DASVLL010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106955"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VF402_11970"
     CDS             complement(<1..540)
                     /locus_tag="VF402_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7801061.1"
                     /translation="MKIPHTKLAAPAAALFFALTAPCHAAPSGFDIAVTVDDLPAHGS
                     LPKDMTRTQIADRVVAALKAHGAPQVYGFVNAVRLEQEPDSAAVLDIWRKAGFPLGNH
                     TYSHPNIRQVGYDAFIADLEKGEPVVAQHMQGPNLTENDWKWLRFPFLSAGDGETHQK
                     VMDYLHAHGYHIADVSMSFS"
     gene            789..1817
                     /gene="hppD"
                     /locus_tag="VF402_11975"
     CDS             789..1817
                     /gene="hppD"
                     /locus_tag="VF402_11975"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX7801062.1"
                     /translation="MPDPAPDANPLGLNGFAFVEFTGPDAGVMAAALEKLGFTAAAHH
                     PARDVVLYRQGEARFLLNHAATRQARDFAAQHGPSANGMGFVVADPEAALALAVRRGA
                     EPVADALFPHALQGIGSALLYLVAAEAFADWPALPGAGGGGVGFRRIDHLTHNVRRGR
                     MRHWSDFYRSIFGFSEQRYFDIKGQASGLFSQAMIAPDKTIRIPLNESQDDRSQIEEF
                     LTAYNGEGIQHIALTTDDIHATVEALRARGVALQDTPDAYYDLVDRRVPGHGEDLARM
                     RENRILIDGDVEKDGVLLQIFTENTFGPIFFEIIQRKGNDGFGNGNFQALFESIELDQ
                     IRRGVIKV"
     gene            2011..>2875
                     /locus_tag="VF402_11980"
     CDS             2011..>2875
                     /locus_tag="VF402_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX7801063.1"
                     /translation="MYMTGFGNHFATEAVPGALPQGQNSPQHTPFGLYAEQLSGTAFT
                     APRAHNLRSWLYRLRPSADHAPFTPVEDAAPLASPTPNRLRWSPPPLPEAAADFIDGL
                     FALAGNGDPARRTGVRACVYAANQSMARVFFNADAEMMIVPQHGTLDIFTEMGRMTVA
                     PLSIAVIPRGVRFRVAIDGEARGYCLENSGAPFRLPELGPIGANGLANARDFETPAAA
                     FEDREAPTELLQKYCGRLWRVMLPHSPLDVVAWHGNYAPYRYDLRRFNTINTVSFDHP
                     DPSIFTVLTSPS"
BASE COUNT          480 a          956 c          925 g          514 t
ORIGIN      
        1 ggaaaagctc atcgacacgt cggcgatgtg gtagccgtgc gcgtgcaggt aatccatcac
       61 cttctggtgc gtctcgccgt cgcccgcgct caggaacgga aagcgcagcc atttccagtc
      121 attttccgta agattcggtc cctgcatatg ctgcgcgaca accggctcgc ctttttccag
      181 gtcggcgatg aaggcgtcat agccgacctg gcggatattg gggtggctgt aggtatggtt
      241 gccgagcggg aagcccgcct tgcgccagat gtcgagcacg gcggcggagt ccggctcctg
      301 ttcgaggcgc accgcgttga cgaaaccata gacctgcggc gcgccgtgcg ccttgagcgc
      361 ggcgacgacc ctgtcggcga tctgggtccg ggtcatgtcc ttgggcaggc tgccgtgcgc
      421 gggcaggtca tcgacggtga cggcgatatc gaagcccgac ggcgccgcgt gacacggcgc
      481 cgtcagggcg aaaaagaggg cggcggccgg agccgccagc ttcgtatgcg gtatcttcat
      541 ccgtttattt tttctcgacc tgagcttgaa ccggcctctg cgtgggccgt taccgctaac
      601 atgcgccgga aaggcgagtc tggcaagctg tatttccgcc ggattgtgcg caaacgacgg
      661 gcgtgacatt tttgcccgta aaaccggcgg ccgctcacac aagcttgata aaaaacgcgc
      721 gcgaaaccgg ttgccaatga aattacatcg atatagtttc attggatact tttgccggaa
      781 tcctcatcat gcccgacccc gccccggacg ccaatccctt gggactcaac ggtttcgcct
      841 tcgtggagtt caccggcccc gacgccggcg tcatggcggc ggcgctggaa aagctcggct
      901 ttaccgccgc cgctcatcat ccggcgcgcg acgtggtcct gtaccgccag ggcgaggcgc
      961 gcttcctgct caaccacgcc gccacccggc aggcgcgcga ttttgctgcg cagcatggtc
     1021 cgtcggccaa cggcatgggc tttgtcgtcg ccgacccgga ggcggcgctg gccctggccg
     1081 tccggcgcgg ggcggaaccg gtcgcggacg ctttgtttcc ccatgcgctg caagggatcg
     1141 gtagcgcctt gctgtacctc gtcgcggcgg aggcgttcgc ggactggccc gccctgcccg
     1201 gcgcgggcgg cggcggggtc ggcttccggc gaatcgatca cctgacgcac aatgtgcggc
     1261 gcgggcggat gcggcattgg tcggacttct accgcagcat tttcggcttc agcgagcaga
     1321 gatatttcga catcaagggg caggccagcg gcctgttctc gcaggccatg atcgcgcccg
     1381 acaaaacgat ccgcatcccg ctcaacgaat cgcaggacga ccgcagccag atcgaggaat
     1441 tcctgaccgc ctataacggc gaaggcatcc agcacatcgc cctgaccacg gacgacatcc
     1501 acgccacggt cgaagccctg cgcgcgcgcg gcgtggcgtt gcaggacacg ccagacgcct
     1561 attacgatct ggtcgacagg cgcgtgccgg gccacggcga ggatctggcg cggatgcgcg
     1621 aaaaccgcat cctgatcgac ggcgatgtgg aaaaggacgg cgttctgttg cagattttca
     1681 ccgaaaacac cttcgggccg atcttcttcg agatcataca gcgcaagggc aatgacggct
     1741 tcggcaacgg caatttccag gcgctgttcg agtcgatcga actggaccag atccgccgcg
     1801 gcgtgatcaa ggtttgaatc ccctctcccc gcttgcgggg agaggacgag gagcaaggcg
     1861 aagccgccga tcctcgggtg aggggctgcc gacgactgag cgcaatcgtc gggcggcccc
     1921 tcacccgatc gccttcgcct gacggctcgg ctctcgtcct ctcccctcgt ggaggggaga
     1981 ggggaagata ggatataagg agaggccgcg atgtatatga ccggtttcgg caatcatttc
     2041 gccaccgagg cggttcccgg cgcgctgccg cagggacaga attcgccgca gcatacgccg
     2101 ttcggcctct atgccgagca actgtccggc acggccttca ccgcgccgcg cgcgcacaat
     2161 ctgcgttcgt ggctctaccg gttgcggccg tcggcggacc atgcgccgtt tacgcccgtc
     2221 gaggacgcgg cgccgctcgc atcgccgacg cccaaccggt tgcgctggtc gccgccgccc
     2281 ctgccggagg cggcggccga cttcatcgac ggcctgttcg cgctggccgg caatggcgat
     2341 ccggcgcggc ggacgggcgt gcgcgcctgc gtttacgccg ccaaccagag catggcgcgc
     2401 gtcttcttca acgccgacgc cgagatgatg atcgttccgc agcacgggac gctcgacatc
     2461 ttcaccgaga tgggccgcat gacggtcgcg cccttgtcca tcgcggtgat tccgcgtggc
     2521 gtgcgtttcc gcgtcgccat cgacggcgaa gcgcgcggct attgcctgga gaattccggc
     2581 gcgccgttcc gcctgcccga actcggcccc atcggcgcca acggcctggc caatgcgcgc
     2641 gatttcgaga cgcccgccgc cgccttcgag gatcgcgagg cgccgacgga attgctgcaa
     2701 aaatattgcg gccggttgtg gcgcgtcatg ctgccgcatt cgccgctcga cgtcgtcgca
     2761 tggcacggca attacgcgcc ctaccgctac gacctgcgcc gtttcaacac gatcaacacg
     2821 gtcagtttcg accatcccga cccgtcgatc ttcaccgtcc tgacctcgcc gtccg
//
LOCUS       DASVLL010000731         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_267706,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000731 DASVLL010000000
VERSION     DASVLL010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267706"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..553
                     /locus_tag="VF402_11985"
     CDS             113..553
                     /locus_tag="VF402_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801064.1"
                     /translation="MSDNDKLLRQAEGLAGFRRLAMIVNAVSYAAWLAGIAMAGAAEN
                     RGLWLAMQASGFIVWLVSLMAVLWTIRRARRRRDLGALIDDERTAMLTTRAFQVGYWV
                     LLAPLALLYTASYFVSLDLRSLLLALMALGVAAPSLTYALLYRN"
     gene            556..768
                     /locus_tag="VF402_11990"
     CDS             556..768
                     /locus_tag="VF402_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7801065.1"
                     /translation="MRLCNRLKDHRAARGWTQADLAVMAGVSRKTINTIENEVFTPSA
                     LLALKLAQVFAVAVEDLFYLEGGSAG"
     gene            complement(757..>1500)
                     /locus_tag="VF402_11995"
     CDS             complement(757..>1500)
                     /locus_tag="VF402_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7801066.1"
                     /translation="GARKNRSEDTGAAQARLAQRQRLEAESAAAGARARVEVLQPFAV
                     APVAAGLPAHKPVAQLDRVSAGYDGHPVLRDLTLHITGPERIAITGANGSGKSTLLSV
                     LAGRLQVLSGGASVCVPAALLDQHADLLRPEDSIRDNFRRLHPGLDENACRAALARFM
                     FRADAALQTVATLSGGQRLRAALACVLGGPKPPQLLLLDEPSNHLDLDALQAVEAGLN
                     AYDGALVVVSHDERFLDAIGVTRRIALSG"
BASE COUNT          214 a          478 c          533 g          275 t
ORIGIN      
        1 tctctctatc ccgaaccccc gaagcctcgg cttcgggggt tttgttttgc gcttgacggt
       61 gtgtcaccta tgggttactt gtaacctgtg ggtgatattc atggagggca tcatgtccga
      121 taacgacaag ctgctgcgtc aggccgaggg gctggccggt ttccggcgtc tggcgatgat
      181 cgtgaacgcc gtcagctatg cggcgtggct ggcgggcatc gcgatggcgg gcgcggcgga
      241 gaatcgcggc ctctggctgg ccatgcaggc gtccggtttc atcgtctggc tcgtcagcct
      301 gatggccgtt ttgtggacga tccggcgcgc gcggcggcgg cgcgatctgg gcgcgctgat
      361 cgacgacgag cgcacggcca tgctgacgac gcgcgccttt caggtcggct actgggttct
      421 gctggcgccg ttggcgctgc tttatacggc cagttatttc gtcagtctcg acctgcggtc
      481 cttgttgctg gcgcttatgg cgctgggcgt ggccgcgccg tcgctgacct acgccctgtt
      541 gtacaggaac tgacggtgag actttgcaac cgtttgaaag accatcgcgc ggcgcgcggc
      601 tggacgcagg ccgatctggc ggtcatggcg ggcgtgtcgc gcaagacgat caacaccatc
      661 gagaacgagg tgttcacgcc gtcggccctg ctggcgctga agctggcgca ggtcttcgcc
      721 gtcgccgtcg aagacctgtt ctatcttgaa ggcgggtcag ccggatagag cgatccggcg
      781 cgtgacgccg atggcgtcga gaaagcgctc atcatggctg accacgacca gcgcgccgtc
      841 ataggcgttc aggcccgcct ccaccgcttg cagcgcgtcg agatcgagat ggttgctcgg
      901 ctcgtccagc aacagaagct gcggcggctt cggcccgccc aggacgcagg ccagcgccgc
      961 gcgcaacctc tggccgccgc tcagcgtcgc cacggtttgc agcgccgcgt cggcgcggaa
     1021 catgaagcgc gccagcgccg cgcggcaggc gttttcgtcc agaccgggat gcaggcggcg
     1081 gaaattgtcg cggatcgaat cttcgggccg cagcaggtcg gcgtgctgat ccagcagggc
     1141 ggctggaacg cataccgatg cgccgccgga cagaacttgc aaccgtccgg ccagcacgct
     1201 cagcagggtg gacttgcccg acccgttcgc accggtgatc gcgatgcgct caggcccggt
     1261 gatgtgcagg gtgagatcgc gtaagaccgg gtggccgtca taaccggcgc tgacccgatc
     1321 cagttgcgcc accggtttat gcgcgggcag gccggcagcg acgggcgcga cggcgaaggg
     1381 ctgcaacacc tccacgcggg cgcgggcccc ggcggcggcg ctttcggctt cgagccgttg
     1441 gcgctgggcc agccgcgcct gcgccgcgcc ggtgtcctcg ctgcggtttt tgcgcgcgcc
//
LOCUS       DASVLL010000732         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_50230, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000732 DASVLL010000000
VERSION     DASVLL010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50230"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..618
                     /locus_tag="VF402_12000"
     CDS             46..618
                     /locus_tag="VF402_12000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEX7801067.1"
                     /translation="MTHRDDDPFIRRRTPESAAMQAEVRRAMMLTAALNRLGFDDAAE
                     IRARFGELTGRTPDDGFMLIPPFYATGGTRLRVGRNVFINQNCTFYDLGGIEIGDDVM
                     IGPNVSLITSGHPLSPARRRDGVTARPIVIGKNVWIAAGVTVIGGVTIGDGAVIAAGA
                     VVTRDVSPNTLAGGNPARAIRRLDDGDPRP"
     gene            682..921
                     /locus_tag="VF402_12005"
     CDS             682..921
                     /locus_tag="VF402_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7801068.1"
                     /translation="MELKITRIGNSLGVILPRELLIRLGLDKGDSLFVSETPEGFAAS
                     PYDPAFAAQMEAARNLMKSRRNALHELGKPEPEAG"
     gene            921..1319
                     /locus_tag="VF402_12010"
     CDS             921..1319
                     /locus_tag="VF402_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="death-on-curing protein"
                     /protein_id="HEX7801069.1"
                     /translation="MALALIADGVIEAAQAQLAAAHGGRAGLRERSMLRLLYARARML
                     SESASTDAAALAACYGYGLARDRLFYSQNTGVALLSVELALRLNGCRLTADDTACFLS
                     LSLAGQGEPGAESFAGWIRGHIAEQARDAA"
     gene            1465..>1977
                     /locus_tag="VF402_12015"
     CDS             1465..>1977
                     /locus_tag="VF402_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7801070.1"
                     /translation="MPDTSPDSPSPDASDRKPTIIDVAERAGVSIKTVSRVINNERHV
                     AAATRLRVREAVEALGFEANASARGMRRRAADRSWVIAQLYGDPGGVYTANIQFGLLE
                     RCQYFGYHLAVEELDYQNPNVERRARDLIPRLRPDGAVLTAPLTDDAIILDVLDEAGV
                     PYVRVTPLKED"
BASE COUNT          320 a          709 c          662 g          286 t
ORIGIN      
        1 tggcctttgc ggctatagtc gccgaagcca tcgaggagaa ccgccatgac gcaccgagac
       61 gacgatccgt tcatccgccg ccgcacgccg gaatcggcgg ccatgcaggc ggaggtgcgg
      121 cgcgccatga tgctgaccgc cgcgctcaac cggctgggct tcgatgacgc cgccgagatc
      181 cgcgcccgct tcggcgagct gaccgggcgg acgccggacg acggcttcat gctgatcccg
      241 cccttctacg ccacgggcgg gacgcgcctc cgcgtcgggc gcaacgtctt catcaaccag
      301 aactgcacct tctacgacct cggcgggatc gagatcggcg acgacgtgat gatcgggccg
      361 aacgtcagcc tgatcacctc cggccacccg ctttcgccgg cgcggcggcg cgacggcgtc
      421 accgcccggc cgatcgtcat cggcaaaaac gtctggatcg cggcgggcgt gaccgtgatc
      481 ggcggcgtga cgatcggcga cggcgcggtc atcgcggcgg gcgcggtcgt caccagggac
      541 gtgtcgccga acacgctggc cggcggcaat ccggcgcgcg cgatccgcag gctggacgac
      601 ggcgaccctc gcccgtaacc ttgcgtacat acatccgtac tacactgtcc ggcagagagc
      661 cgcctgcaac aggacaccgt tatggagctg aagatcacgc gcatcggcaa ttcgctgggc
      721 gtcattctgc cgcgcgagct gctgatccgg ctgggcctcg acaagggcga cagcctgttc
      781 gtgtccgaga cgcccgaagg cttcgccgcc tcgccctacg atccggcctt cgcggcgcag
      841 atggaggcgg cgcgcaacct gatgaaatcg cgccgcaacg cgctgcacga actgggcaag
      901 cccgaaccgg aggccggttg atggcgctgg ccctgatcgc cgacggcgtg atcgaggccg
      961 cgcaggcgca actggccgcc gcgcacggcg gacgcgcggg cctgcgcgaa aggtcgatgc
     1021 tccggctttt gtacgcccgc gcgcggatgc tgagcgaaag cgcctcgacc gacgcggcgg
     1081 cgctggcggc gtgctacggc tacggcctcg cgcgcgaccg gctgttttac agccagaata
     1141 cgggcgtggc gctgctgagc gtcgaactgg cgctgcgcct gaacggctgc cgcctgacgg
     1201 cggacgacac cgcctgtttc ctgagcctga gcctggccgg acagggcgag ccgggcgccg
     1261 aatccttcgc gggctggata cgcggccaca tcgccgaaca ggcgcgcgac gccgcctgag
     1321 cctgacaaca ttgtcaggcg gaaaaacctt tcaaatcgcg aaaccttagc cgcccaaacc
     1381 tgtccattgt cataacgccg tcttgcgaag gcggcgcgcg catggtacgg ctgcgccacc
     1441 ccccatcgcc acccgcgccg tcccatgccc gacacctccc ccgactcccc ctctcccgac
     1501 gcttccgacc gtaagcccac cattatcgac gtcgccgaac gcgcgggcgt ctccatcaag
     1561 accgtgtcgc gcgtgatcaa caacgagcgc cacgtcgcag cggcgacgcg cctgcgggtg
     1621 cgcgaggcgg tcgaggcgct gggcttcgag gccaacgcct cggcgcgcgg catgcgccgc
     1681 cgcgccgccg accgttcgtg ggtgatcgcc cagctttacg gcgatccggg cggggtttac
     1741 accgccaata tccagttcgg cctgctcgaa cgctgtcagt atttcggcta ccatctggcg
     1801 gtcgaggagc tggactatca gaacccgaac gtcgagcggc gcgcgcgcga cctgatcccg
     1861 cggctgcggc ccgacggggc ggtgctgacc gcgccgctga ccgacgacgc gatcattctc
     1921 gacgtgctgg acgaggcggg cgtgccctat gtgcgcgtca cgccgctgaa ggaagac
//
LOCUS       DASVLL010000733         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_267947,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000733 DASVLL010000000
VERSION     DASVLL010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267947"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF402_12020"
     CDS             <1..342
                     /locus_tag="VF402_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801071.1"
                     /translation="LSKRATGKTLYILDEPTTGLHFEDTRKLLEVLHELTDNGNTVVV
                     IEHNLDVIKTADWLLDFGPEGGDGGGEIVARGTPEAVADNPASWTGKYLKEMLAKHRQ
                     RRPASLAEPAE"
     gene            complement(448..1416)
                     /gene="secF"
                     /locus_tag="VF402_12025"
     CDS             complement(448..1416)
                     /gene="secF"
                     /locus_tag="VF402_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7801072.1"
                     /translation="MQWPLIKNLPHEFHFRFTKYAPVAAVISGVAILASAAVIFFMGL
                     NLGTDFAGGNVIEVQTPGPAPLAELRARMPSFGASDVTVQSSGSNDVIIKFKLHEGEQ
                     TEGVEAVKTRLSAAFPGVSFKRTEVVGPKVSGELLRSGLMALGLALLLMLVYIWFRFP
                     SGLQFGAGAVIAILHDLVLTIGIMAALQIEFSMTSIAALLTVVGYSMNEKVITFDRLR
                     ENLRKFKRTPLSDIINLSENERLSRTIITGSTAVLALAGMLFLGGPTLFPLVFAMVFG
                     IIVGTYSSIYVALPLILIWGVNRNDEDAEIFDMGGFKGGKKPKDLP"
     gene            complement(1431..3035)
                     /gene="secD"
                     /locus_tag="VF402_12030"
     CDS             complement(1431..3035)
                     /gene="secD"
                     /locus_tag="VF402_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7801073.1"
                     /translation="MINLSRWKIGVVVLAVLLGVLFTLPNFLPASVRDSIKFLPNKTL
                     NLGLDLQGGSYLLVGVDTDALFKERVNNLTEDVRNTLNDKGIAFSGLGTAAQGSGQGV
                     TVHIDNAAQMDAAYAALTPLASPLPKNPSQKDLSVKRPGNQTITLILTDEGVRETASR
                     AVDTSIEIVRRRIDNLGTKEPSITRQGATRIVVEAPGESDPEQLKRIIGQTAKLTFQM
                     VDNTVTQADIAAGRIPPGSEALPYEEANNQMIVVRKAVLVSGNNLTKATVGVDQYQRP
                     AIDFTFDSTGSQRFGKATAENIGKQFAIILDGKVVSAPTIQGAIPGGHGQITGSFTPQ
                     QASDLVAVLNGGALPAPLKFEEQRTVGAELGADSVAKGELSTVIAFGVIVLFMFLAYG
                     FVFGGVSIAALLVNLLLMLACMTAFQATLTLPGVAGLILSLAVAVDANVLIYERIRDE
                     ERTGHKPAAAMNVGFQRAIVSIMDANVTSLISALIMFMFGAGPVRGFAWTLMIGVFTS
                     VFSAILVSQVLLGWWFKSARPKKLPI"
     gene            complement(3084..3428)
                     /gene="yajC"
                     /locus_tag="VF402_12035"
     CDS             complement(3084..3428)
                     /gene="yajC"
                     /locus_tag="VF402_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7801074.1"
                     /translation="MFATPAYAQTAGAAAPGGDLLTMLPMLIGFIAIMYFFMIRPQQQ
                     QAKRHKAMLAAMKRNDTVVLSSGIVGKIVRVEEAEVMVEIATGVSVRVVKSMITEIRA
                     KGEPAPANDKAA"
     gene            complement(3632..>3778)
                     /locus_tag="VF402_12040"
     CDS             complement(3632..>3778)
                     /locus_tag="VF402_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7801075.1"
                     /translation="NERVTKGEVIGTVGQTGVIDKPQLHFEVRYTPSTDIARPIDPTL
                     VLPK"
BASE COUNT          709 a         1184 c         1237 g          648 t
ORIGIN      
        1 ctgtcgaaac gcgccaccgg caagacgctc tacatcctcg acgagccgac caccggcctg
       61 catttcgagg atacgcgcaa gcttctggag gtgctgcacg aactgaccga caacggcaat
      121 acggtcgtgg tgatcgagca caatctcgac gtcatcaaga ccgccgactg gctgctcgac
      181 ttcggccccg aaggcggcga cggcggcggc gaaatcgtcg cgcggggaac gcccgaagcc
      241 gtggccgaca acccggcgag ctggaccggc aagtatctga aagagatgct ggccaaacac
      301 cgccagcggc gacctgcgtc tctggcggaa ccggcggaat aggtttcctc ccttttcggg
      361 tctttcccgg cccagaaaca aacccagaaa caaaaaagcg cggaagcttg aaacttccgc
      421 gctttttcat tggcttgtcc gcaaatccta cggcagatcc ttcggcttct taccgccctt
      481 gaagccgccc atgtcgaaga tctcggcgtc ctcgtcgttg cggttgacgc cccagatcag
      541 gatcagcggc agcgcgacat atatcgacga ataggtgccg acaatgatgc cgaacaccat
      601 ggcgaacacc agcgggaaca gggtcggccc gcccagaaac agcatcccgg ccagcgccag
      661 caccgccgtc gaaccggtga tgatggtccg cgacaaccgc tcgttctccg acaggttgat
      721 gatgtcggac aacggcgtgc gcttgaactt gcgcaggttt tcacgcaacc ggtcgaaggt
      781 gatgaccttt tcgttcatcg aatagccgac cacggtcagc aaggcggcga tcgaggtcat
      841 cgaaaattcg atctgcaacg ccgccatgat gccgatggtc agcaccagat cgtgcagaat
      901 ggcgatgacc gcgcccgcgc cgaattgcag gccggacggg aagcggaacc agatatagac
      961 cagcatcagc agcagggcca ggcccagcgc catcaggccg gagcgcagca gttcgcccga
     1021 cactttcggc ccgacgacct cggtgcgctt gaacgacacg ccggggaagg cggcggacag
     1081 gcgggtcttg accgcctcga cgccttcggt ctgctcgccc tcgtgcagct tgaacttgat
     1141 gatcacgtcg ttgctgcccg acgactggac ggtcacgtcc gaggcgccga aactgggcat
     1201 gcgcgcgcgc aactcggcca gcggcgccgg tcccggcgtc tgcacctcga tgacgttgcc
     1261 gccggcgaag tcggtgccga ggttcaaccc cataaagaag atgaccgcgg cgctggccag
     1321 aatggcgacg ccggagatca ccgccgccac cggcgcgtac ttggtgaagc ggaaatggaa
     1381 ttcgtgggga agatttttaa tcagaggcca ttgcatggga cagccgcacc ttaaatcggg
     1441 agtttcttcg gacgggcgga cttgaaccac cagccgagca gaacctggct gaccaggatc
     1501 gcggaaaaga cggaggtgaa cacgccgatc atcagcgtcc acgcgaagcc gcgcaccgga
     1561 ccggcgccga acatgaacat gatcagcgcc gagatcagcg aggtcacgtt ggcgtccatg
     1621 atcgacacga tggcgcgctg gaagccgaca ttcatggcgg cggcgggctt gtggcccgtg
     1681 cgctcctcgt cgcgtatgcg ctcgtagatc agcacgttgg cgtcgacggc cacggccagg
     1741 ctgaggatca ggcccgccac gccgggcagg gtcagggtcg cctggaaggc ggtcatgcag
     1801 gccagcatca gcagcaggtt gaccagcagg gccgcgatcg acacgccgcc gaacacgaag
     1861 ccgtaggcca ggaacatgaa cagcacgatc acgccgaagg cgatcacggt cgacagttcg
     1921 cccttggcca ccgagtcggc gcccagctcg gcgcccaccg tgcgctgctc ctcgaacttc
     1981 agcggcgcgg gcaaggcgcc gccgttcagc acggccacga ggtcggacgc ctgctgcggc
     2041 gtgaagctgc cggtgatctg gccgtggccg ccgggaatgg cgccctggat ggtcggggcc
     2101 gacaccacct tgccgtcgag gatgatggcg aactgcttgc cgatattttc cgccgtcgcc
     2161 ttgccgaacc gctgcgagcc ggtggagtcg aaggtgaagt cgatggccgg gcgctgatac
     2221 tgatcgacgc cgacggtcgc cttggtcagg ttgttgcccg acaccagcac cgccttgcgc
     2281 accacgatca tctgattgtt ggcttcctca tacggcagcg cctccgatcc cggcgggatg
     2341 cggcccgcgg cgatgtcggc ctgggtgacg gtgttgtcga ccatctggaa ggtcagcttg
     2401 gcggtctggc cgatgatgcg cttgagttgc tccgggtcgc tttcgcccgg cgcctcgacc
     2461 acgatgcggg tcgcgccctg acgggtgatc gacggctcct tggtgccgag attgtcgatc
     2521 cggcggcgca cgatctcgat cgaggtgtcg accgcgcgcg aggcggtttc gcgcacgcct
     2581 tcgtcggtca ggatcagggt gatcgtctgg ttgccggggc gcttgaccga cagatccttc
     2641 tggctcggat tcttcggcag cggcgaggcc agcggcgtca gcgccgcata ggccgcgtcc
     2701 atctgcgccg cgttgtcgat atggacggtg acgccctgcc cggaaccctg agccgccgtt
     2761 cccagcccgc tgaacgcgat gcccttgtcg ttcagcgtgt tgcgcacgtc ctcggtcagg
     2821 ttgttcaccc gctccttgaa cagcgcgtcg gtgtcgactc cgaccagcag gtaggagccg
     2881 ccttgcaggt cgaggccgag gttgagcgtc ttgttcggca ggaactttat ggaatcgcgg
     2941 acgctggccg ggaggaagtt cggcagggtg aacagcacgc ccaaaaggac ggccagaacc
     3001 accacgccga ttttccagcg ggaaaggttg atcatgtcgg ttcgctcagt ctttttaacg
     3061 gtcggggcgg ttcaggcggc cgctcaggcg gctttatcgt tggccggcgc gggttcgccc
     3121 ttggcgcgga tctcggtgat catcgacttg acgacgcgga cgctgacgcc ggtggcgatc
     3181 tcgaccatga cctcggcctc ctcgacgcgg acgatcttgc ccacgattcc cgacgacagc
     3241 acgaccgtgt cgttgcgctt catcgccgcc agcatcgcct tgtggcgctt ggcctgctgc
     3301 tgctgcgggc ggatcatgaa gaagtacatg atggcgataa agccgatcag catcggcagc
     3361 atggtgagca gatcgccgcc gggagcggcg gcgccggcgg tctgggcgta ggcgggggta
     3421 gcgaacacaa gcgtctcctg ggagtcgtta ttggcagtcg ttataaggat tcagccgcaa
     3481 agcgcgcttc gggcaaaaat agggttcgcc atacataggc gcgcgcagga aaaggtcaat
     3541 gagataattg ccacaaggaa aaagcggtta ttccataaga taaagcgcgg cccggccgcg
     3601 ggccgcgcat cggcgcgcgc cgcaggcggc cttatttcgg cagcaccagc gtcggatcga
     3661 tcggacgggc gatgtcggtc gacggcgtat agcgcacctc gaaatgcagt tgcggcttgt
     3721 cgatcacgcc ggtctggccg accgtgccga tcacctcgcc cttggtgacg cgctcatt
//
LOCUS       DASVLL010000734         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_50529, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000734 DASVLL010000000
VERSION     DASVLL010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50529"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VF402_12045"
     CDS             <1..408
                     /locus_tag="VF402_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEX7801076.1"
                     /translation="KCLTLRSPMVATITGEGGSGGAIAIAAANRVLILEHSIYSVISP
                     EGAASILWKDGARAKEAADRMKITAPDLLKMGIVDRIVEEPAGGAHSDTAAMIRSLGN
                     ALDEELRALSFMTPDQLVAQRAERFYEIGRKGF"
     gene            508..1164
                     /locus_tag="VF402_12050"
     CDS             508..1164
                     /locus_tag="VF402_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801077.1"
                     /translation="MRIPVYAAAIAAALCLCGQAQAAEGVFAGARVSTLGVGGEFGVD
                     LNSHWTARALVNGYDWDYKTTNDDIRYNGKLNLASWGGQIDYRFVADGPLYVTAGLYS
                     NDNKIHATARPTSTTDIGGVPFTPEQIGTLTARGRFSDTAPYLGIGARWPIGPMQISL
                     EAGAYFQGRAHVALTSDGTYAGDPAYQAALEKERRDLQNDVDDYKTYPVVALGLRYGF
                     "
     gene            complement(1152..2057)
                     /locus_tag="VF402_12055"
     CDS             complement(1152..2057)
                     /locus_tag="VF402_12055"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7801078.1"
                     /translation="MNAIMSRFDFVQTFLNTLWVQTRQYWRHLAEKPDFFGHIGWAAV
                     ILLVTLVVANMASNAVRRAARRLVHKDGDRTLLEFFSQIVRWLVLIFGLVMCLNRLGV
                     QTASLLTVLGAASLAIGLALQNTLSNVAAGLMILLNKPYRIGDVVRVGEVQGTVHRLG
                     VFSTEINNTDGVRVFVPNTKVFSNEIVNITNNATMKVELVIGVDYDSDLPFVAGLLKD
                     IADRQPGRLAAPAPVVGYTDFADSSINAKVLIWTLPSQTMSAKSALIMDIKAEFDKHG
                     INIPFPHQVTVQPAAPLPQPTPQNP"
     gene            complement(2059..2547)
                     /locus_tag="VF402_12060"
     CDS             complement(2059..2547)
                     /locus_tag="VF402_12060"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX7801079.1"
                     /translation="MQPDDETLMRAALAQARNAAGAGEAPIGALIYDPSSKTIVTAQR
                     NSPIALSDPCAHAEILALRAAGRARGNYRLTGLWLYATLEPCAMCAGAISHARIGRLI
                     YGADDAKGGAVAHGPQFFAQPTCHWRPEVTAGVLADDSAALLRDFFRARRKKAQPAPD
                     EA"
     gene            2609..>4025
                     /locus_tag="VF402_12065"
     CDS             2609..>4025
                     /locus_tag="VF402_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7801080.1"
                     /translation="MTEDSNAGERDDAAPNTPQPDAGAPQAKTFKPRADKGRDSKGRD
                     AKPAPKPTERIAKVLARAGLASRREVERLIGLGKIAVNGRILDTPAVLVKATDVITVD
                     GQVIGKAEPTRLWRYHKPVGLVTTHRDPEGRSTVFDKLPAGLPRVISVGRLDLNSEGL
                     LLLTNDGELARALEKPSSGWVRRYRARALGHTTQARLDRLKDGTTVDGVIYGPMEASL
                     DKVAEKADGRANVWLTVSITEGKNREVRKVLESIGLKVNRLIRLAYGPFQLGNLEPGD
                     VEEVGPRVIRELLGDVVDMRNLPPEGASQTRTPGRPSHAATGADGKPAGRRAGGDRYG
                     ANKSDGGERKAWKPRREFDDRREFKERDFDQRPRFEGDGESRDRRPARSFENRDFKGP
                     RRDFGDRPKRDGEWKPRPERSEGQGRPRPERGEGQNRTRPERSEGQWKTRAERPEGQW
                     KPRGERPERGERPQARKLDEAG"
BASE COUNT          712 a         1341 c         1366 g          606 t
ORIGIN      
        1 aagtgcctga ccctgcgcag ccccatggtg gccaccatca ccggcgaagg cggctccggc
       61 ggcgccatcg ccatcgccgc cgccaaccgc gtgctgattc tcgaacattc gatctacagc
      121 gtgatctcgc ccgaaggcgc cgcctcgatc ctgtggaagg acggcgcgcg cgccaaggag
      181 gccgccgacc gcatgaagat caccgcgccg gacctgctga aaatgggcat tgtcgaccgc
      241 atcgtcgagg agccggcggg cggcgcccat tccgacacgg cggcgatgat ccgcagcctg
      301 ggcaacgcgc tggacgagga attgcgcgcg ctcagcttca tgacgccgga ccagttggtc
      361 gcgcagcgcg ccgagcgctt ctacgagatc ggccgcaagg gtttttaact cccttattct
      421 ccgctgattt ttccgcgccc cgatccgcct tgtccccgac gcgccggacg ctaccctctg
      481 ccgcgcacag caaggagagg tcagatcatg cgtattccgg tttatgccgc cgccatcgcc
      541 gcggccttat gtctctgcgg gcaggcgcag gccgccgagg gcgttttcgc gggcgcgcgc
      601 gtctcgaccc tgggcgtcgg cggcgagttc ggcgtcgatc tcaattcgca ctggacggcg
      661 cgcgcgctcg tcaacggtta cgattgggat tacaagacca ccaacgacga catccgctat
      721 aacggcaagc tgaacctggc ctcgtggggc ggccagatcg attaccgctt cgtcgccgac
      781 ggcccgcttt acgtgacggc gggcctctac agcaacgaca acaagatcca cgccacggcg
      841 cgcccgacct cgacgaccga catcggcggc gttccgttca cgccggagca gatcggcacg
      901 ctgaccgcgc gcggcaggtt cagcgacacg gcgccctatc tggggatcgg cgcgcgctgg
      961 cccatcggac cgatgcagat cagcctcgaa gcgggcgcct attttcaggg ccgcgcccat
     1021 gtcgccctga cctcggacgg aacctacgcc ggcgatccgg cctatcaggc cgcgctggaa
     1081 aaggagcgcc gcgatctgca gaacgatgtc gacgactaca agacctatcc ggtcgtcgcc
     1141 ctgggcctgc gctacgggtt ttgaggcgtt ggttgaggca gtggggcggc gggctgcacc
     1201 gtcacctgat gcgggaacgg aatgttgatt ccgtgtttgt cgaactcggc cttgatgtcc
     1261 atgatcaggg ccgatttcgc gctcatggtc tggctgggca gcgtccatat cagcaccttg
     1321 gcgttgatgc tgctgtcggc gaagtcggta tagccgacga ccggcgccgg cgcggccaga
     1381 cggcccggct ggcggtcggc gatgtctttc agcaacccgg cgacgaaggg caggtcgctg
     1441 tcgtaatcga cgccgatgac cagctcgacc ttcatcgtgg cgttgttggt gatgttgacg
     1501 atttcgttgg agaacacctt ggtgttcggc acgaagacgc gcacgccgtc ggtgttgttg
     1561 atctcggtcg aaaagacgcc gaggcgatgc acggttccct gcacctcgcc gacgcgcacc
     1621 acgtcgccga tgcggtaggg cttgttgagc aggatcatca gccccgccgc gacgttgctc
     1681 agcgtgttct gcaaggccag gccgatggcc agagacgccg cgcccagcac ggtcagcagc
     1741 gacgccgtct gcacccccag ccggttcagg cacatgacca gcccgaagat cagcaccagc
     1801 cagcgcacga tctgcgagaa gaactccagc agcgtgcggt cgccgtcctt gtgcaccagc
     1861 cgcctggccg cgcgccggac cgcgttggag gccatgttgg cgacgaccag cgtgaccagc
     1921 aggatcaccg ccgcccagcc gatatggccg aagaagtcgg gcttttcggc cagatgccgc
     1981 cagtattgcc gcgtctgaac ccacagcgtg ttcaggaagg tctgcacaaa gtcgaaacgg
     2041 ctcatgatgg cgttcatatc aggcctcgtc cggcgcgggc tgggcctttt tgcggcgcgc
     2101 cctgaaaaaa tccctgagca gggctgcgct gtcgtcggcc agcacgcccg ccgtcacctc
     2161 cgggcgccag tggcaggtcg gctgcgcgaa gaattgcggt ccgtgcgcga ctgcgccgcc
     2221 cttggcgtcg tcggcgccgt agatcagccg cccgatgcgc gcatggctga tcgcgcccgc
     2281 gcacatggcg cacggctcca gcgtcgcgta cagccaaagc ccggtcagcc ggtaattgcc
     2341 gcgcgcccgc cccgccgcgc gcagggccag aatctcggcg tgcgcgcacg gatcgctgag
     2401 cgcgatcggc gaatttcttt gcgctgttac aatggtttta cttgacggat cgtagataag
     2461 agcgccaatc ggcgcctcac cggccccggc ggcgtttcgc gcctgggcca gcgccgcgcg
     2521 catcagggtt tcgtcgtcag gctgcataga gcaggaaaag acgtttcccg cgtccggcgt
     2581 caagtcattt cagagaaaga cgaacatcgt gaccgaggac agtaacgctg gagagcgtga
     2641 cgacgccgcc ccgaatacgc cgcaaccgga cgccggcgcc ccccaggcaa agactttcaa
     2701 gccccgcgcc gataaaggca gggacagcaa gggcagggac gccaaaccgg ccccgaaacc
     2761 caccgagcgc atcgccaagg tgctggcgcg cgcggggctg gcctcgcgcc gcgaggtcga
     2821 gcggctgatc gggctgggca agatcgcggt caacggacgc atcctcgaca cgcccgccgt
     2881 gctggtcaag gcgacggacg tgatcacggt ggacggtcag gtgatcggca aggccgagcc
     2941 gacccgcctg tggcgctatc acaagccggt cggtctggtg accacgcacc gcgacccgga
     3001 agggcgctcg accgtgttcg acaaactgcc cgccgggctg ccgcgcgtga tcagcgtcgg
     3061 gcggcttgac ctcaattccg aagggctgtt gctgctgacc aatgacggcg agctggcgcg
     3121 cgcgctggaa aagccgtcgt cgggctgggt gcggcgttat cgcgcccgcg cgctgggtca
     3181 caccacgcag gcgcggctgg accggctcaa ggacggcacg accgtcgacg gcgtgatcta
     3241 cgggccgatg gaggcttcgc tcgacaaggt ggcggaaaag gccgacgggc gcgccaatgt
     3301 ctggctgacc gtgtcgatca ccgagggcaa gaaccgtgaa gtgcgcaagg tgctggaatc
     3361 gatcgggctg aaggtcaacc gcctgatccg gctggcctac gggccgttcc aactcggcaa
     3421 tctggagccg ggcgacgtcg aggaggtcgg gccgcgcgtg atccgcgaat tgctgggcga
     3481 cgtggtcgac atgcgcaacc tgccgccgga aggcgcttcg cagacccgca cgccgggccg
     3541 cccgtcgcac gccgccacgg gcgcggacgg caaaccggcg ggccgtcgcg cgggcggcga
     3601 ccgctacggc gcgaataagt cggacggcgg cgagcgcaag gcgtggaaac cgcgccgcga
     3661 attcgacgac cgccgcgagt tcaaggagcg cgatttcgac cagcgtccgc gcttcgaagg
     3721 cgacggcgaa agccgcgacc gccgcccggc ccgttcgttt gaaaaccgtg acttcaaagg
     3781 cccgcgccgc gatttcggcg acaggccgaa gcgcgacggt gagtggaagc cccgccctga
     3841 gcgtagcgaa ggacaaggca ggccccgtcc tgagcgtggc gaagggcaaa acaggactcg
     3901 cccggagcgt agcgaaggac aatggaaaac tcgcgctgag cgccccgaag gacaatggaa
     3961 gccgcgcggc gaacgtcccg aacgcggcga gcgtccgcag gcgcgcaagc tggacgaggc
     4021 gggcg
//
LOCUS       DASVLL010000735         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_50595, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000735 DASVLL010000000
VERSION     DASVLL010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50595"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..781
                     /locus_tag="VF402_12070"
     CDS             149..781
                     /locus_tag="VF402_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7801081.1"
                     /translation="MTQLRSAISVAAAFGALALLAACAKPAPKPVADGDMDLGNPNAK
                     VTIVEYASVTCPHCAEFNKDVMPQLMAKYITPGKVHYVYREFLTPPNDVSAAGILLAR
                     CAGKANYFKVIDDIWAAQGQMFADGTGANAQPVLRQIGARYGIEGKAFDQCVTDPKGL
                     ARIQDNIDKYMKQDGVNSTPTFIVNGKRLDRHSGGISDFDDVLQPLLQGK"
     gene            785..1408
                     /locus_tag="VF402_12075"
     CDS             785..1408
                     /locus_tag="VF402_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7801082.1"
                     /translation="MRAALPTLALTVVLAAAAVTAQAADLPPQLARVAPDDHVMGKAD
                     APVTVIEYGSVACPACAAFNESVFPQIKAKYIDTGKARYVFRPMLTGSPAIAVAGTRM
                     AECAGKDRYFAVVDAMMRGQKEYYAWGESNIIAKPILNRIAASFGMNEAAFNACAGDA
                     AGLARLQKAHQAALDAGVTSTPTLYVNGKQVEHHDLASVEAAIKTAR"
BASE COUNT          312 a          531 c          485 g          240 t
ORIGIN      
        1 tcaacgacaa gatgacgcgc tacatgagcg acgacaaggt cgaccgcacc ccgaccttct
       61 tcgtcaacgg caagccgctc accctcgtca agggcgacat cagcgacttc gacaacgcca
      121 tccagcccct gctgaaataa ggccgcacat gacccagctc cgctccgcca tttccgtcgc
      181 cgccgccttc ggggcgctgg ccctgctggc cgcctgcgcc aagcccgcgc ccaagcccgt
      241 ggccgacggc gacatggacc tcggcaatcc caacgccaag gtgacgatcg tggaatacgc
      301 ctcggtcacc tgcccgcact gcgccgagtt caacaaggac gtgatgccgc aactgatggc
      361 gaaatacatc acgccgggca aggtgcatta cgtctatcgc gaattcctca ccccgccgaa
      421 cgacgtgtcg gcggcgggca tcctgctggc gcgttgcgcg ggcaaggcca actatttcaa
      481 ggtcatcgac gacatctggg ccgcgcaggg ccagatgttc gccgacggca cgggcgccaa
      541 cgcccagccg gtgctgcgcc agatcggcgc caggtacggc atcgagggca aggcgttcga
      601 ccagtgcgtg accgatccca aggggctggc gcggattcag gacaatatcg acaagtacat
      661 gaagcaggac ggcgtcaatt cgaccccgac cttcatcgtc aacggcaagc gtctcgaccg
      721 ccattccggc gggatcagcg atttcgacga cgtgctgcaa ccgctgttgc aaggcaagta
      781 ggatatgcgc gccgctctgc cgaccctcgc cctgacagtc gttttggctg ccgccgccgt
      841 cacggcgcag gcggcggacc tgccgccgca actggccagg gtcgcgccgg acgatcacgt
      901 catgggcaag gccgacgcgc cggtgaccgt gatcgaatac ggctcggtcg cctgcccggc
      961 ctgcgcggcg ttcaacgaaa gcgtctttcc gcagatcaag gcgaaatata tcgacaccgg
     1021 caaggcgcgt tacgtcttcc gcccgatgct gaccggctcg cccgccatcg cggtcgcggg
     1081 cacgcgcatg gccgaatgcg cgggcaagga caggtatttc gctgtggtcg acgccatgat
     1141 gcgcggccag aaggagtatt acgcctgggg cgagagcaac atcatcgcca agccgatcct
     1201 gaaccgcatc gccgcctcgt tcggcatgaa cgaagcggcg ttcaacgcct gcgcgggcga
     1261 cgcggcgggg ctggcgcggc tgcaaaaggc gcatcaggcg gccctcgacg cgggcgtgac
     1321 ctcgacgccg accctctacg tcaacggcaa acaggtcgaa caccacgatc tggccagcgt
     1381 agaggctgcc atcaagaccg cgcgctaggc cggatcgaca ttcaacgacc atacgatttc
     1441 ctgcgccgcc tatccatggg cgggaaaggg cgtccatagt tggaaggctt aagggattta
     1501 gccacggaaa acacggatga tggcctttgg ccatcttcac ggatcgccgc ttgcgcggcg
     1561 tacacgga
//
LOCUS       DASVLL010000736         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_50670, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000736 DASVLL010000000
VERSION     DASVLL010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50670"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..810
                     /locus_tag="VF402_12080"
     CDS             16..810
                     /locus_tag="VF402_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX7801083.1"
                     /translation="MLRRLILALTLFSACAMPAAAQDYQSSPPRQDATPRDFGPYLGP
                     YRAKVTAKMLEDFGEQYLYAPLNAALPAPRPGEDRVVFLGDSITDRWNLAKFFPGKPY
                     VNRGIGGQITTQMIVRFHADVAALHPKAVVILAGVNDVQGVFQIESEQQIETNYLTLA
                     QLAEANGVIPIFSKITPLNNYTPNAATVLQDRHADELARLNAWLEGFCAGRGYTLIDY
                     GPVLRDGKGLMRADYTTDGVHPTDSAYAVMAPVAEKAIESALAVRR"
     gene            complement(800..1126)
                     /gene="zapA"
                     /locus_tag="VF402_12085"
     CDS             complement(800..1126)
                     /gene="zapA"
                     /locus_tag="VF402_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7801084.1"
                     /translation="MAEVTVRVNNKPYTVGCADGQEGRVTELARLFNEHVEMVVADVG
                     AIGEVRLFLMAALLMIDEMQDLRAQLGEAHSANARINAGAHEMERKAAFAISDAAERL
                     EKLLSV"
     gene            complement(1126..1443)
                     /locus_tag="VF402_12090"
     CDS             complement(1126..1443)
                     /locus_tag="VF402_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801085.1"
                     /translation="MEQGQAGLNAAGAAMGGHLAVAVDRLDKALAALESRVRALQAGE
                     PVPAYDGPADAVRQDDYRKLLEELDVARSHNAELAMAAESAYAALGAAAADIRQLLGE
                     EAA"
     gene            1626..>1935
                     /locus_tag="VF402_12095"
     CDS             1626..>1935
                     /locus_tag="VF402_12095"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273421.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7801086.1"
                     /translation="MADAIRVLSMEAVHRAKSGHQGMPMGMADVATVLWTKFLKYDPK
                     KPDWADRDRFVLSAGHGSMLLYALLHLTGFKSVSMEDIKNFRQWGTPTPGHPEYGHTA
                     G"
BASE COUNT          339 a          699 c          582 g          315 t
ORIGIN      
        1 ggatgaagac ttcccatgct ccgtcgcctg atcctggctc tcaccctgtt ctccgcatgc
       61 gccatgcccg ccgccgccca ggactaccag tcctcgccgc cgcgtcagga cgcgacgccg
      121 cgtgatttcg gcccctatct cggcccttac cgcgccaagg tgacggccaa gatgctggag
      181 gatttcggcg aacaatatct ctatgcgccg ctgaacgccg ccctgcccgc gccgcgaccc
      241 ggcgaggacc gcgtggtgtt tctgggcgac tcgatcaccg accgctggaa cctggcgaaa
      301 ttctttcccg gcaagcccta tgtcaatcgc ggcataggcg gccagatcac cacccagatg
      361 atcgtgcgct tccatgccga cgtcgccgcc ctgcatccga aggcggtggt catcctggcc
      421 ggcgtcaacg acgtgcaggg cgtgtttcag atcgagagcg aacagcagat cgagaccaac
      481 tacctgacgc tggcgcaact ggccgaagcg aacggcgtca tcccgatttt cagcaagatc
      541 acgccgctca acaattacac gcccaacgcc gcgaccgtgc tgcaagaccg ccacgccgac
      601 gaactggcgc ggctcaatgc ctggctggaa gggttctgcg ccgggcgggg ctatacgctg
      661 atcgactatg gcccggtcct gcgcgacggc aagggcctga tgcgcgccga ctacaccacc
      721 gacggcgtcc atccgaccga ttcggcctat gcggtcatgg cgccggtggc ggaaaaggcc
      781 atcgaatcgg cgctggcggt cagacgctga gcagcttctc cagccgctcc gccgcgtcgg
      841 agatcgcgaa ggccgccttg cgctccattt cgtgcgcgcc cgcattgatg cgcgcattgg
      901 ccgaatgcgc ctcgcccagt tgcgcgcgca agtcctgcat ctcgtcgatc atcaaaagcg
      961 cggccatcag gaacagccgc acctcgccga tggcgccgac atcggcgacc accatctcga
     1021 catgctcgtt gaacaggcgc gccagctcgg tgacgcggcc ttcctgaccg tcggcgcagc
     1081 cgaccgtata gggcttgtta ttgacgcgaa ccgtgacctc agccattacg ccgcctcctc
     1141 gccgagcagt tgccggatgt ccgccgccgc cgcgcccagc gccgcatagg cgctttccgc
     1201 cgccatcgcc agctcggcgt tgtgcgaccg cgccacgtcc agctcctcga gcagcttgcg
     1261 gtaatcgtcc tgacgcaccg cgtccgcggg accgtcataa gcgggaaccg gctcgcccgc
     1321 ctgcaaggcg cgcacgcgcg attccagcgc ggccagcgcc ttgtcgagcc ggtcgaccgc
     1381 gacggccaga tggccgccca tcgcggcgcc cgccgcgttc agccccgcct gtccctgctc
     1441 cataccgacc tccttgcgcc gcccggcgta ttttcgatac cgcgtggcgc gaaaaaacct
     1501 acacttgcgc ggcaagttcc gctaaaggca gcggcaaccc atccgttaac agacagtgta
     1561 atagagactc gcccatgacc acaagcgccg ccgtgacgtc cgggagcaaa ccgtcgtcgg
     1621 tcctgatggc cgacgccatc cgcgtcctgt cgatggaggc cgtccatcgc gccaagtccg
     1681 gccaccaggg gatgccgatg ggcatggccg acgtggcgac ggtgttgtgg acgaagttcc
     1741 tcaaatacga tccgaaaaag cccgactggg ccgaccgcga ccgcttcgtg ctgtcggcgg
     1801 gccacggctc gatgctgctc tatgcgcttt tgcacctgac cggcttcaag tcggtcagca
     1861 tggaggacat caaaaacttc cgtcagtggg gaacgccgac gccgggccac ccggaatacg
     1921 gccacacggc gggcg
//
LOCUS       DASVLL010000737         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_375567,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000737 DASVLL010000000
VERSION     DASVLL010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375567"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1393
                     /locus_tag="VF402_12100"
     CDS             308..1393
                     /locus_tag="VF402_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX7801087.1"
                     /translation="MTYGRDADLLKEQIRSFNGQSDTKPIKPTIYANPGDKFVEISAA
                     DQVKSMGAGVNIAGGYDAGYWDGKGKAEFSDDDVRRVAEAGFKTIRVPLMTFRHIADT
                     EGNLDPAYMKRIDHIVDLAIANHLNVILDEHDFEDCAKDANGCAVLLSNVWYDLALHF
                     QTAPNSVMFELLNEPNGQVDAAIWNAWLPDLVATVRQFNPTRNLIVGPVMWNSADQLP
                     NLKLPADPHIIVTFHYYTPMEFTHQGANWAGPQLEKLRNVSWTGTPAQMAAINTTFDA
                     VKAWGAANNRPIFLGEYGTYGKFNPNMDDRAAWTRAVSAAADSRGFARAYWYYQDGDG
                     FGVWDAGKKQWVKPLLKALLPDSPAAQ"
     gene            complement(1454..>2281)
                     /locus_tag="VF402_12105"
     CDS             complement(1454..>2281)
                     /locus_tag="VF402_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7801088.1"
                     /translation="GGAVDGKGNLYFVDHHFQRIYRWSQSRGLEVVSDAPLDPVNLAV
                     DASGRLMVLSSDGPEATVYSLDPWAPTPAVTLIAPTPVAARPGAVTVMPGNIWDNGEF
                     RDQLDSRSYRFTTLAEMFARDMAVAKPMEYVSPDGSLVLPAFRVYKQGPVNFQGWRFS
                     DTLDTYGFVKARPGQPVVLTNSSEARVYRGVLGAGGAVTDLKPLTDRGGESVAVDAKG
                     RLFVANGQIFVYDAAGRPLGRIDTPERPLQLLFGGEDGDTLFILSHHGLYRLNTKGF"
BASE COUNT          450 a          746 c          710 g          375 t
ORIGIN      
        1 cttaacgtcc gaatggcgcg cgcgcgtccg ttgcttaccg atacgcgccc gatttgacac
       61 acctgacgcc ttgcttgcgc atctggcttg attcagctcc ccgccgccgc tagttttaaa
      121 aatgaaaata ataaatcgga cgacgccaaa ggcgggtccg gcacaaaggg agtgaagtca
      181 catgcaatcg aaactcagtc ttcgcagcac attgtttact ggattggccg gctgtctggt
      241 tttggcgtct catgcatatg cgcaagacgg cgttcctacc accggcgcga ccgcagatcc
      301 gacgcatatg acatacggac gtgacgccga tttgctgaag gagcagattc ggagctttaa
      361 cgggcaatcc gataccaaac cgatcaagcc gaccatctac gccaatccgg gcgacaagtt
      421 cgtcgagatc tccgccgccg atcaggtcaa gagcatgggc gcgggcgtca acatcgcggg
      481 cggctacgac gcgggttact gggacggcaa gggcaaggcc gagttcagcg acgacgacgt
      541 ccggcgcgtg gccgaggcgg gcttcaagac catccgcgtg ccgctgatga ccttccgcca
      601 catcgccgac accgaaggca atctcgatcc ggcctatatg aaaaggatcg accacatcgt
      661 cgatctggcc atcgccaatc atctgaacgt cattctggac gaacacgact tcgaggactg
      721 cgccaaggac gccaacggct gcgcggtgct tctgtcgaac gtctggtacg atctggcgct
      781 gcacttccag accgcgccga attcggtgat gttcgagttg ctgaacgagc cgaacggtca
      841 ggtggacgcc gcgatctgga acgcctggct gcccgatctg gtcgcgacgg tgcgtcagtt
      901 caacccgacg cgcaacctga tcgtcggccc ggtgatgtgg aacagcgcgg accagttgcc
      961 gaacctgaaa ctgcccgccg acccgcacat catcgtcacc ttccactact atacgccgat
     1021 ggagttcacc catcagggcg ccaactgggc ggggccgcag cttgagaagc tgcgcaatgt
     1081 gagctggacc ggcacgcccg cgcagatggc cgcgatcaac accaccttcg acgcggtcaa
     1141 ggcgtggggc gcggccaata accgcccgat cttcctcggc gaatacggaa cctacggcaa
     1201 gttcaacccg aacatggacg accgcgccgc ctggacgcgc gccgtctccg ccgccgccga
     1261 ttcgcgcggc ttcgcccgcg cctactggta ctatcaggac ggcgacggct tcggcgtctg
     1321 ggatgcgggc aagaagcagt gggtcaagcc gctgctcaag gccctgctgc ccgacagccc
     1381 cgccgcgcag taaaaccgac aacgaaaaaa cgccggaaac cccggcgttt ttttatggat
     1441 gcgccgcgcc gcttcagaac cccttcgtgt tcagccggta aaggccgtga tggctgagga
     1501 tgaacagcgt atcgccgtcc tcgccgccga acagcagttg cagcgggcgc tccggcgtgt
     1561 cgatccggcc cagcggccgc ccggcggcgt catagacgaa gatctggccg ttggcgacga
     1621 acagcctgcc tttcgcatcg accgcgacgc tttccccgcc ccggtcggtc aggggcttga
     1681 ggtccgtgac cgcgccgccc gcgcccagaa cgccgcgata gacgcgcgcc tctgaactgt
     1741 tggtcaggac gacgggctgg cccggccggg ccttgacgaa gccgtaggtg tcgagcgtgt
     1801 cggagaagcg ccagccctga aaattgacag gcccctgctt atagacccgg aaggcgggca
     1861 gcacgaggct gccgtcgggc gagacatatt ccatcggctt ggccacggcc atgtcgcgcg
     1921 cgaacatctc ggccagcgtg gtgaagcggt agctgcgcga atcgagctga tcgcggaatt
     1981 cgccattgtc ccagatattg ccgggcatga cggtgacggc gccggggcgc gccgcgacgg
     2041 gcgtcggcgc gatcagcgtg accgcgggcg tcggcgccca cggatcgagg ctgtaaaccg
     2101 tcgcctccgg cccgtccgag gacagcacca tcagacgccc cgacgcgtcg acggccagat
     2161 tgaccggatc gagcggcgcg tcgctgacga cttcaaggcc ccgactctgc gaccagcgat
     2221 agatgcgctg aaaatggtga tcgacgaagt agagattgcc cttgccgtcc accgcgccgc
     2281 c
//
LOCUS       DASVLL010000738         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_375786,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000738 DASVLL010000000
VERSION     DASVLL010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375786"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1207)
                     /locus_tag="VF402_12110"
     CDS             complement(2..1207)
                     /locus_tag="VF402_12110"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7801089.1"
                     /translation="MVTQQNFDVIVIGAGAAGLMCAIEAARRGRRALVIDHAAKPAEK
                     IRISGGGKCNFTHLDATPKTYLSQNPPFCISALKRFTPRDFLDRVTKRGIPYYEKTEG
                     QLFCERGAQDIIDMLLDDLRAASGHLWLATAVSDVGRDDGGFRLATARGDLRCGRLVV
                     ATGGLSIPKMGATGFAYDLARRFGHEIVETRAGLVPFMLGEDWLQRLGGLSGVGLSAG
                     IRAGVRAGGAAFRDGFLFTHRGLSGPAVLQISNYWRPGDGVVIDLMPGVDAAQWLRAR
                     KAERPKAALATIAAEALPQRLAERWLEIFALPAQTRMADLPNRTLDAFAAALNRWTLT
                     PMGTEGYRTAEVTLGGVDTRALSSQTMESRIVPGLHFIGECVDVTGWLGGYNFQWAWS
                     SGWAAGQAV"
     gene            1298..1603
                     /locus_tag="VF402_12115"
     CDS             1298..1603
                     /locus_tag="VF402_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX7801090.1"
                     /translation="MTDLATITQKIAAAVGENSGLGKTVKVDFGDAGKILINATDVPN
                     VVSNEDGPADTTIQISIEDLMALNAGALDPMMAFMTGKMKIQGDLSVAQKLAQVVRS"
     gene            complement(1593..2816)
                     /locus_tag="VF402_12120"
     CDS             complement(1593..2816)
                     /locus_tag="VF402_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7801091.1"
                     /translation="MHEAPSNRRRGRGPRRPIASAPVRNDAGAESHPPVYAALDLGTN
                     NCRLMIASLHNGHFRIVEAFSRIVRLGEGLTHSGQLQDAAVERALKALKICAEKIEKR
                     GAARVRAVATQACRIARNGQQFLNRVERETGLKLSIISPEEEARLAVMGCASLLEPRD
                     LDPMPRAALVIDVGGGSTELSWVEIEALQTEPQIAAAPQSAPLSAQRSAQLNALRAVR
                     RAPRPRYWISMPVGVVTLAERFPEPRDGGFQDWFEAMVAAVTQEIRDFAAPEPLRPHF
                     DAGAAYIVGTSGAITSLAGMHLGLERYDRSKVDGLWMGRDEVQGVIERLLHMGHAGRE
                     QEPCIGRDRADLVLAGAAILEAVQRLWPCQRLRVADRGLREGLLLSMAKRKPKRRRRR
                     KPAARREQAEASVSS"
BASE COUNT          498 a         1035 c         1014 g          456 t
ORIGIN      
        1 cctacacggc ctgacccgcc gcccagccgg acgaccacgc ccactggaaa ttgtagccgc
       61 ccagccagcc ggtgacgtcg acgcattcgc cgatgaagtg caggccgggg acgatgcggc
      121 tctccatcgt ctgcgaggag agggcgcgcg tgtcgacccc gcccagcgtg acctcggcgg
      181 tgcgatagcc ttccgtaccc atcggcgtca gcgtccagcg gttcagcgcg gccgcgaagg
      241 cgtcgagcgt ccggttcggc agatcggcca tgcgcgtctg cgcgggcagg gcgaaaatct
      301 ccagccagcg ctcggccagc cgttgcggca gcgcctcggc ggcgatggtg gccagcgcgg
      361 ccttcgggcg ttccgccttg cgcgcgcgca gccattgcgc cgcgtccacg ccgggcatga
      421 ggtcgatgac gacgccgtcg ccgggacgcc aatagttcga gatttgcagg acggcgggac
      481 cggacaggcc gcgatgggtg aacagaaagc cgtcgcgaaa ggcggctccc ccggcgcgga
      541 ccccggcgcg gatcccggcg ctcaggccga cgccggacag gccgcccagt ctttgcagcc
      601 aatcctcgcc gagcatgaac ggaaccagcc cggcgcgcgt ttcgacgatc tcgtgaccga
      661 agcggcgcgc gaggtcgtag gcgaagccgg tcgcgcccat tttcgggatc gacagtccgc
      721 ccgtcgcgac gaccagcctg ccgcagcgca gatcgccgcg cgcggtcgcc agccggaagc
      781 cgccgtcgtc gcgcccgacg tcggacaccg ccgtcgcgag ccagagatgc ccgcttgcgg
      841 cgcgcaggtc gtcgagcagc atgtcgataa tgtcctgcgc gccgcgttcg cagaacaact
      901 ggccctcggt cttttcgtaa tagggaatgc cgcgtttcgt cacgcggtcg aggaaatcgc
      961 gcggcgtaaa gcgcttcagc gccgaaatgc agaagggcgg gttctgcgac aggtaggttt
     1021 tcggcgtggc gtccagatgg gtgaagttgc acttgccgcc gccggagatg cgtatcttct
     1081 cggcgggctt ggcggcgtga tcgatgacca gcgcgcgccg tccgcgccgg gcggcctcga
     1141 tggcgcacat cagcccggcg gcgcccgcgc cgatgacgat tacgtcgaaa ttctgctgcg
     1201 tcaccatatt ttcgccttat gtcgccgcaa tgaacttgcc cggctggaaa cttacgttaa
     1261 ggtcagctat cacacacacg cacaacagga gcggcgcatg accgatctcg caaccatcac
     1321 tcagaaaatc gccgccgccg tgggcgagaa ttccggtctc ggcaagaccg tgaaggtcga
     1381 tttcggcgat gcgggcaaga tcctgatcaa cgccaccgac gtgccgaacg tcgtttccaa
     1441 cgaggacggc cccgccgaca ccacgatcca gatttcgatc gaggatctga tggcgctgaa
     1501 cgccggcgcg ctggacccga tgatggcctt catgaccggc aagatgaaga ttcagggcga
     1561 cctcagcgtg gcgcaaaagc tggcccaggt ggtcaggagc tgacactggc ttcggcctgt
     1621 tcccgacgcg ccgccggttt gcgccggcgg cgccgtttcg gcttgcgttt cgccatcgac
     1681 aaaagcaggc cctcgcgcag gccccggtcg gcgacgcgca ggcgctgaca cggccacagc
     1741 ctttgcacgg cttccagaat cgccgcgccc gccagcacga ggtcggcgcg gtcgcggccg
     1801 atgcacggct cctgctcgcg gcccgcatgg cccatatgca acaggcgttc gatcacgccc
     1861 tgcacctcgt cgcgccccat ccacaggccg tcgaccttcg agcggtcgta gcgctccagc
     1921 cccagatgca tgccggccag agaggtgatc gcgcccgacg tgccgacgat ataggcggcg
     1981 cccgcgtcga aatgcggacg cagcggttcg ggcgcggcga agtcgcgaat ctcctgcgtg
     2041 accgccgcca ccatcgcctc gaaccagtcc tgaaaaccgc cgtcccgcgg ttcggggaac
     2101 cgttcggcca gggtgacgac gccgaccggc atcgaaatcc agtaacgcgg ccggggcgcg
     2161 cggcgcacgg cgcgcagggc gttcaactgc gccgagcgct gcgccgacag gggcgccgac
     2221 tggggcgccg ccgcgatttg aggctcggtc tggagcgcct cgatctccac ccagctcagc
     2281 tccgtcgagc cgccgccgac atcgatcacc agcgccgccc tgggcatggg atcgaggtcg
     2341 cgcggctcca gcagcgaggc gcagcccatg accgccagac gcgcttcttc ctcaggcgag
     2401 ataatcgaca gtttgaggcc ggtttcacgc tcgacgcgat tgaggaactg ctggccgttg
     2461 cgggcgatgc ggcaggcctg ggtcgccacg gcgcgcacgc gggcggcgcc gcgtttctcg
     2521 atcttttcgg cgcagatttt cagcgccttg agcgcgcgct ccacggcggc gtcctgaagc
     2581 tggccgctgt gcgtcaggcc ctcgcccagc cgcacgatgc gcgaaaacgc ctcgacgatg
     2641 cgaaaatggc cgttgtgcag ggaggcgatc atcaggcggc aattgttggt gccgagatcg
     2701 agcgccgcat agacgggcgg atgcgattcg gccccggcgt cgtttcggac cggcgcggac
     2761 gcgatggggc gtcgcgggcc gcggccccga cgccggtttg agggagcctc gtgcatggct
     2821 cataccccgc tgattccgtc ggaaccctga cggtggacgc cgcctgaccg aggatcggcc
     2881 cggttccttt cccggccacc ttaggctgcg cgtgcaaggc tgccaacccg aaatttgccg
     2941 taaaatccgc cgattgtgga tgacgcgtcc gatgcgggcg aaagacccgg cacacgaaaa
     3001 cgc
//
LOCUS       DASVLL010000739         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_268615,
            whole genome shotgun sequence.
ACCESSION   DASVLL010000739 DASVLL010000000
VERSION     DASVLL010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268615"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VF402_12125"
     CDS             <1..529
                     /locus_tag="VF402_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEX7801092.1"
                     /translation="MSLMLKSSGQHMDVAGVFIDLIIWLVLPLVVGHLLRPLIGGFLA
                     RHKKLTQIADRGAILILVYTSFCDSFARHIWSGNRPATLLLVAAATLLLFFLVLNLLL
                     AASKRLGVDAPARAAVVFCGTKKSLATGVPMAALIFANHPSSLGLILLPIMIYHPLQL
                     LICTPLAARWAKQAG"
     gene            complement(680..>1548)
                     /locus_tag="VF402_12130"
     CDS             complement(680..>1548)
                     /locus_tag="VF402_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7801093.1"
                     /translation="RGCYVDAHDALARADYIAVGDLGGGKDRDRVLLGAALTADEVRH
                     LFAGRLETRPVLQRGGSGFRAFEETRLGALTLASRRLDTVPPDLLLEAEAEEVRSKGL
                     KALKFSEYAEGLRARVAFLRAGDAAWPDMSDAALLSGLADWLGPYAAGRSMLSLGADA
                     VTQGLKGMLDPAQQRALDKLAPETLRLPTGSNARIDYAAEGGPRVEARVQELYGVTTH
                     PTVGPGRAPLTLALLSPAHRPIQITKDLPAFWDGSWREVRAEMKGRYPRHVWPENPRE
                     ADPTTRAKPRGT"
BASE COUNT          223 a          588 c          470 g          267 t
ORIGIN      
        1 gatgagcctg atgctgaagt cctccgggca acacatggat gtcgcaggcg tttttatcga
       61 cctcatcatc tggctggtgc tgccgctggt cgtcggccat ctgctgcgcc cgctgatcgg
      121 cggctttctg gcgcggcata aaaagctgac ccagatcgct gaccgcggcg cgatcctgat
      181 cctcgtctac acctcgttct gcgattcctt cgcgcgccat atctggagcg gcaaccgccc
      241 ggccacgctg ctgctggtcg ccgccgcgac gctgctcttg ttcttccttg tgctcaacct
      301 gctgctggcc gcgtcgaagc gcctcggcgt cgacgcgccc gcccgcgccg ccgtggtgtt
      361 ctgcggcacc aagaagagcc tggcgaccgg cgtgccgatg gcggcgctga tcttcgccaa
      421 ccacccgtcc agcctcggcc tgatcctgct gccgatcatg atctatcatc cgctgcaatt
      481 gctgatctgc acgccgctgg ccgcgcgctg ggcgaaacag gccggctaaa ttatattggc
      541 ctcttatagc gcggcgccta tgctggtccc gaatcgcaag gagccaagtt catgaaccgc
      601 gtcacatccc tgatcctgaa actgctgatc ctgccgctga tcggcgtcgc cgtggcgggc
      661 tgcatcacca tctgagagtt tatgtccctc tgggcttggc ccgcgtggtc ggatcggcct
      721 cgcgcgggtt ttccggccag acatggcgcg gatagcggcc cttcatctcg gcgcgcacct
      781 cgcgccacga gccgtcccag aaggcgggca ggtctttcgt gatctggatc ggccggtgcg
      841 cgggcgacag cagggccagg gtcagcggcg cgcgccccgg ccccacggtg ggatgcgtcg
      901 taacgccgta aagctcctgc acccgcgcct cgacgcgcgg cccgccctcc gccgcatagt
      961 cgatgcgcgc gttcgatccg gtcggcagcc ggagcgtttc cggcgccagt ttgtccagcg
     1021 cccgctgctg tgcgggatcg agcattccct tcaggccctg cgtcaccgca tcggcgccga
     1081 ggctcagcat cgaccgcccc gccgcatacg gccccagcca gtcggccagc ccgctgagca
     1141 gcgccgcgtc cgacatgtcc ggccacgccg catccccggc gcgcagaaac gccacccgcg
     1201 cgcgcaagcc ttcggcgtat tcggaaaatt tcagcgcctt caaccccttg gaccgaacct
     1261 cctcagcctc ggcctcaagc agcaggtccg gcggaaccgt gtccaaccgc ctcgacgcca
     1321 gcgtcagcgc gcccagccgc gtctcctcga acgcacggaa ccccgacccg ccgcgttgca
     1381 ggaccggcct cgtctccagc cgcccggcga acagatggcg cacctcgtcc gccgtcagcg
     1441 ccgcccccag cagcacgcgg tcgcggtcct tgccgccgcc gagatcgccg accgcgatat
     1501 agtcggcccg cgccagcgcg tcgtgcgcgt cgacatagca accgcgcc
//
LOCUS       DASVLL010000740         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_51242, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000740 DASVLL010000000
VERSION     DASVLL010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51242"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..292
                     /locus_tag="VF402_12135"
     CDS             14..292
                     /locus_tag="VF402_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801094.1"
                     /translation="MKKMLVIAASLLALTGMSACAEYDYGHGPGRYAAYDLYYDGYYG
                     PVSEGYWGGDGWFWYRDSTRHWRRDDGRHFRRDAYNGYHPYRVHRGRR"
     gene            523..1581
                     /locus_tag="VF402_12140"
     CDS             523..1581
                     /locus_tag="VF402_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7801095.1"
                     /translation="MRGGKPTIDDVAALSGVARATVSRVLNGGPNVRDEVRQRVLEAV
                     EKLQYKVNMQARFLAGGRSQVLALVYASDLDSQPNSFYHAGLELGALRGCAERGFQLL
                     MHTVNQHSLSKQKSILELVDTRRCDGLLLTPPFSDDLDLLGELQARRTPVVCIAAGRE
                     ARTLASGIGIDDEVAGYEITRHVLKLGHRRIGFIKGLEGHLSAEERFAGFRRALREQG
                     LDENDMMTARGTFSFRSGVETTARLLGGPERPTALICANDDMAVGALFTAHKLGLEIP
                     RDLSVTGFDDTPVSEIIWPPLTTVHQPLKLIGARAVEMIVDQINAAKTSATPPAHRYE
                     LAPHRVVMRESADALVKS"
     gene            1658..2125
                     /locus_tag="VF402_12145"
     CDS             1658..2125
                     /locus_tag="VF402_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801096.1"
                     /translation="MPVLGYIRRMDGKAAPAQAKTKRKRRPLADRLEDKRLKYEQTLR
                     QRFNVLMEESGAKPDDPSIEKRARMLLTLRKACLELYPAPKARGEAEMDDRRDGDAGG
                     DRGEDAAMDAWRSLFEHKVSRYHASRSAPAVAEPARRGDDREHADGVEHLGPG"
     gene            2091..>2431
                     /locus_tag="VF402_12150"
     CDS             2091..>2431
                     /locus_tag="VF402_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX7801097.1"
                     /translation="MLTAWSIWGRDEQRPPDGDWHSWLFLGGRGAGKTRAGAEWVKSL
                     LAPGLRIALVGPTLHDVREVMIDGPSGLRSVADPGNRPFYEVSRRRLRWPGGGTAYAF
                     SAEEPERLRGP"
BASE COUNT          417 a          746 c          875 g          393 t
ORIGIN      
        1 aggatgaagc gccatgaaga agatgctggt gatcgccgcg agtctgctgg ccctgacggg
       61 gatgagcgcc tgcgcggagt atgattacgg ccacgggccg ggccggtacg ccgcttacga
      121 tctctattac gacggctatt acggaccggt cagcgaaggc tattggggcg gcgacggctg
      181 gttctggtat cgcgactcga cgcgccactg gcggcgcgac gacggacgcc atttccgccg
      241 cgacgcctat aacggctatc atccgtaccg cgtccatcgc ggccgcagat agtcttaacc
      301 cgcaacctta acctgttcgt cagcgtgcgc ccatgagcat gaccattacg accgccgcca
      361 tcgccgccct gaccgtcatc gtcacgatga tcatggcggt cgcggccatt cttatcgcga
      421 aacagaattg aggcggcgtc acgcgccgcc tgcatcagga agggcttgtc tcggagcgcc
      481 gaaccggcct aaatgagacc gtcaagcaac tttgggattg agatgcgcgg gggaaagccg
      541 acgatcgacg atgtggccgc cttgtcgggg gtggcgcggg cgacggtgtc gcgtgtgctg
      601 aacggcggtc cgaacgtgcg cgacgaggtg cgccagcgcg tgctggaggc ggtcgaaaag
      661 ctgcagtaca aggtcaatat gcaggcgcgc ttcctggccg gggggcgcag tcaggtgctg
      721 gccctggtct atgcgtcgga cctcgatagc cagcccaatt ccttttatca tgcggggctg
      781 gagctggggg cgctgcgcgg ctgcgccgag cgcggttttc agcttctgat gcacacggtc
      841 aaccagcatt cgctgagcaa gcagaagtcg attctcgaac tggtggacac gcgccggtgc
      901 gacgggctgc tgctgactcc gccgttttcc gacgatctgg acctgctggg cgaattgcag
      961 gcgcggcgca cgccggtggt gtgcatcgcc gccggacgcg aggcgcgcac gctggcgtcg
     1021 ggcatcggca tcgacgacga ggtggcgggc tatgagatca cgcggcatgt gctgaagctg
     1081 ggccatcgcc gcatcggctt catcaagggg ctggaggggc acctgtccgc cgaggagcgc
     1141 ttcgccggct tccgccgcgc cctgcgcgag caggggctgg acgaaaacga catgatgacg
     1201 gcgcgcggca cgttcagctt ccgctccggc gtggagacga cggcgcggct gctgggcggg
     1261 ccggagcggc cgacggcgct gatctgcgcc aatgacgaca tggcggtcgg cgccctgttc
     1321 accgcgcaca agctggggct ggagataccg cgcgacctgt cggtgaccgg gttcgacgac
     1381 acgccggtgt cggagatcat ctggccgccg ctgaccaccg tgcatcagcc gctcaagctg
     1441 atcggcgcgc gggcggtcga gatgatcgtc gaccagatca acgccgccaa gacctcggcc
     1501 acgccgcccg cgcatcgtta cgagctggcg ccgcaccgcg tggtgatgcg cgaaagcgcc
     1561 gacgcactcg tcaaaagctg acttatccgc gcggtctgaa tttctttttt taataaaatc
     1621 aatgtcctgt cggaaagttc cggcggggcg tccccctatg ccggtcctcg gctacattag
     1681 gcgcatggac ggaaaagcgg ccccggcgca agccaagacg aagcggaagc ggcgccctct
     1741 ggcggaccgg ctggaggaca agcggctgaa gtacgagcag accctgcgcc agcgcttcaa
     1801 cgtgctgatg gaggagagcg gggcgaagcc ggacgatccg tccatcgaga agcgcgcccg
     1861 gatgctgctg acgctgcgca aggcgtgtct ggaattgtac ccggcgccga aggcgcgagg
     1921 agaggcggag atggatgatc gacgtgacgg ggacgccgga ggagatcgcg gcgaggacgc
     1981 agctatggac gcctggcgat ccctgtttga acataaagta tctcgctacc atgcctcccg
     2041 aagcgctccg gctgtggctg aaccggcacg acgcggcgac gatcgggagc atgctgacgg
     2101 cgtggagcat ctggggccgg gatgagcaaa ggccgccgga cggcgactgg catagctggc
     2161 tgtttctcgg cgggcgcggc gcgggcaaga cgcgcgccgg ggccgaatgg gtgaaaagcc
     2221 tgctggctcc cggtctgcgc atcgccctgg tcgggccgac cctgcacgat gtgcgcgagg
     2281 tgatgatcga cgggccttcg ggcctgcgca gcgtcgccga tccgggcaac cggccctttt
     2341 acgaggtttc gcggcggcgc ctgcgctggc cgggcggcgg gacggcctac gccttttcgg
     2401 cggaggagcc ggagcggctg cgcgggccgc a
//
LOCUS       DASVLL010000741         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_51470, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000741 DASVLL010000000
VERSION     DASVLL010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51470"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /gene="rplU"
                     /locus_tag="VF402_12155"
     CDS             complement(<1..143)
                     /gene="rplU"
                     /locus_tag="VF402_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7801098.1"
                     /translation="MYAVIKTGGKQYRVAAGDLIKVEKLEGEAGAAVTFNDVLMIGDE
                     AGAV"
     gene            441..1544
                     /locus_tag="VF402_12160"
     CDS             441..1544
                     /locus_tag="VF402_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7801099.1"
                     /translation="MPDAATTFQKTPVTVLTGYLGAGKTTLLNRILSENHGKKYAVIV
                     NEFGEIGIDNDLIVGADEEVFEMNNGCVCCTVRGDLIRILSGLMKRQKSKGGGKFDAI
                     IVETTGLADPGPVAQTFFVDEDVKAKTYLDSVTALVDARHFEDALNTDKVVKEQVAFA
                     DNIILNKTDLVSPEKLDEVEARLKGLNPLARITRAERSGVAIDSLLNQHRFDLSKIDA
                     IGEDDHDHHHHDHHDHDHDHGHHHHHNPAHGEAGHVHDEHCAHHINPIHDEDIRSVSL
                     STDKPIDGRRFTMWLDKLLAEKGVDILRAKGILNVQGEDKRLVFQAVHMILEGDLQGE
                     WKPGEPRLSRAVFIGRRLNEQELREGFNATIAA"
     gene            complement(1640..2311)
                     /locus_tag="VF402_12165"
     CDS             complement(1640..2311)
                     /locus_tag="VF402_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX7801100.1"
                     /translation="MKIEDKILDRGAVLLLANAGEQVALVGCELEELDLSDLILNGWS
                     FEDCILKKTKFASAKLEGARFIKCKAAHADFTGAALVEAVFEQGDFNNVRFRGAHVAQ
                     ARFLACKLTGADFNDARSLDATFEGCRLNDARLRGFAFKGLTLKSLDLTHADLRNCDF
                     RDVTFEDCSLRDADLGHARFEGADLRGTDIGGVKLEDARRFKGATISRAQAAELLSEL
                     GLKVR"
     gene            complement(2301..3002)
                     /locus_tag="VF402_12170"
     CDS             complement(2301..3002)
                     /locus_tag="VF402_12170"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEX7801101.1"
                     /translation="MWKPLHPLYNVYRRWFGRYTLDDPRPIQREAPYTYYLPTAVRLE
                     SIQAGDRVQLLFRSEPHGLKYGIERMWVEVEAVTGDDLKGKLLNKPFDMPQLKVGSRI
                     AFKRYNAIDISSDRELPPDSPQVKQYWDRCIVDKCVLYDSVPVYYIYREEPDMAQPDD
                     KDPDSGWRIRGDYRHISDDELEARKASYIALGKVLNADDSWLHLIDSPIGSAFMRNFE
                     TGEYEPYERSVSDED"
     gene            complement(3162..>3681)
                     /locus_tag="VF402_12175"
     CDS             complement(3162..>3681)
                     /locus_tag="VF402_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7801102.1"
                     /translation="VIREIVEKTGAKVDIADDGTIKIAAAEQSKIDAARDWIKSIASE
                     PEVGAIYKGKVVKIVDFGAFVNFFGAKDGLVHISQIKPEKIAKVSDFLNEGDEVKVKF
                     LGFDDRGKTKLSMKVVDQETGEDLTEKLEAERAERNAARAAVGEEPEMETADGGEERS
                     ERPRRPRRPRRD"
BASE COUNT          764 a         1199 c         1016 g          702 t
ORIGIN      
        1 acggcgccgg cttcgtcgcc gatcatgagg acgtcgttga acgtcacggc ggcgccggct
       61 tcgccttcca gcttttcgac cttgatcaga tcgccagccg caacacggta ttgcttaccg
      121 cccgttttga tgaccgcgta catggtcaga accctatgca gtggcgcaag gcatgtgcag
      181 ggttctgcaa ggcttgcgcg ggaaagaaaa ggaaaacgcc ccagaaggcg caaggccttc
      241 gggcgagagg cggcgactta tacagaggaa tgcttgtgag tcaaccgccg cggcatgttt
      301 atttggcgtt ttcgccgcgc cgcttcaagc ctctaagctg tttcgccgca tttgcgcaag
      361 gcgtcgcgac gcggacttga tttcgcgccg caatagcata cttaaaagcc gattataaag
      421 taacactcaa aaggcccgcc atgcccgacg ccgctacaac tttccaaaag actcccgtca
      481 ccgtgctgac cggctatctg ggcgcgggca agacgacgct tctgaaccgc atcctgtcgg
      541 aaaaccacgg caaaaagtac gcggtcatcg tcaacgaatt cggcgagatc ggcatcgaca
      601 acgacctgat cgtcggcgcc gacgaggaag tgttcgagat gaacaacggc tgcgtgtgct
      661 gcacggtgcg cggcgacctg atccgtattc tttcgggcct gatgaaacgc cagaaaagta
      721 aaggcggggg caaattcgac gccatcatcg tcgaaaccac gggcctcgcc gatcccggcc
      781 cggtggcgca gaccttcttt gtcgacgagg acgtcaaggc gaaaacctat ctcgattccg
      841 tcaccgccct ggtcgacgcg cgccatttcg aggacgcgct gaacaccgac aaggtggtca
      901 aggagcaggt cgccttcgcc gacaacatca tcctcaacaa gaccgatctg gtcagccccg
      961 aaaagctgga cgaggtcgag gcgcggctca agggcctgaa cccgctggcg cgcatcaccc
     1021 gcgccgagcg ctccggcgtc gccatcgaca gcctgctgaa ccagcaccgc ttcgacctgt
     1081 ccaagatcga cgcaataggc gaggacgatc acgatcacca ccaccacgat catcatgatc
     1141 acgaccatga ccacggccac caccaccatc acaatccggc gcacggcgag gccggacatg
     1201 tgcatgacga gcattgcgcg caccacatca acccgatcca cgacgaggac atccgctcgg
     1261 tttcgctcag cacggacaag ccgatcgacg ggcgccgctt cacgatgtgg ctggacaagc
     1321 tgctggccga aaagggcgtc gacatcctgc gcgccaaggg catcctcaac gtgcagggcg
     1381 aggacaagcg cctggtcttt caggccgtcc acatgatcct ggaaggcgat ctgcaaggcg
     1441 aatggaaacc cggcgaaccg cgcctctccc gcgccgtctt catcggccgc cgcctgaacg
     1501 aacaggaact gcgcgaaggc ttcaacgcca cgatcgccgc ctgaccatcc ctccctgcgc
     1561 agcggggttt ggcgcagcga aagcactgct ttcgcaaagc catagggagg gaccgcgaag
     1621 cggtgggagg gacctctccc tacctgacct tcaaccccag ctccgacagc aactccgccg
     1681 cctgcgccct tgaaatcgtc gcgcccttga aacgccgcgc gtcctccagc ttcaccccgc
     1741 cgatatccgt gccgcgcagg tccgcgccct cgaaccgcgc atggccgaga tcggcgtcgc
     1801 gcaaggaaca gtcctcgaac gtcacatcgc ggaaatcgca attgcgcagg tcggcatggg
     1861 tcaggtcgag cgatttcagc gtcagcccct tgaacgcgaa cccgcgcagg cgcgcatcgt
     1921 tcaaccggca gccctcgaac gtcgcgtcca gcgagcgcgc atcgttgaaa tccgctccgg
     1981 tcagcttgca ggcaagaaac cgcgcctgcg ccacatgcgc gccgcgaaag cggacattgt
     2041 tgaaatcgcc ctgctcgaac accgcctcga ccaaggccgc gccggtgaaa tcggcgtgcg
     2101 ccgccttgca cttgataaac cgcgcgccct ccagcttagc cgacgcgaac ttcgtcttct
     2161 tgagaataca atcctcgaac gaccacccgt tcaagatcag gtccgacagg tccagctcct
     2221 ccagctcgca accgacaagg gctacctgct cacccgcatt ggccagcaac agcaccgcgc
     2281 cccggtcgag gattttgtct tcaatcttca tctgaaacgc ttctttcata gggctcgtat
     2341 tcgccggttt caaagttccg catgaaggcg gaaccaatcg gcgaatctat caaatgcaac
     2401 cagctgtcat cagcgttaag caccttgccc agtgcaatat agcttgcctt tcgcgcttcg
     2461 agttcatcgt cgcttatatg tcggtaatcc ccccgtatgc gccatccgct gtcgggatct
     2521 ttatcgtcag gctgagccat atccggctcc tcccgataga tgtaataaac agggacgctg
     2581 tcatagagta cgcacttatc gacaatgcac cggtcccagt attgcttaac ctggggggaa
     2641 tccgggggca attctctgtc cgaactgatg tcgatcgcat tatagcgctt gaacgcaatc
     2701 ctgcttccga ctttaagctg aggcatgtca aagggtttgt ttagcaattt tccctttaag
     2761 tcatcgcctg taacggcctc gacctcgacc cacattctct caatgccata tttcaaacca
     2821 tgcggttcgc tgcggaagag gagttgtaca cggtcgcccg cctgaatgct ctctaagcgc
     2881 accgccgtcg ggagatagta agtatatggc gcttcccgct gaatcggacg cggatcatcg
     2941 agcgtataac gtccgaacca tcgcctgtag acattgtata aaggatgaag cggcttccac
     3001 ataccagcga ggataactcc gtcccgttac ggggtcgagg ggtcgatgac ccctcgcctt
     3061 gaaaacaaac gataagggcg gccccttgcg ggaccgccct tatcgtttgt cttgaagaag
     3121 acgaaggggc cattggcccc ttcaccccgt cacggaccga gttagtcccg acgcgggcgg
     3181 cgcgggcggc gcggacgctc gctgcgctcc tcaccaccgt cagcggtttc catctccggc
     3241 tcctcgccga cggcggcgcg ggcggcgtta cgctcggcgc gttcggcctc cagcttttcc
     3301 gttaggtctt cgccggtttc ctgatcgacc accttcatgg aaagcttggt cttgccgcgg
     3361 tcgtcgaagc cgaggaactt caccttgacc tcgtcgcctt cgttcaggaa gtccgacacc
     3421 ttggcgatct tttccggctt gatctgcgaa atgtggacca gaccgtcctt ggcgccgaag
     3481 aagttcacga aggcgccgaa atcgacgatc ttcacgacct tgcccttata gatggcgccg
     3541 acttccggct ccgaggcgat cgacttgatc cagtcgcggg ccgcgtcgat cttcgactgc
     3601 tcggcggcgg cgatcttgat cgtgccgtcg tcggcgatgt cgaccttggc gccggtcttc
     3661 tcgacgatct cgcggatcac c
//
LOCUS       DASVLL010000742         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_51678, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000742 DASVLL010000000
VERSION     DASVLL010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51678"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1422)
                     /locus_tag="VF402_12180"
     CDS             complement(103..1422)
                     /locus_tag="VF402_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7801103.1"
                     /translation="MTQDTSAAARSTTGRYRWGIIALLFAALAINYTHRQMLGLFKGD
                     LETELHWDEETYAGMVFWFQAAYAVGQMLFGRFLDVAGVRVGYAIGYVVWVVAHMATG
                     LVSGVIEFGAARFALGLGESGAFPSSLKAIAEWFPQKERALAAGIFNAGTSIGVMIAA
                     AVVPFIALGEFHLFGHAFHGLGWGWRGSFLFTGALSLIWLVPWFIMYRHPAEQPKLSA
                     DELNYIQSGQSAVVEVEKVGWLKLLTIKETWTYALGKFLIDPIWWLWLFWLPDFLHKN
                     YGLDIASFGLPLIVIYLMADIGSVAGGWLSSTLIHRGVSVNVARKAALFVFAACAVPI
                     LFIHDVHDLWPAVFLIGLACAGHQAFSCNFYTLPSDLFPRAAVGSVLGIGGSAGAVGG
                     MLMSLFVGWILQTTHSYTLIFAIAAFTYLVAAVIIHLLTPKYTAAKI"
     gene            1609..>2164
                     /locus_tag="VF402_12185"
     CDS             1609..>2164
                     /locus_tag="VF402_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX7801104.1"
                     /translation="MNPMKLKYALIGAAALLAGGAGAATLGTNTPALSLTPGRVAALP
                     RQQGASWAAYLARSKAQEAADKAALAAERRGLAQIPPPPQEDHGETMPLDKPDAWYAT
                     AQARHVADVIVSFQTPAGGWGKNQRRDGAPRQRGQAYVADNISKFLGPDDYDLPHDPQ
                     WNYVGTLDNNATTTEMRFLVRVAAR"
BASE COUNT          443 a          694 c          709 g          318 t
ORIGIN      
        1 ttagagcgct ttccgaaaag tgtgacgcac ttttcggatt aaaaagcgcg taaaagcata
       61 aagttagagc gccgatctga tgcaatcaga tcggaaaacg ctctaaatct tcgccgccgt
      121 gtatttcggc gtcaaaaggt ggatgatcac cgccgcgacc agataggtga aggcggcgat
      181 ggcgaagatc agcgtgtagg aatgcgtggt ttgcaggatc cagccgacga acaggctcat
      241 caacatgccg ccgaccgcgc ccgccgaacc gccgatgccc agcaccgagc cgaccgccgc
      301 gcgcgggaac aggtccgacg gcagggtgta gaagttgcac gaaaacgcct gatgccccgc
      361 gcaggccaga ccgatcagga acacggcggg ccacaggtca tgcacgtcgt ggatgaacag
      421 gatcggcacg gcgcaggcgg cgaacacgaa cagggcggct ttgcgcgcca cgttcaccga
      481 cacgccgcga tggatcaggg tcgaggacag ccagccgccc gccaccgagc cgatatcggc
      541 catcaggtag atcacgatca gcggcaggcc gaacgaggcg atgtcgaggc cgtaattctt
      601 gtgcaggaag tcgggcagcc agaacagcca cagccaccag atcggatcga tcaggaactt
      661 gcccagcgcg taggtccacg tctccttgat cgtcagcagt ttcagccagc cgaccttttc
      721 gacctcgacg accgccgact ggccgctctg gatatagttc agctcgtcgg cgctcagctt
      781 gggctgctcc gccggatggc ggtacatgat gaaccacggg accagccaga tcaggctcag
      841 cgcgccggtg aacaggaacg agccgcgcca gccccagccg aggccgtgga acgcatggcc
      901 gaacaggtgg aattcgccca gcgcgatgaa gggcacgacg gcggcggcga tcatgacccc
      961 gatcgaggtg cccgcgttga agatgcccgc cgccagcgcg cgctcctttt gcgggaacca
     1021 ctcggcgatg gccttcaggc tggacggaaa ggcgccggat tcgcccaggc ccagcgcgaa
     1081 gcgcgccgcg ccgaactcga tcacgccgga cacgaggccg gtcgccatgt gcgcgaccac
     1141 ccagacgaca tagccgatgg cgtagccgac gcgcacgccc gccacgtcga ggaagcgccc
     1201 gaacagcatc tggccgaccg cgtaggcggc ctggaaccag aacaccatgc ccgcataggt
     1261 ctcctcgtcc cagtgcagct cggtctccag atcgcccttg aacaggccca gcatctggcg
     1321 gtgggtgtag ttgatggcga gcgccgcgaa cagcagggcg ataatgcccc agcggtagcg
     1381 gcctgtggtg gaccgcgcgg cggcggaagt gtcctgtgtc atctgtgttc ctgtggcctg
     1441 tattctgtga gtcttggcgc tctttccccg gaggcggcgc ctttgtaagt cggaatggag
     1501 catatgccaa acctgacacc ggtagcaact ttaaaatagg taaaacctat tcgcttgaca
     1561 tcgcgatgac accggtagca attaggcggg tcaacaggga taagcacaat gaatccgatg
     1621 aaactgaaat acgccctgat cggcgcggcg gccttgctgg cgggcggcgc cggggcggcg
     1681 acgctgggga cgaatacgcc cgccttgtcg ctgacgccgg gccgcgtcgc cgcgctgccg
     1741 cggcagcagg gcgcttcgtg ggccgcctat ctggcgcgat cgaaggcgca ggaggccgcc
     1801 gacaaggccg ctctggccgc cgagcggcga gggctggcgc agattccgcc gccgccgcag
     1861 gaagaccacg gcgagaccat gccgctggac aagcccgacg cgtggtatgc gacggcgcag
     1921 gcccggcatg tcgcggacgt gatcgtctcg ttccagaccc cggcgggcgg ctggggcaag
     1981 aaccagcgcc gcgacggcgc cccgcgccag cgaggccaag cctatgtcgc tgacaatatc
     2041 tcgaaattcc tcggccccga cgattacgac ctgccgcacg atccgcaatg gaactatgtc
     2101 ggcacgctcg acaacaacgc gaccacgacc gagatgcgct ttctggtccg ggtcgccgcg
     2161 cggg
//
LOCUS       DASVLL010000743         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_52203, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000743 DASVLL010000000
VERSION     DASVLL010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52203"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VF402_12190"
     CDS             <1..479
                     /locus_tag="VF402_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7801105.1"
                     /translation="NLRKVKSEISALEEGDLRHDQVEQIATKLGVSENDVISMNRRMS
                     SPDSSLNAPLRAAEGESEWQDWLVDDKQLSQETVLADAEEYDIRMELLQAAMGELNDR
                     EREILTERRLKDNPTTLEDLAERFGVSRERVRQIEVRAFEKLQKAMVTASREATPA"
     gene            complement(476..1264)
                     /locus_tag="VF402_12195"
     CDS             complement(476..1264)
                     /locus_tag="VF402_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7801106.1"
                     /translation="MSRVLILEGNPAYARMLADMLRVLGAENIVIETDGDAATALLRD
                     FDPQLIVTEYRTAKIDGVAFTRQLRHSRLSCKAVPVLMVKAEVTVDELREARNAGVHE
                     VLRKPFAWQDLLSRLQNVLLKPRDWVEVATYTGPCRRSFNTGDYKGPKKRKGDGGNLR
                     VAVEEAVRLLEASLNLLEEDAAAAMTSIMQQMQVIVPACKVFRNPKFSNTAARIVQDL
                     RNKALSRENLAPQIAAMKEGLGMDKAADARRLAEELFGGDEKAG"
     gene            complement(1585..>2725)
                     /locus_tag="VF402_12200"
     CDS             complement(1585..>2725)
                     /locus_tag="VF402_12200"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX7801107.1"
                     /translation="KLSLLPSGVVQGKLSIVGNGVVIDPWALFAEIARVEAQGLSITP
                     DLLVIADNACLILPIHSELDLAREAAAGAGKIGTTGRGIGPAYEDKVGRRAIRVADLE
                     DFDALGAKIDRLLAHHNPLRQGLGLPDVDVAGLLDKLREIAPRLLSHVKPAWYVLDKA
                     RTEGKRILFEGAQGALLDIDHGTYPFVTSSNTVAGQAAAGSGMGPKSVGYVLGILKAY
                     TTRVGSGPFPTELFDEVGKGIAERGHEFGTVTGRARRVGWLDAVLARQSIAINGIDGI
                     ALTKLDILDGLDVIKVCVGYKVGDKVLDYLPAGFKAQAGIEPVYEEMEGWSQSTQGAR
                     SWKDLPGAAVKYVRRIEELIGAPVALLSTSPEREDTILMRDPFQD"
BASE COUNT          475 a          915 c          873 g          462 t
ORIGIN      
        1 tcaacctgcg caaggtgaag tcggaaatct cggcgctgga ggaaggcgac ctgcgccacg
       61 accaggtcga gcagatcgcc accaagctgg gcgtgtcgga aaacgacgtg atcagcatga
      121 accggcgcat gtcctcgccc gattcctcgc tgaacgcgcc gttgcgcgcc gccgagggcg
      181 agtcggaatg gcaggactgg ctggtcgacg acaagcaatt gtcgcaggaa accgtgctgg
      241 ccgacgccga ggagtacgac atccgcatgg agttgttgca ggcggcgatg ggcgaactca
      301 acgaccgcga gcgcgaaatc ctgaccgagc gccgcctcaa ggacaatccg accacgctgg
      361 aagacctggc cgaacgcttc ggcgtatcgc gcgagcgcgt gcgccagatc gaggtgcgcg
      421 ccttcgaaaa gctgcaaaaa gcgatggtca cggccagccg cgaagcgacg cccgcctaac
      481 cggccttctc gtcgccgccg aacagctcct cggccagacg gcgcgcgtcg gcggccttgt
      541 ccatgcccaa gccttccttc atcgccgcga tctggggcgc caggttttcg cgcgacagcg
      601 ccttgttgcg caggtcctgc acgatgcgcg cggcggtgtt gctgaatttc gggttgcgga
      661 agaccttgca ggccggaacg atgacctgca tctgctgcat gatcgacgtc atggccgccg
      721 ccgcgtcctc ttcgagcagg ttcagcgacg cctccagcag ccgcaccgcc tcctcgaccg
      781 cgacgcgcaa attcccgccg tcgcccttgc gcttcttcgg ccccttgtaa tcgccggtgt
      841 tgaagctgcg gcggcacggg ccggtatagg tcgcgacctc gacccagtcg cgcggtttca
      901 gcaacacgtt ttgcaggcgg ctgagcagat cctgccacgc gaacggcttg cgcagcacct
      961 catgcacgcc ggcgttgcgc gcctccctca actcgtccac cgtcacctcg gccttgacca
     1021 tcagcaccgg aacggccttg cagctcaggc ggctgtggcg caactggcgc gtgaaggcga
     1081 cgccgtcgat cttcgccgtc ctgtattccg tgacgatcag ttgcgggtcg aaatcgcgca
     1141 agagggccgt ggccgcgtcg ccgtccgtct cgatgacgat attctccgcg cccagcaccc
     1201 gcaacatgtc ggccagcatc cgggcatagg ccggattgcc ttcgagaatc agcacacgac
     1261 tcatgtgccg tcgcagacgg gcctgcaatt tgggatcgag agcgaacata ggcgaccaac
     1321 aaattctttg tttcgcctga gaccttactg catctgcgta aagattcgct tagacggtaa
     1381 ggttaacgaa aggctccaat ccccttattc ccctctcccc gccagcgggg agaggacgag
     1441 agccgagcgt aagcgaaggc gatcgggtga ggggctgtct gacgttcgcg ctcagccgtc
     1501 agacagcccc tcacccgagg atcggcggct acgccttgct cctcgtcctc tcccctcgtg
     1561 gaggggagag gggtaaaaga ggtcttaatc ctgaaacgga tcgcgcatca gaatggtgtc
     1621 ttcccgctca ggcgaggtcg acagcagcgc gaccggcgcg ccgatcagct cctcgatgcg
     1681 gcgcacatac ttaaccgcgg cgccgggcag gtctttccac gaacgcgcac cctgcgtgct
     1741 ttgcgaccag ccttccatct cctcatagac cggctcgatt ccggcttgcg ccttgaaccc
     1801 ggcgggcagg tagtccagca ccttgtcgcc gaccttgtac ccgacgcaga ccttgatcac
     1861 gtcgaggccg tcgagaatgt ccagcttggt cagggcgatg ccgtcgatgc cgttgatggc
     1921 gatcgactga cgcgccagca cggcgtccag ccagccgacg cggcgcgcgc ggcccgtgac
     1981 ggtgccgaat tcatggccgc gctcggcgat gcccttgccg acctcgtcga acagctcggt
     2041 cgggaacggg ccggagccga cgcgcgtggt gtaggccttg aggatgccca gcacatagcc
     2101 gacgcttttc ggccccatgc cggacccggc ggcggcctga ccggccacgg tgttggacga
     2161 ggtgacgaac ggataggtgc cgtggtcgat gtcgagcaag gcgccttgcg cgccctcgaa
     2221 caggatgcgc ttgccctccg tcctggcctt gtccagcacg taccaggccg gtttgacgtg
     2281 cgacagaagg cgcggcgcga tctccctcag cttgtccagc agacccgcga catcgacgtc
     2341 gggcaggccc agcccctgac gcagcggatt gtggtgcgcc agaagccggt cgatcttggc
     2401 gcccagcgcg tcgaaatcct caaggtccgc gacgcggatg gcgcggcggc cgaccttatc
     2461 ctcataggcc gggccgatgc cgcgccccgt ggtgccgatc ttgcccgccc cggcggcggc
     2521 ttcgcgcgcc agatcgagtt cggaatggat cggcaggatc aggcaggcgt tgtcggcgat
     2581 gactaggagg tccggcgtga tgctgaggcc ctgcgcctcg acgcgcgcga tttcggcgaa
     2641 cagggcccac gggtcgatga ccacgccgtt gccgacgatg ctgagcttgc cctgcaccac
     2701 gcccgacggc aaaaggctca gctta
//
LOCUS       DASVLL010000744         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_52441, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000744 DASVLL010000000
VERSION     DASVLL010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52441"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VF402_12205"
     CDS             complement(<1..448)
                     /locus_tag="VF402_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801108.1"
                     /translation="MIGSPLSRWTLAYFACALACLMAALALMAAGFGFPAQSWRDPRT
                     LILVHLVAVGWLSLLMTGALLQFTPVLTARKLACPGLSGPALLISLAGLAALLTGFAA
                     LAGAPVGGPPWLPLGGAGLLTGFGLNLFSLILTCRRAAARPLPVLFV"
     gene            complement(479..727)
                     /locus_tag="VF402_12210"
     CDS             complement(479..727)
                     /locus_tag="VF402_12210"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX7801109.1"
                     /translation="MPEAQTETPVFDVRSLVPAERHRQIFARVEALKPGEGFLLVNDH
                     DPKPLYYQLNAEFPGRFGWEYVTSGPAQWQVLIARNAA"
     gene            972..1406
                     /locus_tag="VF402_12215"
     CDS             972..1406
                     /locus_tag="VF402_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7801110.1"
                     /translation="MHLTTYTDYALRVLIYLNAHVDRRPTIGEIADKYGISKNHLVKC
                     AQQLGRAGFLVNTRGRSGGLALARPADQITLGEVVRRMEPEMDIVPCMDRDNHSCVIS
                     EVCGMKPVFHEAREAFLRVLDGYSVADITRNRLSLLRLLPAA"
     gene            1446..1748
                     /locus_tag="VF402_12220"
     CDS             1446..1748
                     /locus_tag="VF402_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HEX7801111.1"
                     /translation="MTRTIPPEARAYKRTPTFTETSTPEALRKAHSTKAGVWGKLRVT
                     QGRLLYVVDDPAPGAEAVALSPDTGPGIILPQQKHHVEIDGPVAFHVEFYAVAATV"
     gene            1813..2256
                     /locus_tag="VF402_12225"
     CDS             1813..2256
                     /locus_tag="VF402_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801112.1"
                     /translation="MRIVDEGLTPGADLARADTQAPGYGSERQRKFFAGAYGAMVACY
                     AVMMAAFTLVFLHSGVALFNVAIGILYGLMYFGLPVVLTRVARTRDLRFRPAEERHPD
                     LIPIATGAVRPSVAFAQMLLIPAALAICTTALVVVFAVVTAGHQP"
BASE COUNT          409 a          750 c          780 g          376 t
ORIGIN      
        1 gcacgaacag cacaggcagg ggccgcgccg ccgcccggcg gcaggtcagg atcaggctga
       61 acaggttgag cccgaagccg gtcagcagac cggcgccgcc cagcggcagc cacggcgggc
      121 cgccgaccgg cgcgcccgcc agcgcggcga aacccgtcag cagggccgcc agcccggcca
      181 gcgagatcag cagggccggg ccggacaggc cgggacaggc cagtttccgc gcggtcagca
      241 ccggcgtgaa ttgcaacagg gcgccggtca tcaacaggct gagccagccg acggcgacca
      301 gatggacgag gatcagggtg cgcgggtcac gccacgattg cgccggaaag ccgaagcccg
      361 ccgccatcag cgccagcgcc gccatcaggc aggccagcgc gcaggcgaaa taggccaggg
      421 tccagcggga gagaggggag ccgatcatga cgtgaatccc gaatgtggcg cggcccgctc
      481 aggccgcgtt gcgcgcgatc agcacctgcc actgcgcggg gccggatgtg acgtactccc
      541 agccgaaccg gccgggaaat tcggcgttga gctggtaata gagcggcttg gggtcgtgat
      601 cgttgaccag caggaagccc tcgcccggct tcagcgcctc gacgcgcgcg aagatctggc
      661 ggtggcgttc ggcggggacg agggagcgga cgtcgaaaac gggcgtttcg gtttgggctt
      721 cgggcatgat gggcctcgca ggctatgtga tggatgggaa ggtcggccgt attggccgcc
      781 ttaatgatgt attctatata catctttaaa tttgcaaagc cggtatcggc ggtctgcggc
      841 aatatgtacc atcgccttgc ccggttgggc cgggcggcgg acgcaaaggg cggtttgccg
      901 cattggaaat gcatgttaca gtgccgcgtc ccggtcgccc gccgggacgg cgatgaggac
      961 gcgagccctg aatgcacctg acgacctata ccgactacgc gctgcgcgtg ctgatctatc
     1021 tcaacgcgca tgtcgaccgg cgcccgacca tcggcgagat cgccgacaaa tacggcatat
     1081 cgaagaacca tctggtcaag tgcgcgcagc aactgggccg ggcgggcttc ctggtcaaca
     1141 cgcgcggccg cagcggcggg ctggctctgg cgcggcccgc cgaccagatc acgctgggcg
     1201 aggtcgtgcg ccgcatggag ccggaaatgg acatcgtgcc gtgcatggac cgcgacaacc
     1261 acagttgcgt gatctcggag gtctgcggca tgaagccggt tttccacgag gcgcgcgagg
     1321 cgttcctgcg tgtgctggac ggctattccg tcgccgacat cacccgcaac cggctatcgc
     1381 tgctgcgcct gctgcccgcg gcgtgacggc tgtatcctgt atcgcgcatc tatcgattac
     1441 gccgcatgac acgcaccatt ccgcccgagg cccgcgccta taagcgcacg ccgaccttca
     1501 ccgaaaccag cacgcccgaa gcgctcagga aggcgcattc gaccaaggcg ggcgtgtggg
     1561 gcaagctgcg cgtcacgcag gggcggctgc tttacgtcgt cgacgatccc gcgccgggcg
     1621 ccgaggccgt ggcgctgtcg cccgataccg gccccggcat tatcctgccg cagcagaagc
     1681 accatgtcga gatcgacggc ccggtcgcct tccatgtcga attctacgcc gtcgccgcga
     1741 cagtctgacg cattaaacat gcattgaaaa taccataata tgcgcgggtg aaatccgatc
     1801 ggggaggtcg cgatgcgcat agtcgatgaa ggtcttacac ccggcgccga tctggcgcgc
     1861 gccgatacgc aggcgcccgg ctacggctcc gaacgccagc gaaagttttt cgcgggcgcc
     1921 tatggcgcga tggtcgcctg ctacgcggtg atgatggccg ccttcacgct ggtcttcctg
     1981 cattcgggcg tggccctgtt caatgtcgcc atcgggattt tatacggcct catgtatttc
     2041 ggcctgccgg tcgtgctgac gcgcgtggcg cgcacgcgcg acctgcgctt tcgcccggcg
     2101 gaagaacgcc atcccgacct cattccgatc gcgaccggcg cggtcaggcc gtccgtggcc
     2161 ttcgcccaga tgctgctcat ccccgccgct ttggccatct gcacgaccgc gctggtcgtc
     2221 gtgttcgcgg tcgtgaccgc cggtcatcag ccctgaatgg gggattaaag cgtttcccca
     2281 aaaagtggtt ccactttttg gataaggaag agcga
//
LOCUS       DASVLL010000745         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_52475, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000745 DASVLL010000000
VERSION     DASVLL010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52475"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF402_12230"
     CDS             <1..354
                     /locus_tag="VF402_12230"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7801113.1"
                     /translation="KKGVTSLPTLGDGRQSGTSDSPSILNASPESAVGGGLAWLRTGD
                     VIRIDLNRGECNMLVSDDVIAERKKDGLPPVPESMTVWQEIYRATVSQLHTGGVMEPA
                     LKYKRIAEKTPRHNH"
     gene            621..2060
                     /locus_tag="VF402_12235"
     CDS             621..2060
                     /locus_tag="VF402_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEX7801114.1"
                     /translation="MSSTTKTVNMGLVMGLAIGAALGGLLFGYDTAVISGAVDAIDHN
                     FVQPRHELSEIARNAMTGNAVGIALWGCFFGSLIAGPLATALGRRTGMLIAAVIFFAS
                     SILAGYPETGFGGPGSLGVHALTPFMIYRFCGGVAIGMASLISPMYIAEIAPAKQRGL
                     LVTFQQVAIVGGMLLVYFVNLKIAQLGGAGNDAFVLSTGWRYMLASCAIPASLFFLAA
                     LFMPDTPRWYMMKGKEDKARKVLVQLDGVDEAEITLAEIKGSLAQHSGKLFSFGVGVI
                     VVGVLLSVFQQFVGINAVLYYAPLMFKNMGSGTDTALLQTVIVGAANVLFTIVALFTV
                     DLWGRKPLLILGAVIMAVAMFALGVLFQHHTEGNLLLFSAILYIAGFAMSWGPVVWVL
                     LSEMFPNSIKGPAMAIAVAAQWISNYVVTASFKVVDGDTHINAMFNHGLNYYVYGVCS
                     VLAALFVWKFVPETKNRRLEEIEALWKKH"
     gene            2138..>2807
                     /locus_tag="VF402_12240"
     CDS             2138..>2807
                     /locus_tag="VF402_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX7801115.1"
                     /translation="MLRLNAGDSVLDLNPDLGGAIARFTHKGADVMRPTPDDASDVLQ
                     TACFPLVPFCNRIRDGKFVVEGHKVQLSPNLNDSPHTLHGQGWRHAWRVVEASASRAV
                     LSYRHAADEWPWDYEATQVFELREKGLRVYLDVVNRSRGHMPAGLGFHPYFNLTDQTR
                     LKAHVDGVWIADDELLPRNWHAGVLFKNWTRGEALRFDKLIDNCYTGFRGRAEIFEGS
                     RMTHA"
BASE COUNT          494 a          930 c          869 g          514 t
ORIGIN      
        1 aagaagggcg tcacctcgct gccgacgctc ggcgacgggc gccagtccgg cacctcggac
       61 tcgccgtcca tcctcaacgc ctcgccggaa agcgcggtcg gcggcggtct ggcctggctg
      121 cgcaccggcg acgtcatccg catcgacctc aacaggggcg agtgcaacat gctggtcagc
      181 gacgacgtga tcgcggaacg taaaaaggac ggcctgccgc cggtccccga atcgatgacc
      241 gtctggcagg aaatctaccg cgccacggtc tcgcaactgc acaccggcgg cgtgatggag
      301 ccggccttga aatacaagcg catcgccgag aaaacgccgc gccacaacca ctgagcggga
      361 ccggaaacgg ggcgcgaatt gttgcgccgc atatatggta gcgttaacag ccatgcgtcc
      421 gtttcgacat ccgaaacgga cgcaaagcct taaaatatct cgcttctttg gatacggacg
      481 cttgcgcggc gcccagcctg ccattaagag ttcattaagc ggcagtataa acacgagcgg
      541 acgagcagaa tccgcaaggc cacataagcc gcgaacttca aggtccgggc aggaagcccg
      601 caggtaggga gaatttgagg atgtcctcaa ccacaaagac cgtgaacatg ggtctggtca
      661 tggggctcgc catcggcgcg gctctgggcg ggcttctgtt cggttacgac accgccgtga
      721 tttccggcgc cgtcgacgcc atcgatcata atttcgtgca gccgcgccat gaactcagcg
      781 aaatcgcgcg caacgccatg accggcaacg cggtcggcat cgccctgtgg ggctgcttct
      841 tcggctcgct gatcgccggg ccgctggcca ccgcgctggg ccgccgcacc ggcatgctga
      901 tcgccgccgt catcttcttc gcctcgtcga ttctggcggg ctatcccgaa accggcttcg
      961 gcggtccggg cagcctgggc gtccacgccc tgaccccgtt catgatctac cgcttctgcg
     1021 gcggcgtcgc catcggcatg gcctcgctga tctcgcccat gtacatcgcc gaaatcgcgc
     1081 ccgccaagca gcgcggcctg ctcgtgacct tccagcaggt cgccatcgtc ggcggcatgt
     1141 tgctggtcta tttcgtcaac ctcaagatcg ctcaactggg cggcgcgggc aacgacgcct
     1201 tcgtgctcag caccggctgg cgctacatgc tggccagctg cgccatcccg gccagcctgt
     1261 tcttcctggc ggcgctgttc atgccggaca cgccgcgctg gtacatgatg aagggcaagg
     1321 aagacaaggc gcgcaaggtg ctggtccagc tcgacggcgt ggacgaggcc gagatcaccc
     1381 tggccgaaat caagggcagc ctggcccagc attcgggcaa gctgttctcg ttcggcgtcg
     1441 gcgtgatcgt ggtcggcgtg cttctgtcgg tgttccagca gttcgtcggc atcaacgccg
     1501 tgctgtatta cgcgccgctg atgttcaaga acatgggctc cggcaccgat acggcgctgc
     1561 tgcaaaccgt gatcgtcggc gcggccaacg tgctgttcac catcgtcgcc ctgttcaccg
     1621 tcgacctgtg ggggcgcaag ccgctgctga tcctcggcgc cgtcatcatg gcggtggcca
     1681 tgttcgcgct cggcgtgctg ttccagcatc ataccgaagg caatctgctg ctgttctcgg
     1741 ccatcctcta catcgcgggc ttcgccatgt cgtggggtcc ggtcgtgtgg gtgctgctgt
     1801 cggaaatgtt cccgaactcg atcaagggtc cggccatggc catcgccgtc gccgcgcagt
     1861 ggatctccaa ctatgtcgtc accgcctcgt tcaaggtggt cgacggcgac acccacatca
     1921 acgccatgtt caatcacggc ctgaactact acgtctatgg cgtctgctcg gtcctggccg
     1981 ccttgttcgt ctggaagttc gtgccggaaa ccaagaaccg ccgtctcgaa gagatcgagg
     2041 cgctgtggaa gaagcactga gtcttgcgta accagggggg cggagtccgg ccacggactg
     2101 cgcccccctt cttttttaag cgacgaaagg caggataatg ctgcgcttaa acgccgggga
     2161 cagcgtcctc gatctcaatc ccgacctcgg cggcgcgatc gcgcgcttca cgcacaaggg
     2221 cgccgacgtg atgcgcccga cgccggacga cgccagcgac gtgctgcaaa ccgcctgttt
     2281 tccgctggtt ccgttctgca accgcatccg cgacggcaag ttcgtcgtcg agggccataa
     2341 ggtccagctt tccccgaatc tgaacgacag cccacacacc ctgcacggtc agggctggcg
     2401 ccacgcctgg cgggtggtcg aggcgtcggc ctcgcgcgcg gtcctcagct atcgccacgc
     2461 cgccgacgaa tggccgtggg actatgaggc gacgcaggtg ttcgaactgc gcgaaaaggg
     2521 cctgcgcgtc tatctcgacg tcgtcaaccg ctcgcgcggc cacatgcccg ccggtctggg
     2581 ctttcacccc tatttcaacc tgacggacca gacgcggctc aaggcgcatg tcgacggcgt
     2641 gtggatcgcc gacgatgagc ttctgccgcg caactggcac gcgggcgtcc tgttcaagaa
     2701 ctggacgcgc ggtgaggcgc tgcgcttcga taagctgatc gacaattgct acaccggctt
     2761 tcgcggccgc gccgaaatct tcgaggggtc gcgcatgacg cacgccc
//
LOCUS       DASVLL010000746         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_52878, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000746 DASVLL010000000
VERSION     DASVLL010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52878"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VF402_12245"
     CDS             complement(<1..549)
                     /locus_tag="VF402_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7801116.1"
                     /translation="MKKLILLLGAPLLLSACVKPADKAPASSASASEAAPDVGKLPAG
                     TYRSDPAHTSLTFEVDHLGFSHYTARFAKIGAQMAFDPAHPEAAQLTALIDPRSLQLN
                     APPAGFHDALMGKDWFGAAQFPDIRFVSTQVARTGPATADVSGNLSLHGVTKPVVLQV
                     RFNGGYPGLPGYDPNARIGFSAT"
     gene            complement(546..1019)
                     /locus_tag="VF402_12250"
     CDS             complement(546..1019)
                     /locus_tag="VF402_12250"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7801117.1"
                     /translation="MTRSMTPATFECERDFAHSVALLYWAFTDLEARKVWYGGRERGF
                     NVAHHTLDFRAGGAESWRGQPHGAAWMTNDATFIEILPEQRVIYSYVMTMDGALFTVS
                     QQAVEFSARGTGSHLKLTEQIMFIDRTDHLAQRIEGTQAMLDHLNAWIDNRETTL"
     gene            complement(1024..1542)
                     /locus_tag="VF402_12255"
     CDS             complement(1024..1542)
                     /locus_tag="VF402_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7801118.1"
                     /translation="MPLEQPASEAEVFTIERVFDASPELLWEVCTKAGHLSQWFGPPG
                     TTVKVKTLDLKPGGVFLYGMEMPGGIVMWGKWVFREIDAPHSLAYVVSFCAEDGTPVR
                     HPMAPLWPLEVLAVQTFEARNGKTLMKSRSWPINATAEERAVFTAGHASMQMGFGGAW
                     MQLDAYLNRLKG"
     gene            complement(1555..1899)
                     /locus_tag="VF402_12260"
     CDS             complement(1555..1899)
                     /locus_tag="VF402_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7801119.1"
                     /translation="MPEVHDPLSQTLTALADPTRRAILARLTHGEASVTELAEPFAMS
                     LPAVSKHLKVLEKAGLISRGREAQWRPCKLEPAPLQAVDAWLGDYRKMWEERLDRLDA
                     YLKTLKPDGELH"
     gene            complement(1973..>2444)
                     /locus_tag="VF402_12265"
     CDS             complement(1973..>2444)
                     /locus_tag="VF402_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7801120.1"
                     /translation="AFCADAEAMKHLGGAQTPPMVWRMLTSVVGAWHLYGFHMFSVLD
                     KASGAWIGRIGPLQPFGWPGREVGWGLMTAHQGKGYAREAAVACMDFVFDRLGWDEVI
                     HTINPGNRPSAALAAALGSVNRGPGQLPDPYAGERVDIWGQSRADWRENRKRFL"
BASE COUNT          403 a          783 c          835 g          423 t
ORIGIN      
        1 ggtggcggaa aagccgatgc gcgcgttcgg atcgtatccg ggcaggcccg gatagccgcc
       61 gttgaagcgc acttgcagca cgaccggctt cgtcacgcca tgcaggctca gattgccgct
      121 tacgtccgcc gtcgccgggc cggtgcgcgc gacctgggtc gagacgaagc ggatgtcggg
      181 aaactgcgcg gcgccgaacc agtccttgcc catcagcgcg tcgtggaagc ccgcaggcgg
      241 cgcgttgagt tgcagggacc gcggatcgat gagcgcggtc agttgcgcgg cttccgggtg
      301 ggccgggtcg aacgccatct gcgcgccgat tttcgcgaac cgcgcggtgt agtgcgagaa
      361 gccgaggtga tcgacctcga aggtcagcga ggtgtgcgcc ggatcgctgc ggtacgtccc
      421 ggcgggcagt ttgccgacgt ccggcgcggc ttcggacgca gaggcggatg aagcgggcgc
      481 cttgtcggcg ggtttgacgc aggcgctcag caggagcggc gcgcccagca gcaggatcag
      541 ctttttcata acgtcgtctc ccgattgtcg atccaggcgt tcaggtggtc cagcatggcc
      601 tgcgtgcctt cgatgcgctg cgccagatgg tcggtccggt cgatgaacat gatctgttcg
      661 gtcagtttca ggtgcgatcc ggtcccgcgc gcggaaaact ccaccgcctg ctgcgacacg
      721 gtgaacaggg cgccgtccat cgtcatgaca tagctgtaga tgacgcgctg ttccggcagg
      781 atttcgatga aggtcgcgtc attggtcatc caggcggcgc cgtgcggctg gccgcgccac
      841 gactcggcgc cgcctgcgcg gaaatcgagc gtgtgatgcg cgacgttgaa gccgcgctcc
      901 cgtccgccat accagacctt gcgcgcctcc agatcggtga aggcccagta cagaagcgcc
      961 acggaatgcg cgaagtcgcg ttcgcactca aaggtggcgg gggtcatgct gcgggtcatg
     1021 agtctagcct ttcaaccggt tcagataggc gtcgagttgc atccaggcgc cgccgaagcc
     1081 catttgcatc gaggcgtggc cggcggtgaa gacggcgcgt tcctccgccg tggcgttgat
     1141 cggccacgag cggcttttca tcaaagtctt gccgtttcgc gcctcaaagg tctgcacggc
     1201 cagcacctcc agcggccata gcggcgccat cggatgccgc accggcgttc cgtcctccgc
     1261 gcagaaggag acgacatagg cgaggctgtg cggcgcgtcg atctcacgaa acacccactt
     1321 gccccacatg acgatcccgc cgggcatctc catgccgtag aggaagacgc cgcccggttt
     1381 cagatcgagc gtcttgacct tgacggtcgt tcccggagga ccgaaccact ggctgagatg
     1441 cccggccttg gtgcagacct cccacaacag ctcgggcgag gcgtcgaaga cgcgctcgat
     1501 ggtgaagacc tcggcctcgg aggccggttg ttcgaggggc atcgcgtctc tctcctaatg
     1561 cagttcgccg tcgggcttta aggttttcag gtaggcgtcg aggcggtcga gcctctcctc
     1621 ccacattttg cgatagtcgc cgagccaggc gtcgaccgcc tgcaacggcg cgggctccag
     1681 cttgcacggg cgccattgcg cctcgcggcc gcgcgagatc aggcccgcct tttccagcac
     1741 tttcagatgc ttggagacgg cgggcaggct catggcgaac ggctcggcca gttcggtcac
     1801 cgacgcctcg ccgtgcgtca gccgggccag aatggcgcgg cgcgtcggat cggccagcgc
     1861 ggtcagggtc tggctgagcg gatcatgaac ctccggcatg gcattaaact catgagttaa
     1921 ataactgatt ggttaaatac tcgcgacatc cgcgtgagtc aaggcggcgt cgctacaaaa
     1981 agcgcttgcg gttttcgcgc cagtcggcgc ggctctggcc ccagatgtcg acgcgctcac
     2041 cggcgtaagg gtcgggcagt tgtccggggc cgcgattgac cgagccgagc gcggcggcca
     2101 gcgccgccga gggccggttg ccgggattga tggtgtgaat gacctcgtcc cagccgagcc
     2161 ggtcgaacac gaagtccatg caggcgacgg cggcttcgcg ggcgtagccc ttgccctgat
     2221 gcgcggtcat caggccccag ccgacctccc ggcccggcca gccgaacggt tgcagcgggc
     2281 cgatgcggcc gatccaggcg cccgacgcct tgtccagcac gctgaacatg tggaagccat
     2341 aaagatgcca ggcccctacc accgaggtca gcatgcgcca gaccatgggc ggggtctgcg
     2401 cgccgccgag atgcttcatc gcctctgcgt cggcgcagaa ggcg
//
LOCUS       DASVLL010000747         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_52944, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000747 DASVLL010000000
VERSION     DASVLL010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52944"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF402_12270"
     CDS             <1..593
                     /locus_tag="VF402_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801121.1"
                     /translation="PASDSASPHAAASRRADLKVCGECHLCCKVYDIPDFEKKAGRVC
                     QHVRTEGGCGVWGLHPQICQDFKCLWLKHADMNALWRPDVCGFVLRVEPNGTTLAVDV
                     DPDRPDAWRQAHYYEQIKLWSEVMPKNEGLVLVYAPEGLYVVTPMEDLFLKTPRRGDV
                     LETGMEPSLFGLRPFARVVPARERRRERFDGVPRRA"
     gene            635..1639
                     /gene="ubiA"
                     /locus_tag="VF402_12275"
     CDS             635..1639
                     /gene="ubiA"
                     /locus_tag="VF402_12275"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX7801122.1"
                     /translation="MSSALQNQLPDAARLNWVDRYAPAALRPWLRLGRFDRPIGIWLL
                     YLPCAMGLALGAAHPVFAIDAFDPGVARPHWDARAAFYAFPYLKLLLFFVGAALMRAA
                     GCAFNDIVDRDFDARVERTRGRPIPSGQISPKQALAFIVVCSLISLAILLQLGRLAVI
                     LGVASLGLVAAYPFMKRITWWPQAWLGLTFNWGLLMGYATTTGRIDWPVLVFYAGLVF
                     WTLGYDTIYALQDIDDDIMIGVKSSARALGRHTVAGVSVFYGLAVVLTGAAGMLAHLP
                     FLYFAGLLALTGHFYWQVVRLNPDDGALALRLFKSNRTAGLIWFVSLLLVHVSGGFHV
                     "
BASE COUNT          245 a          619 c          591 g          337 t
ORIGIN      
        1 cgcccgcatc cgattccgcg tcgccccatg ccgcggcgtc gcggcgcgcc gatctcaagg
       61 tctgcgggga atgccatctg tgctgcaagg tgtacgacat ccccgacttc gagaagaagg
      121 ccggtcgggt ctgccagcac gtgcggacgg aaggcggttg cggcgtctgg ggcctgcatc
      181 cgcaaatctg tcaggatttc aaatgcctgt ggcttaagca cgccgatatg aacgcgctgt
      241 ggcggcccga tgtctgcggc ttcgtgctgc gcgtcgagcc gaacggcacg accctggctg
      301 tcgatgtcga tcccgaccgc ccggacgcct ggcggcaggc gcactattac gagcagatca
      361 agctgtggtc cgaggtcatg ccgaagaacg aggggctggt cctcgtctat gcgccggaag
      421 ggctttacgt cgtcacgccg atggaggatc tgttcctgaa aacgcccagg cgcggcgacg
      481 ttctggaaac cggcatggag cccagcctgt tcggcctgcg tccgttcgcg cgcgtcgtcc
      541 cggcccgcga acgccgccgg gaacgcttcg acggcgtgcc gcgccgggcc taaaaaggct
      601 cgacttttta ccgcgggcag tccattcaaa gggcatgtcc agcgccctgc aaaatcagct
      661 tcccgacgcc gcccgcctga actgggtcga ccgttacgcg cccgccgccc tgcgtccgtg
      721 gctgcggctg gggcgcttcg accggccgat cggcatatgg cttttgtacc tgccgtgcgc
      781 gatggggctg gcgctcggcg cggcgcatcc ggtcttcgcc atcgacgcct tcgatcccgg
      841 cgtcgcgcgg ccgcactggg acgccagggc cgcgttttac gcctttccct acctgaaact
      901 gctgctgttc ttcgtcggcg cggcgctgat gcgcgcggcg ggctgcgcgt tcaacgacat
      961 cgtcgaccgc gatttcgacg cccgcgtcga gcgcacgcgc ggccgcccga tcccgtccgg
     1021 ccagatcagc ccgaaacagg cgctggcctt catcgtcgtc tgttcgctga tctcgctggc
     1081 catcctgctc cagctcgggc ggctggccgt cattctcggc gtcgcctcgc tggggctggt
     1141 ggcggcctat ccgttcatga agcgcatcac ctggtggccg caggcgtggc tgggcctgac
     1201 cttcaactgg gggcttctga tgggctatgc gacgacgacg ggccgcatcg attggcccgt
     1261 cctcgtcttc tacgccgggc tcgtcttctg gacgctgggc tacgacacca tctacgcctt
     1321 gcaggacatc gacgacgaca tcatgatcgg ggtcaagtcc tcggcgcgcg cgctggggcg
     1381 ccacaccgtc gcgggcgtca gcgttttcta cggcctcgcc gtcgtgctga ccggcgcggc
     1441 ggggatgctg gcccacctgc ccttcctcta tttcgcgggc cttctggcgc tcaccggcca
     1501 cttctactgg caggtcgtcc gcctgaaccc ggacgacggc gcgctggcgc tgagactgtt
     1561 caagtccaat cgcacggcgg ggctgatctg gttcgtcagc cttctgctgg tgcatgtcag
     1621 cggcggtttc catgtttagg ttcgcgtccg gcctcacccg catctgcgtc gcggcgctcg
     1681 tcgtggcggc gctgagcggc tgccataaaa aagccgaccg ggaggccgag ccgaaaccgc
     1741 ccgccagcgt ggcgccgctc ggcttcgccc agaccaatga cgacgcgcag gt
//
LOCUS       DASVLL010000748         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_53494, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000748 DASVLL010000000
VERSION     DASVLL010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53494"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VF402_12280"
     CDS             <1..775
                     /locus_tag="VF402_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX7801123.1"
                     /translation="VSGLLIRPLHIDDDMPLMVFFHQGGGVLGGPELSRAFGGLFVHA
                     ARCPVFLPHYRLAPDHRFPAALEDAHKIADWAQTNAFTLGAPSGRVALGGALTGAGLA
                     ARLCLELKRDFKPLPAGQLLLTPLLDLGDPDLGAADKGSWPLNGADLAEMIADYAGGG
                     HDLSDPRLSPGREKLLGGQPPGLIVSAGFDATAAQAEAYARRLRDAGCEVVYRRHDTL
                     PLGFDLLCGLAGSARAAILATAELWRGLMRRSRTAGDAS"
     gene            906..1283
                     /locus_tag="VF402_12285"
     CDS             906..1283
                     /locus_tag="VF402_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX7801124.1"
                     /translation="MKRLLTLAAAAALIGGTALATAAEAAPPYDHGRYDHYDRHDRYD
                     RHDRYDRYANHRWNYDRGYRHSHWVRGGYVPRDYWGRGRRVDYRYYHLRPPPRGYEWR
                     YVDGDYVMASIATGLIASIVLAQ"
     gene            complement(1317..3248)
                     /gene="dxs"
                     /locus_tag="VF402_12290"
     CDS             complement(1317..3248)
                     /gene="dxs"
                     /locus_tag="VF402_12290"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7801125.1"
                     /translation="MPDTPILDRPVLDKLGDPAAIHALSGDELRQLADDVRAETIDVV
                     SRTGGHLGSALGVVELSVALHHVFDTPRDILIWDVGHQTYPHKILTGRRGRMRSLRQG
                     GGLSGFTRRSESPYDPFGAAHAATSISAALGFCAARDHKGEDYKVVAVIGDGSLSAGM
                     AYEAMNNAAETTKNLTVILNDNDMSIAPPVGGMSAYLANLVSGGAYQKLRRWGKSMAE
                     RLPRPLFEMVRKGEEFSRTYVTGGTFFEELGFYYVGPIDGHNMEHLLTVLKRVHGIVD
                     RPVLVHVVTQKGKGYAPAETAADKYHGVSKFDVVTGEQARPKANAPSYTDVFASELIK
                     QARADDRIVAITAAMPSGTGLDRFAEVFPTRCYDVGIAEQHAVTFAAGLAADGMKPFC
                     ALYSTFLQRGYDQVAHDVSLQNLPVRFAIDRAGLVGADGATHAGAFDIGYLGALPNMM
                     LMAASDEAELAAMIATATAYDDGPSAFRYPRGEATGAAIPDHAQPLEIGKGRIVREGS
                     KVAILSLGTRLADALKAADLLAARGFSTTVADARFAKPLDTGLIAQLARHHECLITVE
                     EGAVGGFGAFVLHYLAAEGALDHGLRIRSLTLPDRYQDQDSQANQYAEAGLDARHIAL
                     TALRALGLDEAVAVKALGK"
     gene            complement(3291..>3663)
                     /locus_tag="VF402_12295"
     CDS             complement(3291..>3663)
                     /locus_tag="VF402_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7801126.1"
                     /translation="VARMQRLKTGALIVYAFQIPLVVADASDRDGLALTHFAQDIGLA
                     YQIADDVLDIEGDPEELGKGAGGKDAAKGKTNFVTLLGLEEAKARVRILSDQAKSRLD
                     IFGSDARYLRESVDFVLNRRK"
BASE COUNT          562 a         1219 c         1279 g          603 t
ORIGIN      
        1 cgtcagcggg cttctgatcc ggccgctgca tatcgacgac gacatgccgc tgatggtgtt
       61 tttccatcag ggcggcggcg tgctgggcgg gccggagctg agccgcgcct tcggcgggct
      121 gttcgtccat gcggcgcgct gcccggtctt tctgccgcat taccggctgg cgcccgacca
      181 ccgctttccg gcggcgctgg aggacgcgca caaaatcgcc gactgggcgc agaccaacgc
      241 cttcacgctg ggggcgccgt cggggcgcgt ggccttgggc ggcgcgctga ccggcgccgg
      301 gctggcggcg cgcctgtgcc tggagctgaa acgcgatttc aagccgctgc ccgccgggca
      361 gttgctgctg acgccgctgc tcgatctggg cgatcccgat ctgggcgcgg ccgacaaggg
      421 atcgtggccg ctgaacggcg ccgatctggc cgagatgatc gccgactatg cgggcggcgg
      481 ccacgacctc agcgatccgc gcctgtcgcc ggggcgcgaa aagctgctgg gcggccagcc
      541 gcccggcctg atcgtgtcgg cgggcttcga cgcgaccgcc gcgcaagcgg aagcctacgc
      601 cagacggctg cgcgacgccg ggtgcgaggt cgtttaccgg cgccacgaca cactgccgct
      661 ggggttcgac ctgctgtgcg ggctggccgg gtcggcgcgg gcggcgattc tggcgacggc
      721 cgagctgtgg cgcggcctga tgcggcgcag ccgcacggcc ggcgacgcat cctgatctta
      781 cccgttcggc tttcctgacg cattgcccgc ttaacccatt catcgcacgt tcagcgggcg
      841 tatcctatca tgcgtgatat ggggaatacg gggattgacc ggggcagacc ggaaaggaca
      901 cgaacatgaa gcgtcttttg accctcgccg ccgccgcggc cctgatcggc gggaccgcgc
      961 tggcgaccgc cgccgaggcc gcgccgccgt acgatcatgg ccgttacgac cactatgacc
     1021 gtcatgaccg ctatgaccgc catgaccgct acgaccgtta cgccaaccat cgctggaatt
     1081 acgatcgcgg ctatcgccac agccactggg tgcgcggcgg ctatgtgccg cgcgattact
     1141 gggggcgcgg gcgccgcgtc gactatcgct actatcacct gcgtccgccg ccccgcggct
     1201 atgaatggcg ctatgtcgac ggcgattacg tgatggccag catcgccacc ggcctgatcg
     1261 cttcgatcgt gctggcgcaa taaaccaaga ggcggctccg ggaacggagc cgctttttac
     1321 ttgcctaaag ccttgaccgc cacggcctcg tccagcccca gggcgcgcag cgcggtcagg
     1381 gcgatatggc gcgcgtcgag gcccgcctcg gcgtactggt tcgcctggct gtcctgatcc
     1441 tgatagcggt cgggcagggt caggctgcgg atgcgcaggc cgtggtccag cgcgccttcc
     1501 gccgccagat agtgcagcac gaaggcgccg aagccgccca ccgcgccctc ctccaccgtg
     1561 atcaggcatt catgatgccg cgccagttgc gcgatcaggc cggtgtcgag cggcttggcg
     1621 aagcgcgcgt cggccaccgt ggtcgaaaag ccgcgcgccg ccagaaggtc ggcggccttg
     1681 agcgcgtcgg cgaggcgcgt gcccagcgac aggatggcga ccttggagcc ttcgcgcacg
     1741 atgcggccct tgccgatctc aagcggctgc gcatggtcgg ggatggcggc gcccgtggcc
     1801 tcgccgcgcg gatagcgaaa ggcgctgggg ccgtcgtcat aggccgtggc ggtggcgatc
     1861 atggcggcca gttcggcctc gtccgaggcg gccatcagca tcatgttcgg cagggcgccc
     1921 agatagccga tatcgaacgc gcccgcatgg gtggcgccgt ccgcgccgac cagcccggcg
     1981 cggtcgatgg cgaagcgcac cggcaggttc tgcaaggaca catcgtgcgc gacctgatcg
     2041 tagccgcgct gcaaaaaggt cgaatagagc gcgcagaacg gcttcatgcc gtcggcggcg
     2101 aggcccgccg cgaaggtgac ggcgtgctgc tcggcgatgc cgacatcgta gcagcgggtc
     2161 ggaaagacct cggcgaagcg gtcgaggccg gtgccggacg gcatggcggc ggtgatggcg
     2221 acaatgcggt cgtccgcgcg cgcctgcttg atcagttccg aggcgaagac gtcggtatag
     2281 ctgggggcgt tggccttggg cctggcttgc tcgccggtca cgacgtcgaa tttcgacacg
     2341 ccgtgatact tgtcggcggc ggtctcggcg ggggcgtagc ccttgccctt ctgcgtcacc
     2401 acatgcacca gcaccgggcg gtcgacgatg ccgtggacgc gctttaagac ggtgagcaga
     2461 tgctccatat tgtgcccgtc gatcgggccg acatagtaga agcccagctc ctcgaagaag
     2521 gtgccgccgg tgacgtaggt gcgggaaaat tcctcgccct tgcgcaccat ctcgaacagc
     2581 gggcgcggca ggcgctcggc catgctcttg ccccagcggc gcagcttctg ataggcgccg
     2641 ccggacacga gattggccag ataggcgctc atgccgccga ccggcggggc gatcgacatg
     2701 tcgttgtcgt tgaggatgac ggtcaggttc ttggtcgtct cggcggcgtt gttcatcgcc
     2761 tcataggcca tgcccgccga cagcgagccg tcgccgatca ccgccacgac cttgtaatcc
     2821 tcgcccttat ggtcgcgcgc ggcgcagaag ccgagcgccg ccgagatcga ggtcgccgcg
     2881 tgcgccgcgc cgaacggatc gtaggggctt tccgagcgcc gggtgaagcc ggacaggccg
     2941 ccgccctgac gcaaggagcg catgcggccg cgccgcccgg tcaggatctt gtgcggatag
     3001 gtctgatgcc cgacgtccca gatcaggatg tcgcgcggcg tatcgaagac atggtgcagc
     3061 gccacgctca gctcgaccac gcccagcgcc gagccgagat gcccgccggt gcgcgacacg
     3121 acgtcgatcg tctcggcgcg cacgtcgtcg gccagttggc gcagttcgtc gccgctcagg
     3181 gcgtgaatcg cggcgggatc gcccagttta tcaagaacgg gcctgtcgag gatgggggta
     3241 tcgggcatcg gatctcggtt accggtcggg ctgtcgtccc ggaaaccggc tcatttgcgc
     3301 cgattgagaa cgaagtcaac actctcgcgc aagtatcgcg cgtcactacc gaaaatatca
     3361 agccgggatt tggcctgatc cgataaaatc cgcacgcgcg ccttggcttc ctcaaggccc
     3421 agaagggtga caaagttggt cttgcccttc gccgcgtcct tgccgcccgc ccccttgccc
     3481 agttcctcgg ggtcgccctc gatgtcgagc acgtcgtcgg cgatctggta ggccaggccg
     3541 atatcctgcg cgaaatgggt cagggccagg ccgtcgcggt ccgaggcgtc ggcgaccacc
     3601 agcggaatct ggaacgcata gacgatcagg gcgccggtct tgagcctttg catccgcgcc
     3661 acg
//
LOCUS       DASVLL010000749         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Asticcacaulis sp. isolate SMAG_U3072 k141_53608, whole
            genome shotgun sequence.
ACCESSION   DASVLL010000749 DASVLL010000000
VERSION     DASVLL010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959702
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Asticcacaulis sp. (soil metagenome)
  ORGANISM  Asticcacaulis sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:48:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,461
            CDSs (total)                      :: 2,440
            Genes (coding)                    :: 2,432
            CDSs (with protein)               :: 2,432
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 3 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Asticcacaulis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53608"
                     /isolate="SMAG_U3072"
                     /isolation_source="soil"
                     /db_xref="taxon:1872648"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VF402_12300"
     CDS             <1..430
                     /locus_tag="VF402_12300"
                     /inference="COORDINATES: protein motif:HMM:NF023958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7801127.1"
                     /translation="FAGVPLGNQSVLLKGVNDDADVMKALVHRLLRMRVRPYYLYQMD
                     LITGGSHFKVDVRKGIEIIRALRGHTTGYAVPQYVIDAPGGGGKVPINPDYVEKIDDD
                     EVVFRNYEGKTFRYPSKSTPKLSPDVVPVTADGVEPITLP"
     regulatory      999..1112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1179..>2180
                     /locus_tag="VF402_12305"
     CDS             1179..>2180
                     /locus_tag="VF402_12305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7801128.1"
                     /translation="MKFPSLLLFPLAALLAGPARAQTPPPAAPEEDVTVLSRYIVRDV
                     PPEKSVNPLTRETSAVMGDGRGPLETPRGVSTITTALFNERQIHGVQEILLYSPSAYV
                     GASYGNITTPNLRGDLAETYLNGQRLSYNYYGYFPSFNGVEAVDLVRGPGSVVFGAGY
                     FTGGYVNYVTKRPKFSGPETTITTRVGTWAPGQVSFRNGSVQIDSTAPVNDRLAWRFS
                     YEAKGGETFFRKNDAPDDRQDFFLALTWKPDPATTLEFNAQYFWQNPPETLGVNRVTQ
                     DLIDHGLYYTGASADSVPYPGPIPATGMVPLPWEATLFSRGDFSNANVVRGQLVATHV
                     "
BASE COUNT          375 a          708 c          664 g          433 t
ORIGIN      
        1 ctttgcgggc gtgccgctcg gcaaccagag cgtcctgctc aaaggcgtga acgacgatgc
       61 cgacgtgatg aaggcgctcg tccaccggct gttgcggatg cgggtgcgtc cctactatct
      121 ttatcagatg gatctcatca cgggcggctc gcacttcaag gtcgatgtgc gcaaaggcat
      181 cgagatcatt cgcgcgctgc gggggcacac gaccggttat gcggtgccgc aatacgtgat
      241 cgatgcgccg ggtggcggcg gcaaagtgcc gatcaatccc gactacgtcg aaaaaatcga
      301 cgacgacgaa gtggtttttc gaaattacga gggaaaaacc ttccgatacc cgtcgaagag
      361 cacgccgaag ctttcgcccg acgttgtccc ggtgacagcc gacggggtcg agccgataac
      421 cctgccttga ggcgttgagc tgtcggtgct gcctgcgcga gctctttccg ggcggcggtg
      481 gctcggcgag gagggggccg ttgatctccc ccaatgcctt gggcacctcg gtggctaaga
      541 attggaaggg agcccatgta gccggcctcg ctgaggccgg tccggggtct ggcgatcccg
      601 gcggcaacca aactgtcccg ccggaggtcg gcggccgcca tgccggctgc tcgatctgct
      661 gcgctgatta tttttccgct ttttttgagc gtttcgcgcg caccctcgtc tatccccgcg
      721 tagtcagcac tgtccgcaag gatggtgaat gtttgtagtt cttacggttt gcttggtgtt
      781 aggtcacttg ggccgcctcg aaagaggtgg cccttgtgtt ttcatgggca catctcagtt
      841 tttgtttgat ggagtgccgg gttgagcgcc gcagaaggca cgccgcaagc ggcagcccta
      901 cgagggcgaa acaggctatg accaaaaact gagatgcgcc ttgtttcatg atcctcaaaa
      961 ctttggcttg catcgcagcc gggcggacgt ttttacgagc ctgcctgtag gggtgtcctc
     1021 cgcggagggc tgagattgcg gtgcgctcga ccgctcgacc cttcgaacct gaaacggatc
     1081 atgccgacga aggaaacagc aggcgccgtt ggtcgcgccg cagggcgcac ggcgcccctt
     1141 ttttcgggag tccagtttgg gtcgggcctc agccacctat gaaattcccg tcactccttc
     1201 tctttccgct ggccgcgctg ctggccggcc ctgcccgcgc ccagacgccg ccgcccgccg
     1261 cacccgaaga ggacgtcacc gtgctctcgc gttacattgt ccgcgatgtg ccgcccgaaa
     1321 aatccgtcaa cccgctcacg cgggagactt ccgcggtcat gggcgacggc cgcggtccgc
     1381 tggagacgcc gcgcggcgtg agcacgatca cgaccgcgct gttcaacgag cgccagatcc
     1441 acggcgtgca ggagattctg ctctactcgc ccagcgccta tgtcggcgcc tcctacggca
     1501 acatcacgac accgaatctt cgcggcgacc tcgccgagac gtatctcaac ggccagcggc
     1561 tgagctataa ttattatgga tactttccgt cgttcaacgg cgtcgaggcg gtcgatctcg
     1621 tccgcggccc cggctcggtc gtgttcggcg ccggttattt taccggcggt tatgtgaact
     1681 acgtcacgaa acgcccgaaa ttttccggtc cggaaacgac catcacgaca cgcgtcggca
     1741 cctgggcgcc ggggcaggtt tctttccgca acggctccgt gcagatcgac agcaccgcgc
     1801 cggtgaacga ccgtcttgcc tggcggttca gctacgaggc gaaaggcggg gagaccttct
     1861 tccggaaaaa cgacgcgccc gacgaccggc aggatttttt cctcgccctc acctggaaac
     1921 ccgatcccgc caccacgctg gagttcaacg cccagtattt ctggcagaac ccgcccgaga
     1981 ccctcggcgt caaccgcgtc acccaggacc tgatcgacca cgggctgtac tacaccggcg
     2041 cctccgccga ctccgtgccc tatcccggtc cgatcccggc caccgggatg gtgcctctgc
     2101 cgtgggaggc cacgctcttt tcccgcggcg atttctccaa cgccaacgtc gttcgcggcc
     2161 agctcgttgc cacgcacgtc
//
